Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri.
Peroxiredoxin V (PRX V) is known as an atypical 2-cysteine peroxiredoxin that protects the organisms against various oxidative stresses and functions in signal transduction. The cDNA of a PRX V gene (designated as CfPRX) was cloned from scallop Chlamys farreri. The full-length sequence of CfPRX cDNA was of 2,179 bp with a 564 bp open reading frame encoding a peptide of 187 amino acids. Sequence comparison showed that CfPRX shared higher identities with PRX Vs than that with other isoforms of PRX, indicating CfPRX was a member of the PRX V family. Fluorescent real-time quantitative PCR analysis revealed the presence of CfPRX transcripts in gill filaments, adductor muscle, heart, gonad, kidney and hemocytes, and the stimulation of Listonella anguillarum significantly (P < 0.01) enhanced the mRNA expression of CfPRX in hemocyte. These results indicated that CfPRX was a constitutive and inducible acute-phase protein which was involved in the immune resistance to L. anguillarum stimulation.
Cong
Ming
M
Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd, Qingdao, 266071, Shandong, China.
Ni
Duojiao
D
Song
Linsheng
L
Wang
Lingling
L
Zhao
Jianmin
J
Qiu
Limei
L
Li
Ling
L
D000209:Acute-Phase Proteins; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006434:Hemocytes; D007109:Immunity / Q000235:genetics; D049897:Pectinidae / Q000235:genetics* / Q000276:immunology; D054464:Peroxiredoxins / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger; D014018:Tissue Distribution
D000209:Acute-Phase Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D054464:Peroxiredoxins