title: Comparative studies on the aggregation behavior of HBPs from human seminal plasma by dynamic light scattering.
abstract: Heparin binding proteins (HBPs) from human seminal plasma (HSP) were obtained by heparin affinity and size exclusion chromatography. The aggregation/disaggregation of HBPs was followed by dynamic light scattering (DLS) in presence of various physiological ligands such as CaCl(2), NaCl, EDTA, cholesterol, adenosine, D-glucose and D-fructose. The aggregation pattern of lactoferrin was also analyzed and compared. The study of interactions of HBPs of HSP may contribute to an understanding mechanisms underlying the fertilization process.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
authors.forename: Md Imtaiyaz
authors.initials: MI
authors.lastname: Hassan
authors.forename: Tej P
authors.initials: TP
authors.lastname: Singh
authors.forename: Savita
authors.initials: S
authors.lastname: Yadav
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D002784:Cholesterol / Q000494:pharmacology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005632:Fructose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006493:Heparin / Q000378:metabolism; D006801:Humans; D008027:Light; D008297:Male; D011485:Protein Binding / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D012542:Scattering, Radiation; D029589:Seminal Plasma Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D029589:Seminal Plasma Proteins; D005632:Fructose; D006493:Heparin; D002784:Cholesterol; D005947:Glucose; D000241:Adenosine