Radioimmunoassay for autoantibodies against interferon omega; its use in the diagnosis of autoimmune polyendocrine syndrome type I.
Patients with the autoimmune polyendocrine syndrome I (APS I) have high titers of neutralizing IgG autoantibodies against type I interferons (IFNs), in particular IFN-omega. Until now, the most specific assay has been the antiviral interferon neutralizing assay (AVINA), which has the drawbacks of requiring a cytolytic virus, being cumbersome and difficult to standardise. We have developed a fast and reliable immunoassay based on radiolabelled IFN-omega for quantifying anti-IFN-omega antibodies. Sera from 48 APS I patients were analysed together with those from 5 control groups. All sera from APS I patients were positive for anti-IFN-omega, while, except one serum, all sera from the controls were negative. This method has the advantage over bioassays that it is readily adapted to high throughput. It provides an alternative, sensitive and specific diagnostic test for APS I, and an ideal screening tool to precede mutational analyses of the AIRE gene in suspected APS I cases.
Oftedal
Bergithe E
BE
Section of Endocrinology, Institute of Medicine, University of Bergen, Bergen, Norway. Bergithe.oftedal@med.uib.no
Wolff
Anette S Bøe
AS
Bratland
Eirik
E
Kämpe
Olle
O
Perheentupa
Jaakko
J
Myhre
Anne Grethe
AG
Meager
Anthony
A
Purushothaman
Radhika
R
Ten
Svetlana
S
Husebye
Eystein S
ES
D001323:Autoantibodies / Q000097:blood*; D005260:Female; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D008297:Male; D016884:Polyendocrinopathies, Autoimmune / Q000175:diagnosis* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D013577:Syndrome
D001323:Autoantibodies; D007370:Interferon Type I; C063743:interferon omega 1