Effect of equal daily doses achieved by different power densities of low-level laser therapy at 635 nm on open skin wound healing in normal and corticosteroid-treated rats.
Optimal parameters of low-level laser therapy (LLLT) for wound healing are still discussed. Hence, our study was aimed to compare effects of different power densities of LLLT at 635 nm in rats. Four, round, full-thickness, skin wounds were made on the backs of 48 rats that were divided into two groups (non-steroid laser-treated and steroid laser-treated). Three wounds were stimulated daily with a diode laser (daily dose 5 J/cm(2)) each with different power density (1 mW/cm(2), 5 mW/cm(2), and 15 mW/cm(2)), whereas the fourth wound served as a control. Two days, 6 days, and 14 days after surgery, eight animals from each group were killed and samples were removed for histological evaluation. In the non-steroid laser-treated rats, significant acceleration of epithelization and collagen synthesis 2 days and 6 days after surgery was observed in stimulated wounds. In steroid laser-treated rats, 2 days and 14 days after surgery, a decreased leucocyte/macrophage ratio and a reduction in the area of granulation tissue were recorded, respectively. In conclusion, LLLT, by the method we used, improved wound healing in the non-steroid laser-treated rats, but it was useless after corticosteroid treatment.
Gál
Peter
P
Department of Medical Biophysics, Pavol Jozef Safárik University, Kosice, Slovak Republic. galovci@yahoo.com
Mokrý
Michal
M
Vidinský
Boris
B
Kilík
Róbert
R
Depta
Filip
F
Harakalová
Magdaléna
M
Longauer
Frantisek
F
Mozes
Stefan
S
Sabo
Ján
J
D000818:Animals; D005938:Glucocorticoids / Q000627:therapeutic use; D006097:Granulation Tissue / Q000473:pathology; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D007962:Leukocytes / Q000473:pathology; D028022:Low-Level Light Therapy / Q000379:methods*; D008264:Macrophages / Q000473:pathology; D008297:Male; D008775:Methylprednisolone / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects / Q000293:injuries* / Q000473:pathology / Q000528:radiation effects; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects* / Q000528:radiation effects*
D005938:Glucocorticoids; D008775:Methylprednisolone