title: Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis.
abstract: A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis. The complete nucleotide sequences of genomic segments S2-S5 and S7-S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5' (AGUUU......UUUGUGC) 3' were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus within the family Reoviridae. Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana.
authors.affiliation: Population Ecology Group, Faculty of Forestry and Environmental Management, University of New Brunswick, Fredericton, NB, Canada E3B 6C2.
authors.forename: Robert I
authors.initials: RI
authors.lastname: Graham
authors.forename: Benoit
authors.initials: B
authors.lastname: Morin
authors.forename: Renée
authors.initials: R
authors.lastname: Lapointe
authors.forename: Vincent G
authors.initials: VG
authors.lastname: Nealis
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Lucarotti
mesh_terms: D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012087:Reoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase