Chemical fingerprint and quantitative analysis of Salvia plebeia R.Br. by high-performance liquid chromatography.
To control the quality of Salvia plebeia R.Br., a simple and reliable method of high-performance liquid chromatography coupled with photodiode array detector (HPLC-DAD) was developed both for fingerprint analysis and quantitative determination of seven bioactive compounds, namely caffeic acid, luteolin-7-glucoside, nepetin-7-glucoside, homoplantaginin, luteolin, nepetin and hispidulin. In fingerprint analysis, twelve peaks were selected as characteristic peaks. In quantitative analysis, seven compounds showed good regression (R2>0.9995) within test ranges and the recovery of the method was in the range of 91.7-103.2%. The content ranges (mg/g) of seven compounds in the collected samples of S. plebeia were 0.80-1.67 (hispidulin), 2.18-5.75 (homoplantaginin), 0.52-1.22 (nepetin), 1.56-3.48 (nepetin-7-glucoside), 0.12-0.24 (luteolin), 0.97-2.22 (luteolin-7-glucoside) and 0.21-0.44 (caffeic acid), respectively. From the results obtained, the content of homoplantaginin was the highest. In addition, luteolin and luteolin-7-glucoside were isolated for the first time from S. plebeia.
Jin
Xiao-feng
XF
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.
Lu
Yan-hua
YH
Wei
Dong-zhi
DZ
Wang
Zheng-tao
ZT
D002109:Caffeic Acids / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D047309:Flavones / Q000032:analysis / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D005960:Glucosides / Q000032:analysis / Q000737:chemistry; D047311:Luteolin / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D027544:Salvia / Q000737:chemistry* / Q000145:classification*
C403862:6-methoxy-4',5-dihydroxyflavone-7-O-glucoside; D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D047309:Flavones; D005419:Flavonoids; D005960:Glucosides; D010936:Plant Extracts; C066408:luteolin-7-glucoside; D011794:Quercetin; C503624:eupafolin; D047311:Luteolin; C055957:hispidulin; C040048:caffeic acid