title: [Preparation and identification of antibody against Cecropin-XJ].
abstract: OBJECTIVE
  To prepare the mouse antibody against Cecropin-XJ, identify its specificity and use it in cellular localization of Cecropin-XJ in vitro.
  
  
  METHODS
  The cDNA sequence of Cecropin-XJ with signal peptide sequence was subcloned into the eukaryotic expression vector pcDNA3. The recombinant plasmid was named pcDNA3-Cecropin-XJ, which was used as an antigen to immune the Kunming white mouse. Meanwhile, the cDNA sequence was subcloned into the fusion expression vector and the fusion protein was expressed as a test antigen.
  
  
  RESULTS
  Indirect ELISA showed that the fifth immunity's titer was highest. The immune gold-conjugated experiment showed that the prepared antibody was clearly and efficiently localized in prokaryotic cells where the Cecropin-XJ worked.
  
  
  CONCLUSIONS
  The antibody against Cecropin-XJ has high immune reactivity and specificity, which is beneficial to further study on Cecropin-XJ function and antibody preparation of small peptides.
authors.affiliation: Key Laboratory of Molecule Biology, College of Life Science and Technology, Xinjiang University, China. zst824@163.com
authors.forename: Shu-Tao
authors.initials: ST
authors.lastname: Zheng
authors.forename: Zhong-Yuan
authors.initials: ZY
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Dou
authors.forename: Fu-Chun
authors.initials: FC
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D054807:Cecropins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D007074:Immunoglobulin G / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D046529:Microscopy, Electron, Transmission
chemical_list: D000906:Antibodies; D054807:Cecropins; D018076:DNA, Complementary; D007074:Immunoglobulin G