title: Stress regulated members of the plant organic cation transporter family are localized to the vacuolar membrane.
abstract: BACKGROUND
  In Arabidopsis six genes group into the gene family of the organic cation transporters (OCTs). In animals the members of the OCT-family are mostly characterized as polyspecific transporters involved in the homeostasis of solutes, the transport of monoamine neurotransmitters and the transport of choline and carnitine. In plants little is known about function, localisation and regulation of this gene family. Only one protein has been characterized as a carnitine transporter at the plasma membrane so far.
  
  
  RESULTS
  We localized the five uncharacterized members of the Arabidopsis OCT family, designated OCT2-OCT6, via GFP fusions and protoplast transformation to the tonoplast. Expression analysis with RNA Gel Blots showed a distinct, organ-specific expression pattern of the individual genes. With reporter gene fusion of four members we analyzed the tissue specific distribution of OCT2, 3, 4, and 6. In experiments with salt, drought and cold stress, we could show that AtOCT4, 5 and 6 are up-regulated during drought stress, AtOCT3 and 5 during cold stress and AtOCT 5 and 6 during salt stress treatments.
  
  
  CONCLUSIONS
  Localisation of the proteins at the tonoplast and regulation of the gene expression under stress conditions suggests a specific role for the transporters in plant adaptation to environmental stress.
authors: Center for Plant Molecular Biology-Plant Physiology, Auf der Morgenstelle 1, 72076 Tübingen, Germany. isabell.kuefner@zmbp.uni-tuebingen.de
authors: Isabell
authors: I
authors: Küfner
authors: Wolfgang
authors: W
authors: Koch