title: Herpes viruses in periodontal compromised sites: comparison between HIV-positive and -negative patients.
abstract: OBJECTIVE
  The objective of this study was to compare the frequency of herpes simplex virus type 1 (HSV-1), Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) in subgingival plaque, saliva and peripheral blood of HIV-positive and-negative patients with periodontal disease.
  
  
  METHODS
  Fifty HIV-positive subjects (23 with gingivitis, 27 with periodontitis) and 50 healthy HIV-negative patients with chronic periodontitis were included in the study. Parameters of probing depth (PD), clinical attachment level (CAL), gingival index and plaque index were recorded. The samples were processed for viral identification by the nested polymerase chain reaction technique.
  
  
  RESULTS
  HCMV was the most prevalent virus in HIV-positive (82%) and-negative patients (84%), and the detection in the three samples was similar (p>0.05). HSV-1 was the least prevalent virus in both groups, being detected in similar frequencies in oral sites and in peripheral blood. EBV-1 was found more frequently in saliva and subgingival plaque of HIV-positive patients than in HIV-negative patients (p< or =0.05).
  
  
  CONCLUSIONS
  EBV-1 was more frequently recovered in oral sites of HIV-positive patients than in HIV-negative patients.
authors: Department of Periodontology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
authors: Sabrina Rosa
authors: SR
authors: Grande
authors: Ana Vitória
authors: AV
authors: Imbronito
authors: Osmar Shizuo
authors: OS
authors: Okuda
authors: Roberto Fraga Moreira
authors: RF
authors: Lotufo
authors: Marina Helena Gallottini
authors: MH
authors: Magalhães
authors: Fabio Daumas
authors: FD
authors: Nunes
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000097:blood / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D003773:Dental Plaque / Q000821:virology; D005260:Female; D005891:Gingivitis / Q000097:blood / Q000150:complications / Q000821:virology*; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000097:blood / Q000150:complications* / Q000821:virology; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D010518:Periodontitis / Q000097:blood / Q000150:complications / Q000821:virology*; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012463:Saliva / Q000821:virology; D018709:Statistics, Nonparametric
chemical_list: D004279:DNA, Viral