Decreased total antioxidant capacity in plasma, but not tissue, in experimental colitis.
The aim of the present work was to compare colonic mucosa and plasmatic oxidative stress measured concomitantly and with different degrees of injury in rats with colitis induced by trinitrobenzene sulfonic acid. Three groups were studied: control group, colitis group, and colitis exacerbated by diclofenac. Enzymatic markers of colon injury showed enhanced activity in both groups with colitis. The colitis group treated with diclofenac presented higher colonic damage score than the other groups. In both groups with colitis, higher values of tert butyl hydroperoxide-initiated-chemiluminescence and thiobarbituric acid-reactive substances in tissue and decreased total radical-trapping antioxidant potential (TRAP) levels in plasma were found. In conclusion, independently of the degree of colonic mucosa injury and inflammation, oxidative stress in tissue occurs as a consequence of pro-oxidants increase, and is not explained by a reduction of antioxidant defenses. In both conditions, TRAP determination decreases in plasma, but not in tissue.
Breganó
José Wander
JW
Department of Pathology, Clinical Analysis and Toxicology, University of Londrina, Londrina, Paraná, Brazil.
Dichi
Jane Bandeira
JB
Barbosa
Décio Sabbatini
DS
El Kadri
Mirian Zebian
MZ
Matsuo
Tiemi
T
Rodrigues
Maria Aparecida
MA
Cecchini
Rubens
R
Dichi
Isaias
I
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000201:enzymology / Q000473:pathology; D016861:Cyclooxygenase Inhibitors; D004008:Diclofenac; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid
D000975:Antioxidants; D015415:Biomarkers; D016861:Cyclooxygenase Inhibitors; D004008:Diclofenac; D014302:Trinitrobenzenesulfonic Acid