title: Effects of imatinib mesylate on normal bone marrow cells from chronic myeloid leukemia patients in complete cytogenetic response.
abstract: Information on the effects of imatinib mesylate (IM) on the non-clonal bone marrow (BM) cell compartment is scanty. We have analyzed the gene expression profile of BM hematopoietic cells after IM therapy in 20 patients with chronic myeloid leukaemia (CML) in complete cytogenetic response (CCyR) and compared it with that of normal volunteer donors by oligonucleotide microarrays. In CCyR CML samples, IM induces a decrease in proliferation as well as increase in apoptosis and ubiquitination in residual non-clonal BM cells. In addition, IM diminishes cell-to-cell adhesion and downregulates the expression of the erythropoietin (EPO) receptor gene. The latter was confirmed by RT-PCR.
authors: Servicio de Hematologia, Hospital Universitario de Salamanca, Salamanca, Spain.
authors: Fermin M
authors: FM
authors: Sanchez-Guijo
authors: Jesus M
authors: JM
authors: Hernandez
authors: Eva
authors: E
authors: Lumbreras
authors: Patricia
authors: P
authors: Morais
authors: Carlos
authors: C
authors: Santamaría
authors: Juan-Luis
authors: JL
authors: Garcia
authors: Norma C
authors: NC
authors: Gutierrez
authors: Jesus F San
authors: JF
authors: Miguel
authors: Maria-Consuelo
authors: MC
authors: Del Cañizo
mesh_terms: D001483:Base Sequence; D001549:Benzamides; D001854:Bone Marrow Cells / Q000187:drug effects*; D017931:DNA Primers; D020869:Gene Expression Profiling; D006801:Humans; D000068877:Imatinib Mesylate; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D010879:Piperazines / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D017467:Receptors, Erythropoietin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001549:Benzamides; D017931:DNA Primers; D010879:Piperazines; D011743:Pyrimidines; D017467:Receptors, Erythropoietin; D000068877:Imatinib Mesylate