Compartmentalization of aquaporins in the human intestine.
Improper localization of water channel proteins called aquaporins (AQP) induce mucosal injury which is implicated in Crohn's disease and ulcerative colitis. The amino acid sequences of AQP3 and AQP10 are 79% similar and belong to the mammalian aquaglyceroporin subfamily. AQP10 is localized on the apical compartment of the intestinal epithelium called the glycocalyx while AQP3 is selectively targeted to the basolateral membrane. Despite the high sequence similarity and evolutionary relatedness, the molecular mechanism involved in the polarity, selective targeting and function of AQP3 and AQP10 in the intestine is largely unknown. Our hypothesis is that the differential polarity and selective targeting of AQP3 and AQP10 in the intestinal epithelial cells is influenced by amino acid signal motifs. We performed sequence and structural alignments to determine differences in signals for localization and posttranslational glycosylation. The basolateral sorting motif "YRLL" is present in AQP3 but absent in AQP10; while Nglycosylation signals are present in AQP10 but absent in AQP3. Furthermore, the C-terminal region of AQP3 is longer compared to AQP10. The sequence and structural differences between AQP3 and AQP10 provide insights into the differential compartmentalization and function of these two aquaporins commonly expressed in human intestines.
Cohly
Hari H P
HH
Department of Biology, Jackson State University, Jackson, Mississippi 39217, USA. hari.cohly@jsums.edu
Isokpehi
Raphael
R
Rajnarayanan
Rajendram V
RV
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D051400:Aquaporin 3 / Q000737:chemistry / Q000378:metabolism*; D020346:Aquaporins / Q000737:chemistry / Q000378:metabolism*; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism*; D016764:Cell Polarity; D003092:Colitis; D006031:Glycosylation; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000502:physiology*; D021381:Protein Transport; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
C438667:AQP10 protein, human; C496027:AQP3 protein, human; D020346:Aquaporins; D021382:Protein Sorting Signals; D051400:Aquaporin 3