Blood F2-isoprostanes are significantly associated with abnormalities of lipid status in rats with steatosis.
OBJECTIVE
To investigate oxidative stress and lipid peroxidation in hepatic steatosis and the underlying implications in pathological mechanisms of non-alcoholic fatty liver disease (NAFLD).


METHODS
F(2)-isoprostanes (iPF(2alpha)-III) in blood and liver samples from steatotic (n = 9) and control (n = 7) rats were measured as in vivo marker of lipid peroxidation by a mass spectrometric approach. The lipid profile and endogenous antioxidant status (SOD and CAT) in the rats were also analyzed.


RESULTS
Significantly higher levels of iPF(2alpha)-III (mean 3.47 vs 2.40 pmol/mg tissue, P = 0.004) and lower activities of SOD (mean 1.26 U vs 1.40 U, P < 0.001) and CAT (mean 1026.36 U/mg vs 1149.68 U/mg protein, without significance) were observed in the livers of steatotic rats. Plasma total iPF(2alpha)-III was significantly correlated with the abnormalities of blood lipids as well as alanine aminotransferase (ALT) levels in the rats with simple steatosis, whereas no similar tendencies were observed in the control rats.


CONCLUSIONS
Enhancement of hepatic oxidative imbalance occurring at the steatotic stage of NAFLD suggests a possibility that manifestation of the local oxidative damage precedes that of systemic oxidative imbalance. Predominant metabolic features of the increased lipid peroxidation further suggest a close association of the oxidative imbalance and the dyslipidemia with functional deterioration of the steatotic liver. The findings need to be further evaluated, especially in human studies.
Zhu
Man-Jie
MJ
Shanghai Institute of Brain Functional Genomics, East China Normal University, 3663 Zhongshan North Road, Shanghai, China.
Sun
Li-Juan
LJ
Liu
Yue-Qin
YQ
Feng
Yan-Ling
YL
Tong
Hai-Tao
HT
Hu
Ying-He
YH
Zhao
Zheng
Z
D000818:Animals; D015415:Biomarkers / Q000097:blood; D001835:Body Weight / Q000502:physiology; D004195:Disease Models, Animal; D028441:F2-Isoprostanes / Q000097:blood*; D005234:Fatty Liver / Q000097:blood* / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000502:physiology; D008055:Lipids / Q000097:blood*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D028441:F2-Isoprostanes; D008055:Lipids