Stabilization of the pertussis toxin secretion apparatus by the C terminus of PtlD.
Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 72 amino acids of PtlD (amino acids 392 to 463) was sufficient for maintaining the stability of PtlE, PtlF, and PtlH, although this region was not sufficient to support secretion of the toxin. Further analysis demonstrated that a stretch of 10 amino acids at the C-terminal end of PtlD (amino acids 425 to 434) contributes to transporter stability.
Verma
Anita
A
Laboratory of Respiratory and Special Pathogens, Food and Drug Administration, CBER, FDA HFM-434, Building 29, Room 130, 8800 Rockville Pike, Bethesda, MD 20892, USA.
Cheung
Anissa M
AM
Burns
Drusilla L
DL
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001886:Bordetella pertussis / Q000235:genetics / Q000378:metabolism*; D005816:Genetic Complementation Test; D015151:Immunoblotting; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D009154:Mutation; D037342:Pertussis Toxin / Q000378:metabolism*
D001426:Bacterial Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C109410:PtlH protein, Bordetella pertussis; C094736:ptlF protein, Bordetella pertussis; D037342:Pertussis Toxin