Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis.
Cytoplasmic Cu/Zn superoxide dismutase (SOD1) is an antioxidant enzyme that converts superoxide to hydrogen peroxide in cells. Its spatial distribution matches that of superoxide production, allowing it to protect cells from oxidative stress. SOD1 deficiencies result in embryonic lethality and a wide range of pathologies in mice, but little is known about normal SOD1 protein expression in developing embryos. In this study, the expression pattern of SOD1 was investigated in post-implantation mouse embryos and extraembryonic tissues, including placenta, using Western blotting and immunohistochemical analyses. SOD1 was detected in embryos and extraembryonic tissues from embryonic day (ED) 8.5 to 18.5. The signal in embryos was observed at the lowest level on ED 9.5-11.5, and the highest level on ED 17.5-18.5, while levels remained constant in the surrounding extraembryonic tissues during all developmental stages examined. Immunohistochemical analysis of SOD1 expression on ED 13.5-18.5 revealed its ubiquitous distribution throughout developing organs. In particular, high levels of SOD1 expression were observed in the ependymal epithelium of the choroid plexus, ganglia, sensory cells of the olfactory and vestibulocochlear epithelia, blood cells and vessels, hepatocytes and hematopoietic cells of the liver, lymph nodes, osteogenic tissues, and skin. Thus, SOD1 is highly expressed at late stages of embryonic development in a cell- and tissue-specific manner, and can function as an important antioxidant enzyme during organogenesis in mouse embryos.
Yon
Jung-Min
JM
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.
Baek
In-Jeoung
IJ
Lee
Se-Ra
SR
Kim
Mi-Ra
MR
Lee
Beom Jun
BJ
Yun
Young Won
YW
Nam
Sang-Yoon
SY
D000818:Animals; D002540:Cerebral Cortex / Q000196:embryology / Q000201:enzymology; D003307:Copulation; D003593:Cytoplasm / Q000201:enzymology*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology / Q000201:enzymology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D038081:Organogenesis / Q000502:physiology*; D011247:Pregnancy; D013270:Stomach / Q000196:embryology / Q000201:enzymology; D013482:Superoxide Dismutase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
D013482:Superoxide Dismutase