Hydrolysis of adenine nucleotides in platelets from patients with acute myocardial infarction.
OBJECTIVE
To investigate the rate of ATP, ADP and AMP hydrolysis on the surface of platelets from acute myocardial infarction (AMI) patients.


METHODS
Twenty-five patients diagnosed with AMI, through clinical criteria, electrocardiographic changes and increase of cardiac biomarkers, as well as 25 healthy patients were selected. The hydrolysis of ATP, ADP and AMP was verified in isolated platelets of these patients.


RESULTS
The results demonstrated that an increase in ATP (54%) and ADP (45%) hydrolysis occurred in AMI patients when compared to the control group. The hydrolysis of AMP also increased by 46% in AMI patients probably leading to an enhancement in the adenosine level.


CONCLUSIONS
Our results suggest an increase in nucleotide hydrolysis in platelets from AMI patients, which could be related to a compensatory organic response to thrombotic events that occur in AMI.
Bagatini
Margarete D
MD
Departamento de Química, Centro de Ciências Naturais e Exatas, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.
Martins
Caroline C
CC
Battisti
Vanessa
V
Spanevello
Roselia M
RM
Gasparetto
Diogo
D
Rosa
Cintia S
CS
Gonçalves
Jamile Fabrin
JF
Schetinger
Maria Rosa C
MR
dos Santos
Romualdo B
RB
Morsch
Vera Maria
VM
D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001241:Aspirin / Q000494:pharmacology / Q000627:therapeutic use; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D000077144:Clopidogrel; D005260:Female; D006801:Humans; D006868:Hydrolysis / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D013988:Ticlopidine / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use
D000227:Adenine Nucleotides; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000077144:Clopidogrel; D013988:Ticlopidine; D001241:Aspirin