[Effect of shuanghuanglian injection on the activation and proliferation of T lymphocytes in mice in vitro].
OBJECTIVE
To study the effect of shuanghuanglian injection on the activation and proliferation of T lymphocytes in mice in vitro.


METHODS
The toxic effect of SHL on lymphocytes in mice was estimated by MTT test; Double-fluorescent plus flow cytometry to analyze the effect of SHL on the activation of T lymphocytes in mice stimulated by ConA in vitro; MTT test and CFDA-SE plus flow cytometry were used to detect the effect of SHL on the proliferation of T lymphocytes in mice induced by ConA in vitro.


RESULTS
SHL had little side effect on lymphocytes in mice in vitro; At the mass concentration of 60, 80, 100, 120 mg/L, SHL inhibited the activation of T lymphocytes in mice stimulated by ConA in vitro (P<0.01); Both MTT test and CFDA-SE stain sign that SHL inhibited the activation of T lymphocytes in mice induced by ConA in vitro at the mass concentration of 60, 80, 100, 120 mg/L (P<0.01).


CONCLUSIONS
SHL can inhibit the activation and proliferation of T lymphocytes in mice in vitro.
Yang
Zhi
Z
Laboratory of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China. yangzhi-198394@163.com
Zeng
Yao-Ying
YY
Huang
Xiu-Yan
XY
Teng
Fei
F
Li
Lin
L
Song
Bing
B
Yao
Man-Lin
ML
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D007155:Immunologic Factors / Q000008:administration & dosage / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D003208:Concanavalin A