title: Streptococcus agalactiae DNA polymerase I is an efficient reverse transcriptase.
abstract: Genome annotations result generally from large sequence alignments by bioinformatics. Large scale biochemical data are more difficult to obtain. They derive for example from directed protein evolution experiments by selection. A previously reported directed enzyme evolution experiment by in vitro selection of Stoffel fragment variants of Taq DNA polymerase I was used here to predict the activities of Streptococcus agalactiae DNA polymerase I. The reverse transcriptase activity of S. agalactiae DNA polymerase I was measured and the kinetic parameters for this RNA-dependent DNA polymerase are given. RNA-templated DNA repair is suggested as a possible biological function for this biochemical activity.
authors.affiliation: Département de Biologie Structurale et Chimie, Institut Pasteur, Paris 15, France. fariborz.bahrami@crchul.ulaval.ca
authors.forename: Fariborz
authors.initials: F
authors.lastname: Bahrami
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Jestin
mesh_terms: D000595:Amino Acid Sequence; D004256:DNA Polymerase I / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D013292:Streptococcus agalactiae / Q000201:enzymology*
chemical_list: D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I