Ectomycorrhiza of Kobresia myosuroides at a primary successional glacier forefront.
The bog sedge Kobresia myosuroides is among the first ectomycorrhizal (EM) plants forming dense pads on receding glacier forefronts of the Austrian Alps. This is the only Cyperaceae species known to form EM. To date, little is known about fungal species involved in these EM associations. Therefore, the main aim of this study was to detect EM fungal communities of K. myosuroides (1) by describing mycorrhizal morphotypes (MT) and (2) by identifying the mycobionts by rDNA internal transcribed spacer (ITS) sequencing. Furthermore, seasonal dynamics of Kobresia mycobionts were investigated. Sampling was performed in all four seasons (also under snow cover) during the years 2005 and 2006 at the Rotmoos glacier forefront, a well-characterized alpine primary successional habitat in the Austrian alps (2,300 m above sea level). The degree of EM infection of K. myosuroides roots was high (95%). Ten MTs were described and sequences of 18 fungal taxa were obtained. This was the highest mycobiont diversity ever reported for this plant. Cenococcum geophilum was the most abundant mycobiont (37-46%) and shared dominancy with Sebacina incrustans (16-44%) and Tomentella spp. (7-37%). Tomentella (including Thelephora) was the most species-rich mycobiont genus with five taxa, followed by Cortinarius, Inocybe, and Sebacina with two taxa each and one Hebeloma species. Other ascomycete mycobionts beside C. geophilum were Helvella sp., Lecythophora sp., and one Pezizales species. Due to high interannual differences in the EM fungal community, no significant seasonal changes could be detected. The importance of fungal mycobionts in alpine habitats is discussed.
Mühlmann
Oliver
O
Institute of Microbiology, University Innsbruck, Technikerstr. 25, 6020 Innsbruck, Austria. oliver.muehlmann@uibk.ac.at
Peintner
Ursula
U
D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001317:Austria; D001487:Basidiomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D029785:Cyperaceae / Q000254:growth & development / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D017753:Ecosystem; D046448:Ice Cover / Q000382:microbiology; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000254:growth & development*; D018517:Plant Roots / Q000382:microbiology*; D012621:Seasons; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer