Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma.
MicroRNAs are a group of small non-coding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature microRNAs in human cancers. We characterized the alteration in expression of a select group of microRNAs in primary peritoneal carcinoma relative to matched cases of ovarian serous carcinoma. MicroRNA expression was analysed using semi-quantitative stem-loop RT-PCR on a set of 34 formalin-fixed paraffin-embedded samples. Protein expression of p53 and bcl-2 was quantified in the corresponding tissue microarray. We provide definitive evidence that there is downregulation of a select group of microRNAs in tumours meeting Gynaecological Oncology Group criteria for primary peritoneal carcinoma relative to ovarian serous carcinoma. Specifically, we show decreased p53 expression and downregulation of miR-195 and miR-497 from the microRNA cluster site at chromosome 17p13.1 in primary peritoneal carcinoma relative to ovarian serous carcinoma. miR-195 and miR-497 may have potential roles as tumour-suppressor genes in primary peritoneal tumourigenesis.
Flavin
Richard J
RJ
Department of Histopathology, Trinity College Dublin, Dublin, Ireland. flavinr@tcd.ie
Smyth
Paul C
PC
Laios
Alexandros
A
O'Toole
Sharon A
SA
Barrett
Ciara
C
Finn
Stephen P
SP
Russell
Susan
S
Ring
Martina
M
Denning
Karen M
KM
Li
Jinghuan
J
Aherne
Sinead T
ST
Sammarae
Dania A
DA
Aziz
Natasha A
NA
Alhadi
Araibi
A
Sheppard
Brian L
BL
Lao
Kai
K
Sheils
Orla M
OM
O'Leary
John J
JJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D002886:Chromosomes, Human, Pair 17; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D010534:Peritoneal Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
C505362:MIRN16 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53