Deproteinization of bacterial genomic and plasmid DNAs by tannic acid.
Tannic acid is proposed as a protein precipitating agent in genomic and plasmid DNAs preparation. After addition of tannic acid, a single centrifugation step is sufficient to deproteinize Escherichia coli and Serratia marcescens extracts. The purified DNA lent itself to amplification, restriction enzymes digestion and transformation. Tannic acid is much less toxic than common deproteinizing agents and it is environmentally friendly, biodegradable, renewable and quite inexpensive.
van Huynh
N
N
Department of Public Health, Veterinary Agrochem. Research Center (VAR), Virology Department, Groeselenberg 99, B 1180 Brussels, Belgium. loiusvanhuynh@gmail.com
D011232:Chemical Precipitation; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011506:Proteins / Q000737:chemistry*; D019105:Random Amplified Polymorphic DNA Technique; D012706:Serratia marcescens / Q000235:genetics; D013634:Tannins / Q000737:chemistry*
D004269:DNA, Bacterial; D011506:Proteins; D013634:Tannins