Development of a biochip using antibody-coated gold nanoparticles to detect specific bioparticles.
This study developed a method of detecting bioparticles such as Salmonella that exist in the biological samples. The method employed a substrate with interlaced comb-like electrodes into which the mixtures of biological samples and antibody-coated gold nanoparticles were added. The alternative signals with appropriate frequency bands were then conducted into the comb-like electrodes to change the dielectrophoresis force. The gold-modified Salmonella can be adsorbed on the edges of the electrodes and isolated from various biological samples. The impedance of the adsorbed Salmonella on the edges of the electrodes was measured and comparison of the impedance between the electrodes with and without Salmonella can quantify the amount of the adsorbed Salmonella.
Huang
Jung-Tang
JT
Institute of Mechatronics Engineering, National Taipei University of Technology, and Taiwan Adventist Hospital, 1, Sec. 3, Zhong Xiao E. Rd., Taipei, 106, Taiwan. jthuang@ntut.edu.tw
Hou
Shao-Yi
SY
Fang
Shiuh-Bin
SB
Yu
Hua-Wei
HW
Lee
Hung-Chan
HC
Yang
Chu-Zen
CZ
D000906:Antibodies / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012475:Salmonella / Q000737:chemistry / Q000302:isolation & purification*
D000906:Antibodies; D006046:Gold