title: Association between CAPN10 UCSNP-43 gene polymorphism and polycystic ovary syndrome in Chilean women.
abstract: BACKGROUND
  Studies have suggested that the Calpain-10 gene polymorphisms may play a role in polycystic ovary syndrome (PCOS) susceptibility. Thus, the aim of the present study was to evaluate the possible association between three single nucleotide polymorphisms (SNPs) in the calpain-10 gene (UCSNPs -43, -19, and -63) and polycystic ovary syndrome (PCOS) in Chilean women.
  
  
  METHODS
  Fifty women with PCOS (28.8+/-8.2 y) and 70 healthy women (28.6+/-8.6 y) were included in this study. Serum lipids, hormonal status, fasting glucose, oral glucose tolerance test (OGTT), insulin, HOMAIR, and uric acid levels were determined by conventional methods. The calpain-10 gene variants were detected by PCR and PCR-RFLP, respectively.
  
  
  RESULTS
  The presence of uncommon allele (A) for the UCSNP-43 was associated with increased risk of PCOS (odds ratio=1.93, 95% CI: 1.11-3.34). The UCSNP-63 (OR=1.11, 95% CI: 0.59-2.11) and UCSNP-19 (OR=0.93, 95% CI: 0.55-1.57) were not associated to PCOS. However, the PCOS women carrying the CC genotype for UCSNP-63 exhibited higher values of total cholesterol and LDL-C (P<0.05). Similarly, control women carrying the CC genotype showed higher serum levels of triglycerides, HDL-C and uric acid (P<0.05).
  
  
  CONCLUSIONS
  Our data suggest the contribution of CAPN10 UCSNP-43 gene polymorphism to PCOS in Chilean women. However, further studies with larger samples are necessary to confirm this observation.
authors: Laboratorio de Biología Molecular and Farmacogenética, Departamento de Ciencias Básicas, Facultad de Medicina, Universidad de La Frontera, Av. Francisco Salazar 01145, Casilla 54-D, Temuco, Chile.
authors: José Luis
authors: JL
authors: Márquez
authors: Alejandro
authors: A
authors: Pacheco
authors: Patricio
authors: P
authors: Valdés
authors: Luis A
authors: LA
authors: Salazar
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D001786:Blood Glucose / Q000378:metabolism; D002154:Calpain / Q000235:genetics*; D002677:Chile / Q000453:epidemiology; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005951:Glucose Tolerance Test; D006728:Hormones / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D011085:Polycystic Ovary Syndrome / Q000453:epidemiology* / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012306:Risk; D014527:Uric Acid / Q000097:blood; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D008078:Cholesterol, LDL; D006728:Hormones; D007328:Insulin; D008055:Lipids; D014527:Uric Acid; D004247:DNA; D002784:Cholesterol; D002154:Calpain; C415197:calpain 10