Effect of different incubation atmospheres on the production of biofilm in methicillin-resistant Staphylococcus aureus (MRSA) grown in nutrient-limited medium.
Today, methicillin-resistant Staphylococcus aureus (MRSA) is a feared cause of nosocomial infections worldwide. These organisms can gain increased resistance to antimicrobial agents through biofilm formation, which appears to be a bacterial survival strategy. MRSA isolates obtained from patients were cultured in nutrient-limited medium supplemented with 0.2% glucose in aerobic, anaerobic, and CO(2) incubation atmospheres. Biofilm formation was quantified by the microtiter plate test. MRSA strains showed significantly lower biofilm production when grown in an aerobic atmosphere compared to that exhibited in CO(2)-rich environments. Gaseous conditions and growth in a nutritionally limited medium can profoundly influence the amount of biofilm formation in MRSA. This should be considered in any in vitro study of in vivo behavior.
Ursic
Viktor
V
Laboratory for Respiratory Microbiology, University Clinic of Respiratory and Allergic Diseases Golnik, Golnik 36, 4204, Golnik, Slovenia. viktor.ursic@klinika-golnik.si
Tomic
Viktorija
V
Kosnik
Mitja
M
D000332:Aerobiosis; D000693:Anaerobiosis; D001431:Bacteriological Techniques; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002245:Carbon Dioxide / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D016106:Methicillin Resistance; D010100:Oxygen / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification
D003470:Culture Media; D002245:Carbon Dioxide; D010100:Oxygen