title: Determination of critical eluent composition for polyethylenglycols using on-line liquid chromatography-Fourier transform infrared spectrometry.
abstract: In this work, it has been extended to methanol:water mobile phases, the use of a background correction method for on-line LC-FTIR measurements named Univariate background correction based on the use of a reference spectra matrix (UBC-RSM) and absorbance ratios. It permits to overcome the problem related to spectral changes occurring during the gradient elution, which in the past limited the on-line coupling of LC and FTIR to isocratic elutions. The combined use of the aforementioned background correction technique in on-line isocratic and gradient LC-FTIR, and partial least squares (PLS) has been applied for the search of the critical conditions for polymers. Polyethylenglycol (PEG) has been used as a model example and results found fitted well with previously published ones.
authors.affiliation: Analytical Chemistry Department, Universitat de València, Edifici Jeroni Muñoz, 50th Dr. Moliner, 46100 Burjassot, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Kuligowski
authors.forename: G
authors.initials: G
authors.lastname: Quintás
authors.forename: S
authors.initials: S
authors.lastname: Garrigues
authors.forename: M
authors.initials: M
authors.lastname: de la Guardia