title: Loss of Drop1 expression already at early tumor stages in a wide range of human carcinomas.
abstract: In a study on gene deregulation in ovarian carcinoma we found a mRNA coding for a 350 kDa protein, Drop1, to be downregulated 20- to 180-fold in the majority of ovarian and mammary carcinomas. The mRNA is encoded by a set of exons in the 5' region of the SYNE1 gene. Immunohistochemical staining for Drop1 protein by a specific monoclonal antibody corresponds to the pattern seen for the mRNA. cDNA arrays of matched pairs of tumor and normal tissue and in situ hybridizations confirmed the drastic loss of Drop1 mRNA as a common feature in uterus, cervix, kidney, lung, thyroid and pancreas carcinomas, already at early tumor stages and in all metastases. Two-hybrid studies suggest a role of this deficiency in the malignant progression of epithelial tumors.
authors: Department of Obstetrics and Gynecology, University of Heidelberg, Heidelberg, Germany.
authors: Alexander
authors: A
authors: Marmé
authors: Hans-Peter
authors: HP
authors: Zimmermann
authors: Gerhard
authors: G
authors: Moldenhauer
authors: Marina
authors: M
authors: Schorpp-Kistner
authors: Claudia
authors: C
authors: Müller
authors: Olga
authors: O
authors: Keberlein
authors: Antje
authors: A
authors: Giersch
authors: Jürgen
authors: J
authors: Kretschmer
authors: Brigitte
authors: B
authors: Seib
authors: Eberhard
authors: E
authors: Spiess
authors: Andreas
authors: A
authors: Hunziker
authors: Faustino
authors: F
authors: Merchán
authors: Peter
authors: P
authors: Möller
authors: Uwe
authors: U
authors: Hahn
authors: Raffael
authors: R
authors: Kurek
authors: Frederik
authors: F
authors: Marmé
authors: Gunther
authors: G
authors: Bastert
authors: Diethelm
authors: D
authors: Wallwiener
authors: Herwig
authors: H
authors: Ponstingl
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins; D005091:Exons; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009367:Neoplasm Staging; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020798:Two-Hybrid System Techniques
chemical_list: D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C415465:SYNE1 protein, human