title: Comparison of cellular proliferation on dense and porous PCL scaffolds.
abstract: In this contribution, PCL (poly-epsilon caprolactone) scaffolds were prepared by solvent-casting/particle-leaching technique in the presence of two pore formers, PEG(4000) or sucrose molecules in different quantities (0, 10, 20, 30, 40, 50, 55 w/w% PEG(4000)/PCL; 10, 20 w/w% Sucrose/PCL). The surface and bulk properties of the resulting scaffolds were studied by SEM, DSC and FTIR. SEM photographs showed that, macroporosity was obtained in the PCL structures prepared with sucrose crystals while microporous structure was obtained in the presence of PEG(4000) molecules. Average pore diameters calculated from SEM photographs were 40.1 and 191.2 mum for 40% PEG(4000)/PCL and 10% Sucrose/PCL scaffolds, respectively. The DSC and FTIR results confirmed that there is no any interaction between pore formers and PCL during structural formation, and both pore formers, PEG(4000) and sucrose, remained independently in the scaffolds. L929 mouse fibroblast cells were seeded onto PCL structures and maintained during 7 days to evaluate cell proliferation. Cell culture results showed that, 10% Sucrose/PCL scaffold was the most promising substrate for L929 cell growth due to 3-D architecture and macroporous structure of the scaffold.
authors: Department of Materials Engineering, Atilim University, Ankara, Turkey.
authors: Hilal Türkoğlu
authors: HT
authors: Saşmazel
authors: Menemşe
authors: M
authors: Gümüşderelioğlu
authors: Aylin
authors: A
authors: Gürpinar
authors: Mehmet Ali
authors: MA
authors: Onur
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D002460:Cell Line; D049109:Cell Proliferation; D005347:Fibroblasts / Q000166:cytology; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone