Effects of indomethacin and buprenorphine analgesia on the postoperative recovery of mice.
Buprenorphine (Bup) is the most commonly used analgesic in mice, yet few objective assessments address its superiority for postsurgical recovery. In mice, IP implantation of a radiotelemetry device induces decreases in body weight (BW), food and water intake (FI, WI), core temperature (Tc), and activity levels that persist approximately 14 d in the absence of analgesia. To compare the efficacy of Bup with that of the nonsteroidal antiinflammatory drug indomethacin (Indo) for postsurgical recovery, male C57BL/6J mice were treated on the day of radiotelemetry implantation with Bup (0.3 mg/kg s.c.) or Indo (1 mg/kg s.c.) followed by treatment with Indo (1 mg/kg p.o.) on the next day (Bup-Indo versus Indo-Indo). Responses were compared between treatments in mice implanted with a radiotelemetry device and those that did not undergo surgery. Changes in BW, FI, WI, Tc, and activity were examined throughout 14 d of recovery. Indo-Indo was more efficacious in inhibiting postsurgical BW, FI, and WI reductions, compared with Bup-Indo. Bup also reduced BW and FI in the absence of surgery, indicating a nonspecific effect of this drug on these variables. Indo-Indo treatment was associated with higher activity levels during lights-on-to-lights-off transition periods compared with that observed with Bup-Indo. According to 5 objective measures of surgical recovery, our data suggest that Indo-Indo treatment is more efficacious than is Bup-Indo for postsurgical recovery of radiotelemetry-implanted mice.
Blaha
Michael D
MD
US Army Research Institute of Environmental Medicine, Thermal and Mountain Medicine Division, Natick, MA, USA. michael.blaha@amedd.army.mil
Leon
Lisa R
LR
D000284:Administration, Oral; D000701:Analgesics, Opioid / Q000627:therapeutic use*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D001835:Body Weight / Q000502:physiology; D002047:Buprenorphine / Q000627:therapeutic use*; D004326:Drinking / Q000502:physiology; D004359:Drug Therapy, Combination; D004435:Eating / Q000502:physiology; D007213:Indomethacin / Q000627:therapeutic use*; D007756:Laboratory Animal Science / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology; D010149:Postoperative Pain / Q000503:physiopathology / Q000517:prevention & control*; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology; D013047:Specific Pathogen-Free Organisms; D013686:Telemetry
D000701:Analgesics, Opioid; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002047:Buprenorphine; D007213:Indomethacin