Two different clades of copia-like retrotransposons in the red alga, Porphyra yezoensis.
A copia-like retrotransposon referred to as PyRE1G1 was isolated from the genome of the red alga Porphyra yezoensis. PyRE1G1 is 4807 bp in length, with 204 bp long terminal repeats (LTRs) at both ends. PyRE1G1 has an open reading frame of 1401 residues encoding gag, protease, integrase, reverse transcriptase (RT), and RNase H. From the order of gene arrangement of proteins, PyRE1G1 appears to be a copia-like retrotransposon. Genomic Southern blot analysis suggests that PyRE1G1 consists of a small gene family. From the phylogenetic tree of RT sequences, PyRE1G1 is grouped in the clade of usual copia elements and distinct from the previously isolated red algal copia-like gene PyRE10G in that the latter is closely related to a new clade of aquatic animal-specific copia-like retrotransposons.
Peddigari
Suresh
S
Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto 860-8555, Japan.
Zhang
Wenbo
W
Takechi
Katsuaki
K
Takano
Hiroyoshi
H
Takio
Susumu
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D044664:Porphyra / Q000145:classification / Q000235:genetics*; D018626:Retroelements / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020079:Terminal Repeat Sequences / Q000235:genetics
D018626:Retroelements