[Cryopreservation strengthens procoagulative activities of platelets].
The aim of this study was to explore the potential relationship between the enhancement of instant hemostatic function in vivo of cryopreserved platelets and its procoagulative related molecule activities. The ability of platelet binding factor V density of GPIb-IX-V (CD42a) at platelet member surface were detected by flow cytometry, the clotting time induced by activated platelets were evaluated by coagulometer and platelet count, MPV and PDW were measured by hemocytometer before and after fresh platelets were cryopreserved. The results showed that the clotting time induced by activated cryopreserved platelets decreased by 43.9%, even quicker than that induced by fresh platelets; the fluorescence intensity of cryopreserved platelet binding factor V increased by 117%, more than that of fresh platelets binding factor V; the GPIb-IX-V (CD42a) density at cryopreserved platelet membrane surface increased by 32%, higher than that at fresh platelet surface. It is concluded that the enhancement of instant hemostatic function in vivo of cryopreserved platelet may be related to higher expression of procoagulative molecules or to their enhanced activity and rapid hemostatic effect.
Ouyang
Xi-Lin
XL
Department of Blood Transfusion, The First Affiliated Hospital of PLA General Hospital, Beijing 100037, China.
Zhou
Dan
D
Wu
Jing-Hui
JH
Wang
Li-Hua
LH
Hao
Jun
J
Liu
Jing-Han
JH
D001777:Blood Coagulation / Q000502:physiology; D001779:Blood Coagulation Factors / Q000378:metabolism; D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000032:analysis
D001779:Blood Coagulation Factors; D019038:Platelet Glycoprotein GPIb-IX Complex; C037685:platelet factor V