title: Clonality and HPV infection analysis of concurrent glandular and squamous lesions and adenosquamous carcinomas of the uterine cervix.
abstract: We analyzed the clonality and human papillomavirus (HPV) infection status of concurrent glandular and squamous lesions and adenosquamous carcinomas of the uterine cervix to clarify their histogenesis. The glandular and squamous components were clonally different from each other in 7 informative concurrent lesions. HPV was episomal in 2 polyclonal glandular dysplasias (GDs). HPV was in a mixed integrated-episomal form in a monoclonal GD, an adenocarcinoma in situ, and an adenocarcinoma. Both tumor components were monoclonal in origin in 6 adenosquamous carcinomas, with identical patterns of X-chromosomal inactivation and types and physical status of HPV. These results imply that the concurrent glandular and squamous lesions are formed separately, whereas adenosquamous carcinoma is more likely to be a combination tumor of monoclonal origin, and that integration of HPV has an important role in the progression from polyclonal GD through monoclonal expansion to adenocarcinoma in situ and adenocarcinoma.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ueda
authors.forename: Takashi
authors.initials: T
authors.lastname: Miyatake
authors.forename: Mika
authors.initials: M
authors.lastname: Okazawa
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kimura
authors.forename: Takahito
authors.initials: T
authors.lastname: Miyake
authors.forename: Kazuko
authors.initials: K
authors.lastname: Fujiwara
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yoshino
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Nakashima
authors.forename: Masami
authors.initials: M
authors.lastname: Fujita
authors.forename: Takayuki
authors.initials: T
authors.lastname: Enomoto
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology / Q000821:virology*; D000914:Antibodies, Viral / Q000032:analysis; D018196:Carcinoma, Adenosquamous / Q000276:immunology / Q000821:virology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology / Q000821:virology*; D002584:Cervix Uteri / Q000276:immunology / Q000473:pathology* / Q000821:virology*; D016889:Endometrial Neoplasms / Q000276:immunology / Q000821:virology*; D005260:Female; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D006801:Humans; D030361:Papillomavirus Infections / Q000276:immunology / Q000473:pathology*; D016133:Polymerase Chain Reaction; D002578:Uterine Cervical Dysplasia / Q000276:immunology / Q000473:pathology
chemical_list: D000914:Antibodies, Viral