title: Molecular cloning, expression, and characterization of mouse amine N-sulfotransferases.
abstract: By searching the GenBank database, we recently identified a novel mouse cytosolic sulfotransferase (SULT) cDNA (IMAGE Clone ID 679629) and a novel mouse SULT gene (LOC 215895). Sequence analysis revealed that both mouse SULTs belong to the cytosolic SULT3 gene family. The recombinant form of these two newly identified SULTs, designated SULT3A1 and SULT3A2, were expressed using the pGEX-4T-1 glutathione S-transferase fusion system and purified from transformed BL21 Escherichia coli cells. Both purified SULT3A1 and SULT3A2 exhibited strong amine N-sulfonating activities toward 1-naphthylamine among a variety of endogenous and xenobiotic compounds tested as substrates. Kinetic constants of the sulfation of 1-naphthylamine and 1-naphthol by these two enzymes were determined. Collectively, these results imply that these two amine-sulfonating SULT3s may play essential roles in the metabolism and detoxification of aromatic amine compounds in the body.
authors: Department of Biochemistry and Applied Biosciences, University of Miyazaki, 1-1, Gakuenkibanadai-Nishi, Miyazaki, Miyazaki 889-2192, Japan.
authors: Saki
authors: S
authors: Takahashi
authors: Yoichi
authors: Y
authors: Sakakibara
authors: Emi
authors: E
authors: Mishiro
authors: Haruna
authors: H
authors: Kouriki
authors: Rika
authors: R
authors: Nobe
authors: Katsuhisa
authors: K
authors: Kurogi
authors: Shin
authors: S
authors: Yasuda
authors: Ming-Cheh
authors: MC
authors: Liu
authors: Masahito
authors: M
authors: Suiko
mesh_terms: D000588:Amines / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008099:Liver / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*
chemical_list: D000588:Amines; D011994:Recombinant Proteins; C532502:SULT3A1 protein, mouse; C532503:SULT3A2 protein, mouse; D015238:Sulfotransferases