Embryonic stem cells derived from C57BL/6J and C57BL/6N mice.
Mouse embryonic stem (ES) cells with the C57BL/6 genetic background allow the generation of knockout mice without the need to backcross to C57BL/6. However, C57BL/6 ES cells whose pluripotency after homologous recombination has been confirmed are not yet available from public cell banks. To facilitate the use of ES cells derived from C57BL/6 sublines in both biologic and medical research, we demonstrated that the use of knockout serum replacement as a medium supplement and 8-cell blastomeres as recipient embryos allowed establishment of ES cells and production of germline chimeric mice, respectively. Under effective conditions, a large number of ES cell lines were established from C57BL/6J and C57BL/6N blastocysts. The majority of ES cells in many cell lines obtained from both strains showed a normal chromosome number. Germline chimeric mice were generated from C57BL/6J and C57BL/6N ES cells. Finally, the ES cell line B6J-S1UTR, derived from C57BL/6J, was used for successful production of gene knockout mice. C57BL/6J ES (B6J-S1UTR and B6J-23UTR) and C57BL/6N ES (B6N-22UTR) cells are available from the cell bank of the BioResource Center at RIKEN Tsukuba Institute (http://www.brc.riken.jp/lab/cell/english/).
Tanimoto
Yoko
Y
Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.
Iijima
Saori
S
Hasegawa
Yoshikazu
Y
Suzuki
Yuko
Y
Daitoku
Yoko
Y
Mizuno
Seiya
S
Ishige
Taichiro
T
Kudo
Takashi
T
Takahashi
Satoru
S
Kunita
Satoshi
S
Sugiyama
Fumihiro
F
Yagami
Ken-ichi
K
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D048430:Cell Shape; D002478:Cells, Cultured; D002678:Chimera / Q000235:genetics; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D018390:Gene Targeting; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011995:Recombination, Genetic
D003470:Culture Media