Diverse bacteria isolated from root nodules of wild Vicia species grown in temperate region of China.
In the present study, a total of 154 bacterial strains isolated from nodules of eighteen Vicia species mainly grown in the temperate Chinese provinces were characterized by ARDRA, ITS PCR-RFLP, BOX-PCR, sequencing of 16S rDNA, nodC, nifH, atpD and glnII, and nodulation tests. The results demonstrated that most of the R. leguminosarum strains were effective microsymbionts of the wild Vicia species, while genomic species related to Rhizobium gallicum, Mesorhizobium huakuii, Ensifer meliloti and Bradyrhizobium spp. were symbiotic bacteria occasionally nodulating with Vicia species. In addition, fourteen strains related to Agrobacterium, Phyllobacterium, Ensifer, Shinella and R. tropici, as well as 22 strains of R. leguminosarum might be nodule endophytes without symbiotic genes. Diverse symbiotic gene lineages were found among the test strains and a strong association was found among the symbiotic gene types and genomic species, indicating the absence of lateral gene transfer. These results greatly enlarged the rhizobial spectrum of Vicia species.
Lei
Xia
X
Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, 100193 Beijing, China.
Wang
En Tao
ET
Chen
Wen Feng
WF
Sui
Xin Hua
XH
Chen
Wen Xin
WX
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics; D002681:China; D004275:DNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D014644:Genetic Variation; D005843:Geography; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D012231:Rhizobium / Q000145:classification / Q000235:genetics; D013559:Symbiosis; D031306:Vicia / Q000145:classification / Q000382:microbiology*
D001426:Bacterial Proteins; D004275:DNA, Ribosomal; D010088:Oxidoreductases; C029130:nitrogenase reductase