title: The potential functional interaction of biglycan and WISP-1 in controlling differentiation and proliferation of osteogenic cells.
abstract: Biglycan (BGN) and WISP-1 are 2 extracellular matrix proteins that bind to each other and colocalize in mineralizing tissue. Here we show that WISP-1 abrogates the repression of proliferation in bone marrow stromal cells induced by BGN. We also demonstrate that WISP-1 and its variant WISP-1va can alleviate the repressed osteogenic differentiation caused by the absence of BGN. These preliminary data suggest that WISP-1 and BGN may functionally interact and control each other's activity, thus regulating the differentiation and proliferation of osteogenic cells.
authors.affiliation: Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS Bethesda, Bethesda, Md. 20892-4320, USA.
authors.forename: Colette A
authors.initials: CA
authors.lastname: Inkson
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Ono
authors.forename: Yanming
authors.initials: Y
authors.lastname: Bi
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Kuznetsov
authors.forename: Larry W
authors.initials: LW
authors.lastname: Fisher
authors.forename: Marian F
authors.initials: MF
authors.lastname: Young
mesh_terms: D000818:Animals; D058578:Biglycan; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D055511:CCN Intercellular Signaling Proteins; D002454:Cell Differentiation; D049109:Cell Proliferation; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D010011:Osteocytes / Q000166:cytology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011509:Proteoglycans / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: C546884:BGN protein, human; C546885:Bgn protein, mouse; D058578:Biglycan; D055511:CCN Intercellular Signaling Proteins; C116396:CCN4 protein, human; D016326:Extracellular Matrix Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011509:Proteoglycans; D011518:Proto-Oncogene Proteins; D001973:Bromodeoxyuridine