title: Saccharomyces cerevisiae Rds2 transcription factor involvement in cell wall composition and architecture.
abstract: Although the cell wall is very important in yeasts, relatively little is known about the relationship between its structure and function. In Saccharomyces cerevisiae, a family of 55 transcription factor proteins unique to fungi, so-called zinc cluster proteins, has been described. Of these, Rds2 has been identified as an activator/inhibitor of gluconeogenesis. However, previous studies have pointed out additional roles for this protein, specifically, in the modulation of cell-wall architecture and drug sensitivity. In this work, evidence regarding the role of Rds2 as a regulator of cell-wall architecture and composition is presented based on phenotypical analysis of the cell walls prepared from a S. cerevisiae Rds2 mutant strain. Analyses of the sensitivity of this rds2Delta mutant to different drugs and to osmotic stress showed that Rds2 is indeed involved in the drug-sensitivity response and plays a role in determining osmotic sensitivity.
authors.affiliation: Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Valencia, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Moreno
authors.forename: Nunzia
authors.initials: N
authors.lastname: Tutrone
authors.forename: Rafael
authors.initials: R
authors.lastname: Sentandreu
authors.forename: Eulogio
authors.initials: E
authors.lastname: Valentín
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001557:Benzenesulfonates / Q000494:pharmacology; D002473:Cell Wall / Q000737:chemistry* / Q000187:drug effects*; D005456:Fluorescent Dyes / Q000494:pharmacology; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000187:drug effects*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D000935:Antifungal Agents; D001557:Benzenesulfonates; D005456:Fluorescent Dyes; C527549:Rds2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C007061:C.I. Fluorescent Brightening Agent 28; D004693:Glucan Endo-1,3-beta-D-Glucosidase