title: Proteomic identification of the silkworm (Bombyx mori L) prothoracic glands during the fifth instar stage.
abstract: Although the ecdysteroid of the silkworm had been studied for decades, the proteome of the prothoracic gland, the primary source of ecdysteroid hormones, has not been studied previously. In the present paper, we utilized a proteomic approach to investigate the fifth instar prothoracic gland during the growth and development of the silkworm, Bombyx mori L. The two-dimensional electrophoresis results showed that the majority of proteins were acidic proteins, especially concentrated in the area of 25-65 kDa, with pI values of between 4 and 7, and the difference was not distinct. When compared with Qiufeng (Japanese strain), the interspecific distinction was larger than the intraspecific distinction, and 19 particular spots, excized from the third, fifth and ninth days of p50 (Chinese strain) and Qiufeng were subjected to MALDI-TOF-MS (matrix-assisted laser-desorption ionization-time-of-flight MS) analysis. We sorted them into seven catagories: energetics and/or metabolism, storage proteins, protection, lipid metabolism, signal transduction, cell function and unknown function proteins. Of these proteins, arginine methyltransferase is discussed as playing an important role in regulating the activation of ecdysteroidogenesis via transcription or translation.
authors: Key Laboratory of Animal Epidemic Etiology and Immunological Prevention of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310029, People's Republic of China.
authors: Xiao-feng
authors: XF
authors: Wu
authors: Xing-hua
authors: XH
authors: Li
authors: Wan-fu
authors: WF
authors: Yue
authors: Bhaskar
authors: B
authors: Roy
authors: Guang-li
authors: GL
authors: Li
authors: Jian-mei
authors: JM
authors: Liu
authors: Bo-xiong
authors: BX
authors: Zhong
authors: Qi-kang
authors: QK
authors: Gao
authors: Wan Chi Cheong
authors: WC
authors: David
authors: Yun-gen
authors: YG
authors: Miao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000254:growth & development* / Q000378:metabolism; D003335:Corpora Allata / Q000254:growth & development* / Q000378:metabolism; D026461:Ecdysteroids / Q000235:genetics / Q000378:metabolism; D019476:Insect Proteins / Q000032:analysis*; D007814:Larva / Q000254:growth & development; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D047011:Silk / Q000096:biosynthesis
chemical_list: D026461:Ecdysteroids; D019476:Insect Proteins; D020543:Proteome; D047011:Silk