title: [Authentication of Pinellia pedatisecta and their adulterants by AS-PCR].
abstract: OBJECTIVE
  To establish a molecular method for the authentication of Pinellia pedatisecta and its adulterants.
  
  
  METHODS
  DNA sequences of some species from P. tenore, Typhonium and Arisaema were downloaded from GenBank, the sequences were aligned using DNAMAN. Allele-specific primers for P. pedatisecta and P. tenore were designed according to their SNPs in rpl 20 sequence. The designed primers were used to amplify 10 samples of P. pedatisecta, P. ternata and T. flagelliforme.
  
  
  RESULTS
  A 351 bp band was amplified from P. pedatisecta but not form P. ternata and T. flagelliforme by primer Pprpl149F and Pprpl484R. A 630 bp band was amplified from P. ternate and P. pedatisecta but not from T. flagelliforme by primer Ptrpl94F and Ptrpl699R.
  
  
  CONCLUSIONS
  AS-PCR has the advantages of highly specific and good reproducibility, by which P. pedatisecta can be identified from part of its adulterants quickly. It is a potential method to be used in the molecular identification of other materia medica.
authors: Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700, China.
authors: Guang-Hong
authors: GH
authors: Cui
authors: Xiao-Jing
authors: XJ
authors: Tang
authors: Lu-Qi
authors: LQ
authors: Huang
authors: Zhong-Zhi
authors: ZZ
authors: Qian
mesh_terms: D000483:Alleles; D002681:China; D003257:Consumer Product Safety; D017931:DNA Primers / Q000235:genetics; D030021:Pinellia / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D011786:Quality Control
chemical_list: D017931:DNA Primers