Serum autotaxin measurement in haematological malignancies: a promising marker for follicular lymphoma.
Autotaxin (ATX) is a tumour cell motility-stimulating factor originally isolated from melanoma cell supernatants. ATX is identical to lysophospholipase D, which produces a bioactive lipid mediator, lysophosphatidic acid (LPA), from lysophosphatidylcholine. ATX is overexpressed in various malignancies, including Hodgkin lymphoma, and ATX may stimulate tumour progression via LPA production. The present study measured the serum ATX antigen levels in patients with haematological malignancies using a recently developed automated enzyme immunoassay. The serum ATX antigen levels in patients with B-cell neoplasms, especially follicular lymphoma (FL), were higher than those in healthy subjects. Serum ATX antigen levels in FL patients were associated with tumour burden and changed in parallel with the patients' clinical courses. The serum ATX antigen levels were little affected by inflammation, unlike the soluble interleukin-2 receptor and beta2-microglobulin levels. As expected, the plasma LPA levels in FL patients were correlated with the serum ATX antigen levels. Given that leukaemic tumour cells from FL patients expressed ATX, the shedding of ATX from lymphoma cells probably leads to the elevation of serum ATX antigen levels. Our results suggest that the serum ATX antigen level may be a promising and novel marker for FL.
Masuda
Akiko
A
Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Nakamura
Kazuhiro
K
Izutsu
Koji
K
Igarashi
Koji
K
Ohkawa
Ryunosuke
R
Jona
Masahiro
M
Higashi
Katsumi
K
Yokota
Hiromitsu
H
Okudaira
Shinichi
S
Kishimoto
Tatsuya
T
Watanabe
Takuro
T
Koike
Yukako
Y
Ikeda
Hitoshi
H
Kozai
Yasushi
Y
Kurokawa
Mineo
M
Aoki
Junken
J
Yatomi
Yutaka
Y
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D008224:Lymphoma, Follicular / Q000097:blood*; D008245:Lysophospholipase / Q000097:blood; D008297:Male; D008875:Middle Aged; D009097:Multienzyme Complexes / Q000032:analysis / Q000097:blood*; D043264:Phosphodiesterase I / Q000032:analysis / Q000097:blood*; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases / Q000032:analysis / Q000097:blood*; D018709:Statistics, Nonparametric; D000099189:Lysophospholipase D
D014408:Biomarkers, Tumor; D008245:Lysophospholipase; D009097:Multienzyme Complexes; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D000099189:Lysophospholipase D