Renal glomerulogenesis in medaka fish, Oryzias latipes.
We provide an overview of glomerulogenesis in medaka from the embryo to the adult by means of in situ hybridization with the wt1 gene as a marker as well as histology and three-dimensional images. The pronephric glomus starts to develop in the intermediate mesoderm during early somitogenesis, is completed before hatching, and persists throughout the lifetime of the fish. Within 5 days after hatching, mesonephric glomerulus formation begins in the caudomedial end of the pronephric sinus and duct area. The number of glomeruli reaches approximately 200-300 in each kidney within 2 months after hatching. wt1 expression during nephron maturation served as a marker for the formation of the mesenchymal condensate and the nephrogenic body. Existence of mesenchymal condensates and persistence of wt1 expression in the adult kidney suggest that the mesonephros retains precursor cells that may be capable of contributing to neoglomerulogenesis during adulthood.
Fedorova
Svetlana
S
Laboratory of Freshwater Fish Stocks, Bioscience and Biotechnology Center, Nagoya University, Nagoya, Japan.
Miyamoto
Rieko
R
Harada
Tomohiro
T
Isogai
Sumio
S
Hashimoto
Hisashi
H
Ozato
Kenjiro
K
Wakamatsu
Yuko
Y
D000818:Animals; D029941:Fish Proteins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D007678:Kidney Glomerulus / Q000196:embryology* / Q000378:metabolism; D009399:Nephrons / Q000196:embryology / Q000378:metabolism; D009990:Oryzias / Q000196:embryology* / Q000378:metabolism; D025721:WT1 Proteins / Q000235:genetics
D029941:Fish Proteins; D025721:WT1 Proteins