Antifibrotic activity of diterpenes from Biota orientalis leaves on hepatic stellate cells.
Antifibrotic effect of twelve diterpenes (1-12) from the 90% methanolic fraction of Biota orientalis leaves was evaluated employing HSC-T6 cells by assessing cell proliferation and morphological change. Among these diterpenes, totarol (8) and isopimara-8(14),15-dien-19-oic acid (9) dramatically reduced cell proliferation in dose-and time-dependent manner. Furthermore, treatment with these compounds resulted in the different pattern of morphological changes of HSC-T6 cells. Taken together, antiproliferative activity of diterpenes from B. orientalis might suggest therapeutic potentials against liver fibrosis.
Lee
Mi Kyeong
MK
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
Yang
Hyekyung
H
Yoon
Jeong Seon
JS
Jeong
Eun Ju
EJ
Kim
Do Yoon
DY
Ha
Na Ry
NR
Sung
Sang Hyun
SH
Kim
Young Choong
YC
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D029779:Cupressaceae / Q000737:chemistry*; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008103:Liver Cirrhosis / Q000473:pathology / Q000517:prevention & control*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats
D004224:Diterpenes; D007770:L-Lactate Dehydrogenase