NHS-ester functionalized poly(PEGMA) brushes on silicon surface for covalent protein immobilization.
Poly(PEGMA) homopolymer brushes were developed by atom transfer radical polymerization (ATRP) on the initiator-modified silicon surface (Si-initiator). Through covalent binding, protein immobilization on the poly(PEGMA) films was enabled by further NHS-ester functionalization of the poly(PEGMA) chain ends. The formation of polymer brushes was confirmed by assessing the surface composition (XPS) and morphology (atomic force microscopy (AFM), scanning electronic microscopy (SEM)) of the modified silicon wafer. The binding performance of the NHS-ester functionalized surfaces with two proteins horseradish peroxidase (HRP) and chicken immunoglobulin (IgG) was monitored by direct observation. These results suggest that this method which incorporates the properties of polymer brush onto the binding surfaces may be a good strategy suitable for covalent protein immobilization.
Yao
Yang
Y
The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, PR China.
Ma
Yong-Zheng
YZ
Qin
Ming
M
Ma
Xiao-Jing
XJ
Wang
Chen
C
Feng
Xi-Zeng
XZ
D000586:Amination; D000818:Animals; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D002645:Chickens; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D007136:Immunoglobulins / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry*; D046208:Microchip Analytical Procedures; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*; D013053:Spectrophotometry; D013237:Stereoisomerism; D013388:Succinimides / Q000737:chemistry*; D013499:Surface Properties; D014965:X-Rays
D004800:Enzymes, Immobilized; D007136:Immunoglobulins; D008689:Methacrylates; D013388:Succinimides; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D006735:Horseradish Peroxidase; D012825:Silicon