[Expression of p27 and TGF-beta in rat kidney with unilateral ureteral obstruction and the relationship between TGF-beta and p27].
OBJECTIVE
To explore the expression of p27 and the changes of TGF-beta in the renal tubule on the process of renal interstitial fibrosis caused by unilateral ureteral obstruction (UUO) in rats and to investigate the relationship between TGF-beta and p27 in renal interstitial fibrosis rats.


METHODS
Sixty rats were randomly divided into sham-operated group (SOR) and UUO group. Ten rats from each group were sacrificed on days 7, 14 and 21 after operation respectively. The renal pathological changes were dynamically observed by HE . The expression and dynamic changes of p27 were detected by immunohistochemistry. The levels of p27 mRNA and TGF-beta mRNA were detected by RT-PCR.


RESULTS
The expression of p27 in renal tubular epithelial cells and p27mRNA was strongly positive in SOR group. With the aggravation of interstitial fibrosis, the expression of p27 gradually decreased in UUO group while the expression of TGF-beta increased. There was a negative correlation between TGF-beta and p27.


CONCLUSIONS
P27 is involved in the fibrosis of renal mesenchyme in rats with UUO. The mechanism of TGF-beta accelerating fibrosis may relate to the reduction of p27 expression in UUO rats.
Sun
Jian
J
Department of Nephrology, Xiangya Hospital, Basic Medical College, Central South University, Changsha 410008, China. sunjian105@sina.com.cn
Tao
Li-Jian
LJ
Ning
Wang-Bin
WB
Jin
Ou
O
Liu
Ji-Shi
JS
D000818:Animals; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics*; D005260:Female; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014517:Ureteral Obstruction / Q000235:genetics*
D016212:Transforming Growth Factor beta; D050760:Cyclin-Dependent Kinase Inhibitor p27