Proteome analysis of human dorsolateral prefrontal cortex using shotgun mass spectrometry.
The prefrontal cortex executes important functions such as differentiation of conflicting thoughts, correct social behavior and personality expression, and is directly implicated in different neurodegenerative diseases. We performed a shotgun proteome analysis that included IEF fractionation, RP-LC, and MALDI-TOF/TOF mass spectrometric analysis of tryptic digests from a pool of seven human dorsolateral prefrontal cortex protein extracts. In this report, we present a catalog of 387 proteins expressed in these samples, identified by two or more peptides and high confidence search scores. These proteins are involved in different biological processes such as cell growth and/or maintenance, metabolism/energy pathways, cell communication/signal transduction, protein metabolism, transport, regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolism, and immune response. This analysis contributes to the knowledge of the human brain proteome by adding sample diversity and protein expression data from an alternative technical approach. It will also aid comparative studies of different brain areas and medical conditions, with future applications in basic and clinical research.
Martins-de-Souza
Daniel
D
Laboratório de Neurociências, Instituto de Psiquiatria, Facultdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil. danms90@gmail.com
Gattaz
Wagner F
WF
Schmitt
Andrea
A
Rewerts
Christiane
C
Maccarrone
Giuseppina
G
Dias-Neto
Emmanuel
E
Turck
Christoph W
CW
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D016208:Databases, Factual; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008875:Middle Aged; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D017397:Prefrontal Cortex / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
D010455:Peptides; D020543:Proteome