Simultaneous determination of five main active constituents of Erigeron multiradiatus by HPLC-DAD-MS.
An HPLC-DAD-MS method was developed for simultaneous determination of the five major active constituents in Erigeron multiradiatus (Wall.) Benth, namely 6'-O-cafferylerigeroside (1), scutellarin (2), apigenin-7-O-beta-d-glucuronide (3), apigenin (4) and kaempferol (5), respectively. They were identified by ESI-MS and comparisons with literature. A comprehensive validation of the method included tests of sensitivity, linearity, precision and accuracy. The linear regressions were acquired with r>0.999. The precision was evaluated by intra- and inter-day assays, and relative standard deviation (R.S.D.) values were reported within 2.7%. The recovery studies for the quantified compounds were observed in the range of 95.3-102.4% with R.S.D. values less than 2.3%. The overall procedure may be suitable for the qualitative and quantitative analyses of a large number of E. multiradiatus samples. Hierarchical clustering analysis based on the characteristics of the 5 investigated compound peaks in HPLC profiles showed that 18 samples were divided into 2 main clusters. The clusters corresponded to their content. The five constituents in E. multiradiatus are generally regarded as an index for the quality assessment of this herb.
Zhang
Zhifeng
Z
West China School of Pharmacy, Sichuan University, Sichuan 610041, PR China.
Sun
Wenxia
W
Luo
Pei
P
Wu
Liping
L
Ye
Limin
L
Zhang
Hao
H
D047310:Apigenin / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D049919:Erigeron / Q000737:chemistry*; D005965:Glucuronates / Q000032:analysis* / Q000737:chemistry; D006358:Hot Temperature; D044949:Kaempferols / Q000032:analysis* / Q000737:chemistry; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D011753:Pyrones / Q000032:analysis* / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013997:Time Factors
C535256:6'-O-cafferylerigeroside; D005965:Glucuronates; D044949:Kaempferols; D010936:Plant Extracts; D011753:Pyrones; C456097:apigenin-7-O-glucuronide; C484876:scutellarin; C030416:erigeroside; C006552:kaempferol; D047310:Apigenin