[Development of isolated swine "working heart model" with parabiotic circulation].
OBJECTIVE
To develop an isolated working heart model with parabiotic circulation in swine and verify its stability and possibility to allow effective measurements of hemodynamic and metabolic data.


METHODS
This model was developed during study of association of agents to cardiology. There were performed 18 experiments, each with a support animal and a donor animal. Donor animal heart was perfused as isolated working heart with parabiotic circulation from support animal. Isolated heart underwent regional ischemia by interventricular artery clamping, followed by global ischemia. During reperfusion in working heart state mode at 30, 60 and 90 minutes, contractility indices such as elastance, preload recruitable stroke work index and metabolic data were acquired.


RESULTS
Support animals were kept stable throughout the procedures without use of blood transfusions or vasoactive drugs. pH, oxygen partial pressure and hematocrit were kept stable and within physiologic ranges. Isolated heart was perfused adequately throughout the experiment. All hemodynamic and metabolic data proposed were adequately measured in working heart state mode.


CONCLUSIONS
This swine isolated "working heart" model was kept stable throughout the experiments with no administration of vasoactive drugs and it allowed adequate measurements of metabolic and hemodynamic data.
Silveira Filho
Lindemberg da Mota
Lda M
Faculdade de Ciências Médicas, Universidade Estadual de Campinas. lindembergms@uol.com.br
Petrucci
Orlando
O
Carmo
Marcio Roberto do
MR
Oliveira
Pedro Paulo Martins de
PP
Vilarinho
Karlos Alexandre de Sousa
KA
Vieira
Reinaldo Wilson
RW
Braile
Domingo Marcolino
DM
D000818:Animals; D001639:Bicarbonates / Q000008:administration & dosage; D002122:Calcium Chloride / Q000008:administration & dosage; D003427:Cross Circulation; D005260:Female; D006321:Heart / Q000502:physiology*; D006439:Hemodynamics; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000008:administration & dosage; D023421:Models, Animal; D009200:Myocardial Contraction / Q000502:physiology*; D015425:Myocardial Reperfusion / Q000379:methods*; D009206:Myocardium / Q000378:metabolism; D011189:Potassium Chloride / Q000008:administration & dosage; D012965:Sodium Chloride / Q000008:administration & dosage; D013552:Swine
D001639:Bicarbonates; C041711:St. Thomas' Hospital cardioplegic solution; D012965:Sodium Chloride; D011189:Potassium Chloride; D008274:Magnesium; D002122:Calcium Chloride