title: Influence of Nrf2 genotype on pulmonary NF-kappaB activity and inflammatory response after traumatic brain injury.
abstract: Inflammatory response plays an important role in the pathogenesis of acute lung injury (ALI) after traumatic brain injury (TBI). Nuclear factor erythroid 2-related factor 2 (Nrf2) is a key transcription factor that plays a crucial role in cytoprotection against inflammation. The present study explored the influence of Nrf2 genotype on the production of cytokines and on activation of transcription factors in a murine TBI model. Wild-type Nrf2 (+/+) and Nrf2 (-/-) deficient mice were subjected to a moderately severe weight-drop impact-acceleration head injury. Lung wet/dry weight ratio, tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1), and nuclear factor kapp-aB (NF-kappaB) were investigated at 24 hr after TBI. Nrf2 (-/-) mice were shown to have a greater increase in the lung wet/dry weight ratio compared to their wild-type Nrf2 (+/+) counterparts after TBI. This exacerbation of lung injury in Nrf2 (-/-) mice was associated with increased levels of TNF-alpha, IL-1beta, IL-6, ICAM-1, and their mediator, NF-kappaB. The results suggest that Nrf2 plays an important protective role in attenuating the pulmonary inflammatory response and NF-kappaB activation after TBI.
authors: Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
authors: Wei
authors: W
authors: Jin
authors: Lin
authors: L
authors: Zhu
authors: Qun
authors: Q
authors: Guan
authors: Gang
authors: G
authors: Chen
authors: Qing Feng
authors: QF
authors: Wang
authors: Hong Xia
authors: HX
authors: Yin
authors: Chun Hua
authors: CH
authors: Hang
authors: Ji Xin
authors: JX
authors: Shi
authors: Han Dong
authors: HD
authors: Wang
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000139:chemically induced / Q000150:complications* / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D005838:Genotype; D007249:Inflammation / Q000150:complications*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008813:Mice, Inbred ICR; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009929:Organ Size
chemical_list: D016207:Cytokines; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495636:Nfe2l2 protein, mouse; D018799:Intercellular Adhesion Molecule-1