Injecting skin fibroblasts into muscles to express human clotting factor IX cDNA.
Primary rabbit skin fibroblasts (RSF) containing lacZ reporter gene or human clotting factor IX (hFIX) cDNA could long-termly exist and be expressed after being implanted in rabbit muscles. Among them, the beta-glactosidase expression lasted at least 3 months, and hFIX expression lasted 1 month. These results will be helpful to organically combine the advantages of both skin fibroblasts and muscle, and provide a new way for somatic gene therapy of hemophilia B and other genetic diseases.
Wang
H
H
Institute of Genetics, Fudan University, 200433, Shanghai, China.
Yang
X
X
Xing
Y
Y
Lu
D
D
Qiu
X
X
Xue
J
J