title: [Isolation and physiological characteristics of lytic bacteriophages of Vibrio].
abstract: OBJECTIVE
  Vibrio is a widely distributed pathogen in aquatic environment. Our study aimed at searching for possible biological control of pathogenic vibrio.
  
  
  METHODS
  We collected natural samples from coast and lakes in spring of 2006 and autumn of 2005; and isolated lytic phages by double-layer plate method. We identified the hosts with 16S rDNA sequencing and observed their morphology with phages under electron microscopy. We also tested the physiological characteristics of phages.
  
  
  RESULTS
  We isolated 96 bacteria and 2 phages (Vibio/XM/P1, Vibio/XM/P2). Their hosts belonged to Vibrio alginolyticus and Vibrio anguillarum. Both phages were hexagonal-headed and one with a tail. Physiological tests show that their optimum grow condition were pH7, 25 degrees C and pH8, 30 degrees C. Both phages were sensitive to high temperature and UV light. Vibrio/XM/P2 was sensitive to aether and chloroform whereas Vibrio/XM/P1 not.
authors: Third Institute of Oceanography, Xiamen 361005, China. oscar_0406@163.com
authors: Jia
authors: J
authors: Sun
authors: Dezan
authors: D
authors: Ye
authors: Agnes
authors: A
authors: Kochel
authors: Guenter
authors: G
authors: Jost
mesh_terms: D001435:Bacteriophages / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity* / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002725:Chloroform / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004987:Ethers / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D013696:Temperature; D014466:Ultraviolet Rays / Q000009:adverse effects; D014733:Vibrio / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure / Q000821:virology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004987:Ethers; D002725:Chloroform