title: Paternity determination of captive bottlenose dolphins (Tursiops truncatus) using microsatellite DNA analysis.
abstract: We applied previously published PCR primer pairs to amplify alleles at three polymorphic microsatellite loci to determine the genetic relationship of 6 bottlenose dolphins (Tursiops truncatus) that were living together in a Japanese aquarium. The three microsatellite loci were sufficient to determine the haplotype relationships of the six dolphins, which represented three different generations. It was confirmed that this genotyping method is simple and economical for assessing, establishing and maintaining genetic diversity in captive populations and will become a very effective technique for ex situ conservation in aquariums and zoos.
authors.affiliation: Department of Molecular Life Science, School of Medicine, Tokai University, Bouseidai, Kanagawa, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sumiyama
authors.forename: Shouichi
authors.initials: S
authors.lastname: Kitamura
authors.forename: Fumio
authors.initials: F
authors.lastname: Terasawa
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hori
authors.forename: Koichi
authors.initials: K
authors.lastname: Murata
authors.forename: Jerzy K
authors.initials: JK
authors.lastname: Kulski
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Inoko
mesh_terms: D000818:Animals; D050681:Bottle-Nosed Dolphin / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000662:veterinary; D005260:Female; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D010334:Paternity; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D004247:DNA