title: Inverse relationship between the length of the EGFR CA repeat polymorphism in lung carcinoma and protein expression of EGFR in the carcinoma.
abstract: OBJECTIVE
  There is a CA dinucleotide repeat polymorphism in the first intron of the epidermal growth factor receptor (EGFR) gene. Our aim was to examine the relationship between the CA repeat length in lung carcinoma and EGFR mutation, EGFR gene copy number, and EGFR protein expression level in the carcinoma.
  
  
  METHODS
  We examined 168 lung carcinomas for the length of the CA repeat polymorphism by PCR-polyacrylamide gel electrophoresis analysis, for EGFR mutations by sequencing analysis, for EGFR gene copy number by fluorescence in situ hybridization analysis, and for EGFR protein expression by immunohistochemical analysis.
  
  
  RESULTS
  When the carcinomas were divided into two groups according to the length of their CA repeat polymorphism, the EGFR protein expression level was found to be significantly higher in the shorter allele group than in the longer allele group (P = 0.0116), but its length was not associated with EGFR somatic mutations, high EGFR gene copy numbers, or clinicopathological factors, such as histological type or stage.
  
  
  CONCLUSIONS
  The results suggested that the length of the EGFR CA repeat polymorphism in lung carcinoma is inversely related with level of EGFR protein expression in the carcinoma.
authors.affiliation: 1st Department of Pathology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Suzuki
authors.forename: Shinji
authors.initials: S
authors.lastname: Kageyama
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shinmura
authors.forename: Koji
authors.initials: K
authors.lastname: Okudela
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Bunai
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Nagura
authors.forename: Hisaki
authors.initials: H
authors.lastname: Igarashi
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Kiyose
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mori
authors.forename: Hong
authors.initials: H
authors.lastname: Tao
authors.forename: Masanori
authors.initials: M
authors.lastname: Goto
authors.forename: Kazuya
authors.initials: K
authors.lastname: Takamochi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Kazuya
authors.initials: K
authors.lastname: Suzuki
authors.forename: Riuko
authors.initials: R
authors.lastname: Ohashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ogawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Niwa
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Tsuneyoshi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sugimura
mesh_terms: D000368:Aged; D002277:Carcinoma / Q000235:genetics*; D004252:DNA Mutational Analysis; D018900:Dinucleotide Repeats; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007438:Introns / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011110:Polymorphism, Genetic
chemical_list: D066246:ErbB Receptors