title: Normal ovarian surface epithelial label-retaining cells exhibit stem/progenitor cell characteristics.
abstract: Ovulation induces cyclic rupture and regenerative repair of the ovarian coelomic epithelium. This process of repeated disruption and repair accompanied by complex remodeling typifies a somatic stem/progenitor cell-mediated process. Using BrdU incorporation and doxycycline inducible histone2B-green fluorescent protein pulse-chase techniques, we identify a label-retaining cell population in the coelomic epithelium of the adult mouse ovary as candidate somatic stem/progenitor cells. The identified population exhibits quiescence with asymmetric label retention, functional response to estrous cycling in vivo by proliferation, enhanced growth characteristics by in vitro colony formation, and cytoprotective mechanisms by enrichment for the side population. Together, these characteristics identify the label-retaining cell population as a candidate for the putative somatic stem/progenitor cells of the coelomic epithelium of the mouse ovary.
authors: Pediatric Surgical Research Laboratories, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
authors: Paul P
authors: PP
authors: Szotek
authors: Henry L
authors: HL
authors: Chang
authors: Kristen
authors: K
authors: Brennand
authors: Akihiro
authors: A
authors: Fujino
authors: Rafael
authors: R
authors: Pieretti-Vanmarcke
authors: Cristina
authors: C
authors: Lo Celso
authors: David
authors: D
authors: Dombkowski
authors: Frederic
authors: F
authors: Preffer
authors: Kenneth S
authors: KS
authors: Cohen
authors: Jose
authors: J
authors: Teixeira
authors: Patricia K
authors: PK
authors: Donahoe
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000166:cytology*; D030762:Estrous Cycle; D005260:Female; D049452:Green Fluorescent Proteins; D051379:Mice; D010053:Ovary / Q000166:cytology*; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology*
chemical_list: D049452:Green Fluorescent Proteins; D001973:Bromodeoxyuridine