Vimentin is required for dengue virus serotype 2 infection but microtubules are not necessary for this process.
The present study investigated the effect of microtubules (MTs) and vimentin during dengue virus serotype 2 (DV2) infection. Immunostaining showed that DV2 infection induced MT and vimentin reorganization. Colocalization of DV2 antigens with MTs or vimentin were often observed in ECV304 cells. MT-disrupting agents could enhance DV2 release but did not affect other steps of virus replication. In contrast, disruption of vimentin inhibited DV2 infection. Our results suggest that an MT-dependent mechanism may not be necessary for DV2 infection, and MT disruption may promote DV2 release. However, vimentin is required for DV2 infection.
Chen
Wei
W
Department of Microbiology, Third Military Medical University, 400038 Chongqing, People's Republic of China.
Gao
Na
N
Wang
Jia-li
JL
Tian
Yan-ping
YP
Chen
Zong-tao
ZT
An
Jing
J
D002460:Cell Line; D003715:Dengue / Q000378:metabolism* / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D006801:Humans; D008870:Microtubules / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D014746:Vimentin / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D014746:Vimentin