title: Safety, immunogenicity, and efficacy of the ML29 reassortant vaccine for Lassa fever in small non-human primates.
abstract: A single injection of ML29 reassortant vaccine for Lassa fever induces low, transient viremia, and low or moderate levels of ML29 replication in tissues of common marmosets depending on the dose of the vaccination. The vaccination elicits specific immune responses and completely protects marmosets against fatal disease by induction of sterilizing cell-mediated immunity. DNA array analysis of human peripheral blood mononuclear cells from healthy donors exposed to ML29 revealed that gene expression patterns in ML29-exposed PBMC and control, media-exposed PBMC, clustered together confirming safety profile of the ML29 in non-human primates. The ML29 reassortant is a promising vaccine candidate for Lassa fever.
authors.affiliation: Institute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, United States. ilukashevich@ihv.umaryland.edu
authors.forename: Igor S
authors.initials: IS
authors.lastname: Lukashevich
authors.forename: Ricardo
authors.initials: R
authors.lastname: Carrion
authors.forename: Maria S
authors.initials: MS
authors.lastname: Salvato
authors.forename: Keith
authors.initials: K
authors.lastname: Mansfield
authors.forename: Kathleen
authors.initials: K
authors.lastname: Brasky
authors.forename: Juan
authors.initials: J
authors.lastname: Zapata
authors.forename: Cristiana
authors.initials: C
authors.lastname: Cairo
authors.forename: Marco
authors.initials: M
authors.lastname: Goicochea
authors.forename: Gia E
authors.initials: GE
authors.lastname: Hoosien
authors.forename: Anysha
authors.initials: A
authors.lastname: Ticer
authors.forename: Joseph
authors.initials: J
authors.lastname: Bryant
authors.forename: Harry
authors.initials: H
authors.lastname: Davis
authors.forename: Rasha
authors.initials: R
authors.lastname: Hammamieh
authors.forename: Maria
authors.initials: M
authors.lastname: Mayda
authors.forename: Marti
authors.initials: M
authors.lastname: Jett
authors.forename: Jean
authors.initials: J
authors.lastname: Patterson
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000276:immunology; D002144:Callithrix / Q000276:immunology*; D002522:Chlorocebus aethiops; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D007835:Lassa Fever / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D007836:Lassa virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007963:Leukocytes, Mononuclear / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008297:Male; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology*; D012449:Safety; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology; D014709:Vero Cells; D014766:Viremia / Q000276:immunology
chemical_list: D017252:CD3 Complex; D018950:Lipopolysaccharide Receptors