[Construction and identification of rat GDNF gene recombinant retroviral vector and gene transfection to NSC].
By genetic recombinant technique, the rat GDNF cDNA was recombinated to the retroviral vector pLXSN. The recombinant plasmid pLXSN-GDNF was verified by digestion with restriction endonucleases and PCR. Then neural stem cells (NSCs) were infected with pLXSN-GDNF. Immunocytochemistry, RT-PCR and western-blot were used to detect the transfection effect. Results showed that GDNF cDNA was cloned into retroviral vector pLXSN correctly, and the pLXSN-GDNF can infect NSCs efficiently. These results provide the possibility for transplantation and gene therapy with GDNF of nervous system diseases and injury.
Cheng
Saiyu
S
Department of Neurobiology, The Third Military Medical University, Chongqing Institute of Neuroscience, Chongqing 400038, China.
Ruan
Huaizhen
H
Yang
Zhong
Z
Wu
Xigui
X
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics*; D009474:Neurons / Q000166:cytology*; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics; D013234:Stem Cells / Q000166:cytology*
D018076:DNA, Complementary; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D011994:Recombinant Proteins