Effects of microalgae Chlorella species crude extracts on intestinal adaptation in experimental short bowel syndrome.
OBJECTIVE
To evaluate the effects of chlorella crude extract (CCE) on intestinal adaptation in rats subjected to short bowel syndrome (SBS).


METHODS
Wistar rats weighing 230-260 g were used in the study. After anesthesia a 75% small bowel resection was performed. Rats were randomized and divided into groups. Control group (n = 10): where 5% dextrose was given through a gastrostomy tube, Enteral nutrition (EN) group (n = 10): Isocaloric and isonitrogen EN (Alitraq, Abbott, USA), study group (n = 10): CCE was administrated through a gastrostomy tube. Rats were sacrificed on the fifteenth postoperative day and blood and tissue samples were taken. Histopathologic evaluation, intestinal mucosal protein and DNA levels, intestinal proliferation and apoptosis were determined in intestinal tissues, and total protein, albumin and citrulline levels in blood were studied.


RESULTS
In rats receiving CCE, villus lengthening, crypt depth, mucosal DNA and protein levels, intestinal proliferation, and serum citrulline, protein and albumin levels were found to be significantly higher than those in control group. Apoptosis in CCE treated rats was significantly reduced when compared to EN group rats.


CONCLUSIONS
CCE has beneficial effects on intestinal adaptation in experimental SBS.
Kerem
Mustafa
M
Department of General Surgery, Gazi University, Besevler 06510, Ankara, Turkey. mkerem@gazi.edu.tr
Salman
Bulent
B
Pasaoglu
Hatice
H
Bedirli
Abdulkadir
A
Alper
Murat
M
Katircioglu
Hikmet
H
Atici
Tahir
T
Percin
E Ferda
EF
Ofluoglu
Ebru
E
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002708:Chlorella; D002956:Citrulline / Q000097:blood; D004247:DNA / Q000378:metabolism; D004195:Disease Models, Animal; D007082:Ileum / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000378:metabolism; D012778:Short Bowel Syndrome / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
D010936:Plant Extracts; D012709:Serum Albumin; D002956:Citrulline; D004247:DNA