title: Detection and pharmacokinetics of alginate oligosaccharides in mouse plasma and urine after oral administration by a liquid chromatography/tandem mass spectrometry (LC-MS/MS) method.
abstract: A sensitive and simple liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed for the detection of alginate oligosaccharides (AOs) in mouse plasma and urine after oral administration. In an AO mixture, dimer, trimer, and tetramer were detected by LC-MS/MS equipped with an anion-exchange column with extremely high sensitivity. By this method, we detected certain levels of AOs in samples prepared from mouse plasma and urine after a single oral administration of the AO mixture. Based on a calibration curve made with an AO trimer peak area as a standard, the maximum plasma and urine concentrations of AOs were estimated to be 24.5 microg/ml at 5 min and 425.5 microg/ml at 30 min, respectively. These results suggest that the LC-MS/MS method is well suited to pharmacokinetic analysis of AOs in an in vivo system, and that some of orally administered AOs, at least from dimer to tetramer, are absorbed by digestive organs promptly, and that unaltered, these oligomers were excreted into an urine after a single oral administration to a mouse.
authors.affiliation: Nagasaki Prefectural Institute for Environment Research and Public Health, Omura, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Nishikawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yokose
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Oda
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D009844:Oligosaccharides / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D009844:Oligosaccharides; D020723:Glucuronic Acid