Capillary electrophoresis chips for screening of endotoxin chemotypes from whole-cell lysates.
A fast microchip electrophoresis method was developed to analyze and differentiate bacterial endotoxins directly from whole-cell lysates after removal of the proteinaceous components with proteinase K digestion and a precipitation of the endotoxin components. The partially purified endotoxin components were visualized by the interaction with dodecyl sulphate and then a fluorescent dye. The lipopolysaccharide (LPS) profiles can be directly evaluated from digested bacterial cells, and the electrophoresis patterns very closely resembled to those of pure LPSs, and the R and S chemotypes can be used to assign the strains. The method has been found to be useful in the screening of a large number of bacterial mutants and the structural characterization of endotoxins extracted only from 1 ml cultures.
Kilár
Anikó
A
Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti út 12, 7624 Pécs, Hungary.
Péterfi
Zoltán
Z
Csorba
Eszter
E
Kilár
Ferenc
F
Kocsis
Béla
B
D046209:Electrophoresis, Microchip / Q000379:methods*; D004755:Enterobacteriaceae / Q000737:chemistry* / Q000145:classification; D008070:Lipopolysaccharides / Q000032:analysis* / Q000145:classification; D012703:Serotyping / Q000379:methods*
D008070:Lipopolysaccharides