[Variations of lipid peroxidation products and copper, zinc-superoxide dismutase in pathological scars].
OBJECTIVE
To study the variations of lipid peroxidation products and copper, zinc-superoxide dismutase (CuZn-SOD) in pathological scars (hypertrophic scars and keloids).


METHODS
The specimens were gained from patients of voluntary contributions from May 2005 to August 2005. The tissues of hypertrophic scar (10 cases, aged 16-35 years, the mean course of disease was 2.2 years), keloid (10 cases, aged 17-32 years, the mean course of disease was 8 months) and normal skin (8 cases, aged 16-34 years) were obtained. The content of malonaldehyde (MDA)and CuZn-SOD activity were detected by spectrophotometric method. The expression of CuZn-SOD was evaluated by immunohistochemistry technique.


RESULTS
The contents of MDA and CuZn-SOD activity were significantly higher in hypertrophic scars [MDA (1.1390 +/- 0.1067) nmoL/mg prot, CuZn-SOD (31.65 +/- 2.21)U/mg prot, (P < 0.05)] and keloids [MDA (1.1900 +/- 0.074 8) nmoL/mg prot, CuZn-SOD (34.36 +/- 5.01) U/mg prot (P < 0.05)] than those of normal skin tissues [MDA (0.8213 +/- 0.0864) nmoL/mg prot, CuZn-SOD (20.60 +/- 5.56) U/mg prot]. Immunohistochemical studies indicated that the brown particles were CuZn-SOD positive signals, which mainly located cytoplasm in normal skin tissues, hypertrophic scars as well as keloids epidermal keratinocytes and dermal fibroblasts. CuZn-SOD expression evaluation in hypertrophic scars (4.14 +/- 0.90, P < 0.05) and keloids epidermal keratinocytes (4.43 +/- 0.79, P < 0.05) markedly increased when compared with normal skin tissues (2.20 +/- 0.45). The expression of CuZn-SOD in hypertrophic scars (4.00 +/- 0.82, P < 0.05) and keloids dermal fibroblasts (4.43 +/- 0.53, P < 0.05) were significantly higher than that of normal skin tissues (1.60 +/- 0.89). There were no differences in the content of MDA, CuZn-SOD activity and expression evaluation between hypertrophic scars and keloids (P > 0.05).


CONCLUSIONS
In pathological scars, the contents of MDA and CuZn-SOD activity increase and the expressions of CuZn-SOD are enlarged.
Li
Weiren
W
Department of Plastic and Burn Surgery, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, PR China.
Cen
Ying
Y
Wang
Huaisheng
H
Chen
Wei
W
Liu
Daqing
D
D000293:Adolescent; D000328:Adult; D017439:Cicatrix, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D003300:Copper / Q000378:metabolism; D005260:Female; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D012867:Skin / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
D008315:Malondialdehyde; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc