[Clinical trials of Escherichia coli O-serogroup serodiagnosis in patients with diarrhea by Ec-LPS array].
After developing the Ec-LPS array for Escherichia coli O-serogroup serodiagnosis (J. Jpn. Infect. Dis., 2007; 81: 26-32), we tested the array's usefulness in sera bled over 8 years ago from 24 patients with pediatric diarrhea. IgM and IgA antibodies in 20 sera among sera from the 24 reacted with a single LPS spot, making it possible to diagnose the O-serogroup. IgG antibodies in almost all patient sera reacted with many LPS among the 58 O-serogroup LPS of E. coli. O-serogroup strains of E. coli isolated from the 24 patients numbered 15. Among 11 patients in who O-serogroups were serodiagnosed by both methods, 7 were diagnosed with the same O-serogroups. Based on these results, this array appears useful in the O-serogroup serodiagnosis of E. coli.
Amano
Ken-ichi
K
Bioscience Education Research Center, Akita University.
Tsutsumi
Reiko
R
Sato
Shigehiro
S
Tamura
Hiroshi
H
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification* / Q000276:immunology*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D007223:Infant; D019081:O Antigens / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*; D012703:Serotyping
D019081:O Antigens; D011135:Polysaccharides, Bacterial