title: Isolation and characterization of an immunosuppressive protein from venom of the pupa-specific endoparasitoid Pteromalus puparum.
abstract: In hymenopteran parasitoids devoid of symbiotic viruses, venom proteins appear to play a major role in host immune suppression and host regulation. Not much is known about the active components of venom proteins in these parasitoids, especially those that have the functions involved in the suppression of host cellular immunity. Here, we report the isolation and characterization of a venom protein Vn.11 with 24.1 kDa in size from Pteromalus puparum, a pupa-specific endoparasitoid of Pieris rapae. The Vn.11 venom protein is isolated with the combination of ammonium sulfate precipitation and anion exchange chromatography, and its purity is verified using SDS-PAGE analysis. Like crude venom, the Vn.11 venom protein significantly inhibits the spreading behavior and encapsulation ability of host hemocytes in vitro. It is suggested that this protein is an actual component of P. puparum crude venom as host cellular-immune suppressive factor.
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
authors.forename: Ma-li
authors.initials: ML
authors.lastname: Wu
authors.forename: Gong-yin
authors.initials: GY
authors.lastname: Ye
authors.forename: Jia-ying
authors.initials: JY
authors.lastname: Zhu
authors.forename: Xue-xin
authors.initials: XX
authors.lastname: Chen
authors.forename: Cui
authors.initials: C
authors.lastname: Hu
mesh_terms: D000818:Animals; D002080:Butterflies; D004591:Electrophoresis, Polyacrylamide Gel; D006434:Hemocytes / Q000187:drug effects; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000276:immunology / Q000633:toxicity; D011679:Pupa / Q000187:drug effects; D014862:Wasp Venoms / Q000737:chemistry* / Q000276:immunology* / Q000633:toxicity; D014863:Wasps
chemical_list: D007166:Immunosuppressive Agents; D014862:Wasp Venoms