title: Algorithm and simulation for analysis of bio-images obtained by aperture diffraction based optical MEMS.
abstract: This paper proposes a novel method to detect transparent living cells in a transparent microfluidic chamber by optical diffraction of an aperture or an aperture array. Through the analysis of the far-field diffraction pattern, one of the parameters of the cells, including the size, refractive index, or position, can be extracted by the analysis software developed in this paper. Calculations are carried out to discuss the key issues of this MEMS device, and our simulation is verified by diffraction patterns of transparent microparticles on fabricated apertures, recorded via a digital camera.
authors.affiliation: Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602. donna-zhou@imre.a-star.edu.sg
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhou
authors.forename: Daniel Puiu
authors.initials: DP
authors.lastname: Poenar
authors.forename: Kai Yu
authors.initials: KY
authors.lastname: Liu
authors.forename: Man Siu
authors.initials: MS
authors.lastname: Tse
authors.forename: Chew-Kiat
authors.initials: CK
authors.lastname: Heng
authors.forename: Swee Ngin
authors.initials: SN
authors.lastname: Tan
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
mesh_terms: D000465:Algorithms; D002478:Cells, Cultured / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012031:Refractometry / Q000379:methods*