title: An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads.
abstract: This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA or DNA.
authors: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.
authors: Crystale Siew Ying
authors: CS
authors: Lim
authors: Chee Hong
authors: CH
authors: Tung
authors: Rozita
authors: R
authors: Rosli
authors: Pei Pei
authors: PP
authors: Chong
mesh_terms: D002175:Candida / Q000737:chemistry* / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D005821:Genetic Techniques; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D013012:Sorbitol / Q000737:chemistry
chemical_list: D004271:DNA, Fungal; D012331:RNA, Fungal; D013012:Sorbitol