Effect of phospholipid insertion on arrayed polydiacetylene biosensors.
Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition.
Kim
Kyung-Woo
KW
Department of Chemical and Biological Engineering and Center for Integrated-Nano Systems, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, Republic of Korea.
Choi
Hyun
H
Lee
Gil Sun
GS
Ahn
Dong June
DJ
Oh
Min-Kyu
MK
D000577:Amides / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002264:Carboxylic Acids / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry; D005453:Fluorescence; D005898:Glass / Q000737:chemistry; D006860:Hydrogen Bonding; D008958:Models, Molecular; D015394:Molecular Structure; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D040081:Protein Array Analysis; D012486:Salmonella typhimurium / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D000577:Amides; D000906:Antibodies; D002264:Carboxylic Acids; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D004134:Dimyristoylphosphatidylcholine