title: Application of Mie theory to assess structure of spheroidal scattering in backscattering geometries.
abstract: Inverse light scattering analysis seeks to associate measured scattering properties with the most probable theoretical scattering distribution. Although Mie theory is a spherical scattering model, it has been used successfully for discerning the geometry of spheroidal scatterers. The goal of this study was an in-depth evaluation of the consequences of analyzing the structure of spheroidal geometries, which are relevant to cell and tissue studies in biology, by employing Mie-theory-based inverse light scattering analysis. As a basis for this study, the scattering from spheroidal geometries was modeled using T-matrix theory and used as test data. In a previous study, we used this technique to investigate the case of spheroidal scatterers aligned with the optical axis. In the present study, we look at a broader scope which includes the effects of aspect ratio, orientation, refractive index, and incident light polarization. Over this wide range of parameters, our results indicate that this method provides a good estimate of spheroidal structure.
authors.affiliation: Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, NC 27708, USA. kevin.chalut@duke.edu
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Chalut
authors.forename: Michael G
authors.initials: MG
authors.lastname: Giacomelli
authors.forename: Adam
authors.initials: A
authors.lastname: Wax
mesh_terms: D000818:Animals; D004848:Epithelium / Q000473:pathology; D006801:Humans; D008027:Light; D015233:Models, Statistical; D008962:Models, Theoretical; D055095:Optics and Photonics; D015203:Reproducibility of Results; D012542:Scattering, Radiation