Genetic manipulation of Campylobacter jejuni.
Molecular manipulation has been a limiting factor in C. jejuni research for many years. Recent advances in molecular techniques adapted for C. jejuni have furthered our understanding of the organism. This unit is dedicated to common molecular tools in bacterial research specifically tailored for C. jejuni. These include colony PCR, DNA isolation, and RNA isolation. The unit also reviews techniques for genetic manipulation, such as the use of plasmids, natural transformation, electroporation, conjugation, and transposition. In addition, a reporter system, the arylsulfatase assay, can be used to study gene expression.
Davis
Lindsay
L
Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.
Young
Kathryn
K
DiRita
Victor
V
D001192:Arylsulfatases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D016123:Campylobacter jejuni / Q000201:enzymology / Q000235:genetics*; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D001192:Arylsulfatases