title: Cloning and functional expression of an alpha-galactosidase from Yersinia pestis biovar Microtus str. 91001.
abstract: A gene encoding a glycoside hydrolase (GH) family 36 alpha-galactosidase was cloned from Yersinia pestis biovar Microtus str. 91001 and expressed in Escherichia coli. The purified recombinant alpha-galactosidase (Aga-Y) was optimally active at 37 degrees C and pH 6.8. The features of temperature profile, thermoliability, kinetics, and amino acid composition indicated that Aga-Y had properties of a cold-adapted enzyme.
authors.affiliation: Department of Microbial Engineering, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Cao
authors.forename: Huoqing
authors.initials: H
authors.lastname: Huang
authors.forename: Kun
authors.initials: K
authors.lastname: Meng
authors.forename: Peilong
authors.initials: P
authors.lastname: Yang
authors.forename: Pengjun
authors.initials: P
authors.lastname: Shi
authors.forename: Yaru
authors.initials: Y
authors.lastname: Wang
authors.forename: Huiying
authors.initials: H
authors.lastname: Luo
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ningfeng
authors.initials: N
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
mesh_terms: D003001:Cloning, Molecular; D004795:Enzyme Stability; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D015010:Yersinia pestis / Q000201:enzymology* / Q000235:genetics; D000519:alpha-Galactosidase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D000519:alpha-Galactosidase