title: Decreased total antioxidant capacity in plasma, but not tissue, in experimental colitis.
abstract: The aim of the present work was to compare colonic mucosa and plasmatic oxidative stress measured concomitantly and with different degrees of injury in rats with colitis induced by trinitrobenzene sulfonic acid. Three groups were studied: control group, colitis group, and colitis exacerbated by diclofenac. Enzymatic markers of colon injury showed enhanced activity in both groups with colitis. The colitis group treated with diclofenac presented higher colonic damage score than the other groups. In both groups with colitis, higher values of tert butyl hydroperoxide-initiated-chemiluminescence and thiobarbituric acid-reactive substances in tissue and decreased total radical-trapping antioxidant potential (TRAP) levels in plasma were found. In conclusion, independently of the degree of colonic mucosa injury and inflammation, oxidative stress in tissue occurs as a consequence of pro-oxidants increase, and is not explained by a reduction of antioxidant defenses. In both conditions, TRAP determination decreases in plasma, but not in tissue.
authors: Department of Pathology, Clinical Analysis and Toxicology, University of Londrina, Londrina, Paraná, Brazil.
authors: José Wander
authors: JW
authors: Breganó
authors: Jane Bandeira
authors: JB
authors: Dichi
authors: Décio Sabbatini
authors: DS
authors: Barbosa
authors: Mirian Zebian
authors: MZ
authors: El Kadri
authors: Tiemi
authors: T
authors: Matsuo
authors: Maria Aparecida
authors: MA
authors: Rodrigues
authors: Rubens
authors: R
authors: Cecchini
authors: Isaias
authors: I
authors: Dichi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000201:enzymology / Q000473:pathology; D016861:Cyclooxygenase Inhibitors; D004008:Diclofenac; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016861:Cyclooxygenase Inhibitors; D004008:Diclofenac; D014302:Trinitrobenzenesulfonic Acid