[Effects of desensitization of capsaicin-sensitive afferent neurons on gastric microcirculation in rats depend on the blood glucocorticoid hormone level].
The effects of desensitization of capsaicin-sensitive afferent neurons on gastric microcirculation were investigated before and after administration of indomethacin at ulcerogenic dose in adrenalectomized rats with or without corticosterone replacement and in sham-operated animals. We estimated the blood flow velocity in submucosal microvessels; the diameters and permeability of mucosal venous microvessels as parameters of gastric microcirculation. Desensitization of capsaicin-sensitive neurons was performed with capsaicin at the dose 100 mg/kg two weeks before the experiment. Adrenalectomy was created one week before experiment. In vivo microscopy technique for the direct visualization of gastric microcirculation and the analysis of the blood flow was employed. Indomethacin at ulcerogenic dose decreased the blood flow velocity in submucosal microvessels, caused dilatation of superficial mucosal microvessels and increased their permeability. Desensitization of capsaicin-sensitive afferent neurons potentiated indomethacin-induced microvascular disturbances in gastric submucosa-mucosa. These potentiated effects of the desensitization are obviously promoted by concomitant glucocorticoid deficiency. Thus, glucocorticoid hormones have a beneficial effect on gastric microcirculation in rats with desensitization of capsaicin-sensitive afferent neurons.
Podvigina
T T
TT
Bobryshev
P Iu
PIu
Bagaeva
T R
TR
Mal'tsev
N A
NA
Levkovich
Iu I
IuI
Filaretova
L P
LP
D000315:Adrenalectomy; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects; D001783:Blood Flow Velocity; D002199:Capillary Permeability; D002211:Capsaicin / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000098:blood supply / Q000294:innervation; D005938:Glucocorticoids / Q000097:blood*; D007213:Indomethacin / Q000009:adverse effects; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D009475:Neurons, Afferent / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000098:blood supply* / Q000294:innervation
D000894:Anti-Inflammatory Agents, Non-Steroidal; D005938:Glucocorticoids; D002211:Capsaicin; D007213:Indomethacin