title: Expression of recombinant rat Neurotrophin-3 in Chinese hamster ovary cells.
abstract: The CHO cell line stably producing recombinant rat NT-3 was established. The insertion of rNT-3 cDNA into transferred cell gonome was analyzed with Southern blot. The expressed protein was identified by Dot ELISA (enzyme-linked immunosorbent assay) and Western blot. Western blot showed a clear specific band of about 14 ku for NT-3. The mean level of rNT-3 in four NT-3cDNA/CHO cell lines was about 2 100 ng/10(6) cells/48 h determined by EIA. The conditioned-medium (CM) of NT-3cDNA/CHO cells could promote the fiber outgrowth of the dissociated dorsal root ganglion of 8-day-old chick embryos, which shows a dose-response relationship. A half-maximal concentration of the biological activity (EC50) of the recombinant protein was approximately 16.7 ng/mL. The MoAb 3W3 of NT-3 could neutralize the biological activity of the rNT-3.
authors: Zhujiang Hospital, The First Military Medical University, Guangzhou, China.
authors: A
authors: A
authors: Ji
authors: S
authors: S
authors: Shu
authors: M
authors: M
authors: Li
authors: X
authors: X
authors: Bao
authors: H
authors: H
authors: Zou
authors: Z
authors: Z
authors: Zhang