Placental Alpha Hemoglobin Stabilizing Protein (AHSP) and recurrent miscarriage.
AHSP inhibits cellular production of the reactive oxygen species. Reduced AHSP indicates reduced protection against oxidative stressors. Our objective was to investigate AHSP levels in recurrent miscarriage (RM). Trophoblast was collected from women of 10 weeks gestation: voluntary abortion controls (VA, n = 10); spontaneous first miscarriage with subsequent normal pregnancy (SMSN, n = 15) or with subsequent miscarriage (SMSM, n = 5); RM previously investigated (RMPS, n = 5) or not previously investigated (RM, n = 5). AHSP mRNA and protein were determined using real-time quantitative polymerase chain reaction (PCR) and Western blot, respectively. One-way ANOVA was performed to assess statistical significance (p < 0.05). ahsp mRNA levels were maximally reduced in RM and RMPS (8.0 x 10(-6) +/- 1.3 and 8.1 x 10(-6) +/- 0.7, respectively) compared with SMSN and VA (16.1 x 10(-6) +/- 2.3 and 26.1 x 10(-6) +/- 2.7, respectively). SMSM showed levels significantly reduced as well (9.0 x 10(-6) +/- 2.3). In RM, a reduced defense from oxidative stressors is evident at first miscarriage, identifying women at high risk for subsequent eventful pregnancy. Reduced AHSP may identify women at risk of experiencing further miscarriages.
Emanuelli
Monica
M
Institute for Biochemical Biotechnologies, Università Politecnica delle Marche, Ancona, Italy.
Cecati
Monia
M
Sartini
Davide
D
Stortoni
Piergiorgio
P
Corradetti
Alessandra
A
Giannubilo
Stefano R
SR
Turi
Angelo
A
Tranquilli
Andrea L
AL
D000026:Abortion, Habitual / Q000378:metabolism*; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
C468768:AHSP protein, human; D001798:Blood Proteins; D018832:Molecular Chaperones; D012333:RNA, Messenger