Paternity determination of captive bottlenose dolphins (Tursiops truncatus) using microsatellite DNA analysis.
We applied previously published PCR primer pairs to amplify alleles at three polymorphic microsatellite loci to determine the genetic relationship of 6 bottlenose dolphins (Tursiops truncatus) that were living together in a Japanese aquarium. The three microsatellite loci were sufficient to determine the haplotype relationships of the six dolphins, which represented three different generations. It was confirmed that this genotyping method is simple and economical for assessing, establishing and maintaining genetic diversity in captive populations and will become a very effective technique for ex situ conservation in aquariums and zoos.
Sumiyama
Daisuke
D
Department of Molecular Life Science, School of Medicine, Tokai University, Bouseidai, Kanagawa, Japan.
Kitamura
Shouichi
S
Terasawa
Fumio
F
Hori
Yukiko
Y
Murata
Koichi
K
Kulski
Jerzy K
JK
Inoko
Hidetoshi
H
D000818:Animals; D050681:Bottle-Nosed Dolphin / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000662:veterinary; D005260:Female; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D010334:Paternity; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000662:veterinary
D004247:DNA