title: Membrane glycoproteins associated with breast tumor cell progression identified by a lectin affinity approach.
abstract: The membrane glycoprotein component of the cellular proteome represents a promising source for potential disease biomarkers and therapeutic targets. Here we describe the development of a method that facilitates the analysis of membrane glycoproteins and apply it to the differential analysis of breast tumor cells with distinct malignant phenotypes. The approach combines two membrane extraction procedures, and enrichment using ConA and WGA lectin affinity columns, prior to digestion and analysis by LC-MS/MS. The glycoproteins are identified and quantified by spectral counting. Although the distribution of glycoprotein expression as a function of MW and p I was very similar between the two related cell lines tested, the approach enabled the identification of several distinct membrane glycoproteins with an expression index correlated with either a precancerous (MCF10AT1), or a malignant, metastatic cellular phenotype (MCF10CA1a). Among the proteins associated with the malignant phenotype, Gamma-glutamyl hydrolase, CD44, Galectin-3-binding protein, and Syndecan-1 protein have been reported as potential biomarkers of breast cancer.
authors: Department of Chemistry, The University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109-1055, USA.
authors: Yanfei
authors: Y
authors: Wang
authors: Xiaoping
authors: X
authors: Ao
authors: Huy
authors: H
authors: Vuong
authors: Meghana
authors: M
authors: Konanur
authors: Fred R
authors: FR
authors: Miller
authors: Steve
authors: S
authors: Goodison
authors: David M
authors: DM
authors: Lubman
mesh_terms: D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity / Q000379:methods*; D005260:Female; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D013058:Mass Spectrometry; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D037102:Lectins; D008562:Membrane Glycoproteins; D010455:Peptides