[Relationship between single nucleotide polymorphism-56 of calpain-10 gene and glucose and lipid metabolism in polycystic ovary syndrome patients].
OBJECTIVE
To investigate the relationship between single nucleotide polymorphism-56 (SNP-56) in calpain-10 (CAPN-10) gene and polycystic ovary syndrome (PCOS) in Chinese.


METHODS
The genotypes of SNP-56 of CAPN-10 were determined through polymerase chain reaction Tm-shift genotyping method in 638 local women in Shandong Province. Among them, 334 were patients with PCOS (PCOS group) and 304 were normal women (control group). The baseline parameters including levels of serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), estradiol (E2), testosterone (T) and lipid,as well as the body mass index (BMI) and waist/hip ratio (WHR) were measured. Glucose tolerance and insulin releasing before and after loading with 75 g of glucose were also assayed.


RESULTS
(1) The frequencies of two allelotypes or three genotypes did not differ between PCOS women and normal women (P > 0.05). (2) In PCOS group, patients with AA genotype had a significantly higher plasma glucose of 180 minutes OGTT (5.7 +/- 2. 2)mmol/L [P < 0.01 compared to GA genotype (4.9 +/- 1.2) mmol/L, P < 0.01 compared to GG genotype (4.9 +/- 1.4) mmol/L] and serum total cholesterol (TC) level (4.9 +/- 1.0) mmol/L [P < 0.05 compared to GA genotype (4.5 +/- 0.9) mmol/L]. (3) Compared to PCOS patients with GA + GG genotype (P < 0.05, P < 0.01) or GG genotype (P < 0.05, P < 0.01), there was significantly higher attack rate of diabetes and tumor in the family history of patients with AA genotype.


CONCLUSIONS
These findings suggest that CAPN-10 gene SNP-56 which may not contribute to the genetic susceptibility of PCOS plays a role in glucose and lipid metabolism in Chinese PCOS patients. It may also be correlated with attack rate of diabetes and tumor in the family history of PCOS patients.
Diao
Xing-Hua
XH
Reproductive Medical Center, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Shi
Yu-Hua
YH
Gao
Qin
Q
Wang
Lai-Cheng
LC
Tang
Rong
R
Chen
Zi-Jiang
ZJ
D000293:Adolescent; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D002154:Calpain / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008055:Lipids / Q000097:blood*; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D014280:Triglycerides / Q000097:blood; D055815:Young Adult
D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D014280:Triglycerides; D002154:Calpain; C415197:calpain 10