Bioactive 5alpha-pregnane-type steroidal alkaloids from Sarcococca hookeriana.
The bioassay-guided phytochemical investigation of Sarcococca hookeriana with respect to cholinesterase inhibitory properties has yielded two new 5alpha-pregnane-type steroidal alkaloids, hookerianamides J (1) and K (2), along with eight known compounds (3-10). The structures of 1 and 2 were elucidated by spectroscopic methods. These compounds displayed good to moderate activities in vitro against the enzymes acetylcholinesterase (IC 50 8.1-48.5 microM) and butyrylcholinesterase (IC 50 0.4-4.0 microM). Compounds 1-10 were also tested in vitro for their leishmanicidal activity against Leishmania major and for their antibacterial activities against Bacillus subtilis, Micrococcus luteus, Streptococcus faecalis, and Pseudomonas pallida.
Devkota
Krishna P
KP
Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, P.O. Box 100131, 33501 Bielefeld, Germany. devkotakpd@yahoo.com
Lenta
Bruno N
BN
Wansi
Jean D
JD
Choudhary
Muhammad I
MI
Kisangau
Daniel P
DP
Naz
Qamar
Q
Samreen
Sewald
Norbert
N
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D031234:Buxaceae / Q000737:chemistry*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D011278:Pregnanes / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000981:Antiprotozoal Agents; D002800:Cholinesterase Inhibitors; D011278:Pregnanes