Preimplantation HLA haplotyping using tri-, tetra-, and pentanucleotide short tandem repeats for HLA matching.
OBJECTIVE
To aid couples wishing to conceive children who are HLA matched to a sibling in need of a hematopoietic progenitor cell transplant, we developed a preimplantation HLA haplotype analysis of embryos that utilizes tri-, tetra-, and pentanucleotide STR markers.


METHODS
For preimplantation HLA genotyping, we use polymorphic STR markers located across the HLA and flanking regions, selecting exclusively tri-, tetra-, and pentanucleotide repeats. These markers can be resolved using either capillary electrophoresis (CE) or polyacrylamide gels.


RESULTS
We have developed 43 reliable STR markers for preimplantation HLA matching. Selected STR markers enabled unambiguous identification of embryos whose HLA haplotypes were matched with the affected patient using polyacrylamide gel or capillary electrophoresis.


CONCLUSIONS
The use of tri-, tetra-, and pentanucleotide repeat markers and polyacrylamide gels for STR genotyping in HLA matching is a simple and cost effective approach to clinical testing.
Bick
Sarah L
SL
Division of Medical Genetics, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Bick
David P
DP
Wells
Brent E
BE
Roesler
Mark R
MR
Strawn
Estil Y
EY
Lau
Eduardo C
EC
D017690:Cell Transplantation; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D010064:Embryo Implantation; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006680:HLA Antigens / Q000737:chemistry / Q000235:genetics*; D006239:Haplotypes; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D009711:Nucleotides / Q000737:chemistry*; D019836:Preimplantation Diagnosis / Q000379:methods
D005819:Genetic Markers; D006680:HLA Antigens; D009711:Nucleotides