Antiurolithic effect of lupeol and lupeol linoleate in experimental hyperoxaluria.
The present study was undertaken to explore the efficiency of the pentacyclic triterpene lupeol (1) and its ester derivative, lupeol linoleate (2), in experimental hyperoxaluria. Hyperoxaluria was induced in male Wistar rats with 0.75% ethylene glycol (EG) in drinking water for 28 days. Hyperoxaluric animals were supplemented orally with 1 and 2 (50 mg/kg body wt/day) throughout the experimental period of 28 days. The renal enzymes were assayed as markers of renal tissue integrity. The redox status and oxalate metabolism in animals under oxalate overloading was also assessed. Microscopic analysis was done to investigate the abnormalities associated with oxalate exposure in renal tissues. Increase in oxidative milieu in hyperoxaluria was evident by increased lipid peroxidation (LPO) and decreased enzymic and nonenzymic antioxidants. Decrease in the activities of renal enzymes exemplified the damage induced by oxalate, which correlated positively with increased LPO and increased oxalate synthesis. Renal microscopic analysis further emphasized the oxalate-induced damage. These abnormal biochemical and histological aberrations were attenuated with test compound treatment, with 2 more effective than 1. From the present study, it can be concluded that 1 and 2 may serve as candidates for alleviating oxalate toxicity.
Sudhahar
Varatharajan
V
Department of Medical Biochemistry, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, India.
Veena
Coothan Kandaswamy
CK
Varalakshmi
Palaninathan
P
D000284:Administration, Oral; D000818:Animals; D004195:Disease Models, Animal; D019855:Ethylene Glycol / Q000494:pharmacology; D006959:Hyperoxaluria / Q000139:chemically induced* / Q000188:drug therapy*; D007669:Kidney Calculi / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010070:Oxalates / Q000633:toxicity*; D053978:Pentacyclic Triterpenes; D051381:Rats; D017208:Rats, Wistar; D014315:Triterpenes / Q000737:chemistry* / Q000627:therapeutic use*; D052878:Urolithiasis / Q000517:prevention & control*; D008041:Linoleic Acids; D000094042:Lupanes
D019855:Ethylene Glycol; D010070:Oxalates; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C403497:lupeol linoleate; D008041:Linoleic Acids; D000094042:Lupanes; C010480:lupeol