[Effects of cellobiose lipid B on Saccharomyces cerevisiae cells: K+ leakage and inhibition of polyphosphate accumulation].
Cellobiose lipid B, a natural fungicide produced by the yeast Pseudozyma fusiformata, induces the leakage of K+ and ATP from cells of Saccharomyces cerevisiae. The presence of glucose decreases the effective concentration of cellobiose lipid B. The concentration of cellobiose lipid B was selected that results in a high rate of K+ leakage and a five- to sevenfold decrease in the intracellular ATP content, while the accumulation of acid-soluble polyphosphates decreased only by half. These results indicate the possibility of synthesis of these polymers independently of the ATP level and of the ion gradient on the plasma membrane.
Kulakovskaia
E V
EV
Ivanov
A Iu
AIu
Kulakovskaia
T V
TV
Vagabov
V M
VM
Kulaev
I S
IS
D000255:Adenosine Triphosphate / Q000378:metabolism; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D001487:Basidiomycota / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006017:Glycolipids / Q000378:metabolism / Q000494:pharmacology*; D011122:Polyphosphates / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism
D000935:Antifungal Agents; D006017:Glycolipids; D011122:Polyphosphates; D000255:Adenosine Triphosphate; D011188:Potassium