title: Downregulation of muscle protein degradation in sepsis by eicosapentaenoic acid (EPA).
abstract: Eicosapentaenoic acid (EPA) has been shown to attenuate muscle atrophy in cancer, starvation and hyperthermia by downregulating the increased expression of the ubiquitin-proteasome proteolytic pathway leading to a reduction in protein degradation. In the current study EPA (0.5 g/kg) administered to septic mice completely attenuated the increased protein degradation in skeletal muscle by preventing the increase in both gene expression and protein concentration of the alpha- and beta-subunits of the 20S proteasome, as well as functional activity of the proteasome, as measured by the 'chymotrypsin-like' enzyme activity. These results suggest that muscle protein catabolism in sepsis is mediated by the same intracellular signalling pathways as found in other catabolic conditions.
authors.affiliation: Nutritional Biomedicine, School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK.
authors.forename: Jwan
authors.initials: J
authors.lastname: Khal
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Tisdale
mesh_terms: D000818:Animals; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D009133:Muscular Atrophy / Q000209:etiology / Q000378:metabolism / Q000382:microbiology; D046988:Proteasome Endopeptidase Complex / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis; D018805:Sepsis / Q000150:complications / Q000378:metabolism*; D013211:Staphylococcus aureus
chemical_list: D009124:Muscle Proteins; D012333:RNA, Messenger; D015118:Eicosapentaenoic Acid; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; C096184:proteasome component C5