Acyclovir susceptibility of herpes simplex virus isolates at King Chulalongkorn Memorial Hospital, Bangkok.
OBJECTIVE
To determine the ACV susceptibility in Thai HSV clinical isolates.


METHODS
One hundred thirty HSV isolates from the Virology Laboratory Unit, King Chulalongkorn Memorial Hospital, Bangkok Thailand had typing done by immunofluoresent assay using monoclonal antibody specific to either HSV-1 or HSV-2. Their sensitivity to ACV (IC50) was determined by plaque reduction assay.


RESULTS
The IC50 of 77 HSV-1 isolates ranged from 0.07-0.97 microg/ml and that of 53 HSV-2 isolates was 0.13-1.66 microg/ml. The standard HSV-1 (KOS) and HSV-2 (Baylor 186) were included in each run. The mean + standard deviation (SD) of ACV IC50 among HSV-1 and HSV-2 isolates were 0.38 +/- 0.23 and 0.50 +/- 0.32 microg/ml while that of standard HSV-1 and HSV-2 were 0.45 +/- 0.13 and 0.57 +/- 0.04 microg/ml. Statistically significant difference between IC50 of HSV-1 and HSV-2 isolates was indicated (p = 0.02).


CONCLUSIONS
No ACV(r) HSV has been detected and ACV susceptibility of HSV-2 has more resistance than that of HSV-1.
Sangdara
Ajchariyarat
A
Inter-department of Medical Microbiology, Faculty ofGraduate School, Chulalongkorn University, Bangkok, Thailand.
Bhattarakosol
Parvapan
P
D000212:Acyclovir / Q000627:therapeutic use*; D000998:Antiviral Agents / Q000627:therapeutic use*; D004198:Disease Susceptibility; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D010865:Pilot Projects; D012680:Sensitivity and Specificity; D013785:Thailand
D000998:Antiviral Agents; D000212:Acyclovir