Oxidative stress-associated impairment of glucose and ammonia metabolism in the filamentous fungus, Aspergillus niger B1-D.
Oxidative stress events have been shown to be associated with reduced consumption of nutrients in yeasts, but there are very few studies in filamentous fungi. In the present study we investigated the impact of oxidative stress on glucose and ammonia utilization in batch cultures of Aspergillus niger B1-D. The addition of 1mM H(2)O(2) significantly reduced both glucose and ammonia uptake rates in these cultures. Associated with the decreased nutrient uptake, the activity of glyceraldehyde-3-phosphate dehydrogenase was greatly reduced; conversely, the activity of glucose-6-phosphate dehydrogenase remained unchanged. During the period of reduced nutrient uptake, the intracellular ATP and NADPH levels decreased while the amount of trehalose increased. The activities of glutamine synthetase and glutamate dehydrogenase, two key enzymes of ammonia assimilation, remained unchanged in response to H(2)O(2) up to 1mM, suggesting the decreased ammonia uptake rate noted under such conditions is not due to enzyme inactivation caused by oxidative stress, but may be due to an insufficient supply of ATP and NADPH, which are required for ammonia assimilation.
Li
Qiang
Q
Strathclyde Fermentation Centre, Strathclyde Institute of Pharmacy and Biomedical Sciences, Royal College Building, University of Strathclyde, 204 George Street, Glasgow G1 1XW, UK.
Abrashev
Radoslav
R
Harvey
Linda M
LM
McNeil
Brian
B
D000255:Adenosine Triphosphate / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism*; D001234:Aspergillus niger / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D003470:Culture Media; D015536:Down-Regulation; D005947:Glucose / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009249:NADP / Q000378:metabolism; D018384:Oxidative Stress
D003470:Culture Media; D009249:NADP; D000641:Ammonia; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005947:Glucose