title: DNA sequence analysis of varicella-zoster virus gene 62 from subclinical infections in healthy children immunized with the Oka varicella vaccine.
abstract: A live attenuated varicella vaccine, the Oka vaccine strain (vOka), is routinely administered to children in Japan and other countries, including the United States. vOka consists of a mixture of genotypically distinct variants, but little is known about the growth potential of each variants in vivo. We isolated varicella-zoster virus (VZV) DNA sequences from the peripheral blood mononuclear cells (PBMCs) of asymptomatic healthy children immunized with the Oka varicella vaccine. VZV gene 62 DNA fragments were detected in 5 of 166 (3.0%) PBMC samples by nested PCR within 5 weeks of the vaccination. Sequence analysis of VZV DNA from these five PBMC samples indicated that multiple viral clones in the vaccine could infect vaccinees and replicate in vivo. We also provide evidence that a nonsynonymous substitution at position 105356 may affect viral replication in vivo.
authors.affiliation: Kanonji Institute, Research Foundation for Microbial Diseases of Osaka University, 2-9-41, Yahata-cho, Kanonji, Kagawa, Japan. ygomi@mail.biken.or.jp
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Gomi
authors.forename: Takao
authors.initials: T
authors.lastname: Ozaki
authors.forename: Naoko
authors.initials: N
authors.lastname: Nishimura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Narita
authors.forename: Michio
authors.initials: M
authors.lastname: Suzuki
authors.forename: Jaekun
authors.initials: J
authors.lastname: Ahn
authors.forename: Naoko
authors.initials: N
authors.lastname: Watanabe
authors.forename: Norio
authors.initials: N
authors.lastname: Koyama
authors.forename: Hajime
authors.initials: H
authors.lastname: Ushida
authors.forename: Naoko
authors.initials: N
authors.lastname: Yasuda
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Nakane
authors.forename: Keiji
authors.initials: K
authors.lastname: Funahashi
authors.forename: Isao
authors.initials: I
authors.lastname: Fuke
authors.forename: Akihisa
authors.initials: A
authors.lastname: Takamizawa
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamanishi
authors.forename: Michiaki
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002644:Chickenpox / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control / Q000821:virology*; D019433:Chickenpox Vaccine / Q000008:administration & dosage*; D002648:Child; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics*; D007114:Immunization; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D019433:Chickenpox Vaccine; C074221:IE62 protein, Human herpesvirus 3; D017874:Immediate-Early Proteins; D015534:Trans-Activators; D014759:Viral Envelope Proteins