title: Synthesis of glyceryl ferulate by immobilized ferulic acid esterase.
abstract: Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL "Amano" from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50 degrees C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25 degrees C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Takemasa
authors.initials: T
authors.lastname: Matsuo
authors.forename: Takashi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Kimura
authors.forename: Moriyasu
authors.initials: M
authors.lastname: Tsuchiyama
authors.forename: Tadanobu
authors.initials: T
authors.lastname: Oh
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Shuji
authors.initials: S
authors.lastname: Adachi
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D050178:Monoglycerides / Q000096:biosynthesis*; D011096:Polygalacturonase / Q000378:metabolism; D012995:Solubility; D013696:Temperature
chemical_list: D003373:Coumaric Acids; D004800:Enzymes, Immobilized; D050178:Monoglycerides; C531553:glyceryl ferulate; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D011096:Polygalacturonase; D005990:Glycerol