title: Effect of phospholipid insertion on arrayed polydiacetylene biosensors.
abstract: Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition.
authors.affiliation: Department of Chemical and Biological Engineering and Center for Integrated-Nano Systems, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, Republic of Korea.
authors.forename: Kyung-Woo
authors.initials: KW
authors.lastname: Kim
authors.forename: Hyun
authors.initials: H
authors.lastname: Choi
authors.forename: Gil Sun
authors.initials: GS
authors.lastname: Lee
authors.forename: Dong June
authors.initials: DJ
authors.lastname: Ahn
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Oh
mesh_terms: D000577:Amides / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002264:Carboxylic Acids / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry; D005453:Fluorescence; D005898:Glass / Q000737:chemistry; D006860:Hydrogen Bonding; D008958:Models, Molecular; D015394:Molecular Structure; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D040081:Protein Array Analysis; D012486:Salmonella typhimurium / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000577:Amides; D000906:Antibodies; D002264:Carboxylic Acids; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D004134:Dimyristoylphosphatidylcholine