title: Expression of endothelial monocyte-activating polypeptide II in the rat dental follicle and its potential role in tooth eruption.
abstract: Endothelial monocyte-activating polypeptide II (EMAP-II) is an inflammatory cytokine with chemotactic activity. Because the dental follicle (DF) recruits mononuclear cells (osteoclast precursors) to promote the osteoclastogenesis needed for tooth eruption, it was the aim of this study to determine if EMAP-II contributes to this recruitment. Using a DNA microarray, EMAP-II was found to be highly expressed in vivo in the DFs of day 1 to day 11 postnatal rats, with its expression elevated on days 1 and 3. Use of a short interfering RNA (siRNA) to knock down EMAP-II expression resulted in a reduction in the expression of colony-stimulating factor-1 (CSF-1) and monocyte chemoattractant protein-1 (MCP-1) in the DF cells. Addition of EMAP-II protein to the DF cells partially restored the expression of CSF-1 and MCP-1. In chemotaxis assays using either conditioned medium of the DF cells with anti-(EMAP-II) immunoglobulin G added or conditioned medium of DF cells with EMAP-II knocked down by siRNA, migration indexes of bone marrow mononuclear cells were significantly reduced. These results suggest that EMAP-II is another chemotactic molecule in the dental follicle involved in the recruitment of mononuclear cells, and that EMAP-II may exert its chemotactic function directly by recruiting mononuclear cells and indirectly by enhancing the expression of other chemotactic molecules (CSF-1 and MCP-1).
authors.affiliation: Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Dawen
authors.initials: D
authors.lastname: Liu
authors.forename: Gary E
authors.initials: GE
authors.lastname: Wise
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis; D002634:Chemotaxis, Leukocyte; D016207:Cytokines / Q000096:biosynthesis* / Q000502:physiology*; D003795:Dental Sac / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000502:physiology; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D010010:Osteoclasts / Q000502:physiology; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014078:Tooth Eruption / Q000502:physiology*
chemical_list: D018932:Chemokine CCL2; D016207:Cytokines; D009363:Neoplasm Proteins; D016601:RNA-Binding Proteins; C077219:small inducible cytokine subfamily E, member 1; D016173:Macrophage Colony-Stimulating Factor