Assessment of the stability of cell-surface components of microorganisms by MALDI-TOF-MS following preservation on lenticule discs.
Strains representing the species Campylobacter coli, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus were randomly selected to assess the consistency of cells preserved on lenticule discs to those archived in traditional freeze-dried ampoules. Each matched pair was cultured using identical conditions and analysed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) to profile the surface-associated molecules of the cells. In addition, the cytosolic/membrane-bound proteins of C. coli and S. aureus strains were further analysed by surface-enhanced laser desorption/ionization time-of-flight MS. The mass spectral profiles in all cases showed a high degree of concordance between cells preserved by both methods and suggest that the properties of cells preserved on lenticule disc are consistent with those archived by the traditional method of freeze-drying.
Shah
Haroun N
HN
Molecular Identification Services Unit, Health Protection Agency Centre for Infections, London, UK. haroun.shah@hpa.org.uk
Molenaar
Linda
L
Rajakaruna
Lakshani
L
Russell
Julie E
JE
Dare
Diane
D
Sutton
Helen
H
Hallas
Gillian
G
D001426:Bacterial Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry*; D005612:Freeze Drying; D006090:Gram-Negative Bacteria / Q000737:chemistry*; D006094:Gram-Positive Bacteria / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis*; D013048:Specimen Handling; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D008565:Membrane Proteins