Analysis of stem cell lipids by offline HPTLC-MALDI-TOF MS.
MALDI-TOF MS is traditionally used for "proteomics", but is also a useful tool for lipid analysis. Depending on the applied matrix, however, some lipid classes are more sensitively detected than other ones and this may even lead to suppression effects if complex mixtures are analyzed. Therefore, a previous separation into the individual lipid classes is necessary. Using artificial lipid mixtures or easily available tissue extracts, it has been already shown that HPTLC-(High Performance Thin-Layer Chromatography)-separated lipids can be conveniently analyzed by MALDI-TOF MS directly on the TLC plate. Here we present an initial TLC-MALDI study of the lipid composition of ovine mesenchymal stem cells. Due to the complex composition of these cells, data are also compared to lipids extracted from human erythrocytes. It will be shown that even very minor lipid classes can be easily detected and with much higher sensitivity than by common staining protocols. Additionally, MS images of the developed TLC plates will be shown and potential applications, new methods of data analysis as well as problems discussed.
Fuchs
Beate
B
Faculty of Medicine, Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstrasse 16-18, 04107, Leipzig, Germany.
Schiller
Jürgen
J
Süss
Rosmarie
R
Zscharnack
Matthias
M
Bader
Augustinus
A
Müller
Peter
P
Schürenberg
Martin
M
Becker
Michael
M
Suckau
Detlev
D
D000818:Animals; D001853:Bone Marrow / Q000737:chemistry* / Q000502:physiology; D002855:Chromatography, Thin Layer / Q000379:methods*; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000502:physiology; D012756:Sheep; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D008055:Lipids