Plant glycoprotein modulates the expression of interleukin-1beta via inhibition of MAP kinase in HMC-1 cells.
Dioscorea batatas Decne (DBD) is used to heal various disorders of the kidney and lungs as an herbal agent in Korea. The purpose of the present study was to determine whether the DBD glycoprotein regulates the inflammatory reaction stimulated by phorbol-12-myristate 13-acetate plus calcium ionophore A23187 (PMACI) in human mast cells (HMC-1). The results indicate that DBD glycoprotein decreased gene expression of interleukin (IL)-1beta and cyclooxygenase (COX)-2 in PMACI-stimulated HMC-1 cells through blocking of phosphorylation of p44/42 mitogen-activated protein kinase (MAPK) and p38 MAPK and DNA binding activities of nuclear factor (NF)-kappaB and activator protein (AP)-1. The production of intracellular reactive oxygen species (ROS) and nitric oxide (NO) is gradually reduced by concentration-dependent DBD glycoprotein treatment in PMACI-stimulated HMC-1 cells. Hence, we propose the hypothesis that DBD glycoprotein can serve as a potent anti-inflammatory agent in the treatment of inflammatory allergic diseases through inhibition of inflammation-related signal transduction in mast cell activation.
Oh
Phil-Sun
PS
Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, Gwang-ju, South Korea.
Lim
Kye-Taek
KT
D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D027742:Dioscorea / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D053583:Interleukin-1beta / Q000096:biosynthesis*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D006023:Glycoproteins; D053583:Interleukin-1beta; D016328:NF-kappa B; D047428:Protein Kinase Inhibitors; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D000001:Calcimycin; D051546:Cyclooxygenase 2; D020928:Mitogen-Activated Protein Kinases