[Immunological activity of Streptomyces polysaccharide in normal and immunosuppressed mice].
OBJECTIVE
To investigate the immunological activity of Streptomyces polysaccharide (SMP) on normal and immunosuppressed mice.


METHODS
The effect of SMP on the proliferating activity of normal mouse splenocytes was tested in the mixed lymphocyte culture, and the changes of peripheral blood T lymphocytes were evaluated with acid a-naphthyl acetate esterase (ANAE) method. The ratio of Lyt2+ and L3T4+ T cell subsets was measured by flow cytometry.


RESULTS
SMP stimulated obvious proliferation of mixed lymphocytes, showed protective effects on T lymphocyte and increased the ratio of Lyt2+ and L3T4+ cell subsets to nearly normal level in immunosuppressed mice.


CONCLUSIONS
SMP can regulate the immune function in mice.
Wen
Xiao-yun
XY
22 Hospital of PLA, Geermu 810001, China. wenxy@fimmu.com
Shi
Chao
C
Wu
Shu-guang
SG
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D016867:Immunocompromised Host / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013302:Streptomyces / Q000737:chemistry* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D011134:Polysaccharides