[Effect of regulating intracellular ROS with antioxidants on the ex vivo expansion of cord blood CD34+ cells].
OBJECTIVE
To investigate the effect of regulating intracellular ROS with antioxidants on the ex vivo expansion of cord blood CD34(+) cells.


METHODS
The levels of reactive oxygen species (ROS) in cord blood CD34(+) cells were reduced by superoxide dismutase (SOD), catalase (CAT) or N-acetylcysteine (NAC) in ex vivo expansion. The expansion of CD34(+) cells reducing ROS levels with antioxidant was studied.


RESULTS
It was observed that the generation of ROS was increased markedly by the cytokine combination. ROS was eliminated by antioxidant effectively. With the addition of antioxidant at different concentration, ROS was reduced to different levels. The percentage of CD34(+) cells and CD34(+)CD38(-) cells, the colony growth of colony-forming cells (CFC) and the re-expansion capability of CD34(+) cells were enhanced by 2 000 U/mL SOD, 200 U/mL CAT or 2 mmol/L NAC. However, its effect on fold expansion of CD34(+) cells was not significant. The expansion of the cells was inhibited by 8 000 U/mL SOD, 1 000 U/mL CAT or 5 mmol/L NAC.


CONCLUSIONS
The proportion of hematopoietic stem and progenitor cells in the culture substance was increased markedly with the addition of low dose antioxidants in ex vivo expansion.
Fan
Jin-Li
JL
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. fjinli@hotmail.com
Cai
Hai-Bo
HB
Tan
Wen-Song
WS
D000111:Acetylcysteine / Q000494:pharmacology; D018952:Antigens, CD34 / Q000378:metabolism*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000494:pharmacology
D018952:Antigens, CD34; D000975:Antioxidants; D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase; D000111:Acetylcysteine