title: Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems.
abstract: We describe the development of a novel PCR assay for the rapid detection of members of the Brucella genus, and the differentiation between six recognized Brucella species. The assay has proven to be highly specific with the additional advantage of being suitable for use with both conventional and real-time PCR.
authors.affiliation: National Centre for Zoonoses, Bacterial Animal Diseases and Antimicrobial Resistance (ZOBA), Institute of Veterinary Bacteriology, University of Bern, Vetsuisse Faculty, Länggass-Strasse 122, P. O. Box, CH-3001 Bern, Switzerland.
authors.forename: V
authors.initials: V
authors.lastname: Hinić
authors.forename: I
authors.initials: I
authors.lastname: Brodard
authors.forename: A
authors.initials: A
authors.lastname: Thomann
authors.forename: Z
authors.initials: Z
authors.lastname: Cvetnić
authors.forename: P V
authors.initials: PV
authors.lastname: Makaya
authors.forename: J
authors.initials: J
authors.lastname: Frey
authors.forename: C
authors.initials: C
authors.lastname: Abril
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002002:Brucella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002003:Brucella abortus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D041002:Brucella canis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017347:Brucella melitensis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D042061:Brucella ovis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D041021:Brucella suis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002006:Brucellosis / Q000382:microbiology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004285:Dogs; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial