Peptide mass fingerprinting using isotopically encoded photo-crosslinking amino acids.
When isotopically labelled photo-crosslinking amino acids are site-specifically incorporated into proteins, in combination with the corresponding non-labeled analogue, cross-linked tryptic peptides are easily identified in mass spectra via characteristic "doublet" patterns.
Wilkins
Bryan J
BJ
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Daggett
Kelly A
KA
Cropp
T Ashton
TA
D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry; D007553:Isotope Labeling; D013058:Mass Spectrometry; D008958:Models, Molecular; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods*
D000596:Amino Acids; D003432:Cross-Linking Reagents; D010446:Peptide Fragments