Identification of early-flower-related ESTs in an early-flowering mutant of trifoliate orange (Poncirus trifoliata) by suppression subtractive hybridization and macroarray analysis.
To increase our understanding of the genes involved in flower development in citrus, we identified genes differentially expressed between juveniles and adults of an early-flowering mutant of trifoliate orange (precocious trifoliate orange, Poncirus trifoliata L. Raf.) by suppressive subtractive hybridization (SSH) and macroarray hybridization analyses. Based on dot blot analysis, we confirmed that the juvenile subtracted cDNA library comprised genes upregulated during floral induction, inflorescence development and flowering. A total of 190 nonredundant expressed sequence tags were identified that were related flower development. The temporal and spatial expression patterns of four SSH-enriched genes were studied in adult precocious trifoliate orange by real-time PCR. Among these differentially expressed genes, the expression pattern of C5-B16 was closely correlated with inflorescence development and flowering. The full-length cDNA of C5-B16 was isolated by 5' and 3' rapid amplification of cDNA ends. Sequence analysis indicated that C5-B16 is a novel gene in citrus.
Zhang
Jin-Zhi
JZ
Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, P.R. China.
Li
Zhi-Min
ZM
Liu
Li
L
Mei
Li
L
Yao
Jia-Ling
JL
Hu
Chun-Gen
CG
D003001:Cloning, Molecular; D019295:Computational Biology; D020224:Expressed Sequence Tags; D044783:F-Box Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015723:Gene Library; D018519:Meristem / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D018520:Plant Shoots / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D016133:Polymerase Chain Reaction; D032104:Poncirus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017422:Sequence Analysis, DNA
D044783:F-Box Proteins; D010940:Plant Proteins