title: Comparison of hematologic values in blood samples with lithium heparin or dipotassium ethylenediaminetetraacetic acid anticoagulants in Hispaniolan Amazon parrots (Amazona ventralis).
abstract: Blood samples were collected from 20 Hispaniolan Amazon parrots (Amazona ventralis) and were divided into tubes that contained dipotassium ethylenediaminetetraacetic acid (K2EDTA) and lithium heparin. Complete blood cell counts were determined in each sample within 2 hours of collection. The level of agreement in results was moderate for plasma protein, packed cell volume (PCV), and leukocyte, monocyte, and lymphocyte counts between the anticoagulants. Plasma protein and PCV values were significantly lower in samples with lithium heparin than in those with K2EDTA, whereas lymphocyte numbers were significantly higher in lithium heparin samples than in K2EDTA samples. The level of agreement was good for the other cell types (heterophils, eosinophils, and basophils) when comparing the different anticoagulants. The poor level of agreement between anticoagulants with the increase in thrombocyte clumping in lithium heparin samples indicates that the use of lithium heparin as anticoagulant may affect thrombocyte count. No negative effects on morphology and staining of blood cells were apparent in smears from heparin samples compared with K2EDTA samples. Within the different values compared, the limits of agreement are small enough to be confident that lithium heparin can be used for routine CBC counts in a clinical setting. The use of the same anticoagulant should be recommended to follow trends within the same patient, especially when considering plasma protein concentration, PCV, and lymphocyte count.
authors.affiliation: Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803-8410, USA.
authors.forename: David Sanchez-Migallon
authors.initials: DS
authors.lastname: Guzman
authors.forename: Mark A
authors.initials: MA
authors.lastname: Mitchell
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Gaunt
authors.forename: Hugues
authors.initials: H
authors.lastname: Beaufrère
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Tully
mesh_terms: D046708:Amazona / Q000097:blood*; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001800:Blood Specimen Collection / Q000662:veterinary*; D004492:Edetic Acid / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000662:veterinary; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D006493:Heparin; D004492:Edetic Acid