Isolation and identification of a thermophilic strain producing trehalose synthase from geothermal water in China.
A slightly thermophilic strain, CBS-01, producing trehalose synthase (TreS), was isolated from geothermal water in this study. According to the phenotypic characteristics and phylogenetic analysis of the 16s rRNA gene sequence, it was identified as Meiothermus ruber. The trehalose synthase gene of Meiothermus ruber CBS-01 was cloned by polymerase chain reaction and sequenced. The TreS gene consisted of 2,895 nucleotides, which specified a 964-amino-acid protein. This novel TreS catalyzed reversible interconversion of maltose and trehalose.
Zhu
Yueming
Y
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin, PR China.
Zhang
Jun
J
Wei
Dongsheng
D
Wang
Yufan
Y
Chen
Xiaoyun
X
Xing
Laijun
L
Li
Mingchun
M
D000595:Amino Acid Sequence; D002384:Catalysis; D002681:China; D003001:Cloning, Molecular; D034301:Deinococcus / Q000201:enzymology* / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D005964:Glucosyltransferases / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D045482:Hot Springs / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics
D012335:RNA, Ribosomal; D005964:Glucosyltransferases; C099692:trehalose synthase