Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.
Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.
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Institut Pasteur de Nouvelle-Calédonie, Laboratoire d'Epidémiologie Moléculaire, Nouméa, New Caledonia.
Vernel-Pauillac
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O'Connor
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Bremont
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Charavay
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Courvalin
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Goarant
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Le Hello
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D019943:Amino Acid Substitution; D001483:Base Sequence; D027081:DNA Gyrase / Q000737:chemistry / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000737:chemistry / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology; D005798:Genes, Bacterial; D006801:Humans; D009691:Nucleic Acid Denaturation; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D013816:Thermodynamics
D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial; D024841:Fluoroquinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase