[Expression and analysis of the nucleoprotein of paramyxovirus Tianjin strain].
Paramyxovirus Tianjin strain is a novel strain of virus causing common cotton-eared marmoset fatal infection. To investigate the relationship between the gene structure and function of nucleoprotein (NP) of Tianjin strain, NP gene of paramyxovirus Tianjin strain was cloned and three domains of NP were expressed. The homologous and phylogenetic analysis of NP sequences among the paramyxovirus Tianjin strain and eight strains of Sendai viruses from GenBank were performed. The results indicated the recombinant proteins NP1, NP2 and NP3 showed the native antigenicity to the polyclonal antiserum of paramyxovirus Tianjin strain, ranking as NP3>NP1>NP2 (precedence order). The homology of NP nucleotide and the deduced amino acid sequences between paramyxovirus Tianjin strain and Sendai virus BB1 strain were 94.5%, 96.2%, respectively, whereas the identity were 85.1% - 88.7% and 92.4% - 94.7% among Tianjin strain and the 7 strains of Sendai viruses from GenBank respectively. There were 15 unique amino acid substitutions in Tianjin strain NP protein and 11 common amino acid substitutions same with BB1 strain. This research confirmed that paramyxovirus Tianjin strain might be a new genotype of Sendai virus and can be helpful in the establishment of detection assay applying recombinant NP as antigen instead of the whole virions.
Wang
Qing
Q
Department of Microbiology, Tianjin Medical University, Tianjin 300070, China. wangqing_diamond@yahoo.com.cn
Li
Mei
M
Shi
Li-Ying
LY
Yuan
Li-Jun
LJ
Wang
Wen-Xiu
WX
D000595:Amino Acid Sequence; D015153:Blotting, Western; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018098:Paramyxovirinae / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D009698:Nucleoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins