Mesoporous silica nanoparticles as a delivery system of gadolinium for effective human stem cell tracking.
The progress of using gadolinium (Gd)-based nanoparticles in cellular tracking lags behind that of superparamagnetic iron oxide (SPIO) nanoparticles in magnetic resonance imaging (MRI). Here, dual functional Gd-fluorescein isothiocyanate mesoporous silica nanoparticles (Gd-Dye@MSN) that possess green fluorescence and paramagnetism are developed in order to evaluate their potential as effective T1-enhancing trackers for human mesenchymal stem cells (hMSCs). hMSCs are labeled efficiently with Gd-Dye@MSN via endocytosis. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes, osteocytes, and chondrocytes, which can still be readily MRI detected. Imaging, with a clinical 1.5-T MRI system and a low incubation dosage of Gd, low detection cell numbers, and short incubation times is demonstrated on both loaded cells and hMSC-injected mouse brains. This study shows that the advantages of biocompatibility, durability, high internalizing efficiency, and pore architecture make MSNs an ideal vector of T1-agent for stem-cell tracking with MRI.
Hsiao
Jong-Kai
JK
Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
Tsai
Chih-Pin
CP
Chung
Tsai-Hua
TH
Hung
Yann
Y
Yao
Ming
M
Liu
Hon-Man
HM
Mou
Chung-Yuan
CY
Yang
Chung-Shi
CS
Chen
Yao-Chang
YC
Huang
Dong-Ming
DM
D002478:Cells, Cultured; D016503:Drug Delivery Systems; D005682:Gadolinium / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008279:Magnetic Resonance Imaging; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013234:Stem Cells / Q000378:metabolism*
D012822:Silicon Dioxide; D005682:Gadolinium