[Optimization of plantaricin production by Lactobacillus plantarum ZJ316].
OBJECTIVE
To enhance the production of plantaricin by Lactobacillus plantarum ZJ316 isolated from infant feces.


METHODS
We analyzed fermentation parameters influencing cell growth and plantaricin production with different medium composition and under different cultivation conditions.


RESULTS
MRS (DE MAN, ROGOSA, SHARPE) medium was more suitable for producing bacteriocin than other media. The maximum plantaricin production was obtained in modified MRS medium containing 10 g/L maltose and 10 g/L glucose, 10 g/L yeast extract, 10 g/L tryptone and 2g/L tri-ammonium citrate, 1 mL/L Tween80, 6 g/L K2HPO4.3H2O, 5 g/L sodium acetate, 0.2 g/L magnesium sulfate and 0.05 g/L manganese sulfate. The optimal initial pH and temperature for plantaricin production were 6.5 and 30 degrees C for 24 h.


CONCLUSIONS
After optimizing, the production of plantaricin was increased 2.3-fold using the optimized medium, compared with in the basic MRS medium.
Li
Jingliang
J
College of Food & Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, China. lijingliang000@msn.com
Song
Dafeng
D
Gu
Qing
Q
D001430:Bacteriocins / Q000096:biosynthesis*; D001431:Bacteriological Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009584:Nitrogen / Q000737:chemistry / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology; D013696:Temperature
D001430:Bacteriocins; D003470:Culture Media; D010710:Phosphates; D017680:Potassium Compounds; D002244:Carbon; C013216:potassium phosphate; D009584:Nitrogen