title: Pea lectin in alkaline conditions: formation of molten globule-like intermediate and its structural and thermal studies under the influence of hexafluoroisopropanol.
abstract: Pea lectin was exposed to a pH range of 7-13. It was observed that alkaline-unfolding resulted in a molten globule-like intermediate at pH 11. The structural stability of this alkaline unfolded molten globule-like state of pea lectin was studied in the presence of HFIP. Thermal studies showed that this state was more susceptible to thermal denaturation as compared to the native state and it became even more so in presence of HFIP.
authors.affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh - 202002, India.
authors.forename: Farah
authors.initials: F
authors.lastname: Naseem
authors.forename: Rizwan Hasan
authors.initials: RH
authors.lastname: Khan
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry*; D020005:Propanols / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D013696:Temperature; D006845:Hydrocarbons, Fluorinated
chemical_list: D037121:Plant Lectins; D020005:Propanols; C036292:pea lectin; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated