title: First detection of Israeli acute paralysis virus (IAPV) in France, a dicistrovirus affecting honeybees (Apis mellifera).
abstract: Bee samples were collected in French apiaries that displayed severe losses and mortality during the winter (from November 2007 to March 2008). They were screened for the presence of Israeli acute paralysis virus (IAPV) by using RT-PCR. Five out of 35 surveyed apiaries, located in two different geographical areas, were found positive. This represents the first reported detection of IAPV in France. The specificity of the PCR products was checked by sequencing. The phylogenetic analysis showed that French isolates of IAPV were closely related to a cluster including American and Australian isolates. Nevertheless, most of American isolates previously reported to be associated to Colony Collapse Disorder (CCD) and an Israeli isolate first isolated in 2004 from dead bees were included in another cluster. Since IAPV was detected in only 14% of the affected apiaries, it was not possible to establish a causal link between IAPV and the severe winter losses that occurred.
authors.affiliation: Agence Française de Sécurité Sanitaire des Aliments Sophia-Antipolis, Unité Pathologie de I'Abeille, Les Templiers, Route des Chappes, BP 111, 06902 Sophia Antipolis, France. p.blanchard@afssa.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Blanchard
authors.forename: Frank
authors.initials: F
authors.lastname: Schurr
authors.forename: Olivier
authors.initials: O
authors.lastname: Celle
authors.forename: Nicolas
authors.initials: N
authors.lastname: Cougoule
authors.forename: Patrick
authors.initials: P
authors.lastname: Drajnudel
authors.forename: Richard
authors.initials: R
authors.lastname: Thiéry
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Faucon
authors.forename: Magali
authors.initials: M
authors.lastname: Ribière
mesh_terms: D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000821:virology*; D005602:France; D054884:Host-Pathogen Interactions; D007304:Insect Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007557:Israel; D008969:Molecular Sequence Data; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral