title: The influence of mineral trioxide aggregate on adaptive immune responses to endodontic pathogens in mice.
abstract: This study assessed the influence of mineral trioxide aggregate (MTA) on adaptive immune responses. BALB/c mice were immunized with heat-killed Fusobacterium nucleatum (Fn) in MTA or other control adjuvants, and serum IgG responses to Fn were measured. Either Fn- or Peptostreptococcus anaerobius (Pa)-reactive memory T cells (Tm) were preincubated in vitro with/without MTA and restimulated with Fn or Pa. Tm proliferation and cytokine production were assessed. Compared with control groups, immunoglobulin G-antibody responses were upregulated in mice immunized with Fn in MTA in a similar manner to animals immunized with Fn in Freund's adjuvant or aluminum hydroxide adjuvant. Although MTA did not affect the upregulated expression of interleukin 10, tumor necrosis factor alpha, or RANKL by Tm, it suppressed the proliferation of Pa- or Fn-Tm and inhibited their production of Th1- or Th2-signature cytokines. MTA upregulated the adaptive humoral immune responses but had little or no effect on pro- or anti-inflammatory cytokine production by Tm.
authors: Departamento de Dentística Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Taia Maria Berto
authors: TM
authors: Rezende
authors: Leda Quercia
authors: LQ
authors: Vieira
authors: Antônio Paulino Ribeiro
authors: AP
authors: Sobrinho
authors: Ricardo Reis
authors: RR
authors: Oliveira
authors: Martin A
authors: MA
authors: Taubman
authors: Toshihisa
authors: T
authors: Kawai
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D000917:Antibody Formation / Q000187:drug effects*; D000942:Antigens, Bacterial / Q000276:immunology; D017610:Calcium Compounds / Q000494:pharmacology*; D004338:Drug Combinations; D016967:Fusobacterium nucleatum / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007156:Immunologic Memory / Q000187:drug effects*; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010087:Oxides / Q000494:pharmacology*; D010462:Peptostreptococcus / Q000276:immunology; D053245:RANK Ligand / Q000096:biosynthesis; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D017607:Aluminum Compounds; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D017610:Calcium Compounds; D004338:Drug Combinations; D007074:Immunoglobulin G; D010087:Oxides; D053245:RANK Ligand; D012387:Root Canal Filling Materials; D017640:Silicates; D014409:Tumor Necrosis Factor-alpha; C086631:mineral trioxide aggregate; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma