Relationship between genomic types of Escherichia coli and clinical diseases.
In this study, by analysis of genome structures of E. coli, the relationships between the genomic types of E. coli and the associated diseases were investigated. Samples of sputum, urine and other excretions from patients with different infective diseases were collected. And 62 E. coli strains were isolated from these samples. Intact bacterial genomic DNA was cleaved with I-CeuI, separated by pulsed field gel electrophoresis and then typed on the basis of cleavage map. The results showed that 7 I-CeuI sites were found in all the genome structures of the 62 E. coli, indicating that there were 7 rrn operons in the genomes. The size of genome ranged from 4,500 kb to 5,000 kb. According to the genome structures, 62 E. coli strains were divided into 30 genome types. It was concluded that genome structures of E. coli isolated from the patients with different infective diseases varied to some extent, suggesting that some genome types of E. coli were closely related to some infective diseases.
Yi
Meiying
M
China-Japan Friendship Hospital, Beijing 100029, China.
Liu
Ruen
R
Huang
Hanju
H
D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D014644:Genetic Variation; D016680:Genome, Bacterial; D006801:Humans
D004269:DNA, Bacterial; D004706:Endodeoxyribonucleases; C071132:endodeoxyribonuclease I-CeuI