Regulation of the friction coefficient of articular cartilage by TGF-beta1 and IL-1beta.
Articular cartilage functions to provide a low-friction surface for joint movement for many decades of life. Superficial zone protein (SZP) is a glycoprotein secreted by chondrocytes in the superficial layer of articular cartilage that contributes to effective boundary lubrication. In both cell and explant cultures, TGF-beta1 and IL-1beta have been demonstrated to, respectively, upregulate and downregulate SZP protein levels. It was hypothesized that the friction coefficient of articular cartilage could also be modulated by these cytokines through SZP regulation. The friction coefficient between cartilage explants (both untreated and treated with TGF-beta1 or IL-1beta) and a smooth glass surface due to sliding in the boundary lubrication regime was measured with a pin-on-disk tribometer. SZP was quantified using an enzyme-linked immunosorbant assay and localized by immunohistochemistry. Both TGF-beta1 and IL-1beta treatments resulted in the decrease of the friction coefficient of articular cartilage in a location- and time-dependent manner. Changes in the friction coefficient due to the TGF-beta1 treatment corresponded to increased depth of SZP staining within the superficial zone, while friction coefficient changes due to the IL-1beta treatment were independent of SZP depth of staining. However, the changes induced by the IL-1beta treatment corresponded to changes in surface roughness, determined from the analysis of surface images obtained with an atomic force microscope. These findings demonstrate that the low friction of articular cartilage can be modified by TGF-beta1 and IL-1beta treatment and that the friction coefficient depends on multiple factors, including SZP localization and surface roughness.
DuRaine
Grayson
G
Center for Tissue Regeneration and Repair, Department of Orthopaedic Surgery, University of California, Davis, Medical Center, Sacramento, CA 95817, USA.
Neu
Corey P
CP
Chan
Stephanie M T
SM
Komvopoulos
Kyriakos
K
June
Ronald K
RK
Reddi
A Hari
AH
D000818:Animals; D001696:Biomechanical Phenomena; D002358:Cartilage, Articular / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005269:Femur / Q000502:physiology; D017276:Friction / Q000187:drug effects / Q000502:physiology*; D006023:Glycoproteins / Q000378:metabolism; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000494:pharmacology / Q000502:physiology*; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D011509:Proteoglycans / Q000378:metabolism; D046509:Tissue Culture Techniques; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology / Q000502:physiology*; D000099158:Proteoglycan 4
D006023:Glycoproteins; D053583:Interleukin-1beta; D011509:Proteoglycans; D053773:Transforming Growth Factor beta1; D000099158:Proteoglycan 4; C498836:PRG4 protein, Bos taurus