[Effect of adenoviral vectors on the differentiation potential of human bone marrow mesenchymal stem cells].
OBJECTIVE
To investigate the effect of adenoviral vector infection on the differentiation potential of human bone marrow mesenchymal stem cells (hMSCs).


METHODS
The third-passage hMSCs were infected with the recombinant adenovirus expressing green fluorescence protein (GFP) for 2, 4, 8 and 16 days. RT-PCR was used to detect the mRNA expression of endodermal marker CYP 51, mesodermal marker SM22alpha, ectodermal marker nestin, pluripotent marker oct-4 and the alternative splicing factor nPTB. Seven days after adenovirus infection, the hMSCs were cultured in the presence of adipogenic agents for 14 days, and the adipose cells differentiated from hMSCs were detected with oil red O staining.


RESULTS
Compared with normal hMSCs, the cell infected with the adenovirus for 2, 4, 8 and 16 days showed no obvious down-regulation of CYP51, SM22alpha, nestin, OCT4 or nPTB. The hMSCs 7 days after adenovirus infection were induced to differentiate into adipose cells, with a similar differentiation rate to that of normal hMSCs. CONCLUSION The differentiation potential of hMSCs is not affected by adenovirus infection, suggesting that adenovirus can be used as the gene delivery vector in MSC differentiation studies.
Shan
Zhi-xin
ZX
Research Center of Medical Sciences, Guangdong Provincial People's Hospital/Guangdong Provincial Cardiovascular Diseases Institute, Guangzhou 510080, China. zhixinshan@yahoo.com.cn
Lin
Qiu-xiong
QX
Li
Xiao-hong
XH
Deng
Chun-yu
CY
Zhou
Zhi-ling
ZL
Huang
Wei
W
Huang
Xiao-zhong
XZ
Yu
Xi-yong
XY
D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D054884:Host-Pathogen Interactions; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D049452:Green Fluorescent Proteins