[Gene expression profiles in human nasal polyps studied by DNA microarray].
OBJECTIVE
To explore the changes in gene expressions in the human nasal polyp.


METHODS
A 14500 gerie DNA microarray (Affymetrix) was used to examine gene expressions in 6 NP samples, 6 normal mucosal samples. The differentially expressed genes were identified and subjected to realtime PCR analysis.


RESULTS
The differentially expressed genes mostly involved in cytokines, adhesion genes about complements and their receptors, immune transcription regulatory molecules, signal transduction, differentially expressed genes including IL-8, RGS1, GRK4, CCL20, uteroglobin. The results of IL-8, RGS1 Real-time PCR of were consistent with that of gene chip analysis.


CONCLUSIONS
Microarray expression profile of NP samples is differential. RGS1 may play an important role in cell signal transduction, IL-8 may involve in occurrence of nasal polyps by inducing cells releasing inflammatory factors.
Liu
Bing
B
Department of Otorhinolaryngology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Wu
Jian
J
Fan
Jingping
J
Peng
Yucheng
Y
D000328:Adult; D000368:Aged; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult