title: Analysis of the association of preeclampsia with polymorphisms of the INS, INSR and IRS1 genes in Mexican women.
abstract: OBJECTIVE
  It has been proposed that preeclampsia is a metabolic syndrome of pregnancy. The polymorphisms PstI and MaeIII of INS, NsiI of INSR and Ala513Pro and Gly972Arg of IRS1 have been associated with metabolic syndrome; moreover, the products of these genes are functionally contiguous during insulin signaling. The aim of this study was to assess whether these polymorphisms are associated with preeclampsia.
  
  
  METHODS
  46 normotensive pregnant women and 43 preeclamptic patients were included in the study to develop a clinical, biochemical and genotypic profile of preeclampsia. Clinical evaluation consisted of measurement of blood pressure, height and weight. Peripheral blood samples were collected for determination of fasting glucose and insulin concentrations and for extraction of genomic DNA. Proteinuria was determined. Polymorphisms were detected using PCR-RFLP.
  
  
  RESULTS
  The normotensive and preeclampsia groups did not differ significantly in clinical and biochemical traits, except for systolic and diastolic blood pressure (p < 0.0001). Polymorphisms previously associated with metabolic syndrome in Mexican populations were not associated with preeclampsia in Mexican women (p > 0.05).
  
  
  CONCLUSIONS
  The lack of an association between preeclampsia and the polymorphisms studied suggests that other genes whose products do not have direct functional interaction with metabolic syndrome or epigenetic factors may play a role in preeclampsia.
authors: División de Medicina Molecular, CIBO, IMSS, Guadalajara, Mexico.
authors: Maria-Victoria
authors: MV
authors: Machorro-Lazo
authors: Jose
authors: J
authors: Sanchez-Corona
authors: Esperanza
authors: E
authors: Martínez-Abundis
authors: Manuel
authors: M
authors: González-Ortiz
authors: Carlos
authors: C
authors: Galaviz-Hernandez
authors: Francisco-Javier
authors: FJ
authors: Perea
authors: Alejandra-Guadalupe
authors: AG
authors: Garcia-Zapien
authors: Edhit-Guadalupe
authors: EG
authors: Cruz-Quevedo
authors: Lorenzo
authors: L
authors: Salgado-Goytia
authors: Maria-Cristina
authors: MC
authors: Moran-Moguel
authors: Silvia-Esperanza
authors: SE
authors: Flores-Martínez
mesh_terms: D000328:Adult; D000483:Alleles; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D003430:Cross-Sectional Studies; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005260:Female; D006239:Haplotypes; D006801:Humans; D007328:Insulin / Q000097:blood / Q000235:genetics*; D055504:Insulin Receptor Substrate Proteins / Q000097:blood / Q000235:genetics*; D008800:Mexico; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000097:blood / Q000235:genetics*; D011247:Pregnancy; D011972:Receptor, Insulin / Q000097:blood / Q000235:genetics*; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; D004247:DNA; D011972:Receptor, Insulin