[Effects of FRZB on growth and metastasis of gastric cancer cell line SGC-7901].
OBJECTIVE
FRZB (frizzled motif associated with bone development), a member of the secreted frizzled related protein (sFRP) family, plays an important role in embryonic development. The expression of FRZB can suppress the invasion ability of prostate cancer by inhibiting the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. This study was to investigate the effects of FRZB on tumorigenicity and metastasis of gastric cancer cell line SGC-7901.


METHODS
FRZB expression vector and empty vector were constructed and transfected into SGC-7901 cells to obtain stable clones (SGC-7901/FRZB and SGC-7901/vector). Cell proliferation was detected by MTT assay. The tumorigenicity was investigated by soft agar colony formation test (in vitro) and xenograft mouse model (in vivo). The adhesive and invasive abilities of SGC-7901/FRZB cells were determined by Boyden chambers. The expression of MMPs in SGC-7901 cells was tested by immunocytochemistry.


RESULTS
The expression of FRZB was increased in SGC-7901/FRZB cells. Growth rate and colony formation rate of SGC-7901/FRZB cells were decreased to about 60% and 15% as compared with those of SGC-7901/vector cells, respectively. The rates of adhesion to collagenI, collagen IV, vitronectin, fibronectin and laminin were increased in SGC-7901/FRZB cells by 12.8%, 19.8%, 59.8%, 26.7% and 15.2% as compared with those in SGC-7901/vector cells, respectively. The positive dyeing of MMP-2, MMP-7 and MMP-9 in SGC-7901/FRZB cells was reduced. The numbers of invasive SGC-7901, SGC-7901/vector and SGC-7901/FRZB cells were 55.90+/-5.68, 54.80+/-6.97, 6.60+/-2.63, respectively.


CONCLUSIONS
FRZB exhibits antitumor ability in gastric cancer cell line SGC-7901 in vitro and in vivo, and decreases the expression of MMP-2, MMP-7 and MMP-9.
Qu
Ying
Y
Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, P. R. China.
Li
Jian-Fang
JF
Cai
Qu
Q
Liu
Bing-Ya
BY
Zhu
Zheng-Gang
ZG
D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D053505:Matrix Metalloproteinases, Secreted / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014162:Transfection; D047368:Tumor Burden; D000099274:Frizzled-Related Proteins
D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D020778:Matrix Metalloproteinase 2; D020783:Matrix Metalloproteinase 7; D020780:Matrix Metalloproteinase 9; D053505:Matrix Metalloproteinases, Secreted; D000099274:Frizzled-Related Proteins