A proteomic approach to differentiate histologically classified stable and unstable plaques from human carotid arteries.
OBJECTIVE
By using proteomics we isolated and identified proteins that were expressed/retained in stable and unstable human carotid artery atherosclerotic plaques.


METHODS
The criteria for plaque instability were the presence of a thin fibrous cap or fissured cap covering the foamy or necrotic core, and the presence of overt, hemorrhagic, ulcerated or thrombotic plaques. Proteins were extracted from finely minced endarterectomy specimens (19 stable and 29 unstable plaques) and separated by two-dimensional gel electrophoresis. Coomassie Blue-stained gels were analysed using PD-Quest software.


RESULTS
A total of 57 distinct spots corresponding to 33 different proteins were identified by matrix assisted laser desorption/ionization mass spectrometry using the NCBI database. Most of the spots were present in both types of extracts, although significantly (p<0.05) differing in abundance between them. Compared to stable plaque, unstable ones showed reduced abundance of: protective enzymes SOD3 and GST, small heat shock proteins HSP27 and HSP20, annexin A10, and Rho GDI. In unstable plaques the more abundant proteins were: ferritin light subunit, SOD 2 and fibrinogen fragment D. For fibrinogen fragment D, the increased levels in unstable versus stable plaques was confirmed by Western blot analysis.


CONCLUSIONS
Since many of the differentially expressed proteins are known to have a functional role in inflammation and oxidative stress, we speculate that they may be involved in events relating to plaque stability.
Lepedda
Antonio J
AJ
Dipartimento di Scienze Fisiologiche, Biochimiche e Cellulari, Università di Sassari, Via Muroni 25, 07100 Sassari, Italy.
Cigliano
Antonio
A
Cherchi
Gian Mario
GM
Spirito
Rita
R
Maggioni
Marco
M
Carta
Franco
F
Turrini
Franco
F
Edelstein
Celina
C
Scanu
Angelo M
AM
Formato
Marilena
M
D050197:Atherosclerosis / Q000175:diagnosis / Q000473:pathology*; D002339:Carotid Arteries / Q000473:pathology*; D016893:Carotid Stenosis / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007249:Inflammation; D008954:Models, Biological; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D018384:Oxidative Stress; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D012394:Rosaniline Dyes / Q000494:pharmacology; D015398:Signal Transduction; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D012394:Rosaniline Dyes; C048139:Coomassie blue