[Determination of monoethylglycinexylidide concentration in serum using solid phase extraction and capillary gas chromatography-mass spectrometry].
A novel method for the determination of monoethylglycinexylidide (MEGX) (lidocaine metabolin) in serum using solid phase extraction (SPE) and capillary gas chromatography-mass spectrometry (GC-MS) was established. The serum sample was extracted with a CN-SPE column. An HP-5MS capillary column (15 m x 0.25 mm x 0.1 microm) was used. The initial temperature of the column was set at 100 degrees C, held for 1 min, then raised to 200 degrees C at 40 degrees C/min, and held at 200 degrees C for 0.5 min. The sample size was 2 microL, and the split ratio was set at 1 : 1. The carrier gas was high purity helium with a flow rate of 1.0 mL/min. The monitoring ions for the determination were m/z 58 for MEGX and m/z 86 for procaine (internal standard). The calibration curve of MEGX had good linear relationship in the range of 1.562 - 25 ng/mL ( r = 0.998 1). The limit of detection was 0.5 ng/mL. The extraction recovery ranged from 80.1% to 85.7%. The method advanced the quantitative analysis of MEGX in serum by combining rapid and efficient SPE with specific and sensitive quantitation by GC-MS.
Wang
Zhenxia
Z
General Surgery Department, First Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010050, China. wzhenxia@163.com
Zhang
Ruiming
R
Cao
Wei
W
Ouyang
Xiaohui
X
Zhao
Haiping
H
Dong
Peide
P
Meng
Xingkai
X
D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008012:Lidocaine / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification; D051381:Rats; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013997:Time Factors
D008012:Lidocaine; C001173:monoethylglycinexylidide