title: Microwave-assisted aqueous two-phase extraction of piceid, resveratrol and emodin from Polygonum cuspidatum by ethanol/ammonium sulphate systems.
abstract: Microwave-assisted, aqueous two-phase extraction was investigated to obtain effective constituents, including piceid, resveratrol and emodin in Polygonum cuspidatum. An aqueous two-phase system consisting of 25% (w/w) ethanol 21% (w/w) (NH(4))(2)SO(4) gave equal yields of piceid, and 1.1- and 1.9-times higher yields of resveratrol and emodin, respectively, than that achieved by microwave-assisted extraction and heat reflux extraction. Three-separate operations, extraction, clarification and concentration, are hereby integrated into a single step to get higher yields at lower cost. This is therefore a potentially useful method for the extraction and purification of target products.
authors.affiliation: Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Yuesheng
authors.initials: Y
authors.lastname: Dong
authors.forename: Zhi-Long
authors.initials: ZL
authors.lastname: Xiu
mesh_terms: D000097:Acetonitriles; D000645:Ammonium Sulfate; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004642:Emodin / Q000302:isolation & purification*; D000431:Ethanol; D045468:Fallopia japonica / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification*; D008872:Microwaves; D010936:Plant Extracts / Q000302:isolation & purification; D000077185:Resveratrol; D013267:Stilbenes / Q000302:isolation & purification*
chemical_list: D000097:Acetonitriles; D005960:Glucosides; D010936:Plant Extracts; D013267:Stilbenes; D000431:Ethanol; D004642:Emodin; D000077185:Resveratrol; D000645:Ammonium Sulfate; C058229:polydatin; C032159:acetonitrile