What's happening--probing of HPA-1a antigen-antibody interaction by surface plasmon resonance technology.
This brief report summarizes the use of surface plasmon resonance technology (SPRT) in probing HPA-1a antigen-antibody interactions, based on a poster presented at the 60th meeting of the American Association of Blood Banks. It was concluded that the GP purification method could affect the performance of antigen in SPRT. It also highlighted that chips immobilised with Monoclonal antibody (Mab)-purified GP-IIb/IIIa work satisfactorily with both monoclonal and recombinant Abs with the appropriate concentration and binding affinity, while determination of the avidity and concentration of maternal polyclonal antibodies in respect to clinical severity on NAIT warrants further development.
Bessos
H
H
Immunohaematology R&D Theme Group, Scottish National Blood Transfusion Service, Edinburgh, UK.
Matviyenko
M
M
Brown
P
P
Husebekk
A
A
Killie
M K
MK
Seghatchian
J
J
Santoso
S
S
Urbaniak
S J
SJ
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D000915:Antibody Affinity; D016824:Antigens, Human Platelet / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003199:Computer Systems; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D039661:Integrin beta3 / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D007518:Isoantibodies / Q000276:immunology*; D008297:Male; D011247:Pregnancy; D040081:Protein Array Analysis; D011485:Protein Binding; D020349:Surface Plasmon Resonance / Q000295:instrumentation
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D007518:Isoantibodies; C083451:human platelet antigen 1b