title: Simultaneous determination of triazolam and its metabolites in human plasma by liquid chromatography-tandem mass spectrometry.
abstract: A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of triazolam and its metabolites, alpha-hydroxytriazolam (alpha-OHTRZ) and 4-hydroxytriazolam (4-OHTRZ), was developed and validated. Triazolam-D4 was used as the internal standard (IS). This analysis was carried out on a Thermo C(18) column and the mobile phase was composed of acetonitrile:H(2)O:formic acid (35:65:0.2, v/v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer using positive ion mode electrospray ionization (ESI) and quantification was performed by multiple reaction monitoring (MRM) mode. The MS/MS ion transitions monitored were m/z 343.1-->308.3, 359.0-->308.3, 359.0-->111.2 and 347.0-->312.0 for triazolam, alpha-OHTRZ, 4-OHTRZ and triazolam-D4, respectively. LLOQ of the analytical method was 0.05 ng/mL for triazolam and 0.1 ng/mL for alpha-OHTRZ and 4-OHTRZ. The within- and between-run precisions were less than 15.26% and accuracy was -8.08% to 13.33%. The method proved to be accurate and specific, and was applied to the pharmacokinetic study of triazolam in healthy Chinese volunteers.
authors.affiliation: Department of Disaster Management, Taiwan Police College, Taipei, Taiwan, ROC.
authors.forename: Ryh-Nan
authors.initials: RN
authors.lastname: Pan
authors.forename: Chang-Ching
authors.initials: CC
authors.lastname: Lin
authors.forename: Pei-Wei
authors.initials: PW
authors.lastname: Huang
authors.forename: Cheng-Huei
authors.initials: CH
authors.lastname: Hsiong
authors.forename: Li-Heng
authors.initials: LH
authors.lastname: Pao
mesh_terms: D014151:Anti-Anxiety Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014229:Triazolam / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D014151:Anti-Anxiety Agents; D014229:Triazolam