[A confirmatory diagnosis of antibodies anti-Trypanosoma cruzi in donors referred by blood banks in Venezuela].
To establish the confirmatory diagnosis of Trypanosoma cruzi infection, at least two immunoserological tests (ELISA, Indirect hamaglutination, IH, Complement Fixation Test, CFT) were carried out in 254 donors, from public and private blood banks of Venezuela, during 48 months between 1997-1998 and 2003-2004, referred to the Immunology Section of the Tropical Medicine Institute in Caracas. Antibodies anti-T. cruzi were detected in 129/254 (50,79%) by ELISA-IgG or IH and CFT. The "artificial xenodiagnosis" was positive in 10/118 persons with positive confirmed serology. Of 129 donors found positive by the serological tests, 68 were living in the capital region and 61 in the interior of the country. Likewise 113 were born in the interior of the country, 8 in Caracas and 8 in Colombia. Of them, 12 individuals serologically confirmed declared to have donated blood in a minimum of 4 occasions before diagnosis. The present study emphasizes the importance of detection of antibodies against T. cruzi in the integral evaluation of blood donors, since many of them with antibodies anti-T. cruzi, have donated blood several times previous to diagnosis.
Díaz-Bello
Zoraida
Z
Sección de Inmunología, Instituto de Medicina Tropical, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela. diazzo@ucv.ve
Zavala-Jaspe
Reinaldo
R
Díaz-Villalobos
María
M
Mauriello
Luciano
L
Maekelt
Alberto
A
de Noya
Belkisyolé Alarcón
BA
D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001681:Biological Assay; D001771:Blood Banks; D001782:Blood Donors; D014355:Chagas Disease / Q000097:blood* / Q000175:diagnosis / Q000517:prevention & control; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008403:Mass Screening; D011237:Predictive Value of Tests; D012239:Rhodnius / Q000469:parasitology; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000302:isolation & purification; D014687:Venezuela / Q000453:epidemiology
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G