title: [Promoter activity of B-cell maturation antigen gene in mice].
abstract: OBJECTIVE
  To investigate the promoter activity of BCMA gene 5'-flanking regionfor the study the regulation mechanism of BCMA expression.
  
  
  METHODS
  Luciferase reporter plasmids containing 5'-flanking region of BCMA gene and serial deletions of the fragment were constructed. The luciferase expression was observed after these reporters were transfected into J558L, 293T and Hela cells.
  
  
  RESULTS
  The highest transcriptional activation was expressed in pGL3-B157 reporter, followed by pGL3-B607, pGL3-B359, pGL3-B93, and pGL3-B820 in sequence. The highest transcriptional activation was in J558L cell.
  
  
  CONCLUSIONS
  The 5'-flanking sequence from -820 to +145 bp of BCMA is of promoter activity. Serial deletion analysis of the promoter region of BCMA gene suggests the sequence from -93 to +145 could be a core promoter region.
authors.affiliation: Department of Medical Lab. Daping Hospital, Third Military Medical University, Chongqing 400038, China. dengl072@yahoo.com.cn
authors.forename: Shao-Li
authors.initials: SL
authors.lastname: Deng
authors.forename: Xiao-Xing
authors.initials: XX
authors.lastname: Cheng
authors.forename: Rui
authors.initials: R
authors.lastname: Jian
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000818:Animals; D053301:B-Cell Maturation Antigen / Q000235:genetics*; D002460:Cell Line; D006801:Humans; D051379:Mice; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics; D014162:Transfection
chemical_list: D053301:B-Cell Maturation Antigen