Tityus serrulatus venom peptidomics: assessing venom peptide diversity.
MALDI-TOF-TOF and de novo sequencing were employed to assess the Tityus serrulatus venom peptide diversity. Previous works has shown the cornucopia of molecular masses, ranging from 800 to 3000Da, present in the venom from this and other scorpions species. This work reports the identification/sequencing of several of these peptides. The majority of the peptides found were fragments of larger venom toxins. For instance, 28 peptides could be identified as fragments from Pape proteins, 10 peptides corresponded to N-terminal fragments of the TsK beta (scorpine-like) toxin and fragments of potassium channel toxins (other than the k-beta) were sequenced as well. N-terminal fragments from the T. serrulatus hypotensins-I and II and a novel hypotensin-like peptide could also be found. This work also reports the sequencing of novel peptides without sequence similarities to other known molecules.
Rates
Breno
B
Laboratório de Venenos e Toxinas Animais, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, Bloco O4, Sala 125, 6627, 31270-901, Belo Horizonte, Minas Gerais, Brasil.
Ferraz
Karla K F
KK
Borges
Márcia H
MH
Richardson
Michael
M
De Lima
Maria Elena
ME
Pimenta
Adriano M C
AM
D000595:Amino Acid Sequence; D000818:Animals; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D012604:Scorpion Venoms / Q000737:chemistry*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D010455:Peptides; D012604:Scorpion Venoms