title: Diffuse intralobular liver fibrosis in dogs naturally infected with Leishmania (Leishmania) chagasi.
abstract: The aim of this study was to evaluate the diffuse intralobular fibrosis in dogs naturally infected with Leishmania (Leishmania) chagasi. One hundred five infected animals with positive serologic tests for Leishmania were divided into two clinical groups: 69 symptomatic animals and 36 asymptomatic. Special staining with Gomori, Heidenhain, Silver, and Picrosirius Red was applied to characterize fibrilopoesis. The tissue parasite load was measured by immunohistochemistry and associated histomorphometric analyses. Intralobular fibrosis was observed in all dogs, and more collagen deposition was confirmed in the infected animals than in the controls by these histomorphometric studies. There were significant differences among the distinct clinical groups. In fact, symptomatic dogs showed an increased collagen deposition in the liver compared with asymptomatic ones. A peculiar diffuse intralobular fibrosis, where the collagen fibers encircled small groups of hepatocyte(s), was observed in two cases (1.9%).
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil. ferdinanmelo@yahoo.com.br
authors.forename: Ferdinan
authors.initials: F
authors.lastname: Melo
authors.forename: Marina
authors.initials: M
authors.lastname: Amaral
authors.forename: Patricia
authors.initials: P
authors.lastname: Oliveira
authors.forename: Wanderson
authors.initials: W
authors.lastname: Lima
authors.forename: Marina
authors.initials: M
authors.lastname: Andrade
authors.forename: Marilene
authors.initials: M
authors.lastname: Michalick
authors.forename: Pedro
authors.initials: P
authors.lastname: Raso
authors.forename: Washington
authors.initials: W
authors.lastname: Tafuri
authors.forename: Wagner
authors.initials: W
authors.lastname: Tafuri
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D004283:Dog Diseases / Q000469:parasitology / Q000473:pathology*; D004285:Dogs; D018314:Leishmania infantum / Q000302:isolation & purification / Q000472:pathogenicity*; D007898:Leishmaniasis, Visceral / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008103:Liver Cirrhosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D013194:Staining and Labeling
chemical_list: D003094:Collagen