An electrochemical sensor for pesticide assays based on carbon nanotube-enhanced acetycholinesterase activity.
There has been urgent demand for rapid, sensitive and cost-effective pesticide assay technologies due to the global attention of environmental and food-safety problems. Acetycholinesterase (AChE)-based electrochemical sensors have attracted significant interest toward this goal. In this contribution, we introduced multiwalled carbon nanotubes (MWNTs) into our sensor design, where they played dual enhancement roles; first is that MWNTs loaded on glassy carbon (GC) electrodes significantly increase surface areas, facilitating the electrochemical polymerization of prussian blue (PB), a redox mediator for the electrochemical oxidation of the enzymatic product, thiocholine (TCh). Second, MWNTs enhance the enzymatic activity of AChE, as manifested by the decreased Michaelis-Menten constant (K(m)). As a result of these two important enhancement factors offered by MWNTs, our electrochemical pesticide sensor exhibited rapid response and high sensitivity toward the detection of a series of pesticides. Moreover, we demonstrated that this sensor was stable, reproducible and selective enough for detection in real samples.
Chen
Haode
H
College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Zuo
Xiaolei
X
Su
Shao
S
Tang
Zhuzhao
Z
Wu
Aibo
A
Song
Shiping
S
Zhang
Dabing
D
Fan
Chunhai
C
D000110:Acetylcholinesterase; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis*; D004867:Equipment Design; D037742:Nanotubes, Carbon; D010575:Pesticides / Q000032:analysis*
D004785:Environmental Pollutants; D037742:Nanotubes, Carbon; D010575:Pesticides; D000110:Acetylcholinesterase