Synthesis of glyceryl ferulate by immobilized ferulic acid esterase.
Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL "Amano" from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50 degrees C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25 degrees C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.
Matsuo
Takemasa
T
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Kobayashi
Takashi
T
Kimura
Yukitaka
Y
Tsuchiyama
Moriyasu
M
Oh
Tadanobu
T
Sakamoto
Tatsuji
T
Adachi
Shuji
S
D001234:Aspergillus niger / Q000201:enzymology; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D050178:Monoglycerides / Q000096:biosynthesis*; D011096:Polygalacturonase / Q000378:metabolism; D012995:Solubility; D013696:Temperature
D003373:Coumaric Acids; D004800:Enzymes, Immobilized; D050178:Monoglycerides; C531553:glyceryl ferulate; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D011096:Polygalacturonase; D005990:Glycerol