Ultrastructural analysis of the cristae ampullares in the squirrel monkey (Saimiri sciureus).
Type I hair cells outnumber type II hair cells (HCs) in squirrel monkey (Saimiri sciureus) cristae by a nearly 3:1 ratio. Associated with this type I HC preponderance, calyx fibers make up a much larger fraction of the afferent innervation than in rodents (Fernández et al. [1995] J. Neurophysiol. 73:1253-1269). To study how this affects synaptic architecture, we used disector methods to estimate various features associated with type I and type II HCs in central (CZ) and peripheral (PZ) zones of monkey cristae. Each type I HC makes, on average, 5-10 ribbon synapses with the inner face of a calyx ending. Inner-face synapses outnumber those on calyx outer faces by a 40:1 ratio. Expressed per afferent, there are, on average, 15 inner-face ribbon synapses, 0.38 outer-face ribbons, and 2.6 efferent boutons on calyx-bearing endings. Calyceal invaginations per type I HC range from 19 in CZ to 3 in PZ. For type II HCs, there are many more ribbons and afferent boutons in PZ than in CZ, whereas efferent innervation is relatively uniform throughout the neuroepithelium. Despite outer-face ribbons being more numerous in chinchilla than in squirrel monkey, afferent discharge properties are similar (Lysakowski et al. [1995] J. Neurophysiol. 73:1270-1281), reinforcing the importance of inner-face ribbons in synaptic transmission. Comparisons across mammalian species suggest that the prevalence of type I HCs is a primate characteristic, rather than an arboreal life-style adaptation. Unlike cristae, type II HCs predominate in monkey maculae. Differences in hair-cell counts may reflect the stimulus magnitudes handled by semicircular canals and otolith organs.
Lysakowski
Anna
A
Department of Anatomy and Cell Biology, University of Illinois at Chicago, Illinois 60612, USA. alysakow@uic.edu
Goldberg
Jay M
JM
D000818:Animals; D002682:Chinchilla / Q000033:anatomy & histology; D018069:Hair Cells, Vestibular / Q000648:ultrastructure*; D008297:Male; D010037:Otolithic Membrane / Q000648:ultrastructure; D012453:Saimiri / Q000033:anatomy & histology*; D054776:Semicircular Ducts / Q000648:ultrastructure*; D013569:Synapses / Q000648:ultrastructure; D009435:Synaptic Transmission