[Establishment and characteristics of acute lung injury model induced by cigarette smoke in ICR mice].
To develop a mouse model of acute lung injury induced by cigarette smoke (CS) and to investigate inflammatory changes with the model.



ICR mice exposed to CS for 20-min, 3/d. Bronchoalveolar lavage fluid (BALF) and lung tissue were harvested at d 0, d 1, d 3 and d 7 after CS exposure. Neutrophil count in BAFL, TNF-alpha and MMP-12 levels, the activity of MPO in lung tissue were determined.



Neutrophil count in BALF, MMP-12 and MPO levels in lung tissue were increased after CS exposure in a time-dependent manner with a peak at d3. TNF-alpha level sharply increased at d1, and remained high level until d7.



ICR mice are tolerant and sensitive to CS exposure, which may be used as an appropriate animal model for acute lung injury induced by cigarette smoke.
Wang
Qin-chuan
QC
College of Medicine, Zhejiang University,Hangzhou 310058, China.
Chen
Yu
Y
Tang
Hui-fang
HF
Tang
Jin-fei
JF
Lu
Jian-ju
JJ
Chen
Ji-qiang
JQ
D055371:Acute Lung Injury / Q000139:chemically induced* / Q000473:pathology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004195:Disease Models, Animal; D008297:Male; D053508:Matrix Metalloproteinase 12 / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D012906:Smoke; D014409:Tumor Necrosis Factor-alpha; D053508:Matrix Metalloproteinase 12