title: Contrast harmonic imaging characterization of canine splenic lesions.
abstract: BACKGROUND
  Although B-mode ultrasound is very sensitive for the detection of splenic lesions, its specificity is low. Contrast harmonic imaging is used successfully to differentiate benign from malignant liver lesions in humans and dogs.
  
  
  OBJECTIVE
  Contrast harmonic imaging could be useful to differentiate benign and malignant splenic lesions in dogs.
  
  
  METHODS
  Sixty dogs (clinical patients) with splenic abnormalities detected during abdominal ultrasonography.
  
  
  METHODS
  A prospective study was performed with a Philips ATL 5000 unit for contrast pulse inversion harmonic imaging (mechanical index: 0.08, contrast medium: SonoVue). Perfusion was assessed subjectively and quantitatively.
  
  
  RESULTS
  Cytology or histology identified 27 benign (hyperplasia, extramedullary hematopoiesis, hematoma) and 29 malignant (hemangiosarcoma, malignant lymphoma, malignant histiocytosis, mesenchymal tumors without classification, mast cell tumors, and others) lesions and 4 normal spleens. Except for 1 benign nodule, extensive to moderate hypoechogenicity was only seen in malignant lesions during wash-in, at peak enhancement, and during wash-out (P= .0001, odds ratios: 37.9 [95% CI 4.5-316.5], 66.4 [95% CI 8.0-551.1], and 36.9 [95% CI 4.4-308.4]). Although all but 1 benign lesion enhanced well and were mildly hypo-, iso-, or hyperechoic in comparison with the normal spleen during all blood pool phases, marked enhancement occurred both in benign as well as in malignant splenic lesions. Quantitative perfusion values did not differ significantly between benign and malignant lesions.
  
  
  CONCLUSIONS
  Moderate to extensive hypoechogenicity clearly identifies canine splenic malignant lesions. In nodules with marked enhancement, contrast harmonic ultrasound is of limited value and histology is needed.
authors.affiliation: Vetsuisse Faculty, University of Zurich, Section of Diagnostic Imaging and Radio-Oncology, Winterthurerstrasse, Zurich, Switzerland. sohlerth@vetclinics.uzh.ch
authors.forename: S
authors.initials: S
authors.lastname: Ohlerth
authors.forename: M
authors.initials: M
authors.lastname: Dennler
authors.forename: E
authors.initials: E
authors.lastname: Rüefli
authors.forename: B
authors.initials: B
authors.lastname: Hauser
authors.forename: V
authors.initials: V
authors.lastname: Poirier
authors.forename: N
authors.initials: N
authors.lastname: Siebeck
authors.forename: M
authors.initials: M
authors.lastname: Roos
authors.forename: B
authors.initials: B
authors.lastname: Kaser-Hotz
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000494:pharmacology*; D004283:Dog Diseases / Q000000981:diagnostic imaging*; D004285:Dogs; D005260:Female; D008297:Male; D013158:Splenic Diseases / Q000000981:diagnostic imaging / Q000473:pathology / Q000662:veterinary*; D014463:Ultrasonography
chemical_list: D003287:Contrast Media