A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis.
A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme. Using Streptomyces griseus chitinase, we have demonstrated that our method is more sensitive and less complicated than the conventional Calcofluor white M2R staining.
Liau
Chun Yi
CY
Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan, R.O.C.; Department of Bioengineering, Tatung University, Taipei 104, Taiwan, ROC.
Lin
Chung-Sheng
CS
D002688:Chitinases / Q000032:analysis* / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004789:Enzyme Activation; D008970:Molecular Weight; D012394:Rosaniline Dyes / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
D012394:Rosaniline Dyes; C024757:Coomassie brilliant blue R; D002688:Chitinases