[Effect of NECL1 on the proliferation of T98G glioma cell line].
OBJECTIVE
To study the regulation role of tumor suppressor NECL1 on the proliferation of glioma cell line.


METHODS
We detected the expression level of NECL1 in human normal brain tissue and glioma cell lines using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot. T98G cell line in which NECL1 was silent and whose transfection efficiency was relatively high as target cell was chosen, and the effect of NECL1 on the proliferation of T98G cell line in vitro was detected by using cell growth curve, flow cytometry, and Hoechst staining.


RESULTS
NECL1 was abrogated or markedly reduced in 6 glioma cell lines. When NECL1 was overexpressed in T98G cell line, the cell growth rate obviously decreased and the number of apoptotic cells remarkably increased when compared with the control group.


CONCLUSIONS
NECL1 may inhibit the proliferation of T98G cells by inducing its apoptosis.
Gao
Jing
J
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Chen
Tao
T
Yin
Bin
B
Gong
Yan-Hua
YH
Qiang
Bo-Qin
BQ
Yuan
Jian-Gang
JG
Peng
Xiao-Zhong
XZ
D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D007136:Immunoglobulins; D008565:Membrane Proteins