De novo shoot regeneration from root cultures of Garcinia indica Choiss.
Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 microM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented with BAP (4.44-22.69 microM), IAA (5.71 microM) and kinetin (4.65 microM). It was observed that shoot length increased to 1-2 cm. WPM medium supplemented with NAA (2.69-10.74 microM) and IBA (4.90 microM) induced rooting within 20-25 days. Using the present protocol, 20-25 plantlets could be regenerated from single root explant within 3 to 4 months. The protocol has potential for large scale production of elite plants.
Deodhar
Swapna R
SR
Plant Tissue Culture Division, National Chemical Laboratory, Pune 411008, India.
Thengane
R J
RJ
Thengane
S R
SR
D046508:Culture Techniques; D029760:Garcinia / Q000254:growth & development*; D018517:Plant Roots; D018520:Plant Shoots / Q000502:physiology*; D012038:Regeneration