title: Cytoprotective roles of senescence marker protein 30 against intracellular calcium elevation and oxidative stress.
abstract: Senescence marker protein 30 (SMP30) is identified as an important aging marker molecule and known to play multifunctional roles as an intracellular calcium regulatory protein in the signaling process. To elucidate the functional significance of SMP30, we established the stably transfected P19 cell line with SMP30 expression vector. Overexpression of SMP30 slightly suppressed the proliferation of P19 cells. However, SMP30 overexpression was cytoprotective against calcium-mediated stress such as calcium ionophore (A23187), and thapsigargin. We found that SMP30 overexpression reduced the elevated intracellular calcium levels induced by A23187, but not by thapsigargin. In addition, SMP30 transfected P19 cells were more protective to tert-butylhydroperoxide induced cytotoxicity, indicating the antioxidative properties of SMP30. Taken together, our results suggest that external calcium regulation and antioxidant properties are involved in the cytoprotective mechanism of SMP30.
authors.affiliation: Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Longevity Life Science and Technology Institutes, Pusan National University, Busan, Korea.
authors.forename: Tae Gen
authors.initials: TG
authors.lastname: Son
authors.forename: So Jung
authors.initials: SJ
authors.lastname: Kim
authors.forename: Keunho
authors.initials: K
authors.lastname: Kim
authors.forename: Min-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Jaewon
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015153:Blotting, Western; D000001:Calcimycin / Q000037:antagonists & inhibitors / Q000633:toxicity; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000502:physiology*; D002273:Carcinogens / Q000037:antagonists & inhibitors / Q000633:toxicity; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D018384:Oxidative Stress / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019284:Thapsigargin / Q000037:antagonists & inhibitors / Q000633:toxicity; D014162:Transfection
chemical_list: D000975:Antioxidants; D002135:Calcium-Binding Proteins; D002273:Carcinogens; D047908:Intracellular Signaling Peptides and Proteins; C513324:Rgn protein, mouse; D000001:Calcimycin; D019284:Thapsigargin; D002118:Calcium