A comparison of the physical and chemical differences between cancellous and cortical bovine bone mineral at two ages.
To assess possible differences between the mineral phases of cortical and cancellous bone, the structure and composition of isolated bovine mineral crystals from young (1-3 months) and old (4-5 years) postnatal bovine animals were analyzed by a variety of complementary techniques: chemical analyses, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and (31)P solid-state magic angle spinning nuclear magnetic resonance spectroscopy (NMR). This combination of methods represents the most complete physicochemical characterization of cancellous and cortical bone mineral completed thus far. Spectra obtained from XRD, FTIR, and (31)P NMR all confirmed that the mineral was calcium phosphate in the form of carbonated apatite; however, a crystal maturation process was evident between the young and old and between cancellous and cortical mineral crystals. Two-way analyses of variance showed larger increases of crystal size and Ca/P ratio for the cortical vs. cancellous bone of 1-3 month than the 4-5 year animals. The Ca/(P + CO(3)) remained nearly constant within a given bone type and in both bone types at 4-5 years. The carbonate and phosphate FTIR band ratios revealed a decrease of labile ions with age and in cortical, relative to cancellous, bone. Overall, the same aging or maturation trends were observed for young vs. old and cancellous vs. cortical. Based on the larger proportion of newly formed bone in cancellous bone relative to cortical bone, the major differences between the cancellous and cortical mineral crystals must be ascribed to differences in average age of the crystals.
Kuhn
Liisa T
LT
Department of Orthopedic Surgery, Children's Hospital, Harvard Medical School, Boston, MA, USA. lkuhn@uchc.edu
Grynpas
Marc D
MD
Rey
Christian C
CC
Wu
Yaotang
Y
Ackerman
Jerome L
JL
Glimcher
Melvin J
MJ
D000375:Aging / Q000502:physiology*; D000818:Animals; D001031:Apatites / Q000032:analysis; D015519:Bone Density; D002113:Calcification, Physiologic; D002130:Calcium Phosphates / Q000032:analysis; D002417:Cattle; D003460:Crystallization; D005360:Fibula / Q000737:chemistry / Q000000981:diagnostic imaging / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011859:Radiography; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013977:Tibia / Q000737:chemistry / Q000000981:diagnostic imaging / Q000378:metabolism*; D014961:X-Ray Diffraction / Q000379:methods
D001031:Apatites; D002130:Calcium Phosphates; C020243:calcium phosphate