title: Identification of cis-acting sites for condensin loading onto budding yeast chromosomes.
abstract: Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes. Here, we analyze condensin binding to budding yeast chromosomes using high-resolution oligonucleotide tiling arrays. Condensin-binding sites coincide with those of the loading factor Scc2/4 of the related cohesin complex. The sites map to tRNA and other genes bound by the RNA polymerase III transcription factor TFIIIC, and ribosomal protein and SNR genes. An ectopic B-box element, recognized by TFIIIC, constitutes a minimal condensin-binding site, and TFIIIC and the Scc2/4 complex promote functional condensin association with chromosomes. A similar pattern of condensin binding is conserved along fission yeast chromosomes. This reveals that TFIIIC-binding sites, including tRNA genes, constitute a hitherto unknown chromosomal feature with important implications for chromosome architecture during both interphase and mitosis.
authors: Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK.
authors: Claudio
authors: C
authors: D'Ambrosio
authors: Christine Katrin
authors: CK
authors: Schmidt
authors: Yuki
authors: Y
authors: Katou
authors: Gavin
authors: G
authors: Kelly
authors: Takehiko
authors: T
authors: Itoh
authors: Katsuhiko
authors: K
authors: Shirahige
authors: Frank
authors: F
authors: Uhlmann
mesh_terms: D000251:Adenosine Triphosphatases / Q000502:physiology*; D001665:Binding Sites; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D042002:Chromatin Assembly and Disassembly; D047369:Chromatin Immunoprecipitation; D002868:Chromosomal Proteins, Non-Histone; D015825:Chromosomes, Fungal / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000502:physiology*; D005656:Fungal Proteins / Q000502:physiology; D008938:Mitosis; D046912:Multiprotein Complexes / Q000502:physiology*; D012320:RNA Polymerase III / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics; D004718:Saccharomycetales / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D019474:Transcription Factors, TFIII / Q000502:physiology*; D014158:Transcription, Genetic; D000097722:Cohesins; D000098832:Transcription Factor TFIIIC
chemical_list: D000251:Adenosine Triphosphatases; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D046912:Multiprotein Complexes; D012320:RNA Polymerase III; D012343:RNA, Transfer; D019474:Transcription Factors, TFIII; D000097722:Cohesins; D000098832:Transcription Factor TFIIIC; C108312:condensin complexes