title: Late steps of parvoviral infection induce changes in cell morphology.
abstract: Previously, virus-induced non-filopodial extensions have not been encountered in connection with viral infections. Here, we report emergence of long extensions protruding from Norden laboratory feline kidney (NLFK) and A72 (canine fibroma) cells infected with canine parvovirus for 72 h. These extensions significantly differ in length and number from those appearing in control cells. The most striking feature in the extensions is the length, reaching up to 130 microm, almost twice the average length of a healthy NLFK cell. In A72 cells, the extensions were even longer, up to 200 microm. The results presented here also suggest that the events leading to the growth of these extensions start earlier in infection and abnormal extension growth is detectable already at 24-h post-infection (p.i.). These extensions may have a vital role in the cell-to-cell transmission of the virus.
authors: Department of Biological and Environmental Science and Nanoscience Center, P.O. Box 35, University of Jyväskylä, FIN-40014 Jyväskylä, Finland. kiinpakk@bytl.jyu.fi
authors: Kirsi
authors: K
authors: Pakkanen
authors: Jonna
authors: J
authors: Nykky
authors: Matti
authors: M
authors: Vuento
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D048430:Cell Shape; D022081:Cell Surface Extensions / Q000737:chemistry / Q000821:virology*; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000502:physiology*