ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome.
The small serine resolvase ParA from bacterial plasmids RK2 and RP4 catalyzes the recombination of two identical 133 bp recombination sites known as MRS. Previously, we reported that ParA is active in the fission yeast Schizosaccharomyces pombe. In this work, the parA recombinase gene was placed under the control of the Arabidopsis OXS3 promoter and introduced into Arabidopsis lines harboring a chromosomally integrated MRS-flanked target. The ParA recombinase excised the MRS-flanked DNA and the excision event was detected in subsequent generations in the absence of ParA, indicating germinal transmission of the excision event. The precise site-specific deletion by the ParA recombination system in planta demonstrates that the ParA recombinase can be used to remove transgenic DNA, such as selectable markers or other introduced transgenes that are no longer desired in the final product.
Thomson
James G
JG
Plant Gene Expression Center, 800 Buchanan Street, Albany, CA 94710, USA. jthomson@pw.usda.gov
Yau
Yuan-Yeu
YY
Blanvillain
Robert
R
Nunes
Wylla M
WM
Chiniquy
Dawn
D
Thilmony
Roger
R
Ow
David W
DW
D017360:Arabidopsis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D032461:Chromosomes, Plant / Q000235:genetics; D004247:DNA / Q000235:genetics*; D005821:Genetic Techniques; D018745:Genome, Plant; D008957:Models, Genetic; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D045522:Recombinases / Q000235:genetics*; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000378:metabolism
D001426:Bacterial Proteins; D045522:Recombinases; C052883:chromosome partition proteins, bacterial; D004247:DNA