Upregulation of matrix metalloproteinase-9 dependent on hyaluronan synthesis after sciatic nerve injury.
Using a rat chronic constriction injury (CCI) model, we analyzed the expression and activity of matrix metalloproteinase-9 (MMP-9) after sciatic nerve injury and its relationship with hyaluronan (HA). MMP-9 expression and activity were induced after the nerve injury. We found that CCI rats with oral administration of 4-methylunbelliferone (4-MU), which was a hyaluronan synthases inhibitor, exhibited reduced MMP-9 activity and mRNA expression compared with CCI rats that were not given 4-MU. MMP modulatory factors, such as TIMPs and uPA, did not change after the 4-MU was administered. This result indicated that the upregulation of MMP-9 after nerve injury in CCI rats was dependent on HA synthesis and was useful for the treatment of nerve injuries.
Yong
Nie
N
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China. nieyong@gmail.com
Guoping
Cai
C
D000818:Animals; D003251:Constriction, Pathologic; D006820:Hyaluronic Acid / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D006923:Hymecromone / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries*; D015854:Up-Regulation
D006923:Hymecromone; D006820:Hyaluronic Acid; D020780:Matrix Metalloproteinase 9