title: Cations residing at the selectivity filter of the voltage-gated Ca2+-channel modify fusion-pore kinetics.
abstract: Strontium (Sr(2+)), Barium (Ba(2+)) and Lanthanum (La(3+)) can substitute for Ca(2+) in driving synaptic transmission during membrane depolarization. Ion recognition at the polyglutamate motif (EEEE), comprising the channel selectivity-filter, during voltage-driven transitions, controls the kinetics of the voltage-gated calcium channel (VGCC) and its interactions with the synaptic proteins. We tested the effect of different charge carriers on evoked-release, as a means of exploring the involvement of VGCC in the fusion pore configuration. Employing amperometry recordings in single bovine chromaffin cells we show that the size of the fusion pore, designated by the 'foot'-amplitude, was increased when Ba(2+) substituted for Ca(2+) and decreased, with La(3+). The fusion pore stability, indicated by 'foot'-width, was decreased in La(3+). Also, the mean open time of the fusion pore (tau(fp)) was significantly lower in Sr(2+) and La(3+) compared to Ba(2+) and Ca(2+). These cations when occupying the selectivity filter reduced the spike frequency in the order of Ca(2+) > Sr(2+) > Ba(2+) > La(3+), which is parallel to the reduction in total catecholamine release. The correlation between ion binding at the selectivity filter and fusion pore properties supports a model in which the Ca(2+) channel regulates secretion through a site at the selectivity filter, upstream to cation entry into the cell.
authors.affiliation: Institute of Life Sciences, Department of Biological Science, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Merav
authors.initials: M
authors.lastname: Marom
authors.forename: Ariel
authors.initials: A
authors.lastname: Sebag
authors.forename: Daphne
authors.initials: D
authors.lastname: Atlas
mesh_terms: D000818:Animals; D001464:Barium / Q000737:chemistry; D002118:Calcium / Q000737:chemistry; D015220:Calcium Channels / Q000378:metabolism*; D002395:Catecholamines / Q000737:chemistry; D002412:Cations; D002417:Cattle; D019439:Chromaffin Cells / Q000166:cytology; D007700:Kinetics; D007811:Lanthanum / Q000737:chemistry; D008564:Membrane Potentials; D008956:Models, Chemical; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D002395:Catecholamines; D002412:Cations; D001464:Barium; D007811:Lanthanum; D002118:Calcium