MOZ/TIF2-induced acute myeloid leukaemia in transgenic fish.
The inv(8)(p11q13) chromosomal abnormality, described in acute myeloid leukaemias (AML), fuses the histone acetyl-transferase (HAT) MYST3 (MOZ) gene with another HAT gene, NCOA2 (TIF2). We generated a transgenic zebrafish in which the MYST3/NCOA2 fusion gene was expressed under control of the spi1 promoter. An AML developed in 2 of 180 MYST3/NCOA2-EGFP-expressing embryos, 14 and 26 months after injection of the fusion gene in a one-cell embryo, respectively. This leukaemia was characterised by an extensive invasion of kidneys by myeloid blast cells. This model, which is the first zebrafish model of AML, demonstrates the oncogenic potency of MYST3/NCOA2 fusion gene.
Zhuravleva
Julia
J
Inserm UMR 866, Dijon, France.
Paggetti
Jérôme
J
Martin
Laurent
L
Hammann
Arlette
A
Solary
Eric
E
Bastie
Jean-Noël
JN
Delva
Laurent
L
D000818:Animals; D030801:Animals, Genetically Modified; D004195:Disease Models, Animal; D050939:Gene Fusion; D051548:Histone Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008845:Microinjections / Q000379:methods; D051958:Nuclear Receptor Coactivator 2 / Q000235:genetics* / Q000378:metabolism; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D015027:Zebrafish / Q000235:genetics
C498001:NCOA2 protein, human; D051958:Nuclear Receptor Coactivator 2; D015514:Oncogene Proteins, Fusion; D051548:Histone Acetyltransferases; C108712:KAT6A protein, human