title: Lack of association of endothelial nitric oxide synthase polymorphisms and migraine.
abstract: OBJECTIVE
  The aim of this study was to evaluate if 2 functional endothelial nitric oxide synthase (eNOS) gene polymorphisms might be risk factors for migraine.
  
  
  BACKGROUND
  Nitric oxide synthase promotes the synthesis of nitric oxide (NO). NO is a potent vasodilator and mediates several processes involved in migraine pathophysiology. Only one study has suggested an association with migraine with aura.
  
  
  METHODS
  We performed a sex- and age-matched case-control study using 2 eNOS polymorphisms (rs1800779 and rs1799983), which are in linkage disequilibrium. Genotypes were obtained with allele-specific probes in a real-time polymerase chain reaction assay. Genotypic and allelic distributions were compared with chi(2) method. We also estimated the reconstructed haplotypes and calculated ORs for individual haplotypes.
  
  
  RESULTS
  A total of 337 migraine patients (188 with aura) and 341 healthy controls were recruited. We found no significant differences in the distribution of genotypes and alleles for either polymorphism among clinical subgroups. Neither rs1800779 nor rs1799983 polymorphisms increased the risk for suffering from migraine aura.
  
  
  CONCLUSIONS
  As others have previously reported, we failed to demonstrate genetic association of the eNOS gene with migraine.
authors.affiliation: Services of Neurology, University Hospital Marqués de Valdecilla, Santander, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Toriello
authors.forename: Agustín
authors.initials: A
authors.lastname: Oterino
authors.forename: Julio
authors.initials: J
authors.lastname: Pascual
authors.forename: Jesús
authors.initials: J
authors.lastname: Castillo
authors.forename: Rafael
authors.initials: R
authors.lastname: Colás
authors.forename: Ana
authors.initials: A
authors.lastname: Alonso-Arranz
authors.forename: Carlos
authors.initials: C
authors.lastname: Ruiz-Alegría
authors.forename: Estrella
authors.initials: E
authors.lastname: Quintela
authors.forename: Fernando
authors.initials: F
authors.lastname: Montón
authors.forename: Nuria
authors.initials: N
authors.lastname: Ruiz-Lavilla
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D008040:Genetic Linkage / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D008881:Migraine Disorders / Q000201:enzymology* / Q000235:genetics*; D020325:Migraine with Aura / Q000201:enzymology / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D055815:Young Adult
chemical_list: D052250:Nitric Oxide Synthase Type III