Expression of the dengue virus type 2 NS5 protein in a CD4(+) T cell line inhibits HIV replication.
Human immunodeficiency virus (HIV) load is suppressed during dengue virus infection. The NS5A phosphoprotein of GB virus C (a related flavivirus) inhibits HIV replication in vitro. To determine whether the dengue virus NS5 protein inhibits HIV replication, CD4(+) T cell lines expressing this protein were generated. HIV replication in dengue virus NS5-expressing cells decreased by >90% compared with control cells (P < .01), and this was mediated in part by decreased HIV coreceptor (CXCR4) expression and increased production of SDF-1. Thus, the dengue virus NS5 protein inhibits HIV replication in vitro, potentially explaining the reduction in HIV load observed during acute dengue virus infection.
McLinden
James H
JH
Iowa City VA Medical Center, 601 Highway 6 West, Iowa City, IA 52246, USA.
Stapleton
Jack T
JT
Chang
Qing
Q
Xiang
Jinhua
J
D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D002460:Cell Line; D018388:Codon, Terminator; D003716:Dengue Virus / Q000235:genetics*; D006678:HIV / Q000502:physiology*; D015497:HIV-1 / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D019169:Jurkat Cells; D010957:Plasmids; D019713:Receptors, CCR5 / Q000502:physiology; D019718:Receptors, CXCR4 / Q000502:physiology; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D014779:Virus Replication / Q000502:physiology*
C527276:CXCR4 protein, human; D018388:Codon, Terminator; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D017361:Viral Nonstructural Proteins