[Expression of toll-like receptors in human epidermal keratinocytes].
OBJECTIVE
To investigate the toll-like receptors (TLR) profile of human epidermal keratinocytes.


METHODS
We cultured the immortalized human epidermal keratinocyte cell line HaCaT cells and normal human epidermal keratinocytes (NHEK) and separated epidermis with dispase from foreskins. TLR 1-10 mRNA expression was detected with reverse transcription polymerase chain reaction (RT-PCR). TLR 2 and 4 protein expressions on surface of HaCaT cells and NHEK were detected using flow cytometry.


RESULTS
HaCaT cells, NHEK, and epidermis all expressed TLR 1-10 mRNA with different intensity. TLR 4 protein was detected on the surfaces of HaCaT cells and NHEK, while the expression of TLR 2 protein was few.


CONCLUSIONS
Human epidermal keratinocytes constitutively express all TLR 1-10 mRNA, which may enable human keratinocytes to respond to a wide range of pathogenic micro-organisms.
Liu
Su-Jun
SJ
Department of Clinical Dermatology, Institute of Dermatology, CAMS and PUMC, Nanjing 210042, China.
Zhang
Cai-Ping
CP
Zhou
Wu-Qing
WQ
Chen
Min
M
Lin
Lin
L
D002460:Cell Line; D045744:Cell Line, Tumor; D000078404:Epidermal Cells; D005434:Flow Cytometry; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*
D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors