title: Preparation of CLA ascorbyl ester with improved volumetric productivity by an ionic liquid-based reaction system.
abstract: A new approach to the enzymatic production of conjugated linoleic acid (CLA) ascorbyl ester with a remarkably high volumetric productivity (120-200 g L(-1)) has been developed, in which strong solvation by tOMA.TFA (methyltrioctylammonium trifluoroacetate) enables a high concentration of ascorbic acid to be applied, and in which t-butanol enhances conversion by changing the equilibrium constant of the activity coefficients. This work has experimentally demonstrated the practicability of achieving efficient reactions of polar compounds at high concentrations in ionic liquids without significant loss of enzyme activity.
authors.affiliation: National Food Institute, Technical University of Denmark, Building 227, DK-2800 KgsLyngby, Denmark.
authors.forename: Bilian
authors.initials: B
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Guo
authors.forename: Mette Bruni
authors.initials: MB
authors.lastname: Let
authors.forename: Bena-Marie
authors.initials: BM
authors.lastname: Lue
authors.forename: Xuebing
authors.initials: X
authors.lastname: Xu
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry*; D019149:Bioreactors; D002175:Candida / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D004951:Esterification; D004952:Esters / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids; D044243:Linoleic Acids, Conjugated / Q000737:chemistry / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics; D014269:Trifluoroacetic Acid / Q000737:chemistry; D020002:tert-Butyl Alcohol / Q000737:chemistry
chemical_list: C525963:1-butyl-3-methylimidazolium; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D007093:Imidazoles; D052578:Ionic Liquids; D044243:Linoleic Acids, Conjugated; D000644:Quaternary Ammonium Compounds; C037759:trioctylmethylammonium; D014269:Trifluoroacetic Acid; C093667:Novozyme 435; D008049:Lipase; D020002:tert-Butyl Alcohol; D001205:Ascorbic Acid