Expression of mouse 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 mRNA alternative splice variants in hypoxia.
Expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB-3) mRNA alternative splice variants was studied in different mouse tissues in hypoxic conditions in vivo. Significant increase of the expression of PFKFB-3 mRNA was observed in the mouse lungs, testes and brain in hypoxia. Several new alternative splice variants of PFKFB-3 mRNA were identified in the lung, testis, brain and skeletal muscle. They have different length and amino acid sequence of C-terminal regulatory part. However, 6-phosphofructo-2-kinase and fructose-2,6-bisphosphatase catalytic domains were identical. Moreover, the expression of different alternative splice variants of PFKFB-3 mRNA has shown tissue specificity and different levels of induction in hypoxic conditions in vivo. Results of this investigation indicate a possible role of PFKFB-3 splice isoform in cell adaptation to hypoxic conditions.
Mykhalchenko
V G
VG
Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv, Ukraine.
Minchenko
D O
DO
Tsuchihara
K
K
Moenner
M
M
Komisarenko
S V
SV
Bikfalvi
A
A
Esumi
H
H
Minchenko
O H
OH
D000222:Adaptation, Physiological / Q000235:genetics*; D017398:Alternative Splicing / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D015870:Gene Expression / Q000502:physiology; D000860:Hypoxia / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology; D007527:Isoenzymes / Q000235:genetics; D008168:Lung / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology; D025481:Phosphofructokinase-2 / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000201:enzymology
D007527:Isoenzymes; D012333:RNA, Messenger; D025481:Phosphofructokinase-2