title: Mechanism of enhancement of prochymosin renaturation by solubilization of inclusion bodies at alkaline pH.
abstract: The renaturation efficiency of recombinant prochymosin depends on not only the renaturation conditions but also the solubilization (denaturation) conditions. Compared with pH 8, solubilization of prochymosin-containing inclusion bodies at pH 11 (8 mol/L urea) results in onefold increase of renaturation efficiency ( approximately 40% vs. approximately 20 %). Alkaline pH facilitates the solubilization of inclusion bodies via the breakage of intermolecular disulfide bonds. Moreover, alkaline pH renders prochymosin molecules to be in a more reduced and more unfolded state which undergoes refolding readily.
authors: Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, China.
authors: Z
authors: Z
authors: Zhang
authors: Y
authors: Y
authors: Zhang
authors: K
authors: K
authors: Yang