Recombinant IgY for improvement of immunoglobulin-based analytical applications.
OBJECTIVE
In order to provide superior tools for diagnostic approaches and to prevent assay interference and background binding, the objective of this study was the establishment and evaluation of monoclonal IgY which are phylogenetically distant from mammalian immunoglobulins but have been unavailable so far.


METHODS
Human, murine and avian monoclonal model antibodies were established and produced in mammalian cells. Their interaction with human serum components and Fc gamma receptors was compared by ELISA and fluorescence activated cell sorting (FACS).


RESULTS
The use of monoclonal IgY in contrast to mammalian antibodies prevented interference phenomena in absorbance measurements generated by human sera containing rheumatoid factor (RF) or heterophilic antibodies. Additionally, monoclonal IgY exhibited no interaction with the human and murine high-affinity receptor FCGR1 (CD64) and human low affinity receptor FCGR3a (CD16A).


CONCLUSIONS
The data obtained demonstrate the advantageous behaviour of monoclonal IgY as detection or capture antibodies compared to conventional mammalian immunoglobulins and provide a strategy for improvement of assay performance and accuracy.
Greunke
Kerstin
K
Institute of Biochemistry and Food Sciences, Division of Biochemistry and Molecular Biology University of Hamburg, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany.
Braren
Ingke
I
Alpers
Iris
I
Blank
Simon
S
Sodenkamp
Jan
J
Bredehorst
Reinhard
R
Spillner
Edzard
E
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001717:Birds; D002460:Cell Line; D019281:Dimerization; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards*; D007074:Immunoglobulin G / Q000737:chemistry; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000737:chemistry; D051379:Mice; D017452:Receptors, IgG / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D012217:Rheumatoid Factor / Q000276:immunology; D012995:Solubility
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; C496124:FCGR3A protein, human; C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D017452:Receptors, IgG; D011994:Recombinant Proteins; D012217:Rheumatoid Factor