Changes of endothelin-1 expression in cerebral basilar arteries of scald rats.
The purpose of this study was to investigate the influences of scald on endothelin-1 (ET-1) expression in cerebral vessels of rats, and to further elucidate the relationship between changes of ET-1 expression and scald-induced cerebral vascular remodelling resulting in cerebral vasospasm. The ET-1 levels in the cerebral basilar arteries were estimated by radioimmunoassay. Expression of ET-1 and its mRNA were determined by Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. Changes of cerebral basilar artery histology were observed and diameters were measured by angiography. The results showed that the ET-1 levels at 3h after scald were enhanced, peaking at 12h; the expression of ET-1 mRNA was also enhanced, peaking at 6h. Simultaneous vascular remodelling was observed in the cerebral basilar arteries, vasoconstriction peaking at 12h. It is suggested that ET-1 plays an important role in the pathogenesis of scald-induced cerebral vasospasm and cerebral circulation disorder.
Lin
Xuequn
X
Institute of Cerebral Vessels Research, Department of Anatomy, Medical College of Nanchang University, Jiangxi, PR China. linxq56@163.com
Dai
Yucheng
Y
Zhu
Gaochun
G
Wan
Lidan
L
D000818:Animals; D001488:Basilar Artery / Q000378:metabolism* / Q000473:pathology; D015153:Blotting, Western; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D002560:Cerebrovascular Circulation / Q000502:physiology; D019332:Endothelin-1 / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020301:Vasospasm, Intracranial / Q000378:metabolism* / Q000473:pathology
D019332:Endothelin-1