Expression of truncated and fused green fluorescent protein genes and analysis of their properties.
The green fluorescent protein (GFP) of the jellyfishAequorea victoria is an unusual protein with strong visible absorbance and fluorescence from a chromophore, which is generated by cyclization and oxidation of the protein's own Ser-Tyr-Gly sequence at position 65-67. The mutant of Ser65 to Thr gives fluorescence intensity sixfold greater than that of the wild type. Several expression vectors containing different truncated lengths or chimericgfp genes were introduced. The relationship between the structure and fluorescence properties of GFP was discussed.
Yue
L
L
Institute of Virology, Wuhan University, 430072, Wuhan, China.
Qi
Y
Y
Hu
J
J
Deng
X
X