[Achievement and progress of dynamic culture conditions on the construction of engineered blood vessel substitutes].
Dynamic culture conditions serve as a critical factor in constructing physiologically functional blood vessel substitutes. With the development of tissue engineering, many experiments have verified the hypothesis that proper dynamic conditions are important for cells' behaving ways such as attachment, proliferation, differentiation and the remodeling of the extracellular matrix. By means of simulating the physiological dynamic conditions, one can enhance cell growth and extracellular matrix remodeling, and further improve the function of engineered vessel. In this paper is reviewed the current state of dynamic culture conditions provided for tissue engineering vessel graft, including the effects of different dynamic culture conditions on the proliferation of vascular cells and the remodeling of extracellular matrix. The use of bioreactor in constructing tissue engineering blood vessels and the effects of dynamic culture environments are also discussed with respect to the biological properties of vascular grafts.
Han
Zhaozhao
Z
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China.
Kong
Hua
H
Xu
Haiyan
H
D000818:Animals; D001696:Biomechanical Phenomena; D019149:Bioreactors; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D011651:Pulmonary Artery / Q000166:cytology*; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*