title: eSGA: E. coli synthetic genetic array analysis.
abstract: Physical and functional interactions define the molecular organization of the cell. Genetic interactions, or epistasis, tend to occur between gene products involved in parallel pathways or interlinked biological processes. High-throughput experimental systems to examine genetic interactions on a genome-wide scale have been devised for Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans and Drosophila melanogaster, but have not been reported previously for prokaryotes. Here we describe the development of a quantitative screening procedure for monitoring bacterial genetic interactions based on conjugation of Escherichia coli deletion or hypomorphic strains to create double mutants on a genome-wide scale. The patterns of synthetic sickness and synthetic lethality (aggravating genetic interactions) we observed for certain double mutant combinations provided information about functional relationships and redundancy between pathways and enabled us to group bacterial gene products into functional modules.
authors.affiliation: Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto M5S 3E1, Canada.
authors.forename: Gareth
authors.initials: G
authors.lastname: Butland
authors.forename: Mohan
authors.initials: M
authors.lastname: Babu
authors.forename: J Javier
authors.initials: JJ
authors.lastname: Díaz-Mejía
authors.forename: Fedyshyn
authors.initials: F
authors.lastname: Bohdana
authors.forename: Sadhna
authors.initials: S
authors.lastname: Phanse
authors.forename: Barbara
authors.initials: B
authors.lastname: Gold
authors.forename: Wenhong
authors.initials: W
authors.lastname: Yang
authors.forename: Joyce
authors.initials: J
authors.lastname: Li
authors.forename: Alla G
authors.initials: AG
authors.lastname: Gagarinova
authors.forename: Oxana
authors.initials: O
authors.lastname: Pogoutse
authors.forename: Hirotada
authors.initials: H
authors.lastname: Mori
authors.forename: Barry L
authors.initials: BL
authors.lastname: Wanner
authors.forename: Henry
authors.initials: H
authors.lastname: Lo
authors.forename: Jas
authors.initials: J
authors.lastname: Wasniewski
authors.forename: Constantine
authors.initials: C
authors.lastname: Christopolous
authors.forename: Mehrab
authors.initials: M
authors.lastname: Ali
authors.forename: Pascal
authors.initials: P
authors.lastname: Venn
authors.forename: Anahita
authors.initials: A
authors.lastname: Safavi-Naini
authors.forename: Natalie
authors.initials: N
authors.lastname: Sourour
authors.forename: Simone
authors.initials: S
authors.lastname: Caron
authors.forename: Ja-Yeon
authors.initials: JY
authors.lastname: Choi
authors.forename: Ludovic
authors.initials: L
authors.lastname: Laigle
authors.forename: Anaies
authors.initials: A
authors.lastname: Nazarians-Armavil
authors.forename: Avnish
authors.initials: A
authors.lastname: Deshpande
authors.forename: Sarah
authors.initials: S
authors.lastname: Joe
authors.forename: Kirill A
authors.initials: KA
authors.lastname: Datsenko
authors.forename: Natsuko
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Brenda J
authors.initials: BJ
authors.lastname: Andrews
authors.forename: Charles
authors.initials: C
authors.lastname: Boone
authors.forename: Huiming
authors.initials: H
authors.lastname: Ding
authors.forename: Bilal
authors.initials: B
authors.lastname: Sheikh
authors.forename: Gabriel
authors.initials: G
authors.lastname: Moreno-Hagelseib
authors.forename: Jack F
authors.initials: JF
authors.lastname: Greenblatt
authors.forename: Andrew
authors.initials: A
authors.lastname: Emili
mesh_terms: D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D016680:Genome, Bacterial; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis