title: Heterologous protective immunization elicited in mice by Pasteurella multocida fur ompH.
abstract: Different strategies have been developed to produce vaccines against Pasteurella multocida. The approach described herein involves overexpression on the bacterial cell surface of Fur-regulated IROMPs (iron-regulated outer-membrane proteins). Accordingly, the ability of fur mutants to promote heterologous protection was examined in a Swiss mouse animal model. Twofur mutants derived from P. multocida were isolated, one of which was also defective in the OmpH protein. In mice challenged with virulent P. multocida, outer-membrane protein (OMP) extracts of fur cells conferred the same protection as obtained with wild-type cells grown in iron-depleted medium. Total protection was achieved with 40 microg of OMP extract from the fur ompH mutant. Mice administered heat-inactivated fur ompH cells were 60% cross-protected. The presence of a galE mutation in these cells did not further increase the protection level. Additionally, cell disruption by sonication provoked a higher level of protection than conferred by heat-treated cells. Taken together, the results showed that P. multocida fur ompH cells offer a simple and suitable approach for cross-protecting animals against infection with P. multocida.
authors.affiliation: Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain.
authors.forename: M Elena
authors.initials: ME
authors.lastname: Garrido
authors.forename: Montserrat
authors.initials: M
authors.lastname: Bosch
authors.forename: Anna
authors.initials: A
authors.lastname: Bigas
authors.forename: Ignacio
authors.initials: I
authors.lastname: Badiola
authors.forename: Jordi
authors.initials: J
authors.lastname: Barbé
authors.forename: Montserrat
authors.initials: M
authors.lastname: Llagostera
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics*; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D005260:Female; D007274:Injections, Intraperitoneal; D051379:Mice; D009154:Mutation; D010326:Pasteurella Infections / Q000517:prevention & control*; D016979:Pasteurella multocida / Q000276:immunology* / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D012097:Repressor Proteins; D014614:Vaccines, Synthetic; C068477:ferric uptake regulating proteins, bacterial