Binding of the Streptococcus gordonii DL1 surface protein Hsa to the host cell membrane glycoproteins CD11b, CD43, and CD50.
Infective endocarditis is frequently attributed to oral streptococci. The mechanisms of pathogenesis, however, are not well understood, although interaction between streptococci and phagocytes are thought to be very important. A highly expressed surface component of Streptococcus gordonii, Hsa, which has sialic acid-binding activity, contributes to infective endocarditis in vivo. In the present study, we found that S. gordonii DL1 binds to HL-60 cells differentiated into monocytes, granulocytes, and macrophages. Using a glutathione S-transferase (GST) fusion to the NR2 domain, which is the sialic acid-binding region of Hsa, we confirmed that the Hsa NR2 domain also binds to differentiated HL-60 cells. To identify which sialoglycoproteins on the surface of differentiated HL-60 cells are receptors for Hsa, intrinsic membrane proteins were assessed by bacterial overlay and far-Western blotting. S. gordonii DL1 adhered to 100- to 150-kDa proteins, a reaction that was abolished by neuraminidase treatment. These sialoglycoproteins were identified as CD11b, CD43, and CD50 by GST pull-down assay and immunoprecipitation with each specific monoclonal antibody. These data suggest that S. gordonii DL1 Hsa specifically binds to three glycoproteins as receptors and that this interaction may be the initial bacterial binding step in infective endocarditis by oral streptococci.
Urano-Tashiro
Yumiko
Y
Department of Microbiology, School of Life Dentistry at Tokyo, Nippon Dental University, Tokyo, Japan. yumiurano@tky.ndu.ac.jp
Yajima
Ayako
A
Takashima
Eizo
E
Takahashi
Yukihiro
Y
Konishi
Kiyoshi
K
D018829:Adhesins, Bacterial / Q000378:metabolism*; D015703:Antigens, CD / Q000378:metabolism*; D026481:Blotting, Far-Western; D039481:CD11b Antigen / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D006389:Hemagglutinins, Viral; D006801:Humans; D047468:Immunoprecipitation; D051917:Leukosialin / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism; D011485:Protein Binding; D012795:Sialoglycoproteins / Q000378:metabolism; D054773:Streptococcus gordonii / Q000502:physiology*
D018829:Adhesins, Bacterial; D015703:Antigens, CD; D039481:CD11b Antigen; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D006389:Hemagglutinins, Viral; C483616:ICAM3 protein, human; C509295:ITGAM protein, human; D051917:Leukosialin; D008565:Membrane Proteins; C465014:SPN protein, human; D012795:Sialoglycoproteins; C450840:hsa protein, Streptococcus gordonii; D009439:Neuraminidase