title: Effects of sodium azide, barium ion, d-amphetamine and procaine on inward rectifying potassium channel 6.2 expressed in Xenopus oocytes.
abstract: OBJECTIVE
  Inward rectifying potassium channel 6.2 (Kir6.2DelataC26 channel) is closely related to ATP-sensitive potassium channels. Whether sodium azide, barium ion, d-amphetamine or procaine acts directly on the Kir6.2DeltaC26 channel remains unclear. We studied the effects of these compounds on Kir6.2DeltaC26 channel expressed in Xenopus oocytes.
  
  
  METHODS
  The coding sequence of a truncated form of mouse Kir6.2 (GenBank accession number NP_034732.1), Kir6.2(1-364) (i.e. Kir6.2DeltaC26), was subcloned into the pET20b(+) vector. Plasmid containing the correct T7 promoter-Kir6.2(1-364) cDNA fragment [Kir6.2/pET20b(+)] was then subject to NotI digestion to generate the templates for in vitro run-off transcriptions. The channel was expressed in Xenopus laevis oocytes. Two-electrode voltage clamping was used to measure the effects of sodium azide, barium ion, d-amphetamine and procaine on Kir6.2DeltaC26 channel current.
  
  
  RESULTS
  Sodium azide activated and barium ion and d-amphetamine inhibited the Kir6.2DeltaC26 channel. Procaine did not have any significant effect on the Kir6.2DeltaC26 channel.
  
  
  CONCLUSIONS
  Kir6.2DeltaC26 channel expressed in Xenopus oocytes can be used as a pharmacological tool for the study of inward rectifying potassium channels.
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Fan-Lu
authors.initials: FL
authors.lastname: Kung
authors.forename: Jung-Lung
authors.initials: JL
authors.lastname: Tsai
authors.forename: Chien-Hsing
authors.initials: CH
authors.lastname: Lee
authors.forename: Kuo-Long
authors.initials: KL
authors.lastname: Lou
authors.forename: Chih-Yung
authors.initials: CY
authors.lastname: Tang
authors.forename: Horng-Huei
authors.initials: HH
authors.lastname: Liou
authors.forename: Kuan-Ling
authors.initials: KL
authors.lastname: Lu
authors.forename: Yi-Hung
authors.initials: YH
authors.lastname: Chen
authors.forename: Wun-Jheng
authors.initials: WJ
authors.lastname: Wang
authors.forename: Ming-Cheng
authors.initials: MC
authors.lastname: Tsai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001464:Barium / Q000494:pharmacology*; D001483:Base Sequence; D003913:Dextroamphetamine / Q000494:pharmacology*; D054086:KATP Channels / Q000187:drug effects; D008969:Molecular Sequence Data; D024661:Potassium Channels, Inwardly Rectifying / Q000187:drug effects*; D011343:Procaine / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000187:drug effects; D019810:Sodium Azide / Q000494:pharmacology*; D014982:Xenopus laevis; D000099161:Kcnj11 Channel
chemical_list: D001464:Barium; D003913:Dextroamphetamine; D054086:KATP Channels; D024661:Potassium Channels, Inwardly Rectifying; D011343:Procaine; D011994:Recombinant Proteins; D019810:Sodium Azide; D000099161:Kcnj11 Channel