Isolation and characterization of multipotent human keloid-derived mesenchymal-like stem cells.
In this study, we report the isolation and characterization of a population of multipotent keloid-derived mesenchymal-like stem cells (KMLSCs) from keloid scalp tissues. These KMLSCs expressed the typical mesenchymal stem cell marker proteins CD13, CD29, CD44, CD90, fibronectin, and vimentin when they were cultured in serum-containing medium and when subsequent exposure to various differentiation media resulted in their differentiation into adipocytes, osteoblasts, chondrocytes, smooth muscle cells, and angiogenic endothelial cells. When KMLSCs were cultured in neural stem culture conditions (i.e., in the presence of epidermal growth factor and fibroblast growth factor 2 in substrate-free conditions), they produced large numbers of neurospheres containing nestin-, CD133-, and SOX2-positive cells that expressed neural-crest stem cell markers. Subsequent exposure of these cells to different differentiation conditions resulted in cells that expressed neuronal cell-, astrocyte-, oligodendrocyte-, or Schwann cell-specific markers. Our study suggests that KMLSCs may be an alternative adult stem cell resource for regenerative tissue repair and auto-transplantation.
Moon
Jai-Hee
JH
The Laboratory of Cell Growth and Function Regulation, College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
Kwak
Sung Sik
SS
Park
Gyuman
G
Jung
Hye-Youn
HY
Yoon
Byung Sun
BS
Park
Jaeyeo
J
Ryu
Kyung Su
KS
Choi
Seung-Cheol
SC
Maeng
Isaac
I
Kim
Bona
B
Jun
Eun Kyung
EK
Kim
Soonseong
S
Kim
Aeree
A
Oh
Sejong
S
Kim
Hyunggee
H
Kim
Ki Dong
KD
You
Seungkwon
S
D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D015703:Antigens, CD / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007627:Keloid; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009417:Nerve Tissue / Q000166:cytology / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology / Q000378:metabolism; D012038:Regeneration; D014182:Transplantation, Autologous
D015703:Antigens, CD; D003470:Culture Media; D016207:Cytokines