[Effects of 17beta-estradiol on alkaline phosphatase and osteoprotegerin expression in human periodontal ligament cells].
OBJECTIVE
To investigate the effects of 17beta-estradiol on the expression of alkaline phosphatase (ALP) and osteoprotegerin in human periodontal ligament cells.


METHODS
Human periodontal ligament cells (hPDLC) were obtained from periodontal tissue explants of teeth extracted for orthodontic treatment ALP activity was determined by PNPP, and OPG protein and corresponding mRNA levels were quantitatively detected by ELISA and RT-PCR RESULTS: ALP activity was significantly increased at 14 days and 21 days (P <0.05). 17beta-E2 of physiological concentration promoted secretion of OPG protein and expression of OPG mRNA (P <0.05). 17beta-E2 with high-dose showed no effect on OPG protein secretion and decrease OPG mRNA expression.


CONCLUSIONS
17beta-E2 may have a positive impact on periodontium through promoting expression of ALP and OPG in hPDLC.
Zhou
Yan
Y
Department of Periodontology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
Fu
Yun
Y
Li
Jing-Ping
JP
Qi
Liu-Ying
LY
D000469:Alkaline Phosphatase / Q000378:metabolism*; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D053244:Osteoprotegerin / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
D053244:Osteoprotegerin; C506030:TNFRSF11B protein, human; D004958:Estradiol; D000469:Alkaline Phosphatase