title: Effect of HbS in the determination of HbA2 with the Menarini HA-8160 analyzer and comparison with other instruments.
abstract: It is known that the presence of hemoglobin S (HbS) affects the determination of hemoglobin A(2) (HbA(2)) levels in clinical samples. We quantitated this effect using the Menarini HA-8160 analyzer and compared with other instruments (HELENA beta-thal quik column, TOSOH HLC-723G7 and BIORAD Variant II) using the HELENA SAS-MX alkaline gel electrophoresis kit as the reference method. The %HbA(2) values from the HA-8160 analyzer and the alkaline gel electrophoresis show a good linear correlation in the absence of HbS. A strong positive bias in the %HbA(2) values from the HA-8160 is apparent in the presence of HbS in the samples, when compared with the alkaline electrophoresis. The analytical imprecision and bias of the three HPLC instruments are comparable both in the presence and absence of HbS. The manual column method shows a lower bias in the absence of HbS but is more affected when HbS is present in the samples.
authors.affiliation: Laboratory of Biochemistry, Department of Medicine, Democritus University of Thrace, Alexandroupolis, Greece. kanagnos@med.duth.gr
authors.forename: K
authors.initials: K
authors.lastname: Anagnostopoulos
authors.forename: I
authors.initials: I
authors.lastname: Tentes
authors.forename: C
authors.initials: C
authors.lastname: Kalleas
authors.forename: D
authors.initials: D
authors.lastname: Margaritis
authors.forename: A
authors.initials: A
authors.lastname: Toli
authors.forename: D
authors.initials: D
authors.lastname: Pendilas
authors.forename: G
authors.initials: G
authors.lastname: Bourikas
authors.forename: C
authors.initials: C
authors.lastname: Tsatalas
authors.forename: A H
authors.initials: AH
authors.lastname: Kortsaris
mesh_terms: D057205:Automation, Laboratory; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D019075:Electrophoresis, Capillary; D005189:False Positive Reactions; D006443:Hemoglobin A2 / Q000032:analysis* / Q000302:isolation & purification; D006451:Hemoglobin, Sickle / Q000032:analysis*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D006451:Hemoglobin, Sickle; D011933:Reagent Kits, Diagnostic; D006443:Hemoglobin A2