Non-native glyceraldehyde-3-phosphate dehydrogenase can be an intrinsic component of amyloid structures.
Interactions between different forms of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and amyloid-beta peptide (1-42) were investigated by direct (surface plasmon resonance) and indirect (kinetics of spontaneous and GroEL/S-assisted reactivation of denatured GAPDH) methods. It was demonstrated that non-native forms of GAPDH obtained by different ways (cold denaturation, oxidation of the enzyme, and its unfolding in guanidine hydrochloride) efficiently bind to soluble amyloid-beta peptide (1-42) yielding a stable complex. Native tetrameric GAPDH does not interact with soluble amyloid-beta peptide (1-42), neither non-native forms of GAPDH interact with aggregated amyloid-beta peptide (1-42). The results suggest that non-native GAPDH species can be involved in the formation of amyloid structures during Alzheimer's disease, binding to soluble amyloid-beta peptide (1-42).
Naletova
Irina
I
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskie gory, 119992 Moscow, Russia.
Schmalhausen
Elena
E
Kharitonov
Aleksey
A
Katrukha
Aleksey
A
Saso
Luciano
L
Caprioli
Antonio
A
Muronetz
Vladimir
V
D000544:Alzheimer Disease / Q000201:enzymology*; D000682:Amyloid / Q000737:chemistry* / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D018833:Chaperonins / Q000737:chemistry; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011489:Protein Denaturation; D017510:Protein Folding; D011817:Rabbits; D020349:Surface Plasmon Resonance / Q000379:methods
D000682:Amyloid; D016229:Amyloid beta-Peptides; D001426:Bacterial Proteins; C097970:GroESL protein, Bacteria; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D018833:Chaperonins