Phosphoesterase activity of polyoxomolybdates: diffusion ordered NMR spectroscopy as a tool for obtaining insights into the reactivity of polyoxometalate clusters.
Diffusion ordered NMR spectroscopy (DOSY NMR) is shown to be an excellent tool for observing reactive transients in the hydrolysis of the phosphatase model substrate (p-nitrophenyl)phosphate (NPP) promoted by polyoxomolybdate.
Lokeren
Luk Van
LV
Vrije Universiteit Brussels, High Resolution NMR centrum, Pleinlaan 2, B-1050 Brussels, Belgium.
Cartuyvels
Els
E
Absillis
Gregory
G
Willem
Rudolph
R
Parac-Vogt
Tatjana N
TN
D004058:Diffusion; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008982:Molybdenum / Q000737:chemistry* / Q000378:metabolism; D009596:Nitrophenols / Q000737:chemistry* / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000737:chemistry* / Q000378:metabolism; D010087:Oxides / Q000737:chemistry* / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000378:metabolism
D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010087:Oxides; C527543:PM-17 polyoxomolybdate; C008644:nitrophenylphosphate; D008982:Molybdenum; D010744:Phosphoric Monoester Hydrolases