title: Virulence in murine model shows the existence of two distinct populations of Brazilian Vaccinia virus strains.
abstract: Brazilian Vaccinia virus had been isolated from sentinel mice, rodents and recently from humans, cows and calves during outbreaks on dairy farms in several rural areas in Brazil, leading to high economic and social impact. Some phylogenetic studies have demonstrated the existence of two different populations of Brazilian Vaccinia virus strains circulating in nature, but little is known about their biological characteristics. Therefore, our goal was to study the virulence pattern of seven Brazilian Vaccinia virus strains. Infected BALB/c mice were monitored for morbidity, mortality and viral replication in organs as trachea, lungs, heart, kidneys, liver, brain and spleen. Based on the virulence potential, the Brazilian Vaccinia virus strains were grouped into two groups. One group contained GP1V, VBH, SAV and BAV which caused disease and death in infected mice and the second one included ARAV, GP2V and PSTV which did not cause any clinical signals or death in infected BALB/c mice. The subdivision of Brazilian Vaccinia virus strains into two groups is in agreement with previous genetic studies. Those data reinforce the existence of different populations circulating in Brazil regarding the genetic and virulence characteristics.
authors: Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais.Avenida Antônio Carlos, Belo Horizonte, Brazil.
authors: Jaqueline Maria Siqueira
authors: JM
authors: Ferreira
authors: Betânia Paiva
authors: BP
authors: Drumond
authors: Maria Isabel Maldonado Coelho
authors: MI
authors: Guedes
authors: Marcelo Antônio
authors: MA
authors: Pascoal-Xavier
authors: Camila Megale
authors: CM
authors: Almeida-Leite
authors: Rosa Maria Esteves
authors: RM
authors: Arantes
authors: Bruno Eduardo Fernandes
authors: BE
authors: Mota
authors: Jônatas Santos
authors: JS
authors: Abrahão
authors: Pedro Augusto
authors: PA
authors: Alves
authors: Fernando Meireles
authors: FM
authors: Oliveira
authors: Paulo César Peregrino
authors: PC
authors: Ferreira
authors: Cláudio Antônio
authors: CA
authors: Bonjardim
authors: Zélia Inês Portela
authors: ZI
authors: Lobato
authors: Erna Geessien
authors: EG
authors: Kroon
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology; D004196:Disease Outbreaks; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D014615:Vaccinia / Q000453:epidemiology / Q000401:mortality / Q000662:veterinary; D014616:Vaccinia virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics