title: Antigen persistence is required for dendritic cell licensing and CD8+ T cell cross-priming.
abstract: It has been demonstrated that CD4(+) T cells require Ag persistence to achieve effective priming, whereas CD8(+) T cells are on "autopilot" after only a brief exposure. This finding presents a disturbing conundrum as it does not account for situations in which CD8(+) T cells require CD4(+) T cell help. We used a physiologic in vivo model to study the requirement of Ag persistence for the cross-priming of minor histocompatibility Ag-specific CD8(+) T cells. We report inefficient cross-priming in situations in which male cells are rapidly cleared. Strikingly, the failure to achieve robust CD8(+) T cell activation is not due to a problem with cross-presentation. In fact, by providing "extra help" in the form of dendritic cells (DCs) loaded with MHC class II peptide, it was possible to achieve robust activation of CD8(+) T cells. Our data suggest that the "licensing" of cross-presenting DCs does not occur during their initial encounter with CD4(+) T cells, thus accounting for the requirement for Ag persistence and suggesting that DCs make multiple interactions with CD8(+) T cells during the priming phase. These findings imply that long-lived Ag is critical for efficient vaccination protocols in which the CD8(+) T cell response is helper-dependent.
authors: Immunology Department, Institut Pasteur, Paris France.
authors: Hélène
authors: H
authors: Jusforgues-Saklani
authors: Martin
authors: M
authors: Uhl
authors: Nathalie
authors: N
authors: Blachère
authors: Fabrice
authors: F
authors: Lemaître
authors: Olivier
authors: O
authors: Lantz
authors: Philippe
authors: P
authors: Bousso
authors: Deborah
authors: D
authors: Braun
authors: James J
authors: JJ
authors: Moon
authors: Matthew L
authors: ML
authors: Albert
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D045142:Cross-Priming / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015778:Minor Histocompatibility Antigens / Q000276:immunology; D014612:Vaccines / Q000276:immunology
chemical_list: D000941:Antigens; D000949:Histocompatibility Antigens Class II; D015778:Minor Histocompatibility Antigens; D014612:Vaccines