Purification of pediocin PA-1 by immunoaffinity chromatography.
A one-step purification procedure for purifying pediocin PA-1, a class IIa bacteriocin produced by Pediococcus acidilactici UL5, was developed based on column immunoaffinity chromatography with specific antipediocin PA-1 polyclonal antibodies coupled to cyanogen bromide-activated SepharoseTM. About 13.3 microg/mL purified pediocin PA-1 was obtained from 15 mL P. acidilactici UL5 culture supernatant, as measured by enzyme-linked immunosorbent assay. The specific activity and average recovery of the eluted pediocin PA-1 were about 6602 AU/mg and 53.3%, respectively. This is the first report of successful purification of pediocin PA-1 by immunoaffinity using pediocin PA-1-specific polyclonal antibodies.
Naghmouchi
Karim
K
Agriculture and Agri-Food Canada, Lethbridge Research Center, Lethbridge, AB, Canada. naghmouchik@agr.gc.ca
Drider
Djamel
D
Fliss
Ismail
I
D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000918:Antibody Specificity; D001419:Bacteria / Q000187:drug effects; D001430:Bacteriocins / Q000302:isolation & purification* / Q000494:pharmacology; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006863:Hydrogen-Ion Concentration; D007120:Immunochemistry; D008826:Microbial Sensitivity Tests; D000070017:Pediocins; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D000070017:Pediocins; C074288:pediocin PA-1