title: A latex agglutination assay for specific detection of Panton-Valentine leukocidin.
abstract: Panton-Valentine leukocidin (PVL) is produced by some isolates of Staphylococcus aureus, and has been associated with the high pathogenic potential of these strains. To rapidly detect the toxin producer strains, we developed a reverse passive latex agglutination (RPLA) reaction assay specific for PVL. By testing 64 S. aureus strains, the assay could detect the 35 pvl-gene-positive strains with 100% specificity and sensitivity. Furthermore, the assay revealed an extensive variation in the amount of PVL produced by the pvl-positive strains.
authors: Bacterial Diagnostics Production Department, Denka Seiken Co. Ltd., Gosen, Niigata, Japan.
authors: Kanenari
authors: K
authors: Oishi
authors: Tadashi
authors: T
authors: Baba
authors: Yasuo
authors: Y
authors: Nakatomi
authors: Teruyo
authors: T
authors: Ito
authors: Keiichi
authors: K
authors: Hiramatsu
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D005098:Exotoxins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D007956:Leukocidins / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin