[Inhibitory effects of immunotargeting of Chinese cobra cytotoxin and iodine-131 against nasopharyngeal carcinoma cells in vitro].
OBJECTIVE
To explore a new specific therapy of nasopharyngeal carcinoma (NPC) using an anti-nasopharyngeal carcinoma (NPC) monoclonal antibody BAC5 conjugate with Chinese cobra (CT) and iodine-131(131I).


METHODS
BAC5 was labeled with 131I by chloramine-T method, CT was labeled with 125I using iodogen method, and BAC5 and 125I-CT were conjugated by SPDP method. The inhibitory effect of the conjugate on cultured human NPC CNE2 cells was observed using MTT assay.


RESULTS
The IC50 of 125I-CT-BAC5 conjugate was 9.17x10(-8) mol/L, and that of 131I-BAC5 was 5.83x10(8) Bq/L, and their combined administration showed obvious inhibitory effect on the NPC cells.


CONCLUSIONS
Both 125I-CT-BAC5 and 131I-BAC5 have obvious inhibition effects against NPC cells, but their combined use shows stronger effects.
Zhao
Yong-sheng
YS
Department of Nuclear Medicine, Second People's Hospital of Shenzhen/The Shenzhen Affiliated Hospital of Southern Medical University, Shenzhen 518035, China. szsait@163.com
Yang
Hui-ling
HL
Liu
Chang-zheng
CZ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003603:Cytotoxins / Q000494:pharmacology; D004546:Elapid Venoms / Q000494:pharmacology*; D006801:Humans; D018796:Immunoconjugates / Q000494:pharmacology*; D007457:Iodine Radioisotopes / Q000494:pharmacology*; D009303:Nasopharyngeal Neoplasms / Q000473:pathology
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D003603:Cytotoxins; D004546:Elapid Venoms; D018796:Immunoconjugates; D007457:Iodine Radioisotopes