Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191.
Studies on the DNA-binding properties of transcription factors are important in searching for the downstream genes regulated by these factors. In the present study, we report on the DNA-binding property of a Cys2His2-type transcription factor, zinc finger protein 191 (Zfp191), which has been newly found to play a significant role in mice. By constructing a fusion protein containing the DNA-binding domain of Zfp191, we characterized target DNA by determining the protein's binding specificity and dependence on zinc. The data showed that the DNA-binding domain of Zfp191 can specifically bind to the TCAT repeat motif and that there is a cooperative effect among the target DNA's multiple binding sites. Furthermore, the binding reaction is dependent on zinc. This work provides a foundation for further studies on the role of Zfp191 in gene regulation and development.
Wang
Haoyue
H
Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China.
Sun
Ruilin
R
Liu
Guoxiang
G
Yao
Minghui
M
Fei
Jian
J
Shen
Hebai
H
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D018895:Microsatellite Repeats; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016335:Zinc Fingers
D002352:Carrier Proteins; D017931:DNA Primers; D011993:Recombinant Fusion Proteins; C474810:Zfp24 protein, mouse; D004247:DNA