title: Decolorization of synthetic melanoidins-containing wastewater by a bacterial consortium.
abstract: The presence of melanoidins in molasses wastewater leads to water pollution both due to its dark brown color and its COD contents. In this study, a bacterial consortium isolated from waterfall sediment was tested for its decolorization. The identification of culturable bacteria by 16S rDNA based approach showed that the consortium composed of Klebsiella oxytoca, Serratia mercescens, Citrobacter sp. and unknown bacterium. In the context of academic study, prevention on the difficulties of providing effluent as well as its variations in compositions, several synthetic media prepared with respect to color and COD contents based on analysis of molasses wastewater, i.e., Viandox sauce (13.5% v/v), caramel (30% w/v), beet molasses wastewater (41.5% v/v) and sugarcane molasses wastewater (20% v/v) were used for decolorization using consortium with color removal 9.5, 1.13, 8.02 and 17.5%, respectively, within 2 days. However, Viandox sauce was retained for further study. The effect of initial pH and Viandox concentration on decolorization and growth of bacterial consortium were further determined. The highest decolorization of 18.3% was achieved at pH 4 after 2 day of incubation. Experiments on fresh or used medium and used or fresh bacterial cells, led to conclusion that the limitation of decolorization was due to nutritional deficiency. The effect of aeration on decolorization was also carried out in 2 L laboratory-scale suspended cell bioreactor. The maximum decolorization was 19.3% with aeration at KLa=2.5836 h(-1) (0.1 vvm).
authors: Biotechnology Program, Faculty of Science, Chulalongkorn University, Phyathai Road, Patumwan, Bangkok, 10330, Thailand.
authors: Suhuttaya
authors: S
authors: Jiranuntipon
authors: Supat
authors: S
authors: Chareonpornwattana
authors: Somsak
authors: S
authors: Damronglerd
authors: Claire
authors: C
authors: Albasi
authors: Marie-Line
authors: ML
authors: Delia
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D007220:Industrial Waste / Q000032:analysis*; D011108:Polymers / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D004396:Coloring Agents; D003470:Culture Media; D007220:Industrial Waste; D011108:Polymers; D014874:Water Pollutants, Chemical; C011908:melanoidin polymers