Profiling of acarviostatin family secondary metabolites secreted by Streptomyces coelicoflavus ZG0656 using ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry.
Profiling of acarviostatin family secondary metabolites secreted by Streptomyces coelicoflavus ZG0656 was performed by means of a rapid and facile procedure using ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC/ESI-MS). The acarviostatins were separated on a C18 UPLC column with a series of acetonitrile-aqueous ammonia gradients. The target homologues were detected using the multiple reaction monitoring mode, and the chemical structures were confirmed by analyzing the diagnostic fragment ions in their MS/MS spectra. All six known reference acarviostatins (I03, II03, II13, II23, III03, IV03) were thus identified. In addition, at least 74 acarviostatin homologues, including 65 novel compounds, were characterized. Some of the features of the novel structures included having up to five acarviosine moieties, an acarviosine moiety at the reducing terminus, or an incomplete acarviosine moiety at the nonreducing terminus. This type of investigation may be useful for researchers who study secondary metabolomics in microorganisms and plants, especially those who perform metabolic profiling of aminooligosaccharides and other natural products with similar structures.
Geng
Peng
P
Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China.
Meng
Xiansheng
X
Bai
Gang
G
Luo
Guoan
G
D000606:Amino Sugars / Q000032:analysis / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid / Q000379:methods*; D006027:Glycosides / Q000032:analysis / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D048372:Streptomyces coelicolor / Q000737:chemistry / Q000378:metabolism*
D000606:Amino Sugars; D006027:Glycosides; D009844:Oligosaccharides; C076099:acarviosine