Pressurized liquid extraction of mate tea leaves.
The objective of this work is to investigate the influence of process parameters on the pressurized liquid extraction (PLE) of Ilex paraguariensis leaves. A factorial 2(6-2) experimental design was employed using responses as the extraction yield and the chromatographic profile of the extracts. The extraction time, polarity of solvent, amount of sample, numbers of PLE cycles, flushing volume and extraction temperature were selected as independent variables (factors). Results obtained indicated that the solvent polarity was the most significant variable in the study, while the amount of sample and extraction temperature also showed significant effect. The other variables did not present significant influence in the yield of extraction. GC/MS analysis of the extract enabled the identification of saturated hydrocarbons, fatty acids, fatty acid methyl esters, phytosterols and theobromine in the extracts. Quantitative analysis of four compounds presented in the extracts (caffeine, phytol, vitamin E and squalene) was performed by the GC/MS in the SIM mode.
Jacques
Rosângela Assis
RA
Universidade Federal do Pampa (UNIPAMPA), Rua Carlos Barbosa s/n degrees , Bagé 96412-420, RS, Brazil. rjacques@unipampa.ufpel.tche.br
Dariva
Cláudio
C
de Oliveira
José Vladimir
JV
Caramão
Elina Bastos
EB
D001628:Beverages / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D030019:Ilex paraguariensis / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D011312:Pressure; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D010936:Plant Extracts; D012997:Solvents