Micropapillary carcinoma of the breast with necrosis-like cell death: a case report.
A primary invasive micropapillary carcinoma of the breast in a 46-year-old woman is reported. Histologically, it was composed predominantly of papillary tumor cell clusters without fibrovascular cores, surrounded by a clear space. Tumor cells were positive for cytokeratin (CK) 7, estrogen receptor (ER), and progesterone receptor (PR), but negative for p53, CK 20, CD34, c-Erb-B2, CK5, epidermal growth factor receptor (EGFR), vimentin, and c-kit. MUC1 expression was found at the reversed apical membrane of neoplastic cell clusters. Accordingly, electron microscopy showed the lack of basement membrane and presence of microvilli at the basal surface of the tumor cells. Moreover, ultrastructural examination revealed single tumor cell death characterized by patchy condensations of chromatin throughout the nucleus. These nuclear alterations were associated with the occurrence of empty cytoplasmic vacuoles, conferring a necrosis-like phenotype to this cell death. Alternative programmed cell deaths are reviewed and their morphologic distinction is discussed.
Caruso
Rosario Alberto
RA
Dipartimento di Patologia Umana, Policlinico Universitario, Messina, Italy. rosariocaruso@tin.it
Cicciarello
Rocco
R
Gagliardi
Maria Ester
ME
Albiero
Francesca
F
Costa
Gregorio
G
Fedele
Francesco
F
Cavaliere
Renato
R
Finocchiaro
Giuseppe
G
Mesiti
Mario
M
Cavallari
Vittorio
V
D014408:Biomarkers, Tumor / Q000032:analysis; D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000628:therapy; D002291:Carcinoma, Papillary / Q000737:chemistry / Q000473:pathology* / Q000628:therapy; D002467:Cell Nucleus / Q000648:ultrastructure; D003131:Combined Modality Therapy; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D053552:Keratin-7 / Q000032:analysis; D046529:Microscopy, Electron, Transmission; D008871:Microvilli / Q000648:ultrastructure; D008875:Middle Aged; D018396:Mucin-1 / Q000032:analysis; D009336:Necrosis; D011960:Receptors, Estrogen / Q000032:analysis; D014617:Vacuoles / Q000648:ultrastructure
D014408:Biomarkers, Tumor; D053552:Keratin-7; C060192:MUC1 protein, human; D018396:Mucin-1; D011960:Receptors, Estrogen