Short-term overcrowding of Atlantic cod, Gadus morhua: effects on serum-mediated antibacterial activity and transcription of glucose transport and antioxidant defense related genes.
Serum-mediated control of Listonella anguillarum and transcriptional profiles of selected glucose transport and antioxidant defense genes, following short-term overcrowding in Atlantic cod, Gadus morhua were determined. Fish were subjected to overcrowding by reducing the water level in the tank for 1 h and this was repeated thrice over a 12 h period. Blood samples were collected before overcrowding (initial group) and at 2, 24 and 72 h post-crowding. The sera from fish obtained at 2 h post-crowding caused a significant reduction in L. anguillarum counts compared to the initial samples. There was a transcriptional upregulation of the glucose transport-4 and glyceraldehyde-3-phosphate dehydrogenase genes at 2 h after crowding. Gene transcripts of the antioxidant enzymes, Cu/Zn superoxide dismutase (Cu/Zn SOD), catalase and phospholipid hydroperoxide glutathione peroxidase also significantly increased at 2 h post-crowding, but thereafter they returned to their pre-crowding levels with the exception of Cu/Zn SOD that remained significantly higher than the initial group until 72 h. Thus, short-term overcrowding of Atlantic cod leads to a transient enhancement of in vitro serum antibacterial activity and enhanced transcriptional activity of glucose transport and antioxidant defense genes.
Caipang
Christopher Marlowe A
CM
Faculty of Biosciences and Aquaculture, Bodø University College, Bodø 8049, Norway.
Brinchmann
Monica F
MF
Kiron
Viswanath
V
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood; D000975:Antioxidants / Q000378:metabolism; D001483:Base Sequence; D002374:Catalase / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D048389:Gadus morhua / Q000097:blood / Q000235:genetics* / Q000502:physiology*; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000235:genetics; D005979:Glutathione Peroxidase / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D018384:Oxidative Stress; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000235:genetics
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D017931:DNA Primers; D051275:Glucose Transporter Type 4; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005947:Glucose