Label-free, all-electrical, in situ human epidermal growth receptor 2 detection.
Using 3-mercaptopropyltrimethoxysilane (MPS)-coated (PbMg 1/3 Nb 2/3 O3)0.63-(PbTiO3)0.37 (PMN-PT)/tin and lead zirconate titanate/glass piezoelectric microcantilever sensors (PEMSs) with single-chain variable fragment (scFv) immobilized on the MPS surface, we have demonstrated real-time, label-free detection of human epidermal growth factor receptor 2 (Her2) in a background of 1 mg/ml bovine serum albumin. Coupled with a scFv with a KD of 3.4 x 10(-8)M, the MPS-insulated PMN-PT/tin PEMS 560 microm long and 720 microm wide exhibited a Her2 concentration sensitivity of 5 ng/ml in a background of 1 mg/ml BSA.
Capobianco
Joseph A
JA
Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USA.
Shih
Wan Y
WY
Yuan
Qing-An
QA
Adams
Gregory P
GP
Shih
Wei-Heng
WH
D000818:Animals; D002417:Cattle; D002460:Cell Line; D004867:Equipment Design; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005898:Glass; D006801:Humans; D007700:Kinetics; D007854:Lead / Q000737:chemistry*; D017646:Organosilicon Compounds; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis* / Q000096:biosynthesis; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
D007854:Lead; D017646:Organosilicon Compounds; D018719:Erb-b2 Receptor Tyrosine Kinases; D012710:Serum Albumin, Bovine; D012821:Silanes; D014025:Titanium; C481719:lead titanate; C102833:(3-mercaptopropyl)trimethoxysilane; C508053:ERBB2 protein, human