title: Differential expression of oxidative stress and inflammation related genes in peripheral blood mononuclear cells in response to a low-calorie diet: a nutrigenomics study.
abstract: Nutrigenomics is a new application of omics technologies in nutritional science. Nutrigenomics aims to identify molecular markers of diet-related diseases and mechanisms of interindividual variability in response to food. The aim of this study was to evaluate peripheral blood mononuclear cells (PBMC) as a model system and readily available source of RNA to discern gene expression signatures in relation to personalized therapy of obesity. PBMC were collected from obese men before and after an 8-week low-calorie diet (LCD) to lose weight. Changes in gene expression before and after the LCD were initially screened using a DNA-microarray platform and validated by qRT-PCR. Global gene expression analysis identified 385 differentially expressed transcripts after the LCD. Further analyses showed a decrease in some specific oxidative stress and inflammation genes. Interestingly, expression of these genes was directly related to body weight, while a lower IL8 gene expression was associated with higher fat mass decrease. Collectively, these observations suggest that PBMCs are a suitable RNA source and model system to perform nutrigenomics studies related to obesity and development of personalized dietary treatments. IL8 gene expression warrant further research as a putative novel biomarker of changes in body fat percentage in response to an LCD.
authors.affiliation: Department of Nutrition and Food Sciences, Physiology and Toxicology, University of Navarra, Pamplona, Spain.
authors.forename: Ana B
authors.initials: AB
authors.lastname: Crujeiras
authors.forename: Dolores
authors.initials: D
authors.lastname: Parra
authors.forename: Fermín I
authors.initials: FI
authors.lastname: Milagro
authors.forename: Estibaliz
authors.initials: E
authors.lastname: Goyenechea
authors.forename: Eider
authors.initials: E
authors.lastname: Larrarte
authors.forename: Javier
authors.initials: J
authors.lastname: Margareto
authors.forename: J Alfredo
authors.initials: JA
authors.lastname: Martínez
mesh_terms: D000328:Adult; D031204:Caloric Restriction; D020869:Gene Expression Profiling; D005819:Genetic Markers; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D054647:Nutrigenomics; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D015431:Weight Loss / Q000235:genetics
chemical_list: C508606:CXCL8 protein, human; D005819:Genetic Markers; D018836:Inflammation Mediators; D016209:Interleukin-8