Micropropagation of Elaeis guineensis Jacq. 'Dura': Comparison of three basal media for efficient regeneration.
Three basal plant tissue culture media, namely, N6, MS, and modified Y3, were compared to optimize micropropagation protocol for E. guineensis. Full strength media were used separately to regenerate plantlets directly using immature zygotic embryos (IZEs), and through somatic embryogenesis of calli obtained from IZEs. The plantlets regenerated by direct regeneration on three media were examined for shoot length and rooting percentage. For the induction of callus, somatic embryogenesis, and rooting modified Y3 medium was the most effective. In conclusion, the results indicate that modified Y3 medium is the most suitable for direct regeneration, callus induction and somatic embryogenesis in E. guineensis.
Muniran
F
F
Melaka Institute of Biotechnology, Melaka International Trade Center City, Lot 7, Ayer Keroh 75450, Melaka, Malaysia.
Bhore
Subhash J
SJ
Shah
Farida H
FH
D002712:Chlorides / Q000737:chemistry; D003470:Culture Media / Q000378:metabolism; D018525:Germination; D007477:Ions; D009821:Oils; D010936:Plant Extracts / Q000378:metabolism*; D018521:Plant Physiological Phenomena; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism*; D012038:Regeneration; D013997:Time Factors
D002712:Chlorides; D003470:Culture Media; D007477:Ions; D009821:Oils; D010936:Plant Extracts