title: CP110 suppresses primary cilia formation through its interaction with CEP290, a protein deficient in human ciliary disease.
abstract: Primary cilia are nonmotile organelles implicated in signaling and sensory functions. Understanding how primary cilia assemble could shed light on the many human diseases caused by mutations in ciliary proteins. The centrosomal protein CP110 is known to suppress ciliogenesis through an unknown mechanism. Here, we report that CP110 interacts with CEP290--a protein whose deficiency is implicated in human ciliary disease--in a discrete complex separable from other CP110 complexes involved in regulating the centrosome cycle. Ablation of CEP290 prevents ciliogenesis without affecting centrosome function or cell-cycle progression. Interaction with CEP290 is absolutely required for the ability of CP110 to suppress primary cilia formation. Furthermore, CEP290 and CP110 interact with Rab8a, a small GTPase required for cilia assembly. Depletion of CEP290 interferes with localization of Rab8a to centrosomes and cilia. Our results suggest that CEP290 cooperates with Rab8a to promote ciliogenesis and that this function is antagonized by CP110.
authors.affiliation: Department of Pathology and Cancer Institute, New York University School of Medicine, 522 1(st) Avenue, New York, NY 10016, USA.
authors.forename: William Y
authors.initials: WY
authors.lastname: Tsang
authors.forename: Carine
authors.initials: C
authors.lastname: Bossard
authors.forename: Hemant
authors.initials: H
authors.lastname: Khanna
authors.forename: Johan
authors.initials: J
authors.lastname: Peränen
authors.forename: Anand
authors.initials: A
authors.lastname: Swaroop
authors.forename: Vivek
authors.initials: V
authors.lastname: Malhotra
authors.forename: Brian David
authors.initials: BD
authors.lastname: Dynlacht
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D018385:Centrosome / Q000378:metabolism; D002923:Cilia / Q000378:metabolism* / Q000473:pathology*; D003598:Cytoskeletal Proteins; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008954:Models, Biological; D050505:Mutant Proteins / Q000378:metabolism; D009363:Neoplasm Proteins / Q000172:deficiency* / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D034622:RNA Interference; D020691:rab GTP-Binding Proteins / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; C467559:CCP110 protein, human; D018797:Cell Cycle Proteins; C493903:Cep290 protein, human; D003598:Cytoskeletal Proteins; D008869:Microtubule-Associated Proteins; D050505:Mutant Proteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D010750:Phosphoproteins; C082377:RAB8A protein, human; D020691:rab GTP-Binding Proteins