Intracellular uptake and photodynamic activity of water-soluble [60]- and [70]fullerenes incorporated in liposomes.
Water-soluble fullerenes have attracted attention as promising compounds that have been used to forge new paths in the field of photo-biochemistry. To prepare water-soluble fullerenes, we employed lipid-membrane-incorporated fullerenes (LMICx; x=60 or 70) by using the fullerene exchange method from a gamma-cyclodextrin (gamma-CD) cavity to vesicles. LMIC60 have low toxicity in the dark and engender cell death by photoirradiation (lambda>350 nm). Furthermore, the photodynamic activity of LMIC70 is 4.7-fold that of LMIC60 for the same photon flux (lambda>400 nm). One of the reasons for the higher phototoxicity of LMIC70 is the higher generation of singlet oxygen (1O2) in LMIC70 than in LMIC60. The difference between LMIC60 and LMIC70 is considered to be simply derived from the amount of light absorption in the 400-700 nm region that is suitable for photodynamic therapy (PDT). To the best of our knowledge, this is the first case in which biological activity of C70 and its derivatives toward HeLa cells has been assayed.
Doi
Yuki
Y
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan), Fax: (+81) 743-72-6091.|Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585 (Japan).
Ikeda
Atsushi
A
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan), Fax: (+81) 743-72-6091.
Akiyama
Motofusa
M
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan), Fax: (+81) 743-72-6091.
Nagano
Mai
M
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan), Fax: (+81) 743-72-6091.
Shigematsu
Tamami
T
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan), Fax: (+81) 743-72-6091.
Ogawa
Takuya
T
Graduate School of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan).
Takeya
Tatsuo
T
Graduate School of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan).
Nagasaki
Takeshi
T
Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585 (Japan).
D037741:Fullerenes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D015394:Molecular Structure; D010316:Particle Size; D010777:Photochemistry; D026082:Singlet Oxygen / Q000032:analysis / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry*
D037741:Fullerenes; D008081:Liposomes; D014867:Water; D026082:Singlet Oxygen