title: The extracellular proteoglycan produced by Rhodella grisea.
abstract: Highly viscous extracellular proteoglycan (EPG) has been isolated from culture medium of the unicellular red alga Rhodella grisea (Rhodophyceae) by ethanol precipitation. EPG was composed of xylose (29.3%), 3-O-methyl-xylose (26.0%), uronic acids (17.1%), rhamnose (14.4%), galactose (7.5%), glucose (3.9%), arabinose (1.4%) and mannose (0.4%), and traces of fucose, 4-O-methyl-xylose and 2,3-di-O-methyl-rhamnose or fucose. In addition, the polymer contained proteins (13.1%), sulphates and 13C-CP MAS spectra indicated the presence of acetyl and succinyl groups. The molecular mass was estimated to be 136,000. Ion-exchange chromatography afforded five fractions differing in composition of neutral sugars, uronic acids, and protein content indicating thus the complex structure of the EPG.
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia. chemcape@savba.sk
authors.forename: P
authors.initials: P
authors.lastname: Capek
authors.forename: M
authors.initials: M
authors.lastname: Matulová
authors.forename: B
authors.initials: B
authors.lastname: Combourieu
mesh_terms: D005110:Extracellular Space / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011509:Proteoglycans / Q000096:biosynthesis* / Q000737:chemistry; D000461:Rhodophyta / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011509:Proteoglycans