Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis.
Ankylosing spondylitis (AS) is a chronic inflammatory arthritis that predominantly affects the axial skeleton in adolescent patients. The natural history of the disease remains poorly characterized. In this study, we combined GC-MS and LC-MS techniques to evaluate the major metabolic changes in the plasma of AS patients in view of metabonomics. Univariate and multivariate analysis were employed for altered metabolite comparison and pattern recognition. Application of supervised partial least-squares discrminant analysis to either GC-MS or LC-MS data allowed accurate discrimination of AS patients from normal controls, demonstrating its potential diagnostic utilization. In addition, AS patients presented elevated plasma concentrations of proline, glucose, phosphate, urea, glycerol, phenylalanine and homocysteine but reduced levels of phosphocholines, tryptophan and a bipeptide - phenylalanyl-phenylalanine. In the context of their involved metabolic pathways, the identified metabolites were discussed accordingly. This investigation primarily proved that integrated chromatography-mass spectrometry and integrated uni- and multi-variate statistical analysis facilitated metabonomics to be a more promising tool in disease research.
Gao
Peng
P
National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Lu
Chen
C
Zhang
Fengxia
F
Sang
Ping
P
Yang
Dawei
D
Li
Xiang
X
Kong
Hongwei
H
Yin
Peiyuan
P
Tian
Jing
J
Lu
Xin
X
Lu
Aiping
A
Xu
Guowang
G
D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002853:Chromatography, Liquid / Q000379:methods; D016002:Discriminant Analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D008970:Molecular Weight; D010363:Pattern Recognition, Automated; D012680:Sensitivity and Specificity; D013167:Spondylitis, Ankylosing / Q000097:blood*
D001798:Blood Proteins