Characterization of anti-channel catfish MHC class IIbeta monoclonal antibodies.
This study characterizes four monoclonal antibodies (mAb) developed against the major histocompatibility complex (MHC) class II beta chain of the channel catfish, Ictalurus punctatus. Immunoprecipitations using catfish clonal B cells revealed that each of these mAbs immunoselected proteins of approximately 32 and 36 kD, which are of the appropriate sizes for MHC class II alpha and beta chains, respectively. Cell distribution studies using a fluorescence-activated cell sorter (FACS) combined with RT-PCR analyses demonstrated that MHC class II beta is expressed at a high density on catfish clonal macrophage, B and T cell lines, on alloantigen stimulated leukocytes, and on lipopolysaccharide-induced B-cell blasts. Collectively, these results demonstrate the potential importance of these antibodies as reagents in future studies dealing with the functional role of MHC class II molecules in immune recognition of self from non-self.
Moulana
Mohadetheh
M
Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA.
Evenhuis
Jason
J
Albertino
Mark
M
Godwin
Ulla
U
Kountikov
Evgueni I
EI
Stuge
Tor B
TB
Wilson
Melanie
M
Bengtén
Eva
E
Miller
Norman W
NW
McConnell
Thomas J
TJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000276:immunology; D005802:Genes, MHC Class II / Q000276:immunology*; D007059:Ictaluridae / Q000276:immunology*; D047468:Immunoprecipitation
D000911:Antibodies, Monoclonal