Paenibacillus tyraminigenes sp. nov. isolated from Myeolchi-jeotgal, a traditional Korean salted and fermented anchovy.
A bacterial strain H3029, a gram-positive, rod-shaped, oxidase-negative, endospore-forming bacterium that characteristically produces tyramine from tyrosine, was isolated from a Myeolchi-jeotgal, a traditional Korean salted and fermented anchovy (Engraulis japonicus). The H3029 strain showed a high ability to produce 4140 microg/ml of tyramine from the culture broth containing 5000 microg/ml tyrosine. On the other hand, the strain produced a relatively low level of other putrefactive amines, at 973 microg/ml of putrescine and 147 microg/ml of cadaverine from the media, with each 5000 microg/ml of ornithine hydrochloride and lysine hydrochloride. The H3029 strain produced no detectable level of histamine (detection limit of 4 microg/ml) from the media containing 5000 microg/ml of histidine hydrochloride. Meanwhile, tyramine, the main product of the strain, showed the antimicrobial activity at the level of over 1 mg/disk against Staphylococcus aureus by agar diffusion test, and the mutagenicity in Ames test at 0.1 mg/plate using Salmonella typhimurium TA98 and TA1535. On the basis of the polyphasic taxonomic study, the H3029(T) strain was assigned a novel species of the genus Paenibacillus as Paenibacillus tyraminigenes sp. nov. The type strain of which is strain H3029(T) (=KCTC 10694BP(T)).
Mah
Jae-Hyung
JH
Graduate School of Biotechnology, Korea University, Seoul, Republic of Korea.
Chang
Young-Hyo
YH
Hwang
Han-Joon
HJ
D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology; D016587:Antimutagenic Agents / Q000378:metabolism / Q000494:pharmacology; D001407:Bacillus / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005516:Food Microbiology; D007723:Korea; D010802:Phylogeny; D017747:Seafood / Q000382:microbiology*; D014439:Tyramine / Q000096:biosynthesis* / Q000494:pharmacology*; D014443:Tyrosine / Q000378:metabolism
D000900:Anti-Bacterial Agents; D016587:Antimutagenic Agents; D014443:Tyrosine; D014439:Tyramine