Dark quenched matrix metalloproteinase fluorogenic probe for imaging osteoarthritis development in vivo.
The early detection of osteoarthritis (OA) is currently a key challenge in the field of rheumatology. Biochemical studies of OA have indicated that matrix metalloproteinase-13 (MMP-13) plays a central role in cartilage degradation. In this study, we describe the potential use of a dark-quenched fluorogenic MMP-13 probe to image MMP-13 in both in vitro and rat models. The imaging technique involved using a MMP-13 peptide substrate, near-infrared (NIR) dye, and a NIR dark quencher. The results from this study demonstrate that the use of a dark-quenched fluorogenic probe allows for the visual detection of MMP-13 in vitro and in OA-induced rat models. In particular, by targeting this OA biomarker, the symptoms of the early and late stages of OA can be readily monitored, imaged, and analyzed in a rapid and efficient fashion. We anticipate that this simple and highly efficient fluorogenic probe will assist in the clinical management of patients with OA, not only for early diagnosis but also to assess individual patient responses to new drug treatments.
Lee
Seulki
S
Biomedical Research Center, Korea Institute of Science and Technology, Seongbuk-Gu, Seoul, South Korea.
Park
Kyeongsoon
K
Lee
Seung-Young
SY
Ryu
Ju Hee
JH
Park
Jong Woong
JW
Ahn
Hyung Jun
HJ
Kwon
Ick Chan
IC
Youn
In-Chan
IC
Kim
Kwangmeyung
K
Choi
Kuiwon
K
D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology*; D003952:Diagnostic Imaging / Q000379:methods*; D005456:Fluorescent Dyes; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D010003:Osteoarthritis / Q000201:enzymology / Q000473:pathology*; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
D005456:Fluorescent Dyes; D053509:Matrix Metalloproteinase 13