title: Quantification of urinary F2-isoprostanes with 4(RS)-F4t-neuroprostane as an internal standard using gas chromatography-mass spectrometry Application to polytraumatized patients.
abstract: Isoprostanes are a family of prostaglandin isomers produced from oxidation of polyunsaturated fatty acids through a non-enzymatic free radical-catalyzed mechanism. Quantification of F(2)-isoprostanes (F(2)-IsoPs) provides a good index of oxidative stress and allows non-invasive assessment of lipid peroxidation in vivo. Since "interferences peaks" at m/z 573 co-elute with d(4)-15-F(2t)-IsoP preferentially used, we propose a new GC-NICI-MS approach to quantify urinary F(2)-IsoPs by using 4(RS)-F(4t)-neuroprostane as the internal standard. This method was applied to quantify urinary F(2)-IsoPs excretion in healthy volunteers and polytraumatized patients. Our results showed a significant increase (p<0.0001) in urinary F(2)-IsoPs in polytraumatized patients compared with healthy volunteers (4.73+/-2.75 ng/mg vs. 0.811+/-0.359 ng/mg creatinine).
authors.affiliation: Department of Biochemistry, Lapeyronie Hospital, Montpellier, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Mas
authors.forename: Françoise
authors.initials: F
authors.lastname: Michel
authors.forename: Alexandre
authors.initials: A
authors.lastname: Guy
authors.forename: Valérie
authors.initials: V
authors.lastname: Bultel
authors.forename: Yoan
authors.initials: Y
authors.lastname: Falquet
authors.forename: Patrick
authors.initials: P
authors.lastname: Chardon
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Rossi
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Cristol
authors.forename: Thierry
authors.initials: T
authors.lastname: Durand
mesh_terms: D016022:Case-Control Studies; D028441:F2-Isoprostanes / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015227:Lipid Peroxidation; D009104:Multiple Trauma / Q000652:urine*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D028441:F2-Isoprostanes