Wnt4 and Wnt5a promote adipocyte differentiation.
The roles of the non-canonical Wnt pathway during adipogenesis are not well known, though Wnt10b is known to function as a negative regulator for adipogenesis by activating the canonical Wnt pathway. We focused on the roles of Wnt4, Wnt5a and Wnt6, which are thought to be part of the non-canonical Wnt pathway. The expression of these genes changed dramatically at the initial stage of adipogenesis. Furthermore, the inhibition of Wnt4 or Wnt5a expression prevented the accumulation of triacylglycerol and decreased the expression of adipogenesis-related genes. Wnt4 and Wnt5a have crucial roles in adipogenesis as positive regulators.
Nishizuka
Makoto
M
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Nagoya, Aichi 467-8603, Japan.
Koyanagi
Akiko
A
Osada
Shigehiro
S
Imagawa
Masayoshi
M
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D005786:Gene Expression Regulation; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D014280:Triglycerides / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics / Q000502:physiology*; D000071818:Wnt-5a Protein; D060528:Wnt4 Protein
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D014280:Triglycerides; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; D060528:Wnt4 Protein; C555582:Wnt4 protein, mouse; C438615:Wnt5a protein, mouse; C095936:Wnt6 protein, mouse