title: Identification of DNA-reactive B cells in patients with systemic lupus erythematosus.
abstract: Autoreactive B cells play a central role in systemic lupus erythematosus (SLE). Characterization of DNA-reactive B cells in the blood of lupus patients has been limited by the low frequency of the population. Using a tetrameric configuration of a peptide mimetope of DNA, we identified peptide-reactive B cells in peripheral blood. Antibodies derived from these B cells bound to peptide and were largely cross-reactive to dsDNA. This methodology enables us to track the development of autoreactive B cells, which recognize peptide and dsDNA, in individual patients with SLE and permits the isolation of autoreactive B cells for further characterization.
authors: Feinstein Institute for Medical Research, North Shore-Long Island Jewish Health System, Manhasset, New York 11030, USA.
authors: Jie
authors: J
authors: Zhang
authors: Annett M
authors: AM
authors: Jacobi
authors: Meggan
authors: M
authors: Mackay
authors: Cynthia
authors: C
authors: Aranow
authors: Tao
authors: T
authors: Wang
authors: Prameladevi
authors: P
authors: Chinnasamy
authors: Betty
authors: B
authors: Diamond
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*
chemical_list: D004247:DNA