title: Mutagenic and recombinagenic responses to defective DNA polymerase delta are facilitated by the Rev1 protein in pol3-t mutants of Saccharomyces cerevisiae.
abstract: Defective DNA replication can result in substantial increases in the level of genome instability. In the yeast Saccharomyces cerevisiae, the pol3-t allele confers a defect in the catalytic subunit of replicative DNA polymerase delta that results in increased rates of mutagenesis, recombination, and chromosome loss, perhaps by increasing the rate of replicative polymerase failure. The translesion polymerases Pol eta, Pol zeta, and Rev1 are part of a suite of factors in yeast that can act at sites of replicative polymerase failure. While mutants defective in the translesion polymerases alone displayed few defects, loss of Rev1 was found to suppress the increased rates of spontaneous mutation, recombination, and chromosome loss observed in pol3-t mutants. These results suggest that Rev1 may be involved in facilitating mutagenic and recombinagenic responses to the failure of Pol delta. Genome stability, therefore, may reflect a dynamic relationship between primary and auxiliary DNA polymerases.
authors: Division of Molecular Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, California 91010-3000, USA.
authors: Erica
authors: E
authors: Mito
authors: Janet V
authors: JV
authors: Mokhnatkin
authors: Molly C
authors: MC
authors: Steele
authors: Victoria L
authors: VL
authors: Buettner
authors: Steve S
authors: SS
authors: Sommer
authors: Glenn M
authors: GM
authors: Manthey
authors: Adam M
authors: AM
authors: Bailis
mesh_terms: D000483:Alleles; D004258:DNA Polymerase III / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D000099270:Y-Family DNA Polymerases
chemical_list: D004258:DNA Polymerase III; D009713:Nucleotidyltransferases; D029701:Saccharomyces cerevisiae Proteins; C491232:POL3 protein, S cerevisiae; C100951:REV1 protein, S cerevisiae; D000099270:Y-Family DNA Polymerases