[Effect of decitabine combined with Trichostatin A on MDS cell line SKM-1 in vitro].
The study was purposed to explore the effect and mechanisms of decitabine and/or Trichostatin A (TSA) on SKM-1 cells in vitro. The effect of decitabine and/or TSA on proliferation of SKM-1cells was analyzed with trypan blue exclusion; the differentiation of SKM-1 cells was detected by nitro-blue tetrazolium (NBT) reduction and flow cytometry; the apoptosis of cells was measured by Annexin V-FITC; the mRNA expression of Fas, survivin and P15(INK4B) in cells treated with decitabine and/or TSA was evaluated by RT-PCR. The results showed that decitabine and/or TSA were capable of inhibiting SKM-1 cell growth and promoting cell differentiation; they stimulated the expression of CD14 and CD11b and inhibited HLA-DR expression; meanwhile and decitabine or/and TSA could induce cell apoptosis, up-regulate mRNA expression of Fas and P15(INK4B), and down-regulate survivin mRNA expression. It is concluded that decitabine can induce apoptosis/differentiation of SKM-1 cells, whose mechanisms may related to the expression of Fas, survivin and P15(INK4B). Decitabine has the synergistic effect with TSA.
Yang
Li
L
Department of Hematology, The Affiliated Hospital, Nantong University, Nantong 226001, Jiangsu Province, China.
Xu
Rui-Rong
RR
Song
Guo-Qi
GQ
Hang
Hong-Ming
HM
Liu
Hong
H
Jiang
Sheng-Hua
SH
Wang
Xin-Feng
XF
Ding
Xun-Sheng
XS
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D000077209:Decitabine; D004357:Drug Synergism; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D000077022:Survivin; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism
D000964:Antimetabolites, Antineoplastic; C107489:BIRC5 protein, human; D006877:Hydroxamic Acids; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D000077022:Survivin; D019014:fas Receptor; C012589:trichostatin A; D000077209:Decitabine; D001374:Azacitidine