title: Fhit proteins can also recognize substrates other than dinucleoside polyphosphates.
abstract: We show here that Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them. With respect to the former, Fhits behave like a phosphodiesterase I concomitant with cleavage of the P-F bond. Some kinetic parameters and implications of the novel reactions catalyzed by the human and plant (Arabidopsis thaliana) Fhit proteins are presented.
authors.affiliation: Department of Biochemistry and Biotechnology, The University of Life Sciences, 60-637 Poznań, Poland. guranowski@au.poznan.pl
authors.forename: Andrzej
authors.initials: A
authors.lastname: Guranowski
authors.forename: Anna M
authors.initials: AM
authors.lastname: Wojdyła
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pietrowska-Borek
authors.forename: Paweł
authors.initials: P
authors.lastname: Bieganowski
authors.forename: Elena N
authors.initials: EN
authors.lastname: Khurs
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Cliff
authors.forename: G Michael
authors.initials: GM
authors.lastname: Blackburn
authors.forename: Damian
authors.initials: D
authors.lastname: Błaziak
authors.forename: Wojciech J
authors.initials: WJ
authors.lastname: Stec
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D029681:Arabidopsis Proteins; D015226:Dinucleoside Phosphates; D009363:Neoplasm Proteins; D010727:Phosphoric Diester Hydrolases; D000099170:Bis(5'-adenosyl)triphosphatase