title: Identification of Curcuma plants and curcumin content level by DNA polymorphisms in the trnS-trnfM intergenic spacer in chloroplast DNA.
abstract: With the goal of developing an accurate plant identification method, molecular analysis based on polymorphisms of the nucleotide sequence of chloroplast DNA (cpDNA) was performed in order to distinguish four Curcuma species: C. longa, C. aromatica, C. zedoaria, and C. xanthorrhiza. Nineteen regions of cpDNA were amplified successfully via polymerase chain reaction (PCR) using total DNA of all Curcuma plants. Using the intergenic spacer between trnS and trnfM (trnSfM), all four Curcuma plant species were correctly identified. In addition, the number of AT repeats in the trnSfM region was predictive of the curcumin content in the rhizome of C. longa.
authors.affiliation: Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan. minami@isc.chubu.ac.jp
authors.forename: Motoyasu
authors.initials: M
authors.lastname: Minami
authors.forename: Kaori
authors.initials: K
authors.lastname: Nishio
authors.forename: Yui
authors.initials: Y
authors.lastname: Ajioka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kyushima
authors.forename: Katsuki
authors.initials: K
authors.lastname: Shigeki
authors.forename: Kaneo
authors.initials: K
authors.lastname: Kinjo
authors.forename: Kazuno
authors.initials: K
authors.lastname: Yamada
authors.forename: Masashi
authors.initials: M
authors.lastname: Nagai
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Satoh
authors.forename: Yozo
authors.initials: Y
authors.lastname: Sakurai
mesh_terms: D002851:Chromatography, High Pressure Liquid; D030024:Curcuma / Q000235:genetics* / Q000378:metabolism*; D003474:Curcumin / Q000378:metabolism*; D018742:DNA, Chloroplast / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D018742:DNA, Chloroplast; D021901:DNA, Intergenic; D012343:RNA, Transfer; D003474:Curcumin