Reverse transcription loop-mediated isothermal amplification for the detection of highly pathogenic porcine reproductive and respiratory syndrome virus.
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the open reading frames 1a of highly pathogenic porcine reproductive and respiratory syndrome virus genome was developed. The 10 reference strains, 1 clinical isolation strain and 122 positive samples were tested. Positive reactions were confirmed for all strains and specimens by reverse transcription loop-mediated isothermal amplification and nested reverse transcription polymerase chain reaction (RT-PCR). The results showed this detection technique is more reliable and convenient for rapid and sensitive diagnosis of highly pathogenic porcine reproductive and respiratory syndrome virus infection.
Chen
Hao-tai
HT
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Zhang
Jie
J
Sun
De-hui
DH
Ma
Li-na
LN
Liu
Xiang-tao
XT
Quan
Kai
K
Liu
Yong-sheng
YS
D000818:Animals; D001769:Blood / Q000821:virology; D008168:Lung / Q000821:virology; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012661:Semen / Q000821:virology; D012680:Sensitivity and Specificity; D013552:Swine; D013997:Time Factors