title: [Study of the HLA-A, B, DRB1 allele and haplotype frequencies of 4194 cord blood samples in Guangzhou].
abstract: OBJECTIVE
  To analyze the distribution of the human leukocyte antigen (HLA)-A, B and DRB1 allele and haplotype in cord blood samples preserved in Guangzhou Cord Blood Bank collected in the last 10 years.
  
  
  METHODS
  The HLA-A, B and DRB1 genotyping of 4194 cord blood samples were detected by Special Monoclonal Tray, PCR-sequence specific promer (PCR-SSP), PCR-sequence specific oligonucleotide probe (PCR-SSO) and sequence based typing (SBT). Frequencies of HLA-A, B and DRB1 allele and haplotype were calculated by Arlequin software.
  
  
  RESULTS
  The total numbers of HLA-A, B and DRB1 alleles are 18, 43, 13 respectively. The obviously high frequency alleles are A*11, A*02, A*24, A*33, B*40, B*15, B*46, B*13, DRB1*12, DRB1*15, DRB1*09 and DRB1*04, with accumulative frequency of each locus being more than 50%. The most common haplotypes are A2-B46, B46-DR9, A11-DR12 and A2-B46-DR9.
  
  
  CONCLUSIONS
  The distribution of HLA-A, B and DRB1 allele and haplotype of cord blood in Guangzhou Cord Blood Bank has typical characteristics of southern Chinese Han population. Authors' data may help in searching for appropriate donors.
authors.affiliation: Guangzhou Maternal and Fetal Hospital, Guangzhou Medical College, Guangzhou, Guangdong, 510180 People's Republic of China.
authors.forename: Wen-qiong
authors.initials: WQ
authors.lastname: Sha
authors.forename: Can
authors.initials: C
authors.lastname: Liao
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Xue-wei
authors.initials: XW
authors.lastname: Tang
authors.forename: Yi-ning
authors.initials: YN
authors.lastname: Huang
authors.forename: Xing-mei
authors.initials: XM
authors.lastname: Xie
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics; D006239:Haplotypes; D006801:Humans
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens