title: Influence of protein malnutrition on induction and treatment of inflammation in mice.
abstract: Considerable evidence suggests that protein malnutrition (PM) impairs immune function especially cytokine and interferes with the toxicopharmacology of some non-steroidal anti-inflammatory drugs. The present study tries to assess whether PM will affect induction and treatment of inflammation. The study was performed on adult male Swiss mice using the carrageenan-air pouch model. Animals were fed either protein sufficient 20% or protein deficient 8% casein diet starting 3wks before induction of air pouch, 6-days after which the pouch was administered with 1ml carrageenan (2%). Treatment with aspirin (200 mg/kg, p.o.) or diclofenac (20 mg/kg im.) was conducted one hour before the challenge with carrageenan. Six hours after the challenge, the local exudates were collected and subjected for total leukocyte infiltration count and IL-12 assessment. IL-1 beta and nitrate content were estimated in both plasma and air pouch infiltrate. The obtained results showed a reduction in leukocytes and decrease in IL-1 beta level in PM mice. In conclusion, PM slows the inflammatory reaction, and influences the inflammatory process differently especially in the presence of another stimulus.
authors.affiliation: National Organization for Drug Control and Research (NODCAR), Cairo, Egypt.
authors.forename: Nahed M A
authors.initials: NM
authors.lastname: Hassanein
authors.forename: Roba M
authors.initials: RM
authors.lastname: Talaat
authors.forename: Khalid
authors.initials: K
authors.lastname: Bassiouny
authors.forename: M Raouf
authors.initials: MR
authors.lastname: Hamed
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D001241:Aspirin / Q000627:therapeutic use; D002351:Carrageenan; D004008:Diclofenac / Q000627:therapeutic use; D018753:Diet, Protein-Restricted; D004195:Disease Models, Animal; D007249:Inflammation / Q000139:chemically induced / Q000150:complications* / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D018664:Interleukin-12 / Q000097:blood*; D053583:Interleukin-1beta / Q000097:blood*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D044342:Malnutrition / Q000150:complications / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D053583:Interleukin-1beta; D004008:Diclofenac; D018664:Interleukin-12; D002351:Carrageenan; D001241:Aspirin