title: Development of monoclonal antibodies specific for the human sodium-dependent phosphate co-transporter NaPi2b.
abstract: Homeostasis of inorganic phosphate in the human body is maintained by regulated absorption, metabolism, and excretion. Sodium-dependent phosphate transporters (NaPi) mediate the transport of inorganic phosphate (P(i)) in cells in response to dietary phosphate consumption, hormones, and growth factors. NaPi2b is a member of the sodium-dependent phosphate transporter family, with a distinct pattern of expression and regulation. Signaling pathways activated by mitogens, glucocorticoids, and metabolic factors have been implicated in regulating P(i) transport via NaPi2b. Inactivation of NaPi2b function by mutations has been linked to human pathologies, such as pulmonary alveolar microlithiasis. In this study, we describe the generation and characterization of monoclonal antibodies against human NaPi2b. The monoclonal antibodies were shown to recognize specifically transiently overexpressed and endogenous NaPi2b in commonly used immunoassays, including Western blotting, immunoprecipitation, and immunohistochemistry. These properties make them particularly valuable reagents for elucidating NaPi2b function in health and disease.
authors: Department of Cell Signaling, Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv, Ukraine.
authors: Ramziya
authors: R
authors: Kiyamova
authors: Vitalina
authors: V
authors: Gryshkova
authors: Galina
authors: G
authors: Ovcharenko
authors: Dmytro
authors: D
authors: Lituyev
authors: Sergyy
authors: S
authors: Malyuchik
authors: Vasiliy
authors: V
authors: Usenko
authors: Yuliya
authors: Y
authors: Khozhayenko
authors: Vadym
authors: V
authors: Gurtovyy
authors: Beatrice
authors: B
authors: Yin
authors: Gerd
authors: G
authors: Ritter
authors: Lloyd
authors: L
authors: Old
authors: Valeriy
authors: V
authors: Filonenko
authors: Ivan
authors: I
authors: Gout
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D002277:Carcinoma / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007118:Immunoassay / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D010051:Ovarian Neoplasms / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D012680:Sensitivity and Specificity; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; C493557:SLC34A2 protein, human; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb