title: In vitro and in vivo behaviour of zinc-doped phosphosilicate glasses.
abstract: The aim of this work was to study the behaviour of zinc-doped phosphosilicate glasses based on Bioglass 45S5. In vitro (in simulated body fluid), the reactivity was analysed by means of inductively coupled plasma spectrometry, environmental scanning electron microscopy-energy-dispersive spectroscopy (ESEM-EDS) and X-ray diffraction. In vivo (a rat implanted with glass), the reactivity and the tissue behaviour were analysed by conventional histology, histochemistry, microradiography and ESEM-EDS. The in vivo behaviour matches that in vitro perfectly; they show comparable glass degradation processes and rates, ruled by the amount of zinc in the glass. The reaction mechanism for the formation of a polymerized silica layer superimposed with a peripheral calcium phosphate layer is clearly substantiated by ESEM-EDS investigations. The crystallization of a biologically active hydroxyapatite (HA) layer is observed in both cases; the in vitro experiment shows the presence of HA after 4 days.
authors.affiliation: Department of Chemistry, University of Modena and Reggio Emilia, Via G.Campi 183, 41100 Modena, Italy.
authors.forename: Gigliola
authors.initials: G
authors.lastname: Lusvardi
authors.forename: Davide
authors.initials: D
authors.lastname: Zaffe
authors.forename: Ledi
authors.initials: L
authors.lastname: Menabue
authors.forename: Carlo
authors.initials: C
authors.lastname: Bertoldi
authors.forename: Gianluca
authors.initials: G
authors.lastname: Malavasi
authors.forename: Ugo
authors.initials: U
authors.lastname: Consolo
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D017553:Phosphorus Compounds / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017640:Silicates / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013997:Time Factors; D015032:Zinc / Q000737:chemistry*
chemical_list: D002130:Calcium Phosphates; D017553:Phosphorus Compounds; D017640:Silicates; D012822:Silicon Dioxide; D017886:Durapatite; C020243:calcium phosphate; D015032:Zinc