title: Saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane.
abstract: A bacterial strain designated GR21T was isolated from apoplastic fluid of Saccharum officinarum (sugar cane). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family 'Paenibacillaceae', with Paenibacillus as the closest related genus. Within this genus, the closest related species is Paenibacillus xylanilyticus, with 93.4 % similarity to the sequence of the type strain. The isolate has Gram-variable, facultatively anaerobic, rod-shaped cells, motile by polar and subpolar flagella. Round, non-ornamented, central or subterminal spores are formed in unswollen sporangia. The strain is catalase-positive and oxidase-negative on nutrient agar medium. Cellulose and aesculin were hydrolysed, whereas xylan, starch and gelatin were not. Growth was supported by many carbohydrates as carbon sources. Strain GR21T displayed a lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, an unknown aminophospholipid, two unknown glycolipids and an unknown phosphoglycolipid. MK-7 was the predominant menaquinone and anteiso-C15: 0 was the major fatty acid. The DNA G+C content was 57.8 mol%. Phylogenetic and phenotypic analyses, including assimilation of carbon sources and exoenzyme production commonly used for classification within the family 'Paenibacillaceae', showed that strain GR21T belongs to a new genus within this family, for which the name Saccharibacillus sacchari gen. nov., sp. nov. is proposed. The type strain of Saccharibacillus sacchari is GR21T (=LMG 24085T =DSM 19268T).
authors.affiliation: Departamento de Microbiología y Genética, Lab 209, Edificio Departamental de Biología Campus Miguel de Unamuno, Universidad de Salamanca, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Rivas
authors.forename: Paula
authors.initials: P
authors.lastname: García-Fraile
authors.forename: José Luis
authors.initials: JL
authors.lastname: Zurdo-Piñeiro
authors.forename: Pedro F
authors.initials: PF
authors.lastname: Mateos
authors.forename: Eustoquio
authors.initials: E
authors.lastname: Martínez-Molina
authors.forename: Eulogio J
authors.initials: EJ
authors.lastname: Bedmar
authors.forename: Juan
authors.initials: J
authors.lastname: Sánchez-Raya
authors.forename: Encarna
authors.initials: E
authors.lastname: Velázquez
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D016987:Gram-Positive Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D031786:Saccharum / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S