title: Variation of gene silencing involving endogenous microRNA in mammalian cells.
abstract: MicroRNAs (miRNAs) are small noncoding RNA and play a role in gene expression regulation by inhibiting translation of their target messenger RNAs (mRNAs). In this study, we investigated the effects of endogenous let-7 miRNA on the expression of target genes in various mammalian cells by means of two types of reporter plasmids possessing target sequences for let-7: one carries perfectly matched target sequence for let-7 in the 3'-untranslated region of the luciferase reporter gene to monitor RNA interference (RNAi) activity and the other has three bulged binding sites for let-7 to monitor translation-inhibition activity. The results indicate that different cells have different levels of gene silencing against the target reporter genes. The data presented here suggest that not only microRNA level but also target transcript level likely participate in the generation of a variety of gene silencing.
authors.affiliation: National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Tamura
authors.forename: Mariko
authors.initials: M
authors.lastname: Yoshida
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Hohjoh
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D020868:Gene Silencing; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D014162:Transfection
chemical_list: D035683:MicroRNAs; C487931:mirnlet7 microRNA, human