title: Expression profiling of supraglottic carcinoma: PTEN and thrombospondin 2 are associated with inhibition of lymphatic metastasis.
abstract: CONCLUSIONS
  Down-regulation of metastasis inhibitor genes PTEN and thrombospondin 2 may play a role in the lymphatic metastasis of supraglottic carcinoma.
  
  
  OBJECTIVE
  To investigate differentially expressed genes in supraglottic squamous cell carcinoma with and without lymphatic metastasis using cDNA microarray.
  
  
  METHODS
  cDNA microarray was used to detect the expression profiles of supraglottic carcinoma and the results were validated with real-time PCR.
  
  
  RESULTS
  Compared with normal laryngeal tissues, the expression of 15.6% (15/96) genes including cathepsin B, cathepsin D, cathepsin L, Ets1, c-Ets2, and PEA3 were up-regulated in both cases of supraglottic carcinoma. Compared with the supraglottic carcinoma with no lymph node metastasis, the expression of metastasis inhibitor genes PTEN and thrombospondin 2 was down-regulated in the supraglottic carcinoma tissue with lymph node metastasis.
authors: Department of Otorhinolaryngology, Shenjing Hospital of China Medical University, Shenyang, China. bweiliang@yahoo.com.cn
authors: Weiliang
authors: W
authors: Bai
authors: Liping
authors: L
authors: Wang
authors: Wenyue
authors: W
authors: Ji
authors: Hong
authors: H
authors: Gao
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008207:Lymphatic Metastasis / Q000235:genetics / Q000517:prevention & control; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D019699:Thrombospondins / Q000378:metabolism*; D000099262:Thrombospondin 2
chemical_list: D051059:PTEN Phosphohydrolase; D019699:Thrombospondins; D000099262:Thrombospondin 2; C494929:PTEN protein, human