Reduced growth and integrin expression of prostate cells cultured with lycopene, vitamin E and fish oil in vitro.
Integrins are transmembrane proteins that facilitate the interaction of cells with the extracellular environment. They have also been implicated in cancer progression. The effects of nutrients thought to be involved in the prevention of prostate cancer on integrin expression have not been determined. Prostate cancer cell lines representing a range of malignancy from normal (RWPE-1) to highly invasive phenotypes (22Rv1 < LNCaP < PC-3) were cultured with or without lycopene (10 nM), vitamin E (5 microm) or fish oil (100 microm) for 48 h. Growth and integrin (alpha2beta1, alphavbeta3 and alphavbeta5) expression were assessed using Trypan Blue exclusion and monoclonal antibodies combined with flow cytometry. Vitamin E enhanced (P < 0.001) whereas fish oil reduced the growth of all the cell lines tested (P < 0.001). Lycopene had no effect on growth. All the malignant cell lines exhibited lower expression of alpha2beta1 with the addition of lycopene to culture media. Supplemental fish oil reduced alpha2beta1 in most invasive cell lines (LNCaP and PC-3). Each nutrient at physiological levels reduced integrins alphavbeta3 and alphavbeta5 in most invasive cell lines (PC-3). The results suggest that integrins may represent an additional target of bioactive nutrients and that the effects of nutrients may be dependent on the type of cell line used.
Bureyko
T
T
Alberta Institute for Human Nutrition, 4-10 Agriculture Forestry Center, University of Alberta, Edmonton, AB, Canada T6G 2E3.
Hurdle
H
H
Metcalfe
J B
JB
Clandinin
M T
MT
Mazurak
Vera C
VC
D000818:Animals; D002338:Carotenoids / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D003864:Depression, Chemical; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D005312:Fetal Blood / Q000378:metabolism; D005395:Fish Oils / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D038982:Integrin alpha2beta1 / Q000032:analysis / Q000378:metabolism; D039302:Integrin alphaVbeta3 / Q000032:analysis / Q000378:metabolism; D016023:Integrins / Q000032:analysis / Q000378:metabolism*; D000077276:Lycopene; D008297:Male; D011467:Prostate / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D019030:Receptors, Vitronectin / Q000032:analysis / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
D003470:Culture Media; D005395:Fish Oils; D038982:Integrin alpha2beta1; D039302:Integrin alphaVbeta3; D016023:Integrins; D019030:Receptors, Vitronectin; C064660:integrin alphaVbeta5; D014810:Vitamin E; D002338:Carotenoids; D000077276:Lycopene