Tetronic-oligolactide-heparin hydrogel as a multi-functional scaffold for tissue regeneration.
A novel cell-supporting scaffold, Tetronic-oligolactide-heparin (TLH) hydrogel, was prepared by coupling heparin to polymerized Tetronic-oligolactide for use in improving tissue regeneration. Aqueous TLH solutions showed thermosensitive behavior, demonstrating potential for use as injectable hydrogels. The content and activity of conjugated heparin were determined to be 61 wt.-% of total polymer and 67.2% of intact heparin activity, respectively. The basic fibroblast growth factor (bFGF) binding assay showed TLH hydrogel had a relatively high bFGF affinity, which indicates applicability for growth factor delivery. Chondrocyte culture on hydrogels revealed that the cell viability and the amount of synthesized glycosaminoglycan for TLH hydrogel were higher than those for alginate gel.
Go
Dong Hyun
DH
Department of Molecular Science and Technology, Ajou University, Suwon, Republic of Korea.
Joung
Yoon Ki
YK
Lee
Sang Young
SY
Lee
Myung Chul
MC
Park
Ki Dong
KD
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004146:Dioxanes / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006493:Heparin / Q000737:chemistry*; D020100:Hydrogels; D009682:Magnetic Resonance Spectroscopy; D010314:Partial Thromboplastin Time; D012038:Regeneration; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering
D004146:Dioxanes; D020100:Hydrogels; D016222:Fibroblast Growth Factor 2; D006493:Heparin; C091880:dilactide