[Rapid determination of alkaloids in tobacco using gas chromatography-mass spectrometry with full scan-selected ion monitoring mode].
Simultaneous full scan-selected ion monitoring mode (Scan-SIM) is a new improvement method of data acquisition of gas chromatography-mass spectrometry (GC-MS). A method of determing alkaloids in tobacco was established by GC-MS with Scan-SIM. Nicotine, nornicotine, anabasine and anatabine were determined with scan mode, and myosmine, nicotyrine, 2,3'-bipyridyl and cotinine were determined with SIM mode. The average recoveries of alkaloids ranged from 94.8% to 98.8%, and the relative standard deviations were less than 6.0% (n = 5). Therefore, it is a simple, rapid, accurate method. Tobacco samples picked in different years were determined using this method, and the results were satisfactory.
Wang
Baoxing
B
Yunnan Reascend Tobacco Technology (Group) Co., Ltd, Kunming 650106, China. bxwang@reascend.com.cn
Yang
Shihua
S
Hou
Ying
Y
Zeng
Xiaoying
X
Wu
Yi
Y
Xu
Guowang
G
D000470:Alkaloids / Q000032:analysis*; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D016014:Linear Models; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D014026:Nicotiana / Q000737:chemistry*
D000470:Alkaloids; D012997:Solvents