Subacute toxicity of polychlorinated naphthalenes and their effect on cytochrome P-450.
The aim of the study was to investigate the subacute toxicity of a polychlorinated naphthalene (PCN) mixture and its effect on cytochrome P-450 levels in rats. The animals were administered PCNs intragastrically in repeated daily doses of 1, 10, and 100 mg/kg. The animals were dissected after 7, 14, or 21 doses. Doses of 10 and 100 mg/kg induced a significant decrease in the body weight at all time points of the experiment compared with the control group. The exposure to PCNs increased both the level of total cytochrome P-450 and the activity of CYP 1A at the same time points. In the groups of rats given PCNs in doses of 10 and 100 mg/kg, an evident dose- and time-dependent increase in malondialdehyde (MDA) level was observed throughout the experiment. The correlation between the increased MDA and decreased glutathione (GSH) levels in the liver was also observed.
Kilanowicz
Anna
A
Faculty of Pharmacy, Department of Toxicology, Medical University, Muszynskiego 1, 90-151 Lodz, Poland. akilanowicz@pharm.am.lodz.pl
Skrzypinska-Gawrysiak
Malgorzata
M
Sapota
Andrzej
A
Galoch
Aneta
A
Daragó
Adam
A
D000818:Animals; D001835:Body Weight / Q000187:drug effects* / Q000502:physiology; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D006843:Hydrocarbons, Chlorinated / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009281:Naphthalenes / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D006843:Hydrocarbons, Chlorinated; D009281:Naphthalenes; D008315:Malondialdehyde; D003577:Cytochrome P-450 Enzyme System; D005978:Glutathione