title: Effect of Ca2+ gradient on the structure of sarcoplasmic reticulum membranes.
abstract: The structure of sarcoplasmic reticulum membranes was studied in the presence of modeled transmembrane Ca2+ gradient corresponding to the status of Ca2+ depot at different stages of the muscle contraction-relaxation cycle in health and disease. Various sites of the membrane were characterized using spectral analysis of tryptophan, pyrene, and merocyanine-540 fluorescence without evaluating specific changes in the molecules of membrane components (Ca2+ -ATPase, ryanodine receptor, and lipids). The transmembrane Ca2+ gradient modulates the protein-lipid interactions and structural characteristics of the membrane. The proposed model can be used for studies of the effects of pharmacologically active substances and endogenous regulators.
authors.affiliation: N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow. olgavek@yandex.ru
authors.forename: O M
authors.initials: OM
authors.lastname: Vekshina
authors.forename: Yu A
authors.initials: YA
authors.lastname: Kim
authors.forename: N L
authors.initials: NL
authors.lastname: Vekshin
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D005453:Fluorescence; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D008962:Models, Theoretical; D011485:Protein Binding; D011721:Pyrenes / Q000737:chemistry / Q000378:metabolism; D011744:Pyrimidinones / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism
chemical_list: D008563:Membrane Lipids; D011721:Pyrenes; D011744:Pyrimidinones; D019837:Ryanodine Receptor Calcium Release Channel; C003954:merocyanine dye; D014364:Tryptophan; C030984:pyrene; D000252:Calcium-Transporting ATPases; D002118:Calcium