Discrimination of repetitive sequences polymorphism in Secale cereale by genomic in situ hybridization-banding.
Genomic in situ hybridization banding (GISH-banding), a technique slightly modified from conventional GISH, was used to probe the Chinese native rye (Secale cereale L.) DNA, and enabled us to visualize the individual rye chromosomes and create a universal reference karyotype of the S. cereale chromosome 1R to 7R. The GISH-banding approach used in the present study was able to discriminate S. cereale chromosomes or segments in the wheat (Triticum aestivum L.) background, including the Triticale, wheat-rye addition and translocation lines. Moreover, the GISH-banding pattern of S. cereale subsp. Afghanicum chromosomes was consistent with that of Chinese native rye cv. Jingzhou rye; whereas the GISH-banding pattern of Secale vavilovii was different from that of S. cereale, indicating that GISH-banding can be used to study evolutionary polymorphism in species or subspecies of Secale. In addition, the production and application of GISH-banding to the study of adenine-thymine-riched heterochromatin is discussed.
Zhou
Jian-Ping
JP
Shcool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Yang
Zu-Jun
ZJ
Li
Guang-Rong
GR
Liu
Cheng
C
Ren
Zheng-Long
ZL
D002871:Chromosome Banding; D032461:Chromosomes, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D017403:In Situ Hybridization; D008938:Mitosis; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012434:Secale / Q000166:cytology / Q000235:genetics*