Only plant-type (GLYK) glycerate kinases produce d-glycerate 3-phosphate.
D-Glycerate kinases (GK) occur in three phylogenetically distinct classes. Class II GKs produce glycerate 2-phosphate, while both class I GK and class III GK (GLYK) are thought to produce glycerate 3-phosphate. We report on the identification of a bacterial-type class I GK in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 and of a plant-type GLYK in the filamentous cyanobacterium Nostoc sp. strain PCC 7120. The comparison with other prokaryotic and eukaryotic GKs of both classes shows that glycerate 3-phosphate is produced only by the GLYKs, but, in contrast to current thinking, not by any of the examined class I enzymes.
Bartsch
Oliver
O
Department of Plant Physiology, University of Rostock, Albert-Einstein-Strasse 3, D-18059 Rostock, Germany.
Hagemann
Martin
M
Bauwe
Hermann
H
D001426:Bacterial Proteins / Q000145:classification / Q000378:metabolism*; D005988:Glyceric Acids / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000145:classification / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D046939:Synechocystis / Q000201:enzymology*
D001426:Bacterial Proteins; D005988:Glyceric Acids; D010940:Plant Proteins; C005156:3-phosphoglycerate; D017853:Phosphotransferases (Alcohol Group Acceptor); C020670:glycerate kinase