title: Cloning, expression and purification of cytochrome c(6) from the brown alga Hizikia fusiformis and complete X-ray diffraction analysis of the structure.
abstract: The primary sequence of cytochrome c(6) from the brown alga Hizikia fusiformis has been determined by cDNA cloning and the crystal structure has been solved at 1.6 A resolution. The crystal belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 84.58, c = 232.91 A and six molecules per asymmetric unit. The genome code, amino-acid sequence and crystal structure of H. fusiformis cytochrome c(6) were most similar to those of red algal cytochrome c(6). These results support the hypothesis that brown algae acquired their chloroplasts via secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host.
authors.affiliation: Bio-organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa-shi, Kanagawa 252-8510, Japan.
authors.forename: Hideharu
authors.initials: H
authors.lastname: Akazaki
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Kawai
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Chida
authors.forename: Yuichirou
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Mao
authors.initials: M
authors.lastname: Hirayama
authors.forename: Ken
authors.initials: K
authors.lastname: Hoshikawa
authors.forename: Satoru
authors.initials: S
authors.lastname: Unzai
authors.forename: Wataru
authors.initials: W
authors.lastname: Hakamata
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nishio
authors.forename: Sam Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Tadatake
authors.initials: T
authors.lastname: Oku
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D045365:Cytochromes c6 / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers; D000459:Phaeophyceae / Q000201:enzymology*; D011487:Protein Conformation
chemical_list: D045365:Cytochromes c6; D017931:DNA Primers