title: The cell biology of DNA methylation in mammals.
abstract: In this review, we will provide a brief reminder of epigenetic phenomena in general, and DNA methylation in particular. We will then underline the characteristics of the in vivo organization of the genome that limit the applicability of in vitro results. We will use several examples to point out the connections between DNA methylation and nuclear architecture. Finally, we will outline some of the hopes and challenges for future research in the field. The study of DNA methylation, its effectors, and its roles, illustrates the complementarity of in vitro approaches and cell biology.
authors.affiliation: Center BioEngineering, Russian Academy of Sciences, Prospekt 60-let Oktyabrya 7-1, 117312 Moscow, Russia.
authors.forename: Egor
authors.initials: E
authors.lastname: Prokhortchouk
authors.forename: Pierre-Antoine
authors.initials: PA
authors.lastname: Defossez
mesh_terms: D000818:Animals; D003596:Cytosine / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D004261:DNA Replication; D044127:Epigenesis, Genetic; D005786:Gene Expression Regulation; D008322:Mammals / Q000235:genetics*; D051783:Methyl-CpG-Binding Protein 2 / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000378:metabolism; D009690:Nucleic Acid Conformation; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D051783:Methyl-CpG-Binding Protein 2; D009687:Nuclear Proteins; D014157:Transcription Factors; C118822:Zbtb33 protein, mouse; D003596:Cytosine; D004247:DNA; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases