title: Up-regulation of E-cadherin by small activating RNA inhibits cell invasion and migration in 5637 human bladder cancer cells.
abstract: Recent studies have reported that chemically synthesized small duplex RNAs complementary to promoters of target genes can specifically induce gene expression in several cancer cell lines. Such dsRNA, referred to as small activating RNA (saRNA), are involved in the recently described phenomenon called RNA activation (RNAa). Recent findings show that saRNA can inhibit cell proliferation and viability via up-regulation of p21(WAF1/CIP1) (p21) in human bladder cancer cells. In the present study, we demonstrate that induction of E-cadherin expression by saRNA leads to suppression of migration and invasion of 5637 human bladder cancer cells in vitro. The elevated E-cadherin expression was confirmed at transcriptional and protein levels after transfection of a 21-nucleotide dsRNA targeting the E-cadherin promoter (dsEcad). Furthermore, this inhibitory effect was associated with relocalization of beta-catenin from the nucleus to the plasma membrane and decreased beta-catenin-mediated transactivation. These data suggest that activation of E-cadherin by saRNA may have a therapeutic benefit for bladder and other types of cancer.
authors: Department of Urology, First Affiliated Hospital, School of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province, China.
authors: Qiqi
authors: Q
authors: Mao
authors: Yubing
authors: Y
authors: Li
authors: Xiangyi
authors: X
authors: Zheng
authors: Kai
authors: K
authors: Yang
authors: Huafeng
authors: H
authors: Shen
authors: Jie
authors: J
authors: Qin
authors: Yu
authors: Y
authors: Bai
authors: Debo
authors: D
authors: Kong
authors: Xiaolong
authors: X
authors: Jia
authors: Liping
authors: L
authors: Xie
mesh_terms: D015820:Cadherins / Q000819:agonists* / Q000235:genetics; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D009361:Neoplasm Invasiveness; D021381:Protein Transport; D012330:RNA, Double-Stranded / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use*; D015533:Transcriptional Activation; D014162:Transfection; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D012330:RNA, Double-Stranded; D051176:beta Catenin