Assessment of fibroblast cells submitted to ultrasonic irradiation.
Physiotherapists consider ultrasound an indispensable tool, which is commonly employed in clinical practice as a treatment aid for musculoskeletal dysfunctions. The aim of our study has been to analyze fibroblast cell structures following low-intensity pulsed ultrasonic irradiation. Fibroblast cell cultures irradiated with ultrasound were analyzed through electron microscopy to determine an ideal irradiation beam that preserved cell morphology and integrity. Analysis by fluorescence microscopy and transmission electron microscopy was used to follow morphological changes of the nucleus and cytoskeleton following different ultrasound irradiation intensities. According to the parameters used in the pulsed irradiation of fibroblast cultures, control over the intensity employed is fundamental to the optimal use of therapeutic ultrasound. Cell cultures submitted to low-intensity pulsed ultrasonic irradiation (0.2-0.6 W/cm2) at 10% (1:9 duty cycle) and 20% (2:8 duty cycle) maintained shape and cellular integrity, with little damage. In the group irradiated with an intensity of 0.8 W/cm2, a loss of adhesion was observed along with an alteration in the morphology of some cells at an intensity of 1.0 W/cm2, which resulted in the presence of cellular fragments and a decrease of adhering cells. In cells irradiated at 2.0 W/cm2, there was a complete loss of adhesion and aggregation of cellular fragments. The present study confirms that biophysical properties of pulsed ultrasound may accelerate proliferation processes in different biological tissues.
de Oliveira
Rodrigo Franco
RF
Laboratório de Dinâmica de Compartimento Celular, Instituto de Pesquisa e Desenvolvimento, IP&D, Univap, Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo, CEP 12244-000, Brazil.
Pires Oliveira
Deise A A
DA
Machado
Aline H A
AH
da Silva
Newton Soares
NS
Magini
Márcio
M
Pacheco-Soares
Cristina
C
D000818:Animals; D002460:Cell Line; D048430:Cell Shape; D005347:Fibroblasts / Q000000981:diagnostic imaging / Q000648:ultrastructure; D051379:Mice; D014465:Ultrasonics; D014463:Ultrasonography