title: Generation of neuronal-like cells from umbilical cord blood-derived mesenchymal stem cells of a RFP-transgenic cloned cat.
abstract: Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are multipotent adult stem cells, which can differentiation into cells of connective tissue and neural lineages. This study investigated the potential for neuronal differentiation of red fluorescent protein (RFP)-transgenic cat UCB-derived MSCs. The cells were cultured in pre-induction medium for 24 hr and in neuronal-induction medium for 72 hr. Immunofluorescent staining showed that 6.85% of the total cells were beta III-tubulin-positive, 3.37% were neurofilament light (NF-L)-positive and 7.04% were neurofilament medium (NF-M)-positive. A beta III-tubulin band was detected by western blot analysis. Our results demonstrate that RFP-transgenic UCB-derived MSCs can be differentiated into neuronal cells in vitro. Thus, RFP-transgenic MSCs could provide alternative tracing material for stem cell transplantation.
authors.affiliation: Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University, Jinju, GyeongNam Province, South Korea.
authors.forename: Guang-Zhen
authors.initials: GZ
authors.lastname: Jin
authors.forename: Xi-Jun
authors.initials: XJ
authors.lastname: Yin
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Yu
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Cho
authors.forename: Eu-Gene
authors.initials: EG
authors.lastname: Choi
authors.forename: Young-S
authors.initials: YS
authors.lastname: Lee
authors.forename: Jin-Tae
authors.initials: JT
authors.lastname: Jeon
authors.forename: Sung-Tae
authors.initials: ST
authors.lastname: Yee
authors.forename: Il-Keun
authors.initials: IK
authors.lastname: Kong
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002415:Cats / Q000097:blood*; D002454:Cell Differentiation / Q000502:physiology; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry / Q000662:veterinary; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence / Q000662:veterinary; D009474:Neurons / Q000166:cytology*; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein