title: In vitro and in vivo antioxidant and anti-inflammatory capacities of an antioxidant-rich fruit and berry juice blend. Results of a pilot and randomized, double-blinded, placebo-controlled, crossover study.
abstract: This study investigated the in vitro and in vivo antioxidant and anti-inflammatory properties of a juice blend (JB), MonaVie Active, containing a mixture of fruits and berries with known antioxidant activity, including acai, a palm fruit, as the predominant ingredient. The phytochemical antioxidants in the JB are primarily in the form of anthocyanins, predominantly cyanidin 3-rutoside, cyanidin 3-diglycoside, and cyanidin 3-glucoside. The cell-based antioxidant protection of erythrocytes (CAP-e) assay demonstrated that antioxidants in the JB penetrated and protected cells from oxidative damage ( p < 0.001), whereas polymorphonuclear cells showed reduced formation of reactive oxygen species ( p < 0.003) and reduced migration toward three different pro-inflammatory chemoattractants: fmlp ( p < 0.001), leukotriene B4 ( p < 0.05), and IL-8 ( p < 0.03). A randomized, double-blinded, placebo-controlled, crossover trial with 12 healthy subjects examined the JB's antioxidant activity in vivo. Blood samples at baseline, 1 h, and 2 h following consumption of the JB or placebo were tested for antioxidant capacity using several antioxidant assays and the TBARS assay, a measure of lipid peroxidation. A within subject comparison showed an increase in serum antioxidants at 1 h ( p < 0.03) and 2 h ( p < 0.015), as well as inhibition of lipid peroxidation at 2 h ( p < 0.01) postconsumption.
authors: Holger NIS Inc., 601 13th Avenue N.E., Calgary, Alberta, Canada T2E 1C7. gitte@holgernis.com
authors: Gitte S
authors: GS
authors: Jensen
authors: Xianli
authors: X
authors: Wu
authors: Kelly M
authors: KM
authors: Patterson
authors: Janelle
authors: J
authors: Barnes
authors: Steve G
authors: SG
authors: Carter
authors: Larry
authors: L
authors: Scherwitz
authors: Robert
authors: R
authors: Beaman
authors: John R
authors: JR
authors: Endres
authors: Alexander G
authors: AG
authors: Schauss
mesh_terms: D000328:Adult; D000872:Anthocyanins / Q000008:administration & dosage; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D000975:Antioxidants / Q000008:administration & dosage*; D001628:Beverages; D018592:Cross-Over Studies; D004311:Double-Blind Method; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects; D010865:Pilot Projects; D010919:Placebos; D017382:Reactive Oxygen Species / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000872:Anthocyanins; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010919:Placebos; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances