Chlorella vulgaris up-modulation of myelossupression induced by lead: the role of stromal cells.
In this study, Chlorella vulgaris (CV) was examined for its chelating effects on the ability of bone marrow stromal cell layer to display myeloid progenitor cells in vitro in lead-exposed mice, using the long-term bone marrow culture (LTBMC). In addition, the levels of interleukin (IL)-6, an important hematopoietic stimulator, as well as the numbers of adherent and non-adherent cells were also investigated. Mice were gavage treated daily with a single 50mg/kg dose of CV for 10 days, concomitant to continuous offering of 1300ppm lead acetate in drinking water. We found that CV up-modulates the reduced ability of stromal cell layer to display myeloid progenitor cells in vitro in lead-exposed mice and restores both the reduced number of non-adherent cells and the ability of stromal cells from these mice to produce IL-6. Monitoring of lead poisoning demonstrated that CV treatment significantly reduced lead levels in blood and tissues, completely restored the normal hepatic ALA levels, decreased the abnormally high plasma ALA and partly recovered the liver capacity to produce porphyrins. These findings provide evidence for a beneficial use of CV for combination or alternative chelating therapy to protect the host from the damage induced by lead poisoning.
Queiroz
Mary L S
ML
Departamento de Farmacologia and Hemocentro, FCM Universidade Estadual de Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil. mlsq@fcm.unicamp.br
Torello
Cristiane O
CO
Perhs
Simone M C
SM
Rocha
Michelle C
MC
Bechara
Etelvino J H
EJ
Morgano
Marcelo A
MA
Valadares
Marize C
MC
Rodrigues
Ana Paula O
AP
Ramos
Aline Lisie
AL
Soares
Chrislaine O
CO
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D002478:Cells, Cultured; D002614:Chelating Agents / Q000737:chemistry* / Q000494:pharmacology*; D048408:Chlorella vulgaris / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002999:Clone Cells / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D015850:Interleukin-6 / Q000378:metabolism; D007854:Lead / Q000097:blood / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011166:Porphyrins / Q000096:biosynthesis; D013234:Stem Cells / Q000187:drug effects; D017154:Stromal Cells / Q000187:drug effects*
D002614:Chelating Agents; D015850:Interleukin-6; D011166:Porphyrins; D007854:Lead