Two-year study evaluating the potential loss of methicillin resistance in a methicillin-resistant Staphylococcus aureus culture collection.
A reported loss of mecA prompted us to monitor 360 cryostocked methicillin-resistant Staphylococcus aureus strains for stability. Concurrently, 14 well-characterized strains were stored in a Microbank preservation system and subjected to multiple freeze-thaw events. There were no significant declines in the methicillin-resistant populations with either method over a two-year period.
Veguilla
William
W
Center for Biological Defense, College of Public Health, University of South Florida, 3602 Spectrum Blvd., Tampa, FL 33612, USA. wveguill@health.usf.edu
Peak
K Kealy
KK
Luna
Vicki A
VA
Roberts
Jill C
JC
Davis
Carisa R
CR
Cannons
Andrew C
AC
Amuso
Philip
P
Cattani
Jacqueline
J
D001426:Bacterial Proteins / Q000235:genetics*; D015169:Colony Count, Microbial; D005615:Freezing; D017353:Gene Deletion; D016106:Methicillin Resistance; D046915:Penicillin-Binding Proteins; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*
D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus