Herpes viruses in periodontal compromised sites: comparison between HIV-positive and -negative patients.
OBJECTIVE
The objective of this study was to compare the frequency of herpes simplex virus type 1 (HSV-1), Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) in subgingival plaque, saliva and peripheral blood of HIV-positive and-negative patients with periodontal disease.


METHODS
Fifty HIV-positive subjects (23 with gingivitis, 27 with periodontitis) and 50 healthy HIV-negative patients with chronic periodontitis were included in the study. Parameters of probing depth (PD), clinical attachment level (CAL), gingival index and plaque index were recorded. The samples were processed for viral identification by the nested polymerase chain reaction technique.


RESULTS
HCMV was the most prevalent virus in HIV-positive (82%) and-negative patients (84%), and the detection in the three samples was similar (p>0.05). HSV-1 was the least prevalent virus in both groups, being detected in similar frequencies in oral sites and in peripheral blood. EBV-1 was found more frequently in saliva and subgingival plaque of HIV-positive patients than in HIV-negative patients (p< or =0.05).


CONCLUSIONS
EBV-1 was more frequently recovered in oral sites of HIV-positive patients than in HIV-negative patients.
Grande
Sabrina Rosa
SR
Department of Periodontology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Imbronito
Ana Vitória
AV
Okuda
Osmar Shizuo
OS
Lotufo
Roberto Fraga Moreira
RF
Magalhães
Marina Helena Gallottini
MH
Nunes
Fabio Daumas
FD
D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000097:blood / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D003773:Dental Plaque / Q000821:virology; D005260:Female; D005891:Gingivitis / Q000097:blood / Q000150:complications / Q000821:virology*; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000097:blood / Q000150:complications* / Q000821:virology; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D010518:Periodontitis / Q000097:blood / Q000150:complications / Q000821:virology*; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012463:Saliva / Q000821:virology; D018709:Statistics, Nonparametric
D004279:DNA, Viral