Optimization of extraction and purification of active fractions from Schisandra chinensis (Turcz.) and its osteoblastic proliferation stimulating activity.
Extraction and purification conditions of lignans from the fruits and seeds of Schisandra chinensis (Turcz.) were investigated through an orthogonal design of L(9)(3(4)) assay and macroporous resin technology. The extraction was optimized using 95% ethanol. For purification, the extract was dissolved in 30% ethanol, then adsorbed on a AB-8 macroporous resin and eluted with 30% ethanol and 70% ethanol successively, the latter resulting in a residue containing 65.2% of lignans. By HPLC analysis schisandrin, deoxyschisandrin and gamma-schisandrin were quantitatively determined. UMR 106 cells were used to examine the stimulatory activity of the lignans on osteoblasts in vitro. The lignans stimulated the proliferation of and the activity of alkaline phosphatase in the osteoblasts indicating their potential activity against osteoporosis.
Caichompoo
Wanida
W
School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
Zhang
Qiao-Yan
QY
Hou
Ting-Ting
TT
Gao
Hua-Juan
HJ
Qin
Lu-Ping
LP
Zhou
Xiu-Jia
XJ
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D034242:Cyclooctanes / Q000302:isolation & purification* / Q000494:pharmacology; D020536:Enzyme Activators / Q000302:isolation & purification / Q000494:pharmacology; D017705:Lignans / Q000302:isolation & purification* / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011083:Polycyclic Compounds / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D032141:Schisandra / Q000737:chemistry*
D034242:Cyclooctanes; D020536:Enzyme Activators; D017705:Lignans; D010936:Plant Extracts; D011083:Polycyclic Compounds; C015499:schizandrin B; C034734:schizandrin A; D000469:Alkaline Phosphatase; C011105:schizandrin