title: Role of the multidomain protein spinophilin in blood pressure and cardiac function regulation.
abstract: Spinophilin controls intensity/duration of G protein-coupled receptor signaling and thereby influences synaptic activity. We hypothesize that spinophilin affects blood pressure through central mechanisms. We measured blood pressure and heart rate in SPL-deficient (SPL(-/-)), heterozygous SPL-deficient (SPL(+/-)), and wild-type (SPL(+/+)) mice by telemetry combined with fast Fourier transformation. We also assessed peripheral vascular reactivity and performed echocardiography. SPL(-/-) had higher mean arterial pressure than SPL(+/-) and SPL(+/+) (121+/-2, 112+/-1, and 113+/-1 mm Hg). Heart rate was inversely related to spinophilin expression (SPL(-/-) 565+/-0.4, SPL(+/-) 541+/-5, SPL(+/+) 525+/-8 bpm). The blood pressure response to prazosin, trimethapane, and the heart rate response to metoprolol were stronger in SPL(-/-) than SPL(+/+) mice, whereas heart rate response to atropine was attenuated in SPL(-/-). Mesenteric artery vasoreactivity after angiotensin II, phenylephrine, and the thromboxane mimetic (U46619) as well as change in heart rate, stroke volume, and cardiac output after dobutamine were similar in SPL(-/-) and SPL(+/+). Baroreflex sensitivity was attenuated in SPL(-/-) compared with SPL(+/-) and SPL(+/+), which was confirmed by pharmacological testing. Heart rate variability parameters were attenuated in SPL(-/-) mice. We suggest that an increase in central sympathetic outflow participates in blood pressure and heart rate increases in SPL(-/-) mice. The elevated blood pressure in SPL(-/-) mice was associated with attenuated baroreflex sensitivity and decreased parasympathetic activity. Our study is the first to show a role for the spinophilin gene in blood pressure regulation.
authors: Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors: Andrey C
authors: AC
authors: da Costa-Goncalves
authors: Jens
authors: J
authors: Tank
authors: Ralph
authors: R
authors: Plehm
authors: Andre
authors: A
authors: Diedrich
authors: Mihail
authors: M
authors: Todiras
authors: Maik
authors: M
authors: Gollasch
authors: Arnd
authors: A
authors: Heuser
authors: Maren
authors: M
authors: Wellner
authors: Michael
authors: M
authors: Bader
authors: Jens
authors: J
authors: Jordan
authors: Friedrich C
authors: FC
authors: Luft
authors: Volkmar
authors: V
authors: Gross
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects / Q000502:physiology; D017704:Baroreflex / Q000187:drug effects / Q000502:physiology; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D025401:Echocardiography, Stress; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology*; D006352:Heart Ventricles / Q000000981:diagnostic imaging / Q000294:innervation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D015398:Signal Transduction; D014661:Vasoconstriction / Q000187:drug effects; D016276:Ventricular Function; D000099273:Neurabins
chemical_list: D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D010656:Phenylephrine; D011224:Prazosin; D000099273:Neurabins