title: Differences in transcriptional activity of cutaneous human papillomaviruses.
abstract: The interaction between UV-B irradiation and cutaneous human papillomaviruses (HPV) has been suggested to be of relevance for the development of non-melanoma skin cancers. We investigated the activity within the upstream regulatory region (URR) of the HPV types 8, 38, 92, 93 and 96, as well as their responsiveness to UV-B irradiation and cellular differentiation. Promoter activities were higher in HaCaT than in SiHa cells, corresponding to the HPV tissue tropism. Transcriptional start sites were mapped at P(92) (HPV-38), P(45) (HPV-92), P(7439) (HPV-93) and P(256) (HPV-96). Transcription from HPV-8, 93 and 96 URR was up-regulated by cellular differentiation, linking the activity of these HPVs to the cellular state. UV-B irradiation activated HPV-8 but inhibited HPV-38 and HPV-93 whereas HPV-92 and 96 were not affected. As there are variable UV-B responses among the HPV types, further studies of interactions between UV-B and HPV need to consider the HPV type.
authors.affiliation: Department of Medical Microbiology, Lund University, Malmö University Hospital, Malmö, Sweden.
authors.forename: Natasa
authors.initials: N
authors.lastname: Vasiljević
authors.forename: Lone
authors.initials: L
authors.lastname: Nielsen
authors.forename: Geoff
authors.initials: G
authors.lastname: Doherty
authors.forename: Joakim
authors.initials: J
authors.lastname: Dillner
authors.forename: Ola
authors.initials: O
authors.lastname: Forslund
authors.forename: Bodil
authors.initials: B
authors.lastname: Norrild
mesh_terms: D052685:Alphapapillomavirus / Q000235:genetics* / Q000528:radiation effects; D001483:Base Sequence; D045744:Cell Line, Tumor; D015967:Gene Expression Regulation, Viral; D006801:Humans; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000821:virology*; D011401:Promoter Regions, Genetic; D012867:Skin / Q000821:virology*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D014466:Ultraviolet Rays