Inhibitory effects of vitamin K3 on DNA polymerase and angiogenesis.
Vitamins play essential roles in cellular reactions and maintain human health. Recent studies have revealed that some vitamins including D3, B6 and K2 and their derivatives have an anti-cancer effect. As a mechanism, their inhibitory effect on cancer-related angiogenesis has been demonstrated. Vitamin K2 (menaquinones) has an anti-cancer effect in particular for hepatic cancer and inhibits angiogenesis. In the current study, we demonstrated that sole vitamin K3 (menadione) selectively inhibits the in vitro activity of eukaryotic DNA polymerase gamma, which is a mitochondrial DNA polymerase, and suppresses angiogenesis in a rat aortic ring model. The anti-angiogenic effect of vitamin K3 has been shown in angiogenesis models using human umbilical vein endothelial cells (HUVECs) with regard to HUVEC growth, tube formation on reconstituted basement membrane and chemotaxis. These results suggest that vitamin K3 may be a potential anti-cancer agent like vitamin K2.
Matsubara
Kiminori
K
Department of Human Life Sciences Education, Graduate School of Education, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8524, Japan. kmatsuba@hiroshima-u.ac.jp
Kayashima
Tomoko
T
Mori
Masaharu
M
Yoshida
Hiromi
H
Mizushina
Yoshiyuki
Y
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017208:Rats, Wistar; D024483:Vitamin K 3 / Q000494:pharmacology*
D020533:Angiogenesis Inhibitors; D004791:Enzyme Inhibitors; D024483:Vitamin K 3; D004256:DNA Polymerase I