Caenorhabditis elegans mutant allele identification by whole-genome sequencing.
Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.
Sarin
Sumeet
S
Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University Medical Center, New York, New York 10032, USA.
Prabhu
Snehit
S
O'Meara
M Maggie
MM
Pe'er
Itsik
I
Hobert
Oliver
O
D000483:Alleles; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D018509:DNA, Helminth / Q000235:genetics; D049751:Genome, Helminth; D009154:Mutation; D020641:Polymorphism, Single Nucleotide
D018509:DNA, Helminth