A latex agglutination assay for specific detection of Panton-Valentine leukocidin.
Panton-Valentine leukocidin (PVL) is produced by some isolates of Staphylococcus aureus, and has been associated with the high pathogenic potential of these strains. To rapidly detect the toxin producer strains, we developed a reverse passive latex agglutination (RPLA) reaction assay specific for PVL. By testing 64 S. aureus strains, the assay could detect the 35 pvl-gene-positive strains with 100% specificity and sensitivity. Furthermore, the assay revealed an extensive variation in the amount of PVL produced by the pvl-positive strains.
Oishi
Kanenari
K
Bacterial Diagnostics Production Department, Denka Seiken Co. Ltd., Gosen, Niigata, Japan.
Baba
Tadashi
T
Nakatomi
Yasuo
Y
Ito
Teruyo
T
Hiramatsu
Keiichi
K
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D005098:Exotoxins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D007956:Leukocidins / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin