title: MYMIV replication initiator protein (Rep): roles at the initiation and elongation steps of MYMIV DNA replication.
abstract: In order to explore the mechanism of geminivirus DNA replication, we show that the Replication initiator (Rep) protein encoded by Mungbean yellow mosaic India virus (MYMIV), a member of the family Geminiviridae, binds specifically to the iterons present in the viral DNA replication origin (CR-A) in a highly ordered manner that might be a prerequisite for the initiation of replication. MYMIV Rep also acts as a helicase during the post-initiation stage and is upregulated in presence of the RPA32 subunit of Replication Protein A. The implication of these findings on the initiation and elongation stages of MYMIV DNA replication has been discussed.
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Dharmendra Kumar
authors.initials: DK
authors.lastname: Singh
authors.forename: Punjab Singh
authors.initials: PS
authors.lastname: Malik
authors.forename: Nirupam Roy
authors.initials: NR
authors.lastname: Choudhury
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Mukherjee
mesh_terms: D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication / Q000502:physiology*; D004279:DNA, Viral / Q000096:biosynthesis; D017791:Geminiviridae / Q000235:genetics / Q000502:physiology*; D018741:Replication Origin / Q000502:physiology*; D051716:Replication Protein A / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014779:Virus Replication / Q000235:genetics
chemical_list: D004279:DNA, Viral; D051716:Replication Protein A; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases