Human melanoma cells release soluble and functional receptors.
Cytokines play important roles in the growth and growth arrest of cancer cells. IL-13 via an IL-4R alpha/IL-13R alpha 1 heterocomplex receptor inhibits the growth of renal cell carcinoma cells (RCC). However, it does not inhibit the growth of glioblastoma cells that express the IL-13R alpha 2 chain. In the present studies we investigated whether melanoma cells express IL-13R alpha 1 and IL-13R alpha 2 chains as well as whether they respond to IL-13. Membrane IL13R alpha 2 was co-expressed with IL-4R alpha and IL-13R alpha 1 chains in three of six tested melanoma cell lines. Furthermore, the IL-13R alpha 2 positive cell lines, release a soluble form of IL-13R alpha 2, specifically under IL-13 but not IL-4 stimulation. The release of soluble IL-13R alpha 2 was inhibited by various metalloproteinase inhibitors and EDTA inhibits the biological response to IL-13.
Badr
G
G
Zoology Department, Faculty of Science, Assiut University, Assiut, Egypt.
Sayed
D
D
D045744:Cell Line, Tumor; D006801:Humans; D018793:Interleukin-13 / Q000276:immunology / Q000378:metabolism*; D053661:Interleukin-13 Receptor alpha1 Subunit / Q000276:immunology / Q000378:metabolism*; D053663:Interleukin-13 Receptor alpha2 Subunit / Q000276:immunology / Q000378:metabolism*; D015847:Interleukin-4 / Q000276:immunology / Q000378:metabolism*; D008545:Melanoma / Q000276:immunology* / Q000378:metabolism; D045726:Metalloproteases / Q000378:metabolism; D019948:Receptors, Interleukin-4 / Q000276:immunology / Q000378:metabolism*; D012878:Skin Neoplasms / Q000276:immunology / Q000378:metabolism
D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D053663:Interleukin-13 Receptor alpha2 Subunit; D019948:Receptors, Interleukin-4; D015847:Interleukin-4; D045726:Metalloproteases