title: Toward the authentication of wines of Nemea denomination of origin through cleaved amplified polymorphic sequence (CAPS)-based assay.
abstract: In the present study, we developed a cleaved amplified polymorphic sequence (CAPS)-based assay as a first attempt to detect fraud in grapevine musts with a long-term objective to establish an analytical methodology to authenticate wines of Nemea denomination of origin (Agiorgitiko). The analytical assay makes use of a single nucleotide polymorphism that discriminates Agiorgitiko and Cabernet Sauvignon varieties. The latter grape variety is one of the major adulterants for Nemea wines. Agiorgitiko grapevine must was spiked with Cabernet Sauvignon in several ratios (v/v) from 50 down to 10%, and the subsequent mixes were subjected to alcoholic microfermentation. DNA was extracted from all mixture samples up to the end of the fermentation process and was subjected to the CAPS assay. Both standard agarose gel and lab-on-a-chip capillary electrophoresis illustrated the ability of the method to detect the presence of Cabernet Sauvignon down to 10% throughout the whole fermentation process.
authors.affiliation: Division of Nutritional Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leics LE12 5RD, United Kingdom. s_spaniolas@yahoo.com
authors.forename: Stelios
authors.initials: S
authors.lastname: Spaniolas
authors.forename: Maroussa
authors.initials: M
authors.lastname: Tsachaki
authors.forename: Malcolm J
authors.initials: MJ
authors.lastname: Bennett
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Tucker
mesh_terms: D018744:DNA, Plant / Q000032:analysis* / Q000737:chemistry; D005285:Fermentation; D005506:Food Contamination / Q000032:analysis; D005638:Fruit / Q000235:genetics*; D006115:Greece; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D027843:Vitis / Q000235:genetics*; D014920:Wine / Q000032:analysis / Q000145:classification*
chemical_list: D018744:DNA, Plant