Design and synthesis of a novel DNA-encoded chemical library using Diels-Alder cycloadditions.
DNA-encoded chemical libraries are increasingly being employed for the identification of binding molecules to protein targets of pharmaceutical relevance. Here, we describe the synthesis and characterization of a DNA-encoded chemical library, consisting of 4000 compounds generated by Diels-Alder cycloaddition reactions. The compounds were encoded with unique DNA fragments which were generated through a stepwise assembly process and serve as amplifiable bar codes for the identification and relative quantification of library members.
Buller
Fabian
F
Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.
Mannocci
Luca
L
Zhang
Yixin
Y
Dumelin
Christoph E
CE
Scheuermann
Jörg
J
Neri
Dario
D
D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D015723:Gene Library; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis*; D019809:Streptavidin
D009841:Oligonucleotides; D004247:DNA; D019809:Streptavidin