title: Identification of differentially expressed genes in rat silicosis model by suppression subtractive hybridization analysis.
abstract: The critical molecular mechanism in the development of the pulmonary fibrosis remains unknown, leaving diagnosed patients with a poor prognosis. To isolate the genes specifically up-regulated in pulmonary fibrosis, we established a rat silicosis model 360 d after treatment with crystalline silica suspension. Radiographs of chests showed that some scattered high-density shadows appeared in the lung field. Typical microscopic fibrosing silicotic nodules formed in the lung, alveolar epithelial cells and bronchial epithelial cells, particularly around the partial fibrosing silicotic nodules; some of them showed atypical hyperplasia that suggested a correlation between silicosis and lung cancer. Suppression subtractive hybridization analysis was performed to compare gene expression in lung tissue with silicosis and normal lung tissue. Reverse transcription-polymerase chain reaction showed that the expressions of seven novel cDNA sequences identified by suppression subtractive hybridization in lung tissue with silicosis differed from normal lung tissue. Bioinformatics analysis showed that 47 positive clones represented 35 genes containing two putative proteins and four predicted similar proteins. The analysis also showed that some screened genes in silicosis, such as prolyl 4-hydroxylases, actin-related protein-2/3 complex and acidic mammalian chitinase, have not been previously reported. These genes may provide new clues for investigating the molecular mechanisms in the development of pulmonary fibrosis.
authors.affiliation: Department of Pathology, Xiangya Medical School, Central South University, Changsha 410013, China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Jin
authors.forename: Baoan
authors.initials: B
authors.lastname: Liu
authors.forename: Deyun
authors.initials: D
authors.lastname: Feng
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Hu
authors.forename: Jinwu
authors.initials: J
authors.lastname: Peng
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Du
authors.forename: Chunyan
authors.initials: C
authors.lastname: Fu
authors.forename: Jifang
authors.initials: J
authors.lastname: Wen
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology; D009693:Nucleic Acid Hybridization; D011658:Pulmonary Fibrosis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012829:Silicosis / Q000235:genetics* / Q000473:pathology
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary