Molecular cloning, sequence analysis and expression studies of a novel GAmyb homologous gene, hvmyb.
Using a known GAmyb gene as the probe, two fully identical clones were isolated from a barley aleurone cDNA library. Sequence analysis showed that their 5' termini are highly homologous to the 3' termini of GAmyb (97%) and their 3' termini share no significant homology with any myb genes. Therefore, the deduced protein may hold intact putative GAmyb activation domain but lack the normal DNA-binding domain. Northern blot reveals that hvmyb expression in barley aleurone layers is strongly up-regulated by gibberellin (GA) and down-regulated by abscisic acid (APIA). The tissue-and developmental-stage-specificity of hvmyb was also found, which was only expressed in barley aleurone cells and dropped to non-detectable level soon after germination.
Ling
S
S
Peking University, Beijing, China.
Qi
F
F
Zhu
Y
Y