title: Placental Alpha Hemoglobin Stabilizing Protein (AHSP) and recurrent miscarriage.
abstract: AHSP inhibits cellular production of the reactive oxygen species. Reduced AHSP indicates reduced protection against oxidative stressors. Our objective was to investigate AHSP levels in recurrent miscarriage (RM). Trophoblast was collected from women of 10 weeks gestation: voluntary abortion controls (VA, n = 10); spontaneous first miscarriage with subsequent normal pregnancy (SMSN, n = 15) or with subsequent miscarriage (SMSM, n = 5); RM previously investigated (RMPS, n = 5) or not previously investigated (RM, n = 5). AHSP mRNA and protein were determined using real-time quantitative polymerase chain reaction (PCR) and Western blot, respectively. One-way ANOVA was performed to assess statistical significance (p < 0.05). ahsp mRNA levels were maximally reduced in RM and RMPS (8.0 x 10(-6) +/- 1.3 and 8.1 x 10(-6) +/- 0.7, respectively) compared with SMSN and VA (16.1 x 10(-6) +/- 2.3 and 26.1 x 10(-6) +/- 2.7, respectively). SMSM showed levels significantly reduced as well (9.0 x 10(-6) +/- 2.3). In RM, a reduced defense from oxidative stressors is evident at first miscarriage, identifying women at high risk for subsequent eventful pregnancy. Reduced AHSP may identify women at risk of experiencing further miscarriages.
authors.affiliation: Institute for Biochemical Biotechnologies, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Emanuelli
authors.forename: Monia
authors.initials: M
authors.lastname: Cecati
authors.forename: Davide
authors.initials: D
authors.lastname: Sartini
authors.forename: Piergiorgio
authors.initials: P
authors.lastname: Stortoni
authors.forename: Alessandra
authors.initials: A
authors.lastname: Corradetti
authors.forename: Stefano R
authors.initials: SR
authors.lastname: Giannubilo
authors.forename: Angelo
authors.initials: A
authors.lastname: Turi
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Tranquilli
mesh_terms: D000026:Abortion, Habitual / Q000378:metabolism*; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C468768:AHSP protein, human; D001798:Blood Proteins; D018832:Molecular Chaperones; D012333:RNA, Messenger