Molecular and cytogenetic characterization of repetitive DNA in the Antarctic polyplacophoran Nuttallochiton mirandus.
Two highly repeated DNAs, designated NmE1/NmE2 and NmE5, were identified by EcoRV digestion in the chiton Nuttallochiton mirandus (Mollusca: Polyplacophora). The comparison of the sequences obtained showed high similarity in 5' and 3' regions and the NmE5 sequence displayed an inserted sequence that might arise from a transposable element. Southern blotting analyses suggested a tandem organization of both satellite DNA families identified. Moreover, dot blot analyses, performed on several molluscan species, revealed a different degree of conservation of the repeated DNAs. Fluorescence in-situ hybridizations (FISH) on metaphase chromosomes showed that both satellite DNAs are located at centromeric regions.
Biscotti
Maria Assunta
MA
Istituto di Biologia e Genetica, Facoltà di Scienze, Università Politecnica delle Marche, via Brecce Bianche, I-60131, Ancona, Italy.
Barucca
Marco
M
Capriglione
Teresa
T
Odierna
Gaetano
G
Olmo
Ettore
E
Canapa
Adriana
A
D000818:Animals; D000864:Antarctic Regions; D001483:Base Sequence; D015139:Blotting, Southern; D002875:Chromosomes / Q000378:metabolism; D020732:Cytogenetic Analysis; D004247:DNA / Q000235:genetics; D016678:Genome / Q000235:genetics; D017403:In Situ Hybridization; D049469:Meiotic Prophase I; D008677:Metaphase; D008969:Molecular Sequence Data; D049916:Polyplacophora / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D016415:Sequence Alignment
D004247:DNA