[Application of T cell responses to novel RD1-encoded mycobacterium tuberculosis gene products for specific detection of human tuberculosis infection].
OBJECTIVE
To evaluate the clinical significance of using T cell responses to three RD1-encoded gene products for diagnosis of mycobacterium tuberculosis (MTB) infection.


METHODS
We used ex vivo gamma-interferon enzyme-linked immunospot assays to test T cell responses to three novel gene products encoded by RD1 (Rv3873, Rv3878, and Rv3879c) in 49 patients (TB group) who were bacteriologically confirmed by positive cultures for MTB and 38 healthy BCG-vaccinated donors (control group). Forty-nine overlapping peptides were tested in all participants.


RESULTS
The T cell response rates to Rv3873, Rv3878, and Rv3879c were 53% (95% CI: 39%-67%), 35% (95% CI: 22%-48%), and 45% (95% CI: 31%-57%), respectively, in the TB group and were 7.9%, 2.6%, and 2.6%, respectively, in the control group.


CONCLUSIONS
Rv3879c peptides can be candidates for inclusion in new T cell-based tests for MTB infection.
Liu
Xiao-Qing
XQ
Department of Infectious Disease, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. liuxq_pumch@yahoo.com.cn
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D001500:BCG Vaccine / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D005260:Female; D006801:Humans; D007118:Immunoassay; D007371:Interferon-gamma / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology*; D055815:Young Adult
D000942:Antigens, Bacterial; D001500:BCG Vaccine; D001426:Bacterial Proteins; D007371:Interferon-gamma