Stimulatory constituents of Eclipta prostrata on mouse osteoblast differentiation.
One flavonoid, diosmetin (1), and two isoflavonoids, 3'-hydroxybiochanin A (2) and 3'-O-methylorobol (3), were isolated from the methanol extract of Eclipta prostrata L. by a bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system. All three compounds significantly increased osteoblast differentiation as assessed by the alkaline phosphatase activity..
Lee
Mi Kyeong
MK
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Korea.
Ha
Na Ry
NR
Yang
Hyekyung
H
Sung
Sang Hyun
SH
Kim
Young Choong
YC
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D036601:Eclipta / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D010006:Osteoblasts / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry
C534996:3'-O-methylorobol; C534995:3'-hydroxybiochanin A; D005419:Flavonoids; D007529:Isoflavones; D010936:Plant Extracts; C039602:diosmetin