Maintenance of mitochondrial DNA by the Caenorhabditis elegans ATR checkpoint protein ATL-1.
Here we show that inactivation of the ATR-related kinase ATL-1 results in a significant reduction in mitochondrial DNA (mtDNA) copy numbers in Caenorhabditis elegans. Although ribonucleotide reductase (RNR) expression and the ATP/dATP ratio remained unaltered in atl-1 deletion mutants, inhibition of RNR by RNAi or hydroxyurea treatment caused further reductions in mtDNA copy number. These results suggest that ATL-1 functions to maintain mtDNA independently of RNR.
Mori
Chihiro
C
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai 980-8577, Japan.
Takanami
Takako
T
Higashitani
Atsushi
A
D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D002875:Chromosomes; D004272:DNA, Mitochondrial / Q000235:genetics; D017353:Gene Deletion; D006720:Homozygote; D006918:Hydroxyurea / Q000494:pharmacology; D008954:Models, Biological; D008957:Models, Genetic; D009154:Mutation; D010770:Phosphotransferases / Q000235:genetics*; D034622:RNA Interference; D012264:Ribonucleotide Reductases / Q000096:biosynthesis / Q000235:genetics
D029742:Caenorhabditis elegans Proteins; D004272:DNA, Mitochondrial; D000255:Adenosine Triphosphate; D012264:Ribonucleotide Reductases; C576885:Atl-1 protein, C elegans; D010770:Phosphotransferases; D064007:Ataxia Telangiectasia Mutated Proteins; D006918:Hydroxyurea