title: A refinery sludge deposition site: presence of nahH and alkJ genes and crude oil biodegradation ability of bacterial isolates.
abstract: 204 bacterial isolates from four Greek refinery sludge deposition sites were investigated for the presence of nahH and alkJ genes encoding key enzymes of both aromatic and aliphatic hydrocarbon degradation pathways by PCR and DNA hybridisation. Members of Pseudomonas, Acinetobacter, Bacillus, Rhodococcus and Arthrobacter play important role in bioremediation processes in sandy/loam soil contaminated with oil and nahH and alkJ genes were present in the 73% of the isolates. Consortia of bacterial isolates that were used for biodegradation of aliphatic and aromatic hydrocarbons in crude oil using liquid cultures exhibited rates from 35% to 48% within 10 days of incubation.
authors.affiliation: Faculty of Biology, Department of Botany, Microbiology Group, National and Kapodistrian University of Athens, 15781 Athens, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Arvanitis
authors.forename: Efstathios A
authors.initials: EA
authors.lastname: Katsifas
authors.forename: Kalliopi I
authors.initials: KI
authors.lastname: Chalkou
authors.forename: Christos
authors.initials: C
authors.lastname: Meintanis
authors.forename: Amalia D
authors.initials: AD
authors.lastname: Karagouni
mesh_terms: D000150:Acinetobacter / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D001407:Bacillus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D001420:Bacteria, Aerobic / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase / Q000235:genetics* / Q000378:metabolism; D004275:DNA, Ribosomal; D004787:Environmental Pollution; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010578:Petroleum; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D012240:Rhodococcus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D004275:DNA, Ribosomal; D006841:Hydrocarbons, Aromatic; D010578:Petroleum; D012989:Soil Pollutants; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D050557:Catechol 2,3-Dioxygenase