title: [Role of cell adhesion molecules Necl1 in synaptogenesis in primary cultured rat neurons].
abstract: OBJECTIVE
  To study the role of cell adhesion molecules Necl1 in synaptogenesis in primary cultured neurons.
  
  
  METHODS
  Semi-quantitive reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression pattern of Necl1 in the neuronal differentiation cell model in vitro. Western blot was performed to detect the expression pattern of Necl1 in primary cultured rat neurons and in purified synaptosome. Immunofluoresence was used to detect the synapse formation in primary neurons and in 293 cells co-culture and to detect the density of synapses in primary neuron with ectopic expression of Necl1.
  
  
  RESULTS
  Necl1 expression increased after retinoic acid (RA) induction in SH-SY5Y and P19 cells. The increase of Necl1 expression was consistent with the days of primary neurons culture in vitro, and Necl1 partly localized in synaptosome. The overexpression of Necl1 in 293 cells induced the synapse formation between cocultured 293 cells and neurons. Ectopic expression of Necl1 in primary neurons increased the density of synapses.
  
  
  CONCLUSIONS
  Necl1 plays an important role in neuronal synapse formation.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Hao
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.forename: Bo-Qin
authors.initials: BQ
authors.lastname: Qiang
authors.forename: Jian-Gang
authors.initials: JG
authors.lastname: Yuan
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Gong
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Peng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013569:Synapses / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; C516997:nectin-like molecule 1, rat; D014212:Tretinoin