A sensitive human bone assay for quantitation of tigecycline using LC/MS/MS.
Tigecycline (Tygacil,Wyeth) is a first-in-class, broad spectrum antibiotic with activity against multiple-resistant organisms. In order to address the unexpectedly low tigecycline concentrations in human bone samples analyzed using a LC/MS/MS method developed elsewhere, we have developed and validated a new and sensitive human bone assay for the quantitation of tigecycline using LC/MS/MS. The new method utilizes the addition of a stabilizing agent to the human bone sample, homogenization of human bone in a strong acidic-methanol extraction solvent, centrifugation of the bone suspension, separation by liquid chromatography, and detection of tigecycline by mass spectrometry. Linearity was demonstrated over the concentration range from 50 to 20,000 ng/g using a 0.1g human bone sample. The intra- and inter-day accuracy of the assay was within 100+/-15%, and the corresponding precision (CV) was <15%. The stability of tigecycline was evaluated and shown to be acceptable under the assay conditions. The extraction recovery of tigecycline with the current method was 79% when using radio-labeled rat bone samples as a substitute for human bone samples. Twenty-four human bone samples collected previously from volunteers without infections who had elective orthopedic surgery after receiving a single dose of tigecycline were re-analyzed using the current validated method. Tigecycline concentrations in these samples ranged from 238 to 794 ng/g with a mean value 9 times higher than the mean concentration previously reported. The data demonstrated that the current method has significantly higher extraction efficiency than the previously reported method.
Ji
Allena J
AJ
Bioanalytical R & D, Wyeth Research, 401 North Middletown Road, Pearl River, NY 10965, USA. Jia@wyeth.com
Saunders
James P
JP
Amorusi
Peter
P
Wadgaonkar
Nandan D
ND
O'Leary
Kenneth
K
Leal
Mauricio
M
Dukart
Gary
G
Marshall
Bonnie
B
Fluhler
Eric N
EN
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D001681:Biological Assay; D001842:Bone and Bones / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008911:Minocycline / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D011786:Quality Control; D016032:Randomized Controlled Trials as Topic; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000078304:Tigecycline
D000900:Anti-Bacterial Agents; D012997:Solvents; D000078304:Tigecycline; D008911:Minocycline