Neuronal differentiation modulates the dystrophin Dp71d binding to the nuclear matrix.
The function of dystrophin Dp71 in neuronal cells remains unknown. To approach this issue, we have selected the PC12 neuronal cell line. These cells express both a Dp71f cytoplasmic variant and a Dp71d nuclear isoform. In this study, we demonstrated by electron and confocal microscopy analyses of in situ nuclear matrices and Western blotting evaluation of cell extracts that Dp71d associates with the nuclear matrix. Interestingly, this binding is modulated during NGF-induced neuronal differentiation of PC12 cells with a twofold increment in the differentiated cells, compared to control cells. Also, distribution of Dp71d along the periphery of the nuclear matrix observed in the undifferentiated cells is replaced by intense fluorescent foci localized in the center of the nucleoskeletal structure. In summary, we revealed that Dp71d is a dynamic component of nuclear matrix that might participate in the nuclear modeling occurring during neuronal differentiation.
Rodríguez-Muñoz
Rafael
R
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Avenida Instituto Politécnico Nacional 2508, Apartado Postal 14-740, C.P. 07360, México D.F., Mexico.
Villarreal-Silva
Marcela
M
González-Ramírez
Ricardo
R
García-Sierra
Francisco
F
Mondragón
Mónica
M
Mondragón
Ricardo
R
Cerna
Joel
J
Cisneros
Bulmaro
B
D000818:Animals; D002454:Cell Differentiation; D016189:Dystrophin / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D015530:Nuclear Matrix / Q000378:metabolism*; D016716:PC12 Cells; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats
D016189:Dystrophin; D020033:Protein Isoforms; C085339:apo-dystrophin 1