Cell-cycle deregulation induced by three different root canal sealers in vitro.
The aim of this study was to investigate the effects of 3 root canal sealers on the cell cycle of mouse fibroblasts (3T3 cell line). Freshly mixed root canal sealers (AH Plus, Endofill, and Sealer 26) were eluted for 24 h using cell culture medium. Antiproliferation activity of different eluate dilutions was determined by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. Cell cycle was subsequently evaluated by flow cytometry. Differences were tested by analysis of variance with Tukey test (P < .05). Results showed that the root canal sealers inhibited cell growth in a dose-dependent manner. Antiproliferation activity of the root canal sealers was associated with cell-cycle deregulation. This event involved cell-cycle arrest in the S/G2/M phases and an increase of the sub-G1 population, which are consistent with both cytostatic and cytotoxic effects. This information may contribute to a better understanding of the toxicity mechanism displayed by root canal sealers currently used in clinical practice.
Valois
Caroline R A
CR
Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasília, Brasília, Brazil. cravalois@uol.com.br
Azevedo
Ricardo B
RB
D016475:3T3 Cells; D000818:Animals; D001729:Bismuth / Q000633:toxicity; D002126:Calcium Hydroxide / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004853:Epoxy Resins / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects*; D005434:Flow Cytometry; D008422:Materials Testing; D051379:Mice; D012387:Root Canal Filling Materials / Q000633:toxicity*
D004853:Epoxy Resins; D012387:Root Canal Filling Materials; C080620:Sealer 26; C534916:epoxy resin-based root canal sealer; C074417:Endo-Fill; D002126:Calcium Hydroxide; D001729:Bismuth