title: Promoter polymorphism of cyclooxygenase-1 (COX1) is not associated with ulcerative colitis in the Japanese population.
abstract: OBJECTIVE
  Non-steroidal anti-inflammatory drugs (NSAIDs) aggravate or ameliorate clinical disease activity in patients with ulcerative colitis (UC). UC is associated with increased local production of prostanoids. This study attempted to clarify the relationship between cyclooxygenase-1 (COX1) gene polymorphisms (T-1676C and A-842G/C50T) and ulcerative colitis in a Japanese population.
  
  
  METHODS
  The study was performed on 108 patients with UC (mean age: 39.1 years, M:F=56:52) and 293 healthy controls (mean age: 49.0 years, M:F=161:132). The PCR-SSCP method was employed to detect COX1 polymorphisms using DNA extracted from peripheral blood cells.
  
  
  RESULTS
  No A-842G/C50T polymorphism was detected in all 401 Japanese subjects. The frequency of -1676C allele of COX1 gene in HC group and UC group was 48.8% and 48.1%, respectively. T-1676C genotypes of COX1 did not show significant association with UC risk. In addition, these genotypes were not also associated with the clinicopathological parameters of UC.
  
  
  CONCLUSIONS
  This research suggests that COX1 promoter polymorphisms (T-1676C and A-842G/ C50T) may not be associated with the risk of developing ulcerative colitis in a Japanese population.
authors.affiliation: Department of Gastroenterology, Fujita Health University School of Medicine, Toyoake, Japan. tarisawa@fujita-hu.ac.jp
authors.forename: Tomiyasu
authors.initials: T
authors.lastname: Arisawa
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Tahara
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Nagasaka
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kamiya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujita
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yoshioka
authors.forename: Masaaki
authors.initials: M
authors.lastname: Okubo
authors.forename: Mikiju
authors.initials: M
authors.lastname: Sakata
authors.forename: Fang-Yu
authors.initials: FY
authors.lastname: Wang
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hirata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakano
mesh_terms: D000328:Adult; D000368:Aged; D003093:Colitis, Ulcerative / Q000235:genetics*; D051545:Cyclooxygenase 1 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D051545:Cyclooxygenase 1; C496511:PTGS1 protein, human