Zone electrophoresis in an inner-cooling wide-bore electrophoresis system with UV detection.
A novel, high-performance wide-bore electrophoresis (WE) system with inner-cooling has been developed. By introducing the mode of a shell and tube heat exchanger into this system to remove Joule heat generated during electrophoresis, it is feasible to extend electrophoresis from the conventional capillary (i.d. <100 microm) to a wide-bore tube (i.d. >1000 microm). The wide tube allows the loading of over 1.0 microL of the sample with an LOD of 3.0 x 10(-4) mg/mL (signal-to-noise ratio, 3:1). Satisfactory separations of model compounds have been achieved on the WE system.
Guo
Yugao
Y
School of Material Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, P. R. China.
Liu
Danning
D
Wang
Huaifeng
H
Yuan
Ruijuan
R
Bao
James Jianmin
JJ
D019817:Benzoic Acid / Q000032:analysis* / Q000737:chemistry; D003080:Cold Temperature; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006358:Hot Temperature; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D013451:Sulfonic Acids / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry*
D013451:Sulfonic Acids; D014867:Water; D019817:Benzoic Acid