Antioxidant effect of thiamine on acutely alcoholized rats and lack of efficacy using thiamine or glucose to reduce blood alcohol content.
Although there is no consensus about the use of glucose and thiamine for the treatment of acute ethanol intoxication, this is a routine practice in many countries. Our objective was to determine the efficacy of this treatment and the changes it causes in the antioxidant status of the liver. Male Wistar rats were intoxicated with an ethanol dose of 5 g/kg and divided into three groups: ethanol (EtOH; untreated), EtOH+G (treated with glucose), and EtOH+B1 (treated with thiamine). Blood and urinary ethanol as well as hepatic malondialdehyde, reduced glutathione and vitamin E were determined in all animals. Blood alcohol levels did not differ between groups, although urinary excretion was about four times higher in the group treated with thiamine (EtOH+B1). The malondialdehyde, reduced glutathione and vitamin E values used here as parameters of the antioxidant system of the liver showed improvement for the thiamine-treated group (EtOH+B1). Treatment with glucose or thiamine was ineffective in reducing blood alcohol levels in rats with acute ethanol intoxication. However, the beneficial effect of thiamine as an antioxidant for ethanol metabolism was demonstrated. Further investigations are necessary to clarify the urinary excretion of ethanol reported here for the first time and the possibility of using thiamine as an antioxidant in situations of chronic alcohol use.
Portari
Guilherme V
GV
Nutrition and Metabolism Course, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil. gportari@fmrp.usp.br
Marchini
Julio Sergio
JS
Vannucchi
Helio
H
Jordao
Alceu A
AA
D000435:Alcoholic Intoxication / Q000188:drug therapy; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D002492:Central Nervous System Depressants / Q000493:pharmacokinetics; D000431:Ethanol / Q000493:pharmacokinetics*; D005947:Glucose / Q000494:pharmacology*; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013831:Thiamine / Q000494:pharmacology*; D014810:Vitamin E / Q000378:metabolism
D000975:Antioxidants; D002492:Central Nervous System Depressants; D014810:Vitamin E; D000431:Ethanol; D008315:Malondialdehyde; D005978:Glutathione; D005947:Glucose; D013831:Thiamine