Enterocytozoon bieneusi genotypes in dogs in Bogota, Colombia.
Enterocytozoon bieneusi was detected in dogs in Bogota, Colombia for the first time. Of 120 dogs, 15% were positive by polymerase chain reaction (PCR). Infected dogs ranged from 2 to 14 years of age, and more male than female dogs were infected with E. bieneusi (20% versus 5%). All PCR-positive specimens were sequenced and three genotypes were identified. A dog-specific genotype (PtEbIX) was identified in feces from 16 dogs. For two dogs, the nucleotide sequences obtained were genetically identical to the E. bieneusi Peru 5 and K genotypes previously reported as human pathogens. This is the first time that Peru 5 and K genotypes have been identified in dogs. This study confirms that dogs are infected with both human pathogenic and host-specific genotypes.
Santín
Mónica
M
Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA. monica-santin-duran@ars.usda.gov
Cortés Vecino
Jesús A
JA
Fayer
Ronald
R
D000818:Animals; D003105:Colombia / Q000453:epidemiology; D004271:DNA, Fungal / Q000737:chemistry; D004283:Dog Diseases / Q000453:epidemiology / Q000382:microbiology*; D004285:Dogs; D021862:Enterocytozoon / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005260:Female; D005838:Genotype; D008297:Male; D016881:Microsporidiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary
D004271:DNA, Fungal