title: Differentiation of superficial-partial vs. deep-partial thickness burn injuries in vivo by confocal-laser-scanning microscopy.
abstract: OBJECTIVE
  The current determination of burn depth is based both on a visual and clinical assessment. Confocal-laser-scanning microscopy (CLSM) enables in vivo histomorphological images. We hypothesized that CLSM can differentiate superficial-partial vs. deep-partial thickness burns on a histomorphological level.
  
  
  METHODS
  Thirty-eight burn wounds in 14 patients were clinically divided in three groups from superficial (group 1), superficial-partial (group 2) to deep-partial (group 3) thickness burns. CLSM was performed with the Vivascope 1500 (Lucid Inc., Rochester, NY, USA) 24h after burn. The following parameters were assessed: cell size of the granular-layer, thickness of the basal-layer, minimal thickness of the epidermis and number of perfused dermal papillae.
  
  
  RESULTS
  Superficial burns resulted in a significant increase of the cell size of the granular-layer and a higher increase of the minimal thickness of the epidermis as in superficial-partial thickness burns. The granular-layer in partial thickness burns was destroyed. Superficial burns had an increased thickness of the basal-layer; in superficial-partial thickness burns the basal-layer was partly destroyed with complete destruction in deep-partial thickness burns. In superficial burns the perfused dermal papillae were increased significantly, while decreased in superficial-partial thickness, and completely destroyed in deep-partial thickness burns up to a depth of 350 microm.
  
  
  CONCLUSIONS
  In vivo confocal-laser-scanning microscopy can differentiate superficial-partial vs. deep-partial thickness burns on a histomorphological level.
authors.affiliation: Department of Plastic, Hand and Reconstructive Surgery, Burn Centre, Medizinische Hochschule Hannover, Hannover, Germany. dr.med.altintas@gmail.com
authors.forename: M A
authors.initials: MA
authors.lastname: Altintas
authors.forename: A A
authors.initials: AA
authors.lastname: Altintas
authors.forename: K
authors.initials: K
authors.lastname: Knobloch
authors.forename: M
authors.initials: M
authors.lastname: Guggenheim
authors.forename: C J
authors.initials: CJ
authors.lastname: Zweifel
authors.forename: P M
authors.initials: PM
authors.lastname: Vogt
mesh_terms: D002056:Burns / Q000473:pathology*; D048429:Cell Size; D004817:Epidermis / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D015599:Trauma Severity Indices; D014945:Wound Healing / Q000502:physiology