Nonlinear immunofluorescent assay for androgenic hormones based on resonant structures.
We report for the first time the use of two photon fluorescence as detection method of affinity binding reactions. We use a resonant grating waveguide structure as platform enhancement for detecting the interaction between fluorescent labeled Boldenone, a non-natural androgenic hormone, and a specific anti-anabolic antibody. We were able to detect a surface coverage of approximately 0.7 ng/mm(2).
Thayil
Anisha
A
ICFO-Institut de Ciencies Fotoniques, Ada Canal Olimpic s/n, 08600 Castelldefels, Barcelona, Spain.
Muriano
Alejandro
A
Salvador
J-Pablo
JP
Galve
Roger
R
Marco
Maria P
MP
Zalvidea
Dobryna
D
Loza-Alvarez
Pablo
P
Katchalski
Tsvi
T
Grinvald
Eran
E
Friesem
Asher A
AA
Soria
Silvia
S
D000728:Androgens / Q000032:analysis*; D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D017711:Nonlinear Dynamics; D010783:Photometry / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D014159:Transducers
D000728:Androgens