title: Effects of basic fibroblast growth factor on in vitro development of human ovarian primordial follicles.
abstract: OBJECTIVE
  To evaluate whether basic fibroblast growth factor (FGF) benefits the in vitro development of human primordial follicles.
  
  
  METHODS
  Human ovarian tissue was placed in organ culture for 4 weeks with basic FGF and either fetal calf serum or a serum-free combination. Control groups were cultured with a neutralizing antibody against basic FGF.
  
  
  METHODS
  Major tertiary care and referral academic centers.
  
  
  METHODS
  Fourteen women/girls undergoing various gynecological operations and two fetuses from women undergoing pregnancy terminations.
  
  
  METHODS
  None.
  
  
  METHODS
  Follicular counts, immunohistochemistry for proliferating cell nuclear antigen and bromodeoxyuridine incorporation and measurement of 17beta E(2) production.
  
  
  RESULTS
  Only in the serum-free culture system was the number of developing follicles in samples cultured with basic FGF significantly higher than in uncultured specimens. The E(2) production increased significantly in the second week, and there was a significant reduction in E(2) secretion with the addition of the neutralizing antibody against basic FGF. The percentage of granulosa cells (GCs) that stained for proliferating cell nuclear antigen or bromodeoxyuridine was significantly higher in developing follicles than in primordial follicles, regardless of treatment.
  
  
  CONCLUSIONS
  Basic FGF apparently plays a role in the E(2) production of early follicles. High doses of basic FGF enhanced follicular development in serum-free media.
authors.affiliation: Infertility and IVF Unit, Rabin Medical Center, Petach Tikva, Israel.
authors.forename: Roni
authors.initials: R
authors.lastname: Garor
authors.forename: Ronit
authors.initials: R
authors.lastname: Abir
authors.forename: Arye
authors.initials: A
authors.lastname: Erman
authors.forename: Carmela
authors.initials: C
authors.lastname: Felz
authors.forename: Shmuel
authors.initials: S
authors.lastname: Nitke
authors.forename: Benjamin
authors.initials: B
authors.lastname: Fisch
mesh_terms: D000028:Abortion, Induced; D000293:Adolescent; D000328:Adult; D001706:Biopsy; D018929:Cell Culture Techniques / Q000379:methods; D002648:Child; D015925:Cryopreservation; D016895:Culture Media, Serum-Free; D004958:Estradiol / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D006080:Ovarian Follicle / Q000166:cytology* / Q000187:drug effects; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000473:pathology; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D055815:Young Adult
chemical_list: D016895:Culture Media, Serum-Free; D018809:Proliferating Cell Nuclear Antigen; D016222:Fibroblast Growth Factor 2; D004958:Estradiol