eSGA: E. coli synthetic genetic array analysis.
Physical and functional interactions define the molecular organization of the cell. Genetic interactions, or epistasis, tend to occur between gene products involved in parallel pathways or interlinked biological processes. High-throughput experimental systems to examine genetic interactions on a genome-wide scale have been devised for Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans and Drosophila melanogaster, but have not been reported previously for prokaryotes. Here we describe the development of a quantitative screening procedure for monitoring bacterial genetic interactions based on conjugation of Escherichia coli deletion or hypomorphic strains to create double mutants on a genome-wide scale. The patterns of synthetic sickness and synthetic lethality (aggravating genetic interactions) we observed for certain double mutant combinations provided information about functional relationships and redundancy between pathways and enabled us to group bacterial gene products into functional modules.
Butland
Gareth
G
Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto M5S 3E1, Canada.
Babu
Mohan
M
Díaz-Mejía
J Javier
JJ
Bohdana
Fedyshyn
F
Phanse
Sadhna
S
Gold
Barbara
B
Yang
Wenhong
W
Li
Joyce
J
Gagarinova
Alla G
AG
Pogoutse
Oxana
O
Mori
Hirotada
H
Wanner
Barry L
BL
Lo
Henry
H
Wasniewski
Jas
J
Christopolous
Constantine
C
Ali
Mehrab
M
Venn
Pascal
P
Safavi-Naini
Anahita
A
Sourour
Natalie
N
Caron
Simone
S
Choi
Ja-Yeon
JY
Laigle
Ludovic
L
Nazarians-Armavil
Anaies
A
Deshpande
Avnish
A
Joe
Sarah
S
Datsenko
Kirill A
KA
Yamamoto
Natsuko
N
Andrews
Brenda J
BJ
Boone
Charles
C
Ding
Huiming
H
Sheikh
Bilal
B
Moreno-Hagelseib
Gabriel
G
Greenblatt
Jack F
JF
Emili
Andrew
A
D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D016680:Genome, Bacterial; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis