Determination of in vitro susceptibility of ocular Fusarium spp. isolates from keratitis cases and comparison of Clinical and Laboratory Standards Institute M38-A2 and E test methods.
We evaluated the susceptibility of 85 Fusarium spp. isolates from cases of fungal keratitis with 8 antifungal drugs using the standard Clinical and Laboratory Standards Institute broth microdilution and E test methods. Members of the Fusarium solani species complex showed consistently higher MICs to the triazole drugs itraconazole, voriconazole, and posaconazole than did members of other species complexes (Fusarium oxysporum and other minor species). High MICs to amphotericin B, natamycin, and echinocandins were consistently obtained with no discrimination based on species or method. Further work is required to determine any potential correlation between MIC and clinical outcome in keratitis.
Iqbal
Naureen J
NJ
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. NIqbal@cdc.gov
Boey
Angeline
A
Park
Benjamin J
BJ
Brandt
Mary E
ME
D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D015821:Eye Infections, Fungal / Q000188:drug therapy / Q000382:microbiology*; D005670:Fusarium / Q000187:drug effects*; D006801:Humans; D007634:Keratitis / Q000188:drug therapy / Q000382:microbiology*; D007753:Laboratories / Q000592:standards; D008826:Microbial Sensitivity Tests; D015203:Reproducibility of Results; D014230:Triazoles / Q000494:pharmacology* / Q000627:therapeutic use
D000935:Antifungal Agents; D014230:Triazoles