title: Molecular detection of spotted fever group Rickettsia in Dermacentor silvarum from a forest area of northeastern China.
abstract: In total, 676 Dermacentor silvarum Olenev (Acari: Ixodidae) from a forest area of Jilin Province in northeastern China were examined by polymerase chain reaction for the presence of spotted fever group (SFG) Rickettsia. The overall positive rate was 10.7%, with a 95% confidence interval from 8.3 to 13.0%. The SFG Rickettsia infection was more prevalent in adults than in nymphs, and in fed ticks obtained from domestic animals than in those collected on vegetation. Sequence analysis of the partial outer membrane protein A gene confirmed the existence of R. sibirica and discovered a novel rickettsial agent in this area, the sequence of which was identical to that of DnS14 genotype Rickettsia previously reported in the former Soviet Union.
authors: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, 20 Dong-Da St., Fengtai District, Beijing 100071, People's Republic of China. caowc@nic.bmi.ac.cn
authors: Wu-Chun
authors: WC
authors: Cao
authors: Lin
authors: L
authors: Zhan
authors: Sake J
authors: SJ
authors: De Vlas
authors: Bo-Hai
authors: BH
authors: Wen
authors: Hong
authors: H
authors: Yang
authors: Jan H
authors: JH
authors: Richardus
authors: J Dik F
authors: JD
authors: Habbema
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D003870:Dermacentor / Q000145:classification / Q000235:genetics / Q000382:microbiology*; D006801:Humans; D012284:Rickettsia rickettsii / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D012373:Rocky Mountain Spotted Fever / Q000453:epidemiology / Q000635:transmission*
chemical_list: D004269:DNA, Bacterial; D004247:DNA