title: Image-guided Raman spectroscopic recovery of canine cortical bone contrast in situ.
abstract: Raman scattering provides valuable biochemical and molecular markers for studying bone tissue composition with use in predicting fracture risk in osteoporosis. Raman tomography can image through a few centimeters of tissue but is limited by low spatial resolution. X-ray computed tomography (CT) imaging can provide high-resolution image-guidance of the Raman spectroscopic characterization, which enhances the quantitative recovery of the Raman signals, and this technique provides additional information to standard imaging methods. This hypothesis was tested in data measured from Teflon tissue phantoms and from a canine limb. Image-guided Raman spectroscopy (IG-RS) of the canine limb using CT images of the tissue to guide the recovery recovered a contrast of 145:1 between the cortical bone and background. Considerably less contrast was found without the CT image to guide recovery. This study presents the first known IG-RS results from tissue and indicates that intrinsically high contrasts (on the order of a hundred fold) are available.
authors.affiliation: Dartmouth College, Thayer School of Engineering, Hanover, NH 03755, USA.
authors.forename: Subhadra
authors.initials: S
authors.lastname: Srinivasan
authors.forename: Matthew
authors.initials: M
authors.lastname: Schulmerich
authors.forename: Jacqueline H
authors.initials: JH
authors.lastname: Cole
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Dooley
authors.forename: Jaclynn M
authors.initials: JM
authors.lastname: Kreider
authors.forename: Brian W
authors.initials: BW
authors.lastname: Pogue
authors.forename: Michael D
authors.initials: MD
authors.lastname: Morris
authors.forename: Steven A
authors.initials: SA
authors.lastname: Goldstein
mesh_terms: D000818:Animals; D004285:Dogs; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D019047:Phantoms, Imaging; D011859:Radiography; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013977:Tibia / Q000737:chemistry* / Q000000981:diagnostic imaging