Physical and functional interactions between hematopoietic cell-specific ETS transcription factors and homeodomain proteins.
To examine the possibility that ETS family transcription factors, PU.1, SPI-B, ELF-1, ERG-3, ETS-1 and TEL, and homeodomain proteins, HOXA10, HOXC13, MEIS1 and PBX1B, function cooperatively, we investigated their interactions. In luciferase assays, HOXA10 and HOXC13 augmented the activity of PU.1 and SPI-B while diminishing that of ELF-1 and ERG-3. MEIS1 diminished the activity of ETS-1. No clear effects were observed for other combinations. Immunoprecipitation assays showed protein-protein interactions among the combinations exhibiting functional interactions. A mutation of HOXC13, which abolished binding to ELF-1, also abolished the diminishing effect on ELF-1. The results suggest functional interaction through physical interactions.
Yamada
Toshiyuki
T
Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
Shimizu
Takeshi
T
Sakurai
Takuya
T
Nanashima
Naoki
N
Kihara-Negishi
Fumiko
F
Suzuki
Mitsuhiro
M
Fan
Yang
Y
Akita
Miki
M
Oikawa
Tsuneyuki
T
Tsuchida
Shigeki
S
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells; D018398:Homeodomain Proteins / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D051379:Mice; D011485:Protein Binding / Q000502:physiology; D050783:Proto-Oncogene Proteins c-ets / Q000378:metabolism* / Q000502:physiology
D018398:Homeodomain Proteins; D050783:Proto-Oncogene Proteins c-ets