title: Induced sputum and bronchial mucosal expression of interleukin-13 is not increased in chronic obstructive pulmonary disease.
abstract: BACKGROUND
  The Th2 cytokine interleukin-13 (IL-13) has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). We sought to examine IL-13 expression in COPD subjects in induced sputum and bronchus specimens. We hypothesized that inflammatory cells expressing IL-13 localize to the airway smooth muscle bundle and bronchial glands.
  
  
  METHODS
  Interleukin-13 was measured in sputum samples from subjects with COPD (n = 34) across a range of severity (Global initiative for chronic Obstructive Lung Disease 2-4) and controls (n = 14) using ELISA. IL-13+ cells and inflammatory cells were enumerated within surgically resected proximal airway using immunohistochemical techniques from subjects with COPD (n = 10), smoking (n = 10) and nonsmoking controls (n = 8).
  
  
  RESULTS
  Sputum IL-13 was measurable in only 6/34 subjects with COPD and was not found in the smoking or nonsmoking control subjects. In subjects with COPD and controls there was a paucity of IL-13+ cells. The distribution of inflammatory cells within different airway compartments was similar in COPD and controls except for an increase in CD3(+) lymphocytes within bronchial glands in COPD (P = 0.04).
  
  
  CONCLUSIONS
  Our findings do not support a role for IL-13 in COPD. However, the tissue localization of inflammatory cells to airway compartments, particularly the increase of T cells in glands in COPD may be important in disease.
authors.affiliation: Institute of Lung Health, Leicester, UK.
authors.forename: S
authors.initials: S
authors.lastname: Saha
authors.forename: V
authors.initials: V
authors.lastname: Mistry
authors.forename: R
authors.initials: R
authors.lastname: Siva
authors.forename: D
authors.initials: D
authors.lastname: Parker
authors.forename: R
authors.initials: R
authors.lastname: May
authors.forename: P
authors.initials: P
authors.lastname: Bradding
authors.forename: I D
authors.initials: ID
authors.lastname: Pavord
authors.forename: C E
authors.initials: CE
authors.lastname: Brightling
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D018793:Interleukin-13 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000235:genetics*; D020545:Respiratory Mucosa / Q000378:metabolism*; D012720:Severity of Illness Index; D013183:Sputum / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism*
chemical_list: D018793:Interleukin-13