title: A novel hairless mouse model on an atopic dermatitis-prone genetic background generated by receptor-mediated transgenesis.
abstract: Current mouse models for atopic dermatitis (AD) have a serious drawback, being the existence of dense hair on the body. Thus, a hairless animal model on an AD-prone genetic background will be a powerful tool to investigate the basis of and therapy for this complex disease. We applied the Toxin Receptor-mediated Cell Knockout (TRECK) method to generate a hairless transgenic (Tg) mice on the NC/Nga background, an AD-prone inbred strain. A minigene with the mouse Keratin71 (Krt71) promoter and human diphtheria toxin receptor, which intrinsically functions as the heparin-binding EGF-like growth factor, was introduced into the pronucleus of NC/Nga oocytes. Unexpectedly NCN24, one NC/Nga Tg line, showed a dominant hairless phenotype without diphtheria toxin administration. Furthermore, the atopic dermatitis-like predisposition and IgE elevation was observed in both NCN24 and the NC/Nga wildtype strain. NCN24 mice, which we have newly developed, will be useful to assess drugs for AD therapy, being able to monitor skin inflammation without shaving.
authors.affiliation: Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 3-18-22, Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan.
authors.forename: Toyoyuki
authors.initials: T
authors.lastname: Takada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shitara
authors.forename: Kunie
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Erika
authors.initials: E
authors.lastname: Kojima
authors.forename: Rie
authors.initials: R
authors.lastname: Ishii
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kikkawa
authors.forename: Choji
authors.initials: C
authors.lastname: Taya
authors.forename: Hajime
authors.initials: H
authors.lastname: Karasuyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Kohno
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yonekawa
mesh_terms: D000818:Animals; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000276:immunology; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D051379:Mice; D008812:Mice, Hairless; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic
chemical_list: C587883:HBEGF protein, human; C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D007073:Immunoglobulin E