The phage K lytic enzyme LysK and lysostaphin act synergistically to kill MRSA.
LysK is the endolysin from the staphylococcal bacteriophage K, and can digest the cell wall of many staphylococci. Lysostaphin is a bacteriocin secreted by Staphylococcus simulans to kill Staphylococcus aureus. Both LysK and lysostaphin have been shown to lyse methicillin-resistant S. aureus (MRSA). This study describes optimal reaction conditions for the recombinant His-tagged LysK protein (pH range pH 6-10, and 0.3-0.5 M NaCl), and C-His-LysK MIC (32.85+/-4.87 mug mL(-1)). LysK and lysostaphin demonstrate antimicrobial synergy by the checkerboard assay.
Becker
Stephen C
SC
Animal Biosciences and Biotechnology Laboratory, Animal and Natural Resources Institute, Agricultural Research Service-U.S. Department of Agriculture, Beltsville Agricultural Research Center, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.
Foster-Frey
Juli
J
Donovan
David M
DM
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D004357:Drug Synergism; D010450:Endopeptidases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008248:Lysostaphin / Q000494:pharmacology*; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000188:drug therapy; D013204:Staphylococcus Phages / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects*; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D010450:Endopeptidases; D008248:Lysostaphin; C001143:endolysin