Taenia crassiceps: energetic and respiratory metabolism from cysticerci exposed to praziquantel and albendazole in vitro.
Praziquantel prevents glucose uptake, influencing energy metabolism, while albendazole selectively inhibits the uptake of glucose, leading to glycogen storage depletion in the parasite. The objective of this study was to determine the concentrations of glucose and organic acids related to energy and respiratory metabolisms in in vitro Taenia crassiceps cysticerci in the initial, larval and final stages exposed to sub lethal dosages of anti-helminthic drugs. Spectrophotometric and chromatographic analysis were performed to detect glucose, lactate, oxaloacetate, citrate, malate, fumarate and succinate secreted/excreted by in vitro cysticerci with 0.03 and 0.06 microg/mL of praziquantel and 0.05 and 0.075 microg/mL of albendazole. The anti-helminthic drugs decreased the excretion of lactate and induced aerobic energy pathways. Concentrations of glucose remained unaltered confirming blockage of its uptake.
Vinaud
Marina Clare
MC
Tropical Pathology and Public Health Institute at the Federal University of Goias, Anapolis, 74605-050 Goias, Brazil. mvinaud@yahoo.com.br
Ferreira
Cirlane Silva
CS
Lino Junior
Ruy de Souza
Rde S
Bezerra
José Clecildo Barreto
JC
D015766:Albendazole / Q000494:pharmacology*; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D002264:Carboxylic Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003552:Cysticercus / Q000187:drug effects* / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects; D005947:Glucose / Q000032:analysis; D019344:Lactic Acid / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011223:Praziquantel / Q000494:pharmacology*; D012143:Respiratory Physiological Phenomena / Q000187:drug effects; D013053:Spectrophotometry
D000871:Anthelmintics; D002264:Carboxylic Acids; D019344:Lactic Acid; D011223:Praziquantel; D015766:Albendazole; D005947:Glucose