Comparative effect of overexpressed phaJ and fabG genes supplementing (R)-3-hydroxyalkanoate monomer units on biosynthesis of mcl-polyhydroxyalkanoate in Pseudomonas putida KCTC1639.
phaJ and fabG genes encoding (R)-specific 2-enoyl-CoA hydratase and 3-ketoacyl-acyl carrier protein reductase, respectively, were cloned from Pseudomonas putida KCTC1639 and amplified in the parent strain to achieve oversupplementation of (R)-3-hydroxyalkanoate monomer units for the biosynthesis of medium-chain-length polyhydroxyalkanoate (mcl-PHA). The comparative effect of the overexpressed fabG and phaJ genes in P. putida KCTC1639 on the biosynthesis of mcl-PHA and the cell growth were elucidated. Overexpression of phaJ enhanced the biosynthesis of mcl-PHA, increasing its content and concentration from 18% to 27% and 0.38 to 0.51 g/l, respectively. Conversely, fabG overexpression tended to depress the biosynthesis of mcl-PHA, possibly due to the reversible conversion of (R)-3-hydroxyalkanoate monomer units into 3-ketoacyl-CoA.
Vo
Minh Tri
MT
Department of Genetic Engineering, College of Natural Sciences Kyungpook National University, Daegu 702-701, South Korea.
Lee
Kwang-Woo
KW
Jung
Young-Mi
YM
Lee
Yong-Hyun
YH
D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Q000378:metabolism*; D000429:Alcohol Oxidoreductases; D004746:Enoyl-CoA Hydratase / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D015854:Up-Regulation
D054813:Polyhydroxyalkanoates; D000429:Alcohol Oxidoreductases; C023744:acetoacetyl-CoA reductase; C077402:3-ketoacyl-acyl carrier protein synthase III; D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; D004746:Enoyl-CoA Hydratase; C083735:long-chain-enoyl-CoA hydratase