Effects of RANKL, osteoprotegerin, calcium and phosphorus on survival and activation of Muscovy duck osteoclasts in vitro.
The aim of this study was to determine whether receptor activator of nuclear factor NF-kappaB ligand (RANKL), osteoprotegerin (OPG) and a calcium:phosphorus (Ca:P) ratio of 2:1 could affect survival and activation of Muscovy duck osteoclasts (OCs). Bone marrow cells were obtained from 5-day-old Muscovy ducks and cultured with (Group A) No added factors, (B) 30ng/mL soluble RANKL (sRANKL), (C) 30ng/mL sRANKL and 10ng/mL OPG, (D) 10ng/mL OPG, (E) 50ng/mL OPG, (F) 100ng/mL OPG and (G) 30ng/mL sRANKL, 6mmol/L Ca and 3mmol/L P. sRANKL promoted the survival of OCs on day 2, whereas the number of OCs decreased with addition of OPG in a dose-dependent manner. OPG and Ca:P (2:1) both inhibited OC survival induced by RANKL. RANKL stimulated bone resorption by OCs, whereas OPG, but not Ca:P (2:1), inhibited the activity of OCs induced by RANKL. RANKL promotes the survival and activation of OCs from Muscovy ducks, whereas OPG and, to a lesser extent, Ca:P (2:1) reduce the life span and inhibited the activation of OCs induced by RANKL.
Gu
Jian-Hong
JH
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China.
Liu
Jun-Dong
JD
Shen
Yan
Y
Liu
Zong-Ping
ZP
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D004372:Ducks; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects*; D053244:Osteoprotegerin / Q000494:pharmacology*; D010758:Phosphorus / Q000494:pharmacology*; D053245:RANK Ligand / Q000494:pharmacology*
D053244:Osteoprotegerin; D053245:RANK Ligand; D010758:Phosphorus; D002118:Calcium