Methyl jasmonate decreases membrane fluidity and induces apoptosis through tumor necrosis factor receptor 1 in breast cancer cells.
In recent years, studies with plant compounds have shown both chemotherapeutic and chemopreventive properties. This study with plant stress hormones (jasmonates) showed growth inhibitory effects in breast cancer cells. cis-Jasmone and methyl jasmonate (MJ) inhibited the long-term proliferation of MDA-MB-435 and MCF-7 cells. Cell cycle analysis showed G0/G1 and S-phase arrest with increasing apoptotic population. Cellular signaling studies with MJ showed decreased membrane fluidity and activation of extrinsic and intrinsic apoptotic pathways. Specifically in extrinsic apoptotic pathway increased expression of TNF receptor 1, activation of mitogen-activated protein kinase and caspase-8 was observed. MJ also decreased the mitochondrial membrane potential and activated caspase-3 in breast cancer cells. In conclusion our results revealed novel-signaling mechanism of MJ in breast cancer cells, indicating that MJ could have potential applications for chemotherapeutic purposes.
Yeruva
Laxmi
L
Chemistry Department, University of Nevada, Las Vegas, Nevada, USA. vlyeruva@uams.edu
Elegbede
John Abiodun
JA
Carper
Stephen W
SW
D000085:Acetates / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D003517:Cyclopentanes / Q000494:pharmacology*; D053938:DNA Fragmentation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000096:biosynthesis / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects; D014410:Tumor Stem Cell Assay
D000085:Acetates; D000972:Antineoplastic Agents, Phytogenic; D003517:Cyclopentanes; D054883:Oxylipins; D047888:Receptors, Tumor Necrosis Factor, Type I; C072239:methyl jasmonate; D020928:Mitogen-Activated Protein Kinases; D053148:Caspase 3; D053181:Caspase 8