title: The radioprotective effects of the hexane and ethyl acetate extracts of Callophyllis japonica in mice that undergo whole body irradiation.
abstract: The radioprotective activity of extracts from the red seaweed Callophyllis (C.) japonica was investigated in mice that underwent whole-body exposure to gamma radiation. A methanol extract of C. japonica and its fractions [hexane, ethyl acetate (EtOAc), butanol and the remaining H2O] were used. Each fraction (100 mg/kg body weight) was administered intraperitoneally (i.p.) 2 times into the BALB/c mice, once at 1 and once at 24 h before exposure to 9 Gray (Gy) of gamma radiation. Pre-irradiation administration of the hexane and EtOAc fractions saved the mice, with their survival rates being greater than 80% at 30 days post-irradiation; the mice that were pretreated with the other fractions showed survival rates lower than 20% over the same time period. To examine the effect of each C. japonica fraction on the survival of intestinal and bone marrow stem cells, the number of intestinal crypts and bone marrow cells in the gamma-irradiated mice were examined. Pre-treatment of mice (i.p., 100 mg/kg body weight at 1 and 24 h before irradiation) with the hexane or EtOAc fraction prior to 6-Gy irradiation significantly protected the number of jejunal crypts and bone marrow cells at 9 days after irradiation. These findings suggest that certain extracts from C. japonica, when they are administered prior to irradiation, play an important role in the survival of irradiated mice, and this is possibly due to the extracts protecting the hematopoietic cells and intestinal stem cells against gamma irradiation.
authors: College of Veterinary Medicine, and the Research Institute for Subtropical Agriculture and Biotechnology, Cheju National University, Jeju 690-756, Korea.
authors: Jeongtae
authors: J
authors: Kim
authors: Changjong
authors: C
authors: Moon
authors: Heechul
authors: H
authors: Kim
authors: Jinwoo
authors: J
authors: Jeong
authors: Juyeon
authors: J
authors: Lee
authors: Jihoon
authors: J
authors: Kim
authors: Jin Won
authors: JW
authors: Hyun
authors: Jae Woo
authors: JW
authors: Park
authors: Mi Yeon
authors: MY
authors: Moon
authors: Nam Ho
authors: NH
authors: Lee
authors: Sung Ho
authors: SH
authors: Kim
authors: Youngheun
authors: Y
authors: Jee
authors: Taekyun
authors: T
authors: Shin
mesh_terms: D000085:Acetates; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects*; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005720:Gamma Rays; D006586:Hexanes; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D007583:Jejunum / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000517:prevention & control; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012624:Seaweed; D014916:Whole-Body Irradiation / Q000662:veterinary*
chemical_list: D000085:Acetates; D006586:Hexanes; D010936:Plant Extracts; D011837:Radiation-Protective Agents; C026385:n-hexane; C007650:ethyl acetate