Expression of chloride intracellular channel protein 1 (CLIC1) and tumor protein D52 (TPD52) as potential biomarkers for colorectal cancer.
OBJECTIVE
Unequivocal biomarkers are needed to predict susceptibility and progression of colorectal cancer.


METHODS
Paired samples of tumor and normal tissue from six patients with colorectal cancer of different localization, pTNM stage and grade were employed in the present study. MS analysis was used to identify differentially regulated proteins after 2-DE separation and densitometric analysis.


RESULTS
Densitometric analysis revealed differential abundance of 55 spots in tumor as compared to normal tissues. Thirty nine out of 55 spots were unambiguously identified by MS representing 32 different proteins. CLIC1, TPD52 and FABPL were consistently overexpressed (>3-fold, P<0.05) in all tumor tissue samples, while TPM1, TPM2, TPM3, TAGL and MLRN were consistently down-regulated (>3-fold, P<0.05) compared to normal tissue.


CONCLUSIONS
CLIC1 and TPD52 were significantly (P<0.05) up-regulated in all cases of colorectal cancer investigated, irrespective of localization, pTNM stage and grade of colon cancer highlighting their potential to serve as new biomarkers.
Petrova
Darinka Todorova
DT
Department of Medical Genetics, Medical University, Sofia, Bulgaria.
Asif
Abdul R
AR
Armstrong
Victor W
VW
Dimova
Ivanka
I
Toshev
Svetoslav
S
Yaramov
Nikolay
N
Oellerich
Michael
M
Toncheva
Draga
D
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018118:Chloride Channels / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D003710:Demography; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism*; D015203:Reproducibility of Results
D014408:Biomarkers, Tumor; C501966:CLIC1 protein, human; D018118:Chloride Channels; D009363:Neoplasm Proteins; C114751:TPD52 protein, human