title: Expression of canine Kdap in normal, hyperplastic and neoplastic epidermis.
abstract: Keratinocyte differentiation-associated protein, Kdap, is a recently identified small secretory protein that may act as a soluble regulator for the cornification and/or desquamation of keratinocytes. To clarify the role of Kdap in the terminal differentiation of keratinocytes, detailed in situ localisation of Kdap was studied using canine skin with normal, hyperplastic and neoplastic epidermis. In normal canine trunk skin, Kdap was expressed by granular keratinocytes, with polarity to the apical side of the cells, suggesting that canine Kdap is present in lamellar granules, as in humans. Expression of Kdap was widespread in the spinous layers in hyperplastic epidermis, but was undetectable in squamous cell carcinomas. These findings suggest that Kdap is closely related to the delay of terminal differentiation and/or release of cells in hyperplastic epidermis.
authors.affiliation: Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yagihara
authors.forename: Emiko
authors.initials: E
authors.lastname: Kinjo
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Ayaka
authors.initials: A
authors.lastname: Tsuji
authors.forename: Yudai
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Nao
authors.initials: N
authors.lastname: Shinozaki
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Tamura
authors.forename: Mayu
authors.initials: M
authors.lastname: Isotani
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Nakagaki
authors.forename: Kimimasa
authors.initials: K
authors.lastname: Takahashi
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ono
authors.forename: Tsukimi
authors.initials: T
authors.lastname: Washizu
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
mesh_terms: D000818:Animals; D002460:Cell Line; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006965:Hyperplasia / Q000201:enzymology / Q000235:genetics / Q000662:veterinary*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012878:Skin Neoplasms / Q000201:enzymology* / Q000235:genetics
chemical_list: D010750:Phosphoproteins; D012333:RNA, Messenger