title: Surface-immobilised antimicrobial peptoids.
abstract: Surface modification techniques that create surfaces capable of killing adherent bacteria are promising solutions to infections associated with implantable medical devices. Antimicrobial (AM) peptoid oligomers (ampetoids) that were designed to mimic helical AM peptides were synthesised with a peptoid spacer chain to allow mobility and an adhesive peptide moiety for easy and robust immobilisation onto substrata. TiO(2) substrata were modified with the ampetoids and subsequently backfilled with an antifouling (AF) polypeptoid polymer in order to create polymer surface coatings composed of both AM (active) and AF (passive) peptoid functionalities. Confocal microscopy images showed that the membranes of adherent E. coli cells were damaged after 2-h exposure to the modified substrata, suggesting that ampetoids retain AM properties even when immobilised on substrata.
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
authors.forename: Andrea R
authors.initials: AR
authors.lastname: Statz
authors.forename: Jong Pil
authors.initials: JP
authors.lastname: Park
authors.forename: Nathaniel P
authors.initials: NP
authors.lastname: Chongsiriwatana
authors.forename: Annelise E
authors.initials: AE
authors.lastname: Barron
authors.forename: Phillip B
authors.initials: PB
authors.lastname: Messersmith
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002942:Circular Dichroism; D004926:Escherichia coli / Q000187:drug effects* / Q000648:ultrastructure; D051379:Mice; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D015394:Molecular Structure; D034444:Peptoids / Q000138:chemical synthesis* / Q000494:pharmacology*; D013499:Surface Properties; D041701:Swiss 3T3 Cells; D014025:Titanium / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D034444:Peptoids; C009495:titanium dioxide; D014025:Titanium