Diffuse reflectance spectroscopy with a self-calibrating fiber optic probe.
Calibration of the diffuse reflectance spectrum for instrument response and time-dependent fluctuation as well as interdevice variations is complicated, time consuming, and potentially inaccurate. We describe a novel fiber optic probe with a real-time self-calibration capability that can be used for tissue optical spectroscopy. The probe was tested in a number of liquid phantoms over a relevant range of tissue optical properties. Absorption and scattering coefficients are extracted with an average absolute error and standard deviation of 6.9%+/-7.2% and 3.5%+/-1.5%, respectively.
Yu
Bing
B
Department of Biomedical Engineering, Duke University, Durham, NC 27705, USA. bing.yu@duke.edu
Fu
Henry
H
Bydlon
Torre
T
Bender
Janelle E
JE
Ramanujam
Nirmala
N
D002138:Calibration; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods*; D009010:Monte Carlo Method; D055100:Optical Fibers; D055095:Optics and Photonics; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods*; D014466:Ultraviolet Rays