Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood.
Plasma aromatic and sulfur containing amino acids are good indicators of protein anabolism/catabolism, while blood reduced and oxidized glutathione reflect oxidative status in an organism. Using a full factorial design for screening important variables (pH, concentration, temperature) we developed a capillary zone electrophoresis method permitting their measurements in the single run, without any derivatization procedures. The best separations were obtained within less than 30 min employing a 10 mmol/l phosphate buffer, pH 2.8, 18 degrees C, 15 kV voltage. Fairly good precision with a linear relationship between peak area and concentrations (r=0.995-0.999) were obtained. The method was used to analyze human capillary blood.
Zunić
Gordana
G
Institute for Medical Research, Military Medical Academy, Belgrade, Serbia. slavica@pharmacy.bg.ac.yu
Spasić
Slavica
S
D000596:Amino Acids / Q000097:blood* / Q000302:isolation & purification; D002196:Capillaries; D019075:Electrophoresis, Capillary; D005978:Glutathione / Q000097:blood* / Q000302:isolation & purification; D019803:Glutathione Disulfide / Q000097:blood; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000596:Amino Acids; D005978:Glutathione; D019803:Glutathione Disulfide