title: Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis.
abstract: Cellular diversity of the brain is largely attributed to the spatial and temporal heterogeneity of progenitor cells. In mammalian cerebral development, it has been difficult to determine how heterogeneous the neural progenitor cells are, owing to dynamic changes in their nuclear position and gene expression. To address this issue, we systematically analyzed the cDNA profiles of a large number of single progenitor cells at the mid-embryonic stage in mouse. By cluster analysis and in situ hybridization, we have identified a set of genes that distinguishes between the apical and basal progenitors. Despite their relatively homogeneous global gene expression profiles, the apical progenitors exhibit highly variable expression patterns of Notch signaling components, raising the possibility that this causes the heterogeneous division patterns of these cells. Furthermore, we successfully captured the nascent state of basal progenitor cells. These cells are generated shortly after birth from the division of the apical progenitors, and show strong expression of the major Notch ligand delta-like 1, which soon fades away as the cells migrate in the ventricular zone. We also demonstrated that attenuation of Notch signals immediately induces differentiation of apical progenitors into nascent basal progenitors. Thus, a Notch-dependent feedback loop is likely to be in operation to maintain both progenitor populations.
authors: Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN Kobe Institute, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan. akawa@cdb.riken.jp
authors: Ayano
authors: A
authors: Kawaguchi
authors: Tomoko
authors: T
authors: Ikawa
authors: Takeya
authors: T
authors: Kasukawa
authors: Hiroki R
authors: HR
authors: Ueda
authors: Kazuki
authors: K
authors: Kurimoto
authors: Mitinori
authors: M
authors: Saitou
authors: Fumio
authors: F
authors: Matsuzaki
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019070:Cell Lineage / Q000235:genetics; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D009420:Nervous System / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D038081:Organogenesis / Q000502:physiology*; D016548:Prosencephalon / Q000166:cytology; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013234:Stem Cells / Q000145:classification / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D018076:DNA, Complementary; D051880:Receptors, Notch