[Freeze-drying of oxygen-carrying substances and storage stability of the freeze-dried solids].
Oxygen-carrying substances based on poly-placental hemoglobin were put into freeze-drying. Sucrose was chosen to inhibit the methmoglobin(MetHb) formation. MetHb content, ultravioletes(UV) spectrum, Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), High performance liquid chromatography (HPLC) were monitored during freeze-drying. As a result, when the mass ratio of sucrose to protein was above 0.5, MetHb formation was under control;the characteristic absorption of UV spectrum, SDS-PAGE and HPLC showed no visible change. Freeze-dried solids were kept under room temperature and refrigerator for 3 months. As a result, MetHb formation depended upon storage temperature and the mass ratio of sucrose to protein. For group C in which the mass ratio of sucrose to protein was 1.0; there is no marked change in MetHb content, UV spectrum, SDS-PAGE and HPLC after 3 months.
Wang
Zhi
Z
West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Li
Fengjuan
F
Zhang
Honghui
H
Yu
Rong
R
Yang
Chengmin
C
D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004356:Drug Storage; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005612:Freeze Drying; D006422:Hemerythrin / Q000032:analysis; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D008706:Methemoglobin / Q000032:analysis*; D010920:Placenta / Q000737:chemistry; D013395:Sucrose / Q000494:pharmacology
D006422:Hemerythrin; D006454:Hemoglobins; C068346:oxyhemerythrin; D013395:Sucrose; D008706:Methemoglobin