[The relationship between the degradation of actin and the postmortem interval in rats].
OBJECTIVE
To observe the degradation of actin in cardiac muscle, brain and skeletal muscle of rats after death and to find an objective parameter interval (PMI) estimation.


METHODS
Twenty eight clear Sprague Dawley rats put into an artificial climate incubator (set at 20 degrees C) for 0, 24, 48, 72, The actin contents in the above tissues were quantitated by Western-blot Pro Plus 5.0 image analysis system, and were then statistically analyzed


RESULTS
Actin content in all these tissues decreased gradually with prolonged differences between the groups was statistically significant (P < 0.05), with fastest, then the lung, the spleen, the liver, the kidney, the cardiac muscle order. There was a strong correlation between actin degradation and determination (R2) exceeded 0.75 in all these tissues.


CONCLUSIONS
degradation, the actin contents in cardiac muscle, liver, spleen, lung, kindey, rats decreased gradually with prolonged PMI, which may potentially be PMI estimation.
Liu
Yang
Y
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
Kuai
Jin-Xia
JX
Zhang
Yan-Wei
YW
Wang
Ying-Yuan
YY
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003643:Death; D005260:Female; D049429:Forensic Pathology / Q000379:methods*; D008099:Liver / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D011180:Postmortem Changes; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D000199:Actins