Beta1,2-xylosyltransferase Cxt1p is solely responsible for xylose incorporation into Cryptococcus neoformans glycosphingolipids.
The Manalpha1,3(Xylbeta1,2)Manalpha structural motif is common to both capsular polysaccharides of Cryptococcus neoformans and to cryptococcal glycosphingolipids. Comparative analysis of glycosphingolipid structural profiles in wild-type and mutant strains showed that the Xylbeta1,2-transferase (Cxt1p) that participates in capsular polysaccharide biosynthesis is also the sole transferase responsible for adding xylose to C. neoformans glycosphingolipids.
Castle
Sherry A
SA
Department of Chemistry, University of New Hampshire, G229 Parsons Hall, Durham, New Hampshire 03824-3598, USA.
Owuor
Elizabeth A
EA
Thompson
Stephanie H
SH
Garnsey
Michelle R
MR
Klutts
J Stacey
JS
Doering
Tamara L
TL
Levery
Steven B
SB
D003455:Cryptococcus neoformans / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006028:Glycosphingolipids / Q000737:chemistry / Q000378:metabolism*; D010430:Pentosyltransferases / Q000235:genetics / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
D005656:Fungal Proteins; D006028:Glycosphingolipids; D014994:Xylose; D010430:Pentosyltransferases; C408586:beta 1,2-xylosyltransferase