title: Time-course of effects of external beam radiation on [18F]FDG uptake in healthy tissue and bone marrow.
abstract: The utility of PET for monitoring responses to radiation therapy have been complicated by metabolically active processes in surrounding normal tissues. We examined the time-course of [18F]FDG uptake in normal tissues using small animal-dedicated PET during the 2 month period following external beam radiation. Four mice received 12 Gy of external beam radiation, in a single fraction to the left half of the body. Small animal [18F]FDG-PET scans were acquired for each mouse at 0 (pre-radiation), 1, 2, 3, 4, 5, 8, 12, 19, 24, and 38 days following irradiation. [18F]FDG activity in various tissues was compared between irradiated and non-irradiated body halves before, and at each time point after irradiation. Radiation had a significant impact on [18F]FDG uptake in previously healthy tissues, and time-course of effects differed in different types of tissues. For example, liver tissue demonstrated increased uptake, particularly over days 3-12, with the mean left to right uptake ratio increasing 52% over mean baseline values (p < 0.0001). In contrast, femoral bone marrow uptake demonstrated decreased uptake, particularly over days 2-8, with the mean left to right uptake ratio decreasing 26% below mean baseline values (p = 0.0005). Significant effects were also seen in lung and brain tissue. Radiation had diverse effects on [18F]FDG uptake in previously healthy tissues. These kinds of data may help lay groundwork for a systematically acquired database of the time-course of effects of radiation on healthy tissues, useful for animal models of cancer therapy imminently, as well as interspecies extrapolations pertinent to clinical application eventually.
authors: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, USA.
authors: Adam L
authors: AL
authors: Kesner
authors: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, USA.
authors: Victoria K
authors: VK
authors: Lau
authors: Department of Radiation Oncology, David Geffen School of Medicine, University of California, Los Angeles, USA.
authors: Michael
authors: M
authors: Speiser
authors: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, USA.
authors: Wei-Ann
authors: WA
authors: Hsueh
authors: Department of Radiation Oncology, David Geffen School of Medicine, University of California, Los Angeles, USA.
authors: Nzhde
authors: N
authors: Agazaryan
authors: Department of Radiation Oncology, David Geffen School of Medicine, University of California, Los Angeles, USA.
authors: John J
authors: JJ
authors: DeMarco
authors: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, USA.
authors: Johannes
authors: J
authors: Czernin
authors: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, USA.
authors: Daniel Hs
authors: DH
authors: Silverman
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000000981:diagnostic imaging*; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D008099:Liver / Q000000981:diagnostic imaging; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D049268:Positron-Emission Tomography / Q000379:methods; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18