Pyridoxine 5'-phosphate oxidase, not pyridoxal kinase, involves in long-term potentiation induction in the rat dentate gyrus.
In this study, we investigated whether the expression of pyridoxal kinase (PLK) and pyridoxine-5'-phosphate oxidase (PNPO) are altered following long-term potentiation (LTP) induction, and whether Tat-PLK and Tat-PNPO transductions affect LTP induction and paired-pulse responses in the rat dentate gyrus (DG). PNPO immunoreactivity was markedly increased in dentate granule cells after the induction of LTP, but that of PLK was not. Tat-PNPO (20 and 200 microg/kg), but not Tat-PLK or vitamin B6 precursors, treatments, increased the efficiency of high frequency stimulus-induced potentiation of populations spike amplitude when compared with saline-, or Tat-protein-treated animals. These changes correlated with the alterations in PNPO activity and its immunoreactivity. In addition, Tat-PNPO transduction increased paired-pulse facilitation but had no effect on the fast and late paired-pulse inhibitions. These findings suggest that PNPO may play a role in activity-dependent regulation of PLP level in the brain, which is involved in LTP induction and paired-pulse facilitation but not in enhancement of GABAergic inhibition.
Kwak
Sung-Eun
SE
Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do 200-702, Republic of Korea.
Kim
Ji-Eun
JE
Kim
Dae-Won
DW
Kwon
Oh-Shin
OS
Choi
Soo-Young
SY
Kang
Tae-Cheon
TC
D000200:Action Potentials / Q000235:genetics; D000818:Animals; D018891:Dentate Gyrus / Q000201:enzymology*; D004558:Electric Stimulation; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D015696:Gene Products, tat / Q000235:genetics; D017774:Long-Term Potentiation / Q000235:genetics*; D009433:Neural Inhibition / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D011731:Pyridoxal Kinase / Q000378:metabolism*; D011734:Pyridoxaminephosphate Oxidase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014161:Transduction, Genetic / Q000379:methods; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D005680:gamma-Aminobutyric Acid; D011734:Pyridoxaminephosphate Oxidase; D011731:Pyridoxal Kinase