Bone marrow lacking integrin expression facilitates an enhanced susceptibility to EAE in the xenogeneic bone marrow chimeras.
Xenotransplantation of rat bone marrow cells (BMC) into immunodeficient (SCID) mice generates chimeric mice susceptible to paralytic autoimmune CNS inflammation. Herein, we identified a disease relevant subset of transplantable BMC lacking expression of CD11b/c and CD49d. Moreover, disease susceptibility was enhanced in the presence of non-myelin specific T-cells. Only the CD11b/c negative population of BM retained the capability to populate the blood, spleen and spinal cord of recipients and matured after transplant to express CD11b/c. These results indicate non-myelin T cells in combination with integrin negative BM represent pre-pathogenic determinants of an enhanced disease susceptibility to myelin reactive T cells.
Robinson
Kristine M
KM
V.A. Medical Center, R and D-23, 3710 SW Veterans Hospital Rd., Portland, OR 97239, United States.
Markwardt
Sheila
S
Kitzerow
Nick
N
Moes
Nicole
N
Estrada
Mary
M
Jones
Richard E
RE
D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000379:methods*; D039481:CD11b Antigen / Q000276:immunology / Q000378:metabolism; D039521:CD11c Antigen / Q000276:immunology / Q000378:metabolism; D017690:Cell Transplantation / Q000379:methods; D002678:Chimera; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000601:surgery*; D005260:Female; D016130:Immunophenotyping / Q000379:methods; D039423:Integrin alpha1 / Q000378:metabolism; D016023:Integrins / Q000172:deficiency*; D051379:Mice; D016513:Mice, SCID; D051381:Rats; D011917:Rats, Inbred Lew; D012720:Severity of Illness Index; D014183:Transplantation, Heterologous / Q000379:methods*
D039481:CD11b Antigen; D039521:CD11c Antigen; D039423:Integrin alpha1; D016023:Integrins