Differentially expressed protein profile of human dental pulp cells in the early process of odontoblast-like differentiation in vitro.
Dental pulp cells (DPCs) are capable of differentiating into odontoblasts that secrete reparative dentin after pulp injury. The molecular mechanisms governing reparative dentinogenesis are yet to be fully understood. Here we investigated the differential protein profile of human DPCs undergoing odontogenic induction for 7 days. Using two-dimensional differential gel electrophoresis coupled with matrix-assisted laser adsorption ionization time of flight mass spectrometry, 23 protein spots related to the early odontogenic differentiation were identified. These proteins included cytoskeleton proteins, nuclear proteins, cell membrane-bound molecules, proteins involved in matrix synthesis, and metabolic enzymes. The expression of four identified proteins, which were heteronuclear ribonuclear proteins C, annexin VI, collagen type VI, and matrilin-2, was confirmed by Western blot and real-time real-time polymerase chain reaction analyses. This study generated a proteome reference map during odontoblast-like differentiation of human DPCs, which will be valuable to better understand the underlying molecular mechanisms in odontoblast-like differentiation.
Wei
Xi
X
Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China.
Wu
Liping
L
Ling
Junqi
J
Liu
Lu
L
Liu
Shaojun
S
Liu
Wei
W
Li
Mingtao
M
Xiao
Yin
Y
D000328:Adult; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000096:biosynthesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000096:biosynthesis; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism*; D003809:Dentin, Secondary / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D020869:Gene Expression Profiling; D006801:Humans; D020935:MAP Kinase Signaling System; D009687:Nuclear Proteins / Q000096:biosynthesis; D009804:Odontoblasts / Q000378:metabolism*; D054464:Peroxiredoxins / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002135:Calcium-Binding Proteins; D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins; D009687:Nuclear Proteins; D020543:Proteome; D054464:Peroxiredoxins