A protein domain-based interactome network for C. elegans early embryogenesis.
Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.
Boxem
Mike
M
Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. mboxem@partners.org
Maliga
Zoltan
Z
Klitgord
Niels
N
Li
Na
N
Lemmens
Irma
I
Mana
Miyeko
M
de Lichtervelde
Lorenzo
L
Mul
Joram D
JD
van de Peut
Diederik
D
Devos
Maxime
M
Simonis
Nicolas
N
Yildirim
Muhammed A
MA
Cokol
Murat
M
Kao
Huey-Ling
HL
de Smet
Anne-Sophie
AS
Wang
Haidong
H
Schlaitz
Anne-Lore
AL
Hao
Tong
T
Milstein
Stuart
S
Fan
Changyu
C
Tipsword
Mike
M
Drew
Kevin
K
Galli
Matilde
M
Rhrissorrakrai
Kahn
K
Drechsel
David
D
Koller
Daphne
D
Roth
Frederick P
FP
Iakoucheva
Lilia M
LM
Dunker
A Keith
AK
Bonneau
Richard
R
Gunsalus
Kristin C
KC
Hill
David E
DE
Piano
Fabio
F
Tavernier
Jan
J
van den Heuvel
Sander
S
Hyman
Anthony A
AA
Vidal
Marc
M
D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology*; D002455:Cell Division; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D020543:Proteome; D020798:Two-Hybrid System Techniques
D020543:Proteome