Chemical constituents of Acanthus ilicifolius L. and effect on osteoblastic MC3T3E1 cells.
A new coumaric acid derivative called acancifoliuside (1) and six known compounds as acteoside (2), isoacteoside (3), acanthaminoside (4), (+)-lyoniresinol 3a-O-beta-glucopyranoside (5), (-)-lyoniresinol (6), and alpha-amyrin (7), were isolated from the methanolic extract of the leaves of Acanthus ilicifolius L. (Acanthaceae). Their structures were determined by spectroscopic methods and a comparison with the spectral data reported in the literature. The effects of the compounds on the function of osteoblastic MC3T3-E1 cells were tested. Compounds 2, 3, and 5 (30 microM) increased the growth and differentiation of osteoblasts significantly (P<0.05), indicating that A. ilicifolius leaves may help prevent osteoporosis.
Van Kiem
Phan
P
Institute of Natural Products Chemistry, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Vietnam.
Quang
Tran Hong
TH
Huong
Tran Thu
TT
Nhung
Le Thi Hong
le TH
Cuong
Nguyen Xuan
NX
Van Minh
Chau
C
Choi
Eun Mi
EM
Kim
Young Ho
YH
D016475:3T3 Cells; D027901:Acanthaceae / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003094:Collagen / Q000378:metabolism; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008968:Molecular Conformation; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013778:Tetrazolium Salts; D013844:Thiazoles
D050071:Bone Density Conservation Agents; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; D003094:Collagen; D000469:Alkaline Phosphatase; C022616:thiazolyl blue; D002118:Calcium