Detection of an antigenic group 2 coronavirus in an adult alpaca with enteritis.
Antigenic group 2 coronavirus was detected in a fecal sample of an adult alpaca by reverse transcription-PCR. The presence of alpaca coronavirus (ApCoV) in the small intestine was demonstrated by immune histochemistry with an antinucleocapsid monoclonal antibody that reacts with group 2 coronaviruses. Other common causes of diarrhea in adult camelids were not detected. We conclude that nutritional stress may have predisposed the alpaca to severe ApCoV infection.
Genova
Suzanne G
SG
Oklahoma Animal Disease Diagnostic Laboratory, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
Streeter
Robert N
RN
Simpson
Katharine M
KM
Kapil
Sanjay
S
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D002161:Camelids, New World / Q000821:virology*; D017934:Coronavirus / Q000145:classification* / Q000302:isolation & purification*; D004751:Enteritis / Q000821:virology*; D005243:Feces / Q000821:virology; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000821:virology; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D012367:RNA, Viral