title: Canonical Wnt signalling induces satellite-cell proliferation during adult skeletal muscle regeneration.
abstract: Satellite cells represent the stem cell population of adult skeletal muscle. The molecular mechanisms that control the proliferation of satellite cells are not well understood. In this study, we show that in response to injury, myofibres activate Wnt ligand transcription and activate a reporter cell line that is sensitive to the canonical Wnt-signalling pathway. Activated satellite cells on isolated cultured myofibres show robust expression of activated-beta-catenin (Act-beta-Cat), a key downstream transcriptional coactivator of canonical Wnt signalling. We provide evidence that the Wnt family of secreted glycoproteins act on satellite cells in a ligand-specific manner. Overexpression of Wnt1, Wnt3a or Wnt5a protein causes a dramatic increase in satellite-cell proliferation. By contrast, exposure of satellite cells to Wnt4 or Wnt6 diminishes this process. Moreover, we show that the prolonged satellite-cell quiescence induced by inhibitory Wnt is reversible and exposing inhibited satellite cells to stimulatory Wnt signalling restores their proliferation rate. Stimulatory Wnt proteins induce premature satellite cell BrdU incorporation as well as nuclear translocation of Act-beta-Cat. Finally, we provide evidence that the Act-beta-Cat translocation observed in single fibres during in vitro culture also occurs in cases of acute and chronic skeletal muscle regeneration in rodents and humans. We propose that Wnt proteins may be key factors that regulate the rate of satellite-cell proliferation on adult muscle fibres during the wound-healing response.
authors.affiliation: School of Biological Sciences, AMS Building, University of Reading, Whiteknights, PO Box 228, Reading, Berkshire RG6 6AJ, UK.
authors.forename: Anthony
authors.initials: A
authors.lastname: Otto
authors.forename: Corina
authors.initials: C
authors.lastname: Schmidt
authors.forename: Graham
authors.initials: G
authors.lastname: Luke
authors.forename: Steve
authors.initials: S
authors.lastname: Allen
authors.forename: Petr
authors.initials: P
authors.lastname: Valasek
authors.forename: Francesco
authors.initials: F
authors.lastname: Muntoni
authors.forename: Diana
authors.initials: D
authors.lastname: Lawrence-Watt
authors.forename: Ketan
authors.initials: K
authors.lastname: Patel
mesh_terms: D000328:Adult; D000818:Animals; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D002469:Cell Separation; D002675:Child, Preschool; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D008810:Mice, Inbred C57BL; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000502:physiology*; D041681:NIH 3T3 Cells; D051381:Rats; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology*; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D051153:Wnt Proteins; D051176:beta Catenin