title: Cell signaling directing the formation and function of hemogenic endothelium during murine embryogenesis.
abstract: During developmental hematopoiesis, multilineage hematopoietic progenitors are thought to derive from a subset of vascular endothelium. Herein, we define the phenotype of such hemogenic endothelial cells and demonstrate, on a clonal level, that they exhibit multilineage hematopoietic potential. Furthermore, we have begun to define the molecular signals that regulate their development. We found that the formation of yolk sac hemogenic endothelium and its hematopoietic potential were significantly impaired in the absence of retinoic acid (RA) signaling, and could be restored in RA-deficient (Raldh2(-/-)) embryos by provision of exogenous RA in utero. Thus, we identify a novel, critical role for RA signaling in the development of hemogenic endothelium that contributes to definitive hematopoiesis.
authors.affiliation: Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Lauren C
authors.initials: LC
authors.lastname: Goldie
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Lucitti
authors.forename: Mary E
authors.initials: ME
authors.lastname: Dickinson
authors.forename: Karen K
authors.initials: KK
authors.lastname: Hirschi
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics; D000818:Animals; D002450:Cell Communication; D019070:Cell Lineage; D004727:Endothelium / Q000166:cytology* / Q000196:embryology*; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D006410:Hematopoiesis; D006579:Heterozygote; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D015398:Signal Transduction; D013997:Time Factors; D014212:Tretinoin / Q000378:metabolism
chemical_list: D014212:Tretinoin; D000445:Aldehyde Oxidoreductases; C470579:RALDH2 protein, mouse