title: Glycine transporters (glycine transporter 1 and glycine transporter 2) are expressed in retina.
abstract: The amino acid glycine is an inhibitory neurotransmitter in the spinal cord, brain stem, and vertebrate retina. The effective synaptic concentrations of glycine are regulated by at least two transporters: glycine transporter 1 and glycine transporter 2. Here, we show retinal expression of glycine transporter 1 by in-situ hybridization and of glycine transporter 2 by reverse transcriptase-PCR and in-situ hybridization. In-situ hybridization signals were observed in the ganglionar and inner nuclear layer as well as in the outer nuclear layer of the frog and rat retinas. In addition, accumulation of H-glycine was observed in isolated photoreceptor cells. The expression of these transporters in nonglycinergic cells suggests that they may also modulate electrical signals.
authors.affiliation: Department of Developmental Neurobiology and Neurophysiology, Institute of Neurobiology, Universidad Nacional Autonoma de Mexico, Mexico.
authors.forename: Ma Teresa
authors.initials: MT
authors.lastname: Peña-Rangel
authors.forename: Juan R
authors.initials: JR
authors.lastname: Riesgo-Escovar
authors.forename: Gustavo
authors.initials: G
authors.lastname: Sánchez-Chávez
authors.forename: Rocío
authors.initials: R
authors.lastname: Salceda
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D050487:Glycine Plasma Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D017403:In Situ Hybridization; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D020419:Photoreceptor Cells, Vertebrate / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011894:Rana pipiens; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D050487:Glycine Plasma Membrane Transport Proteins; D012333:RNA, Messenger; C493269:Slc6a5 protein, rat; C493266:Slc6a9 protein, rat