Toward a full characterization of native starch: separation and detection by size-exclusion chromatography.
The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 degrees C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer.
Hoang
Ngoc-Ly
NL
University of Queensland, CNAFS/SLCAFS, Hartley Teakle Building 83, Brisbane , QLD 4072, Australia.
Landolfi
Antonin
A
Kravchuk
Anastasiya
A
Girard
Etienne
E
Peate
Jonathan
J
Hernandez
Javier M
JM
Gaborieau
Marianne
M
Kravchuk
Olena
O
Gilbert
Robert G
RG
Guillaneuf
Yohann
Y
Castignolles
Patrice
P
D019840:2-Propanol / Q000737:chemistry; D000081:Acetamides; D001965:Bromides; D002850:Chromatography, Gel / Q000379:methods*; D004121:Dimethyl Sulfoxide; D018020:Lithium Compounds; D011142:Polyvinyl Alcohol / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013213:Starch / Q000031:analogs & derivatives / Q000737:chemistry* / Q000302:isolation & purification*
D000081:Acetamides; D001965:Bromides; D018020:Lithium Compounds; C040949:lithium bromide; D011142:Polyvinyl Alcohol; D013213:Starch; C013959:dimethylacetamide; D019840:2-Propanol; D004121:Dimethyl Sulfoxide