title: TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription.
abstract: The RNA polymerase II core promoter is a structurally and functionally diverse transcriptional module. RNAi depletion and overexpression experiments revealed a genetic circuit that controls the balance of transcription from two core promoter motifs, the TATA box and the downstream core promoter element (DPE). In this circuit, TBP activates TATA-dependent transcription and represses DPE-dependent transcription, whereas Mot1 and NC2 block TBP function and thus repress TATA-dependent transcription and activate DPE-dependent transcription. This regulatory circuit is likely to be one means by which biological networks can transmit transcriptional signals, such as those from DPE-specific and TATA-specific enhancers, via distinct pathways.
authors.affiliation: Section of Molecular Biology, University of California at San Diego, La Jolla, California 92093, USA.
authors.forename: Jer-Yuan
authors.initials: JY
authors.lastname: Hsu
authors.forename: Tamar
authors.initials: T
authors.lastname: Juven-Gershon
authors.forename: Michael T
authors.initials: MT
authors.lastname: Marr
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Wright
authors.forename: Robert
authors.initials: R
authors.lastname: Tjian
authors.forename: James T
authors.initials: JT
authors.lastname: Kadonaga
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics; D016385:TATA Box; D035181:TATA-Box Binding Protein / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D029721:Drosophila Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; D012319:RNA Polymerase II