DNA sequence analysis of varicella-zoster virus gene 62 from subclinical infections in healthy children immunized with the Oka varicella vaccine.
A live attenuated varicella vaccine, the Oka vaccine strain (vOka), is routinely administered to children in Japan and other countries, including the United States. vOka consists of a mixture of genotypically distinct variants, but little is known about the growth potential of each variants in vivo. We isolated varicella-zoster virus (VZV) DNA sequences from the peripheral blood mononuclear cells (PBMCs) of asymptomatic healthy children immunized with the Oka varicella vaccine. VZV gene 62 DNA fragments were detected in 5 of 166 (3.0%) PBMC samples by nested PCR within 5 weeks of the vaccination. Sequence analysis of VZV DNA from these five PBMC samples indicated that multiple viral clones in the vaccine could infect vaccinees and replicate in vivo. We also provide evidence that a nonsynonymous substitution at position 105356 may affect viral replication in vivo.
Gomi
Yasuyuki
Y
Kanonji Institute, Research Foundation for Microbial Diseases of Osaka University, 2-9-41, Yahata-cho, Kanonji, Kagawa, Japan. ygomi@mail.biken.or.jp
Ozaki
Takao
T
Nishimura
Naoko
N
Narita
Atsushi
A
Suzuki
Michio
M
Ahn
Jaekun
J
Watanabe
Naoko
N
Koyama
Norio
N
Ushida
Hajime
H
Yasuda
Naoko
N
Nakane
Kazumasa
K
Funahashi
Keiji
K
Fuke
Isao
I
Takamizawa
Akihisa
A
Ishikawa
Toyokazu
T
Yamanishi
Koichi
K
Takahashi
Michiaki
M
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002644:Chickenpox / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control / Q000821:virology*; D019433:Chickenpox Vaccine / Q000008:administration & dosage*; D002648:Child; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics*; D007114:Immunization; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*; D014779:Virus Replication
D000914:Antibodies, Viral; D019433:Chickenpox Vaccine; C074221:IE62 protein, Human herpesvirus 3; D017874:Immediate-Early Proteins; D015534:Trans-Activators; D014759:Viral Envelope Proteins