The extracellular proteoglycan produced by Rhodella grisea.
Highly viscous extracellular proteoglycan (EPG) has been isolated from culture medium of the unicellular red alga Rhodella grisea (Rhodophyceae) by ethanol precipitation. EPG was composed of xylose (29.3%), 3-O-methyl-xylose (26.0%), uronic acids (17.1%), rhamnose (14.4%), galactose (7.5%), glucose (3.9%), arabinose (1.4%) and mannose (0.4%), and traces of fucose, 4-O-methyl-xylose and 2,3-di-O-methyl-rhamnose or fucose. In addition, the polymer contained proteins (13.1%), sulphates and 13C-CP MAS spectra indicated the presence of acetyl and succinyl groups. The molecular mass was estimated to be 136,000. Ion-exchange chromatography afforded five fractions differing in composition of neutral sugars, uronic acids, and protein content indicating thus the complex structure of the EPG.
Capek
P
P
Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia. chemcape@savba.sk
Matulová
M
M
Combourieu
B
B
D005110:Extracellular Space / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011509:Proteoglycans / Q000096:biosynthesis* / Q000737:chemistry; D000461:Rhodophyta / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
D011509:Proteoglycans