Antioxidant effects of equol on bovine aortic endothelial cells.
This study is to examine the effects of equol on the H(2)O(2)-induced death of bovine aortic endothelial cells (bAECs) and the mechanism of its protective effects. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] assay showed that in the control group, cell survival rate decreased significantly, each in proportion to the duration of the H(2)O(2) stimulation (P<0.05), but, in the equol-pretreated group, such decrease was not statistically significant. After Hoechst 33342 staining, in the equol-pretreated group the number of cells with apoptotic morphology decreased significantly. Equol pretreatment effectively inhibited the H(2)O(2)-induced cell death by the reduction of intracellular ROS production (P<0.05). Incubation of bAECs with equol increased the expression of phospho-p38 MAPK and Bcl-2 after the H(2)O(2) exposure compared with their expression without the equol pretreatment. Furthermore, SB203580 inhibited phospho-p38 MAPK expression and increased apoptotic cell death. This study proves equol has a significant antioxidant effect on the bAECs that were exposed to H(2)O(2).
Chung
Jae-Eun
JE
Department of Obstetrics and Gynecology, College of Medicine, Wonkwang University Sanbon Medical Center of Korea, 1142 Sanbon-dong, Gunpo-si, Kyunggi-do 435-040, Republic of Korea.
Kim
Sue Yeon
SY
Jo
Hyun Hee
HH
Hwang
Seong Jin
SJ
Chae
Boah
B
Kwon
Dong Jin
DJ
Lew
Young Oak
YO
Lim
Young-Taik
YT
Kim
Jang Heub
JH
Kim
Eun Jung
EJ
Kim
Jin-Hong
JH
Kim
Mee-Ran
MR
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001011:Aorta / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002417:Cattle; D019610:Cytoprotection; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D060754:Equol; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology*; D048789:Phytoestrogens / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
C007610:4',7-dihydroxy-3,4-dihydroisoflavone; D000975:Antioxidants; D007093:Imidazoles; D007529:Isoflavones; D048789:Phytoestrogens; D019253:Proto-Oncogene Proteins c-bcl-2; D011725:Pyridines; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; D060754:Equol; D006861:Hydrogen Peroxide; D048051:p38 Mitogen-Activated Protein Kinases; C022616:thiazolyl blue; C093642:SB 203580