Cyclic QDE peptide increases fertilization rates and provides healthy pups in mouse.
OBJECTIVE
To evaluate the effects of cyclic QDE peptide (cQDE), derived from sperm fertilin beta (ADAM2), in mouse in vitro fertilization (IVF) and its harmlessness on pups after embryo transfer.


METHODS
Prospective in vivo and in vitro study in mice.


METHODS
Murine model in an academic research environment in France.


METHODS
Normal B6CBF1 mice.


METHODS
Indirect immunofluorescence was used to evaluate cQDE binding to oolemma. The IVF assays were run in presence of the species-specific tripeptide (cQDE at 100 microM), and embryos were transferred in pseudopregnant mice. Controls were obtained by natural mating and IVF in regular medium followed by embryo transfer.


METHODS
Evaluation of fertilization, litter size, pup fertility and health.


RESULTS
Biotinylated cQDE peptide binds to oocyte plasma membrane and increases gamete fusion in cumulus-intact IVF assay. Litter size as well as pup development after embryo transfer showed no statistically significant differences compared with controls. Pups born from embryos that were incubated with the peptide were as healthy and normally fertile as control mice over at least three generations.


CONCLUSIONS
Cyclic QDE is harmless and improves mouse IVF pregnancy rates. A randomized prospective clinical trial to evaluate the beneficial effect of cyclic FEE on human IVF is required before its clinical use.
Barraud-Lange
Virginie
V
Laboratoire Interaction Gamétique et Fusion des Membranes, EA2717, Institut Alfred Jost, Université Paris Descartes, Paris, France.
Naud-Barriant
Nathalie
N
Ducot
Béatrice
B
Chambris
Stéphane
S
Bomsel
Morgane
M
Wolf
Jean-Philippe
JP
Ziyyat
Ahmed
A
D051722:ADAM Proteins / Q000737:chemistry; D000818:Animals; D019921:Biotinylation; D002462:Cell Membrane / Q000502:physiology; D005260:Female; D000072196:Fertilins; D005298:Fertility / Q000187:drug effects / Q000502:physiology; D005306:Fertilization / Q000187:drug effects / Q000502:physiology*; D005307:Fertilization in Vitro / Q000662:veterinary; D008098:Litter Size / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008562:Membrane Glycoproteins / Q000737:chemistry; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010456:Peptides, Cyclic / Q000494:pharmacology*; D011247:Pregnancy; D013094:Spermatozoa
D008562:Membrane Glycoproteins; D010456:Peptides, Cyclic; D051722:ADAM Proteins; C000606454:ADAM2 protein, human; C000606458:Adam2 protein, mouse; D000072196:Fertilins