Immune response to lytic peptides conjugated to a betaCG fragment in treated BALB/C mice.
Hecate-betaCG and Phor14-betaCG(ala) are relatively short, amphipathic alpha-helical cationic peptides with the ability to destroy selectively breast, prostate and ovarian cancer cells. Treatment with proteins and peptides frequently initiated antibody formation. Short peptides may minimize the risk of the immune system mobilization after treatment but it is necessary to investigate whether Hecate-betaCG and Phor14-betaCG(ala) induce the immune system to produce antibody and whether they affect the reproductive organs in normal wild-type mice. The results of our experiments showed that specific antibodies, tested by the enzyme-immunoassay, were not detected in the group treated with Hecate-betaCG and Phor14-betaCG(ala). The blood concentrations of both peptides begun to decrease from 60 minutes after injection and after 240 minutes its levels were undetectable. Histopatho-logical examination exhibited degenerative changes in the prostate glands and testes in males and in the ovaries and uteri of females treated with both peptides. In conclusion, our results indicate that both relatively small and rapidly metabolized peptides are not immunogenic and can be used for further investigation as a potential cancer treatment.
Bogacki
Marek
M
Institute of Animal Reproduction and Food Research, PAS, Tuwima St. 10, Olsztyn 10-747, Poland. marbo@pan.olsztyn.pl
Enright
Frederic M
FM
Todd
William J
WJ
Hansel
William
W
D000818:Animals; D000917:Antibody Formation; D006063:Chorionic Gonadotropin / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000276:immunology; D005260:Female; D006041:Goats / Q000276:immunology; D008297:Male; D008555:Melitten / Q000031:analogs & derivatives* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology*
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments; C532487:Phor14-betaCG(ala) protein; C480619:hecate-chorionic gonadotropin beta-subunit conjugate; D008555:Melitten