title: Role of SraP in adherence of Staphylococcus aureus to the bovine mammary epithelia.
abstract: SraP, a platelet-binding surface protein of Staphylococcus aureus, is involved in the pathogenesis of infective endocarditis. In this study, we investigated the importance of SraP in the pathogenesis of bovine mastitis. By means of PCR, sraP was detected in all the isolates tested from bovine bulk milk and humans. However, SraP was not expressed on the cell surface in half of the bovine isolates. Moreover, disruption of sraP did not affect the ability of S. aureus to adhere to cultured bovine mammary epithelial cells. These results suggest that SraP does not seem to be an important factor for S. aureus to adhere to the bovine mammary epithelia.
authors.affiliation: Research Team for Bacterial/Parasitic Diseases, National Institute of Animal Health, 3-12-5 Kannondai, Tsukuba, Ibaraki, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takamatsu
authors.forename: Eiji
authors.initials: E
authors.lastname: Hata
authors.forename: Makoto
authors.initials: M
authors.lastname: Osaki
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
authors.forename: Sota
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sekizaki
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000502:physiology*; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002417:Cattle; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D006801:Humans; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D018829:Adhesins, Bacterial; D004269:DNA, Bacterial