[Biological transport of minocycline by human periodontal ligament fibroblasts].
OBJECTIVE
To investigate biological transport of minocycline by human periodontal ligament fibroblasts (HPDLF). To verify the hypothesis of delivering medicine to periodontium and the whole body through the root canal.


METHODS
HPDLF and MC3T3-E1 cells were incubated in minocycline solutions. The intracellular antibiotics contents were measured by high performance liquid chromatography (HPLC) and the cell total protein was measured by Bradford protein assay.


RESULTS
HPLC was an accurate, sensitive method for measurement of the intracellular minocycline. The incubation time and cell property had significant effect on the intracellular minocycline contents (P< 0.01). The intracellular contents increased with extracellular concentration.


CONCLUSIONS
Minocycline can be transported by HPDLF. The transport is concentration-dependent, time-dependent and cell specific.
Liu
Yu
Y
Dental Institute, General Hospital of PLA, Beijing 100853, China.
Liu
Hong-Chen
HC
Wu
Xia
X
E
Ling-Ling
LL
Leng
Bin
B
D000900:Anti-Bacterial Agents; D001692:Biological Transport; D002478:Cells, Cultured; D005347:Fibroblasts; D006801:Humans; D008911:Minocycline; D010513:Periodontal Ligament
D000900:Anti-Bacterial Agents; D008911:Minocycline