title: Platelet-induced inhibition of tumor cell growth.
abstract: BACKGROUND
  Previous studies have suggested that platelets play a role in hematogenous metastasis of cancer cells by enhancing their survival in and extravasation from the bloodstream. We initiated studies to determine the effect of platelets on the proliferation of tumor cells.
  
  
  METHODS
  Intact platelets or platelet subfractions were prepared and used for co-culture with various tumor cell lines of different major histocompatibility complex (MHC) backgrounds. Proliferation of tumor cells was monitored using a colorigenic method; flow cytometry was used to measure apoptosis or the cell cycle in L1210 cells.
  
  
  RESULTS
  Co-culture of platelets with tumor cells inhibited proliferation of tumor cells in an MHC-independent manner; soluble factors released from platelets as well as physical contact between platelets and tumor cells were involved. Cell-cycle analysis showed that platelets inhibited proliferation mainly through arrest of the cell cycle and inhibition of DNA synthesis. Neither cytotoxicity nor apoptosis mechanisms dominate in the platelet-induced inhibition of tumor cell proliferation.
  
  
  CONCLUSIONS
  We observed that murine platelets inhibit growth of tumor cells in vitro in an MHC-I-independent way, and this inhibition is not limited to specific tumor types, nor is it dependent on cytotoxicity or apoptotic pathways; rather it relies on impairment of the cell cycle.
authors.affiliation: Shandong Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China. Yiqiangwang99@hotmail.com
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001686:Biological Phenomena; D001792:Blood Platelets / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D018920:Coculture Techniques; D004261:DNA Replication / Q000187:drug effects; D008223:Lymphoma / Q000378:metabolism / Q000473:pathology; D008297:Male; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology