[Cultivable microbial diversity at the rhizosphere of Phyllostachys pubescens].
OBJECTIVE
To obtain the information of the cultivable microbial population diversity at the rhizosphere of Phyllostachys pubescens.


METHODS
We isolated strains from Tianmu Mountain and Jinyun Mountain by diluting plate counting method and analyzed the 16S rDNA sequence of the isolates.


RESULTS
We obtained 51 and 31 strains with different morphological character of colonies from Tianmu Mountain and Jinyun Mountain separately. The 16S rDNA sequence analysis showed that they had similar microbial population diversity. There were 40% and 58% firmicutes, 36.7% and 10.52% actinobacteria, 10% and 5.26% alphaproteobacteria, 10% and 26.32% gammaproteobacteria at the rhizosphere of Phyllostachys pubescens from Tianmu Mountain and Jinyun Mountain separately. The dominant bacteria were the genera Bacillus in both two areas.


CONCLUSIONS
The result showed that the cultivable microbial population diversity was abundant and there were some potential novel strains at the rhizosphere of Phyllostachys pubescens. Our research made it is possible to further investigate the function of the rhizosphere microbes and there interaction with the bamboo plant.
Li
Lubin
L
Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China. lilubin@126.com
Liu
Min
M
Yang
Shuzhen
S
Liu
Liang
L
Miao
Kun
K
Yang
Kai
K
Han
Jigang
J
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D010802:Phylogeny; D018517:Plant Roots; D006109:Poaceae / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal