Chemoprotective effects of a protein from the red algae Porphyra yezoensis on acetaminophen-induced liver injury in rats.
Seaweeds contribute to the maintenance of health through their nutritional and medicinal properties. The effects of PYP, a 14 kDa protein isolated from a hot-water extract of the marine alga Porphyra yezoensis, on AAP-induced liver injury in rats was evaluated. AAP induced acute liver injury and AAP-induced hepatotoxicity is the leading cause of liver failure. In this study, male Sprague-Dawley rats were assigned to one of three treatment groups: control, AAP, or AAP + PYP. Compared with the control group, liver tissue from the AAP group showed increased levels of caspase-3 activity and DNA fragmentation, decreased levels of GSH and increased serum GOT/GPT levels. In contrast, treatment with AAP + PYP produced levels of caspase-3 activity, DNA fragmentation, GSH and GOT/GPT that matched the values seen in the control group. It is concluded that PYP may prevent AAP-induced liver injury.
Hwang
Hye-Jung
HJ
Faculty of Food Science and Biotechnology, Pukyong National University, Busan 608-737, Korea.
Kwon
Mi-Jin
MJ
Kim
In-Hye
IH
Nam
Taek-Jeong
TJ
D000082:Acetaminophen / Q000633:toxicity*; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology / Q000473:pathology; D053938:DNA Fragmentation / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000378:metabolism; D008297:Male; D010940:Plant Proteins / Q000627:therapeutic use*; D044664:Porphyra / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D010940:Plant Proteins; D000082:Acetaminophen; D053148:Caspase 3; D005978:Glutathione