title: Biotransformation of acridine by Mycobacterium vanbaalenii.
abstract: Cultures of Mycobacterium vanbaalenii strain PYR-1 in a liquid medium were exposed to the toxic environmental contaminant acridine (260 microM). After incubation for 7 d, the cultures were extracted with ethyl acetate. Metabolites were purified using high-performance liquid chromatography and analyzed by mass spectrometry and 1H nuclear magnetic resonance spectroscopy. Four metabolites, 9,10-dihydroacridine, 4-hydroxyacridine, acridine cis-1 ,2-dihydrodiol, and acridin-9(10H)-one, were identified.
authors: Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, 3900 NCTR Road, Jefferson, Arkansas 72079, USA. john.sutherland@fda.hhs.gov
authors: John B
authors: JB
authors: Sutherland
authors: Thomas M
authors: TM
authors: Heinze
authors: Mason G
authors: MG
authors: Pearce
authors: Joanna
authors: J
authors: Deck
authors: Anna J
authors: AJ
authors: Williams
authors: James P
authors: JP
authors: Freeman
mesh_terms: D000166:Acridines / Q000493:pharmacokinetics*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D009161:Mycobacterium / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000166:Acridines