Identification of E1AF as a target gene of E2F1-induced apoptosis in response to DNA damage.
Transcription factor E1AF plays critical roles in neuronal development and tumour metastasis and is regulated by a number of signalling cascades, including the mitogen-activated protein kinase pathways. Accumulated evidence indicted that E1AF might contribute to cell survival in response to environment factors. Here, we provided evidence the cell cycle and apoptosis regulator E2F1 induces E1AF expression at the transcriptional level. DNA damage by etoposide causes E2F1-dependent induction of E1AF expression at transcriptional level. Furthermore, disruption of E1AF expression by E1AF RNAi decreased E2F1-induced apoptosis in response to etoposide. Thus, we conclude that activation of E1AF provides a means for E2F1 to induce cell apoptosis in response to DNA damage.
Wei
Yuanyan
Y
Key Laboratory of Glycoconjuates Research & Gene Research Center, Shanghai Medical College of Fudan University, Shanghai, People's Republic of China.
Liu
Dan
D
Ge
Yuqing
Y
Zhou
Fengbiao
F
Xu
Jiejie
J
Chen
Hong
H
Gu
Jianxin
J
Jiang
Jianhai
J
D017500:Adenovirus E1A Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D001483:Base Sequence; D002460:Cell Line; D004249:DNA Damage; D017931:DNA Primers / Q000235:genetics; D050687:E2F1 Transcription Factor / Q000378:metabolism*; D005047:Etoposide / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D050783:Proto-Oncogene Proteins c-ets; D034622:RNA Interference
D017500:Adenovirus E1A Proteins; D017931:DNA Primers; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; C079853:ETV4 protein, human; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D005047:Etoposide