title: Caenorhabditis elegans mutant allele identification by whole-genome sequencing.
abstract: Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University Medical Center, New York, New York 10032, USA.
authors.forename: Sumeet
authors.initials: S
authors.lastname: Sarin
authors.forename: Snehit
authors.initials: S
authors.lastname: Prabhu
authors.forename: M Maggie
authors.initials: MM
authors.lastname: O'Meara
authors.forename: Itsik
authors.initials: I
authors.lastname: Pe'er
authors.forename: Oliver
authors.initials: O
authors.lastname: Hobert
mesh_terms: D000483:Alleles; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D018509:DNA, Helminth / Q000235:genetics; D049751:Genome, Helminth; D009154:Mutation; D020641:Polymorphism, Single Nucleotide
chemical_list: D018509:DNA, Helminth