Nitrate induction of root hydraulic conductivity in maize is not correlated with aquaporin expression.
Some plant species can increase the mass flow of water from the soil to the root surface in response to the appearance of nitrate in the rhizosphere by increasing root hydraulic conductivity. Such behavior can be seen as a powerful strategy to facilitate the uptake of nitrate in the patchy and dynamically changing soil environment. Despite the significance of such behavior, little is known about the dynamics and mechanism of this phenomenon. Here we examine root hydraulic response of nitrate starved Zea mays (L.) plants after a sudden exposure to 5 mM NO(3)(-) solution. In all cases the treatment resulted in a significant increase in pressure-induced (pressure gradient approximately 0.2 MPa) flow across the root system by approximately 50% within 4 h. Changes in osmotic gradient across the root were approximately 0.016 MPa (or 8.5%) and thus the results could only be explained by a true change in root hydraulic conductance. Anoxia treatment significantly reduced the effect of nitrate on xylem root hydraulic conductivity indicating an important role for aquaporins in this process. Despite a 1 h delay in the hydraulic response to nitrate treatment, we did not detect any change in the expression of six ZmPIP1 and seven ZmPIP2 genes, strongly suggesting that NO(3)(-) ions regulate root hydraulics at the protein level. Treatments with sodium tungstate (nitrate reductase inhibitor) aimed at resolving the information pathway regulating root hydraulic properties resulted in unexpected findings. Although this treatment blocked nitrate reductase activity and eliminated the nitrate-induced hydraulic response, it also produced changes in gene expression and nitrate uptake levels, precluding us from suggesting that nitrate acts on root hydraulic properties via the products of nitrate reductase.
Gorska
Anna
A
Arnold Arboretum, Harvard University, Cambridge, MA 02138, USA.
Zwieniecka
Anna
A
Holbrook
N Michele
NM
Zwieniecki
Maciej A
MA
D020346:Aquaporins / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D018527:Hydroponics; D009566:Nitrates / Q000494:pharmacology*; D018517:Plant Roots / Q000378:metabolism*; D014867:Water / Q000378:metabolism; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism*
D020346:Aquaporins; D009566:Nitrates; D014867:Water