title: Dynamic expression patterns of G protein-regulated inducer of neurite outgrowth 1 (GRIN1) and its colocalization with Galphao implicate significant roles of Galphao-GRIN1 signaling in nervous system.
abstract: GRIN1 (Gprin 1) is a signaling molecule coexpression of which with constitutively active form of Galphao can stimulate neurite extensions in Neuro2a cells, yet its in vivo roles remain elusive. Here, we examine expression profiles of GRIN1 during mouse development by in situ hybridization (ISH) and immunohistochemistry. ISH analysis revealed that GRIN1 expression was limited to the nervous system at all developmental stages tested: in the central nervous system, GRIN1 expression occurred within the entire embryonic mantle zones, while it became restricted to sets of nuclei at postnatal to adult stages. Immunohistochemistry using a GRIN1-specific antibody demonstrated that GRIN1 colocalized with Galphao at neuronal dendrites and axons, but it was not detected in glial cells. These results suggest that Galphao-GRIN1 pathway could mediate significant roles in neuronal migration and differentiation at embryonic stages and exert functions in wiring and/or maintenance of specific neural circuitries at postnatal to adult stages.
authors: Department of Pharmacology, College of Medicine, University of Illinois at Chicago, Illinois 60612, USA.
authors: Ikuo
authors: I
authors: Masuho
authors: Yasumasa
authors: Y
authors: Mototani
authors: Yoshinori
authors: Y
authors: Sahara
authors: Junko
authors: J
authors: Asami
authors: Shun
authors: S
authors: Nakamura
authors: Tohru
authors: T
authors: Kozasa
authors: Takayoshi
authors: T
authors: Inoue
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009420:Nervous System / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D017933:Peripheral Nervous System / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D044385:GTP-Binding Protein alpha Subunits; C121673:Gprin1 protein, mouse; D009419:Nerve Tissue Proteins; D016194:Receptors, N-Methyl-D-Aspartate; C084618:olfactory G protein subunit alpha olf