title: Heat shock causes a decrease in polysomes and the appearance of stress granules in trypanosomes independently of eIF2(alpha) phosphorylation at Thr169.
abstract: In trypanosomes there is an almost total reliance on post-transcriptional mechanisms to alter gene expression; here, heat shock was used to investigate the response to an environmental signal. Heat shock rapidly and reversibly induced a decrease in polysome abundance, and the consequent changes in mRNA metabolism were studied. Both heat shock and polysome dissociation were necessary for (1) a reduction in mRNA levels that was more rapid than normal turnover, (2) an increased number of P-body-like granules that contained DHH1, SCD6 and XRNA, (3) the formation of stress granules that remained largely separate from the P-body-like granules and localise to the periphery of the cell and, (4) an increase in the size of a novel focus located at the posterior pole of the cell that contain XRNA, but neither DHH1 nor SCD6. The response differed from mammalian cells in that neither the decrease in polysomes nor stress-granule formation required phosphorylation of eIF2alpha at the position homologous to that of serine 51 in mammalian eIF2alpha and in the occurrence of a novel XRNA-focus.
authors: Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK.
authors: Susanne
authors: S
authors: Kramer
authors: Rafael
authors: R
authors: Queiroz
authors: Louise
authors: L
authors: Ellis
authors: Helena
authors: H
authors: Webb
authors: Jörg D
authors: JD
authors: Hoheisel
authors: Christine
authors: C
authors: Clayton
authors: Mark
authors: M
authors: Carrington
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism*; D004249:DNA Damage; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D018869:Heat-Shock Response / Q000502:physiology*; D046228:Microarray Analysis; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013912:Threonine / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000378:metabolism
chemical_list: D015852:Eukaryotic Initiation Factor-2; D015800:Protozoan Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D013912:Threonine