Expression profile of metastasis-related genes in invasive oral cancers.
We used suppression subtractive hybridization (SSH) and oligonucleotide microarray to differentiate expression profiles of metastasis-related genes and to evaluate their clinical significance in patients with invasive oral cancer (OCa). Overexpression of the specific genes was confirmed by reverse transcription-PCR (RT-PCR). Cells expressing the gene were identified by immunohistochemistry in pathology specimens. Clinical correlation and significance were analyzed statistically. Using these methods, we detected increased expressions of MMP-1, -3, -7, -9, -10 and interleukin (IL)-8 in invasive OCa. Moreover, our data showed that overexpressions of MMP-1, -3, -7, -10 and IL-8 were associated with reduced survival.
Chiang
Y Y
YY
Department of Dental Laboratory Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan. YYChiang668@gmail.com
Tsai
M H
MH
Lin
T Y
TY
Chiang
I P
IP
D000328:Adult; D000368:Aged; D002470:Cell Survival; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000235:genetics*; D053208:Kaplan-Meier Estimate; D008297:Male; D053505:Matrix Metalloproteinases, Secreted / Q000235:genetics*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation
C508606:CXCL8 protein, human; D016209:Interleukin-8; D053505:Matrix Metalloproteinases, Secreted