Characterization of an extracellular polysaccharide produced by a Pseudomonas strain grown on glycerol.
A new extracellular charged polysaccharide composed mainly by galactose, with lower amounts of mannose, glucose and rhamnose, was produced by the cultivation of Pseudomonas oleovorans NRRL B-14682 using glycerol as the sole carbon source. Thermal and solid-state NMR analysis showed that this polymer is essentially amorphous, with a glass transition temperature of 155.7 degrees C. The exopolysaccharide aqueous solutions have viscoelastic properties similar to that of Guar gum, but with affinity to salts as a result of its polyelectrolyte character. In addition, the exopolysaccharide has demonstrated good flocculating and emulsifying properties and film-forming capacity. These properties make this polymer a good alternative to more expensive natural polysaccharides, such as Guar gum, in several applications in the food, pharmaceutical, cosmetic, textile, paper and petroleum industries.
Freitas
Filomena
F
CQFB/REQUIMTE, Chemistry Department, FCT/Universidade Nova de Lisboa, Caparica 2829-516, Portugal.
Alves
Vitor D
VD
Pais
Joana
J
Costa
Nuno
N
Oliveira
Cristina
C
Mafra
Luís
L
Hilliou
Loic
L
Oliveira
Rui
R
Reis
Maria A M
MA
D049109:Cell Proliferation; D004548:Elasticity; D005881:Gingiva / Q000737:chemistry; D005990:Glycerol / Q000378:metabolism*; D006244:Hardness; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D044204:Pseudomonas oleovorans / Q000737:chemistry* / Q000378:metabolism*; D013045:Species Specificity; D014783:Viscosity
D011135:Polysaccharides, Bacterial; D005990:Glycerol