title: Antifibrotic activity of diterpenes from Biota orientalis leaves on hepatic stellate cells.
abstract: Antifibrotic effect of twelve diterpenes (1-12) from the 90% methanolic fraction of Biota orientalis leaves was evaluated employing HSC-T6 cells by assessing cell proliferation and morphological change. Among these diterpenes, totarol (8) and isopimara-8(14),15-dien-19-oic acid (9) dramatically reduced cell proliferation in dose-and time-dependent manner. Furthermore, treatment with these compounds resulted in the different pattern of morphological changes of HSC-T6 cells. Taken together, antiproliferative activity of diterpenes from B. orientalis might suggest therapeutic potentials against liver fibrosis.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.forename: Hyekyung
authors.initials: H
authors.lastname: Yang
authors.forename: Jeong Seon
authors.initials: JS
authors.lastname: Yoon
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Jeong
authors.forename: Do Yoon
authors.initials: DY
authors.lastname: Kim
authors.forename: Na Ry
authors.initials: NR
authors.lastname: Ha
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D029779:Cupressaceae / Q000737:chemistry*; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008103:Liver Cirrhosis / Q000473:pathology / Q000517:prevention & control*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats
chemical_list: D004224:Diterpenes; D007770:L-Lactate Dehydrogenase