Non-parametric estimation of posterior error probabilities associated with peptides identified by tandem mass spectrometry.
BACKGROUND
A mass spectrum produced via tandem mass spectrometry can be tentatively matched to a peptide sequence via database search. Here, we address the problem of assigning a posterior error probability (PEP) to a given peptide-spectrum match (PSM). This problem is considerably more dif.cult than the related problem of estimating the error rate associated with a large collection of PSMs. Existing methods for estimating PEPs rely on a parametric or semiparametric model of the underlying score distribution.


RESULTS
We demonstrate how to apply non-parametric logistic regression to this problem. The method makes no explicit assumptions about the form of the underlying score distribution; instead, the method relies upon decoy PSMs, produced by searching the spectra against a decoy sequence database, to provide a model of the null score distribution. We show that our non-parametric logistic regression method produces accurate PEP estimates for six different commonly used PSM score functions. In particular, the estimates produced by our method are comparable in accuracy to those of PeptideProphet, which uses a parametric or semiparametric model designed speci.cally to work with SEQUEST. The advantage of the non-parametric approach is applicability and robustness to new score functions and new types of data.


BACKGROUND
C++ code implementing the method as well as supplementary information is available at http://noble.gs. washington.edu/proj/qvality
Käll
Lukas
L
Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Storey
John D
JD
Noble
William Stafford
WS
D000465:Algorithms; D000595:Amino Acid Sequence; D016477:Artifacts; D003198:Computer Simulation; D003627:Data Interpretation, Statistical; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D015233:Models, Statistical; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020539:Sequence Analysis, Protein / Q000379:methods*
D010455:Peptides