title: Mesoporous silica nanoparticles as a delivery system of gadolinium for effective human stem cell tracking.
abstract: The progress of using gadolinium (Gd)-based nanoparticles in cellular tracking lags behind that of superparamagnetic iron oxide (SPIO) nanoparticles in magnetic resonance imaging (MRI). Here, dual functional Gd-fluorescein isothiocyanate mesoporous silica nanoparticles (Gd-Dye@MSN) that possess green fluorescence and paramagnetism are developed in order to evaluate their potential as effective T1-enhancing trackers for human mesenchymal stem cells (hMSCs). hMSCs are labeled efficiently with Gd-Dye@MSN via endocytosis. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes, osteocytes, and chondrocytes, which can still be readily MRI detected. Imaging, with a clinical 1.5-T MRI system and a low incubation dosage of Gd, low detection cell numbers, and short incubation times is demonstrated on both loaded cells and hMSC-injected mouse brains. This study shows that the advantages of biocompatibility, durability, high internalizing efficiency, and pore architecture make MSNs an ideal vector of T1-agent for stem-cell tracking with MRI.
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Jong-Kai
authors.initials: JK
authors.lastname: Hsiao
authors.forename: Chih-Pin
authors.initials: CP
authors.lastname: Tsai
authors.forename: Tsai-Hua
authors.initials: TH
authors.lastname: Chung
authors.forename: Yann
authors.initials: Y
authors.lastname: Hung
authors.forename: Ming
authors.initials: M
authors.lastname: Yao
authors.forename: Hon-Man
authors.initials: HM
authors.lastname: Liu
authors.forename: Chung-Yuan
authors.initials: CY
authors.lastname: Mou
authors.forename: Chung-Shi
authors.initials: CS
authors.lastname: Yang
authors.forename: Yao-Chang
authors.initials: YC
authors.lastname: Chen
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Huang
mesh_terms: D002478:Cells, Cultured; D016503:Drug Delivery Systems; D005682:Gadolinium / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008279:Magnetic Resonance Imaging; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D012822:Silicon Dioxide; D005682:Gadolinium