Anti-inflammatory action of Cudrania tricuspidata on spleen cell and T lymphocyte proliferation.
This study examined whether an extract of Cudrania tricuspidata shows anti-proliferative effects in anti-CD3/CD28-mediated spleen and CD4+CD25- T cells and decreases the production of the proinflammatory cytokines interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) in anti-CD3/CD28-mediated CD4+CD25- T cells. The proliferation of anti-CD3/CD28-mediated spleen cells and CD4+CD25- T cells was effectively suppressed by C. tricuspidata. This extract, however, did not show cytotoxicity in spleen cells under conditions where the antigen was not stimulated using CCK-8 analysis. C. tricuspidata also decreased the production of the pro-inflammatory cytokines IL-2 and IFN-gamma by selective inhibition of this extract on proliferating cells in anti-CD3/CD28-mediated CD4+CD25- T cells. These results suggest that C. tricuspidata may be useful in the treatment of autoimmune diseases and organ transplantation through the inhibitory action of T cells in inflammation.
Chang
Sung Ho
SH
Department of Surgery and Asan Institute for Life Sciences, Ulsan University College of Medicine & Asan Medical Center, Poognapdong, Songpa-gu, Seoul 138-736, Korea.
Jung
Eun Jung
EJ
Lim
Dong Gyun
DG
Oyungerel
Baatartsogt
B
Lim
Kwan Il
KI
Her
Erk
E
Choi
Wahn Soo
WS
Jun
Myung Ha
MH
Choi
Kang Duk
KD
Han
Duck Jong
DJ
Kim
Song Cheol
SC
D000818:Animals; D018106:CD28 Antigens / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D007371:Interferon-gamma / Q000187:drug effects / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D029586:Moraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000378:metabolism
D018106:CD28 Antigens; D017252:CD3 Complex; D007376:Interleukin-2; D010936:Plant Extracts; D007371:Interferon-gamma