title: Expression of the cytochrome P450 epoxygenase CYP2J2 in human monocytic leukocytes.
abstract: CYP2J2 is one of the cytochrome P450 epoxygenases involved in the metabolism of arachidonic acid. CYP2J2 has been identified in several tissues, especially cardiovascular tissues. CYP2J2 has cardiovascular effects, as epoxyeicosatrienoic acid, one of its metabolites, has anti-inflammatory and vasodilative activities. We investigated the expression of CYP2J2 in human leukocytes using reverse transcription-polymerase chain reaction, immunoblotting and immunostaining. Human monocytic cells, but not human neutrophils, exhibited constitutive expression of CYP2J2. Furthermore, the expression of CYP2J2 mRNA increased when the human monocytic cell line THP-1 cells and human monocytes were stimulated with phorbol 12-myristate 13-acetate and macrophage-colony stimulating factor in combination with granulocyte/macrophage-colony stimulating factor, respectively. These results suggest that expression of CYP2J2 was up-regulated when human monocytes differentiated into macrophages and that human monocytic cells and macrophages have a pathway to metabolize arachidonic acid using CYP epoxygenases.
authors.affiliation: Department of Cardiovascular and Renal Medicine, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Kaeko
authors.initials: K
authors.lastname: Nakayama
authors.forename: Takeaki
authors.initials: T
authors.lastname: Nitto
authors.forename: Teruo
authors.initials: T
authors.lastname: Inoue
authors.forename: Koichi
authors.initials: K
authors.lastname: Node
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D002460:Cell Line; D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D009000:Monocytes / Q000201:enzymology*; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism
chemical_list: C000717350:CYP2J2 protein, human; D016207:Cytokines; D016718:Arachidonic Acid; D003577:Cytochrome P-450 Enzyme System; D000090762:Cytochrome P-450 CYP2J2; D013755:Tetradecanoylphorbol Acetate