title: Endometrial-peritoneal interactions during endometriotic lesion establishment.
abstract: The pathophysiology of endometriosis remains unclear but involves a complex interaction between ectopic endometrium and host peritoneal tissues. We hypothesized that disruption of this interaction would suppress endometriotic lesion formation. We hoped to delineate the molecular and cellular dialogue between ectopic human endometrium and peritoneal tissues in nude mice as a first step toward testing this hypothesis. Human endometrium was xenografted into nude mice, and the resulting lesions were analyzed using microarrays. A novel technique was developed that unambiguously determined whether RNA transcripts identified via microarray analyses originated from human cells (endometrium) or mouse cells (mesothelium). Four key pathways (ubiquitin/proteasome, inflammation, tissue remodeling/repair, and ras-mediated oncogenesis) were revealed, demonstrating communication between host mesothelial cells and ectopic endometrium. Morphometric analysis of nude mouse lesions confirmed that necrosis, inflammation, healing and repair, and cell proliferation occurred during xenograft development. These processes were entirely consistent with the molecular networks revealed by the microarray data. The transcripts detected in the xenografts overlapped with differentially expressed transcripts in a comparison between paired eutopic and ectopic endometria from human endometriotic patients. For the first time, components of the interaction between ectopic endometrium and peritoneal stromal tissues are revealed. Targeted disruption of this dialogue is likely to inhibit endometriotic tissue formation and may prove to be an effective therapeutic strategy for endometriosis.
authors.affiliation: Department of Pathology,, University of Cambridge, Cambridge, United Kingdom. louise.hull@adelaide.edu.au
authors.forename: M Louise
authors.initials: ML
authors.lastname: Hull
authors.forename: Claudia Rangel
authors.initials: CR
authors.lastname: Escareno
authors.forename: Jane M
authors.initials: JM
authors.lastname: Godsland
authors.forename: John R
authors.initials: JR
authors.lastname: Doig
authors.forename: Claire M
authors.initials: CM
authors.lastname: Johnson
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Phillips
authors.forename: Stephen K
authors.initials: SK
authors.lastname: Smith
authors.forename: Simon
authors.initials: S
authors.lastname: Tavaré
authors.forename: Cristin G
authors.initials: CG
authors.lastname: Print
authors.forename: D Stephen
authors.initials: DS
authors.lastname: Charnock-Jones
mesh_terms: D000328:Adult; D000818:Animals; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D004717:Endometrium / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010537:Peritoneum / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D014183:Transplantation, Heterologous