title: Cloning and characterization of myosin regulatory light chain (MRLC) gene from Culex pipiens pallens.
abstract: Myosin regulatory light chain (MRLC) (GenBank accession no. DQ140391) was cloned from Culex pipiens pallens. An open reading frame (ORF) of 630 bps was found to encode a putative 210 amino acids protein which shows 73% similarity with myosin regulatory light chain of Gryllotalpa orientalis. Real-time quantitative PCR analysis demonstrated that the transcription level of MRLC in deltamethrin-resistant strain (DR-strain) was 4.08-fold higher than in deltamethrin-susceptible strain (DS-strain) of C. pipiens pallens. Over-expression of MRLC in Aedes albopictus C6/36 cells conferred protection against deltamethrin based on tritiated methyl tritiated thymidine ((3)H-TdR) incorporation assay. These results indicate that MRLC may be a potential cause of deltamethrin resistance in C. pipiens pallens.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 210029, PR China.
authors.forename: Mifang
authors.initials: M
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Changliang
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology; D008969:Molecular Sequence Data; D018994:Myosin Light Chains / Q000235:genetics*; D009570:Nitriles / Q000494:pharmacology; D010802:Phylogeny; D011722:Pyrethrins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D007306:Insecticides; D018994:Myosin Light Chains; D009570:Nitriles; D011722:Pyrethrins; D011994:Recombinant Proteins; C017180:decamethrin