title: Liposome electrokinetic capillary chromatography in the study of analyte-phospholipid membrane interactions. Application to pesticides and related compounds.
abstract: Interactions between low-molar mass analytes and phospholipid membranes were studied by liposome electrokinetic capillary chromatography (LEKC). The analytes were pesticides, some degradation products, and compounds associated with the manufacture of pesticides. Negatively charged liposome dispersions with different zwitterionic lipids (PC) were applied to the determination of retention factors (k) of 15 charged and uncharged compounds. The liposome dispersions consisted of 80:20 mol% of 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC)/1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)/POPS, and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/POPS. Retention factors were calculated from the effective electrophoretic mobilities of the analytes under LEKC and CZE conditions and from the effective electrophoretic mobilities of the liposomes, determined by CZE with a polyacrylamide-coated capillary. Determining the liposome mobilities in this way proved to be a good alternative to the conventional method employing a liposome marker compound. The log k values of the analytes for the different liposome dispersed phases were correlated with one another. In addition, correlation curves were determined between log k and calculated octanol-water partition coefficients. The results showed that the zwitterionic phospholipid in the liposome has a major impact on the interactions between the tested compounds and the lipid membranes.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland. susanne.wiedmer@helsinki.fi
authors.forename: Susanne K
authors.initials: SK
authors.lastname: Wiedmer
authors.forename: Pipsa
authors.initials: P
authors.lastname: Kulovesi
authors.forename: Marja-Liisa
authors.initials: ML
authors.lastname: Riekkola
mesh_terms: D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D007700:Kinetics; D008051:Lipid Bilayers / Q000032:analysis*; D008081:Liposomes / Q000737:chemistry*; D015394:Molecular Structure; D010575:Pesticides / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D010575:Pesticides; D010743:Phospholipids