title: Blood F2-isoprostanes are significantly associated with abnormalities of lipid status in rats with steatosis.
abstract: OBJECTIVE
  To investigate oxidative stress and lipid peroxidation in hepatic steatosis and the underlying implications in pathological mechanisms of non-alcoholic fatty liver disease (NAFLD).
  
  
  METHODS
  F(2)-isoprostanes (iPF(2alpha)-III) in blood and liver samples from steatotic (n = 9) and control (n = 7) rats were measured as in vivo marker of lipid peroxidation by a mass spectrometric approach. The lipid profile and endogenous antioxidant status (SOD and CAT) in the rats were also analyzed.
  
  
  RESULTS
  Significantly higher levels of iPF(2alpha)-III (mean 3.47 vs 2.40 pmol/mg tissue, P = 0.004) and lower activities of SOD (mean 1.26 U vs 1.40 U, P < 0.001) and CAT (mean 1026.36 U/mg vs 1149.68 U/mg protein, without significance) were observed in the livers of steatotic rats. Plasma total iPF(2alpha)-III was significantly correlated with the abnormalities of blood lipids as well as alanine aminotransferase (ALT) levels in the rats with simple steatosis, whereas no similar tendencies were observed in the control rats.
  
  
  CONCLUSIONS
  Enhancement of hepatic oxidative imbalance occurring at the steatotic stage of NAFLD suggests a possibility that manifestation of the local oxidative damage precedes that of systemic oxidative imbalance. Predominant metabolic features of the increased lipid peroxidation further suggest a close association of the oxidative imbalance and the dyslipidemia with functional deterioration of the steatotic liver. The findings need to be further evaluated, especially in human studies.
authors.affiliation: Shanghai Institute of Brain Functional Genomics, East China Normal University, 3663 Zhongshan North Road, Shanghai, China.
authors.forename: Man-Jie
authors.initials: MJ
authors.lastname: Zhu
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Sun
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Liu
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Feng
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Tong
authors.forename: Ying-He
authors.initials: YH
authors.lastname: Hu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001835:Body Weight / Q000502:physiology; D004195:Disease Models, Animal; D028441:F2-Isoprostanes / Q000097:blood*; D005234:Fatty Liver / Q000097:blood* / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000502:physiology; D008055:Lipids / Q000097:blood*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes; D008055:Lipids