title: [Primary study on glycan structure in pathopoiesis mechanism of recurrent respiratory papillomatosis].
abstract: OBJECTIVE
  To compare the molecular basis difference between recurrent respiratory papillomatosis (RRP) and vocal cord polyp, to analyze the expression of glycan structural genes, and to discuss the pathopoiesis mechanism of RRP.
  
  
  METHODS
  The gene expressing profile between the 3 groups papilloma and the vocal cord polyp regarded as normal larynx epithelium were compared using mRNA parallel amplify and the human genome gene expressing microarray. Through cluster analysis, Gene Ontology function gene annotation and path way analysis, the relative gene of RRP and HPV infection were acquired.
  
  
  RESULTS
  According to three microarrays results, total 567 expression changed genes related to HPV induce RRP were acquired. A serial change of glycan structure biosynthesis and degradation pathways was significant. The expression of dolichyl-phosphate mannosyltransferase polypeptide 1 (DPM1), asparagine-linked glycosylation 1 homolog (ALG1), fucosyltransferase 8 (FUT8) and alpha-mannosidase 1A (MAN1A) were regulated and beta-hexosaminidase (HEXB), beta1-galactosidase (GLB1), exostoses 1 (EXT1), fucosyltransferase (FUT) reduced expression and heparan sulfate 3-O-sulfotransferase 1 (HS3ST3A1) increased expression. The two related enzymes of the glycosphingolipids which is the main composed of the cell membrane, beta-3-N-acetylglucosaminyltransferase 4 (B3GNT4) and UDP-glucose ceramide glucosyltransferase (UGCG) increase expression, HEXB and GLB1 reduced expression.
  
  
  CONCLUSIONS
  The alteration of the coding genes of glycan structure biosynthesis and degradation pathways were significantly and characteristically in pathopoiesis mechanism of RRP. This abnormality may be the beginning of tumor form HPV infection.
authors: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education, Beijing 100730, China.
authors: Jun
authors: J
authors: Wang
authors: De-Min
authors: DM
authors: Han
authors: Hong-Wei
authors: HW
authors: Kang
authors: Li-Jing
authors: LJ
authors: Ma
authors: Jing-Ying
authors: JY
authors: Ye
authors: Yang
authors: Y
authors: Xiao
mesh_terms: D000328:Adult; D020869:Gene Expression Profiling; D006017:Glycolipids / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000821:virology; D009843:Oligoribonucleotides / Q000235:genetics; D010212:Papilloma / Q000235:genetics* / Q000473:pathology* / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics; D011127:Polyps / Q000235:genetics / Q000473:pathology / Q000821:virology; D012142:Respiratory Tract Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000821:virology; D014827:Vocal Cords / Q000473:pathology
chemical_list: D006017:Glycolipids; D006023:Glycoproteins; D009843:Oligoribonucleotides