Protein modification for single molecule fluorescence microscopy.
Single molecule methods have emerged as a powerful new tool for exploring biological phenomena. We provide a brief overview of the scope of current experiments and assess the limitations of both fluorescent labels and the means to achieve protein modification for single molecule microscopy.
Dillingham
Mark S
MS
DNA-protein Interactions Unit, Department of Biochemistry, University of Bristol, Bristol, UKBS8 1TD. mark.dillingham@bristol.ac.uk
Wallace
Mark I
MI
D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D008856:Microscopy, Fluorescence; D015335:Molecular Probes; D036103:Nanotechnology; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry*
D005456:Fluorescent Dyes; D015335:Molecular Probes; D011506:Proteins; D049452:Green Fluorescent Proteins