[Regulatory effect of resveratrol on JAK1/STAT3 signal transduction pathway in leukemia].
The aim of this study was to explore the molecule mechanism of resveratrol antileukaemia. The mouse lymphocytic leukemia L1210 cells were cultured and the expressions of pJAK1 and pSTAT3 protein in L1210 cells were detected by immunohistochemistry and immunoprecipitation in vitro. The mouse model with L1210 leukemia ascites carcinoma was established and activities of singal transduction pathway molecules pJAK1 and pSTAT3 were measured by Western blot and immunohistochemistry assay in vitro. The results indicated that resveratrol could significantly inhibit the JAK1/STAT3 signal transduction pathway, down-regulate expressions of pJAK1 and pSTAT3 and reduce the phosphorylation of JAK1 and STAT3 in a dose-and time-dependent manner. It is concluded that the resveratrol can regulate signal transduction pathway and reduce the activation of JAK1/STAT3 tyrosine phosphorylation significantly, and therefore resveratrol shows chemotherapeutic potential to leukaemia.
Li
Tan
T
Department of Immunology, Medical College of the Chinese Armed Police Force, Tianjin 300162, China. litan2002@eyou.com
Wang
Wei
W
Li
Tong
T
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D015536:Down-Regulation; D053613:Janus Kinase 1 / Q000235:genetics* / Q000378:metabolism; D007939:Leukemia L1210 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D000077185:Resveratrol; D050796:STAT3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D013267:Stilbenes; C507905:Jak1 protein, mouse; D053613:Janus Kinase 1; D000077185:Resveratrol