Chromophore-assisted laser inactivation in cell biology.
Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.
Jacobson
Ken
K
Department of Cell and Developmental Biology, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599-7090, USA. frap@med.unc.edu
Rajfur
Zenon
Z
Vitriol
Eric
E
Hahn
Klaus
K
D000818:Animals; D002468:Cell Physiological Phenomena; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D007834:Lasers; D038761:Photobleaching; D055668:Photochemical Processes; D017319:Photosensitizing Agents / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D026082:Singlet Oxygen / Q000737:chemistry
D005456:Fluorescent Dyes; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D049452:Green Fluorescent Proteins; D026082:Singlet Oxygen