Comparison of one-day versus one-hour application of topical gatifloxacin in eliminating conjunctival bacterial flora.
OBJECTIVE
To compare efficacies of 1-day, 1-hour, and combined 1-day/1-hour preoperative topical gatifloxacin in eliminating conjunctival bacterial flora.


METHODS
Prospective, comparative case series.


METHODS
Sixty patients (120 eyes) scheduled to undergo anterior segment intraocular surgery at Stanford University Medical Center.


METHODS
Cultures were collected from the palpebral conjunctival sac at baseline and after 1 day (4 doses), 1 hour (3 doses), and 1 day/1 hour (7 doses) of gatifloxacin use.


METHODS
Incidence of positive bacterial samples collected pre- and post-antibiotic treatment and number of colony forming units (CFUs).


RESULTS
SeptiChek (Becton Dickinson, Franklin Lakes, NJ) positive cultures significantly decreased from 67% growth at baseline to 28% (P<0.0001) after 1 day and from 60% at baseline to 37% (P = 0.018) after 1 hour of gatifloxacin use. Reductions of 44% growth at baseline to 12% (P = 0.0001) after 1 day and 32% at baseline to 13% (P = 0.029) after 1 hour of gatifloxacin use were observed on blood agar. Surgical eyes that received both 1-day and 1-hour preoperative gatifloxacin had reductions from 67% growth at baseline to 18% posttreatment (P<0.0001) and 45% at baseline to 7% posttreatment (P<0.0001) on SeptiChek and blood agar media, respectively. In addition to a lower frequency of positive cultures, a significantly lower CFU count was found after 1-day (P = 0.004) and 1-hour (P = 0.049) gatifloxacin use compared with pretreatment levels. Combined 1-day/1-hour doses of gatifloxacin were associated with a greater reduction in CFUs (P = 0.001) when compared with 1-hour treatment alone.


CONCLUSIONS
Both 1-hour and 1-day topical gatifloxacin use are effective in reducing the frequency of conjunctival bacterial growth and the overall bacterial load as measured by CFUs, relative to baseline. Although a 1-hour pretreatment is associated with a reduction in bacterial growth, the combination of 1-day and 1-hour preoperative gatifloxacin dosing results in an even lower overall bacterial load, suggesting that the latter might be the preferred preoperative regimen for eyes undergoing anterior segment surgery.
Moss
Jason M
JM
Stanford University Medical Center, Department of Ophthalmology, Stanford, California, USA.
Nguyen
David
D
Liu
Yueyi I
YI
Singh
Kuldev
K
Montague
Artis
A
Egbert
Peter R
PR
Kaspar
Herminia Mino De
HM
Ta
Christopher N
CN
D000287:Administration, Topical; D000368:Aged; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D001431:Bacteriological Techniques; D002387:Cataract Extraction; D015169:Colony Count, Microbial; D003228:Conjunctiva / Q000382:microbiology*; D005260:Female; D024841:Fluoroquinolones / Q000008:administration & dosage*; D000077734:Gatifloxacin; D006801:Humans; D015948:Keratoplasty, Penetrating; D008297:Male; D011446:Prospective Studies; D013997:Time Factors
D000891:Anti-Infective Agents, Local; D024841:Fluoroquinolones; D000077734:Gatifloxacin