PCR-based detection of microcystin-producing cyanobacterial blooms from Central India.
Microcystin synthetase-gene-specific primers were used to identify hepatotoxic microcystin producing genotypes in six Microcystis spp.-dominant water blooms. Four blooms gave positive PCR reaction. They produced microcystin-RR and -LR amounting to 0.037 to 0.095% of the dry mass.
Ghosh
Shubhro Kamal
SK
Department of Biological Sciences, Rani Durgavati University, Jabalpur 482001, India.
Das
Palash Kumar
PK
Bagchi
Suvendra Nath
SN
D001671:Biochemistry / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000458:Cyanobacteria / Q000378:metabolism*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D004784:Environmental Monitoring / Q000379:methods; D005821:Genetic Techniques; D006801:Humans; D007194:India; D052998:Microcystins / Q000737:chemistry / Q000378:metabolism*; D010839:Phytoplankton / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D017931:DNA Primers; D052998:Microcystins; C078588:microcystin; D004247:DNA