title: Induction of allergic contact dermatitis by astigmatid mite-derived monoterpene, alpha-acaridial.
abstract: alpha-Acaridial [2(E)-(4-methyl-3-pentenyl)butenedial] is a novel monoterpene secreted from the house dust mites. Because of its molecular nature of a highly reactive, small lipidic compound, we addressed whether alpha-acaridial might function as a haptenic allergen that induced allergic contact dermatitis. Mice sensitized with alpha-acaridial were challenged by the same antigen on the ear skin. After 2 days, significant ear swelling with a prominent infiltration of CD4(+) T lymphocytes was observed. In vitro, alpha-acaridial exhibited an outstanding ability to quickly interact with and chemically modify a reference protein. Virtually all cysteine residues and a sizable fraction of lysine residues were found to be selectively modified, suggesting that alpha-acaridial could potentially interact with any proteins. Previously, numerous mite-derived proteinaceous allergens have been associated with contact dermatitis. Our study now emphasizes that small lipidic compounds released from mites comprise a new class of mite allergens, and therefore, is of significant medical implications.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Sasai
authors.forename: Yunosuke
authors.initials: Y
authors.lastname: Hirano
authors.forename: Sayaka
authors.initials: S
authors.lastname: Maeda
authors.forename: Isamu
authors.initials: I
authors.lastname: Matsunaga
authors.forename: Atsushi
authors.initials: A
authors.lastname: Otsuka
authors.forename: Daisuke
authors.initials: D
authors.lastname: Morita
authors.forename: Ritsuo
authors.initials: R
authors.lastname: Nishida
authors.forename: Hideo
authors.initials: H
authors.lastname: Nakayama
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Kuwahara
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sugita
authors.forename: Naoki
authors.initials: N
authors.lastname: Mori
mesh_terms: D000447:Aldehydes / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000737:chemistry / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D039821:Monoterpenes / Q000737:chemistry / Q000276:immunology*
chemical_list: D000447:Aldehydes; D039741:Antigens, Dermatophagoides; D039821:Monoterpenes; C448740:beta-acaridial