title: [Determination of vitexin-rhamnoside in Beagle dog plasma and preliminary pharmacokinetics of Yixintong sustained release tablets].
abstract: OBJECTIVE
  To develop an HPLC method to determine vitexin-rhamnoside in plasma of Beagle dogs and study the pharmacokinetics and bioavailability of Yixintong sustained release tablets in Beagle dogs.
  
  
  METHODS
  A newly-developed HPLC method using C18 column and methanol-acetonitrile-tetrahydrogenfuran-0.5% acetic acid (1:1:19.4:78.6) as mobile phase was validated, and then was employed to determine vitexin-rhamnoside in plasma of Beagle dogs after oral administration of Yixintong sustained release tablets and general tablets. The main pharmacokinetic parameters were estimated by pharmacokinetic program 3p87. The non-compartmental pharmacokinetic parameters were also calculated on basis of the statistic moment theory.
  
  
  RESULTS
  The pharmacokinetic profiles of Yixintong sustained release tablets and the general tablets were fitted to a one-and two-compartment open model, respectively. The T1/2, Tmax, AUC0-infinity and MRT for Yixintong sustained release tablets were 5.22 h, 4.0 h, 6,792.75 ng x h x mL(-1) and 8.4 h, respectively, compared with 8.94 h, 1.0 h, 5,880.4 ng x h x mL(-1) and 6.1 h for the general tablets. The relative bioavailability of the Yixintong sustained release tablets was 115.5% in Beagle dogs.
  
  
  CONCLUSIONS
  The sustained-release characteristic of Yixintong sustained release tablets were confirmed by pharmacokinetic study.
authors: Department of Pharmaceutics, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
authors: Lu
authors: L
authors: Qu
authors: Hang-Sheng
authors: HS
authors: Zheng
authors: Nian-Ping
authors: NP
authors: Feng
authors: Song-Ming
authors: SM
authors: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D047310:Apigenin / Q000737:chemistry*; D001682:Biological Availability; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D008297:Male; D010949:Plasma / Q000737:chemistry* / Q000187:drug effects; D013607:Tablets / Q000008:administration & dosage / Q000493:pharmacokinetics
chemical_list: D004365:Drugs, Chinese Herbal; D013607:Tablets; C021329:vitexin rhamnoside; D047310:Apigenin