Complete genome of Phenylobacterium zucineum--a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562.
BACKGROUND
Phenylobacterium zucineum is a recently identified facultative intracellular species isolated from the human leukemia cell line K562. Unlike the known intracellular pathogens, P. zucineum maintains a stable association with its host cell without affecting the growth and morphology of the latter.


RESULTS
Here, we report the whole genome sequence of the type strain HLK1T. The genome consists of a circular chromosome (3,996,255 bp) and a circular plasmid (382,976 bp). It encodes 3,861 putative proteins, 42 tRNAs, and a 16S-23S-5S rRNA operon. Comparative genomic analysis revealed that it is phylogenetically closest to Caulobacter crescentus, a model species for cell cycle research. Notably, P. zucineum has a gene that is strikingly similar, both structurally and functionally, to the cell cycle master regulator CtrA of C. crescentus, and most of the genes directly regulated by CtrA in the latter have orthologs in the former.


CONCLUSIONS
This work presents the first complete bacterial genome in the genus Phenylobacterium. Comparative genomic analysis indicated that the CtrA regulon is well conserved between C. crescentus and P. zucineum.
Luo
Yingfeng
Y
Cancer Institute, (Key Laboratory for Cancer Intervention and Prevention, Key Laboratory of Molecular Biology in Medical Sciences), Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, PR China. luoyf@big.ac.cn
Xu
Xiaoli
X
Ding
Zonghui
Z
Liu
Zhen
Z
Zhang
Bing
B
Yan
Zhiyu
Z
Sun
Jie
J
Hu
Songnian
S
Hu
Xun
X
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D042063:Caulobacteraceae / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D015698:Genomic Library; D023281:Genomics; D006801:Humans; D020014:K562 Cells; D008969:Molecular Sequence Data; D009876:Operon; D010802:Phylogeny; D010957:Plasmids; D018085:Regulon; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; C097706:CtrA protein, Caulobacter; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D014157:Transcription Factors