A study of the Candida albicans cell wall proteome.
Considering the importance of proteins in the structure and function of the cell wall of Candida albicans, we analyzed the cell wall subproteome of this important human pathogen by LC coupled to MS (LC-MS) using different protein extraction procedures. The analyzed samples included material extracted by hydrogen fluoride-pyridine (HF-pyridine), and whole SDS-extracted cell walls. The use of this latter innovative procedure gave similar data as compared to the analysis of HF-pyridine extracted proteins. A total of 21 cell wall proteins predicted to contain a signal peptide were identified, together with a high content of potentially glycosylated Ser/Thr residues, and the presence of a GPI motif in 19 of them. We also identified 66 "atypical" cell wall proteins that lack the above-mentioned characteristics. After tryptic removal of the most accessible proteins in the cell wall, several of the same expected GPI proteins and the most commonly found "atypical" wall proteins were identified. This result suggests that proteins are located not only at the cell wall surface, but are embedded within the cell wall itself. These results, which include new identified cell wall proteins, and comparison of proteins in blastospore and mycelial walls, will help to elucidate the C. albicans cell wall architecture.
Castillo
Luis
L
Departament de Microbiología i Ecología, Facultat de Farmacia, Universitat de València, Burjassot, Spain.
Calvo
Enrique
E
Martínez
Ana I
AI
Ruiz-Herrera
José
J
Valentín
Eulogio
E
Lopez
Juan A
JA
Sentandreu
Rafael
R
D002176:Candida albicans / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D006858:Hydrofluoric Acid; D020543:Proteome / Q000378:metabolism*; D011725:Pyridines; D012967:Sodium Dodecyl Sulfate; D012997:Solvents; D053719:Tandem Mass Spectrometry
D005656:Fungal Proteins; D020543:Proteome; D011725:Pyridines; D012997:Solvents; D012967:Sodium Dodecyl Sulfate; C023666:pyridine; D006858:Hydrofluoric Acid