Microsatellite DNA variation of the gametophyte clones isolated from introduced Laminaria japonica (Phaeophyta) and L. longissima of China and varieties derived from them.
The variation of 90 Laminaria gametophyte clones representing the introduced Laminaria japonica (Group 1) and Laminaria longissima (Group 2), the varieties of L. japonica (Group 3) and the varieties derived from interspecific hybrids (Group 4) was determined with 18 microsatellite markers. The allelic diversity and Nei's gene diversity of Group 1 were significantly higher than those of Group 2 (2.9 vs. 1.8 and 0.414 vs. 0.161, respectively), demonstrating that the variation of the introduced L. japonica is richer than that of L. longissima. Both allelic diversity and Nei's gene diversity of Group 3 were lower than those of Group 1, indicating that only a portion of variation of L. japonica was incorporated into the varieties of L. japonica. Significant genetic differentiation was detected between four groups and between female (Population 1) and male (Population 2) gametophyte clones in each group. The variation among groups accounted for 39.95%, while that among populations accounted for 21.65% of the total. The genetic distance between Group 1 and Group 4 was obviously longer than that between Group 2 and Group 4 (0.686 vs. 0.291), indicating that maternal gametophyte clone contributed more variation to the hybrids than the paternal gametophyte clone did.
Li
Bing-Jun
BJ
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Shi
Yuan-Yuan
YY
Yang
Guan-Pin
GP
Che
Shi
S
Xiao-Jie
Li
L
Yi-Zhou
Cong
C
D000483:Alleles; D002681:China; D002999:Clone Cells; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D005854:Germ Cells / Q000166:cytology*; D007795:Laminaria / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny
D018744:DNA, Plant; D005819:Genetic Markers