[Regulatory genes of geldanamycin biosynthesis].
Two LAL family regulatory genes, gdmRI and gdmRII, were identified in the geldanamycin biosynthetic gene cluster of Streptomyces hygroscopicus 17997. Disruption of the two regulatory genes resulted in absolute elimination of geldanamycin biosynthesis. The complementation experiments using a single wild-type gene could restore geldanamycin production. These results indicated that both gdmRI and gdmRII were positive regulatory genes of the geldanamycin biosynthesis.
He
Weiqing
W
Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050, China.
Lei
Jian
J
Liu
Yuying
Y
Wang
Yiguang
Y
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D016227:Benzoquinones / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors; D047029:Lactams, Macrocyclic / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D012097:Repressor Proteins / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*
D000900:Anti-Bacterial Agents; D016227:Benzoquinones; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D012097:Repressor Proteins; D015534:Trans-Activators; C064778:LuxR autoinducer binding proteins; D011505:Protein-Tyrosine Kinases; C001277:geldanamycin