title: Intrathecal veratridine administration increases minimum alveolar concentration in rats.
abstract: BACKGROUND
  Results from several studies point to sodium channels as potential mediators of the immobility produced by inhaled anesthetics. We hypothesized that the intrathecal administration of veratridine, a drug that enhances the activity or effect of sodium channels, should increase MAC.
  
  
  METHODS
  We measured the change in isoflurane MAC caused by intrathecal infusion of various concentrations of veratridine into the lumbothoracic subarachnoid space of rats. We compared these result with those obtained from intracerebroventricular infusion.
  
  
  RESULTS
  As predicted, intrathecal infusion of veratridine increased MAC. The greatest infused concentration (25 microM) also produced neuronal injury in the hindlimbs of two rats and decreased the peak effect on MAC. A concentration of 1.6 microM produced the largest (21%) increase in MAC. Intraventricular infusion of 1.6 and 6.4 microM veratridine did not alter MAC. Rats given 25 microM died.
  
  
  CONCLUSIONS
  Intrathecal administration of veratradine increases MAC of isoflurane, a finding consistent with a role for sodium channels as potential mediators of the immobility produced by inhaled anesthetics.
authors: Department of Anesthesiology, Fuwai Hospital and Cardiovascular Institute, Beijing, China.
authors: Yi
authors: Y
authors: Zhang
authors: Manohar
authors: M
authors: Sharma
authors: Edmond I
authors: EI
authors: Eger
authors: Michael J
authors: MJ
authors: Laster
authors: Hugh C
authors: HC
authors: Hemmings
authors: R Adron
authors: RA
authors: Harris
mesh_terms: D000758:Anesthesia / Q000379:methods; D000777:Anesthetics / Q000008:administration & dosage; D000818:Animals; D004305:Dose-Response Relationship, Drug; D007103:Immobilization; D020128:Inhibitory Concentration 50; D007278:Injections, Spinal / Q000379:methods*; D007530:Isoflurane / Q000494:pharmacology; D008297:Male; D011650:Pulmonary Alveoli / Q000187:drug effects*; D051381:Rats; D020318:Rats, Long-Evans; D015222:Sodium Channels / Q000737:chemistry; D013346:Subarachnoid Space / Q000378:metabolism; D014701:Veratridine / Q000008:administration & dosage*
chemical_list: D000777:Anesthetics; D015222:Sodium Channels; D014701:Veratridine; D007530:Isoflurane