title: NF-kappaB modulates aquaporin-2 transcription in renal collecting duct principal cells.
abstract: Renal tubulo-interstitial inflammation is frequently associated with polyuria and urine concentration defects. This led us to investigate the effects of the major pro-inflammatory nuclear factor kappaB (NF-kappaB) pathway on aquaporin 2 (AQP2) expression by the collecting duct. Using immortalized collecting duct principal cells (mpkCCDcl4), we found that, acting independently of vasopressin, activation of NF-kappaB by lipopolysaccharide (LPS) decreased AQP2 mRNA and protein levels in a time- and dose-dependent manner but did not decrease AQP2 mRNA stability. Consistently, constitutively active IkappaB kinase beta decreased AQP2 expression. The LPS-induced decrease in AQP2 mRNA levels was confirmed in rat kidney slices and was reproduced both under conditions of elevated cAMP concentration and V(2) receptor antagonism. Computer analysis of the AQP2 promoter revealed two putative kappaB elements. Mutation of either kappaB element abolished the LPS-induced decrease of luciferase activity in cells expressing AQP2 promoter-luciferase plasmid constructs. Chromatin immunoprecipitation revealed that LPS challenge decreased p65, increased p50 and p52, and had no effect on RelB and c-Rel binding to kappaB elements of the AQP2 promoter. RNA-mediated interference silencing of p65, p50, and p52 confirmed controlled AQP2 transcription by these NF-kappaB subunits. We additionally found that hypertonicity activated NF-kappaB in mpkCCDcl4 cells, an effect that may counteract the Tonicity-responsive enhancer binding protein (TonEBP)-dependent increase in AQP2 gene transcription. Taken together, these findings indicate that NF-kappaB is an important physiological regulator of AQP2 transcription.
authors.affiliation: Service de Néphrologie, Fondation pour Recherches Médicales and University of Geneva, CH-1211, Geneva 4, Switzerland.
authors.forename: Udo
authors.initials: U
authors.lastname: Hasler
authors.forename: Valérie
authors.initials: V
authors.lastname: Leroy
authors.forename: Un Sil
authors.initials: US
authors.lastname: Jeon
authors.forename: Richard
authors.initials: R
authors.lastname: Bouley
authors.forename: Mitko
authors.initials: M
authors.lastname: Dimitrov
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.forename: Dennis
authors.initials: D
authors.lastname: Brown
authors.forename: H Moo
authors.initials: HM
authors.lastname: Kwon
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Martin
authors.forename: Eric
authors.initials: E
authors.lastname: Féraille
mesh_terms: D000818:Animals; D051399:Aquaporin 2 / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D007249:Inflammation; D007668:Kidney / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology; D008954:Models, Biological; D016328:NF-kappa B / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D051381:Rats; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D051399:Aquaporin 2; D008070:Lipopolysaccharides; D016328:NF-kappa B