[Genetic diversity evaluation of ex-situ conservation population of Phellodendron amurense detected by AFLP markers].
OBJECTIVE
To evaluate the genetic diversity from ex-situ conservation population of Phellodendron amurense.


METHODS
Genetic diversity in 67 individuals from ex-situ conservation population of P. amurense were assessed using AFLP technique by eight fluorescent labeled primer groups.


RESULTS
The eight primer combinations generated a total of 1,574 bands, of which 1,354 were polymorphic. As for P. amurense at species level, the percentage of polymorphic loci (PPL) was 86.02, the effective number of alleles per locus (Ne) was 1.4275 ,the Nei's gene diversity (H) was 0.2312, and the Shannon's information index (I) was 0.3973. At the population level, the estimates PPL=41.55, H=0.1400, Ne=0.2376 and I=0.2113.


CONCLUSIONS
The effect of present ex-situ conservation was preliminarily assessed.
Yan
Zhi-Feng
ZF
Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100094, China.
Zhang
Ben-Gang
BG
Zhang
Zhao
Z
D054458:Amplified Fragment Length Polymorphism Analysis; D003247:Conservation of Natural Resources; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D032102:Phellodendron / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic
D017931:DNA Primers