Expression of adiponectin receptors, AdipoR1 and AdipoR2, in normal colon epithelium and colon cancer tissue.
Adiponectin is secreted by adipocytes and is a key hormone responsible for insulin sensitization. Recent studies have shown that plasma adiponectin is decreased in patients with breast, endometrial and gastric cancer. However, the effect of adiponectin on colorectal carcinogenesis is controversial. It is now well known that the adiponectin receptor exists in two isoforms, adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2). We examined the expression of the adiponectin receptors on normal colon mucosa and colon cancer tissues in a human study using real-time RT-PCR, Western blotting and immunohistochemical staining. Adiponectin receptors, AdipoR1/AdipoR2, were expressed in normal colon epithelial and colon cancer cells. Furthermore, laser microdissection was performed to confirm our results. These results suggest that adiponectin may exert some effects on normal colon epithelium or colon cancer cells directly through adiponectin receptors. Further studies are required to elucidate the function of the AdipoRs activated by adiponectin and the downstream mechanisms of AdipoRs in colon cancer cells.
Yoneda
Kyoko
K
Gastroenterology Division, Yokohama City University School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama, Japan.
Tomimoto
Ayako
A
Endo
Hiroki
H
Iida
Hiroshi
H
Sugiyama
Michiko
M
Takahashi
Hirokazu
H
Mawatari
Hironori
H
Nozaki
Yuichi
Y
Fujita
Koji
K
Yoneda
Masato
M
Inamori
Masahiko
M
Nakajima
Noriko
N
Wada
Koichiro
K
Nagashima
Yoji
Y
Nakagama
Hitoshi
H
Uozaki
Hitoshi
H
Fukayama
Masashi
M
Nakajima
Atsushi
A
D015153:Blotting, Western; D003106:Colon / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D012333:RNA, Messenger; D054419:Receptors, Adiponectin