title: Tetrandrine ameliorates dextran-sulfate-sodium-induced colitis in mice through inhibition of nuclear factor -kappaB activation.
abstract: BACKGROUND
  Activation of nuclear factor (NF)-kappaB has been shown to play a critical role in the pathogenesis of ulcerative colitis (UC), and tetrandrine, a bisbenzylisoquinoline alkaloid isolated from the Chinese herb Radix Stephania tetrandra, has been demonstrated to be a potent inhibitor of NF-kappaB activation. The purpose of the study was to investigate effects of tetrandrine on experimental model of UC.
  
  
  METHODS
  Tetrandrine was administered in experimental colitis induced by dextran sulfate sodium (DSS). The disease activity index (DAI) and histological score were observed. NF-kappaB DNA binding activity was assessed by electrophoretic mobility shift assay. The expression of tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta were measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay.
  
  
  RESULTS
  A significant improvement was observed in DAI and histological score in mice with tetrandrine, and the increase in NF-kappaB DNA binding activity, myeloperoxidase activity, IL-1beta, and TNF-alpha in mice with DSS-induced colitis was significantly reduced following administration of tetrandrine.
  
  
  CONCLUSIONS
  The administration of tetrandrine leads to an amelioration of DSS-induced colitis, suggesting administration of tetrandrine may provide a therapeutic approach for UC.
authors.affiliation: Department of Geriatric Medicine and Gastroenterology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Dei Kui
authors.initials: DK
authors.lastname: Zhang
authors.forename: Li Na
authors.initials: LN
authors.lastname: Cheng
authors.forename: Xiao Li
authors.initials: XL
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Jun Ying
authors.initials: JY
authors.lastname: Xiang
authors.forename: Hua Tian
authors.initials: HT
authors.lastname: Gan
mesh_terms: D000818:Animals; D044182:Benzylisoquinolines / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D016264:Dextran Sulfate / Q000009:adverse effects; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism
chemical_list: D044182:Benzylisoquinolines; D007166:Immunosuppressive Agents; D053583:Interleukin-1beta; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C009438:tetrandrine; D016264:Dextran Sulfate; D009195:Peroxidase