title: Inhibition of the wine spoilage yeast Dekkera bruxellensis by bovine lactoferrin-derived peptides.
abstract: The antimicrobial action of lactoferrin (LF)-derived peptides against Dekkera bruxellensis strains isolated from spoiled wines has been examined. The study included a fifteen-residue peptide (LfcinB(17-31)) derived from bovine lactoferricin B and a bovine LF pepsin hydrolysate (LFH). In vitro assays showed the inhibitory properties of LfcinB(17-31) on D. bruxellensis growth with IC(50) and MIC values in the micromolar range. Strains tested showed different sensitivity to the peptide. LfcinB(17-31) showed fungicidal properties towards all strains tested in laboratory growth medium. However, the extent of fungicidal activity was strain-dependent in must and wine, confirming the different antimicrobial action of peptides depending on both the food matrix and the target micro-organism. The binding of LfcinB(17-31) to D. bruxellensis cells was visualized by fluorescence microscopy and correlated with the fungicidal activity in the different matrixes. LfcinB(17-31) and LFH showed growth inhibitory properties in wine suggesting their potential use for spoilage control.
authors.affiliation: Departamento de Biotecnología de Alimentos, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Valencia, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Enrique
authors.forename: Jose F
authors.initials: JF
authors.lastname: Marcos
authors.forename: María
authors.initials: M
authors.lastname: Yuste
authors.forename: Mireia
authors.initials: M
authors.lastname: Martínez
authors.forename: Salvador
authors.initials: S
authors.lastname: Vallés
authors.forename: Paloma
authors.initials: P
authors.lastname: Manzanares
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002417:Cattle; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D055167:Dekkera / Q000187:drug effects* / Q000254:growth & development; D005506:Food Contamination / Q000517:prevention & control; D007781:Lactoferrin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010446:Peptide Fragments / Q000494:pharmacology*; D014920:Wine / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D010446:Peptide Fragments; C082171:lactoferricin B; D007781:Lactoferrin