Binding of activator SyrM to the site ofnodD3 P1 region ofRhizobium meliloti.
The nodD3 gene ofRhizobium meliloti is transcribed via promoter P1 or P2. Gel retardation assay showed binding of SyrM to the P1 upstream region of nodD3. DNaseI footprint analysis demonstrated that the binding site of SyrM in nodD3 P1 region consists of two inverted repeat sequences arranged in tandem. SyrM seems to bind to DNA in the form of dimer or tetramer and requires the two inverted repeat sequences for binding.
Xiao
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Laboratory of Molecular Genetics, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, 200032, Shanghai, China.
Shen
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Zhu
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