Cryptic peptide derived from the rat neuropeptide FF precursor affects G-proteins linked to opioid receptors in the rat brain.
Recently, we reported the discovery of a novel amino acid sequence derived from the NPFF precursor NAWGPWSKEQLSPQA, which blocked the expression of conditioned place preference induced by morphine and reversed the antinociceptive activity of morphine (5mg/kg, s.c.) in the tail-immersion test in rats. Here, we name it as NPNA (Neuropeptide NA from its flanking amino acid residues). The synthetic peptide influenced the expression of mRNA coding for Galpha(i1), (i2), and (i3) subunits. The results provide further evidence that yet another bioactive sequence might be present within the NPFF precursor.
Suder
Piotr
P
Department of Neurobiochemistry, Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Ingardena 3 Street, 30-060 Krakow, Poland. suder@chemia.uj.edu.pl
Nawrat
Dominika
D
Bielawski
Adam
A
Zelek-Molik
Agnieszka
A
Raoof
Hana
H
Dylag
Tomasz
T
Kotlinska
Jolanta
J
Nalepa
Irena
I
Silberring
Jerzy
J
D000595:Amino Acid Sequence; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D051960:GTP-Binding Protein alpha Subunit, Gi2 / Q000096:biosynthesis; D044385:GTP-Binding Protein alpha Subunits / Q000096:biosynthesis*; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000096:biosynthesis; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009020:Morphine / Q000037:antagonists & inhibitors; D009479:Neuropeptides / Q000494:pharmacology*; D010147:Pain Measurement; D010446:Peptide Fragments / Q000494:pharmacology*; D011498:Protein Precursors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D044385:GTP-Binding Protein alpha Subunits; D009479:Neuropeptides; D010446:Peptide Fragments; D011498:Protein Precursors; D012333:RNA, Messenger; C539768:neuropeptide FF precursor (85-99), rat; D009020:Morphine; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go