title: Deficit of Kcnma1 mRNA expression in the dentate gyrus of epileptic rats.
abstract: Epileptogenesis in mesial temporal lobe epilepsy is determined by several factors including abnormalities in the expression and function of ion channels. Here, we report a long-lasting deficit in gene expression of Kcnma1 coding for the large-conductance calcium-activated potassium (BK, MaxiK) channel alpha-subunits after pilocarpine-induced status epilepticus. By using comparative real-time PCR, Taqman gene expression assays, and the delta-delta comparative threshold method we detected a significant reduction in Kcnma1 expression in microdissected dentate gyrus at different intervals after status epilepticus (24 h, 10 days, 1 month, and more than 2 months). BK channels are key regulators of neuronal excitability and transmitter release. Hence, defective Kcnma1 expression may play a critical role in the pathogenesis of mesial temporal lobe epilepsy.
authors.affiliation: Department of Biological Sciences, The University of Texas, Brownsville/Texas Southmost College, Brownsville, Texas, USA.
authors.forename: Boris
authors.initials: B
authors.lastname: Ermolinsky
authors.forename: Massoud F
authors.initials: MF
authors.lastname: Arshadmansab
authors.forename: Luis F
authors.initials: LF
authors.lastname: Pacheco Otalora
authors.forename: Masoud M
authors.initials: MM
authors.lastname: Zarei
authors.forename: Emilio R
authors.initials: ER
authors.lastname: Garrido-Sanabria
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D018891:Dentate Gyrus / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D004833:Epilepsy, Temporal Lobe / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits / Q000235:genetics*; D018721:Muscarinic Agonists / Q000008:administration & dosage / Q000633:toxicity; D010862:Pilocarpine / Q000008:administration & dosage / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012640:Seizures / Q000139:chemically induced / Q000235:genetics; D013226:Status Epilepticus / Q000139:chemically induced / Q000235:genetics; D013997:Time Factors
chemical_list: C494842:Kcnma1 protein, rat; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D018721:Muscarinic Agonists; D012333:RNA, Messenger; D010862:Pilocarpine