The novel protein complex with SMARCAD1/KIAA1122 binds to the vicinity of TSS.
The SMARCAD1/KIAA1122 protein is structurally classified into the SWI2/SNF2 superfamily of DNA-dependent ATPases that are catalytic subunits of chromatin-remodeling complexes. Although the importance of other members of the SWR1-like subfamily in chromatin remodeling (EP400, INOC1, and SRCAP) has already been elucidated, the biological function of SMARCAD1/KIAA1122 in transcriptional regulation remains to be clarified. To gain insight into the role of this protein, we generated a specific antibody against SMARCAD1/KIAA1122 and used it for chromatin and protein immunoprecipitation assays. We employed high-resolution genome tiling microarrays in chromatin immunoprecipitation and found the binding sites of SMARCAD1/KIAA1122 in the vicinity of the transcriptional start site of 69 candidate target genes. In the protein immunoprecipitation assay, we found that endogenous SMARCAD1/KIAA1122 binds with TRIM28, a recently highlighted transcriptional regulator in the cancer field. From these findings, we propose a novel model for gene regulation via the SMARCAD1/KIAA1122 protein complex.
Okazaki
Noriko
N
Laboratory of Medical Genomics, Department of Human Genome Research, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
Ikeda
Shun
S
Ohara
Reiko
R
Shimada
Kiyo
K
Yanagawa
Toshihide
T
Nagase
Takahiro
T
Ohara
Osamu
O
Koga
Hisashi
H
D000595:Amino Acid Sequence; D000818:Animals; D004265:DNA Helicases; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D009373:Neoplasms, Germ Cell and Embryonal / Q000378:metabolism / Q000473:pathology; D009687:Nuclear Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D011485:Protein Binding; D024363:Transcription Initiation Site
D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D004265:DNA Helicases; C079429:Smarcad1 protein, mouse