Biohydrogen production from cattle wastewater by enriched anaerobic mixed consortia: influence of fermentation temperature and pH.
Experiments were conducted to select a natural mixed microflora seed source and investigate the effect of temperature and pH on fermentative hydrogen (H2) production from cattle wastewater by sewage sludge. Sewage sludge was shown to have higher cumulative H2 production than other inoculum collected from cow dung compost, chicken manure compost, and river sludge. Experimental results show that H2 production from cattle wastewater was significantly affected by both pH and temperature of the culture. The maximum H2 yield was obtained at pH 5.5. H2 yield and the ratio of butyrate/acetate (Bu/Ac) followed a similar production trend, suggesting that butyrate formation might favor H2 production. The optimal temperature for H2 production from cattle wastewater was 45 degrees C with peak values of H2 production (368 ml), hydrogen yield of 319 ml H2/g chemical oxygen demand (COD) consumed, and butyrate/acetate ratio of 1.43. Presence of ethanol and propionic acid indicated decreased hydrogen production; their concentrations were also affected by pH and temperature. A modified Gompertz model adequately described H2 production and bacterial growth.
Tang
Gui-Lan
GL
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100094, PR China.
Huang
Jian
J
Sun
Zhen-Jun
ZJ
Tang
Qin-Qing
QQ
Yan
Chun-Hao
CH
Liu
Guang-Qing
GQ
D000818:Animals; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D001711:Biotransformation; D002417:Cattle / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D018920:Coculture Techniques; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000517:prevention & control*; D013696:Temperature; D014873:Water Pollutants / Q000378:metabolism*; D018508:Water Purification / Q000379:methods
D007220:Industrial Waste; D014873:Water Pollutants; D006859:Hydrogen