Effects of hydroxytyrosol and hydroxytyrosol acetate administration to rats on platelet function compared to acetylsalicylic acid.
Virgin olive oil (VOO) contains the polyphenols hydroxytyrosol (HT) and hydroxytyrosol acetate (HT-AC). This study investigated the antiplatelet effect of HT and HT-AC in healthy rats and compared their effects to acetylsalicylic acid (ASA). All compounds were administered orally for 7 days. HT and HT-AC inhibited platelet aggregation in whole blood, with a 50% inhibitory dose (ID50) of 48.25 mg/kg per day for HT, 16.05 mg/kg per day for HT-AC, and 2.42 mg/kg per day for ASA. Platelet synthesis of thromboxane B2 was inhibited by up to 30% by HT and 37% by HT-AC; the ID50 of this effect for ASA was 1.09 mg/kg per day. Vascular prostacyclin production was inhibited by up to 27.5% by HT and 32% by HT-AC; the ID50 of this effect for ASA was 6.75 mg/kg per day. Vascular nitric oxide production was increased by up to 34.2% by HT, 66% by HT-AC, and 64% by ASA. We conclude that HT and HT-AC administered orally inhibited platelet aggregation in rats and that a decrease in thromboxane synthesis along with an increase in nitric oxide production contributed to this effect.
González-Correa
José Antonio
JA
Laboratorio de Investigaciones Antitrombóticas e Isquemia Tisular, Department of Pharmacology, School of Medicine, University of Malaga, 39071 Malaga, Spain.
Navas
María Dolores
MD
Muñoz-Marín
Javier
J
Trujillo
Mariana
M
Fernández-Bolaños
Juan
J
de la Cruz
José Pedro
JP
D000085:Acetates / Q000008:administration & dosage*; D000818:Animals; D001241:Aspirin / Q000008:administration & dosage*; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology*; D002396:Catechols / Q000008:administration & dosage*; D003094:Collagen / Q000494:pharmacology; D015777:Eicosanoids / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
D000085:Acetates; D002396:Catechols; D015777:Eicosanoids; D010975:Platelet Aggregation Inhibitors; C524604:hydroxytyrosyl acetate; C005975:3,4-dihydroxyphenylethanol; D009569:Nitric Oxide; D003094:Collagen; D010626:Phenylethyl Alcohol; D001241:Aspirin