title: Sleep-wakefulness effects after microinjections of hypocretin 1 (orexin A) in cholinoceptive areas of the cat oral pontine tegmentum.
abstract: Hypocretinergic/orexinergic neurons, which are known to be implicated in narcolepsy, project to the pontine tegmentum areas involved in the control of rapid eye movement (REM) sleep. Here, we report the effects on sleep-wakefulness produced by low-volume microinjections of hypocretin (Hcrt)1 (20-30 nL, 100, 500 and 1000 microm) and carbachol (20-30 nL, 0.1 m) delivered in two areas of the oral pontine tegmentum of free-moving cats with electrodes for chronic sleep recordings: in the dorsal oral pontine tegmentum (DOPT) and in the ventral part of the oral pontine reticular nucleus (vRPO). Carbachol in the DOPT produced dissociate polygraphic states, with some but not all REM sleep signs. In contrast, carbachol in the vRPO produced a shift with short latency from wakefulness (W) to REM sleep with all of its polygraphic and behavioral signs. Hcrt-1 in the DOPT increased W and decreased both slow-wave sleep (SWS) and REM sleep during the first 3 h post-drug. The same doses of Hcr-1 in the vRPO produced a significant suppression of REM sleep without a definitive trend for changes in the other states. Both groups showed significant decreases in the number of transitions from SWS to REM sleep. Thus, Hcrt-1 produced distinct effects in cholinoceptive areas of the oral pontine tegmentum; in the DOPT it promoted W, suppressed SWS and probably defacilitated REM sleep, and in the vRPO it directly inhibited REM sleep. Hypocretinergic/orexinergic signaling is lost in narcoleptics and this absence would mean that pontine defacilitation/inhibition of REM sleep would also be absent, explaining why these patients can fall directly into REM sleep from W.
authors.affiliation: Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid, Arzobispo Morcillo 4, 28029 Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Moreno-Balandrán
authors.forename: Miguel
authors.initials: M
authors.lastname: Garzón
authors.forename: Cristina
authors.initials: C
authors.lastname: Bódalo
authors.forename: Fernando
authors.initials: F
authors.lastname: Reinoso-Suárez
authors.forename: Isabel
authors.initials: I
authors.lastname: de Andrés
mesh_terms: D000818:Animals; D002217:Carbachol / Q000008:administration & dosage / Q000494:pharmacology; D002415:Cats; D018679:Cholinergic Agonists / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D047908:Intracellular Signaling Peptides and Proteins / Q000008:administration & dosage* / Q000494:pharmacology; D008845:Microinjections; D009055:Mouth / Q000502:physiology; D009479:Neuropeptides / Q000008:administration & dosage* / Q000494:pharmacology; D000068797:Orexins; D011149:Pons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D011950:Receptors, Cholinergic / Q000378:metabolism; D012890:Sleep / Q000187:drug effects*; D012894:Sleep Stages / Q000187:drug effects; D012895:Sleep, REM / Q000187:drug effects; D013681:Tegmentum Mesencephali / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D014851:Wakefulness / Q000187:drug effects*
chemical_list: D018679:Cholinergic Agonists; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins; D011950:Receptors, Cholinergic; D002217:Carbachol