title: Intrafollicular antimüllerian hormone levels predict follicle responsiveness to follicle-stimulating hormone (FSH) in normoandrogenic ovulatory women undergoing gonadotropin releasing-hormone analog/recombinant human FSH therapy for in vitro fertilization and embryo transfer.
abstract: OBJECTIVE
  To investigate the relationship between antimüllerian hormone (AMH) and steroidogenesis in follicles of normoandrogenic ovulatory women undergoing follicle-stimulating hormone (FSH) therapy for in vitro fertilization/embryo transfer (IVF-ET).
  
  
  METHODS
  Prospective cohort.
  
  
  METHODS
  Institutional/private practice.
  
  
  METHODS
  26 normoandrogenic ovulatory women. All women received gonadotropin-releasing hormone (GnRH) analog and ovarian stimulation for IVF-ET.
  
  
  METHODS
  Follicle fluid was aspirated at oocyte retrieval from the first follicle of each ovary.
  
  
  METHODS
  Follicle fluid was assayed for AMH, estradiol (E(2)), progesterone, androstenedione, testosterone, dihydrotestosterone, insulin, and FSH.
  
  
  RESULTS
  Intrafollicular AMH levels positively and negatively correlated with E(2) and FSH concentrations in follicles, respectively, causing a positive relationship between follicle fluid AMH levels and E(2)/FSH ratios as a measure of follicle sensitivity to FSH. A positive relationship also existed in follicles between AMH levels and E(2)/androgen ratios as a marker of aromatase activity.
  
  
  CONCLUSIONS
  The AMH levels in follicles of IVF patients positively correlate with follicle sensitivity to FSH.
authors.affiliation: Reproductive Medicine and Infertility Associates, 2101 Woodwinds Drive, Woodbury, MN 55125, USA. danieldumesic@aol.com
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Dumesic
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Lesnick
authors.forename: Jacques P
authors.initials: JP
authors.lastname: Stassart
authors.forename: G David
authors.initials: GD
authors.lastname: Ball
authors.forename: Ashley
authors.initials: A
authors.lastname: Wong
authors.forename: David H
authors.initials: DH
authors.lastname: Abbott
mesh_terms: D000735:Androstenedione / Q000032:analysis; D054304:Anti-Mullerian Hormone / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000032:analysis; D004624:Embryo Transfer / Q000379:methods*; D004958:Estradiol / Q000032:analysis; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000032:analysis / Q000627:therapeutic use*; D015571:Follicular Fluid / Q000737:chemistry; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D006801:Humans; D006080:Ovarian Follicle / Q000502:physiology*; D010060:Ovulation / Q000502:physiology*; D011247:Pregnancy; D011374:Progesterone / Q000032:analysis; D011994:Recombinant Proteins / Q000627:therapeutic use; D013739:Testosterone / Q000032:analysis
chemical_list: D011994:Recombinant Proteins; D013196:Dihydrotestosterone; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone