Cytoprotective roles of senescence marker protein 30 against intracellular calcium elevation and oxidative stress.
Senescence marker protein 30 (SMP30) is identified as an important aging marker molecule and known to play multifunctional roles as an intracellular calcium regulatory protein in the signaling process. To elucidate the functional significance of SMP30, we established the stably transfected P19 cell line with SMP30 expression vector. Overexpression of SMP30 slightly suppressed the proliferation of P19 cells. However, SMP30 overexpression was cytoprotective against calcium-mediated stress such as calcium ionophore (A23187), and thapsigargin. We found that SMP30 overexpression reduced the elevated intracellular calcium levels induced by A23187, but not by thapsigargin. In addition, SMP30 transfected P19 cells were more protective to tert-butylhydroperoxide induced cytotoxicity, indicating the antioxidative properties of SMP30. Taken together, our results suggest that external calcium regulation and antioxidant properties are involved in the cytoprotective mechanism of SMP30.
Son
Tae Gen
TG
Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Longevity Life Science and Technology Institutes, Pusan National University, Busan, Korea.
Kim
So Jung
SJ
Kim
Keunho
K
Kim
Min-Sun
MS
Chung
Hae Young
HY
Lee
Jaewon
J
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015153:Blotting, Western; D000001:Calcimycin / Q000037:antagonists & inhibitors / Q000633:toxicity; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000502:physiology*; D002273:Carcinogens / Q000037:antagonists & inhibitors / Q000633:toxicity; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D018384:Oxidative Stress / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019284:Thapsigargin / Q000037:antagonists & inhibitors / Q000633:toxicity; D014162:Transfection
D000975:Antioxidants; D002135:Calcium-Binding Proteins; D002273:Carcinogens; D047908:Intracellular Signaling Peptides and Proteins; C513324:Rgn protein, mouse; D000001:Calcimycin; D019284:Thapsigargin; D002118:Calcium