title: Expression of the transporter encoded by the cefT gene of Acremonium chrysogenum increases cephalosporin production in Penicillium chrysogenum.
abstract: By introduction of the cefEF genes of Acremonium chrysogenum and the cmcH gene of Streptomyces clavuligerus, Penicillium chrysogenum can be reprogrammed to form adipoyl-7-amino-3-carbamoyloxymethyl-3-cephem-4-carboxylic acid (ad7-ACCCA), a carbamoylated derivate of adipoyl-7-aminodeacetoxy-cephalosporanic acid. The cefT gene of A. chrysogenum encodes a cephalosporin C transporter that belongs to the Major Facilitator Superfamily. Introduction of cefT into an ad7-ACCCA-producing P. chrysogenum strain results in an almost 2-fold increase in cephalosporin production with a concomitant decrease in penicillin by-product formation. These data suggest that cephalosporin production by recombinant P. chrysogenum strains is limited by the ability of the fungus to secrete these compounds.
authors.affiliation: Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
authors.forename: Jeroen G
authors.initials: JG
authors.lastname: Nijland
authors.forename: Andriy
authors.initials: A
authors.lastname: Kovalchuk
authors.forename: Marco A
authors.initials: MA
authors.lastname: van den Berg
authors.forename: Roel A L
authors.initials: RA
authors.lastname: Bovenberg
authors.forename: Arnold J M
authors.initials: AJ
authors.lastname: Driessen
mesh_terms: D000164:Acremonium / Q000235:genetics* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002511:Cephalosporins / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D016681:Genome, Fungal; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010406:Penicillins / Q000378:metabolism; D010408:Penicillium chrysogenum / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D014158:Transcription, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D010406:Penicillins; C025163:cephalosporin C