title: Transcriptional analysis of diverse strains Mycobacterium avium subspecies paratuberculosis in primary bovine monocyte derived macrophages.
abstract: In this study we analyzed the macrophage-induced gene expression of three diverse genotypes of Mycobacterium avium subsp. paratuberculosis (MAP). Using selective capture of transcribed sequences (SCOTS) on three genotypically diverse MAP isolates from cattle, human, and sheep exposed to primary bovine monocyte derived macrophages for 48 h and 120 h we created and sequenced six cDNA libraries. Sequence annotations revealed that the cattle isolate up-regulated 27 and 241 genes; the human isolate up-regulated 22 and 53 genes, and the sheep isolate up-regulated 35 and 358 genes, at the two time points respectively. Thirteen to thirty-three percent of the genes identified did not have any annotated function. Despite variations in the genes identified, the patterns of expression fell into overlapping cellular functions as inferred by pathway analysis. For example, 10-12% of the genes expressed by all three strains at each time point were associated with cell-wall biosynthesis. All three strains of MAP studied up-regulated genes in pathways that combat oxidative stress, metabolic and nutritional starvation, and cell survival. Taken together, this comparative transcriptional analysis suggests that diverse MAP genotypes respond with similar modus operandi for survival in the host.
authors.affiliation: Veterinary Population Medicine, University of Minnesota, 1365 Gortner Avenue, 225 VMC, Saint Paul, MN 55108, USA.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Zhu
authors.forename: Zheng J
authors.initials: ZJ
authors.lastname: Tu
authors.forename: Paul M
authors.initials: PM
authors.lastname: Coussens
authors.forename: Vivek
authors.initials: V
authors.lastname: Kapur
authors.forename: Harish
authors.initials: H
authors.lastname: Janagama
authors.forename: Saleh
authors.initials: S
authors.lastname: Naser
authors.forename: Srinand
authors.initials: S
authors.lastname: Sreevatsan
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D015723:Gene Library; D005838:Genotype; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D009000:Monocytes / Q000382:microbiology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010283:Paratuberculosis / Q000382:microbiology; D012756:Sheep; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins