title: Analysis of rhamnolipid biosurfactants produced through submerged fermentation using orange fruit peelings as sole carbon source.
abstract: The fermentative production of rhamnolipid biosurfactant from Pseudomonas aeruginosa MTCC 2297 was carried out by submerged fermentation using various cost-effective waste materials such as orange peelings, carrot peel waste, lime peelings, coconut oil cake, and banana waste. The orange peel was found to be the best substrate generating 9.18 g/l of rhamnolipid biosurfactant with a surface tension reduction up to 31.3 mN/m. The production was growth independent, and optimum conditions were standardized. The emulsifying activity was highest against kerosene (73.3%). Rhamnolipid components were purified and separated by ethyl acetate extraction, preparative silica gel column chromatography, high-performance liquid chromatography and thin-layer chromatography. The major rhamnolipid components were characterized, by fast atom bombardment mass spectrometry, as a mixture of dirhamnolipids and monorhamnolipids.
authors: School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills P.O, Athirampuzha, Kottayam District, Kerala 686 560, India.
authors: Seba
authors: S
authors: George
authors: K
authors: K
authors: Jayachandran
mesh_terms: D002244:Carbon / Q000378:metabolism*; D032084:Citrus sinensis; D003470:Culture Media; D005285:Fermentation; D006017:Glycolipids / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D013500:Surface Tension; D013501:Surface-Active Agents / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003470:Culture Media; D006017:Glycolipids; D013501:Surface-Active Agents; C418382:rhamnolipid; D002244:Carbon