In vitro culture under physiologic oxygen concentration improves blastocyst yield and quality: a prospective randomized survey on sibling oocytes.
A prospective randomized study was carried on sibling oocytes in which human gametes and embryos were cultured at physiologic (6% CO(2) + 5% O(2) + 89% N(2)) condition from insemination to day 6 or at atmospheric (6% CO(2) in air) condition until day 3 and at physiologic environment afterward. The results showed that physiologic oxygen concentration throughout culture period improved total blastocyst yield and day 5 embryo quality.
Ciray
H Nadir
HN
IVF Unit, German Hospital Istanbul, Istanbul, Turkey.
Aksoy
Turan
T
Yaramanci
Kursat
K
Karayaka
Ilyas
I
Bahceci
Mustafa
M
D001755:Blastocyst / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D046149:Embryo Culture Techniques / Q000379:methods*; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D008297:Male; D054315:Oocyte Retrieval; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D011446:Prospective Studies; D035781:Siblings; D020554:Sperm Injections, Intracytoplasmic
D010100:Oxygen