title: Expression of CD40 in ovarian cancer and adenovirus-mediated CD40 ligand therapy on ovarian cancer in vitro.
abstract: OBJECTIVE
  To test the expression level of CD40 on ovarian cancer tissues and its correlation to clinicopathological features of patients and to evaluate the therapeutic effectiveness of adenovirus-mediated CD40 ligand on ovarian cancer in vitro.
  
  
  METHODS
  The expression of CD40 on paraffin-embedded ovarian cancer tissues (n=58) and normal ovarian tissues (n=15) was tested by immunohistochemistry, and CD40 expression on ovarian cancer cells derived from fresh surgical specimens was tested by flow cytometry analysis. The apoptosis-inducing effects of adenovirus-mediated CD40 ligand therapy on ovarian cancer cells derived from fresh surgical specimens were analyzed by flow cytometry analysis and TUNEL assay.
  
  
  RESULTS
  CD40 expression was detected in 60.3% (35/58) of paraffin-embedded ovarian cancer tissues and 73.3% (11/15) of fresh ovarian cancer tissues, but not in normal ovarian tissues (n=15). CD40 expression was significantly correlated with FIGO stage of ovarian cancer. Adenovirus-mediated CD40 ligand therapy induced significant apoptosis effects on ovarian cancer cells derived from fresh surgical specimens in vitro compared to null adenovirus vector and phosphate-buffered saline.
  
  
  CONCLUSIONS
  Our results suggested the therapeutic potential of adenovirus-mediated CD40 ligand on ovarian cancer, especially on the late stage of ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Guizhou Provincial People's Hospital, Guiyang, Guizhou, 550002, People's Republic of China.
authors.forename: Enli
authors.initials: E
authors.lastname: Jiang
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.forename: Xiancheng
authors.initials: X
authors.lastname: Chen
authors.forename: Guojuan
authors.initials: G
authors.lastname: Sun
authors.forename: Hongbing
authors.initials: H
authors.lastname: Wu
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000256:Adenoviridae; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D019013:CD40 Antigens / Q000032:analysis*; D023201:CD40 Ligand / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery
chemical_list: D000970:Antineoplastic Agents; D019013:CD40 Antigens; D023201:CD40 Ligand