[Influence of ASODN to the human tenon's fibroblasts in expressing CTGF induced by transforming growth factor beta2].
OBJECTIVE
To investigate the effect of connective tissue growth factor's antisense oligonucleotides (ASODN) on the growth of human tenon' s capsule fibroblasts (HTF) induced by transforming growth factor beta2 (TGF-beta2) in vitro.


METHODS
It was a experimental study. HTF was collected from glaucoma patients and cultured. The 5-6 passage was used for experiments. The HTF induced by TGF-beta2 was divided into the following groups: N group: normal HTF; T group: HTF induced by TGF-beta2; A group: CTGF ASODN antisense:5'-TACTGGCGGCGGTCAT-3' encapsulated with liposome; S group: sense 5'-ATGACCGCCGCCAGTA-3' encapsulated with liposome; D group: HTF encapsulated with liposome only. The activity of HTF treated by different concentrations of liposome was detected using methylthianolyldiphenyl tetrazolium bromide (MT) colorimetry. The expression of CTGF was detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry assays. The expression of fibronectin (Fn) was examined by Western blot and immunocytochemistry assays.


RESULTS
Liposome-ASODN (A group) significantly (F=15.25, 204.88, 19.73, 90.00; P <0.05) inhibit the expression of CTGF and Fn in HTF induced by TGF-beta2 compared with S and D group. However, Liposome alone (T group) has no significant impact in HTF growth compared with T group (t = 0.90, 2.32, 0.75, 2.11; P > 0.05).


CONCLUSIONS
CTGF-ASODN inhibits the CTGF and Fn expression of HTF induced by TGF-beta2, which may delay the formation of scar in glaucoma filtering surgery.
Hu
Yi-Zhen
YZ
Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Wang
Yu-Hong
YH
Cao
Yang
Y
Zhang
Ming-Chang
MC
D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor / Q000378:metabolism*; D005123:Eye / Q000166:cytology / Q000187:drug effects*; D005205:Fascia / Q000166:cytology / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D053781:Transforming Growth Factor beta2 / Q000494:pharmacology
D005353:Fibronectins; D016376:Oligonucleotides, Antisense; D053781:Transforming Growth Factor beta2; D055513:Connective Tissue Growth Factor