[Antitumor immunotherapy with the use of synthetic fragments of survivin].
The endogenous protein survivin is present in tumor cells and inhibits apoptosis. The influence of vaccination of mice by survivin fragments on growth of various types of tumors was studied to examine the possibility of creation of an antitumor vaccinating agent on its basis. Two peptides corresponding to the (118-144) and (80-88)-(153-165) sequences of survivin 2B were chosen and synthesized on the basis of literature data and theoretical calculations. Their ability to stimulate antibody production in mice of the C57BL/6J line (b haplotype) and in BDF1 hybrids (b x d haplotype) was investigated. Both peptides were shown to stimulate production of antibodies that bound the recombinant survivin in the BDF1 mice. Immunization of the BDF1 and C57BL/6J mice with the recombinant survivin resulted in the formation of antibodies that reacted with the (118-144) peptide. The effect of preventive vaccination with the peptides and the recombinant protein on the dynamics of growth of several species of tumors was studied. Vaccination with the (80-88)-(153-165) peptide was found to cause an antitumor effect in BDF1 mice suffering from sarcoma S-37. Thus, the creation of an antitumor agent on the basis of this peptide is a promising area of further studies.
Volkova
T D
TD
Koroev
D O
DO
Titova
M A
MA
Oboznaia
M B
MB
Filatova
M P
MP
Pankratov
A A
AA
Morozova
N B
NB
Zolotavkina
Iu B
IuB
Iakubovskaia
R I
RI
Vol'pina
O M
OM
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D018984:Epitopes, T-Lymphocyte; D006801:Humans; D007167:Immunotherapy; D051034:Inhibitor of Apoptosis Proteins; D051379:Mice; D008815:Mice, Inbred Strains; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology / Q000517:prevention & control*; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D013045:Species Specificity; D000077022:Survivin; D023041:Xenograft Model Antitumor Assays
C107489:BIRC5 protein, human; D018984:Epitopes, T-Lymphocyte; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D000077022:Survivin