[Separation and purification process of total flavones and total tannins from Apocynum venetum leaves with macroreticular resin].
OBJECTIVE
To investigate the purification process of total flavones and total tannins from Apocynum venetum leaves with macroreticular resin.


METHODS
The static capacity absorption, dynamic absorption ratio and dynamic elution ratio of different types of macroreticular resins were studied and compared in order to find the best one among the eight macroreticular resins, and the technical process of the type of HPD-400 type macroreticular resin was studied in detail.


RESULTS
The type of HPD-400 type macroreticular resin showed the best comprehensive absorption property with the following technological conditions: the current velocity was 1 BV x h(-1), the volume of distilled water was 2 BV, the eluting reagent was 60% ethanol, and the volume of 60% ethanol was 3 BV.


CONCLUSIONS
The purity of total flavones and total tannins in the product is up to 80% after purified with HPD-400 macroreticular resin. Therefore, this purification process is feasible.
Zhang
Su-Qiong
SQ
School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, China.
Hou
Jin-Jun
JJ
Li
Qing-Shan
QS
D000327:Adsorption; D030010:Apocynum / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification*
D004365:Drugs, Chinese Herbal; D047309:Flavones; D012117:Resins, Synthetic; D013634:Tannins