The in vitro anti-osteoporotic activity of some diarylheptanoids and lignans from the rhizomes of Dioscorea spongiosa.
Three diarylheptanoids and three lignans from the 90 % ethanol fraction of the rhizomes of DIOSCOREA SPONGIOSA were isolated and subjected to IN VITRO experiments to investigate their activity on osteoblasts and osteoclasts. Diarylheptanoid 1 and furofuran lignan 6 were found to significantly stimulate the proliferation of primary osteoblasts and 6 also mildly stimulated osteoblast mineralization by mouse calvaria osteoblastic cells. All diarylheptanoids and lignans at a concentration of 200 muM completely inhibited the formation of osteoclast-like cells. These results demonstrate that the isolated diarylheptanoids and lignans in the 90 % ethanol fraction have significant anti-osteoporotic activities.
Yin
Jun
J
Department of Pharmacognosy, Shenyang Pharmaceutical University, Shenyang, PR China. yinjun2002@yahoo.com
Han
Na
N
Liu
Zhihui
Z
Xu
Xinyang
X
Zhang
Baoyan
B
Kadota
Shigetoshi
S
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D036381:Diarylheptanoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027742:Dioscorea / Q000737:chemistry*; D005260:Female; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D010010:Osteoclasts / Q000187:drug effects*; D027343:Rhizome / Q000737:chemistry*
D036381:Diarylheptanoids; D017705:Lignans