Quantitative and qualitative analysis of proteins in fresh frozen plasma obtained from whole blood donations and prepared with two photochemical treatments.
Thirty-six fresh frozen plasma (FFP) units obtained from whole blood donations were used for 12 replicate experiments. For each replicate experiment, three ABO-matched FFP units were pooled and divided into three units containing different volumes of identical plasma. One unit was used as control FFP, one unit was treated with methylene blue plus visible light and one unit was treated with amotosalen and UVA light. The overall coagulation factor levels were better maintained in untreated FFP than in photochemically treated plasma. However, treated-plasma by both photochemical methods maintained coagulation factor levels that met or exceeded the European Pharmacopeia requirements for therapeutic plasma.
Cid
Joan
J
Banc de Sang i Teixits, Barcelona, Spain. jcid.hj23.ics@gencat.cat
Ramiro
Laia
L
Martínez
Nuria
N
Palomo
Marta
M
Magallon
Olga
O
Claparols
Montse
M
Ortiz
Pilar
P
Puig
Lluis
L
Pla
Ramon Pau
RP
D001779:Blood Coagulation Factors / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D017848:Blood-Borne Pathogens / Q000528:radiation effects; D005340:Fibrinogen / Q000032:analysis; D011564:Furocoumarins / Q000494:pharmacology / Q000528:radiation effects; D006801:Humans; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology* / Q000528:radiation effects; D010777:Photochemistry; D010949:Plasma / Q000737:chemistry* / Q000187:drug effects; D014466:Ultraviolet Rays; D038301:Virus Inactivation; D014841:von Willebrand Factor / Q000032:analysis
D001779:Blood Coagulation Factors; D001798:Blood Proteins; D011564:Furocoumarins; D014841:von Willebrand Factor; D005340:Fibrinogen; C118577:amotosalen; D008751:Methylene Blue