Effect of constant glucose feeding on the production of exopolysaccharides by Tremella fuciformis spores.
A fed-batch culture system with constant feeding (glucose 80 g L(-1), 0.25 ml min(-1)) was used to study the influence of glucose on cell dry weight and exopolysaccharides production from submerged Tremella fuciformis spores in a 5-L stirred-tank bioreactor. The results showed that high levels of cell mass (9.80 g L(-1)) and exopolysaccharides production (3.12 g L(-1)) in fed-batch fermentation were obtained after 1 h of feeding, where the specific growth rate (mu) and exopolysaccharides yield on substrate consumed (YP/S) were 0.267 d(-1) and 0.14 g g(-1). Unlike batch fermentation, maximal cell mass and exopolysaccharides production merely reached 7.11 and 2.08 g L(-1); the specific growth rate (mu) and exopolysaccharides yield on substrate consumed (YP/S) were 0.194 d(-1) and 0.093 g g(-1), respectively. It is concluded that the synthesis of exopolysaccharides can be promoted effectively when feeding glucose at a late exponential phase.
Zhu
H
H
Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266555, People's Republic of China. hzhu@live.cn
Sun
S J
SJ
D001487:Basidiomycota / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D018533:Biomass; D005947:Glucose / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis*; D013172:Spores, Fungal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013997:Time Factors
D011134:Polysaccharides; D005947:Glucose; D010100:Oxygen