Regulation of matrix metalloproteinases (MMP)-2/-9 expression in eosinophilic chronic rhinosinusitis--cell culture by interleukin-5 and -13?
BACKGROUND
Eosinophils are a prominent immunological feature of chronic rhinosinusitis (CRS). Cytokines in the respiratory mucosa may be the key to upper airway pathophysiology. Matrix metalloproteinases (MMP) represent an entire group of Zn2+ dependent endopeptidases with the potential to alter the extracellular matrix (ECM). In this study epithelial cultures of CRS were treated with interleukin (IL)-5 or IL-13 and subsequent levels of metalloproteinases were determined.


METHODS
The cells for CRS culture were obtained from patients undergoing functional endoscopic sinus surgery. After 8-72 hours incubation with 0.2-0.4 ng/ml IL-5 or 3-6 ng/ml IL-13, the expression of the MMP-2 and -9 in the CRS cultures was analysed.


RESULTS
After 72 hours incubation with IL-5, the relative levels of MMP-2 showed no significant alteration in protein expression in comparison with the control groups. Incubation with IL-13 revealed a statistically insignificant decrease of the relative MMP-9 expression in ECRS compared to the control group (p>0.1).


CONCLUSIONS
Alterations of MMP-2 and -9 expression may play a role in ECRS, but the association with IL-5 and IL-13 remains unclear.
Sauter
Alexander
A
Department of Otolaryngology, Head and Neck Surgery, University Hospital Mannheim, Mannheim, Germany. alexander.sauter@googlemail.com
Stern-Straeter
Jens
J
Sodha
Selina
S
Hörmann
Karl
K
Naim
Ramin
R
D016207:Cytokines / Q000378:metabolism; D004724:Endoscopy; D004797:Enzyme-Linked Immunosorbent Assay; D004802:Eosinophilia / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D018793:Interleukin-13 / Q000378:metabolism*; D015848:Interleukin-5 / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D008954:Models, Biological; D010539:Permeability; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
D016207:Cytokines; D018793:Interleukin-13; D015848:Interleukin-5; D019715:Tissue Inhibitor of Metalloproteinase-1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9