title: Evaluation of phenylbutazone and poly(amidoamine) dendrimers interactions by a combination of solubility, 2D-NOESY NMR, and isothermal titration calorimetry studies.
abstract: The interactions between phenylbutazone, a well-established nonsteroidal anti-inflammatory drug, with different generations of poly(amidoamine) (PAMAM) dendrimers, have been investigated by aqueous solubility, two dimensional nuclear Overhauser effect spectroscopy (2D-NOESY) and isothermal titration calorimetry (ITC) studies. Solubility results showed that PAMAM dendrimers significantly enhanced the aqueous solubility of phenylbutazone and the solubilization was much influenced by dendrimer concentration, generation, surface function group and pH value. The 2D-NOESY spectra clearly showed that there were several kinds of cross-peaks from NOE interactions between the protons of phenylbutazone and the protons in interior cavities of both generation 6 and generation 3 PAMAM dendrimers. The solubility, 2D-NOESY results and ITC analysis suggest that encapsulation and electrostatic interaction together caused the solubility enhancement of phenylbutazone. The new techniques such as 2D-NOESY and ITC used in this study are useful tools in investigating the interactions between dendrimers and guest molecules.
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Yang
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Li
authors.forename: Yiyun
authors.initials: Y
authors.lastname: Cheng
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Wu
authors.forename: Longping
authors.initials: L
authors.lastname: Wen
authors.forename: Tongwen
authors.initials: T
authors.lastname: Xu
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry*; D002151:Calorimetry; D050091:Dendrimers / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010653:Phenylbutazone / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D012995:Solubility; D017186:Titrimetry; D014867:Water / Q000737:chemistry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D050091:Dendrimers; D004337:Drug Carriers; C531249:Poly(amidoamine); D011073:Polyamines; D014867:Water; D010653:Phenylbutazone