Saccharomyces cerevisiae Rds2 transcription factor involvement in cell wall composition and architecture.
Although the cell wall is very important in yeasts, relatively little is known about the relationship between its structure and function. In Saccharomyces cerevisiae, a family of 55 transcription factor proteins unique to fungi, so-called zinc cluster proteins, has been described. Of these, Rds2 has been identified as an activator/inhibitor of gluconeogenesis. However, previous studies have pointed out additional roles for this protein, specifically, in the modulation of cell-wall architecture and drug sensitivity. In this work, evidence regarding the role of Rds2 as a regulator of cell-wall architecture and composition is presented based on phenotypical analysis of the cell walls prepared from a S. cerevisiae Rds2 mutant strain. Analyses of the sensitivity of this rds2Delta mutant to different drugs and to osmotic stress showed that Rds2 is indeed involved in the drug-sensitivity response and plays a role in determining osmotic sensitivity.
Moreno
Inmaculada
I
Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Valencia, Spain.
Tutrone
Nunzia
N
Sentandreu
Rafael
R
Valentín
Eulogio
E
D000935:Antifungal Agents / Q000494:pharmacology*; D001557:Benzenesulfonates / Q000494:pharmacology; D002473:Cell Wall / Q000737:chemistry* / Q000187:drug effects*; D005456:Fluorescent Dyes / Q000494:pharmacology; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000187:drug effects*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
D000935:Antifungal Agents; D001557:Benzenesulfonates; D005456:Fluorescent Dyes; C527549:Rds2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C007061:C.I. Fluorescent Brightening Agent 28; D004693:Glucan Endo-1,3-beta-D-Glucosidase