Genotypic heterogeneity among strains of Mycoplasma gallinarum.
Seven field strains of Mycoplasma gallinarum isolated from chickens on three local farms and the type strain PG 16 were compared by restriction endomiclease DNA analysis using EcoRI, HindIII, BamHI, PstI and Xhol. The restriction patterns generated by the first four enzymes indicated considerable DNA polymorphisms among M. gallinarum strains. Field strains revealed 3 distinct patterns of the genomic DNA which differed from that of the PG 16 strain. BamHI enabled easy distinguishing between related and unrelated strains due to the fragments being characteristic for certain strains. Interestingly, the characteristic patterns were associated with the corresponding chicken selection and this suggests that DNA analysis has a potential use for studying the epidemiology of M. gallinarum.
Dovc
P
P
Zootechnical Department, Biotechnical Faculty, University of Ljubljana, Domzale, Slovenia, Yugoslavia.
Bencina
D
D
Zajc
I
I