Applications of next-generation sequencing technologies in functional genomics.
A new generation of sequencing technologies, from Illumina/Solexa, ABI/SOLiD, 454/Roche, and Helicos, has provided unprecedented opportunities for high-throughput functional genomic research. To date, these technologies have been applied in a variety of contexts, including whole-genome sequencing, targeted resequencing, discovery of transcription factor binding sites, and noncoding RNA expression profiling. This review discusses applications of next-generation sequencing technologies in functional genomics research and highlights the transforming potential these technologies offer.
Morozova
Olena
O
BC Cancer Agency Genome Sciences Centre, Vancouver, BC, Canada.
Marra
Marco A
MA
D001709:Biotechnology / Q000379:methods*; D019295:Computational Biology / Q000379:methods*; D020869:Gene Expression Profiling; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*