[Application of single drop microextraction in the determination of phthalate esters and parabens in drugs by gas chromatography-mass spectrometry].
Single drop microextraction (SDME) was used for the determination of phthalate esters and parabens in drugs by gas chromatography-ion trap mass spectrometry (GC-IT/MS). The effects of the nature of extraction solvents, microdrop volume, the depth of microdrop in sample solution, extraction time and stirring rate on the extraction efficiency were investigated separately. The optimal SDME conditions, 1.5 microL of toluene, 0.8 cm of the depth of microdrop, 1 000 r/min of stirring rate and 20 mm of extraction time, were obtained and used for the analysis of methylparaben (MP), ethylparaben (EP), propylparaben (PP), iso-propylparaben (IPP), butylparaben (BP), dimethyl phthalate (DMP), diethyl phthalate (DEP) and dibutyl phthalate (DBP) in drugs. The results showed that the working curves for 8 phthalate esters and parabens were linear in the range of 0.032 - 80 mg/L by GC-MS on selective ion storage mode. The limits of detection (LOD) were between 0.6 microg/L and 1.28 mg/L, the overall recoveries were 95.85% - 148.85% with the relative standard deviations of 3.9% - 14.9%.
Qi
Aiming
A
Hunan Normal University, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Changsha 410081, China. qam3776@163.com
Li
Meigui
M
Mao
Liqiu
L
D005591:Chemical Fractionation / Q000379:methods*; D004952:Esters / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D057230:Limit of Detection; D016014:Linear Models; D010226:Parabens / Q000032:analysis* / Q000302:isolation & purification*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D010795:Phthalic Acids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
D004952:Esters; D010226:Parabens; D004364:Pharmaceutical Preparations; D010795:Phthalic Acids; D012997:Solvents; C032279:phthalic acid