title: Immunolocalization of androgen receptor in the boar epididymis: the effect of GnRH agonist deslorelin.
abstract: Epididymides from nine crossbred male pigs [Polish Landrace x (Duroc x Pietrain)] (n = 3 per each group) were used in this study to show whether there are any differences between androgen receptor (AR) distribution along epididymal duct of a GnRH agonist deslorelin-treated boars when compared to the control tissues. The active agent was administered by way of a subcutaneous controlled-release implant containing 4.7 mg deslorelin at 91 or 147 days of age respectively. Boars from two experimental groups and the control group were slaughtered at 175 day of age. Immunolocalization was performed using a polyclonal rabbit antiserum against the AR. In control boars, strong staining for AR was detected in nuclei of the epithelial (principal and basal) and stromal cells, whereas in boars treated with deslorelin the staining was confined to the principal cell nuclei. In those treated for 84 days, AR-immunostaining was weak or the principal cells were negative for the AR. Irrespective of the time from deslorelin insertion all stromal cells were immunonegative. The results demonstrate for the first time the effect of deslorelin on the distribution of the AR in the three regions of the boar epididymis. It is likely that stromal cells are more sensitive than epithelial cells to the regulation of AR expression by androgen. The morphological and functional alterations along the epididymal duct and lack of spermatozoa within the lumen after deslorelin treatment indicate that a potent GnRH agonist is likely responsible for an impairment of the microenvironment created by epididymal cells for sperm maturation and their storage.
authors.affiliation: Department of Endocrinology, Institute of Zoology, Jagiellonian University, Krakow, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Kopera
authors.forename: R
authors.initials: R
authors.lastname: Tuz
authors.forename: A
authors.initials: A
authors.lastname: Hejmej
authors.forename: T
authors.initials: T
authors.lastname: Schwarz
authors.forename: J
authors.initials: J
authors.lastname: Koczanowski
authors.forename: B
authors.initials: B
authors.lastname: Bilińska
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D004822:Epididymis / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D004847:Epithelial Cells / Q000648:ultrastructure; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists*; D007150:Immunohistochemistry; D008297:Male; D011944:Receptors, Androgen / Q000032:analysis*; D017154:Stromal Cells / Q000648:ultrastructure; D013552:Swine; D017329:Triptorelin Pamoate / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D011944:Receptors, Androgen; D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone; C066438:deslorelin