title: Endocrine effects of growth hormone overexpression in transgenic coho salmon.
abstract: Non-transgenic (wild-type) coho salmon (Oncorhynchus kisutch), growth hormone (GH) transgenic salmon (with highly elevated growth rates), and GH transgenic salmon pair fed a non-transgenic ration level (and thus growing at the non-transgenic rate) were examined for plasma hormone concentrations, and liver, muscle, hypothalamus, telencephalon, and pituitary mRNA levels. GH transgenic salmon exhibited increased plasma GH levels, and enhanced liver, muscle and hypothalamic GH mRNA levels. Insulin-like growth factor-I (IGF-I) in plasma, and growth hormone receptor (GHR) and IGF-I mRNA levels in liver and muscle, were higher in fully fed transgenic than non-transgenic fish. GHR mRNA levels in transgenic fish were unaffected by ration-restriction, whereas plasma GH was increased and plasma IGF-I and liver IGF-I mRNA were decreased to wild-type levels. These data reveal that strong nutritional modulation of IGF-I production remains even in the presence of constitutive ectopic GH expression in these transgenic fish. Liver GHR membrane protein levels were not different from controls, whereas, in muscle, GHR levels were elevated approximately 5-fold in transgenic fish. Paracrine stimulation of IGF-I by ectopic GH production in non-pituitary tissues is suggested by increased basal cartilage sulphation observed in the transgenic salmon. Levels of mRNA for growth hormone-releasing hormone (GHRH) and cholecystokinin (CCK) did not differ between groups. Despite its role in appetite stimulation, neuropeptide Y (NPY) mRNA was not found to be elevated in transgenic groups.
authors: Department of Fisheries & Oceans, Centre for Aquaculture & Environmental Research, West Vancouver, BC, Canada.
authors: P A
authors: PA
authors: Raven
authors: M
authors: M
authors: Uh
authors: D
authors: D
authors: Sakhrani
authors: B R
authors: BR
authors: Beckman
authors: K
authors: K
authors: Cooper
authors: J
authors: J
authors: Pinter
authors: E H
authors: EH
authors: Leder
authors: J
authors: J
authors: Silverstein
authors: R H
authors: RH
authors: Devlin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D002766:Cholecystokinin / Q000235:genetics; D013006:Growth Hormone / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D013007:Growth Hormone-Releasing Hormone / Q000235:genetics; D007031:Hypothalamus / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009478:Neuropeptide Y / Q000235:genetics; D017684:Oncorhynchus kisutch / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011986:Receptors, Somatotropin / Q000235:genetics; D013687:Telencephalon / Q000378:metabolism
chemical_list: D009478:Neuropeptide Y; D012333:RNA, Messenger; D011986:Receptors, Somatotropin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D002766:Cholecystokinin; D013007:Growth Hormone-Releasing Hormone