title: Human dendritic cells differentiated in hypoxia down-modulate antigen uptake and change their chemokine expression profile.
abstract: Dendritic cells (DCs) are the most potent antigen-presenting cells and fine-tune the immune response. We have investigated hypoxia's effects on the differentiation and maturation of DCs from human monocytes in vitro, and have shown that it affects DC functions. Hypoxic immature DCs (H-iDCs) significantly fail to capture antigens through down-modulation of the RhoA/Ezrin-Radixin-Moesin pathway and the expression of CD206. Moreover, H-iDCs released higher levels of CXCL1, VEGF, CCL20, CXCL8, and CXCL10 but decreased levels of CCL2 and CCL18, which predict a different ability to recruit neutrophils rather than monocytes and create a proinflammatory and proangiogenic environment. By contrast, hypoxia has no effect on DC maturation. Hypoxic mature DCs display a mature phenotype and activate both allogeneic and specific T cells like normoxic mDCs. This study provides the first demonstration that hypoxia inhibits antigen uptake by DCs and profoundly changes the DC chemokine expression profile and may have a critical role in DC differentiation, adaptation, and activation in inflamed tissues.
authors: Centre for Experimental Research and Medical Studies, San Giovanni Battista Hospital, Torino, Italy.
authors: Angela Rita
authors: AR
authors: Elia
authors: Paola
authors: P
authors: Cappello
authors: Maura
authors: M
authors: Puppo
authors: Tiziana
authors: T
authors: Fraone
authors: Cristina
authors: C
authors: Vanni
authors: Alessandra
authors: A
authors: Eva
authors: Tiziana
authors: T
authors: Musso
authors: Francesco
authors: F
authors: Novelli
authors: Luigi
authors: L
authors: Varesio
authors: Mirella
authors: M
authors: Giovarelli
mesh_terms: D000941:Antigens / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D015536:Down-Regulation; D005434:Flow Cytometry; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D010641:Phenotype; D010766:Phosphorylation; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism
chemical_list: D000941:Antigens; D018925:Chemokines; D016207:Cytokines; D004268:DNA-Binding Proteins; C086680:ETV5 protein, human; D014157:Transcription Factors; D020741:rho GTP-Binding Proteins