title: Differential involvement of GABA system in mediating behavioral and neurochemical effect of acupuncture in ethanol-withdrawn rats.
abstract: In our previous study we demonstrated that acupuncture at Shenmen (HT7) points suppressed a decrease of accumbal dopamine (DA) release in ethanol-withdrawn rats. Furthermore, here we found that it inhibited behavioral withdrawal signs of ethanol. In an effort to better understand the mechanisms underlying this inhibition, the potential role of GABA receptor system in acupuncture was investigated. Male Sprague-Dawley rats were treated with 3g/kg/day of ethanol (20%, w/v) or saline by intraperitoneal injection for 21 days. Following 48 or 72h of ethanol withdrawal, acupuncture was applied at bilateral HT7 for 1min. The selective GABA(A) antagonist bicuculline and the selective GABA(B) antagonist SCH 50911 were injected intraperitoneally 20min before acupuncture, respectively. Importantly, suppressive effects of acupuncture on DA deficiency were completely abolished by SCH 50911, but not by bicuculline, whereas ameliorating effects of acupuncture on ethanol withdrawal syndrome were completely blocked either by SCH 50911 or bicuculline. These results suggest that acupuncture at specific acupoint HT7 may normalize the DA release in the mesolimbic system and attenuate withdrawal syndrome through the GABA(B) receptor system in ethanol-withdrawn rats.
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu, South Korea.
authors.forename: Bong Hyo
authors.initials: BH
authors.lastname: Lee
authors.forename: Rong Jie
authors.initials: RJ
authors.lastname: Zhao
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Moon
authors.forename: Seong Shoon
authors.initials: SS
authors.lastname: Yoon
authors.forename: Jung-Ae
authors.initials: JA
authors.lastname: Kim
authors.forename: Heeduk
authors.initials: H
authors.lastname: An
authors.forename: Young Kyu
authors.initials: YK
authors.lastname: Kwon
authors.forename: Meeyul
authors.initials: M
authors.lastname: Hwang
authors.forename: Seong Hun
authors.initials: SH
authors.lastname: Choi
authors.forename: Insop
authors.initials: I
authors.lastname: Shim
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Kim
authors.forename: Chae Ha
authors.initials: CH
authors.lastname: Yang
mesh_terms: D026881:Acupuncture / Q000379:methods*; D015669:Acupuncture Points; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D001640:Bicuculline / Q000494:pharmacology; D001923:Brain Chemistry / Q000502:physiology*; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D000431:Ethanol / Q000009:adverse effects*; D018682:GABA Agents / Q000494:pharmacology; D008297:Male; D009025:Morpholines / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013375:Substance Withdrawal Syndrome / Q000209:etiology* / Q000534:rehabilitation*; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: C095804:(+)-(S)-5,5-dimethylmorpholinyl-2-acetic acid; D018682:GABA Agents; D009025:Morpholines; D000431:Ethanol; D005680:gamma-Aminobutyric Acid; D004298:Dopamine; D001640:Bicuculline