Targeting subcapsular antigens for prevention of Klebsiella pneumoniae infections.
Vaccination strategies against Klebsiella pneumoniae have largely focussed on the polysaccharide capsule. However, the large number and high prevalence of individual capsular serotypes limits the widespread applicability of capsule-based vaccines. This study establishes that immunization with purified LPS can protect mice against lethal challenge with K. pneumoniae, and that subcapsular antibodies directed against purified LPS can be used to treat and/or prevent experimental K. pneumoniae infection in mice. This approach offers potential for prophylaxis and/or therapy against drug-resistant strains of K. pneumoniae.
Clements
Abigail
A
Department of Microbiology & Immunology, The University of Melbourne, Parkville, Vic. 3010, Australia. abigailc@unimelb.edu.au
Jenney
Adam W
AW
Farn
Jacinta L
JL
Brown
Lorena E
LE
Deliyannis
Georgia
G
Hartland
Elizabeth L
EL
Pearse
Martin J
MJ
Maloney
Margaret B
MB
Wesselingh
Steven L
SL
Wijburg
Odilia L
OL
Strugnell
Richard A
RA
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology / Q000627:therapeutic use*; D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology / Q000627:therapeutic use*; D000942:Antigens, Bacterial / Q000276:immunology; D016667:Bacterial Capsules / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D007710:Klebsiella Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000276:immunology*; D008070:Lipopolysaccharides / Q000008:administration & dosage* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019081:O Antigens / Q000276:immunology; D012703:Serotyping; D014611:Vaccination
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D019081:O Antigens