Evaluation of methods for rapid detection of resistance to isoniazid and rifampin in Mycobacterium tuberculosis isolates collected in the Caribbean.
The rapid identification of drug-resistant strains of Mycobacterium tuberculosis is crucial for the timely initiation of appropriate antituberculosis therapy. The performance of the Genotype MTBDRplus assay was compared with that of the Bactec 460 TB system, a "gold standard" culture-based method. The Genotype MTBDRplus assay was quicker and more cost-effective for the detection of rifampin resistance, but it was not as good for the detection of isoniazid-resistant strains in our setting.
Akpaka
Patrick Eberechi
PE
Department of Para-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad & Tobago. Patrick.Akpaka@sta.uwi.edu
Baboolal
Shirematee
S
Clarke
Denise
D
Francis
Lorraine
L
Rastogi
Nalin
N
D000995:Antitubercular Agents / Q000494:pharmacology*; D017691:Caribbean Region; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000191:economics / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000191:economics / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D012293:Rifampin / Q000494:pharmacology*; D013997:Time Factors; D014376:Tuberculosis / Q000382:microbiology*
D000995:Antitubercular Agents; D004269:DNA, Bacterial; D007538:Isoniazid; D012293:Rifampin