Evolving trends in the dereplication of natural product extracts: new methodology for rapid, small-scale investigation of natural product extracts.
The use of an HPLC bioactivity profiling/microtiter plate technique in conjunction with capillary probe NMR instrumentation and access to appropriate databases effectively short-circuits conventional dereplication procedures, necessarily based on multimilligram extracts, to a single, more rapid submilligram operation. This approach to dereplication is illustrated using fungal or bacterial extracts that contain known compounds. In each case the dereplication steps were carried out on microgram quantities of extract and demonstrate the discriminating power of (1)H NMR spectroscopy as a definitive dereplication tool.
Lang
Gerhard
G
Department of Chemistry, University of Canterbury, Private Bag 4800, Christchurch, New Zealand.
Mayhudin
Nor Ainy
NA
Mitova
Maya I
MI
Sun
Lin
L
van der Sar
Sonia
S
Blunt
John W
JW
Cole
Anthony L J
AL
Ellis
Gill
G
Laatsch
Hartmut
H
Munro
Murray H G
MH
D001688:Biological Products / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques / Q000191:economics / Q000379:methods*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry*
D001688:Biological Products; D010936:Plant Extracts