[Preparation of hemoglobin-loaded nanoparticles and safety evaluation in vitro and in vivo].
Hemoglobin-loaded nano-sized particles with oxygen carrying capacity were prepared. All experiments were performed using biodegradable polymer poly (polyepsilon-caprolactone) (PCL) as matrix polymer. Optimized preparation parameters led to nanoparticles with well-defined characteristics such as size <200 nm, P50 27 mmHg and high encapsulation efficiency up to 99.4%. The results of in vitro and vivo studies suggested that Hb-loaded particles did not activate complements. After the nanoparticles suspension was injected into the mice via tail vein, the particles did not cause significant changes in total platelet counts. Apparently, the hemoglobin-loaded nanoparticles can serve as a potential candidate in substitution for red cells.
Zhao
Jian
J
Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China.
Shan
Xiaoqian
X
Sheng
Yan
Y
Wu
Fan
F
Yuan
Yuan
Y
Liu
Changsheng
C
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001802:Blood Substitutes / Q000138:chemical synthesis* / Q000737:chemistry; D002417:Cattle; D006454:Hemoglobins / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*
D001672:Biocompatible Materials; D001802:Blood Substitutes; D006454:Hemoglobins; D011091:Polyesters; C016240:polycaprolactone