title: Three-dimensional power Doppler sonography of the (sub)endometrium and angiogenic cytokine concentrations.
abstract: The aim of this study was to determine the correlation between three-dimensional power Doppler sonography (3D-PDS) of the (sub)endometrium and concentrations of angiogenic cytokines in patients attending an IVF programme. A total of 42 patients was included in a prospective, non-randomized clinical study. 3D-PDS of the (sub)endometrium was performed on the day of oocyte aspiration, with and without contrast agent. Quantitative assessment included the following 3D Doppler parameters: vascularization index, flow intensity, and vascularization flow index. On the same day, concentrations of oestradiol (serum only), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF) 1, IGF-binding protein 3 (IGFBP-3) and leptin were determined in the serum and in the follicular fluid. All 3D-PDS indices were significantly higher with contrast enhancement (P < 0.05). Follicular fluid concentrations of VEGF and IGFBP-3, as well as serum concentrations of leptin, showed significant P-values when correlated with (sub)endometrial Doppler indices. A weak linear dependency appeared between flow intensity and VEGF and leptin. Furthermore, weak dependencies were apparent between 3D Doppler parameters and high follicular fluid concentrations of VEGF and IGFBP-3. It is concluded that there is only little evidence for an association between (sub)endometrial Doppler indices as assessed by 3D-PDS and concentrations of angiogenic cytokines.
authors.affiliation: Department of Obstetrics and Gynaecology, Mannheim University Hospital, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany. kaiserschnitt@gmx.de
authors.forename: J
authors.initials: J
authors.lastname: Siemer
authors.forename: H
authors.initials: H
authors.lastname: Binder
authors.forename: C
authors.initials: C
authors.lastname: Willeke
authors.forename: C
authors.initials: C
authors.lastname: Dorn
authors.forename: J
authors.initials: J
authors.lastname: Reinsberg
authors.forename: R
authors.initials: R
authors.lastname: Fimmers
authors.forename: H
authors.initials: H
authors.lastname: van der Ven
authors.forename: R L
authors.initials: RL
authors.lastname: Schild
mesh_terms: D000293:Adolescent; D000328:Adult; D042501:Angiogenic Proteins / Q000032:analysis*; D016207:Cytokines / Q000032:analysis* / Q000097:blood; D004717:Endometrium / Q000098:blood supply / Q000000981:diagnostic imaging*; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D015571:Follicular Fluid / Q000737:chemistry; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D018972:Insulin-Like Growth Factor Binding Protein 3; D018969:Insulin-Like Growth Factor Binding Proteins / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D020738:Leptin / Q000097:blood; D010865:Pilot Projects; D011247:Pregnancy; D018608:Ultrasonography, Doppler / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: D042501:Angiogenic Proteins; D016207:Cytokines; C515497:IGFBP3 protein, human; D018972:Insulin-Like Growth Factor Binding Protein 3; D018969:Insulin-Like Growth Factor Binding Proteins; D020738:Leptin; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D004958:Estradiol; D007334:Insulin-Like Growth Factor I