title: Intracellular uptake and photodynamic activity of water-soluble [60]- and [70]fullerenes incorporated in liposomes.
abstract: Water-soluble fullerenes have attracted attention as promising compounds that have been used to forge new paths in the field of photo-biochemistry. To prepare water-soluble fullerenes, we employed lipid-membrane-incorporated fullerenes (LMICx; x=60 or 70) by using the fullerene exchange method from a gamma-cyclodextrin (gamma-CD) cavity to vesicles. LMIC60 have low toxicity in the dark and engender cell death by photoirradiation (lambda>350 nm). Furthermore, the photodynamic activity of LMIC70 is 4.7-fold that of LMIC60 for the same photon flux (lambda>400 nm). One of the reasons for the higher phototoxicity of LMIC70 is the higher generation of singlet oxygen (1O2) in LMIC70 than in LMIC60. The difference between LMIC60 and LMIC70 is considered to be simply derived from the amount of light absorption in the 400-700 nm region that is suitable for photodynamic therapy (PDT). To the best of our knowledge, this is the first case in which biological activity of C70 and its derivatives toward HeLa cells has been assayed.
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan), Fax: (+81) 743-72-6091.|Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585 (Japan).
authors.forename: Yuki
authors.initials: Y
authors.lastname: Doi
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan), Fax: (+81) 743-72-6091.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ikeda
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan), Fax: (+81) 743-72-6091.
authors.forename: Motofusa
authors.initials: M
authors.lastname: Akiyama
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan), Fax: (+81) 743-72-6091.
authors.forename: Mai
authors.initials: M
authors.lastname: Nagano
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan), Fax: (+81) 743-72-6091.
authors.forename: Tamami
authors.initials: T
authors.lastname: Shigematsu
authors.affiliation: Graduate School of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan).
authors.forename: Takuya
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: Graduate School of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192 (Japan).
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Takeya
authors.affiliation: Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585 (Japan).
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagasaki
mesh_terms: D037741:Fullerenes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D015394:Molecular Structure; D010316:Particle Size; D010777:Photochemistry; D026082:Singlet Oxygen / Q000032:analysis / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D037741:Fullerenes; D008081:Liposomes; D014867:Water; D026082:Singlet Oxygen