[The expression of NGF after rat diffuse axonal injury].
OBJECTIVE
To study the expression of nerve growth factor (NGF) in diffuse axonal injury (DAI) in rat.


METHODS
Eighty SD rats were used and samples were taken at 1 h, 3 h, 6 h, 12 h, 24 h, 48 h, 3 d, and 7 d after brain injury. The expressions of NGF in cerebral cortex, thalamus, cerebellum, and hippocampus were studied with immunohistochemistry and compared with normal group and sham operation group.


RESULTS
Low expression of NGF was observed in normal group and sham operation group. The expression of NGF increased 1 h after injury, peaked at 12 h, and returned to basal level at day 7.


CONCLUSIONS
NGF is involved in repair of DAI. The changes of NGF expression following DAI may be applied to estimate the post-injury time interval of the brain in forensic medicine.
Chen
Zong-Yun
ZY
Department of Forensic Medicine, Wannan Medical College, Wuhu 241000, China. chenzongyun1979@yahoo.com.cn
Wang
Jing-Yu
JY
Li
Yong-Hong
YH
D000818:Animals; D001930:Brain Injuries / Q000378:metabolism*; D020833:Diffuse Axonal Injury / Q000378:metabolism*; D049429:Forensic Pathology; D008297:Male; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D009414:Nerve Growth Factors