[Altered expression of survival (CD127) and death (Fas) receptors in total, naïve and memory CD8 T lymphocytes from human immunodeficiency virus infected patients: possible implications for progression of infection].
We studied the ex vivo and in vitro expression of CD95/Fas and CD127 receptors in total, naive and memory CD8+ T cells from HIV infected patients with different blood counts of CD4+ T cells. In addition, spontaneous and induced apoptosis were determined in vitro using a viral antigen (Env), along with an evaluation of their specific proliferative capacity. The obtained results demonstrated that patients with low counts of CD4+ T cells (CD4 < 250/microL), showed ex vivo, a high expression of CD95/Fas and a low expression of CD127 in all CD8+ T cell subgroups, as compared with patients with bigger counts of CD4+ T cells in blood (CD4> 250/microL). In vitro analyses using Env antigen showed that CD8+ T cells displayed a similar expression of both receptors, with a higher incidence of spontaneous and induced apoptosis and a diminished proliferative capacity as compared with controls. Results indicate how the progression of VIH infection in non-treated patients is related to a decrease of CD8+ T cells in blood, characterized by failures in their proliferative capacity and apoptosis frequency, which is demonstrated by the altered expression of CD127 and CD95/Fas expression.
Alfonzo
Miguel
M
Departamento de Ciencias Fisiológicas, Facultad de Medicina, Escuela de Medicina José María Vargas, Universidad Central de Venezuela, Caracas, Venezuela. alfonzmi@ucv.ve
Suárez
José
J
Díaz
Alexandra
A
Comegna
Mario
M
Valenzuela
Patricia
P
Sánchez
María
M
Hernández
Dimas
D
Hung
Alida
A
D000328:Adult; D017209:Apoptosis; D018791:CD4 Lymphocyte Count; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D005786:Gene Expression Regulation; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology; D006801:Humans; D053699:Interleukin-7 Receptor alpha Subunit / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
D053699:Interleukin-7 Receptor alpha Subunit; D019014:fas Receptor