title: Application of high-performance liquid chromatography with amperometric and coulometric detection to the analysis of SZ1677, a new neuromuscular blocking agent, and its two derivatives.
abstract: A HPLC method with amperometric and coulometric detection for the determination of SZ1677 and its two derivatives SZ1676 and SZ1823 has been developed. This active substance is under development (clinical trial) and there are no analytical methods published for the determination of SZ1677 thus far. The limit of quantitation for SZ1677 was 25 and 100 ng ml(-1) by the coulometric and amperometric detection, respectively. The elaborate method for the simultaneous analysis of SZ1677 and its two derivatives proved to be fast, precise, accurate and sensitive.
authors.affiliation: Department of Pharmaceutical Chemistry, National Medicines Institute, 30/34 Chełmska Street, Warsaw, Poland. ablazewicz@il.waw.pl
authors.forename: Agata
authors.initials: A
authors.lastname: Błazewicz
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Fijałek
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Warowna-Grześkiewicz
authors.forename: Justyna
authors.initials: J
authors.lastname: Banasiuk
mesh_terms: D000731:Androstanes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D009466:Neuromuscular Blocking Agents / Q000032:analysis*; D012015:Reference Standards
chemical_list: C458501:1-(3-hydroxy-17-acetyloxy)-2-(1.4-dioxa-8-azaspiro-(4,5)-dec-8-yl)-(5alpha-androstane-16-yl)-1-(2-propenyl) pyrrolidine; D000731:Androstanes; D009466:Neuromuscular Blocking Agents