Using enzymatic amplification by aldolase for the optical detection of DNA by an artificial signal cascade.
A two-step reaction cascade is applied to the sequence-specific detection of single-stranded DNA, including analyte-triggered re-activation of apo-aldolase by its cofactor Zn(2+) and catalytic conversion of a chromophore.
Graf
Nora
N
Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, 69120 Heidelberg, Germany.
Kassube
Susanne
S
Krämer
Roland
R
D002384:Catalysis; D002413:Cations, Divalent; D004271:DNA, Fungal / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004789:Enzyme Activation; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry; D055095:Optics and Photonics; D011725:Pyridines / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D015398:Signal Transduction; D015032:Zinc
D002413:Cations, Divalent; D004271:DNA, Fungal; D004277:DNA, Single-Stranded; D011725:Pyridines; D005634:Fructose-Bisphosphate Aldolase; D015032:Zinc; C023666:pyridine