[Phage resistance of Corynebacterium crenatum conferred by the restriction and modification system cglI].
In order to prevent phage contamination in amino acid fermentation, we introduced the restriction and modification system cglI gene complex into Corynebacterium crenatum and studied their phage-resistance. The cglI gene complex was amplified from Corynebacterium glutamicum by PCR and constructed into pJL23 vector. The recombinant strains were obtained by transformation of the recombinant plasmid pJL23-cglI into C. crenatum. Results showed that the recombinant strains possessed strong phage-resistance activity and broad phage-resistance spectrum, demonstrating the feasibility of using cglI gene complex for construction of phage-resistance recombinant C. crenatum strains and presenting a powerful way to solve the problem of phage contamination in amino acid fermentation industry.
Hu
Yongfei
Y
College of Life Science, Liaoning University, Shenyang 110036, China.
Li
Tiemin
T
Yang
Zhiyong
Z
Zhang
Bo
B
Li
Yu
Y
D000596:Amino Acids / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics*; D001435:Bacteriophages / Q000254:growth & development*; D003352:Corynebacterium / Q000235:genetics* / Q000821:virology; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics*; D005285:Fermentation; D037161:Galectins / Q000235:genetics; D011995:Recombination, Genetic; D014169:Transformation, Bacterial
D000596:Amino Acids; D001426:Bacterial Proteins; D015280:DNA Restriction-Modification Enzymes; D037161:Galectins