Analysis and purification of alcohol-sensitive chiral compounds using 2,2,2-trifluoroethanol as a modifier in supercritical fluid chromatography.
2,2,2-Trifluoroethanol (TFE) is evaluated as an alternative modifier for the analysis and purification of alcohol-sensitive chiral compounds using supercritical fluid chromatography (SFC). Four chiral compounds, selected for their sensitivity to alcohols, in addition to a variety of standard chiral compounds were analyzed by SFC using TFE with polysaccharide and Pirkle-type chiral stationary phases (CSPs) to produce selectivities (alpha) and resolutions (Rs) as high as 1.4 and 7.2. A preparative isolation of 2-phenylglutaric anhydride was achieved using TFE as the mobile phase modifier to produce clean enantiomers.
Byrne
Neal
N
Lotus Separations, LLC, Princeton University, Washington Road, Princeton, NJ 08544, USA.
Hayes-Larson
Eleanor
E
Liao
Wei-Wei
WW
Kraml
Christina M
CM
D000812:Anhydrides / Q000302:isolation & purification*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D005977:Glutarates / Q000302:isolation & purification*; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D013237:Stereoisomerism; D014270:Trifluoroethanol / Q000737:chemistry*
C538977:2-phenylglutaric anhydride; D000812:Anhydrides; D005977:Glutarates; D004364:Pharmaceutical Preparations; D014270:Trifluoroethanol