title: A thermodynamic model for Nap1-histone interactions.
abstract: The yeast nucleosome assembly protein 1 (yNap1) plays a role in chromatin maintenance by facilitating histone exchange as well as nucleosome assembly and disassembly. It has been suggested that yNap1 carries out these functions by regulating the concentration of free histones. Therefore, a quantitative understanding of yNap1-histone interactions also provides information on the thermodynamics of chromatin. We have developed quantitative methods to study the affinity of yNap1 for histones. We show that yNap1 binds H2A/H2B and H3/H4 histone complexes with low nm affinity, and that each yNap1 dimer binds two histone fold dimers. The yNap1 tails contribute synergistically to histone binding while the histone tails have a slightly repressive effect on binding. The (H3/H4)(2) tetramer binds DNA with higher affinity than it binds yNap1.
authors: Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523-1870, USA.
authors: Andrew J
authors: AJ
authors: Andrews
authors: Gregory
authors: G
authors: Downing
authors: Kitty
authors: K
authors: Brown
authors: Young-Jun
authors: YJ
authors: Park
authors: Karolin
authors: K
authors: Luger
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000502:physiology*; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D019281:Dimerization; D015966:Gene Expression Regulation, Fungal; D006657:Histones / Q000737:chemistry*; D007700:Kinetics; D008954:Models, Biological; D009687:Nuclear Proteins / Q000737:chemistry / Q000502:physiology*; D056490:Nucleosome Assembly Protein 1; D011485:Protein Binding; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013816:Thermodynamics; D014982:Xenopus laevis
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D006657:Histones; C533556:NAP1 protein, S cerevisiae; D009687:Nuclear Proteins; D056490:Nucleosome Assembly Protein 1; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA