title: HIV-1 Tat-mediated protein transduction of human brain creatine kinase into PC12 cells.
abstract: Epilepsy is characterized by the presence of spontaneous episodes of abnormal neuronal discharges and its pathogenic mechanisms remain poorly understood. Recently, we found that the expression of creatine kinase (CK) was markedly decreased in an epilepsy animal model using proteomic analysis. A human CK gene was fused with a HIV-1 Tat peptide to generate an in-frame Tat-CK fusion protein. The purified Tat-CK fusion protein was efficiently transduced into PC12 cells in a time- and dose-dependent manner when added exogenously to culture media. Once inside the cells, the transduced Tat-CK fusion protein was stable for 48 h. Moreover, the Tat-CK fusion protein markedly increased endogenous CK activity levels within the cells. These results suggest that Tat-CK provides a strategy for the therapeutic delivery of proteins in various human diseases including the delivery of CK for potential epilepsy treatment.
authors.affiliation: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea.
authors.forename: Min Seop
authors.initials: MS
authors.lastname: Jeong
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Lee
authors.forename: Yeom Pyo
authors.initials: YP
authors.lastname: Lee
authors.forename: So Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Lee
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Jang
authors.forename: Kil Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Oh-Shin
authors.initials: OS
authors.lastname: Kwon
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D052276:Creatine Kinase, BB Form / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016716:PC12 Cells; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis / Q000235:genetics / Q000302:isolation & purification; D014161:Transduction, Genetic / Q000379:methods*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D011993:Recombinant Fusion Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D052276:Creatine Kinase, BB Form