title: [Influence of electromagnetic field on chosen parameters of thrombocytes' oxygen metabolism--in vitro research].
abstract: Being a natural environmental factor, an electromagnetic field exists from the beginning of the life on Earth and it has an influence on maintenance of life processes. Natural electromagnetic fields affect day and year rhythms of plants, animals and humans. As a result of an electromagnetic field's activity, there occurs a disorder of blood platelets' function, which may, in consequence, lead to acute and chronic conditions dangerous to health and life.
  
  
  OBJECTIVE
  The aim of this work was to assess the influence, which a shape of an electromagnetic field of low frequency has on generating free radicals and enzymatic activity of superoxide dismutase in human blood platelets.
  
  
  METHODS
  Suspension of human blood platelets was subjected to activity of electromagnetic field of different shapes, frequency of 50 Hz and induction of 10 mT for 15 and 30 minutes. An electromagnetic field was generated with Helmholtz coils arranged on a bracket, inside of which test tubes with the blood platelets' suspension were put. Next, they were subjected to an activity of a specific electromagnetic field.
  
  
  RESULTS
  The measurement of free radicals generation indicated an increase, in comparison with the initial values, after 15 minutes as well as 30 minutes of exposition, regardless of the electromagnetic field's shape, whereas the enzymatic activity of superoxide dismutase decreased, in comparison with the initial values, after 15 minutes as well as 30 minutes of exposition, regardless of the applied electromagnetic field's shape.
  
  
  CONCLUSIONS
  Basing on obtained results, it may be stated that the level of generating free radicals as well as the level of enzymatic activity of superoxide dismutase in tested blood cells indicates significant dependence on an electromagnetic field's shape. The greatest changes have been observed during the activity of a rectangular and triangular pulse.
authors.affiliation: Uniwersytet Medyczny w Łodzi, Zakład Edukacji Zdrowotnej i Promocji Zdrowia. promzdr@achilles.wam.lodz.pl
authors.forename: Wojciech
authors.initials: W
authors.lastname: Jankowski
authors.forename: Gabriela
authors.initials: G
authors.lastname: Henrykowska
authors.forename: Janusz
authors.initials: J
authors.lastname: Smigielski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Pacholski
authors.forename: Maria
authors.initials: M
authors.lastname: Dziedziczak-Buczyńska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kałka
authors.forename: Andrzej
authors.initials: A
authors.lastname: Buczyński
mesh_terms: D001792:Blood Platelets / Q000378:metabolism* / Q000528:radiation effects*; D004574:Electromagnetic Fields; D005609:Free Radicals / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D010100:Oxygen / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D005609:Free Radicals; D013482:Superoxide Dismutase; D010100:Oxygen