title: Chaotropic salts: novel modifiers for the capillary electrophoretic analysis of benzodiazepines.
abstract: This paper describes a CE method for analyzing benzodiazepines using the chaotropic salts lithium trifluoromethanesulfonate (LiOTf), lithium hexafluorophosphate (LiPF(6)), and lithium bis(trifluoromethanesulfonyl)imide (LiNTf(2)) as modifiers in the running buffer. Although adequate resolution of seven benzodiazepine analytes occurred under the influence of each of the chaotropic anions, the separation efficiency was highest when bis(trifluoromethanesulfonyl)imide (Tf(2)N(-)) was the modifier. We applied affinity CE in conjunction with linear analysis to determine the association constants for the formation of complexes between the Tf(2)N(-) anion and the benzodiazepines. According to the estimated Gibbs free energies, the interactions between this chaotropic anion and the benzodiazepines were either ion-dipole or ion-induced dipole interactions. Adding chaotropic salts as modifiers into CE buffers is a simple and reproducible technique for separating benzodiazepines.
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, ROC.
authors.forename: Hsiu-Li
authors.initials: HL
authors.lastname: Su
authors.forename: Min-Tsu
authors.initials: MT
authors.lastname: Lan
authors.forename: Kuan-Wen
authors.initials: KW
authors.lastname: Lin
authors.forename: You-Zung
authors.initials: YZ
authors.lastname: Hsieh
mesh_terms: D000838:Anions / Q000737:chemistry*; D001569:Benzodiazepines / Q000032:analysis* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods; D018020:Lithium Compounds / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*
chemical_list: D000838:Anions; D018020:Lithium Compounds; D012492:Salts; D001569:Benzodiazepines