title: Anisakis simplex s.l.: Larvicidal activity of various monoterpenic derivatives of natural origin against L3 larvae in vitro and in vivo.
abstract: This paper describes the activity against Anisakis simplex s.l. L(3) larvae of six monoterpenic derivatives obtained from different essential oils, (alpha-pinene, beta-pinene, ocimene, myrcene, geranyl acetate, and cineole). In in vitro assays, alpha-pinene, ocimene and cineole showed high activity at a concentration of 125 microg/mL (48h) but only alpha-pinene and ocimene were active at 62.5 microg/mL. In in vivo assays, L(3) larvae and study compounds were simultaneously administered per os to Wistar rats. The most active compound was alpha-pinene, finding lesions in only 20% of treated rats versus 98% of controls. Further in vivo studies are required to investigate whether addition of these compounds to food could have a prophylactic effect, reducing the pathogenicity of A. simplex s.l. L(3) in humans, and to explore any possible synergy among compounds.
authors: Departamento de Farmacología, Facultad de Farmacia, Universidad de Granada, Campus de Cartuja, 18071 Granada, Spain.
authors: M C
authors: MC
authors: Navarro
authors: M A
authors: MA
authors: Noguera
authors: M C
authors: MC
authors: Romero
authors: M P
authors: MP
authors: Montilla
authors: J M
authors: JM
authors: González de Selgas
authors: A
authors: A
authors: Valero
mesh_terms: D000818:Animals; D017129:Anisakiasis / Q000188:drug therapy* / Q000469:parasitology / Q000517:prevention & control; D017163:Anisakis / Q000187:drug effects*; D000871:Anthelmintics / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D005393:Fish Diseases / Q000188:drug therapy / Q000469:parasitology / Q000517:prevention & control; D048388:Gadiformes / Q000469:parasitology; D007422:Intestines / Q000187:drug effects / Q000469:parasitology; D007814:Larva / Q000187:drug effects; D039821:Monoterpenes / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D021261:Parasitic Sensitivity Tests; D010529:Peritoneal Cavity / Q000469:parasitology; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000187:drug effects / Q000469:parasitology / Q000473:pathology
chemical_list: D000871:Anthelmintics; D039821:Monoterpenes; D009822:Oils, Volatile