title: Epigenetic reprogramming by adenovirus e1a.
abstract: Adenovirus e1a induces quiescent human cells to replicate. We found that e1a causes global relocalization of the RB (retinoblastoma) proteins (RB, p130, and p107) and p300/CBP histone acetyltransferases on promoters, the effect of which is to restrict the acetylation of histone 3 lysine-18 (H3K18ac) to a limited set of genes, thereby stimulating cell cycling and inhibiting antiviral responses and cellular differentiation. Soon after expression, e1a binds transiently to promoters of cell cycle and growth genes, causing enrichment of p300/CBP, PCAF (p300/CBP-associated factor), and H3K18ac; depletion of RB proteins; and transcriptional activation. e1a also associates transiently with promoters of antiviral genes, causing enrichment for RB, p130, and H4K16ac; increased nucleosome density; and transcriptional repression. At later times, e1a and p107 bind mainly to promoters of development and differentiation genes, repressing transcription. The temporal order of e1a binding requires its interactions with p300/CBP and RB proteins. Our data uncover a defined epigenetic reprogramming leading to cellular transformation.
authors: Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.
authors: Roberto
authors: R
authors: Ferrari
authors: Matteo
authors: M
authors: Pellegrini
authors: Gregory A
authors: GA
authors: Horwitz
authors: Wei
authors: W
authors: Xie
authors: Arnold J
authors: AJ
authors: Berk
authors: Siavash K
authors: SK
authors: Kurdistani
mesh_terms: D017500:Adenovirus E1A Proteins / Q000235:genetics / Q000378:metabolism*; D000260:Adenoviruses, Human / Q000235:genetics / Q000378:metabolism*; D050882:CREB-Binding Protein / Q000378:metabolism; D002472:Cell Transformation, Viral; D050719:Crk-Associated Substrate Protein / Q000235:genetics / Q000378:metabolism; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism; D006801:Humans; D009154:Mutation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism; D050720:Retinoblastoma-Like Protein p107 / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D050880:p300-CBP Transcription Factors / Q000378:metabolism; D000099224:p300-CBP-Associated Factor
chemical_list: D017500:Adenovirus E1A Proteins; D050882:CREB-Binding Protein; D050719:Crk-Associated Substrate Protein; D006657:Histones; D012333:RNA, Messenger; D016160:Retinoblastoma Protein; D050720:Retinoblastoma-Like Protein p107; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C493841:BCAR1 protein, human; C493844:RBL1 protein, human; C494363:CREBBP protein, human