[Effect of Cryptoporus polysaccharide on lipopolysaccharide-induced production of monocyte chemoattractant protein-1 in human alveolar epithelial cells].
To investigate the effect of cryptoporus polysaccharide(CP)on lipopolysaccharide(LPS)-induced production of monocyte chemoattractant protein-1(MCP-1)in human lung epithelial A549 cells.



A549 cells were stimulated with LPS in the presence or absence of CP. The protein concentration and mRNA expression of MCP-1 were determined by enzyme-linked-immunosobent assay(ELISA)and semi-quantitative RT-PCR, respectively.



The protein concentration of MCP-1 was significantly increased by LPS 1000 microg/L at 24 h. There were no effects on the growth and viability of A549 cells in the presence of CP 100 microg/L or dexamethasone 1 mumol/L. However, CP 100 microg/L or dexamethasone 1 micromol/L significantly inhibited the protein concentration and mRNA expression of MCP-1 induced by LPS.



CP can regulate MCP-1 production, which may be associated with its effects on lung inflammation.
Zhu
Jianing
J
Pharmacy of Affiliated Sir Run Run Shaw Hospital of Zhejiang University Medical College, Hangzhou 310016, China.
Zhang
Hui
H
Xie
Qiangmin
Q
D002460:Cell Line; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D011124:Polyporaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000166:cytology*; D012333:RNA, Messenger / Q000378:metabolism
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D008070:Lipopolysaccharides; D011134:Polysaccharides; D012333:RNA, Messenger