title: Dicamba-induced genotoxicity in Chinese hamster ovary (CHO) cells is prevented by vitamin E.
abstract: In the present study the cytogenetic and genotoxic effect of benzoic herbicide dicamba and its Argentinean commercial formulation banvel (57.71% dicamba) was evaluated and whether this effect is mediated through oxidative damage or not. The protective role of vitamin E was also studied. Sister chromatid exchange (SCE) frequency, cell-cycle progression, and cell viability analyses in CHO cells were used as in vitro end-points. Treatments with the test compounds were performed either during 24h (Protocol A) or 12h (Protocol B) before harvesting. Protocol A showed that vitamin E decreased pesticide SCE induction, corrected the cell-cycle delay and partially protected cell-death only in 500 microg/ml dicamba-treated cultures. A similar trend was found in banvel-treated cultures. Protocol B revealed similar protective role of vitamin E only for dicamba-induced geno- and cytotoxicity. Based on these observations it could be suggested that dicamba injures DNA by delivering reactive oxygen species rather than by another type of mechanism/s. Although banvel mimics the effect observed by dicamba, its formulation contains other xenobiotic/s agents able to induce cellular and DNA damage by a different mechanism/s. Further investigations are needed to acquire a comprehensive knowledge of the possible mechanism/s through dicamba and banvel exert their toxic effects.
authors.affiliation: Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: N V
authors.initials: NV
authors.lastname: González
authors.forename: S
authors.initials: S
authors.lastname: Soloneski
authors.forename: M L
authors.initials: ML
authors.lastname: Larramendy
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D019610:Cytoprotection / Q000187:drug effects*; D003996:Dicamba / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D009153:Mutagens; D014810:Vitamin E; D003996:Dicamba