Effects of alcohol-induced human peripheral blood mononuclear cell (PBMC) pretreated whey protein concentrate (WPC) on oxidative damage.
Excessive alcohol consumption can induce apoptosis in a variety of tissues and influence the antioxidant status in peripheral blood mononuclear cells (PBMC). This paper investigates the effects of whey protein concentrate (WPC) pretreated in PBMC on the apoptosis and antioxidant status after the treatment of alcohol. The results show that the percentages of apoptotic cells in the alcohol-treated group were higher than those in the group without alcohol treatment. Additionally, there was higher glutathione (GSH) peroxidase (GPx) activity when the PBMC were treated with 300 mg/dL of alcohol. With regard to the activity of GSH reductase (GRx), there was higher activity in the group pretreated with WPC than in the group with the treatment of alcohol only. On the contrary, the levels of GSH were reduced after the treatment of alcohol, but there was a higher level of GSH in the group pretreated with WPC. In this study, it was found that the increased level of GSH in PBMC might not be attributed to the effect of GRx because there was still a higher level of GSH in the group with the treatment of WPC and BCNU (a GRx inhibitor) in this study. The results indicated that PBMC pretreated with WPC might ameliorate alcohol-induced effects such as imbalance of the antioxidant status.
Tseng
Yang-Ming
YM
Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Chen
Sheng-Yi
SY
Chen
Chien-Hung
CH
Jin
Yi-Ru
YR
Tsai
Shih-Meng
SM
Chen
Ing-Jun
IJ
Lee
Jang-Hwa
JH
Chiu
Chzng-Cheng
CC
Tsai
Li-Yu
LY
D017209:Apoptosis / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008894:Milk Proteins / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D000067816:Whey Proteins
D008894:Milk Proteins; D000067816:Whey Proteins; D000431:Ethanol; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione