Preparative isolation and purification of two new isomeric diterpenoid alkaloids from Aconitum coreanum by high-speed counter-current chromatography.
Preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was used to isolate and separate bioactive constituents from the roots of Aconitum coreanum. Two new diterpenoid alkaloid isomers were successfully separated for the first time by HSCCC with an optimized two-phase solvent system composed of ethyl acetate-n-butanol-methanol-2% acetic acid (3.5:1.5:2:4.5, v/v/v/v), 25.4 mg of GFT (1) and 18.3 mg of GFU (2) were isolated form 1 g crude extract in one step HSCCC experiment. The purities of the two new compounds were all over 95% as analyzed by HPLC and their structures were identified by ESI-MS, (1)H NMR, (13)C NMR, and 2D NMR analysis.
Tang
Qingfa
Q
Key Laboratory of Research of Traditional Chinese Medicine and New Drug, Southern Medical University, Guangzhou 510515, China.
Liu
Jinghan
J
Xue
Jiao
J
Ye
Wencai
W
Zhang
Zunjian
Z
Yang
Chunhua
C
D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D004224:Diterpenes / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D021241:Spectrometry, Mass, Electrospray Ionization
D000470:Alkaloids; D004224:Diterpenes