Differential expression of angiogenic factors in peripheral nerve sheath tumors.
It is difficult to differentiate some malignant peripheral nerve sheath tumors (MPNST) from benign peripheral nerve sheath tumors (BPNST) histologically, and to predict the clinical outcome of patients with MPNST. In this study, the expression of VEGF and MVD were evaluated immunohistochemically in 22 cases of MPNST, 14 of neurofibroma and 19 of schwannoma and correlation of the staining grade of VEGF or MVD and the various clinical factors were analyzed, and statistically evaluated. Levels of VEGF mRNA expression were also determined with real-time RT-PCR. Statistically higher positive staining for VEGF was observed in MPNST compared to neurofibroma (P=0.004) and schwannoma (P<0.001). Even low grade MPNST showed higher VEGF positive staining than neurofibroma. Moreover, high VEGF expression statistically correlated with the poor prognosis of the patients with MPNST (P=0.015). Although MVD in MPNST was significantly higher than that in neurofibroma (P=0.038) and schwannoma (P<0.001), MVD could not predict the prognosis of the patients with MPNST. Although VEGF mRNA expression tended to be higher in MPNST compared to neurofibroma, the difference was not significant. Levels of VEGF protein expression serve as a novel diagnostic and prognostic tools for peripheral nerve sheath tumors.
Wasa
Junji
J
Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, 65-Tsuruma, Showa, Nagoya 466-8550, Japan.
Nishida
Yoshihiro
Y
Suzuki
Yoshitaka
Y
Tsukushi
Satoshi
S
Shido
Yoji
Y
Hosono
Kozo
K
Shimoyama
Yoshie
Y
Nakamura
Shigeo
S
Ishiguro
Naoki
N
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018317:Nerve Sheath Neoplasms / Q000098:blood supply / Q000737:chemistry*; D009442:Neurilemmoma / Q000098:blood supply / Q000737:chemistry; D009455:Neurofibroma / Q000098:blood supply / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A