[Promoter activity of B-cell maturation antigen gene in mice].
OBJECTIVE
To investigate the promoter activity of BCMA gene 5'-flanking regionfor the study the regulation mechanism of BCMA expression.


METHODS
Luciferase reporter plasmids containing 5'-flanking region of BCMA gene and serial deletions of the fragment were constructed. The luciferase expression was observed after these reporters were transfected into J558L, 293T and Hela cells.


RESULTS
The highest transcriptional activation was expressed in pGL3-B157 reporter, followed by pGL3-B607, pGL3-B359, pGL3-B93, and pGL3-B820 in sequence. The highest transcriptional activation was in J558L cell.


CONCLUSIONS
The 5'-flanking sequence from -820 to +145 bp of BCMA is of promoter activity. Serial deletion analysis of the promoter region of BCMA gene suggests the sequence from -93 to +145 could be a core promoter region.
Deng
Shao-Li
SL
Department of Medical Lab. Daping Hospital, Third Military Medical University, Chongqing 400038, China. dengl072@yahoo.com.cn
Cheng
Xiao-Xing
XX
Jian
Rui
R
Jiang
Jing
J
D000818:Animals; D053301:B-Cell Maturation Antigen / Q000235:genetics*; D002460:Cell Line; D006801:Humans; D051379:Mice; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics; D014162:Transfection
D053301:B-Cell Maturation Antigen