Localization of the ActIII actinorhodin polyketide ketoreductase to the cell wall.
Structurally diverse polyketides provide a rich reservoir of bioactive molecules. Actinorhodin, a model aromatic polyketide, is synthesized by minimal type II polyketide synthase and tailoring enzymes. The ActIII actinorhodin ketoreductase is a key tailoring enzyme in actinorhodin biosynthesis. With purified antibodies against actinorhodin polyketide synthase alpha subunit (KSalpha) and ketoreductase, we conducted systematic localization experiments of the two proteins in Streptomyces coelicolor subproteomes. The results support the membrane location of KSalpha and cell-wall location of ketoreductase. Considering previous evidence that some other tailoring enzymes of actinorhodin biosynthesis may be located outside the cytoplasm, a picture is emerging of an extensive role for extracellular biochemistry in the synthesis of type II polyketide antibiotic.
Xu
Xin-Ping
XP
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Wang
Zhi-Jun
ZJ
Fan
Ke-Qiang
KQ
Wang
Sheng-Lan
SL
Jia
Cui-Juan
CJ
Han
Hui
H
Ramalingam
Eswar
E
Yang
Ke-Qian
KQ
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000906:Antibodies / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D002477:Cells / Q000187:drug effects; D003902:Detergents / Q000378:metabolism; D015870:Gene Expression; D009113:Muramidase / Q000494:pharmacology; D021122:Protein Subunits / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000201:enzymology* / Q000235:genetics
D000906:Antibodies; D001426:Bacterial Proteins; D003902:Detergents; D021122:Protein Subunits; D000429:Alcohol Oxidoreductases; C062921:actIII protein, Streptomyces coelicolor; D009113:Muramidase