Molecular characterization, expression and association analysis of the porcine CMYA4 gene with carcass traits.
CMYA4 (cardiomyopathy-associated 4) gene plays an important role in thick filament assembly. In this study, we obtained the mRNA sequence including the full coding sequence and the partial 5' untranslated region of the porcine CMYA4 gene by using the rapid amplification of cDNA ends and reverse transcriptase polymerase chain reaction (RT-PCR) and the sequence was deposited in the GenBank nucleotide database (DQ_286571). The human (NM_173167) and mouse (NM_178680) homologues have a 91% and 87% identity with the porcine CMYA4 gene, respectively. The sequence contains an open reading frame encoding 930 amino acid residues, and the amino terminus of the predicted CMYA4 protein contains three tandem repeats belonging to the tetratricopeptide repeat family. Semi-quantitative RT-PCR results showed that the porcine CMYA4 gene is expressed exclusively in striated muscle tissue. An A558G single nucleotide polymorphism in the CMYA4 intron 15 detected as an MspI PCR-restriction fragment length polymorphism showed allele frequency differences among 225 unrelated pigs from six breeds. Association of the genotypes with growth and carcass traits showed that different genotypes of the CMYA4 gene were significantly associated with the backfat thickness of the area between sixth and seventh ribs (p < 0.05) and backfat thickness at the shoulder (p < 0.05).
Xu
X L
XL
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, PR China.
Li
K
K
Peng
Z Z
ZZ
Zhao
S H
SH
Yu
M
M
Fan
B
B
Zhu
M J
MJ
Xu
S P
SP
Du
Y Q
YQ
Liu
B
B
D000818:Animals; D001823:Body Composition / Q000235:genetics; D050218:Body Fat Distribution; D005787:Gene Frequency; D005838:Genotype; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D013552:Swine / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism
D009124:Muscle Proteins