title: Dp71f modulates GSK3-beta recruitment to the beta1-integrin adhesion complex.
abstract: Previously, it was shown that Dp71f binds to the beta1-integrin adhesion complex to modulate PC12 cell adhesion. The absence of Dp71f led to a failure in the beta1-integrin adhesion complex formation. One of the structural proteins which links the beta1-integrin cytoplasmic domain to the actin cytoskeleton is ILK. GSK3-beta is an ILK substrate and the carboxi-terminal region of dystrophin 427 is a substrate for hierarchical phosphorylation by GSK3-beta. Dp71f contains the carboxi-terminal domain present in dystrophin 427. By using co-immunoprecipitation assays, in the present work it is demonstrated that in the neuronal PC12 cell line an interaction between Dp71f and GSK3-beta occurs. This interaction was corroborated by in vitro pulldown assays. We show that GSK3-beta is recruited to the beta1-integrin complex and that a reduced expression of Dp71f induces a reduced GSK3-beta recruitment to the beta1-integrin complex. In addition, the present work establishes that adhesion of PC12 cells to laminin does not influence the phosphorylation status of Dp71f.
authors.affiliation: University Center of Biomedical Research, Universidad de Colima, Avenida 25 de Julio 965 Col. Villa San Sebastián, C.P. 28045, Colima, Colima, Mexico. joelcerna@ucol.mx
authors.forename: Joel Cerna
authors.initials: JC
authors.lastname: Cortés
authors.forename: Eliud Alfredo Garcia
authors.initials: EA
authors.lastname: Montalvo
authors.forename: Jesús
authors.initials: J
authors.lastname: Muñiz
authors.forename: Dominique
authors.initials: D
authors.lastname: Mornet
authors.forename: Efrain
authors.initials: E
authors.lastname: Garrido
authors.forename: Federico
authors.initials: F
authors.lastname: Centeno
authors.forename: Bulmaro
authors.initials: B
authors.lastname: Cisneros
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D016189:Dystrophin / Q000502:physiology*; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D019012:Integrin beta1 / Q000378:metabolism*; D007797:Laminin / Q000502:physiology; D016716:PC12 Cells; D010766:Phosphorylation; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats; D000099200:Scaffold Protein ILK
chemical_list: D000199:Actins; D016189:Dystrophin; D038362:Glycogen Synthase Kinase 3; D000071679:Glycogen Synthase Kinase 3 beta; D019012:Integrin beta1; D007797:Laminin; D017346:Protein Serine-Threonine Kinases; D000099200:Scaffold Protein ILK; C085339:apo-dystrophin 1