Enhancement of gene expression efficiency using cationic liposomes on ovarian cancer cells.
We performed transfection using cationic liposome. According to the gene expression level, lined cells were divided into two groups, high and low. Introduced gene was monitored with a confocal laser-scanning microscope. The percentages of the cells introduced gene reached more than 90% in all line. Then, introduced gene was stable in high group, while in low group, it significantly decreased. With lysosomotrophic agents, gene expression efficiency was significantly reduced.With colchicine, gene expression efficiency did not change in high group, but was significantly elevated in low group. A method of liposomal transfection could be effective, particularly in low group.
Serikawa
Takehiro
T
Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Niigata, Japan. takehiro-s@med.niigata-u.ac.jp
Kikuchi
Hiroshi
H
Oite
Takashi
T
Tanaka
Kenichi
K
D000643:Ammonium Chloride / Q000494:pharmacology; D002412:Cations; D045744:Cell Line, Tumor; D002738:Chloroquine / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008081:Liposomes; D018613:Microscopy, Confocal; D010051:Ovarian Neoplasms; D014162:Transfection / Q000379:methods*
D002412:Cations; D008081:Liposomes; D000643:Ammonium Chloride; D049452:Green Fluorescent Proteins; D002738:Chloroquine; D003078:Colchicine