title: [Sequence analysis of a novel human leukocyte antigen allele HLA-A*9206].
abstract: OBJECTIVE
  To investigate the molecular genetic basis for a human leukocyte antigen (HLA) novel allele HLA-A*9206 in the Chinese population.
  
  
  METHODS
  DNA was extracted from whole blood by PEL-FREEZ DNA extraction kit. The amplification of HLA-A exons 1-8 of the proband was preformed and the PCR products were sequenced using ABI sequencing kit. Both strands of exons 2, 3 and 4 of the amplified product were sequenced. The polymerase chain reaction-sequence specific primer (PCR-SSP) was performed to split the two alleles apart and confirm the mutations detected by sequencing.
  
  
  RESULTS
  The sequencing results showed that the HLA-A alleles of the proband were A*1101 and a novel allele. The sequence of the novel allele has been submitted to GenBank (EF062306). After Blast analysis, the novel allele shows one nucleotide different from the HLA-A*0206 in exon 3 at nucleotide position 530 (C to T). This results in an amino acid change from Ala to Val at codon 153.
  
  
  CONCLUSIONS
  This allele is a novel allele and has been officially named A*9206 by the WHO Nomenclature Committee.
authors.affiliation: Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou, Zhejiang, 310006 People's Republic of China.
authors.forename: Jun-jun
authors.initials: JJ
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhe-dong
authors.initials: ZD
authors.lastname: Han
authors.forename: Nan-ying
authors.initials: NY
authors.lastname: Chen
authors.forename: Fa-ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Li-xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D000483:Alleles; D044466:Asian People; D001483:Base Sequence; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015234:HLA-A Antigens