title: Evidence for bacteriophage T7 tail extension during DNA injection.
abstract: BACKGROUND
  Electron micrographs of bacteriophage T7 reveal a tail shorter than needed to reach host cytoplasm during infection-initiating injection of a T7 DNA molecule through the tail and cell envelope. However, recent data indicate that internal T7 proteins are injected before the DNA molecule is injected. Thus, bacteriophage/host adsorption potentially causes internal proteins to become external and lengthen the tail for DNA injection. But, the proposed adsorption-induced tail lengthening has never been visualized.
  
  
  RESULTS
  In the present study, electron microscopy of particles in T7 lysates reveals a needle-like capsid extension that attaches partially emptied bacteriophage T7 capsids to non-capsid vesicles and sometimes enters an attached vesicle. This extension is 40-55 nm long, 1.7-2.4x longer than the T7 tail and likely to be the proposed lengthened tail. The extension is 8-11 nm in diameter, thinner than most of the tail, with an axial hole 3-4 nm in diameter. Though the bound vesicles are not identified by microscopy, these vesicles resemble the major vesicles in T7 lysates, found to be E. coli outer membrane vesicles by non-denaturing agarose gel electrophoresis, followed by mass spectrometry.
  
  
  CONCLUSIONS
  The observed lengthened tail is long enough to reach host cytoplasm during DNA injection. Its channel is wide enough to be a conduit for DNA injection and narrow enough to clamp DNA during a previously observed stalling/re-starting of injection. However, its outer diameter is too large to explain formation by passing of an intact assembly through any known capsid hole unless the hole is widened.
authors: Department of Biochemistry, The University of Texas Health Science Center, San Antonio, Texas 78229-3900, USA. serwer@uthscsa.edu
authors: Philip
authors: P
authors: Serwer
authors: Elena T
authors: ET
authors: Wright
authors: Kevin W
authors: KW
authors: Hakala
authors: Susan T
authors: ST
authors: Weintraub