GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper.
A full length cDNA clone encoding Capsicum annuum GDSL-lipase 1 (CaGL1) was isolated by microarray analysis. The expression of CaGL1 was triggered by methyl jasmonic acid (MeJA), an important signal in abiotic/biotic stress response. However, the expression of this gene was not increased by the application of salicylic acid (SA) or ethylene treatment. And, local/systemic wounding stimuli resulted in rapid accumulation of CaGL1 mRNA. However, CaGL1 was not specifically induced during the hypersensitive response upon Tobacco mosaic virus (TMV) inoculation. By using a virus-induced gene silencing (VIGS)-based reverse genetic approach, it was observed that the suppression of CaGL1 attenuates the expression of Capsicum annuumpathogenesis-related protein 4 (CaPR-4) during wound stress. However, the CaPR-4 transcript level induced by TMV was not regulated by CaGL1 expression. These results indicate that CaGL1 may be involved in signaling pathway of MeJA and/or the wound responses through CaPR-4 expression modulation.
Kim
Ki-Jeong
KJ
School of Life Sciences and Biotechnology/Plant Signaling Network Research Center, Korea University, 1, 5-ga, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
Lim
Jee Hyuck
JH
Kim
Min Jung
MJ
Kim
Taesung
T
Chung
Hyen Mi
HM
Paek
Kyung-Hee
KH
D000595:Amino Acid Sequence; D002212:Capsicum / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology; D015536:Down-Regulation; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020156:Salicylic Acid / Q000494:pharmacology; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant; C011006:jasmonic acid; C036216:ethylene; D002265:Carboxylic Ester Hydrolases; D020156:Salicylic Acid