title: Isolation and characterization of multipotent human keloid-derived mesenchymal-like stem cells.
abstract: In this study, we report the isolation and characterization of a population of multipotent keloid-derived mesenchymal-like stem cells (KMLSCs) from keloid scalp tissues. These KMLSCs expressed the typical mesenchymal stem cell marker proteins CD13, CD29, CD44, CD90, fibronectin, and vimentin when they were cultured in serum-containing medium and when subsequent exposure to various differentiation media resulted in their differentiation into adipocytes, osteoblasts, chondrocytes, smooth muscle cells, and angiogenic endothelial cells. When KMLSCs were cultured in neural stem culture conditions (i.e., in the presence of epidermal growth factor and fibroblast growth factor 2 in substrate-free conditions), they produced large numbers of neurospheres containing nestin-, CD133-, and SOX2-positive cells that expressed neural-crest stem cell markers. Subsequent exposure of these cells to different differentiation conditions resulted in cells that expressed neuronal cell-, astrocyte-, oligodendrocyte-, or Schwann cell-specific markers. Our study suggests that KMLSCs may be an alternative adult stem cell resource for regenerative tissue repair and auto-transplantation.
authors: The Laboratory of Cell Growth and Function Regulation, College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
authors: Jai-Hee
authors: JH
authors: Moon
authors: Sung Sik
authors: SS
authors: Kwak
authors: Gyuman
authors: G
authors: Park
authors: Hye-Youn
authors: HY
authors: Jung
authors: Byung Sun
authors: BS
authors: Yoon
authors: Jaeyeo
authors: J
authors: Park
authors: Kyung Su
authors: KS
authors: Ryu
authors: Seung-Cheol
authors: SC
authors: Choi
authors: Isaac
authors: I
authors: Maeng
authors: Bona
authors: B
authors: Kim
authors: Eun Kyung
authors: EK
authors: Jun
authors: Soonseong
authors: S
authors: Kim
authors: Aeree
authors: A
authors: Kim
authors: Sejong
authors: S
authors: Oh
authors: Hyunggee
authors: H
authors: Kim
authors: Ki Dong
authors: KD
authors: Kim
authors: Seungkwon
authors: S
authors: You
mesh_terms: D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D015703:Antigens, CD / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007627:Keloid; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009417:Nerve Tissue / Q000166:cytology / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology / Q000378:metabolism; D012038:Regeneration; D014182:Transplantation, Autologous
chemical_list: D015703:Antigens, CD; D003470:Culture Media; D016207:Cytokines