[Protection against doxorubicin-induced oxidative damage in normal blood cells by naringenin].
The objective of this study was to investigate the protection by naringenin against doxorubicin-induced oxidative damage in normal blood cells. Inhibiting effects of naringenin, doxorubicin and naringenin combined with doxorubicind on K562 cells and polymorphonuclear leukocytes were detected with MTT method, the level of reactive oxygen species (ROS) and lipid peroxidation (MDA), the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were examined with spectrophotometric method in the K562 cells and polymorphonuclear leukocytes. The results indicated that the proliferation of K562 cells was not inhibited by the cytotoxicity of doxorubicin in combination of naringenin with doxorubicin. As compared with the doxorubicin, the addition of naringenin after doxorubicin for 1 hour, the levels of reactive oxygen species (ROS) and lipid peroxidation (MDA) obviously decreased, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) obviously increased in the polymorphonuclear leukocytes, but these were not changed obviously in K562 cells. It is concluded naringenin can protect against doxorubicin-induced oxidative damage in normal blood cells. The mechanism of naringenin may be elevating activities of antioxidant enzyme and degrading oxidative production level in normal blood cells, and meanswhile decreasing level of oxidative products.
Feng
Ying-Qian
YQ
Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China.
Zuo
Xue-Lan
XL
Li
Rui-Fang
RF
Zhang
Ke-Jian
KJ
Chen
Fei
F
Xiao
Hui
H
D000975:Antioxidants / Q000494:pharmacology*; D004317:Doxorubicin / Q000009:adverse effects*; D004912:Erythrocytes / Q000187:drug effects*; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D044950:Flavanones; D017382:Reactive Oxygen Species; D004317:Doxorubicin; D013482:Superoxide Dismutase; C005273:naringenin