title: Angiotensin II induces apoptosis in human mural granulosa-lutein cells, but not in cumulus cells.
abstract: OBJECTIVE
  To test whether angiotensin II (AngII) could modulate apoptosis of human granulosa-lutein (GL) cells from gonadotropin-stimulated follicles.
  
  
  METHODS
  In vitro assays on mural and cumulus granulosa cells.
  
  
  METHODS
  University laboratory and private IVF practice.
  
  
  METHODS
  One hundred six consecutive women undergoing 113 IVF cycles.
  
  
  METHODS
  Purified human GL mural or cumulus cells were cultured in serum-free media in the presence or absence of AngII with or without the AngII receptor blockers saralasin and CGP42112A.
  
  
  METHODS
  Detection of apoptosis using a fluorescent in situ marker for activated caspases.
  
  
  RESULTS
  Mural cells had approximately eightfold the amount of apoptosis compared with cumulus cells (average 0.23 vs. <0.03, respectively). With mural cells, AngII increased GL cell apoptosis versus untreated control samples (AngII 10(-)11 mol/L +6.5%; AngII 10(-9) mol/L +13.3%, and AngII 10(-7) mol/L +11.3%), an effect which was blocked by concurrent incubation with AngII receptor blockers. The AngII receptor blockers produced a significant decrease of apoptosis compared with control cultures (saralasin: 19.4%; CGP42112A: 28.9%). Neither AngII nor blockers had effect on cumulus cells.
  
  
  CONCLUSIONS
  Preovulatory concentrations of AngII, most likely via AT2 receptors, increase apoptosis of cultured mural GL cells but have no effect on cumulus cells. Granulosa cells appear to be differentially regulated by AngII.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of La Laguna, Tenerife, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Acosta
authors.forename: Oscar
authors.initials: O
authors.lastname: Peña
authors.forename: Frederick
authors.initials: F
authors.lastname: Naftolin
authors.forename: Julio
authors.initials: J
authors.lastname: Avila
authors.forename: Angela
authors.initials: A
authors.lastname: Palumbo
mesh_terms: D000293:Adolescent; D000328:Adult; D000375:Aging / Q000502:physiology; D000804:Angiotensin II / Q000494:pharmacology*; D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D018929:Cell Culture Techniques; D016895:Culture Media, Serum-Free; D054885:Cumulus Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000097:blood; D006107:Granulosa Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008597:Menstrual Cycle; D010062:Ovulation Induction; D018579:Patient Selection; D044140:Receptor, Angiotensin, Type 1 / Q000187:drug effects / Q000502:physiology; D055815:Young Adult
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D016895:Culture Media, Serum-Free; D044140:Receptor, Angiotensin, Type 1; D000804:Angiotensin II; D005640:Follicle Stimulating Hormone