Algorithm and simulation for analysis of bio-images obtained by aperture diffraction based optical MEMS.
This paper proposes a novel method to detect transparent living cells in a transparent microfluidic chamber by optical diffraction of an aperture or an aperture array. Through the analysis of the far-field diffraction pattern, one of the parameters of the cells, including the size, refractive index, or position, can be extracted by the analysis software developed in this paper. Calculations are carried out to discuss the key issues of this MEMS device, and our simulation is verified by diffraction patterns of transparent microparticles on fabricated apertures, recorded via a digital camera.
Zhou
Xiaodong
X
Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602. donna-zhou@imre.a-star.edu.sg
Poenar
Daniel Puiu
DP
Liu
Kai Yu
KY
Tse
Man Siu
MS
Heng
Chew-Kiat
CK
Tan
Swee Ngin
SN
Zhang
Nan
N
D000465:Algorithms; D002478:Cells, Cultured / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012031:Refractometry / Q000379:methods*