Dividable membrane with multi-reaction wells for microarray biochips.
This paper presents a multi-well membrane fabricated using polydimethylsiloxane (PDMS) as a part of a microarray biochip that allows dividable incubation chambers to be provided on a single chip. The conditions of the forming temperature, time, and mixing proportion of the materials were investigated to obtain optimal physical absorption with the surface of the chip substrate. To verify the properties of the multi-well chip, immunoassays were performed by the alpha-1-fetoprotein (AFP) antigen sandwich experiment. The results showed that the detection limit reached to the concentration of 10 ng/ml AFP antigen, and that the dynamic range was 30-3000 ng/ml. Attaining excellent physical absorption helps in avoiding cross-contamination or interference between different samples on the same biochip. The merits of dividable multi-well chips include promoting effective use of surface and multiple-sample experiments.
Chang
Yaw-Jen
YJ
Department of Mechanical Engineering, Chung Yuan Christian University, 200 Chung Pei Rd., Chung Li, Taiwan 32023, ROC. justin@cycu.edu.tw
Hu
Chih-Yu
CY
Yin
Li-Te
LT
Chang
Cheng-Hao
CH
Su
Hung-Ju
HJ
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008567:Membranes, Artificial; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D009757:Nylons / Q000737:chemistry*
D004129:Dimethylpolysiloxanes; D008567:Membranes, Artificial; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer