title: [Mapping of Rpn4p regions responsible for transcriptional activation of proteasome genes].
abstract: Rpn4p is positive and negative transcriptional regulator of the ubiquitin-proteasome system. At the same time, it is the extremely short-lived proteasome-associated protein and the proteasome substrate. Proteasome-dependent mechanisms of Rpn4p degradation is studied in details, but mechanisms of its action are not clear, and, first of all, functional domains is not defined. To map Rpn4p functionally important regions, we created a set of its deletion derivatives. Mutant proteins were expressed in rpn4-A yeast strain and their activities were estimated by measuring the mRNA level of proteasomal genes in appropriate transformants. We showed that Rpn4p devoid of its C-terminal region containing the zinc finger DNA-binding domains and its N-terminus, which shares no homology with transactivation domains of known transcription factors, was completely unable to activate transcription. Of two clusters of acid residues, proposed to be activation domains, only second one takes part in transcription. Moreover, deletion of N-terminus, proximal acidic domain or, surprisingly, zinc finger domains leads to Rpn4p stabilization. These data give new insight onto both mechanisms of Rpn4p action and degradation.
authors.forename: D S
authors.initials: DS
authors.lastname: Karpov
authors.forename: V V
authors.initials: VV
authors.lastname: Tiutiaeva
authors.forename: S F
authors.initials: SF
authors.lastname: Beresten'
authors.forename: V L
authors.initials: VL
authors.lastname: Karpov
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D010449:Peptide Mapping; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism; D016335:Zinc Fingers / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; C119430:RPN4 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex