title: Expression of chloride intracellular channel protein 1 (CLIC1) and tumor protein D52 (TPD52) as potential biomarkers for colorectal cancer.
abstract: OBJECTIVE
  Unequivocal biomarkers are needed to predict susceptibility and progression of colorectal cancer.
  
  
  METHODS
  Paired samples of tumor and normal tissue from six patients with colorectal cancer of different localization, pTNM stage and grade were employed in the present study. MS analysis was used to identify differentially regulated proteins after 2-DE separation and densitometric analysis.
  
  
  RESULTS
  Densitometric analysis revealed differential abundance of 55 spots in tumor as compared to normal tissues. Thirty nine out of 55 spots were unambiguously identified by MS representing 32 different proteins. CLIC1, TPD52 and FABPL were consistently overexpressed (>3-fold, P<0.05) in all tumor tissue samples, while TPM1, TPM2, TPM3, TAGL and MLRN were consistently down-regulated (>3-fold, P<0.05) compared to normal tissue.
  
  
  CONCLUSIONS
  CLIC1 and TPD52 were significantly (P<0.05) up-regulated in all cases of colorectal cancer investigated, irrespective of localization, pTNM stage and grade of colon cancer highlighting their potential to serve as new biomarkers.
authors: Department of Medical Genetics, Medical University, Sofia, Bulgaria.
authors: Darinka Todorova
authors: DT
authors: Petrova
authors: Abdul R
authors: AR
authors: Asif
authors: Victor W
authors: VW
authors: Armstrong
authors: Ivanka
authors: I
authors: Dimova
authors: Svetoslav
authors: S
authors: Toshev
authors: Nikolay
authors: N
authors: Yaramov
authors: Michael
authors: M
authors: Oellerich
authors: Draga
authors: D
authors: Toncheva
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018118:Chloride Channels / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D003710:Demography; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor; C501966:CLIC1 protein, human; D018118:Chloride Channels; D009363:Neoplasm Proteins; C114751:TPD52 protein, human