In vitro cytotoxicity of maxillofacial silicone elastomers: effect of accelerated aging.
The purpose of this in vitro study was to evaluate the cytotoxicity of three maxillofacial silicone elastomers at 24, 48, and 72 h on L-929 cells and to determine the effect of accelerated aging on the cytotoxicity of these silicone elastomers. Disc-shaped test samples of maxillofacial silicone elastomers (Cosmesil, Episil, Multisil) were fabricated according to manufacturers' instructions under aseptic conditions. Samples were then divided into three groups: (1) not aged; (2) aged for 150 h with an accelerated weathering tester; and (3) aged for 300 h. Then the samples were placed in Dulbecco's Modified Eagle Medium/Ham's F12 (DMEM/F12) for 24, 48, and 72 h. After the incubation periods, cytotoxicity of the extracts to cultured fibroblasts (L-929) was measured by MTT assay. The degree of cytotoxicity of each sample was determined according to the reference value represented by the cells with a control (culture without sample). Statistical significance was determined by repeated measurement ANOVA (p < 0.01) followed by Duncan's test (p < 0.05). All test materials in each group demonstrated high survival rates in MTT assay (Episil; 93.84%, Multisil; 88.30%, Cosmesil; 87.50%, respectively); however, in all groups, Episil material demonstrated significantly higher cell survival rate after each of the experimental incubation periods (p < 0.05). Accelerated aging for 150 and 300 h had no significant effect on the biocompatibility of maxillofacial silicone elastomers tested (p > 0.05).
Bal
Bilge Turhan
BT
Department of Prosthodontics, Faculty of Dentistry, Gazi University, Ankara, Turkey. bilgeturhan@gmail.com
Yilmaz
Handan
H
Aydin
Cemal
C
Karakoca
Seçil
S
Yilmaz
Sükran
S
D000375:Aging; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D008447:Maxillofacial Prosthesis; D051379:Mice; D012826:Silicone Elastomers / Q000737:chemistry / Q000494:pharmacology*
D001672:Biocompatible Materials; D012826:Silicone Elastomers; C545983:multisil