title: Glycosylphosphatidylinositol-anchored arginine-specific ADP-ribosyltransferase7.1 (Art7.1) on chicken B cells: the possible role of Art7 in B cell receptor signalling and proliferation.
abstract: Arginine-specific ADP-ribosyltransferase (Art) catalyzes the mono-ADP-ribosylation, in which it transfers a single ADP-ribose moiety of NAD to the arginine residue(s) of target proteins, and may regulate the function of the proteins or peptides in cellular processes. In vertebrates, Art family is consisted of seven members (Arts1-7), and these Arts are distributed among various tissues except B lymphocytes. Previously, we described molecular cloning, characterization and distribution of glycosylphosphatidylinositol (GPI)-anchored Arts, Art7.1 and Art7.2 (formerly, we referred as cgArt1 and cgArt2, respectively) in chicken tissues (Terashima et al (2005) Biochem J 389:853-861). Here, we demonstrate for the first time that Art7.1 was predominantly expressed on the surface of B cells from the bursa of Fabricius as a GPI-anchored form, as well as on T cells from the thymocytes. Furthermore, we show that the expression of Art7.1 molecules on B cells could modulate the B cell receptor (BCR) signalling and direct the B cell fate to maturation. Thus, our present observation sheds light on the Art molecule expressed on B cells and its possible functional role in BCR signalling.
authors.affiliation: Department of Biochemistry, Shimane University Faculty of Medicine, Izumo 693-8501, Japan. mtera@med.shimane-u.ac.jp
authors.forename: Masaharu
authors.initials: M
authors.lastname: Terashima
authors.forename: Mai
authors.initials: M
authors.lastname: Takahashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimoyama
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Tanigawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Urano
authors.forename: Mikako
authors.initials: M
authors.lastname: Tsuchiya
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D002060:Bursa of Fabricius / Q000166:cytology; D049109:Cell Proliferation; D002645:Chickens; D017261:Glycosylphosphatidylinositols / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013601:T-Lymphocytes / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D017261:Glycosylphosphatidylinositols; D007527:Isoenzymes; D011947:Receptors, Antigen, B-Cell; D036002:ADP Ribose Transferases