Nanoscale-enhanced Ru(bpy)3(2+) electrochemiluminescence labels and related aptamer-based biosensing system.
A unique multilabeling at a single-site protocol of the Ru(bpy)(3)(2+) electrochemiluminescence (ECL) system is proposed. Nanoparticles (NPs) were used as assembly substrates to enrich ECL co-reactants of Ru(bpy)(3)(2+) to construct nanoscale-enhanced ECL labels. Two different kinds of NP substrates [including semiconductor NPs (CdTe) and noble metal NPs (gold)] capped with 2-(dimethylamino)ethanethiol (DMAET) [a tertiary amine derivative which is believed to be one of the most efficient of co-reactants of the Ru(bpy)(3)(2+) system] were synthesized through a simple one-pot synthesis method in aqueous media. Although both CdTe and gold NPs realized the enrichment of ECL co-reactants, they presented entirely different ECL performances as nanoscale ECL co-reactants of Ru(bpy)(3)(2+). The different effects of these two NPs on the ECL of Ru(bpy)(3)(2+) were studied. DMAET-capped CdTe NPs showed enormous signal amplification of Ru(bpy)(3)(2+) ECL, whereas DMAET-capped gold NPs showed a slight quenching effect of the ECL signal. DMAET-capped CdTe NPs can be considered to be excellent nanoscale ECL labels of the Ru(bpy)(3)(2+) system, as even a NP solution sample of 10(-18) M was still detectable after an electrostatic self-assembly concentration process. DMAET-capped CdTe NPs were further applied in the construction of aptamer-based biosensing system for proteins and encouraging results were obtained.
Guo
Weiwei
W
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Yuan
Jipei
J
Li
Bingling
B
Du
Yan
Y
Ying
Erbo
E
Wang
Erkang
E
D052158:Aptamers, Peptide; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D053758:Nanoparticles; D012428:Ruthenium
D052158:Aptamers, Peptide; D012428:Ruthenium