Osteogenic-related gene expression profiles of human dental follicle cells induced by dexamethasone.
OBJECTIVE
Human dental follicle cells (hDFC) have the ability to differentiate into mineralized tissue-forming cells during root and periodontal development or osteogenic induction in vitro. The present study aimed to validate the osteogenic induction of hDFC by dexamethasone (DEX) and to explore the changes of related genes responsible for the osteogenic differentiation process.


METHODS
Passage-cultured hDFC were induced by DEX and analyzed for mineralization activity by morphological observation, alkaline phosphatase (ALP) activity, and alizarin red S staining. GEArray Q series human osteogenesis gene array was used to describe large-scale gene expression in treated hDFC compared to the control group. Quantitative real-time RT-PCR was performed to confirm the microarray data by analyzing the expression of 7 critical transcripts.


RESULTS
Osteogenic differentiation of hDFC was confirmed by morphological change, elevated ALP activity and calcified nodules. In 96 genes investigated through the microarray analysis, 20 genes were upregulated and 8 genes were downregulated more than 2-fold. The results of the real-time RT-PCR correlated with the microarray analysis. The expression of the transforming growth factor-beta superfamily showed varying degrees of increase, and fibroblast growth factors exhibited a differential changing trend of expression. The expression of most types of collagen genes representative of extracellular matrixes increased under DEX treatment while small mothers against decapentaplegic 6 and 7 expressions significantly decreased.


CONCLUSIONS
Our results demonstrated that hDFC displayed osteoblastic features in both phenotypic and genotypic traits induced by DEX in vitro.
Jin
Zuo-lin
ZL
Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China. zuolinj@fmmu.edu.cn
Zhang
Yong-kuan
YK
Sun
Hai-yan
HY
Lin
Zhu
Z
Bi
Ying-chun
YC
Duan
Yin-zhong
YZ
Ding
Yin
Y
D000293:Adolescent; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001846:Bone Development / Q000187:drug effects* / Q000235:genetics*; D002454:Cell Differentiation / Q000187:drug effects; D002648:Child; D003795:Dental Sac / Q000187:drug effects* / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D066298:In Vitro Techniques; D008964:Molar, Third / Q000166:cytology / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000893:Anti-Inflammatory Agents; D003907:Dexamethasone