title: In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression.
abstract: In order to assess the role of osteopontin (OPN) in leukocyte accumulation in inflammatory conditions, native OPN and its thrombin cleaved form (OPN+Thr) were studied in vivo using a rodent subcutaneous air pouch model (AP). Both forms of OPN-induced macrophage infiltration into the AP in wild-type mice. In animals lacking CD44, macrophage numbers were significantly reduced within the cavity, but cells still accumulated along the subcutaneous lining. In animals lacking endogenous OPN, no differences were found in exogenous OPN-induced macrophage accumulation, although macrophage exhibited increased alpha4 integrin expression. These studies reveal that both OPN and OPN+Thr attract macrophages in vivo through CD44.
authors.affiliation: Molecular and Integrative Neurosciences Department, The Scripps Research Institute, La Jolla, CA 92037, USA. cmarcond@scripps.edu
authors.forename: Maria Cecilia G
authors.initials: MC
authors.lastname: Marcondes
authors.forename: Matthew
authors.initials: M
authors.lastname: Poling
authors.forename: Debbie D
authors.initials: DD
authors.lastname: Watry
authors.forename: DeShon
authors.initials: D
authors.lastname: Hall
authors.forename: Howard S
authors.initials: HS
authors.lastname: Fox
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005434:Flow Cytometry; D018960:Hyaluronan Receptors / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D053495:Osteopontin / Q000276:immunology* / Q000378:metabolism
chemical_list: D018960:Hyaluronan Receptors; D053495:Osteopontin