title: Diverse hematopoietic potentials of five human embryonic stem cell lines.
abstract: Despite a growing body of literature concerning the hematopoietic differentiation of human embryonic stem cells (hESCs), the full hematopoietic potential of the majority of existing hESC lines remains unknown. In this study, the hematopoietic response of five NIH-approved hESC lines (H1, hSF6, BG01, BG02, and BG03) was compared. Our data show that despite expressing similar hESC markers under self-renewing conditions and initiating mesodermal differentiation under spontaneous differentiation conditions, marked differences in subsequent hematopoietic differentiation potential among these lines existed. A high degree of hematopoietic differentiation was attained only by H1 and BG02, whereas this process appeared to be abortive in nature for hSF6, BG01, and BG03. This difference in hematopoietic differentiation predisposition was readily apparent during spontaneous differentiation, and further augmented under hematopoietic-inducing conditions. This predisposition appeared to be intrinsic to the specific hESC line and independent of passage number or gender karyotype. Interestingly, H1 and BG02 displayed remarkable similarities in their kinetics of hematopoietic marker expression, hematopoietic colony formation, erythroid differentiation, and globin expression, suggesting that a similar, predetermined differentiation sequence is followed. The identification of intrinsic and extrinsic factors governing the hematopoietic differentiation potential of hESCs will be of great importance for the putative clinical utility of hESC lines.
authors.affiliation: Department of Medicine, Hematology Division, University of Washington, 1705 NE Pacific Street, Box 357710, Seattle, WA, USA.
authors.forename: Kai-Hsin
authors.initials: KH
authors.lastname: Chang
authors.forename: Angelique M
authors.initials: AM
authors.lastname: Nelson
authors.forename: Paul A
authors.initials: PA
authors.lastname: Fields
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Hesson
authors.forename: Tatiana
authors.initials: T
authors.lastname: Ulyanova
authors.forename: Hua
authors.initials: H
authors.lastname: Cao
authors.forename: Betty
authors.initials: B
authors.lastname: Nakamoto
authors.forename: Carol B
authors.initials: CB
authors.lastname: Ware
authors.forename: Thalia
authors.initials: T
authors.lastname: Papayannopoulou
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D019070:Cell Lineage; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D050982:GATA1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D051379:Mice; D010641:Phenotype
chemical_list: D015415:Biomarkers; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human