title: Evaluation of antibacterial effects of a combination of Coptidis Rhizoma, Mume Fructus, and Schizandrae Fructus against Salmonella.
abstract: The antibacterial potential of a preparation of medicinal herbs for the treatment of salmonellosis was evaluated. The preparation contained a combination of methanolic extracts from Mume Fructus, Coptidis Rhizoma and Schizandrae Fructus, and is designated NP532. NP532 was tested against 26 different Salmonella stains. All these strains were affected by NP532 even though many of the salmonella strains were resistant to multiple antibiotics. The MIC values of this combination against the Salmonella strains varied from 0.49 to 7.8 mg/ml. The in vivo antibacterial activity of NP532 was examined using a S. Gallinarum infection chicken model. The chickens were initially infected with S. Gallinarum, and NP532 was then administered. This preparation was found to have major effects on mortality. The histopathological observations of the NP532 treated animals did not show any of the clinical signs and rarely showed histological damage associated with the disease. On the other hand, the untreated controls showed the clinical signs, e.g. congestion and necrotic changes in the liver, kidney, and spleen. This suggests that NP532 might be an effective treatment for salmonellosis.
authors.affiliation: College of Veterinary Medicine, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
authors.forename: Hyun Ae
authors.initials: HA
authors.lastname: Kwon
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Kwon
authors.forename: Dong-Yeul
authors.initials: DY
authors.lastname: Kwon
authors.forename: John Hwa
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001681:Biological Assay; D002645:Chickens; D015169:Colony Count, Microbial; D000089042:Coptis chinensis; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D012475:Salmonella / Q000187:drug effects* / Q000254:growth & development; D012478:Salmonella Food Poisoning / Q000188:drug therapy*; D032141:Schisandra / Q000737:chemistry; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C107393:Coptidis rhizoma extract