title: Expression of TLR4 and CD14 in the central nervous system (CNS) in a MPTP mouse model of Parkinson's-like disease.
abstract: Systemic infections are often associated with neurodegenerative processes in many diseases of the central nervous system (CNS), including Parkinson's disease. Toll-like receptor (TLR)4 and CD14 act as receptors for lipopolysaccharide (LPS) released by gram-negative bacteria. In this contest, CD14 functions as the main LPS ligand and TLR4 transmits the LPS signal into the cell. In this paper, we investigated the expression of TLR4 and CD14, in different anatomical areas of the CNS, in an experimental model of Parkinson's-like disease, represented by MPTP-treated mouse. In particular, we analyzed the gene transcripts and proteins expression of CD14 and TLR4, in the substantia nigra and caudate-putamen nuclei of these animals. Results demonstrated an augmented expression of both CD14 and TLR4 in the substantia nigra of mice treated with MPTP in comparison to untreated animals, suggesting that the endotoxin receptors are over expressed in different manner in specific areas of the CNS during Parkinson's-like disease.
authors.affiliation: Department of Human Anatomy and Histology, University of Bari, Bari, Italy. ma.panaro@anatomia.uniba.it
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Panaro
authors.forename: Dario Domenico
authors.initials: DD
authors.lastname: Lofrumento
authors.forename: Concetta
authors.initials: C
authors.lastname: Saponaro
authors.forename: Francesco
authors.initials: F
authors.lastname: De Nuccio
authors.forename: Antonia
authors.initials: A
authors.lastname: Cianciulli
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Mitolo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Nicolardi
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000818:Animals; D002490:Central Nervous System / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D020734:Parkinsonian Disorders / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018950:Lipopolysaccharide Receptors; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine