title: Rapid inhibition of ATP-induced currents by corticosterone in rat dorsal root ganglion neurons.
abstract: The effects of corticosterone (CORT), a natural glucocorticoid hormone, on ATP-induced currents in rat dorsal root ganglion (DRG) neurons and the underlying signaling mechanism were studied by using patch-clamp techniques. Three types of currents (fast, slow and mixed) were evoked by ATP in cultured DRG neurons. Pretreatment with CORT (0.01-10 mumol/l) for 30 s could inhibit the fast current and the fast component of the mixed current. In contrast, CORT had no significant effect on the slow current evoked by ATP. The inhibitory effects were concentration dependent, reversible and could be blocked by glucocorticoid receptor antagonist RU38486 (10 micromol/l), but not by GDP-beta-S (0.2 mmol/l), a blocker of G protein activation. Membrane-impermeable bovine serum albumin-conjugated corticosterone failed to mimic the effects of CORT. The inhibitory effects of CORT on ATP-induced currents diminished after adding protein kinase A inhibitor H89 (10 micromol/l), but were not influenced by protein kinase C inhibitor chelerythrine chloride (10 micromol/l). These results suggest that glucocorticoid hormones might participate in the control of pain by modulating P2X(3) receptor-mediated events in sensory neurons, and the effect is mediated by glucocorticoid receptors and the downstream activation of protein kinase A.
authors.affiliation: Department of Neurobiology, Third Military Medical University, Chongqing, PR China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Liu
authors.forename: Jun-Wei
authors.initials: JW
authors.lastname: Zeng
authors.forename: Yan-Dong
authors.initials: YD
authors.lastname: Zhao
authors.forename: Peng-Hui
authors.initials: PH
authors.lastname: Chen
authors.forename: Zhi
authors.initials: Z
authors.lastname: Xiao
authors.forename: Huai-Zhen
authors.initials: HZ
authors.lastname: Ruan
mesh_terms: D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D003345:Corticosterone / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism; D010146:Pain / Q000378:metabolism; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000187:drug effects* / Q000378:metabolism; D058477:Receptors, Purinergic P2X3; D012710:Serum Albumin, Bovine; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; C546410:P2rx3 protein, rat; D011965:Receptors, Glucocorticoid; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; D012710:Serum Albumin, Bovine; D000255:Adenosine Triphosphate; D003345:Corticosterone