title: The use of electrochemically activated saline as a uterine instillation in pony mares.
abstract: Twelve pony mares were randomly assigned to either a control or a treatment group and inseminated with fresh, raw semen from a single stallion of known fertility in a cross-over trial design. Pregnancy was diagnosed by transrectal ultrasound 12-14 days post-ovulation and then terminated by administration of a luteolytic dose of cloprostenol. Treatment mares received a uterine instillation of 100 ml of electrochemically activated (ECA) saline 4-12 hours post-insemination. Control mares received no treatment post-insemination. Per cycle pregnancy rate was 58.3 % in the control group and 50 % in the treatment group. There was no statistical difference (P = 1.000) in pregnancy rate between the 2 groups. The principles of ECA and applications of ECA saline are discussed.
authors.affiliation: Section of Reproduction, Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110 South Africa. henry.annandale@up.ac.za
authors.forename: C H
authors.initials: CH
authors.lastname: Annandale
authors.forename: M L
authors.initials: ML
authors.lastname: Schulman
authors.forename: R D
authors.initials: RD
authors.lastname: Kirkpatrick
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D018592:Cross-Over Studies; D005260:Female; D006736:Horses / Q000502:physiology*; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D008297:Male; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary; D011247:Pregnancy; D018873:Pregnancy Rate; D011897:Random Allocation; D012965:Sodium Chloride; D013076:Sperm Count / Q000662:veterinary
chemical_list: D006063:Chorionic Gonadotropin; D012965:Sodium Chloride