[Expression of COL1a1 and COL3a1 in the capsule of children with developmental dislocation of the hip].
OBJECTIVE
The etiology of developmental dislocation of the hip (DDH) remains uncertain, but some research has shown that this disorder is closely related to hip joint laxity. This study examined the expression of collagens type I and III mRNA and protein in the hip capsule of children with DDH in order to investigate the roles of collagens type I and III in hip joint laxity.


METHODS
Nine children with DDH and nine age and gender-matched normal children (control group) were enrolled. Semiquantitative RT-PCR method was used to detect mRNA expression of COL1a1 and COL3a1 in the hip capsule. Western-Blot method was used to detect protein expression of COL1a1 and COL3a1 in the hip capsule. The quantitative analysis of the COL1a1 and COL3a1 was performed by professional image software and the results were analyzed with standard statistical methods.


RESULTS
mRNA and protein expression of COL1a1 in the DDH group was significantly lower than that in the control group (P<0.01). Compared with the control group, COL1a3 mRNA expression in the DDH group decreased significantly (P<0.01), but COL1a3 protein expression was not significantly different.


CONCLUSIONS
The decreased collagen I mRNA and protein expression in the hip capsule might contribute to hip joint laxity in children with DDH. Collagen type III may not be associated with hip joint laxity in DDH.
Wang
En-Bo
EB
Department of Pediatric Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China.
Zhao
Qun
Q
Li
Lian-Yong
LY
Shi
Li-Wei
LW
Gao
Hong
H
D015153:Blotting, Western; D002648:Child; D002657:Child Development; D002675:Child, Preschool; D024042:Collagen Type I / Q000032:analysis / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D024061:Collagen Type III / Q000032:analysis / Q000235:genetics*; D005260:Female; D006617:Hip Dislocation / Q000378:metabolism*; D006801:Humans; D007223:Infant; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
C439971:COL3A1 protein, human; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D024061:Collagen Type III; D012333:RNA, Messenger