Identification of immunogenic cell wall-associated proteins of Streptococcus suis serotype 2.
Streptococcus suis serotype 2 (SS2) is a porcine and human pathogen with adhesive and invasive properties. The absence of suitable vaccine or virulent marker can be the bottleneck to control SS2 infection. An immunoproteome-based approach was developed to identify candidate antigens of SS2 for vaccine development. Hyperimmune sera, convalescent sera, and control sera were analyzed for reactivity by Western Blot against SS2 cell wall-associated proteins (WAPs) separated by 2-DE. A total of 34 proteins were identified by immunoproteomic analysis, of which 15 were recognized by both hyperimmune sera and convalescent sera, including most WAPs currently characterized as SS2 vaccine candidate antigens: muramidase-released protein (MRP), surface protein SP1 (Sao), and glyceraldehyde-3-phosphate dehydrogenase (GapdH). The novel immunogenic proteins may be developed as alternative antigens for further study of SS2 vaccine and diagnostics.
Zhang
Anding
A
Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Hubei, P. R. China.
Xie
Chunyan
C
Chen
Huanchun
H
Jin
Meilin
M
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D001428:Bacterial Vaccines / Q000627:therapeutic use; D002473:Cell Wall / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008856:Microscopy, Fluorescence; D013290:Streptococcal Infections / Q000517:prevention & control; D017011:Streptococcus suis / Q000737:chemistry* / Q000276:immunology; D013552:Swine
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines