title: Molecular characterization and expression pattern of the porcine STARS, a striated muscle-specific expressed gene.
abstract: STARS (striated muscle activator of Rho signaling) promotes the nuclear localization of MRTFs and mediates SRF transcription, which provides a potential muscle-specific mechanism for linking changes in the actin cytoskeleton structure with muscle gene expression. In this study, the full-length cDNA of the porcine STARS was cloned. The open reading frame of this gene contains 1,155 bp and encodes a protein of 384 amino acids, which is 79, 73, and 77% identical with human, mouse, and rat STARS genes, respectively. RT-PCR revealed that STARS is specifically expressed in heart and skeletal muscles. STARS is also distinctly different in different muscle developmental stages. The result indicates that its expression increased gradually from 33 dpc (days postcoitum) to postnatal muscles, and peaked 28 days postnatal. The porcine STARS was mapped to SSC4p13 using the somatic cell hybrid panel and the radiation hybrid panel IMpRH (LOD score 11.98). The data show that STARS is closely linked to marker SW871. A T/G single nucleotide polymorphism in the coding sequence, detected as Bsh1236I PCR-RFLP, displays allele frequency differences in six pig breeds.
authors: Laboratory of Molecular Biology and Animal Breeding, College of Animal Science and Technology, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
authors: Y B
authors: YB
authors: Peng
authors: H P
authors: HP
authors: Guan
authors: B
authors: B
authors: Fan
authors: S H
authors: SH
authors: Zhao
authors: X W
authors: XW
authors: Xu
authors: K
authors: K
authors: Li
authors: M J
authors: MJ
authors: Zhu
authors: M
authors: M
authors: Yerle
authors: B
authors: B
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D016366:Open Reading Frames; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: C464441:ABRA protein, human; D008840:Microfilament Proteins; D009124:Muscle Proteins; D014157:Transcription Factors