Protection of human HepG2 cells against oxidative stress by cocoa phenolic extract.
Cocoa is a rich source of flavanols and procyanidin oligomers with antioxidative properties, providing protection against oxidation and nitration. The present study investigated the potential protective effect of a polyphenolic extract from cocoa on cell viability and antioxidant defenses of cultured human HepG2 cells submitted to oxidative stress induced by tert-butylhydroperoxide (t-BOOH). Pretreatment of cells with 0.05-50 microg/mL of cocoa polyphenolic extract (CPE) for 2 or 20 h completely prevented cell damage and enhanced activity of antioxidant enzymes induced by a treatment with t-BOOH. Moreover, lower levels of GSH caused by t-BOOH in HepG2 cells were partly recovered by a pretreatment with CPE. Increased reactive oxygen species (ROS) induced by t-BOOH was dose-dependently prevented when cells were pretreated for 2 or 20 h with CPE. These results show that treatment of HepG2 in culture with CPE (within the physiological range of concentrations) confers a significant protection against oxidation to the cells.
Martín
María Angeles
MA
Department of Metabolism and Nutrition Instituto del Frío, Consejo Superior de Investigaciones Científicas, José Antonio Novais 10, 28040 Madrid, Spain.
Ramos
Sonia
S
Mateos
Raquel
R
Granado Serrano
Ana Belén
AB
Izquierdo-Pulido
María
M
Bravo
Laura
L
Goya
Luis
L
D000975:Antioxidants / Q000494:pharmacology*; D002099:Cacao / Q000737:chemistry*; D002460:Cell Line; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols; D013805:Theobromine / Q000494:pharmacology; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D020122:tert-Butylhydroperoxide; D013805:Theobromine