Identification and characterization of PNRC splicing variants.
Nuclear receptor (NR) dependent transcriptional action requires recruitment of diverse factors characterized as coregulators. PNRC (proline-rich nuclear receptor coregulatory protein) is a member of coregulators that are capable of potentiating the transcriptional activity of NRs. Here we identified three human PNRC splicing variants designated PNRC1c, PNRC1d and PNRC1f. PNRC1c and PNRC1f are generated through alternative recognition of the 3'-splice site in exon 1, leading to in-frame deletion of 79 amino acids (aa) and an altered reading frame, respectively. PNRC1d is generated through the alternate promoter usage and forms a truncated protein containing C-terminus 142 aa of full-length PNRC. These isoforms differ in their abilities to bind NRs and potentiate NR mediated transcriptions. Moreover, PNRC1d can modulate the activity of full-length PNRC in enhancing ER mediated transcription. Our results suggest that PNRC exists as functionally distinct isoforms and alternative splicing serves as a regulatory mechanism of PNRC coactivator activity.
Wang
Yuanzhong
Y
Department of Biochemistry and Molecular Biology, Third Military Medical University, Chonqing, 400038, PR China.
Li
Yuping
Y
Chen
Bin
B
Zhang
Yan
Y
Lou
Guiyu
G
Chen
Shiuan
S
Zhou
Dujin
D
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D019295:Computational Biology; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D000072260:RNA Splicing Factors; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C096395:PNRC1 protein, human; D020033:Protein Isoforms; D000072260:RNA Splicing Factors; D018160:Receptors, Cytoplasmic and Nuclear; D011960:Receptors, Estrogen; C107467:SF1 protein, human; D014157:Transcription Factors