Determination and pharmacokinetics of ascaridole in rat plasma by gas chromatography-mass spectrometry.
A rapid and sensitive method for determination of ascaridole in rat plasma was developed based on gas chromatography-mass spectrometry (GC/MS). The analyte and internal standard (IS), naphthalene, were extracted from plasma with ethyl acetate and then separated by GC on a HP-5MS capillary analytical column (30 m x 0.25 mm, 0.25 microm) and determined by a quadrupole mass spectrometer detector operated under selected ion monitoring mode (SIM). Excellent linearity was found to be from 10 to 1,000 ng/mL with a lower limit of quantitation (LLOQ) of 10 ng/mL. The accuracy was between 85.3% and 114.0%, and the precision was less than 14.5% (intra- and inter-day). The method was successfully applied to investigate the pharmacokinetic study of ascaridole in rats after a single oral dose of 30, 60 and 120 mg/kg, respectively.
Chu
Yang
Y
Tasly R&D Institute, Tianjin Tasly Group Co. Ltd., Tianjin 300410, China.
Li
Wei
W
Han
Jianping
J
Mo
Hongmei
H
Li
Xinxin
X
Zhou
Shuiping
S
Zhu
Yonghong
Y
Liu
Changxiao
C
D000284:Administration, Oral; D000818:Animals; D000871:Anthelmintics / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D019540:Area Under Curve; D000081005:Cyclohexane Monoterpenes; D004355:Drug Stability; D005215:Fasting; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D006207:Half-Life; D008297:Male; D015394:Molecular Structure; D039821:Monoterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D010545:Peroxides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D000871:Anthelmintics; D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D010545:Peroxides; C043372:ascaridole