title: Possible relation between histone 3 and cytokeratin 18 in human hepatocellular carcinoma.
abstract: BACKGROUND
  Previously we found some low molecular weight proteins identified as histone in hepatocelluar carcinoma. Our objective was to clarify whether the coimmunoprecipitation of histone and cytokeratin 18 was an artifact or not.
  
  
  METHODS
  Histone 3 and cytokeratin 18 were investigated in three cases of human hepatocellular carcinoma and one case of normal liver tissue. Nuclei of the tissues were isolated; the proteins inside the nuclei were analyzed by Western blot.
  
  
  RESULTS
  The results revealed histone was co-immunoprecipitated with cytokeratin 18 in hepatocellular carcinoma. It was speculated that modulation of the cytoskeleton in human hepatocellular carcinoma might disturb the organization of the nucleoskeleton. The unstable nucleoskeleton might further cause instability and fragility of nuclei, thus possibly exposing the histone and co-immunoprecipitating it with cytokeratin 18.
  
  
  CONCLUSIONS
  The evidence might indicate that expression of histone 3 was highly related to modulation of cytokeratin 18 and might play an important role in tumorigenesis of hepatocellular carcinoma.
authors: Department of Nursing, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C.
authors: Chin-Chin
authors: CC
authors: Ho
authors: Chiung-Chi
authors: CC
authors: Cheng
authors: Yi-Hsiang
authors: YH
authors: Liu
authors: Ren-Jeng
authors: RJ
authors: Pei
authors: Yung-Hsiang
authors: YH
authors: Hsu
authors: Kun-Tu
authors: KT
authors: Yeh
authors: Lu-Chang
authors: LC
authors: Ho
authors: Ming-Chuang
authors: MC
authors: Tsai
authors: Yih-Shyong
authors: YS
authors: Lai
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006657:Histones / Q000096:biosynthesis / Q000502:physiology*; D006801:Humans; D047468:Immunoprecipitation; D053538:Keratin-18 / Q000096:biosynthesis / Q000502:physiology*; D007633:Keratins / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism*
chemical_list: D002843:Chromatin; D006657:Histones; D053538:Keratin-18; D007633:Keratins