An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads.
This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA or DNA.
Lim
Crystale Siew Ying
CS
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.
Tung
Chee Hong
CH
Rosli
Rozita
R
Chong
Pei Pei
PP
D002175:Candida / Q000737:chemistry* / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D005821:Genetic Techniques; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D013012:Sorbitol / Q000737:chemistry
D004271:DNA, Fungal; D012331:RNA, Fungal; D013012:Sorbitol