[Changes of cytokines in mice immunized with recombinant Bb-EmII/3-Em14-3-3 vaccine of Echinococcus multilocularis].
OBJECTIVE
To study the effects of recombinant Bb-EmII/3-Em14-3-3 vaccine on cytokine secretion in mice challenged with protoscoleces of Echinococcus multilocularis.


METHODS
BALB/c mice were immunized with rBb-EmII/3-Em14-3-3 vaccine by subcutaneous injection, intramuscular injection, nasal mucosa inoculation or oral administration. After 12 weeks of immunization,all the mice were challenged with 50 protoscoleces of Echinococcus multilocularis by intraperitoneal injection. 18 weeks later, the mice were sacrificed and the splenocytes were stimulated with EmAg and ConA or LPS in vitro, then IL-12, IL-10, IFN-gamma and TNF-alpha in the culture supernatant were measured by ELISA.


RESULTS
The level of IFN-gamma, IL-12 and TNF-alpha were greatly higher than that of PBS control group (P<0.05 or P<0.01), and the level of IL-10 was lower. The level of IFN-gamma, IL-12, TNF-alpha and IL-10 in each group with EmAg and ConA or LPS stimulation was greatly higher than that in the group without stimulation(P<0.05 or P<0.01).


CONCLUSIONS
Th1 response is induced in mice challenged with Echinococcus multilocularis by rBb-EmII/3-Em14-3-3 vaccine, and the vaccine may enhance protective response against the challenge with protoscoleces of Echinococcus multilocularis.
Yang
Mei
M
Institute of Infectious and Parasitic Diseases, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. yangmei16@sina.com
Li
Wen-Gui
WG
D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D048210:Echinococcus multilocularis / Q000276:immunology* / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014611:Vaccination / Q000379:methods; D014612:Vaccines / Q000276:immunology*
D016207:Cytokines; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D014612:Vaccines; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma