Isolation and characterization of a human apoptosis-inducing gene with yeast two-hybrid system.
asy gene is a novel apoptosis-inducing gene, but its mechanism is unclear. To investigate the mechanism ofasy inducing apoptosis, a novel gene encoding ASY interacting protein (asyip) is isolated from human lung cell line (WI-38) cDNA library with yeast two-hybrid system. Theasyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 and 2.7 kb in various human tissues at different levels. Sequence analysis of full-length cDNA reveals that the two alternative transcripts ofasyip gene contain common 5' end and different 3' end, and share a common open reading frame encoding a polypeptide of 236 amino acids. Two protein kinase C phosphorylation sites and two casein kinase II phosphorylation sites are found in ASYIP amino acid sequence. Two highly hydrophobic regions encoding potentially two transmembrane domains are present. The ASYIP protein contains a C-terminal endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu). Immunoprecipitation assay confirmed the interaction of ASY and ASYIP in mammalian cells. Compared withasy gene, overexpression ofasyip gene can inhibit growth of tumor cell Saos2 and induce cell apoptosis with a low efficiency.
Qi
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Institute of Virology, Wuhan University, 430072, Wuhan, China.
Qi
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Y
Masuo
Y
Y
Liu
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Q