An extension of the Minute Virus of Mice tissue tropism.
Well-defined tissue tropism makes Autonomous Parvoviruses a valuable model for studies of virus-cell interactions and gene therapy research. We developed a new Minute Virus of Mice variant, different from the known prototype (MVMp) and immunosuppressive (MVMi) strains. The new virus variant, designated F1, was isolated from the culture of semi-permissive Fisher Rat Fibroblasts, F111, infected with MVMp. The F1 genome carried point mutations in regions known to determine the mutually restricted host ranges of MVMp and MVMi. In F111 cells, F1 cytotoxicity, gene expression and multiplication were significantly higher compared to MVMp. Conversely the wild-type virus propagated in MVMp-permissive cells more efficiently than the F1. Reversion of the F1-specific mutations to wild-type MVMp sequence, following reverse-passaging of the mutant virus in MVMp-permissive cells, confirmed a specific adaptation of the F1 virus to F111 cells. Considerable divergence in tissue specificities between the wild-type and mutant viruses was demonstrated in vivo.
Etingov
Igor
I
Department of Virology and Developmental Molecular Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel. etingov@bgu.ac.il
Itah
Refael
R
Mincberg
Michal
M
Keren-Naus
Ayelet
A
Nam
Hyun-Joo
HJ
Agbandje-McKenna
Mavis
M
Davis
Claytus
C
D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D014644:Genetic Variation; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D051379:Mice; D008915:Minute Virus of Mice / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D008958:Models, Molecular; D009928:Organ Specificity; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D051381:Rats; D014774:Virulence / Q000235:genetics
D036022:Capsid Proteins; D017931:DNA Primers; D004279:DNA, Viral; C442549:VP1 protein, Mice minute virus