Intrafollicular antimüllerian hormone levels predict follicle responsiveness to follicle-stimulating hormone (FSH) in normoandrogenic ovulatory women undergoing gonadotropin releasing-hormone analog/recombinant human FSH therapy for in vitro fertilization and embryo transfer.
OBJECTIVE
To investigate the relationship between antimüllerian hormone (AMH) and steroidogenesis in follicles of normoandrogenic ovulatory women undergoing follicle-stimulating hormone (FSH) therapy for in vitro fertilization/embryo transfer (IVF-ET).


METHODS
Prospective cohort.


METHODS
Institutional/private practice.


METHODS
26 normoandrogenic ovulatory women. All women received gonadotropin-releasing hormone (GnRH) analog and ovarian stimulation for IVF-ET.


METHODS
Follicle fluid was aspirated at oocyte retrieval from the first follicle of each ovary.


METHODS
Follicle fluid was assayed for AMH, estradiol (E(2)), progesterone, androstenedione, testosterone, dihydrotestosterone, insulin, and FSH.


RESULTS
Intrafollicular AMH levels positively and negatively correlated with E(2) and FSH concentrations in follicles, respectively, causing a positive relationship between follicle fluid AMH levels and E(2)/FSH ratios as a measure of follicle sensitivity to FSH. A positive relationship also existed in follicles between AMH levels and E(2)/androgen ratios as a marker of aromatase activity.


CONCLUSIONS
The AMH levels in follicles of IVF patients positively correlate with follicle sensitivity to FSH.
Dumesic
Daniel A
DA
Reproductive Medicine and Infertility Associates, 2101 Woodwinds Drive, Woodbury, MN 55125, USA. danieldumesic@aol.com
Lesnick
Timothy G
TG
Stassart
Jacques P
JP
Ball
G David
GD
Wong
Ashley
A
Abbott
David H
DH
D000735:Androstenedione / Q000032:analysis; D054304:Anti-Mullerian Hormone / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000032:analysis; D004624:Embryo Transfer / Q000379:methods*; D004958:Estradiol / Q000032:analysis; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000032:analysis / Q000627:therapeutic use*; D015571:Follicular Fluid / Q000737:chemistry; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D006801:Humans; D006080:Ovarian Follicle / Q000502:physiology*; D010060:Ovulation / Q000502:physiology*; D011247:Pregnancy; D011374:Progesterone / Q000032:analysis; D011994:Recombinant Proteins / Q000627:therapeutic use; D013739:Testosterone / Q000032:analysis
D011994:Recombinant Proteins; D013196:Dihydrotestosterone; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone