title: The functional expression and characterisation of a cysteine peptidase from the invasive stage of the neuropathogenic schistosome Trichobilharzia regenti.
abstract: A transcriptional product of a gene encoding cathepsin B-like peptidase in the bird schistosome Trichobilharzia regenti was identified and cloned. The enzyme was named TrCB2 due to its 77% sequence similarity to cathepsin B2 from the important human parasite Schistosoma mansoni. The zymogen was expressed in the methylotropic yeast Pichia pastoris; procathepsin B2 underwent self-processing in yeast media. The peptidolytic activity of the recombinant enzyme was characterised using synthetic fluorogenic peptide substrates at optimal pH 6.0. Functional studies using different specific inhibitors proved the typical cathepsin B-like nature of the enzyme. The S(2) subsite specificity profile of recombinant TrCB2 was obtained. Using monospecific antibodies against the recombinant enzyme, the presence of cathepsin B2 was confirmed in extracts from cercariae (infective stage) and schistosomula (early post-cercarial stage) of T. regenti on Western blots. Also, cross-reactivity was observed between T. regenti and S. mansoni cathepsins B2 in extracts of cercariae, schistosomula or adults. In T. regenti, the antisera localised the enzyme to post-acetabular penetration glands of cercariae implying an important role in the penetration of host skin. The ability of recombinant TrCB2 to degrade skin, serum and nervous tissue proteins was evident. Elastinolytic activity suggests that the enzyme might functionally substitute the histolytic role of the serine class elastase known from S. mansoni and Schistosoma haematobium but not found in Schistosoma japonicum or in bird schistosomes.
authors: Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicná 7, 12844 Prague 2, Czech Republic.
authors: Katerina
authors: K
authors: Dolecková
authors: Martin
authors: M
authors: Kasný
authors: Libor
authors: L
authors: Mikes
authors: Jared
authors: J
authors: Cartwright
authors: Petr
authors: P
authors: Jedelský
authors: Eric L
authors: EL
authors: Schneider
authors: Jan
authors: J
authors: Dvorák
authors: Adrian P
authors: AP
authors: Mountford
authors: Charles S
authors: CS
authors: Craik
authors: Petr
authors: P
authors: Horák
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002401:Cathepsin B / Q000096:biosynthesis; D002417:Cattle; D003545:Cysteine / Q000096:biosynthesis*; D057056:Cysteine Proteases / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology*; D004372:Ducks; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010843:Pichia; D011817:Rabbits; D012547:Schistosoma / Q000201:enzymology*; D012550:Schistosoma mansoni / Q000201:enzymology; D012908:Snails; D013379:Substrate Specificity; D014422:Turkeys
chemical_list: D057056:Cysteine Proteases; D002401:Cathepsin B; D003545:Cysteine