title: The structure and proinflammatory activity of the lipopolysaccharide from Burkholderia multivorans and the differences between clonal strains colonizing pre and posttransplanted lungs.
abstract: The Burkholderia cepacia complex is a group of Gram-negative bacteria that are opportunistic pathogens for humans especially in cystic fibrosis patients. Lipopolysaccharide (LPS) molecules are potent virulence factors of Gram-negative bacteria organisms essential for bacterial survival. A complete analysis of the bacterial lipopolysaccharide structure to function relationship is required to understand the chemical basis of the inflammatory process. We have therefore investigated the structures of lipopolysaccharides from clonally identical Burkholderia multivorans strains (genomovar II) isolated pre- and post-lung transplantation through compositional analysis, mass spectrometry, and 2D NMR spectroscopy. We tested the LPS proinflammatory activity as a stimulant of human myelomonocytic U937 cell cytokine induction and assessed TLR4/MD2 signaling. Marked changes between the paired strains were found in the lipid A-inner core region. Such structural variations can contribute to the bacterial survival and persistence of infections despite the loss of a CF milieu following lung transplantation.
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Napoli, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ieranò
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.forename: Luisa
authors.initials: L
authors.lastname: Sturiale
authors.forename: Domenico
authors.initials: D
authors.lastname: Garozzo
authors.forename: Heather
authors.initials: H
authors.lastname: Brookes
authors.forename: C M Anjam
authors.initials: CM
authors.lastname: Khan
authors.forename: Clare
authors.initials: C
authors.lastname: Bryant
authors.forename: F Kate
authors.initials: FK
authors.lastname: Gould
authors.forename: Paul A
authors.initials: PA
authors.lastname: Corris
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.forename: Anthony
authors.initials: A
authors.lastname: De Soyza
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
mesh_terms: D042602:Burkholderia cepacia complex / Q000378:metabolism* / Q000472:pathogenicity; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000276:immunology / Q000382:microbiology; D006801:Humans; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology*; D016040:Lung Transplantation / Q000276:immunology*; D051218:Lymphocyte Antigen 96 / Q000378:metabolism; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013329:Structure-Activity Relationship; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014162:Transfection; D020298:U937 Cells
chemical_list: C495377:LY96 protein, human; D008070:Lipopolysaccharides; D051218:Lymphocyte Antigen 96; D051197:Toll-Like Receptor 4