title: [Analysis of the allergenicity and immunogenicity of Psilogramma menephron allergen].
abstract: OBJECTIVE
  To analyze the allergenicity and immunogenicity of Psilogramma menephron allergen so as to provide the basis for preparing recombinant and standardized allergen vaccines of Psilgramma menephorn.
  
  
  METHODS
  The extracts of Psilgramma menephorn were analyzed by SDS-PAGE, and the allergenicity and immunogenicity of the extracts were tested with 9 sera from allergic patients by means of immunoblotting.
  
  
  RESULTS
  More than 20 allergen proteins were separated from the extract of Psilgramma menephorn by SDS-PAGE, with the relative molecular weight ranging from 12,000 to 128,000. The relative molecular weight of the allergenic proteins were 74,000 (88.9%), 66,000 (22.2%), 49,000 (22.2%), 36,000 (77.8%), or 25,000 (33.3%), and those of the immunogenic proteins were 79,000 (33.3%), 74,000 (66.7%), 66,000 (22.2%), 49,000 (22.2%), 36,000 (44.4%), or 25,000 (55.6%).
  
  
  CONCLUSIONS
  The relative molecular weight of the major allergenic proteins of Psilgramma menephorn are 74,000 and 36,000, and 74,000 and 25,000 for the major immunogenic proteins. These proteins constitute the major allergenic components for diagnosis and specific treatment of Psilgramma menephorn allergy.
authors.affiliation: Respiratory Department of the Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Shannxi, Xi'an 710004, China.
authors.forename: Xiu-zhen
authors.initials: XZ
authors.lastname: Sun
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Ding
authors.initials: D
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xiang-li
authors.initials: XL
authors.lastname: Feng
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D001249:Asthma / Q000097:blood / Q000276:immunology*; D015153:Blotting, Western; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007915:Lepidoptera / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000485:Allergens; D007074:Immunoglobulin G