Lack of association of Clara cell 10-kDa protein gene variant with chronic rhinosinusitis in a Chinese Han population.
BACKGROUND
Clara cell 10-kDa protein (CC10) is an anti-inflammatory molecule and has been implicated in the involvement of the pathogenesis of asthma and chronic rhinosinusitis (CRS). A single nucleotide polymorphism (SNP) in CC10 gene (A + 38G) was previously shown to be associated with asthma and plasma CC10 levels. The purpose of this study is to examine whether there is an association between the CC10 A + 38G SNP, plasma CC10 levels, and CRS in a central Chinese population of Han nationality.


METHODS
The CC10 A + 38G SNP was analyzed by means of polymerase chain reaction with restriction fragment length polymorphism and plasma CC10 levels were measured using enzyme-linked immunosorbent assay in 220 patients with CRS (90 patients with nasal polyps [NPs] and 130 patients without NPs) and 180 healthy control subjects. Among 220 patients with CRS, 108 patients were atopic subjects. Severity of disease was determined by coronal computed tomography (CT) scan in CRS patients, which was graded according to Lund and Mackay.


RESULTS
The frequency of the A allele was 0.394, which was not significantly higher than the frequencies of other reported ethnic groups except for German. No association between the CC10 A + 38G SNP and CRS, any subgroup of CRS, or CRS severity could be found. Although subjects carrying the AA genotype had a significantly lower plasma CC10 concentration than those carrying the GG and GA genotypes in both CRS and control groups (p = 0.00 for all), no association was found between the plasma CC10 levels and CRS phenotype.


CONCLUSIONS
The CC10 A + 38G SNP may not exert a substantial influence on the development of CRS in the Chinese Han population.
Zhang
Feng
F
Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University Of Science and Technology, Wuhan, China.
Xiong
Zhi-Gang
ZG
Cao
Ping-Ping
PP
You
Xue-Jun
XJ
Gao
Qi-Xue
QX
Cui
Yong-Hua
YH
Liu
Zheng
Z
D000328:Adult; D002681:China / Q000453:epidemiology; D002908:Chronic Disease; D004247:DNA / Q000235:genetics*; D004791:Enzyme Inhibitors; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D015995:Prevalence; D012220:Rhinitis / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D012852:Sinusitis / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D014598:Uteroglobin / Q000235:genetics* / Q000378:metabolism
D004791:Enzyme Inhibitors; C556389:SCGB1A1 protein, human; D004247:DNA; D014598:Uteroglobin