title: Quantitative analysis of the intramacrophagic Brucella suis proteome reveals metabolic adaptation to late stage of cellular infection.
abstract: A 2-D DIGE approach allowed the characterization of the intramacrophagic proteome of the intracellular pathogen Brucella suis at the late stage of in vitro infection by efficient discrimination between bacterial and host cell proteins. Using a subtraction model, a total of 168 proteins showing altered concentrations in comparison with extracellularly grown, stationary-phase bacteria were identified. The majority of the 44 proteins significantly regulated at this stage of infection were involved in bacterial metabolism and 40% were present in lowered concentrations, supporting the hypothesis of an adaptive response by quantitative reduction of processes participating in energy, protein, and nucleic acid metabolism. In the future, the 2-D DIGE-based approach will permit to decipher specifically and quantitatively the intracellular proteomes of various pathogens during adaptation to their specific host cell environments.
authors.affiliation: Centre d'Etudes d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Université Montpellier 1, Montpellier, France.
authors.forename: Sascha
authors.initials: S
authors.lastname: Al Dahouk
authors.forename: Véronique
authors.initials: V
authors.lastname: Jubier-Maurin
authors.forename: Holger C
authors.initials: HC
authors.lastname: Scholz
authors.forename: Herbert
authors.initials: H
authors.lastname: Tomaso
authors.forename: Wolfram
authors.initials: W
authors.lastname: Karges
authors.forename: Heinrich
authors.initials: H
authors.lastname: Neubauer
authors.forename: Stephan
authors.initials: S
authors.lastname: Köhler
mesh_terms: D000818:Animals; D041021:Brucella suis / Q000378:metabolism*; D002006:Brucellosis / Q000382:microbiology*; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome