[Isolation and identification of lipopeptides produced by Bacillus subtilis using high performance liquid chromatography and electrospray ionization mass spectrometry].
Bacillus subtilis JA produces a broad spectrum of bioactive lipopeptides with great potential applications in agriculture and industry. Crude lipopeptides were extracted with 100% methanol from the precipitate which was obtained by adding 6 mol/L HCl to the cell-free culture broth. The crude extract was separated on a Diamonsil C18 column (5 microm, 250 mm x 4.6 mm) in reversed-phase high performance liquid chromatography (HPLC) system to separate the lipopeptide homologues. Five peaks were eluted from HPLC. Electrospray ionization mass spectrometry (ESI-MS) was used to analyze each HPLC fraction. The results showed three series of ion peaks. According to the values of m/z, the three series of ions were classified into surfactin, iturin and fengycin homologues, which were well-known biosurfactants produced by B. subtilis strains. The major ions were structurally characterized using tandem mass spectrometry. This study suggested a reasonable method for the isolation and identification of lipopeptides produced by B. subtilis strains.
Chen
Hua
H
Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei 230031, China. chenhua0563@yahoo.com.cn
Wang
Li
L
Yuan
Chengling
C
Zheng
Zhiming
Z
Yu
Zengliang
Z
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055666:Lipopeptides / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D055666:Lipopeptides