Effect of FeSO4 treatment on glucose metabolism in diabetic rats.
Iron, the prosthetic group of haemoglobin, was found to lower serum glucose levels of diabetic rats. Its regulative mechanism and effects on enzymatic activities of glucose metabolism are still unknown. In this study, the correlation between iron supply and enzymatic activities of glucose metabolism and respiratory chain were evaluated in liver and kidney tissues of alloxan induced-diabetic rats. After FeSO4 and metformin administration, serum samples were collected for serum glucose and fructosamine level measurements. Kidney and liver tissues were excised at the end of the study for assaying enzymatic activities of isocitrate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, NADH-dehydrogenase and cytochrome-c-oxidase. Results showed significantly decreased serum glucose and fructosamine levels in treatment groups and enhanced enzymatic activities of several proteins as compared with the diabetic control group. Therefore, these data suggested that FeSO4 administration could increase the supply of oxygen, enhance enzymatic activities of glucose metabolism and the respiratory chain, accelerate glucose metabolism and consequently decrease serum glucose levels.
Zhang
Zhicai
Z
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, Guizhou 550002, People's Republic of China. hangzhicai2001@yahoo.com.cn
Lian
Bin
B
Cui
Fengjie
F
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D002952:Citric Acid Cycle / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects; D005296:Ferrous Compounds / Q000494:pharmacology*; D019270:Fructosamine / Q000097:blood; D006019:Glycolysis / Q000187:drug effects; D008297:Male; D008687:Metformin / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D005296:Ferrous Compounds; C020748:ferrous sulfate; D019270:Fructosamine; D008687:Metformin