Production of 10-hydroxy-8(E)-octadecenoic acid from oleic acid conversion by strains of Pseudomonas aeruginosa.
Eighteen Pseudomonas aeruginosa strains were examined for their ability to convert oleic acid to produce 10-hydroxy-8(E)-octadecenoic acid (HOD), which was structurally confirmed by GC-MS, NMR, and FTIR. There were no substantial amounts of other new compounds found in the fermentation broths in addition to HOD and 7,10-dihydroxy-8(E)-octadecenoic acid (DOD). The results demonstrated that P. aeruginosa strains possessed varying levels of activity for producing HOD. Under the experimental conditions, strain NRRL B-14938 isolated from sheep manure was the best HOD producer exhibiting the highest HOD to DOD product ratio in the medium most suitable for purifying HOD. Using strain B-14938 as a model system for further characterization, optimum conditions for producing HOD were found to be at 26 degrees C and pH 7.0 after 60 h of reaction time using a medium containing EDTA as a chelating agent. This study has identified a high-yielding P. aeruginosa strain and provided the reaction characteristics needed to develop a scale-up production process of HOD for testing its properties and potential new uses.
Kuo
Tsung Min
TM
Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, IL, 61604, USA.
Huang
Jenq-Kuen
JK
Labeda
David
D
Wen
Lisa
L
Knothe
Gerhard
G
D000818:Animals; D001711:Biotransformation; D005285:Fermentation; D007218:Industrial Microbiology; D008372:Manure / Q000382:microbiology*; D019301:Oleic Acid / Q000378:metabolism*; D009829:Oleic Acids / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000378:metabolism*; D012756:Sheep
C107324:10-hydroxy-8-octadecenoic acid; C477043:7,10-dihydroxy-8-octadecenoic acid; D008372:Manure; D009829:Oleic Acids; D019301:Oleic Acid