title: Spatially well-defined binary brushes of poly(ethylene glycol)s for micropatterning of active proteins on anti-fouling surfaces.
abstract: We report a novel method for micropatterning of active proteins on anti-fouling surfaces via spatially well-defined and dense binary poly(ethylene glycol)s (PEGs) brushes with controllable protein-docking sites. Binary brushes of poly(poly(ethylene glycol) methacrylate-co-poly(ethylene glycol)methyl ether methacrylate), or P(PEGMA-co-PEGMEMA), and poly(poly(ethylene glycol)methyl ether methacrylate), or P(PEGMEMA), were prepared via consecutive surface-initiated atom transfer radical polymerizations (SI-ATRPs) from a resist-micropatterned Si(100) wafer surface. The terminal hydroxyl groups on the side chains of PEGMA units in the P(PEGMA-co-PEGMEMA) microdomains were activated directly by 1,1'-carbonyldiimidazole (CDI) for the covalent coupling of human immunoglobulin (IgG) (as a model active protein). The resulting IgG-coupled PEG microdomains interact only and specifically with target anti-IgG, while the other PEG microregions effectively prevent specific and non-specific protein fouling. When extended to other active biomolecules, microarrays for specific and non-specific analyte interactions with a high signal-to-noise ratio could be readily tailored.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore 119260, Singapore.
authors.forename: F J
authors.initials: FJ
authors.lastname: Xu
authors.forename: H Z
authors.initials: HZ
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: Y H Eric
authors.initials: YH
authors.lastname: Teo
authors.forename: C X
authors.initials: CX
authors.lastname: Zhu
authors.forename: E T
authors.initials: ET
authors.lastname: Kang
authors.forename: K G
authors.initials: KG
authors.lastname: Neoh
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties
chemical_list: D007074:Immunoglobulin G; D011092:Polyethylene Glycols