Inter- and intra-assay reproducibility of microplate Alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis.
The intersample and intrasample variability of the results obtained with the microplate Alamar blue assay for the indirect drug susceptibility testing of Mycobacterium tuberculosis was investigated. Between 1.2 and 8.5% of paired MICs differed by more than one twofold dilution, resulting in discordant susceptible-resistant designations at frequencies between 0.6% (rifampin) and 18.9% (ethambutol).
Leonard
Brian
B
Laboratorio de Investigación de Enfermedades Infecciosas, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado No. 430, San Martín de Porras, Lima 31, Perú.
Coronel
Jorge
J
Siedner
Mark
M
Grandjean
Louis
L
Caviedes
Luz
L
Navarro
Pilar
P
Gilman
Robert H
RH
Moore
David A J
DA
D000995:Antitubercular Agents / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D010078:Oxazines / Q000378:metabolism; D015203:Reproducibility of Results; D014966:Xanthenes / Q000378:metabolism
D000995:Antitubercular Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin