Chemoenzymatic acylation of aminoglycoside antibiotics.
The chemoenzymatic installation of the clinically valuable (S)-4-amino-2-hydroxybutyryl side chain onto a number of 2-deoxystreptamine-containing aminoglycosides is described using the purified Bacillus circulans biosynthetic enzymes BtrH and BtrG in combination with a synthetic acyl-SNAC surrogate substrate.
Llewellyn
Nicholas M
NM
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK. nml30@cam.ac.uk
Spencer
Jonathan B
JB
D000215:Acylation; D000617:Aminoglycosides / Q000138:chemical synthesis*; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis*; D001426:Bacterial Proteins / Q000378:metabolism; D002076:Butirosin Sulfate / Q000138:chemical synthesis / Q000494:pharmacology; D007202:Indicators and Reagents; D026901:Membrane Transport Proteins / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C089405:ButB protein, Bacillus circulans; D007202:Indicators and Reagents; D026901:Membrane Transport Proteins; D002076:Butirosin Sulfate; C513572:BtrR aminotransferase, Bacillus circulans; D000637:Transaminases