title: Genetic variation in the mannosidase 2B2 gene and its association with ovulation rate in pigs.
abstract: Ovulation rate is an important phenotypic trait that is a critical component of litter size in pigs. Despite being moderately heritable in pigs, selection for increased ovulation rate is difficult because it is difficult to measure and is a sex-limited trait. A QTL for ovulation rate residing on the p-terminal end of pig chromosome 8 has been detected in a Meishan-cross resource population. Comparative analysis of this region yielded a positional candidate gene, mannosidase 2B2 (MAN2B2), for this QTL. The entire coding region of MAN2B2 was resequenced in the Meishan and White Composite founder animals of the resource population to identify SNPs. Eleven polymorphisms that alter the protein product of MAN2B2 were discovered and tested for statistical associations with ovulation rate in three generations of the resource population. The polymorphism located at position 1574 of the mRNA (D28521:c.1574A>G) was the most significant polymorphism tested (P = 0.00005) where the additive effect of the c.1574A allele was estimated to be -0.89 ova. This polymorphism was determined to be more significantly associated with ovulation rate than the breed-specific analysis conducted during the line-cross QTL discovery. The c.1574A>G marker was not associated with ovulation rate in an occidental population. Therefore, either MAN2B2 has a unique epistatic interaction within the Meishan-cross population or the c.1574A>G SNP is in linkage disequilibrium with the actual causative genetic variation in the Meishan-cross population.
authors.affiliation: USDA, ARS, US Meat Animal Research Center, Clay Center, NE 68933-0166, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Campbell
authors.forename: D J
authors.initials: DJ
authors.lastname: Nonneman
authors.forename: L A
authors.initials: LA
authors.lastname: Kuehn
authors.forename: G A
authors.initials: GA
authors.lastname: Rohrer
mesh_terms: D000818:Animals; D005260:Female; D008361:Mannosidases / Q000235:genetics*; D010060:Ovulation; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D013045:Species Specificity; D013552:Swine / Q000235:genetics* / Q000502:physiology
chemical_list: D008361:Mannosidases