[Human bone marrow stromal cells facilitate the cord blood CD34+ cells ex vivo expansion and short-term engraftment in NOD/SCID mice].
OBJECTIVE
To explore the potential of human bone marrow stromal cells (MSCs) as the feeding-layer to promote ex vivo expansion of cord blood CD34+ cells and engraftment of the expanded cells in NOD/SCID mice.


METHODS
Human MSCs were routinely isolated and cultured. MSCs at passage 3 were used as feeding-layer for the expansion of cord blood CD34+ cell in the presence of thrombopoietin (TPO), flt3/flk2 ligand (FL), stem cell factor (SCF) and granulocyte-colony stimulating factor (G-CSF). The engraftment potential between unexpanded and expanded cord blood cells transplanted into NOD/SCID mice was compared.


RESULTS
The total nucleated cells (TNC), CD34 cells and colony forming units (CFUs) in the MSC feeding culture were increased by 111.6-, 19.3- and 58-fold after 1 week expansion and 532.8-, 41.3- and 563.5- fold increased after 2 weeks expansion respectively as compared with that in non MSC feeding culture. In transplant experiment, the percentage of human CD45+ cells (45.3% -59.1%) in bone marrow of recipient mice transplanted with the MSC feeding expanded cells was the highest in all the groups at six weeks after transplantation.


CONCLUSIONS
Human MSCs enhance CB CD34+ cells in vitro expansion and their capacity of short-term engraftment in NOD/SCID mice.
Fei
Xiao-Ming
XM
Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Wu
Yu-Jie
YJ
Chang
Zai
Z
Miao
Kou-Rong
KR
Zhou
Xiao-Yu
XY
Pan
Qin-Qin
QQ
Wang
Cheng-Ya
CY
D000818:Animals; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D002469:Cell Separation; D002478:Cells, Cultured; D036101:Cord Blood Stem Cell Transplantation; D005312:Fetal Blood / Q000166:cytology*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID
D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor