title: Cryptic peptide derived from the rat neuropeptide FF precursor affects G-proteins linked to opioid receptors in the rat brain.
abstract: Recently, we reported the discovery of a novel amino acid sequence derived from the NPFF precursor NAWGPWSKEQLSPQA, which blocked the expression of conditioned place preference induced by morphine and reversed the antinociceptive activity of morphine (5mg/kg, s.c.) in the tail-immersion test in rats. Here, we name it as NPNA (Neuropeptide NA from its flanking amino acid residues). The synthetic peptide influenced the expression of mRNA coding for Galpha(i1), (i2), and (i3) subunits. The results provide further evidence that yet another bioactive sequence might be present within the NPFF precursor.
authors.affiliation: Department of Neurobiochemistry, Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Ingardena 3 Street, 30-060 Krakow, Poland. suder@chemia.uj.edu.pl
authors.forename: Piotr
authors.initials: P
authors.lastname: Suder
authors.forename: Dominika
authors.initials: D
authors.lastname: Nawrat
authors.forename: Adam
authors.initials: A
authors.lastname: Bielawski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zelek-Molik
authors.forename: Hana
authors.initials: H
authors.lastname: Raoof
authors.forename: Tomasz
authors.initials: T
authors.lastname: Dylag
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kotlinska
authors.forename: Irena
authors.initials: I
authors.lastname: Nalepa
authors.forename: Jerzy
authors.initials: J
authors.lastname: Silberring
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D051960:GTP-Binding Protein alpha Subunit, Gi2 / Q000096:biosynthesis; D044385:GTP-Binding Protein alpha Subunits / Q000096:biosynthesis*; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000096:biosynthesis; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009020:Morphine / Q000037:antagonists & inhibitors; D009479:Neuropeptides / Q000494:pharmacology*; D010147:Pain Measurement; D010446:Peptide Fragments / Q000494:pharmacology*; D011498:Protein Precursors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D044385:GTP-Binding Protein alpha Subunits; D009479:Neuropeptides; D010446:Peptide Fragments; D011498:Protein Precursors; D012333:RNA, Messenger; C539768:neuropeptide FF precursor (85-99), rat; D009020:Morphine; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go