Isolation and characterization of a Delta5-desaturase from Oblongichytrium sp.
We isolated a cDNA clone with homology to known desaturase genes from Oblongichytrium sp., recently classified as a new genus of thraustochytrids (Labyrinthulomycetes), and found that it encoded Delta5-desaturase by its heterologous expression in yeast. The enzyme had higher activity toward 20:4n-3 than 20:3n-6, indicating that this Delta5-desaturase can be used in the production of n-3 polyunsaturated fatty acids in transgenic organisms.
Kumon
Yasuyuki
Y
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Kamisaka
Yasushi
Y
Tomita
Nao
N
Kimura
Kazuyoshi
K
Uemura
Hiroshi
H
Yokochi
Toshihiro
T
Yokoyama
Rinka
R
Honda
Daiske
D
D000595:Amino Acid Sequence; D000818:Animals; D002849:Chromatography, Gas; D004951:Esterification; D005227:Fatty Acids / Q000737:chemistry; D008386:Marine Biology; D008745:Methylation; D008969:Molecular Sequence Data; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D005227:Fatty Acids; D044925:Oxidoreductases Acting on CH-CH Group Donors