A new lipase isolated from oleaginous seeds from Pachira aquatica (Bombacaceae).
A new lipase from seeds of Pachira aquatica was purified to homogeneity by SDS-PAGE obtaining an enzyme with a molecular weight of approximately 55 kDa. The purified lipase exhibited maximum activity at 40 degrees C and pH 8.0, for an incubation time of 90 min. Concerning temperature stability, at the range from 4 to 50 degrees C, it retained approximately 47% of its original activity for 3 h. The enzyme activity increased in the presence of Ca(++) and Mg(++), but was inhibited by Hg(++), Mn(++), Zn(++), Al(+++) and various oxidizing and reducing agents. The lipase was highly stable in the presence of organic solvents, and its activity was stimulated by methanol. The values of K(m) and V(max) were 1.65 mM and 37.3 micromol mL(-1) min(-1), respectively, using p-nitrophenylacetate as substrate. The enzyme showed preference for esters of long-chain fatty acids, but demonstrated significant activity against a wide range of substrates.
Polizelli
Patricia Peres
PP
Department of Physics, State University of São Paulo, Rua Cristovão Colombo, 2265, São José do Rio Preto, SP CEP 15054-000, Brazil.
Facchini
Fernanda Dell Antonio
FD
Cabral
Hamilton
H
Bonilla-Rodriguez
Gustavo Orlando
GO
D035161:Bombacaceae / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008628:Mercury / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology*; D012997:Solvents / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature; D015032:Zinc / Q000494:pharmacology
D010940:Plant Proteins; D012997:Solvents; D008345:Manganese; D008049:Lipase; D008628:Mercury; D008274:Magnesium; D015032:Zinc; D002118:Calcium; D000432:Methanol