Macroporous ordered titanium dioxide (TiO2) inverse opal as a new label-free immunosensor.
Photonic crystal sensing materials have been validated that they are very sensitive to refractive index changes. Herein, three-dimensionally ordered macroporous (3DOM) (>50 nm) TiO2 inverse opal film has been fabricated by the self-assembly technique. Based on the TiO2 inverse opal film, the optical spectrometer was established for label-free immunosensor. The sensing performance of the 3DOM TiO2 was investigated using human IgG/goat anti-human IgG couple, which showed that the sensitivity of 3DOM TiO2 inverse opal film could reach to 1 microg mL(-1) (equivalent to 1.5 pg mm(-2)) of protein concentration detection limit. The 3DOM TiO2 inverse opal has a large internal surface area, low fluorescence background and unique optical properties. These characteristics indicated the feasibility of 3DOM TiO2 inverse opal in label-free immunoassay.
Li
Jianlin
J
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
Zhao
Xiangwei
X
Wei
Hongmei
H
Gu
Zhong-Ze
ZZ
Lu
Zuhong
Z
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D006041:Goats / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D055095:Optics and Photonics; D016062:Porosity; D011485:Protein Binding; D013194:Staining and Labeling; D013379:Substrate Specificity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; C048844:anti-IgG; C009495:titanium dioxide; D014025:Titanium