[Effect of salidroside on salivary adenoid cystic carcinoma cells in vitro].
OBJECTIVE
To study the effect and mechanism of salidroside on salivary adenoid cystic carcinoma (SACC-2) cells in vitro.


METHODS
To detect the effect of salidroside on SACC-2 cells growth with CCK-8 kit, the growth curve of cells were drawn. To detect the expression of cysteine proteinase (Caspase 3 and Caspase 8) and proliferating cell nuclear antigen (PCNA) in SACC-2 cells, immunohistochemistry staining was used.


RESULTS
According the results of CCK-8 kit detected, salidroside could inhibit the proliferation of SACC-2 cells, IC50 value was (4.99+/-0.23) microg/mL. Growing curve showed that SACC-2 cells of salidroside groups decreased with extending cell culture time. Immunohistochemistry staining showed that Caspase 3 and Caspase 8 were both strong positive expression in SACC-2 cells of salidroside groups, and poor positive expression in SACC-2 cells of control group, the difference was significant (P<0.01). The expression of PCNA was reverse (P<0.01).


CONCLUSIONS
Salidroside could inhibit the proliferation of SACC-2 cells and induce SACC-2 cell apoptosis in vitro, which could be a kind of antitumor medicine in the future.
Li
Mei-Hua
MH
Department of Stomatology, The Third Hospital of Jilin University, Changchun 130031, China.
Zhang
Gui-Zhen
GZ
Wang
Yi-Shu
YS
D017209:Apoptosis; D003528:Carcinoma, Adenoid Cystic; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005960:Glucosides; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D010636:Phenols; D012468:Salivary Gland Neoplasms
D005960:Glucosides; D010636:Phenols; C009172:rhodioloside