Quality evaluation of umbilical cord blood progenitor cells cryopreserved with a small-scale automated liquid nitrogen system.
The performance of a small-scale automated cryopreservation and storage system (Mini-BioArchive system) used in the banking of umbilical cord blood (UCB) units was evaluated. After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34+ cells, colony-forming unit-granulocyte-macrophage (CFU-GM), and total CFU were analyzed. Twenty UCB units cryopreserved using the automated system and stored for a median of 34 days were analyzed. Mean CD34+ cell viabilities before freezing were 99.8+/-0.5% and after thawing were 99.8+/-0.4% in the large bag compartments and 99.7+/-0.5% in the small compartments. The mean recovery values for total nucleated cells, CD34+ cells, CFU-GM, and total CFU were 94.8+/-16.0%, 99.3+/-18.6%, 103.9+/-20.6%, and 94.3+/-12.5%, respectively in the large compartments, and 95.8+/-25.9%, 106.8+/-23.9%, 101.3+/-23.3%, and 93.8+/-19.2%, respectively in the small compartments. A small-scale automated cryopreservation and storage system did not impair the clonogenic capacity of UCB HPCs. This cryopreservation system could provide cellular products adequate for UCB banking and HPC transplantation.
Miura
Junko
J
Division of Blood Transfusion, Tohoku University Hospital, 1-1 Seiryomachi, Aoba-ku, Sendai, Miyagi Prefecture 980-8574, Japan.
Minegishi
Masayoshi
M
Itoh
Tsuneo
T
Kitaura
Tamie
T
Fukawa
Narumi
N
Takahashi
Hiroyuki
H
Suzuki
Akira
A
Kudo
Yoshinori
Y
Narita
Ayuko
A
Sato
Yuko
Y
Suzuki
Masakuni
M
Wada
Yuichi
Y
Takeyama
Yoichi
Y
Watanabe
Takanori
T
Tsuchiya
Shigeru
S
D018952:Antigens, CD34; D001771:Blood Banks; D001793:Blood Preservation; D002470:Cell Survival; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003114:Colony-Forming Units Assay; D015925:Cryopreservation / Q000295:instrumentation* / Q000379:methods; D003911:Dextrans; D004121:Dimethyl Sulfoxide; D005260:Female; D005312:Fetal Blood; D005434:Flow Cytometry; D005615:Freezing; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D009584:Nitrogen; D013997:Time Factors
D018952:Antigens, CD34; D003911:Dextrans; D009584:Nitrogen; D004121:Dimethyl Sulfoxide