[Influence of electromagnetic field on chosen parameters of thrombocytes' oxygen metabolism--in vitro research].
Being a natural environmental factor, an electromagnetic field exists from the beginning of the life on Earth and it has an influence on maintenance of life processes. Natural electromagnetic fields affect day and year rhythms of plants, animals and humans. As a result of an electromagnetic field's activity, there occurs a disorder of blood platelets' function, which may, in consequence, lead to acute and chronic conditions dangerous to health and life.


OBJECTIVE
The aim of this work was to assess the influence, which a shape of an electromagnetic field of low frequency has on generating free radicals and enzymatic activity of superoxide dismutase in human blood platelets.


METHODS
Suspension of human blood platelets was subjected to activity of electromagnetic field of different shapes, frequency of 50 Hz and induction of 10 mT for 15 and 30 minutes. An electromagnetic field was generated with Helmholtz coils arranged on a bracket, inside of which test tubes with the blood platelets' suspension were put. Next, they were subjected to an activity of a specific electromagnetic field.


RESULTS
The measurement of free radicals generation indicated an increase, in comparison with the initial values, after 15 minutes as well as 30 minutes of exposition, regardless of the electromagnetic field's shape, whereas the enzymatic activity of superoxide dismutase decreased, in comparison with the initial values, after 15 minutes as well as 30 minutes of exposition, regardless of the applied electromagnetic field's shape.


CONCLUSIONS
Basing on obtained results, it may be stated that the level of generating free radicals as well as the level of enzymatic activity of superoxide dismutase in tested blood cells indicates significant dependence on an electromagnetic field's shape. The greatest changes have been observed during the activity of a rectangular and triangular pulse.
Jankowski
Wojciech
W
Uniwersytet Medyczny w Łodzi, Zakład Edukacji Zdrowotnej i Promocji Zdrowia. promzdr@achilles.wam.lodz.pl
Henrykowska
Gabriela
G
Smigielski
Janusz
J
Pacholski
Krzysztof
K
Dziedziczak-Buczyńska
Maria
M
Kałka
Krzysztof
K
Buczyński
Andrzej
A
D001792:Blood Platelets / Q000378:metabolism* / Q000528:radiation effects*; D004574:Electromagnetic Fields; D005609:Free Radicals / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D010100:Oxygen / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
D005609:Free Radicals; D013482:Superoxide Dismutase; D010100:Oxygen