The dynamics of the nitric oxide release-transient from stretched muscle cells.
Potent nitric oxide (NO) signals are described for many forms of cell-cell communication. Although NO plays a significant role in skeletal muscle metabolism and contractility and in precursor activation during muscle formation and stretching, there is no direct evidence of stretch-induced NO release from muscle. Differentiated muscle cell cultures from normal and dystrophic mdx mice were preloaded with the NO-specific dye DAF-2 (diaminofluorescein-2) before stretching. NO release was detected by video-microscopy. NO was released rapidly from wild-type (WT) cells after stretch and intensity declined rapidly to a plateau. Mdx cells showed much less NO release. Direct observations of the time-course of stretch-induced NO release in WT cells is congruent with the hypothesis of NO-mediated stretch activation of satellite cells in normal skeletal muscle. Distinct differences in the time-course between normal and dystrophic cells indicate visualization methods for NO release will be a sensitive measure of NOS-1 restoration following diverse treatment approaches to muscular dystrophy.
Wozniak
A C
AC
Department of Human Anatomy and Cell Science, Faculty of Medicine, The University of Manitoba, Canada.
Anderson
J E
JE
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D016189:Dystrophin / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D025202:Molecular Diagnostic Techniques; D020388:Muscular Dystrophy, Duchenne / Q000175:diagnosis* / Q000473:pathology; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000235:genetics / Q000378:metabolism*; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism* / Q000473:pathology; D013234:Stem Cells / Q000473:pathology; D013314:Stress, Mechanical; D013997:Time Factors
D016189:Dystrophin; D009569:Nitric Oxide; D052248:Nitric Oxide Synthase Type I; C496321:Nos1 protein, mouse