Cytokine concentrations in basal cell carcinomas of different histological types and localization.
BACKGROUND
Basal cell carcinoma (BCC) is the most common malignant skin tumor. Cytokines as major mediators of the immune system have been shown to play an important role in biology of the neoplasm with the general predomination of Th2 cytokines, whereas IFN-? and other Th1 cytokines are prevalent in spontaneously regressing tumors.


OBJECTIVE
We were interested in comparing cytokine levels in BCC and cutaneous squamous cell tumors with BCC of different localization and histological subtypes.


METHODS
Explants from freshly excised BCC from 18 patients, and cutaneous squamous cell tumors (solar keratoses and Bowen's disease) from 9 patients were cultivated for 24 h. Cytokine (IL- 2, IFN-gamma, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, TNFalpha, IL-1beta) concentrations in culture supernatants were determined by a sandwich immunoassay.


RESULTS
Tissue explants of BCC contained significantly higher concentrations of IL-1beta, IL-4, IL-5, and IL-6 compared to those of squamous cell tumors. Higher levels of TNF-alpha (p = 0.042), IL-4 (p = 0.028), and IL-5 (p = 0.012) were found in tumors localized to the head and neck compared to those on the trunk or extremities. Interleukin-6 concentrations were higher in aggressive BCC variants (infiltrative and micronodular), but the difference was not statistically significant (p = 0.068).


CONCLUSIONS
Confirming the earlier findings that BCC is a tumor with a Th2 cytokine microenvironment, this study further shows that BCC situated on the head and neck produce even more of certain Th2 cytokines (IL-4 and IL-5) and TNF-alpha, a crucial immunosuppressive cytokine released upon UVB irradiation.
Elamin
Issa
I
Department of Dermatovenereology, Military Medical Academy, Crnotravska 17, 11002 Belgrade, Serbia.
Zecević
Rados D
RD
Vojvodić
Danilo
D
Medenica
Ljiljana
L
Pavlović
Milos D
MD
D000368:Aged; D002280:Basal Cell Carcinoma / Q000737:chemistry*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D016207:Cytokines / Q000032:analysis*; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000032:analysis; D015848:Interleukin-5 / Q000032:analysis; D008297:Male; D012878:Skin Neoplasms / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D016207:Cytokines; D015848:Interleukin-5; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4