title: An extracellular galactoxylomannan of acapsular Cryptococcus laurentii mutant.
abstract: An extracellular galactoxylomannan (GalXM) composed of D-Gal (34.0%), D-Xyl (26.6%) and D-Man (31.0%), and a small amount of L-Ara (4.0%) and D-Glc (4.4%) has been isolated from culture medium of acapsulated mutant of Cryptococcus laurentii by ethanol precipitation and gel filtration. Phosphorylated polymer of Mw approximately 75,000 contained 90% carbohydrates, 3.9% phosphorus and 5.3% proteins. Results of chemical and spectroscopic studies showed a highly branched structure of GalXM with a 1,6-linked mannopyranosyl-galactopyranosyl backbone (approximately 44%) branched predominantly at C-2 and C-3 of mannosyl, and C-3 of galactosyl residues by side chains terminated mainly by xylosyl and mannosyl residues, and to a less extent by arabinosyl and glucosyl ones.
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, SK-845 38 Bratislava, Slovakia. chemcape@savba.sk
authors.forename: P
authors.initials: P
authors.lastname: Capek
authors.forename: J
authors.initials: J
authors.lastname: Dudíková
authors.forename: M
authors.initials: M
authors.lastname: Matulová
authors.forename: N
authors.initials: N
authors.lastname: Kolarova
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003454:Cryptococcus / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation / Q000235:genetics*; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C043689:galactoxylomannan