Ascorbic acid combats arsenic-induced oxidative stress in mice liver.
Repeated injections of arsenic trioxide induced oxidative stress and hepatotoxicity in mice as revealed from elevated levels of glutamate oxaloacetate transaminases, glutamate pyruvate transaminases, acid and alkaline phosphatases, lipid peroxidation along with reduction of superoxide dismutase, catalase, reduced glutathione content, glutathione reductase and succinate dehydrogenase activities. The present investigation was undertaken to test whether simultaneous feeding of vitamin C can combat hepatotoxicity in arsenic intoxicated mice. Hepatoprotective potential of vitamin C was indicated by its ability to restore GSH, SOD, CAT, AcP, AlkP and GRD levels towards near normal. Electron microscopic studies further supported the biochemical findings confirming the hepatoprotective potential of ascorbic acid. Besides, cytogenetical endpoints (chromosome aberrations, micronuclei, mitotic index and sperm head anomaly) were also analyzed. Administration of vitamin C alone did not show any sign of toxicity of its own. Based on the present findings, ascorbic acid appears to have protective effects against arsenic toxicity and oxidative stress.
Banerjee
Pathikrit
P
Department of Zoology, University of Kalyani, Kalyani-741235, West Bengal, India.
Bhattacharyya
Soumya Sundar
SS
Bhattacharjee
Nandini
N
Pathak
Surajit
S
Boujedaini
Naoual
N
Belon
Philippe
P
Khuda-Bukhsh
Anisur Rahman
AR
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001151:Arsenic / Q000633:toxicity*; D020261:Arsenic Poisoning / Q000188:drug therapy / Q000517:prevention & control; D001205:Ascorbic Acid / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis; D004789:Enzyme Activation / Q000187:drug effects* / Q000502:physiology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D015162:Micronucleus Tests; D008854:Microscopy, Electron; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology
D000975:Antioxidants; D015415:Biomarkers; D001151:Arsenic; D001205:Ascorbic Acid