Human cytochromes P450 in the metabolism of drugs: new molecular models of enzyme-substrate interactions.
The overall predictive ability of molecular modelling, as applied to the cytochrome P450 (CYP) system, is analysed in the light of current developments in a variety of techniques, including X-ray crystallography, molecular biology, enzyme kinetics, molecular mechanics and dynamics, in relation to its relevance to drug metabolism in humans. This review demonstrates that it is possible to generate realistic models for the major human CYPs, which metabolise xenobiotics that compare favourably with crystal structures, and thus may be used to derive substrate binding energies that agree closely with experimental K(m) values obtained from enzyme kinetics.
Lewis
David Fv
DF
University of Surrey, Centre for Toxicology, Faculty of Health and Medical Sciences, Guildford, Surrey, UK. d.Lewis@surrey.ac.uk
Ito
Yuko
Y
D001665:Binding Sites; D018360:Crystallography, X-Ray; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011485:Protein Binding; D015262:Xenobiotics / Q000378:metabolism
D004364:Pharmaceutical Preparations; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System