title: Spin-on end-functional diblock copolymers for quantitative DNA immobilization.
abstract: We demonstrate a simple means to covalently bond DNA to both hard (i.e., glass and silicon wafers) and soft (i.e., polymeric) substrates that provides quantitative and precise control of the DNA areal density. The approach is based on spin coating an alkyne-end-functional diblock copolymer, alpha-alkyne-omega-Br-poly( tBA- b-MMA), that self-assembles on both types of substrates as an ordered monolayer and thereby directs alkyne groups to the surface. Azido-functionalized DNA is covalently linked to the alkyne functionalized substrates by means of a "click" reaction between azide and alkyne groups. The density of immobilized DNA can be quantitatively controlled by varying the parameters used for spin-coating the copolymer film, that is, solution concentration and rotational speed, or by varying the copolymer molecular weight. We find the yield of the DNA coupling reaction to be dependent on the nature of the polymer underlying the reactive alkyne functional groups, being higher for more hydrophilic polymers.
authors.affiliation: Columbia University Department of Chemical Engineering 500 West 120th Street, New York, New York 10027, USA.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Hernán R
authors.initials: HR
authors.lastname: Rengifo
authors.forename: Cristian
authors.initials: C
authors.lastname: Grigoras
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Li
authors.forename: Zengmin
authors.initials: Z
authors.lastname: Li
authors.forename: Jingyue
authors.initials: J
authors.lastname: Ju
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Koberstein
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D008567:Membranes, Artificial; D008768:Methylmethacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D010777:Photochemistry; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000480:Alkynes; D001391:Azo Compounds; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D004247:DNA; D012825:Silicon