[Chemical characteristics and endotoxic activity of the lipopolysaccharide of Rahnella aquatilis 2-95].
The lipopolysaccharide (LPS) from a new Enterobacteriaceae species, Rahnella aquatilis 2-95, was isolated and investigated. The structural components of the LPS molecule, namely, lipid A, core oligosaccharide, and O-specific polysaccharide, were obtained by mild acid hydrolysis. In lipid A, 3-oxytetradecanoic and tetradecanoic acids were found to be the predominant fatty acids. The major monosaccharides of the core oligosaccharide were galactose, arabinose, fucose, rhamnose, and an unidentified component. The O-specific polysaccharide was found to be assembled of a repeated trisaccharide unit of the following structure: [structure: see text]. The R. aquatilis 2-95 LPS is less toxic and more pyrogenic as compared to the one from the R. aquatilis 1-95 strain studied earlier. Both acyl and phosphate groups are essential for toxic and pyrogenic activity of R. aquatilis 2-95 LPS.
Varbanets
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Zdorovenko
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Ostapchuk
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D000818:Animals; D004731:Endotoxins / Q000737:chemistry; D006868:Hydrolysis; D007928:Lethal Dose 50; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D051379:Mice; D019081:O Antigens / Q000737:chemistry; D011749:Pyrogens / Q000633:toxicity; D011817:Rabbits; D020638:Rahnella / Q000737:chemistry*
D004731:Endotoxins; D008050:Lipid A; D008070:Lipopolysaccharides; D019081:O Antigens; D011749:Pyrogens; C042058:core endotoxin