Thermosensitive polymer-conjugated albumin nanospheres as thermal targeting anti-cancer drug carrier.
Thermosensitive Poly(N-isopropylacrylamide-co-acrylamide-co-allylamine) (PNIPAM-AAm-AA)-conjugated albumin nanospheres (PAN) was developed as a new thermal targeting anti-cancer drug carrier by conjugating PNIPAM-AAm-AA on the surface of albumin nanospheres (AN). AN with diameter below 200nm and narrow size distribution was successfully prepared in the first step with desolvation technique. PNIPAM-AAm-AA with different molecular weight (M(w)) was synthesized in the second step by radical polymerization and conjugated onto the surface of AN. Anti-cancer drug adriamycin (ADR) was then entrapped into the AN and PAN during the particle preparation. Compared with AN, the release rate of ADR from PAN in trypsin solution was slower, and decreased with increasing the conjugation amounts (hairy density) or M(w) of PNIPAM-AAm-AA (hairy length). Moreover, the release of ADR from PAN above the cloud-point temperature (T(cp)) of PNIPAM-AAm-AA became faster due to shrinkage of hairy thermosensitive polymer. To testify the thermal targetability in vivo, PAN was incubated with HepG2 cells. As expected, PAN can target cancer cells above the T(cp) of PNIPAM-AAm-AA, whereas it cannot below the T(cp). These results might reflect that PAN may selectively accumulate onto solid tumors that are maintained above physiological temperature due to local hyperthermia.
Shen
Zheyu
Z
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
Wei
Wei
W
Zhao
Yongjiang
Y
Ma
Guanghui
G
Dobashi
Toshiaki
T
Maki
Yasuyuki
Y
Su
Zhiguo
Z
Wan
Jinpei
J
D000180:Acrylic Resins / Q000737:chemistry*; D000418:Albumins / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry*; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D004553:Electric Conductivity; D005453:Fluorescence; D006801:Humans; D008027:Light; D046529:Microscopy, Electron, Transmission; D054118:Nanospheres; D010316:Particle Size; D011108:Polymers; D012542:Scattering, Radiation; D012995:Solubility; D013696:Temperature; D013816:Thermodynamics
D000180:Acrylic Resins; D000418:Albumins; D000970:Antineoplastic Agents; D004337:Drug Carriers; D011108:Polymers; C534710:poly(N-isopropylacrylamide-co-acrylamide-co-allylamine); C052970:poly-N-isopropylacrylamide; D004317:Doxorubicin