title: Knockout animals and natural mutations as experimental and diagnostic tool for studying tight junction functions in vivo.
abstract: Two sides of functions of tight junctions; the barrier and the channel in the paracellular pathway are believed to be essential for the development and physiological functions of organs. Recent identification of molecular components of tight junctions has enabled us to analyze their functions by generating knockout mice of the corresponding genes. In addition, positional cloning has identified mutations in the genes of several components of tight junctions in hereditary diseases. These studies have highlighted in vivo functions of tight junctions.
authors.affiliation: Department of Physiology and Cell Biology, Kobe University, Chuo-ku, Kobe 650-0017, Japan. furuse@med.kobe-u.ac.jp
authors.forename: Mikio
authors.initials: M
authors.lastname: Furuse
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D062445:Claudin-1; D062794:MARVEL Domain Containing 2 Protein; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D062793:Occludin; D010750:Phosphoproteins / Q000235:genetics; D019108:Tight Junctions / Q000235:genetics / Q000502:physiology*; D062825:Zonula Occludens Proteins; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein
chemical_list: D002352:Carrier Proteins; D062445:Claudin-1; C568113:Cldn1 protein, mouse; D062794:MARVEL Domain Containing 2 Protein; C508309:Marveld2 protein, mouse; D008565:Membrane Proteins; D062793:Occludin; C568986:Ocln protein, mouse; D010750:Phosphoproteins; C569043:Tjp1 protein, mouse; C569047:Tjp2 protein, mouse; C491774:Tjp3 protein, mouse; D062825:Zonula Occludens Proteins; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein