[Redox-dependent regulation of apoptosis as a function of intracellular reactive oxygen species in oxidative stress].
Programmed cell death of mononuclear cells in conditions of oxidative stress in vitro and selective inhibitors of MAP-kinases JNK, p38 were investigated. Levels of active and inactive forms of MAP-kinases, factors of transcription P53, NF-kB and proteins-regulators of apoptosis Bcl-X(L), Bad, Bcl-2 were determined by immunoblotting (Western blotting). The increasing of number of annexin-plus mononuclears/lymphocytes in the culture associated with enhance of the level of intracellular reactive oxygen species was shown. The treatment of selective inhibitors JNK SP600125 and p38 ML3403 in vitro prevents peroxide-induced appearance of P53 and NF-kB in blood mononuclear cells, associated with increasing of their apoptotic activity. The disturbance of the balance of pro- and antiapoptotic proteins of Bcl-2, family (the increase of the Bax level without changes of Bcl-X(L) and Bcl-2) leads to the growth of apoptosis process of mononuclear leucocytes activity in oxidative stress conditions.
Riazantseva
N V
NV
Novitskiĭ
V V
VV
Chasovskikh
N Iu
NIu
Kaĭgorodova
E V
EV
Starikova
E G
EG
Starikov
Iu V
IuV
D000293:Adolescent; D000328:Adult; D000873:Anthracenes / Q000494:pharmacology; D017209:Apoptosis; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D048670:MAP Kinase Kinase 4 / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000096:biosynthesis; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
C522498:(4-(5-(4-fluorophenyl)-2-methylsulfanyl-3H-imidazol-4-yl)pyridin-2-yl)-(1-phenylethyl)amine; D000873:Anthracenes; D007093:Imidazoles; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D011725:Pyridines; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; C432165:pyrazolanthrone; D048051:p38 Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4