Polysaccharides from Gracilaria corticata: sulfation, chemical characterization and anti-HSV activities.
In this study, we have analyzed water-extracted polysaccharides of Gracilaria corticata. The water extract (WE), a galactan-containing sub-fraction (F3) and their hyper sulfated derivatives (WES1, WES2, F3S1 and F3S2) had anti-HSV activity with inhibitory concentration 50% (IC50) from 1.1 to 27.4 microg/ml. Sub-fraction F3, which has a molecular mass of 30 kDa, consists of a backbone of beta-(1-->3) and alpha-(1-->4)-linked-galactopyranosyl residues. This linear galactan contained Gal2Xyl1, Gal2AnGal2, Gal4 and Me-Gal3AnGal2 as oligomeric building subunits. Sulfate group was located at C-4 of (1-->3)-linked galactopyranosyl residues of the native galactan, and appeared to be very important for the anti-herpetic activity.
Chattopadhyay
Kausik
K
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, WB 713 104, India.
Ghosh
Tuhin
T
Pujol
Carlos A
CA
Carlucci
María J
MJ
Damonte
Elsa B
EB
Ray
Bimalendu
B
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D002850:Chromatography, Gel; D045424:Complex Mixtures; D005685:Galactans / Q000737:chemistry; D044724:Gracilaria / Q000737:chemistry*; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D006868:Hydrolysis / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D010504:Periodic Acid / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013431:Sulfates / Q000378:metabolism*; D014709:Vero Cells
D000925:Anticoagulants; D000998:Antiviral Agents; D045424:Complex Mixtures; D005685:Galactans; D011134:Polysaccharides; D013431:Sulfates; D010504:Periodic Acid; C009288:metaperiodate