Effects of a casein hydrolysate prepared from Aspergillus oryzae protease on adjuvant arthritis in rats.
We evaluated the effects of a casein hydrolysate (CH) prepared from Aspergillus oryzae protease on rat adjuvant arthritis, a model of human rheumatoid arthritis. CH was administered orally once a day to the animals for 22 d after the adjuvant injection. CH suppressed swelling in the adjuvant-uninjected hind paws, and a higher dose of CH suppressed the increase in arthritic score and swelling of the adjuvant-injected hind paws. A histopathological examination revealed evidence that the higher dose of CH suppressed the articular changes in the rats. In addition, CH suppressed the production of nitric oxide and prostaglandin E(2) in the plasma of the rats. These results suggest that CH had a suppressive effect on adjuvant arthritis by inhibiting the acute and chronic inflammatory reactions.
Hatori
Michio
M
R and D Center, Calpis Co., Ltd., Sagamihara-shi, Kanagawa, Japan.
Ohki
Kohji
K
Hirano
Shin-ichi
S
Yang
Xiao-Ping
XP
Kuboki
Hiroshi
H
Abe
Chiyuki
C
D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D001236:Aspergillus oryzae / Q000235:genetics / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002364:Caseins / Q000096:biosynthesis / Q000235:genetics / Q000627:therapeutic use; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D007052:Ibuprofen / Q000627:therapeutic use; D009569:Nitric Oxide / Q000096:biosynthesis; D051381:Rats
D002364:Caseins; D009569:Nitric Oxide; C475729:casein hydrolysate; D015232:Dinoprostone; D007052:Ibuprofen