Retracted: An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86.
A thermophile, halotolerant and organic-solvent-tolerant esterase producer Bacillus sp. S-86 strain previously isolated was found to belong to Bacillus licheniformis species through morphological, biochemical, 16S rRNA gene sequence analyses and rDNA intergenic spacers amplification (ITS-PCR). The strain can grow at 55 degrees C in presence of C2-C7 alkanols (log P=-0.86 to 2.39), and NaCl concentrations up to 15% (w/v). This bacterium showed optimal growth and esterase production at 50 degrees C. Two different molecular weight esterase activities were detected in zymographic assays. PMSF inhibited type I esterase activity, showing no inhibitory effect on type II esterase activity. B. licheniformis S-86 was able to grow in presence of hydroxylic organic-solvents like propan-2-ol, butan-1-ol and 3-methylbutan-1-ol. At a sub-lethal concentration of these solvents (392 mmoll(-1) propan-2-ol; 99 mmol l(-1) butan-1-ol, 37 mmol l(-1) 3-methylbutan-1-ol), adequate to produce 50% cell growth inhibition at 50 degrees C, an increment between 1.9 and 2.3 times was observed in type I esterase production, and between 2.2 and 3.1 times in type II esterase production.
Torres
Sebastián
S
Planta Piloto de Procesos Industriales Microbiológicos, Av. Belgrano y Pasaje Caseros, T4001 MVB Tucumán, Argentina.
Martínez
M Alejandra
MA
Pandey
Ashok
A
Castro
Guillermo R
GR
D001407:Bacillus / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D004789:Enzyme Activation; D004795:Enzyme Stability; D004950:Esterases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D009930:Organic Chemicals / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D013045:Species Specificity
D009930:Organic Chemicals; D012997:Solvents; D004950:Esterases