Validation of an ion trap tandem mass spectrometric analysis of mulberry 1-deoxynojirimycin in human plasma: application to pharmacokinetic studies.
Mulberry 1-deoxynojirimycin (DNJ) is a potent alpha-glucosidase inhibitor. Although it is useful for the treatment of diabetes, the human absorption and metabolism of DNJ have never been characterized. We developed a method using hydrophilic interaction chromatography coupled with ion trap tandem mass spectrometry, and found that orally administered DNJ was absorbed into the blood and then excreted into the urine.
Nakagawa
Kiyotaka
K
Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. nkgw@biochem.tohoku.ac.jp
Kubota
Hiroyuki
H
Tsuzuki
Tsuyoshi
T
Kariya
Jun
J
Kimura
Toshiyuki
T
Oikawa
Shinichi
S
Miyazawa
Teruo
T
D017485:1-Deoxynojirimycin / Q000097:blood* / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D007477:Ions / Q000737:chemistry; D015394:Molecular Structure; D031625:Morus / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D007477:Ions; D017485:1-Deoxynojirimycin