title: T-cell responses to survivin in cancer patients undergoing radiation therapy.
abstract: OBJECTIVE
  The goal of this study was to determine if radiation therapy (RT) of human cancer enhances or diminishes tumor-specific T-cell reactivity. This is important if immunotherapy is to be harnessed to improve the outcome of cancer radiotherapy.
  
  
  METHODS
  Lymphocytes were isolated from colorectal cancer (CRC) patients before, during, and after presurgical chemoradiotherapy. Similar samples were taken from prostate cancer patients receiving standard RT. The level of CD8(+) T cells capable of binding tetramers for the tumor-associated antigen survivin, which is overexpressed in both cancer types, was enumerated in HLA-A*0201 patient samples. CD4(+), CD25(high), Foxp3(+) cells were also enumerated to evaluate therapy-induced changes in T(regulatory) cells. For CRC patients, most of whom were enrolled in a clinical trial, pathologic response data were available, as well as biopsy and resection specimens, which were stained for cytoplasmic and intranuclear survivin.
  
  
  RESULTS
  Survivin-specific CD8(+) T lymphocytes were detected in the peripheral blood of CRC and prostate cancer patients and increased after therapy in some, but not all, patients. Increases were more common in CRC patients whose tumor was downstaged after chemoradiotherapy. Biopsy specimens from this cohort generally had higher nuclear to cytoplasmic survivin expression. T(regulatory) cells generally increased in the circulation following therapy but only in CRC patients.
  
  
  CONCLUSIONS
  This study indicates that RT may increase the likelihood of some cancer patients responding to immunotherapy and lays a basis for future investigations aimed at combining radiation and immunotherapy.
authors.affiliation: Department of Radiation Oncology, University of California at Los Angeles, Los Angeles, California 90095-1714, USA.
authors.forename: Dörthe
authors.initials: D
authors.lastname: Schaue
authors.forename: Begonya
authors.initials: B
authors.lastname: Comin-Anduix
authors.forename: Antoni
authors.initials: A
authors.lastname: Ribas
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Lee
authors.initials: L
authors.lastname: Goodglick
authors.forename: James W
authors.initials: JW
authors.lastname: Sayre
authors.forename: Annelies
authors.initials: A
authors.lastname: Debucquoy
authors.forename: Karin
authors.initials: K
authors.lastname: Haustermans
authors.forename: William H
authors.initials: WH
authors.lastname: McBride
mesh_terms: D001706:Biopsy; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism*; D005260:Female; D051858:Forkhead Transcription Factors / Q000096:biosynthesis; D015234:HLA-A Antigens / Q000378:metabolism; D015789:HLA-A2 Antigen; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D051034:Inhibitor of Apoptosis Proteins; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009369:Neoplasms / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D000077022:Survivin; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: C107489:BIRC5 protein, human; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D051034:Inhibitor of Apoptosis Proteins; D053645:Interleukin-2 Receptor alpha Subunit; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D000077022:Survivin