title: Construction of a high-performance human fetal liver-derived lentiviral cDNA library.
abstract: The gene transduction method is a very powerful tool, not only in basic science but also in clinical medicine. Regenerative medicine is one field that has close connection with both basic and clinical. Recently, it has been reported that induced pluripotent stem (iPS) cells can be produced from somatic cells by a three or four gene transduction. We have also recently reported that lentiviral gene transfer of the tal1/scl gene can efficiently differentiate non-human primate common marmoset ES cells into hematopoietic cells without the support of stromal cells. In this study, we constructed a high-performance human fetal liver-derived lentiviral expression library, which contains a high number of individual clones, in order to develop a very helpful tool for understanding early hematopoiesis and/or hepatocytosis for future regenerative medicine. Our lentiviral cDNA library consisted of more than 8 x 10(7) individual clones, and their average insert size was >2 kb. DNA sequence analysis for each individual inserted cDNAs revealed that >60% contained the full-length protein-coding regions for many genes including cytokine receptors, cytoplasmic proteins, protein inhibitors, and nuclear factors. The transduction efficiency on 293T cells was 100% and the average size of an integrated cDNA was ~1.1 kb. These results suggest that our lentiviral human fetal liver cDNA expression library could be a very helpful tool for accelerating the discovery of novel genes that are involved in early hematopoiesis and hepatopoiesis and to make the use of iPS cells more efficient in the field of regenerative medicine.
authors: Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, Maidashi, Higashi-ku, Fukuoka, Japan.
authors: Ryo
authors: R
authors: Kurita
authors: Tatsuo
authors: T
authors: Oikawa
authors: Michiyo
authors: M
authors: Okada
authors: Tomoko
authors: T
authors: Yokoo
authors: Yuusuke
authors: Y
authors: Kurihara
authors: Yuko
authors: Y
authors: Honda
authors: Rui
authors: R
authors: Kageyama
authors: Youko
authors: Y
authors: Suehiro
authors: Toshihiko
authors: T
authors: Okazaki
authors: Mutsunori
authors: M
authors: Iga
authors: Hiroyuki
authors: H
authors: Miyoshi
authors: Kenzaburo
authors: K
authors: Tani
mesh_terms: D000818:Animals; D002144:Callithrix; D002460:Cell Line; D005333:Fetus; D015723:Gene Library; D006410:Hematopoiesis / Q000235:genetics*; D006801:Humans; D016086:Lentivirus; D014161:Transduction, Genetic