title: Using aptamers to study protein-protein interactions.
abstract: The emerging science of systems biology focuses on the systematic study of complex interactions in whole biological systems. A systemic, or integrative, methodology is employed as the chief means of discovering new properties and understanding the aggregate of processes that occur in a biological system. Accordingly, the Human Genome Project has provided a complete map of genes and resultant proteins corresponding to their function. Protein-protein interactions are important pieces of this biological tapestry, and understanding how they work cooperatively in a cell will result in a better understanding of the whole organism. To accomplish this objective, we report the use of DNA/RNA aptamers as a novel tool for the study and elucidation of protein-protein interactions, both in vivo and in vitro.
authors.affiliation: Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, Center for Research at the Bio/Nano Interface, University of Florida, Gainesville, FL 32611-7200, USA.
authors.forename: Parag
authors.initials: P
authors.lastname: Parekh
authors.forename: Jennifer
authors.initials: J
authors.lastname: Martin
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Dalia
authors.initials: D
authors.lastname: Colon
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide