Transcriptional profiles in CD8+ T cells from HIV+ progressors on HAART are characterized by coordinated up-regulation of oxidative phosphorylation enzymes and interferon responses.
The functional impairment and numerical decline of CD8+ T cells during HIV infection has a profound effect on disease progression, but only limited microarray studies have used CD8+ T cells. To understand the interactions of HIV and host CD8+ T cells at different disease status, we used the Illumina Human-6 BeadChips to evaluate the transcriptional profile (>48,000 transcripts) in primary CD8+ T cells from HIV+ therapy-naive non-progressors and therapy-experienced progressors. 68 differentially expressed genes were identified, of which 6 have been reported in HIV context, while others are associated with biological functions relevant to HIV pathogenesis. By GSEA, the coordinated up-regulation of oxidative phosphorylation enzymes and interferon responses were detected as fingerprints in HIV progressors on HAART, whereas LTNP displayed a transcriptional signature of coordinated up-regulation of components of MAPK and cytotoxicty pathways. These results will provide biological insights into natural control of HIV versus HIV control under HAART.
Wu
Jing Qin
JQ
Retroviral Genetics Division, Center for Virus Research, Westmead Millennium Institute, University of Sydney, Westmead, NSW, Australia. jingqin_wu@wmi.usyd.edu.au
Dwyer
Dominic E
DE
Dyer
Wayne B
WB
Yang
Yee Hwa
YH
Wang
Bin
B
Saksena
Nitin K
NK
D023241:Antiretroviral Therapy, Highly Active; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D020869:Gene Expression Profiling; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007372:Interferons / Q000494:pharmacology / Q000627:therapeutic use*; D010085:Oxidative Phosphorylation; D014158:Transcription, Genetic / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects
D007372:Interferons