title: Lysosomal protease expression in mature enamel.
abstract: The enamel matrix proteins (amelogenin, enamelin and ameloblastin) are degraded by matrix metalloproteinase-20 and kallikrein-4 during enamel development and mature enamel is virtually protein free. The precise mechanism of removal and degradation of the enamel protein cleavage products from the matrix, however, remains poorly understood. It has been proposed that receptor-mediated endocytosis allows for the cleaved proteins to be removed from the matrix during enamel formation and then transported to the lysosome for further degradation. This study aims to identify lysosomal proteases that are present in maturation-stage enamel organ. RNA from first molars of 11-day-old mice was collected and expression was initially assessed by RT-PCR and then quantified by qPCR. The pattern of expression of selected proteases was assessed by immunohistochemical staining of demineralized mouse incisors. With the exception of cathepsin G, all lysosomal proteases assessed were expressed in maturation-stage enamel organ. Identified proteases included cathepsins B, D, F, H, K, L, O, S and Z. Tripeptidyl peptidases I and II as well as dipeptidyl peptidases I, II, III and IV were also found to be expressed. Immunohistochemical staining confirmed that the maturation-stage ameloblasts express cathepsins L and S and tripeptidyl peptidase II. Our results suggest that the ameloblasts are enriched by a large number of lysosomal proteases at maturation that are likely involved in the degradation of the organic matrix.
authors.affiliation: Department of Cytokine Biology, Forsyth Institute, Boston, Mass. 02115, USA.
authors.forename: Coralee E
authors.initials: CE
authors.lastname: Tye
authors.forename: Rachel L
authors.initials: RL
authors.lastname: Lorenz
authors.forename: John D
authors.initials: JD
authors.lastname: Bartlett
mesh_terms: D000565:Ameloblasts / Q000166:cytology / Q000201:enzymology; D000818:Animals; D002454:Cell Differentiation; D004658:Enamel Organ / Q000166:cytology / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D008247:Lysosomes / Q000201:enzymology*; D051379:Mice; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D010447:Peptide Hydrolases