Degeneration of dystrophic or injured skeletal muscles induces high expression of Galectin-1.
Muscle degenerative diseases such as Duchenne Muscular Dystrophy are incurable and treatment options are still restrained. Understanding the mechanisms and factors responsible for muscle degeneration and regeneration will facilitate the development of novel therapeutics. Several recent studies have demonstrated that Galectin-1 (Gal-1), a carbohydrate-binding protein, induces myoblast differentiation and fusion in vitro, suggesting a potential role for this mammalian lectin in muscle regenerative processes in vivo. However, the expression and localization of Gal-1 in vivo during muscle injury and repair are unclear. We report the expression and localization of Gal-1 during degenerative-regenerative processes in vivo using two models of muscular dystrophy and muscle injury. Gal-1 expression increased significantly during muscle degeneration in the murine mdx and in the canine Golden Retriever Muscular Dystrophy animal models. Compulsory exercise of mdx mouse, which intensifies degeneration, also resulted in sustained Gal-1 levels. Furthermore, muscle injury of wild-type C57BL/6 mice, induced by BaCl(2) treatment, also resulted in a marked increase in Gal-1 levels. Increased Gal-1 levels appeared to localize both inside and outside the muscle fibers with significant extracellular Gal-1 colocalized with infiltrating CD45(+) leukocytes. By contrast, regenerating muscle tissue showed a marked decrease in Gal-1 to baseline levels. These results demonstrate significant regulation of Gal-1 expression in vivo and suggest a potential role for Gal-1 in muscle homeostasis and repair.
Cerri
Daniel G
DG
Muscular Dystrophy Research Center (AADM/UNAERP), School of Medicine, University of Ribeirão Preto, 14096-900 Ribeirão Preto-SP, Brazil.
Rodrigues
Lílian C
LC
Stowell
Sean R
SR
Araujo
Daniela D
DD
Coelho
Mariana C
MC
Oliveira
Sibere R
SR
Bizario
João C S
JC
Cummings
Richard D
RD
Dias-Baruffi
Marcelo
M
Costa
Maria Cristina R
MC
D000818:Animals; D004285:Dogs; D005260:Female; D005455:Fluorescent Antibody Technique; D037483:Galectin 1 / Q000032:analysis / Q000378:metabolism*; D017493:Leukocyte Common Antigens / Q000276:immunology / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000293:injuries* / Q000378:metabolism*; D009137:Muscular Dystrophy, Animal / Q000139:chemically induced / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology
D037483:Galectin 1; D017493:Leukocyte Common Antigens