title: Establishment of a highly metastatic tongue squamous cell carcinoma cell line from New Zealand White rabbit.
abstract: OBJECTIVE
  Prior to this study, the widely used tongue squamous cell carcinoma cell lines could only initiate tumours in immunodeficient mice, which greatly delayed studies on immune function during carcinogenesis. This study established a new tongue squamous cell carcinoma cell line named 'RSCC-1', which can initiate tumours in both immunocompetent rabbits and immunodeficient nude mice and has high metastatic ability.
  
  
  METHODS
  Primary tongue cancer was induced by DMBA and local mechanical stimulation in New Zealand White rabbits. The induced cancer was serially transplanted into homogeneous rabbits to establish transplanted models. At the same time, cancer samples were collected, cultured and passaged in vitro. Finally, a cell line named 'RSCC-1' was established. Its growth behaviour, cell cycle distribution and tumourigenicity in rabbits and nude mice were investigated.
  
  
  RESULTS
  RSCC-1 cells were cultured continuously in vitro for 19 months (165 passages). They contain between 54 and 196 chromosomes, with a modal number of 75. Tumourigenicity rates were 100% in both homogeneous rabbits and nude mice, with 20% lung metastasis and 50% cervical lymph node metastasis in homogeneous rabbits.
  
  
  CONCLUSIONS
  RSCC-1 is a poorly differentiated, highly malignant rabbit tongue squamous cell carcinoma cell line. Its behaviour in the inoculated animal model closely resembles human tongue cancer, and could metastasize to local lymph nodes and remote organs.
authors.affiliation: Department of Head and Neck Surgery, Ear Nose and Throat Hospital, Fudan University, Shanghai 200031, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wulong
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xiaojian
authors.forename: Tian
authors.initials: T
authors.lastname: Jie
authors.forename: Guo
authors.initials: G
authors.lastname: Huilin
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000473:pathology / Q000556:secondary* / Q000648:ultrastructure; D002453:Cell Cycle; D002455:Cell Division; D045744:Cell Line, Tumor / Q000473:pathology* / Q000648:ultrastructure; D004195:Disease Models, Animal; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D011817:Rabbits; D014062:Tongue Neoplasms / Q000139:chemically induced / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D015127:9,10-Dimethyl-1,2-benzanthracene