title: Amyloid-beta detection with saccharide immobilized gold nanoparticle on carbon electrode.
abstract: The electrochemical sensing of saccharide-protein interactions using a couple of sialic acid derivatives and Alzheimer's amyloid-beta (Abeta) is described. The densely-packed saccharide area for recognition of protein was fabricated onto a carbon electrode by three steps, which were electrochemical deposition of Au nanoparticles on a screen printed strip, self-assembled monolayer (SAM) formation of the acetylenyl group on Au nanoparticles, and the cycloaddition reaction of an azide-terminated sialic acid to the acetylenyl group. The attachment of Abeta peptides to the sialic acid layer was confirmed by electrochemistry and atomic force microscopy imaging. The intrinsic oxidation signal of the captured Abeta(1-40) and (1-42) peptides, containing a single tyrosine (Tyr) residues, was monitored at a peak potential of 0.6 V (vs Ag/AgCl within this sensor) in connection with differential pulse voltammetry. The peak current intensities were concentration dependent. The proposed process provides new routes for analysis of saccharide-protein interactions and electrochemical biosensor development.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi City, Ishikawa 923-1292, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Chikae
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Fukuda
authors.forename: Kagan
authors.initials: K
authors.lastname: Kerman
authors.forename: Koutarou
authors.initials: K
authors.lastname: Idegami
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Miura
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tamiya
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods; D002241:Carbohydrates / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006046:Gold; D053768:Metal Nanoparticles; D011485:Protein Binding; D011506:Proteins; D012794:Sialic Acids
chemical_list: D016229:Amyloid beta-Peptides; D002241:Carbohydrates; D011506:Proteins; D012794:Sialic Acids; D006046:Gold