title: [Effect of acupuncture on target action of ligustrazine in treatment of toxic deafness induced by gentamicin in guinea pigs].
abstract: OBJECTIVE
  To explore an effective method for treating Gentamicin-induced deafness and the mechanism.
  
  
  METHODS
  Guinea pigs were randomly divided into 5 groups: normal control group (group A), model group (group B), Ligustrazine group (group C), acupuncture group (group D) and Ligustrazine plus acupuncture group (group E). The group C, D and E were treated respectively by simple Ligustrazine, simple acupuncture at "Tinggong" (SI 19), "Yifeng" (TE 17), and "Waiguan" (TE 5), and Ligustrazine plus acupuncture. Ten days later, the auditory brainstem response (ABR) thresholds for the wave III , apoptosis and expressions of Bcl-2 and Bax proteins in the organ of Corti of the guinea pig were detected.
  
  
  RESULTS
  In the group E, the ABR threshold was significantly lower than that in the group C (P<0.05), and apoptotic cells, the expression of Bax protein and the ratio of Bax/Bcl-2 were lower than those in the group C and D in the organ of Corti, and Bcl-2 protein expression was increased.
  
  
  CONCLUSIONS
  Acupuncture at "Tinggong" (SI 19), "Yifeng" (TE 17), and "Waiguan" (TE 5) has a certain target-synergistic action on Ligustrazine and can increase therapeutic effect of Ligustrazine on Gentamicin-induced deafness, which are possible related with the inhibition of apoptosis, down-regulation of Bax expression and up-regulation of Bcl-2 expression.
authors.affiliation: Deafness Rehabilitation Institute, Zhejiang Chinese Medical University, Hangzhou, China. hpzheng04@yahoo.com.cn
authors.forename: Hua-Ping
authors.initials: HP
authors.lastname: Zheng
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Quan-Zhen
authors.initials: QZ
authors.lastname: Xu
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Liu
authors.forename: Bing-hua
authors.initials: BH
authors.lastname: Fan
mesh_terms: D015670:Acupuncture Therapy; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D003638:Deafness / Q000139:chemically induced / Q000628:therapy*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects; D005260:Female; D005839:Gentamicins / Q000633:toxicity*; D006168:Guinea Pigs; D008297:Male; D011719:Pyrazines / Q000627:therapeutic use*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D011719:Pyrazines; C017953:tetramethylpyrazine