[Isolation and physiological characteristics of lytic bacteriophages of Vibrio].
OBJECTIVE
Vibrio is a widely distributed pathogen in aquatic environment. Our study aimed at searching for possible biological control of pathogenic vibrio.


METHODS
We collected natural samples from coast and lakes in spring of 2006 and autumn of 2005; and isolated lytic phages by double-layer plate method. We identified the hosts with 16S rDNA sequencing and observed their morphology with phages under electron microscopy. We also tested the physiological characteristics of phages.


RESULTS
We isolated 96 bacteria and 2 phages (Vibio/XM/P1, Vibio/XM/P2). Their hosts belonged to Vibrio alginolyticus and Vibrio anguillarum. Both phages were hexagonal-headed and one with a tail. Physiological tests show that their optimum grow condition were pH7, 25 degrees C and pH8, 30 degrees C. Both phages were sensitive to high temperature and UV light. Vibrio/XM/P2 was sensitive to aether and chloroform whereas Vibrio/XM/P1 not.
Sun
Jia
J
Third Institute of Oceanography, Xiamen 361005, China. oscar_0406@163.com
Ye
Dezan
D
Kochel
Agnes
A
Jost
Guenter
G
D001435:Bacteriophages / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity* / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002725:Chloroform / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004987:Ethers / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D013696:Temperature; D014466:Ultraviolet Rays / Q000009:adverse effects; D014733:Vibrio / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure / Q000821:virology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004987:Ethers; D002725:Chloroform