title: Characters of DNA constitution in the rye B chromosome.
abstract: We have used chromosome microdissection and microcloning to construct a DNA library of the entire B chromosome (B) of rye. New rye B-specific sequences have been screened from this pool, blasted with other sequences and analyzed to elucidate the characters of DNA constitution and the possible pathway of the origin of the rye B chromosome. We report the discovery of a new sequence that is specific to the rye B centromere.
authors: College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Hong
authors: H
authors: Long
authors: Zhong-Xia
authors: ZX
authors: Qi
authors: Xiao-Ming
authors: XM
authors: Sun
authors: Cheng-Bin
authors: CB
authors: Chen
authors: Xiu-Lan
authors: XL
authors: Li
authors: Wen-Qin
authors: WQ
authors: Song
authors: Rui-Yang
authors: RY
authors: Chen
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D018628:Gene Dosage; D042282:Microdissection; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012434:Secale / Q000235:genetics*
chemical_list: D018744:DNA, Plant