[Mechanism of rat sciatic nerve regeneration induced by human hair keratin].
OBJECTIVE
To evaluate the effect of human hair keratin (HHK) in peripheral nerve repair and explore the mechanism of sciatic nerve regeneration.


METHODS
Rat models of sciatic nerve damage was established by creating a 10-mm gap in the sciatic nerve, which was bridged with a HHK implant. Histological examinations of the nerve tissues were performed at different time points after the surgery.


RESULTS
During the period from 2 days to 2 weeks following HHK implantation, Schwann cells were found to undergo dedifferentiation and proliferate along the HHK implant. Three weeks after HHK implantation, numerous macrophages and megakaryocytes occurred around the HHK, and a large quantity of regenerated Schwann cells aligned in orderly fashion was seen between the fine filaments of partially degraded HHK, where axons and capillaries were also observed. Six weeks later, massive nerve fibers and capillaries developed around the HHK, and at 9 weeks, the HHK implant was substantially degraded and numerous regenerated nerve fibers occurred characterized by obvious epineurium and perineurium. Till 12 weeks after HHK implantation, HHK was almost completely degraded and replaced by the newly regenerated nerve fibers that had grown across the nerve defect.


CONCLUSIONS
HHK is an ideal material for nerve injury repair. Apocytosis plays a key role in the differentiation process of highly differentiated Schwann cells into immature Schwann cells following nerve injury. As a protective mechanism, the axons undergo enclosure and dissociation following injuries, and the intact axons give rise to growth cones that extend fibers of growing buds to competitively bind the one or more Schwann cells, but only one such but finally develops into a complete axon. The nerve fiber barrier membrane is derived from the capillary menchymal stem cells and the outmost vascular barrier membrane. The regeneration of the Schwann cells, axons and the nerve membrane is the result of self-organization through a well synchronized and coordinated mechanism.
Hu
Lian-mei
LM
Key Laboratory of Hepatopathy, 458 Hospital of PLA, and Institute of Comparative Medicine, Southern Medical University, Guangzhou 510602, China. hulianmei@yahoo.com.cn
Piao
Zhong-xian
ZX
Wang
Qi-wei
QW
Wang
Wan-shan
WS
Gu
Wei-wang
WW
Piao
Ying-jie
YJ
D000818:Animals; D005260:Female; D006197:Hair / Q000737:chemistry; D006801:Humans; D007633:Keratins / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D019736:Prostheses and Implants; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000503:physiopathology*
D007633:Keratins