The block of intracellular calcium release affects the pollen tube development of Picea wilsonii by changing the deposition of cell wall components.
Two potent drugs, neomycin and TMB-8, which can block intracellular calcium release, were used to investigate their influence on pollen tube growth and cell wall deposition in Picea wilsonii. Apart from inhibiting pollen germination and pollen tube growth, the two drugs largely influenced tube morphology. The drugs not only obviously disturbed the generation and maintenance of the tip-localized Ca(2+) gradient but also led to a heavy accumulation of callose at the tip region of P. wilsonii pollen tubes. Fourier transform infrared (FTIR) spectroscopy analysis showed that the deposition of cell wall components, such as carboxylic acid, pectins, and other polysaccharides, in pollen tubes was changed by the two drugs. The results obtained from immunolabeling with different pectin and arabinogalactan protein antibodies agreed well with the FTIR results and further demonstrated that the generation and maintenance of the gradient of cross-linked pectins, as well as the proportional distribution of arabinogalactan proteins in tube cell walls, are essential for pollen tube growth. These results strongly suggest that intracellular calcium release mediates the processes of pollen germination and pollen tube growth in P. wilsonii and its inhibition can lead to abnormal growth by disturbing the deposition of cell wall components in pollen tube tips.
Chen
Kun-Ming
KM
Institute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou.
Wu
Guo-Li
GL
Wang
Yu-Hua
YH
Tian
Cui-Ting
CT
Samaj
Jozef
J
Baluska
Frantisek
F
Lin
Jin-Xing
JX
D002118:Calcium / Q000378:metabolism*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D018525:Germination / Q000187:drug effects; D005936:Glucans / Q000378:metabolism; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009088:Mucoproteins / Q000378:metabolism; D009355:Neomycin / Q000494:pharmacology; D010368:Pectins / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D028222:Picea / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D010940:Plant Proteins / Q000378:metabolism; D053205:Pollen Tube / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D015398:Signal Transduction / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared
D005936:Glucans; D009088:Mucoproteins; D010716:Phosphatidylinositols; D010940:Plant Proteins; C006619:arabinogalactan proteins; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D010368:Pectins; C048306:callose; D009355:Neomycin; D002118:Calcium