Combination of two antibody fragments F(ab')(2)/Fab: an alternative for scorpion envenoming treatment.
Immunotherapy is the only effective treatment for scorpion stings. However the efficiency of this treatment varies depending on the forms of the antibodies and route of administration used. The antibodies are mostly injected as F(ab )(2) fragments. In this study, we investigated damage to the heart and lung tissue and the inflammatory response caused by Androctonus australis hector venom, its toxic fraction after molecular filtration or the isolated main alpha toxin (Aah II) in the presence or absence of different antibody molecules. A mixture of antibody fragments, F(ab )(2) and Fab, significantly reduced local leukocytosis, hemorrhage and inflammatory oedema induced by the A. australis hector venom and its toxins.
Sami-Merah
Sassia
S
Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, USTHB BP 32, El -Alia Bab Ezzouar, 16111, Algiers, Algeria; Laboratoire de Recherche et Développement sur les Venins, Institut Pasteur d'Algérie, Algiers, Algeria.
Hammoudi-Triki
Djelila
D
Martin-Eauclaire
Marie-France
MF
Laraba-Djebari
Fatima
F
D000818:Animals; D004195:Disease Models, Animal; D006335:Heart Injuries / Q000139:chemically induced / Q000628:therapy*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology / Q000627:therapeutic use*; D007167:Immunotherapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000628:therapy*; D012604:Scorpion Venoms / Q000008:administration & dosage* / Q000276:immunology; D012605:Scorpions / Q000737:chemistry*
D007140:Immunoglobulin Fab Fragments; D012604:Scorpion Venoms; C053969:scorpion toxin II, Androctonus