Vanadate inhibits endoplasmic reticulum stress responses.
The disruption of endoplasmic reticulum function leads to an accumulation of unfolded proteins, which results in endoplasmic reticulum stress. In the present study, we investigated the effect of vanadate on such stress. Endoplasmic reticulum stress increased glucose-regulated protein 78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP) expressions in glial cell cultures. We found that vanadate inhibited the endoplasmic reticulum stress-induced increase in GRP78 and CHOP expressions at both mRNA and protein levels. Thus, these results suggest that vanadate modulates endoplasmic reticulum stress responses and that novel vanadate-responsive protein(s) might be involved in these processes.
Hosoi
Toru
T
Department of Pharmacotherapy, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Hiroshima 734-8553, Japan.
Saito
Atsushi
A
Kume
Ayaka
A
Okuma
Yasunobu
Y
Nomura
Yasuyuki
Y
Ozawa
Koichiro
K
D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004721:Endoplasmic Reticulum / Q000187:drug effects*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D018832:Molecular Chaperones / Q000096:biosynthesis; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological / Q000378:metabolism*; D051742:Transcription Factor CHOP / Q000096:biosynthesis; D014443:Tyrosine / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology*
C497250:Ddit3 protein, mouse; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D018832:Molecular Chaperones; D012333:RNA, Messenger; D051742:Transcription Factor CHOP; D014638:Vanadates; D014443:Tyrosine; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases