title: Different media and supplements modulate the clonogenic and expansion properties of rabbit bone marrow mesenchymal stem cells.
abstract: BACKGROUND
  Rabbits provide an excellent model for many animal and human diseases, such as cardiovascular diseases, for the development of new vaccines in wound healing management and in the field of tissue engineering of tendon, cartilage, bone and skin.The study presented herein aims to investigate the biological properties of bone marrow rabbit MSCs cultured in different conditions, in order to provide a basis for their clinical applications in veterinary medicine.
  
  
  RESULTS
  MSCs were isolated from 5 New Zealand rabbits. Fold increase, CFU number, doubling time, differentiation ability and immunophenotype were analyzed.With the plating density of 10 cells/cm2 the fold increase was significantly lower with DMEM-20%FCS and MSCs growth was significantly higher with alphaMEM-hEGF. The highest clonogenic ability was found at 100 cell/cm2 with MSCBM and at 10 cell/cm2 with M199. Both at 10 and 100 cells/cm2, in alphaMEM medium, the highest CFU increase was obtained by adding bFGF. Supplementing culture media with 10%FCS-10%HS determined a significant increase of CFU.
  
  
  CONCLUSIONS
  Our data suggest that different progenitor cells with differential sensitivity to media, sera and growth factors exist and the choice of culture conditions has to be carefully considered for MSC management.
authors: Cell Biology and Tissue Regeneration Laboratory, University of Pisa, Italy. s.lapi@ao-pisa.toscana.it
authors: Simone
authors: S
authors: Lapi
authors: Francesca
authors: F
authors: Nocchi
authors: Roberta
authors: R
authors: Lamanna
authors: Simona
authors: S
authors: Passeri
authors: Mariacarla
authors: M
authors: Iorio
authors: Aldo
authors: A
authors: Paolicchi
authors: Patrizia
authors: P
authors: Urciuoli
authors: Alessandra
authors: A
authors: Coli
authors: Francesca
authors: F
authors: Abramo
authors: Vincenzo
authors: V
authors: Miragliotta
authors: Elisabetta
authors: E
authors: Giannessi
authors: Maria Rita
authors: MR
authors: Stornelli
authors: Renato
authors: R
authors: Vanacore
authors: Giulia
authors: G
authors: Stampacchia
authors: Guido
authors: G
authors: Pisani
authors: Luciano
authors: L
authors: Borghetti
authors: Fabrizio
authors: F
authors: Scatena