Asthma induction in mice leads to appearance of alpha2-3- and alpha2-6-linked sialic acid residues in respiratory goblet-like cells.
Allergic asthmatic inflammation in mice was induced by sensitization with ovalbumin and lipopolysaccharide from Escherichia coli and visualized in the airways of asthmatic mice by spatial and temporal changes of carbohydrates containing sialic acid residues. Immunohistochemistry was used to demonstrate binding of lectins and antibodies that detect alpha2-3- and alpha2-6-linked sialic acid residues. After sensitization and challenge, the histology of the lung changed markedly, and goblet-like cells appeared, most likely caused by Clara cell metaplasia. Normal Clara cells showed no reaction after incubation with the sialic acid detecting agents, while the goblet-like cells expressed both alpha2-3- and alpha2-6-linked sialic acid residues in the asthmatic animals. The lectins but not the antibodies reacted with intestinal goblet cells. Instead, an antibody recognizing a disialoganglioside, stained large mononuclear cells in the submucosa, indicating a difference in sialylation between goblet cells in the intestine and goblet-like cells developed from Clara cells.
Kirkeby
Svend
S
Dental School, University of Copenhagen, Copenhagen, Denmark. sk@odont.ku.dk
Jensen
Niels-Erik Viby
NE
Mandel
Ulla
U
Poulsen
Steen Seier
SS
D000818:Animals; D001249:Asthma / Q000276:immunology / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D020397:Goblet Cells / Q000737:chemistry* / Q000276:immunology; D007422:Intestines / Q000166:cytology / Q000276:immunology; D008168:Lung / Q000166:cytology*; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D012794:Sialic Acids / Q000032:analysis* / Q000378:metabolism
D012794:Sialic Acids; D010047:Ovalbumin