MYMIV replication initiator protein (Rep): roles at the initiation and elongation steps of MYMIV DNA replication.
In order to explore the mechanism of geminivirus DNA replication, we show that the Replication initiator (Rep) protein encoded by Mungbean yellow mosaic India virus (MYMIV), a member of the family Geminiviridae, binds specifically to the iterons present in the viral DNA replication origin (CR-A) in a highly ordered manner that might be a prerequisite for the initiation of replication. MYMIV Rep also acts as a helicase during the post-initiation stage and is upregulated in presence of the RPA32 subunit of Replication Protein A. The implication of these findings on the initiation and elongation stages of MYMIV DNA replication has been discussed.
Singh
Dharmendra Kumar
DK
Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
Malik
Punjab Singh
PS
Choudhury
Nirupam Roy
NR
Mukherjee
Sunil Kumar
SK
D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication / Q000502:physiology*; D004279:DNA, Viral / Q000096:biosynthesis; D017791:Geminiviridae / Q000235:genetics / Q000502:physiology*; D018741:Replication Origin / Q000502:physiology*; D051716:Replication Protein A / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014779:Virus Replication / Q000235:genetics
D004279:DNA, Viral; D051716:Replication Protein A; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases