Identification of copy number alterations and its association with pathological features in clear cell and papillary RCC.
We report and characterize the copy number alterations (CNAs) in 35 clear cell and 12 papillary renal cell carcinomas (RCC) using Affymetrix 100K SNP arrays. Novel gain and loss regions are identified in both subtypes. In addition, statistically significant CNA are detected and associated with the pathological features: VHL mutation status, tumor grades, and sarcomatoid component in clear cell RCC and in types 1 and 2 of papillary RCC. Florescence in situ hybridization confirmed the copy number gain in the transforming growth factor, beta-induced gene (TGFBI), which is a possible oncogene for clear cell RCC.
Matsuda
Daisuke
D
Laboratory of Cancer Genetics/Sequencing, Van Andel Research Institute, 333 Bostwick Avenue N.E., Grand Rapids, MI 49503, USA.
Khoo
Sok Kean
SK
Massie
Aaron
A
Iwamura
Masatsugu
M
Chen
Jindong
J
Petillo
David
D
Wondergem
Bill
B
Avallone
Michael
M
Kloostra
Stephanie J
SJ
Tan
Min-Han
MH
Koeman
Julie
J
Zhang
Zhongfa
Z
Kahnoski
Richard J
RJ
Baba
Shiro
S
Teh
Bin Tean
BT
D000368:Aged; D001483:Base Sequence; D002291:Carcinoma, Papillary / Q000235:genetics* / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D002877:Chromosomes, Human; D017931:DNA Primers; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D051794:Von Hippel-Lindau Tumor Suppressor Protein / Q000235:genetics
D017931:DNA Primers; D051794:Von Hippel-Lindau Tumor Suppressor Protein; C497446:VHL protein, human