title: Phage display screening for peptidic chitinase inhibitors.
abstract: A phage display library with disulfide-cyclized peptides was screened for peptides binding to chitinases from Serratia marcescens. One of those peptides was found to efficiently inhibit chitinase A and two others were inhibitors of chitinase B. Complete substitutional analysis of all three peptides using cellulose-bound peptide spot synthesis revealed key interaction positions and allowed optimization of the chitinase B inhibitory peptides towards higher affinity, with inhibitory constants in the lower nanomolar range. Inhibition by all peptides proved to be competitive and highly specific for the chitinase used to select them, as shown with a series of chitinases from different organisms.
authors.affiliation: Institute of Biochemistry, Charité- Universitätsmedizin Berlin, Monbijoustrasse 2, D-10117 Berlin, Germany.
authors.forename: Cordula
authors.initials: C
authors.lastname: Petter
authors.forename: Christa
authors.initials: C
authors.lastname: Scholz
authors.forename: Helga
authors.initials: H
authors.lastname: Wessner
authors.forename: Gerd
authors.initials: G
authors.lastname: Hansen
authors.forename: Peter
authors.initials: P
authors.lastname: Henklein
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Höhne
mesh_terms: D002688:Chitinases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D019151:Peptide Library
chemical_list: D004791:Enzyme Inhibitors; D019151:Peptide Library; D002688:Chitinases