title: Reverse transcription loop-mediated isothermal amplification for the detection of highly pathogenic porcine reproductive and respiratory syndrome virus.
abstract: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the open reading frames 1a of highly pathogenic porcine reproductive and respiratory syndrome virus genome was developed. The 10 reference strains, 1 clinical isolation strain and 122 positive samples were tested. Positive reactions were confirmed for all strains and specimens by reverse transcription loop-mediated isothermal amplification and nested reverse transcription polymerase chain reaction (RT-PCR). The results showed this detection technique is more reliable and convenient for rapid and sensitive diagnosis of highly pathogenic porcine reproductive and respiratory syndrome virus infection.
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Hao-tai
authors.initials: HT
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: De-hui
authors.initials: DH
authors.lastname: Sun
authors.forename: Li-na
authors.initials: LN
authors.lastname: Ma
authors.forename: Xiang-tao
authors.initials: XT
authors.lastname: Liu
authors.forename: Kai
authors.initials: K
authors.lastname: Quan
authors.forename: Yong-sheng
authors.initials: YS
authors.lastname: Liu
mesh_terms: D000818:Animals; D001769:Blood / Q000821:virology; D008168:Lung / Q000821:virology; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012661:Semen / Q000821:virology; D012680:Sensitivity and Specificity; D013552:Swine; D013997:Time Factors