title: Expression of transketolase-like 1 (TKTL1) and p-Akt  correlates with the progression of cervical neoplasia.
abstract: OBJECTIVE
  It is supposed that increased glycolysis is crucial for the energy supply during tumor progression. Unfortunately, the relevance of glycolysis in cervical neoplasia is unknown, but what is certain is the fact that cervical cancer shows a high expression of glucose membrane transporters, which are necessary for glucose uptake as an energy source. Transketolase-like enzyme 1 (TKTL1) and the oncogene p-Akt have been described to play an important role in glycolysis during tumorigenesis. Thus, we were interested in their expression in cervical tissue.
  
  
  METHODS
  We examined the expression of TKTL1 and p-Akt in 80 formalin-fixed, paraffin-embedded cervical specimens: 20 benign cervical tissues, 20 low-grade squamous intraepithelial lesions, 20 high-grade intraepithelial lesions, and 20 invasive squamous cell carcinomas (ISCC).
  
  
  RESULTS
  Immunhistochemical analyses revealed that the intensity of the expression of TKTL1 and p-Akt increases significantly with an increase in the histopathological grade of cervical tissues.
  
  
  CONCLUSIONS
  The results suggest that both TKTL1 and p-Akt play an important role in the progression of cervical neoplasia, which may be due to their impact on glycolysis.
authors.affiliation: Departments of Obstetrics and Gynecology, University of Wuerzburg, Wuerzburg, Germany. kohrenhagen@email.de <kohrenhagen@email.de>
authors.forename: Nico
authors.initials: N
authors.lastname: Kohrenhagen
authors.forename: Hans U
authors.initials: HU
authors.lastname: Voelker
authors.forename: Melanie
authors.initials: M
authors.lastname: Schmidt
authors.forename: Michaela
authors.initials: M
authors.lastname: Kapp
authors.forename: Matthias
authors.initials: M
authors.lastname: Krockenberger
authors.forename: Torsten
authors.initials: T
authors.lastname: Frambach
authors.forename: Johanees
authors.initials: J
authors.lastname: Dietl
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kammerer
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000473:pathology; D002584:Cervix Uteri / Q000737:chemistry; D005260:Female; D006019:Glycolysis; D006801:Humans; D007150:Immunohistochemistry; D016612:Paraffin Embedding; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D014174:Transketolase / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000737:chemistry / Q000473:pathology
chemical_list: C505431:TKTL1 protein, human; D014174:Transketolase; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt