Long-term durability, tissue regeneration and neo-organ growth during skeletal maturation with a neo-bladder augmentation construct.
OBJECTIVE
To comparatively evaluate bladder regeneration following 80% cystectomy and augmentation using a synthetic biopolymer with autologous urothelial and smooth muscle cells (autologous neo-bladder augmentation construct [construct]) or autotransplantation of native bladder (reimplanted native urinary bladder [reimplant]) in canines.


METHODS
Voiding function, urodynamic assessment and neo-organ capacity-to-body-weight ratio (C:BW) were assessed longitudinally for a total of 24 months following trigone-sparing augmentation cystoplasty in juvenile canines.


RESULTS
Within 30 days postimplantation, hematology and urinalysis returned to baseline. Constructs and reimplants yielded neo-organs with statistically equivalent urodynamics and histology. Linear regression analysis of C:BW showed that constructs regained baseline slope and continued to adapt with animal growth.


CONCLUSIONS
Constructs and reimplants regained and maintained native bladder histology by 3 months, capacity at 3-6 months and compliance by 12-24 months. Furthermore, construct C:BW demonstrated the ability of regenerated bladder to respond to growth regulation.
Jayo
Manuel J
MJ
Tengion, Inc., 3929 Westpoint Blvd., Suite G, Winston-Salem, NC 27103, USA.
Jain
Deepak
D
Ludlow
John W
JW
Payne
Richard
R
Wagner
Belinda J
BJ
McLorie
Gordon
G
Bertram
Timothy A
TA
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001704:Biopolymers / Q000737:chemistry; D017690:Cell Transplantation; D004285:Dogs; D005260:Female; D008297:Male; D008954:Models, Biological; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D011108:Polymers / Q000737:chemistry; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods; D001743:Urinary Bladder / Q000473:pathology* / Q000502:physiology; D014563:Urodynamics
D001672:Biocompatible Materials; D001704:Biopolymers; D011108:Polymers