title: Simultaneous determination of p-arsanilic acid and roxarsone in feed by liquid chromatography-hydride generation online coupled with atomic fluorescence spectrometry.
abstract: A novel, simple and sensitive liquid chromatography-hydride generation online coupled with atomic fluorescence spectrometry (LC-HG-AFS) method was developed for simultaneous determination of p-arsanilic acid (p-ASA) and roxarsone in feed. 20% Methanol aqueous was used as extraction reagent, after preprocessing samples by ultrasonic oscillation, then injected into the chromatography Waters symmetry shield RP18 analytical column (150mm x 4.6mm, 5 microm), finally detected by an atomic fluorescence spectrometer. The calibration curves of analyses were linear over a range of concentrations (0.2-4mg L-1 and the correlation coefficients were higher than 0.9990. The limits of detection were 0.2 mg L-1. The method has been validated by linearity, precision and recovery. p-ASA and roxarsone in feed can be successfully and simultaneously determined using the developed method without a tedious pretreatment procedure.
authors.affiliation: Institute of Quality Standards and Testing Technology of Agri-products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Jianjing
authors.initials: J
authors.lastname: Liu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
authors.forename: Haibin
authors.initials: H
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.forename: Fengmei
authors.initials: F
authors.lastname: Sun
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Shuming
authors.initials: S
authors.lastname: Yang
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D001147:Arsanilic Acid / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D012406:Roxarsone / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods*
chemical_list: D012406:Roxarsone; D001147:Arsanilic Acid