title: Markers to evaluate the quality and self-renewing potential of engineered human skin substitutes in vitro and after transplantation.
abstract: We screened a series of antibodies for their exclusive binding to the human hair follicle bulge. In a second step these antibodies were to be used to identify basal keratinocytes and potential epithelial stem cells in the human epidermis and in engineered skin substitutes. Of all the antibodies screened, we identified only one, designated C8/144B, that exclusively recognized the hair follicle bulge. However, C8/144B-binding cells were never detected in the human epidermal stratum basale. In the bulge C8/144B-binding cells gave rise to cytokeratin 19-positive cells, which were also tracked in the outer root sheath between bulge and the hair follicle matrix. Remarkably, cytokeratin 19-expressing cells were never detected in the hair follicle infundibulum. Yet, cytokeratin 19-expressing keratinocytes were found in the epidermal stratum basale of normal skin as a subpopulation of cytokeratin 15-positive (not C8/144B-positive) basal keratinocytes. Cytokeratin 19/cytokeratin 15-positive keratinocytes decreased significantly with age. We suggest that cytokeratin 19-expressing cells represent a subpopulation of basal keratinocytes in neonates and young children (up to 1.5 years) that is particularly adapted to the lateral expansion of growing skin. Our data show that cytokeratin 19 in combination with cytokeratin 15 is an important marker to routinely monitor epidermal homeostasis and (at least indirectly) the self-renewing potential of engineered skin.
authors: Tissue Biology Research Unit, Department of Surgery, University Children's Hospital, Zurich, Switzerland.
authors: Luca
authors: L
authors: Pontiggia
authors: Thomas
authors: T
authors: Biedermann
authors: Martin
authors: M
authors: Meuli
authors: Daniel
authors: D
authors: Widmer
authors: Sophie
authors: S
authors: Böttcher-Haberzeth
authors: Clemens
authors: C
authors: Schiestl
authors: Jörg
authors: J
authors: Schneider
authors: Erik
authors: E
authors: Braziulis
authors: Irene
authors: I
authors: Montaño
authors: Claudia
authors: C
authors: Meuli-Simmen
authors: Ernst
authors: E
authors: Reichmann
mesh_terms: D000918:Antibody Specificity; D015415:Biomarkers / Q000378:metabolism*; D002478:Cells, Cultured; D000078404:Epidermal Cells; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D053569:Keratin-15 / Q000276:immunology / Q000378:metabolism; D053539:Keratin-19 / Q000276:immunology / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D053564:Keratins, Hair-Specific / Q000276:immunology / Q000378:metabolism; D009924:Organ Culture Techniques; D011786:Quality Control; D016038:Skin Transplantation / Q000379:methods* / Q000592:standards; D017768:Skin, Artificial / Q000592:standards*; D023822:Tissue Engineering
chemical_list: D015415:Biomarkers; D053569:Keratin-15; D053539:Keratin-19; D053564:Keratins, Hair-Specific