X-ray induced damage in DNA monitored by X-ray photoelectron spectroscopy.
In this work, the chemical changes in calf thymus DNA samples were analyzed by X-ray photoelectron spectroscopy (XPS). The DNA samples were irradiated for over 5 h and spectra were taken repeatedly every 30 min. In this approach the X-ray beam both damages and probes the samples. In most cases, XPS spectra have complex shapes due to contributions of C, N, and O atoms bonded at several different sites. We show that from a comparative analysis of the modification in XPS line shapes of the C 1s, O 1s, N 1s, and P 2p peaks, one can gain insight into a number of reaction pathways leading to radiation damage to DNA.
Ptasińska
Sylwia
S
Department of Physics and Astronomy, The Open University, Walton Hall, Milton Keynes, MK7 6AA, United Kingdom. s.ptasinska@open.ac.uk
Stypczyńska
Agnieszka
A
Nixon
Tony
T
Mason
Nigel J
NJ
Klyachko
Dimitri V
DV
Sanche
Léon
L
D000818:Animals; D002417:Cattle; D004247:DNA / Q000235:genetics; D004249:DNA Damage / Q000528:radiation effects*; D013057:Spectrum Analysis; D014965:X-Rays
D004247:DNA; C427203:calf thymus DNA