Analysis of anti-platelet aggregation components of Rhizoma Zingiberis using chicken thrombocyte extract and high performance liquid chromatography.
BACKGROUND
The conventional procedure for screening bioactive components from traditional Chinese medicine is time-consuming, expensive and low efficient. Therefore, some alternative strategies are needed urgently. A novel method for screening anti-platelet aggregation components from oleoresins was developed using chicken thrombocyte extract and high performance liquid chromatography.


METHODS
The anti-platelet aggregation components of oleoresins were combined with receptors, channels and enzymes of chicken thrombocytes under physiological environment. Unbound substances were washed away and bound compounds were eluted using specific phosphate buffered solution (PBS). Compounds released from target sites were collected and analyzed by high performance liquid chromatography and LC-MS. The activity of three compounds which were screened from this model was confirmed using platelet aggregation pharmacology in vivo.


RESULTS
There were four typical compounds that bound to the thrombocytes: 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol, and all had shown anti-platelet aggregation activities. Eight-gingerol displayed the best anti-platelet aggregation effect.


CONCLUSIONS
Chicken thrombocyte extract can be used to isolate chemicals that are ligands of the receptor or other bio-targets on the platelet. This may therefore be a simple and efficient method to screen for anti-platelet aggregation compounds from traditional Chinese medicine.
Nie
Hong
H
Jinan University College of Pharmacy, Guangzhou, Guangdong, China. tnieh@jnu.edu.cn
Meng
Lan-zhen
LZ
Zhang
Hui
H
Zhang
Jian-yu
JY
Yin
Zhen
Z
Huang
Xue-song
XS
D000818:Animals; D002396:Catechols / Q000302:isolation & purification / Q000494:pharmacology; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005233:Fatty Alcohols / Q000302:isolation & purification / Q000494:pharmacology; D020939:Zingiber officinale / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry; D013601:T-Lymphocytes / Q000378:metabolism
D002396:Catechols; D005233:Fatty Alcohols; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; C018620:oleoresins; C007845:gingerol