A modified CZ-1 preserving solution for organ transplantation: comparative study with UW preserving solution.
BACKGROUND
The University of Wisconsin colloid based preserving solution (UW solution) is the most efficient preserving solution for multiorgan transplantation. Unfortunately, unavailability of delayed organ preserving solutions hindered further progression of cardinal organ transplantation in China. In this study, we validated an organ preserving Changzheng Organ Preserving Solution (CZ-1 solution) and compared it with UW solution.


METHODS
A series of studies were conducted on how and how long CZ-1 solution could preserve the kidneys, livers, hearts, lungs and pancreas of New Zealand rabbits and SD rats. Morphology of transplanted organs was studied by visible microscopy and electron microscopy; biochemical and physiological functions and the survival rate of the organs during prolonged cold storage were studied.


RESULTS
There was no significant difference between CZ-1 and UW solutions in preserving the kidneys, livers, hearts or lungs of rabbits; kidneys, livers, intestinal mucosa or pancreases of SD rats or five deceased donors' testicles. In some aspects, such as preserving rabbits' hearts, rats' intestinal mucosa and pancreases, the effect of CZ-1 solution was superior to UW solution. CZ-1 could safely preserve kidneys for 72 hours, livers for 24 hours, hearts for 18 hours and lungs for 8 hours for SD rats. Twelve kidneys preserved in cold CZ-1 solution for 22 - 31 hours were transplanted successfully and the mean renal function recovery time was (3.83 +/- 1.68) days.


CONCLUSIONS
CZ-1 solution is as effective as UW solution for organ preservation. The development of CZ-1 solution not only reduces costs and improves preservation of organs, but also promotes future development of organ transplantation in China.
Zheng
Jun-hua
JH
Department of Urology, Shanghai 10th People's Hospital, Tongji University, Shanghai 200072, China. zhengjh0471@sina.com
Min
Zhi-lian
ZL
Li
Yu-li
YL
Zhu
You-hua
YH
Ye
Ting-jun
TJ
Li
Jian-qiu
JQ
Pan
Tie-wen
TW
Ding
Guo-shan
GS
Wang
Meng-long
ML
D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D002681:China; D005978:Glutathione / Q000494:pharmacology; D006321:Heart / Q000187:drug effects / Q000502:physiology; D016027:Heart Transplantation / Q000379:methods; D007328:Insulin / Q000494:pharmacology; D007421:Intestine, Small / Q000187:drug effects / Q000502:physiology; D007668:Kidney / Q000187:drug effects / Q000502:physiology; D016030:Kidney Transplantation / Q000379:methods; D008099:Liver / Q000187:drug effects / Q000502:physiology; D016031:Liver Transplantation / Q000379:methods; D008168:Lung / Q000187:drug effects / Q000502:physiology; D016040:Lung Transplantation / Q000379:methods; D008297:Male; D009926:Organ Preservation / Q000191:economics / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects / Q000502:physiology; D016035:Pancreas Transplantation / Q000379:methods; D019999:Pharmaceutical Solutions / Q000494:pharmacology*; D011817:Rabbits; D011887:Raffinose / Q000494:pharmacology; D013737:Testis / Q000187:drug effects / Q000502:physiology
D007328:Insulin; D019188:Organ Preservation Solutions; D019999:Pharmaceutical Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose