Antimalarial activity of natural product extracts from Papua New Guinean and Australian plants against Plasmodium falciparum.
In the search for new antimalarial compounds, a subset of a natural product extract library prepared from plant samples collected from Papua New Guinea and Australia was screened for in vitro activity against the chloroquine-sensitive 3D7 and chloroquine-resistant Dd2 strains of Plasmodium falciparum. Using the incorporation of ((3)H)-hypoxanthine into parasite nucleic acid as a marker of growth, 93 of the 794 extracts screened displayed >40% inhibition against 3D7 infected erythrocytes at 312 microge/mL. Antimalarial activity was confirmed in 48 of these extracts against both 3D7 and Dd2 infected erythrocytes at concentrations between 78 and 390 microge/mL, 14 of which caused >90% growth inhibition of 3D7 at the lowest concentration screened. Extracts were also tested for mammalian cell cytotoxicity to evaluate selectivity of action.
Fernandez
Liza S
LS
Discovery Biology, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, Australia.
Jobling
Michael F
MF
Andrews
Katherine T
KT
Avery
Vicky M
VM
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D004912:Erythrocytes / Q000469:parasitology; D010936:Plant Extracts / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development
D000962:Antimalarials; D001688:Biological Products; D010936:Plant Extracts