title: Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B.
abstract: Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.
authors.affiliation: Department of Medical Information Technology, Fujita Health University College, Toyoake, Aichi, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ihira
authors.forename: Akane
authors.initials: A
authors.lastname: Ohta
authors.forename: Ken
authors.initials: K
authors.lastname: Sugata
authors.forename: Sadao
authors.initials: S
authors.lastname: Suga
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Asano
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005838:Genotype; D015654:Herpesvirus 6, Human / Q000145:classification* / Q000302:isolation & purification*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; C067488:GTMKAC-specific type II deoxyribonucleases