title: Effects of torsion, detorsion and melatonin on testicular malondialdehyde level.
abstract: BACKGROUND
  Unilateral testicular torsion is a cause of bilateral testicular damage, which has ischaemic and reperfusion components. The damage may involve lipid peroxidation leading to production of lipid peroxides in the testes, including malondialdehyde (MDA).
  
  
  OBJECTIVE
  To investigate the MDA variations in the ipsilateral and contralateral testes following ischaemia-reperfusion and the effect of melatonin.
  
  
  METHODS
  Mature adult male Sprague-Dawley rats were divided into 13 groups of 10 each. One control group underwent sham operation. Three groups were subjected to right sided testicular torsion by twisting the testes 720 degrees counterclockwise for one, three and five hours; three groups were subjected to de-torsion following torsion lasting one, three and five hours; three groups were treated with intra-peritoneal melatonin (1 mg/kg) before torsion lasting one, three and five hours, and three groups were treated with intra-peritoneal melatonin before de-torsion following torsion lasting one, three and five hours. At the end of the experiment all animals were sacrificed by decapitation and testes were collected for MDA level estimation.
  
  
  RESULTS
  The MDA level was significantly higher in ipsilateral torted testis than the control testis in all groups (P < 0.05), with the levels increasing with the duration of torsion. Detorsion significantly increased the MDA level only if the initial torsion was for less than three hours. Melatonin did not significantly affect the MDA level in the ipsilateral testis if administered before torsion, but significantly reduced the level if administered before detorsion.
  
  
  CONCLUSIONS
  Malondialdehyde levels are altered in the both testes following unilateral testicular torsion-detorsion injuries. The reperfusion component of the injury is significant and may be reduced by melatonin.
authors.affiliation: Department of Anatomy, College of Medicine, University of Lagos.
authors.forename: F I O
authors.initials: FI
authors.lastname: Duru
authors.forename: C C
authors.initials: CC
authors.lastname: Noronha
authors.forename: A I
authors.initials: AI
authors.lastname: Akinwande
authors.forename: A O
authors.initials: AO
authors.lastname: Okanlawon
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008550:Melatonin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013086:Spermatic Cord Torsion / Q000188:drug therapy* / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D008550:Melatonin