Non-viral gene delivery with cationic liposome-DNA complexes.
A large amount of research activity worldwide is currently directed towards developing lipid- or polymer-based, non-viral gene vectors for therapeutic applications. This strong interest is motivated by their low toxicity, ease of production, ability to transfer large pieces of DNA into cells, and lack of immunogenic protein components. Cationic liposomes (CLs) are one of the most powerful non-viral vectors. In fact, CL-based vectors are among the prevalent synthetic carriers of nucleic acids currently used in human clinical gene therapy trials as well as in cell transfection applications for biological research. Our understanding of the mechanisms of action of CL-DNA complexes is still in its infancy. However, the relevance of a few crucial parameters, such as the lipid/DNA charge ratio (rho(chg)) and the membrane charge density of lamellar complexes (sigma(M)), is well established. To arrive at true comparisons of lipid performance, one must optimize both these parameters using a reproducible, reliable transfection assay. In this chapter, we aim to provide the reader with detailed procedures for liposome formation and transfection. It is our hope that the use of such optimized protocols will improve the comparability of transfection data obtained with novel lipids.
Ewert
Kai K
KK
Materials Department, University of California, Santa Barbara, CA, USA.
Ahmad
Ayesha
A
Bouxsein
Nathan F
NF
Evans
Heather M
HM
Safinya
Cyrus R
CR
D002412:Cations / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
D002412:Cations; D008081:Liposomes; D004247:DNA