Generation of neuronal-like cells from umbilical cord blood-derived mesenchymal stem cells of a RFP-transgenic cloned cat.
Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are multipotent adult stem cells, which can differentiation into cells of connective tissue and neural lineages. This study investigated the potential for neuronal differentiation of red fluorescent protein (RFP)-transgenic cat UCB-derived MSCs. The cells were cultured in pre-induction medium for 24 hr and in neuronal-induction medium for 72 hr. Immunofluorescent staining showed that 6.85% of the total cells were beta III-tubulin-positive, 3.37% were neurofilament light (NF-L)-positive and 7.04% were neurofilament medium (NF-M)-positive. A beta III-tubulin band was detected by western blot analysis. Our results demonstrate that RFP-transgenic UCB-derived MSCs can be differentiated into neuronal cells in vitro. Thus, RFP-transgenic MSCs could provide alternative tracing material for stem cell transplantation.
Jin
Guang-Zhen
GZ
Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University, Jinju, GyeongNam Province, South Korea.
Yin
Xi-Jun
XJ
Yu
Xian-Feng
XF
Cho
Su-Jin
SJ
Choi
Eu-Gene
EG
Lee
Young-S
YS
Jeon
Jin-Tae
JT
Yee
Sung-Tae
ST
Kong
Il-Keun
IK
D000818:Animals; D030801:Animals, Genetically Modified; D002415:Cats / Q000097:blood*; D002454:Cell Differentiation / Q000502:physiology; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry / Q000662:veterinary; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence / Q000662:veterinary; D009474:Neurons / Q000166:cytology*; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein