title: Peptide-grafted nanodiamonds: preparation, cytotoxicity and uptake in cells.
abstract: Nanodiamonds that were prepared by high pressure/high temperature were functionalized with biomolecules for biological applications. Nanodiamonds (NDs, < or =35 nm) that were coated by silanization or with polyelectrolyte layers were grafted with a fluorescent thiolated peptide via a maleimido function; this led to an aqueous colloidal suspension that was stable for months. These substituted NDs were not cytotoxic for CHO cells. Their capacity to enter mammalian cells, and their localisation inside were ascertained after labelling the nucleus and actin, by examining the cells by confocal, reflected light and fluorescence microscopy.
authors.affiliation: UPMC Université Paris 06, CNRS-UMR 7613, Synthèse, Structure et Fonction de Molécules Bioactives case courrier 182, 4 Place Jussieu, 75005 Paris, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Vial
authors.forename: Christelle
authors.initials: C
authors.lastname: Mansuy
authors.forename: Sandrine
authors.initials: S
authors.lastname: Sagan
authors.forename: Theano
authors.initials: T
authors.lastname: Irinopoulou
authors.forename: Fabienne
authors.initials: F
authors.lastname: Burlina
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Boudou
authors.forename: Gérard
authors.initials: G
authors.lastname: Chassaing
authors.forename: Solange
authors.initials: S
authors.lastname: Lavielle
mesh_terms: D000818:Animals; D016466:CHO Cells; D016923:Cell Death / Q000187:drug effects; D002477:Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D018130:Diamond / Q000378:metabolism* / Q000633:toxicity*; D042541:Intracellular Space / Q000187:drug effects / Q000201:enzymology; D053758:Nanoparticles / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D010088:Oxidoreductases / Q000378:metabolism
chemical_list: D009842:Oligopeptides; D018130:Diamond; D010088:Oxidoreductases