title: Bioactive 5alpha-pregnane-type steroidal alkaloids from Sarcococca hookeriana.
abstract: The bioassay-guided phytochemical investigation of Sarcococca hookeriana with respect to cholinesterase inhibitory properties has yielded two new 5alpha-pregnane-type steroidal alkaloids, hookerianamides J (1) and K (2), along with eight known compounds (3-10). The structures of 1 and 2 were elucidated by spectroscopic methods. These compounds displayed good to moderate activities in vitro against the enzymes acetylcholinesterase (IC 50 8.1-48.5 microM) and butyrylcholinesterase (IC 50 0.4-4.0 microM). Compounds 1-10 were also tested in vitro for their leishmanicidal activity against Leishmania major and for their antibacterial activities against Bacillus subtilis, Micrococcus luteus, Streptococcus faecalis, and Pseudomonas pallida.
authors.affiliation: Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, P.O. Box 100131, 33501 Bielefeld, Germany. devkotakpd@yahoo.com
authors.forename: Krishna P
authors.initials: KP
authors.lastname: Devkota
authors.forename: Bruno N
authors.initials: BN
authors.lastname: Lenta
authors.forename: Jean D
authors.initials: JD
authors.lastname: Wansi
authors.forename: Muhammad I
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Kisangau
authors.forename: Qamar
authors.initials: Q
authors.lastname: Naz
authors.lastname: Samreen
authors.forename: Norbert
authors.initials: N
authors.lastname: Sewald
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D031234:Buxaceae / Q000737:chemistry*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D011278:Pregnanes / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000981:Antiprotozoal Agents; D002800:Cholinesterase Inhibitors; D011278:Pregnanes