title: Bovine intelectins: cDNA sequencing and expression in the bovine intestine.
abstract: Intelectins (Itlns) are lectins with potential roles in innate immunity, capable of binding bacteria via galactofuranose residues. Itlns also function as intestinal receptors for the antimicrobial glycoprotein lactoferrin (Lf). Since Lf binds strongly to enterohemorrhagic Escherichia coli O157:H7 (EHEC), we aimed to determine the expression of Lf receptor in terminal rectum, the site of predilection of EHEC in cattle. We sequenced two bovine intelectins (Itln1 and Itln2) and showed that both were expressed in abomasum and rectum, but expression appeared minimal in the jejunum. There was significantly higher expression of Itln2 in terminal rather than proximal rectum. Lactoferrin was expressed in all samples examined. Thus, we have demonstrated two novel bovine Itlns and shown that they are expressed along with Lf in the gastrointestinal tract, where they may interact with microbial pathogens.
authors.affiliation: Division of Veterinary Clinical Sciences, University of Edinburgh, Royal (Dick) School of Veterinary Studies, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Stacey C
authors.initials: SC
authors.lastname: Blease
authors.forename: Anne T
authors.initials: AT
authors.lastname: French
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Knight
authors.forename: David L
authors.initials: DL
authors.lastname: Gally
authors.forename: Alan D
authors.initials: AD
authors.lastname: Pemberton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D018076:DNA, Complementary / Q000235:genetics; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D007410:Intestinal Diseases / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D011956:Receptors, Cell Surface; C038340:lactoferrin receptors; D012313:RNA