C. elegans and H. sapiens mRNAs with edited 3' UTRs are present on polysomes.
Adenosine deaminases that act on RNA (ADARs) are editing enzymes that convert adenosine to inosine in double-stranded RNA (dsRNA). ADARs sometimes target codons so that a single mRNA yields multiple protein isoforms. However, ADARs most often target noncoding regions of mRNAs, such as untranslated regions (UTRs). To understand the function of extensive double-stranded 3' UTR structures, and the inosines within them, we monitored the fate of reporter and endogenous mRNAs that include structured 3' UTRs in wild-type Caenorhabditis elegans and in strains with mutations in the ADAR genes. In general, we saw little effect of editing on stability or translatability of mRNA, although in one case an ADR-1 dependent effect was observed. Importantly, whereas previous studies indicate that inosine-containing RNAs are retained in the nucleus, we show that both C. elegans and Homo sapiens mRNAs with edited, structured 3' UTRs are present on translating ribosomes.
Hundley
Heather A
HA
Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112, USA.
Krauchuk
Ammie A
AA
Bass
Brenda L
BL
D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D006801:Humans; D009154:Mutation; D009690:Nucleic Acid Conformation; D011132:Polyribosomes / Q000378:metabolism*; D017393:RNA Editing; D018510:RNA, Helminth / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins
D020413:3' Untranslated Regions; D029742:Caenorhabditis elegans Proteins; D018510:RNA, Helminth; D016601:RNA-Binding Proteins; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase