title: Expression of stathmin, a microtubule regulatory protein, is associated with the migration and differentiation of cultured early trophoblasts.
abstract: BACKGROUND
  The microtubule-destabilizing protein stathmin is expressed by the villous cytotrophoblasts and invasive extravillous trophoblasts (EVTs) in the first-trimester human placenta. Here, we evaluated the significance of stathmin expression in terms of the functions of trophoblasts.
  
  
  METHODS
  We employed two choriocarcinoma cell lines (BeWo and JEG-3), an EVT cell line (HTR-8/SVneo) and isolated first-trimester trophoblast cells. The effects of small-interfering (si) RNA-mediated stathmin knockdown on trophoblast proliferation and migration were measured by WST-1 and Transwell assays, respectively. Trophoblast differentiation was induced by dibutyryl (db)-cAMP treatment and evaluated by measuring human chorionic gonadotrophin beta (hCGbeta) and syncytin expression and cell fusion. We examined the effect of knockdown and induced stathmin expression on db-cAMP-induced differentiation.
  
  
  RESULTS
  siRNA-induced silencing of stathmin expression had a marked inhibitory effect on BeWo, JEG-3 and HTR-8/SVneo cell migration and also suppressed their proliferation, albeit to a lesser extent. db-cAMP-enhanced hCGbeta and syncytin expression and cell fusion in BeWo cells was inhibited by stathmin knockdown. However, induced expression of stathmin reversed the hCGbeta and syncytin expression and cell fusion in the Tet-On BeWo cells. Suppression of stathmin expression also inhibited the migration of and hCGbeta production by first-trimester trophoblasts.
  
  
  CONCLUSIONS
  Stathmin expression may be closely associated with early trophoblast migration and differentiation into syncytiotrophoblasts during placentation.
authors: Department of Endocrine Pharmacology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.
authors: Mikihiro
authors: M
authors: Yoshie
authors: Hideaki
authors: H
authors: Kashima
authors: Toshio
authors: T
authors: Bessho
authors: Makoto
authors: M
authors: Takeichi
authors: Keiichi
authors: K
authors: Isaka
authors: Kazuhiro
authors: K
authors: Tamura
mesh_terms: D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D006801:Humans; D011247:Pregnancy; D050777:Stathmin / Q000096:biosynthesis*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism* / Q000502:physiology
chemical_list: C493995:STMN1 protein, human; D050777:Stathmin