2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter.
In a previous study, we examined the physiological responses of male Sprague-Dawley rats over a 4-week exposure to concrete and clay cages. No general toxicological changes were observed in rats exposed to either of the two cage types in summer. Under winter conditions, however, various general toxicological effects were detected in rats housed in concrete cages, although rats housed in clay cages showed no such effects. The infrared thermographic examination indicated that skin temperature in the concrete-housed rats was abnormally low, but not so in the clay-housed rats. We examined proteomic changes in the serum of rats housed in winter in concrete and clay cages using two-dimensional differential in-gel electrophoresis and mass spectrometry/mass spectrometry. Five proteins were identified and quantitatively validated; all were cold stress-induced, acute phase proteins that were either up-regulated (haptoglobin) or down-regulated (alpha-1-inhibitor III, alpha-2u globulin, complement component 3, and vitamin D-binding protein) in the concrete-housed rats. These results suggest that the 4-week exposure to a concrete cage in winter elicited a typical systemic inflammatory reaction (i.e. acute phase response) in the exposed rats.
Kim
Jong-Choon
JC
College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
Kim
Jin Young
JY
Yeom
Seok Ran
SR
Jeong
Bo Yoon
BY
Hwang
Hey-Zoo
HZ
Park
Keum-Joo
KJ
Lee
Seung-won
SW
D000209:Acute-Phase Proteins / Q000096:biosynthesis / Q000378:metabolism*; D000210:Acute-Phase Reaction / Q000097:blood / Q000503:physiopathology*; D000510:Alpha-Globulins / Q000096:biosynthesis; D000538:Aluminum Silicates; D000818:Animals; D001522:Behavior, Animal; D000077215:Clay; D003176:Complement C3 / Q000096:biosynthesis; D003252:Construction Materials / Q000009:adverse effects*; D015536:Down-Regulation; D004435:Eating; D015180:Electrophoresis, Gel, Two-Dimensional; D006242:Haptoglobins / Q000096:biosynthesis; D006799:Housing, Animal; D051381:Rats; D012621:Seasons; D012881:Skin Temperature; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation; D014809:Vitamin D-Binding Protein / Q000096:biosynthesis
D000209:Acute-Phase Proteins; D000510:Alpha-Globulins; D000538:Aluminum Silicates; D003176:Complement C3; D006242:Haptoglobins; D014809:Vitamin D-Binding Protein; C017894:alpha 1-inhibitor 3; C025500:alpha(2)-microglobulin; D000077215:Clay