title: Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers.
abstract: An optimally effective AIDS vaccine would likely require the induction of both neutralizing antibody and cell-mediated immune responses, which has proven difficult to obtain in previous clinical trials. Here we report on the induction of human immunodeficiency virus type-1 (HIV-1)-specific immune responses in healthy adult volunteers that received the multi-gene, polyvalent, DNA prime-protein boost HIV-1 vaccine formulation, DP6-001, in a Phase I clinical trial. Robust cross-subtype HIV-1 specific T cell responses were detected in IFN-gamma ELISPOT assays. Furthermore, we detected high titer serum antibody responses that recognized a wide range of primary HIV-1 Env antigens and also neutralized pseudotyped viruses that express the primary Env antigens from multiple HIV-1 subtypes. These findings demonstrate that the DNA prime-protein boost approach is an effective immunization method to elicit both humoral and cell-mediated immune responses in humans, and that a polyvalent Env formulation could generate broad immune responses against HIV-1 viruses with diverse genetic backgrounds.
authors: Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA.
authors: Shixia
authors: S
authors: Wang
authors: Jeffrey S
authors: JS
authors: Kennedy
authors: Kim
authors: K
authors: West
authors: David C
authors: DC
authors: Montefiori
authors: Scott
authors: S
authors: Coley
authors: John
authors: J
authors: Lawrence
authors: Siyuan
authors: S
authors: Shen
authors: Sharone
authors: S
authors: Green
authors: Alan L
authors: AL
authors: Rothman
authors: Francis A
authors: FA
authors: Ennis
authors: James
authors: J
authors: Arthos
authors: Ranajit
authors: R
authors: Pal
authors: Phillip
authors: P
authors: Markham
authors: Shan
authors: S
authors: Lu
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D003429:Cross Reactions; D005260:Female; D015483:HIV Antibodies / Q000097:blood*; D015497:HIV-1 / Q000276:immunology*; D006805:Human Experimentation; D006801:Humans; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D013601:T-Lymphocytes / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D019444:Vaccines, DNA; D022223:Vaccines, Subunit; D007371:Interferon-gamma