Carcinogen adsorbent prepared from DNA complex by gamma-ray irradiation.
The effects of gamma-ray irradiation on aqueous solutions of chub mackerel chromatin, salmon milt DNA with CoCl(2), mixtures of DNA with Type A gelatin, bovine serum albumin (BSA), CM-chitosan, carboxymethyl cellulose (CMC) and Catinal (hydroxyethyl-cellulose, O-[2-hydroxy-3-(trimethyl ammonio)-propyl], chloride) and DNA in the presence of polyfunctional monomers with the aim to insolubilize DNA for preparing a novel carcinogen adsorbent have been studied. Among those, precipitates or inhomogeneous gel consisting of cross-linked DNA were prepared from the samples of aqueous DNA in the presence of CoCl(2) at low irradiation dose, around 10 Gy, and bulk homogeneous gels were successfully prepared from aqueous mixtures of DNA with gelatin, BSA, CMC and Catinal in a limited range of irradiation doses. Gel fraction and swelling ratio of the gels were measured. Adsorption of a carcinogen, acridine orange, was also examined for the gels. From the experimental results, the optimum conditions for preparing insolubilized homogeneous DNA gels were determined.
Furusawa
Kazuya
K
Department of Chemistry and Chemical Biology, Faculty of Engineering, Gunma University, Kiryu, Gunma, Japan.
Kita
Erika
E
Saheki
Toshihiko
T
Nagasawa
Naotsugu
N
Nishi
Norio
N
Dobashi
Toshiaki
T
D000165:Acridine Orange / Q000737:chemistry; D000818:Animals; D002273:Carcinogens / Q000737:chemistry*; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005456:Fluorescent Dyes / Q000737:chemistry; D005720:Gamma Rays; D005782:Gels / Q000737:chemistry / Q000528:radiation effects
D002273:Carcinogens; D005456:Fluorescent Dyes; D005782:Gels; D004247:DNA; D000165:Acridine Orange