Synthesis and biological evaluation of a polysialic acid-based hydrogel as enzymatically degradable scaffold material for tissue engineering.
Restorative medicine has a constant need for improved scaffold materials. Degradable biopolymers often suffer from uncontrolled chemical or enzymatic hydrolysis by the host. The need for a second surgery on the other hand is a major drawback for nondegradable scaffold materials. In this paper we report the design and synthesis of a novel polysialic acid-based hydrogel with promising properties. Hydrogel synthesis was optimized and enzymatic degradation was studied using a phage-born endosialidase. After addition of endosialidase, hydrogels readily degraded depending on the amount of initially used cross-linker within 2 to 11 days. This polysialic acid hydrogel is not cytotoxic, completely stable under physiological conditions, and could be evaluated as growth support for PC12 cells. Here, additional coating with collagen I, poly-L-lysine or matrigel is mandatory to improve the properties of the material.
Berski
Silke
S
Institut für Organische Chemie and Zentrum für Biomolekulare Wirkstoffe (BMWZ), Gottfried Willhelm Leibniz Universität Hannover, Schneiderberg 1B, 30167 Hannover, Germany.
van Bergeijk
Jeroen
J
Schwarzer
David
D
Stark
Yvonne
Y
Kasper
Cornelia
C
Scheper
Thomas
T
Grothe
Claudia
C
Gerardy-Schahn
Rita
R
Kirschning
Andreas
A
Dräger
Gerald
G
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry; D004338:Drug Combinations; D004591:Electrophoresis, Polyacrylamide Gel; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D007797:Laminin / Q000737:chemistry; D008968:Molecular Conformation; D009439:Neuraminidase / Q000378:metabolism*; D016716:PC12 Cells; D011107:Polylysine / Q000737:chemistry; D011509:Proteoglycans / Q000737:chemistry; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; D004338:Drug Combinations; D020100:Hydrogels; D007797:Laminin; D011509:Proteoglycans; D012794:Sialic Acids; C021319:polysialic acid; C059485:matrigel; D011107:Polylysine; D003094:Collagen; C035355:endo-N-acetylneuraminidase; D009439:Neuraminidase