title: Gasdermin D (Gsdmd) is dispensable for mouse intestinal epithelium development.
abstract: Members of the novel gene family Gasdermin (Gsdm) are exclusively expressed in a highly tissue-specific manner in the epithelium of skin and the gastrointestinal tract. Based on their expression patterns and the phenotype of the Gsdma3 spontaneous mutations, it is inferred that the Gsdm family genes are involved in epithelial cell growth and/or differentiations in different tissues. To investigate possible roles of the Gsdm gene family in the development of intestinal tracts, we generated a Gsdmd mutant mouse, which is a solitary member of the Gsdmd subfamily and which is predominantly expressed in the intestinal tract by means of targeted disruption. In the mutant homozygotes, we found no abnormality of intestinal tract morphology. Moreover, in mutant mice, there was normal differentiation of all constituent cell types of the intestinal epithelium. Thus, this study clearly shows that Gsdmd is not essential for development of mouse intestinal tract or epithelial cell differentiation.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Fujii
authors.forename: Masaru
authors.initials: M
authors.lastname: Tamura
authors.forename: Shigekazu
authors.initials: S
authors.lastname: Tanaka
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Kato
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Youichi
authors.initials: Y
authors.lastname: Mizushina
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Shiroishi
mesh_terms: D000818:Animals; D007413:Intestinal Mucosa / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D038081:Organogenesis; D000094524:Gasdermins
chemical_list: D009363:Neoplasm Proteins; C413482:Gsdma protein, mouse; D000094524:Gasdermins