Antioxidant and anticancer efficacy of hesperidin in benzo(a)pyrene induced lung carcinogenesis in mice.
Chemoprevention is regarded as one of the most promising and realistic approaches in the prevention of cancer. Several bioactive compounds present in fruits and vegetables have revealed their cancer curative potential on lung cancer. Hesperidin is one such naturally occurring flavonoid widely found in citrus fruits. The aim of the present study is to divulge the chemopreventive nature of hesperidin during benzo(a)pyrene (B(a)P) induced lung cancer in Swiss albino mice. Administration of B(a)P (50 mg/kg body weight) to mice resulted in increased lipid peroxides (LPO), lung specific tumor marker carcinoembryonic antigen (CEA) and serum marker enzymes aryl hydrocarbon hydroxylase (AHH), gamma glutamyl transpeptidase (GGT), 5'nucleotidase (5'ND) and lactate dehydrogenase (LDH) with concomitant decrease in the levels of tissue antioxidants like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), vitamin E and vitamin C. Hesperidin supplementation (25 mg/kg body weight) significantly attenuated these alterations thereby showing potent anticancer effect in lung cancer. Further the antiproliferative effect of hesperidin was confirmed by histopathological analysis and proliferating cell nuclear antigen (PCNA) immunostaining. Overall, these findings substantiate the chemopreventive potential of hesperidin against chemically induced lung cancer in mice.
Kamaraj
Sattu
S
Department of Biochemistry, University of Madras, Guindy Campus, Chennai, 600 025, India.
Ramakrishnan
Gopalakrishnan
G
Anandakumar
Pandi
P
Jagan
Sundaram
S
Devaki
Thiruvengadam
T
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001564:Benzo(a)pyrene; D001835:Body Weight / Q000187:drug effects; D002272:Carcinoembryonic Antigen / Q000097:blood; D004354:Drug Screening Assays, Antitumor; D006569:Hesperidin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D016896:Treatment Outcome
D000970:Antineoplastic Agents; D000975:Antioxidants; D002272:Carcinoembryonic Antigen; D018809:Proliferating Cell Nuclear Antigen; D001564:Benzo(a)pyrene; D006569:Hesperidin