Anti-inflammatory activity of Acanthus ilicifolius.
Acanthus ilicifolius Linn, is a perennial herb (Acanthaceae) widely found in the Sundarban mangroves and is popularly used for its wound healing effects. In the present study an attempt was made to evaluate the anti-inflammatory activity of the Acanthus ilicifolius leaves. The methanolic fraction of Acanthus ilicifolius leaf extract produced significant inhibition of rat paw oedema, when administered both prior to and after carrageenan administration, in a manner similar to BW755C a synthetic cyclooxygenase (COX) and lipoxygenase (LOX) inhibitor. The extract decreased protein exudation and leukocyte migration in the peritoneal fluid, thereby indicating its effectiveness towards inhibiting peritoneal inflammation. It also produced significant inhibition of COX (1 and 2) and 5-LOX activity. Preincubation of the extract inhibited the production of proinflammatory cytokines (TNFalpha and IL-6) in lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs). The methanolic fraction of the extract was also found to possess significant free radical (DPPH, ABTS, superoxide and hydroxyl radical) scavenging activity. The extract on intraperitoneal administration augmented the endogenous antioxidant status, as evident from the significant increase of ferric reducing ability of plasma (FRAP) and total peroxyl radical trapping activity of plasma (TRAP).
Mani Senthil Kumar
K T
KT
School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, West Bengal, India.
Gorain
Bapi
B
Roy
Dilip K
DK
Zothanpuia
Samanta
Samir K
SK
Pal
Mintu
M
Biswas
Prova
P
Roy
Amrita
A
Adhikari
Dipan
D
Karmakar
Sanmoy
S
Sen
Tuhinadri
T
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine / Q000494:pharmacology; D027901:Acanthaceae / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002351:Carrageenan; D002465:Cell Movement / Q000187:drug effects; D016861:Cyclooxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D016207:Cytokines / Q000187:drug effects / Q000378:metabolism; D004487:Edema / Q000188:drug therapy*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D007194:India; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D051381:Rats
D000893:Anti-Inflammatory Agents; D016861:Cyclooxygenase Inhibitors; D016207:Cytokines; D016166:Free Radical Scavengers; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D002351:Carrageenan