Inhibition of the wine spoilage yeast Dekkera bruxellensis by bovine lactoferrin-derived peptides.
The antimicrobial action of lactoferrin (LF)-derived peptides against Dekkera bruxellensis strains isolated from spoiled wines has been examined. The study included a fifteen-residue peptide (LfcinB(17-31)) derived from bovine lactoferricin B and a bovine LF pepsin hydrolysate (LFH). In vitro assays showed the inhibitory properties of LfcinB(17-31) on D. bruxellensis growth with IC(50) and MIC values in the micromolar range. Strains tested showed different sensitivity to the peptide. LfcinB(17-31) showed fungicidal properties towards all strains tested in laboratory growth medium. However, the extent of fungicidal activity was strain-dependent in must and wine, confirming the different antimicrobial action of peptides depending on both the food matrix and the target micro-organism. The binding of LfcinB(17-31) to D. bruxellensis cells was visualized by fluorescence microscopy and correlated with the fungicidal activity in the different matrixes. LfcinB(17-31) and LFH showed growth inhibitory properties in wine suggesting their potential use for spoilage control.
Enrique
María
M
Departamento de Biotecnología de Alimentos, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Valencia, Spain.
Marcos
Jose F
JF
Yuste
María
M
Martínez
Mireia
M
Vallés
Salvador
S
Manzanares
Paloma
P
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002417:Cattle; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D055167:Dekkera / Q000187:drug effects* / Q000254:growth & development; D005506:Food Contamination / Q000517:prevention & control; D007781:Lactoferrin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010446:Peptide Fragments / Q000494:pharmacology*; D014920:Wine / Q000382:microbiology*
D000935:Antifungal Agents; D010446:Peptide Fragments; C082171:lactoferricin B; D007781:Lactoferrin