[Effect of total flavonoid in leaves of Ginkgo biloba on the apoptosis of eosinophil in broncho alveloar lavage fluid].
This study was to investigate the effect of total flavonoid in leaves of Ginkgo biloba (total flavonoid in leaves of Ginkgo biloba, FG) on the apoptosis of eosinophils (EOS) in broncho alveloar lavage fluid (BALF) of asthma mice. Mouse asthma model was established by ovalbumin (OVA) challenge methods. After atomizing therapy for two weeks, differential count in BALF, morphological change and proportion of apoptosis were detected by AO/EB stain and Annexin V-FITC/PI. The number of total leucocytes and eosinophils in BALF decreased obviously after FG treatment. Compared with model group, the number and proportion of EOS apoptosis increased significantly after FG treatment. The results indicated that one of the anti-inflammation mechanisms of FG might be promoting apoptosis of eosinophils.
Weng
Xiao-Jing
XJ
Medical and Pharmaceutical Institute, Yangzhou University, Yangzhou 225001, China.
Chen
Li-Li
LL
Zhang
Hong-Quan
HQ
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001249:Asthma / Q000139:chemically induced / Q000473:pathology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004804:Eosinophils / Q000473:pathology*; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry; D007958:Leukocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D018515:Plant Leaves / Q000737:chemistry; D011897:Random Allocation
D000894:Anti-Inflammatory Agents, Non-Steroidal; D005419:Flavonoids; D010047:Ovalbumin