title: Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging.
abstract: Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3+/-0.4 ms were achieved with the single-layer microfluidic device.
authors: Chemical Biology Centre, Department of Chemical Engineering, Imperial College London, London, UK.
authors: Tom
authors: T
authors: Robinson
authors: Prashant
authors: P
authors: Valluri
authors: Hugh B
authors: HB
authors: Manning
authors: Dylan M
authors: DM
authors: Owen
authors: Ian
authors: I
authors: Munro
authors: Clifford B
authors: CB
authors: Talbot
authors: Christopher
authors: C
authors: Dunsby
authors: John F
authors: JF
authors: Eccleston
authors: Geoff S
authors: GS
authors: Baldwin
authors: Mark A A
authors: MA
authors: Neil
authors: Andrew J
authors: AJ
authors: de Mello
authors: Paul M W
authors: PM
authors: French
mesh_terms: D002138:Calibration; D004867:Equipment Design; D005453:Fluorescence; D021621:Imaging, Three-Dimensional; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D044085:Microfluidics; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors