EasyBeacons for the detection of methylation status of single CpG duplets.
This chapter describes two different fast protocols for optimizing EasyBeacons for the challenging detection of the methylation status of a single CpG duplet in bisulfitetreated DNA. EasyBeacons can be used in multiplex detections even if they do not have the same affinity for their respective targets giving very specific results for both realtime polymerase chain reaction (real-time PCR) detection and endpoint analysis using inexpensive master mixes. The technology described in this chapter is a very competitive alternative to other real-time PCR technologies.
Christensen
Ulf B
UB
PentaBase, Soendersoe, Denmark.
D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D015226:Dinucleoside Phosphates / Q000032:analysis*; D005456:Fluorescent Dyes; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013447:Sulfites / Q000494:pharmacology
D017931:DNA Primers; D015226:Dinucleoside Phosphates; D005456:Fluorescent Dyes; D013447:Sulfites; C015772:CpG dinucleotide; C042345:hydrogen sulfite