Rubellimicrobium mesophilum sp. nov., a mesophilic, pigmented bacterium isolated from soil.
A pink- to light-reddish-pigmented, aerobic, pointed, budding, mesophilic strain designated MSL-20T was isolated from soil from Bigeum island, Korea. The isolate was Gram-negative. Cells were motile rods without flagella. The temperature and pH for growth were 20-37 degrees C (optimum 28 degrees C) and pH 7.0-11.0 (optimum pH 7.0+/-0.2). Q-10 was the predominant respiratory ubiquinone. The major fatty acids were C16:0 (37 %), C18: 1 omega7c (36 %) and 11-methyl C18:1 omega7c (12 %). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that Rubellimicrobium thermophilum DSM 16684T was its closest neighbour (95.2 % similarity); the isolate had less than 97.0 % similarity to all species with validly published names. Chemotaxonomic data were determined for this isolate, including peptidoglycan type, diagnostic sugar composition, fatty acid profiles, ubiquinones and polar lipids; the results supported the affiliation of strain MSL-20T to the genus Rubellimicrobium. The DNA G+C content was 72.3 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain MSL-20(T) represents a novel species of the genus Rubellimicrobium, for which the name Rubellimicrobium mesophilum sp. nov. is proposed. The type strain is MSL-20T (=KCTC 22012T =DSM 19309T).
Dastager
Syed G
SG
Functional Metabolomics Research Center, KRIBB, Daejeon, Republic of Korea.
Lee
Jae-Chan
JC
Ju
Yoon-Jung
YJ
Park
Dong-Jin
DJ
Kim
Chang-Jin
CJ
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S