Immunoregulatory and anti-tumor effects of polysaccharopeptide and Astragalus polysaccharides on tumor-bearing mice.
The aim of this study was to determine whether polysaccharopeptide (PSP) and Astragalus polysaccharides (APS) can be combined together as a new complex prescription (PSP + APS) for aiding adriamycin (AMD) chemotherapy. Ehrlich's ascites carcinoma (EAC) was used to establish a solid tumor model in Kunming mice. Immunocytochemical and immunohistochemical analysis were employed to detect the immunoregulatory and anti-tumor effects of EAC bearing mice after 30 days of administration with PSP and APS. PSP and PSP + APS could significantly increase the percentage of CD3(+) and CD4(+) T-lymphocytes, the ratio of CD4(+)/CD8(+), and the expression of IL-2/IL-2R in spleen and Bax in tumor tissue, but led to a diminution of Bcl-2 and CDK4 in tumor tissue compared with those of control group. In addition, PSP +APS could restore the immunological effects against AMD-induced immunosuppression, such as the subset of leukomonocyte, the expression of IL-2/IL-2R in the spleen, and the thymus index. These findings suggest that the immunomodulatory and anti-cancer effects of this new formula (PSP+APS) were better than those of PSP alone, and also could resist immunosuppression induced by AMD.
Li
Jin
J
Department of Immunology, Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing, China.
Bao
Yixi
Y
Lam
Waikei
W
Li
Wenwen
W
Lu
Fang
F
Zhu
Xuan
X
Liu
Jing
J
Wang
Hongping
H
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D027883:Astragalus Plant / Q000276:immunology*; D027885:Astragalus propinquus; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000276:immunology*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D005260:Female; D007155:Immunologic Factors / Q000008:administration & dosage / Q000627:therapeutic use*; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use; D011509:Proteoglycans / Q000008:administration & dosage / Q000627:therapeutic use*; D011897:Random Allocation
D000970:Antineoplastic Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D010936:Plant Extracts; D011509:Proteoglycans; C093216:polysaccharide peptide; C027492:Huang Qi