Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA.
Spectrofluorometric titration, electrospray ionization time-of-flight mass spectrometric and UV melting methods were employed to study the binding of chelerythrine and sanguinarine to bulged DNA. The results showed that both alkaloids bind specifically to single pyrimidine (C, T) bulge sites. The ability of sanguinarine to bind to both regular and bulged hairpins was found to be stronger than that of chelerythrine, but the binding selectivity of chelerythrine toward single-base bulges was much larger than that of sanguinarine.
Bai
Li-Ping
LP
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
Cai
Zongwei
Z
Zhao
Zhong-Zhen
ZZ
Nakatani
Kazuhiko
K
Jiang
Zhi-Hong
ZH
D020029:Base Pairing; D053119:Benzophenanthridines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007546:Isoquinolines / Q000737:chemistry*; D015394:Molecular Structure; D011743:Pyrimidines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D017186:Titrimetry; D044366:Transition Temperature
D053119:Benzophenanthridines; D007546:Isoquinolines; D011743:Pyrimidines; D004247:DNA; C005705:sanguinarine; C016299:chelerythrine; C030986:pyrimidine