title: The content of bone morphogenetic proteins in platelets varies greatly between different platelet donors.
abstract: Platelet derivates and platelet rich plasma have been used to stimulate bone formation and wound healing because of the rich content of potent growth factors. However, not all reports have been conclusive since some have not been able to demonstrate a positive effect. We investigated the interindividual variation of bone morphogenetic proteins (BMPs) in platelets from healthy donors, and the pH-dependent effect on the release of BMPs in preparations of lysed platelets in buffer (LPB). Platelet concentrates from 31 healthy donors were prepared in pH 4.3 and pH 7.4 buffers and investigated with respect to BMP-2, -4, -6, and -7. BMP-2 and BMP-4 were significantly more common in acidic LPBs in comparison with neutral preparations. We also observed a considerable variation among platelet donors with respect to the release of BMPs at pH 4.3 and 7.4. In conclusion, a considerable variation was found among platelet donors, which may be of importance considering the ambiguous results previously reported on osteoblast proliferation and differentiation.
authors.affiliation: Division of Orthopaedics, Department of Clinical and Experimental Medicine, Faculty of Health Sciences at Linköping University, SE-581 85 Linköping, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Kalén
authors.forename: Ola
authors.initials: O
authors.lastname: Wahlström
authors.forename: Cecilia Halling
authors.initials: CH
authors.lastname: Linder
authors.forename: Per
authors.initials: P
authors.lastname: Magnusson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001782:Blood Donors; D001792:Blood Platelets / Q000378:metabolism*; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology
chemical_list: D019485:Bone Morphogenetic Proteins