Hepatic differentiation of cord blood-derived multipotent progenitor cells (MPCs) in vitro.
Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells that possesses practical and ethical advantages. We previously reported a novel UCB-derived adult stem cells which we termed umbilical cord blood-derived multipotent progenitor cells' (MPCs). MPCs were capable of differentiating into functional neuronal cells. Under appropriate conditions lasting several days or weeks, we now show that the MPCs differentiate into hepatocyte-like cells in vitro; their properties were verified using reverse transcription-polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, periodic acid-Schiff (PAS) staining of accumulated glycogen and an enzyme-linked immunosorbent assay (ELISA). We also found that hepatic differentiated cells expressed hepatocyte specific markers, such as albumin, hepatocyte nuclear factor (HNF)-1alpha, HNF4, cytokeratin (CK)-8, CK-18, tyrosine amino transferase (TAT), and CYP2B6. Moreover, albumin was secreted, which suggests that MPCs from UCB possess multi-differentiation potential and have the capacity to differentiate into functional cells of hepatic lineage in vitro.
Moon
Young Joon
YJ
Department of Life Science, College of Natural Science, Chung-Ang University, Seoul, Republic of Korea.
Lee
Myoung Woo
MW
Yoon
Hee Hoon
HH
Yang
Mal Sook
MS
Jang
In Keun
IK
Lee
Jong Eun
JE
Kim
Hyo Eun
HE
Eom
Young-woo
YW
Park
Joon Seong
JS
Kim
Hugh C
HC
Kim
Young Jin
YJ
Lee
Kwang-Ho
KH
D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D048430:Cell Shape; D002478:Cells, Cultured; D002648:Child; D017077:Culture Media, Conditioned; D005312:Fetal Blood / Q000166:cytology*; D051519:Fibroblast Growth Factor 4 / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000502:physiology*; D039902:Multipotent Stem Cells / Q000166:cytology / Q000502:physiology*
D015415:Biomarkers; D017077:Culture Media, Conditioned; C496445:FGF4 protein, human; D051519:Fibroblast Growth Factor 4