title: Crystallization and preliminary X-ray analysis of the splice variant of human ankyrin repeat and suppressor of cytokine signaling box protein 9 (hASB9-2).
abstract: Human ankyrin repeat and suppressor of cytokine signaling box protein 9 (hASB9), a subunit of an Elongin C-cullin-SOCS box (ECS) E3 ubiquitin ligase complex, is believed to be involved in specific substrate-recognition for ubiquitination and degradation. In fact, this specific substrate-recognition is determined by the ankyrin repeats of hASB9 protein. Here, we have cloned and overexpressed the hASB9-2, the splice variant of hASB9 with only one ankyrin repeat domain, as a 6His-tagged recombinant protein in Escherichia coli. The purified hASB9-2 protein was crystallized by the hanging-drop vapor-diffusion technique and diffracted to 2.2A resolution. The data showed that the cubic hASB9-2 crystal belongs to space group P4(3)32 with unit-cell parameters (a=b=c=129.25A, alpha=beta=gamma=90 degrees ). An asymmetric unit in the crystal was assumed to contain one protein molecule giving the Matthews Coefficient factor of 2.81 and the solvent content of 56.3%.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, Peoples Republic of China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Fei
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Gu
authors.forename: Rui
authors.initials: R
authors.lastname: Qiu
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D012326:RNA Splicing; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D050826:Suppressor of Cytokine Signaling Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D050826:Suppressor of Cytokine Signaling Proteins