The actin propulsive machinery: the proteome of Listeria monocytogenes tails.
Actin-based comet tails produced by Listeria monocytogenes are considered as representative models for cellular force-producing machineries crucial for cell migration. We here present a proteomic picture of these tails formed in extracts from brain and platelets. This provides a comprehensive view, revealing high molecular complexity and novel host cell proteins as tail components, and suggests the participation of specific multicomponent regulatory complexes. This work forms a new basis to expand current models of cellular protrusion.
Van Troys
Marleen
M
Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium.
Lambrechts
Anja
A
David
Violaine
V
Demol
Hans
H
Puype
Magda
M
Pizarro-Cerda
Javier
J
Gevaert
Kris
K
Cossart
Pascale
P
Vandekerckhove
Joël
J
D000199:Actins / Q000378:metabolism*; D001792:Blood Platelets / Q000382:microbiology; D001921:Brain / Q000382:microbiology; D002148:Calmodulin-Binding Proteins / Q000378:metabolism; D002465:Cell Movement; D006367:HeLa Cells; D006801:Humans; D008089:Listeria monocytogenes / Q000502:physiology*; D008088:Listeriosis / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D020543:Proteome
D000199:Actins; D002148:Calmodulin-Binding Proteins; D009479:Neuropeptides; D020543:Proteome; C053386:drebrins