title: Comprehensive resequence analysis of a 136 kb region of human chromosome 8q24 associated with prostate and colon cancers.
abstract: Recently, genome-wide association studies have identified loci across a segment of chromosome 8q24 (128,100,000-128,700,000) associated with the risk of breast, colon and prostate cancers. At least three regions of 8q24 have been independently associated with prostate cancer risk; the most centromeric of which appears to be population specific. Haplotypes in two contiguous but independent loci, marked by rs6983267 and rs1447295, have been identified in the Cancer Genetic Markers of Susceptibility project ( http://cgems.cancer.gov ), which genotyped more than 5,000 prostate cancer cases and 5,000 controls of European origin. The rs6983267 locus is also strongly associated with colorectal cancer. To ascertain a comprehensive catalog of common single-nucleotide polymorphisms (SNPs) across the two regions, we conducted a resequence analysis of 136 kb (chr8: 128,473,000-128,609,802) using the Roche/454 next-generation sequencing technology in 39 prostate cancer cases and 40 controls of European origin. We have characterized a comprehensive catalog of common (MAF > 1%) SNPs within this region, including 442 novel SNPs and have determined the pattern of linkage disequilibrium across the region. Our study has generated a detailed map of genetic variation across the region, which should be useful for choosing SNPs for fine mapping of association signals in 8q24 and investigations of the functional consequences of select common variants.
authors.affiliation: Core Genotyping Facility, Advanced Technology Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD 21702, USA. yeagerm@mail.nih.gov
authors.forename: Meredith
authors.initials: M
authors.lastname: Yeager
authors.forename: Nianqing
authors.initials: N
authors.lastname: Xiao
authors.forename: Richard B
authors.initials: RB
authors.lastname: Hayes
authors.forename: Pascal
authors.initials: P
authors.lastname: Bouffard
authors.forename: Brian
authors.initials: B
authors.lastname: Desany
authors.forename: Laura
authors.initials: L
authors.lastname: Burdett
authors.forename: Nick
authors.initials: N
authors.lastname: Orr
authors.forename: Casey
authors.initials: C
authors.lastname: Matthews
authors.forename: Liqun
authors.initials: L
authors.lastname: Qi
authors.forename: Andrew
authors.initials: A
authors.lastname: Crenshaw
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Markovic
authors.forename: Karin M
authors.initials: KM
authors.lastname: Fredrikson
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Jacobs
authors.forename: Laufey
authors.initials: L
authors.lastname: Amundadottir
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Jarvie
authors.forename: David J
authors.initials: DJ
authors.lastname: Hunter
authors.forename: Robert
authors.initials: R
authors.lastname: Hoover
authors.forename: Gilles
authors.initials: G
authors.lastname: Thomas
authors.forename: Timothy T
authors.initials: TT
authors.lastname: Harkins
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Chanock
mesh_terms: D016022:Case-Control Studies; D002898:Chromosomes, Human, Pair 8; D003110:Colonic Neoplasms / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011471:Prostatic Neoplasms / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods