title: Human herpesvirus 7, Epstein-Barr virus and human cytomegalovirus in periodontal tissues of periodontally diseased and healthy subjects.
abstract: OBJECTIVE
  To evaluate (i) the presence of human herpesvirus 7 (HHV-7), Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV), and (ii) the transcription pattern of HHV-7 in gingival biopsies from patients affected by periodontitis (P) and periodontally healthy subjects (H).
  
  
  METHODS
  Thirty-seven subjects (P: n=24; H: n=13) were included. Each P patient contributed two gingival biopsies (representative of a clinically affected and non-affected site) and each H subject contributed one gingival biopsy. After DNA extraction, nested polymerase chain reaction was used to identify the viruses.
  
  
  RESULTS
  HHV-7 was detected in 91.7% of P patients and in 61.5% of H subjects (p=0.02), EBV in 50.0% samples of P patients and 7.7% of H subjects (p=0.005) and HCMV only in one sample from H group. EBV was more frequently detected in biopsies from affected sites (50.0%) than from non-affected sites (16.7%) (p=0.008). HHV-7 transcription was detected in 15.4% of affected and 15.4% of non-affected sites.
  
  
  CONCLUSIONS
  The results indicate that (i) gingival tissues can be considered a potential reservoir for HHV-7; (ii) when present, HHV-7 persists in a latent state in the majority of cases; (iii) the presence of EBV seems to be associated with the diseased state of the patient and site.
authors.affiliation: Section of Microbiology, Department of Experimental and Diagnostic Medicine, University of Ferrara, Ferrara, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Rotola
authors.forename: Enrico
authors.initials: E
authors.lastname: Cassai
authors.forename: Roberto
authors.initials: R
authors.lastname: Farina
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Caselli
authors.forename: Valentina
authors.initials: V
authors.lastname: Gentili
authors.forename: Tiziana
authors.initials: T
authors.lastname: Lazzarotto
authors.forename: Leonardo
authors.initials: L
authors.lastname: Trombelli
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000821:virology*; D001706:Biopsy; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D005881:Gingiva / Q000473:pathology / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D016199:Herpesvirus 7, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D018709:Statistics, Nonparametric
chemical_list: D004279:DNA, Viral