title: Uptake mechanism of oppositely charged fluorescent nanoparticles in HeLa cells.
abstract: The endocytotic mechanisms involved in the uptake of charged polystyrene nanoparticles into HeLa cells were investigated. Uptake experiments were done in the presence or absence of drugs known to inhibit various factors in endocytosis. Independent of the particle charge, endocytosis is highly dependent on dynamin, F-actin, and tyrosine-specific protein kinases, which suggests a dynamin-dependent and lipid raft-dependent mechanism. However, cholesterol depletion did not hinder particle uptake. Regarding positively charged particles, macropinocytosis, the microtubule network, and cyclooxygenases are also involved. The clathrin-dependent pathway plays a minor role.
authors.affiliation: Institute of Organic Chemistry III, Macromolecular Chemistry and Organic Materials, University of Ulm, Ulm, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Dausend
authors.forename: Anna
authors.initials: A
authors.lastname: Musyanovych
authors.forename: Martin
authors.initials: M
authors.lastname: Dass
authors.forename: Paul
authors.initials: P
authors.lastname: Walther
authors.forename: Hubert
authors.initials: H
authors.lastname: Schrezenmeier
authors.forename: Katharina
authors.initials: K
authors.lastname: Landfester
authors.forename: Volker
authors.initials: V
authors.lastname: Mailänder
mesh_terms: D002784:Cholesterol / Q000378:metabolism; D004705:Endocytosis; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles
chemical_list: D002784:Cholesterol