Persistent propagation of variant Creutzfeldt-Jakob disease agent in murine spleen stromal cell culture with features of mesenchymal stem cells.
The transmission of variant Creutzfeldt-Jakob disease (vCJD) through blood transfusions has created new concerns about the iatrogenic spread of transmissible spongiform encephalopathies (TSEs)/prion diseases through blood and plasma-derived products and has increased the need to develop efficient methods for detection of the agent in biologics. Here, we report the first successful generation of spleen-derived murine stromal cell cultures that persistently propagate two mouse-adapted isolates of human TSE agents, mouse-adapted vCJD, and Fukuoka 1. These new cell cultures can be used efficiently for studies of the pathogenesis of the disease, for development of diagnostics and therapeutics, and as a rapid ex vivo assay for TSE inactivation/removal procedures.
Akimov
Sergey
S
Transmissible Diseases Department, American Red Cross Holland Laboratory, Rockville, Maryland 20855, USA.
Yakovleva
Oksana
O
Vasilyeva
Irina
I
McKenzie
Carroll
C
Cervenakova
Larisa
L
D000818:Animals; D007562:Creutzfeldt-Jakob Syndrome / Q000175:diagnosis*; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells; D051379:Mice; D011328:Prions / Q000378:metabolism*; D013154:Spleen / Q000166:cytology*; D017154:Stromal Cells
D011328:Prions