title: Detection of HPV-DNA, p53 alterations, and methylation in penile squamous cell carcinoma in Japanese men.
abstract: Penile carcinoma is a rare disease, accordingly there are few studies on molecular changes, and these results also vary greatly. A total of 26 penile squamous cell carcinomas in Japanese men were studied with respect to HPV, p53 alterations, and methylation of gene promoter region. HPV-DNA was detected in three of 26 patients (11.5%). Overexpression of p53 was observed in 13 of 26 patients (50%), and p53 gene mutations were detected in four of 26 patients (15.4%). The frequency of methylation was as follows: DAPK, 26.9% (7/26); FHIT, 88.4% (23/26); MGMT, 19.2% (5/26); p14, 3.8% (1/26); p16, 23.1% (6/26); RAR-beta, 23.1% (6/26); RASSF1A, 11.5% (3/26); and RUNX3, 42.3% (11/26). As for correlation between HPV and p53 alterations, and methylation status, mutations of the p53 gene were detected only in HPV-negative patients, and methylation was more frequently found in HPV-negative than in HPV-positive patients. The present results suggest that the majority of penile squamous cell carcinomas in Japanese men are unrelated to HPV, and gene alterations accumulate more frequently in HPV-unrelated penile carcinomas.
authors.affiliation: Department of Pathology, Yamagata University School of Medicine, Tamagata, Japan. nyanagaw@med.id.yamagata-u.ac.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Yanagawa
authors.forename: Mitsumasa
authors.initials: M
authors.lastname: Osakabe
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hayashi
authors.forename: Gen
authors.initials: G
authors.lastname: Tamura
authors.forename: Teiichi
authors.initials: T
authors.lastname: Motoyama
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics / Q000378:metabolism; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000821:virology; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics / Q000378:metabolism; D019941:Cyclin-Dependent Kinase Inhibitor p16; D019175:DNA Methylation; D004279:DNA, Viral / Q000032:analysis*; D064550:Death-Associated Protein Kinases; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D008297:Male; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections; D010412:Penile Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D050677:Core Binding Factor Alpha 3 Subunit; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral; D064550:Death-Associated Protein Kinases; D009363:Neoplasm Proteins; D018168:Receptors, Retinoic Acid; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D000099170:Bis(5'-adenosyl)triphosphatase; C000614131:CDKN2A protein, human; C411762:RASSF1 protein, human; C493729:Runx3 protein, human; C082925:retinoic acid receptor beta