Exploring the nature of photo-damage in two-photon excitation by fluorescence intensity modulation.
We investigate the relative photo-damage effects during one- and two-photon excitations and demonstrate that there exist fundamental differences in the damage induced by a high repetition rate laser as compared to that of a CW laser. This difference is evident from the degree of enhanced fluorescence intensity achieved by blanking the excitation with an optical chopper. Such an enhancement in fluorescence intensity provides better signal-to-noise ratio that could have immediate applications in multiphoton imaging of live specimens.
De
Arijit Kumar
AK
Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, UP-208016, India.
Goswami
Debabrata
D
D005453:Fluorescence; D007834:Lasers / Q000009:adverse effects; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods / Q000592:standards