Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans.
Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)(5) primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)(5)-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)(5) primer to be a fast and reliable method for identification of S. mutans.
Svec
Pavel
P
Czech Collection of Microorganisms, Institute of Experimental Biology, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic. mpavel@sci.muni.cz
Nováková
Dana
D
Zácková
Lenka
L
Kukletová
Martina
M
Sedlácek
Ivo
I
D015373:Bacterial Typing Techniques / Q000379:methods*; D002648:Child; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D013290:Streptococcal Infections / Q000382:microbiology*; D013295:Streptococcus mutans / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial