Monitoring of morphology and physical properties of cultured cells using a micro camera and a quartz crystal with transparent indium tin oxide electrodes after injections of glutaraldehyde and trypsin.
For investigating the effects of chemical stimulation to cultured cells, we have developed a quartz crystal sensor system with a micro charge-coupled device (CCD) camera that enables microphotograph imaging simultaneously with quartz crystal measurement. Human hepatoma cell line (HepG2) cells were cultured on the quartz crystal through a collagen film. The electrode of the quartz crystal was made of indium tin oxide (ITO) transparent electrodes that enable to obtain a transparent mode photograph. Glutaraldehyde and trypsin were injected to the chamber of the cells, respectively. The response of the quartz crystal was monitored and microphotographs were recorded, and the resonance frequency and resonance resistance were analyzed with an F-R diagram that plotted the resonance frequency and resonance resistance. In the case of the glutaraldehyde injection, the cells responded in two steps that included the fast response of the cross-linking reaction and the successive internal change in the cells. In the case of the trypsin injection, the responses included two processes. In the first step, cell adhesion factors were cleaved and the cell structure became round, and in the next step, the cells were deposited on the quartz crystal surface and the surface of the cells was directly in contact with the quartz crystal surface.
Kang
Hyen-Wook
HW
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachoji, Tokyo 192-0982, Japan.
Ida
Kazumi
K
Yamamoto
Yuji
Y
Muramatsu
Hiroshi
H
D015374:Biosensing Techniques / Q000379:methods*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D004492:Edetic Acid / Q000494:pharmacology; D005109:Extracellular Matrix / Q000737:chemistry; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D008839:Microelectrodes; D010781:Photography / Q000295:instrumentation; D011791:Quartz / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry*; D014357:Trypsin / Q000494:pharmacology
D017971:Tin Compounds; D011791:Quartz; C109984:indium tin oxide; D004492:Edetic Acid; D014357:Trypsin; D005976:Glutaral