A viral suppressor P1/HC-pro increases the GFP gene expression in agrobacterium-mediated transient assay.
More than 20 post-transcriptional gene silencing (PTGS) suppressors have been found since HC-Pro, the first gene silencing suppressor, was found in 1998. The silencing suppressor strongly suggested that gene silencing functions as natural defense mechanisms against viruses. It also represented a valuable tool for the dissection of the gene silencing pathway. We have used P1/HC-Pro RNA silencing suppressor activity to increase green fluorescent protein (GFP) expression in tobacco using an Agrobacterium-mediated transient expression system. P1/HC-Pro stimulated GFP-gene expression but not dsGFP-gene expression was shown by RT-PCR, Northern and Western blot analysis. Expression of the gene silencing suppressor and the target gene provided a new strategy of heterogeneous gene expressing in plants. It may be of commercial significance to produce foreign proteins using plant bioreactors.
Ma
Pengda
P
Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
Liu
Jinying
J
He
Hongxia
H
Yang
Meiying
M
Li
Meina
M
Zhu
Xiaojuan
X
Wang
Xingzhi
X
D015152:Blotting, Northern; D015153:Blotting, Western; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus