title: Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA.
abstract: Spectrofluorometric titration, electrospray ionization time-of-flight mass spectrometric and UV melting methods were employed to study the binding of chelerythrine and sanguinarine to bulged DNA. The results showed that both alkaloids bind specifically to single pyrimidine (C, T) bulge sites. The ability of sanguinarine to bind to both regular and bulged hairpins was found to be stronger than that of chelerythrine, but the binding selectivity of chelerythrine toward single-base bulges was much larger than that of sanguinarine.
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Bai
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
authors.forename: Zhong-Zhen
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakatani
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Jiang
mesh_terms: D020029:Base Pairing; D053119:Benzophenanthridines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007546:Isoquinolines / Q000737:chemistry*; D015394:Molecular Structure; D011743:Pyrimidines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D017186:Titrimetry; D044366:Transition Temperature
chemical_list: D053119:Benzophenanthridines; D007546:Isoquinolines; D011743:Pyrimidines; D004247:DNA; C005705:sanguinarine; C016299:chelerythrine; C030986:pyrimidine