Induction of allergic contact dermatitis by astigmatid mite-derived monoterpene, alpha-acaridial.
alpha-Acaridial [2(E)-(4-methyl-3-pentenyl)butenedial] is a novel monoterpene secreted from the house dust mites. Because of its molecular nature of a highly reactive, small lipidic compound, we addressed whether alpha-acaridial might function as a haptenic allergen that induced allergic contact dermatitis. Mice sensitized with alpha-acaridial were challenged by the same antigen on the ear skin. After 2 days, significant ear swelling with a prominent infiltration of CD4(+) T lymphocytes was observed. In vitro, alpha-acaridial exhibited an outstanding ability to quickly interact with and chemically modify a reference protein. Virtually all cysteine residues and a sizable fraction of lysine residues were found to be selectively modified, suggesting that alpha-acaridial could potentially interact with any proteins. Previously, numerous mite-derived proteinaceous allergens have been associated with contact dermatitis. Our study now emphasizes that small lipidic compounds released from mites comprise a new class of mite allergens, and therefore, is of significant medical implications.
Sasai
Toshio
T
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan.
Hirano
Yunosuke
Y
Maeda
Sayaka
S
Matsunaga
Isamu
I
Otsuka
Atsushi
A
Morita
Daisuke
D
Nishida
Ritsuo
R
Nakayama
Hideo
H
Kuwahara
Yasumasa
Y
Sugita
Masahiko
M
Mori
Naoki
N
D000447:Aldehydes / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000737:chemistry / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D039821:Monoterpenes / Q000737:chemistry / Q000276:immunology*
D000447:Aldehydes; D039741:Antigens, Dermatophagoides; D039821:Monoterpenes; C448740:beta-acaridial