Legume lectin FRIL preserves neural progenitor cells in suspension culture in vitro.
In vitro maintenance of stem cells is crucial for many clinical applications. Stem cell preservation factor FRIL (Flt3 receptor-interacting lectin) is a plant lectin extracted from Dolichos Lablab and has been found preserve hematopoietic stem cells in vitro for a month in our previous studies. To investigate whether FRIL can preserve neural progenitor cells (NPCs), it was supplemented into serum-free suspension culture media. FRIL made NPC grow slowly, induced cell adhesion, and delayed neurospheres formation. However, FRIL did not initiate NPC differentiation according to immunofluorescence and semiquantitive RT-PCR results. In conclusion, FRIL could also preserve neural progenitor cells in vitro by inhibiting both cell proliferation and differentiation.
Yao
Hailei
H
Stem Cells and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
Xie
Xiaoyan
X
Li
Yanhua
Y
Wang
Dongmei
D
Han
Shu
S
Shi
Shuangshuang
S
Nan
Xue
X
Bai
Cixian
C
Wang
Yunfang
Y
Pei
Xuetao
X
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D007887:Fabaceae / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry / Q000235:genetics; D037241:Mannose-Binding Lectins / Q000494:pharmacology*; D037121:Plant Lectins / Q000494:pharmacology*; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D014021:Tissue Preservation
D016895:Culture Media, Serum-Free; C117675:FRIL protein, Dolichos lablab; D037102:Lectins; D037241:Mannose-Binding Lectins; D037121:Plant Lectins