Use of a commercial protease and yeasts to obtain CGRP-like molecules from saithe protein.
Different bioactive molecules, such as CGRP-like peptides, can be found in fish protein hydrolysates. Calcitonin gene-related peptide (CGRP) is a neuropeptide known to act as a potent arterial and venous vasodilator in humans. This study focuses on the industrial obtaining of CGRP-like molecules from saithe (Pollachius virens) byproduct, derived from the filleting process. Protein from P. virens was primarily hydrolyzed with Alcalase and later treated with Saccharomyces cerevisiae live cells. Treatment with Saccharomyces doubled the quantity of bioactive molecules obtained. The CGRP-like molecules were partially purified by chromatography, and the immunoreactive material was further analyzed for its CGRP-like bioactivity, using a specific radioreceptor assay. The concentration of CGRP-like molecules increased over 100-fold after purification. The bioactive molecules were able to induce cyclic AMP stimulation in rat liver membranes. Finally, partial sequencing of the bioactive peptide was performed, showing some homology with alpha-actin and myosin of several fish species.
Martínez-Alvarez
Oscar
O
Muséum National d'Histoire Naturelle USM 405, UMR CNRS/MNHN/UPMC 5178, Station Biologie Marine, B.P. 225, 29182 Concarneau Cedex, France. oscar.martinez@if.csic.es
Guimas
Laurence
L
Delannoy
Charles
C
Fouchereau-Peron
Martine
M
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D029941:Fish Proteins / Q000737:chemistry* / Q000378:metabolism; D048388:Gadiformes; D010447:Peptide Hydrolases / Q000378:metabolism*; D011869:Radioligand Assay; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D013381:Subtilisins / Q000378:metabolism
D029941:Fish Proteins; D010447:Peptide Hydrolases; D013381:Subtilisins; D015740:Calcitonin Gene-Related Peptide