A nitty-gritty aspect of correlation and network inference from gene expression data.
BACKGROUND
All currently available methods of network/association inference from microarray gene expression measurements implicitly assume that such measurements represent the actual expression levels of different genes within each cell included in the biological sample under study. Contrary to this common belief, modern microarray technology produces signals aggregated over a random number of individual cells, a "nitty-gritty" aspect of such arrays, thereby causing a random effect that distorts the correlation structure of intra-cellular gene expression levels.


RESULTS
This paper provides a theoretical consideration of the random effect of signal aggregation and its implications for correlation analysis and network inference. An attempt is made to quantitatively assess the magnitude of this effect from real data. Some preliminary ideas are offered to mitigate the consequences of random signal aggregation in the analysis of gene expression data.


CONCLUSIONS
Resulting from the summation of expression intensities over a random number of individual cells, the observed signals may not adequately reflect the true dependence structure of intra-cellular gene expression levels needed as a source of information for network reconstruction. Whether the reported effect is extrime or not, the important point, is to reconize and incorporate such signal source for proper inference. The usefulness of inference on genetic regulatory structures from microarray data depends critically on the ability of investigators to overcome this obstacle in a scientifically sound way.


METHODS
This article was reviewed by Byung Soo KIM, Jeanne Kowalski and Geoff McLachlan.
Klebanov
Lev B
LB
Department of Probability and Statistics, Charles University, Sokolovska 83, Praha-8, CZ-18675, Czech Republic. levkleb@yahoo.com
Yakovlev
Andrei Yu
AY
D000818:Animals; D019295:Computational Biology / Q000379:methods / Q000706:statistics & numerical data; D020869:Gene Expression Profiling / Q000379:methods / Q000706:statistics & numerical data*; D006801:Humans; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000706:statistics & numerical data*; D018709:Statistics, Nonparametric