D-fructose detection based on the direct heterogeneous electron transfer reaction of fructose dehydrogenase adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode.
Multi-walled carbon nanotubes (MWCNTs) were synthesized on platinum plate electrodes by the chemical vapor deposition (CVD) method. From the results of X-ray photoelectron spectroscopy and voltammetric investigation, the iron nanoparticles used as a catalyst for the MWCNT synthesis were enclosed with MWCNTs. The MWCNTs synthesized on the Pt plate (MWCNTs/Pt) electrode were immediately immersed into solutions of d-fructose dehydrogenase (FDH) to immobilize the enzyme onto the MWCNTs/Pt electrode surfaces. After the FDH was immobilized onto the MWCNTs/Pt electrode, a well-defined catalytic oxidation current based on FDH was observed from ca. -0.15V (versus Ag/AgCl/sat'd KCl), which was close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current versus substrate, the calibration range for the fructose concentration was up to ca. 40mmoldm(-3), and the apparent Michaelis-Menten constant was evaluated to be 11+/-1mmoldm(-3).
Tominaga
Masato
M
Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.
Nomura
Shinya
S
Taniguchi
Isao
I
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005632:Fructose / Q000032:analysis* / Q000737:chemistry; D008839:Microelectrodes; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010088:Oxidoreductases / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D005632:Fructose; D010984:Platinum; D010088:Oxidoreductases