title: Legume anchor markers link syntenic regions between Phaseolus vulgaris, Lotus japonicus, Medicago truncatula and Arachis.
abstract: We have previously described a bioinformatics pipeline identifying comparative anchor-tagged sequence (CATS) loci, combined with design of intron-spanning primers. The derived anchor markers defining the linkage position of homologous genes are essential for evaluating genome conservation among related species and facilitate transfer of genetic and genome information between species. Here we validate this global approach in the common bean and in the AA genome complement of the allotetraploid peanut. We present the successful conversion of approximately 50% of the bioinformatics-defined primers into legume anchor markers in bean and diploid Arachis species. One hundred and four new loci representing single-copy genes were added to the existing bean map. These new legume anchor-marker loci enabled the alignment of genetic linkage maps through corresponding genes and provided an estimate of the extent of synteny and collinearity. Extensive macrosynteny between Lotus and bean was uncovered on 8 of the 11 bean chromosomes and large blocks of macrosynteny were also found between bean and Medicago. This suggests that anchor markers can facilitate a better understanding of the genes and genetics of important traits in crops with largely uncharacterized genomes using genetic and genome information from related model plants.
authors.affiliation: Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, DK-8000, Aarhus C, Denmark.
authors.forename: Birgit Kristine
authors.initials: BK
authors.lastname: Hougaard
authors.forename: Lene Heegaard
authors.initials: LH
authors.lastname: Madsen
authors.forename: Niels
authors.initials: N
authors.lastname: Sandal
authors.forename: Marcio
authors.initials: M
authors.lastname: de Carvalho Moretzsohn
authors.forename: Jakob
authors.initials: J
authors.lastname: Fredslund
authors.forename: Leif
authors.initials: L
authors.lastname: Schauser
authors.forename: Anna Marie
authors.initials: AM
authors.lastname: Nielsen
authors.forename: Trine
authors.initials: T
authors.lastname: Rohde
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.forename: David John
authors.initials: DJ
authors.lastname: Bertioli
authors.forename: Jens
authors.initials: J
authors.lastname: Stougaard
mesh_terms: D010367:Arachis / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D000070116:Lotus / Q000235:genetics*; D046913:Medicago truncatula / Q000235:genetics*; D027805:Phaseolus / Q000235:genetics*; D026801:Synteny
chemical_list: D005819:Genetic Markers