An extracellular galactoxylomannan of acapsular Cryptococcus laurentii mutant.
An extracellular galactoxylomannan (GalXM) composed of D-Gal (34.0%), D-Xyl (26.6%) and D-Man (31.0%), and a small amount of L-Ara (4.0%) and D-Glc (4.4%) has been isolated from culture medium of acapsulated mutant of Cryptococcus laurentii by ethanol precipitation and gel filtration. Phosphorylated polymer of Mw approximately 75,000 contained 90% carbohydrates, 3.9% phosphorus and 5.3% proteins. Results of chemical and spectroscopic studies showed a highly branched structure of GalXM with a 1,6-linked mannopyranosyl-galactopyranosyl backbone (approximately 44%) branched predominantly at C-2 and C-3 of mannosyl, and C-3 of galactosyl residues by side chains terminated mainly by xylosyl and mannosyl residues, and to a less extent by arabinosyl and glucosyl ones.
Capek
P
P
Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, SK-845 38 Bratislava, Slovakia. chemcape@savba.sk
Dudíková
J
J
Matulová
M
M
Kolarova
N
N
D002851:Chromatography, High Pressure Liquid; D003454:Cryptococcus / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation / Q000235:genetics*; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification*
D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C043689:galactoxylomannan