Cyclic diarylheptanoids from Myrica nana inhibiting nitric oxide release.
Investigation of the roots of Myrica nana afforded five new cyclic diarylheptanoids, myricananins A-E (1-5), two new artifacts of myricananins A and B (6-7), and four known compounds, 12-hydroxymyricanone (8), alnusonol (9), myricatomentogenin (10), and actinidione (11). The structures of these new compounds were established by detailed spectroscopic methods. The stereochemistry of compounds 1 and 2 were determined by single-crystal X-ray diffraction. In exception of compounds 2, 6 and 10, all the other compounds were examined for their inhibitory effects on nitric oxide production in lipopolysaccharides-activated macrophages. Compounds 1, 3, 7, 8 and 9 inhibited the release of nitric oxide with IC(50) values of 45.32, 63.51, 52.81, 30.19 and 46.18muM, respectively. Furthermore, compound 1 was found to inhibit the expression of inducible nitric oxide synthase.
Wang
Junfeng
J
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming, Institute of Botany, Chinese Academy of Sciences, Lanhei Road 134, Kunming, 650204 Yunnan, People's Republic of China.
Dong
Shasha
S
Wang
Yuehu
Y
Lu
Qing
Q
Zhong
Huimin
H
Du
Guanhua
G
Zhang
Li
L
Cheng
Yongxian
Y
D036381:Diarylheptanoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides; D008262:Macrophage Activation / Q000187:drug effects* / Q000502:physiology; D031641:Myrica / Q000737:chemistry*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000378:metabolism; D013057:Spectrum Analysis; D014961:X-Ray Diffraction
D036381:Diarylheptanoids; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II