title: Fine epitope mapping of humanized anti-IgE monoclonal antibody omalizumab.
abstract: Omalizumab is a humanized anti-IgE antibody that inhibits IgE binding to its receptors on mast cells and basophils, thus blocking the IgE-mediated release of pharmacologic mediators from these cells. Previous studies have indicated that omalizumab binds to the Cepsilon3 domain of IgE, which is the binding site of IgE receptors, but the precise epitope recognized by omalizumab is unknown. In this study, we employed the phage display peptide library technology to select peptides binding to omalizumab. A striking peptide sequence motif was recovered, which is homologous to the sequence (424)HLP(426) within the Cepsilon3 domain of IgE-Fc. Our results further indicated that omalizumab specifically bound to the synthesized peptide "(421)THPHLPRALMRS(432)" containing the (424)HLP(426) motif in IgE-Fc. We therefore conclude that the (424)HLP(426) motif is the omalizumab epitope. This epitope overlaps with the high-affinity IgE receptor-binding site, thus providing insights into the structural basis for the mechanism of action of omalizumab.
authors: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, People's Republic of China.
authors: Lei
authors: L
authors: Zheng
authors: Bohua
authors: B
authors: Li
authors: Weizhu
authors: W
authors: Qian
authors: Lei
authors: L
authors: Zhao
authors: Zhiguo
authors: Z
authors: Cao
authors: Shu
authors: S
authors: Shi
authors: Jie
authors: J
authors: Gao
authors: Dapeng
authors: D
authors: Zhang
authors: Sheng
authors: S
authors: Hou
authors: Jianxin
authors: J
authors: Dai
authors: Hao
authors: H
authors: Wang
authors: Yajun
authors: Y
authors: Guo
mesh_terms: D020816:Amino Acid Motifs; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal / Q000276:immunology*; D061067:Antibodies, Monoclonal, Humanized; D018604:Epitope Mapping; D006801:Humans; D007073:Immunoglobulin E / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D000069444:Omalizumab; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011487:Protein Conformation
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D007141:Immunoglobulin Fc Fragments; D019151:Peptide Library; D010455:Peptides; D000069444:Omalizumab; D007073:Immunoglobulin E