Association of estrogen receptor alpha and interleukin-10 gene polymorphisms with endometriosis in a Chinese population.
OBJECTIVE
To determine whether polymorphisms of the estrogen receptor alpha (ERalpha) and interleukin-10 (IL-10) genes are associated with endometriosis in a Chinese population.


METHODS
Association study.


METHODS
University hospital.


METHODS
Chinese women diagnosed with endometriosis by laparotomy or laparoscopy.


METHODS
Determination of polymorphisms of the ERalpha and IL-10 genes was performed by polymerase chain reaction and restriction fragment length polymorphism analysis in 214 affected women and 160 controls.


METHODS
Frequency and distribution of PvuII and XbaI polymorphisms of ERalpha and of BslI, SspI, and RsaI polymorphisms of IL-10.


RESULTS
There was no significant difference between the endometriosis patients and the control groups in the genotype frequency of ERalpha-PvuII and promoter of IL-10 gene polymorphisms in the position of -1082. However, the frequency of ERalpha-XbaI and -592 or -819C alleles of IL-10 in the endometriosis group was significantly higher than that of controls, and further analysis showed that the X allele of ERalpha-XbaI was associated with endometriosis.


CONCLUSIONS
The X allele of ERalpha-XbaI and the -592 or -819 C allele of IL-10 are associated with endometriosis.
Xie
Jingyan
J
Central Laboratory of Nanjing First Hospital affiliated to Nanjing Medical University, Nanjing, People's Republic of China.
Wang
Shukui
S
He
Bangshun
B
Pan
Yuqin
Y
Li
Yujuan
Y
Zeng
Qingdi
Q
Jiang
Huaxin
H
Chen
Jialiang
J
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D002681:China; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004715:Endometriosis / Q000235:genetics* / Q000601:surgery*; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D010535:Laparoscopy; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012016:Reference Values; D055815:Young Adult
D017931:DNA Primers; D047628:Estrogen Receptor alpha; D016753:Interleukin-10; D004247:DNA