title: Presence of neuropeptide Y in somatotrophs of cattle.
abstract: Neuropeptide Y (NPY), a 36-amino acid member of the pancreatic polypeptide family, was found to be present by immunohistochemistry in the bovine adenohypophysis. NPY mRNA expression was confirmed in the adenohypophysis by RT-PCR. NPY immunoreactivity was present in about 38% of adenohypophyseal cells in the pars distalis. However, NPY immunoreactive cells (NPY-ir cells) were scarce in the zona tuberalis. Immunohistochemistry of NPY and specific hormones using mirror sections revealed that NPY was colocalized in GH immunoreactive cells. Over 90% of somatotrophs corresponded to NPY-ir cells. These results indicate that endogenous NPY is present in the bovine somatotroph and may act as an endocrine intercellular mediator in the adenohypophysis.
authors.affiliation: Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ogasawara
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nagai
authors.forename: Kazunori
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okamura
authors.forename: Sachi
authors.initials: S
authors.lastname: Tanaka
authors.forename: Kouichi
authors.initials: K
authors.lastname: Watanabe
authors.forename: Shyuichi
authors.initials: S
authors.lastname: Ohwada
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D009478:Neuropeptide Y / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D052683:Somatotrophs / Q000166:cytology / Q000378:metabolism*
chemical_list: D009478:Neuropeptide Y; D012333:RNA, Messenger