title: Hypochlorous acid-induced modulation of cellular redox status in HeLa cells.
abstract: Myeloperoxidase catalyzes the formation of hypochlorous acid (HOCI) via reaction of H2O2 with CI(-) ions. Although HOCI plays a major role in the human immune system by killing bacteria and other invading pathogens, excessive generation of this oxidant causes damage to tissues. Exposure of HeLa cells to HOCI decreased viability, inactivated antioxidant enzymes, damaged mitochondria, and modulated cellular redox status. HOCI also induced significant increases in cellular oxidative damage reflected by lipid peroxidation, protein oxidation, and DNA damage. HOCI-mediated oxidative damage to HeLa cells may perturb the cellular antioxidant defense mechanisms and subsequently lead to a pro-oxidant state.
authors.affiliation: School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu, Korea.
authors.forename: Sin Young
authors.initials: SY
authors.lastname: Park
authors.forename: Seoung Woo
authors.initials: SW
authors.lastname: Shin
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Lee
authors.forename: Jeen-Woo
authors.initials: JW
authors.lastname: Park
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D006367:HeLa Cells; D006801:Humans; D006997:Hypochlorous Acid / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008931:Mitochondria, Muscle / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species; D013050:Spectrometry, Fluorescence; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D016877:Oxidants; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D006997:Hypochlorous Acid