Flavonoid glycosides isolated from Salicornia herbacea inhibit matrix metalloproteinase in HT1080 cells.
Flavonoid glycosides, isorhamnetin 3-capital O, Cyrillic-beta-d-glucoside, and quercetin 3-O-beta-d-glucoside were isolated from Salicornia herbacea and their inhibitory effects on matrix metalloproteinase-9 and -2 (MMP-9 and -2) were evaluated in human fibrosarcoma cell line (HT1080). In zymography experiments, these flavonoid glycosides led to the reduction of the expression levels and activities of MMP-9 and -2 without any significant difference between these flavonoid glycosides. Protein expression levels of both MMP-9 and MMP-2 were inhibited and TIMP-1 (tissue inhibitor of metalloproteinase-1) protein level was enhanced by these flavonoid glycosides. Moreover, a transfection study carried out with AP-1 reporter construct revealed that the reporter activity was suppressed by treatment with isorhamnetin 3-capital O, Cyrillic-beta-d-glucoside. Therefore, these results suggested that these flavonoid glycosides have a potential as valuable natural chemopreventive agents for cancer.
Kong
Chang-Suk
CS
Research Institute of Marine Science and Technology, Korea Maritime University, Busan 606-791, Republic of Korea.
Kim
You Ah
YA
Kim
Moon-Moo
MM
Park
Jin-Sook
JS
Kim
Jung-Ae
JA
Kim
Se-Kwon
SK
Lee
Burm-Jong
BJ
Nam
Taek Jeong
TJ
Seo
Youngwan
Y
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019663:Chenopodiaceae / Q000737:chemistry*; D005354:Fibrosarcoma / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D044948:Flavonols / Q000302:isolation & purification / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000187:drug effects / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014162:Transfection
D000970:Antineoplastic Agents; D005419:Flavonoids; D044948:Flavonols; D006027:Glycosides; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D019715:Tissue Inhibitor of Metalloproteinase-1; D018808:Transcription Factor AP-1; C016527:isoquercitrin; D011794:Quercetin; C432511:isorhamnetin 3-O-glucoside; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9