title: Pediococcus pentosaceus NB-17 for probiotic use.
abstract: The plant-derived Pediococcus pentosaceus NB-17 was isolated from Japanese traditional vegetable pickles. The production of cytokines from mouse spleen cells co-cultivated with heat-killed bacteria was investigated in vitro. The bacteria significantly induced secretion levels of interferon (IFN)-gamma and interleukin (IL)-12 p70, and suppressed IL-4 productions in ovalbumin (OVA) sensitized mouse spleen cells. Therefore, the bacteria could effectively stimulate immune activities and showed allergic inhibitory effects. Further study on acid tolerance was performed under simulated gastric conditions and NB-17 showed resistance to simulated gastric acidity at pH 3.0 and pH 2.5. Moreover, after oral administration of the intact cells to rats, bacterial colonies derived from feces were analyzed by random amplification polymorphic DNA (RAPD). It was confirmed that the administered strain NB-17 remained alive in feces. These results suggest the possibility to use the P. pentosaceus NB-17 as functional foods.
authors.affiliation: Nisseibio Co., Ltd, 1-13 Megumino Kita 3-chome, Eniwa, Hokkaido 061-1374, Japan. ben@nisseibio.co.jp
authors.forename: Benjamas
authors.initials: B
authors.lastname: Jonganurakkun
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Shan Hua
authors.initials: SH
authors.lastname: Xu
authors.forename: Yuya
authors.initials: Y
authors.lastname: Tada
authors.forename: Kimiko
authors.initials: K
authors.lastname: Minamida
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yasokawa
authors.forename: Masahito
authors.initials: M
authors.lastname: Sugi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hara
authors.forename: Kozo
authors.initials: K
authors.lastname: Asano
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D005243:Feces / Q000382:microbiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010376:Pediococcus / Q000502:physiology*; D019936:Probiotics; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000276:immunology* / Q000382:microbiology*
chemical_list: D016207:Cytokines