[Experimental study of relationship of bile composition imbalance with bile duct injury].
OBJECTIVE
To investigate the change of bile composition and its role in bile duct injury after orthotopic liver transplantation (OLT).


METHODS
Rats were randomly divided into 3 groups: group A (sham surgery), group B (OLT with 1 h cold preservation), group C (OLT with 12 h cold preservation). The arterialized rat liver transplantation model with biliary extra-drainage was used in group B and C. Animals were sacrificed at posttransplant 1, 3, 5, 7, 10 and 14 day. Routine bile chemistry and pathological assays were performed.


RESULTS
Cold preservation/reperfusion injury (CPRI) could repress the secretion of bile salts and phospholipid. However, in contrast with a rapid increase of bile salt secretion, the biliary secretion of phospholipid recovered more slowly, leading to an abnormal high bile salts/phospholipid ratio early after transplantation. Further analysis suggested that the secretion of bile salts correlated strongly with biochemical and histopathological signs of bile duct injury.


CONCLUSIONS
CPRI can lead to great changes of graft bile composition, which plays a role in the pathogenesis of bile duct injury following liver transplantation.
Chen
Geng
G
Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Zhang
Yu-Jun
YJ
Yang
Cheng
C
Li
Kun
K
Li
Xiao-Wu
XW
Wang
Shu-Guang
SG
Dong
Jia-Hong
JH
D000818:Animals; D001646:Bile / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism; D001649:Bile Duct Diseases / Q000209:etiology*; D001653:Bile Ducts, Intrahepatic / Q000473:pathology; D050377:Cold Ischemia; D004195:Disease Models, Animal; D016031:Liver Transplantation; D008297:Male; D011183:Postoperative Complications / Q000209:etiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications / Q000378:metabolism / Q000473:pathology
D001647:Bile Acids and Salts