Differences and molecular immunohistochemical parameters in the subtypes of infiltrating ductal breast cancer.
Breast cancer is a heterogeneous disease, and patients are categorized into subtypes according to gene expression. We studied the associations among molecular, immunohistochemical, and clinicopathologic features and their distribution according to the subtypes luminal, HER2, basal, and normal-like in 60 patients with invasive ductal breast carcinoma without distant metastasis at the time of diagnosis (M0). We evaluated the hypermethylation of the CDH-1, RASSF1A, SIAH-1 and TSLC-1 genes by methylation-specific polymerase chain reaction and the expression of p53, bcl-2, cyclin D1, E-cadherin, and beta-catenin proteins in tissue microarrays by immunohistochemical analysis. Expression of bcl-2 was associated with the luminal subtype (P=.003), and CDH-1 hypermethylation was present preferentially in HER2 tumors (P=.038). The basal subtype was characterized by the expression of beta-catenin (P=.003). The hypermethylation of CDH-1 and the expression of bcl-2, cyclin D1, and beta-catenin proteins differ among breast cancer subtypes.
Bertolo
Cristina
C
Biomedical Research Center, Department of Surgery, Navarra Health Service, Pamplona, Spain.
Guerrero
David
D
Vicente
Francisco
F
Cordoba
Alicia
A
Esteller
Manel
M
Ropero
Santiago
S
Guillen-Grima
Francisco
F
Martinez-Peñuela
Jose Maria
JM
Lera
Jose Miguel
JM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD; D001943:Breast Neoplasms / Q000473:pathology*; D015820:Cadherins / Q000235:genetics; D018270:Carcinoma, Ductal, Breast / Q000473:pathology*; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D019175:DNA Methylation; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies; D016019:Survival Analysis; D025521:Tumor Suppressor Proteins / Q000235:genetics
D015703:Antigens, CD; C403514:CADM1 protein, human; C513384:CDH1 protein, human; D015820:Cadherins; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D008565:Membrane Proteins; C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins