[Mechanism of the effect of Tongluo Recipe against glomerular sclerosis in rats].
OBJECTIVE
To investigate the effects of Tongluo Recipe on the expression of collagen IV (Col IV), fibronectin (FN), laminin (LN), transforming growth factor-beta1 (TGF-beta1) in rat renal tissues and explore the mechanism underlying these effects in rats with glomerular sclerosis.


METHODS
The pathological changes in the renal tissues of rats with glomerular sclerosis were observed microscopically, and the expressions of Col IV, FN, LN, and TGF-beta1 were detected using immunohistochemical staining and image analysis system.


RESULTS
Tongluo Recipe significantly decreased the expressions of Col IV, FN, LN and TGF-beta1 in the renal tissue of rats with glomerular sclerosis (P<0.05 or P<0.01) and obviously alleviated the renal pathologies (P<0.01).


CONCLUSIONS
The therapeutic effects of Tongluo Recipe are probably mediated by lowered expressions of Col IV, FN, LN and TGF-beta1.
Wu
Xi-li
XL
Department of Integrated Traditional Chinese and Western Medicine, Shaanxi Provincial Key TCM, Second Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. wuxili1984@163.com
Sun
Wan-sen
WS
Zhang
Wang-gang
WG
Wang
Zhu
Z
D000818:Animals; D024141:Collagen Type IV / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005353:Fibronectins / Q000096:biosynthesis; D005923:Glomerulosclerosis, Focal Segmental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis; D016896:Treatment Outcome
D024141:Collagen Type IV; D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D053773:Transforming Growth Factor beta1; C527174:tongluo