[RGS1 is involved in the regulation of co-stimulatory molecules and cytokines in RAW264.7 cells].
OBJECTIVE
To investigate the effect of regulator of G protein 1 (RGS1) on the expression of costimulatory molecules and the production of cytokines in RAW264.7 cells.


METHODS
RGS1 was cloned and then used to transfect RAW264.7 cells to set up stably tranfected cell line. The function of RGS1 was detected by flow cytometry (FACS) and global microarray.


RESULTS
RGS1 inhibited the activation of NF-kappaB, significantly promoted the expression of surface molecular CD86, and down-regulated the expression of CD80. Upon the exposure to different kinds of Toll like receptor ligands (TLRL), RGS1 remarkably inhibited the expression of surface molecules CD40, CD80 and PD1. Meanwhile, RGS1 significantly up-regulated the expression of IL-6 and IL-15, down-regulated the expression of IL-10 and IL-1.


CONCLUSIONS
RGS1 can modulate the expression of costimulatory molecules and cytokine secretion, and interrupt Toll like receptor signal pathways, which suggest that RGS1 may play a role in regulating immune responses.
Liu
Yu
Y
Department of Immunology, School of Medicine, Nankai University, Tianjin 300071, China.
Jiao
Guo-Hui
GH
Liu
Bin
B
Chen
Si
S
Zhang
Yuan
Y
Yang
Rong-Cun
RC
D000818:Animals; D018122:B7-1 Antigen / Q000378:metabolism; D002460:Cell Line; D005434:Flow Cytometry; D007375:Interleukin-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D019409:Interleukin-15 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D016328:NF-kappa B / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020710:RGS Proteins / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
D018122:B7-1 Antigen; D007375:Interleukin-1; D019409:Interleukin-15; D015850:Interleukin-6; D016328:NF-kappa B; D020710:RGS Proteins; C488188:Rgs1 protein, mouse; D016753:Interleukin-10