title: Beta1,2-xylosyltransferase Cxt1p is solely responsible for xylose incorporation into Cryptococcus neoformans glycosphingolipids.
abstract: The Manalpha1,3(Xylbeta1,2)Manalpha structural motif is common to both capsular polysaccharides of Cryptococcus neoformans and to cryptococcal glycosphingolipids. Comparative analysis of glycosphingolipid structural profiles in wild-type and mutant strains showed that the Xylbeta1,2-transferase (Cxt1p) that participates in capsular polysaccharide biosynthesis is also the sole transferase responsible for adding xylose to C. neoformans glycosphingolipids.
authors.affiliation: Department of Chemistry, University of New Hampshire, G229 Parsons Hall, Durham, New Hampshire 03824-3598, USA.
authors.forename: Sherry A
authors.initials: SA
authors.lastname: Castle
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Owuor
authors.forename: Stephanie H
authors.initials: SH
authors.lastname: Thompson
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Garnsey
authors.forename: J Stacey
authors.initials: JS
authors.lastname: Klutts
authors.forename: Tamara L
authors.initials: TL
authors.lastname: Doering
authors.forename: Steven B
authors.initials: SB
authors.lastname: Levery
mesh_terms: D003455:Cryptococcus neoformans / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006028:Glycosphingolipids / Q000737:chemistry / Q000378:metabolism*; D010430:Pentosyltransferases / Q000235:genetics / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D006028:Glycosphingolipids; D014994:Xylose; D010430:Pentosyltransferases; C408586:beta 1,2-xylosyltransferase