title: The involvement of sortase A in high virulence of STSS-causing Streptococcus suis serotype 2.
abstract: Sortase A (SrtA), originally identified as a transpeptidase in Staphylococcus aureus, plays key roles in full virulence of pathogenic bacteria. In silico genome-wide search suggested a srtA homologue from 05ZYH33, a Chinese human isolate of streptococcal toxic shock syndrome (STSS)-causing Streptococcus suis serotype 2 (S. suis 2, SS2). An isogenic srtA mutant (DeltasrtA) of 05ZYH33 strain was obtained by homologous recombination. Immunofluorescence analysis revealed that two known virulence-associated surface proteins featuring Leu-Pro-X-Thr-Gly motif, muramidase-released protein and surface antigen one, were absent in the DeltasrtA. Piglet infection experiments showed that deletion of srtA attenuated the full virulence of 05ZYH33 strain, and impaired its colonizing potential in specific organs. Furthermore, the DeltasrtA displayed significant reduction in adherence to human cells (Hep-2 and human umbilical vein endothelial cells). Collectively, we concluded that SrtA was involved in the virulence manifestation of STSS-causing SS2.
authors: Department of Pathogen Biology, Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors: Changjun
authors: C
authors: Wang
authors: Ming
authors: M
authors: Li
authors: Youjun
authors: Y
authors: Feng
authors: Feng
authors: F
authors: Zheng
authors: Yaqing
authors: Y
authors: Dong
authors: Xiuzhen
authors: X
authors: Pan
authors: Gong
authors: G
authors: Cheng
authors: Ruiping
authors: R
authors: Dong
authors: Dan
authors: D
authors: Hu
authors: Xiaodan
authors: X
authors: Feng
authors: Junchao
authors: J
authors: Ge
authors: Di
authors: D
authors: Liu
authors: Jing
authors: J
authors: Wang
authors: Min
authors: M
authors: Cao
authors: Fuquan
authors: F
authors: Hu
authors: Jiaqi
authors: J
authors: Tang
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D042783:Endothelial Cells / Q000382:microbiology; D006801:Humans; D011897:Random Allocation; D012772:Shock, Septic / Q000382:microbiology*; D013290:Streptococcal Infections / Q000382:microbiology*; D017011:Streptococcus suis / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D013552:Swine; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases