The stable, functional core of DdrA from Deinococcus radiodurans R1 does not restore radioresistance in vivo.
DdrA protein binds to and protects 3' DNA ends and is essential for preserving the genome integrity of Deinococcus radiodurans following treatment by gamma radiation in an environment lacking nutrients. Limited proteolysis was used to identify a stable and functional protein core, designated DdrA157, consisting of the first 157 residues of the protein. In vitro, the biochemical differences between wild-type and mutant proteins were modest. DdrA exhibits a strong bias in binding DNA with 3' extensions but not with 5' extensions. The mutant DdrA157 exhibited a greater affinity for 5' DNA ends but still bound to 3' ends more readily. However, when we replaced the wild-type ddrA gene with the mutant gene for ddrA157, the resulting D. radiodurans strain became almost as sensitive to gamma radiation as the ddrA knockout strain. These results suggest that while the stable protein core DdrA157 is functional for DNA binding and protection assays in vitro, the carboxyl terminus is required for important functions in vivo. The C terminus may therefore be required for protein or DNA interactions or possibly as a regulatory region for DNA binding or activities not yet identified.
Harris
Dennis R
DR
Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Madison, WI, USA.
Ngo
Khanh V
KV
Cox
Michael M
MM
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015153:Blotting, Western; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D034301:Deinococcus / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D024202:Electrophoretic Mobility Shift Assay; D005720:Gamma Rays; D009154:Mutation; D011485:Protein Binding
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins