title: Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues.
abstract: We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3%) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsatellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and progression of GBMs.
authors.affiliation: Department of Neurosurgery, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kuga
authors.forename: Masahiro
authors.initials: M
authors.lastname: Mizoguchi
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Guan
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hata
authors.forename: Koji
authors.initials: K
authors.lastname: Yoshimoto
authors.forename: Tadahisa
authors.initials: T
authors.lastname: Shono
authors.forename: Satoshi O
authors.initials: SO
authors.lastname: Suzuki
authors.forename: Yoji
authors.initials: Y
authors.lastname: Kukita
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tahira
authors.forename: Shinji
authors.initials: S
authors.lastname: Nagata
authors.forename: Tomio
authors.initials: T
authors.lastname: Sasaki
authors.forename: Kenshi
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics*; D002648:Child; D005260:Female; D018628:Gene Dosage; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007834:Lasers; D019656:Loss of Heterozygosity; D008297:Male; D042282:Microdissection; D018895:Microsatellite Repeats; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction