title: Chemical constituents of Acanthus ilicifolius L. and effect on osteoblastic MC3T3E1 cells.
abstract: A new coumaric acid derivative called acancifoliuside (1) and six known compounds as acteoside (2), isoacteoside (3), acanthaminoside (4), (+)-lyoniresinol 3a-O-beta-glucopyranoside (5), (-)-lyoniresinol (6), and alpha-amyrin (7), were isolated from the methanolic extract of the leaves of Acanthus ilicifolius L. (Acanthaceae). Their structures were determined by spectroscopic methods and a comparison with the spectral data reported in the literature. The effects of the compounds on the function of osteoblastic MC3T3-E1 cells were tested. Compounds 2, 3, and 5 (30 microM) increased the growth and differentiation of osteoblasts significantly (P<0.05), indicating that A. ilicifolius leaves may help prevent osteoporosis.
authors.affiliation: Institute of Natural Products Chemistry, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Vietnam.
authors.forename: Phan
authors.initials: P
authors.lastname: Van Kiem
authors.forename: Tran Hong
authors.initials: TH
authors.lastname: Quang
authors.forename: Tran Thu
authors.initials: TT
authors.lastname: Huong
authors.forename: Le Thi Hong
authors.initials: le TH
authors.lastname: Nhung
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Cuong
authors.forename: Chau
authors.initials: C
authors.lastname: Van Minh
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Choi
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D016475:3T3 Cells; D027901:Acanthaceae / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003094:Collagen / Q000378:metabolism; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008968:Molecular Conformation; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D050071:Bone Density Conservation Agents; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; D003094:Collagen; D000469:Alkaline Phosphatase; C022616:thiazolyl blue; D002118:Calcium