[Expression of rhodopsin for experimental myopia of form-deprivation and defocus in guinea pig].
OBJECTIVE
To investigate the rhodopsin expression in form-deprived and defocus myopia in guinea pig and study the relationship between the rhodopsin expression and experimental myopia.


METHODS
Fourty guinea pigs were randomized into the form-deprived group and the defocus group (n = 20). Guinea pigs in the form-deprived group wore a diffused (rigid glass-permeable contact lens (RGP) on one eye since one week after birth. Those in defocus group wore a -4 D RGP on one eye. The contralateral eyes were left as control. Refraction, axial length and depth of vitreous cavity were measured after 1 and 2 weeks respectively. Retina were dissected at 10-12 o'clock in the morning. The level of rhodopsin and its mRNA were observed through Western-blot and real-time PCR respectively.


RESULTS
There is no difference between form-deprived group, defocus group and control groups (except refraction in form-deprived group). One week later, there is no difference between the form-deprived group, the defocus group and the control groups (except refraction in form-deprived group). Two weeks later, eyes in the form-deprived group and the defocus group became myopic. Its axial length lengthened and depth of vitreous cavity appeared deep. The form-deprived groups showed an increased expression of rhodopsin and its mRNA compared to the control groups. There is no difference between the defocus group and the control groups.


CONCLUSIONS
Expression of rhodopsin might involve formation of form-deprived myopia, but has less influence on defocus myopia.
Hu
Min
M
Department of Ophthalmology, The Second People's Hospital of Yunnan Province Kunming 650021, China.
Zhong
Hua
H
Liu
Yongsong
Y
Liu
Rui
R
Chen
Chongda
C
Chu
Renyuan
R
D000818:Animals; D006168:Guinea Pigs; D009216:Myopia / Q000209:etiology / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism*; D012683:Sensory Deprivation
D012243:Rhodopsin