Carboxymethyl poly(L-histidine) as a new pH-sensitive polypeptide to enhance polyplex gene delivery.
Carboxymethyl poly( l-histidine) (CM-PLH) as a new pH-sensitive polypeptide has enhanced polyplex gene delivery. Agarose gel retardation assay and zeta potential measurement proved that the anionic CM-PLH at physiological pH coated the PEI/DNA binary complexes. The resulting CM-PLH/PEI/DNA ternary complexes showed the gene expression value 300 times higher than that of the PEI/DNA binary complexes. These results suggest that the synergistic effect of the pH-sensitive imidazole groups at endosomal pH and the anionic carboxymethyl groups at physiological pH in the CM-PLH enhanced polyplex gene delivery.
Asayama
Shoichiro
S
Department of Applied Chemistry, Tokyo Metropolitan University, Hacioji, Tokyo, Japan. asayama-shoichiro@c.metro-u.ac.jp
Sudo
Miyuki
M
Nagaoka
Shoji
S
Kawakami
Hiroyoshi
H
D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D004705:Endocytosis; D011992:Endosomes / Q000737:chemistry; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008247:Lysosomes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
D004337:Drug Carriers; D010455:Peptides; D011506:Proteins; C533250:carboxymethyl polyhistidine; D006639:Histidine; D004247:DNA; D008156:Luciferases