Allopurinol, an inhibitor of purine catabolism, enhances susceptibility of tobacco to Tobacco mosaic virus.
Tobacco (cv. Xanthi nn) plants were watered with allopurinol [4-hydroxypyrazolo (3,4-d) pyrimidine, HPP], a xanthine oxidoreductase (XOR) inhibitor, to investigate its effects on infection by Tobacco mosaic virus engineered to express the green fluorescent protein (TMV.GFP). TMV.GFP infection was monitored by examination of inoculated leaves under UV light, by confocal scanning laser microscopy and by epifluorescence microscopy. Susceptibility to TMV.GFP was enhanced in HPP-treated plants. This was seen as a statistically significant increase in numbers of infection sites per leaf and in the number of infected cells per infection site. Two hypotheses are discussed to explain the enhanced susceptibility. The inhibition exerted by HPP against XOR activity could provoke either (i) an increased adenine and guanine nucleotide pool, which could facilitate viral RNA synthesis or (ii) it could cause changes in IAA/auxin levels, which has been proposed to influence TMV susceptibility in tobacco.
Silvestri
Simona
S
Dipartimento di Scienze Agrarie e Ambientali, Sezione di Arboricoltura e Protezione delle Piante, Università degli Studi di Perugia, Via Borgo XX Giugno 74, I-06121 Perugia, Italy.
Murphy
Alex M
AM
Buonaurio
Roberto
R
Carr
John P
JP
D000493:Allopurinol / Q000494:pharmacology*; D004198:Disease Susceptibility; D004791:Enzyme Inhibitors / Q000494:pharmacology; D010935:Plant Diseases / Q000821:virology*; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology / Q000502:physiology / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000187:drug effects* / Q000472:pathogenicity; D014968:Xanthine Dehydrogenase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
D004791:Enzyme Inhibitors; D010940:Plant Proteins; D011687:Purines; D000493:Allopurinol; D014968:Xanthine Dehydrogenase; C030985:purine