title: Sample preparation method for plasma membrane proteome analysis.
abstract: The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, 1, 5-ga Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
authors.forename: Seung-Ah
authors.initials: SA
authors.lastname: Park
authors.forename: Mi-Ryung
authors.initials: MR
authors.lastname: Kim
authors.forename: Pan-Kyeom
authors.initials: PK
authors.lastname: Kim
authors.forename: Hyung-Don
authors.initials: HD
authors.lastname: Cho
authors.forename: Gi-Yeon
authors.initials: GY
authors.lastname: Han
authors.forename: Chan-Wha
authors.initials: CW
authors.lastname: Kim
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome; D014461:Ultracentrifugation
chemical_list: D001798:Blood Proteins; D020543:Proteome