Preparation and characterization of a novel electrospun spider silk fibroin/poly(D,L-lactide) composite fiber.
In the paper, we successfully prepared spider silk fibroins (Ss)/poly( d, l-lactide) (PDLLA) composite fibrous nonwoven mats for the first time to the best of our knowledge. The morphology of the fibers was observed by a scanning electron microscope (SEM) and transmission electron microscope (TEM). The secondary structure change of the spidroin before and after electrospinning was characterized using Fourier transform infrared spectroscopy (FT-IR). Herein, a qualitative analysis of the conformational changes of the silk protein was performed by analyzing the FT-IR second-derivative spectra, from which quantitative information was obtained via the deconvolution of the amide I band. A mechanical test was carried out to investigate the tensile strength and the elongation at break. A water contact angle (CA) measurement was also performed to characterize surface properties of the fibers. The cytotoxicity of electrospun PDLLA and Ss-PDLLA nonwoven fibrous mats was evaluated based on a CCL 81(Vero) cells proliferation study. The results showed that the hydrophilic and mechanical property of the composite fiber were improved by introducing spidroin.
Zhou
Shaobing
S
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China. shaobingzhou@swjtu.cn
Peng
Hongsen
H
Yu
Xiongjun
X
Zheng
Xiaotong
X
Cui
Wenguo
W
Zhang
Zairong
Z
Li
Xiaohong
X
Wang
Jianxin
J
Weng
Jie
J
Jia
Wenxiang
W
Li
Fei
F
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D005349:Fibroins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D011091:Polyesters / Q000737:chemistry*; D011789:Quantum Theory; D013112:Spiders
D011091:Polyesters; C033616:poly(lactide); D005349:Fibroins