RANKL, osteopontin, and osteoclast homeostasis in a hyperocclusion mouse model.
The biological mechanisms that maintain the position of teeth in their sockets establish a dynamic equilibrium between bone resorption and apposition. In order to reveal some of the dynamics involved in the tissue responses towards occlusal forces on periodontal ligament (PDL) and alveolar bone homeostasis, we developed the first mouse model of hyperocclusion. Swiss-Webster mice were kept in hyperocclusion for 0, 3, 6, and 9 d. Morphological and histological changes in the periodontium were assessed using micro-computed tomography (micro-CT) and ground sections with fluorescent detection of vital dye labels. Sections were stained for tartrate-resistant acid phosphatase, and the expression of receptor activator of nuclear factor-kappaB ligand (RANKL) and osteopontin (OPN) was analyzed by immunohistochemistry and real-time polymerase chain reaction (PCR). Traumatic occlusion resulted in enamel surface abrasion, inhibition of alveolar bone apposition, significant formation of osteoclasts at 3, 6 and 9 d, and upregulation of OPN and RANKL. Data from this study suggest that both OPN and RANKL contribute to the stimulation of bone resorption in the hyperocclusive state. In addition, we propose that the inhibition of alveolar bone apposition by occlusal forces is an important mechanism for the control of occlusal height that might work in synergy with RANKL-induced bone resorption to maintain normal occlusion.
Walker
Cameron G
CG
Department of Oral Biology, University of Illinois at Chicago, College of Dentistry, Chicago, IL 60612, USA.
Ito
Yoshihiro
Y
Dangaria
Smit
S
Luan
Xianghong
X
Diekwisch
Thomas G H
TG
D016301:Alveolar Bone Loss / Q000209:etiology; D000818:Animals; D016723:Bone Remodeling; D049109:Cell Proliferation; D003769:Dental Occlusion, Traumatic / Q000150:complications / Q000503:physiopathology*; D005347:Fibroblasts / Q000473:pathology; D006706:Homeostasis; D007150:Immunohistochemistry; D051379:Mice; D023421:Models, Animal; D010010:Osteoclasts / Q000378:metabolism* / Q000502:physiology; D053495:Osteopontin / Q000096:biosynthesis*; D010513:Periodontal Ligament / Q000473:pathology / Q000503:physiopathology*; D016133:Polymerase Chain Reaction; D053245:RANK Ligand / Q000096:biosynthesis*; D019217:Tooth Attrition / Q000209:etiology
D053245:RANK Ligand; D053495:Osteopontin