title: Gene expression analysis defines differences between region-specific GABAergic neurons.
abstract: Gamma-aminobutyric acid (GABA)ergic neurons are a diverse group of inhibitory neurons playing crucial roles in information processing. We analyzed the gene expression of regionally defined GABAergic neurons from the cortex, olfactory bulb, striatum, and cerebellum of glutamate decarboxylase 67-green fluorescence protein (GAD67-GFP) knock-in mice. We introduce a generally applicable method for singularization of brain cells, flow cytometric enrichment, and global mRNA amplification for sensitive gene expression profiling. Systematic quantification elicited a high dynamic range of GABAergic cell numbers in different brain regions. Clustering of our gene expression results revealed major differences between hind and forebrain GABAergic neurons indicating that the development of GABAergic neurons depends on their regional location. While GABAergic neurons of the forebrain are characterized by three main groups of transcription factors of the Distal-less-family, the POU-family, and ETS/FOX family, specific members of the ZIC- and LHX-family of transcription factors appear to define hindbrain inhibitory neurons.
authors: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany. olaf.hardt@miltenyibiotec.de
authors: Olaf
authors: O
authors: Hardt
authors: Corinna
authors: C
authors: Scholz
authors: Daniel
authors: D
authors: Küsters
authors: Yuchio
authors: Y
authors: Yanagawa
authors: Sandra
authors: S
authors: Pennartz
authors: Harold
authors: H
authors: Cremer
authors: Andreas
authors: A
authors: Bosio
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D055879:Gene Knock-In Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005680:gamma-Aminobutyric Acid