Reliable enzyme-linked immunosorbent assay for the determination of walnut proteins in processed foods.
Among food allergens of tree nuts, walnuts are a frequent cause of adverse food reactions in allergic patients. A novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of walnut soluble proteins in processed foods was developed. The sandwich ELISA is highly specific for walnut soluble proteins. The recovery ranged from 83.4 to 123%, whereas the intra- and interassay coefficients of variation were less than 8.8 and 7.2%, respectively. This study showed that the proposed method is a reliable tool for detection in the presence of hidden walnut proteins in processed foods.
Doi
Hirotoshi
H
Morinaga Institute of Biological Science Inc., 2-1-16 Sachiura, Kanazawa-ku, Yokohama 236-0003, Japan.
Touhata
Yuki
Y
Shibata
Haruki
H
Sakai
Shinobu
S
Urisu
Atsuo
A
Akiyama
Hiroshi
H
Teshima
Reiko
R
D052179:Antigens, Plant / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling; D031324:Juglans / Q000737:chemistry* / Q000276:immunology; D009754:Nuts / Q000737:chemistry* / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D052179:Antigens, Plant; D010940:Plant Proteins