title: Immunohistochemical characterization of nasal-type extranodal NK/T-cell lymphoma using a tissue microarray: an analysis of 84 cases.
abstract: Nasal-type extranodal natural killer (NK)/T-cell lymphoma is an uncommon malignancy. By using a tissue microarray, we characterized 84 cases of extranodal NK/T-cell lymphoma with regard to expression of 18 immunohistochemical markers and the presence of Epstein-Barr virus (EBV) RNA. In our series, CD2 was positive in 69 (93%) of 74 cases, CD3 in 68 (84%) of 81, CD5 in 22 (27%) of 81, CD20 in 0 (0%) of 82, CD29 in 75 (91%) of 82, CD30 in 29 (35%) of 84, CD43 in 81 (96%) of 84, CD54 in 58 (72%) of 81, CD56 in 46 (58%) of 79, CD62L in 23 (28%) of 83, CD183 in 66 (80%) of 83, BCL2 in 33 (39%) of 84, cutaneous lymphocyte antigen in 21 (25%) of 84, granzyme B in 70 (83%) of 84, Ki-67 in 59 (71%) of 83, linker for activation of T cells in 60 (71%) of 84, perforin in 66 (86%) of 77, TIA1 in 76 (90%) of 84, and EBV in 73 (87%) of 84. Hierarchical cluster analysis separated primary cutaneous cases from cases manifesting in other sites based on lower expression of the cell adhesion molecule CD54.
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305-5324, USA.
authors.forename: Erich J
authors.initials: EJ
authors.lastname: Schwartz
authors.forename: Hernan
authors.initials: H
authors.lastname: Molina-Kirsch
authors.forename: Shuchun
authors.initials: S
authors.lastname: Zhao
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Marinelli
authors.forename: Roger A
authors.initials: RA
authors.lastname: Warnke
authors.forename: Yasodha
authors.initials: Y
authors.lastname: Natkunam
mesh_terms: D020031:Epstein-Barr Virus Infections / Q000473:pathology*; D004854:Herpesvirus 4, Human; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D017403:In Situ Hybridization; D007694:Killer Cells, Natural / Q000473:pathology; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D016411:Lymphoma, T-Cell, Peripheral / Q000473:pathology* / Q000821:virology; D046228:Microarray Analysis; D009669:Nose Neoplasms / Q000473:pathology* / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D013602:T-Lymphocytes, Cytotoxic / Q000473:pathology
chemical_list: D012367:RNA, Viral; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human