title: The appearance of viral antigen and antibody in the trachea of naive and vaccinated chickens infected with infectious laryngotracheitis virus.
abstract: In chickens vaccinated with SA-2 infectious laryngotracheitis (ILT) virus, viral antigen could no longer be detected in tracheal washings from day 7 post infection (pi). Total specific antibody was detected in tracheal washings from day 5 pi, IgA antibody appeared at day 6 pi, but neutralising antibody could not be detected until day 14. In the serum of vaccinated chickens, total antibody appeared on day 5 pi and neutralising antibody on day 7. However, no IgA antibody could be detected in serum. There was a substantial increase in the numbers of IgA- and IgG-synthesising cells in the trachea by day 3 pi, with a marked increase in the numbers of IgA-positive cells at day 7 pi. Following challenge with virulent CSW-1 ILT virus, no virus could be detected in the trachea of vaccinated chickens. There was also no evidence of an anamnestic antibody response in the trachea or in serum up to day 10 post challenge, and there was no significant change in the numbers of IgA- or IgG-synthesising cells in the tracheas of vaccinated chickens up to day 7 post challenge.
authors.affiliation: CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.
authors.forename: J J
authors.initials: JJ
authors.lastname: York
authors.forename: J G
authors.initials: JG
authors.lastname: Young
authors.forename: K J
authors.initials: KJ
authors.lastname: Fahey