Modulating human connective tissue progenitor cell behavior on cellulose acetate scaffolds by surface microtextures.
Soft lithography techniques are used to fabricate cellulose acetate (CA) scaffolds with surface microtextures to observe growth characteristics of the progeny of human marrow-derived connective tissue progenitor cells (CTPs). Human CTPs were collected and cultured on CA scaffolds comprised postmicrotextures and smooth surfaces for up to 30 days. Cells on the smooth surfaces migrated without any preferred orientation for up to 30 days. On microtextures, cells tended to direct their processes toward posts and other cells on day 9. By day 30, cells on microtextures covered the surface with extracellular matrix. DNA quantification revealed approximately threefold more cells on microtextures than on the smooth surfaces. The alkaline phosphatase (AP) mRNA expression was slightly higher on smooth surfaces on day 9. However, by day 30, AP mRNA showed higher expression on microtextures. The mRNA expression of collagen type I was increased on microtextures by day 30, whereas smooth surfaces demonstrated similar expression. The osteocalcin mRNA expression was increased on postmicrotextures relative to smooth surfaces by day 30.
Kim
Eun Jung
EJ
BioMEMS Laboratory, Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA.
Boehm
Cynthia A
CA
Fleischman
Aaron J
AJ
Muschler
George F
GF
Kostov
Yordan V
YV
Roy
Shuvo
S
D001854:Bone Marrow Cells; D018929:Cell Culture Techniques; D002465:Cell Movement; D002482:Cellulose / Q000031:analogs & derivatives* / Q000494:pharmacology; D003094:Collagen / Q000235:genetics; D003238:Connective Tissue; D004247:DNA / Q000032:analysis; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D015675:Osteocalcin / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013234:Stem Cells / Q000166:cytology*; D013499:Surface Properties; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*
D012333:RNA, Messenger; D015675:Osteocalcin; C005062:acetylcellulose; D002482:Cellulose; D003094:Collagen; D004247:DNA