Identification of Curcuma plants and curcumin content level by DNA polymorphisms in the trnS-trnfM intergenic spacer in chloroplast DNA.
With the goal of developing an accurate plant identification method, molecular analysis based on polymorphisms of the nucleotide sequence of chloroplast DNA (cpDNA) was performed in order to distinguish four Curcuma species: C. longa, C. aromatica, C. zedoaria, and C. xanthorrhiza. Nineteen regions of cpDNA were amplified successfully via polymerase chain reaction (PCR) using total DNA of all Curcuma plants. Using the intergenic spacer between trnS and trnfM (trnSfM), all four Curcuma plant species were correctly identified. In addition, the number of AT repeats in the trnSfM region was predictive of the curcumin content in the rhizome of C. longa.
Minami
Motoyasu
M
Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan. minami@isc.chubu.ac.jp
Nishio
Kaori
K
Ajioka
Yui
Y
Kyushima
Hiroaki
H
Shigeki
Katsuki
K
Kinjo
Kaneo
K
Yamada
Kazuno
K
Nagai
Masashi
M
Satoh
Kiyoshi
K
Sakurai
Yozo
Y
D002851:Chromatography, High Pressure Liquid; D030024:Curcuma / Q000235:genetics* / Q000378:metabolism*; D003474:Curcumin / Q000378:metabolism*; D018742:DNA, Chloroplast / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012343:RNA, Transfer / Q000235:genetics
D018742:DNA, Chloroplast; D021901:DNA, Intergenic; D012343:RNA, Transfer; D003474:Curcumin