Chromofluorescent indicator for intracellular Zn2+/Hg2+ dynamic exchange.
The fluorescence of NABQ increases remarkably in the presence of Zn(2+) and is quenched by Hg(2+). As shown by confocal imaging, NABQ-Zn(2+) can penetrate cells, where the bound Zn(2+) is exchanged for Hg(2+). This results in the concomitant export of Hg(2+) from the cells, showing that NABQ can act as a Zn(2+) carrier and as a Hg(2+) extracting agent in living cells.
Lee
Jeong Won
JW
Department of Chemistry, Korea University, Seoul 136-701, Korea.
Jung
Hyo Sung
HS
Kwon
Pil Seung
PS
Kim
Jong Wan
JW
Bartsch
Richard A
RA
Kim
Youngmee
Y
Kim
Sung-Jin
SJ
Kim
Jong Seung
JS
D000818:Animals; D002413:Cations, Divalent / Q000737:chemistry; D002460:Cell Line; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008628:Mercury / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D011798:Quinaldines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013449:Sulfonamides / Q000138:chemical synthesis / Q000737:chemistry; D015032:Zinc / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D000096926:Benzenesulfonamides
D002413:Cations, Divalent; D005456:Fluorescent Dyes; D008628:Mercury; D011798:Quinaldines; D013449:Sulfonamides; D015032:Zinc; D000096926:Benzenesulfonamides