title: Production of sophorolipid biosurfactant by Pichia anomala.
abstract: Biosurfactant production by Pichia anomala PY1, a thermotorelant strain isolated from fermented food, was examined as grown in media containing various carbon and nitrogen sources. The optimal conditions for biosurfactant production included 4% soybean oil as carbon source at pH 5.5 at 30 degrees C for 7 d. Under these conditions, the surface tension of the medium decreased to 28 mN/m with oil displacement measured at 69.43 cm(2). Comparative studies of biosurfactant production in media containing glucose or soybean oil were performed. The biosurfactants obtained were isolated and purified by chromatographic methods. The molecular weights of samples were further investigated by mass spectrometry. In medium containing glucose, biosurfactants of molecular weights of 675, 691, and 707 were obtained, while those isolated from medium containing soybean oil were of molecular weights of 658, 675, and 691. These results reveal that sophorolipid compounds containing fatty acids of C20 and C18:1 were produced from both media.
authors.affiliation: Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand. jiraporn.Th@chula.ac.th
authors.forename: Jiraporn
authors.initials: J
authors.lastname: Thaniyavarn
authors.forename: Tanusta
authors.initials: T
authors.lastname: Chianguthai
authors.forename: Polakit
authors.initials: P
authors.lastname: Sangvanich
authors.forename: Niran
authors.initials: N
authors.lastname: Roongsawang
authors.forename: Keji
authors.initials: K
authors.lastname: Washio
authors.forename: Masaaki
authors.initials: M
authors.lastname: Morikawa
authors.forename: Suthep
authors.initials: S
authors.lastname: Thaniyavarn
mesh_terms: D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D013058:Mass Spectrometry; D009584:Nitrogen / Q000378:metabolism; D010843:Pichia / Q000378:metabolism*; D013024:Soybean Oil; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
chemical_list: D008055:Lipids; D013501:Surface-Active Agents; D013024:Soybean Oil; D009584:Nitrogen