Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes.
Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of transgenic plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties.
Lü
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Institute of Genetics, Chinese Academy of Sciences, Beijing, China.
Cao
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Tang
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Tian
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