A comparison of muscle- and scale-derived delta13C and delta15N across three life-history stages of Atlantic salmon, Salmo salar.
Stable isotope signatures were obtained from paired scale and muscle tissue samples from smolt, post-smolt and one-sea-winter adult Atlantic salmon (Salmo salar). Post-smolt and adult scales were separated into central and outer (marine) portions with analyses carried out on the marine growth section of both life-history stages and the central portion for the adult scales. Muscle and scale delta(13)C and delta(15)N signatures were assessed (1) to determine whether a linear relationship exists between tissue types, (2) to determine if a constant offset exists between tissue signatures across all life-history stages, and (3) to evaluate whether underplating imparts a significant bias to life-history scale segments that would preclude their use in retrospective analyses of any ontogenetic dietary changes between life-history stages. Significant correlations were found to exist between muscle and scale stable isotope signatures obtained from smolts (delta(13)C and delta(15)N) and adults (delta(15)N). Both the muscle and the scale signatures captured the dietary shift associated with the transition from freshwater to the marine environment. Post-smolt and adult scales were depleted relative to muscle tissue, which may be attributed to isotopic differences in amino acid composition between muscle and scale tissues. The results suggest that scales may better represent dietary carbon sources because they are not influenced by lipid dynamics. The scale, however, appears less responsive to short-term shifts in diet relative to muscle and, therefore, must be used only to infer seasonally integrated dietary patterns for slow-growing life-history stages.
Sinnatamby
R Niloshini
RN
Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada. nsinnata@scimail.uwaterloo.ca
Dempson
J Brian
JB
Power
Michael
M
D000375:Aging / Q000502:physiology*; D000818:Animals; D002247:Carbon Isotopes / Q000032:analysis*; D008018:Life Cycle Stages / Q000502:physiology*; D013058:Mass Spectrometry / Q000379:methods; D018482:Muscle, Skeletal / Q000737:chemistry*; D009587:Nitrogen Isotopes / Q000032:analysis*; D012474:Salmon / Q000502:physiology*; D012867:Skin / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes