Sample preparation method for plasma membrane proteome analysis.
The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.
Park
Seung-Ah
SA
School of Life Sciences and Biotechnology, Korea University, 1, 5-ga Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
Kim
Mi-Ryung
MR
Kim
Pan-Kyeom
PK
Cho
Hyung-Don
HD
Han
Gi-Yeon
GY
Kim
Chan-Wha
CW
D001798:Blood Proteins / Q000032:analysis*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome; D014461:Ultracentrifugation
D001798:Blood Proteins; D020543:Proteome