Inhibitory effect of antioxidants on hydroxyl radical generation from methylguanidine: an ESR study.
Methylguanidine (MG) is known as not only a nephrotoxin but also as a neurotoxine. We have previously showed that MG itself generates hydroxyl radicals (*OH) in an in vitro study. In this study, we examined the inhibitory effects of ascorbate, EPC-K(1) (alpha-tocopheryl-L-ascorbate-2-O-phosphate diester), Trolox (water-soluble vitamin E analogue), and glutathione (GSH) on *OH generation from MG using an electron spin resonance (ESR) spectrometry with spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). It was found that these compounds have potent inhibitory effect on *OH generation from MG in the order of ascorbate > GSH > EPC-K(1) > Trolox.
Noda
Yasuko
Y
Department of Anti-aging Food Sciences, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Okayama, 700-8558, Japan. yasukon@md.okayama-u.ac.jp
Mankura
Mitsumasa
M
D000975:Antioxidants / Q000737:chemistry*; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D002839:Chromans / Q000737:chemistry; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005978:Glutathione / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry*; D008760:Methylguanidine / Q000737:chemistry*; D019097:Spin Trapping; D014810:Vitamin E / Q000031:analogs & derivatives / Q000737:chemistry
D000975:Antioxidants; D002839:Chromans; D003497:Cyclic N-Oxides; D014810:Vitamin E; D017665:Hydroxyl Radical; D008760:Methylguanidine; C066638:potassium ascorbyl tocopheryl phosphate; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D005978:Glutathione; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid