Rolling circle amplification: applications in nanotechnology and biodetection with functional nucleic acids.
Rolling circle amplification (RCA) is an isothermal, enzymatic process mediated by certain DNA polymerases in which long single-stranded (ss) DNA molecules are synthesized on a short circular ssDNA template by using a single DNA primer. A method traditionally used for ultrasensitive DNA detection in areas of genomics and diagnostics, RCA has been used more recently to generate large-scale DNA templates for the creation of periodic nanoassemblies. Various RCA strategies have also been developed for the production of repetitive sequences of DNA aptamers and DNAzymes as detection platforms for small molecules and proteins. In this way, RCA is rapidly becoming a highly versatile DNA amplification tool with wide-ranging applications in genomics, proteomics, diagnosis, biosensing, drug discovery, and nanotechnology.
Zhao
Weian
W
Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton, ON, L8P 4M1, Canada.
Ali
M Monsur
MM
Brook
Michael A
MA
Li
Yingfu
Y
D004247:DNA / Q000032:analysis* / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques
D004247:DNA; D004259:DNA-Directed DNA Polymerase