title: Toward a full characterization of native starch: separation and detection by size-exclusion chromatography.
abstract: The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 degrees C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer.
authors: University of Queensland, CNAFS/SLCAFS, Hartley Teakle Building 83, Brisbane , QLD 4072, Australia.
authors: Ngoc-Ly
authors: NL
authors: Hoang
authors: Antonin
authors: A
authors: Landolfi
authors: Anastasiya
authors: A
authors: Kravchuk
authors: Etienne
authors: E
authors: Girard
authors: Jonathan
authors: J
authors: Peate
authors: Javier M
authors: JM
authors: Hernandez
authors: Marianne
authors: M
authors: Gaborieau
authors: Olena
authors: O
authors: Kravchuk
authors: Robert G
authors: RG
authors: Gilbert
authors: Yohann
authors: Y
authors: Guillaneuf
authors: Patrice
authors: P
authors: Castignolles
mesh_terms: D019840:2-Propanol / Q000737:chemistry; D000081:Acetamides; D001965:Bromides; D002850:Chromatography, Gel / Q000379:methods*; D004121:Dimethyl Sulfoxide; D018020:Lithium Compounds; D011142:Polyvinyl Alcohol / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013213:Starch / Q000031:analogs & derivatives / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000081:Acetamides; D001965:Bromides; D018020:Lithium Compounds; C040949:lithium bromide; D011142:Polyvinyl Alcohol; D013213:Starch; C013959:dimethylacetamide; D019840:2-Propanol; D004121:Dimethyl Sulfoxide