title: Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana.
abstract: We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.
authors.affiliation: ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Western Australia, Australia.
authors.forename: Chris
authors.initials: C
authors.lastname: Carrie
authors.forename: Monika W
authors.initials: MW
authors.lastname: Murcha
authors.forename: Kristina
authors.initials: K
authors.lastname: Kuehn
authors.forename: Owen
authors.initials: O
authors.lastname: Duncan
authors.forename: Michelle
authors.initials: M
authors.lastname: Barthet
authors.forename: Penelope M
authors.initials: PM
authors.lastname: Smith
authors.forename: Holger
authors.initials: H
authors.lastname: Eubel
authors.forename: Etienne
authors.initials: E
authors.lastname: Meyer
authors.forename: David A
authors.initials: DA
authors.lastname: Day
authors.forename: A Harvey
authors.initials: AH
authors.lastname: Millar
authors.forename: James
authors.initials: J
authors.lastname: Whelan
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D008928:Mitochondria / Q000201:enzymology*; D009252:NADPH Dehydrogenase / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020675:Peroxisomes / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000378:metabolism*; D020539:Sequence Analysis, Protein
chemical_list: D010940:Plant Proteins; D049452:Green Fluorescent Proteins; D009252:NADPH Dehydrogenase