Effect of root surface treatment with propolis and fluoride in delayed tooth replantation in rats.
Replantation is an acceptable option for treatment of an avulsed permanent tooth. Nevertheless, an extended extraoral period damages the periodontal ligament and results in external root resorption. The purpose of this study was to assess by histologic and histometric analysis, the influence of propolis 15% (natural resinous substance collected by Apis mellifera bees from various plants) and the fluoride solution used as root surface treatment on the healing process after delayed tooth replantation. Thirty Wistar (Rattus norvegicus albinus) rats were submitted to extraction of their upper right incisor. The teeth were maintained in a dry environment for 60 min. After this, the pulp was extirpated and the papilla, enamel organ and periodontal ligament were removed with scalpel. The teeth were divided into three experimental groups: Group I - teeth immersed in 20 ml of physiologic saline; Group II - teeth immersed in 20 ml of 2% acidulated phosphate sodium fluoride; Group III - teeth immersed in 20 ml of 15% propolis. After 10 min of immersion in the solutions, the root canals were dried and filled with calcium hydroxide paste and the teeth were replanted. The animals were euthanized 60 days after replantation. The results showed that similar external root resorption was seen in the propolis and fluoride groups. Teeth treated with physiologic saline tended to have more inflammatory root resorption compared with those treated with fluoride or propolis. However, the comparative analysis did not reveal statistically significant differences (P > 0.05) between the treatment modalities when used for delayed tooth replantation.
Gulinelli
Jéssica Lemos
JL
Department of Surgery and Integrated Clinic, Dental School, São Paulo State University, Araçatuba, Brazil.
Panzarini
Sônia Regina
SR
Fattah
Cristiane Mara Ruiz de Sousa
CM
Poi
Wilson Roberto
WR
Sonoda
Celso Koogi
CK
Negri
Márcia Regina
MR
Saito
Célia Tomiko Matida Hamata
CT
D000149:Acidulated Phosphate Fluoride / Q000627:therapeutic use*; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D002126:Calcium Hydroxide / Q000627:therapeutic use; D003238:Connective Tissue / Q000473:pathology; D003739:Dental Cementum / Q000473:pathology; D003804:Dentin / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D005881:Gingiva / Q000473:pathology; D007180:Incisor / Q000473:pathology; D008297:Male; D010513:Periodontal Ligament / Q000473:pathology; D011429:Propolis / Q000627:therapeutic use*; D020011:Protective Agents / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012184:Retrograde Obturation / Q000379:methods; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D012388:Root Canal Irrigants / Q000627:therapeutic use; D012391:Root Resorption / Q000209:etiology / Q000473:pathology; D020254:Tooth Ankylosis / Q000209:etiology / Q000473:pathology; D014090:Tooth Replantation / Q000379:methods*; D014092:Tooth Root / Q000187:drug effects* / Q000473:pathology; D020390:Tooth Socket / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects; D015034:Zinc Oxide / Q000627:therapeutic use
D020011:Protective Agents; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D000149:Acidulated Phosphate Fluoride; D011429:Propolis; D002126:Calcium Hydroxide; D015034:Zinc Oxide