Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells.
OBJECTIVE
To examine the effect of the ovarian steroids on the gene expression profile in human uterine microvascular endothelial cells.


METHODS
An experimental study.


METHODS
University hospital and research laboratory.


METHODS
Eighteen premenopausal women with proven fertility undergoing hysterectomy for cervical carcinoma in situ or cervical dysplasia.


METHODS
None.


METHODS
The effect of E(2) and P on gene expression profile in pooled human uterine microvascular endothelial cells was examined with cDNA microarray analysis and confirmed with quantitative real-time reverse transcriptase polymerase chain reaction. In vivo endometrial endothelial expression of the proteins encoded by the genes significantly regulated by E(2) and/or P was examined with Western blotting analysis and immunohistochemistry.


RESULTS
The genes steadily up-regulated by E(2) and/or P included angiogenic factors, water transporters, immunomodulators, binding proteins, electron transporters, amino acid transporter, signal transduction proteins, and blood pressure regulation factors. The proteins encoded by IL1RL1, WAS, and NPPA were detected in endometrial microvascular endothelial cells.


CONCLUSIONS
Progesterone (alone or in combination with E(2)) can induce the genes involved in angiogenesis, edematous change, and leukocyte recruitment in human uterine microvascular endothelial cells. Ovarian steroids may contribute to endometrial differentiation via the action on local microvessels.
Yasuo
Tadahiro
T
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Kitaya
Kotaro
K
D000328:Adult; D015232:Dinoprostone / Q000008:administration & dosage*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D012739:Gonadal Steroid Hormones / Q000008:administration & dosage; D006801:Humans; D055806:Microvessels / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D011374:Progesterone / Q000008:administration & dosage*; D020543:Proteome / Q000378:metabolism*; D014599:Uterus / Q000098:blood supply* / Q000166:cytology / Q000378:metabolism*
D012739:Gonadal Steroid Hormones; D020543:Proteome; D011374:Progesterone; D015232:Dinoprostone