Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
Lysine acetylation and its regulatory enzymes are known to have pivotal roles in mammalian cellular physiology. However, the extent and function of this modification in prokaryotic cells remain largely unexplored, thereby presenting a hurdle to further functional study of this modification in prokaryotic systems. Here we report the first global screening of lysine acetylation, identifying 138 modification sites in 91 proteins from Escherichia coli. None of the proteins has been previously associated with this modification. Among the identified proteins are transcriptional regulators, as well as others with diverse functions. Interestingly, more than 70% of the acetylated proteins are metabolic enzymes and translation regulators, suggesting an intimate link of this modification to energy metabolism. The new dataset suggests that lysine acetylation could be abundant in prokaryotic cells. In addition, these results also imply that functions of lysine acetylation beyond regulation of gene expression are evolutionarily conserved from bacteria to mammals. Furthermore, we demonstrate that bacterial lysine acetylation is regulated in response to stress stimuli.
Zhang
Junmei
J
Department of Biochemistry, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Sprung
Robert
R
Pei
Jimin
J
Tan
Xiaohong
X
Kim
Sungchan
S
Zhu
Heng
H
Liu
Chuan-Fa
CF
Grishin
Nick V
NV
Zhao
Yingming
Y
D000107:Acetylation; D020816:Amino Acid Motifs; D000818:Animals; D015153:Blotting, Western; D002952:Citric Acid Cycle; D019295:Computational Biology; D017124:Conserved Sequence; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D019143:Evolution, Molecular; D006019:Glycolysis; D008239:Lysine / Q000378:metabolism*; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D040901:Proteomics; D013312:Stress, Physiological; D013379:Substrate Specificity; D014158:Transcription, Genetic
D029968:Escherichia coli Proteins; D008239:Lysine