title: Exploration of the genes responsible for unlimited proliferation of immortalized lung fibroblasts.
abstract: Regulation mechanism of lung fibroblast proliferation remains unknown. To elucidate the key molecules in it, the authors here established mortal and immortal nontransformed lung fibroblast cell line/strains with elongated life span by telomerase reverse transcriptase gene transfection. Comparing the expression profiles of them, 51 genes were explored to be the candidates responsible for regulation of cellular proliferation of lung fibroblasts. This set of fibrobrast strains of same origin with different proliferative capacities may become useful model cells for research on lung fibroblast growth regulation and the candidate genes explored in this study may provide biomarkers or therapeutic targets of pulmonary fibrosis.
authors: Department of Translational Cancer Research, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan. khiyama@hiroshima-u.ac.jp
authors: Keiko
authors: K
authors: Hiyama
authors: Keiji
authors: K
authors: Tanimoto
authors: Youhei
authors: Y
authors: Nishimura
authors: Momomi
authors: M
authors: Tsugane
authors: Ikuko
authors: I
authors: Fukuba
authors: Yusuke
authors: Y
authors: Sotomaru
authors: Eiso
authors: E
authors: Hiyama
authors: Masahiko
authors: M
authors: Nishiyama
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D002472:Cell Transformation, Viral / Q000235:genetics; D005347:Fibroblasts / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008168:Lung / Q000196:embryology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009374:Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D011003:Ploidies; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D016615:Telomere / Q000235:genetics; D013997:Time Factors; D014162:Transfection
chemical_list: D000952:Antigens, Polyomavirus Transforming; C509186:TERT protein, human; D019098:Telomerase