Enantioseparations with cellulose tris(3-chloro-4-methylphenylcarbamate) in nano-liquid chromatography and capillary electrochromatography.
Cellulose tris(3-chloro-4-methylphenylcarbamate) was coated onto native and aminopropylsilanized silica in order to prepare chiral stationary phases (CSPs) for enantioseparations using nano-liquid chromatography (nano-LC) and capillary electrochromatography (CEC). The effect of the chiral selector loading onto silica, mobile phase composition and pH, as well as separation variables on separation of enantiomers was studied. It was found that CSPs based on cellulose tris(3-chloro-4-methylphenylcarbamate) can be used for preparation of very stable capillary columns useful for enantioseparations in nano-LC and CEC in combination with polar organic mobile phases.
Fanali
Salvatore
S
Institute of Chemical Methodologies, Consiglio Nazionale delle Ricerche, Area della Ricerca di Roma I, Via Salaria Km 29,300, 00016 Monterotondo Scalo, Roma, Italy. salvatore.fanali@imc.cnr.it
D'Orazio
Giovanni
G
Lomsadze
Ketevan
K
Chankvetadze
Bezhan
B
D053801:Capillary Electrochromatography / Q000379:methods*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D053841:Electroosmosis; D036103:Nanotechnology / Q000379:methods; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D048448:Phenylcarbamates / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism
D004364:Pharmaceutical Preparations; D048448:Phenylcarbamates; C021611:aminopropyl silica gel; C538979:cellulose tris(3-chloro-4-methylphenylcarbamate); D012822:Silicon Dioxide; D002482:Cellulose