[Protective effects of estrogen on mitochondria in human umbilical vascular endothelial cells].
OBJECTIVE
To investigate the protective effects of estrogen on the mitochondria in human umbilical vascular endothelial cells (HUVECs).


METHODS
HUVECs were exposed to H2O2 at 250 micromol/L for 4 h with or without pretreatment with 17-estradiol (E2) and ICI182780. Complex IV activity of the cells was measured with chromometry, and 2, 7-dichlorofluorescein diacetate (DCFH-DA) was used to determine intracellular reactive oxygen species (ROS). Intracellular adenosine triphosphate (ATP) level was quantified with a luciferin- and luciferase-based assay.


RESULTS
Compared to the blank control group, H2O2 caused a decrease in complex IV activity, intracellular ATP level, and the cell viability, but elevated intracellular ROS. E2 pretreatment of cells significantly attenuated these effects of H2O2 exposure. ICI182780 administered prior to E2 pretreatment antagonized the protective effects of E2 against H2O2 exposure.


CONCLUSIONS
E2 offers mitochondrial protective effects on HUVECs, which is mediated by the estrogen receptors.
Chen
Guo-dong
GD
Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. dgchenguodong@sina.com
Wu
Sai-zhu
SZ
Ruan
Yuan-jun
YJ
Peng
Hui-ru
HR
Xing
Xiao-wen
XW
Yin
Meng-zhuo
MZ
Jian
Zheng-wei
ZW
Wang
Yu-yan
YY
D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology*
D004967:Estrogens; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D003576:Electron Transport Complex IV