Generic nitric oxide (NO) generating surface by immobilizing organoselenium species via layer-by-layer assembly.
A universal nitric oxide (NO) generating surface is assembled via Layer-by-Layer (LbL) deposition of sodium alginate (Alg) and organoselenium modified polyethyleneimine (SePEI) on quartz and polymeric substrates. The immobilized SePEI species is capable of catalytically decomposing S-nitrosothiol species (RSNO) to NO in the presence of thiol reducing agents (e.g., glutathione, cysteine, etc.). The stepwise buildup of the multilayer films is monitored by UV-vis spectroscopy, SEM and surface contact angle measurements. X-ray photoelectron spectroscopy is used to study the stoichiometry between the polyanion and polycation, and also the presence of Se in the catalytic LbL film. A reductive annealing process is necessary to improve the stability of freshly coated multilayer films via chain rearrangement. Chemiluminescence measurements illustrate the ability of the LbL films to generate NO from S-nitrosoglutathione (GSNO) in the presence of glutathione (GSH). Enhanced NO fluxes can be achieved by increasing the number of catalytic (SePEI/Alg) bilayers coated on the substrates. Nitric oxide generation is observed even after prolonged contact with sheep whole blood. Preliminary applications of this LbL on silicone rubber tubings and polyurethane catheters reveal similar NO generation behavior from these biomedical grade polymeric substrates.
Yang
Jun
J
Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109-1055, USA.
Welby
Jenna L
JL
Meyerhoff
Mark E
ME
D000464:Alginates / Q000737:chemistry; D002384:Catalysis; D016650:Fluorescein-5-isothiocyanate; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D009569:Nitric Oxide / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D011791:Quartz; D012643:Selenium / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties
D000464:Alginates; D006603:Hexuronic Acids; D008051:Lipid Bilayers; D011108:Polymers; D011140:Polyurethanes; D013438:Sulfhydryl Compounds; D011791:Quartz; D009569:Nitric Oxide; D020723:Glucuronic Acid; D012643:Selenium; D016650:Fluorescein-5-isothiocyanate; D009584:Nitrogen