Differential effects of temperature on natural transformation to erythromycin and nalidixic acid resistance in Campylobacter coli.
Campylobacter jejuni and Campylobacter coli are naturally competent, but limited information exists on the impact of environmental conditions on transformation. In this study, we investigated the impact of temperature and microaerobic versus aerobic atmosphere on transformation of C. coli to erythromycin and nalidixic acid resistance. Frequency of transformation was not significantly different between microaerobic (5 to 10% CO(2)) and aerobic conditions. However, C. coli was transformed to erythromycin resistance at a significantly higher frequency at 42 degrees C than at 25 degrees C (P < 0.05), and few or no transformants were obtained at 25 degrees C. In contrast, transformation to nalidixic acid resistance was highly efficient at both 42 degrees C and 25 degrees C and was similar or, at the most, fourfold higher at 42 degrees C than at 25 degrees C. DNase I treatment experiments suggested that steps both prior and subsequent to internalization of DNA were influenced by temperature in the case of transformation of C. coli to erythromycin resistance. However, the moderately increased (fourfold) frequency of transformation to nalidixic acid resistance at 42 degrees C compared to that at 25 degrees C was exclusively associated with steps prior to DNA internalization. These findings suggest that transformation to erythromycin resistance may be significantly more frequent in the gastrointestinal tract of hosts such as poultry (at 42 degrees C) than in other habitats characterized by lower temperatures, whereas transformation to nalidixic acid resistance may be highly efficient both within and outside the animal hosts.
Kim
Joo-Sung
JS
North Carolina State University, Department of Food Science, Raleigh, NC 27695, USA.
Kim
Jae-Won
JW
Kathariou
S
S
D000332:Aerobiosis; D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017000:Campylobacter coli / Q000187:drug effects* / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D004917:Erythromycin / Q000494:pharmacology*; D009268:Nalidixic Acid / Q000494:pharmacology*; D013696:Temperature; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D009268:Nalidixic Acid; D004917:Erythromycin; D003850:Deoxyribonuclease I