SSM-based electrophysiology.
An assay technique for the electrical characterization of electrogenic transport proteins on solid supported membranes is presented. Membrane vesicles, proteoliposomes or membrane fragments containing the transporter are adsorbed to the solid supported membrane and are activated by providing a substrate or a ligand via a rapid solution exchange. This technique opens up new possibilities where conventional electrophysiology fails like transporters or ion channels from bacteria and from intracellular compartments. Its rugged design and potential for automation make it suitable for drug screening.
Schulz
Patrick
P
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max von Laue Str. 3, D-60438 Frankfurt/Main, Germany.
Garcia-Celma
Juan J
JJ
Fendler
Klaus
K
D000818:Animals; D055592:Biophysical Phenomena; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D017136:Ion Transport / Q000502:physiology; D008565:Membrane Proteins / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D027981:Symporters / Q000502:physiology*
D008565:Membrane Proteins; D011994:Recombinant Proteins; D027981:Symporters