title: Molecular cloning and characterization of proliferating cell nuclear antigen (PCNA) from Chinese shrimp Fenneropenaeus chinensis.
abstract: The proliferating cell nuclear antigen gene was cloned from Fenneropenaeus chinensis (FcPCNA). The full-length cDNA sequence of FcPCNA encodes 260 amino acids showing high identity with PCNAs reported in other species. FcPCNA expressed especially high in proliferating tissues of shrimp such as haematopoietic tissue (HPT) and ovary. In order to understand the response of HPT to bacteria and virus challenge, mRNA level of FcPCNA in HPT was analyzed after shrimp were challenged by Vibrio anguillarum and white spot syndrome virus (WSSV). FcPCNA expression in HPT of shrimp was responsive to WSSV and Vibrio challenge, but different expression profiles were obtained after challenge by these two pathogens. The data provide additional information to understand the defense mechanisms of shrimp against virus and bacteria.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, PR China.
authors.forename: Yusu
authors.initials: Y
authors.lastname: Xie
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Fuhua
authors.initials: F
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Jianhai
authors.initials: J
authors.lastname: Xiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014733:Vibrio / Q000276:immunology / Q000472:pathogenicity; D046848:White spot syndrome virus 1 / Q000276:immunology / Q000472:pathogenicity
chemical_list: D018076:DNA, Complementary; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger