title: In vitro and in vivo suppression of cellular activity by guanidinoethyl disulfide released from hydrogel microspheres composed of partially oxidized hyaluronan and gelatin.
abstract: This paper describes the preparation of oxidized hyaluronan crosslinked gelatin microspheres for drug delivery. Microspheres were prepared by a modified water-in-oil-emulsion crosslinking method, where three-dimensional crosslinked hydrogel microspheres formed in the absence of any extraneous crosslinker. SEM analyses of the microspheres showed rough surfaces in their dried state with an average diameter of 90 microm. Lyophilization of fully swollen microspheres revealed a highly porous structure. Guanidinoethyl disulfide (GED) was used as a model drug for incorporation into the microspheres; encapsulation of GED was confirmed by HPLC. There was an inverse correlation between the diameters of the microspheres with their GED loading. Macrophage was used as a model cell to evaluate the in vitro efficacy of GED release from the microspheres. The in vivo efficacy of the microspheres was further validated in a mouse full-thickness transcutaneous dermal wound model through suppression of cell infiltration.
authors.affiliation: Department of Biomedical Engineering, T18-030 Health Sciences Center, State University of New York-Stony Brook, Stony Brook, NY 11794-8181, USA.
authors.forename: Lihui
authors.initials: L
authors.lastname: Weng
authors.forename: Natalia D
authors.initials: ND
authors.lastname: Ivanova
authors.forename: Julia
authors.initials: J
authors.lastname: Zakhaleva
authors.forename: Weiliam
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D005780:Gelatin / Q000378:metabolism*; D006146:Guanidines / Q000737:chemistry / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000378:metabolism*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010084:Oxidation-Reduction / Q000187:drug effects; D010316:Particle Size; D012867:Skin / Q000473:pathology
chemical_list: D006146:Guanidines; C014363:bis(2-guanidinoethyl)disulfide; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005780:Gelatin; D006820:Hyaluronic Acid