title: [Adenovirus-mediated stromal cell-derived factor-1 alpha gene transfer promotes mesenchymal stem cell migration].
abstract: OBJECTIVE
  To explore the role of stromal-derived factor-1 (SDF-1) in the migration of mesenchymal stem cells (MSCs) and the underlying signal transduction mechanism.
  
  
  METHODS
  Rat bone marrow-derived MSCs were infected with 100 ml recombinant adenovirus containing human SDF-1alpha gene (Ad-hSDF-1alpha), and the cell migration changes were observed at 1, 2, and 3 days after the infection. Twelve hours after Ad-hSDF-1alpha transfection, the MSCs in separate cultures were treated with wortmannin (50 nmol/L), LY294002 (10 mmol/L), PD98059 (50 mmol/L), U73122 (10 mmol/L), AMD3100 (0.1 g/L), or verapamil (50 nmol/L), respectively, and the signal transduction pathways involved in MSC migration were analyzed.
  
  
  RESULTS
  The MSCs grew in colonies after transfection with Ad-hSDF-1alpha, but this growth pattern was substantially attenuated after treatment with wortmannin, LY294002, PD98059, U73122, AMD3100 and verapamil, among which U73122 and AMD3100 treatments resulted in the most conspicuous inhibitory effects.
  
  
  CONCLUSIONS
  The effect of SDF-1 in promoting MSC migration is related to mitogen-activated protein kinase, phosphatidylinositol phospholipase C, and protein kinase signal pathways.
authors.affiliation: Institute of Clinical Medicine, Shiyan People's Hospital Affiliated toYunyang Medical College, Shiyan 442000, China. yiping_wang@126.com
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Jun-ming
authors.initials: JM
authors.lastname: Tang
authors.forename: Ling-yun
authors.initials: LY
authors.lastname: Guo
authors.forename: Xia
authors.initials: X
authors.lastname: Kong
authors.forename: Jian-ye
authors.initials: JY
authors.lastname: Yang
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.forename: Yong-zhang
authors.initials: YZ
authors.lastname: Huang
authors.forename: Jia-ning
authors.initials: JN
authors.lastname: Wang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005822:Genetic Vectors / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D014162:Transfection; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D054377:Chemokine CXCL12; D004791:Enzyme Inhibitors; D020928:Mitogen-Activated Protein Kinases; D010738:Type C Phospholipases