In vitro and in vivo effect of lidocaine on rat muscle-derived cells for treatment of stress urinary incontinence.
OBJECTIVE
Lidocaine cytotoxicity has been reported in some cell types, which could affect its use as a local anesthetic in cell-based therapy. We evaluated the in vitro and in vivo effect of lidocaine on rat muscle-derived progenitor cells (MDCs).


METHODS
MDCs were isolated from rat skeletal muscle and purified using the preplate technique. For in vitro tests, the MDCs underwent either 2 hours of, or continuous, exposure to lidocaine (50 microM-5 mM). After 72 hours of incubation, cell viability was measured using the methylthiazololetetrazolium assay. For the in vivo tests, periurethral injection of either phosphate-buffered saline, MDCs (1 x 10(6) cells/20 microL), or 2% lidocaine plus MDCs was performed in pudendal nerve-transected rats. The leak point pressure (LPP) was measured at 4 weeks after the injection.


RESULTS
Lidocaine concentrations of < or = 500 microM had no effect on MDCs with continuous exposure. MDCs in 1 mM lidocaine showed decreased survival and no MDCs in 5 mM lidocaine survived. With a 2-hour exposure, only MDCs in the 5-mM lidocaine group showed decreased survival. Rats with nerve transection and phosphate-buffered saline injection showed significantly lower LPPs than the controls. The LPP was restored to a significantly greater level after MDCs only or lidocaine plus MDC injection. No statistically significant difference in LPP restoration was found between the MDC-only and lidocaine plus MDC injections.


CONCLUSIONS
Cytotoxicity to lidocaine was minimal at a physiologic concentration in vitro. The functional recovery of LPP by MDC treatment was not affected by lidocaine preinfiltration. Taken together, our data indicate that lidocaine can be applied as a local anesthetic in periurethral MDC injection without decreasing the efficacy of the therapy.
Kim
Dae Kyung
DK
Department of Urology, Eulji University, Daejeon, Republic of Korea.
Jankowski
Ron J
RJ
Pruchnic
Ryan
R
de Miguel
Fernando
F
Yoshimura
Naoki
N
Honda
Masashi
M
Furuta
Akira
A
Chancellor
Michael B
MB
D000779:Anesthetics, Local / Q000009:adverse effects / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D008012:Lidocaine / Q000009:adverse effects / Q000494:pharmacology*; D032342:Muscle Cells / Q000187:drug effects* / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014550:Urinary Incontinence, Stress / Q000601:surgery*
D000779:Anesthetics, Local; D008012:Lidocaine