[Construction and expression of non-fusional expression vector of the multi-epitope gene of hepatitis B virus].
OBJECTIVE
To study the cloning, expression and antigen of therapeutic multi-epitope gene of hepatitis B virus.


METHODS
The multi-epitope gene of hepatitis B virus(BPT) was designed, synthesized and cloned into the prokaryotic expression vector pBAD/gIIIA. Then it was transformed into E.coli Top10 and multi-epitope protein of hepatitis B virus(B-BPT) was expressed under the induction of Arabinose. The immunogenicity of the protein was analyzed by Western blot detection.


RESULTS
The recombinant plasmid pBAD/BPT was constructed successfully and the protein of multi-epitope gene of hepatitis B virus was expressed in E.coli. Western blot detection showed the protein had ideal antigenicity.


CONCLUSIONS
The design of therapeutic multi-epitope gene of hepatitis B virus is proved to be correct. The expressed protein may be a good therapeutic vaccine.
Luo
Li-Min
LM
Medical Center of Liver Diseases, 458 Hospital, Guangzhou 510602, China.
Xia
Hu
H
Li
Zhuo-Liang
ZL
Yu
Zhou-Yao
ZY
Liu
Shu-Ren
SR
Li
Ming
M
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D017325:Hepatitis B Vaccines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology*
D000939:Epitopes; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines