Preparation of CLA ascorbyl ester with improved volumetric productivity by an ionic liquid-based reaction system.
A new approach to the enzymatic production of conjugated linoleic acid (CLA) ascorbyl ester with a remarkably high volumetric productivity (120-200 g L(-1)) has been developed, in which strong solvation by tOMA.TFA (methyltrioctylammonium trifluoroacetate) enables a high concentration of ascorbic acid to be applied, and in which t-butanol enhances conversion by changing the equilibrium constant of the activity coefficients. This work has experimentally demonstrated the practicability of achieving efficient reactions of polar compounds at high concentrations in ionic liquids without significant loss of enzyme activity.
Chen
Bilian
B
National Food Institute, Technical University of Denmark, Building 227, DK-2800 KgsLyngby, Denmark.
Guo
Zheng
Z
Let
Mette Bruni
MB
Lue
Bena-Marie
BM
Xu
Xuebing
X
D001205:Ascorbic Acid / Q000737:chemistry*; D019149:Bioreactors; D002175:Candida / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D004951:Esterification; D004952:Esters / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids; D044243:Linoleic Acids, Conjugated / Q000737:chemistry / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics; D014269:Trifluoroacetic Acid / Q000737:chemistry; D020002:tert-Butyl Alcohol / Q000737:chemistry
C525963:1-butyl-3-methylimidazolium; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D007093:Imidazoles; D052578:Ionic Liquids; D044243:Linoleic Acids, Conjugated; D000644:Quaternary Ammonium Compounds; C037759:trioctylmethylammonium; D014269:Trifluoroacetic Acid; C093667:Novozyme 435; D008049:Lipase; D020002:tert-Butyl Alcohol; D001205:Ascorbic Acid