title: TiO2 nanowire bundle microelectrode based impedance immunosensor for rapid and sensitive detection of Listeria monocytogenes.
abstract: A novel TiO 2 nanowire bundle microelectrode based immunosensor was demonstrated as a more sensitive, specific, and rapid technology for detection of Listeria monocytogenes. TiO 2 nanowire bundle was prepared through a hydrothermal reaction of alkali with TiO 2 powder and connected to gold microelectrodes with mask welding. Monoclonal antibodies were immobilized on the surface of a TiO 2 nanowire bundle to specifically capture L. monocytogenes. Impedance change caused by the nanowire-antibody-bacteria complex was measured and correlated to bacterial number. This nanowire bundle based immunosensor could detect as low as 10 (2) cfu/ml of L. monocytogenes in 1 h without significant interference from other foodborne pathogens.
authors: Department of Biological and Agricultural Engineering, Cell and Molecular Biology Program, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, Arkansas 72701, USA.
authors: Ronghui
authors: R
authors: Wang
authors: Wenjun
authors: W
authors: Dong
authors: Chuanmin
authors: C
authors: Ruan
authors: Damira
authors: D
authors: Kanayeva
authors: Ryan
authors: R
authors: Tian
authors: Kentu
authors: K
authors: Lassiter
authors: Yanbin
authors: Y
authors: Li
mesh_terms: D015374:Biosensing Techniques; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D053770:Nanowires; D014025:Titanium / Q000737:chemistry*
chemical_list: C009495:titanium dioxide; D014025:Titanium