Presence of neuropeptide Y in somatotrophs of cattle.
Neuropeptide Y (NPY), a 36-amino acid member of the pancreatic polypeptide family, was found to be present by immunohistochemistry in the bovine adenohypophysis. NPY mRNA expression was confirmed in the adenohypophysis by RT-PCR. NPY immunoreactivity was present in about 38% of adenohypophyseal cells in the pars distalis. However, NPY immunoreactive cells (NPY-ir cells) were scarce in the zona tuberalis. Immunohistochemistry of NPY and specific hormones using mirror sections revealed that NPY was colocalized in GH immunoreactive cells. Over 90% of somatotrophs corresponded to NPY-ir cells. These results indicate that endogenous NPY is present in the bovine somatotroph and may act as an endocrine intercellular mediator in the adenohypophysis.
Ogasawara
Hideki
H
Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
Aso
Hisashi
H
Nagai
Yasuhiro
Y
Matsumoto
Kazunori
K
Okamura
Hiroaki
H
Tanaka
Sachi
S
Watanabe
Kouichi
K
Ohwada
Shyuichi
S
Yamaguchi
Takahiro
T
D000818:Animals; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D009478:Neuropeptide Y / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D052683:Somatotrophs / Q000166:cytology / Q000378:metabolism*
D009478:Neuropeptide Y; D012333:RNA, Messenger