Probing ras effector interactions on nanoparticle supported lipid bilayers.
Many biological processes take place in close proximity to lipid membranes. For a detailed understanding of the underlying mechanisms, tools are needed for the quantitative characterization of such biomolecular interactions. In this work, we describe the development of methods addressing the dynamics and affinities of protein complexes attached to an artificial membrane system. A semisynthetic approach provides the Ras protein with palmitoyl anchors, which allow stable membrane insertion, as a paradigm for membrane associated proteins that interact with multiple effectors. An artificial membrane system is constituted by nanoparticles covered with a lipid bilayer. Such a stable suspension allows for the characterization of the interaction between membrane-bound Ras and effector proteins using conventional fluorescence-based methods.
Filchtinski
Daniel
D
Physikalische Chemie 1, Ruhr-Universität-Bochum, Fakultät für Chemie and Biochemie, Universitätsstr. 150, 44780 Bochum, Germany.
Bee
Christine
C
Savopol
Tudor
T
Engelhard
Martin
M
Becker
Christian F W
CF
Herrmann
Christian
C
D015374:Biosensing Techniques / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D053758:Nanoparticles / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods; D018631:ras Proteins / Q000737:chemistry / Q000378:metabolism*
D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010715:Phosphatidylglycerols; D018631:ras Proteins