"Macromolecules to PDMS transfer" as a general route for PDMS biochips.
"Macromolecules to PDMS transfer" technique relying on the direct entrapment of macromolecules spots during PDMS polymerisation is proposed as an alternative for the easy and simple PDMS surface modification. In the present work, the development of three different applications based on this procedure is presented as proof of the method potentialities. First, C-reactive protein (CRP) sandwich immunoassay using immobilised monoclonal anti-CRP antibodies was developed for sepsis diagnosis. The preserved integrity of the immobilised monoclonal immunoglobulin permitted the sensitive detection of free CRP in human sera (LOD=12.5 microg/L, detection ranging over two decades). Then, rheumatoid arthritis diagnosis through the rheumatoid factor (RF) detection based on rabbit immunoglobulins immobilisation allowed the detection of specific antibodies in human sera samples down to low RF levels (detection range 5.3-485 IU/mL). Finally, the "Macromolecules to PDMS transfer" procedure was used to easily and rapidly produce fibronectin-based cell culture arrays. The successful attachment of HeLa and BALB/3T3 cells was demonstrated with optical microscopy and specific staining of actin and vinculin.
Heyries
Kevin A
KA
Laboratoire de Génie Enzymatique et Biomoléculaire, Université Lyon 1, CNRS 5246 ICBMS, Bât CPE 43, bd du 11 novembre 1918, 69622 Villeurbanne Cedex, France.
Mandon
Céline A
CA
Ceriotti
Laura
L
Ponti
Jessica
J
Colpo
Pascal
P
Blum
Loïc J
LJ
Marquette
Christophe A
CA
D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D046912:Multiprotein Complexes / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004129:Dimethylpolysiloxanes; D046912:Multiprotein Complexes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer