title: Comparisons of complete RNA-2 sequences, pathological and serological features among three Japanese isolates of Arabis mosaic virus.
abstract: Arabis mosaic virus lily and narcissus isolates (ArMV-Li and ArMV-Na) induced severe necrotic spots on Chenopodium quinoa, whereas ArMV butterbur isolate (ArMV-Bu) caused symptomless infection in the plant. The accumulation level of ArMV-Bu in upper non-inoculated leaves of C. quinoa was comparable to that of ArMV-Li or -Na. The agar gel double-diffusion test using an antiserum against ArMV-Li showed ArMV-Li was closely related to ArMV-Na, but not to ArMV-Bu. The RNAs-2 of ArMV-Li, -Na, and -Bu consist of 3707, 3709, and 3789 nucleotides, and they contain one open reading frame encoding a putative polyprotein of 1083, 1084, and 1122 amino acids, respectively. The overall identity of RNA-2 of ArMV-Li displayed more than 90% with ArMV-Na, but less than 70% with ArMV-Bu. A phylogenetic analysis of 2A sequences from ArMV isolates revealed ArMV-Bu was not categorized in any cluster. ArMV-Bu is a unique isolate from the point of view of pathological and serological features, and nucleotide sequence.
authors.affiliation: Laboratory of Plant Pathology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 2528510, Japan. imura.yoshiyuki@nihon-u.ac.jp
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Imura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Oka
authors.forename: Kosuke
authors.initials: K
authors.lastname: Kimata
authors.forename: Morimasa
authors.initials: M
authors.lastname: Nasu
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Nakahama
authors.forename: Takanori
authors.initials: T
authors.lastname: Maeda
mesh_terms: D027465:Chenopodium quinoa / Q000821:virology*; D007564:Japan; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D012367:RNA, Viral