Proteins and peptidases from conidia and mycelia of Scedosporium apiospermum strain HLPB.
The conidia-mycelia transformation is an essential step during the life cycle of the fungal human pathogens of the Pseudallescheria boydii complex. In the present study, we have analyzed the protein and peptidase profiles in two distinct morphological stages, conidia and mycelia, of Scedosporium apiospermum sensu stricto. Proteins synthesized by the mycelia, migrating at the ranges of 62-48 and 22-18 kDa, were not detected from the conidial extract. Conidia produced a single cellular peptidase of 28 kDa able to digest copolymerized albumin, while mycelia yielded 6 distinct peptidases ranging from 90 to 28 kDa. All proteolytic enzymes were active at acidic pH and fully inhibited by 1,10-phenanthroline, characterizing these activities as metallo-type peptidases. Quantitative peptidase assay, using soluble albumin, showed a high metallopeptidase production in mycelial cells in comparison with conidia. The regulated expression of proteins and peptidases in different morphological stages of S. apiospermum represents a potential target for isolation of stage-specific markers for biochemical and immunological analysis.
Pereira
Martha Machado
MM
Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes (IMPPG), Centro de Ciências da Saúde (CCS), Bloco I, Universidade Federal do Rio de Janeiro (UFRJ), Ilha do Fundão, Rio de Janeiro, Brazil.
Silva
Bianca Alcântara
BA
Pinto
Marcia Ribeiro
MR
Barreto-Bergter
Eliana
E
dos Santos
André Luis Souza
AL
D000418:Albumins / Q000378:metabolism; D015415:Biomarkers; D005353:Fibronectins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D045726:Metalloproteases / Q000302:isolation & purification / Q000378:metabolism; D009077:Mucins / Q000378:metabolism; D025282:Mycelium / Q000201:enzymology / Q000235:genetics; D010618:Phenanthrolines / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D021681:Scedosporium / Q000201:enzymology / Q000235:genetics; D013172:Spores, Fungal / Q000201:enzymology / Q000235:genetics
D000418:Albumins; D015415:Biomarkers; D005353:Fibronectins; D006454:Hemoglobins; D007074:Immunoglobulin G; D007797:Laminin; D009077:Mucins; D010618:Phenanthrolines; D011480:Protease Inhibitors; D045726:Metalloproteases; C025205:1,10-phenanthroline