title: [Multiple infections of Mycobacterium tuberculosis in treated patients].
abstract: OBJECTIVE
  To identify multiple infections of Mycobacterium tuberculosis in tuberculosis patients.
  
  
  METHODS
  From the clinical isolates of M. tuberculosis during 1999 to 2004 stored at Shanghai Municipal Center for Disease Control and Prevention, cases who had two isolates with different drug resistance patterns in the same treatment episode were selected. The first isolate from the selected patients was inoculated onto Middlebrook 7H11 agar plates, and 30 single colonies from each isolate were picked and genotyped by the 7 loci Variable Number Tandem Repeat (VNTR) method.
  
  
  RESULTS
  Two out of 22 isolates had two different genotypes among their 30 single colonies, and the proportions of the two genotype colonies were 24:6 and 29:1. The other 20 isolates showed identical genotype pattern among their 30 single colonies.
  
  
  CONCLUSIONS
  Multiple infections of M. tuberculosis occurred in tuberculosis patients in Shanghai.
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Southwest University, Chongqing 400716, China.
authors.forename: Ren-Dong
authors.initials: RD
authors.lastname: Fang
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Shen
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Gui
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Gao
authors.forename: Jian
authors.initials: J
authors.lastname: Mei
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial; D005838:Genotype; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D009181:Mycoses / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D015163:Superinfection / Q000382:microbiology*; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial