title: Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma.
abstract: MicroRNAs are a group of small non-coding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature microRNAs in human cancers. We characterized the alteration in expression of a select group of microRNAs in primary peritoneal carcinoma relative to matched cases of ovarian serous carcinoma. MicroRNA expression was analysed using semi-quantitative stem-loop RT-PCR on a set of 34 formalin-fixed paraffin-embedded samples. Protein expression of p53 and bcl-2 was quantified in the corresponding tissue microarray. We provide definitive evidence that there is downregulation of a select group of microRNAs in tumours meeting Gynaecological Oncology Group criteria for primary peritoneal carcinoma relative to ovarian serous carcinoma. Specifically, we show decreased p53 expression and downregulation of miR-195 and miR-497 from the microRNA cluster site at chromosome 17p13.1 in primary peritoneal carcinoma relative to ovarian serous carcinoma. miR-195 and miR-497 may have potential roles as tumour-suppressor genes in primary peritoneal tumourigenesis.
authors.affiliation: Department of Histopathology, Trinity College Dublin, Dublin, Ireland. flavinr@tcd.ie
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Flavin
authors.forename: Paul C
authors.initials: PC
authors.lastname: Smyth
authors.forename: Alexandros
authors.initials: A
authors.lastname: Laios
authors.forename: Sharon A
authors.initials: SA
authors.lastname: O'Toole
authors.forename: Ciara
authors.initials: C
authors.lastname: Barrett
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Finn
authors.forename: Susan
authors.initials: S
authors.lastname: Russell
authors.forename: Martina
authors.initials: M
authors.lastname: Ring
authors.forename: Karen M
authors.initials: KM
authors.lastname: Denning
authors.forename: Jinghuan
authors.initials: J
authors.lastname: Li
authors.forename: Sinead T
authors.initials: ST
authors.lastname: Aherne
authors.forename: Dania A
authors.initials: DA
authors.lastname: Sammarae
authors.forename: Natasha A
authors.initials: NA
authors.lastname: Aziz
authors.forename: Araibi
authors.initials: A
authors.lastname: Alhadi
authors.forename: Brian L
authors.initials: BL
authors.lastname: Sheppard
authors.forename: Kai
authors.initials: K
authors.lastname: Lao
authors.forename: Orla M
authors.initials: OM
authors.lastname: Sheils
authors.forename: John J
authors.initials: JJ
authors.lastname: O'Leary
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D002886:Chromosomes, Human, Pair 17; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D010534:Peritoneal Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: C505362:MIRN16 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53