The use of inductively coupled plasma mass spectrometry as a detector in drug metabolism studies.
BACKGROUND
The inherent properties of element selectivity combined with high sensitivity and structure independent response, make inductively coupled plasma mass spectrometry (ICP-MS) an interesting alternative detection technique in drug metabolism studies.


OBJECTIVE
The application of online separation with ICP-MS detection in drug metabolism studies is reviewed with focus on the merits and demerits of this detection technique. The prerequisite for inclusion in this review is that the study involves a separation technique hyphenated online to the ICP-MS detection.


CONCLUSIONS
ICP-MS detection is found to be advantageous for analysis of all drug substances detectable by ICP-MS compared to radiochemical detection. Detectable drugs are limited to halogen-, sulfur-, metal- and metalloid-containing compounds. The drawback of interference from endogenous compounds on quantitative mass balance estimations of non-metal drugs is addressed. The potential of determining the stoichiometry in metallo-drug biomolecule interactions is pointed out by presenting examples of simultaneous monitoring of metals in metallo-drugs and intrinsic ICP-MS detectable elements in biomolecules. It is concluded that ICP-MS detection is an indispensable technique in drug metabolism studies of metallo-drugs, although the applicability for traditional drugs is limited.
Gammelgaard
Bente
B
Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark. bg@farma.ku.dk
Hansen
Helle Rüsz
HR
Stürup
Stefan
S
Møller
Charlotte
C
D000818:Animals; D015195:Drug Design; D006219:Halogens / Q000032:analysis / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008670:Metals / Q000032:analysis / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000378:metabolism; D013457:Sulfur Compounds / Q000032:analysis / Q000378:metabolism
D006219:Halogens; D008670:Metals; D004364:Pharmaceutical Preparations; D013457:Sulfur Compounds