title: Zone electrophoresis in an inner-cooling wide-bore electrophoresis system with UV detection.
abstract: A novel, high-performance wide-bore electrophoresis (WE) system with inner-cooling has been developed. By introducing the mode of a shell and tube heat exchanger into this system to remove Joule heat generated during electrophoresis, it is feasible to extend electrophoresis from the conventional capillary (i.d. <100 microm) to a wide-bore tube (i.d. >1000 microm). The wide tube allows the loading of over 1.0 microL of the sample with an LOD of 3.0 x 10(-4) mg/mL (signal-to-noise ratio, 3:1). Satisfactory separations of model compounds have been achieved on the WE system.
authors.affiliation: School of Material Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, P. R. China.
authors.forename: Yugao
authors.initials: Y
authors.lastname: Guo
authors.forename: Danning
authors.initials: D
authors.lastname: Liu
authors.forename: Huaifeng
authors.initials: H
authors.lastname: Wang
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Yuan
authors.forename: James Jianmin
authors.initials: JJ
authors.lastname: Bao
mesh_terms: D019817:Benzoic Acid / Q000032:analysis* / Q000737:chemistry; D003080:Cold Temperature; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006358:Hot Temperature; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D013451:Sulfonic Acids / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D013451:Sulfonic Acids; D014867:Water; D019817:Benzoic Acid