Biological properties of crosslinked salmon collagen fibrillar gel as a scaffold for human umbilical vein endothelial cells.
Collagen derived from chum salmon (Oncorhynchus keta) was crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) during collagen fibrillogenesis and applied to an in vitro cell culture to evaluate its potential use as a scaffold for vascular tissue engineering. Human umbilical vein endothelial cells (HUVEC) were cultured on the crosslinked salmon collagen fibrillar gel (EDC-SC gel), and their growth rates and production levels of cytokines, including platelet-derived growth factor-BB and von Willebrand factor, were measured. Comparison was also made with bovine collagen gel crosslinked with EDC (EDC-BC gel). The growth and cytokine production of the HUVEC cultured on the EDC-SC gel were higher than those on the EDC-BC gel. In addition, HUVEC were found to attach to the EDC-BC gel through alpha2beta1 integrin for native collagen, whereas they attached to the EDC-SC gel through alphavbeta3 integrin for denatured collagen as well as the alpha2beta1 integrin, indicating that HUVEC recognized denatured domains in the EDC-SC gel. In conclusion, the EDC-SC gel can be used as a scaffold to support HUVEC growth, although the integrin-mediated attachment manner differs between the two gels.
Nagai
Nobuhiro
N
Creative Research Initiative 'Sousei' (CRIS), Hokkaido University N21-W10, Kita-ku, Sapporo, Hokkaido 001-0021, Japan. nagai491@yahoo.co.jp
Mori
Kazuo
K
Munekata
Masanobu
M
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D024022:Fibrillar Collagens / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D012474:Salmon / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D024022:Fibrillar Collagens; D005782:Gels