Effect of eccentric exercise-induced muscle damage on the dynamics of muscle oxygenation and pulmonary oxygen uptake.
Unaccustomed eccentric exercise has a profound impact on muscle structure and function. However, it is not known whether associated microvascular dysfunction disrupts the matching of O2 delivery (Qo2) to O2 utilization (Vo2). Near-infrared spectroscopy (NIRS) was used to test the hypothesis that eccentric exercise-induced muscle damage would elevate the muscle Qo2:Vo2 ratio during severe-intensity exercise while preserving the speed of the Vo2 kinetics at exercise onset. Nine physically active men completed "step" tests to severe-intensity exercise from an unloaded baseline on a cycle ergometer before (Pre) and 48 h after (Post) eccentric exercise (100 squats with a load corresponding to 70% of body mass). NIRS and breath-by-breath pulmonary Vo2 were measured continuously during the exercise tests and subsequently modeled using standard nonlinear regression techniques. There were no changes in phase II pulmonary Vo2 kinetics following the onset of exercise (time constant: Pre, 25 +/- 4 s; Post, 24 +/- 2 s; amplitude: Pre, 2.36 +/- 0.23 l/min; Post, 2.37 +/- 0.23 l/min; all P > 0.05). However, the primary (Pre, 14 +/- 3 s; Post, 19 +/- 3 s) and overall (Pre, 16 +/- 4 s; Post, 21 +/- 4 s) mean response time of the [HHb] response was significantly slower following eccentric exercise (P < 0.05). The slower [HHb] kinetics observed following eccentric exercise is consistent with an increased Qo2:Vo2 ratio during transitions to severe-intensity exercise. We propose that unchanged primary phase Vo2 kinetics are associated with an elevated Qo2:Vo2 ratio that preserves blood-myocyte O2 flux.
Davies
Rosemary C
RC
School of Sport and Health Sciences, University of Exeter, St. Luke's Campus, Heavitree Rd., Exeter EX1 2LU, United Kingdom. rd217@exeter.ac.uk
Eston
Roger G
RG
Poole
David C
DC
Rowlands
Ann V
AV
DiMenna
Fred
F
Wilkerson
Daryl P
DP
Twist
Craig
C
Jones
Andrew M
AM
D000222:Adaptation, Physiological; D000328:Adult; D015415:Biomarkers / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D015444:Exercise; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D008833:Microcirculation; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D009135:Muscular Diseases / Q000378:metabolism* / Q000473:pathology; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption; D011652:Pulmonary Circulation; D019265:Spectroscopy, Near-Infrared; D055815:Young Adult
D015415:Biomarkers; D006454:Hemoglobins; C100003:deoxyhemoglobin; D003402:Creatine Kinase; D010100:Oxygen