title: Efficient delivery of human single fiber-derived muscle precursor cells via biocompatible scaffold.
abstract: The success of cell therapy for skeletal muscle disorders depends upon two main factors: the cell source and the method of delivery. In this work we have explored the therapeutic potential of human muscle precursor cells (hMPCs), obtained from single human muscle fibers, implanted in vivo via micropatterned scaffolds. hMPCs were initially expanded and characterized in vitro by immunostaining and flow cytometric analysis. For in vivo studies, hMPCs were seeded onto micropatterned poly-lactic-glycolic acid 3D-scaffolds fabricated using soft-lithography and thermal membrane lamination. Seeded scaffolds were then implanted in predamaged tibialis anterior muscles of CD1 nude mice; hMPCs were also directly injected in contralateral limbs as controls. Similarly to what we previously described with mouse precursors cells, we found that hMPCs were able to participate in muscle regeneration and scaffold-implanted muscles contained a greater number of human nuclei, as revealed by immunostaining and Western blot analyses. These results indicate that hMPCs derived from single fibers could be a good and reliable cell source for the design of therapeutic protocols and that implantation of cellularized scaffolds is superior to direct injection for the delivery of myogenic cells into regenerating skeletal muscle.
authors: Department of Paediatrics, University of Padova, Padova, Italy.
authors: Luisa
authors: L
authors: Boldrin
authors: Alberto
authors: A
authors: Malerba
authors: Libero
authors: L
authors: Vitiello
authors: Elisa
authors: E
authors: Cimetta
authors: Martina
authors: M
authors: Piccoli
authors: Chiara
authors: C
authors: Messina
authors: Pier Giorgio
authors: PG
authors: Gamba
authors: Nicola
authors: N
authors: Elvassore
authors: Paolo
authors: P
authors: De Coppi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D048091:Guided Tissue Regeneration / Q000379:methods; D006801:Humans; D019344:Lactic Acid; D051379:Mice; D032342:Muscle Cells / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D032446:Myoblasts / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D011091:Polyesters; D011108:Polymers; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)