title: Transposon Tn5.
abstract: Tn5 was one of the first transposons to be identified ( 10 ). As a result of Tn5's early discovery and its simple macromolecular requirements for transposition, the Tn5 system has been a very productive tool for studying the molecular mechanism of DNA transposition. These studies are of broad value because they offer insights into DNA transposition in general, because DNA transposition is a useful model with which to understand other types of protein-DNA interactions such as retroviral DNA integration and the DNA cleavage events involved in immunoglobulin gene formation, and because Tn5-derived tools are useful adjuncts in genetic experimentation.
authors.affiliation: Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Woods Hole, Massachusetts 02543, USA. breznikoff@mbl.edu
authors.forename: William S
authors.initials: WS
authors.lastname: Reznikoff
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D019427:HIV Integrase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D008958:Models, Molecular; D008967:Molecular Biology; D019895:Transposases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D019427:HIV Integrase; D019895:Transposases; C514801:p31 integrase protein, Human immunodeficiency virus 1