Piezoelectric biosensors assisted with electroacoustic impedance spectroscopy: a tool for accurate quantitative molecular recognition analysis.
In this work, electroacoustic impedance analysis based on a modified Butterworth-Van Dyke (BVD) model is used to complement resonance frequency measurements of piezoelectric crystal sensors for the identification and removal of interfering signals. This approach enables the accurate use of the Sauerbrey correlation to establish a direct relationship between mass deposited at the sensor surface and measured frequency variations. Kinetic models can thus be evaluated and binding constants estimated directly from the measured data. We further demonstrate the usefulness of this approach by applying it to the study of the formation of 11-hydroxy-1-undecanothiol self-assembled monolayers (SAM) as well as to the binding of streptavidin to immobilized biotin. Kinetic and equilibrium parameters were estimated from transient analysis, adsorption isotherms, Scatchard and Hill plots obtained from the frequency data for both the alkanethiol and streptavidin films. This strategy based on electroacoustic impedance assisted quartz-crystal microbalance (QCM) biosensors is expected to be a major contribution for the use of these piezoelectric devices as a reliable and cheap detection system that can easily be integrated into analytical techniques.
Encarnação
João M
JM
IBB-Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine, University of Algarve, 8000-117 Faro, Portugal.
Baltazar
Raul
R
Stallinga
Peter
P
Ferreira
Guilherme N M
GN
D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry*; D017097:Electric Impedance; D005227:Fatty Acids / Q000737:chemistry*; D011791:Quartz / Q000737:chemistry*; D013057:Spectrum Analysis; D019809:Streptavidin / Q000737:chemistry*; D020349:Surface Plasmon Resonance
D005227:Fatty Acids; C016173:undecanoic acid; D011791:Quartz; D001710:Biotin; D019809:Streptavidin