A novel hairless mouse model on an atopic dermatitis-prone genetic background generated by receptor-mediated transgenesis.
Current mouse models for atopic dermatitis (AD) have a serious drawback, being the existence of dense hair on the body. Thus, a hairless animal model on an AD-prone genetic background will be a powerful tool to investigate the basis of and therapy for this complex disease. We applied the Toxin Receptor-mediated Cell Knockout (TRECK) method to generate a hairless transgenic (Tg) mice on the NC/Nga background, an AD-prone inbred strain. A minigene with the mouse Keratin71 (Krt71) promoter and human diphtheria toxin receptor, which intrinsically functions as the heparin-binding EGF-like growth factor, was introduced into the pronucleus of NC/Nga oocytes. Unexpectedly NCN24, one NC/Nga Tg line, showed a dominant hairless phenotype without diphtheria toxin administration. Furthermore, the atopic dermatitis-like predisposition and IgE elevation was observed in both NCN24 and the NC/Nga wildtype strain. NCN24 mice, which we have newly developed, will be useful to assess drugs for AD therapy, being able to monitor skin inflammation without shaving.
Takada
Toyoyuki
T
Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 3-18-22, Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan.
Shitara
Hiroshi
H
Matsuoka
Kunie
K
Kojima
Erika
E
Ishii
Rie
R
Kikkawa
Yoshiaki
Y
Taya
Choji
C
Karasuyama
Hajime
H
Kohno
Kenji
K
Yonekawa
Hiromichi
H
D000818:Animals; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000276:immunology; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D051379:Mice; D008812:Mice, Hairless; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic
C587883:HBEGF protein, human; C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D007073:Immunoglobulin E