title: Bacterial cell wall products increases stabilization of HIF-1 alpha in an oligodendrocyte cell line preconditioned by cobalt chloride or desferrioxamine.
abstract: We examined the effect of lipopolysaccharide (LPS) or lipotechoic acid (LTA) on the regulation of hypoxia inducible factor (HIF-1) alpha on the MO3.13 cells, a human oligodendroglial cell line. Our study shows that MO3.13 cells express the toll like receptors (TLR's) but do not increase cellular levels of HIF-1 alpha following exposure to bacterial cell wall products. When MO3.13 cells were preconditioned by desferrioxamine (DFO) or cobalt chloride (CoCl(2)) and then treated with either LPS or LTA, HIF-1 alpha levels were higher than that induced by DFO or CoCl(2) alone. The increase in HIF-1 alpha was due to increased protein stability that was mediated by activation of the ERK-MAP kinase pathway.
authors: Department of Neurology, Multiple Sclerosis Research Center, Vanderbilt University Medical Center, Nashville, Tennessee 37212, USA.
authors: Song-yi
authors: SY
authors: Yao
authors: Mohammed
authors: M
authors: Soutto
authors: Subramaniam
authors: S
authors: Sriram
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D001419:Bacteria / Q000166:cytology; D002461:Cell Line, Transformed; D002473:Cell Wall / Q000737:chemistry; D003035:Cobalt / Q000494:pharmacology*; D003676:Deferoxamine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009836:Oligodendroglia / Q000187:drug effects*; D017262:Siderophores / Q000494:pharmacology*; D013682:Teichoic Acids / Q000494:pharmacology; D013997:Time Factors; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism
chemical_list: D016587:Antimutagenic Agents; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008070:Lipopolysaccharides; D017262:Siderophores; D013682:Teichoic Acids; D051193:Toll-Like Receptors; D003035:Cobalt; C009900:lipoteichoic acid; C018021:cobaltous chloride; D003676:Deferoxamine