Glycan arrays on aluminum-coated glass slides.
We have developed a novel method of immobilizing glycans onto aluminum-coated glass (ACG) slides for potential use in disease diagnosis and drug discovery. The quality of these sugar chips can be assessed by mass spectrometry and fluorescence measurements with high sensitivity. The unique properties of ACG slides include: 1) the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; 2) the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and 3) the slides are more sensitive than transparent glass slides in binding analysis. To demonstrate this, we synthesized a model compound of mannose with a built-in photocleavable linker bound to the ACG slide surface. The molecular weight of the grafted mannose was identified by mass spectrometry, and the slide was subjected to biotinylated ConA binding followed by Cy3-tagged streptavidin detection. This method was further extended to the preparation of glycan arrays containing lactose and the cancer antigen Globo H.
Tseng
Susan Y
SY
Genomics Research Center, Academia Sinica, Taiwan.
Wang
Cheng-Chi
CC
Lin
Chin-Wei
CW
Chen
Cheng-Lung
CL
Yu
Wen-Yueh
WY
Chen
Chung-Hsuan
CH
Wu
Chung-Yi
CY
Wong
Chi-Huey
CH
D000535:Aluminum / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry; D013058:Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010777:Photochemistry; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013057:Spectrum Analysis; D013499:Surface Properties
D003432:Cross-Linking Reagents; D011134:Polysaccharides; D011506:Proteins; D000535:Aluminum; D008358:Mannose