Molecular characterization of rare bovine group A rotavirus G15P[11] and G15P[21] strains from eastern India: identification of simian SA11-like VP6 genes in G15P[21] strains.
During a surveillance study (November 2001-March 2005), one rare G15P[11] and two rare G15P[21] bovine group A rotavirus strains were detected in diarrhoeic calves in Eastern India. Sequence analysis of the VP8*, VP6, NSP4 and NSP5 genes of the G15P[11] strain confirmed its bovine origin. Although the NSP4 and NSP5 genes of the two G15P[21] strains were of bovine origin, their VP6 genes shared higher nucleotide and amino acid identities with simian strain SA11 (92.5-93.1% and 98.5-98.7%) than bovine strains (88.5-88.9% and 97-97.2%), and by phylogenetic analysis, exhibited clustering with SA11, distantly related to bovine strains. All these pointed towards a possible reassortment event of VP6 gene between bovine and simian (SA11-like) strains. Therefore, the present study provided molecular evidence for bovine origin of G15 strains and revealed a rare instance of genetic diversity in the bovine VP6 gene, otherwise conserved in group A rotavirus strains from cattle.
Ghosh
Souvik
S
Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme XM, Beliaghata, Kolkata, 700010, West Bengal, India.
Samajdar
Sudipta
S
Sinha
Manju
M
Kobayashi
Nobumichi
N
Taniguchi
Koki
K
Naik
Trailokya N
TN
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D017361:Viral Nonstructural Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*
D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins