title: Trade-off between iron uptake and protection against oxidative stress: deletion of cueO promotes uropathogenic Escherichia coli virulence in a mouse model of urinary tract infection.
abstract: The periplasmic multicopper oxidase (CueO) is involved in copper homeostasis and protection against oxidative stress. Here, we show that the deletion of cueO in uropathogenic Escherichia coli increases its colonization of the urinary tract despite its increased sensitivity to hydrogen peroxide. The cueO deletion mutant accumulated iron with increased efficiency compared to its parent strain; this may account for its advantage in the iron-limited environment of the urinary tract.
authors: School of Molecular and Microbial Sciences, University of Queensland, Brisbane 4072, Australia.
authors: Jai J
authors: JJ
authors: Tree
authors: Glen C
authors: GC
authors: Ulett
authors: Cheryl-Lynn Y
authors: CL
authors: Ong
authors: Darren J
authors: DJ
authors: Trott
authors: Alastair G
authors: AG
authors: McEwan
authors: Mark A
authors: MA
authors: Schembri
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D017353:Gene Deletion; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D050296:Microbial Viability; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D001743:Urinary Bladder / Q000382:microbiology; D014552:Urinary Tract Infections / Q000382:microbiology*; D014556:Urine / Q000382:microbiology; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D006861:Hydrogen Peroxide; D007501:Iron; D010088:Oxidoreductases; C499679:cueO protein, E coli