Reduction in rat oocyte fertilizability mediated by S-(1, 2-dichlorovinyl)-L-cysteine: a trichloroethylene metabolite produced by the glutathione conjugation pathway.
Trichloroethylene (TCE), a commonly used industrial degreasing solvent and environmental toxicant, reduces rat oocyte fertilizability by an incompletely understood mechanism. Previous evidence implicated cytochrome P450 dependent oxidation of TCE. The current study investigated a second pathway, glutathione conjugation using S-(1,2-dichlorovinyl)-L-cysteine (DCVC), a mutagenic and cytotoxic TCE-metabolite. In vitro exposure of oocytes and in vivo exposure of females to DCVC significantly reduced oocyte fertilizability (63% vs. 26%; p < 0.005 and 60% vs. 36%; p < 0.005, respectively). Reduced fertilizability of oocytes following in vivo TCE exposure may be mediated partially by the glutathione conjugation pathway.
Wu
Katherine Lily
KL
Department of Animal Science, University of California, Davis, CA 95616, USA.
Berger
Trish
T
D000818:Animals; D001711:Biotransformation; D001835:Body Weight / Q000187:drug effects; D003545:Cysteine / Q000031:analogs & derivatives* / Q000633:toxicity; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects; D012997:Solvents; D014241:Trichloroethylene / Q000493:pharmacokinetics*
D012997:Solvents; D014241:Trichloroethylene; C039961:S-(1,2-dichlorovinyl)cysteine; D005978:Glutathione; D003545:Cysteine