Essential role of ADAM28 in regulating the proliferation and differentiation of human dental papilla mesenchymal cells (hDPMCs).
Dental papilla mesenchymal cells (DPMCs) have been supposed to possess the relatively independent and critical role for tooth development and morphogenesis. Here, we characterized the role of ADAM28, a member of a disintegrin and metalloproteinase (ADAM) family, in the regulative mechanisms of odontogenic capability of hDPMCs. Immunofluorescence staining showed the ubiquitous expression of ADAM28 in multiple human dental mesenchymal and epithelial cells. After confirming the effect of eukaryotic expression plasmid containing ADAM28 coding region and ADAM28 antisense oligodeoxynucleotide (AS-ODN), we respectively transfected them into hDPMCs and observed the biological markers for proliferation and differentiation. Overexpression of ADAM28 favored the proliferation and lineage-specific differentiation of hDPMCs, while blockage of ADAM28 exerted the opposite effects and induced apoptosis. These results identified an unrecognized hypothesis that ADAM28 may function as positive regulator of growth and differentiation of hDPMCs and act as an important molecule mediating reciprocal epithelial-mesenchymal signaling during tooth organ development.
Zhao
Zheng
Z
Research and Development Center for Tissue Engineering, Fourth Military Medical University, and Department of Otolaryngology, Xijing Hospital, 145 West Changle Road, 710032, Xi'an, China.
Tang
Liang
L
Deng
Zhihong
Z
Wen
Lingying
L
Jin
Yan
Y
D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D003771:Dental Papilla / Q000166:cytology / Q000201:enzymology*; D004847:Epithelial Cells / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008648:Mesoderm / Q000166:cytology / Q000201:enzymology*; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D014162:Transfection
D016376:Oligonucleotides, Antisense; D051722:ADAM Proteins; C410224:ADAM28 protein, human