Cardiolipin increases in chromatophores isolated from Rhodobacter sphaeroides after osmotic stress: structural and functional roles.
Chromatophores isolated from cells of Rhodobacter sphaeroides exposed to hypertonic solutions were enriched in cardiolipin (CL). Because CL levels are raised by increasing the incubation time of R. sphaeroides in hypertonic solutions, it was possible to isolate chromatophores containing different CL amounts by starting from cells incubated in hypertonic solutions for different times. The functionality and stability of the photosynthetic proteins in chromatophore membranes having different CL levels were investigated. Reaction center (RC) stabilization with respect to thermal denaturation and photoxidative damage was observed by flash photolysis and fluorescence emission experiments in CL-enriched chromatophores. To gain detailed information about the structures of endogenous CLs, this lipid family was isolated and purified by preparative TLC, and characterized by high-resolution mass spectrometry. We conclude that osmotic shock can be used as a tool to modulate CL levels in isolated chromatophores and to change the composition of the RC lipid annulus, avoiding membrane artifacts introduced by the use of detergents.
De Leo
Vincenzo
V
Department of Chemistry, Università di Bari, Bari, Italy.
Catucci
Lucia
L
Ventrella
Andrea
A
Milano
Francesco
F
Agostiano
Angela
A
Corcelli
Angela
A
D020130:Bacterial Chromatophores / Q000378:metabolism*; D002308:Cardiolipins / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D009995:Osmosis; D009997:Osmotic Pressure; D010782:Photolysis; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013997:Time Factors
D002308:Cardiolipins