Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0.
Amycolatopsis sp. AB0, a copper resistant actinobacterium isolated from polluted sediments, has shown high copper specific biopsortion ability (25 mg g(-1)). Two approaches were used to confirm metal accumulation in growing cells of Amycolatopsis sp. AB0; we performed subcellular fractioning assays which showed that the retained copper was associated with the extra-cellular fraction (exopolymer, 40%), but mainly within the cells. Intracellular distribution of copper was: 86% in the cytosolic fraction, 11% at the cell wall and 3% associated with the ribosome/membrane fraction. Its copper bioaccumulation ability was corroborated by using silver enhanced staining of copper with the Timm's reagent technique, which has not been used to detect metal deposits in bacteria before. In addition, we constructed specific oligonucleotides for targeting genes coding for copper P-Type ATPases that could be involved in the copper uptake ability of this strain. A 607 bp DNA fragment was amplified and sequenced from Amycolatopsis sp AB0. BLAST search analysis showed 71% protein homology of the deduced sequence with a putative cation-transporting ATPase of Nocardia farcinica and 65% with a copper translocating ATPase of Mycobacterium flavescens. To our knowledge this is the first report of the presence of copper P-type ATPase genes in the Amycolotopsis genus.
Albarracín
Virginia Helena
VH
Pilot Plant of Industrial and Microbiological Processes (PROIMI), CONICET, Av. Belgrano y Pasaje Caseros, Tucumán, Argentina.
Winik
Beatriz
B
Kothe
Erika
E
Amoroso
María Julia
MJ
Abate
Carlos Mauricio
CM
D000192:Actinomycetales / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D000073840:Copper-Transporting ATPases; D004269:DNA, Bacterial / Q000235:genetics; D018390:Gene Targeting; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D004269:DNA, Bacterial; D003300:Copper; D000251:Adenosine Triphosphatases; D000073840:Copper-Transporting ATPases