title: Wuweizisu C from Schisandra chinensis decreases membrane potential in C6 glioma cells.
abstract: OBJECTIVE
  To study the effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis, such as wuweizisu C, gomisin N, gomisin A, and schisandrin, on the membrane potential in C6 glioma cells.
  
  
  METHODS
  The membrane potential was estimated by measuring the fluorescence change in DiBAC-loaded glioma cells.
  
  
  RESULTS
  Wuweizisu C decreased the membrane potential in a concentration-dependent manner. Gomisin N and gomisin A, however, showed differential modulation and no change was induced by schisandrin or dimethyl- 4,4'-dimethoxy-5,6,5',6'-dimethylene dioxybiphenyl-2,2'-dicarboxylate, a synthetic drug derived from dibenzocyclooctadiene lignans. We found no involvement of G(i/o ) proteins, phospholipase C, and extracellular Na(+) on the wuweizisu C-induced decrease of the membrane potential. Wuweizisu C by itself did not change the intracellular Ca(2+)[Ca(2+)](i) concentration, but decreased the ATP-induced Ca(2+) increase in C6 glioma cells. The 4 lignans at all concentrations used in this study did not induce any effect on cell viability. Furthermore, we found a similar decrease of the membrane potential by wuweizisu C in PC12 neuronal cells.
  
  
  CONCLUSIONS
  Our results suggest that the decrease in the membrane potential and the modulation of [Ca(2+)](i) concentration by wuweizisu C could be important action mechanisms of wuweizisu C.
authors: Department of Horticultural Bioscience, Pusan National University, Miryang-si 627-706, Republic of Korea.
authors: Young-whan
authors: YW
authors: Choi
authors: Kyeok
authors: K
authors: Kim
authors: Ji-yeong
authors: JY
authors: Jo
authors: Hyo-lim
authors: HL
authors: Kim
authors: You-jin
authors: YJ
authors: Lee
authors: Woo-jung
authors: WJ
authors: Shin
authors: Santosh-J
authors: SJ
authors: Sacket
authors: Mijin
authors: M
authors: Han
authors: Dong-soon
authors: DS
authors: Im
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D034242:Cyclooctanes / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000378:metabolism; D005910:Glioma / Q000503:physiopathology; D006801:Humans; D007202:Indicators and Reagents; D017705:Lignans / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects*; D016716:PC12 Cells; D011083:Polycyclic Compounds / Q000494:pharmacology*; D051381:Rats; D032141:Schisandra / Q000737:chemistry*; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D034242:Cyclooctanes; D007202:Indicators and Reagents; D017705:Lignans; D011083:Polycyclic Compounds; C031409:schizandrin C; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins; D002118:Calcium