title: [Absorption mechanism of icariin across Caco-2 monolayer model].
abstract: OBJECTIVE
  To study the absorption mechanism of icariin by using Caco-2 monolayer model.
  
  
  METHODS
  Caco-2 cell monolayer model was used to study the bi-direction transport of icariin. The effects of time, drug concentration and inhibitor on the absorption of icariin were studied. The concentration of icariin in cell culture medium was measured by UPLC and the apparent permeability coefficients (Papp) was calculated.
  
  
  RESULTS
  The amount of icariin which was transported increased linearly with the time (14 hr). The ratio of PBA/PAB was larger than 4. Verapamil, the P-glycoprotein inhibitor, could cause significantly effect on transport of icariin, PAB increased, the ratio of PBA/PAB decreased.
  
  
  CONCLUSIONS
  The reason for low absorption of icariin in Caco-2 cell model may be the secretion of the P-gp transporter.
authors: Jiangsu Provincial Academy of Traditional Chinese Medicine, Jiangsu Engineering and Technology Research Center for Modern Chinese Pharmaceutical Preparation, Nanjing 210028, China. ychen202@yahoo.com.cn
authors: Yan
authors: Y
authors: Chen
authors: Xiao-Bin
authors: XB
authors: Jia
authors: Ming
authors: M
authors: Hu
authors: An-Wei
authors: AW
authors: Ding
mesh_terms: D000042:Absorption; D018938:Caco-2 Cells; D034181:Epimedium / Q000737:chemistry; D005419:Flavonoids / Q000493:pharmacokinetics*; D006801:Humans; D008954:Models, Biological
chemical_list: D005419:Flavonoids; C056599:icariin