Determination of microRNAs in mouse preimplantation embryos by microarray.
Expression profile of microRNA (miRNA) in mouse oocytes and preimplantation embryos has been revealed by a novel high throughput microarray assay. A total of 97 (43 "new" and 54 known) including mouse, human, and predicted miRNAs have been discovered in the preimplantation mouse embryos which can be classified into developmental stage-dependent groups and non-stage-dependent group according to the statistical analysis of the expression patterns. Potential gene targets of each group of miRNAs are estimated by TargetsScan system and significantly changed signaling pathways and biological processes underlying these gene targets are searched by PANTHER classification system between the stage-dependent miRNAs and the non-stage-dependent miRNAs. Expression of some miRNAs is confirmed by reverse transcriptase-polymerase chain reaction. It is shown that dynamic synthesis and degradation of miRNAs coexists in the preimplantation development of mouse embryos. However, the overall quantity of miRNAs and percentage of the stage-dependent miRNAs increase as the preimplantation embryos develop.
Yang
Yanhong
Y
Department of Obstetrics & Gynaecology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
Bai
Wentao
W
Zhang
Liang
L
Yin
Guowu
G
Wang
Xiaohong
X
Wang
Jun
J
Zhao
Hongxi
H
Han
Yibing
Y
Yao
Yuan-Qing
YQ
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D035683:MicroRNAs