Expression and functional characterisation of the putative SARS coronavirus non-structural proteins X1-X5.
1. We produced mammalian expression vectors encoding the SARS coronavirus (SARS-CoV) accessory proteins with or without the fluorescence protein tag and cell lines with stable expression of these proteins. 2. The cellular localisation and function of the SARS-CoV accessory proteins was determined. 3. SARS 6 and SARS 8b proteins are localised to the endoplasmic reticulum and nucleus/cytoplasm, respectively, and both proteins stimulate host cell DNA synthesis.
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Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. yuanyuanho@cuhk.edu.hk
D000704:Analysis of Variance; D000818:Animals; D016022:Case-Control Studies; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral / Q000235:genetics; D018613:Microscopy, Confocal; D016133:Polymerase Chain Reaction; D011336:Probability; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000502:physiology; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000235:genetics* / Q000503:physiopathology; D014709:Vero Cells / Q000502:physiology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism
D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; C087633:nonstructural protein, coronavirus