title: Targeted metabolite analysis and antioxidant potential of Rumex induratus.
abstract: Targeted metabolite analysis of aqueous extract of Rumex induratus leaves, in terms of phenolic compounds and organic acids, and the study of its antioxidant activity against the DPPH(*) radical, a reactive oxygen species, hypochlorous acid, and a reactive nitrogen species, nitric oxide ((*)NO), were performed. The samples were collected in several locations, spontaneously occurring or from greenhouse culture, at different stages of development and seasons. The phenolic composition was achieved by high-performance liquid chromatography (HPLC)-diode array detection, and four hydroxycinnamic acid derivatives and 10 flavonoid glycosides (C- and O-heterosides) were determined. Organic acids composition was established by HPLC-UV, revealing five compounds. The total amount of phenolic compounds and organic acids were affected by growing conditions and developmental phase. The aqueous extract exhibited a dose-related activity against all tested reactive species.
authors.affiliation: REQUIMTE/Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 164, 4050-047 Porto, Portugal.
authors.forename: Luís
authors.initials: L
authors.lastname: Guerra
authors.forename: Clara
authors.initials: C
authors.lastname: Pereira
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
authors.forename: M Angelo
authors.initials: MA
authors.lastname: Rodrigues
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Paula Guedes
authors.initials: PG
authors.lastname: De Pinho
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Seabra
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D010636:Phenols / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry*; D031804:Rumex / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002264:Carboxylic Acids; D003373:Coumaric Acids; D005419:Flavonoids; D010636:Phenols