title: Efficacy of culture filtrates of Metarhizium anisopliae against larvae of Anopheles stephensi and Culex quinquefasciatus.
abstract: Efficacy of culture filtrates of five strains of Metarhizium anisopliae isolated from insects were evaluated against Anopheles stephensi and Culex quinquefasciatus. The culture filtrates released from the strains of M. anisopliae in the YpSs and chitin broths were filtered and used for the bioassays after a growth of 7 days. Among the culture filtrates of five strains, M. anisopliae 892 was found to be more effective against both the mosquitoes. The LC(50) values of culture filtrates of M. anisopliae 892 in chitin broth was lower than the LC(50) of culture filtrates in YpSs broth against first and fourth instars of both the mosquitoes. The LC(50) values of culture filtrates were significantly different between first and fourth instars of A. stephensi (t test; P = 0.0001) and C. quinquefasciatus (t test; P = 0.02). The larvae of A. stephensi were more susceptible than C. quinquefasciatus except in two cases. This is the first report of efficacy of culture filtrates produced by M. anisopliae in chitin broth against mosquitoes and have potential as a biological control agent of mosquitoes.
authors: National Institute of Malaria Research, Indian Council of Medical Research, 22-Sham Nath Marg, Delhi, India. ssnimr@gmail.com
authors: Suman Sundar
authors: SS
authors: Mohanty
authors: Kamaraju
authors: K
authors: Raghavendra
authors: Pradeep Kumar
authors: PK
authors: Mittal
authors: Aditya Prasad
authors: AP
authors: Dash
mesh_terms: D000818:Animals; D000852:Anopheles / Q000187:drug effects*; D003465:Culex / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D052981:Metarhizium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D003470:Culture Media