Oxidative DNA damage in bone marrow cells of patients with low-risk myelodysplastic syndrome.
Bone marrow aspirates of 19 patients with low-risk myelodysplastic syndromes (MDS) and 14 control subjects were collected in order to assess the level of oxidative DNA damage. Glycophorin A positive and negative cells separated by miniMACS magnetic cell sorting were subjected to single cell gel electrophoresis (comet assay) combined with enzymes of base excision repair (endonuclease III and formamido-pyrimidine-glycosylase) that specifically recognize oxidized nucleotides. Compared to controls, MDS patients exhibited a significant increase of oxidative damage to DNA which could contribute to genomic instability and disease progression.
Novotna
Bozena
B
Department of Genetic Ecotoxicology, Institute of Experimental Medicine, v.v.i., Academy of Sciences of the Czech Republic, Prague 142 20, Czech Republic. novotna@biomed.cas.cz
Bagryantseva
Yana
Y
Siskova
Magda
M
Neuwirtova
Radana
R
D000368:Aged; D000369:Aged, 80 and over; D000753:Anemia, Refractory; D000754:Anemia, Refractory, with Excess of Blasts; D001854:Bone Marrow Cells / Q000473:pathology*; D016022:Case-Control Studies; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D018384:Oxidative Stress