title: Biocompatibility and bone mineralization potential of 45S5 Bioglass-derived glass-ceramic scaffolds in chick embryos.
abstract: The aim of the present study was to evaluate the biocompatibility and bone mineralization potential of 45S5 Bioglass-derived glass-ceramic scaffolds using a chick embryo shell-less (ex ovo) culture system. Chick embryos were divided into two groups: control (C) and experimental (E). Scaffolds were placed on the chorioallantoic membrane (CAM) in embryos of group E at 10 days of total incubation. The 45S5 Bioglass-derived glass-ceramic scaffolds proved to be biocompatible in terms of the absence of inflammatory response at the implant site (CAM). Moreover, no alterations in the other end-points assessed, i.e. survival, stage of embryonic development and body weight, were detected. However, body length was greater in group E embryos than in group C embryos (p0.05). A marked reduction (93%) in Ca content in the scaffolds was evidenced by energy-dispersive X-ray analysis at 5 days post-implantation. Calcium release from the scaffold implanted on the CAM might have been responsible for the restoration of the bone-like phenotype in chick embryonic skeleton of group E as detected by Alcian blue-Alizarin red double staining, as well as by histological and microchemical analyses. Conversely, the control embryos exhibited a chondrogenic phenotype.
authors.affiliation: Research Laboratory, National Atomic Energy Commission (CNEA-Reg. Noroeste), Salta A4408FTV, Argentina.
authors.forename: Gabriela E
authors.initials: GE
authors.lastname: Vargas
authors.forename: Rosa Vera
authors.initials: RV
authors.lastname: Mesones
authors.forename: Oana
authors.initials: O
authors.lastname: Bretcanu
authors.forename: José M Porto
authors.initials: JM
authors.lastname: López
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
authors.forename: Alejandro
authors.initials: A
authors.lastname: Gorustovich
mesh_terms: D000423:Alcian Blue / Q000737:chemistry; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002516:Ceramics / Q000737:chemistry*; D002642:Chick Embryo; D007249:Inflammation; D007477:Ions; D008422:Materials Testing; D010641:Phenotype; D023822:Tissue Engineering / Q000379:methods; D014965:X-Rays
chemical_list: D000880:Anthraquinones; D001672:Biocompatible Materials; C022107:Bioglass; D018786:Bone Substitutes; D007477:Ions; C010078:alizarin; D000423:Alcian Blue; D002118:Calcium