Analysis of nucleotide sequence of wheat yellow mosaic virus genomic RNAs.
Wheat yellow mosaic virus (WYMV) isolate HC was used for viral cDNA synthesis and sequencing. The results show that the viral RNAl is 7 629 nucleotides encoding a polyprotein with 2 407 amino acids, from which seven putative proteins may be produced by an autolytic cleavage processing besides the viral coat protein. The RNA2 is 3 639 nucleotides and codes for a polyprotein of 903 amino acids, which may contain two putative non-structural proteins. Although WYMV shares a similarity in genetic organization to wheat spindle streak mosaic virus (WSSMV), the identities in their nucleotide sequences or deduced amino acid sequences are as low as 70 % and 75 % respectively. Based on this result, it is confirmed that WYMV and WSSMV are different species withinBymmirus.
Yu
J
J
State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China.
Yan
L
L
Su
N
N
Hou
Z
Z
Li
D
D
Han
C
C
Yang
L
L
Cai
Z
Z
Liu
Y
Y