title: Insulin and insulin mutants stimulate glucose uptake in rat adipocytes.
abstract: A simple method to determine the in vitro biological activity of insulin by measuring glucose uptake in the rat adipocytes is presented here. In the presence of insulin, the glucose uptake is 5-6 times more than the basal control. And the uptake of D-[3-(3)H]-glucose is linear as the logarithm of insulin concentration from 0.2 microg/L to 1.0 microg/L. Glucose and 3-O-methyl-glucose inhibit D-[3-(3)H]-glucose uptake into adipocytes. By this method, the in vitro biological activity of [B(2)-Lys]-insulin and [B(3)-Lys]-insulin was measured to be 61.6% and 154% respectively, relative to that of insulin.
authors: Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China.
authors: S
authors: S
authors: Yao
authors: X
authors: X
authors: Zhang
authors: Y
authors: Y
authors: Xu
authors: X
authors: X
authors: Zhang
authors: S
authors: S
authors: Zhu