OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models.
OBJECTIVE
To establish nude mouse human gastric cancer orthotopic transplantation models using OB glue paste technique.


METHODS
Using OB glue paste technique, orthopic transplantation models were established by implanting SGC-7901 and MKN-45 human gastric cancer cell strains into the gastric wall of nude mice. Biological features, growth of the implanted tumors, the success rate of transplantation and the rate of auto-metastasis of the two models were observed.


RESULTS
The success rates of orthotopic transplantation of the two models were 94.20% and 96%. The rates of hepatic metastasis, pulmonary metastasis, peritoneal metastasis, lymphocytic metastasis and splenic metastasis were 42.13% and 94.20%, 48.43% and 57.97%, 30.83% and 36.96%, 67.30% and 84.06%, and 59.75% and 10.53%, respectively. The occurrence of ascites was 47.80% and 36.96%.


CONCLUSIONS
OB glue paste technique is easy to follow. The biological behaviors of the nude mouse human gastric cancer orthotopic transplantation models established with this technique are similar to the natural processes of growth and metastasis of human gastric cancer, and, therefore, can be used as an ideal model for experimental research of proliferative metastasis of tumors.
Shi
Jun
J
Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, Shanghai 200003, China.
Wei
Pin-Kang
PK
Zhang
Shen
S
Qin
Zhi-Feng
ZF
Li
Jun
J
Sun
Da-Zhi
DZ
Xiao
Yan
Y
Yu
Zhi-Hong
ZH
Lin
Hui-Ming
HM
Zheng
Guo-Jing
GJ
Su
Xiao-Mei
XM
Chen
Ya-Lin
YL
Liu
Yan-Fang
YF
Xu
Ling
L
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D013270:Stomach / Q000473:pathology / Q000601:surgery*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology / Q000601:surgery*; D014014:Tissue Adhesives; D014183:Transplantation, Heterologous; D018615:Ultrasonography, Doppler, Color
D014014:Tissue Adhesives