Cold acclimation induces physiological cardiac hypertrophy and increases assimilation of triacylglycerol metabolism through lipoprotein lipase.
The contribution of triacylglycerol to energy provision in the hypertrophied heart, mediated through lipoprotein lipase (LPL) is largely unknown and the contribution of very-low-density lipoprotein (VLDL) receptor to control of LPL presentation at the endothelium is unclear. For isolated perfused rat hearts, cold acclimation (CA) induced volume-overload hypertrophy, with decreased developed pressure (P<0.01), increased end-diastolic volume of the left ventricle (P<0.001) and a loss of contractile reserve in response to dobutamine challenge (P<0.01). Oleate utilisation by perfused hearts was unchanged by CA, however uptake of intralipid emulsion increased 3-fold (P<0.01). CA increased the proportion of lipid deposited in tissue lipids from 10% in euthermic controls to 40% (P<0.01) although the overall contribution of individual lipid classes was unaffected. Cold acclimation significantly increased heparin-releasable LPL (P<0.05) and tissue residual LPL (P<0.01). Western blot analysis indicated preserved expression of proteins coding for SERCA2, muscle-CPT1 and VLDL-receptor following CA, while AMPKalpha2 and phospho-AMPKalpha2 were unaffected. These observations indicate that for physiological hypertrophy AMPK phosphorylation does not mediate the enhanced translocation of LPL to cardiac endothelium.
Cheng
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Y
Department of Physiology, Division of Medical Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Hauton
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D
D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000064:Acclimatization / Q000502:physiology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D006332:Cardiomegaly / Q000378:metabolism*; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism; D003080:Cold Temperature; D004075:Diglycerides / Q000378:metabolism; D004280:Dobutamine / Q000494:pharmacology; D005217:Fat Emulsions, Intravenous / Q000378:metabolism; D006321:Heart / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D011973:Receptors, LDL / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
C506948:Atp2a2 protein, rat; D002788:Cholesterol Esters; D004075:Diglycerides; D005217:Fat Emulsions, Intravenous; D011973:Receptors, LDL; D014280:Triglycerides; C036053:VLDL receptor; D019301:Oleic Acid; D004280:Dobutamine; D002334:Carnitine O-Palmitoyltransferase; D055372:AMP-Activated Protein Kinases; D008071:Lipoprotein Lipase; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases