title: Nanoimmunoliposome delivery of superparamagnetic iron oxide markedly enhances targeting and uptake in human cancer cells in vitro and in vivo.
abstract: To circumvent the problem of reduction of the supermagnetic properties of superparamagnetic iron oxide (SPIO) nanoparticles after chemical modification to conjugate targeting molecules, we have adapted a tumor-targeting nanoimmunoliposome platform technology (scL) to encapsulate and deliver SPIO (scL-SPIO) in vitro and in vivo without chemical modification. Scanning probe microscopy, confocal microscopy, and Prussian blue staining were used to analyze the scL-SPIO and assess intracellular uptake and distribution of SPIO in vitro. In vivo targeting and tumor-specific uptake of scL-SPIO was examined using fluorescent-labeled SPIO. We demonstrated that SPIO encapsulation in the scL complex results in an approximately 11-fold increase in SPIO uptake in human cancer cells in vitro, with distribution to cytoplasm and nucleus. Moreover, the scL nanocomplex specifically and efficiently delivered SPIO into tumor cells after systemic administration, demonstrating the potential of this approach to enhance local tumor concentration and the utility of SPIO for clinical applications.
authors.affiliation: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA.
authors.forename: Chengli
authors.initials: C
authors.lastname: Yang
authors.forename: Antonina
authors.initials: A
authors.lastname: Rait
authors.forename: Kathleen F
authors.initials: KF
authors.lastname: Pirollo
authors.forename: John A
authors.initials: JA
authors.lastname: Dagata
authors.forename: Natalia
authors.initials: N
authors.lastname: Farkas
authors.forename: Esther H
authors.initials: EH
authors.lastname: Chang
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D005290:Ferric Compounds / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D008081:Liposomes / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods; D018613:Microscopy, Confocal; D020527:Microscopy, Scanning Probe; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005290:Ferric Compounds; D008081:Liposomes; C000499:ferric oxide