title: Evaluation of a microplate agglutination test (MAT) for serological diagnosis of canine brucellosis.
abstract: A microplate agglutination test (MAT) was compared with the tube agglutinin test (TAT), a standard test for the diagnosis of Brucella canis, in terms of the sensitivity and specificity. The results showed that MAT was more sensitive, simpler to perform and easier to read the results than TAT. On top of that the MAT allows us to handle a larger number of samples at once. Using this method we conducted sero-surveillance of the prevalence of B. canis in dogs kept in an Animal Shelter located in Kanagawa Prefecture. Twelve of 485 (2.5%) showed seropositive against B. canis. These results indicate that B. canis infection in dogs is still occurring in Japan.
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kimura
authors.forename: Koichi
authors.initials: K
authors.lastname: Imaoka
authors.forename: Michio
authors.initials: M
authors.lastname: Suzuki
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Kamiyama
authors.forename: Akio
authors.initials: A
authors.lastname: Yamada
mesh_terms: D000372:Agglutination Tests / Q000379:methods / Q000662:veterinary*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D041002:Brucella canis / Q000302:isolation & purification*; D002006:Brucellosis / Q000097:blood / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004196:Disease Outbreaks / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D004285:Dogs; D005260:Female; D007564:Japan / Q000453:epidemiology; D008297:Male; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D018709:Statistics, Nonparametric
chemical_list: D000907:Antibodies, Bacterial