title: Towards microfabricated biohybrid artificial lung modules for chronic respiratory support.
abstract: We have utilized soft lithography techniques to create three-dimensional arrays of blood microchannels and gas pathways in poly(dimethylsiloxane) (PDMS) that approach the microvascular scale of the natural lung. The blood microchannels were lined with endothelial cells in an effort to provide a non-thrombogenic surface that might ultimately reduce the need for systemic anticoagulation. A novel design and fabrication method were developed to create prototype modules for gas permeance and cell culture testing. The gas permeance modules contained 6 layers, four gas and two blood, while the modules for cell culture testing contained two layers of blood channels. The gas permeance of the modules was examined and maximum values of 9.16 x 10(-6) and 3.55 x 10(-5) mL/s/cm(2)/cmHg, for O(2) and CO(2) respectively, were obtained. Finally, endothelial cells were seeded and dynamically cultured in prototype cell culture modules. Confluent and viable cell monolayers were achieved after 10 days of perfusion.
authors.affiliation: Medical Devices Laboratory, 215 McGowan Institute for Regenerative Medicine, University of Pittsburgh, 3025 East Carson St., Pittsburgh, PA, 15203, USA.
authors.forename: Kristie A
authors.initials: KA
authors.lastname: Burgess
authors.forename: Hsin-Hua
authors.initials: HH
authors.lastname: Hu
authors.forename: William R
authors.initials: WR
authors.lastname: Wagner
authors.forename: William J
authors.initials: WJ
authors.lastname: Federspiel
mesh_terms: D001187:Artificial Organs; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D008168:Lung; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D010101:Oxygen Consumption / Q000502:physiology