title: Eimeria tenella: utilization of a nasal vaccine with sporozoite antigens incorporated into Iscom as protection for broiler breeders against a homologous challenge.
abstract: The aim of this study was to evaluate a nasal vaccine using antigens derived from sporozoites of Eimeria tenella incorporated into Iscom to protect broiler chicks. Forty-five one-day-old chickens (Cobb), unvaccinated against coccidiosis, were used in this experiment. The birds were maintained in separated battery cages and divided into three groups: G1 (n=15), G2 (n=15), and G3 (n=15). G1 received 50 microg of sporozoites+Iscom vaccine, G2 received Iscom without antigens, and G3 received only PBS. The treatments were administered by nasal route on days 0, 7, and 21 of the experiment. On the 28th day, all birds were challenged with 105 sporulated oocysts of E. tenella. On the challenge day, three birds from each group were euthanized to evaluate lymphocyte proliferation. Lesion scores were obtained from five birds from each group, 7 days after challenge. The remaining animals were euthanized on the 50th day. The mean lymphocyte proliferation responses were significantly different (P=0.03); G1 was 2.3-2.6 times more elevated than G2, and G3 (P<0.001). 83% of the birds from G1 showed an IgY antibody reaction by ELISA at challenge. The means for oocysts shedding were 16,890+/-20,511, 48,080+/-50,047, and 65,020+/-74,461, for G1, G2 and G3 birds, respectively. There was no significant difference (P>0.17) in oocysts shedding between groups. However, the G1 and G2 chicks demonstrated reduction in percentage of oocyst shedding when compared to control birds (G3) by 74.02% and 26.05%, respectively. The average lesion scores were G1=0.4, G2=1, and G3=2. This study demonstrated that the lowest lesion score and oocyst shedding were observed in the birds from the group that received antigens derived from sporozoite with an Iscom adjuvant (G1). These results suggest that this vaccine can induce protection against avian coccidiosis.
authors.affiliation: Laboratório de Protozoologia, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina-UEL, Postal Box 6001, 86050-970 Londrina, PR, Brazil. jlgarcia@uel.br
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
authors.forename: José da Silva
authors.initials: Jda S
authors.lastname: Guimarães
authors.forename: Selwyn Arlington
authors.initials: SA
authors.lastname: Headley
authors.forename: Alexey Leon Gomel
authors.initials: AL
authors.lastname: Bogado
authors.forename: Felipe Monteiro
authors.initials: FM
authors.lastname: Bugni
authors.forename: Daniela Cristina
authors.initials: DC
authors.lastname: Ramalho
authors.forename: Leonardo Montemor
authors.initials: LM
authors.lastname: de Souza
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D000953:Antigens, Protozoan / Q000737:chemistry; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D016786:Eimeria tenella / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D016882:ISCOMs / Q000008:administration & dosage / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000469:parasitology / Q000473:pathology; D007700:Kinetics; D008213:Lymphocyte Activation; D011201:Poultry Diseases / Q000469:parasitology / Q000517:prevention & control*; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology; D034101:Sporozoites / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016882:ISCOMs; C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D016052:Protozoan Vaccines; D022223:Vaccines, Subunit