Antioxidant responses in different body regions of the polychaeta Laeonereis acuta (Nereididae) exposed to copper.
Antioxidant enzymes, total antioxidant capacity (TOSC) and concentration of reactive oxygen species (ROS) were measured in anterior (A), middle (M) and posterior (P) body regions of Laeonereis acuta after copper (Cu; 62.5 microg/l) exposure. A catalase (CAT) activity gradient observed in control group (lowest in A, highest in P) was not observed in Cu exposed group. Glutathione-S-transferase (GST) activity in A region of Cu group was higher than in A region of the control group. DNA damage (comet assay) was augmented in the A region of Cu group. Since copper accumulation was similar in the different body regions, sensitivity to copper in A regions seems to be related to lowest CAT activity. In sum, copper exposure lowered TOSC, a result that at least in part can be related to lowering of antioxidant enzymes like CAT. DNA damage was induced in the anterior region, where a lower CAT activity was observed.
Ferreira-Cravo
Marlize
M
Curso de Pós-graduação em Ciências Fisiológicas-Fisiologia Animal Comparada (FURG), Brazil.
Ventura-Lima
Juliane
J
Sandrini
Juliana Zomer
JZ
Amado
Lílian Lund
LL
Geracitano
Laura Alicia
LA
Rebelo
Mauro
M
Bianchini
Adalto
A
Monserrat
José Maria
JM
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D002374:Catalase / Q000032:analysis / Q000378:metabolism; D020552:Comet Assay; D003300:Copper / Q000633:toxicity*; D004249:DNA Damage; D005982:Glutathione Transferase / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D011077:Polychaeta / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D003300:Copper; D002374:Catalase; D005982:Glutathione Transferase