title: Simultaneous determination of potassium clavulanate and cefixime in synthetic mixtures by high-performance liquid chromatography.
abstract: A simple, precise, and sensitive high-performance liquid chromatographic method was developed and validated for the simultaneous determination of potassium clavulanate and cefixime in synthetic mixture form. The analytes were separated on a C18 column by using 0.03 M disodium hydrogen phosphate buffer (pH 6.5)-methanol (84 + 16, v/v) as the mobile phase with detection at 220 nm. The method exhibited high sensitivity and good linearity in the concentration ranges of 12.5-62.5 and 20-100 microg/mL for potassium clavulanate and cefixime, respectively. The total run time for the 2 components was <8 min, and the average recovery was >101.5% with a relative standard deviation of <1.0%. The proposed method was validated according to guidelines of the International Conference on Harmonization by evaluation of linearity, recovery, selectivity, robustness, limits of detection and quantitation, and within- and between-day precision. The results obtained for the synthetic mixture show that the method is highly precise and accurate for the simultaneous determination of potassium clavulanate and cefixime.
authors: Government College University, Faculty of Science, Department of Chemistry, Lahore-54000, Pakistan.
authors: Islam Ullah
authors: IU
authors: Khan
authors: Shahzad
authors: S
authors: Sharif
authors: Muhammad
authors: M
authors: Ashfaq
authors: Muhammad Nadeem
authors: MN
authors: Asghar
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D020682:Cefixime / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D019818:Clavulanic Acid / Q000032:analysis*; D004338:Drug Combinations; D004355:Drug Stability; D007202:Indicators and Reagents; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions
chemical_list: D000900:Anti-Bacterial Agents; D004338:Drug Combinations; D007202:Indicators and Reagents; D012996:Solutions; D019818:Clavulanic Acid; D020682:Cefixime