title: Designed glycopeptides with different beta-turn types as synthetic probes for the detection of autoantibodies as biomarkers of multiple sclerosis.
abstract: Circulating autoantibodies have been recognized as disease biomarkers of autoimmune diseases. We have previously disclosed a synthetic glycopeptide that is able to detect specific autoantibodies in sera of patients who are affected by multiple sclerosis (MS). This glycopeptide is characterized by a type I' beta-turn around the minimal epitope Asn(Glc) that allows an efficient exposure of this moiety to antibody interactions in the context of a solid-phase immunoenzymatic assay. With the aim of optimizing the glycopeptide-antibody interactions, we analyze a series of new glycopeptides based on different turn structures. Our results confirm the role of conformation in the recognition and binding of synthetic antigenic probes to MS autoantibodies. Glycopeptide 2, which is characterized by a type I beta-turn around the minimal epitope Asn(Glc), shows the highest antibody affinity (IC50 = 11.8 nM), and thus it appears to be a promising tool for the detection of specific autoantibodies as MS biomarker in patients' sera.
authors: Department of Pharmaceutical and Toxicological Chemistry, University of Naples Federico II, via D. Montesano 49, I-80131 Naples, Italy.
authors: Alfonso
authors: A
authors: Carotenuto
authors: Maria Claudia
authors: MC
authors: Alcaro
authors: Maria Rosaria
authors: MR
authors: Saviello
authors: Elisa
authors: E
authors: Peroni
authors: Francesca
authors: F
authors: Nuti
authors: Anna Maria
authors: AM
authors: Papini
authors: Ettore
authors: E
authors: Novellino
authors: Paolo
authors: P
authors: Rovero
mesh_terms: D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D015195:Drug Design; D006020:Glycopeptides / Q000138:chemical synthesis / Q000276:immunology; D006801:Humans; D015335:Molecular Probes / Q000138:chemical synthesis*; D009103:Multiple Sclerosis / Q000175:diagnosis*; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D006020:Glycopeptides; D015335:Molecular Probes