title: Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants.
abstract: Snakin-1 (SN1), a cysteine-rich peptide with broad-spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highly evolutionary conservation with 91-98% identity values. Potato plants (S. tuberosum subsp. tuberosum cv. Kennebec) were transformed via Agrobacterium tumefaciens with a construct encoding the S. chacoense SN1 gene under the regulation of the ubiquitous CaMV 35S promoter. Transgenic lines were molecularly characterized and challenged with either Rhizoctonia solani or Erwinia carotovora to analyse whether constitutive in vivo overexpression of the SN1 gene may lead to disease resistance. Only transgenic lines that accumulated high levels of SN1 mRNA exhibited significant symptom reductions of R. solani infection such as stem cankers and damping-off. Furthermore, these overexpressing lines showed significantly higher survival rates throughout the fungal resistance bioassays. In addition, the same lines showed significant protection against E. carotovora measured as: a reduction of lesion areas (from 46.5 to 88.1% with respect to the wild-type), number of fallen leaves and thickened or necrotic stems. Enhanced resistance to these two important potato pathogens suggests in vivo antifungal and antibacterial activity of SN1 and thus its possible biotechnological application.
authors.affiliation: Instituto de Biotecnología, CNIA, Instituto Nacional de Tecnología Agropecuaria-Castelar, Los Reseros y Las Cabañas, B1712WAA Hurlingham, Provincia de Buenos Aires, Argentina.
authors.forename: Natalia I
authors.initials: NI
authors.lastname: Almasia
authors.forename: Ariel A
authors.initials: AA
authors.lastname: Bazzini
authors.forename: H Esteban
authors.initials: HE
authors.lastname: Hopp
authors.forename: Cecilia
authors.initials: C
authors.lastname: Vazquez-Rovere
mesh_terms: D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D016973:Pectobacterium carotovorum / Q000254:growth & development*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000382:microbiology; D012232:Rhizoctonia / Q000254:growth & development*; D011198:Solanum tuberosum / Q000235:genetics* / Q000382:microbiology*
chemical_list: D010940:Plant Proteins; C117071:SN1 protein, Solanum tuberosum