title: Vaccination with transforming growth factor-beta insensitive dendritic cells suppresses pulmonary metastases of renal carcinoma in mice.
abstract: Dendritic cells (DCs) have been widely used as cancer vaccines. However, their functional abilities have often been suppressed by tumor-secreted immunosuppressants such as transforming growth factor-beta (TGF-beta). We developed a new strategy using a TGF-beta insensitive DC as cancer vaccine. The effect of this vaccine was tested in a murine pulmonary metastases model of renal carcinoma (Renca). Tumor lysate-pulsed DCs (TP-DCs) were infected with retrovirus containing gene of dominant negative TGF-beta type II receptor (TbetaRIIDN) and thus made TGF-beta insensitive. Vaccination of the mice bearing Renca pulmonary metastases with the TbetaRIIDN TP-DC induced powerful tumor-specific cytotoxic T lymphocyte (CTL) responses, suppressed pulmonary metastases, and prolonged survival times. These results suggest TGF-beta-insensitive TP-DC vaccine can be used to enhance the antitumor efficacy of DC vaccine.
authors.affiliation: Department of Urology, Xijing Hospital, Fourth Military Medical University, 15 Chang Le West Road, Xi'an, Shaanxi Province 710032, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.forename: Longxin
authors.initials: L
authors.lastname: Wang
authors.forename: Weijun
authors.initials: W
authors.lastname: Qin
authors.forename: Fuli
authors.initials: F
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Song
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Weihong
authors.initials: W
authors.lastname: Wen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Kaichang
authors.initials: K
authors.lastname: Zhu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Chung
authors.initials: C
authors.lastname: Lee
authors.forename: Weide
authors.initials: W
authors.lastname: Zhong
authors.forename: Yinglu
authors.initials: Y
authors.lastname: Guo
authors.forename: He
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D007680:Kidney Neoplasms / Q000276:immunology / Q000473:pathology*; D008175:Lung Neoplasms / Q000276:immunology / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000276:immunology*
chemical_list: D016212:Transforming Growth Factor beta