title: Clinical significance of micrometastases detection in lymph nodes from head and neck squamous cell carcinoma.
abstract: OBJECTIVE
  Study the clinical relevance of micrometastases in head and neck squamous cell carcinoma (HNSCC).
  
  
  METHODS
  We reviewed the outcome of 31 patients who underwent neck dissection for HNSCC with lymph node analysis by cytokeratin 19 real-time Taqman polymerase chain reaction (CK19RT-PCR) for detection of micrometastasis. Fifteen patients were N+ on histopathology (group 1) and 16 were N-; nine of these 16 patients were CK19RT-PCR positive (group 2), whereas seven were negative (group 3). Local and neck recurrences, metastases, and other tumour sites were recorded during follow-up.
  
  
  RESULTS
  Five patients in group 1, eight patients in group 2, and one patient in group 3 experienced a tumor-related event. N- patients in groups 2 and 3 had a different outcome (P<0.01).
  
  
  CONCLUSIONS
  It is suggested that CK19RTPCR detection of micrometastasis in lymph nodes could be of significant prognostic value in HNSCC, because more aggressive treatment could be indicated in these patients.
authors: Service d'ORL et Chirurgie Cervico-Faciale, Hôpital Tenon, Paris, France.
authors: Yanan
authors: Y
authors: Xu
authors: Marine
authors: M
authors: Lefèvre
authors: Sophie
authors: S
authors: Périé
authors: Lei
authors: L
authors: Tao
authors: Patrice
authors: P
authors: Callard
authors: Jean-François
authors: JF
authors: Bernaudin
authors: Jean Lacau
authors: JL
authors: St Guily
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D053539:Keratin-19 / Q000378:metabolism; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies
chemical_list: D053539:Keratin-19