Acute effects of hyperglycaemia on plasma concentration of soluble P-selectin and von Willebrand factor in healthy volunteers -a prospective randomised double blind controlled study.
This study set out to clarify if isolated hyperglycaemia may directly induce acute endothelial and/or platelet activation as diabetes mellitus is a major risk factor for cardio- and cerebrovascular diseases with thromboembolic complications. 12 healthy volunteers were investigated in a prospective randomised, double blind, three-way cross-over trial with a wash-out period of 21 days. Normoglycemic (4.72 mmol/L), moderate (11.1 mmol/L) or high grade (16.7 mmol/L) glucose clamps were maintained for 6 hours by infusion of glucose, insulin and somatostatin. Volunteers were observed for 24 hours. An increase in soluble (s)P-selectin and von Willebrand Factor (VWF) concentrations, of 26+/-15% and 21+/-7%, respectively was observed 24 hours after euglycaemic treatment, of 20+/-7% and 19+/-5%, respectively after moderate hyperglycaemia and of 22+/-13% and 18+/-7%, respectively after high grade hyperglycaemia at 24 hours (p<0.6 and p<0.004 in all periods and p=0.2-0.9 between periods). In conclusion, acute hyperglycaemia for 6 hours does not increase the platelet and endothelial activation markers sP-selectin and VWF in healthy volunteers.
Kotzailias
Nicole
N
Department of Clinical Pharmacology, Medical University of Vienna, Austria.
Graninger
Monika
M
Knechtelsdorfer
Maarten
M
Jilma
Bernd
B
D000328:Adult; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000097:blood / Q000209:etiology; D002561:Cerebrovascular Disorders / Q000097:blood / Q000209:etiology; D048909:Diabetes Complications / Q000097:blood / Q000209:etiology; D004311:Double-Blind Method; D015309:Glucose Clamp Technique; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006943:Hyperglycemia / Q000097:blood*; D008297:Male; D019007:P-Selectin / Q000097:blood*; D010976:Platelet Count; D011446:Prospective Studies; D012307:Risk Factors; D012995:Solubility; D014841:von Willebrand Factor / Q000378:metabolism*
D015415:Biomarkers; D006454:Hemoglobins; D019007:P-Selectin; D014841:von Willebrand Factor