title: Nocardioides fonticola sp. nov., a novel actinomycete isolated from spring water.
abstract: A Gram-positive, rod-shaped, yellow-pigmented actinomycete, designated strain NAA-13T, was isolated from a freshwater spring located in Kaoshiung, southern Taiwan. This strain was characterized using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Nocardioides. The strain contained LL-diaminopimelic acid as the principal diamino acid of the peptidoglycan, MK-8(H4) as the major menaquinone, and iso-C16:0 and C17:0 as the predominant fatty acids. 16S rRNA gene sequence studies showed that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Nocardioides; its closest neighbours were Nocardioides insulae DS-51T (94.9 % similarity), Nocardioides pyridinolyticus KCTC 0074BP T (96.5 % similarity) and Nocardioides aquiterrae GW-9T (96.6 % similarity). The DNA G+C content of strain NAA-13T was 71.8 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain NAA-13T represents a novel species of the genus Nocardioides, for which the name Nocardioides fonticola sp. nov. is proposed. The type strain is NAA-13T (=LMG 24213T=BCRC 16874T).
authors.affiliation: Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Jui-Hsing
authors.initials: JH
authors.lastname: Chou
authors.forename: Nian-Tsz
authors.initials: NT
authors.lastname: Cho
authors.forename: A B
authors.initials: AB
authors.lastname: Arun
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013624:Taiwan
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S