title: Radioimmunoassay for autoantibodies against interferon omega; its use in the diagnosis of autoimmune polyendocrine syndrome type I.
abstract: Patients with the autoimmune polyendocrine syndrome I (APS I) have high titers of neutralizing IgG autoantibodies against type I interferons (IFNs), in particular IFN-omega. Until now, the most specific assay has been the antiviral interferon neutralizing assay (AVINA), which has the drawbacks of requiring a cytolytic virus, being cumbersome and difficult to standardise. We have developed a fast and reliable immunoassay based on radiolabelled IFN-omega for quantifying anti-IFN-omega antibodies. Sera from 48 APS I patients were analysed together with those from 5 control groups. All sera from APS I patients were positive for anti-IFN-omega, while, except one serum, all sera from the controls were negative. This method has the advantage over bioassays that it is readily adapted to high throughput. It provides an alternative, sensitive and specific diagnostic test for APS I, and an ideal screening tool to precede mutational analyses of the AIRE gene in suspected APS I cases.
authors: Section of Endocrinology, Institute of Medicine, University of Bergen, Bergen, Norway. Bergithe.oftedal@med.uib.no
authors: Bergithe E
authors: BE
authors: Oftedal
authors: Anette S Bøe
authors: AS
authors: Wolff
authors: Eirik
authors: E
authors: Bratland
authors: Olle
authors: O
authors: Kämpe
authors: Jaakko
authors: J
authors: Perheentupa
authors: Anne Grethe
authors: AG
authors: Myhre
authors: Anthony
authors: A
authors: Meager
authors: Radhika
authors: R
authors: Purushothaman
authors: Svetlana
authors: S
authors: Ten
authors: Eystein S
authors: ES
authors: Husebye
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D005260:Female; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D008297:Male; D016884:Polyendocrinopathies, Autoimmune / Q000175:diagnosis* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D013577:Syndrome
chemical_list: D001323:Autoantibodies; D007370:Interferon Type I; C063743:interferon omega 1