Simultaneous determination of p-arsanilic acid and roxarsone in feed by liquid chromatography-hydride generation online coupled with atomic fluorescence spectrometry.
A novel, simple and sensitive liquid chromatography-hydride generation online coupled with atomic fluorescence spectrometry (LC-HG-AFS) method was developed for simultaneous determination of p-arsanilic acid (p-ASA) and roxarsone in feed. 20% Methanol aqueous was used as extraction reagent, after preprocessing samples by ultrasonic oscillation, then injected into the chromatography Waters symmetry shield RP18 analytical column (150mm x 4.6mm, 5 microm), finally detected by an atomic fluorescence spectrometer. The calibration curves of analyses were linear over a range of concentrations (0.2-4mg L-1 and the correlation coefficients were higher than 0.9990. The limits of detection were 0.2 mg L-1. The method has been validated by linearity, precision and recovery. p-ASA and roxarsone in feed can be successfully and simultaneously determined using the developed method without a tedious pretreatment procedure.
Liu
Jianjing
J
Institute of Quality Standards and Testing Technology of Agri-products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Yu
Hongxia
H
Song
Haibin
H
Qiu
Jing
J
Sun
Fengmei
F
Li
Ping
P
Yang
Shuming
S
D000821:Animal Feed / Q000032:analysis*; D001147:Arsanilic Acid / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D012406:Roxarsone / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods*
D012406:Roxarsone; D001147:Arsanilic Acid