High-resolution 14N-edited 1H-13C correlation NMR experiment to study biological solids.
It was recently shown that nuclear magnetic resonance (NMR) spectra of nitrogen-14 (spin I=1) can be obtained by indirect detection via spin S=1/2 nuclei in powders spinning at the magic angle. An increased number of solid-state NMR methods are now available to tailor sequences for specific purposes, e.g., hetero-nuclear dipolar recoupling or homo-nuclear dipolar decoupling schemes. Here, we combine the latest recoupling and decoupling techniques to obtain high-resolution (1)H-(13)C through-space correlation spectra, where only the correlation peaks of those carbons close to nitrogen nuclei are observed. The experiment is demonstrated on a (13)C enriched l-histidine. HCl x H(2)O powder sample.
Amoureux
Jean-Paul
JP
Unité de Catalyse et Chimie du Solide, UMR-CNRS 8181, Université de Lille 1, 59652 Villeneuve d'Ascq cedex, France. jean-paul.amoureux@univ-lille1.fr
Trébosc
Julien
J
Hu
Bingwen
B
Halpern-Manners
Nicholas
N
Antonijevic
Sasa
S
D001704:Biopolymers / Q000032:analysis* / Q000737:chemistry*; D002247:Carbon Isotopes / Q000737:chemistry*; D006639:Histidine / Q000032:analysis* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009584:Nitrogen / Q000737:chemistry*; D011522:Protons; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001704:Biopolymers; D002247:Carbon Isotopes; D011522:Protons; D006639:Histidine; D009584:Nitrogen