Predominant localization of EFA6A, a guanine nucleotide exchange factor for ARF6, at the perisynaptic photoreceptor processes.
EFA6A is a guanine nucleotide exchange factor that is highly expressed in the nervous system with the ability to activate ADP ribosylation factor 6 (ARF6). In this study, we demonstrated the immunohistochemical localization of EFA6A in the adult mouse retina. Strong immunoreactivity for EFA6A was detected predominantly in the outer plexiform layer (OPL), where EFA6A was partially overlapped with dystrophin and synaptophysin. Immunoelectron microscopic analysis revealed that EFA6A was localized predominantly at the perisynaptic processes of photoreceptor terminals without association with synaptic ribbons. These findings suggest that EFA6A-ARF6 pathway may play a specific role at a subcompartment of perisynaptic photoreceptor processes.
Katsumata
Osamu
O
Department of Anatomy, Kitasato University School of Medicine, Sagamihara 228-8555, Japan.
Honma
Takashi
T
Sanda
Masashi
M
Kamata
Akifumi
A
Takeda
Shin'ichi
S
Kondo
Hisatake
H
Sakagami
Hiroyuki
H
D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D000818:Animals; D016189:Dystrophin / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D016253:Microscopy, Immunoelectron; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism* / Q000648:ultrastructure; D012160:Retina / Q000378:metabolism / Q000648:ultrastructure; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D016708:Synaptophysin / Q000378:metabolism
D000089982:ADP-Ribosylation Factor 6; D016189:Dystrophin; D020662:Guanine Nucleotide Exchange Factors; D006150:Guanine Nucleotides; C504474:Psd protein, mouse; D016708:Synaptophysin; D020727:ADP-Ribosylation Factors; C000715727:Arf6 protein, mouse