title: Preparation of an open-tubular capillary column with a monolithic layer of S-ketoprofen imprinted and 4-styrenesulfonic acid incorporated polymer and its enhanced chiral separation performance in capillary electrochromatography.
abstract: Enhanced chiral separation performance has been observed for ketoprofen enantiomers in capillary electrochromatography (CEC) with an open-tubular (OT) column prepared with a specific molecule imprinted polymer (MIP) on the innerwall of 50mum ID capillary. The column was prepared by in situ thermal polymerization inside the pretreated and silanized fused silica capillary. A specific diluted monomer mixture composed of S-ketoprofen, methacrylic acid (MAA, functional monomer), ethylene glycol dimethacrylate (EDMA, cross-linker), and 4-styrenesulfonic acid (4-SSA) dissolved in 9/1 (v/v) acetonitrile/2-propanol was used to fabricate the OT-MIP layer. 4-SSA was added to form a MIP layer capable of stable and strong electro-osmotic flow (EOF) over the pH range of this study securing CEC elution of ketoprofen having partial negative charge near the optimized pH. Various parameters such as buffer pH, organic modifier composition, salt concentration, and applied potential have been optimized for CEC chiral separation of ketoprofen enantiomers. Very good separation selectivity and efficiency were observed, thus the chromatographic resolution of ketoprofen enantiomers was as high as 10.5, and the number of theoretical plates of R-ketoprofen, 156,000/m (40,000/m for S-ketoprofen), which proves that the OT-MIP-CEC type approach is a promising strategy in MIP study.
authors: Department of Chemistry, Nano Fine Center, Institute of Basic Research, Inha University, 253 Yonghyung-Dong, Nam-ku, Incheon 402-751, South Korea.
authors: Shabi Abbas
authors: SA
authors: Zaidi
authors: Won Jo
authors: WJ
authors: Cheong
mesh_terms: D053801:Capillary Electrochromatography / Q000379:methods*; D007660:Ketoprofen / Q000302:isolation & purification*; D008689:Methacrylates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D011108:Polymers; D013451:Sulfonic Acids; D007660:Ketoprofen; C077114:styrenesulfonic acid polymer