title: Aspergillus fumigatus forms biofilms with reduced antifungal drug susceptibility on bronchial epithelial cells.
abstract: Aspergillus fumigatus is a leading cause of death in immunocompromised patients and a frequent colonizer of the respiratory tracts of asthma and cystic fibrosis (CF) patients. Biofilms enable bacteria and yeasts to persist in infections and can contribute to antimicrobial resistance. We investigated the ability of A. fumigatus to form biofilms on polystyrene (PS) and human bronchial epithelial (HBE) and CF bronchial epithelial (CFBE) cells. We developed a novel in vitro coculture model of A. fumigatus biofilm formation on HBE and CFBE cells. Biofilm formation was documented by dry weight, scanning electron microscopy (SEM), and confocal scanning laser microscopy (CSLM). The in vitro antifungal activities of seven antifungal drugs were tested by comparing planktonic and sessile A. fumigatus strains. A. fumigatus formed an extracellular matrix on PS and HBE and CFBE cells as evidenced by increased dry weight, SEM, and CSLM. These biofilms exhibited decreased antifungal drug susceptibility and were adherent to the epithelial cells, with fungi remaining viable throughout 3 days. These observations might have implications for treatment of A. fumigatus colonization in chronic lung diseases and for its potential impact on airway inflammation, damage, and infection.
authors: Department of Pediatrics III, Cystic Fibrosis Centre and Infectious Diseases, University of Heidelberg, Heidelberg, Germany.
authors: Marc J
authors: MJ
authors: Seidler
authors: Stefanie
authors: S
authors: Salvenmoser
authors: Frank-Michael C
authors: FM
authors: Müller
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000188:drug therapy / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D001980:Bronchi / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology*; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000188:drug therapy / Q000382:microbiology; D025141:Drug Resistance, Fungal; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D012141:Respiratory Tract Infections / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000935:Antifungal Agents