title: SERRS-based enzymatic probes for the detection of protease activity.
abstract: Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be "switched off" and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml-1.
authors.affiliation: Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, G1 1XL, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Ingram
authors.forename: Louise
authors.initials: L
authors.lastname: Byers
authors.forename: Karen
authors.initials: K
authors.lastname: Faulds
authors.forename: Barry D
authors.initials: BD
authors.lastname: Moore
authors.forename: Duncan
authors.initials: D
authors.lastname: Graham
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D008049:Lipase / Q000032:analysis / Q000378:metabolism; D010447:Peptide Hydrolases / Q000032:analysis* / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000596:Amino Acids; D008049:Lipase; D010447:Peptide Hydrolases