Polymerase chain reaction-based biochemical logic gate coupled with cell-free transcription-translation of green fluorescent protein as a report gate.
Polymerase chain reaction-based biochemical logic gates were designed for AND, OR, NOT, and AND-NOT operations, whose output signal is reported by coupled cell-free transcription-translation of green fluorescent protein.
Nojima
Takahiko
T
Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo, Komaba 4-6-1, Meguro-ku, Tokyo 153-8505, Japan. nojima@iis.u-tokyo.ac.jp
Yamamoto
Takatoki
T
Kimura
Hiroshi
H
Fujii
Teruo
T
D002474:Cell-Free System; D004247:DNA; D049452:Green Fluorescent Proteins / Q000235:genetics*; D044085:Microfluidics; D014176:Protein Biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic / Q000502:physiology*
D049452:Green Fluorescent Proteins; D004247:DNA