[Authentication of Pinellia pedatisecta and their adulterants by AS-PCR].
OBJECTIVE
To establish a molecular method for the authentication of Pinellia pedatisecta and its adulterants.


METHODS
DNA sequences of some species from P. tenore, Typhonium and Arisaema were downloaded from GenBank, the sequences were aligned using DNAMAN. Allele-specific primers for P. pedatisecta and P. tenore were designed according to their SNPs in rpl 20 sequence. The designed primers were used to amplify 10 samples of P. pedatisecta, P. ternata and T. flagelliforme.


RESULTS
A 351 bp band was amplified from P. pedatisecta but not form P. ternata and T. flagelliforme by primer Pprpl149F and Pprpl484R. A 630 bp band was amplified from P. ternate and P. pedatisecta but not from T. flagelliforme by primer Ptrpl94F and Ptrpl699R.


CONCLUSIONS
AS-PCR has the advantages of highly specific and good reproducibility, by which P. pedatisecta can be identified from part of its adulterants quickly. It is a potential method to be used in the molecular identification of other materia medica.
Cui
Guang-Hong
GH
Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700, China.
Tang
Xiao-Jing
XJ
Huang
Lu-Qi
LQ
Qian
Zhong-Zhi
ZZ
D000483:Alleles; D002681:China; D003257:Consumer Product Safety; D017931:DNA Primers / Q000235:genetics; D030021:Pinellia / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D011786:Quality Control
D017931:DNA Primers