The onset of foreign gene transcription in nuclear-transferred embryos of fish.
The transcriptional onset ofhGH-transgene in fish was studied in the following three cases: the first is inMThGH-transgenic F(4) common carp (Cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic F(4) embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic F(4) tail-fin nuclei. RT-PCR results show that thehGH-transgene initiates its transcriptional activity from early-gastrula stage, the early blastula stage and even 16-cell stage in the first, second and third cases, respectively. It looks like that fish egg cytoplasm could just offer a very restricted reprogramming on transcriptional activity of specific gene in differentiated cell nuclei by nuclear transplantation.
Sun
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Y
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, 430072, Wuhan, China.
Chen
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S
Wang
Y
Y
Zhu
Z
Z