Using G-deleted vesicular stomatitis virus to probe the innate anti-viral response.
A method is described for the use of a G-deleted, conditionally replicating version of vesicular stomatitis virus (VSV) to measure alterations to the innate anti-viral state of cells in vitro. By co-transfecting a gene of interest with an expression vector for VSV-G one can directly measure the replication of the virus in the transfected cells as non-transfected cells will fail to produce infectious progeny due to the lack of glycoprotein in these non-transfected cells. This allows the investigator to focus exclusively on the anti-viral state induced or inhibited in the transfected cells allowing for screening and quantitative analysis of viral or cellular genes that may modify the anti-viral state.
Lai
Frances
F
Department of Medical Sciences, Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, 1200 Main Street West, MDCL 5023, Hamilton, Ontario, Canada L8N 3Z5.
Kazdhan
Natasha
N
Lichty
Brian D
BD
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D017353:Gene Deletion; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate; D007372:Interferons / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics*; D011506:Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology* / Q000472:pathogenicity*; D014759:Viral Envelope Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D011506:Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D007372:Interferons