Spectroscopic labeling of A, S/T in the 1H-15N HSQC spectrum of uniformly (15N-13C) labeled proteins.
A new triple resonance two-dimensional experiment, termed (HC)NH, has been described to generate specific labels on the peaks of alanines and serines/threonines, separately, in the (1)H-(15)N HSQC spectrum of a protein. The performance of the pulse sequence has been demonstrated with a 151 residue protein. The method permits the investigation of local environments around those specific residues without actually having to obtain complete resonance assignments for the entire protein. With this one can envisage use of the technique for studying large protein systems from different points of view.
Chugh
Jeetender
J
Department of Chemical Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, India.
Hosur
Ramakrishna V
RV
D000409:Alanine / Q000737:chemistry*; D000605:Amino Alcohols / Q000737:chemistry*; D002072:Butylene Glycols / Q000737:chemistry*; D002247:Carbon Isotopes / Q000737:chemistry*; D009587:Nitrogen Isotopes / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D011522:Protons; D012694:Serine / Q000737:chemistry*; D013113:Spin Labels; D013194:Staining and Labeling
D000605:Amino Alcohols; D002072:Butylene Glycols; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011506:Proteins; D011522:Protons; D013113:Spin Labels; C532652:threoninol; D012694:Serine; D000409:Alanine