title: [Biological transport of minocycline by human periodontal ligament fibroblasts].
abstract: OBJECTIVE
  To investigate biological transport of minocycline by human periodontal ligament fibroblasts (HPDLF). To verify the hypothesis of delivering medicine to periodontium and the whole body through the root canal.
  
  
  METHODS
  HPDLF and MC3T3-E1 cells were incubated in minocycline solutions. The intracellular antibiotics contents were measured by high performance liquid chromatography (HPLC) and the cell total protein was measured by Bradford protein assay.
  
  
  RESULTS
  HPLC was an accurate, sensitive method for measurement of the intracellular minocycline. The incubation time and cell property had significant effect on the intracellular minocycline contents (P< 0.01). The intracellular contents increased with extracellular concentration.
  
  
  CONCLUSIONS
  Minocycline can be transported by HPDLF. The transport is concentration-dependent, time-dependent and cell specific.
authors.affiliation: Dental Institute, General Hospital of PLA, Beijing 100853, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Hong-Chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: E
authors.forename: Bin
authors.initials: B
authors.lastname: Leng
mesh_terms: D000900:Anti-Bacterial Agents; D001692:Biological Transport; D002478:Cells, Cultured; D005347:Fibroblasts; D006801:Humans; D008911:Minocycline; D010513:Periodontal Ligament
chemical_list: D000900:Anti-Bacterial Agents; D008911:Minocycline