Development of microsatellite markers and construction of genetic map in rice blast pathogen Magnaporthe grisea.
Magnaporthe grisea is the most destructive fungal pathogen of rice and a model organism for studying plant-pathogen interaction. Molecular markers and genetic maps are useful tools for genetic studies. In this study, based on the released genome sequence data of M. grisea, we investigated 446 simple sequence repeat (SSR) loci and developed 313 SSR markers, which showed polymorphisms among nine isolates from rice (including a laboratory strain 2539). The number of alleles of each marker ranged 2-9 with an average of 3.3. The polymorphic information content (PIC) of each marker ranged 0.20-0.89 with an average of 0.53. Using a population derived from a cross between isolates Guy11 and 2539, we constructed a genetic map of M. grisea consisting of 176 SSR markers. The map covers a total length of 1247 cM, equivalent to a physical length of about 35.0 Mb or 93% of the genome, with an average distance of 7.1cM between adjacent markers. A web-based database of the SSR markers and the genetic map was established (http://ibi.zju.edu.cn/pgl/MGM/index.html).
Zheng
Yan
Y
Department of Agronomy, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, China.
Zhang
Geng
G
Lin
Fucheng
F
Wang
Zonghua
Z
Jin
Gulei
G
Yang
Long
L
Wang
Ying
Y
Chen
Xi
X
Xu
Zhaohua
Z
Zhao
Xiangqian
X
Wang
Hongkai
H
Lu
Jianping
J
Lu
Guodong
G
Wu
Weiren
W
D030541:Databases, Genetic; D008040:Genetic Linkage; D020082:Magnaporthe / Q000235:genetics*; D018895:Microsatellite Repeats; D012275:Oryza / Q000382:microbiology*; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000382:microbiology*; D011110:Polymorphism, Genetic