Comparison of the membrane subproteomes during growth of a new pseudomonas strain on lysogeny broth medium, glucose, and phenol.
Study of the bacterial membrane proteome is a field of growing interest in the research of nutrient transport and processing. Pseudomonas sp. strain phDV1, a Gram-negative bacterium selected for its ability to degrade aromatic compounds, was monitored under different growth substrate conditions, using lysogeny broth medium (LB), glucose, and phenol as sole carbon source. The aim of this study was to characterize the membrane subproteomes of the Pseudomonas strain by proteomic means to assess the protein composition of this subcellular compartments, which appears fundamental for the biodegradation of aromatic compounds. A total number of 129 different proteins have been identified by MALDI-TOF/TOF, 19 of which are membrane proteins that belong to the inner membrane and 10 that belong to the outer membrane. Two membrane proteins were only expressed in the presence of the aromatic substrate. We identified a membrane protein involved in aromatic hydrocarbon degradation as well as a probable porin which may, in fact, function as an aromatic compound-specific porin. Although the presence of different transporters have been reported for different aromatic compounds such as toluene and benzoic acid, to our knowledge, these are the first phenol-inducible membrane transporters identified.
Papasotiriou
Dimitrios G
DG
Division of Biochemistry, Department of Chemistry, University of Crete, P.O. Box 2208, GR-71003 Voutes Heraklion, Greece.
Markoutsa
Stavroula
S
Meyer
Bjoern
B
Papadioti
Anastasia
A
Karas
Michael
M
Tsiotis
Georgios
G
D001426:Bacterial Proteins / Q000032:analysis* / Q000145:classification; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005947:Glucose / Q000378:metabolism; D007425:Intracellular Membranes / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis* / Q000145:classification; D008969:Molecular Sequence Data; D019800:Phenol / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D011549:Pseudomonas / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D003470:Culture Media; D008565:Membrane Proteins; D020543:Proteome; D019800:Phenol; D005947:Glucose