title: Vimentin is required for dengue virus serotype 2 infection but microtubules are not necessary for this process.
abstract: The present study investigated the effect of microtubules (MTs) and vimentin during dengue virus serotype 2 (DV2) infection. Immunostaining showed that DV2 infection induced MT and vimentin reorganization. Colocalization of DV2 antigens with MTs or vimentin were often observed in ECV304 cells. MT-disrupting agents could enhance DV2 release but did not affect other steps of virus replication. In contrast, disruption of vimentin inhibited DV2 infection. Our results suggest that an MT-dependent mechanism may not be necessary for DV2 infection, and MT disruption may promote DV2 release. However, vimentin is required for DV2 infection.
authors.affiliation: Department of Microbiology, Third Military Medical University, 400038 Chongqing, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Na
authors.initials: N
authors.lastname: Gao
authors.forename: Jia-li
authors.initials: JL
authors.lastname: Wang
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Tian
authors.forename: Zong-tao
authors.initials: ZT
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: An
mesh_terms: D002460:Cell Line; D003715:Dengue / Q000378:metabolism* / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D006801:Humans; D008870:Microtubules / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D014746:Vimentin / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D014746:Vimentin