title: Polymerase chain reaction-based biochemical logic gate coupled with cell-free transcription-translation of green fluorescent protein as a report gate.
abstract: Polymerase chain reaction-based biochemical logic gates were designed for AND, OR, NOT, and AND-NOT operations, whose output signal is reported by coupled cell-free transcription-translation of green fluorescent protein.
authors.affiliation: Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo, Komaba 4-6-1, Meguro-ku, Tokyo 153-8505, Japan. nojima@iis.u-tokyo.ac.jp
authors.forename: Takahiko
authors.initials: T
authors.lastname: Nojima
authors.forename: Takatoki
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
authors.forename: Teruo
authors.initials: T
authors.lastname: Fujii
mesh_terms: D002474:Cell-Free System; D004247:DNA; D049452:Green Fluorescent Proteins / Q000235:genetics*; D044085:Microfluidics; D014176:Protein Biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D049452:Green Fluorescent Proteins; D004247:DNA