[Preparation of three capsaicinoid components using preparative high performance liquid chromatography].
A method was established for the isolation of three capsaicinoid components from capsaicinoids using preparative high performance liquid chromatography (Pre-HPLC). A preparative PRC-ODS column (250 mm x 21.5 mm, 13 microm) was used in the preparation process. By isocratic elution with methanol-water (70 : 30, v/v) as the mobile phase at a flow rate of 15 mL/min followed by concentration, three capsaicinoid components were obtained from 80% capsaicinoids, and subsequently identified by 1H NMR (nuclear magnetic resonance) and EI-MS (electron impact-mass spectrometry) as nordihydrocapsaicin, capsaicin and dihydrocapsaicin, and the purities of the three components analyzed by HPLC were 98.12%, 99.93% and 100.7% with yields of 60.1%, 58.9% and 72.3%, respectively.
Dong
Xinrong
X
College of Science, Hunan Agricultural University, Changsha 410128, China. xinrong108@yahoo.com.cn
Liu
Zhonghua
Z
Li
Benxiang
B
Wang
Kunbo
K
D053000:Analytic Sample Preparation Methods / Q000379:methods*; D002211:Capsaicin / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
D012997:Solvents; D002211:Capsaicin