title: Effect of a seashell protein Haishengsu on cell growth and expression of apoptosis genes in leukemia K562 cells.
abstract: OBJECTIVE
  To investigate the effect of a seashell protein Haishengsu (HSS), an extract from a shellfish Tegillarca granosa, on cell growth and the expression of apoptosis genes in leukemia K562 cells.
  
  
  METHODS
  Cultured K562 cells were treated with HSS at various concentrations (10-40 mg/L). The cell cycle, cell growth and the expression of apoptosis suppressor gene bcl-2 and apoptosis promoting gene bax were evaluated.
  
  
  RESULTS
  HSS, 20mg/L, inhibited cell cycle in the G0/G1 and S phases. HSS, 20mg/L, also inhibited the growth of K562 cells over time. Expression of bcl-2 gene in the HSS 20mg/L (58.8%+/-4.7%) and HSS 40 mg/L group (26.6%+/-2.1%) were lower than in the control group (91.0+/-8.7%, P < 0.01). Expression of bax gene in the HSS 20mg/L (77.7+/-3.6%) and 40 mg/L group (90.6+/-3.7%) were higher than in the control group (10.9+/-6.6%, P < 0.01).
  
  
  CONCLUSIONS
  HSS suppresses leukemia K562 cell growth by inhibiting the G0/G1 and S phases of the cell cycle. It also induces apoptosis in these leukemia cells by reducing the expression of apoptosis suppressor gene bcl-2, and increasing the expression of apoptosis promoting gene bax. Further studies are required to investigate the clinical efficacy of HSS in leukemia.
authors.affiliation: Liaocheng People's Hospital (Liaocheng Clinical School of Taishan Medical University), Liaocheng, Shandong, 252000, PR China.
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Li
authors.forename: Ji-Zhu
authors.initials: JZ
authors.lastname: Liu
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Yu
authors.forename: Shuang-Feng
authors.initials: SF
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Zhang
authors.forename: Tai-Wu
authors.initials: TW
authors.lastname: Xiao
authors.forename: Chun-Bo
authors.initials: CB
authors.lastname: Wang
authors.forename: Le-Xin
authors.initials: LX
authors.lastname: Wang
mesh_terms: D000418:Albumins / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D049893:Arcidae / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000502:physiology; D019254:Genes, bcl-2 / Q000235:genetics / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D020014:K562 Cells; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000418:Albumins; D004365:Drugs, Chinese Herbal; D051028:bcl-2-Associated X Protein; C532636:haishengsu