Conformational analysis of a covalently cross-linked Watson-Crick base pair model.
Low-temperature NMR experiments and molecular modeling have been used to characterize the conformational behavior of a covalently cross-linked DNA base pair model. The data suggest that Watson-Crick or reverse Watson-Crick hydrogen bonding geometries have similar energies and can interconvert at low temperatures. This low-temperature process involves rotation about the crosslink CH(2)C(5') (psi) carbon-carbon bond, which is energetically preferred over the alternate CH(2)N(3) (phi) carbon-nitrogen bond rotation.
Jensen
Erik A
EA
Department of Chemistry, Pomona College, 645 North College Avenue, Claremont, CA 91711, USA.
Allen
Benjamin D
BD
Kishi
Yoshito
Y
O'Leary
Daniel J
DJ
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D013696:Temperature
D004247:DNA