title: 2,3,7,8-tetrachlorodibenzo-p-dioxin regulates bovine herpesvirus type 1 induced apoptosis by modulating Bcl-2 family members.
abstract: Exposure to environmental contaminants, like 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), leads to an increased susceptibility to infectious agents. Infection of bovine cells (MDBK) with Bovine Herpesvirus 1 (BHV-1) anticipates virus-induced apoptosis, suggesting an involvement of TCDD in virus infection. Herein we analyzed the effects of TCDD on apoptotic pathway in MDBK cells infected with BHV-1. After 12 h of infection, TCDD induced a significant increase in apoptotic cells. TCDD caused a dose-dependent up-regulation and anticipated activation of caspases 3, 8 and 9, with respect to unexposed groups. TCDD anticipated cleavage of PARP, compared to controls. Furthermore TCDD increased Bax and Bid levels, and decreased Bcl-2 and Bcl-XL levels. Such events took place earlier in exposed than unexposed cells. These results showed that TCDD influences BHV-1 induced apoptosis through members of Bcl-2 family and up-regulating activation of caspases.
authors.affiliation: Department of Pathology and Animal Health, School of Veterinary Medicine, University of Naples Federico II, Naples, Italy. filomena.fiorito@unina.it
authors.forename: Filomena
authors.initials: F
authors.lastname: Fiorito
authors.forename: Gabriella
authors.initials: G
authors.lastname: Marfè
authors.forename: Emma
authors.initials: E
authors.lastname: De Blasio
authors.forename: Giovanna Elvira
authors.initials: GE
authors.lastname: Granato
authors.forename: Marco
authors.initials: M
authors.lastname: Tafani
authors.forename: Luisa
authors.initials: L
authors.lastname: De Martino
authors.forename: Serena
authors.initials: S
authors.lastname: Montagnaro
authors.forename: Salvatore
authors.initials: S
authors.lastname: Florio
authors.forename: Ugo
authors.initials: U
authors.lastname: Pagnini
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002417:Cattle; D002452:Cell Count; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D007242:Herpesvirus 1, Bovine / Q000187:drug effects / Q000502:physiology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*
chemical_list: D000072317:Polychlorinated Dibenzodioxins; D019253:Proto-Oncogene Proteins c-bcl-2; D011065:Poly(ADP-ribose) Polymerases; D020169:Caspases