title: Transcriptional profiles in CD8+ T cells from HIV+ progressors on HAART are characterized by coordinated up-regulation of oxidative phosphorylation enzymes and interferon responses.
abstract: The functional impairment and numerical decline of CD8+ T cells during HIV infection has a profound effect on disease progression, but only limited microarray studies have used CD8+ T cells. To understand the interactions of HIV and host CD8+ T cells at different disease status, we used the Illumina Human-6 BeadChips to evaluate the transcriptional profile (>48,000 transcripts) in primary CD8+ T cells from HIV+ therapy-naive non-progressors and therapy-experienced progressors. 68 differentially expressed genes were identified, of which 6 have been reported in HIV context, while others are associated with biological functions relevant to HIV pathogenesis. By GSEA, the coordinated up-regulation of oxidative phosphorylation enzymes and interferon responses were detected as fingerprints in HIV progressors on HAART, whereas LTNP displayed a transcriptional signature of coordinated up-regulation of components of MAPK and cytotoxicty pathways. These results will provide biological insights into natural control of HIV versus HIV control under HAART.
authors.affiliation: Retroviral Genetics Division, Center for Virus Research, Westmead Millennium Institute, University of Sydney, Westmead, NSW, Australia. jingqin_wu@wmi.usyd.edu.au
authors.forename: Jing Qin
authors.initials: JQ
authors.lastname: Wu
authors.forename: Dominic E
authors.initials: DE
authors.lastname: Dwyer
authors.forename: Wayne B
authors.initials: WB
authors.lastname: Dyer
authors.forename: Yee Hwa
authors.initials: YH
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Nitin K
authors.initials: NK
authors.lastname: Saksena
mesh_terms: D023241:Antiretroviral Therapy, Highly Active; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D020869:Gene Expression Profiling; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007372:Interferons / Q000494:pharmacology / Q000627:therapeutic use*; D010085:Oxidative Phosphorylation; D014158:Transcription, Genetic / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D007372:Interferons