title: [Cryopreservation strengthens procoagulative activities of platelets].
abstract: The aim of this study was to explore the potential relationship between the enhancement of instant hemostatic function in vivo of cryopreserved platelets and its procoagulative related molecule activities. The ability of platelet binding factor V density of GPIb-IX-V (CD42a) at platelet member surface were detected by flow cytometry, the clotting time induced by activated platelets were evaluated by coagulometer and platelet count, MPV and PDW were measured by hemocytometer before and after fresh platelets were cryopreserved. The results showed that the clotting time induced by activated cryopreserved platelets decreased by 43.9%, even quicker than that induced by fresh platelets; the fluorescence intensity of cryopreserved platelet binding factor V increased by 117%, more than that of fresh platelets binding factor V; the GPIb-IX-V (CD42a) density at cryopreserved platelet membrane surface increased by 32%, higher than that at fresh platelet surface. It is concluded that the enhancement of instant hemostatic function in vivo of cryopreserved platelet may be related to higher expression of procoagulative molecules or to their enhanced activity and rapid hemostatic effect.
authors: Department of Blood Transfusion, The First Affiliated Hospital of PLA General Hospital, Beijing 100037, China.
authors: Xi-Lin
authors: XL
authors: Ouyang
authors: Dan
authors: D
authors: Zhou
authors: Jing-Hui
authors: JH
authors: Wu
authors: Li-Hua
authors: LH
authors: Wang
authors: Jun
authors: J
authors: Hao
authors: Jing-Han
authors: JH
authors: Liu
mesh_terms: D001777:Blood Coagulation / Q000502:physiology; D001779:Blood Coagulation Factors / Q000378:metabolism; D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000032:analysis
chemical_list: D001779:Blood Coagulation Factors; D019038:Platelet Glycoprotein GPIb-IX Complex; C037685:platelet factor V