title: Progesterone regulation of glutathione S-transferase Mu2 expression in mouse uterine luminal epithelium during preimplantation period.
abstract: OBJECTIVE
  To investigate the differential expression and regulation of Gstm2 in mouse uterus during early pregnancy.
  
  
  METHODS
  Experimental animal study.
  
  
  METHODS
  University research laboratory.
  
  
  METHODS
  Sexually mature female Kunming white strain mice.
  
  
  METHODS
  Delayed and activated implantation, pseudopregnancy, hormonal treatment.
  
  
  METHODS
  The expression of Gstm2 mRNA was detected by in situ hybridization and reverse-transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  By in situ hybridization, there were a low level of Gstm2 expression in luminal epithelium on day 3 and a strong level in the luminal epithelium on day 4 during early pregnancy. The expression pattern of Gstm2 in the pseudopregnant uterus was similar to that during early pregnancy. By RT-PCR, Gstm2 was strongly detected in the uteri on days 3 and 4 of pregnancy and pseudopregnancy. Gstm2 expression was strongly detected in the luminal epithelium under delayed implantation, but not seen after delayed implantation was activated by estrogen. In the ovariectomized mouse uterus, Gstm2 expression was strongly up-regulated by progesterone via progesterone receptor.
  
  
  CONCLUSIONS
  The results showed that Gstm2 was highly expressed in the uterine luminal epithelium during preimplantation period and up-regulated by progesterone.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ni
authors.forename: Xing-Jiang
authors.initials: XJ
authors.lastname: Yu
authors.forename: Hua-Jie
authors.initials: HJ
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Lei
authors.forename: Deivendran
authors.initials: D
authors.lastname: Rengaraj
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Li
authors.forename: Zeng-Ming
authors.initials: ZM
authors.lastname: Yang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D010064:Embryo Implantation; D004847:Epithelial Cells / Q000201:enzymology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005982:Glutathione Transferase / Q000187:drug effects / Q000235:genetics*; D017403:In Situ Hybridization; D051379:Mice; D008815:Mice, Inbred Strains; D015735:Mifepristone / Q000494:pharmacology; D010052:Ovariectomy; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects / Q000235:genetics; D011374:Progesterone / Q000494:pharmacology / Q000502:physiology*; D011555:Pseudopregnancy / Q000201:enzymology / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014599:Uterus / Q000201:enzymology*
chemical_list: D015735:Mifepristone; D011374:Progesterone; D004958:Estradiol; D005982:Glutathione Transferase; C412602:glutathione S-transferase Mu 2