title: FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy.
abstract: Cajal Bodies are one of many specialised organelles contained in the eukaryotic cell nucleus, and are involved in a number of functions, including regulation of replication-dependent histone gene transcription. In normal diploid cells their number varies between 0 and 4 depending on the cell cycle phase, although in cancer cell lines their number is extremely variable and it has been suggested that it correlates with cell ploidy. Here we show that in mammalian cells, as in Drosophila, two distinct though functionally related bodies exist: a histone gene locus body and a Cajal Body. The first one can be detected using FLASH or NPAT as markers while the second is labelled using antibodies against Coilin. Only the number of FLASH/NPAT histone gene locus bodies correlates with ploidy and only these organelles appear to be regulated during the cell cycle. Finally, we show that the two organelles completely co-localize during the S phase of the cell cycle.
authors.affiliation: Department of Experimental Medicine, University of Rome Tor Vergata, Roma, Italy.
authors.forename: Lucilla
authors.initials: L
authors.lastname: Bongiorno-Borbone
authors.forename: Antonella
authors.initials: A
authors.lastname: De Cola
authors.forename: Patrizia
authors.initials: P
authors.lastname: Vernole
authors.forename: Livio
authors.initials: L
authors.lastname: Finos
authors.forename: Daniela
authors.initials: D
authors.lastname: Barcaroli
authors.forename: Richard A
authors.initials: RA
authors.lastname: Knight
authors.forename: Gerry
authors.initials: G
authors.lastname: Melino
authors.forename: Vincenzo
authors.initials: V
authors.lastname: De Laurenzi
mesh_terms: D000782:Aneuploidy; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D020541:Coiled Bodies / Q000378:metabolism*; D020732:Cytogenetic Analysis; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D011003:Ploidies
chemical_list: D051017:Apoptosis Regulatory Proteins; C485604:CASP8AP2 protein, human; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; C101693:NPAT protein, human; D009687:Nuclear Proteins; C068921:p80-coilin