An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region.
Myosin XI, a class of myosins expressed in plants is believed to be responsible for cytoplasmic streaming and the translocation of organelles and vesicles. To gain further insight into the translocation of organelles and vesicles by myosin XI, an isoform of Arabidopsis myosin XI, MYA2, was chosen and its role in peroxisome targeting was examined. Using the yeast two-hybrid screening method, two small GTPases, AtRabD1 and AtRabC2a, were identified as factors that interact with the C-terminal tail region of MYA2. Both recombinant AtRabs tagged with His bound to the recombinant C-terminal tail region of MYA2 tagged with GST in a GTP-dependent manner. Furthermore, AtRabC2a was localized on peroxisomes, when its CFP-tagged form was expressed transiently in protoplasts prepared from Arabidopsis leaf tissue. It is suggested that MYA2 targets the peroxisome through an interaction with AtRabC2a.
Hashimoto
Kohsuke
K
Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo 678-1279, Japan.
Igarashi
Hisako
H
Mano
Shoji
S
Takenaka
Chikako
C
Shiina
Takashi
T
Yamaguchi
Masatoshi
M
Demura
Taku
T
Nishimura
Mikio
M
Shimmen
Teruo
T
Yokota
Etsuo
E
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020675:Peroxisomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; C504143:MYA2 protein, Arabidopsis; D020033:Protein Isoforms; C533032:RabD1 protein, Arabidopsis; D018995:Myosin Heavy Chains; D020559:Monomeric GTP-Binding Proteins; C505651:RAC2 protein, Arabidopsis