title: Lipoic acid rescues DBA mice from early-onset age-related hearing impairment.
abstract: We fed DBA mice with alpha-lipoic acid (100 mg/kg body weight/day), beginning 2, 4, or 8 weeks after birth. Hearing thresholds were measured weekly. At 12 weeks after birth, control mice not fed with alpha-lipoic acid showed significant hearing decreases at all frequencies. In contrast, mice fed with alpha-lipoic acid beginning at 2 weeks after birth showed significantly better hearing at all frequencies. Mice fed with alpha-lipoic acid beginning at 4 and 8 weeks after birth also showed significantly better hearing than control mice after they were fed with alpha-lipoic acid. The stria vascularis of mice fed with alpha-lipoic acid showed reduced 8-oxoguanine residues in DNA and cytoplasm compared with that of control mice. Western blotting showed that the level of hypoxia-inducible factor-1alpha was lower in mice fed with alpha-lipoic acid than in control mice. From these results, we suggest that alpha-lipoic acid prevented early-onset hearing impairment in DBA mice.
authors.affiliation: Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Head and Neck Surgery, Konkuk University Hospital, Seoul, Korea.
authors.forename: Joong Ho
authors.initials: JH
authors.lastname: Ahn
authors.forename: Hun Hee
authors.initials: HH
authors.lastname: Kang
authors.forename: Tae Yong
authors.initials: TY
authors.lastname: Kim
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Shin
authors.forename: Jong Woo
authors.initials: JW
authors.lastname: Chung
mesh_terms: D000367:Age Factors; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000627:therapeutic use; D001309:Auditory Threshold / Q000187:drug effects / Q000502:physiology; D015153:Blotting, Western; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D006309:Hearing / Q000187:drug effects* / Q000502:physiology; D034381:Hearing Loss / Q000503:physiopathology / Q000517:prevention & control*; D006320:Hearing Tests / Q000379:methods; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051379:Mice; D008811:Mice, Inbred DBA; D013316:Stria Vascularis / Q000187:drug effects / Q000378:metabolism; D008063:Thioctic Acid / Q000378:metabolism / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C024829:8-hydroxyguanine; D006147:Guanine; D008063:Thioctic Acid