title: Molecular characterization of Echinococcus granulosus in Tunisia and Mauritania by mitochondrial rrnS gene sequencing.
abstract: Cystic hydatid disease is a zoonotic parasitic disease caused by the cestode Echinococcus granulosus and represents a major public health problem in many countries around the world, including North Africa. E. granulosus exists as a series of genetic variants or strains which differ in a wide variety of criteria that impact on the epidemiology, pathology and control of cystic hydatid disease. Nucleotide sequencing of the mitochondrial rrnS gene was here used to characterize 38 E. granulosus isolates collected from different regions and hosts in Tunisia and Mauritania. The results obtained reveal a significant genetic differentiation between E. granulosus hydatid cysts identified as belonging to the G1 genotype and to the G6/G7 cluster using the rrnS gene as marker, and indicate the circulation of the common sheep strain (G1) in all host species from Tunisia and the camel/pig strain cluster (G6/G7) in camel from Mauritania. Other investigations, using this method, are necessary for further genetic analysis of a wider range of isolates from different host species in order to more fully understand the genetic structure of E. granulosus populations and their transmission dynamics in this and neighbouring African countries.
authors.affiliation: Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources UR/09-30, Institut Supérieur de Biotechnologie de Monastir, Tunisie.
authors.forename: S
authors.initials: S
authors.lastname: Farjallah
authors.forename: M
authors.initials: M
authors.lastname: Busi
authors.forename: M Ould
authors.initials: MO
authors.lastname: Mahjoub
authors.forename: B Ben
authors.initials: BB
authors.lastname: Slimane
authors.forename: K
authors.initials: K
authors.lastname: Said
authors.forename: S
authors.initials: S
authors.lastname: D'Amelio
mesh_terms: D000818:Animals; D001483:Base Sequence; D002162:Camelus / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D018509:DNA, Helminth / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004443:Echinococcosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020459:Genes, rRNA; D005838:Genotype; D008435:Mauritania / Q000453:epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018510:RNA, Helminth / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D021521:Ribotyping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000469:parasitology; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology*; D014416:Tunisia / Q000453:epidemiology
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D018510:RNA, Helminth; D012337:RNA, Ribosomal, 18S