Towards on-site pathogen detection using antibody-based sensors.
In this paper, the recent progress within biosensors for plant pathogen detection will be reviewed. Bio-recognition layers on sensors can be designed in various ways, however the most popular approach is to immobilise antibodies for specific capture of analytes. Focus will be put on antibody surface-immobilisation strategies as well as the use of antibodies in the widely used sensors, quartz crystal microbalance, surface plasmon resonance and cantilevers. We will describe the available data on antibody-based plant pathogen detection and furthermore use examples from detection of the pathogens Salmonella, Listeria monocytogenes, Streptococcus mutans, Bacillus cereus, Bacillus anthracis, Campylobacter and Escherichia coli. We will touch upon optimal assay design and further discuss the strengths and limitations of current sensor technologies for detection of viruses, bacteria and fungi.
Skottrup
Peter Durand
PD
Department of Micro and Nanotechnology, DTU Nanotech, Technical University of Denmark, Ørsteds Plads, Kgs Lyngby, Denmark. peter.skottrup@mic.dtu.dk
Nicolaisen
Mogens
M
Justesen
Annemarie Fejer
AF
D000907:Antibodies, Bacterial / Q000276:immunology*; D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D010944:Plants / Q000382:microbiology*; D019095:Point-of-Care Systems; D011791:Quartz / Q000737:chemistry; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance
D000907:Antibodies, Bacterial; D011791:Quartz