Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues.
We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3%) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsatellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and progression of GBMs.
Kuga
Daisuke
D
Department of Neurosurgery, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Mizoguchi
Masahiro
M
Guan
Yanlei
Y
Hata
Nobuhiro
N
Yoshimoto
Koji
K
Shono
Tadahisa
T
Suzuki
Satoshi O
SO
Kukita
Yoji
Y
Tahira
Tomoko
T
Nagata
Shinji
S
Sasaki
Tomio
T
Hayashi
Kenshi
K
D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics*; D002648:Child; D005260:Female; D018628:Gene Dosage; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007834:Lasers; D019656:Loss of Heterozygosity; D008297:Male; D042282:Microdissection; D018895:Microsatellite Repeats; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction