title: CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.
abstract: NK cell markers and receptors have been discovered in many mammalian species, such as humans, mice, rats, pigs, and cows. However, there is still a lack of information concerning NK cell markers or receptors in canines. We have discovered that canine CD5-low density (CD5lo) cells in PBL are closely associated with NK cell characteristics. CD5lo cells comprised 14.9 +/- 6.68% of the total PBL. A high proportion of the CD5lo cell population expressed CD3 (96.6%), CD8alpha (77.7%), CD8beta (53%), alpha/beta TCR (83%), and CD11/18 (80%), but the expression of gamma/delta TCR (6.5%), CD4 (10.6%), and CD21 (2.4%) was low. CD5lo cells were larger than CD5-high density (CD5hi) cells. Light and electron microscopy revealed numerous large cytoplasmic granules in CD5lo cells, especially after IL-2 stimulation, which was in contrast to CD5hi, in which intracytoplasmic granules were not frequently seen. After IL-2 stimulation, CD5lo cells had significantly stronger NK cytotoxicity than CD5hi cells. CD5lo cells had much higher mRNA levels for NKG2D, CD16, CD94, CD160, perforin, and granzyme than CD5hi. Following IL-2 stimulation, CD5lo cells had significantly higher mRNA levels of NKp30, NKp44, CD16, and CD94 than CD5hi cells. In addition, IL-2-stimulated, CD5lo-depleted PBL showed a loss of NK cytotoxicity. CD5lo cells also showed significantly lower antigen-specific cytotoxic T cell activity as compared with CD5hi cells. Taken together, the CD5lo subset in canine PBL is closely related to canine NK cells, and CD5lo can be used as a phenotypic marker for an IL-2-dependent canine NK cell enrichment.
authors: Animal Cancer Research Center, Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, 1 Roosevelt Road, Section 4, Taipei 106, Taiwan.
authors: Yi-Chun
authors: YC
authors: Huang
authors: Shao-Wen
authors: SW
authors: Hung
authors: Tong-Rong
authors: TR
authors: Jan
authors: Kuang-Wen
authors: KW
authors: Liao
authors: Chiung-Hsiang
authors: CH
authors: Cheng
authors: Yu-Shan
authors: YS
authors: Wang
authors: Rea-Min
authors: RM
authors: Chu
mesh_terms: D000818:Animals; D018956:CD5 Antigens / Q000378:metabolism*; D003601:Cytotoxicity Tests, Immunologic; D004285:Dogs; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D007371:Interferon-gamma / Q000378:metabolism; D019409:Interleukin-15 / Q000378:metabolism; D020382:Interleukin-18 / Q000378:metabolism; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007962:Leukocytes / Q000378:metabolism; D016131:Lymphocyte Subsets; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D018956:CD5 Antigens; D019409:Interleukin-15; D020382:Interleukin-18; D012333:RNA, Messenger; D007371:Interferon-gamma