title: Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study.
abstract: Signaling lymphocyte activation molecule (SLAM) is one of the receptors for canine distemper virus (CDV). In this study, canine and feline cells expressing canine SLAM, designated A-72/cSLAM and CRFK/cSLAM, were established for the in vitro study of canine distemper. Recent CDV isolates, KDK-1 and 246, which belong to genotypes Asia/H1 and Asia/H2, respectively, rapidly grew and produced distinct syncytia in both the SLAM-expressing cells. The virus-neutralizing (VN) test was successfully performed using these cells, and the results indicated that sera from dogs experimentally infected with KDK-1 had higher VN titers for homologous strain KDK-1 than for heterologous strain 246 and the vaccine Onderstepoort. These newly established cells expressing canine SLAM would help virological and serological analyses of canine distemper.
authors.affiliation: Laboratory of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nakano
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kameo
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Andoh
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ohno
authors.forename: Masami
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism / Q000502:physiology; D002415:Cats; D004217:Distemper Virus, Canine / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D004285:Dogs; D005838:Genotype; D008213:Lymphocyte Activation; D009500:Neutralization Tests / Q000662:veterinary; D011956:Receptors, Cell Surface / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014776:Virus Cultivation / Q000379:methods / Q000662:veterinary*
chemical_list: D015703:Antigens, CD; D011956:Receptors, Cell Surface; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1