title: Using enzymatic amplification by aldolase for the optical detection of DNA by an artificial signal cascade.
abstract: A two-step reaction cascade is applied to the sequence-specific detection of single-stranded DNA, including analyte-triggered re-activation of apo-aldolase by its cofactor Zn(2+) and catalytic conversion of a chromophore.
authors.affiliation: Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Graf
authors.forename: Susanne
authors.initials: S
authors.lastname: Kassube
authors.forename: Roland
authors.initials: R
authors.lastname: Krämer
mesh_terms: D002384:Catalysis; D002413:Cations, Divalent; D004271:DNA, Fungal / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004789:Enzyme Activation; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry; D055095:Optics and Photonics; D011725:Pyridines / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D015398:Signal Transduction; D015032:Zinc
chemical_list: D002413:Cations, Divalent; D004271:DNA, Fungal; D004277:DNA, Single-Stranded; D011725:Pyridines; D005634:Fructose-Bisphosphate Aldolase; D015032:Zinc; C023666:pyridine