[Development and population structure of mixed (S + M) Pseudomonas aeruginosa cultures in the late stationary growth phase].
Population growth, the ratio between dissociants, pH, and levels of reducing sugars in the medium were monitored during prolonged (375 h) batch cultivation of Pseudomonas aeruginosa S and M dissociants on mineral medium with glucose. During the stationary growth phase (100-375 h), two scenarios were possible. The first one included extensive cell autolysis coupled to alkalinization of the medium and an increased ratio of the M dissociant. In the second case, acidification of the medium was coupled to the oscillating secondary growth, mostly of the M dissociant; the dynamics of cell numbers of this dissociant correlated with the dynamics of the culture optical density. In this scenario, periodical appearance of reducing sugars in the medium was detected; it was in the opposite phase with the changes of the M dissociant cell numbers. The differences between scenarios of P. aeruginosa growth in the late stationary phase were probably due to the physiological and biochemical characteristics of the S and M dissociants, including different pathways of glucose utilization (respiration or fermentation), resistance to acidification, synthetic (proteolytic) activity, and productivity of autoinducers.
Mil'ko
E S
ES
Kreĭer
V G
VG
Egorov
N S
NS
Loĭko
N G
NG
Golod
N A
NA
D001433:Bacteriolysis; D018929:Cell Culture Techniques; D003470:Culture Media; D005285:Fermentation; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D005947:Glucose