Localized therapeutic release via an amine-functionalized poly-p-xylene microfilm device.
Developing biocompatible polymeric platforms for drug delivery with enhanced localized activity represents a key facet of advanced interventional therapy. In this work, the drug-eluting potential of an amine-functionalized poly- p-xylene commonly known as Parylene A (4-amino(2,2)paracyclophane) was conducted with the microfilm device consisting of a primary base layer, drug film, and a secondary eluting layer presenting exposed amine groups which enhance the range of modifications that can be incorporated into the film. The murine macrophage cell line RAW 264.7 served as a cellular response to dexamethasone, a synthetic anti-inflammatory glucocorticoid and doxorubicin, an anticancer therapeutic. Decreased expression of NFkappa-B-mediated cytokines Interleukin-6 (IL-6) and Tumor Necrosis Factor-alpha (TNFalpha), resultant DNA fragmentation, and spectroscopic analysis revealed the efficient and localized drug-eluting properties of the Parylene A polymeric bilayer.
Robinson
Erik M
EM
Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, USA.
Lam
Robert
R
Pierstorff
Erik D
ED
Ho
Dean
D
D000588:Amines / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D053938:DNA Fragmentation; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology; D016503:Drug Delivery Systems; D005938:Glucocorticoids / Q000008:administration & dosage / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014992:Xylenes / Q000737:chemistry*
D000588:Amines; D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D016207:Cytokines; D005938:Glucocorticoids; D015850:Interleukin-6; D016328:NF-kappa B; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha; D014992:Xylenes; C031286:4-xylene; D003907:Dexamethasone; D004317:Doxorubicin