Calumenin but not reticulocalbin forms a Ca2+-dependent complex with thrombospondin-1. A potential role in haemostasis and thrombosis.
Thrombocytes express thrombospondin-1 (TSP1), as well as the CREC proteins, calumenin and reticulocalbin. TSP1 and calumenin are released upon stimulation with thrombin. Calumenin has recently been shown to influence the synthesis of certain coagulation factors. Calumenin is present in atherosclerotic lesions but not in normal vasculature [Coppinger et al. (Blood 103:2096-2104, 2004)] and is able to modulate the protein expression pattern as well as the cell cycle of fibroblasts [Østergaard et al. (Proteomics 6:3509-3519, 2006)]. We here show that calumenin in the presence of Ca(2+) binds to TSP1 with a dissociation constant K (d) around 0.4 muM. This interaction is specific with respect to the secreted calumenin as the closest relative among the CREC family members, the non-secreted reticulocalbin, does not form a similar complex. This further indicates that calumenin may be broadly involved in haemostasis and in the pathophysiology of thrombosis.
Hansen
Gry Aune Westergaard
GA
Institute of Medical Biochemistry, University of Aarhus, Ole Worms Allé, Building 1170, 8000 Aarhus C, Denmark.
Vorum
Henrik
H
Jacobsen
Christian
C
Honoré
Bent
B
D000595:Amino Acid Sequence; D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006487:Hemostasis / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020349:Surface Plasmon Resonance; D013927:Thrombosis / Q000378:metabolism*; D019700:Thrombospondin 1 / Q000235:genetics / Q000378:metabolism*
C510527:CALU protein, human; D002135:Calcium-Binding Proteins; D010455:Peptides; D019700:Thrombospondin 1; C078865:reticulocalbin; D002118:Calcium