title: ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome.
abstract: The small serine resolvase ParA from bacterial plasmids RK2 and RP4 catalyzes the recombination of two identical 133 bp recombination sites known as MRS. Previously, we reported that ParA is active in the fission yeast Schizosaccharomyces pombe. In this work, the parA recombinase gene was placed under the control of the Arabidopsis OXS3 promoter and introduced into Arabidopsis lines harboring a chromosomally integrated MRS-flanked target. The ParA recombinase excised the MRS-flanked DNA and the excision event was detected in subsequent generations in the absence of ParA, indicating germinal transmission of the excision event. The precise site-specific deletion by the ParA recombination system in planta demonstrates that the ParA recombinase can be used to remove transgenic DNA, such as selectable markers or other introduced transgenes that are no longer desired in the final product.
authors.affiliation: Plant Gene Expression Center, 800 Buchanan Street, Albany, CA 94710, USA. jthomson@pw.usda.gov
authors.forename: James G
authors.initials: JG
authors.lastname: Thomson
authors.forename: Yuan-Yeu
authors.initials: YY
authors.lastname: Yau
authors.forename: Robert
authors.initials: R
authors.lastname: Blanvillain
authors.forename: Wylla M
authors.initials: WM
authors.lastname: Nunes
authors.forename: Dawn
authors.initials: D
authors.lastname: Chiniquy
authors.forename: Roger
authors.initials: R
authors.lastname: Thilmony
authors.forename: David W
authors.initials: DW
authors.lastname: Ow
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D032461:Chromosomes, Plant / Q000235:genetics; D004247:DNA / Q000235:genetics*; D005821:Genetic Techniques; D018745:Genome, Plant; D008957:Models, Genetic; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D045522:Recombinases / Q000235:genetics*; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D045522:Recombinases; C052883:chromosome partition proteins, bacterial; D004247:DNA