title: Palmitoylation-dependent endosomal localization of AATYK1A and its interaction with Src.
abstract: Apoptosis-associated tyrosine kinase 1 (AATYK1), also named LMTK1, was previously isolated as an apoptosis-related gene from 32Dcl3 myeloid precursor cells, but its precise function remains unknown. AATYK1A, an isoform without a transmembrane domain, is highly expressed in neurons. We identified palmitoylation of AATYK1A at three N-terminal cysteine residues in cortical cultured neurons and COS-7 cells and found that palmitoylation determined localization of AATYK1A to the transferrin receptor-positive recycling endosomes. Further, we identified the tyrosine kinase Src as a novel AATYK1A-interacting protein. Src and Fyn phosphorylated AATYK1A at tyrosines 25 and 46 in a palmitoylation-dependent manner. The association of AATYK1A with Src in endosomes was also found to be palmitoylation-dependent. These results indicate that palmitoylation is a critical factor not only for the subcellular localization of AATYK1A but also for its interaction with Src.
authors.affiliation: Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan. tutumi-kouji@ed.tmu.ac.jp
authors.forename: Koji
authors.initials: K
authors.lastname: Tsutsumi
authors.forename: Mineko
authors.initials: M
authors.lastname: Tomomura
authors.forename: Teiichi
authors.initials: T
authors.lastname: Furuichi
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Hisanaga
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000145:classification* / Q000502:physiology*; D019556:COS Cells; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D002522:Chlorocebus aethiops; D011992:Endosomes / Q000378:metabolism*; D054878:Lipoylation; D051379:Mice; D009474:Neurons / Q000378:metabolism; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D019061:src-Family Kinases