title: Deletion of the mouse RegIIIbeta (Reg2) gene disrupts ciliary neurotrophic factor signaling and delays myelination of mouse cranial motor neurons.
abstract: A large number of cytokines and growth factors support the development and subsequent maintenance of postnatal motor neurons. RegIIIbeta, also known as Reg2 in rat and HIP/PAP1 in humans, is a member of a family of growth factors found in many areas of the body and previously shown to play an important role in both the development and regeneration of subsets of motor neurons. It has been suggested that RegIIIbeta expressed by motor neurons is both an obligatory intermediate in the downstream signaling of the leukemia inhibitory factor/ciliary neurotrophic factor (CNTF) family of cytokines, maintaining the integrity of motor neurons during development, as well as a powerful influence on Schwann cell growth during regeneration of the peripheral nerve. Here we report that in mice with a deletion of the RegIIIbeta gene, motor neuron survival was unaffected up to 28 weeks after birth. However, there was no CNTF-mediated rescue of neonatal facial motor neurons after axotomy in KO animals when compared with wild-type. In mice, RegIIIbeta positive motor neurons are concentrated in cranial motor nuclei that are involved in the patterning of swallowing and suckling. We found that suckling was impaired in RegIIIbeta KO mice and correlated this with a significant delay in myelination of the hypoglossal nerve. In summary, we propose that RegIIIbeta has an important role to play in the developmental fine-tuning of neonatal motor behaviors mediating the response to peripherally derived cytokines and growth factors and regulating the myelination of motor axons.
authors.affiliation: National Centre for Cancer Research, Melchor Fernández Almagro, 3. E-28029 Madrid, Spain.
authors.forename: L A
authors.initials: LA
authors.lastname: Tebar
authors.forename: S M
authors.initials: SM
authors.lastname: Géranton
authors.forename: C
authors.initials: C
authors.lastname: Parsons-Perez
authors.forename: A S
authors.initials: AS
authors.lastname: Fisher
authors.forename: R
authors.initials: R
authors.lastname: Bayne
authors.forename: A J H
authors.initials: AJ
authors.lastname: Smith
authors.forename: M
authors.initials: M
authors.lastname: Turmaine
authors.forename: S
authors.initials: S
authors.lastname: Perez-Luz
authors.forename: A
authors.initials: A
authors.lastname: Sheasby
authors.forename: C
authors.initials: C
authors.lastname: De Felipe
authors.forename: C
authors.initials: C
authors.lastname: Ruff
authors.forename: G
authors.initials: G
authors.lastname: Raivich
authors.forename: S P
authors.initials: SP
authors.lastname: Hunt
mesh_terms: D000818:Animals; D020934:Ciliary Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D003679:Deglutition / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D007002:Hypoglossal Nerve / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009046:Motor Neurons / Q000378:metabolism*; D009186:Myelin Sheath / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D013391:Sucking Behavior / Q000502:physiology
chemical_list: D020934:Ciliary Neurotrophic Factor; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; C000612141:REG3A protein, human; C490253:Reg3b protein, mouse