Synthesis of toll-like receptor-2 targeting lipopeptides as self-adjuvanting vaccines.
Effective Th1- and Th2-type immune responses that result in protective immunity against pathogens can be induced by self-adjuvanting lipopeptides containing the lipid moiety dipalmitoyl-S-glyceryl cysteine (Pam2Cys). The potent immunogenicity of these lipopeptides is due to their ability to activate dendritic cells by targeting and signaling through Toll-like receptor-2 (TLR-2). In addition, the simplicity and flexibility in their design as well as their ease of chemical definition and characterisation makes them highly attractive vaccine candidates for humans and animals. We describe in this chapter the techniques involved in the synthesis of an immunocontraceptive lipopeptide vaccine as well as the experimental assays carried out to evaluate its efficiency.
Chua
Brendon Y
BY
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia.
Zeng
Weiguang
W
Jackson
David C
DC
D000276:Adjuvants, Immunologic / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D003270:Contraceptive Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D000939:Epitopes; D005260:Female; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D011247:Pregnancy; D051195:Toll-Like Receptor 2 / Q000276:immunology*; D014612:Vaccines / Q000737:chemistry / Q000276:immunology
D000276:Adjuvants, Immunologic; D003270:Contraceptive Agents; D000939:Epitopes; D008055:Lipids; D010455:Peptides; D051195:Toll-Like Receptor 2; D014612:Vaccines