TGF-beta expression by allogeneic bone marrow stromal cells ameliorates diabetes in NOD mice through modulating the distribution of CD4+ T cell subsets.
BMSCs could promote the regeneration of islet beta-cell, but the status of BMSCs under diabetes is still unknown. Our study verified the effect of allogeneic BMSCs (ICR) transferred into NOD mice on blood glucose and CD4+ T cells subsets function. In vivo experiment, BMSCs could decrease blood glucose, weaken lymphocytes proliferation. In vitro experiment, the distribution of CD4+ T cell subsets was changed after co-culture with BMSCs, resulting in a greater frequency of Treg cells and reduced representation of Th17 cells. After TGF-beta blockade, CD4+ T cells differentiated along a route favoring development of Th17, but not Treg cells. Thus, NOD can be treated by BMSCs which changes the distribution of CD4+ T cells, increases the number of Treg cells, and inhibits the differentiation of Th17 cells. And the positive effects of allogeneic BMSCs in the treatment of NOD mice depend on the regulation of TGF-beta secreted by BMSCs.
Zhao
Wei
W
Department of Neurobiology, Harbin Medical University Provincial Key Lad of Neurobiology, 194 XueFu Road, Harbin, Heilongjiang 150081, China.
Wang
Yan
Y
Wang
Dandan
D
Sun
Bo
B
Wang
Guangyou
G
Wang
Jinghua
J
Kong
Qingfei
Q
Wang
Qi
Q
Peng
Haisheng
H
Jin
Lianhong
L
Li
Hulun
H
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D016026:Bone Marrow Transplantation; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000276:immunology*; D005260:Female; D007328:Insulin / Q000276:immunology; D007515:Islets of Langerhans / Q000166:cytology / Q000276:immunology; D051379:Mice; D016688:Mice, Inbred NOD; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000276:immunology*; D014184:Transplantation, Homologous
D016207:Cytokines; D007328:Insulin; D016212:Transforming Growth Factor beta