Development of a transposon mutagenesis system in the oral spirochete Treponema denticola.
Here, we report successful transposon mutagenesis in the oral spirochete Treponema denticola. A modified Himar1 transposon, including a new antibiotic selection cassette for T. denticola, generated mutations affecting cell division, transport, and chemotaxis, among other processes. This random mutagenesis system should facilitate research on the biology and pathogenesis of this spirochete, which is associated with human periodontal diseases.
Yang
Yu
Y
Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214, 14214-3092, USA.
Stewart
Philip E
PE
Shi
Xiaoguang
X
Li
Chunhao
C
D004251:DNA Transposable Elements; D024881:Drug Resistance, Bacterial; D006801:Humans; D016254:Mutagenesis, Insertional / Q000379:methods*; D010510:Periodontal Diseases / Q000382:microbiology; D012641:Selection, Genetic; D048373:Treponema denticola / Q000235:genetics*
D004251:DNA Transposable Elements