Bilayer disk capillary electrophoresis: a novel method to study drug partitioning into membranes.
CE in the presence of lipid bilayer disks was introduced as a new approach in membrane partitioning studies. The disks were used as a pseudostationary phase in the partial-filling mode of CE and the partitioning of cationic drugs was determined. The migration times of the analytes increased linearly with the lipid amount in the system. An appropriate algorithm for the calculation of a partition coefficient is presented. In the disk-shaped bilayers, which have excellent stability and shelf life, all of the lipids are readily available for interaction and the disks can be used as realistic cell membrane models.
Boija
Elisabet
E
Department of Biochemistry and Organic Chemistry, Biomedical Center, Uppsala University, Uppsala, Sweden.
Lundquist
Anna
A
Nilsson
Mikael
M
Edwards
Katarina
K
Isaksson
Roland
R
Johansson
Gunnar
G
D019075:Electrophoresis, Capillary / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013499:Surface Properties
D008051:Lipid Bilayers; D004364:Pharmaceutical Preparations