title: Influence of Epstein-Barr virus infection in adult T-cell leukemia.
abstract: The involvement of adult T-cell leukemia (ATL) cells in organs such as the skin and lymph nodes is observed in about 50% of cases of ATL. Epstein-Barr virus (EBV) infection has often been observed in the clinical course of ATL. In this study, we established two B-cell lines from peripheral blood of patients with ATL. EBV DNA, proviral DNA for HTLV-1 and Tax mRNA were detected in both lines. As part of the characterization of these cells, an enhanced expression of intercellular adhesion molecule-1 (ICAM-1) (CD54) or ICAM-3 (ICAM-3) (CD50), lymphocyte function-1 (LFA-1) (CD11a/CD18), and Mac-1 (CD11b/CD18) was observed. To investigate the role of the interaction of these viruses, we transfected EBV and/or HTLV-1 into a healthy donor's lymphocytes, an EBV-infected B cell line, Raji, and a HTLV-1 negative T-cell line, Jurkat. Enhanced expression of adhesion molecules was also observed in double transfectants (EBV and HTLV-1). In the clinical course of ATL, LMP-1, EBNA-2, CD50 and CD54 were detected in lymph nodes and skin specimens by immunohistochemical staining. Furthermore, high levels of interleukin-4 (IL-4) were detected in these cell lines and transfectants. The results indicated that coinfection with HTLV-1 and EBV may induce aggressive organ involvement through the enhanced expression of adhesion molecules via IL-4 signaling. A new mechanism of ATL involvement is discussed.
authors.affiliation: Division of Hematology, Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Ueda
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Maeda
authors.forename: Terufumi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hanamoto
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Hijikata
authors.forename: Miyako
authors.initials: M
authors.lastname: Tanaka
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Takai
authors.forename: Chikara
authors.initials: C
authors.lastname: Hirase
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Morita
authors.forename: Akihisa
authors.initials: A
authors.lastname: Kanamaru
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000235:genetics; D001402:B-Lymphocytes / Q000473:pathology / Q000821:virology; D015815:Cell Adhesion Molecules / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D020031:Epstein-Barr Virus Infections / Q000150:complications*; D016356:Gene Products, tax / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000150:complications* / Q000235:genetics / Q000473:pathology; D016177:Macrophage-1 Antigen / Q000235:genetics; D011533:Proviruses / Q000235:genetics
chemical_list: D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D016356:Gene Products, tax; C483616:ICAM3 protein, human; D016177:Macrophage-1 Antigen; C514970:tax protein, Human T-lymphotrophic virus 1; D018799:Intercellular Adhesion Molecule-1