Identification of DNA-reactive B cells in patients with systemic lupus erythematosus.
Autoreactive B cells play a central role in systemic lupus erythematosus (SLE). Characterization of DNA-reactive B cells in the blood of lupus patients has been limited by the low frequency of the population. Using a tetrameric configuration of a peptide mimetope of DNA, we identified peptide-reactive B cells in peripheral blood. Antibodies derived from these B cells bound to peptide and were largely cross-reactive to dsDNA. This methodology enables us to track the development of autoreactive B cells, which recognize peptide and dsDNA, in individual patients with SLE and permits the isolation of autoreactive B cells for further characterization.
Zhang
Jie
J
Feinstein Institute for Medical Research, North Shore-Long Island Jewish Health System, Manhasset, New York 11030, USA.
Jacobi
Annett M
AM
Mackay
Meggan
M
Aranow
Cynthia
C
Wang
Tao
T
Chinnasamy
Prameladevi
P
Diamond
Betty
B
D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*
D004247:DNA