Disruption of the SCS2 ortholog in the alkane-assimilating yeast Yarrowia lipolytica impairs its growth on n-decane, but does not impair inositol prototrophy.
Disruption of an SCS2 ortholog impaired the growth of the alkane-assimilating yeast Yarrowia lipolytica on n-alkanes, particularly on n-decane, although the mRNA level of the ALK1 gene encoding a highly inducible cytochrome P450ALK was not much affected. The same disruption did not cause inositol auxotrophy, implying that Y. lipolytica SCS2 has a different function from its Saccharomyces cerevisiae counterpart.
Kobayashi
Satoshi
S
Department of Biotechnology, The University of Tokyo, Japan.
Hirakawa
Kiyoshi
K
Fukuda
Ryouichi
R
Ohta
Akinori
A
D000473:Alkanes / Q000378:metabolism*; D000595:Amino Acid Sequence; D007294:Inositol / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D025062:Yarrowia / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D000473:Alkanes; D008565:Membrane Proteins; D012333:RNA, Messenger; D007294:Inositol