title: Molecular profiles: a new tool to substantiate serum banks for evaluation of potential allergenicity of GMO.
abstract: Assessment of the allergenicity of GMOs involves performing a test with a panel of sera obtained from allergic donors. However, there is no clear indication of how to characterize the above-mentioned panel. The patient selection criteria should take into account the geographical location of patients, the intensity and nature of the environmental allergens in the area and the potential cross-reactivity among allergenic molecules. Sera for serum banks, obtained from patients with demonstrated food allergy, should be subjected to a further characterization by screening with a panel of relevant allergenic molecules. A representative panel of these sera should be used in the allergenicity assessment. Finally, the "in vitro" methodologies should have the adequate specificity and sensitivity, and the integrity of the molecules tested should be guaranteed.
authors.affiliation: Departamento de I+D, ALK-Abelló S.A., C/Miguel Fleta 19, E-28037 Madrid, Spain. domingo.barber@alk-abello.com
authors.forename: D
authors.initials: D
authors.lastname: Barber
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez
authors.forename: G
authors.initials: G
authors.lastname: Salcedo
mesh_terms: D000485:Allergens / Q000032:analysis / Q000276:immunology*; D000918:Antibody Specificity; D005512:Food Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D030841:Food, Genetically Modified; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D030821:Plants, Genetically Modified / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000485:Allergens; D007106:Immune Sera; D007073:Immunoglobulin E