title: Protective effects of a heme oxygenase-1-secreting Lactococcus lactis on mucosal injury induced by hemorrhagic shock in rats.
abstract: OBJECTIVE
  To investigate the protective effects of a heme oxygenase-1 (HO-1)-secreting Lactococcus lactis (LL-HO-1) on mucosal injury induced by hemorrhagic shock in rats.
  
  
  METHODS
  The ability of recombinant LL-HO-1 to secrete biological active HO-1 in the rat intestine was determined in situ after 3 d of daily intragastric administration. The therapeutic potential of LL-HO-1 strain was then evaluated on mucosal injury induced by hemorrhagic shock in rats. After successful resuscitation, mean arterial blood pressure was recorded at 5, 10, 20, and 30 min. One hour after resuscitation, the ileum was harvested for evaluation of mucosal injury by blinded microscopic inflammatory score (Chiu's grade 0-5), myeloperoxidase activity, bacterial translocation, and by the secretion of pro- and anti-inflammatory cytokines (tumor necrosis factor-alpha and interleukin-10, respectively).
  
  
  RESULTS
  Intragastric administration of HO-1-secreting L. lactis strain led to bioactive delivery of HO-1 at intestinal mucosa and significantly enhanced mean arterial blood pressure and interleukin-10 levels. Moreover, intragastric administration of LL-HO-1 significantly decreased Chiu's score, myeloperoxidase activity, bacterial translocation, and tumor necrosis factor-alpha levels when compared with rats treated with the wild-type strain. The protective effect of recombinant LL-HO-1 could be abolished by co-administration of a HO-1 inhibitor, the zinc protoporphyrin-IX.
  
  
  CONCLUSIONS
  These results suggest that intragastric administration with HO-1-secreting L. lactis reduces mucosal injury induced by hemorrhagic shock.
authors.affiliation: Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu Province, People's Republic of China.
authors.forename: Qing-feng
authors.initials: QF
authors.lastname: Pang
authors.forename: Yong
authors.initials: Y
authors.lastname: Ji
authors.forename: Luis G
authors.initials: LG
authors.lastname: Bermúdez-Humarán
authors.forename: Qiao-mei
authors.initials: QM
authors.lastname: Zhou
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.forename: Yinming
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D007082:Ileum; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology; D013294:Lactococcus lactis / Q000378:metabolism*; D019936:Probiotics / Q000378:metabolism*; D051381:Rats; D012771:Shock, Hemorrhagic / Q000150:complications*
chemical_list: D051547:Heme Oxygenase-1