ORF-C4 from the early branching eukaryote Giardia lamblia displays characteristics of alpha-crystallin small heat-shock proteins.
Giardia lamblia is a medically important protozoan parasite with a basal position in the eukaryotic lineage and is an interesting model to explain the evolution of biochemical events in eukaryotic cells. G. lamblia trophozoites undergo significant changes in order to survive outside the intestine of their host by differentiating into infective cysts. In the present study, we characterize the previously identified Orf-C4 (G. lamblia open reading frame C4) gene, which is considered to be specific to G. lamblia. It encodes a 22 kDa protein that assembles into high-molecular-mass complexes during the entire life cycle of the parasite. ORF-C4 localizes to the cytoplasm of trophozoites and cysts, and forms large spherical aggregates when overexpressed. ORF-C4 overexpression and down-regulation do not affect trophozoite viability; however, differentiation into cysts is slightly delayed when the expression of ORF-C4 is down-regulated. In addition, ORF-C4 protein expression is modified under specific stress-inducing conditions. Neither orthologous proteins nor conserved domains are found in databases by conventional sequence analysis of the predicted protein. However, ORF-C4 contains a region which is similar structurally to the alpha-crystallin domain of sHsps (small heat-shock proteins). In the present study, we show the potential role of ORF-C4 as a small chaperone which is involved in the response to stress (including encystation) in G. lamblia.
Nores
María J
MJ
School of Medicine, Catholic University of Córdoba, Jacinto Rios 571, CP5004, Cordoba, Argentina.
Prucca
César G
CG
Quiroga
Rodrigo
R
Elías
Eliana V
EV
Cavallín
Lucas
L
Price
Argenta M
AM
Saura
Alicia
A
Carranza
Pedro G
PG
Gottig
Natalia
N
Solari
Alberto J
AJ
Lujan
Hugo D
HD
D000818:Animals; D005786:Gene Expression Regulation; D016829:Giardia lamblia / Q000235:genetics / Q000502:physiology*; D050888:Heat-Shock Proteins, Small / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological; D038201:alpha-Crystallins / Q000235:genetics / Q000378:metabolism
D050888:Heat-Shock Proteins, Small; D015800:Protozoan Proteins; D038201:alpha-Crystallins