Denitration of L-type calcium channel.
Tyrosine nitration results in altered function of selective proteins, including human smooth muscle L-type calcium channel, hCa(v)1.2b. We report here that Ca(v)1.2 is also subject to "denitration". Cell lysates from activated macrophage-like cell line, RAW264.7 cells, reversed peroxynitrite-induced nitration of the carboxy terminus of Ca(v)1.2 in a 1D gel assay. Tyrosine phosphorylation of the calcium channel by c-src kinase was blocked by nitration but reversed by pretreatment with RAW264.7 cell lysates. These findings indicate that denitration may be a physiological mechanism to restore cellular excitability during inflammation.
Kang
Minho
M
Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298, USA.
Akbarali
Hamid I
HI
D000818:Animals; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D051379:Mice; D009130:Muscle, Smooth / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D010766:Phosphorylation; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000096:biosynthesis
C079146:CACNA1C protein, human; D020746:Calcium Channels, L-Type; D009566:Nitrates; C002744:3-nitrotyrosine; D014443:Tyrosine