A rapid and easy multiresidue method for the determination of pesticide residues in vegetables, fruits, and cereals using liquid Chromatography/tandem mass spectrometry.
The applicability of a rapid and easy multiresidue method for determination of pesticide residues in agricultural products by using liquid chromatography/tandem mass spectrometry (LC/MS/MS) was examined. Pesticide residues were extracted with acetonitrile in a disposable tube using a homogenizer, followed by salting out with anhydrous magnesium sulfate and sodium chloride. The extract was purified with a double-layered cartridge column (graphite carbon black/primary-secondary amine silica gel). After removal of the solvent, the extract was resolved in methanol-water and analyzed with LC/MS/MS. Recovery tests of 99 pesticide residues from 7 agricultural products were performed at 20 and 100 ng/g. Throughout all of the agricultural products tested, 47 pesticides exhibited satisfactory recoveries (70-120%) and relative standard deviations (<20%) at both concentrations. The time for processing of 12 samples to test solutions was approximately 2-3 h. This method could be useful for determination of pesticide residues in agricultural products.
Takatori
Satoshi
S
Osaka Prefectural Institute of Public Health, Division of Food Chemistry, Nakamichi 1-3-69, Higashinari-ku, Osaka 537-0025, Japan. sts-tkt@iph.pref.osaka.jp
Okihashi
Masahiro
M
Okamoto
You
Y
Kitagawa
Yoko
Y
Kakimoto
Sachiko
S
Murata
Hiroshi
H
Sumimoto
Tatsuo
T
Tanaka
Yukio
Y
D002853:Chromatography, Liquid; D003198:Computer Simulation; D002523:Edible Grain / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D007202:Indicators and Reagents; D007881:Legislation, Food; D010573:Pesticide Residues / Q000032:analysis*; D010647:Phenyl Ethers; D011422:Propionates / Q000737:chemistry; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014675:Vegetables / Q000737:chemistry*
C447201:2-phenoxypropionic acid; D007202:Indicators and Reagents; D010573:Pesticide Residues; D010647:Phenyl Ethers; D011422:Propionates