Simultaneous directed assembly of three distinct heterodimeric coiled coils.
We describe simultaneous formation of three distinct heterodimeric coiled coils from a mixture of six different peptides. The choice among electrostatically viable complexes is governed by alignment of buried core residues, including a fundamentally new interaction that exploits urea-terminated side chains. Buried urea/urea contacts lead to extremely stable dimeric coiled coils, with T(m) values between 63 and 79 degrees C. Core ureas can also form stable complexes with a variety of other polar groups, including guanidines, acids, and amides.
Diss
Maria L
ML
Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, USA.
Kennan
Alan J
AJ
D000595:Amino Acid Sequence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D017433:Protein Structure, Secondary; D014508:Urea / Q000031:analogs & derivatives* / Q000737:chemistry
D010455:Peptides; D014508:Urea