title: Crystallization and preliminary crystallographic analysis of recombinant immunoglobulin G-binding protein from Streptococcus suis.
abstract: Streptococcus suis, an important zoonotic pathogen, expresses immunoglobulin G-binding protein, which is thought to be helpful to the organism in eluding the host defence system. Recombinant IgG-binding protein expressed in Escherichia coli has been crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 38.98, b = 43.94, c = 78.17 A and one molecule in the asymmetric unit. Diffraction data were collected to 2.60 A resolution.
authors.affiliation: Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: Abdul Hamid
authors.initials: AH
authors.lastname: Khan
authors.forename: Fuliang
authors.initials: F
authors.lastname: Chu
authors.forename: Youjun
authors.initials: Y
authors.lastname: Feng
authors.forename: Qinagmin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jianxun
authors.initials: J
authors.lastname: Qi
authors.forename: George Fu
authors.initials: GF
authors.lastname: Gao
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D017011:Streptococcus suis / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D011994:Recombinant Proteins