Analysis of the association of preeclampsia with polymorphisms of the INS, INSR and IRS1 genes in Mexican women.
OBJECTIVE
It has been proposed that preeclampsia is a metabolic syndrome of pregnancy. The polymorphisms PstI and MaeIII of INS, NsiI of INSR and Ala513Pro and Gly972Arg of IRS1 have been associated with metabolic syndrome; moreover, the products of these genes are functionally contiguous during insulin signaling. The aim of this study was to assess whether these polymorphisms are associated with preeclampsia.


METHODS
46 normotensive pregnant women and 43 preeclamptic patients were included in the study to develop a clinical, biochemical and genotypic profile of preeclampsia. Clinical evaluation consisted of measurement of blood pressure, height and weight. Peripheral blood samples were collected for determination of fasting glucose and insulin concentrations and for extraction of genomic DNA. Proteinuria was determined. Polymorphisms were detected using PCR-RFLP.


RESULTS
The normotensive and preeclampsia groups did not differ significantly in clinical and biochemical traits, except for systolic and diastolic blood pressure (p < 0.0001). Polymorphisms previously associated with metabolic syndrome in Mexican populations were not associated with preeclampsia in Mexican women (p > 0.05).


CONCLUSIONS
The lack of an association between preeclampsia and the polymorphisms studied suggests that other genes whose products do not have direct functional interaction with metabolic syndrome or epigenetic factors may play a role in preeclampsia.
Machorro-Lazo
Maria-Victoria
MV
División de Medicina Molecular, CIBO, IMSS, Guadalajara, Mexico.
Sanchez-Corona
Jose
J
Martínez-Abundis
Esperanza
E
González-Ortiz
Manuel
M
Galaviz-Hernandez
Carlos
C
Perea
Francisco-Javier
FJ
Garcia-Zapien
Alejandra-Guadalupe
AG
Cruz-Quevedo
Edhit-Guadalupe
EG
Salgado-Goytia
Lorenzo
L
Moran-Moguel
Maria-Cristina
MC
Flores-Martínez
Silvia-Esperanza
SE
D000328:Adult; D000483:Alleles; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D003430:Cross-Sectional Studies; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005260:Female; D006239:Haplotypes; D006801:Humans; D007328:Insulin / Q000097:blood / Q000235:genetics*; D055504:Insulin Receptor Substrate Proteins / Q000097:blood / Q000235:genetics*; D008800:Mexico; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000097:blood / Q000235:genetics*; D011247:Pregnancy; D011972:Receptor, Insulin / Q000097:blood / Q000235:genetics*; D055815:Young Adult
D001786:Blood Glucose; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; D004247:DNA; D011972:Receptor, Insulin