Antibiotic fusidic acid has strong interactions with negatively charged lipid membranes: an electrokinetic capillary chromatographic study.
Fusidic acid (FA), a narrow spectrum steroidal antibiotic, is useful for treatment of most skin, conjunctival, and corneal infections and also in infections caused by atypical microbes in the surface of the eye. Liposome electrokinetic capillary chromatography (LEKC) was used to study the interactions between FA and lipid membranes. Liposomes prepared by extrusion were composed of 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleyl-sn-glysero-3-phosphor-L-serine (POPS), cholesterol, FA, and sphingomyelin (SM) in various molar ratios. 26 different liposome dispersions were studied as dispersed (pseudostationary) phase in LEKC. The hydrophobicities of the liposomes were evaluated by calculating the retention factors of model neutral steroids. The retention factors were calculated using the EOF and the effective electrophoretic mobilities of the analytes and the liposomes. The latter were separately determined by capillary electrophoresis with a polyacrylamide (PAA)-coated capillary. FA-lipid membrane interactions were studied by determining the retention factor of FA. In addition, liposomes prepared from lipids extracted from Escherichia coli bacterium were studied and used as dispersed phase in LEKC for interaction studies between FA and lipid membranes.
Helle
Anne
A
Laboratory of Analytical Chemistry, Department of Chemistry, P.O. Box 55, 00014 University of Helsinki, Finland.
Mäkitalo
Johanna
J
Huhtanen
Jarkko
J
Holopainen
Juha M
JM
Wiedmer
Susanne K
SK
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary; D004586:Electrophoresis; D004926:Escherichia coli; D005672:Fusidic Acid / Q000737:chemistry / Q000378:metabolism*; D006531:HEPES; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D010710:Phosphates; D013256:Steroids / Q000737:chemistry / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002021:Buffers; D008051:Lipid Bilayers; D008081:Liposomes; D010710:Phosphates; D013256:Steroids; D005672:Fusidic Acid; D006531:HEPES