Protein nanopatterns for improved immunodetection sensitivity.
In this work, we clearly demonstrate the capability of protein nanopatterns of improving the quality factors of immunosensing devices, such as lowering of the limit of detection and increase of sensitivity. This beneficial effect is obtained by the formation on the sensor's surface of bioadhesive domains of nanometric dimensions in a nonadhesive matrix by means of colloidal lithography.
Valsesia
A
A
European Commission, Joint Research Centre, Institute for Health and Consumer Protection, 21020 Ispra (VA), Italy. andrea.valsesia@jrc.it
Mannelli
I
I
Colpo
P
P
Bretagnol
F
F
Rossi
F
F
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011108:Polymers; D011506:Proteins; C048844:anti-IgG; D012710:Serum Albumin, Bovine; D010047:Ovalbumin