Expression of tachykinin receptors inXenopus oocytes injected with poly (A)(+) RNA from cat dorsal root ganglion.
The expression of the types of tachykinin receptors in the dorsal root ganglion (DRG) neurons by means ofXenopus oocyte expressing system was studied. Poly(A)(+) RNAs were extracted from cat cervical and lumbar DRG. Two days after injection of Poly (A)(+) RNAs, the oocytes were recorded with the two-electrode voltage clamp technique. In the oocytes injected with DRG poly(A)(+) RNA, [Sar(9), Met(O(2))(11)]-substance P(Sar -SP, 1 mumol/L), neurokinin A (NKA, 1 mumol/L) or [beta-Ala(8)]-neurokinin A((4-10)) (Ala-NKA, 1 mumol/L) produced an inward current comprising a rapid spike and a long sustained oscillatory component for several minutes. Sar-SP induced response was blocked by NK-1 antagonist L-668, 169 (1 mumol/L), but not by NK-2 antagonist L-659, 877(1mumol/L). In contrast, Ala-NKA and NKA responses were only blocked by L-659, 877. The oocytes injected with DH Poly(A)(+)RNA also responded to Sar-SP and NKA with similar inward currents, which were selectively blocked by L-668, 169 and L-659, 877, respectively. These tachykinins-induced responses had a potent desensitization. The present data indicate expression of NK-1 and NK-2 receptors in DRG neurons, suggesting that there may be tachykinin autoreceptors on the nociceptive primary afferent terminals.
Li
H
H
Shanghai Brain Research Institute, Chinese Academy of Sciences, 200031, Shanghai, China.
Bao
Y
Y
Zhao
Z
Z