[Mechanism of ligustrazine promoting hematopoietic reconstitution in syngenic bone marrow transplanted mice].
The objective of this study was to investigate the effect of ligustrazine on the expression of stem cell factor mRNA (SCF) in bone marrow tissue and explore the mechanism of hematopoietic reconstitution after bone marrow transplantation (BMT). The colony forming unit of spleen (CFU-S) were counted, the survival rate at days 7, 14 and 21 after BMT were measured, as well as the expression level of SCF mRNA was detected by RT-PCR. The results showed that in ligustrazine group CFU-S counts on day 10 and survival rate, expression level of SCF mRNA on day 7, 14 and 21 after BMT were higher than that in the control group (p < 0.01 or p < 0.05). In conclusion, ligustrazine promotes the recovery of hematopoietic cells in bone marrow, enhances the repair of bone marrow microvessels, and then improves bone marrow microenvironment and promotes hematopoietic reconstitution.
He
Li
L
Department of Hematology, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China.
Sun
Han-Ying
HY
Zhang
Ke-Jian
KJ
Zuo
Xue-Lan
XL
Tan
Xi-You
XY
D000818:Animals; D016026:Bone Marrow Transplantation; D006410:Hematopoiesis / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011719:Pyrazines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019089:Stem Cell Factor / Q000235:genetics / Q000378:metabolism; D014185:Transplantation, Isogeneic
D011719:Pyrazines; D012333:RNA, Messenger; D019089:Stem Cell Factor; C017953:tetramethylpyrazine