A high-temporal resolution technology for dynamic proteomic analysis based on 35S labeling.
As more and more research efforts have been attracted to dynamic or differential proteomics, a method with high temporal resolution and high throughput is required. In present study, a (35)S in vivo Labeling Analysis for Dynamic Proteomics (SiLAD) was designed and tested by analyzing the dynamic proteome changes in the highly synchronized A549 cells, as well as in the rat liver 2/3 partial hepatectomy surgery. The results validated that SiLAD technique, in combination with 2-Dimensional Electrophoresis, provided a highly sensitivity method to illustrate the non-disturbed endogenous proteins dynamic changes with a good temporal resolution and high signal/noise ratio. A significant number of differential proteins can be discovered or re-categorized by this technique. Another unique feature of SiLAD is its capability of quantifying the rate of protein expression, which reflects the cellular physiological turn points more effectively. Finally, the prescribed SiLAD proteome snapshot pattern could be potentially used as an exclusive symbol for characterizing each stage in well regulated biological processes.
Zhang
Zhao
Z
Universities' Confederated Institute of Proteomics, Key laboratory for Cell Proliferation and Regulation Biology Ministry of Education, Beijing Normal University, Beijing, People's Republic of China.
Chen
Jian
J
Guo
Fuzheng
F
He
Liren
L
Wu
Yizhou
Y
Zeng
Changqing
C
Xiao
Xueyuan
X
He
Dacheng
D
D002453:Cell Cycle; D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D008175:Lung Neoplasms; D009363:Neoplasm Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D016196:S Phase; D012984:Software; D013462:Sulfur Radioisotopes
D009363:Neoplasm Proteins; D011506:Proteins; D020543:Proteome; D013462:Sulfur Radioisotopes