title: Biomonitoring of genotoxicity using micronuclei assay in native population of Astyanax jacuhiensis (Characiformes: Characidae) at sites under petrochemical influence.
abstract: Bom Jardim brook is a small stream that flows through an area under the influence of a Petrochemical Complex, demanding control over its quality, so a genotoxic evaluation was performed. This study was conducted in situ, based on previous analysis on the same subject. These were performed both in vitro, with Salmonella typhimurium and human lymphocytes, and in vivo, using bioassays with fish exposed to water from the study area. The purpose of this research was to assess the quality of the aquatic environment and possible effects from petrochemical pollution to surrounding native populations. Micronuclei (MNE) and nuclear abnormalities (NA) frequencies in peripheral blood of Astyanax jacuhiensis, a native fish species collected from the study area, were used as biomarkers. Study period was from summer/99 to spring/2001, using samples obtained seasonally at two ponds upstream from the industrial area (BJN and BJPa) and two sites in Bom Jardim brook (BJ002 and BJ000), which are subject to Complex influence. MNE and NA frequencies found in individuals from BJ002 and BJ000 were similar, showing positive genotoxic responses related to control sites BJN and BJPa. No differential sensitivity could be verified for micronuclei induction between genders of A. jacuhiensis in the studied population. This study showed that sites subject to petrochemical influence were under higher genotoxic impact. Biomarkers adequacy to the case and the sensitivity of A. jacuhiensis for water monitoring could be also inferred.
authors: Divisão de Biologia, Programa de Pesquisas Ambientais, Departamento de Laboratórios, Fundação Estadual de Proteção Ambiental Henrique Luís Roessler (FEPAM), Avenida Dr. Salvador França, 1707, 90690-000, Porto Alegre, RS, Brazil. claricetl@fepam.rs.gov.br
authors: Clarice Torres
authors: CT
authors: de Lemos
authors: Fábio de Almeida
authors: Fde A
authors: Iranço
authors: Nânci Cristina D'Avila
authors: NC
authors: de Oliveira
authors: Getúlio Dornelles
authors: GD
authors: de Souza
authors: Jandyra Maria Guimarães
authors: JM
authors: Fachel
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D003530:Cyprinidae / Q000097:blood*; D004784:Environmental Monitoring; D006801:Humans; D007221:Industry; D008214:Lymphocytes; D015162:Micronucleus Tests / Q000379:methods*; D009152:Mutagenicity Tests; D010578:Petroleum / Q000633:toxicity*; D012486:Salmonella typhimurium; D012621:Seasons; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000097:blood / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D010578:Petroleum; D014874:Water Pollutants, Chemical