Femtosecond laser-assisted microinjection into living neurons.
A Ti:sapphire femtosecond laser micro-manipulation system was used to perforate vital cells. Propidium iodide was successfully transferred into both astrocytes and PC12 cells via the channel of a femtosecond laser, which induced transiently recoverable opening of the cell membrane. The transfection efficiency reached almost 100%. This method also had the advantage of contact-free, non-disruptive and stable transfection.
Lei
Ming
M
State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
Xu
Hanpeng
H
Yang
Hao
H
Yao
Baoli
B
D000537:Aluminum Oxide; D000818:Animals; D007834:Lasers; D008845:Microinjections / Q000295:instrumentation* / Q000379:methods*; D018613:Microscopy, Confocal; D009474:Neurons / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D013995:Time; D014025:Titanium
D014025:Titanium; D000537:Aluminum Oxide