Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye.
A recombinant laccase from Trametes versicolor in Pichia methanolica was produced constitutively in a defined medium. The recombinant laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration. The molecular weight of the purified laccase was estimated as 64 kDa by SDS-PAGE. The purified recombinant laccase decolorized more than 90% of Remazol Brilliant Blue R (RBBR) initially at 80 mg l(-1) after 16 h at 45 degrees C and pH 5 when 25 U laccase ml(-1) was used. The purified recombinant laccase could efficiently decolorize RBBR without additional redox mediators.
Guo
Mei
M
Department of Food Science, Tianjin Agriculture College, Tianjin, 300384, People's Republic of China.
Lu
Fuping
F
Liu
Minyao
M
Li
Tuoping
T
Pu
Jun
J
Wang
Na
N
Liang
Peng
P
Zhang
Chenyun
C
D000880:Anthraquinones / Q000737:chemistry*; D001487:Basidiomycota / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004396:Coloring Agents / Q000737:chemistry*; D042845:Laccase / Q000302:isolation & purification* / Q000378:metabolism*; D010843:Pichia / Q000235:genetics*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014462:Ultrafiltration
D000880:Anthraquinones; D004396:Coloring Agents; D011994:Recombinant Proteins; D042845:Laccase; C024758:Remazol Brilliant Blue R