Stimulation of human mesenchymal stem cells and osteoblasts activities in vitro on silicon-releasable scaffolds.
Cellular activities of human osteoblasts (HOBs) and mesenchymal stem cells (MSCs) on a silicon-releasable scaffold, siloxane-doped poly(lactic acid) and vaterite composite coated with hydroxycarbonate apatite (SPV-H), were estimated using a medium with or without organic factors, such as dexamethasone (Dex) and beta-glycerophosphate (beta-GP), for inducing mineralization or differentiation. As a control, a composite film containing no silicon (denoted by PV-H) was prepared using poly(lactic acid) and vaterite. HOBs cultured on SPV-H formed some agglomerates, bone nodules, after a 21-day culture in a medium without the organic factors, whereas no agglomerate was observed on PV-H. Laser Raman spectra implied that calcium phosphate precipitated in HOBs on the SPV-H. The silicon species in SPV-H stimulated HOBs to mineralization. The culture tests using MSCs show that the level of alkaline phosphatase (ALP) activity in the cells cultured on SPV-H increased during the 21-day culture in a medium without Dex and beta-GP. The level was unchanged in MSCs cultured on PV-H. In the case of supplementing Dex and beta-GP to the medium, the level of ALP activity in MSCs cultured on SPV-H was higher than that on PV-H at all time points during the 21-day culture. The silicon species in SPV-H were regarded to induce and enhance the osteogenic differentiation of MSCs.
Obata
Akiko
A
Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan. obata.akiko@nitech.ac.jp
Kasuga
Toshihiro
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D001031:Apatites / Q000737:chemistry*; D002113:Calcification, Physiologic; D002119:Calcium Carbonate / Q000737:chemistry; D002254:Carbonates / Q000737:chemistry*; D002478:Cells, Cultured; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D012833:Siloxanes / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry*
D001031:Apatites; D002254:Carbonates; D011091:Polyesters; D011108:Polymers; D012833:Siloxanes; C413465:hydroxycarbonate apatite; D019344:Lactic Acid; C033616:poly(lactide); D000469:Alkaline Phosphatase; D002119:Calcium Carbonate; D012825:Silicon