In vivo labeling of adult neural progenitors for MRI with micron sized particles of iron oxide: quantification of labeled cell phenotype.
The subventricular zone (SVZ) is a continual source of neural progenitors throughout adulthood. Many of the animal models designed to study the migration of these cells from the ventricle to places of interest like the olfactory bulb or an injury site require histology to localize precursor cells. Here, it is demonstrated that up to 30% of the neural progenitors that migrate along the rostral migratory stream (RMS) in an adult rodent can be labeled for MRI via intraventricular injection of micron sized particles of iron oxide (MPIOs). The precursors migrating from the SVZ along the RMS were found to populate the olfactory bulb with all three types of neural cells; neurons, oligodendrocytes, and astrocytes. In all cases 10-30% of these cells were labeled in the RMS en route to the olfactory bulb. Ara-C, an anti-mitotic agent, eliminated precursor cells at the SVZ, RMS, and olfactory bulb and also eliminated the MRI detection of the precursors. This indicates that the MRI signal detected is due to progenitor cells that leave the SVZ and is not due to non-specific diffusion of MPIOs. Using MRI to visualize neural progenitor cell behavior in individual animals during plasticity or disease models should be a useful tool, especially in combination with other information that MRI can supply.
Sumner
James P
JP
Laboratory of Functional and Molecular Imaging, NINDS, NIH, Bethesda, MD 20892, USA.
Shapiro
Erik M
EM
Maric
Dragan
D
Conroy
Richard
R
Koretsky
Alan P
AP
D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D002214:Capsules; D002452:Cell Count / Q000379:methods*; D002478:Cells, Cultured; D003287:Contrast Media; D005290:Ferric Compounds / Q000493:pharmacokinetics; D007089:Image Enhancement / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
D002214:Capsules; D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide