title: Dynamics of neutrophil migration in lymph nodes during infection.
abstract: Although the signals that control neutrophil migration from the blood to sites of infection have been well characterized, little is known about their migration patterns within lymph nodes or the strategies that neutrophils use to find their local sites of action. To address these questions, we used two-photon scanning-laser microscopy to examine neutrophil migration in intact lymph nodes during infection with an intracellular parasite, Toxoplasma gondii. We found that neutrophils formed both small, transient and large, persistent swarms via a coordinated migration pattern. We provided evidence that cooperative action of neutrophils and parasite egress from host cells could trigger swarm formation. Neutrophil swarm formation coincided in space and time with the removal of macrophages that line the subcapsular sinus of the lymph node. Our data provide insights into the cellular mechanisms underlying neutrophil swarming and suggest new roles for neutrophils in shaping immune responses.
authors: Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, CA 94720, USA.
authors: Tatyana
authors: T
authors: Chtanova
authors: Marie
authors: M
authors: Schaeffer
authors: Seong-Ji
authors: SJ
authors: Han
authors: Giel G
authors: GG
authors: van Dooren
authors: Marcelo
authors: M
authors: Nollmann
authors: Paul
authors: P
authors: Herzmark
authors: Shiao Wei
authors: SW
authors: Chan
authors: Harshita
authors: H
authors: Satija
authors: Kristin
authors: K
authors: Camfield
authors: Holly
authors: H
authors: Aaron
authors: Boris
authors: B
authors: Striepen
authors: Ellen A
authors: EA
authors: Robey
mesh_terms: D000818:Animals; D002465:Cell Movement; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology* / Q000469:parasitology; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000469:parasitology; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*