title: Nonlinear immunofluorescent assay for androgenic hormones based on resonant structures.
abstract: We report for the first time the use of two photon fluorescence as detection method of affinity binding reactions. We use a resonant grating waveguide structure as platform enhancement for detecting the interaction between fluorescent labeled Boldenone, a non-natural androgenic hormone, and a specific anti-anabolic antibody. We were able to detect a surface coverage of approximately 0.7 ng/mm(2).
authors: ICFO-Institut de Ciencies Fotoniques, Ada Canal Olimpic s/n, 08600 Castelldefels, Barcelona, Spain.
authors: Anisha
authors: A
authors: Thayil
authors: Alejandro
authors: A
authors: Muriano
authors: J-Pablo
authors: JP
authors: Salvador
authors: Roger
authors: R
authors: Galve
authors: Maria P
authors: MP
authors: Marco
authors: Dobryna
authors: D
authors: Zalvidea
authors: Pablo
authors: P
authors: Loza-Alvarez
authors: Tsvi
authors: T
authors: Katchalski
authors: Eran
authors: E
authors: Grinvald
authors: Asher A
authors: AA
authors: Friesem
authors: Silvia
authors: S
authors: Soria
mesh_terms: D000728:Androgens / Q000032:analysis*; D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D017711:Nonlinear Dynamics; D010783:Photometry / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D014159:Transducers
chemical_list: D000728:Androgens