Intestinal smooth muscle cell maintenance by basic fibroblast growth factor.
Intestinal tissue engineering is a potential therapy for patients with short bowel syndrome. Tissue engineering scaffolds that promote smooth muscle cell proliferation and angiogenesis are essential toward the regeneration of functional smooth muscles for peristalsis and motility. Since basic fibroblast growth factor (bFGF) can stimulate smooth muscle proliferation and angiogenesis, the delivery of bFGF was employed to stimulate proliferation and survival of primary intestinal smooth muscle cells. Two methods of local bFGF delivery were examined: the incorporation of bFGF into the collagen coating and the encapsulation of bFGF into poly(D,L-lactic-co-glycolic acid) microspheres. Cell-seeded scaffolds were implanted into the omentum and were retrieved after 4, 14, and 28 days. The seeded cells proliferated from day 4 to day 14 in all implants; however, at 28 days, significantly higher density of implanted cells and blood vessels was observed, when 10 microg of bFGF was incorporated into the collagen coating of scaffolds as compared to scaffolds with either no bFGF or 1 microg of bFGF in collagen. Microsphere encapsulation of 1 microg of bFGF produced similar effects as 10 microg of bFGF mixed in collagen and was more effective than the delivery of 1 microg of bFGF by collagen incorporation. The majority of the implanted cells also expressed alpha-smooth muscle actin. Scaffolds coated with microsphere-encapsulated bFGF and seeded with smooth muscle cells may be a useful platform for the regeneration of the intestinal smooth muscle.
Lee
Min
M
Department of Bioengineering, University of California, Los Angeles, California 90095, USA.
Wu
Benjamin M
BM
Stelzner
Matthias
M
Reichardt
Holger M
HM
Dunn
James C Y
JC
D000199:Actins / Q000378:metabolism; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D003094:Collagen / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000166:cytology*; D008863:Microspheres; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects*; D051381:Rats; D011917:Rats, Inbred Lew; D054457:Tissue Scaffolds; D014841:von Willebrand Factor / Q000378:metabolism
D000199:Actins; C485184:enhanced green fluorescent protein; D014841:von Willebrand Factor; D016222:Fibroblast Growth Factor 2; D049452:Green Fluorescent Proteins; D003094:Collagen