title: Characterization and gene expression analysis of novel matched primary and metastatic renal cell carcinoma cell lines.
abstract: Despite recent advances in molecular biology that have clarified the mechanisms involved in the metastasis of several types of cancer, the molecular mechanism underlying the metastasis of renal cell carcinoma (RCC) remains unclear. Two RCC cell lines were successfully established from the surgical specimens of a matched primary tumor and adrenal metastasis from the same RCC patient, and were designated as TMK-1P and TMK-1M, respectively. Extensive characterization was accomplished using various methods, including the Matrigel invasion assay, DNA microarray analysis and real-time reverse transcriptase (RT)-polymerase chain reaction (PCR). While TMK-1P grew faster than TMK-1M, the invasive ability of TMK-1M was higher than that of TMK-1P. DNA microarray analysis showed a large differential expression of genes related to cell adhesion and the extracellular matrix molecules of which hexabrachion (tenascin-C), epidermal growth factor receptor, cadherin-6, and beta1-catenin were down-regulated, and the 67 kDa laminin receptor 1 and transforming growth factor-beta-induced 68 kDa protein (betaig-h3) were up-regulated in TMK-1M. Real-time RT-PCR analysis confirmed this differential gene expression between the two cell lines. The RCC cell lines may be useful in studying tumor invasion and screening markers for metastasis.
authors.affiliation: Department of Urology, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo, Japan. yoshio-o@tokyo-med.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Ohno
authors.forename: Miki
authors.initials: M
authors.lastname: Izumi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tachibana
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawamura
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Yoshioka
authors.forename: Teiichiro
authors.initials: T
authors.lastname: Aoyagi
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohori
authors.forename: Kazunori
authors.initials: K
authors.lastname: Namiki
authors.forename: Noboru
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakagami
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hatano
authors.forename: Shingo
authors.initials: S
authors.lastname: Akimoto
authors.forename: Toshihide
authors.initials: T
authors.lastname: Nishimura
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007621:Karyotyping; D007680:Kidney Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D012334:RNA, Neoplasm