Expression of canine Kdap in normal, hyperplastic and neoplastic epidermis.
Keratinocyte differentiation-associated protein, Kdap, is a recently identified small secretory protein that may act as a soluble regulator for the cornification and/or desquamation of keratinocytes. To clarify the role of Kdap in the terminal differentiation of keratinocytes, detailed in situ localisation of Kdap was studied using canine skin with normal, hyperplastic and neoplastic epidermis. In normal canine trunk skin, Kdap was expressed by granular keratinocytes, with polarity to the apical side of the cells, suggesting that canine Kdap is present in lamellar granules, as in humans. Expression of Kdap was widespread in the spinous layers in hyperplastic epidermis, but was undetectable in squamous cell carcinomas. These findings suggest that Kdap is closely related to the delay of terminal differentiation and/or release of cells in hyperplastic epidermis.
Yagihara
Hiroko
H
Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
Kinjo
Emiko
E
Kobayashi
Yohei
Y
Tsuji
Ayaka
A
Nishimura
Yudai
Y
Shinozaki
Nao
N
Tamura
Kyoichi
K
Isotani
Mayu
M
Nakagaki
Kazuhide
K
Takahashi
Kimimasa
K
Ono
Kenichiro
K
Washizu
Tsukimi
T
Bonkobara
Makoto
M
D000818:Animals; D002460:Cell Line; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006965:Hyperplasia / Q000201:enzymology / Q000235:genetics / Q000662:veterinary*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012878:Skin Neoplasms / Q000201:enzymology* / Q000235:genetics
D010750:Phosphoproteins; D012333:RNA, Messenger