title: Influence of temperature and soil water content on bacterial, archaeal and denitrifying microbial communities in drained fen grassland soil microcosms.
abstract: In this study, microcosms were used to investigate the influence of temperature (4 and 28 degrees C) and water content (45% and 90% WHC) on microbial communities and activities in carbon-rich fen soil. Bacterial, archaeal and denitrifier community composition was assessed during incubation of microcosms for 12 weeks using terminal restriction fragment length polymorphism (T-RFLP) profiling of 16S rRNA and nitrous oxide reductase (nosZ) genes. In addition, microbial and denitrifier abundance, potential denitrification activity and production of greenhouse gases were measured. No detectable changes were observed in prokaryote or denitrifier abundance. In general, cumulatively after 12 weeks more carbon was respired at the higher temperature (3.7 mg CO(2) g(-1) soil), irrespective of the water content, whereas nitrous oxide production was greater under wet conditions (98-336 microg N(2)O g(-1) soil). After an initial lag phase, methane emissions (963 microg CH(4) g(-1) soil) were observed only under warm and wet conditions. T-RFLP analyses of bacterial 16S rRNA and nosZ genes revealed small or undetectable community changes in response to temperature and water content, suggesting that bacterial and denitrifying microbial communities are stable and do not respond significantly to seasonal changes in soil conditions. In contrast, archaeal microbial community structure was more dynamic and was strongly influenced by temperature.
authors: Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Vecna pot, Ljubljana, Slovenia.
authors: Blaz
authors: B
authors: Stres
authors: Tjasa
authors: T
authors: Danevcic
authors: Levin
authors: L
authors: Pal
authors: Mirna Mrkonjić
authors: MM
authors: Fuka
authors: Lara
authors: L
authors: Resman
authors: Simona
authors: S
authors: Leskovec
authors: Janez
authors: J
authors: Hacin
authors: David
authors: D
authors: Stopar
authors: Ivan
authors: I
authors: Mahne
authors: Ines
authors: I
authors: Mandic-Mulec
mesh_terms: D001105:Archaea / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D015169:Colony Count, Microbial; D019641:DNA, Archaeal / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D019766:Genes, Archaeal; D005798:Genes, Bacterial; D020459:Genes, rRNA; D008697:Methane / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D006109:Poaceae / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D013696:Temperature; D014867:Water
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil; D014867:Water; D002245:Carbon Dioxide; D010088:Oxidoreductases; C022763:nitrous oxide reductase; D009609:Nitrous Oxide; D008697:Methane