title: A protein domain-based interactome network for C. elegans early embryogenesis.
abstract: Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.
authors.affiliation: Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. mboxem@partners.org
authors.forename: Mike
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authors.lastname: Boxem
authors.forename: Zoltan
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authors.lastname: Yildirim
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authors.lastname: Cokol
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authors.forename: Anne-Sophie
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authors.lastname: de Smet
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authors.forename: Anne-Lore
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authors.lastname: Schlaitz
authors.forename: Tong
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authors.lastname: Hao
authors.forename: Stuart
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authors.lastname: Milstein
authors.forename: Changyu
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authors.forename: Mike
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authors.lastname: Tipsword
authors.forename: Kevin
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authors.lastname: Drew
authors.forename: Matilde
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authors.forename: Kahn
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authors.lastname: Rhrissorrakrai
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authors.lastname: Drechsel
authors.forename: Daphne
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authors.lastname: Koller
authors.forename: Frederick P
authors.initials: FP
authors.lastname: Roth
authors.forename: Lilia M
authors.initials: LM
authors.lastname: Iakoucheva
authors.forename: A Keith
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authors.lastname: Dunker
authors.forename: Richard
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authors.lastname: Bonneau
authors.forename: Kristin C
authors.initials: KC
authors.lastname: Gunsalus
authors.forename: David E
authors.initials: DE
authors.lastname: Hill
authors.forename: Fabio
authors.initials: F
authors.lastname: Piano
authors.forename: Jan
authors.initials: J
authors.lastname: Tavernier
authors.forename: Sander
authors.initials: S
authors.lastname: van den Heuvel
authors.forename: Anthony A
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authors.lastname: Hyman
authors.forename: Marc
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authors.lastname: Vidal
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology*; D002455:Cell Division; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D020543:Proteome; D020798:Two-Hybrid System Techniques
chemical_list: D020543:Proteome