Expression and subcellular localization of ORC1 in Leishmania major.
The mechanism of DNA replication is highly conserved in eukaryotes, with the process being preceded by the ordered assembly of pre-replication complexes (pre-RCs). Pre-RC formation is triggered by the association of the origin replication complex (ORC) with chromatin. Leishmania major appears to have only one ORC ortholog, ORC1. ORC1 in other eukaryotes is the largest of the ORC subunits and is believed to play a significant role in modulating replication initiation. Here we report for the first time, the cloning of ORC1 from L. major, and the analysis of its expression in L. major promastigotes. In human cells ORC1 levels have been found to be upregulated in G1 and subsequently degraded, thus playing a role in controlling replication initiation. We examine the subcellular localization of L. major ORC1 in relation to the different stages of the cell cycle. Our results show that, unlike what is widely believed to be the case with ORC1 in human cells, ORC1 in L. major is nuclear at all stages of the cell cycle.
Kumar
Diwakar
D
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
Mukherji
Agnideep
A
Saha
Swati
S
D000818:Animals; D002455:Cell Division; D003001:Cloning, Molecular; D004261:DNA Replication; D006801:Humans; D018320:Leishmania major / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D051738:Origin Recognition Complex / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
D051738:Origin Recognition Complex; D015800:Protozoan Proteins