Role of SelS in lipopolysaccharide-induced inflammatory response in hepatoma HepG2 cells.
To investigate the role of SelS in bacterial lipopolysaccharide (LPS) induced inflammatory response, some parameters in LPS-stimulated HepG2 cells were comparatively studied fore-and-aft SelS silence. LPS induced the decreases of cytoplasmic glutathione peroxidase (GPx-1) mRNA expression and activity, and the increases of reactive oxygen species (ROS) level, intracellular and extracellular nitric oxide (NO) levels, inducible nitric oxide synthase (iNOS) mRNA expression and activity, and serum amyloid A1 (SAA1) mRNA expression and secreted protein level in hepatoma HepG2 cells. When SelS was suppressed by small interfering RNA (siRNA), those decreases and increases were further aggravated under LPS stimulation, respectively. In conclusion, the negative association between SelS and the LPS-induced production of ROS, NO and SAA1 demonstrated that SelS had an important role in influencing inflammatory response, and that role may be related with SelS as a central component of retro-translocation channel in endoplasmic reticulum-associated protein degradation (ERAD) and its anti-oxidative property.
Zeng
Jinhong
J
Department of Chemistry, Huazhong University of Science and Technology, 1037 Luoyu Lu, Hongshan, Wuhan, Hubei 430074, People's Republic of China.
Du
Shaoqing
S
Zhou
Jun
J
Huang
Kaixun
K
D000975:Antioxidants / Q000378:metabolism; D001692:Biological Transport; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation; D008070:Lipopolysaccharides / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D008954:Models, Biological; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D017382:Reactive Oxygen Species; D051140:Selenoproteins / Q000378:metabolism / Q000502:physiology*
D000975:Antioxidants; D008070:Lipopolysaccharides; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D051140:Selenoproteins; D052247:Nitric Oxide Synthase Type II