Proteomic profiling in an animal model of acute pancreatitis.
Acute pancreatitis (AP) is an inflammatory disease of the pancreas, which evolves in approximately 20% of the patients to a severe illness associated with a high mortality rate. In this study, we performed a comparative proteomic analysis of pancreatic tissue extracts from rats with AP and healthy rodent controls in order to identify changes in protein expression related to the pathobiological processes of this disease. Pancreatic extracts from diseased and controls rats were analyzed by 2-DE and MS/MS. A total of 125 proteins were identified from both samples. Comparative analysis allowed the detection of 42 proteins or protein fragments differentially expressed between diseased and control pancreas, some of them being newly described in AP. Interestingly, these changes were representative of the main pathobiological pathways involved in this disease. We observed activation of digestive proteases and increased expression of various inflammatory markers, including several members of the alpha-macroglobulin family. We also detected changes related to oxidative and cell stress responses. Finally, we highlighted modifications of 14-3-3 proteins that could be related to apoptosis regulation. These results showed the interest of proteomic analysis to identify changes characterizing pancreatic tissue damage and, therefore, to highlight new potential biomarkers of AP.
Fétaud
Vanessa
V
Biomedical Proteomics Research Group, Department of Bioinformatics and Structural Biology, Geneva Faculty of Medicine, Geneva, Switzerland.
Frossard
Jean-Louis
JL
Farina
Annarita
A
Pastor
Catherine M
CM
Bühler
Léo
L
Dumonceau
Jean-Marc
JM
Hadengue
Antoine
A
Hochstrasser
Denis F
DF
Lescuyer
Pierre
P
D048948:14-3-3 Proteins / Q000378:metabolism; D000208:Acute Disease; D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D014408:Biomarkers, Tumor / Q000378:metabolism; D002108:Ceruletide; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D037181:Lectins, C-Type / Q000378:metabolism; D051847:Lithostathine / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D010179:Pancreas / Q000737:chemistry; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D048948:14-3-3 Proteins; D000951:Antigens, Neoplasm; D015415:Biomarkers; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D051847:Lithostathine; D000073718:Pancreatitis-Associated Proteins; D002108:Ceruletide