title: [Effect of regulating intracellular ROS with antioxidants on the ex vivo expansion of cord blood CD34+ cells].
abstract: OBJECTIVE
  To investigate the effect of regulating intracellular ROS with antioxidants on the ex vivo expansion of cord blood CD34(+) cells.
  
  
  METHODS
  The levels of reactive oxygen species (ROS) in cord blood CD34(+) cells were reduced by superoxide dismutase (SOD), catalase (CAT) or N-acetylcysteine (NAC) in ex vivo expansion. The expansion of CD34(+) cells reducing ROS levels with antioxidant was studied.
  
  
  RESULTS
  It was observed that the generation of ROS was increased markedly by the cytokine combination. ROS was eliminated by antioxidant effectively. With the addition of antioxidant at different concentration, ROS was reduced to different levels. The percentage of CD34(+) cells and CD34(+)CD38(-) cells, the colony growth of colony-forming cells (CFC) and the re-expansion capability of CD34(+) cells were enhanced by 2 000 U/mL SOD, 200 U/mL CAT or 2 mmol/L NAC. However, its effect on fold expansion of CD34(+) cells was not significant. The expansion of the cells was inhibited by 8 000 U/mL SOD, 1 000 U/mL CAT or 5 mmol/L NAC.
  
  
  CONCLUSIONS
  The proportion of hematopoietic stem and progenitor cells in the culture substance was increased markedly with the addition of low dose antioxidants in ex vivo expansion.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. fjinli@hotmail.com
authors.forename: Jin-Li
authors.initials: JL
authors.lastname: Fan
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Cai
authors.forename: Wen-Song
authors.initials: WS
authors.lastname: Tan
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D018952:Antigens, CD34 / Q000378:metabolism*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D018952:Antigens, CD34; D000975:Antioxidants; D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase; D000111:Acetylcysteine