title: Environmental cues from CNS, PNS, and ENS cells regulate CNS progenitor differentiation.
abstract: Cellular origin and environmental cues regulate stem cell fate determination. Neuroepithelial stem cells form the central nervous system (CNS), whereas neural crest stem cells generate the peripheral (PNS) and enteric nervous system (ENS). CNS neural stem/progenitor cell (NSPC) fate determination was investigated in combination with dissociated cultures or conditioned media from CNS, PNS, or ENS. Cells or media from ENS or PNS cultures efficiently promoted NSPC differentiation into neurons, glia, and smooth muscle cells with a similar morphology as the feeder culture. Together with CNS cells or its conditioned medium, NSPC differentiation was partly inhibited and cells remained immature. Here, we demonstrate that secreted factors from the environment can influence CNS progenitor cells to choose a PNS-like cell fate.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University Biomedical Center, Uppsala, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Brännvall
authors.forename: Mikael
authors.initials: M
authors.lastname: Corell
authors.forename: Karin
authors.initials: K
authors.lastname: Forsberg-Nilsson
authors.forename: Asa
authors.initials: A
authors.lastname: Fex Svenningsen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D017615:Enteric Nervous System / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009414:Nerve Growth Factors / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D017933:Peripheral Nervous System / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D050819:Transcription Factor Brn-3A / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; C494189:Pou4f1 protein, mouse; D050819:Transcription Factor Brn-3A; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins