title: Fibromodulin-deficient mice reveal dual functions for fibromodulin in regulating dental tissue and alveolar bone formation.
abstract: The extracellular matrix of newborn, 7- and 21-day-old fibromodulin-deficient (Fmod KO) mice was compared with age-matched wild-type (WT) mice. Western blotting of proteins from 21-day-old WT mice revealed that the molecular weight of Fmod is smaller in dental tissues (approx. 40 kDa) compared to alveolar bone extracts (approx. 52 kDa). Dentin matrix protein1 (DMP1) was slightly increased in Fmod KO versus WT tooth extracts. After chondroitinase ABC digestion, dentin sialophosphoprotein (DSPP) appeared as 2 strong bands (approx. 150 and 70 kDa) in incisors from 21-day-old Fmod KO mice, whereas the smaller-sized species of DSPP was nearly absent in WT molars and no difference was detected between WT and KO mice in molars. Dentin mineralization was altered in newborn and 7-day-old KO mice, but seemed normal in 21-day-old KO mice. DMP1 and DSPP may be involved in compensatory mechanisms. The enamel had a twisted appearance and looked porous at day 21 in KO incisor, and the outer aprismatic layer was missing in the molar. Alveolar bone formation was enhanced in Fmod KO mice at days 0 and 7, whereas no difference was detected at day 21. We conclude that Fmod may control dental tissue formation and early maturation, where it acts mostly as an inhibitor in alveolar bone accumulation, excerpting its effects only at early developing stages. These dual functions may be related to the different forms of Fmod found in bone versus teeth.
authors.affiliation: Laboratoire Réparation et Remodelage des Tissues Orofaciaux EA 2496, Faculté de Chirurgie Dentaire, Université Paris Descartes, Montrouge, France. mgoldod@aol.com
authors.forename: Michel
authors.initials: M
authors.lastname: Goldberg
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Ono
authors.forename: Dominique
authors.initials: D
authors.lastname: Septier
authors.forename: Mireille
authors.initials: M
authors.lastname: Bonnefoix
authors.forename: Tina M
authors.initials: TM
authors.lastname: Kilts
authors.forename: Yanming
authors.initials: Y
authors.lastname: Bi
authors.forename: Mildred
authors.initials: M
authors.lastname: Embree
authors.forename: Laurent
authors.initials: L
authors.lastname: Ameye
authors.forename: Marian F
authors.initials: MF
authors.lastname: Young
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003743:Dental Enamel / Q000166:cytology / Q000378:metabolism; D003804:Dentin / Q000166:cytology / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000172:deficiency* / Q000378:metabolism; D000071235:Fibromodulin; D051379:Mice; D010012:Osteogenesis / Q000502:physiology*; D011509:Proteoglycans / Q000172:deficiency* / Q000378:metabolism; D014070:Tooth / Q000166:cytology / Q000378:metabolism*
chemical_list: D016326:Extracellular Matrix Proteins; C000604332:Fmod protein, mouse; D011509:Proteoglycans; D000071235:Fibromodulin