title: Global analysis of the meiotic crossover landscape.
abstract: Tight control of the number and distribution of crossovers is of great importance for meiosis. Crossovers establish chiasmata, which are physical connections between homologous chromosomes that provide the tension necessary to align chromosomes on the meiotic spindle. Understanding the mechanisms underlying crossover control has been hampered by the difficulty in determining crossover distributions. Here, we present a microarray-based method to analyze multiple aspects of crossover control simultaneously and rapidly, at high resolution, genome-wide, and on a cell-by-cell basis. Using this approach, we show that loss of interference in zip2 and zip4/spo22 mutants is accompanied by a reduction in crossover homeostasis, thus connecting these two levels of crossover control. We also provide evidence to suggest that repression of crossing over at telomeres and centromeres arises from different mechanisms. Lastly, we uncover a surprising role for the synaptonemal complex component Zip1 in repressing crossing over at the centromere.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
authors.forename: Stacy Y
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Tsubouchi
authors.affiliation: Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
authors.forename: Beth
authors.initials: B
authors.lastname: Rockmill
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
authors.forename: Jay S
authors.initials: JS
authors.lastname: Sandler
authors.affiliation: Ingenuity Systems, Inc., Redwood City, CA 94063, USA.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Richards
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
authors.forename: Gerben
authors.initials: G
authors.lastname: Vader
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hochwagen
authors.affiliation: Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA; Department of Genetics, Yale University, New Haven, CT 06520, USA.
authors.forename: G Shirleen
authors.initials: GS
authors.lastname: Roeder
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: jfung@biochem.ucsf.edu.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Fung
mesh_terms: D001483:Base Sequence; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D002842:Chromatids / Q000378:metabolism; D015825:Chromosomes, Fungal; D003434:Crossing Over, Genetic / Q000235:genetics*; D005819:Genetic Markers; D006706:Homeostasis; D008540:Meiosis / Q000502:physiology*; D046228:Microarray Analysis / Q000379:methods*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013573:Synaptonemal Complex / Q000235:genetics / Q000378:metabolism; D016615:Telomere / Q000235:genetics / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; C090118:Zip1 protein, S cerevisiae