[Comparative assessment of soil microbial biomass determined by the methods of direct microscopy and substrate-induced respiration].
The content of microbial biomass (MB) was determined in samples of gray forest, chestnut, and tundra soils with different physicochemical properties (0.4-22.7% Corg; 8.4-26.8% silt particles; pH 4.3-8.4) by the methods of substrate-induced respiration (MB(SIR)) and direct microscopy (MB(M)). The samples of two upper soil layers, 0-5 and 5-10 cm (without plant litter), from different ecosystems (forest, forest shelter belt, meadow, fallow, and arable land) and elements of relief of interfluvial tundra (block/upper land plateau, depression between blocks) have been analyzed. The content of microbial biomass in the 0-5-cm soil layer was 216-8134 and 348-7513 microg C/g soil as measured by the methods of substrate-induced respiration and direct microscopy, respectively. The MB(SIR) and MB(M) values closely correlated with each other: r = 0.90 and 0.74 for 0-5 and 5-10 cm, respectively. The average MB(SIR)/MB(M) ratio was 90 and 60% for 0-5 and 5-10 cm, respectively. The portion of microbial carbon in total organic soil carbon was, on average, 4 and 3% (SIR) and 5 and 7% (direct microscopy) for 0-5 and 5-10 cm, respectively. Possible reasons for the differences between MB(SIR) and MB(M) values in the soils under study are discussed.
Anan'eva
N D
ND
Polianskaia
L M
LM
Sus'ian
E A
EA
Vasenkina
I V
IV
Wirt
S
S
Zviagintsev
D G
DG
D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D018533:Biomass; D002244:Carbon / Q000032:analysis / Q000378:metabolism; D019069:Cell Respiration; D015169:Colony Count, Microbial; D005658:Fungi / Q000302:isolation & purification* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008853:Microscopy; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology
D012987:Soil; D002244:Carbon; D005947:Glucose