[Biochemical control of callus maturation--an experimental animal study].
OBJECTIVE
The purpose of this study was to verify the possibility of monitoring the callus maturation biochemically.


METHODS
In an experimental study on sheep, animals with bone fractures with a length 5 cm each were divided into group A (with concomitant damage of the soft tissue) and group B (without soft tissue damage). During callus traction and maturation, biochemical parameters (osteocalcin, AlP, NTx, calcium, phosphate) were measured. Animals of each group (A+B) were euthanised at fixed points in time (according to protocol) and the callus was prepared histologically.


RESULTS
Total AlP and NTx values were initially reduced during the callus traction and maturation, but increased significantly at the time of Docking+1 transportation time. At the same time, the histological evaluation showed a definite increase of the bone stabilisation, which approximately resembles the structure of a healthy bone as reference.


CONCLUSIONS
With the combination of the AlP and NTx values already determined in the daily clinical routine the monitoring of callus maturation in animal studies becomes possible.
Schmidt
J
J
Unfallchirurgie, HELIOS Klinikum Berlin-Buch. joerg.schmidt@helios-kliniken.de
Winker
H
H
Schmidt
I
I
Ekkernkamp
A
A
Merk
H
H
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D015519:Bone Density / Q000502:physiology; D001861:Bone Regeneration / Q000502:physiology; D001842:Bone and Bones / Q000473:pathology / Q000503:physiopathology; D002146:Bony Callus / Q000473:pathology / Q000503:physiopathology*; D002118:Calcium / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism*; D017102:Fracture Healing / Q000502:physiology*; D050723:Fractures, Bone / Q000473:pathology / Q000503:physiopathology*; D015675:Osteocalcin / Q000378:metabolism*; D019857:Osteogenesis, Distraction; D010455:Peptides / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D012756:Sheep; D017695:Soft Tissue Injuries / Q000473:pathology / Q000503:physiopathology*
D024042:Collagen Type I; D010455:Peptides; D010710:Phosphates; C052929:collagen type I trimeric cross-linked peptide; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D002118:Calcium