title: The effect of premixed schedule on the crystal formation of calcium phosphate cement-chitosan composite with added tetracycline.
abstract: In this study, calcium phosphate cements (CPC) were prepared by mixing cement powders of tetracalcium phosphate (TTCP) with a cement liquid of phosphate acid saline solution. Tetracycline (TTC)-CPC, chitosan-CPC and chitosan-TTC-CPC were investigated with different premixed schedule. It was demonstrate that both TTC and chitosan worked on the phase transition and crystal characteristics. TTCP mixed with phosphate acid saline solution had similar features of Fourier transform-infrared spectrometry (FT-IR) no matter it was mixed with chitosan or TTC or both. TTC premixed with cement liquid or powder had significant different features of FT-IR and 876 cm(-1) seemed to be a special peak for TTC when TTC was premixed with cement liquid. This was also supported by XRD analysis, which showed that TTC premixed with cement liquid improved phase transition of TTCP to OCP. Chitosan, as organic additive, regulates the regular crystal formation and inhibits the phase transition of TTCP to OCP, except when it is mingled with cement liquid premixed with TTC in field scanning electron microscope. It was concluded that the premixed schedule influences the crystal formation and phase transition, which may be associated with its biocompatibility and bioactivities in vivo.
authors.affiliation: Department of Stomatology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China. maojing1999@yahoo.com
authors.forename: Jing
authors.initials: J
authors.lastname: Mao
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Liyun
authors.initials: L
authors.lastname: Yao
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001843:Bone Cements / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003738:Dental Cements / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D013752:Tetracycline / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; D003738:Dental Cements; D010710:Phosphates; C485830:tetracalcium phosphate; D048271:Chitosan; C020243:calcium phosphate; D013752:Tetracycline