title: Reproducible comet assay of amorphous silica nanoparticles detects no genotoxicity.
abstract: Genotoxicity of commercial colloidal and laboratory-synthesized silica nanoparticles was tested using the single cell gel electrophoresis or Comet assay. By using a carefully developed protocol and careful characterization of the nanoparticle dispersions, Comet assays were performed on 3T3-L1 fibroblasts with 3, 6, and 24 h incubations and 4 or 40 microg/ml of silica nanoparticles. No significant genotoxicity was observed for the nanoparticles tested under the conditions described, and results were independently validated in two separate laboratories, showing that in vitro toxicity testing can be quantitatively reproducible.
authors.affiliation: University of Ulster, Coleraine, United Kingdom.
authors.forename: Clifford A
authors.initials: CA
authors.lastname: Barnes
authors.forename: Andreas
authors.initials: A
authors.lastname: Elsaesser
authors.forename: Joanna
authors.initials: J
authors.lastname: Arkusz
authors.forename: Anna
authors.initials: A
authors.lastname: Smok
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Palus
authors.forename: Anna
authors.initials: A
authors.lastname: Leśniak
authors.forename: Anna
authors.initials: A
authors.lastname: Salvati
authors.forename: John P
authors.initials: JP
authors.lastname: Hanrahan
authors.forename: Wim H de
authors.initials: WH
authors.lastname: Jong
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Dziubałtowska
authors.forename: Maciej
authors.initials: M
authors.lastname: Stepnik
authors.forename: Konrad
authors.initials: K
authors.lastname: Rydzyński
authors.forename: George
authors.initials: G
authors.lastname: McKerr
authors.forename: Iseult
authors.initials: I
authors.lastname: Lynch
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Dawson
authors.forename: C Vyvyan
authors.initials: CV
authors.lastname: Howard
mesh_terms: D020552:Comet Assay; D046529:Microscopy, Electron, Transmission; D009152:Mutagenicity Tests; D053758:Nanoparticles; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide