title: Isolation and identification of a thermophilic strain producing trehalose synthase from geothermal water in China.
abstract: A slightly thermophilic strain, CBS-01, producing trehalose synthase (TreS), was isolated from geothermal water in this study. According to the phenotypic characteristics and phylogenetic analysis of the 16s rRNA gene sequence, it was identified as Meiothermus ruber. The trehalose synthase gene of Meiothermus ruber CBS-01 was cloned by polymerase chain reaction and sequenced. The TreS gene consisted of 2,895 nucleotides, which specified a 964-amino-acid protein. This novel TreS catalyzed reversible interconversion of maltose and trehalose.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin, PR China.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wei
authors.forename: Yufan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Chen
authors.forename: Laijun
authors.initials: L
authors.lastname: Xing
authors.forename: Mingchun
authors.initials: M
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D002384:Catalysis; D002681:China; D003001:Cloning, Molecular; D034301:Deinococcus / Q000201:enzymology* / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D005964:Glucosyltransferases / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D045482:Hot Springs / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics
chemical_list: D012335:RNA, Ribosomal; D005964:Glucosyltransferases; C099692:trehalose synthase