title: Inter- and intra-assay reproducibility of microplate Alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis.
abstract: The intersample and intrasample variability of the results obtained with the microplate Alamar blue assay for the indirect drug susceptibility testing of Mycobacterium tuberculosis was investigated. Between 1.2 and 8.5% of paired MICs differed by more than one twofold dilution, resulting in discordant susceptible-resistant designations at frequencies between 0.6% (rifampin) and 18.9% (ethambutol).
authors.affiliation: Laboratorio de Investigación de Enfermedades Infecciosas, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado No. 430, San Martín de Porras, Lima 31, Perú.
authors.forename: Brian
authors.initials: B
authors.lastname: Leonard
authors.forename: Jorge
authors.initials: J
authors.lastname: Coronel
authors.forename: Mark
authors.initials: M
authors.lastname: Siedner
authors.forename: Louis
authors.initials: L
authors.lastname: Grandjean
authors.forename: Luz
authors.initials: L
authors.lastname: Caviedes
authors.forename: Pilar
authors.initials: P
authors.lastname: Navarro
authors.forename: Robert H
authors.initials: RH
authors.lastname: Gilman
authors.forename: David A J
authors.initials: DA
authors.lastname: Moore
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D010078:Oxazines / Q000378:metabolism; D015203:Reproducibility of Results; D014966:Xanthenes / Q000378:metabolism
chemical_list: D000995:Antitubercular Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin