title: Nuclear localization of profilin III-ArpM1 complex in mouse spermiogenesis.
abstract: Actin-related proteins (Arps) have been reported to be localized in the cell nucleus, and implicated in the regulation of chromatin and nuclear structure, as well as being involved in cytoplasmic functions. We demonstrate here that mouse ArpM1, which closely resembles the conventional actin, is expressed exclusively in the testis, particularly in haploid germ cells. ArpM1 protein first appears in the round spermatid and changes its localization dynamically in the nucleus during spermiogenesis. By co-immunoprecipitation analysis, profilin III was identified as ArpM1-interacting protein. Our findings suggest that the testis-specific profilin III-ArpM1 complex may be involved in conformational changes in the organization of the sperm-specific nucleus.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hara
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamagata
authors.forename: Kei
authors.initials: K
authors.lastname: Oguchi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Baba
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051304:Profilins / Q000378:metabolism*; D011487:Protein Conformation; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000199:Actins; C532569:Actrt3 protein, mouse; D051304:Profilins