title: X-ray induced damage in DNA monitored by X-ray photoelectron spectroscopy.
abstract: In this work, the chemical changes in calf thymus DNA samples were analyzed by X-ray photoelectron spectroscopy (XPS). The DNA samples were irradiated for over 5 h and spectra were taken repeatedly every 30 min. In this approach the X-ray beam both damages and probes the samples. In most cases, XPS spectra have complex shapes due to contributions of C, N, and O atoms bonded at several different sites. We show that from a comparative analysis of the modification in XPS line shapes of the C 1s, O 1s, N 1s, and P 2p peaks, one can gain insight into a number of reaction pathways leading to radiation damage to DNA.
authors: Department of Physics and Astronomy, The Open University, Walton Hall, Milton Keynes, MK7 6AA, United Kingdom. s.ptasinska@open.ac.uk
authors: Sylwia
authors: S
authors: Ptasińska
authors: Agnieszka
authors: A
authors: Stypczyńska
authors: Tony
authors: T
authors: Nixon
authors: Nigel J
authors: NJ
authors: Mason
authors: Dimitri V
authors: DV
authors: Klyachko
authors: Léon
authors: L
authors: Sanche
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000235:genetics; D004249:DNA Damage / Q000528:radiation effects*; D013057:Spectrum Analysis; D014965:X-Rays
chemical_list: D004247:DNA; C427203:calf thymus DNA