title: Ligand-based virtual screening to identify new T-type calcium channel blockers.
abstract: T-type calcium channels are involved in the generation of rhythmical firing patterns in the mammalian central nervous system and in various pathological alterations of neuronal excitability such as in epilepsy or neuropathic pain. In the search for new T-type calcium channel blockers that would help to treat these disorders, we have followed a bi-dimensional pharmacophore-based virtual screening approach to identify new inhibitors. Nineteen molecules extracted from AurSCOPE Ion Channels knowledgebase were used as query molecules to screen an external database. This in silico approach was then validated using electrophysiology. Interestingly, 16 compounds out of 38 distinct molecules tested showed more than 50% blockade of the Ca(V)3.2 mediated T-type current. Two series of compounds show chemical originality compared with known T-type calcium channel blockers.
authors: Aureus Pharma, Paris, France.
authors: Ismail
authors: I
authors: Ijjaali
authors: Christian
authors: C
authors: Barrere
authors: Joel
authors: J
authors: Nargeot
authors: François
authors: F
authors: Petitet
authors: Emmanuel
authors: E
authors: Bourinet
mesh_terms: D000818:Animals; D020747:Calcium Channels, T-Type / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D017076:Computer-Aided Design; D016208:Databases, Factual; D015195:Drug Design; D006801:Humans; D008024:Ligands; D008564:Membrane Potentials; D015394:Molecular Structure; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D015203:Reproducibility of Results; D013329:Structure-Activity Relationship; D014162:Transfection; D014584:User-Computer Interface
chemical_list: C479952:CACNA1H protein, human; D020747:Calcium Channels, T-Type; D008024:Ligands