title: Repertoire and clonality of T-cell receptor beta variable genes expressed in endometrium and blood T cells of patients with recurrent spontaneous abortion.
abstract: OBJECTIVE
  Recurrent spontaneous abortion (RSA) is a relatively common disorder, the underlying causes of which are thought to be immunological in most cases.
  
  
  METHODS
  Expression profile and clonality pattern of T-cell receptor beta variable (TCRBV) genes in endometrium and blood of patients with RSA were investigated by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) using BV gene-specific primers. Relative expression of each BV family was determined and clonal expansion of the over-expressed genes was assessed by analysis of CDR3 length polymorphism.
  
  
  RESULTS
  Compared to blood, relative expression of four TCRBV genes was significantly higher in the endometrium of RSA group. Over-expressed genes, except for TCRBV3, all had restricted and oligoclonal patterns of expression in the endometrium.
  
  
  CONCLUSIONS
  Endometrial T cells have a skewed TCRBV repertoire with restricted transcript heterogeneity, which is shared by both groups and minor variations observed in this pattern in RSA patients may reflect more recent and/or repeated exposure to nominal antigens or superantigens.
authors.affiliation: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. pouneh_d@yahoo.com
authors.forename: Pouneh
authors.initials: P
authors.lastname: Dokouhaki
authors.forename: Rosa
authors.initials: R
authors.lastname: Moghaddam
authors.forename: MohammadReza
authors.initials: M
authors.lastname: Rezvany
authors.forename: Jamileh
authors.initials: J
authors.lastname: Ghassemi
authors.forename: Marefat Ghaffari
authors.initials: MG
authors.lastname: Novin
authors.forename: Amirhassan
authors.initials: A
authors.lastname: Zarnani
authors.forename: Mohammah-Mehdi
authors.initials: MM
authors.lastname: Akhondi
authors.forename: Mahyar
authors.initials: M
authors.lastname: Ostadkarampour
authors.forename: Håkan
authors.initials: H
authors.lastname: Mellstedt
authors.forename: Alireza
authors.initials: A
authors.lastname: Razavi
authors.forename: Mahmood
authors.initials: M
authors.lastname: Jeddi-Tehrani
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000328:Adult; D016022:Case-Control Studies; D022801:Complementarity Determining Regions / Q000235:genetics; D004717:Endometrium / Q000276:immunology* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D019674:Genes, T-Cell Receptor beta; D006801:Humans; D011110:Polymorphism, Genetic; D011247:Pregnancy; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta