Glycine transporters (glycine transporter 1 and glycine transporter 2) are expressed in retina.
The amino acid glycine is an inhibitory neurotransmitter in the spinal cord, brain stem, and vertebrate retina. The effective synaptic concentrations of glycine are regulated by at least two transporters: glycine transporter 1 and glycine transporter 2. Here, we show retinal expression of glycine transporter 1 by in-situ hybridization and of glycine transporter 2 by reverse transcriptase-PCR and in-situ hybridization. In-situ hybridization signals were observed in the ganglionar and inner nuclear layer as well as in the outer nuclear layer of the frog and rat retinas. In addition, accumulation of H-glycine was observed in isolated photoreceptor cells. The expression of these transporters in nonglycinergic cells suggests that they may also modulate electrical signals.
Peña-Rangel
Ma Teresa
MT
Department of Developmental Neurobiology and Neurophysiology, Institute of Neurobiology, Universidad Nacional Autonoma de Mexico, Mexico.
Riesgo-Escovar
Juan R
JR
Sánchez-Chávez
Gustavo
G
Salceda
Rocío
R
D000818:Animals; D020869:Gene Expression Profiling; D050487:Glycine Plasma Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D017403:In Situ Hybridization; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D020419:Photoreceptor Cells, Vertebrate / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011894:Rana pipiens; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D050487:Glycine Plasma Membrane Transport Proteins; D012333:RNA, Messenger; C493269:Slc6a5 protein, rat; C493266:Slc6a9 protein, rat