A multiplexed protein microarray for the simultaneous serodiagnosis of human immunodeficiency virus/hepatitis C virus infection and typing of whole blood.
All donor blood samples must be tested pretransfusion to determine the donor blood type. Standard testing protocols require that assays be performed for important bloodborne pathogens such as hepatitis C, syphilis, hepatitis B, and human immunodeficiency virus. We have demonstrated proof of the concept that a protein microarray can type whole blood and detect antibody to significant pathogens simultaneously from the same donor blood sample. The data collected demonstrate the ability of the array to accurately type blood samples while also detecting the presence of antibodies against both human immunodeficiency virus and hepatitis C virus. In conclusion, we have successfully developed a platform capable of typing human whole blood samples, while at the same time testing for the presence of antibodies specific for human immunodeficiency virus/hepatitis C virus. The major benefits of this system are its amenability to expansion with additional assays, for example, rhesus typing and syphilis and/or hepatitis B virus detection, and also the adaptability of the assay to higher-throughput analysis, currently 16 individual samples per slide, but readily expandable to a 96-well format.
Burgess
Stewart T G
ST
Division of Pathway Medicine, College of Medicine and Veterinary Medicine, The University of Edinburgh, Edinburgh, UK.
Kenyon
Fiona
F
O'Looney
Nichola
N
Ross
Alan J
AJ
Chong Kwan
Marisa
M
Beattie
John S
JS
Petrik
Juraj
J
Ghazal
Peter
P
Campbell
Colin J
CJ
D000818:Animals; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D002417:Cattle; D003429:Cross Reactions; D015483:HIV Antibodies / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology; D015658:HIV Infections / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000276:immunology; D006526:Hepatitis C / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology*; D018936:Hepatitis C Antigens / Q000276:immunology; D006801:Humans; D010641:Phenotype; D040081:Protein Array Analysis; D012698:Serologic Tests / Q000379:methods*; D013997:Time Factors
D015483:HIV Antibodies; D015488:HIV Antigens; D006508:Hepatitis Antibodies; D018936:Hepatitis C Antigens