Diversity of endophytic bacteria from Eucalyptus species seeds and colonization of seedlings by Pantoea agglomerans.
The diversity and beneficial characteristics of endophytic microorganisms have been studied in several host plants. However, information regarding naturally occurring seed-associated endophytes and vertical transmission among different life-history stages of hosts is limited. Endophytic bacteria were isolated from seeds and seedlings of 10 Eucalyptus species and two hybrids. The results showed that endophytic bacteria, such as Bacillus, Enterococcus, Paenibacillus and Methylobacterium, are vertically transferred from seeds to seedlings. In addition, the endophytic bacterium Pantoea agglomerans was tagged with the gfp gene, inoculated into seeds and further reisolated from seedlings. These results suggested a novel approach to change the profile of the plants, where the bacterium is a delivery vehicle for desired traits. This is the first report of an endophytic bacterial community residing in Eucalyptus seeds and the transmission of these bacteria from seeds to seedlings. The bacterial species reported in this work have been described as providing benefits to host plants. Therefore, we suggest that endophytic bacteria can be transmitted vertically from seeds to seedlings, assuring the support of the bacterial community in the host plant.
Ferreira
Anderson
A
Departmento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba, SP, Brazil.
Quecine
Maria Carolina
MC
Lacava
Paulo Teixeira
PT
Oda
Shinitiro
S
Azevedo
João Lúcio
JL
Araújo
Welington Luiz
WL
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D044822:Biodiversity; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis; D005052:Eucalyptus / Q000382:microbiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D020636:Pantoea / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D036226:Seedlings / Q000382:microbiology*; D012639:Seeds / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D049452:Green Fluorescent Proteins