title: Iron dictates the virulence of Pseudomonas aeruginosa in urinary tract infections.
abstract: Iron-limiting conditions have been reported to be prevalent in the milieu of urinary tract. In the present investigation, effect of iron on virulence of uropathogenic Pseudomonas aeruginosa in planktonic and biofilm cell mode was studied. Significant enhancement in elaboration of all the virulence traits along with increased adherence to uroepithelial cells and decreased phagocytosis of P. aeruginosa was observed following growth in iron-deplete medium. On the contrary, decrease in all these parameters except phagocytosis was observed when P. aeruginosa was grown in iron-rich medium. In vivo, P. aeruginosa grown in iron-deplete medium showed increased renal bacterial load and tissue pathology in a mouse model of ascending urinary tract infection compared with organisms grown in iron-replete medium. The results of the present study may help in understanding host-parasite interaction and in developing alternative preventive approach against P. aeruginosa induced urinary tract infections.
authors.affiliation: Division of Infectious Diseases, MS#51, Childrens Hospital Los Angeles, 4650 Sunset Boulevard, Los Angeles, CA 90027, USA. ramittal@chla.usc.edu
authors.forename: Rahul
authors.initials: R
authors.lastname: Mittal
authors.forename: Saroj
authors.initials: S
authors.lastname: Sharma
authors.forename: Sanjay
authors.initials: S
authors.lastname: Chhibber
authors.forename: Kusum
authors.initials: K
authors.lastname: Harjai
mesh_terms: D000818:Animals; D003470:Culture Media; D005260:Female; D006801:Humans; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D007668:Kidney / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000382:microbiology; D051379:Mice; D011552:Pseudomonas Infections / Q000382:microbiology* / Q000473:pathology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D012720:Severity of Illness Index; D013997:Time Factors; D001743:Urinary Bladder / Q000473:pathology; D014552:Urinary Tract Infections / Q000382:microbiology* / Q000473:pathology; D014774:Virulence / Q000276:immunology / Q000502:physiology*; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D003470:Culture Media; D037521:Virulence Factors; D007501:Iron