Diversity of virulence profiles of Shiga toxin-producing Escherichia coli serotypes in food-producing animals in Brazil.
The prevalence, serotypes and virulence profiles of Shiga toxin-producing Escherichia coli (STEC) were investigated in 205 healthy beef and dairy cattle, and 106 goats reared in the southeastern region of Minas Gerais State, Brazil. The prevalence of STEC was 57.5% (61/106) in goats, 39.2%, (40/102) in beef cattle and 17.5% (18/103) in dairy cattle. Among the 514 STEC isolates, 40 different serotypes were found and some of them were identified in a specific host. STEC isolates harboring stx1 corresponded to 15.6% (28/180), 26.7% (16/60) and 24.1% (66/274) in beef cattle, dairy cattle and goats, respectively. stx2 was found in 30% (54/180), 53.3% (32/60) and 34.7% (95/274) of beef and dairy cattle, and goats. stx1 plus stx2 sequences were harbored by 54.4% (98/180), 20% (12/60) and 41.2% (113/274) of beef cattle, dairy cattle and goats, respectively. The eae sequence was found in 15% (9/60) and 0.6% (1/180) of STEC isolates from dairy and beef cattle, respectively, and the toxB gene was found only in one O157:H7 strain isolated from beef cattle. Strains with the genetic profiles stx2 ehxA iha saa and stx1 stx2 ehxA iha saa were the most prevalent among STEC isolates from cattle. Profiles stx1 stx2 ehxA iha, stx2, and stx1 iha accounted for 75.5% (207 /274) of the STEC isolates from goats. While STEC strains carrying either stx2 alone or associated with stx1 were found more frequently in cattle, those harboring sequences stx1c and stx2d alone or associated with stx1c predominated in goats. Our data show a diversity of STEC strains in food-producing animals, most of them carrying genes linked to severe forms of human diseases.
Oliveira
Murilo Gomes
MG
Universidade Federal de Juiz de Fora, Minas Gerais, Brazil.
Brito
José Renaldi Feitosa
JR
Gomes
Tânia Aparecida Tardelli
TA
Guth
Beatriz Ernestina Cabilio
BE
Vieira
Mônica Aparecida Midolli
MA
Naves
Zita Valéria Furtado
ZV
Vaz
Tânia Mara Ibelli
TM
Irino
Kinue
K
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D004197:Disease Reservoirs / Q000662:veterinary; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D015511:Goat Diseases / Q000453:epidemiology / Q000382:microbiology*; D006041:Goats; D006801:Humans; D008297:Male; D010802:Phylogeny; D015995:Prevalence; D012703:Serotyping; D022603:Shiga Toxins / Q000096:biosynthesis / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification* / Q000302:isolation & purification* / Q000472:pathogenicity; D013045:Species Specificity; D037521:Virulence Factors / Q000235:genetics*
D022603:Shiga Toxins; D037521:Virulence Factors