Characterization of ApuB, an extracellular type II amylopullulanase from Bifidobacterium breve UCC2003.
The apuB gene of Bifidobacterium breve UCC2003 was shown to encode an extracellular amylopullulanase. ApuB is composed of a distinct N-terminally located alpha-amylase-containing domain which hydrolyzes alpha-1,4-glucosidic linkages in starch and related polysaccharides and a C-terminally located pullulanase-containing domain which hydrolyzes alpha-1,6 linkages in pullulan, allowing the classification of this enzyme as a bifunctional class II pullulanase. A knockout mutation of the apuB gene in B. breve UCC2003 rendered the resulting mutant incapable of growth in medium containing starch, amylopectin, glycogen, or pullulan as the sole carbon and energy source, confirming the crucial physiological role of this gene in starch metabolism.
O'Connell Motherway
Mary
M
Department of Microbiology, Alimentary Pharmabiotic Centre and National University of Ireland, Cork, Western Road, Cork, Ireland.
Fitzgerald
Gerald F
GF
Neirynck
Sabine
S
Ryan
Sinead
S
Steidler
Lothar
L
van Sinderen
Douwe
D
D000687:Amylopectin / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D017353:Gene Deletion; D005936:Glucans / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013213:Starch / Q000378:metabolism
D001426:Bacterial Proteins; D005936:Glucans; C009109:pullulan; D013213:Starch; D006003:Glycogen; D000687:Amylopectin; D006026:Glycoside Hydrolases; C082507:amylopullulanase