Investigation of carbon nanofibers as support for bioactive substances.
In this paper we have studied the adsorption properties of various bio-active systems onto the surface of carbon nanofibers (CNF) synthesized by chemical vapor deposition (CVD). Amino acids (alanine, aspartic acid, glutamic acid) and glucose oxidase (GOx) were adsorbed on CNF and the results were compared with those obtained when activated carbon (AC) was used as support. CNF and AC properties (hydrophilic or hydrophobic properties) were characterized by the pH value, the concentration of acidic/basic sites and by naphthalene adsorption. CNF with immobilized GOx was additionally investigated as a highly sensitive glucose biosensor. An amperometric method was used in an original manner to detect the changes in the specific activity of GOx, immobilized longer time on CNF. The method demonstrates that not the whole enzyme adsorbed onto CNF can catalyze the oxidation of glucose from the solution.
Olenic
Liliana
L
National Institute for R&D of Isotopic and Molecular Technologies, Cluj-Napoca, Romania. olenic@itim-cj.ro
Mihailescu
Gheorghe
G
Pruneanu
Stela
S
Lupu
Dan
D
Biris
Alexandru R
AR
Margineanu
Petre
P
Garabagiu
Sorina
S
Biris
Alexandru S
AS
D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure
D000596:Amino Acids; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D002244:Carbon; D005949:Glucose Oxidase