A convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery.
In order to quantify the amount of ligands or poly(ethylene glycol) (PEG) on each vector, here we developed a system in which poly-L-glutamic acid (PLG) was used as surface modification loading backbone, to which one PEG (MW 5000, 10000, 20000) or epidermal growth factor (EGF) was linked. The PLG conjugates can electro-statically adsorb upon DNA/ polycation complex with positive charge, and, the amount of EGF or PEG on the surface of complexes could be varied. We have made a series of complexes containing the various PLG conjugates and examined their physicochemical properties, and made a comparison of properties and transfection efficiency between these complexes. EGF- and PEG-modified complexes showed 10-25-folds higher cell transfection efficiency than unmodified complexes in medium with or without serum.
Sun
Ye
Y
School of Life Science and Technology, Shanghai Jiaotong University, Shanghai, China. sunye01@yahoo.com
Tang
Yuzhao
Y
Chu
Maoquan
M
Song
Shuxian
S
Xin
Yanfei
Y
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage / Q000235:genetics*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D004815:Epidermal Growth Factor / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011099:Polyglutamic Acid / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
D004337:Drug Carriers; D011099:Polyglutamic Acid; D004815:Epidermal Growth Factor; D004247:DNA