title: Oligonucleotide nanostructured surfaces: effect on Escherichia coli curli expression.
abstract: Oligonucleotide model surfaces allowing independent variation of topography and chemical composition were designed to study the adhesion and biofilm growth of E.coli. Surfaces were produced by covalent binding of oligonucleotides and immobilization of nucleotide-based vesicles. Their properties were confirmed through a combination of fluorescence microscopy, XPS, ellipsometry, AFM and wettability studies at each step of the process. These surfaces were then used to study the response of three different strains of E.coli quantified in a static biofilm growth mode. This study led to convincing evidence that oligonucleotide-modified surfaces, independent of the topographical feature used in this study, enhanced curli expression without an increase in the number of adherent bacteria.
authors.affiliation: Institut de Chimie des Surfaces et Interfaces, CNRS UPR 9069, Mulhouse Cedex, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Cottenye
authors.forename: Francisco
authors.initials: F
authors.lastname: Teixeira
authors.forename: Arnaud
authors.initials: A
authors.lastname: Ponche
authors.forename: Günter
authors.initials: G
authors.lastname: Reiter
authors.forename: Karine
authors.initials: K
authors.lastname: Anselme
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Meier
authors.forename: Lydie
authors.initials: L
authors.lastname: Ploux
authors.forename: Corinne
authors.initials: C
authors.lastname: Vebert-Nardin
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms; D004926:Escherichia coli / Q000235:genetics*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D049329:Nanostructures; D009841:Oligonucleotides / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D009841:Oligonucleotides