The purine machine scores a base hit.
A new synthetic method allows incorporation of 13C or 15N into selected positions within purine nucleotide bases, starting from simple labeled precursors. The procedure harnesses diverse enzymes to support biosynthesis by the pentose phosphate and de novo purine pathways. Selective isotope incorporation should expand the range of RNAs that are amenable to NMR analysis.
Kappock
T Joseph
TJ
Department of Biochemistry, Purdue University, 175 S. University Street, West Lafayette, Indiana 47907, USA. kappock@purdue.edu
D002247:Carbon Isotopes; D004798:Enzymes / Q000737:chemistry; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011685:Purine Nucleotides / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000737:chemistry
D002247:Carbon Isotopes; D004798:Enzymes; D009587:Nitrogen Isotopes; D011685:Purine Nucleotides; D012313:RNA