Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutininneuraminidase proteins.
Recombinant baculoviruses containing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein gene of the viscerotropic velogenic (vv) Newcastle disease virus (NDV) isolate, Kr-005/00, and a lentogenic La Sota strain of the NDV were constructed in an attempt to develop an effective subunit vaccine to the recent epizootic vvNDV. The level of protection was determined by evaluating the clinical signs, mortality, and virus shedding from the oropharynx and cloaca of chickens after a challenge with vvNDV Kr-005/00. The recombinant ND F (rND F) and recombinant HN (rND HN) glycoproteins derived from the velogenic strain provided good protection against the clinical signs and mortality, showing a 0.00 PI value and 100% protection after a booster immunization. On the other hand, the combined rND F + HN glycoprotein derived from the velogenic strain induced complete protection (0.00 PI value and 100% protection) and significantly reduced the amount of virus shedding even after a single immunization. The rND F and rND HN glycoproteins derived from the velogenic strain had a slightly, but not significantly, greater protective effect than the lentogenic strain. These results suggest that the combined rND F + HN glycoprotein derived from vvNDV can be an ideal subunit marker vaccine candidate in chickens in a future ND eradication program.
Lee
Youn-Jeong
YJ
National Veterinary Research and Quarantine Service, Anyang 430-824, Korea.
Sung
Haan-Woo
HW
Choi
Jun-Gu
JG
Lee
Eun-Kyoung
EK
Yoon
Hachung
H
Kim
Jae-Hong
JH
Song
Chang-Seon
CS
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000276:immunology*; D002645:Chickens / Q000821:virology*; D017931:DNA Primers; D005784:Gene Amplification; D015707:HN Protein / Q000235:genetics / Q000627:therapeutic use*; D007723:Korea; D008380:Marek Disease / Q000276:immunology / Q000517:prevention & control; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control*; D018411:Spodoptera / Q000821:virology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000627:therapeutic use; D014765:Viral Vaccines / Q000235:genetics / Q000627:therapeutic use
D017931:DNA Primers; D015707:HN Protein; D014614:Vaccines, Synthetic; D014765:Viral Vaccines