A novel toxin-antitoxin operon talA/B from the Gram-positive bacterium Leifsonia xyli subsp. cynodontis.
Here we report a toxin-antitoxin (TA) operon talAB identified from the Gram-positive bacterium Leifsonia xyli subsp. cynodontis. It is shown that talB encodes a broad-host cytotoxin functioning in different Gram-positive bacteria, while talA encodes its antidote. TalA and TalB form different hetero-oligomers in vitro; these hetero-oligomers, but not the antitoxin TalA, strongly bind to the talAB promoter region containing two inverted repeats. This represents a new mechanism of binding the promoter of a TA operon by the toxin and antitoxin complexes.
Cheng
Lin
L
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei 430072, China.
Lin
Hui
H
Fan
Xuelian
X
Qiu
Shi
S
Sun
Tao
T
Li
Tai-Yuan
TY
Zhang
Yi
Y
D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism; D000992:Antitoxins / Q000235:genetics* / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D000992:Antitoxins; D001427:Bacterial Toxins