[Spectral changes of C-phycocyanin with different molar ratios of SPDP].
Pure C-phycocyanin was prepared from Spirulina platensis using one-step anion-exchange chromatography. The C-PC obtained was with an absorption maximum at 620 nm and a fluorescence emission maximum at 640 nm when excited by 580 nm. SPDP is an excellent heterobifunctional crosslinker for thiolating amines. Different molar ratios of SPDP have remarkable influence on the absorption and fluorescence spectra of C-phycocyanin. The absorption maximum and fluorescence emission maximum both decreased and blue-shifted from 640 nm to 630 nm as the molar ratios of SPDP increased. It was found that the molar ratios of SPDP to C-phycocyanin was not more than 100 was appropriate to being conjugated with other biomolecules from the absorption and fluorescence spectra of C-phycocyanin.
Yan
Shi-Gan
SG
State Key Lab of Microbial Technology, Shandong University, Ji'nan 250100, China. yanshigan@yahoo.com.cn
Chen
Xiu-Lan
XL
Zhang
Xi-Ying
XY
Zhou
Bai-Cheng
BC
Zhang
Yu-Zhong
YZ