title: Infiltration of nestin-expressing cells in interstitial fibrosis in chronic cyclosporine nephropathy.
abstract: BACKGROUND
  Nestin-expressing cells play a role in the repair process of injured tissues and organs. This study examined the nestin-expressing cells in interstitial fibrosis in experimental chronic cyclosporine A (CsA) nephropathy.
  
  
  METHODS
  Sprague Dawley rats were treated daily for 1 or 4 weeks with CsA (15 mg/kg) or vehicle (VH; olive oil, 1 mg/kg). Nestin mRNA expression was evaluated with reverse transcriptional-polymerase chain reaction, and nestin-expressing cells were detected immunohistochemically. Localization of nestin was performed with double labeling studies for vimentin, aquaporin 1, or calbindin D28K.
  
  
  RESULTS
  Nestin mRNA expression was not different between VH- and CsA-treated rat kidneys. Nestin-expressing cells were rarely observed in the cortex in the VH group, but CsA-induced renal injury caused an increase in nestin-expressing cells in the cortex in a time-dependent manner. Nestin-expressing cells in the CsA group were localized to the area of interstitial fibrosis, and the number of nestin-expressing cells well correlated with the score of interstitial fibrosis (r=0.898). Nestin-expressing cells did not express vimentin, aquaporin 1, or calbindin D28K.
  
  
  CONCLUSIONS
  CsA-induced renal injury recruits nestin-expressing cells to injured areas, and these cells might be involved in reparative fibrosis in the progression of chronic CsA nephropathy.
authors: Division of Nephrology, Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Kyung Ohk
authors: KO
authors: Ahn
authors: Can
authors: C
authors: Li
authors: Sun Woo
authors: SW
authors: Lim
authors: Hyun Kuk
authors: HK
authors: Song
authors: Jung Yeon
authors: JY
authors: Ghee
authors: Su Hyun
authors: SH
authors: Kim
authors: Jin Young
authors: JY
authors: Kim
authors: Hye Eun
authors: HE
authors: Yoon
authors: Jung Ho
authors: JH
authors: Cha
authors: Jin
authors: J
authors: Kim
authors: Chul Woo
authors: CW
authors: Yang
mesh_terms: D016572:Cyclosporine / Q000633:toxicity*; D018856:Cystitis, Interstitial / Q000139:chemically induced / Q000235:genetics; D006801:Humans; D007166:Immunosuppressive Agents / Q000633:toxicity; D007381:Intermediate Filament Proteins / Q000235:genetics*; D007668:Kidney / Q000187:drug effects / Q000473:pathology*; D007674:Kidney Diseases / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D064231:Nestin; D009926:Organ Preservation / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007166:Immunosuppressive Agents; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D012333:RNA, Messenger; D016572:Cyclosporine