An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus.
The salivary glands of blood-sucking arthropods contain a redundant 'magic potion' that counteracts their vertebrate host's hemostasis, inflammation, and immunity. We here describe the salivary transcriptome and proteomics (sialome) of the soft tick Ornithodoros coriaceus. The resulting analysis helps to consolidate the classification of common proteins found in both soft and hard ticks, such as the lipocalins, Kunitz, cystatin, basic tail, hebraein, defensin, TIL domain, metalloprotease, 5'-nucleotidase/apyrase, and phospholipase families, and also to identify protein families uniquely found in the Argasidae, such as the adrenomedullin/CGRP peptides, 7DB, 7 kDa, and the RGD-containing single-Kunitz proteins. Additionally, we found a protein belonging to the cytotoxin protein family that has so far only been identified in hard ticks. Three other unique families common only to the Ornithodoros genus were discovered. Edman degradation, 2D and 1D-PAGE of salivary gland homogenates followed by tryptic digestion and HPLC MS/MS of results confirms the presence of several proteins. These results indicate that each genus of hematophagous arthropods studied to date evolved unique protein families that assist blood feeding, thus characterizing potentially new pharmacologically active components or antimicrobial agents.
Francischetti
Ivo M B
IM
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892-8132, USA. ifrancischetti@niaid.nih.gov
Meng
Zhaojing
Z
Mans
Ben J
BJ
Gudderra
Nanda
N
Hall
Mark
M
Veenstra
Timothy D
TD
Pham
Van M
VM
Kotsyfakis
Michail
M
Ribeiro
José M C
JM
D000022:Abortion, Spontaneous / Q000469:parasitology*; D053607:Adrenomedullin / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D017124:Conserved Sequence; D005260:Female; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D020543:Proteome; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D016415:Sequence Alignment; D013984:Tick Infestations / Q000662:veterinary*; D013987:Ticks / Q000235:genetics* / Q000472:pathogenicity; D014357:Trypsin
D020543:Proteome; D012471:Salivary Proteins and Peptides; D053607:Adrenomedullin; D002116:Calcitonin; D014357:Trypsin