title: Reinforcement of porous alginate scaffolds by incorporating electrospun fibres.
abstract: The mechanical properties of scaffolds play a vital role in transmitting input mechanical signals to the cells within them. We aimed to modify mechanical properties of porous scaffolds by incorporating electrospun fibres into their frameworks. Porous constructs containing electrospun silicate fibres were prepared from Na-alginate aqueous solutions suspending the silicate fibres with (ASF) or without amino groups (NASF) via an all-aqueous method based on a freeze-drying technique. The repulsion forces of constructs containing ASF towards compression increased as the fibre content increased. In contrast, constructs containing NASF showed no such increases in repulsion forces. Cells seeded onto constructs containing ASF exhibited suppressed growth, similar to cells seeded onto alginate scaffolds without fibres. In contrast, cells seeded onto scaffolds containing NASF showed about two-fold faster growth than cells seeded onto scaffolds containing ASF. The differences in the mechanical properties and cell growth profiles between the scaffolds containing ASF and NASF can be explained by the formation and non-formation of electrostatic bonds between the fibres and alginate, respectively. The results obtained in the present study demonstrate the feasibility of incorporating electrospun fibres for reinforcement of alginate scaffolds and enhancement of cell growth.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Takagi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yamada
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Koei
authors.initials: K
authors.lastname: Kawakami
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D004563:Electrochemistry / Q000379:methods; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D016062:Porosity; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020723:Glucuronic Acid