title: Hairy2 functions through both DNA-binding and non DNA-binding mechanisms at the neural plate border in Xenopus.
abstract: The Xenopus helix-loop-helix transcription factor Hairy2 is essential for neural crest progenitor survival and maintains cells in a mitotic undifferentiated pre-neural crest state. However, its mode of action remains largely unknown. Here we show that a Hairy2 DNA-binding mutant is unable to promote cell survival and to upregulate the expression of early neural border genes but is capable to increase cell proliferation and to expand NC in late embryos. We found that Hairy2 transiently activates in a DNA-binding independent manner the expression of the Notch ligand Delta1 and that Delta1 is required for Hairy2 to promote cell proliferation and to expand NC. Finally, we provide evidence that Hairy2 induces Delta1 through the transcription factor Stat3. Together, these results suggest that Hairy2 has a dual mode of action and may function at the neural plate border through both a DNA-binding and a non-DNA-binding Stat3-Delta1 mediated mechanism.
authors: Laboratoire d'Embryologie Moléculaire, Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires (IBMM), Gosselies, Belgium.
authors: Massimo
authors: M
authors: Nichane
authors: Xi
authors: X
authors: Ren
authors: Jacob
authors: J
authors: Souopgui
authors: Eric J
authors: EJ
authors: Bellefroid
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000502:physiology*; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D051796:Inhibitor of Differentiation Proteins / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000378:metabolism; D054258:Neural Plate / Q000196:embryology* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015854:Up-Regulation; D014981:Xenopus / Q000196:embryology* / Q000378:metabolism; D029867:Xenopus Proteins / Q000378:metabolism / Q000502:physiology*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D051796:Inhibitor of Differentiation Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D050796:STAT3 Transcription Factor; D029867:Xenopus Proteins; C439928:HES4 protein, Xenopus; C501325:Id3 protein, Xenopus; C064045:delta protein