[Hemostatic effect of hemocoagulase agkistrodon and its mechanism].
This study was aimed to investigate the hemostatic effects of hemocoagulase agkistrodon (HCA) and its mechanism. The procoagulative and hemostatic effects of HCA were evaluated by using rabbit blood coagulatin time and mouse tail bleeding time; the mechanisms of HCA hemostatic effect were analyzed by using rabbit blood clot lysis and fibrinogen lysis. The results showed that HCA shortened the rabbit blood coagulation time and the mouse tail bleeding time significantly. The effects are nearly similar to that of positive control (reptilase). HCA also induced rabbit blood clot lysis and directly hydrolysed the alpha-chain of fibrinogen. It is concluded that HCA exert its hemostatic effects by hydrolysing the alpha-chain of fibrinogen, but it is not able to induce production of XIII factor.
Lü
Hui-Min
HM
National Institute of Radiological Protection and Nuclear Safety, Chinese Center for Disease Control and Prevention, Beijing 100080, China.
Li
Chang-Ling
CL
Dong
Jin-Chan
JC
Zhang
Cui-Lan
CL
Zhou
Xiang-Yan
XY
D017836:Agkistrodon; D000818:Animals; D012103:Batroxobin / Q000302:isolation & purification / Q000494:pharmacology*; D001760:Bleeding Time; D001777:Blood Coagulation / Q000187:drug effects; D003435:Crotalid Venoms / Q000201:enzymology*; D005340:Fibrinogen / Q000378:metabolism; D006490:Hemostatics / Q000494:pharmacology*; D051379:Mice; D011817:Rabbits; D011897:Random Allocation
D003435:Crotalid Venoms; D006490:Hemostatics; D005340:Fibrinogen; D012103:Batroxobin