Monitoring of hormonal drug effect in a single breast cancer cell using an estrogen responsive GFP reporter vector delivered by a nanoneedle.
In this study, we have evaluated a sensor system for a hormonal drug effect in a single cell level using a novel low invasive single cell DNA delivery technology using a nanoneedle. An estrogen responsive GFP reporter vector (pEREGFP9) was constructed and its estrogenic response activity was confirmed in breast cancer cells (MCF-7) using lipofection as the means of transferring the vector to the cells. The pEREGFP9 vector was delivered to a single MCF-7 using a nanoneedle and the effect of ICI 182,780, which is an antagonist of estrogen, was observed using the GFP expression level. By ICI 182,780 treatment, the fluorescence intensity of the GFP was decreased by 30-50% within 24h. This technology is the very first trial of single cell diagnosis and we are looking forward to applying it to precious single cell diagnosis in medical fields.
Han
Sung-Woong
SW
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
Nakamura
Chikashi
C
Imai
Yosuke
Y
Nakamura
Noriyuki
N
Miyake
Jun
J
D018931:Antineoplastic Agents, Hormonal / Q000008:administration & dosage; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D001943:Breast Neoplasms / Q000473:pathology / Q000503:physiopathology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000008:administration & dosage; D004958:Estradiol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D000077267:Fulvestrant; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D008845:Microinjections / Q000295:instrumentation* / Q000379:methods; D009339:Needles; D014162:Transfection / Q000295:instrumentation* / Q000379:methods
D018931:Antineoplastic Agents, Hormonal; D049452:Green Fluorescent Proteins; D000077267:Fulvestrant; D004958:Estradiol; D004247:DNA