title: The intracellular domain of amyloid precursor protein induces neuron-specific apoptosis.
abstract: Although amyloid precursor protein (APP) has central roles in Alzheimer's disease, the physiological functions of this protein have yet to be fully elucidated. APP homologues show significant sequence conservation in the intracellular domain through evolution, which may reflect the functional importance of the intracellular domain of APP (AICD). To examine this possibility, we established embryonic carcinoma P19 cell lines overexpressing AICD. Although neurons could be differentiated from these cell lines with retinoic acid treatment, overexpression of AICD gave rise to neuron-specific cell death. Furthermore, DNA fragmentation was detected and TUNEL-positive cells were also Tuj1-positive neurons. Taken together, we concluded that AICD can induce neuron-specific apoptosis.
authors: Department of Anatomy, Shinshu University, School of Medicine, Matsumoto, Nagano, Japan. kohzona@gipac.shinshu-u.ac.jp
authors: Kohzo
authors: K
authors: Nakayama
authors: Takeshi
authors: T
authors: Ohkawara
authors: Masahiro
authors: M
authors: Hiratochi
authors: Chang-Sung
authors: CS
authors: Koh
authors: Hisashi
authors: H
authors: Nagase
mesh_terms: D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000502:physiology*; D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D006801:Humans; D020287:In Situ Nick-End Labeling; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000502:physiology*; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D016564:Amyloid beta-Protein Precursor; D014212:Tretinoin