title: In vitro and in vivo effect of lidocaine on rat muscle-derived cells for treatment of stress urinary incontinence.
abstract: OBJECTIVE
  Lidocaine cytotoxicity has been reported in some cell types, which could affect its use as a local anesthetic in cell-based therapy. We evaluated the in vitro and in vivo effect of lidocaine on rat muscle-derived progenitor cells (MDCs).
  
  
  METHODS
  MDCs were isolated from rat skeletal muscle and purified using the preplate technique. For in vitro tests, the MDCs underwent either 2 hours of, or continuous, exposure to lidocaine (50 microM-5 mM). After 72 hours of incubation, cell viability was measured using the methylthiazololetetrazolium assay. For the in vivo tests, periurethral injection of either phosphate-buffered saline, MDCs (1 x 10(6) cells/20 microL), or 2% lidocaine plus MDCs was performed in pudendal nerve-transected rats. The leak point pressure (LPP) was measured at 4 weeks after the injection.
  
  
  RESULTS
  Lidocaine concentrations of < or = 500 microM had no effect on MDCs with continuous exposure. MDCs in 1 mM lidocaine showed decreased survival and no MDCs in 5 mM lidocaine survived. With a 2-hour exposure, only MDCs in the 5-mM lidocaine group showed decreased survival. Rats with nerve transection and phosphate-buffered saline injection showed significantly lower LPPs than the controls. The LPP was restored to a significantly greater level after MDCs only or lidocaine plus MDC injection. No statistically significant difference in LPP restoration was found between the MDC-only and lidocaine plus MDC injections.
  
  
  CONCLUSIONS
  Cytotoxicity to lidocaine was minimal at a physiologic concentration in vitro. The functional recovery of LPP by MDC treatment was not affected by lidocaine preinfiltration. Taken together, our data indicate that lidocaine can be applied as a local anesthetic in periurethral MDC injection without decreasing the efficacy of the therapy.
authors.affiliation: Department of Urology, Eulji University, Daejeon, Republic of Korea.
authors.forename: Dae Kyung
authors.initials: DK
authors.lastname: Kim
authors.forename: Ron J
authors.initials: RJ
authors.lastname: Jankowski
authors.forename: Ryan
authors.initials: R
authors.lastname: Pruchnic
authors.forename: Fernando
authors.initials: F
authors.lastname: de Miguel
authors.forename: Naoki
authors.initials: N
authors.lastname: Yoshimura
authors.forename: Masashi
authors.initials: M
authors.lastname: Honda
authors.forename: Akira
authors.initials: A
authors.lastname: Furuta
authors.forename: Michael B
authors.initials: MB
authors.lastname: Chancellor
mesh_terms: D000779:Anesthetics, Local / Q000009:adverse effects / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D008012:Lidocaine / Q000009:adverse effects / Q000494:pharmacology*; D032342:Muscle Cells / Q000187:drug effects* / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014550:Urinary Incontinence, Stress / Q000601:surgery*
chemical_list: D000779:Anesthetics, Local; D008012:Lidocaine