Molecular and genetic characterisation of the SARS coronavirus auxiliary protein X1 in Drosophila.
1. We have generated monoclonal antibodies against the SARS coronavirus (SARS-CoV) X1/3a protein (3a), which are suitable for western blotting, immunocytochemistry, and immunohistochemistry. 2. We have established and characterised an in-vivo 3a transgenic Drosophila model, and demonstrated its usefulness in studying SARS-CoV 3a gene function. 3. We validated our in-vivo findings on 3a gene function in mammalian Vero E6 cells. 4. Our findings raise the possibility of using ion channel blockers as a novel approach to suppress SARS-CoV-induced cell death.
Chan
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Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. hyechan@cuhk.edu.hk
Tsui
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Chan
C M
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Chan
C S
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Chen
Y W
YW
Law
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Wong
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A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology; D017209:Apoptosis / Q000235:genetics*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004330:Drosophila; D005164:Factor IX; D015967:Gene Expression Regulation, Viral; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D008967:Molecular Biology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012680:Sensitivity and Specificity; D014709:Vero Cells / Q000166:cytology / Q000502:physiology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D005164:Factor IX