title: Anti-inflammatory activity of the extracts and fractions from Erigeron multiradiatus through bioassay-guided procedures.
abstract: BACKGROUND
  Erigeron multiradiatus (Lindl.) Benth., an herb that grows in the alpine and subalpine meadow of Qinghai-Tibet plateau, has been widely used as a folk remedy by the native people for treatment of various inflammatory ailments.
  
  
  OBJECTIVE
  In order to isolate and identify the active components of Erigeron multiradiatus for anti-inflammatory activity, a preliminary phytochemical study and a bioassay-guided fractionation and purification process was performed.
  
  
  METHODS
  The dry whole plant Erigeron multiradiatus was extracted with 50% ethanol and then separated into CHCl(3), n-BuOH, and aqueous fractions. The anti-inflammatory activities of each fraction were investigated using two in vivo inflammation models.
  
  
  RESULTS
  These results exhibited varying degrees of anti-inflammatory activities and the n-BuOH fraction showed the strongest anti-inflammatory activities. The n-BuOH fraction was then subjected to separation and purification using macroporous resins column chromatography and Sephadex LH-20 leading to two flavonoids glucuronides identified as scutellarein-7-O-beta-glucuronide and apigenin-7-O-beta-glucuronide. Furthermore, LC-MS/MS identification and quantification of isolated compounds were also performed.
  
  
  CONCLUSIONS
  Scutellarein-7-O-beta-glucuronide and apigenin-7-O-beta-glucuronide were considered as major components and principally responsible for the anti-inflammatory activity of Erigeron multiradiatus. Thus the results of our study provide a scientific basis for the utilization of Erigeron multiradiatus in traditional Tibetan medicine.
authors.affiliation: West China School of Pharmacy, Sichuan University, No. 17, Section 3, Ren-Min-Nan-Lu Road, Chengdu, Sichuan 610041, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Luo
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Xinfu
authors.initials: X
authors.lastname: Liu
authors.forename: Zhengji
authors.initials: Z
authors.lastname: Mo
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy; D049919:Erigeron / Q000737:chemistry*; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D005965:Glucuronates / Q000302:isolation & purification / Q000494:pharmacology; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D038861:Medicine, Tibetan Traditional; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018609:Tibet
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D005965:Glucuronates; D010936:Plant Extracts; C456097:apigenin-7-O-glucuronide; C484876:scutellarin; D047310:Apigenin