title: Detection of optic nerve head neural canal opening within histomorphometric and spectral domain optical coherence tomography data sets.
abstract: OBJECTIVE
  To assess the ability to detect the neural canal opening (NCO) and its characteristics within three-dimensional (3-D) histomorphometric and 3-D spectral domain optical coherence tomography (SD-OCT) reconstructions of the optic nerve head from nonhuman primate (NHP) eyes.
  
  
  METHODS
  NCO was delineated within 40 radial, sagittal sections of 3-D histomorphometric reconstructions of 44 normal eyes of 38 NHPs, each perfusion fixed at IOP 10 mm Hg, and 3-D SD-OCT (Spectralis; Heidelberg Engineering, Heidelberg, Germany) volumes acquired in vivo from a separate group of 33 normal eyes of 24 NHPs. Within all reconstructions, a least-squares ellipse was fitted to the 80 NCO points. For each eye, the dimensions and plane error (a gauge of planarity) of the fitted ellipse were calculated.
  
  
  RESULTS
  The NCO was successfully delineated within every section of each histomorphometric and SD-OCT reconstruction. Median plane error was similar within histomorphometric and SD-OCT volumes (8 microm, range 4-19, histomorphometry, and 10 microm, range 4-26, SD-OCT) and was small relative to the size of the ellipse. Median histomorphometric ellipse dimensions were 1453 mum (major axis, range 1218-1737) and 1066 microm (minor axis, range 808-1263). Median SD-OCT ellipse dimensions were 1512 microm (major axis, range 1191-1865) and 1060 microm (minor axis, range 772-1248).
  
  
  CONCLUSIONS
  The NCO is biologically continuous and relatively planar within all 3-D histomorphometric and SD-OCT reconstructions. These characteristics support its further evaluation as a reference plane for cross-sectional and longitudinal measurement of optic nerve head structures using 3-D SD-OCT.
authors.affiliation: Optic Nerve Head Research Laboratory, Devers Eye Institute, Legacy Health System, Portland, OR 97208-3950, USA.
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Strouthidis
authors.forename: Hongli
authors.initials: H
authors.lastname: Yang
authors.forename: Brad
authors.initials: B
authors.lastname: Fortune
authors.forename: J Crawford
authors.initials: JC
authors.lastname: Downs
authors.forename: Claude F
authors.initials: CF
authors.lastname: Burgoyne
mesh_terms: D000818:Animals; D001369:Axons; D005260:Female; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008297:Male; D054259:Neural Tube / Q000033:anatomy & histology*; D009898:Optic Disk / Q000033:anatomy & histology*; D041623:Tomography, Optical Coherence / Q000379:methods*