title: Detection by PCR of adhesins genes and slime production in clinical Staphylococcus aureus.
abstract: The presence of the ica loci and adhesins genes in clinical Staphylococcus aureus strains were considered important factors of virulence. In this study, 46 strains of Staphylococcus aureus were isolated from auricular infection, and were investigated for slime production using Congo Red Agar method (CRA). In order to detect the adhesins genes (ica A, ica D, fnb A, cna, Clf A) Polymerase Chain Reaction was used. Qualitative biofilm production of S. aureus using CRA plates revealed that 56.5% of strains were slime producers. In addition 78.26% of strains were ica A and ica D positive. While the fnbA gene was present in 76.1% of isolated strains. Furthermore, 56.5% of strains have the cna gene and 30.4% were clfA positives. Overall this study confirms the presence of fnb A and ica A/ica D genes in the majority of studies S. aureus strains isolated from Staphylococcal sepsis.
authors: Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie, Monastir, Tunisie. zmantar_t@yahoo.fr
authors: Tarek
authors: T
authors: Zmantar
authors: Kamel
authors: K
authors: Chaieb
authors: Héla
authors: H
authors: Makni
authors: Hanene
authors: H
authors: Miladi
authors: Fethi Ben
authors: FB
authors: Abdallah
authors: Kacem
authors: K
authors: Mahdouani
authors: Amina
authors: A
authors: Bakhrouf
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004427:Ear Diseases / Q000382:microbiology; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D018829:Adhesins, Bacterial; D003470:Culture Media; D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial; D037521:Virulence Factors