title: In vitro transient expression system of latex C-serum was used for analysis of hevein promoter in response to abscisic acid in Hevea brasiliensis.
abstract: Hevein has been found to be an essential element in coagulation of rubber particles in latex of rubber trees. In a previous study, we cloned a 1,241-bp fragment of a 5' upstream region of the hevein gene by genome walking. This fragment was analyzed by a 5' end nested deletion method in the present study, fused with a uidA (gus) gene to produce a series of tested constructs, which were transferred into C-serum of latex and the Gus activities were detected. Results showed that the fragment from -749 to -292 was sufficient for expression of gus gene in latex, and the fragment from -292 to -168 was crucial in response to abscisic acid inducement. In a transient transgenic test of rubber leaf with particle bombardment, construct Hev749 conferred gus-specific expression in veins, in which the latex tubes mainly distributed. This implies that the fragment from -749 to -292 was laticiferous-specific.
authors: Institute of Tropical Bioscience and Biotechnology, Chinese Agricultural Academy for Tropical Crops, Haikou 571101, China.
authors: Xiao-Wen
authors: XW
authors: Fei
authors: Xiao-Dong
authors: XD
authors: Deng
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D020029:Base Pairing / Q000187:drug effects; D002498:Centrifugation; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D028482:Hevea / Q000187:drug effects* / Q000235:genetics; D007840:Latex / Q000378:metabolism*; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D037121:Plant Lectins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017384:Sequence Deletion
chemical_list: D023181:Antimicrobial Cationic Peptides; D007840:Latex; D037121:Plant Lectins; C045875:hevein; D000040:Abscisic Acid; D005966:Glucuronidase