title: Subcellular curvature at the perimeter of micropatterned cells influences lamellipodial distribution and cell polarity.
abstract: This paper employs substrates that are patterned with shapes having well-defined geometric cues to characterize the influence of curvature on the polarization of highly metastatic B16F10 rat melanoma cells. Substrates were patterned using microcontact printing to define adhesive islands of defined shape and size on a background that otherwise prevents cell adhesion. Cells adherent to these surfaces responded to local curvature at the perimeter of the adhesive islands; convex features promoted the assembly of lamellipodia and concave features promoted the assembly of stress filaments. Cells adherent to rectangular shapes displayed a polarized cytoskeleton that increased with the aspect ratio of the shapes. Shapes that combined local geometric cues, by way of concave or convex edges, with aspect ratio were used to understand the additive effects of shape on polarization. The dependence of cell polarity on shape was determined in the presence of small molecules that alter actomyosin contractility and revealed a stronger dependence on contractility for shapes having straight edges, in contrast to those having curved edges. This study demonstrates that the cytoskeleton modulates cell polarity in response to multiple geometric cues in the extracellular environment.
authors.affiliation: Department of Cell and Molecular Biology, Northwestern University, Chicago, Illinois, USA.
authors.forename: Jane
authors.initials: J
authors.lastname: James
authors.forename: Edgar D
authors.initials: ED
authors.lastname: Goluch
authors.forename: Huan
authors.initials: H
authors.lastname: Hu
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Milan
authors.initials: M
authors.lastname: Mrksich
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D016764:Cell Polarity; D048430:Cell Shape; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D009362:Neoplasm Metastasis; D011554:Pseudopodia / Q000378:metabolism*; D051381:Rats; D022502:Stress Fibers / Q000378:metabolism
chemical_list: D000199:Actins