title: Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
abstract: Abstract Cadherins are synthesized with a signal sequence and a proregion that must be removed for optimal adhesive activity. Mutations that prevent processing of cadherins have been implicated in a number of human diseases; thus understanding their processing is critical. In this study, we produced and characterized a number of monoclonal antibodies against the proregion of the desmosomal cadherin, human desmoglein-2, that will facilitate investigations into the processing of this protein.
authors: University of Nebraska Medical Center, Department of Genetics, Cell Biology and Anatomy, Omaha, Nebraska 68198-7696, USA.
authors: Sarah A
authors: SA
authors: Keim
authors: Keith R
authors: KR
authors: Johnson
authors: Margaret J
authors: MJ
authors: Wheelock
authors: James K
authors: JK
authors: Wahl
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology* / Q000302:isolation & purification; D002462:Cell Membrane / Q000378:metabolism; D051301:Desmoglein 2 / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005260:Female; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D017434:Protein Structure, Tertiary; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; C495314:DSG2 protein, human; D051301:Desmoglein 2