Expression profiling of supraglottic carcinoma: PTEN and thrombospondin 2 are associated with inhibition of lymphatic metastasis.
CONCLUSIONS
Down-regulation of metastasis inhibitor genes PTEN and thrombospondin 2 may play a role in the lymphatic metastasis of supraglottic carcinoma.


OBJECTIVE
To investigate differentially expressed genes in supraglottic squamous cell carcinoma with and without lymphatic metastasis using cDNA microarray.


METHODS
cDNA microarray was used to detect the expression profiles of supraglottic carcinoma and the results were validated with real-time PCR.


RESULTS
Compared with normal laryngeal tissues, the expression of 15.6% (15/96) genes including cathepsin B, cathepsin D, cathepsin L, Ets1, c-Ets2, and PEA3 were up-regulated in both cases of supraglottic carcinoma. Compared with the supraglottic carcinoma with no lymph node metastasis, the expression of metastasis inhibitor genes PTEN and thrombospondin 2 was down-regulated in the supraglottic carcinoma tissue with lymph node metastasis.
Bai
Weiliang
W
Department of Otorhinolaryngology, Shenjing Hospital of China Medical University, Shenyang, China. bweiliang@yahoo.com.cn
Wang
Liping
L
Ji
Wenyue
W
Gao
Hong
H
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008207:Lymphatic Metastasis / Q000235:genetics / Q000517:prevention & control; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D019699:Thrombospondins / Q000378:metabolism*; D000099262:Thrombospondin 2
D051059:PTEN Phosphohydrolase; D019699:Thrombospondins; D000099262:Thrombospondin 2; C494929:PTEN protein, human