title: Fluoroquinolone enhances the mutation frequency for meropenem-selected carbapenem resistance in Pseudomonas aeruginosa, but use of the high-potency drug doripenem inhibits mutant formation.
abstract: The mutation frequency for carbapenem resistance in Pseudomonas aeruginosa strains that were selected with carbapenems was enhanced in the presence of subinhibitory concentrations of fluoroquinolones. The mutants showed either a loss of OprD activity or increased mexAB-oprM expression. The highest mutant isolation frequency was obtained by selection with meropenem, while doripenem inhibited mutant growth.
authors.affiliation: Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanimoto
authors.forename: Haruyoshi
authors.initials: H
authors.lastname: Tomita
authors.forename: Shuhei
authors.initials: S
authors.lastname: Fujimoto
authors.forename: Katsuko
authors.initials: K
authors.lastname: Okuzumi
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Ike
mesh_terms: D001483:Base Sequence; D015780:Carbapenems / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D000077726:Doripenem; D024841:Fluoroquinolones / Q000494:pharmacology*; D005798:Genes, Bacterial / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D000077731:Meropenem; D009154:Mutation / Q000187:drug effects*; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D013845:Thienamycins / Q000494:pharmacology*; D018440:beta-Lactam Resistance / Q000235:genetics*
chemical_list: D015780:Carbapenems; D017931:DNA Primers; D004269:DNA, Bacterial; D024841:Fluoroquinolones; D013845:Thienamycins; D000077726:Doripenem; D000077731:Meropenem