Novel egg white-based 3-D cell culture system.
Although three dimensional (3-D) cell culture systems have numerous advantages over traditional monolayer culture, the currently available 3-D cell culture media are cost-prohibitive for regular use by the majority of research laboratories. Here we show a simple system based on avian egg white that supports growth of cells in 3-D, at a significantly decreased cost. Specifically, we show that growth of immortalized human breast epithelial cells (MCF10A) in egg white-based medium results in formation of acini with hollow lumens, apoptotic clearance of the cells in the lumen, and apicobasal polarization comparable to what has been described using established 3-D culture media such as reconstituted basement membrane preparations (BM). There was no significant difference in MCF10A proliferation and acinar size between egg white and BM. We also cultured different established cell lines, oncogene-transformed MCF10A, and mouse mammary epithelial cells in egg white and BM, and observed similar morphology. In summary, our data convincingly argue that egg white can be used as a suitable alternative model for 3-D cell culture studies. We strongly believe that this simple and inexpensive method should allow researchers to perform 3-D cell culture experiments on a regular basis, and result in a dramatic increase of use of the 3-D cell culture in research. Thus, this finding lays the foundation for significantly increased, cost-effective use of 3-D cultures in cell biology.
Kaipparettu
Benny Abraham
BA
Department of Medicine, Lester and Sue Smith Breast Center, Baylor College of Medicine, Houston, TX 77030, USA.
Kuiatse
Isere
I
Tak-Yee Chan
Bonita
B
Benny Kaipparettu
Meju
M
Lee
Adrian V
AV
Oesterreich
Steffi
S
D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured / Q000166:cytology*; D003094:Collagen; D004338:Drug Combinations; D004527:Egg Proteins / Q000494:pharmacology; D004529:Egg White; D006801:Humans; D007797:Laminin; D011509:Proteoglycans
D004338:Drug Combinations; D004527:Egg Proteins; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen