title: Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri.
abstract: Peroxiredoxin V (PRX V) is known as an atypical 2-cysteine peroxiredoxin that protects the organisms against various oxidative stresses and functions in signal transduction. The cDNA of a PRX V gene (designated as CfPRX) was cloned from scallop Chlamys farreri. The full-length sequence of CfPRX cDNA was of 2,179 bp with a 564 bp open reading frame encoding a peptide of 187 amino acids. Sequence comparison showed that CfPRX shared higher identities with PRX Vs than that with other isoforms of PRX, indicating CfPRX was a member of the PRX V family. Fluorescent real-time quantitative PCR analysis revealed the presence of CfPRX transcripts in gill filaments, adductor muscle, heart, gonad, kidney and hemocytes, and the stimulation of Listonella anguillarum significantly (P < 0.01) enhanced the mRNA expression of CfPRX in hemocyte. These results indicated that CfPRX was a constitutive and inducible acute-phase protein which was involved in the immune resistance to L. anguillarum stimulation.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd, Qingdao, 266071, Shandong, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Cong
authors.forename: Duojiao
authors.initials: D
authors.lastname: Ni
authors.forename: Linsheng
authors.initials: L
authors.lastname: Song
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhao
authors.forename: Limei
authors.initials: L
authors.lastname: Qiu
authors.forename: Ling
authors.initials: L
authors.lastname: Li
mesh_terms: D000209:Acute-Phase Proteins; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006434:Hemocytes; D007109:Immunity / Q000235:genetics; D049897:Pectinidae / Q000235:genetics* / Q000276:immunology; D054464:Peroxiredoxins / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger; D014018:Tissue Distribution
chemical_list: D000209:Acute-Phase Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D054464:Peroxiredoxins