Biochemical and behavioral effects of diazinon exposure in hybrid striped bass.
The effects of environmental stimuli on biochemical processes may influence behavior. Environmental contaminants that alter behavior can have major impacts on populations as well as community structures by changing species' interactions. One important behavior is the ability to capture prey. We hypothesized that sublethal exposure to diazinon, an organophosphate pesticide, may lead to feeding behavior abnormalities in hybrid striped bass (Morone saxatilis x M. chrysops) through inhibition of brain acetylcholinesterase (AChE) activity. This can potentially reduce the organism's survival by affecting its ability to find and capture food. To test this hypothesis, bass were exposed to diazinon for 6 d, followed by a 6-d recovery period in clean water. Brain AChE activity and the ability of bass to capture prey fathead minnows (Pimephales promelas) were measured every third day. Exposed fish exhibited a concentration- and duration-dependent decrease in ability to capture prey. While bass in all diazinon treatment groups had significantly inhibited brain AChE activity, only the medium and high treatment groups showed a dose- and time-dependent increase in time to capture prey. Acetylcholinesterase activity also decreased in an exposure duration- and concentration-dependent manner. The AChE levels in exposed fish did not recover to control levels during the 6-d recovery period. These results suggest that sublethal exposure to AChE-inhibiting substances may decrease the ecological fitness of hybrid striped bass by reducing their ability to capture prey.
Gaworecki
Kristen M
KM
Institute of Environmental Toxicology, Department of Biological Sciences, Clemson University, 509 Westinghouse Road, Pendleton, South Carolina 29670, USA. kgawore@clemson.edu
Roberts
Aaron P
AP
Ellis
Norman
N
Sowers
Anthony D
AD
Klaine
Stephen J
SJ
D000110:Acetylcholinesterase / Q000187:drug effects; D000818:Animals; D001492:Bass / Q000502:physiology*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000633:toxicity; D003976:Diazinon / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005247:Feeding Behavior / Q000187:drug effects; D010575:Pesticides / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D002800:Cholinesterase Inhibitors; D010575:Pesticides; D014874:Water Pollutants, Chemical; D000110:Acetylcholinesterase; D003976:Diazinon