title: Temperature-responsive glass coverslips with an ultrathin poly(N-isopropylacrylamide) layer.
abstract: A temperature-responsive cross-linked polymer gel was covalently grafted onto glass coverslips by electron beam irradiation. The grafted thickness and amount of polymer as well as the surface wettability increased with the initial monomer concentration. When the monomer concentration was 5 wt.%, the grafted polymer density was 0.84microgcm(-2), and cells adhered and spread on the surface at 37 degrees C, but detached at 20 degrees C. In contrast, when the monomer concentration was 35 wt.%, the polymer density was 1.28microgcm(-2), and the surfaces were cell repellent even at 37 degrees C. These results show a remarkable contrast to those obtained from temperature-responsive polymer-grafted tissue culture polystyrene dishes, since various types of cells showed temperature-dependent cell adhesion/detachment when the grafted density was around 2microgcm(-2) on these surfaces. We discuss the possible molecular mechanisms underlying this discrepancy.
authors.affiliation: Department of Applied Chemistry, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo 169-8555, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Fukumori
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Sakai
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004583:Electrons; D005898:Glass; D008422:Materials Testing; D008853:Microscopy / Q000295:instrumentation*; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D001672:Biocompatible Materials; D011108:Polymers; C052970:poly-N-isopropylacrylamide