A Western blot to detect antibody to avian reovirus.
Purified reoviruses RAM-1 and 74/2 were combined and used as antigen. The Western blot detected antibodies to 16/16 avian reoviruses in sera from experimentally inoculated chickens, and to 6/7 reoviruses in contact sera from naturally infected chickens. The test was specific for avian reovirus and was more sensitive and specific than immunofluorescence.
Endo-Munoz
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National Biological Standards Laboratory, Parkville, Victoria, Australia.