title: Microlunatus aurantiacus sp. nov., a novel actinobacterium isolated from a rhizosphere soil sample.
abstract: A Gram-positive, aerobic, non-motile, mesophilic strain, designated YIM 45721T, was isolated from a rhizosphere soil sample in Yunnan Province, China. On the basis of 16S rRNA gene sequence similarity analysis, strain YIM 45721T was assigned to the genus Microlunatus and was most closely related to the type strain of Microlunatus phosphovorus (98.2 % 16S rRNA gene sequence similarity). Chemotaxonomic data, including cell-wall peptidoglycan structure, menaquinones and fatty acid profile, supported affiliation of strain YIM 45721T to the genus Microlunatus. Results of DNA-DNA hybridization and physiological and biochemical tests enabled strain YIM 45721T to be differentiated genotypically and phenotypically from M. phosphovorus. Therefore, strain YIM 45721T represents a novel species of the genus Microlunatus, for which the name Microlunatus aurantiacus sp. nov. is proposed. The type strain is YIM 45721T (=CCTCC AB 206067T=DSM 18424T).
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, PR China.
authors.forename: Yong-Xia
authors.initials: YX
authors.lastname: Wang
authors.forename: Man
authors.initials: M
authors.lastname: Cai
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Zhi
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Cui
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D011423:Propionibacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D020946:Taxus / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S