Suppression of hepatitis C virus replication by baculovirus vector-mediated short-hairpin RNA expression.
Short-hairpin RNAs (shRNAs) inhibit gene expression by RNA interference. Here, we report on the inhibition, by baculovirus-based vector-derived shRNAs, of core-protein expression in full-length hepatitis C virus (HCV) replicon cells. shRNAs were designed to target the highly conserved core region of the HCV genome. In particular, the core-shRNA452 containing nucleotides 452-472, as the target in the HCV core gene, dramatically inhibited the expression of the HCV core protein in replicon cells. Furthermore, HCV core-protein expression was inhibited more strongly by the vesicular stomatitis virus glycoprotein (VSV-G)-pseudotyped baculovirus vector than by the wild-type baculovirus vector.
Suzuki
Hitoshi
H
Department of Life and Environmental Sciences, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
Tamai
Nobushige
N
Habu
Yuichiro
Y
Chang
Myint O O
MO
Takaku
Hiroshi
H
D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics*; D016174:Hepacivirus / Q000235:genetics* / Q000502:physiology; D006801:Humans; D017924:Nucleopolyhedroviruses / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014758:Viral Core Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
D034741:RNA, Small Interfering; D014758:Viral Core Proteins