[Preparation and identification of antibody against Cecropin-XJ].
OBJECTIVE
To prepare the mouse antibody against Cecropin-XJ, identify its specificity and use it in cellular localization of Cecropin-XJ in vitro.


METHODS
The cDNA sequence of Cecropin-XJ with signal peptide sequence was subcloned into the eukaryotic expression vector pcDNA3. The recombinant plasmid was named pcDNA3-Cecropin-XJ, which was used as an antigen to immune the Kunming white mouse. Meanwhile, the cDNA sequence was subcloned into the fusion expression vector and the fusion protein was expressed as a test antigen.


RESULTS
Indirect ELISA showed that the fifth immunity's titer was highest. The immune gold-conjugated experiment showed that the prepared antibody was clearly and efficiently localized in prokaryotic cells where the Cecropin-XJ worked.


CONCLUSIONS
The antibody against Cecropin-XJ has high immune reactivity and specificity, which is beneficial to further study on Cecropin-XJ function and antibody preparation of small peptides.
Zheng
Shu-Tao
ST
Key Laboratory of Molecule Biology, College of Life Science and Technology, Xinjiang University, China. zst824@163.com
Liu
Zhong-Yuan
ZY
Wang
Hui
H
Dou
Jun
J
Zhang
Fu-Chun
FC
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D054807:Cecropins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D007074:Immunoglobulin G / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D046529:Microscopy, Electron, Transmission
D000906:Antibodies; D054807:Cecropins; D018076:DNA, Complementary; D007074:Immunoglobulin G