title: Enhancement of recombinant hemoglobin production in Escherichia coli BL21(DE3) containing the Plesiomonas shigelloides heme transport system.
abstract: To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) grown in medium containing heme.
authors.affiliation: Department of Biology, University of Texas of the Permian Basin, 4901 East University Blvd., Odessa, TX 79762-0001, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Villarreal
authors.forename: C L
authors.initials: CL
authors.lastname: Phillips
authors.forename: A M
authors.initials: AM
authors.lastname: Kelley
authors.forename: S
authors.initials: S
authors.lastname: Villarreal
authors.forename: A
authors.initials: A
authors.lastname: Villaloboz
authors.forename: P
authors.initials: P
authors.lastname: Hernandez
authors.forename: J S
authors.initials: JS
authors.lastname: Olson
authors.forename: D P
authors.initials: DP
authors.lastname: Henderson
mesh_terms: D001692:Biological Transport / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics; D005798:Genes, Bacterial; D024861:Genetic Enhancement; D006418:Heme / Q000378:metabolism; D006454:Hemoglobins / Q000096:biosynthesis*; D006801:Humans; D010957:Plasmids; D016981:Plesiomonas / Q000235:genetics*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D006454:Hemoglobins; D011994:Recombinant Proteins; D006418:Heme