[Inactivation of AMP-deaminase from white muscle of Cyprinus carpio in the systems with free radical oxidation].
The purification and in vitro inactivation of AMP-deaminase from white muscle of carp Cyprinus carpio were conducted in the Fe2+/H2O2 and Fe2+/ascorbate oxidation systems. The enzyme activity decreases by 50% within 30 minutes of incubation in the presence of 100 microM of hydrogen peroxide and 5 microM of ferrous sulfate. Inactivation depended on incubation time and concentrations of FeSO4 and H2O2. In the system Fe2+/ascorbate the enzyme activity decreased by 50% at concentration of ascorbate 1 mM and 5 ferrous sulfate microM. Sodium nitrite did not affect the activity. S(0.5) and n(H) of both native and partially inactivated enzymes were virtually the same, while maximal activity of the inactivated enzyme was 2-3-fold lower than that of the native one.
Husak
V V
VV
Lushchak
V I
VI
D000659:AMP Deaminase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry / Q000494:pharmacology; D002347:Carps / Q000378:metabolism*; D005296:Ferrous Compounds / Q000737:chemistry / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000201:enzymology*; D016877:Oxidants / Q000737:chemistry / Q000494:pharmacology; D010084:Oxidation-Reduction; D013997:Time Factors
D005296:Ferrous Compounds; D016877:Oxidants; C020748:ferrous sulfate; D006861:Hydrogen Peroxide; D000659:AMP Deaminase; D001205:Ascorbic Acid