Surface immobilization of biomolecules by click sulfonamide reaction.
Alkyne-modified biomolecules can be immobilized site- and chemoselectively on sulfonylazide slides under very mild conditions by means of the click sulfonamide reaction.
Govindaraju
Thimmaiah
T
Department of Chemical Biology, Max-Planck-Institut für Molekulare Physiologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany.
Jonkheijm
Pascal
P
Gogolin
Lars
L
Schroeder
Hendrik
H
Becker
Christian F W
CF
Niemeyer
Christof M
CM
Waldmann
Herbert
H
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D055598:Chemical Phenomena; D002621:Chemistry; D050091:Dendrimers; D005456:Fluorescent Dyes; D005898:Glass; D006801:Humans; D007202:Indicators and Reagents; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D011073:Polyamines; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D013449:Sulfonamides / Q000737:chemistry*; D013499:Surface Properties; D020691:rab GTP-Binding Proteins / Q000737:chemistry
D000480:Alkynes; D001386:Azides; D050091:Dendrimers; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; C104700:PAMAM Starburst; D010455:Peptides; D011073:Polyamines; D029701:Saccharomyces cerevisiae Proteins; D013449:Sulfonamides; C079005:YPT7 protein, S cerevisiae; D020691:rab GTP-Binding Proteins