Hematopoietic recovery after transplantation CD117+B220- (LACZ*) bone marrow cells in lethally irradiated mice.
Experiments presented here were aimed at the description of hematopoiesis repair and in vivo homing of transplanted separated CD117+B220- bone marrow cells after whole-body lethal irradiation at LD 9Gy. ROSA 26 mice were used as donors of marrow cells for transplantation [B6;129S/Gt (ROSA)26Sor] and were tagged with lacZ gene, and F2 hybrid mice [B6129SF2/J] were used as recipients of bone marrow transplanted cells. Hematopoiesis repair was provided by transplantation, both suspension of whole bone marrow cells (5x106) and isolated CD117+B220- cells (5x10(4)). Mice survived up to thirty days after irradiation. We demonstrated that transplantation of suspension of whole bone marrow cells led to faster recovery of CFU-GM (Granulocyte-macrophage colony forming units) in bone marrow and in the spleen too. It is not clear what the share of residential and transplanted cells is in the repair process. Our results demonstrate that sufficient hematopoietic repair occurs after transplantation of CD117+B220- (lacZ+) in lethally irradiated mice, and the difference in CFU-GM numbers in the bone marrow and spleen found on day 8 posttransplant has no influence on the survival of lethally irradiated mice (30 days follow-up).
Hodek
Miroslav
M
Department of Oncology and Radiotherapy, Charles University in Prague, Faculty of Medicine and University Hospital Hradec Kralove, Czech Republic. hodek.miroslav@email.cz
Vávrová
Jirina
J
Sinkorová
Zuzana
Z
Mokrý
Jaroslav
J
Filip
Stanislav
S
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000528:radiation effects*; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis / Q000528:radiation effects; D006411:Hematopoiesis, Extramedullary / Q000528:radiation effects; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D011829:Radiation Dosage; D013154:Spleen / Q000502:physiology / Q000528:radiation effects
D019009:Proto-Oncogene Proteins c-kit; D017493:Leukocyte Common Antigens