Effect of Terminalia arjuna extract on adriamycin-induced DNA damage.
The effect of a bark extract of Terminalia arjuna (TAE) was studied on the alteration of adriamycin (ADR)-induced micronuclei formation in cultured human peripheral blood lymphocytes. Exposure of lymphocytes to ADR resulted in a dose-dependent increase in the micronuclei formation indicating DNA damage. Pretreatment of lymphocytes with TAE before ADR treatment resulted in a significant decline in micronuclei formation. Increasing doses of ADR caused a dose-dependent increase in the frequency of lymphocytes bearing one, two and multiple micronuclei. Prior exposure of lymphocytes to 15 microg/mL of TAE significantly reduced the frequency of lymphocytes bearing one, two and multiple micronuclei when compared with ADR-treated control. TAE-inhibited the induction of (*)OH (hydroxyl), O2(*-) (superoxide), DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS(*+) (2,2-azino-bis-3-ethyl benzothiazoline-6-sulphonic acid) radicals in a dose-dependent manner. These results demonstrate that TAE protects DNA against ADR-induced damage.
Reddy
Tiyyagura Koti
TK
Division of Cancer Biology, Sugen Life Sciences, Tirupati, 517505, AP, India. drkotireddy@gmail.com
Seshadri
Prabhu
P
Reddy
K Kodanda Rami
KK
Jagetia
G C
GC
Reddy
C Damodar
CD
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D015162:Micronucleus Tests; D010936:Plant Extracts / Q000494:pharmacology*; D029766:Terminalia / Q000737:chemistry*
D000970:Antineoplastic Agents; D016166:Free Radical Scavengers; D010936:Plant Extracts; D004317:Doxorubicin