Biochemical characterization of soluble proteins in pecan [Carya illinoinensis (Wangenh.) K. Koch].
Pecans (cv. Desirable) contained approximately 10% protein on a dry weight basis. The minimum nitrogen solubility (5.9-7.5%) at 0.25-0.75 M trichloroacetic acid represented the nonprotein nitrogen. Among the solvents assessed for protein solubilization, 0.1 M NaOH was the most effective, while borate saline buffer (pH 8.45) was judged to be optimal for protein solubilization. The protein solubility was minimal in the pH range of 3-7 and significantly increased on either side of this pH range. Increasing the NaCl concentration from 0 to 4 M significantly improved ( approximately 8-fold increase) protein solubilization. Following Osborne protein fractionation, the alkali-soluble glutelin fraction (60.1%) accounted for a major portion of pecan proteins followed by globulin (31.5%), prolamin (3.4%), and albumin (1.5%), respectively. The majority of pecan polypeptides were in the molecular mass range of 12-66 kDa and in the pI range of 4.0-8.3. The pecan globulin fraction was characterized by the presence of several glycoprotein polypeptides. Lysine was the first limiting essential amino acid in the defatted flour, globulin, prolamin, and alkaline glutelin fractions. Leucine and tryptophan were the first limiting essential amino acids in albumin and acid glutelin fractions, respectively. Rabbit polyclonal antibodies detected a range of pecan polypeptides in the 12-60 kDa range, of which the globulin fraction contained the most reactive polypeptides.
Venkatachalam
Mahesh
M
Department of Nutrition, Food and Exercise Sciences, Florida State University, 402 Sandels Building, College of Human Sciences, Tallahassee, Florida 32306-1493, USA.
Roux
Kenneth H
KH
Sathe
Shridhar K
SK
D000596:Amino Acids / Q000032:analysis; D031325:Carya / Q000737:chemistry*; D005591:Chemical Fractionation; D005983:Glutens / Q000032:analysis; D015151:Immunoblotting; D021184:Nut Hypersensitivity / Q000276:immunology; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D012639:Seeds / Q000737:chemistry*; D012995:Solubility
D000596:Amino Acids; D010455:Peptides; D010940:Plant Proteins; D005983:Glutens