The lectin-like activity of human C1q and its implication in DNA and apoptotic cell recognition.
C1q, the binding subunit of the C1 complex of complement, is an archetypal pattern recognition molecule known for its striking ability to recognize a wide variety of targets, ranging from pathogenic non self to altered self. DNA is one of the C1q ligands, but the precise region of C1q and the DNA motifs that support interaction have not been characterized yet. Here, we report for the first time that the peripheral globular region of the C1q molecule displays a lectin-like activity, which contributes to DNA binding through interaction with its deoxy-d-ribose moiety and may participate in apoptotic cell recognition.
Païdassi
Helena
H
Institut de Biologie Structurale Jean-Pierre Ebel, F-38027 Grenoble Cedex 1, France.
Tacnet-Delorme
Pascale
P
Lunardi
Thomas
T
Arlaud
Gérard J
GJ
Thielens
Nicole M
NM
Frachet
Philippe
P
D017209:Apoptosis; D001665:Binding Sites; D015922:Complement C1q / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D006601:Hexoses / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D010429:Pentoses / Q000737:chemistry / Q000378:metabolism
D006601:Hexoses; D037102:Lectins; D010429:Pentoses; D015922:Complement C1q; D004247:DNA