title: A viral suppressor P1/HC-pro increases the GFP gene expression in agrobacterium-mediated transient assay.
abstract: More than 20 post-transcriptional gene silencing (PTGS) suppressors have been found since HC-Pro, the first gene silencing suppressor, was found in 1998. The silencing suppressor strongly suggested that gene silencing functions as natural defense mechanisms against viruses. It also represented a valuable tool for the dissection of the gene silencing pathway. We have used P1/HC-Pro RNA silencing suppressor activity to increase green fluorescent protein (GFP) expression in tobacco using an Agrobacterium-mediated transient expression system. P1/HC-Pro stimulated GFP-gene expression but not dsGFP-gene expression was shown by RT-PCR, Northern and Western blot analysis. Expression of the gene silencing suppressor and the target gene provided a new strategy of heterogeneous gene expressing in plants. It may be of commercial significance to produce foreign proteins using plant bioreactors.
authors.affiliation: Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024 China.
authors.forename: Pengda
authors.initials: P
authors.lastname: Ma
authors.forename: Jinying
authors.initials: J
authors.lastname: Liu
authors.forename: Hongxia
authors.initials: H
authors.lastname: He
authors.forename: Meiying
authors.initials: M
authors.lastname: Yang
authors.forename: Meina
authors.initials: M
authors.lastname: Li
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhu
authors.forename: Xingzhi
authors.initials: X
authors.lastname: Wang
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus