title: [Isolation and characterization of a new glyphosate-resistant strain from extremely polluted environment].
abstract: OBJECTIVE
  To isolate and characterize a glyphosate-resistant strain from extremely polluted environment.
  
  
  METHODS
  A glyphosate-resistant strain was isolated from extremely polluted soil taking glyphosate as the selection pressure. Its glyphosate resistance, growth optimal pH and antibiotic sensitivity were detected. Its morphology, cultural characteristics, physiological and biochemical properties, chemotaxonomy and 16S rDNA sequences were studied. Based on these results, the strain was identified according to the ninth edition of Bergey's manual of determinative bacteriology.
  
  
  RESULTS
  The isolate was named SL06500. It could grow in M9 minimal medium containing up to 500 mmol/L glyphosate. The cell growth optimal pH of SL06500 was 4.0. It was resistant to ampicillin, kanamycin, tetracycline and chloromycetin. The 16S rDNA of SL06500 was amplified by PCR and sequenced. Compared with the published nucleotide sequence of 16S rDNA in NCBI (National Center for Biotechnology Information), SL06500 showed high identity with Achromobacter and Alcaligenes. Based on morphological, physiological and biochemical characteristics, the strain was identified as Alcaligenes xylosoxidans subsp.xylosoxidans SL06500 according to the ninth edition of Bergey's manual of determinative bacteriology.
  
  
  CONCLUSIONS
  Strain SL06500 is worthy to be studied because of its high glyphosate resistance.
authors: College of Life Sciences, Sichuan Normal University, Chengdu 610068, China. shajiying@yahoo.com.cn
authors: Jiying
authors: J
authors: Sh
authors: Dan
authors: D
authors: Jin
authors: Wei
authors: W
authors: Lu
authors: Xiaoyu
authors: X
authors: Zhang
authors: Chao
authors: C
authors: Zhang
authors: Liang
authors: L
authors: Li
authors: Ruiqiang
authors: R
authors: Ma
authors: Lei
authors: L
authors: Xiao
authors: Yiding
authors: Y
authors: Wang
authors: Min
authors: M
authors: Lin
mesh_terms: D000421:Alcaligenes / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004275:DNA, Ribosomal / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D004785:Environmental Pollutants / Q000494:pharmacology*; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D000097797:Glyphosate
chemical_list: D000900:Anti-Bacterial Agents; D004275:DNA, Ribosomal; D004785:Environmental Pollutants; D005998:Glycine; D006540:Herbicides; D000097797:Glyphosate