title: Developmental regulation of oligosialylation in zebrafish.
abstract: Zebrafish appears as a relevant model for the functional study of glycoconjugates along vertebrate's development. Indeed, as a prelude to such studies, we have previously identified a vast array of potentially stage-specific glycoconjugates, which structures are reminiscent of glycosylation pathways common to all vertebrates. In the present study, we have focused on the identification and regulation of major protein and lipids associated alpha2-8-linked oligosialic acids motifs in the early development of zebrafish. By a combination of partial hydrolysis, anion exchange HPLC-FD and mass spectrometry, we demonstrated that glycoproteins and glycolipids differed by the extent and the nature of their substituting oligosialylated sequences. Furthermore, relative quantifications showed that alpha2-8-linked sialylation was differentially regulated in both families of glycoconjugates along development. Accordingly, we established that alpha2,8-sialyltransferase mRNA levels was directly correlated with changes of alpha2,8-sialylation status of glycolipids, but independent of those observed on major glycoproteins that appear to originate from the mother.
authors.affiliation: Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576, Université des Sciences et Technologies de Lille 1, 59655, Villeneuve d'Ascq, France.
authors.forename: Lan-Yi
authors.initials: LY
authors.lastname: Chang
authors.forename: Anne
authors.initials: A
authors.lastname: Harduin-Lepers
authors.forename: Ken
authors.initials: K
authors.lastname: Kitajima
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sato
authors.forename: Chang-Jen
authors.initials: CJ
authors.lastname: Huang
authors.forename: Kay-Hooi
authors.initials: KH
authors.lastname: Khoo
authors.forename: Yann
authors.initials: Y
authors.lastname: Guérardel
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000201:enzymology; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D006023:Glycoproteins; D011134:Polysaccharides; D012333:RNA, Messenger; D012799:Sialyltransferases; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid