title: Characterization of the gene encoding glycoprotein C of duck enteritis virus.
abstract: A total of 2,718 bp of DNA fragment was amplified from the C-KCE strain of duck enteritis virus (DEV) genome using thermal asymmetric interlaced PCR. This newly identified viral DNA fragment contained two non-overlapping open reading frames (ORFs) oriented from the 5' to 3' direction. The first ORF was comprised of 43.5% G + C and contained the full-length genomic sequence of the UL44 gene (1,296 bp) encoding 431 amino acid residues of DEV glycoprotein C (gC). The second ORF encoded a partial peptide of the UL43 gene. The sequences of DNA and deduced amino acids of the DEV gC gene shared high homology with other members of known herpesviruses, supporting the classification of DEV. Phylogenetic analysis of the DEV gC gene revealed that the gC gene had a close evolutionary relationship with the subfamily of Alphaherpesvirinae.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yi Han
authors.initials: YH
authors.lastname: Wu
authors.forename: XiaoMei
authors.initials: X
authors.lastname: Liu
authors.forename: JunWei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002478:Cells, Cultured; D002645:Chickens; D016386:Chromosome Walking; D003001:Cloning, Molecular; D004372:Ducks; D019143:Evolution, Molecular; D006564:Herpesviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1