Effects of haloperidol and risperidone on the expression of heat shock protein 70 in MK-801-treated rat C6 glioma cells.
Non-competitive N-methyl-D-aspartate (NMDA) receptor antagonists such as dizocilpine (MK-801) produce schizophrenia-like psychosis in humans and induce the expression of heat shock protein 70 (HSP70) in rats. The present study examines the effects of antipsychotic drugs, haloperidol and risperidone, on the expression of HSP70 produced by MK-801 in rat C6 glioma cells. After pretreating with haloperidol and risperidone for 1 h, 6 h, 24 h and 72 h, respectively, C6 glioma cells were cultivated again in MK-801 for 6 h, and then, the extent of HSP70 expression was measured by immunoblotting using anti-HSP70 monoclonal antibody. The expression of HSP70 induced by MK-801 significantly decreased as the duration of haloperidol pretreatment was extended (p=0.002). Risperidone also increasingly attenuated the expression of HSP70 produced by MK-801 as the duration of pretreatment grew longer (p=0.003). The present findings show that haloperidol and risperidone decrease the HSP70 expression in MK-801-treated rat C6 glioma cells. These results suggest that HSP70 and NMDA receptors may play a significant role in the pathophysiology of schizophrenia.
Roh
Kyungsoo
K
Department of Neuropsychiatry, Hanyang University College of Medicine, Seoul, Republic of Korea.
Roh
Sungwon
S
Yang
Byung-Hwan
BH
Lee
Jun-Seok
JS
Chai
Young Gyu
YG
Choi
Mi Ran
MR
Park
Yong Chon
YC
Kim
Dai-Jin
DJ
Kim
Daeho
D
Choi
Joonho
J
Kim
Seok Hyeon
SH
D000818:Animals; D045744:Cell Line, Tumor; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D005910:Glioma / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006220:Haloperidol / Q000494:pharmacology*; D016014:Linear Models; D051381:Rats; D018967:Risperidone / Q000494:pharmacology*; D013997:Time Factors
D018492:Dopamine Antagonists; D018691:Excitatory Amino Acid Antagonists; D018840:HSP70 Heat-Shock Proteins; D016291:Dizocilpine Maleate; D006220:Haloperidol; D018967:Risperidone