title: Role of MAPK in mechanical force-induced up-regulation of type I collagen and osteopontin in human gingival fibroblasts.
abstract: In addition to periodontal ligament, the gingival plays an important role in alveolar bone remodeling induced by physiological and mechanical stimuli. However, there are few reports showing the cellular responses of human gingival fibroblasts (HGF) to a mechanical force. This study examined the effects of centrifugal force on the proliferation of the bone tissue components, such as type I collagen (COL I), osteopontin (OPN), and osteonectin (ONN) in the HGF. The roles of extracellular signal-regulated kinase (ERK), c-Jun-N-terminal kinase (JNK), and p-38 kinase were also investigated. Centrifugal force induced cell cycle arrest in the G(1) phase without any cytotoxic effects and increased the levels of COL I and OPN expression in the cells but had no effect on ONN. The force-induced up-regulation of COL I was found to be mediated by both the ERK-c-Fos-COL I and JNK-c-Jun-COL I pathways, while that of OPN was mediated only by the ERK-mediated pathway. Our present findings suggest that centrifugal force up-regulates COL I and OPN expression in HGF, where both ERK and JNK play indispensable roles.
authors.affiliation: School of Dentistry and Institute of Oral Biosciences, Chonbuk National University, Jeonju 561-756, South Korea.
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Jeon
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Kook
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Son
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Kim
authors.forename: Soon-Sun
authors.initials: SS
authors.lastname: Park
authors.forename: Jong-Ghee
authors.initials: JG
authors.lastname: Kim
authors.forename: Jeong-Chae
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000328:Adult; D002453:Cell Cycle / Q000502:physiology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D013314:Stress, Mechanical; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D024042:Collagen Type I; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1; D053495:Osteopontin; D020928:Mitogen-Activated Protein Kinases