title: Establishment and functional characterization of a tracheal epithelial cell line RTEC11 from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen.
abstract: A tracheal epithelial cell line RTEC11 was established from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen. The cells grew continuously at a permissive temperature of 33 degrees C but not at a non-permissive temperature of 39 degrees C. Morphological and functional investigations demonstrated that the cells were polarized epithelial cells maintaining a regulated permeability barrier function. Interestingly, the expression levels of Muc1 (mucin 1) and Scgb1a1 (uteroglobin), non-ciliated secretory cell markers, and Tubb4 (tubulin beta 4), a ciliated cell marker, were significantly increased under the cell growth-restricted condition. Global gene expression and computational network analyses demonstrated a significant genetic network associated with cellular development and differentiation in cells cultured at the non-permissive temperature. The tracheal epithelial cell line RTEC11 with unique characteristics should be useful as an in vitro model for studies of the physiological functions and gene expression of tracheal epithelial cells.
authors: Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Toyama, Japan. ytabu@cts.u-toyama.ac.jp
authors: Yoshiaki
authors: Y
authors: Tabuchi
authors: Takeshi
authors: T
authors: Doi
authors: Ichiro
authors: I
authors: Takasaki
authors: Ri-ichi
authors: R
authors: Takahashi
authors: Masatsugu
authors: M
authors: Ueda
authors: Yoshihisa
authors: Y
authors: Suzuki
authors: Masuo
authors: M
authors: Obinata
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics* / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000235:genetics; D049109:Cell Proliferation; D002923:Cilia / Q000378:metabolism; D003198:Computer Simulation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D008954:Models, Biological; D018396:Mucin-1 / Q000378:metabolism; D016571:Neural Networks, Computer; D051381:Rats; D055647:Rats, Transgenic; D013696:Temperature; D014132:Trachea / Q000166:cytology / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism; D014598:Uteroglobin
chemical_list: D000952:Antigens, Polyomavirus Transforming; D015415:Biomarkers; D002352:Carrier Proteins; C506177:MUC1 protein, rat; D018396:Mucin-1; C556391:Scgb1a1 protein, rat; D014404:Tubulin; D014598:Uteroglobin