[Determination of streptomycin residue in royal jelly by high performance liquid chromatography with post-column derivatization].
A reliable and sensitive method was introduced for the analysis of streptomycin in royal jelly. The method was based on a separation by a C8 analytical column with beta-naphthoquinone-4-sulfonate post-column derivatization and fluorescence detection. The clean-up of the extract was done by solid-phase extraction, firstly with an octadecyl cartridge and secondly with a cation-exchange cartridge. The linear range was 0.02 - 0.5 mg/L and the correlation coefficient was 0.995 8. The limits of detection (S/N = 3) and quantitation (S/N = 10) of streptomycin were 0.005 mg/kg and 0.01 mg/kg, respectively. The recoveries ranged from 84.0% to 104.0% with the relative standard deviations not larger than 7.9%. The method reduced the possibility of the false positive, and it could meet the need of the current work.
Zhang
Xiaoyan
X
Animal, Plant and Food Inspection Center (APFIC) of Jiangsu Entry-Exit Inspection and Quarantine Bureau, National Honey Reference Laboratory, Nanjing 210001, China. zhangxy@jsciq.gov.cn
Xu
Jinzhong
J
Shen
Chongyu
C
Chen
Huilan
H
Wu
Bin
B
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005227:Fatty Acids / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013307:Streptomycin / Q000032:analysis*; D000099219:Royal Jelly
D005227:Fatty Acids; D012997:Solvents; D013307:Streptomycin; D000099219:Royal Jelly