Using aptamers to study protein-protein interactions.
The emerging science of systems biology focuses on the systematic study of complex interactions in whole biological systems. A systemic, or integrative, methodology is employed as the chief means of discovering new properties and understanding the aggregate of processes that occur in a biological system. Accordingly, the Human Genome Project has provided a complete map of genes and resultant proteins corresponding to their function. Protein-protein interactions are important pieces of this biological tapestry, and understanding how they work cooperatively in a cell will result in a better understanding of the whole organism. To accomplish this objective, we report the use of DNA/RNA aptamers as a novel tool for the study and elucidation of protein-protein interactions, both in vivo and in vitro.
Parekh
Parag
P
Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, Center for Research at the Bio/Nano Interface, University of Florida, Gainesville, FL 32611-7200, USA.
Martin
Jennifer
J
Chen
Yan
Y
Colon
Dalia
D
Wang
Hui
H
Tan
Weihong
W
D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*
D052157:Aptamers, Nucleotide