title: Glucose reactivity with filling materials as a limitation for using the glucose leakage model.
abstract: OBJECTIVE
  To evaluate the reactivity of different endodontic materials and sealers with glucose and to asses the reliability of the glucose leakage model in measuring penetration of glucose through these materials.
  
  
  METHODS
  Ten uniform discs (radius 5 mm, thickness 2 mm) were made of each of the following materials: Portland cement, MTA (grey and white), sealer 26, calcium sulphate, calcium hydroxide [Ca(OH)(2)], AH26,Epiphany, Resilon, gutta-percha and dentine. After storing the discs for 1 week at 37 degrees C and humid conditions, they were immersed in 0.2 mg mL(-1) glucose solution in a test tube. The concentration of glucose was evaluated using an enzymatic reaction after 1 week. Statistical analysis was performed with the anova and Dunnett tests at a significant level of P < 0.05.
  
  
  RESULTS
  Portland cement, MTA, Ca(OH)(2) and sealer 26 reduced the concentration in the test tube of glucose significantly after 1 week (P < 0.05). Calcium sulphate reduced the concentration of glucose, but the difference in concentrations was not significant (P = 0.054).
  
  
  CONCLUSIONS
  Portland cement, MTA, Ca(OH)(2) and sealer 26 react with a 0.2 mg mL(-1) glucose solution. Therefore, these materials should not be evaluated for sealing ability with the glucose leakage model.
authors.affiliation: Department of Cariology Endodontology Pedodontology, Academic Center for Dentistry Amsterdam (ACTA), Amsterdam, the Netherlands. hshemesh@acta.nl
authors.forename: H
authors.initials: H
authors.lastname: Shemesh
authors.forename: E M
authors.initials: EM
authors.lastname: Souza
authors.forename: M-K
authors.initials: MK
authors.lastname: Wu
authors.forename: P R
authors.initials: PR
authors.lastname: Wesselink
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry; D001729:Bismuth / Q000737:chemistry; D017610:Calcium Compounds / Q000737:chemistry; D002126:Calcium Hydroxide / Q000737:chemistry; D002133:Calcium Sulfate / Q000737:chemistry; D003738:Dental Cements / Q000737:chemistry; D003763:Dental Leakage / Q000175:diagnosis*; D003804:Dentin / Q000378:metabolism; D004338:Drug Combinations; D004853:Epoxy Resins / Q000737:chemistry; D005947:Glucose / Q000737:chemistry*; D006171:Gutta-Percha / Q000737:chemistry; D006801:Humans; D006813:Humidity; D007202:Indicators and Reagents; D008422:Materials Testing; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results; D012387:Root Canal Filling Materials / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors; D014025:Titanium / Q000737:chemistry
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; D004853:Epoxy Resins; D007202:Indicators and Reagents; D010087:Oxides; D012387:Root Canal Filling Materials; C080620:Sealer 26; D017640:Silicates; C486893:epiphany sealer; C086631:mineral trioxide aggregate; C486892:resilon sealer; D012834:Silver; C028044:epoxy resin AH-26; D006171:Gutta-Percha; D014025:Titanium; D005947:Glucose; D002126:Calcium Hydroxide; D001729:Bismuth; D002133:Calcium Sulfate