Stereospecific analysis of sakuranetin by high-performance liquid chromatography: pharmacokinetic and botanical applications.
A stereospecific method for analysis of sakuranetin was developed. Separation was accomplished using a Chiralpak AD-RH column with UV (ultraviolet) detection at 288 nm. The stereospecific linear calibration curves ranged from 0.5 to 100 microg/mL. The mean extraction efficiency was >98%. Precision of the assay was <12% (relative standard deviation (R.S.D.)%), and within 10% at the limit of quantitation (0.5 microg/mL). Bias of the assay was lower than 10%, and within 5% at the limit of quantitation. The assay was applied successfully to pharmacokinetic quantification in rats, and the stereospecific quantification in oranges, grapefruit juice, and matico (Piper aduncum L.).
Takemoto
Jody K
JK
College of Pharmacy, Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program, Washington State University, Pullman, WA 99164-6534, USA.
Remsberg
Connie M
CM
Yáñez
Jaime A
JA
Vega-Villa
Karina R
KR
Davies
Neal M
NM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D032084:Citrus sinensis / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013237:Stereoisomerism; D035501:Uncertainty; D000094162:Phytoalexins
D005419:Flavonoids; C099724:sakuranetin; D000094162:Phytoalexins