Lipoic acid rescues DBA mice from early-onset age-related hearing impairment.
We fed DBA mice with alpha-lipoic acid (100 mg/kg body weight/day), beginning 2, 4, or 8 weeks after birth. Hearing thresholds were measured weekly. At 12 weeks after birth, control mice not fed with alpha-lipoic acid showed significant hearing decreases at all frequencies. In contrast, mice fed with alpha-lipoic acid beginning at 2 weeks after birth showed significantly better hearing at all frequencies. Mice fed with alpha-lipoic acid beginning at 4 and 8 weeks after birth also showed significantly better hearing than control mice after they were fed with alpha-lipoic acid. The stria vascularis of mice fed with alpha-lipoic acid showed reduced 8-oxoguanine residues in DNA and cytoplasm compared with that of control mice. Western blotting showed that the level of hypoxia-inducible factor-1alpha was lower in mice fed with alpha-lipoic acid than in control mice. From these results, we suggest that alpha-lipoic acid prevented early-onset hearing impairment in DBA mice.
Ahn
Joong Ho
JH
Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Head and Neck Surgery, Konkuk University Hospital, Seoul, Korea.
Kang
Hun Hee
HH
Kim
Tae Yong
TY
Shin
Jung-Eun
JE
Chung
Jong Woo
JW
D000367:Age Factors; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000627:therapeutic use; D001309:Auditory Threshold / Q000187:drug effects / Q000502:physiology; D015153:Blotting, Western; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D006309:Hearing / Q000187:drug effects* / Q000502:physiology; D034381:Hearing Loss / Q000503:physiopathology / Q000517:prevention & control*; D006320:Hearing Tests / Q000379:methods; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051379:Mice; D008811:Mice, Inbred DBA; D013316:Stria Vascularis / Q000187:drug effects / Q000378:metabolism; D008063:Thioctic Acid / Q000378:metabolism / Q000627:therapeutic use*
D000975:Antioxidants; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C024829:8-hydroxyguanine; D006147:Guanine; D008063:Thioctic Acid