title: Expression, purification, crystallization and preliminary X-ray analysis of an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.
abstract: Helicobacter pylori is a dangerous human pathogen that resides in the upper gastrointestinal tract. Little is known about its metabolism and with the onset of antibiotic resistance new treatments are required. In this study, the expression, purification, crystallization and preliminary X-ray diffraction of an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from H. pylori are reported.
authors.affiliation: Henry Wellcome Laboratories for Structural Biology, University of Leicester, Leicester LE19HN, England.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Elliott
authors.forename: Shabaz
authors.initials: S
authors.lastname: Mohammad
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Melrose
authors.forename: Peter C E
authors.initials: PC
authors.lastname: Moody
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016480:Helicobacter pylori / Q000201:enzymology*; D009243:NAD / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D017931:DNA Primers; D009243:NAD; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases