title: Study of thrombopoietin for gene therapy of thrombocytopenia.
abstract: Thrombopoietin (TPO) is likely to he a potent, specific and reliable medication in the treatment of thrombocytopenia. A TPO-highly-expressed plasmid pcDNA3-TPO was constructed and a primary study was made on the expression of TPO cDNA in vitro and gene transfer study for thrombocytopenia in vivo. rhTPO showed complete and stable bioactivity by a series of indicators. High expression of TPO was detected in plasma from healthy mice or thrombocytopenia mice model receiving direct intramuscular injection of pcDNA3-TPO. And the platelet level of healthy mice peaked to 1.9-fold of baseline. Mice with CTX-induced thrombocytopenia achieved profound nadirs, acceleration of recovery, even 1.8-2.0-fold supranormal levels of peripheral platelet counts. The results offered experimental support for clinical application of gene therapy for thrombocytopenia via direct intramuscular injection of TPO cDNA.
authors: Institute of Genetics, Fudan University, Shanghai, China.
authors: S
authors: S
authors: Chong
authors: D
authors: D
authors: Lu
authors: C
authors: C
authors: Li
authors: X
authors: X
authors: Qiu
authors: J
authors: J
authors: Xue