Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology.
We have developed a Linnik-type interference microscope provided with a low-coherent light source to obtain topographic images of an intact cellular membrane on a nanometer scale. Our technique is based on measurement of the interference between light reflected from the cell surface and a reference beam. The results show full field surface topography of cultured cells and reveal an intrinsic membrane motion of tens of nanometers.
Yamauchi
Toyohiko
T
Hamamatsu Photonics K. K., 5000 Hirakuchi, Hamamatsu-City, Shizuoka-Pref. 434-8601, Japan. t-yamauchi@crl.hpk.co.jp
Iwai
Hidenao
H
Miwa
Mitsuharu
M
Yamashita
Yutaka
Y
D002462:Cell Membrane / Q000648:ultrastructure*; D048429:Cell Size; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods