Photopolymerization as an innovative detection technique for low-density microarrays.
One limitation that accounts in part for the scarcity of commercially available diagnostic microarrays is the expense associated with fluorescence detection. Here we present a colorimetric method based on photopolymerization as an "on-chip" signal amplification technique. Proof of principle experiments are detailed and followed by the use of a simple influenza microarray to demonstrate the technique for the first time with clinical samples. The advantages of this new technique include rapid (<5 min) signal amplification ( approximately 105) in ambient conditions for both DNA and protein microarrays, low reagent cost (<$1 per assay), visual or inexpensive detection, and signal preservation for at least two years under ambient conditions.
Kuck
Laura R
LR
InDevR, Inc., 2100 Central Ave., Suite 106, Boulder CO 80301, USA. lkuck@indevr.net
Taylor
Amber W
AW
D003124:Colorimetry; D005453:Fluorescence; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009975:Orthomyxoviridae / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*
D011108:Polymers