Virulence in murine model shows the existence of two distinct populations of Brazilian Vaccinia virus strains.
Brazilian Vaccinia virus had been isolated from sentinel mice, rodents and recently from humans, cows and calves during outbreaks on dairy farms in several rural areas in Brazil, leading to high economic and social impact. Some phylogenetic studies have demonstrated the existence of two different populations of Brazilian Vaccinia virus strains circulating in nature, but little is known about their biological characteristics. Therefore, our goal was to study the virulence pattern of seven Brazilian Vaccinia virus strains. Infected BALB/c mice were monitored for morbidity, mortality and viral replication in organs as trachea, lungs, heart, kidneys, liver, brain and spleen. Based on the virulence potential, the Brazilian Vaccinia virus strains were grouped into two groups. One group contained GP1V, VBH, SAV and BAV which caused disease and death in infected mice and the second one included ARAV, GP2V and PSTV which did not cause any clinical signals or death in infected BALB/c mice. The subdivision of Brazilian Vaccinia virus strains into two groups is in agreement with previous genetic studies. Those data reinforce the existence of different populations circulating in Brazil regarding the genetic and virulence characteristics.
Ferreira
Jaqueline Maria Siqueira
JM
Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais.Avenida Antônio Carlos, Belo Horizonte, Brazil.
Drumond
Betânia Paiva
BP
Guedes
Maria Isabel Maldonado Coelho
MI
Pascoal-Xavier
Marcelo Antônio
MA
Almeida-Leite
Camila Megale
CM
Arantes
Rosa Maria Esteves
RM
Mota
Bruno Eduardo Fernandes
BE
Abrahão
Jônatas Santos
JS
Alves
Pedro Augusto
PA
Oliveira
Fernando Meireles
FM
Ferreira
Paulo César Peregrino
PC
Bonjardim
Cláudio Antônio
CA
Lobato
Zélia Inês Portela
ZI
Kroon
Erna Geessien
EG
D000818:Animals; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology; D004196:Disease Outbreaks; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D014615:Vaccinia / Q000453:epidemiology / Q000401:mortality / Q000662:veterinary; D014616:Vaccinia virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics