title: The biocompatibility of quantum dot probes used for the targeted imaging of hepatocellular carcinoma metastasis.
abstract: Semiconductor quantum dots (QDs) have several photo-physical advantages over organic dyes making them good markers in biomedical application. We used CdSe/ZnS QDs with maximum emission wavelength of 590nm (QD590) linked to alpha-fetoprotein (AFP) monoclonal antibody (Ab) to detect AFP in cytoplasm of human hepatocellular carcinoma (HCC) cell line HCCLM6. For the in vivo studies, we used QD-AFP-Ab probes for targeted imaging of human HCC xenograft growing in nude mice by injecting them into the tail vein. In addition, the cytotoxicity in vitro, the acute toxicity in vivo, the hemodynamics and tissue distribution of these probes were also investigated. The results in vitro and in vivo indicate that our QD-based probes have good stability, specificity and biocompatibility for ultrasensitive fluorescence imaging of molecular targets in our liver cancer model system.
authors.affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, No. 169 Donghu Road, Wuchang District, Wuhan 430071, China.
authors.forename: Liang-Dong
authors.initials: LD
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Yu
authors.forename: Man
authors.initials: M
authors.lastname: He
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Pei
authors.forename: Zhao-You
authors.initials: ZY
authors.lastname: Tang
authors.forename: Qu-Quan
authors.initials: QQ
authors.lastname: Wang
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology / Q000633:toxicity; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000503:physiopathology; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D006439:Hemodynamics / Q000187:drug effects; D006801:Humans; D021621:Imaging, Three-Dimensional; D008113:Liver Neoplasms / Q000473:pathology* / Q000503:physiopathology; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D015335:Molecular Probes / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology / Q000633:toxicity; D045663:Quantum Dots; D014018:Tissue Distribution / Q000187:drug effects; D023382:Toxicity Tests, Acute; D023041:Xenograft Model Antitumor Assays; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D015335:Molecular Probes; D000509:alpha-Fetoproteins