The cell biology of DNA methylation in mammals.
In this review, we will provide a brief reminder of epigenetic phenomena in general, and DNA methylation in particular. We will then underline the characteristics of the in vivo organization of the genome that limit the applicability of in vitro results. We will use several examples to point out the connections between DNA methylation and nuclear architecture. Finally, we will outline some of the hopes and challenges for future research in the field. The study of DNA methylation, its effectors, and its roles, illustrates the complementarity of in vitro approaches and cell biology.
Prokhortchouk
Egor
E
Center BioEngineering, Russian Academy of Sciences, Prospekt 60-let Oktyabrya 7-1, 117312 Moscow, Russia.
Defossez
Pierre-Antoine
PA
D000818:Animals; D003596:Cytosine / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D004261:DNA Replication; D044127:Epigenesis, Genetic; D005786:Gene Expression Regulation; D008322:Mammals / Q000235:genetics*; D051783:Methyl-CpG-Binding Protein 2 / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000378:metabolism; D009690:Nucleic Acid Conformation; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D051783:Methyl-CpG-Binding Protein 2; D009687:Nuclear Proteins; D014157:Transcription Factors; C118822:Zbtb33 protein, mouse; D003596:Cytosine; D004247:DNA; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases