title: Vaccination against Marek's disease reduces telomerase activity and viral gene transcription in peripheral blood leukocytes from challenged chickens.
abstract: We investigated whether telomerase activity and viral gene transcription were associated with protection against the RB-1B strain of Marek's disease virus (MDV) in chickens vaccinated with Rispens CVI988 or the herpes virus of turkey (HVT). Telomerase activity in peripheral blood leukocytes (PBLs) seemed to be an appropriate marker of lymphoma and levels of viral transcription were correlated with the virulence of MDV strains. Vaccinated protected birds had lower levels of telomerase activity and RB-1B viral gene transcription than unvaccinated chickens infected with RB-1B. The decrease in RB-1B viral transcription was more marked in chickens vaccinated with CVI988 than in those vaccinated with HVT. Indeed, RB-1B viral transcription was not detectable after 14 days post-challenge. In conclusion, telomerase activity and gene transcription in challenge MDV strains are potential new reliable criteria of protection in vaccinated chickens.
authors.affiliation: Equipe Télomérase et Lymphome Viro-Induit Unité IASP1282, INRA, F-37380 Nouzilly, France.
authors.forename: Manel
authors.initials: M
authors.lastname: Debba-Pavard
authors.forename: Aouatef
authors.initials: A
authors.lastname: Ait-Lounis
authors.forename: Denis
authors.initials: D
authors.lastname: Soubieux
authors.forename: Denis
authors.initials: D
authors.lastname: Rasschaert
authors.forename: Ginette
authors.initials: G
authors.lastname: Dambrine
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000276:immunology; D007962:Leukocytes / Q000201:enzymology / Q000276:immunology / Q000821:virology*; D008223:Lymphoma / Q000201:enzymology / Q000821:virology; D025922:Mardivirus / Q000235:genetics* / Q000276:immunology; D008380:Marek Disease / Q000201:enzymology / Q000276:immunology* / Q000517:prevention & control / Q000821:virology*; D022501:Marek Disease Vaccines / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D022501:Marek Disease Vaccines; D019098:Telomerase