title: Confinement effects on the interaction of native DNA with Cu(II)-5-(triethylammoniummethyl)salicylidene ortho-phenylendiiminate in C(12)E(4) liquid crystals.
abstract: Confinement effects of native calf thymus DNA interacting with the complex Cu(ii)-5-(triethylammoniummethyl)salicylidene ortho-phenylendiiminate (CuL(2+)) perchlorate in tetraethylene glycol monododecyl ether (C(12)E(4)) liquid crystals have been investigated by UV absorption spectrophotometry, circular dichroism (CD) and small angle X-ray scattering (SAXS). The results indicate the occurrence of dramatic structural changes of both the DNA and the CuL(2+)-DNA system, when going from aqueous solution to C(12)E(4) liquid crystals, due to confinement constrains imposed by the closed structure of C(12)E(4) reverse micelles. Further marked departures from the behaviour observed in aqueous solution have been emphasized by registering the spectral response of DNA and CuL(2+)-DNA confined in C(12)E(4) reverse micelles after thermal treatment. It has been also ascertained that the confinement causes the formation of a more compact and thermoresistant DNA structure accompanied by a transition from the right- to left-handed form while a tight CuL(2+)-DNA binding has been revealed by the appearance of a broad induced CD band in the range 350-450 nm. From a biological point of view, these findings stress the need to account for confinement effects and the peculiarity of drug-DNA interactions occurring within the intra-cellular environment.
authors.affiliation: Dipartimento di Chimica Inorganica e Analitica S. Cannizzaro, Università di Palermo, Viale delle Scienze, Parco d'Orleans II, Edificio 17, 90128, Palermo, Italy.
authors.forename: Giampaolo
authors.initials: G
authors.lastname: Barone
authors.forename: Alessandro
authors.initials: A
authors.lastname: Longo
authors.forename: Angela
authors.initials: A
authors.lastname: Ruggirello
authors.forename: Arturo
authors.initials: A
authors.lastname: Silvestri
authors.forename: Alessio
authors.initials: A
authors.lastname: Terenzi
authors.forename: Vincenzo Turco
authors.initials: VT
authors.lastname: Liveri
mesh_terms: D000042:Absorption; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism; D050866:Liquid Crystals / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry*; D053838:Scattering, Small Angle; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: C543744:Cu(II)-5-(triethylammoniummethyl)salicylidene ortho-phenylendiiminate; D009942:Organometallic Compounds; D014867:Water; D011092:Polyethylene Glycols; C053131:dodecyltetraethylene glycol monoether; D004247:DNA