title: COOH-terminal truncated HBV X protein plays key role in hepatocarcinogenesis.
abstract: OBJECTIVE
  X protein (HBx), a product of hepatitis B virus, has been closely associated with the development of hepatocellular carcinoma (HCC). Based on observations that the COOH-terminal truncated HBx was frequently detected in HCC, the aim of this study is to evaluate the function of COOH-terminal truncated HBx in hepatocarcinogenesis.
  
  
  METHODS
  Expression pattern of HBx was analyzed by immunohistochemistry on tissue microarray containing 194 pairs of HCCs and their matched nontumor liver tissues. MIHA and HepG2 cells transfected with full-length (X2) and COOH-terminal truncated HBx (X1) were tested for their ability to grow in soft agar and form tumors in vivo. Proliferation and apoptosis were assessed using 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide inner salt and terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling assays, respectively. To gain additional insight, the expression profile of HepG2-X2 and HepG2-X1 were compared using cDNA microarray.
  
  
  RESULTS
  COOH-terminal truncated HBx was frequently detected in HCCs (79.3%, n = 111), and our in vitro and in vivo studies showed that the truncated rather than the full-length HBx could effectively transform immortalized liver cell line MIHA. Interestingly, expression profiling revealed differential expression of key genes implicated in the control of cell cycle and apoptosis.
  
  
  CONCLUSIONS
  These findings strongly suggest that the COOH-terminal truncated HBx plays a critical role in the HCC carcinogenesis via the activation of cell proliferation.
authors.affiliation: State Key Laboratory of Oncology in Southern China, Cancer Center, Sun Yat-sen University, 651 East Dongfeng Road, Guangzhou, China.
authors.forename: Ning-Fang
authors.initials: NF
authors.lastname: Ma
authors.forename: Sze Hang
authors.initials: SH
authors.lastname: Lau
authors.forename: Liang
authors.initials: L
authors.lastname: Hu
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Meng-Chao
authors.initials: MC
authors.lastname: Wu
authors.forename: Jackie
authors.initials: J
authors.lastname: Fung
authors.forename: Xueyan
authors.initials: X
authors.lastname: Bai
authors.forename: Chi-Hung
authors.initials: CH
authors.lastname: Tzang
authors.forename: Li
authors.initials: L
authors.lastname: Fu
authors.forename: Mengsu
authors.initials: M
authors.lastname: Yang
authors.forename: Yan An
authors.initials: YA
authors.lastname: Su
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Guan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D005814:Genes, Viral; D006509:Hepatitis B / Q000150:complications; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014412:Tumor Virus Infections / Q000235:genetics / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein