Growth inhibition in Chinese cabbage (Brassica rapa var. chinensis) growth exposed to di-n-butyl phthalate.
The toxicity and effects of di-n-butyl phthalate (DBP), an endocrine disruptor, on the growth of Chinese cabbage (Brassica rapa var. chinensis) were studied. Etiolation occurred on leaves of Chinese cabbage plant treated with 50mg/L of DBP for 42 d. DBP even below 1mg/L had a significant effect on the concentration of chlorophyll in Chinese cabbage and the biomass showed a severe decrease under treatment with more than 30 mg/L of DBP. At a concentration below 1mg/L of DBP, no significant difference in accumulation was found, but treatments with concentration exceeding 10, 30, 50 and 100mg/L all resulted in significant accumulation of DBP. Six protein spots extracted from leaf tissue of DBP-treated Chinese cabbage displaying a differential expression are shown in 2-DE maps. According to proteome level studies, three protein spots were found to increase and were identified, respectively, as acyl-[acyl-carrier-protein] desaturase (acyl-ACP desaturase), root phototropism protein 3 (RPT3) and ferredoxin-nitrite reductase (Fd-NiR). The other three protein spots were found to decrease and were identified respectively as dihydroflavonol-4-reductase (DFR), aminoacyl-tRNA synthetase (aaRS) and ATP synthase subunit beta. The key finding is that the other closely related plant, Bok choy (Brassica rapa subsp. chinensis), the subspecies of Chinese cabbage, respond differently to the same chemicals.
Liao
Chien-Sen
CS
Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan.
Yen
Jui-Hung
JH
Wang
Yei-Shung
YS
D029741:Brassica rapa / Q000187:drug effects / Q000254:growth & development*; D003993:Dibutyl Phthalate / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000494:pharmacology; D004798:Enzymes / Q000032:analysis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects; D010940:Plant Proteins / Q000032:analysis / Q000187:drug effects; D010968:Plasticizers / Q000494:pharmacology; D040901:Proteomics
D052244:Endocrine Disruptors; D004798:Enzymes; D010940:Plant Proteins; D010968:Plasticizers; D003993:Dibutyl Phthalate