title: Process research on the preparation of DMT protected 2'-O-methoxyethylguanosine for oligonucleotide synthesis in therapeutic applications.
abstract: An optimized process to synthesize DMT protected 2 '-O-methoxyethylguanosine is described. A key step involves the enzymatic deamination of a mixture of alkylated products to selectively afford the desired material without resorting to chromatography for purification. This approach was scaled up to kilogram quantities for use in oligonucleotide therapeutics. [Formula: see text].
authors: Isis Pharmaceuticals, Inc., Carlsbad, California, USA.
authors: Shabbir Ali S
authors: SA
authors: Taj
authors: S
authors: S
authors: Narayanan
authors: S Suman
authors: SS
authors: Meenakshi
authors: Yogesh S
authors: YS
authors: Sanghvi
authors: Bruce S
authors: BS
authors: Ross
authors: Vasulinga T
authors: VT
authors: Ravikumar
mesh_terms: D000243:Adenosine Deaminase / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005786:Gene Expression Regulation / Q000235:genetics*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D013058:Mass Spectrometry; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000235:genetics
chemical_list: C484916:2'-O-methoxyethylguanosine; D009841:Oligonucleotides; D006151:Guanosine; D000243:Adenosine Deaminase