title: Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye.
abstract: A recombinant laccase from Trametes versicolor in Pichia methanolica was produced constitutively in a defined medium. The recombinant laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration. The molecular weight of the purified laccase was estimated as 64 kDa by SDS-PAGE. The purified recombinant laccase decolorized more than 90% of Remazol Brilliant Blue R (RBBR) initially at 80 mg l(-1) after 16 h at 45 degrees C and pH 5 when 25 U laccase ml(-1) was used. The purified recombinant laccase could efficiently decolorize RBBR without additional redox mediators.
authors.affiliation: Department of Food Science, Tianjin Agriculture College, Tianjin, 300384, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
authors.forename: Fuping
authors.initials: F
authors.lastname: Lu
authors.forename: Minyao
authors.initials: M
authors.lastname: Liu
authors.forename: Tuoping
authors.initials: T
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Pu
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Liang
authors.forename: Chenyun
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000880:Anthraquinones / Q000737:chemistry*; D001487:Basidiomycota / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004396:Coloring Agents / Q000737:chemistry*; D042845:Laccase / Q000302:isolation & purification* / Q000378:metabolism*; D010843:Pichia / Q000235:genetics*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014462:Ultrafiltration
chemical_list: D000880:Anthraquinones; D004396:Coloring Agents; D011994:Recombinant Proteins; D042845:Laccase; C024758:Remazol Brilliant Blue R