Differential involvement of GABA system in mediating behavioral and neurochemical effect of acupuncture in ethanol-withdrawn rats.
In our previous study we demonstrated that acupuncture at Shenmen (HT7) points suppressed a decrease of accumbal dopamine (DA) release in ethanol-withdrawn rats. Furthermore, here we found that it inhibited behavioral withdrawal signs of ethanol. In an effort to better understand the mechanisms underlying this inhibition, the potential role of GABA receptor system in acupuncture was investigated. Male Sprague-Dawley rats were treated with 3g/kg/day of ethanol (20%, w/v) or saline by intraperitoneal injection for 21 days. Following 48 or 72h of ethanol withdrawal, acupuncture was applied at bilateral HT7 for 1min. The selective GABA(A) antagonist bicuculline and the selective GABA(B) antagonist SCH 50911 were injected intraperitoneally 20min before acupuncture, respectively. Importantly, suppressive effects of acupuncture on DA deficiency were completely abolished by SCH 50911, but not by bicuculline, whereas ameliorating effects of acupuncture on ethanol withdrawal syndrome were completely blocked either by SCH 50911 or bicuculline. These results suggest that acupuncture at specific acupoint HT7 may normalize the DA release in the mesolimbic system and attenuate withdrawal syndrome through the GABA(B) receptor system in ethanol-withdrawn rats.
Lee
Bong Hyo
BH
College of Oriental Medicine, Daegu Haany University, Daegu, South Korea.
Zhao
Rong Jie
RJ
Moon
Jin Young
JY
Yoon
Seong Shoon
SS
Kim
Jung-Ae
JA
An
Heeduk
H
Kwon
Young Kyu
YK
Hwang
Meeyul
M
Choi
Seong Hun
SH
Shim
Insop
I
Kim
Bong Hyun
BH
Yang
Chae Ha
CH
D026881:Acupuncture / Q000379:methods*; D015669:Acupuncture Points; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D001640:Bicuculline / Q000494:pharmacology; D001923:Brain Chemistry / Q000502:physiology*; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D000431:Ethanol / Q000009:adverse effects*; D018682:GABA Agents / Q000494:pharmacology; D008297:Male; D009025:Morpholines / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013375:Substance Withdrawal Syndrome / Q000209:etiology* / Q000534:rehabilitation*; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
C095804:(+)-(S)-5,5-dimethylmorpholinyl-2-acetic acid; D018682:GABA Agents; D009025:Morpholines; D000431:Ethanol; D005680:gamma-Aminobutyric Acid; D004298:Dopamine; D001640:Bicuculline