Inhibition of hepatitis B virus replication by small interference RNA induces expression of MICA in HepG2.2.15 cells.
Hepatitis B virus (HBV) replicates in most tumor tissues of patients with HBV-associated hepatocellular carcinoma (HCC). In the present study, we have shown that the expression of HBV in the HCC cell lines, HepG2 and Huh7, down-regulated the expression of MHC class I-related molecule A (MICA), a ligand of the NKG2D receptor. Inhibition of HBV expression by small interference RNAs (siRNAs) in HepG2.2.15, a cell line that constitutively expresses HBV, induced up-regulation of MICA. The up-regulation of MICA increased the lysis of HepG2.2.15 cells by NK cells. Our results suggest that HBV compromises the innate immune system in HCC patients and that inhibition of HBV replication by siRNAs may enhance the antitumor immune response.
Tang
Kai-Fu
KF
Chongqing University of Medical Sciences, Chongqing, 400010, People's Republic of China. tangkaifu@hotmail.com
Chen
Min
M
Xie
Jing
J
Song
Guan-Bin
GB
Shi
Yi-Song
YS
Liu
Qi
Q
Mei
Zhe-Chuan
ZC
Steinle
Alexander
A
Ren
Hong
H
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D005786:Gene Expression Regulation; D006515:Hepatitis B virus / Q000187:drug effects* / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D034741:RNA, Small Interfering