Development of nanofiber-covered stents using electrospinning: in vitro and acute phase in vivo experiments.
There are some technical difficulties in treating for a broad necked aneurysm and a higher incidence of recurrence. Because of these drawbacks, more innovative techniques for superior endovascular reconstructive treatment are required. We developed a novel covered stent employing electrospinning to deposit fine polyurethane (PU) fibers onto stents. An in vitro water leak test was designed and applied prior to animal testing to estimate the performance of covered stents and to determine the appropriate amount of PU fibers on a stent. Two tenths of a milligram of PU fibers proved to be sufficient to prevent water leakage. Then, the efficacy of the covered stents to that of bare stents was compared using 10 rabbits in which model aneurysms had been formed at the right common carotid artery by the elastase method. Angiographic evaluation on day 1 posttreatment (acute phase) revealed complete occlusion of the aneurysms and the patency of the parent arteries in animals treated with covered stents. At 10 days poststenting (subacute phase), the aneurysm neck was completely covered with neointimal layer as shown by scanning electron microscopic examination. The PU-covered stent holds promise as a device for treating cerebral aneurysms.
Kuraishi
Keita
K
Department of Neurosurgery, Mie University, Mie, Japan. kuraishikeita@yahoo.co.jp
Iwata
Hiroo
H
Nakano
Shigeyuki
S
Kubota
Shinichiro
S
Tonami
Hiroyuki
H
Toda
Mitsuaki
M
Toma
Naoki
N
Matsushima
Satoshi
S
Hamada
Kazuhide
K
Ogawa
Satoru
S
Taki
Waro
W
D000783:Aneurysm / Q000628:therapy*; D000792:Angiography / Q000379:methods; D000818:Animals; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries / Q000473:pathology; D002340:Carotid Artery Diseases / Q000473:pathology / Q000628:therapy*; D004563:Electrochemistry / Q000379:methods; D002532:Intracranial Aneurysm / Q000473:pathology*; D008670:Metals / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D011817:Rabbits; D015607:Stents; D016896:Treatment Outcome
D008670:Metals; D011140:Polyurethanes