Gas chromatographic-mass spectrometric analysis of acrylamide and acetamide in cigarette mainstream smoke after on-column injection.
A method is described for the simultaneous determination of two short-chained amides, acrylamide and acetamide (classified by the International Agency for Research on Cancer as probable and possible human carcinogens, respectively), in total particulate matter using gas chromatography-on-column injection and mass spectrometric detection. Sample preparation is kept to a minimum, and the proposed analytical procedure proves to be fast, sensitive, and precise. Validation studies show good linearity with a regression coefficient of r2=.000 for both compounds. Quantitation limits are 32 ng/mL for acrylamide and 70 ng/mL for acetamide. In the particulate phase of mainstream smoke from the University of Kentucky Reference Cigarette 2R4F, 2.3 microg/cig acrylamide and 4.7 microg/cig acetamide are found; no acetamide and only .0074 microg/cig acrylamide is found in the gas phase. Possible mechanisms of formation in cigarette smoke are discussed.
Diekmann
Joerg
J
Philip Morris Research Laboratories GmbH, Fuggerstr. 3, D-51149 Köln, Germany. Joerg.Diekmann@pmintl.com
Wittig
Arno
A
Stabbert
Regina
R
D000081:Acetamides / Q000032:analysis*; D020106:Acrylamide / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D012906:Smoke / Q000032:analysis*; D014026:Nicotiana
D000081:Acetamides; D012906:Smoke; D020106:Acrylamide; C030686:acetamide