hOLFML1, a novel secreted glycoprotein, enhances the proliferation of human cancer cell lines in vitro.
We describe a novel secreted protein, named hOLFML1 (human olfactomedin-like protein 1), with an olfectamine domain in its C-terminus, mainly expressed in the small intestine, liver, lung and heart. Immunohistochemical staining on human small intestine indicated that the protein localizes preferentially in the intestinal villi. Interestingly, ectopic hOLFML1 promoted proliferation of HeLa cells and increased the percentage of cells in S phase. In contrast, knock down of hOLFML1 protein expression by siRNA inhibited cell proliferation and delayed the entry of cells into S phase. Our data also revealed that hOLFML1 is N-glycosylated and its secretion is triggered by serum. Taken together, these findings suggest that hOLFML1 may play a significant role in the regulation of cell proliferation in vitro.
Wan
Bingbing
B
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University and Chinese National Human Genome Center at Shanghai, 351 Guo Shou-Jing Road, Shanghai 201203, China.
Zhou
Yu-Bo
YB
Zhang
Xin
X
Zhu
Hong
H
Huo
Keke
K
Han
Ze-Guang
ZG
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D016326:Extracellular Matrix Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006023:Glycoproteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism; D010802:Phylogeny; D034741:RNA, Small Interfering / Q000235:genetics; D014018:Tissue Distribution
D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D034741:RNA, Small Interfering; C081451:olfactomedin