title: Nebrodeolysin, a novel hemolytic protein from mushroom Pleurotus nebrodensis with apoptosis-inducing and anti-HIV-1 effects.
abstract: A novel hemolysin was isolated from the edible mushroom Pleurotus nebrodensis by ion exchange and gel filtration chromatography on DEAE-Sepharose and Sephacryl S-100. The hemolysin from Pleurotus nebrodensis hemolysin (nebrodeolysin) is a monomeric protein with a molecular weight of approximately 27 kDa as determined by gel filtration and SDS-PAGE. Nebrodeolysin exhibited remarkable hemolytic activity towards rabbit erythrocytes and caused efflux of potassium ions from erythrocytes. Subsequently, this hemolysin showed strong cytotoxicity against Lu-04, Bre-04, HepG2, L929, and HeLa cells. It was also found that this hemolysin induced apoptosis in L929 and HeLa cells as evidenced by microscopic observations and DNA ladder, respectively. Moreover, this hemolysin was shown to possess anti-HIV-1 activity in CEM cell culture.
authors: College of Life Sciences, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064, China.
authors: Hui
authors: H
authors: Lv
authors: Yang
authors: Y
authors: Kong
authors: Qing
authors: Q
authors: Yao
authors: Bo
authors: B
authors: Zhang
authors: Fang-Wei
authors: FW
authors: Leng
authors: He-Jiao
authors: HJ
authors: Bian
authors: Jan
authors: J
authors: Balzarini
authors: Els
authors: E
authors: Van Damme
authors: Jin-Ku
authors: JK
authors: Bao
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D048690:Fruiting Bodies, Fungal; D005656:Fungal Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D015658:HIV Infections / Q000188:drug therapy*; D015497:HIV-1 / Q000187:drug effects; D006460:Hemolysin Proteins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D053878:Hemolytic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy*; D008517:Phytotherapy; D020076:Pleurotus / Q000737:chemistry*; D011817:Rabbits
chemical_list: D019380:Anti-HIV Agents; D000970:Antineoplastic Agents; D005656:Fungal Proteins; D006460:Hemolysin Proteins; D053878:Hemolytic Agents