A transcutaneous vaccination system using a hydrogel patch for viral and bacterial infection.
One of the most important anthropic missions is preventing the global spread of infectious diseases. Vaccination is the only available preventive treatment for infectious diseases, but the availability of vaccines in developing countries is not adequate. We report a simple, easy-to-use, noninvasive hydrogel patch transcutaneous vaccination system. Antigen (Ag)-specific IgG production was induced by applying an Ag-immersed patch to non-pretreated mouse auricle or hairless rat back skin. Immunofluorescence histochemical analysis revealed that Langerhans cells resident in the epidermal layer captured the antigenic proteins delivered by the hydrogel patch, which promoted the penetration of antigenic proteins through the stratum corneum, and that Ag-capturing Langerhans cells migrated into draining lymph nodes. Humoral immunity elicited by our transcutaneous vaccination system demonstrated neutralizing activity in both adenoviral infection and passive-challenge tetanus toxin experiments. The use of this hydrogel patch transcutaneous vaccination system will facilitate the global distribution of effective and convenient vaccines.
Ishii
Yumiko
Y
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Nakae
Tomoko
T
Sakamoto
Fumiko
F
Matsuo
Kazuhiko
K
Matsuo
Keisuke
K
Quan
Ying-Shu
YS
Kamiyama
Fumio
F
Fujita
Takuya
T
Yamamoto
Akira
A
Nakagawa
Shinsaku
S
Okada
Naoki
N
D000279:Administration, Cutaneous; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000956:Antigens, Viral / Q000008:administration & dosage; D001424:Bacterial Infections / Q000276:immunology / Q000517:prevention & control*; D036022:Capsid Proteins / Q000008:administration & dosage / Q000493:pharmacokinetics; D002460:Cell Line; D004337:Drug Carriers / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage*; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D051381:Rats; D054772:Rats, Hairless; D012869:Skin Absorption; D013745:Tetanus Toxoid / Q000008:administration & dosage / Q000493:pharmacokinetics; D014611:Vaccination / Q000379:methods*; D014612:Vaccines / Q000008:administration & dosage* / Q000493:pharmacokinetics; D014777:Virus Diseases / Q000276:immunology / Q000517:prevention & control*
D000956:Antigens, Viral; D036022:Capsid Proteins; D004337:Drug Carriers; D007074:Immunoglobulin G; D013745:Tetanus Toxoid; D014612:Vaccines; C018315:hexon capsid protein, Adenovirus; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate