Estradiol and progesterone differentially regulate the dendritic arbor of neurons in the hypothalamic ventromedial nucleus of the female rat (Rattus norvegicus).
The ventromedial nucleus of the hypothalamus (VMH), with its major subdivisions, the dorsomedial and ventrolateral VMH (dmVMH and vlVMH, respectively), has been studied extensively for its role in female sexual behavior. This behavior is controlled by the vlVMH through the cellular actions of estradiol combined with progesterone. Although the effects of treatment with estradiol alone on neuronal morphology in the vlVMH have been examined, much less is known about the combined effects of estradiol and progesterone on neuronal structure. The present study employed Golgi impregnation to investigate the effects of estradiol treatment alone vs. estradiol combined with progesterone treatment on dendritic arbor of VMH neurons. The dendritic arbor of VMH neurons was somewhat different in the vlVMH vs. the dmVMH, with longer and more dendrites in the vlVMH. Estradiol treatment alone caused a marked reduction in the length of long primary dendrites in the vlVMH, but not in the dmVMH. The estradiol-induced retraction of long primary dendrites in the vlVMH was reversed within 4 hours of progesterone treatment. The differences in the dendritic arbors of dmVMH and vlVMH provide further support for the notion that these two regions have different patterns of neural connectivity. In addition, this study is the first to report opposing effects of estradiol alone vs. estradiol plus progesterone on the dendritic arbor of neurons in the vlVMH. These results suggest a structural mechanism for estradiol alone to have a modest effect on mating behavior while setting the stage for its ample expression.
Griffin
Gerald D
GD
Neuroscience Graduate Group, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. gdg@mail.med.upenn.edu
Flanagan-Cato
Loretta M
LM
D000818:Animals; D048430:Cell Shape; D003712:Dendrites / Q000187:drug effects / Q000648:ultrastructure; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012726:Sexual Behavior, Animal / Q000187:drug effects; D014697:Ventromedial Hypothalamic Nucleus / Q000166:cytology* / Q000187:drug effects
D011374:Progesterone; D004958:Estradiol