Gold nanoparticle based NSET for monitoring Mg2+ dependent RNA folding.
Understanding the mechanism of how RNA molecules fold into their native structures are vital to their functional properties. Here we report for the first time that gold nanoparticle based NSET can be used for probing the transition states of an RNA unfolding reaction. Our result shows that time-dependent NSET can clearly distinguish structural transitions between unfolded to folded states. Our experimental observation point out that NSET can be used for the design of an optical based molecular ruler to track RNA folding transition states at distances more than double the distances achievable using traditional dipole-dipole Coulombic energy transfer based methods.
Griffin
Jelani
J
Department of Chemistry, Jackson State University, Jackson, Mississippi 39217-0510, USA.
Ray
Paresh Chandra
PC
D006046:Gold / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry*; D053768:Metal Nanoparticles; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
D012313:RNA; D006046:Gold; D008274:Magnesium