title: Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice.
abstract: Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H(2)) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H(2), VC, or pure water (H(2)O), administered for 33 days. VC levels in H(2) and H(2)O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H(2) group subjected to ischemia-reperfusion than in the H(2)O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, Chiba 274-8510, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Sato
authors.forename: Shizuo
authors.initials: S
authors.lastname: Kajiyama
authors.forename: Akiko
authors.initials: A
authors.lastname: Amano
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Kondo
authors.forename: Toru
authors.initials: T
authors.lastname: Sasaki
authors.forename: Setsuko
authors.initials: S
authors.lastname: Handa
authors.forename: Ryoya
authors.initials: R
authors.lastname: Takahashi
authors.forename: Michiaki
authors.initials: M
authors.lastname: Fukui
authors.forename: Goji
authors.initials: G
authors.lastname: Hasegawa
authors.forename: Naoto
authors.initials: N
authors.lastname: Nakamura
authors.forename: Hikohito
authors.initials: H
authors.lastname: Fujinawa
authors.forename: Toyotaka
authors.initials: T
authors.lastname: Mori
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Ohta
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Obayashi
authors.forename: Naoki
authors.initials: N
authors.lastname: Maruyama
authors.forename: Akihito
authors.initials: A
authors.lastname: Ishigami
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000378:metabolism*; D001835:Body Weight; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D006859:Hydrogen / Q000494:pharmacology*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D018384:Oxidative Stress; D013481:Superoxides / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014867:Water / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C513324:Rgn protein, mouse; D014867:Water; D013481:Superoxides; D006859:Hydrogen; D002265:Carboxylic Ester Hydrolases; C020579:gluconolactonase; D001205:Ascorbic Acid