title: Analysis of trans-resveratrol in grapes by micro-high performance liquid chromatography.
abstract: Resveratrol, existing in two isomeric trans-and cis-forms, is a phytoalexin that has pharmacological activities, like anti-cancer and anti-cardiovascular diseases as well as life-span increasing properties. Grapes are good sources for resveratrol introduction in the human diet not only directly, but also through its products: wine. In the present work, trans-resveratrol determination and the separation of resveratrol isomers were investigated by the use of micro-high performance liquid chromatography. The analysis conditions were optimized. The baseline separation of trans-and cis-resveratrol was obtained. Further, a trans-resveratrol determination of some grapes growing in Shacheng, Huailai, Hebei province, China was carried out with our analytical method. The concentrations were 6.77 +/- 0.12 and 3.95 +/- 0.18 (microg/g fresh weight) for merlot and cabernet sauvignon grapes, respectively.
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China. fan@pflanzenphys.uni-halle.de
authors.forename: Enguo
authors.initials: E
authors.lastname: Fan
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Sha
authors.initials: S
authors.lastname: Jiang
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Bai
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005843:Geography; D006801:Humans; D007536:Isomerism; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis*; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D000077185:Resveratrol