A PCR-based epidemiological survey of Hepatozoon canis in dogs in Nigeria.
The prevalence of Hepatozoon canis infections in dogs in Nigeria was surveyed using molecular methods. DNA was extracted from blood samples obtained from 400 dogs. A primer set that amplified the Babesia canis 18S rRNA gene, which has high similarity to the H. canis 18S rRNA gene, was used for the PCR. As a result, samples from 81 dogs (20.3%) produced 757 bp bands, which differed from the 698 bp band that corresponded to B. canis infection. The sequence of the PCR products of 10 samples were determined, all of which corresponded with the H. canis sequence.
Sasaki
Mizuki
M
Department of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.
Omobowale
Olutayo
O
Ohta
Kaisaku
K
Tozuka
Morito
M
Matsuu
Aya
A
Hirata
Haruyuki
H
Nottidge
Helen Oyebukola
HO
Ikadai
Hiromi
H
Oyamada
Takashi
T
D000818:Animals; D003044:Coccidia / Q000235:genetics / Q000302:isolation & purification*; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology*; D004285:Dogs; D005260:Female; D008297:Male; D009549:Nigeria / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics
D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S