title: T cells' immunological synapses induce polarization of brain astrocytes in vivo and in vitro: a novel astrocyte response mechanism to cellular injury.
abstract: BACKGROUND
  Astrocytes usually respond to trauma, stroke, or neurodegeneration by undergoing cellular hypertrophy, yet, their response to a specific immune attack by T cells is poorly understood. Effector T cells establish specific contacts with target cells, known as immunological synapses, during clearance of virally infected cells from the brain. Immunological synapses mediate intercellular communication between T cells and target cells, both in vitro and in vivo. How target virally infected astrocytes respond to the formation of immunological synapses established by effector T cells is unknown.
  
  
  RESULTS
  Herein we demonstrate that, as a consequence of T cell attack, infected astrocytes undergo dramatic morphological changes. From normally multipolar cells, they become unipolar, extending a major protrusion towards the immunological synapse formed by the effector T cells, and withdrawing most of their finer processes. Thus, target astrocytes become polarized towards the contacting T cells. The MTOC, the organizer of cell polarity, is localized to the base of the protrusion, and Golgi stacks are distributed throughout the protrusion, reaching distally towards the immunological synapse. Thus, rather than causing astrocyte hypertrophy, antiviral T cells cause a major structural reorganization of target virally infected astrocytes.
  
  
  CONCLUSIONS
  Astrocyte polarization, as opposed to hypertrophy, in response to T cell attack may be due to T cells providing a very focused attack, and thus, astrocytes responding in a polarized manner. A similar polarization of Golgi stacks towards contacting T cells was also detected using an in vitro allogeneic model. Thus, different T cells are able to induce polarization of target astrocytes. Polarization of target astrocytes in response to immunological synapses may play an important role in regulating the outcome of the response of astrocytes to attacking effector T cells, whether during antiviral (e.g. infected during HIV, HTLV-1, HSV-1 or LCMV infection), anti-transplant, autoimmune, or anti-tumor immune responses in vivo and in vitro.
authors: Board of Governors' Gene Therapeutics Research Institute, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, California, United States of America.
authors: Carlos
authors: C
authors: Barcia
authors: Nicholas S R
authors: NS
authors: Sanderson
authors: Robert J
authors: RJ
authors: Barrett
authors: Kolja
authors: K
authors: Wawrowsky
authors: Kurt M
authors: KM
authors: Kroeger
authors: Mariana
authors: M
authors: Puntel
authors: Chunyan
authors: C
authors: Liu
authors: Maria G
authors: MG
authors: Castro
authors: Pedro R
authors: PR
authors: Lowenstein
mesh_terms: D000256:Adenoviridae; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D001921:Brain / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology; D018920:Coculture Techniques; D003342:Corpus Striatum / Q000187:drug effects / Q000502:physiology / Q000821:virology; D008297:Male; D018613:Microscopy, Confocal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000502:physiology / Q000821:virology; D013569:Synapses / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology*; D013937:Thymidine Kinase / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D013937:Thymidine Kinase