Mechano-perception in Chara cells: the influence of salinity and calcium on touch-activated receptor potentials, action potentials and ion transport.
This paper investigates the impact of increased salinity on touch-induced receptor and action potentials of Chara internodal cells. We resolved underlying changes in ion transport by current/voltage analysis. In a saline medium with a low Ca(2+) ion concentration [(Ca(2+))(ext)], the cell background conductance significantly increased and proton pump currents declined to negligible levels, depolarizing the membrane potential difference (PD) to the excitation threshold [action potential (AP)(threshold)]. The onset of spontaneous repetitive action potentials further depolarized the PD, activating K(+) outward rectifying (KOR) channels. K(+) efflux was then sustained and irrevocable, and cells were desensitized to touch. However, when [Ca(2+)](ext) was high, the background conductance increased to a lesser extent and proton pump currents were stimulated, establishing a PD narrowly negative to AP(threshold). Cells did not spontaneously fire, but became hypersensitive to touch. Even slight touch stimulus induced an action potential and further repetitive firing. The duration of each excitation was extended when [Ca(2+)](ext) was low. Cell viability was prolonged in the absence of touch stimulus. Chara cells eventually depolarize and die in the saline media, but touch-stimulated and spontaneous excitation accelerates the process in a Ca(2+)-dependent manner. Our results have broad implications for understanding the interactions between mechano-perception and salinity stress in plants.
Shepherd
Virginia A
VA
Department of Biophysics, School of Physics, The University of New South Wales, Sydney, New South Wales 2052, Australia. vas@phys.unsw.edu.au
Beilby
Mary J
MJ
Al Khazaaly
Sabah A S
SA
Shimmen
Teruo
T
D000200:Action Potentials; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival; D044784:Chara / Q000378:metabolism*; D017136:Ion Transport; D040542:Mechanotransduction, Cellular; D008954:Models, Biological; D018408:Patch-Clamp Techniques; D010812:Physical Stimulation; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism; D017494:Proton Pumps / Q000378:metabolism
D015221:Potassium Channels; D017494:Proton Pumps; D011188:Potassium; D002118:Calcium