title: Legume lectin FRIL preserves neural progenitor cells in suspension culture in vitro.
abstract: In vitro maintenance of stem cells is crucial for many clinical applications. Stem cell preservation factor FRIL (Flt3 receptor-interacting lectin) is a plant lectin extracted from Dolichos Lablab and has been found preserve hematopoietic stem cells in vitro for a month in our previous studies. To investigate whether FRIL can preserve neural progenitor cells (NPCs), it was supplemented into serum-free suspension culture media. FRIL made NPC grow slowly, induced cell adhesion, and delayed neurospheres formation. However, FRIL did not initiate NPC differentiation according to immunofluorescence and semiquantitive RT-PCR results. In conclusion, FRIL could also preserve neural progenitor cells in vitro by inhibiting both cell proliferation and differentiation.
authors.affiliation: Stem Cells and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Hailei
authors.initials: H
authors.lastname: Yao
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xie
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.forename: Shu
authors.initials: S
authors.lastname: Han
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Shi
authors.forename: Xue
authors.initials: X
authors.lastname: Nan
authors.forename: Cixian
authors.initials: C
authors.lastname: Bai
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xuetao
authors.initials: X
authors.lastname: Pei
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D007887:Fabaceae / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry / Q000235:genetics; D037241:Mannose-Binding Lectins / Q000494:pharmacology*; D037121:Plant Lectins / Q000494:pharmacology*; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D014021:Tissue Preservation
chemical_list: D016895:Culture Media, Serum-Free; C117675:FRIL protein, Dolichos lablab; D037102:Lectins; D037241:Mannose-Binding Lectins; D037121:Plant Lectins