Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells.
Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20 mM glucose, and varied from 1.0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h.
Azzu
Vian
V
MRC Dunn Human Nutrition Unit, Wellcome Trust/MRC Building, Cambridge, UK. va@mrc-dunn.cam.ac.uk
Affourtit
Charles
C
Breen
Eamon P
EP
Parker
Nadeene
N
Brand
Martin D
MD
D000818:Animals; D045744:Cell Line, Tumor / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007340:Insulinoma / Q000378:metabolism*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D000071246:Uncoupling Protein 2
D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604389:UCP2 protein, human; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2; D005947:Glucose