title: cDNA clone, prokaryotic expression and purification of human interleukin-13 receptor [alpha]2 chain.
abstract: Despite advances in surgical technology and radiation therapy, the prognosis in the patients with malignant glioma remains poor. Recent studies show that interleukin-13 receptor [alpha]2 chain (IL-13Ra2), a brain tumor-associated receptor for IL-13, may play a role in immunotherapy for glioblastoma. We thus amplified human IL-13Ra2 gene from the human glioblastoma cell line using RT-PCR and cloned the target gene into the pET-28a, a prokaryotic expressing plasmid. After transformation, the recombinant plasmid expressed a soluble protein induced by IPTG. The purified recombinant protein was shown to be a single band on the SDS-PAGE with a predicated molecular weight of human IL-13Ra2 gene, suggesting that the recombinant protein of human IL-13Ra2 was successfully expressed. Recombinant IL-13Ra2 protein can be used as an anti-tumor vaccine, which may provide a promising new strategy for the treatment of brain malignant gliomas.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Yu
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Su
authors.forename: Zhebao
authors.initials: Z
authors.lastname: Wu
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Mao
authors.forename: Weiming
authors.initials: W
authors.lastname: Zheng
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000951:Antigens, Neoplasm / Q000737:chemistry; D001932:Brain Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D053663:Interleukin-13 Receptor alpha2 Subunit / Q000235:genetics* / Q000378:metabolism; D008954:Models, Biological; D008970:Molecular Weight; D010957:Plasmids / Q000378:metabolism; D013997:Time Factors
chemical_list: D000951:Antigens, Neoplasm; D018076:DNA, Complementary; D053663:Interleukin-13 Receptor alpha2 Subunit