[The study of apoptosis and mechanism of cells exposed to steep pulse].
This experiment was designed to study the apoptosis and related mechanism of adherent liver tumor cells (SMMC-7721) and adherent normal liver cells (HL-7702) when they were exposed to the steep pulse generated by the steep pulse apparatus for tumor treatment. The results showed that the steep pulse of 200 V could induce tumor cells apoptosis. The tumor cells presented with their apoptosis when they were exposed to the steep pulse from 200 V to 250 V. Laser scanning confocal microscopy was used to make a real time study of calcium burst when the adherent tumor cells were exposed to the steep pulse. The results showed:On the condition of no extracellular Ca2+, the concentration of Ca2+ in tumor cells exposed to the steep pulse of 150 V did not change; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 200 V decreased; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 250 V decreased more evidently. On the condition of existing extracellular Ca2+, the concentration of Ca2+ in tumor cells exposed to the steep pulse of 150 V did not change; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 200 V decreased little; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 250 V reduced little, too. Maybe the change of calcium burst in the tumor cells is the mechanism of apoptosis when cells are exposed to the steep pulse.
Liu
Huan
H
Bioengineering College of Chongqing University, Chongqing 400044, China.
Tang
Liling
L
Sun
Caixin
C
Mi
Yan
Y
Yao
Chenguo
C
Li
Chengxiang
C
Wang
Yuanliang
Y
D017209:Apoptosis / Q000528:radiation effects*; D002118:Calcium / Q000378:metabolism*; D004560:Electricity; D004574:Electromagnetic Fields; D022781:Hepatocytes / Q000166:cytology / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D018613:Microscopy, Confocal; D014407:Tumor Cells, Cultured
D002118:Calcium