Disposable microscope objective lenses for fluorescence correlation spectroscopy using latex microspheres.
We explore the combination of a latex microsphere with a low NA lens to form a high performance optical system, and enable the detection of single molecules by fluorescence correlation spectroscopy (FCS). Viable FCS experiments at concentrations 1-1000 nM with different objectives costing less than $40 are demonstrated. This offers a simple and low-cost alternative to the conventional complex microscope objectives.
Wenger
Jérôme
J
Institut Fresnel, Aix-Marseille Université, CNRS, 13397 Marseille, France.
Gérard
Davy
D
Aouani
Heykel
H
Rigneault
Hervé
H
D003365:Costs and Cost Analysis; D004209:Disposable Equipment / Q000191:economics; D007840:Latex; D007909:Lenses / Q000191:economics; D008853:Microscopy / Q000191:economics / Q000295:instrumentation*; D008863:Microspheres; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D007840:Latex