Changes in PDGF concentration in surgically treated colorectal carcinoma.
OBJECTIVE
The aim of the present study was to asses the effect of tumor advancement, differentiation grade and surgery treatment on PDGF- AB level and platelet (PLT) count depending on the site of blood collection.


METHODS
The study included 38 patients submitted to surgical treatment due to diagnosis of colorectal cancer (CRC) without remote metastasis: G2- 20 patients and group G3- 18 patients. The control group consisted of 24 healthy subjects. In CRC patients the blood samples was collected three times: 1) before surgery, 2) intrasurgically and 3) 90 days after surgery. Serum PDGF- AB concentration was determined by ELISA- Kit reagents.


RESULTS
PDGF concentration in all the patients was several times higher than in the control group, irrespective of tumor differentiation grade and the site of blood collection. However the level of PDGF- AB in intraoperatively collected arterial blood and venous blood in group G3 (arterial blood- 379.9+/-12.1 ng/ml; venous blood- 398.4+/-13.2 ng/ml) was significantly higher than in group G2 (arterial blood- 169.4+/-88.6 ng/ml; venous blood- 194.2+/-84.0 ng/ml). No significant differences were observed between venous and arterial blood. No correlation was found between the PLT count and PDGF- AB concentration.


CONCLUSIONS
High blood PDGF- AB concentration in CRC patients but no significant positive correlation observed between the PLT count and PDGF-AB suggest its neoplastic origin beside PLT. Determination of this factor in blood serum may have an important implication in early diagnosis of CRC, which is the second most common malignant neoplasm with high recurrence rates.
Mantur
M
M
Department of Clinical Laboratory Diagnostics, Medical University of Bialystok. mantur@umwb.edu.pl
Snarska
J
J
Sidorska
A
A
Ostrowska
H
H
Kruszewska-Wnorowska
K
K
Wojszel
J
J
D000230:Adenocarcinoma / Q000097:blood* / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D001158:Arteries; D014408:Biomarkers, Tumor / Q000097:blood*; D001800:Blood Specimen Collection / Q000379:methods*; D015179:Colorectal Neoplasms / Q000097:blood* / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D014680:Veins
D014408:Biomarkers, Tumor; D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A