title: Nucleophosmin mutations in Chinese adults with acute myelogenous leukemia.
abstract: Recently, mutations in the nucleophosmin (NPM1) gene were detected in 50-60% of adult acute myelogenous leukemia (AML) patients, mainly with a normal karyotype. In this study, we detected typical NPM1 mutations (types A, B, D) in untreated Chinese AML patients using real-time quantitative polymerase chain reaction (RQ-PCR) followed by sequence analysis. The detection rate of NPM1 mutations in 220 AML patients was 16.4%, including 107 (14.2%) with the French-American-British (FAB) subtype M2, 43 (2.3%) with M3, and 52 (30.8%) with M4/M5. Only one case each with an NPM1 mutation was detected in four M0, seven M1, five M6, and two M7 cases. Eight patients were followed up after treatment, and five patients in hematologic remission continued to test negative for NPM1 mutations within 2-14 months of follow-up. Sequence analysis revealed that all the 36 positive cases were heterozygous for the mutation with 4-bp insertions at nt 959; the 36 cases included 29 (80.6%) cases with type A, four (11.1%) cases with type B, and one rare DD-3 mutation. We also detected two new mutations, namely, CTCG and CAAG insertions, named BJ-01 and BJ-02, respectively. Further, 38.9% (14/36) patients with NPM1 mutations simultaneously exhibited internal tandem duplications in the FLT3 gene, and 66.7% (22/33) patients did not express CD34. The results demonstrated that RQ-PCR was a reliable and sensitive method for detecting NPM1 mutations, for screening AML, and for the quantitative analysis of minimal residual diseases.
authors: Peking University Institute of Hematology and People's Hospital, Beijing, China. celiar@public3.bta.net.cn
authors: Guo-Rui
authors: GR
authors: Ruan
authors: Jin-Lan
authors: JL
authors: Li
authors: Ya-Zhen
authors: YZ
authors: Qin
authors: Ling-Di
authors: LD
authors: Li
authors: Min
authors: M
authors: Xie
authors: Yan
authors: Y
authors: Chang
authors: Yan
authors: Y
authors: Zhang
authors: Yan-Rong
authors: YR
authors: Liu
authors: Bin
authors: B
authors: Jiang
authors: Shan-Shan
authors: SS
authors: Chen
authors: Xiao-Jun
authors: XJ
authors: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D005260:Female; D005500:Follow-Up Studies; D018628:Gene Dosage / Q000235:genetics; D006801:Humans; D007201:Indicator Dilution Techniques; D015470:Leukemia, Myeloid, Acute / Q000453:epidemiology* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D000090243:Nucleophosmin; D010957:Plasmids / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D000090243:Nucleophosmin