Determination of branched beta-cyclodextrin-prostaglandin complexes using electrospray ionization mass spectrometry.
Mass spectral measurements by electrospray ionization mass spectrometry (ESI-MS) detected the ions of beta-cyclodextrin (betaCD) or branched betaCDs (glucosyl-, galactosyl-, mannosyl- and maltosyl-betaCD)-prostaglandins (PGs: PGA(2), PGD(2), PGE(1), PGE(2), PGF(2alpha) and PGJ(2)) complexes, i.e., betaCD-PG complexes, with a host:guest ratio of 1:1 in the negative ion mode. This is the first study to report the ions of branched betaCD-PG complexes using ESI-MS. The inclusion complexes were determined by a flow injection analysis using acetonitrile/water. We could confirm by this method the presence of a betaCD-PGE(2) complex with a host:guest ratio of 1:1 in a solution-dissolved pharmaceutical formulation consisting of betaCD-PGE(2) (Prostarmon E tablet).
Yamane
Naoe
N
JCL Bioassay Corporation, Nishiwaki, Japan. n.yamane@jclbio.com
Tozuka
Zenzaburo
Z
Okada
Yasuyo
Y
Honda
Chie
C
Nishi
Yuki
Y
Tanimoto
Toshiko
T
D008958:Models, Molecular; D015394:Molecular Structure; D011453:Prostaglandins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D047392:beta-Cyclodextrins / Q000032:analysis* / Q000737:chemistry*
D011453:Prostaglandins; D047392:beta-Cyclodextrins; C031215:betadex