title: Study of oxidative damage in growing-finishing pigs with continuous excess dietary chromium picolinate intake.
abstract: Chromium picolinate (CrPic) is a popular nutritional supplement; however, its safety has been questioned as it may be a source of oxidative stress that induces genotoxicity. The current work investigated the effect of excessive CrPic intake on oxidative damage in growing-finishing pigs. Thirty castrated male pigs, weighing approximately 30 kg each, were randomly divided into five groups and fed a diet with 0, 200, 800, 1,600, 3,200 microg of Cr/kg feed as CrPic for 80 days (approximately the entire growing-finishing period). High CrPic dose significantly decreased superoxide dismutase activity in serum at 80 days as well as the catalase activity in kidney (p < 0.05); however, compared to controls, malondialdehyde in tissue and serum, urinary 8-hydroxydeoxyguanosine level, and DNA strand breaks in liver and kidney had no notable differences (p > 0.05). These results suggested that long-term exposure to different doses of CrPic in feed did not increase the formation of biomarkers of oxidative damage in growing-finishing pigs. However, taking into account the changes of antioxidant enzymes activity, excessive dietary CrPic intake was not recommended in this study.
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100094, People's Republic of China.
authors.forename: Gao-Yi
authors.initials: GY
authors.lastname: Tan
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Zheng
authors.forename: Min-Hong
authors.initials: MH
authors.lastname: Zhang
authors.forename: Jing-Hai
authors.initials: JH
authors.lastname: Feng
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Bi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D000818:Animals; D002374:Catalase / Q000378:metabolism; D019587:Dietary Supplements / Q000633:toxicity*; D006147:Guanine / Q000031:analogs & derivatives / Q000652:urine; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010848:Picolinic Acids / Q000008:administration & dosage / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000097:blood; D013552:Swine
chemical_list: D010848:Picolinic Acids; D008315:Malondialdehyde; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002374:Catalase; D013482:Superoxide Dismutase; C030614:picolinic acid