Curli expression by Escherichia coli strains isolated from bovine mastitis.
The aim of the study was to determine the expression of mannose-sensitive and mannose-resistant adhesins by agglutination of cattle, sheep, goat, rabbit, horse, and chicken red blood cell assay, and curli fimbriae by Congo red binding assay among 341 E. coli strains isolated from 51 milk samples of clinically recognized bovine mastitis. Curli fimbriae expression within biofilms created on an inert surface was also investigated. To determine whether curli fimbriae are expressed both in conditions optimal for their production and in conditions resembling the host organism, the study was conducted in anaerobic atmosphere at 37 degrees C, and at room temperature in aerobic atmosphere. The results demonstrated that although the E. coli isolates examined were deprived of mannose-sensitive and mannose-resistant adhesins they were able to produce curli fimbriae in both aerobic and anaerobic conditions at room and higher temperature, indicating that these adhesins may be involved in the pathogenesis of bovine mastitis.
Karczmarczyk
A
A
Department and Clinic of Obstetrics Ruminant Diseases and Animal Health, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Twardoń
J
J
Sobieszczańska
B
B
Pajaczkowska
M
M
D018830:Adhesins, Escherichia coli / Q000378:metabolism*; D000818:Animals; D018441:Biofilms / Q000254:growth & development*; D002417:Cattle; D002478:Cells, Cultured; D003224:Congo Red; D004912:Erythrocytes / Q000382:microbiology; D004926:Escherichia coli / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008414:Mastitis, Bovine / Q000382:microbiology*
D018830:Adhesins, Escherichia coli; D003224:Congo Red