title: Reduction in rat oocyte fertilizability mediated by S-(1, 2-dichlorovinyl)-L-cysteine: a trichloroethylene metabolite produced by the glutathione conjugation pathway.
abstract: Trichloroethylene (TCE), a commonly used industrial degreasing solvent and environmental toxicant, reduces rat oocyte fertilizability by an incompletely understood mechanism. Previous evidence implicated cytochrome P450 dependent oxidation of TCE. The current study investigated a second pathway, glutathione conjugation using S-(1,2-dichlorovinyl)-L-cysteine (DCVC), a mutagenic and cytotoxic TCE-metabolite. In vitro exposure of oocytes and in vivo exposure of females to DCVC significantly reduced oocyte fertilizability (63% vs. 26%; p < 0.005 and 60% vs. 36%; p < 0.005, respectively). Reduced fertilizability of oocytes following in vivo TCE exposure may be mediated partially by the glutathione conjugation pathway.
authors.affiliation: Department of Animal Science, University of California, Davis, CA 95616, USA.
authors.forename: Katherine Lily
authors.initials: KL
authors.lastname: Wu
authors.forename: Trish
authors.initials: T
authors.lastname: Berger
mesh_terms: D000818:Animals; D001711:Biotransformation; D001835:Body Weight / Q000187:drug effects; D003545:Cysteine / Q000031:analogs & derivatives* / Q000633:toxicity; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects; D012997:Solvents; D014241:Trichloroethylene / Q000493:pharmacokinetics*
chemical_list: D012997:Solvents; D014241:Trichloroethylene; C039961:S-(1,2-dichlorovinyl)cysteine; D005978:Glutathione; D003545:Cysteine