title: A PCR-based epidemiological survey of Hepatozoon canis in dogs in Nigeria.
abstract: The prevalence of Hepatozoon canis infections in dogs in Nigeria was surveyed using molecular methods. DNA was extracted from blood samples obtained from 400 dogs. A primer set that amplified the Babesia canis 18S rRNA gene, which has high similarity to the H. canis 18S rRNA gene, was used for the PCR. As a result, samples from 81 dogs (20.3%) produced 757 bp bands, which differed from the 698 bp band that corresponded to B. canis infection. The sequence of the PCR products of 10 samples were determined, all of which corresponded with the H. canis sequence.
authors.affiliation: Department of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Sasaki
authors.forename: Olutayo
authors.initials: O
authors.lastname: Omobowale
authors.forename: Kaisaku
authors.initials: K
authors.lastname: Ohta
authors.forename: Morito
authors.initials: M
authors.lastname: Tozuka
authors.forename: Aya
authors.initials: A
authors.lastname: Matsuu
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Hirata
authors.forename: Helen Oyebukola
authors.initials: HO
authors.lastname: Nottidge
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ikadai
authors.forename: Takashi
authors.initials: T
authors.lastname: Oyamada
mesh_terms: D000818:Animals; D003044:Coccidia / Q000235:genetics / Q000302:isolation & purification*; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology*; D004285:Dogs; D005260:Female; D008297:Male; D009549:Nigeria / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S