Reproducible comet assay of amorphous silica nanoparticles detects no genotoxicity.
Genotoxicity of commercial colloidal and laboratory-synthesized silica nanoparticles was tested using the single cell gel electrophoresis or Comet assay. By using a carefully developed protocol and careful characterization of the nanoparticle dispersions, Comet assays were performed on 3T3-L1 fibroblasts with 3, 6, and 24 h incubations and 4 or 40 microg/ml of silica nanoparticles. No significant genotoxicity was observed for the nanoparticles tested under the conditions described, and results were independently validated in two separate laboratories, showing that in vitro toxicity testing can be quantitatively reproducible.
Barnes
Clifford A
CA
University of Ulster, Coleraine, United Kingdom.
Elsaesser
Andreas
A
Arkusz
Joanna
J
Smok
Anna
A
Palus
Jadwiga
J
Leśniak
Anna
A
Salvati
Anna
A
Hanrahan
John P
JP
Jong
Wim H de
WH
Dziubałtowska
Elzbieta
E
Stepnik
Maciej
M
Rydzyński
Konrad
K
McKerr
George
G
Lynch
Iseult
I
Dawson
Kenneth A
KA
Howard
C Vyvyan
CV
D020552:Comet Assay; D046529:Microscopy, Electron, Transmission; D009152:Mutagenicity Tests; D053758:Nanoparticles; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*
D012822:Silicon Dioxide