Combining high-performance liquid chromatography-positron detection and on-line microdialysis for animal metabolism study of positron emission tomography probes.
Automated ultra-performance liquid chromatography (LC) with a radiometric detection system has been developed to analyze compounds labeled with short-lived positron (beta(+))-emitting radionuclides in microdialysates. This system involves (1) on-line microdialysis sampling to submit dialysates directly into the LC, (2) ultra-performance LC for improving sensitivity, resolution and speed of analysis, and (3) tandem flow-through of beta(+) and electrochemical detectors for real-time monitoring of radioactive and endogenous compounds simultaneously. This system permitted ultrasensitive measurement of radioactive compounds at the lowest detection limit of about 1Bq with a high-temporal resolution of sampling and less operator attention. The developed system was used for the continuous monitoring of L-[beta-(11)C]-3,4-dihydroxyphenylalanine (L-[beta-(11)C]DOPA) metabolites in the rat brain.
Nakao
Ryuji
R
Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. nakaor@nirs.go.jp
Okada
Maki
M
Inoue
Osamu
O
Fukumura
Toshimitsu
T
Suzuki
Kazutoshi
K
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002531:Cerebellum / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003342:Corpus Striatum / Q000378:metabolism; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D004583:Electrons; D008297:Male; D017551:Microdialysis / Q000379:methods*; D009862:Online Systems; D049268:Positron-Emission Tomography / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002250:Carbon Radioisotopes; D004295:Dihydroxyphenylalanine