Transcription factor WRKY70 displays important but no indispensable roles in jasmonate and salicylic acid signaling.
The transcription factor WRKY70 was previously reported to be a common component in salicylic acid (SA) and jasmonate (JA) mediated signal pathways in Arabidopsis. Here, we present that the inactivation of the WRKY70 gene in wrky70-1 mutant does not alter the responses of both JA and SA, and that wrky70 mutation is unable to restore the coi1 mutant in JA responses. However, overexpression of WRKY70 reduces JA responses such as expression of JA-induced genes and JA-inhibitory root growth, and activates expression of SA-inducible PR1. These data indicate that the WRKY70 is important but not indispensable for JA and SA signaling, and that other regulators may display the redundant role with WRKY70 in modulation of JA and SA responses in Arabidopsis. Furthermore, we showed that JA inhibits expression of WRKY70 and PR1 by both COI1-dependent and COI1-independent pathways.
Ren
Chun-Mei
CM
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Zhu
Qi
Q
Gao
Bi-Da
BD
Ke
Shao-Ying
SY
Yu
Wan-Cong
WC
Xie
Dao-Xin
DX
Peng
Wen
W
D017360:Arabidopsis / Q000187:drug effects* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D003517:Cyclopentanes / Q000494:pharmacology*; D004269:DNA, Bacterial; D005298:Fertility / Q000187:drug effects; D035264:Flowers / Q000187:drug effects / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D016254:Mutagenesis, Insertional; D009154:Mutation / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology*; D020156:Salicylic Acid / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; D004269:DNA, Bacterial; C105496:NPR1 protein, Arabidopsis; D054883:Oxylipins; C036483:T-DNA; D014157:Transcription Factors; C487183:WRKY70 protein, Arabidopsis; C011006:jasmonic acid; D020156:Salicylic Acid