Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo.
This pilot study examines noninvasive MR monitoring of tissue-engineered vascular grafts (TEVGs) in vivo using cells labeled with iron oxide nanoparticles. Human aortic smooth muscle cells (hASMCs) were labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. The labeled hASMCs, along with human aortic endothelial cells, were incorporated into eight TEVGs and were then surgically implanted as aortic interposition grafts in a C.B-17 SCID/bg mouse host. USPIO-labeled hASMCs persisted in the grafts throughout a 3 wk observation period and allowed noninvasive MR imaging of the human TEVGs for real-time, serial monitoring of hASMC retention. This study demonstrates the feasibility of applying noninvasive imaging techniques for evaluation of in vivo TEVG performance.
Nelson
G N
GN
Yale University School of Medicine, Interdepartmental Program in Vascular Biology and Therapeutics, Amistad Research Bldg., 10 Amistad St., Rm. 301B, P.O. Box 208089, New Haven, CT 06520, USA.
Roh
J D
JD
Mirensky
T L
TL
Wang
Y
Y
Yi
T
T
Tellides
G
G
Pober
J S
JS
Shkarin
P
P
Shapiro
E M
EM
Saltzman
W M
WM
Papademetris
X
X
Fahmy
T M
TM
Breuer
C K
CK
D000818:Animals; D001011:Aorta / Q000166:cytology*; D001807:Blood Vessel Prosthesis; D003287:Contrast Media / Q000494:pharmacology*; D003911:Dextrans; D052203:Ferrosoferric Oxide; D006801:Humans; D007501:Iron / Q000494:pharmacology*; D018810:Magnetic Resonance Angiography / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D016513:Mice, SCID; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D053758:Nanoparticles; D010087:Oxides / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*
D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; C097921:ferumoxtran-10; D007501:Iron; D052203:Ferrosoferric Oxide