[The cell apoptosis induced by duck reovirus in duck embryo fibroblasts].
Cell apoptosis induced by duck reovirus (DRV)in duck embryo fibroblasts (DEF) was ascertained by light microscope and electron microscopy, DNA Ladder, flow cytometry and fluorescent microscopy. Typical morphological apoptotic features including cell shrinkage and condensation, margination of nuclear chromatin were observed under light microscope and the formation of apoptotic bodies by electron microscopy. DNA ladder was shown by DNA fragment analysis at 24-144h post infection. Flow cytometry showed that the cell apoptosis appeared at 24h and reached it's crest-time at 72-96h, decreased at 144h. Fluorescent microscopy showed that the apoptotic cells which showed green fluorescence appeared at 24h, the number of dead cells which showed red fluorescence increased with the time went by. The results above confirmed that the apoptosis of DEF was successfully induced by DRV.
Zhang
Na
N
Research Center of Avian Disease, College of Animal Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, Sichuan, China. nana821024@yahoo.com.cn
Cheng
An-Chun
AC
Wang
Ming-Shu
MS
Li
Chuan-Feng
CF
Chen
Xiao-Yue
XY
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D053938:DNA Fragmentation; D004372:Ducks; D004625:Embryo, Nonmammalian / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure / Q000821:virology*; D005434:Flow Cytometry; D054884:Host-Pathogen Interactions; D046529:Microscopy, Electron, Transmission; D012087:Reoviridae / Q000502:physiology*