title: Suppression of hepatitis C virus replication by baculovirus vector-mediated short-hairpin RNA expression.
abstract: Short-hairpin RNAs (shRNAs) inhibit gene expression by RNA interference. Here, we report on the inhibition, by baculovirus-based vector-derived shRNAs, of core-protein expression in full-length hepatitis C virus (HCV) replicon cells. shRNAs were designed to target the highly conserved core region of the HCV genome. In particular, the core-shRNA452 containing nucleotides 452-472, as the target in the HCV core gene, dramatically inhibited the expression of the HCV core protein in replicon cells. Furthermore, HCV core-protein expression was inhibited more strongly by the vesicular stomatitis virus glycoprotein (VSV-G)-pseudotyped baculovirus vector than by the wild-type baculovirus vector.
authors.affiliation: Department of Life and Environmental Sciences, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Nobushige
authors.initials: N
authors.lastname: Tamai
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Habu
authors.forename: Myint O O
authors.initials: MO
authors.lastname: Chang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takaku
mesh_terms: D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics*; D016174:Hepacivirus / Q000235:genetics* / Q000502:physiology; D006801:Humans; D017924:Nucleopolyhedroviruses / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014758:Viral Core Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D014758:Viral Core Proteins