title: Quality evaluation of umbilical cord blood progenitor cells cryopreserved with a small-scale automated liquid nitrogen system.
abstract: The performance of a small-scale automated cryopreservation and storage system (Mini-BioArchive system) used in the banking of umbilical cord blood (UCB) units was evaluated. After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34+ cells, colony-forming unit-granulocyte-macrophage (CFU-GM), and total CFU were analyzed. Twenty UCB units cryopreserved using the automated system and stored for a median of 34 days were analyzed. Mean CD34+ cell viabilities before freezing were 99.8+/-0.5% and after thawing were 99.8+/-0.4% in the large bag compartments and 99.7+/-0.5% in the small compartments. The mean recovery values for total nucleated cells, CD34+ cells, CFU-GM, and total CFU were 94.8+/-16.0%, 99.3+/-18.6%, 103.9+/-20.6%, and 94.3+/-12.5%, respectively in the large compartments, and 95.8+/-25.9%, 106.8+/-23.9%, 101.3+/-23.3%, and 93.8+/-19.2%, respectively in the small compartments. A small-scale automated cryopreservation and storage system did not impair the clonogenic capacity of UCB HPCs. This cryopreservation system could provide cellular products adequate for UCB banking and HPC transplantation.
authors.affiliation: Division of Blood Transfusion, Tohoku University Hospital, 1-1 Seiryomachi, Aoba-ku, Sendai, Miyagi Prefecture 980-8574, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Miura
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Minegishi
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Itoh
authors.forename: Tamie
authors.initials: T
authors.lastname: Kitaura
authors.forename: Narumi
authors.initials: N
authors.lastname: Fukawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Akira
authors.initials: A
authors.lastname: Suzuki
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kudo
authors.forename: Ayuko
authors.initials: A
authors.lastname: Narita
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sato
authors.forename: Masakuni
authors.initials: M
authors.lastname: Suzuki
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Wada
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Takeyama
authors.forename: Takanori
authors.initials: T
authors.lastname: Watanabe
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tsuchiya
mesh_terms: D018952:Antigens, CD34; D001771:Blood Banks; D001793:Blood Preservation; D002470:Cell Survival; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003114:Colony-Forming Units Assay; D015925:Cryopreservation / Q000295:instrumentation* / Q000379:methods; D003911:Dextrans; D004121:Dimethyl Sulfoxide; D005260:Female; D005312:Fetal Blood; D005434:Flow Cytometry; D005615:Freezing; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D009584:Nitrogen; D013997:Time Factors
chemical_list: D018952:Antigens, CD34; D003911:Dextrans; D009584:Nitrogen; D004121:Dimethyl Sulfoxide