Nebrodeolysin, a novel hemolytic protein from mushroom Pleurotus nebrodensis with apoptosis-inducing and anti-HIV-1 effects.
A novel hemolysin was isolated from the edible mushroom Pleurotus nebrodensis by ion exchange and gel filtration chromatography on DEAE-Sepharose and Sephacryl S-100. The hemolysin from Pleurotus nebrodensis hemolysin (nebrodeolysin) is a monomeric protein with a molecular weight of approximately 27 kDa as determined by gel filtration and SDS-PAGE. Nebrodeolysin exhibited remarkable hemolytic activity towards rabbit erythrocytes and caused efflux of potassium ions from erythrocytes. Subsequently, this hemolysin showed strong cytotoxicity against Lu-04, Bre-04, HepG2, L929, and HeLa cells. It was also found that this hemolysin induced apoptosis in L929 and HeLa cells as evidenced by microscopic observations and DNA ladder, respectively. Moreover, this hemolysin was shown to possess anti-HIV-1 activity in CEM cell culture.
Lv
Hui
H
College of Life Sciences, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064, China.
Kong
Yang
Y
Yao
Qing
Q
Zhang
Bo
B
Leng
Fang-Wei
FW
Bian
He-Jiao
HJ
Balzarini
Jan
J
Van Damme
Els
E
Bao
Jin-Ku
JK
D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D048690:Fruiting Bodies, Fungal; D005656:Fungal Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D015658:HIV Infections / Q000188:drug therapy*; D015497:HIV-1 / Q000187:drug effects; D006460:Hemolysin Proteins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D053878:Hemolytic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy*; D008517:Phytotherapy; D020076:Pleurotus / Q000737:chemistry*; D011817:Rabbits
D019380:Anti-HIV Agents; D000970:Antineoplastic Agents; D005656:Fungal Proteins; D006460:Hemolysin Proteins; D053878:Hemolytic Agents