title: The effect of devitalized trabecular bone on the formation of osteochondral tissue-engineered constructs.
abstract: In the current study, evidence is presented demonstrating that devitalized trabecular bone has an inhibitory effect on in vitro chondral tissue development when used as a base material for the tissue-engineering of osteochondral constructs for cartilage repair. Chondrocyte-seeded agarose hydrogel constructs were cultured alone or attached to an underlying bony base in a chemically defined medium formulation that has been shown to yield engineered cartilaginous tissue with native Young's modulus (E(Y)) and glycosaminoglycan (GAG) content. By day 42 in culture the incorporation of a bony base significantly reduced these properties (E(Y)=87+/-12 kPa, GAG=1.9+/-0.8%ww) compared to the gel-alone group (E(Y)=642+/-97 kPa, GAG=4.6+/-1.4%ww). Similarly, the mechanical and biochemical properties of chondrocyte-seeded agarose constructs were inhibited when co-cultured adjacent to bone (unattached), suggesting that soluble factors rather than direct cell-bone interactions mediate the chondro-inhibitory bone effects. Altering the method of bone preparation, including demineralization, or the timing of bone introduction in co-culture did not ameliorate the effects. In contrast, osteochondral constructs with native cartilage properties (E(Y)=730+/-65 kPa, GAG=5.2+/-0.9%ww) were achieved when a porous tantalum metal base material was adopted instead of bone. This work suggests that devitalized bone may not be a suitable substrate for long-term cultivation of osteochondral grafts.
authors: Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace MC8904, 1210 Amsterdam Avenue, New York, NY 10027, United States.
authors: Eric G
authors: EG
authors: Lima
authors: Pen-Hsiu
authors: PH
authors: Grace Chao
authors: Gerard A
authors: GA
authors: Ateshian
authors: B Sonny
authors: BS
authors: Bal
authors: James L
authors: JL
authors: Cook
authors: Gordana
authors: G
authors: Vunjak-Novakovic
authors: Clark T
authors: CT
authors: Hung
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D018920:Coculture Techniques; D003094:Collagen / Q000378:metabolism; D016062:Porosity; D013635:Tantalum / Q000737:chemistry; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D013635:Tantalum; D003094:Collagen