Anti-inflammatory activity of the extracts and fractions from Erigeron multiradiatus through bioassay-guided procedures.
BACKGROUND
Erigeron multiradiatus (Lindl.) Benth., an herb that grows in the alpine and subalpine meadow of Qinghai-Tibet plateau, has been widely used as a folk remedy by the native people for treatment of various inflammatory ailments.


OBJECTIVE
In order to isolate and identify the active components of Erigeron multiradiatus for anti-inflammatory activity, a preliminary phytochemical study and a bioassay-guided fractionation and purification process was performed.


METHODS
The dry whole plant Erigeron multiradiatus was extracted with 50% ethanol and then separated into CHCl(3), n-BuOH, and aqueous fractions. The anti-inflammatory activities of each fraction were investigated using two in vivo inflammation models.


RESULTS
These results exhibited varying degrees of anti-inflammatory activities and the n-BuOH fraction showed the strongest anti-inflammatory activities. The n-BuOH fraction was then subjected to separation and purification using macroporous resins column chromatography and Sephadex LH-20 leading to two flavonoids glucuronides identified as scutellarein-7-O-beta-glucuronide and apigenin-7-O-beta-glucuronide. Furthermore, LC-MS/MS identification and quantification of isolated compounds were also performed.


CONCLUSIONS
Scutellarein-7-O-beta-glucuronide and apigenin-7-O-beta-glucuronide were considered as major components and principally responsible for the anti-inflammatory activity of Erigeron multiradiatus. Thus the results of our study provide a scientific basis for the utilization of Erigeron multiradiatus in traditional Tibetan medicine.
Luo
Pei
P
West China School of Pharmacy, Sichuan University, No. 17, Section 3, Ren-Min-Nan-Lu Road, Chengdu, Sichuan 610041, China.
Zhang
Zhifeng
Z
Yi
Tao
T
Zhang
Hao
H
Liu
Xinfu
X
Mo
Zhengji
Z
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy; D049919:Erigeron / Q000737:chemistry*; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D005965:Glucuronates / Q000302:isolation & purification / Q000494:pharmacology; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D038861:Medicine, Tibetan Traditional; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018609:Tibet
D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D005965:Glucuronates; D010936:Plant Extracts; C456097:apigenin-7-O-glucuronide; C484876:scutellarin; D047310:Apigenin