Bacterial endotoxin (LPS)-induced DNA damage in preimplanting embryonic and uterine cells inhibits implantation.
OBJECTIVE
To investigate lipopolysaccharide (LPS)-induced DNA damage in preimplanting embryonic and uterine cells during preimplantation period of pregnancy that may ultimately inhibit the process of implantation in mouse.


METHODS
Animal study.


METHODS
Academic research environment.


METHODS
Sixty four Park strain female mice.


METHODS
The "minimum dose" (MD) of LPS was injected intraperitoneally in the pregnant females on day 0.5 of pregnancy, and individual embryos and uterine cells were assessed by comet assay on days 1.5, 2.5, 3.5, and 4.375 of the preimplantation period of pregnancy.


METHODS
Percentage of embryos and uterine cells with tail, mean comet tail length, percentage of fragmented DNA in tail.


RESULTS
Significantly higher numbers of embryos with higher mean comet tail length and percentage of fragmented DNA in tail were observed in the LPS-treated compared with control animals as the period of pregnancy approaches the stage of implantation. At the same time, DNA damage was also significantly higher in the uterine cells of LPS-treated compared with control animals.


CONCLUSIONS
The MD of LPS can induce DNA damage in the preimplantation-stage embryos and uterine cells, which causes poor embryonic development and improper preparation of uterine horns during the preimplantation period of pregnancy, which may ultimately inhibit the process of implantation in mouse.
Jaiswal
Yogesh Kumar
YK
Molecular Biology and Reproductive Immunology Laboratory, School of Studies in Biochemistry, Jiwaji University, Gwalior, India. jaiswalyk@gmail.com
Jaiswal
Mukesh Kumar
MK
Agrawal
Varkha
V
Chaturvedi
Madan Mohan
MM
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000473:pathology*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D010064:Embryo Implantation / Q000187:drug effects*; D004622:Embryo, Mammalian / Q000187:drug effects / Q000473:pathology; D004971:Estrus / Q000187:drug effects; D005260:Female; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D014599:Uterus / Q000187:drug effects / Q000473:pathology / Q000502:physiology*
D008070:Lipopolysaccharides