Cogniauxia podolaena: bioassay-guided fractionation of defoliated stems, isolation of active compounds, antiplasmodial activity and cytotoxicity.
COGNIAUXIA PODOLAENA Baill. (Cucurbitaceae) is traditionally used in Congo Brazzaville for the treatment of malaria. We assessed the antiplasmodial activity of the plant and isolated some of the compounds responsible for this activity. It was the first time that a chemical study of this plant has been undertaken. Three triterpenes were isolated: cucurbitacin B ( 1), cucurbitacin D ( 2) and 20-epibryonolic acid ( 3) and their structures were assigned from spectroscopic evidence and comparison with published data. The crystallographic structure of 3 was determined. All fractions and compounds obtained in this study were assayed for antiplasmodial activity (on FcM29, a chloroquine-resistant strain of P. FALCIPARUM) and cytotoxicity (on KB and Vero cell lines). The IC50 values of 1, 2 and 3 are 1.6, 4 and 2 microg/mL on FcM29. Both 1 and 2 have a high cytotoxicity whereas 3 shows a better selectivity index.
Banzouzi
Jean Théophile
JT
Institut de Chimie des Substances Naturelles (ICSN-CNRS), Gif-sur-Yvette Cedex, France. jean-theophile.banzouzi@icsn.cnrs-gif.fr
Soh
Patrice Njomnang
PN
Mbatchi
Bernard
B
Cavé
Adrien
A
Ramos
Suzanne
S
Retailleau
Pascal
P
Rakotonandrasana
Olga
O
Berry
Antoine
A
Benoit-Vical
Françoise
F
D000818:Animals; D000962:Antimalarials / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005591:Chemical Fractionation; D002522:Chlorocebus aethiops; D002851:Chromatography, High Pressure Liquid; D018360:Crystallography, X-Ray; D019662:Cucurbitaceae / Q000737:chemistry*; D006801:Humans; D007624:KB Cells; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects; D018675:Toxicity Tests; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014709:Vero Cells
C533643:20-epibryonolic acid; D000962:Antimalarials; D010936:Plant Extracts; D014315:Triterpenes; C041246:cucurbitacin B; C038105:cucurbitacin D