title: In vitro differentiation of human adipose tissue-derived stem cells into cells with pancreatic phenotype by regenerating pancreas extract.
abstract: Pancreas extract from regenerating pancreas after partial pancreatectomy is known to contain factors that induce islet neogenesis in animals with streptozotocin (STZ)-induced diabetes [A.A. Hardikar, R.R. Bhonde, Modulating experimental diabetes by treatment with cytosolic extract from the regenerating pancreas, Diabetes Res. Clin. Pract. 46 (1999) 203-211]. In this study, we evaluate the effects of regenerating pancreas extract (RPE) from 90% partially pancreatectomized rats on induction of pancreatic differentiation of human adipose tissue-derived stem cells (hASCs). We found that undifferentiated hASCs expressed OCT-3/4, Nanog, and REX-1, markers of embryonic stem cells (ESCs). Genes involved in early pancreas development showed increased expression in RPE-treated culture. Sox17 and IPF-1 were expressed only in RPE-treated culture. Immunocytochemical analysis showed C-peptide-positive cells in RPE-treated culture but not in undifferentiated hASCs. In conclusion, hASCs have the characteristics of ESCs and the potential to differentiate into pancreas cell lineages phenotypically in response to RPE.
authors: Laboratory of Cell Therapy, Asan Institute for Life Sciences, Asan Medical Center, Seoul 138-736, Republic of Korea.
authors: Jiyeon
authors: J
authors: Lee
authors: Duck-Jong
authors: DJ
authors: Han
authors: Song-Cheol
authors: SC
authors: Kim
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D002457:Cell Extracts; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006609:High Mobility Group Proteins / Q000096:biosynthesis; D018398:Homeodomain Proteins / Q000096:biosynthesis; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000502:physiology*; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D010641:Phenotype; D051381:Rats; D012038:Regeneration; D055760:SOXF Transcription Factors; D015534:Trans-Activators / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis
chemical_list: D002457:Cell Extracts; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D018398:Homeodomain Proteins; D051741:Kruppel-Like Transcription Factors; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C109188:SOX17 protein, human; D055760:SOXF Transcription Factors; D015534:Trans-Activators; D014157:Transcription Factors; C507976:ZFP42 protein, human; C084010:pancreatic and duodenal homeobox 1 protein