title: [Human bone marrow stromal cells facilitate the cord blood CD34+ cells ex vivo expansion and short-term engraftment in NOD/SCID mice].
abstract: OBJECTIVE
  To explore the potential of human bone marrow stromal cells (MSCs) as the feeding-layer to promote ex vivo expansion of cord blood CD34+ cells and engraftment of the expanded cells in NOD/SCID mice.
  
  
  METHODS
  Human MSCs were routinely isolated and cultured. MSCs at passage 3 were used as feeding-layer for the expansion of cord blood CD34+ cell in the presence of thrombopoietin (TPO), flt3/flk2 ligand (FL), stem cell factor (SCF) and granulocyte-colony stimulating factor (G-CSF). The engraftment potential between unexpanded and expanded cord blood cells transplanted into NOD/SCID mice was compared.
  
  
  RESULTS
  The total nucleated cells (TNC), CD34 cells and colony forming units (CFUs) in the MSC feeding culture were increased by 111.6-, 19.3- and 58-fold after 1 week expansion and 532.8-, 41.3- and 563.5- fold increased after 2 weeks expansion respectively as compared with that in non MSC feeding culture. In transplant experiment, the percentage of human CD45+ cells (45.3% -59.1%) in bone marrow of recipient mice transplanted with the MSC feeding expanded cells was the highest in all the groups at six weeks after transplantation.
  
  
  CONCLUSIONS
  Human MSCs enhance CB CD34+ cells in vitro expansion and their capacity of short-term engraftment in NOD/SCID mice.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Fei
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Wu
authors.forename: Zai
authors.initials: Z
authors.lastname: Chang
authors.forename: Kou-Rong
authors.initials: KR
authors.lastname: Miao
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Zhou
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Pan
authors.forename: Cheng-Ya
authors.initials: CY
authors.lastname: Wang
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D002469:Cell Separation; D002478:Cells, Cultured; D036101:Cord Blood Stem Cell Transplantation; D005312:Fetal Blood / Q000166:cytology*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor