title: Transcriptional analysis of estrogen receptor alpha variant mRNAs in colorectal cancers and their matched normal colorectal tissues.
abstract: Estrogen is involved in suppression of colorectal cancer development and exerts its function via estrogen receptors alpha, beta and their splicing variants. Whether the recently indentified ER-alpha splicing variants, ER-alpha36 and ER-alpha46, play a role in colorectal cancer development is unknown. In this study, we quantified the mRNA copy numbers of wild type ER-alpha (ER-alpha66), ER-alpha46 and ER-alpha36 in 35 colorectal cancers and their matched normal colorectal tissues by quantitative real-time PCR assay, and correlated their mRNA levels with the clinicopathological properties of the tumors. We found that ER-alpha66, ER-alpha46 and ER-alpha36 mRNAs were coexpressed in all colorectal cancers and their matched normal tissues. The decreased mRNA levels of ER-alpha36 and ER-alpha46 whereas no difference of ER-alpha66 mRNA was observed in colorectal cancers compared to their matched normal tissues. Moreover, change in the expression of ER-alpha36 mRNA level was correlated with Dukes' stage of the tumor and the lymph node metastasis. ER-alpha36 mRNA was decreased significantly in Dukes' C+D compared to Dukes' A+B stage tumors (P=0.017), and the expression of ER-alpha36 mRNA in N(1)/N(2) was lower than that in N(0) lymph node metastasis (P=0.049). So ER-alpha36 and ER-alpha46 might be implicated in the development and progression of colorectal cancers.
authors.affiliation: Department of Oncological Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, PR China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Jiang
authors.forename: Rongyue
authors.initials: R
authors.lastname: Teng
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.forename: Haohao
authors.initials: H
authors.lastname: Wang
authors.forename: Zhaoyi
authors.initials: Z
authors.lastname: Wang
authors.forename: Jiang
authors.initials: J
authors.lastname: Cao
authors.forename: Lisong
authors.initials: L
authors.lastname: Teng
mesh_terms: D000328:Adult; D017398:Alternative Splicing; D003106:Colon / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism*; D012007:Rectum / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D012334:RNA, Neoplasm