title: [Separation and purification of flavonoids from Smilax glabra by macroporous adsorption resin].
abstract: OBJECTIVE
  To investigate the process of separating and purifying flavonoids from Smilax glabra.
  
  
  METHODS
  With the yield of flavonoids as index, the optimum process of separating and purifying flavonoids from S. glabra Roxb was screened by static and dynamic adsorption tests.
  
  
  RESULTS
  The static saturated adsorption capacity of D101 macroporous resin to flavonoids of S. glabra was 45.6 mg x g(-1) (dry resin). The optimum conditions of dynamic adsorption and elution were as that the pH, the concentration, the adsorption velocity of the extracting solution, and the adsorption capacity were 6.00 +/- 0.20, 4.2 mg x mL(-1), 2 mL x min(-1) and 15 mL, respectively. The adsorbed resin column was washed by 100 mL 60% ethanol with pH value of 8.00 +/- 0.20 at the eluting velocity of 3 mL x min(-1) after washed by 100 mL distilled water.
  
  
  CONCLUSIONS
  The flavonoids of S. glabra was able to be easily separated and purified by D101 macroporous resin under the optimum conditions above, and the recovery rate was higher than 90%. The content of obtained flavonoids reached 62.6%, which was 2 times of the content before purification.
authors: Hubei Key Lab of Novel Reactor & Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, China.
authors: Shao-Wei
authors: SW
authors: Huang
authors: Ru-An
authors: RA
authors: Chi
authors: Yue-Fei
authors: YF
authors: Zhang
authors: Yuan-Xin
authors: YX
authors: Wu
authors: Xia-Lian
authors: XL
authors: Ju
authors: Jia
authors: J
authors: Guo
authors: Qi-Mao
authors: QM
authors: Huang
mesh_terms: D000327:Adsorption; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry*; D032281:Smilax / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012117:Resins, Synthetic