New staining method for use in electrophoretic determination of alpha-amylase isoenzymes in serum.
We have developed and evaluated a new staining method for use in electrophoretic analysis of alpha-amylase (AMY, EC3. 2. 1. 1) isoenzymes in serum. Our method based on the colorimetric determination of formazane dye formed by enzyme-coupled reactions and maltopentaose was used as a substrate. We have confirmed that this method was sensitive and stable. Liquid reagents are used, so the automatic analysis of AMY isoenzymes using a fully-automated electrophoresis system will become possible.
Watanabe
Shin-ichiro
S
Department of Clinical Laboratory, Yokohama City University Hospital, Yokohama.
Ogawa
Noboru
N
Kawada
Kaoru
K
Yaba
Keiko
K
Konno
Minoru
M
D001322:Autoanalysis / Q000379:methods*; D001797:Blood Protein Electrophoresis / Q000379:methods*; D003124:Colorimetry; D005562:Formazans; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D009844:Oligosaccharides; D013194:Staining and Labeling / Q000379:methods*; D000516:alpha-Amylases / Q000097:blood*
D005562:Formazans; D007527:Isoenzymes; D009844:Oligosaccharides; C019193:maltopentaose; D000516:alpha-Amylases