Uptake of long chain fatty acids is regulated by dynamic interaction of FAT/CD36 with cholesterol/sphingolipid enriched microdomains (lipid rafts).
BACKGROUND
Mechanisms of long chain fatty acid uptake across the plasma membrane are important targets in treatment of many human diseases like obesity or hepatic steatosis. Long chain fatty acid translocation is achieved by a concert of co-existing mechanisms. These lipids can passively diffuse, but certain membrane proteins can also accelerate the transport. However, we now can provide further evidence that not only proteins but also lipid microdomains play an important part in the regulation of the facilitated uptake process.


METHODS
Dynamic association of FAT/CD36 a candidate fatty acid transporter with lipid rafts was analysed by isolation of detergent resistant membranes (DRMs) and by clustering of lipid rafts with antibodies on living cells. Lipid raft integrity was modulated by cholesterol depletion using methyl-beta-cyclodextrin and sphingolipid depletion using myriocin and sphingomyelinase. Functional analyses were performed using an [3H]-oleate uptake assay.


RESULTS
Overexpression of FAT/CD36 and FATP4 increased long chain fatty acid uptake. The uptake of long chain fatty acids was cholesterol and sphingolipid dependent. Floating experiments showed that there are two pools of FAT/CD36, one found in DRMs and another outside of these domains. FAT/CD36 co-localized with the lipid raft marker PLAP in antibody-clustered domains at the plasma membrane and segregated away from the non-raft marker GFP-TMD. Antibody cross-linking increased DRM association of FAT/CD36 and accelerated the overall fatty acid uptake in a cholesterol dependent manner. Another candidate transporter, FATP4, was neither present in DRMs nor co-localized with FAT/CD36 at the plasma membrane.


CONCLUSIONS
Our observations suggest the existence of two pools of FAT/CD36 within cellular membranes. As increased raft association of FAT/CD36 leads to an increased fatty acid uptake, dynamic association of FAT/CD36 with lipid rafts might regulate the process. There is no direct interaction of FATP4 with lipid rafts or raft associated FAT/CD36. Thus, lipid rafts have to be considered as targets for the treatment of lipid disorders.
Ehehalt
Robert
R
Department of Gastroenterology, University Hospital Heidelberg, INF 410, 69120 Heidelberg, Germany. robert_ehehalt@med.uni-heidelberg.de
Sparla
Richard
R
Kulaksiz
Hasan
H
Herrmann
Thomas
T
Füllekrug
Joachim
J
Stremmel
Wolfgang
W
D000469:Alkaline Phosphatase; D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism*; D019556:COS Cells; D002522:Chlorocebus aethiops; D002784:Cholesterol / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D058851:GPI-Linked Proteins; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D021962:Membrane Microdomains / Q000378:metabolism*; D051379:Mice; D019301:Oleic Acid / Q000378:metabolism; D013107:Sphingolipids / Q000096:biosynthesis; D014162:Transfection; D014709:Vero Cells
D018955:CD36 Antigens; D005227:Fatty Acids; D058851:GPI-Linked Proteins; D007527:Isoenzymes; D013107:Sphingolipids; D049452:Green Fluorescent Proteins; D019301:Oleic Acid; D002784:Cholesterol; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental