Evaluation of a capillary blood collection system for screening for hemoglobinopathies in remote areas.
Accurate estimation of hemoglobin (Hbs) A, Hb A(2), Hb F and abnormal Hb is required for diagnosis of hemoglobinopathies and genetic counseling. High pressure liquid chromatography (HPLC) is the most suitable approach available. But for 70% of the rural Indian population, HPLC analysis facilities are not available and screening would require transportation of samples to laboratories in bigger cities. We thus evaluated the feasibility of using a kit designed for measuring Hb A(1c) using capillary blood for collection and preservation of samples over a period of 15 days at different temperatures for screening for hemoglobinopathies. Capillary blood (5 microl) of 90 individuals was collected in the capillary collection system and run on the Variant Hemoglobin Testing System on days 1, 3, 5, 8, 12 and 15 after incubation at 4, 22, 37, 42 and 50 degrees C. The stability of different Hbs varied at different temperatures. The stability was maintained for 12 to 15 days by most of the samples up to 37 degrees C. Hb E was stable for 3 days up to at 37 degrees C and Hb D and Hb Q for 3 days up to 42 degrees C. This capillary blood collection system would have tremendous potential for sample collection and transportation under adverse climatic conditions for screening of hemoglobinopathies in remote areas in different countries.
Colah
R B
RB
National Institute of Immunohaematology (ICMR), Mumbai, India. colahrb@gmail.com
Wadia
M
M
D'Souza
E
E
Sawant
P M
PM
Mohanty
D
D
Mehra
M
M
D000328:Adult; D001800:Blood Specimen Collection / Q000379:methods*; D002196:Capillaries; D006451:Hemoglobin, Sickle / Q000032:analysis; D006453:Hemoglobinopathies / Q000097:blood / Q000175:diagnosis*; D006455:Hemoglobins, Abnormal / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn / Q000097:blood; D015997:Neonatal Screening / Q000295:instrumentation; D011933:Reagent Kits, Diagnostic; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D017086:beta-Thalassemia / Q000175:diagnosis
D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; D011933:Reagent Kits, Diagnostic; C032001:hemoglobin D