title: Semi-permeable nanocapsules of konjac glucomannan-chitosan for enzyme immobilization.
abstract: Carboxymethyl konjac glucomannan-chitosan (CKGM-CS) nanocapsules, spontaneously prepared under very mild conditions by electrostatic complexation, were used for immobilizing L-asparaginase. The matrix has semi-permeability to allow the substrate and product to pass through and to keep L-asparaginase in the matrix to prevent leaking. The cell-like hydrogel matrix was prepared in aqueous system without organic solvents and reagents. The process of the preparation does not denature the enzyme and the activity of the immobilized and native enzyme is very similar. The activity, stability, and characters of the enzyme-loaded nanocapsules were studied. The results indicated the immobilized enzyme has better stability and activity in contrast to the native enzyme. These studies may supply a new material for the immobilization of pH and temperature-sensitive enzyme.
authors: Natural Products Research Centre, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors: Rui
authors: R
authors: Wang
authors: Bing
authors: B
authors: Xia
authors: Bang-Jing
authors: BJ
authors: Li
authors: Shu-Lin
authors: SL
authors: Peng
authors: Li-Sheng
authors: LS
authors: Ding
authors: Sheng
authors: S
authors: Zhang
mesh_terms: D031056:Amorphophallus / Q000737:chemistry*; D001215:Asparaginase / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D004339:Drug Compounding; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008351:Mannans / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053769:Nanocapsules; D010316:Particle Size; D010539:Permeability; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D008351:Mannans; D053769:Nanocapsules; C022901:(1-6)-alpha-glucomannan; D048271:Chitosan; D001215:Asparaginase