Beneficial effects of carnosic acid on dieldrin-induced dopaminergic neuronal cell death.
Carnosic acid (CA) is one of the bioactive polyphenols present in extracts of the herb rosemary (Rosmarinus officinalis). In this study, we examined possible protective effects of CA on neurotoxicity induced by dieldrin, an organochlorine pesticide implicated in sporadic Parkinson's disease, in cultured dopaminergic cells (SN4741). CA (5-10 muM) pretreatment showed potent protective effects in a concentration-related manner and prevented dieldrin (10 muM)-induced caspase-3 activation, Jun N-terminal kinase phosphorylation, and caspase-12 activation. Furthermore, dieldrin-induced downregulation of brain-derived neurotrophic factor production was significantly attenuated by CA. These results suggest that CA may safeguard dopaminergic neuronal cells from environmental neurotoxins by enhancing brain-derived neurotrophic factor and repressing apoptotic molecules.
Park
Jeong Ae
JA
Department of Biotechnology (BK21 Program), College of Dentistry (BK21 Program), Chosun University, 375 Seosuk-dong, Gwangju, Republic of Korea.
Kim
Seung
S
Lee
Sook-Young
SY
Kim
Chun-Sung
CS
Kim
Do Kyung
DK
Kim
Sung-Jun
SJ
Chun
Hong Sung
HS
D045784:Abietanes / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D053454:Caspase 12 / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D004026:Dieldrin / Q000633:toxicity*; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D027542:Rosmarinus / Q000737:chemistry
D045784:Abietanes; D000975:Antioxidants; D019208:Brain-Derived Neurotrophic Factor; D010936:Plant Extracts; D048031:JNK Mitogen-Activated Protein Kinases; D053454:Caspase 12; D053148:Caspase 3; D004026:Dieldrin; C018381:salvin; D004298:Dopamine