[Application of thrombelastography in evaluation of platelet function during storage].
This study was aimed to explore changes of platelet function in vitro during storage by thrombelastography (TEG). 12 units plateletpheresis were randomly selected and stored at 20 to 24 degrees C with agitation. Thrombelastography variable parameters R, K values and maximal amplitude (MA) were measured on 1, 2, 3, 4, 5 days of platelet storage. Platelet concentration, mean platelet volume (MPV), hypotonic shock response (HSR), CD62p expression and CD62p reexpression on platelet surface were detected at the same time. Changes of platelet function in virto were systematically evaluated by above-mentioned indexes. The results showed that MPV augmented slightly with prolongation of preserved time (p > 0.05), and CD62p expression on platelet surface increased remarkably (p < 0.01), while CD62p reexpression decreased gradually (p < 0.01). There were no significant differences in HSR level of platelets during storage (p > 0.05). R value increased with prolongation of preserved time (p < 0.01). There were no obvious changes on K value and alpha Angle during storage (p > 0.05). There were no obvious changes in MA from 1 to 4 days, and MA decreased slightly on day 5 (p < 0.05). It is concluded that there was no significant change in MA and HSR which reflects comprehensive coagulation of platelets during storage. Platelets on the end of storage have excellent function of hemostasis; Thrombelastography parameter MA value can be used as a valuable indicator for evaluation of platelet function in vitro during storage.
Yu
Yang
Y
Department of Blood Transfusion, Center for Clinical Transfusion Medicine, PLA General Hospital, Beijing 100853, China.
Lin
Zi-Lin
ZL
Fen
Qian
Q
Pan
Ji-Chun
JC
Zhang
Ting
T
Sun
Gui-Xiang
GX
Zhang
Xiao-Juan
XJ
Ma
Chun-Ya
CY
Ge
Guo-Feng
GF
Wang
De-Qing
DQ
Luo
Qun
Q
Tian
Ya-Ping
YP
D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation; D006801:Humans; D010979:Platelet Function Tests / Q000379:methods; D013916:Thrombelastography