[Determination of melamine residue in feeds by ultra performance liquid chromatography coupled with electrospray tandem mass spectrometry].
A method for fast determination melamine residue in feeds was established. An ultra performance liquid chromatography coupled with electrospray ionization quadrupole tandem mass spectrometry (UPLC-ESI-MS/MS) was used. The residue was quantified with multiple reaction monitoring (MRM) mode. The method was validated and good results were obtained with respect to precision, repeatability and spiked recovery. The limit of detection was 10 microg/kg for melamine, and the linear range was from 10 to 5 000 1g/kg. The average recoveries were between 83% and 94% in the spiked range of 10 - 100 microg/kg, and the relative standard deviations (RSDs) were between 4.2% and 6.5%. The method has good repeatability and high sensitivity, and can be applied for the determination of melamine residue in feeds.
Cai
Qinren
Q
Technical Center of Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519015, China. mr.cai@vip.163.com
Ouyang
Yingyu
Y
Qian
Zhenjie
Z
Peng
Yufen
Y
D000821:Animal Feed / Q000032:analysis* / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D011786:Quality Control; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013997:Time Factors; D014227:Triazines / Q000032:analysis*
D014227:Triazines; C011907:melamine