IL-24 expression at maternal-fetal interface and its roles in trophoblast invasion.
In this study, the expression of IL-24 at maternal-fetal interface and the roles in extravillous trophoblast (the TEV-1 cell line) invasion were examined. Immunohistochemistry was used to detect the expression of IL-24 in villi and decidual tissue. The proliferation of TEV-1 cells under the effect of IL-24 was measured by MTT assay. The invasiveness of TEV-1 cells under the effect of recombinant IL-24 (rhIL-24) was examined by transwell system. Immunohistochemical detection showed that IL-24 was expressed in the villi and decidual tissue, and distributed in villous column, trophoblasts, stroma and blood vessels. The proliferation of TEV-1 cells was not inhibited by rhIL-24 of various concentrations. The examination of invasion in vitro showed that rhIL-24 could inhibit the invasion of TEV-1 cells in a concentration-dependent manner. The results suggested IL-24 could inhibit the invasion of TEV-1 cells. Therefore, IL-24 produced by maternal-fetal interface in human first trimester pregnancy may influence the invasion of trophoblasts and is involved in normal pregnancy.
Cheng
Hong
H
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ch5166@163.com
Zou
Li
L
D002465:Cell Movement; D049109:Cell Proliferation; D002824:Chorionic Villi / Q000378:metabolism*; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D014327:Trophoblasts / Q000166:cytology*; D000099180:Interleukin-24
D007378:Interleukins; D011994:Recombinant Proteins; D000099180:Interleukin-24