Development of a sialic acid-containing hydrogel of poly[N-(2-hydroxypropyl) methacrylamide]: characterization and implantation study.
This study describes the preparation and the characterization of poly[ N-(2-hydroxypropyl methacrylamide)] hydrogel with bulk-modified saccharidic portion of ganglioside GM 3 (Neu5Ac-alpha2,3-Gal-beta1,4-Glc). The 3'-sialyllactose is a bioactive epitope recognized by many cell surface receptors on viruses, bacteria, and human cells such as growth factor receptors. Acrylated 3'-sialyllactose was synthesized and incorporated into the macromolecular network of hydrogels by free radical cross-linking copolymerization. Fluorescence techniques coupled to confocal laser scanning microscopy was employed to characterize the binding and accessibility of the sialyl group in the polymer network by using a monoclonal antibody against GM 3 and the lectin wheat germ agglutinin. The morphology of the network was examined by scanning electron microscopy and confocal microscopy to image the gel morphology. The water content of sialyllactosyl-HPMA hydrogel compared to unmodified gel was characterized by swelling measurements and thermogravimetry. A preliminary implantation study in rat brain was performed to examine the biofunctionality of the sialyllactosyl hydrogel using an experimental model of Parkinson's disease.
Woerly
Stéphane
S
Centre de Recherches sur les Macromolecules Vegetales (CERMAV-CNRS), BP 53, 38041 Grenoble Cedex 09, France. stephane.woerly@cermav.cnrs.fr
Fort
Sébastien
S
Pignot-Paintrand
Isabelle
I
Cottet
Cécile
C
Carcenac
Carole
C
Savasta
Marc
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001369:Axons / Q000473:pathology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D003342:Corpus Striatum / Q000473:pathology; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D005453:Fluorescence; D005679:G(M3) Ganglioside / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D007150:Immunohistochemistry; D020007:Implants, Experimental; D008422:Materials Testing; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D016627:Oxidopamine; D010316:Particle Size; D037121:Plant Lectins / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014909:Wheat Germ Agglutinins / Q000737:chemistry
C421467:3'-sialyllactose; D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D005679:G(M3) Ganglioside; D020100:Hydrogels; D009844:Oligosaccharides; D037121:Plant Lectins; D011109:Polymethacrylic Acids; D014909:Wheat Germ Agglutinins; C011533:Duxon; D016627:Oxidopamine; D004298:Dopamine