Substrate evaluation for a microbeam endstation using unstained cell imaging.
A cellular imaging system, optimized for unstained cells seeded onto a thin substrate, is under development. This system will be a component of the endstation for the microbeam cell-irradiation facility at the University of Surrey. Previous irradiation experiments at the Gray Cancer Institute (GCI) have used Mylar film to support the cells [Folkard, M., Prise, K., Schettino, G., Shao, C., Gilchrist, S., Vojnovic, B., 2005. New insights into the cellular response to radiation using microbeams. Nucl. Instrum. Methods B 231, 189-194]. Although suitable for fluorescence microscopy, the Mylar often creates excessive optical noise when used with non-fluorescent microscopy. A variety of substrates are being investigated to provide appropriate optical clarity, cell adhesion, and radiation attenuation. This paper reports on our investigations to date.
Flaccavento
G
G
Gray Institute for Radiation Oncology & Biology, Radiobiology Research Institute, Churchill Hospital, Oxford OX3 7LJ, UK. giselle.flaccavento@rob.ox.ac.uk
Folkard
M
M
Noble
J A
JA
Prise
K M
KM
Vojnovic
B
B
D002448:Cell Adhesion; D002477:Cells / Q000528:radiation effects*; D003584:Cytological Techniques; D003952:Diagnostic Imaging / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D008853:Microscopy / Q000295:instrumentation*