title: Expansion of a unique macrophage subset in rheumatoid arthritis synovial lining layer.
abstract: The Z39Ig protein (complement receptor for C3b and iC3b) is expressed on resident tissue macrophages in various tissues. This study was undertaken to examine the distribution of Z39Ig+cells and their phenotypic features in rheumatoid arthritis (RA) synovium, in comparison with those of osteoarthritis (OA) and psoriatic arthritis (PsA) synovium. Monoclonal anti-Z39Ig antibody was produced by immunizing Z39Ig transfected murine pre B cells and used for the identification of Z39Ig+cells. Z39Ig+cells were further stained with antibodies to macrophages, fibroblast-like synoviocytes, complement receptors and dendritic cells by using the double immunostaining method in normal, RA, OA and PsA synovium. RA synovial mononuclear cells were double-stained using anti-Z39Ig and anti-CD11c antibodies and sorted into Z39Ig+CD11c+cells and Z39Ig+CD11c-cells. These cell populations were then analysed by electron microscopy. The expression of the Z39Ig protein was limited to intimal macrophages in normal, RA, OA and PsA synovium. The numbers of Z39Ig+CD11c+cells and the ratios of Z39Ig+CD11c+cells to Z39Ig+cells were increased in the synovial lining layer of RA as compared with those of OA and PsA. The ultrastructural analysis of Z39Ig+CD11c+cells showed the character of macrophages with many secondary lysosomes and swelling of mitochondria. Z39Ig+ cells appeared to be useful for identification of resident tissue macrophages in normal synovium and the corresponding macrophages in the synovial lining layer of inflammatory arthritis. Expansion of Z39Ig+CD11c+cells was characteristic of RA synovial lining layer.
authors: Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
authors: M
authors: M
authors: Tanaka
authors: T
authors: T
authors: Nagai
authors: Y
authors: Y
authors: Tsuneyoshi
authors: N
authors: N
authors: Sunahara
authors: T
authors: T
authors: Matsuda
authors: T
authors: T
authors: Nakamura
authors: S
authors: S
authors: Tsuyama
authors: K
authors: K
authors: Hasui
authors: O
authors: O
authors: FitzGerald
authors: T
authors: T
authors: Matsuyama
mesh_terms: D000328:Adult; D000368:Aged; D015535:Arthritis, Psoriatic / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D039521:CD11c Antigen / Q000276:immunology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D010003:Osteoarthritis / Q000276:immunology; D011951:Receptors, Complement / Q000276:immunology*; D018709:Statistics, Nonparametric; D013583:Synovial Membrane / Q000276:immunology*
chemical_list: D039521:CD11c Antigen; D011951:Receptors, Complement; C414427:VSIG4 protein, human