Nuclear deformation and expression change of cartilaginous genes during in vitro expansion of chondrocytes.
Cartilaginous gene expression decreased when chondrocytes were expanded on cell-culture plates. Understanding the dedifferentiation mechanism may provide valuable insight into cartilage tissue engineering. Here, we demonstrated the relationship between the nuclear shape and gene expression during in vitro expansion culture of chondrocytes. Specifically, the projected nuclear area increased and cartilaginous gene expressions decreased during in vitro expansion culture. When the nuclear deformation was recovered by cytochalasin D treatment, aggrecan expression was up-regulated and type I collagen (Col1a2) expression was down-regulated. These results suggest that nuclear deformation may be one of the mechanisms for chondrocyte dedifferentiation during in vitro expansion culture.
Hoshiba
Takashi
T
Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044, Japan.
Yamada
Tomoe
T
Lu
Hongxu
H
Kawazoe
Naoki
N
Tateishi
Tetsuya
T
Chen
Guoping
G
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D003094:Collagen / Q000235:genetics; D024042:Collagen Type I; D015638:Cytochalasin D / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D010641:Phenotype; D023822:Tissue Engineering
D024042:Collagen Type I; D015638:Cytochalasin D; D003094:Collagen