Modular arrangement of allelic variants explains the divergence in Moraxella catarrhalis UspA protein function.
Ubiquitous surface protein A molecules (UspAs) of Moraxella catarrhalis are large, nonfimbrial, autotransporter proteins that can be visualized as a "fuzzy" layer on the bacterial surface by transmission electron microscopy. Previous studies attributed a wide array of functions and binding activities to the closely related UspA1, UspA2, and/or UspA2H protein, yet the molecular and phylogenetic relationships among these activities remain largely unexplored. To address this issue, we determined the nucleotide sequence of the uspA1 genes from a variety of independent M. catarrhalis isolates and compared the deduced amino acid sequences to those of the previously characterized UspA1, UspA2, and UspA2H proteins. Rather than being conserved proteins, we observed a striking divergence of individual UspA1, UspA2, and UspA2H proteins resulting from the modular assortment of unrelated "cassettes" of peptide sequence. The exchange of certain variant cassettes correlates with strain-specific differences in UspA protein function and confers differing phenotypes upon these mucosal surface pathogens.
Brooks
Michael J
MJ
Department of Molecular Genetics, University of Toronto, Medical Sciences Building, Toronto, Ontario M5S 1A8, Canada.
Sedillo
Jennifer L
JL
Wagner
Nikki
N
Laurence
Cassie A
CA
Wang
Wei
W
Attia
Ahmed S
AS
Hansen
Eric J
EJ
Gray-Owen
Scott D
SD
D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D016384:Consensus Sequence / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D001936:Moraxella catarrhalis / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; C089675:UspA protein, Moraxella catarrhalis