Adipose-derived mesenchymal stem cells in biosutures do not improve healing of experimental colonic anastomoses.
BACKGROUND
The feasibility of producing 'biosutures' coated with adipose-derived mesenchymal stem cells (ASCs) was assessed and their effect on colonic anastomoses evaluated.


METHODS
Sutures were cultured with ASCs isolated from BDIX rats before analysis by light and scanning electron microscopy. Anastomoses were performed in 40 BDIX rats using either biosutures or conventional sutures. Histopathological features, adhesion formation and anastomotic strength were evaluated.


RESULTS
Sutures were coated with ASCs within 24 h. Anastomoses made with biosutures had a lower adhesion index only during the first week (P = 0.006 at 7 days), but not subsequently. There were no significant differences in anastomotic healing with the two types of suture.


CONCLUSIONS
ASC biosutures have no effect on anastomotic healing in rats.
Pascual
I
I
Department of General Surgery, La Paz University Hospital, Madrid, Spain. isabelpasmi@hotmail.com
de Miguel
G Fernández
GF
Gómez-Pinedo
U A
UA
de Miguel
F
F
Arranz
M García
MG
García-Olmo
D
D
D000273:Adipose Tissue; D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000601:surgery*; D005240:Feasibility Studies; D059630:Mesenchymal Stem Cells; D051381:Rats; D013536:Suture Techniques; D013537:Sutures; D014945:Wound Healing