title: [Cloning and secretory expression of islet neogenesis-associated protein in Pichia pastoris].
abstract: OBJECTIVE
  To clone the recombinant human islet neogenesis-associated protein (rhINGAP) gene for its secretory expression in Pichia pastoris.
  
  
  METHODS
  INGAP gene was amplified with PCR and inserted between Xho I and EcoR I downstream sites of the alpha factor of the recombinant plasmid alpha/pUC18. The fusion gene of alpha factor and INGAP was subsequently inserted between BamH I and EcoR I sites of the plasmid pPIC9K of P. pastoris. After confirmation with restriction enzyme digestion and sequencing, the positive recombinant plasmid that integrated INGAP gene was linearized with Sal I digestion and transformed into the yeast host strain GS115 through electroporation. The yeast transformants that harbored the INGAP gene with high copies were selected with the auxotroph medium and G418, followed then by PCR verification of the positive transformants, from which the expression of recombinant human INGAP was induced with methanol as the only carbone source. The antigenic activity of the desired protein was then detected using Western blotting and enzyme-linked immunosorbent assay (ELISA).
  
  
  CONCLUSIONS
  The recombinant expression plasmid INGAP/pPIC9K was successfully constructed, and 3 positive transformants were obtained. The expressed protein showed good antigenic activity as confirmed by Western blotting and ELISA.
authors.affiliation: Department of Endocrinology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. shajianping99@sina.com
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Sha
authors.forename: Yao-ming
authors.initials: YM
authors.lastname: Xue
authors.forename: Xuan
authors.initials: X
authors.lastname: Chen
authors.forename: Zhan-jun
authors.initials: ZJ
authors.lastname: Zeng
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.forename: Xiang-rong
authors.initials: XR
authors.lastname: Luo
authors.forename: Fei-ying
authors.initials: FY
authors.lastname: He
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Feng-ting
authors.initials: FT
authors.lastname: Zhuo
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006801:Humans; D007515:Islets of Langerhans / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D011994:Recombinant Proteins