title: [Effect of boron and fluoride on the expression of enamelin in rat incisor].
abstract: OBJECTIVE
  To investigate the effects of overdose fluoride, boron and two factors on the expression of enamelin in rat incisor.
  
  
  METHODS
  32 Wistar rats were randomly divided into 4 groups. Group I: The distilled water was given. Group II: 220 mg/L NaF were given. Group III: 382 mg/L Na2B4O2.10H2O were given. Group IV: 220 mg/L NaF and 382 mg/L Na2B4O2.10H2O were given. The rats were sacrificed in the eighth week. HE staining was used to observe the morphology of ameloblasts. Immunohistochemical staining was used for study the expression of enamelin in rat incisors.
  
  
  RESULTS
  The results showed that the expression of enamelin was reduced in the group II (P<0.01). Compared with group I, the expression of enamelin in group IV had no significant difference. The expression of enamelin in group IV and group II had significant difference (P<0.01).
  
  
  CONCLUSIONS
  The overdose fluoride can inhibit the expression of enamelin. The effection was weaken when boron added. Boron reduced the toxicity of fluoride on teeth.
authors.affiliation: Department of Operative Dentistry and Endodontics, School of Stomatology, Tongji University, Shanghai 200072, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Wu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Chun-Na
authors.initials: CN
authors.lastname: Gao
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Wei
mesh_terms: D000565:Ameloblasts; D000818:Animals; D001895:Boron; D003746:Dental Enamel Proteins; D005459:Fluorides; D007180:Incisor; D010710:Phosphates; D051381:Rats; D017208:Rats, Wistar; D014070:Tooth
chemical_list: D003746:Dental Enamel Proteins; D010710:Phosphates; C038456:tuftelin; C012980:fluorophosphate; D001895:Boron; D005459:Fluorides