Methods for the production of helper-dependent adenoviral vectors.
Helper-dependent adenoviral vectors (HDAd) are deleted of all viral coding sequences and have demonstrated tremendous potential for gene therapy by providing long-term, high-level transgene expression in the absence of chronic toxicity. Thus, HDAd are superior to early generation Ad for gene therapy of genetic diseases where long-term transgene expression is required. This chapter describes in detail the rescue, amplification, and large-scale production of HDAd.
Palmer
Donna J
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Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
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Philip
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D000256:Adenoviridae / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D002452:Cell Count; D002460:Cell Line; D005822:Genetic Vectors / Q000096:biosynthesis* / Q000302:isolation & purification; D006378:Helper Viruses / Q000378:metabolism*; D006801:Humans; D019426:Integrases / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D014779:Virus Replication
C045073:Cre recombinase; D019426:Integrases