title: Conformational analysis of a covalently cross-linked Watson-Crick base pair model.
abstract: Low-temperature NMR experiments and molecular modeling have been used to characterize the conformational behavior of a covalently cross-linked DNA base pair model. The data suggest that Watson-Crick or reverse Watson-Crick hydrogen bonding geometries have similar energies and can interconvert at low temperatures. This low-temperature process involves rotation about the crosslink CH(2)C(5') (psi) carbon-carbon bond, which is energetically preferred over the alternate CH(2)N(3) (phi) carbon-nitrogen bond rotation.
authors.affiliation: Department of Chemistry, Pomona College, 645 North College Avenue, Claremont, CA 91711, USA.
authors.forename: Erik A
authors.initials: EA
authors.lastname: Jensen
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Allen
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Kishi
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: O'Leary
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D013696:Temperature
chemical_list: D004247:DNA