title: Spongiispira norvegica gen. nov., sp. nov., a marine bacterium isolated from the boreal sponge Isops phlegraei.
abstract: The bacterial strain Gp_4_7.1T, isolated from the marine sponge Isops phlegraei collected at the Sula Ridge off the Norwegian coast, was characterized. The isolate was a motile spirillum that was monopolarly and monotrichously flagellated. It was aerobic, Gram-negative, oxidase-positive and catalase-negative. Optimal growth occurred between 20 and 30 degrees C, at pH 7-8 and with a salt concentration of 2-3 % (w/v). The isolate showed a relatively restricted nutritional profile. Substrate utilization tests were only positive for arabinose. Enzyme tests were positive for esterase lipase C8, lipase C14, leucine arylamidase and naphthol-AS-BI-phosphohydrolase. The strain was not able to reduce nitrate. The major cellular fatty acids were C16:1 omega7 and C16:0. The DNA G+C content was 62.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison classified the strain as a member of the order Oceanospirillales in the class Gammaproteobacteria. Strain Gp_4_7.1T formed a distinct phyletic line with less than 94 % 16S rRNA gene sequence similarity to its closest relatives with validly published names. Based on the determined data, it is proposed that the strain represents a novel species in a new genus, Spongiispira norvegica gen. nov., sp. nov.; the type strain of Spongiispira norvegica is Gp_4_7.1T (=DSM 17749T =NCIMB 14401T).
authors.affiliation: Berlin University of Technology, Environmental Microbiology Group, Franklinstrasse 29, Sekr. FR 1-2, Berlin, Germany. ineskaesler@web.de
authors.forename: Ines
authors.initials: I
authors.lastname: Kaesler
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Graeber
authors.forename: Martin S
authors.initials: MS
authors.lastname: Borchert
authors.forename: Thomas
authors.initials: T
authors.lastname: Pape
authors.forename: Ralf
authors.initials: R
authors.lastname: Dieckmann
authors.forename: Hans
authors.initials: H
authors.lastname: von Döhren
authors.forename: Preben
authors.initials: P
authors.lastname: Nielsen
authors.forename: Rudi
authors.initials: R
authors.lastname: Lurz
authors.forename: Walter
authors.initials: W
authors.lastname: Michaelis
authors.forename: Ulrich
authors.initials: U
authors.lastname: Szewzyk
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D020459:Genes, rRNA; D008386:Marine Biology; D008969:Molecular Sequence Data; D009664:Norway; D010641:Phenotype; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S