title: The kinesin I family member KIF5C is a novel substrate for protein kinase CK2.
abstract: Protein kinase CK2 is ubiquitously expressed. The holoenzyme is composed of two catalytic alpha- or alpha'-subunits and two regulatory beta-subunits but evidence is accumulating that the subunits can function independently. The composition of the holoenzyme as well as the expression of the individual subunits varies in different tissues, with high expression of CK2alpha' in testis and brain. CK2 phosphorylates a number of different substrates which are implicated in basal cellular processes such as proliferation and survival of cells. Here, we report a new substrate, KIF5C, which is a member of the kinesin 1 family of motor neuron proteins. Phosphorylation of KIF5C was demonstrated in vitro and in vivo. Using deletion mutants, a peptide library, and mutation analysis a phosphorylation site for CK2 was mapped to amino acid 338 which is located in the non-motor domain of KIF5C. Interestingly, KIF5C is phosphorylated by holoenzymes composed of CK2alpha/CK2beta and CK2alpha'/CK2beta as well as by CK2alpha' alone but not by CK2alpha alone.
authors.affiliation: Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Gebäude 44, 66424 Homburg, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Schäfer
authors.forename: Claudia
authors.initials: C
authors.lastname: Götz
authors.forename: Mathias
authors.initials: M
authors.lastname: Montenarh
mesh_terms: D000595:Amino Acid Sequence; D047390:Casein Kinase II / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D019151:Peptide Library; D010766:Phosphorylation; D013379:Substrate Specificity
chemical_list: D019151:Peptide Library; D047390:Casein Kinase II; C514435:KIF5C protein, human; D016547:Kinesins