Safety, immunogenicity, and efficacy of the ML29 reassortant vaccine for Lassa fever in small non-human primates.
A single injection of ML29 reassortant vaccine for Lassa fever induces low, transient viremia, and low or moderate levels of ML29 replication in tissues of common marmosets depending on the dose of the vaccination. The vaccination elicits specific immune responses and completely protects marmosets against fatal disease by induction of sterilizing cell-mediated immunity. DNA array analysis of human peripheral blood mononuclear cells from healthy donors exposed to ML29 revealed that gene expression patterns in ML29-exposed PBMC and control, media-exposed PBMC, clustered together confirming safety profile of the ML29 in non-human primates. The ML29 reassortant is a promising vaccine candidate for Lassa fever.
Lukashevich
Igor S
IS
Institute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, United States. ilukashevich@ihv.umaryland.edu
Carrion
Ricardo
R
Salvato
Maria S
MS
Mansfield
Keith
K
Brasky
Kathleen
K
Zapata
Juan
J
Cairo
Cristiana
C
Goicochea
Marco
M
Hoosien
Gia E
GE
Ticer
Anysha
A
Bryant
Joseph
J
Davis
Harry
H
Hammamieh
Rasha
R
Mayda
Maria
M
Jett
Marti
M
Patterson
Jean
J
D000818:Animals; D017252:CD3 Complex / Q000276:immunology; D002144:Callithrix / Q000276:immunology*; D002522:Chlorocebus aethiops; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D007835:Lassa Fever / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D007836:Lassa virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007963:Leukocytes, Mononuclear / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008297:Male; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology*; D012449:Safety; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology; D014709:Vero Cells; D014766:Viremia / Q000276:immunology
D017252:CD3 Complex; D018950:Lipopolysaccharide Receptors