title: Expression of human transforming growth factor beta1 in E. coli (I) : Direct expression in cytoplasm.
abstract: PCR technique is used to amplify the mature peptide gene of human transforming growth factor pl (hTGFbeta1); the gene is verified by full-length sequence analysis. In DHSalpha/pBV220 expression system, hTGFbeta1 attains expression in the cytoplasm of E. coli up to 16%. The recombinant protein is proved to be the monomer of hTGFbetal by N-terminal amino acids analysis and immunoblotting. After refolding of the monomer protein in vitro in glutathione system or CHPAS/DMSO system, the dimeric protein accumulates to 30% in the refolding mixture. The recombinant protein is purified to homogeneity on silver staining, and is shown to have strong biological activity from MTT bioassay on MvlLu cells.
authors: Beijing Institute of Basic Medical Sciences, China.
authors: J
authors: J
authors: Long
authors: H
authors: H
authors: Wang
authors: L
authors: L
authors: Liu
authors: F
authors: F
authors: Wang
authors: T
authors: T
authors: Zhou