Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA.
The diversity of biomolecules present in plasmid DNA (pDNA)-containing extracts and the structural and chemical similarities between pDNA and impurities are some of the main challenges of improving or establishing novel purification procedures. In view of the unequalled specificity of affinity purification, this technique has recently begun to be applied in downstream processing of plasmids. This paper discusses the progress and importance of affinity chromatography (AC) for the purification of pDNA-based therapeutic products. Several affinity approaches have already been successfully developed for a variety of applications, and we will focus here on highlighting their possible contributions to the pDNA purification challenge. Diverse affinity applications and their advantages and disadvantages are discussed, as well as the most significant results and improvements in the challenging task of purifying plasmids.
Sousa
Fani
F
CICS-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, 6201-001 Covilhã, Portugal.
Prazeres
Duarte M F
DM
Queiroz
João A
JA
D000818:Animals; D001688:Biological Products / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000639:trends; D004247:DNA / Q000302:isolation & purification; D006801:Humans; D010957:Plasmids / Q000032:analysis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D001688:Biological Products; D004247:DNA