Bovine SLC11A1 3' UTR SSCP genotype evaluated by a macrophage in vitro killing assay employing a Brucella abortus strain.
The 3' untranslated region (3' UTR) of the bovine natural resistance-associated macrophage gene (NRAMP1 or SLC11A1) was genotyped in Colombian Creole Blanco Orejinegro (BON) (Bos taurus) (n = 140) and Zebu Brahman (Bos indicus) (Z) (n = 20) cattle and their crosses (BON x Zebu Brahman [B x Z] [n = 10]; Zebu Brahman x BON [Z x B] [n = 10]), and in animals from a Holstein x BON (H x B) (n = 10) cross. Direct sequencing and single-strand conformation polymorphism analysis (SSCP) helped in detecting the polymorphic behaviour. The association between resistance to brucellosis infection and SSCP genotype was evaluated using a macrophage in vitro killing assay employing a virulent Brucella abortus strain. The 3' UTR (GT) repeated polymorphism was gentoyped and its association with resistance to brucellosis was evaluated. When all breeds were grouped, a high frequency in the homozygote GT(12) (AA genotype) (0.823) and a very low frequency in the homozygote GT(10) (BB genotype) (0.047) were detected. The BON (0.963), Z x B (0.60) and H x B (1.00) cattle showed high GT(12) allele frequencies, unlike that seen for the B x Z and Zebu cattle (0.3002 and 0.218, respectively). The GT(10) allele was only found in the Zebu cattle (0.391). A significant association (p < 0.001) was found between the B. abortus macrophage in vitro killing assay phenotypes and the bovine SLC11A1 3' UTR genotypes, which suggests that the A allele may be associated with resistance. Because only nine animals had the BB genotype, the results require some confirmation in more extensive populations.
Martinez
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R
Grupo de Recursos Genéticos y Biotecnología Animal, Centro de Biotecnología y Bioindustria, Corporación Colombiana de Investigación Agropecuaria (CORPOICA), CI Tibaitatá Km, Bogotá DC, Colombia. rodrigomartinez19@yahoo.com <rodrigomartinez19@yahoo.com>
Toro
R
R
Montoya
F
F
Burbano
M
M
Tobón
J
J
Gallego
J
J
Dunner
S
S
Cañón
J
J
D020413:3' Untranslated Regions / Q000737:chemistry*; D000818:Animals; D002003:Brucella abortus / Q000472:pathogenicity*; D002007:Brucellosis, Bovine / Q000235:genetics / Q000276:immunology; D027682:Cation Transport Proteins / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D003433:Crosses, Genetic; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics; D008264:Macrophages / Q000502:physiology; D018895:Microsatellite Repeats; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D000099173:Natural Resistance-associated Macrophage Protein 1
D020413:3' Untranslated Regions; D027682:Cation Transport Proteins; D005819:Genetic Markers; D000099173:Natural Resistance-associated Macrophage Protein 1