Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis.
Glycans have great potential as disease biomarkers and therapeutic targets. However, the major challenge for glycan biomarker identification from clinical samples is the low abundance of key glycosylated proteins. To demonstrate the potential for glycan analysis with nanoliter amounts of glycoprotein, we have developed a new technology (Lectin NanoProbeArray) based on piezoelectric liquid dispensing for non-contact printing and probing of a lectin array. Instead of flooding the glycoprotein probe on the lectin array surface, as in conventional microarray screening, a piezoelectric printer is used to dispense nanoliters of fluorescently labeled glycoprotein probe over the lectin spots on the array. As a proof-of-concept, the ability of Lectin NanoProbeArrays to precisely identify and reliably distinguish between the closely related glycoforms of fetuin is illustrated here. Sensitivity levels comparable to lectin arrays that use evanescent-field scanners was achieved along with several orders of magnitude reduction in the amount of probe required for glycosylation analysis.
Nagaraj
Vinay J
VJ
Center for Applied NanoBioscience, The Biodesign Institute, Arizona State University, 1001 S. McAllister Avenue, P.O. Box 875101, Tempe, AZ 85287, USA. vinay.nagaraj@asu.edu
Eaton
Seron
S
Thirstrup
Derek
D
Wiktor
Peter
P
D015415:Biomarkers / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry; D006023:Glycoproteins / Q000032:analysis*; D006031:Glycosylation; D037102:Lectins / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011134:Polysaccharides / Q000032:analysis*; D011327:Printing
D015415:Biomarkers; D005456:Fluorescent Dyes; D006023:Glycoproteins; D037102:Lectins; D011134:Polysaccharides