Transgenic tobacco plants overexpressing the Nicta; CycD3; 4 gene demonstrate accelerated growth rates.
D-type cyclins control the onset of cell division and the response to extracellular signals during the G1 phase. In this study, we transformed a D-type cyclin gene, Nicta;CycD3;4, from Nicotiana tabacum using an Agrobacterium-mediated method. A predicted 1.1 kb cyclin gene was present in all of the transgenic plants, but not in wild-type. Northern analyses showed that the expression level of the Nicta;CycD3;4 gene in all of the transgenic plants was strong when compared to the wild-type plants, suggesting that Nicta;CycD3;4 gene driven by the CaMV 35S promoter was being overexpressed. Our results revealed that transgenic plants overexpressing Nicta;CycD3;4 had an accelerated growth rate when compared to wild-type plants, and that the transgenic plants exhibited a smaller cell size and a decreased cell population in young leaves when compared to wild-type plants.
Guo
Jia
J
School of Biotechnology, Kangwon National University, Chuncheon, Korea.
Wang
Myeong Hyeon
MH
D003001:Cloning, Molecular; D056743:Cyclin D3; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics*; D016213:Cyclins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development*; D015854:Up-Regulation
D056743:Cyclin D3; D016213:Cyclins; D051358:Cyclin-Dependent Kinase 4