title: [Effect of adenoviral vectors on the differentiation potential of human bone marrow mesenchymal stem cells].
abstract: OBJECTIVE
  To investigate the effect of adenoviral vector infection on the differentiation potential of human bone marrow mesenchymal stem cells (hMSCs).
  
  
  METHODS
  The third-passage hMSCs were infected with the recombinant adenovirus expressing green fluorescence protein (GFP) for 2, 4, 8 and 16 days. RT-PCR was used to detect the mRNA expression of endodermal marker CYP 51, mesodermal marker SM22alpha, ectodermal marker nestin, pluripotent marker oct-4 and the alternative splicing factor nPTB. Seven days after adenovirus infection, the hMSCs were cultured in the presence of adipogenic agents for 14 days, and the adipose cells differentiated from hMSCs were detected with oil red O staining.
  
  
  RESULTS
  Compared with normal hMSCs, the cell infected with the adenovirus for 2, 4, 8 and 16 days showed no obvious down-regulation of CYP51, SM22alpha, nestin, OCT4 or nPTB. The hMSCs 7 days after adenovirus infection were induced to differentiate into adipose cells, with a similar differentiation rate to that of normal hMSCs. CONCLUSION The differentiation potential of hMSCs is not affected by adenovirus infection, suggesting that adenovirus can be used as the gene delivery vector in MSC differentiation studies.
authors.affiliation: Research Center of Medical Sciences, Guangdong Provincial People's Hospital/Guangdong Provincial Cardiovascular Diseases Institute, Guangzhou 510080, China. zhixinshan@yahoo.com.cn
authors.forename: Zhi-xin
authors.initials: ZX
authors.lastname: Shan
authors.forename: Qiu-xiong
authors.initials: QX
authors.lastname: Lin
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Li
authors.forename: Chun-yu
authors.initials: CY
authors.lastname: Deng
authors.forename: Zhi-ling
authors.initials: ZL
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Xiao-zhong
authors.initials: XZ
authors.lastname: Huang
authors.forename: Xi-yong
authors.initials: XY
authors.lastname: Yu
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D054884:Host-Pathogen Interactions; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D049452:Green Fluorescent Proteins