Characters of DNA constitution in the rye B chromosome.
We have used chromosome microdissection and microcloning to construct a DNA library of the entire B chromosome (B) of rye. New rye B-specific sequences have been screened from this pool, blasted with other sequences and analyzed to elucidate the characters of DNA constitution and the possible pathway of the origin of the rye B chromosome. We report the discovery of a new sequence that is specific to the rye B centromere.
Long
Hong
H
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Qi
Zhong-Xia
ZX
Sun
Xiao-Ming
XM
Chen
Cheng-Bin
CB
Li
Xiu-Lan
XL
Song
Wen-Qin
WQ
Chen
Rui-Yang
RY
D001483:Base Sequence; D015139:Blotting, Southern; D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D018628:Gene Dosage; D042282:Microdissection; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012434:Secale / Q000235:genetics*
D018744:DNA, Plant