title: Oxidative stress increases levels of endogenous amyloid-beta peptides secreted from primary chick brain neurons.
abstract: Oxidative damage is associated with Alzheimer's disease and mild cognitive impairment, but its relationship to the development of neuropathological lesions involving accumulation of amyloid-beta (Abeta) peptides and hyperphosphorylated tau protein remains poorly understood. We show that inducing oxidative stress in primary chick brain neurons by exposure to sublethal doses of H(2)O(2 )increases levels of total secreted endogenous Abeta by 2.4-fold after 20 h. This occurs in the absence of changes to intracellular amyloid precursor protein or tau protein levels, while heat-shock protein 90 is elevated 2.5-fold. These results are consistent with the hypothesis that aging-associated oxidative stress contributes to increasing Abeta generation and up-regulation of molecular chaperones in Alzheimer's disease.
authors.affiliation: Brain and Mind Research Institute, University of Sydney, Camperdown, NSW, Australia. cgoldsbury@usyd.edu.au
authors.forename: Claire
authors.initials: C
authors.lastname: Goldsbury
authors.forename: Ineka T
authors.initials: IT
authors.lastname: Whiteman
authors.forename: Erica V
authors.initials: EV
authors.lastname: Jeong
authors.forename: Yun-An
authors.initials: YA
authors.lastname: Lim
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D009474:Neurons / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*
chemical_list: D016229:Amyloid beta-Peptides