Physicochemical and mineralogical characterization of test materials used in 28-day and 90-day intratracheal instillation toxicology studies in rats.
Two recent intratracheal instillation toxicology studies in rats clearly show that a naturally occurring quartz, with occluded crystal surfaces (quartz isolate), produced significantly less inflammatory response than a crushed reference quartz (DQ12). Respirable-size quartz isolate was isolated from bentonite parent rock, without crushing or the use of chemicals, to ensure that the surface properties of the quartz particles were unaltered. The isolation technique utilized gentle mechanical dispersion followed by sedimentation in an aqueous medium. Extensive mineralogical and chemical characterizations were undertaken to define the physicochemical properties of the test materials. The characterizations showed significant, measurable physicochemical differences between the two quartz types. These differences may help to explain the difference in toxicological response associated with these materials. The evaluation methods and resulting data that characterized the chemical and physical properties of the instillation test materials are discussed. The data presented show that such characterizations are essential if meaningful correlations are to be made between test materials and their toxicological profiles.
Miles
W
W
Miles Industrial Mineral Research, Denver, Colorado, USA.
Moll
W F
WF
Hamilton
R D
RD
Brown
R K
RK
D000818:Animals; D001546:Bentonite / Q000737:chemistry*; D007322:Instillation, Drug; D010316:Particle Size; D011791:Quartz / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D051381:Rats; D015203:Reproducibility of Results; D013499:Surface Properties; D013997:Time Factors; D018675:Toxicity Tests / Q000379:methods*; D014132:Trachea
D001546:Bentonite; D011791:Quartz