High-throughput crystallization-to-structure pipeline at RIKEN SPring-8 Center.
A high-throughput crystallization-to-structure pipeline for structural genomics was recently developed at the Advanced Protein Crystallography Research Group of the RIKEN SPring-8 Center in Japan. The structure determination pipeline includes three newly developed technologies for automating X-ray protein crystallography: the automated crystallization and observation robot system "TERA", the SPring-8 Precise Automatic Cryosample Exchanger "SPACE" for automated data collection, and the Package of Expert Researcher's Operation Network "PERON" for automated crystallographic computation from phasing to model checking. During the 5 years following April, 2002, this pipeline was used by seven researchers to determine 138 independent crystal structures (resulting from 437 purified proteins, 234 cryoloop-mountable crystals, and 175 diffraction data sets). The protocols used in the high-throughput pipeline are described in this paper.
Sugahara
Michihiro
M
Advanced Protein Crystallography Research Group, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.
Asada
Yukuhiko
Y
Shimizu
Katsumi
K
Yamamoto
Hitoshi
H
Lokanath
Neratur K
NK
Mizutani
Hisashi
H
Bagautdinov
Bagautdin
B
Matsuura
Yoshinori
Y
Taketa
Midori
M
Kageyama
Yuichi
Y
Ono
Naoko
N
Morikawa
Yuko
Y
Tanaka
Yukiko
Y
Shimada
Hiroki
H
Nakamoto
Takanobu
T
Sugahara
Mitsuaki
M
Yamamoto
Masaki
M
Kunishima
Naoki
N
D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D001330:Electronic Data Processing; D007564:Japan; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012371:Robotics / Q000379:methods; D020539:Sequence Analysis, Protein; D012984:Software
D011506:Proteins