Amino-acid limitation induces the GCN2 signaling pathway in myoblasts but not in myotubes.
There is a growing body of evidence that suggests that amino acids play an important role in controlling gene expression, but the cell specificity of the amino-acid-mediated regulation of gene expression in mammals remains unknown. Using a model of muscle cells (C2C12) at two stages of differentiation, i.e. myoblasts and myotubes, we employed transcriptional profiling to show that amino-acid deficiency does not regulate the same set of gene in differentiated and non-differentiated cells. Furthermore, in myotubes, the GCN2 pathway is not activated by amino-acid starvation due to an amino-acid supply from intracellular proteolysis associated with a low GCN2 expression.
Deval
Christiane
C
Unité de Nutrition Humaine, UMR 1019, INRA de Theix, Saint Genès Champanelle, France.
Talvas
Jérémie
J
Chaveroux
Cédric
C
Maurin
Anne-Catherine
AC
Mordier
Sylvie
S
Cherasse
Yoan
Y
Parry
Laurent
L
Carraro
Valérie
V
Jousse
Céline
C
Bruhat
Alain
A
Fafournoux
Pierre
P
D000596:Amino Acids / Q000172:deficiency*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D015870:Gene Expression; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000201:enzymology*; D032446:Myoblasts / Q000201:enzymology*; D020411:Oligonucleotide Array Sequence Analysis; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction; D014158:Transcription, Genetic
D000596:Amino Acids; C070634:Eif2ak4 protein, mouse; D017346:Protein Serine-Threonine Kinases