Loss of Drop1 expression already at early tumor stages in a wide range of human carcinomas.
In a study on gene deregulation in ovarian carcinoma we found a mRNA coding for a 350 kDa protein, Drop1, to be downregulated 20- to 180-fold in the majority of ovarian and mammary carcinomas. The mRNA is encoded by a set of exons in the 5' region of the SYNE1 gene. Immunohistochemical staining for Drop1 protein by a specific monoclonal antibody corresponds to the pattern seen for the mRNA. cDNA arrays of matched pairs of tumor and normal tissue and in situ hybridizations confirmed the drastic loss of Drop1 mRNA as a common feature in uterus, cervix, kidney, lung, thyroid and pancreas carcinomas, already at early tumor stages and in all metastases. Two-hybrid studies suggest a role of this deficiency in the malignant progression of epithelial tumors.
Marmé
Alexander
A
Department of Obstetrics and Gynecology, University of Heidelberg, Heidelberg, Germany.
Zimmermann
Hans-Peter
HP
Moldenhauer
Gerhard
G
Schorpp-Kistner
Marina
M
Müller
Claudia
C
Keberlein
Olga
O
Giersch
Antje
A
Kretschmer
Jürgen
J
Seib
Brigitte
B
Spiess
Eberhard
E
Hunziker
Andreas
A
Merchán
Faustino
F
Möller
Peter
P
Hahn
Uwe
U
Kurek
Raffael
R
Marmé
Frederik
F
Bastert
Gunther
G
Wallwiener
Diethelm
D
Ponstingl
Herwig
H
D001943:Breast Neoplasms / Q000235:genetics; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins; D005091:Exons; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009367:Neoplasm Staging; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020798:Two-Hybrid System Techniques
D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C415465:SYNE1 protein, human