Blocking E-selectin inhibits ischaemia-reperfusion-induced neutrophil recruitment to the murine testis.
Germ cell-specific apoptosis that occurs after ischaemia-reperfusion (IR) of the murine testis is dependent on neutrophil recruitment to the testis and is dependent upon the cell adhesion molecule E-selectin. In this study, we aimed to inhibit neutrophil recruitment to the IR-induced testis. Mice were subjected to a 2-h period of testicular torsion (ischaemia) followed by detorsion (reperfusion). Shortly after onset of reperfusion, mice received either a function-blocking monoclonal anti-mouse E-selectin antibody (FBmAb) or isotype-matched control antibody. Mice were killed 24 h after reperfusion and cells isolated from the testis were analysed for the presence of neutrophil infiltration by flow cytometry. Administration of FBmAb inhibited neutrophil recruitment to the IR-induced testis dramatically. Therefore, blockage of E-selectin may be a strategy to treat post-ischaemic testis.
Celebi
M
M
Cardiovascular Division, University of Virginia School of Medicine, Charlottesville, VA, USA. muzaffercelebi2002@yahoo.com
Paul
A G A
AG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D019040:E-Selectin / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000473:pathology*; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology* / Q000628:therapy; D013737:Testis / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D019040:E-Selectin