title: Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains.
abstract: An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.
authors: Departamento de Microbiología y Parasitología, Universidad de Navarra, c/ Irunlarrea no. 1, 31008 Pamplona, Spain. ilgoni@unav.es
authors: I
authors: I
authors: López-Goñi
authors: D
authors: D
authors: García-Yoldi
authors: C M
authors: CM
authors: Marín
authors: M J
authors: MJ
authors: de Miguel
authors: P M
authors: PM
authors: Muñoz
authors: J M
authors: JM
authors: Blasco
authors: I
authors: I
authors: Jacques
authors: M
authors: M
authors: Grayon
authors: A
authors: A
authors: Cloeckaert
authors: A C
authors: AC
authors: Ferreira
authors: R
authors: R
authors: Cardoso
authors: M I
authors: MI
authors: Corrêa de Sá
authors: K
authors: K
authors: Walravens
authors: D
authors: D
authors: Albert
authors: B
authors: B
authors: Garin-Bastuji
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002002:Brucella / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008322:Mammals; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers