Antioxidant activity of Aulosira fertilisima on CCl4 induced hepatotoxicity in rats.
Free radicals cause cell injury, when they are generated in excess or when the antioxidant defense is impaired. Carbon tetrachloride (CCl4) is used as a model for liver injury. In this study antioxidant activity of ethanol extract of A. fertilisima (EEA) was investigated using CCl4 intoxicated rat liver as the experimental model. Oral administration of EEA at a dose of 100 mg/kg body weight, for 14 consecutive days, the rate of the production of antioxidant enzymes like super oxide dismutase, catalase, glutathione peroxidase and glutathione transferase in rats compared to the CCl4 treated group without any supporting treatment. Liver damage is detected by the measurement of the activities of serum enzymes like aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transpeptidase and alkaline phosphatase which were released in to the blood from damaged cells. The normalization of these enzymes levels was observed in rats treated with EEA (100 mg/kg body weight) by reducing the leakage of the above enzymes in to the blood. The findings provide a rationale for further studies on isolation of active principles and its pharmacological evaluation. Protection offered by silymarin (standard reference drug) seemed relatively greater.
Kuriakose
Gini C
GC
Applied Biochemistry Research Laboratory, School of Biosciences, Mahatma Gandhi University, P.D. Hills P.O., Kottayam 686560, India. ginibesant@rediffmail.com
Kurup
G Muraleedhara
GM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001835:Body Weight; D002252:Carbon Tetrachloride Poisoning / Q000628:therapy*; D000458:Cyanobacteria / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D010865:Pilot Projects; D010936:Plant Extracts / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012838:Silymarin / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D010936:Plant Extracts; D012838:Silymarin; D000431:Ethanol; D013482:Superoxide Dismutase