title: First isolation of a novel thermostable antifungal peptide secreted by Aspergillus clavatus.
abstract: A novel antifungal peptide produced by an indigenous fungal strain (VR) of Aspergillus clavatus was purified. The antifungal peptide was enriched in the supernatant after heat treatment at 70 degrees C. The thermostable character was exploited in the first purification step, as purified peptide was obtained after ultrafiltration and reverse phase-HPLC on C18 column application. The purified peptide named "AcAFP" for A. clavatus antifungal peptide, has molecular mass of 5773Da determined by MALDI-ToF spectrometry. The N-terminal sequence showed a notable identity to the limited family of antifungal peptides produced by ascomycetes fungi. The AcAFP activity remains intact even after heat treatment at 100 degrees C for 1h confirming its thermostability. It exhibits a strong inhibitory activity against mycelial growth of several serious human and plant pathogenic fungi: Fusariuym oxysporum, Fusarium solani, Aspergillus niger, Botrytis cinerea, Alternaria solani, whereas AcAFP did not affect yeast and bacterial growth.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, BP "K" 3038-Sfax, Tunisia.
authors.forename: Houda
authors.initials: H
authors.lastname: Skouri-Gargouri
authors.forename: Ali
authors.initials: A
authors.lastname: Gargouri
mesh_terms: D000528:Alternaria / Q000187:drug effects; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000378:metabolism; D001230:Aspergillus / Q000378:metabolism*; D001234:Aspergillus niger / Q000187:drug effects; D020171:Botrytis / Q000187:drug effects; D004355:Drug Stability; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D005670:Fusarium / Q000187:drug effects; D006358:Hot Temperature; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C539765:AFP peptide, Aspergillus clavatus; D000935:Antifungal Agents; D005656:Fungal Proteins