The Arabidopsis AtNPR1 inversely modulates defense responses against fungal, bacterial, or viral pathogens while conferring hypersensitivity to abiotic stresses in transgenic rice.
The nonexpressor of pathogenesis-related (PR) genes (NPR1) protein plays an important role in mediating defense responses activated by pathogens in Arabidopsis. In rice, a disease-resistance pathway similar to the Arabidopsis NPR1-mediated signaling pathway one has been described. Here, we show that constitutive expression of the Arabidopsis NPR1 (AtNPR1) gene in rice confers resistance against fungal and bacterial pathogens. AtNPR1 exerts its protective effects against fungal pathogens by priming the expression of salicylic acid (SA)-responsive endogenous genes, such as the PR1b, TLP (PR5), PR10, and PBZ1. However, expression of AtNPR1 in rice has negative effects on viral infections. The AtNPR1-expressing rice plants showed a higher susceptibility to infection by the Rice yellow mottle virus (RYMV) which correlated well with a misregulation of RYMV-responsive genes, including expression of the SA-regulated RNA-dependent RNA polymerase 1 gene (OsRDR1). Moreover, AtNPR1 negatively regulates the expression of genes playing a role in the plant response to salt and drought stress (rab21, salT, and dip1), which results in a higher sensitivity of AtNPR1 rice to the two types of abiotic stress. These observations suggest that AtNPR1 has both positive and negative regulatory roles in mediating defense responses against biotic and abiotic stresses.
Quilis
Jordi
J
Consorcio CSIC-IRTA Laboratorio de Genética Molecular Vegetal, Jordi Girona 18, Barcelona, Spain.
Peñas
Gisela
G
Messeguer
Joaquima
J
Brugidou
Christophe
C
San Segundo
Blanca
B
D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D015152:Blotting, Northern; D055864:Droughts; D004885:Erwinia / Q000254:growth & development; D005670:Fusarium / Q000254:growth & development; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007113:Immunity, Innate / Q000235:genetics; D020082:Magnaporthe / Q000254:growth & development; D012275:Oryza / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D010940:Plant Proteins / Q000145:classification / Q000235:genetics; D010942:Plant Viruses / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D012324:RNA-Dependent RNA Polymerase / Q000145:classification / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D012965:Sodium Chloride; D012324:RNA-Dependent RNA Polymerase; D020156:Salicylic Acid