title: [Effect of coking oven emissions on level of serum oxidation-reduction and peripheral white blood cellular ras P21 and P53 in coke oven workers].
abstract: OBJECTIVE
  To explore the adverse effects of coking oven emissions (COE) on the serum oxidation-reduction and relevant genes in the exposed workers.
  
  
  METHODS
  Fifty-six coke oven workers and forty controls were investigated. Serum Malondialdehyde (MDA) levels and the activities of total superoxide dismutases (T-SOD) were measured by spectrophotometrical method. Immunohistochemical method was used to assess the P21 and P53 levels in peripheral white blood cells.
  
  
  RESULTS
  Compared with controls, the individuals exposed to COE had significantly increased levels of serum MDA [(5.30 +/- 2.29) nmol/mL, P < 0.01] and markedly decreased levels of T-SOD [(100.04 +/- 10.75) NU/mL]. Additionally, the median levels of P53 and P21 were markedly increased in the exposed individuals compared with the controls (21.4% and 23.2%, respectively, all P < 0.05).
  
  
  CONCLUSIONS
  The findings indicate that occupational exposure to COE causes the rise of serum oxidation-reduction MDA and the fall of T-SOD, and increasing expression levels of P21 and P53 proteins before the occurrence of apparent clinical symptoms.
authors: Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, China.
authors: Qiao
authors: Q
authors: Zhang
authors: Fang
authors: F
authors: Zhou
authors: Wu
authors: W
authors: Yao
authors: Yi-bo
authors: YB
authors: Zhao
authors: Zhi-yuan
authors: ZY
authors: Li
authors: Yu-bao
authors: YB
authors: Xu
authors: Yi-ming
authors: YM
authors: Wu
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects; D016022:Case-Control Studies; D003077:Coke / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects; D010084:Oxidation-Reduction; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000097:blood; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000395:Air Pollutants, Occupational; D003077:Coke; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)