title: Bacterial DNA sample preparation from whole blood using surface-modified Si pillar arrays.
abstract: A novel bacterial DNA sample preparation device for molecular diagnostics has been developed. On the basis of optimized conditions for bacterial adhesion, surface-modified silicon pillar arrays for bacterial cell capture were fabricated, and their ability to capture bacterial cells was demonstrated. The capture efficiency for bacterial cells such as Escherichia coli, Staphylococcus epidermidis, and Streptococcus mutans in buffer solution was over 75% with a flow rate of 400 microL/min. Moreover, the proposed method captured E. coli cells present in 50% whole blood effectively. The captured cells from whole blood were then in- situ lyzed on the surface of the microchip, and the eluted DNA was successfully amplified by qPCR. These results demonstrate that the full process of pathogen capture to DNA isolation from whole blood could be automated in a single microchip.
authors: Bio & Health Lab., Samsung Advanced Institute of Technology (SAIT), Mt. 14-1, Nongseo-Dong, Giheung-Gu, Yongin-Si, Gyeonggi-Do, South Korea, 449-712.
authors: Kyu-Youn
authors: KY
authors: Hwang
authors: Hee-Kyun
authors: HK
authors: Lim
authors: Seong-Young
authors: SY
authors: Jung
authors: Kak
authors: K
authors: Namkoong
authors: Joon-Ho
authors: JH
authors: Kim
authors: Nam
authors: N
authors: Huh
authors: Christopher
authors: C
authors: Ko
authors: Jae-Chan
authors: JC
authors: Park
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D001419:Bacteria / Q000166:cytology / Q000378:metabolism; D002448:Cell Adhesion; D004269:DNA, Bacterial / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004269:DNA, Bacterial; D012825:Silicon