title: High-throughput crystallization-to-structure pipeline at RIKEN SPring-8 Center.
abstract: A high-throughput crystallization-to-structure pipeline for structural genomics was recently developed at the Advanced Protein Crystallography Research Group of the RIKEN SPring-8 Center in Japan. The structure determination pipeline includes three newly developed technologies for automating X-ray protein crystallography: the automated crystallization and observation robot system "TERA", the SPring-8 Precise Automatic Cryosample Exchanger "SPACE" for automated data collection, and the Package of Expert Researcher's Operation Network "PERON" for automated crystallographic computation from phasing to model checking. During the 5 years following April, 2002, this pipeline was used by seven researchers to determine 138 independent crystal structures (resulting from 437 purified proteins, 234 cryoloop-mountable crystals, and 175 diffraction data sets). The protocols used in the high-throughput pipeline are described in this paper.
authors.affiliation: Advanced Protein Crystallography Research Group, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.
authors.forename: Michihiro
authors.initials: M
authors.lastname: Sugahara
authors.forename: Yukuhiko
authors.initials: Y
authors.lastname: Asada
authors.forename: Katsumi
authors.initials: K
authors.lastname: Shimizu
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Neratur K
authors.initials: NK
authors.lastname: Lokanath
authors.forename: Hisashi
authors.initials: H
authors.lastname: Mizutani
authors.forename: Bagautdin
authors.initials: B
authors.lastname: Bagautdinov
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Matsuura
authors.forename: Midori
authors.initials: M
authors.lastname: Taketa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kageyama
authors.forename: Naoko
authors.initials: N
authors.lastname: Ono
authors.forename: Yuko
authors.initials: Y
authors.lastname: Morikawa
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Hiroki
authors.initials: H
authors.lastname: Shimada
authors.forename: Takanobu
authors.initials: T
authors.lastname: Nakamoto
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Sugahara
authors.forename: Masaki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Naoki
authors.initials: N
authors.lastname: Kunishima
mesh_terms: D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D001330:Electronic Data Processing; D007564:Japan; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012371:Robotics / Q000379:methods; D020539:Sequence Analysis, Protein; D012984:Software
chemical_list: D011506:Proteins