Milk fermented by Lactobacillus gasseri SBT2055 influences adipocyte size via inhibition of dietary fat absorption in Zucker rats.
We have demonstrated previously that a diet containing skimmed milk (SM) fermented by Lactobacillus gasseri SBT2055 (LGSP) reduces adipocyte size in Sprague-Dawley rats. Two experiments were conducted to extend these observations in order to elucidate the mechanism involved. In experiment 1, lean and obese Zucker rats were fed a diet containing SM or LGSP for 4 weeks. The LGSP diet, compared with the SM diet, resulted in lowering of the mesenteric adipose tissue weight (23 %; P < 0.05), adipocyte sizes (28 %; P < 0.001) and serum leptin concentration (36 %; P < 0.05) in lean rats. Obese Zucker rats did not display such dietary effects. Only the number of smaller adipocytes was increased (P < 0.05) by the LGSP diet in the subcutaneous adipose tissue of obese rats. The LGSP diet significantly reduced the serum and hepatic cholesterol in rats. In addition, the LGSP diet led to an increased excretion of faecal fatty acids and total neutral faecal sterols in both rat strains. In experiment 2, Sprague-Dawley rats with permanent cannulation of the thoracic duct were fed either the SM or LGSP diets and their lymph was collected. The LGSP diet lowered the maximum transport rate of TAG and phospholipids. These results indicate that fermented milk regulates adipose tissue growth through inhibition at the stage of dietary fat absorption in lean Zucker rats.
Hamad
Essam M
EM
Laboratory of Nutrition Chemistry, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
Sato
Masao
M
Uzu
Kazunori
K
Yoshida
Takeshi
T
Higashi
Seiichiro
S
Kawakami
Hiroshi
H
Kadooka
Yukio
Y
Matsuyama
Hiroaki
H
Abd El-Gawad
Ibrahim A
IA
Imaizumi
Katsumi
K
D017667:Adipocytes / Q000187:drug effects / Q000473:pathology*; D000273:Adipose Tissue / Q000473:pathology; D050154:Adiposity / Q000187:drug effects; D000824:Animal Nutritional Physiological Phenomena / Q000187:drug effects; D000818:Animals; D048429:Cell Size / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism; D004041:Dietary Fats / Q000493:pharmacokinetics*; D005243:Feces / Q000737:chemistry; D005285:Fermentation; D007408:Intestinal Absorption / Q000187:drug effects; D007778:Lactobacillus; D020738:Leptin / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D008892:Milk / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism / Q000473:pathology*; D009929:Organ Size; D019936:Probiotics / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011924:Rats, Zucker
D004041:Dietary Fats; D020738:Leptin; D002784:Cholesterol