title: Integration site preference of xenotropic murine leukemia virus-related virus, a new human retrovirus associated with prostate cancer.
abstract: Xenotropic murine leukemia virus-related virus (XMRV) is a new human gammaretrovirus identified in prostate cancer tissue from patients homozygous for a reduced-activity variant of the antiviral enzyme RNase L. Neither a casual relationship between XMRV infection and prostate cancer nor a mechanism of tumorigenesis has been established. To determine the integration site preferences of XMRV and the potential risk of proviral insertional mutagenesis, we carried out a genome-wide analysis of viral integration sites in the prostate cell line DU145 after an acute XMRV infection and compared the integration site pattern of XMRV with those found for murine leukemia virus and two human retroviruses, human immunodeficiency virus type 1 and human T-cell leukemia virus type 1. Among all retroviruses analyzed, XMRV has the strongest preference for transcription start sites, CpG islands, DNase-hypersensitive sites, and gene-dense regions; all are features frequently associated with structurally open transcription regulatory regions of a chromosome. Analyses of XMRV integration sites in tissues from prostate cancer patients found a similar preference for the aforementioned chromosomal features. Additionally, XMRV integration sites in cancer tissues were associated with cancer breakpoints, common fragile sites, microRNA, and cancer-related genes, suggesting a selection process that favors certain chromosomal integration sites. In both acutely infected cells and cancer tissues, no common integration site was detected within or near proto-oncogenes or tumor suppressor genes. These results are consistent with a model in which XMRV may contribute to tumorigenicity via a paracrine mechanism.
authors.affiliation: Biomedical Engineering Interdepartmental Program, UCLA School of Medicine, Los Angeles, CA 90095, USA.
authors.forename: Sanggu
authors.initials: S
authors.lastname: Kim
authors.forename: Namshin
authors.initials: N
authors.lastname: Kim
authors.forename: Beihua
authors.initials: B
authors.lastname: Dong
authors.forename: David
authors.initials: D
authors.lastname: Boren
authors.forename: Serena A
authors.initials: SA
authors.lastname: Lee
authors.forename: Jaydip
authors.initials: J
authors.lastname: Das Gupta
authors.forename: Christina
authors.initials: C
authors.lastname: Gaughan
authors.forename: Eric A
authors.initials: EA
authors.lastname: Klein
authors.forename: Christopher
authors.initials: C
authors.lastname: Lee
authors.forename: Robert H
authors.initials: RH
authors.lastname: Silverman
authors.forename: Samson A
authors.initials: SA
authors.lastname: Chow
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D018899:CpG Islands; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism; D017988:Gammaretrovirus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000235:genetics / Q000821:virology*; D012045:Regulatory Sequences, Nucleic Acid; D012192:Retroviridae Infections / Q000378:metabolism; D014158:Transcription, Genetic; D016662:Virus Integration
chemical_list: D035683:MicroRNAs; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease