High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection.
We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5-8 megabase sub-chromosomal region.
Fuhrman
Laura E
LE
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America.
Shianna
Kevin V
KV
Aballay
Alejandro
A
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000382:microbiology*; D004064:Digestive System / Q000382:microbiology; D004198:Disease Susceptibility; D004926:Escherichia coli / Q000302:isolation & purification; D005784:Gene Amplification; D020022:Genetic Predisposition to Disease; D016296:Mutagenesis; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D019779:Salmonella enterica / Q000302:isolation & purification