[Establishment of simultaneous measurement method of 8 salivary components using urinary test paper and clinical evaluation of oral environment].
OBJECTIVE
Clinical findings were compared with glucose, protein, albumin, bilirubin, creatinine, pH, occult blood, ketone body, nitrite, and white blood cells contained in whole saliva to investigate the components that most markedly reflect the periodontal condition.


METHODS
The subjects were staff of the Prosthodontics Department, Showa University, and patients who visited for dental treatments (57 subjects in total). At the first time, saliva samples were gargled with 1.5 ml of distilled water for 15 seconds and collected by spitting out into a paper cup. At the second time, saliva samples were collected by the same method. At the third time, saliva samples after chewing paraffin gum for 60 seconds were collected by spitting out into a paper cup. Thus whole saliva collecting that was divided on three times. After sampling, 8 mul of the saliva sample was dripped in reagent sticks for the 10 items of urinary test paper and the reflectance was measured using a specific reflectometer. In the periodontal tissue evaluation, the degree of alveolar bone resorption, probing value, and tooth mobility and the presence or absence of lesions in the root furcation were examined and classified into 4 ranks. The mean values in each periodontal disease rank and correlation between the periodontal disease ranks and the components were statistically analyzed.


RESULTS
Bilirubin and ketone body were not measurable. The components density of the 8 items was increased as the periodontal disease rank increased. Regarding the correlation between the periodontal disease ranks and the components, high correlations were noted for protein, albumin, creatinine, pH, and white blood cells.


CONCLUSIONS
The simultaneous measurement method of 8 salivary components using test paper may be very useful for the diagnosis of periodontal disease of abutment teeth.
Yuuki
Kenji
K
Department of Prosthodontics, Showa University School of Dentistry. tibinori32000@yahoo.co.jp
Tsukasaki
Hiroaki
H
Kawawa
Tadaharu
T
Shiba
Akihiko
A
Shiba
Kiyoko
K
D000328:Adult; D000368:Aged; D000418:Albumins / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D003404:Creatinine / Q000032:analysis; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D010510:Periodontal Diseases / Q000175:diagnosis*; D011506:Proteins / Q000032:analysis*; D012463:Saliva / Q000737:chemistry* / Q000166:cytology; D016482:Urinalysis / Q000379:methods*
D000418:Albumins; D015415:Biomarkers; D011506:Proteins; D003404:Creatinine