Distributions of the growth rate of the germ tubes and germination time of Penicillium chrysogenum conidia depend on water activity.
The effects of water activities for sporulation (a(wsp)) and germination (a(wge)) on the distributions of the growth rate of the germ tubes (mu) and the germination time (t(G)) of Penicillium chrysogenum conidia were determined by monitoring the length of the same germ tubes throughout the experiments automatically. No relationship between the individual t(G)'s and mu's could be established. Irrespective of the water activity for germination, mu was greater and t(G) was less for conidia produced at 0.95a(wsp) than that at 0.99a(wsp). At 0.99 a(wge) the mean and the standard deviation of t(G) were smaller than those obtained at 0.95a(wge). At 0.99a(wge), normal distributions for mu and t(G) were exhibited, but not at 0.95a(wge). The cumulative frequencies were used to reconstruct the germination curves. Great differences in the percentage of spores capable of germination (P(G)) and in the mean germination times between conidia produced at 0.95a(wsp) and at 0.99a(wsp) were clearly exhibited at 0.95a(wge), thus demonstrating the paramount influence of sporulation conditions on germination kinetics.
Judet
Daniela
D
Laboratoire de Génie des Procédés Microbiologiques et Alimentaires, Université de Bourgogne, ENS.BANA, 1 Esplanade Erasme, 21000 Dijon, France.
Bensoussan
Maurice
M
Perrier-Cornet
Jean-Marie
JM
Dantigny
Philippe
P
D015169:Colony Count, Microbial; D005516:Food Microbiology; D007700:Kinetics; D008954:Models, Biological; D009172:Mycology / Q000379:methods; D010408:Penicillium chrysogenum / Q000254:growth & development* / Q000502:physiology*; D013172:Spores, Fungal / Q000254:growth & development; D014867:Water / Q000378:metabolism*
D014867:Water