title: Baculovirus surface display of E(rns) envelope glycoprotein of classical swine fever virus.
abstract: Classical swine fever virus (CSFV) causes significant losses in the pig industry in many countries. E(rns) is an envelope glycoprotein of CSFV which is known to induce virus-neutralizing antibodies and protective immunity in the natural host. In this study, one recombinant baculoviruses BacSC-E(rns) expressing histidine-tagged E(rns) with the transmembrane domain (TM) and cytoplasmic domain (CTD) derived from baculovirus envelope protein gp64 was constructed and its immunizing efficacy was evaluated in a mouse model. After infection, E(rns) was expressed and anchored on the plasma membrane of Sf-9 cells, as demonstrated by Western-blot and confocal microscopy. Immunogold electron microscopy demonstrated that the E(rns) glycoprotein was successfully displayed on the baculoviral envelope. Vaccine tests in animals showed that BacSC-E(rns) elicited significantly higher E(rns) antibody titers in the immunized mouse models than the control group. This demonstrates that the BacSC-E(rns) vaccine can be used potentially against CSFV infections. This is the first report demonstrating the potential of E(rns)-pseudotyped baculovirus as a CSFV vaccine.
authors: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan.
authors: Xin-Gang
authors: XG
authors: Xu
authors: Ming-Tang
authors: MT
authors: Chiou
authors: Yan-Ming
authors: YM
authors: Zhang
authors: De-Wen
authors: DW
authors: Tong
authors: Jian-He
authors: JH
authors: Hu
authors: Miao-Tao
authors: MT
authors: Zhang
authors: Hung-Jen
authors: HJ
authors: Liu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D006691:Classical Swine Fever / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018411:Spodoptera; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; C466501:E(rns) protein, classic swine fever virus; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines