Expression of laminin beta1 in hippocampi of patients with intractable epilepsy.
We investigated laminin beta1 expression in the hippocampi of patients with intractable epilepsy and explored the role of laminin beta1 in the pathogenesis of this condition. Fluorescence quantitative PCR, immunofluorescence, immunohistochemistry and Western blotting were used to measure laminin beta1 expression in surgically removed hippocampi of patients with intractable epilepsy, and the results were compared with control hippocampi. Fluorescence quantitative PCR showed increased expression of laminin beta1 mRNA in patient hippocampi compared with control tissues. Immunohistochemical staining demonstrated that laminin beta1 protein expression was significantly increased in patient hippocampi, and immunofluorescence microscopy showed accumulation of laminin beta1 in the cell membrane and cytoplasm of patient hippocampi. These findings were confirmed by Western blotting of protein preparations from patient hippocampi. Elevated expression of laminin beta1 mRNA and protein in the hippocampus suggests that laminin beta1 may play a role in the development of epileptic seizures in patients with intractable epilepsy.
Wu
Yuan
Y
Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, 400016 China.
Wang
Xue-feng
XF
Mo
Xue-an
XA
Sun
Hong-bin
HB
Li
Jin-mei
JM
Zeng
Yan
Y
Lin
Tao
T
Yuan
Jie
J
Xi
Zhi-qin
ZQ
Zhu
Xi
X
Zheng
Jin-ou
JO
D000293:Adolescent; D000328:Adult; D004827:Epilepsy / Q000473:pathology*; D015870:Gene Expression / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism
C478221:LAMB1 protein, human; D007797:Laminin; D012333:RNA, Messenger