title: Expression, crystallization and preliminary X-ray crystallographic analysis of XometC, a cystathionine gamma-lyase-like protein from Xanthomonas oryzae pv. oryzae.
abstract: Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight of rice (Oryza sativa L.), one of the most devastating diseases of rice in most rice-growing countries. XometC, a cystathionine gamma-lyase (CGL) like protein that is an antibacterial drug-target protein against Xoo, was cloned, expressed, purified and crystallized. CGL catalyzes the second step in the reverse-transsulfuration pathway, which is essential for the metabolic interconversion of the sulfur-containing amino acids cysteine and methionine. Crystals of two different shapes, plate-shaped and pyramid-shaped, diffracted to 2.9 and 3.2 A resolution and belonged to the primitive orthogonal space group P2(1)2(1)2(1) and the tetragonal space group P4(1) (or P4(3)), with unit-cell parameters a = 73.0, b = 144.9, c = 152.3 A and a = b = 78.2, c = 300.7 A, respectively. For the P2(1)2(1)2(1) crystals, three or four monomers exist in the asymmetric unit with a corresponding V(M) of 3.02 or 2.26 A(3) Da(-1) and a solvent content of 59.3 or 45.7%. For the P4(1) (or P4(3)) crystals, four or five monomers exist in the asymmetric unit with a corresponding V(M) of 2.59 or 2.09 A(3) Da(-1) and a solvent content of 52.5 or 40.6%.
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Phuong Thuy Ho
authors.initials: PT
authors.lastname: Ngo
authors.forename: Jin Kwang
authors.initials: JK
authors.lastname: Kim
authors.forename: Hyesoon
authors.initials: H
authors.lastname: Kim
authors.forename: Junho
authors.initials: J
authors.lastname: Jung
authors.forename: Yeh Jin
authors.initials: YJ
authors.lastname: Ahn
authors.forename: Jeong Gu
authors.initials: JG
authors.lastname: Kim
authors.forename: Byoung Moo
authors.initials: BM
authors.lastname: Lee
authors.forename: Hee Wan
authors.initials: HW
authors.lastname: Kang
authors.forename: Lin Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D003542:Cystathionine gamma-Lyase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D011487:Protein Conformation; D014974:Xanthomonas / Q000201:enzymology*
chemical_list: D017931:DNA Primers; D003542:Cystathionine gamma-Lyase