Deficit of Kcnma1 mRNA expression in the dentate gyrus of epileptic rats.
Epileptogenesis in mesial temporal lobe epilepsy is determined by several factors including abnormalities in the expression and function of ion channels. Here, we report a long-lasting deficit in gene expression of Kcnma1 coding for the large-conductance calcium-activated potassium (BK, MaxiK) channel alpha-subunits after pilocarpine-induced status epilepticus. By using comparative real-time PCR, Taqman gene expression assays, and the delta-delta comparative threshold method we detected a significant reduction in Kcnma1 expression in microdissected dentate gyrus at different intervals after status epilepticus (24 h, 10 days, 1 month, and more than 2 months). BK channels are key regulators of neuronal excitability and transmitter release. Hence, defective Kcnma1 expression may play a critical role in the pathogenesis of mesial temporal lobe epilepsy.
Ermolinsky
Boris
B
Department of Biological Sciences, The University of Texas, Brownsville/Texas Southmost College, Brownsville, Texas, USA.
Arshadmansab
Massoud F
MF
Pacheco Otalora
Luis F
LF
Zarei
Masoud M
MM
Garrido-Sanabria
Emilio R
ER
D000704:Analysis of Variance; D000818:Animals; D018891:Dentate Gyrus / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D004833:Epilepsy, Temporal Lobe / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits / Q000235:genetics*; D018721:Muscarinic Agonists / Q000008:administration & dosage / Q000633:toxicity; D010862:Pilocarpine / Q000008:administration & dosage / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012640:Seizures / Q000139:chemically induced / Q000235:genetics; D013226:Status Epilepticus / Q000139:chemically induced / Q000235:genetics; D013997:Time Factors
C494842:Kcnma1 protein, rat; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D018721:Muscarinic Agonists; D012333:RNA, Messenger; D010862:Pilocarpine