Cloning and characterization of myosin regulatory light chain (MRLC) gene from Culex pipiens pallens.
Myosin regulatory light chain (MRLC) (GenBank accession no. DQ140391) was cloned from Culex pipiens pallens. An open reading frame (ORF) of 630 bps was found to encode a putative 210 amino acids protein which shows 73% similarity with myosin regulatory light chain of Gryllotalpa orientalis. Real-time quantitative PCR analysis demonstrated that the transcription level of MRLC in deltamethrin-resistant strain (DR-strain) was 4.08-fold higher than in deltamethrin-susceptible strain (DS-strain) of C. pipiens pallens. Over-expression of MRLC in Aedes albopictus C6/36 cells conferred protection against deltamethrin based on tritiated methyl tritiated thymidine ((3)H-TdR) incorporation assay. These results indicate that MRLC may be a potential cause of deltamethrin resistance in C. pipiens pallens.
Yang
Mifang
M
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 210029, PR China.
Qian
Jin
J
Sun
Jing
J
Xu
Yang
Y
Zhang
Donghui
D
Ma
Lei
L
Sun
Yan
Y
Zhu
Changliang
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology; D008969:Molecular Sequence Data; D018994:Myosin Light Chains / Q000235:genetics*; D009570:Nitriles / Q000494:pharmacology; D010802:Phylogeny; D011722:Pyrethrins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D018076:DNA, Complementary; D019476:Insect Proteins; D007306:Insecticides; D018994:Myosin Light Chains; D009570:Nitriles; D011722:Pyrethrins; D011994:Recombinant Proteins; C017180:decamethrin