The triple helix: 50 years later, the outcome.
Triplex-forming oligonucleotides constitute an interesting DNA sequence-specific tool that can be used to target cleaving or cross-linking agents, transcription factors or nucleases to a chosen site on the DNA. They are not only used as biotechnological tools but also to induce modifications on DNA with the aim to control gene expression, such as by site-directed mutagenesis or DNA recombination. Here, we report the state of art of the triplex-based anti-gene strategy 50 years after the discovery of such a structure, and we show the importance of the actual applications and the main challenges that we still have ahead of us.
Duca
Maria
M
LCMBA CNRS UMR6001, University of Nice-Sophia Antipolis, Parc Valrose, 06108 NICE Cedex 2, France.
Vekhoff
Pierre
P
Oussedik
Kahina
K
Halby
Ludovic
L
Arimondo
Paola B
PB
D000818:Animals; D001709:Biotechnology; D004247:DNA / Q000737:chemistry* / Q000266:history; D005786:Gene Expression Regulation; D049673:History, 20th Century; D006801:Humans; D051379:Mice; D009841:Oligonucleotides / Q000737:chemistry
D009841:Oligonucleotides; C051802:triplex DNA; D004247:DNA