A possible inhibitory action of diaminodiphenyl sulfone on tumour necrosis factor-alpha production from activated mononuclear cells on cutaneous lupus erythematosus.
BACKGROUND
We have previously reported that diaminodiphenyl sulfone (DDS; dapsone) can bring about significant clinical improvement in patients with cutaneous lupus erythematosus (CLE). However, the pharmacological actions of DDS in the skin lesion of patients with CLE are still unknown.


OBJECTIVE
In this study, we aimed to clarify the pharmacological actions of DDS on CLE.


METHODS
We conducted an in vitro study to examine the effects of DDS on the mRNA expression and production of cytokines on lipopolysaccharide-stimulated peripheral blood mononuclear cells. Results. DDS suppressed mRNA expression of tumour necrosis factor (TNF)-alpha and significantly decreased the level of TNF-alpha in culture supernatant.


CONCLUSIONS
DDS may exert an anti-inflammatory action by suppressing TNF-alpha produced by activated mononuclear cells.
Abe
M
M
Department of Dermatology, Gunma University Graduate School of Medicine, Maebashi, Japan. masaabe@med.gunma-u.ac.jp
Shimizu
A
A
Yokoyama
Y
Y
Takeuchi
Y
Y
Ishikawa
O
O
D000328:Adult; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002478:Cells, Cultured; D003622:Dapsone / Q000627:therapeutic use*; D003864:Depression, Chemical; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008178:Lupus Erythematosus, Cutaneous / Q000188:drug therapy* / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000235:genetics; D055815:Young Adult
D000893:Anti-Inflammatory Agents; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D003622:Dapsone