title: On the mechanism of quinol oxidation at the QP site in the cytochrome bc1 complex: studied using mutants lacking cytochrome bL or bH.
abstract: To elucidate the mechanism of bifurcated oxidation of quinol in the cytochrome bc1 complex, Rhodobacter sphaeroides mutants, H198N and H111N, lacking heme bL and heme bH, respectively, were constructed and characterized. Purified mutant complexes have the same subunit composition as that of the wild-type complex, but have only 9-11% of the electron transfer activity, which is sensitive to stigmatellin or myxothiazol. The Em values for hemes bL and bH in the H111N and H198N complexes are -95 and -35 mV, respectively. The pseudo first-order reduction rate constants for hemes bL and bH in H111N and H198N, by ubiquiniol, are 16.3 and 12.4 s(-1), respectively. These indicate that the Qp site in the H111N mutant complex is similar to that in the wild-type complex. Pre-steady state reduction rates of heme c1 by these two mutant complexes decrease to a similar extent of their activity, suggesting that the decrease in electron transfer activity is due to impairment of movement of the head domain of reduced iron-sulfur protein, caused by disruption of electron transfer from heme bL to heme bH. Both mutant complexes produce as much superoxide as does antimycin A-treated wild-type complex. Ascorbate eliminates all superoxide generating activity in the intact or antimycin inhibited wild-type or mutant complexes.
authors: Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
authors: Shaoqing
authors: S
authors: Yang
authors: He-Wen
authors: HW
authors: Ma
authors: Linda
authors: L
authors: Yu
authors: Chang-An
authors: CA
authors: Yu
mesh_terms: D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives / Q000737:chemistry; D003580:Cytochromes / Q000378:metabolism; D045303:Cytochromes b / Q000737:chemistry* / Q000235:genetics; D004579:Electron Transport; D014450:Electron Transport Complex III / Q000737:chemistry*; D006418:Heme / Q000737:chemistry; D006736:Horses; D006873:Hydroquinones / Q000737:chemistry*; D007700:Kinetics; D008954:Models, Biological; D009154:Mutation; D010084:Oxidation-Reduction; D011485:Protein Binding; D012242:Rhodobacter sphaeroides / Q000235:genetics* / Q000378:metabolism
chemical_list: D003580:Cytochromes; D006873:Hydroquinones; C032456:antimycin; D006418:Heme; D000968:Antimycin A; D045303:Cytochromes b; D014450:Electron Transport Complex III