HPLC-MS/MS determination of a hardly soluble drug in human urine through drug-albumin binding assisted dissolution.
ABT-263 is under development for treatment of cancer. In order to support clinical trials, an analytical method for ABT-263 quantification in human urine became necessary. Due to the extremely poor solubility of ABT-263 in aqueous and most common organic solvents, a critical step was to dissolve the drug into urine matrix. Although other potential approaches could be used, addition of powder albumin was found to be the most advantageous. Albumin powder does not significantly alter urine sample volume (< or = 2.8%) and a range of albumin to urine sample volume ratios can be allowed for full recovery of drug and thus accurate quantification. The procedure is fairly simple and can potentially be a universal approach for compounds with low solubility in urine, but strong protein binding. The method has been validated to support clinical trials.
Rodila
Ramona
R
Department of Drug Analysis, Abbott Laboratories, Abbott Park, IL 60064, USA.
Kim
Grace E
GE
Fan
Leimin
L
Chang
Min S
MS
Zhang
Jun
J
Wu
Huaiqin
H
El-Shourbagy
Tawakol A
TA
D000418:Albumins / Q000378:metabolism*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000418:Albumins