Intrathecal upregulation of IFN-gamma and MIP-1beta in juvenile muscular atrophy of the distal upper extremity.
Juvenile muscular atrophy of the distal upper extremity (JMADUE) is associated with airway allergy and hyperIgEaemia, suggesting the involvement of immunological processes. In this study we aimed to clarify the changes in various cytokines/chemokines in cerebrospinal fluid (CSF) from JMADUE patients. We simultaneously measured 17 cytokines/chemokines in sera and CSF from 6 patients with JMADUE before treatment and from 14 patients with cervical spondylosis (CS) as a disease control (mean age at examination 23+/-7 and. 57+/-16 years, respectively), using a fluorescent bead-based immunoassay. We also assayed CSF from a JMADUE patient before and after plasma exchanges. In sera, only an increase of MIP-1beta (CCL3) in the JMADUE patients had a marginal significance as compared with the CS patients. In the CSF, IFN-gamma and MIP-1beta (CCL3) were significantly elevated in JMADUE patients compared with controls (1.5 and 2-fold increases, respectively), while no other cytokines/chemokines showed any significant differences. Moreover, the upregulated cytokines decreased after plasma exchanges in accord with improvement of distal upper limb weakness. The intrathecal upregulation of proinflammatory Th1 cytokines/chemokines, such as IFN-gamma and MIP-1beta (CCL3), in the CSF of JMADUE patients indicates the possible involvement of intrathecal immunological processes in this condition.
Tanaka
Masahito
M
Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan.
Ishizu
Takaaki
T
Ochi
Hirofumi
H
Kawano
Yuji
Y
Ohyagi
Yasumasa
Y
Kira
Jun-ichi
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D054407:Chemokine CCL4 / Q000134:cerebrospinal fluid*; D016207:Cytokines / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D053580:Muscle Strength / Q000502:physiology; D009133:Muscular Atrophy / Q000134:cerebrospinal fluid* / Q000473:pathology* / Q000628:therapy; D010951:Plasma Exchange / Q000379:methods; D055009:Spondylosis / Q000134:cerebrospinal fluid / Q000473:pathology / Q000628:therapy; D018709:Statistics, Nonparametric; D034941:Upper Extremity / Q000503:physiopathology*; D055815:Young Adult
D054407:Chemokine CCL4; D016207:Cytokines; D007073:Immunoglobulin E; D007371:Interferon-gamma