Screening of Taxol-producing fungi based on PCR amplification from Taxus.
Genes coding for 10-deacetylbaccatin III-10-O-acetyl transferase and C-13 phenylpropanoid side chain-CoA acyltransferase were used as molecular markers for screening of Taxol-producing endophytic fungi. Using PCR, three out of 90 endophytic fungi, isolated from Taxus x media and Taxus yunnanensis, gave positive results. These 3 strains, when grown in 300 ml potato/dextrose liquid medium at 25 degrees C for 10 days, contained 100-160 microg Taxol/g dry wt of mycelium.
Zhang
Peng
P
Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.
Zhou
Peng-Peng
PP
Jiang
Chen
C
Yu
Hui
H
Yu
Long-Jiang
LJ
D002851:Chromatography, High Pressure Liquid; D005658:Fungi / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017239:Paclitaxel / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D020946:Taxus / Q000382:microbiology*
D017239:Paclitaxel