Chondrogenic differentiation of mouse embryonic stem cells promoted by mature chondrocytes.
In order to direct embryonic stem (ES) cells to differentiate into chondrocytes, a chondrogenic environment provided by mature chondrocytes was investigated. Flk-1 positive cells sorted from pre-differentiated mouse ES cells were mixed with adult porcine articular chondrocytes, seeded on biodegradable scaffolds, and then implanted subcutaneously into nude mice. The cell-scaffold complexes formed cartilage tissues after 4 weeks, which was demonstrated by histology and anti-type II collagen antibody staining. Positive staining of mouse Major Histocompatibility Complex class I molecules confirmed that part of the chondrocytes were derived from mouse ES cells. The current study established a new approach for directing ES cell differentiation.
Xie
Feng
F
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai, 200011, China.
Zhang
WenJie
W
Chen
FanFan
F
Zhou
GuangDong
G
Cui
Lei
L
Liu
Wei
W
Cao
YiLin
Y
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology*; D020219:Chondrogenesis / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D033581:Stem Cell Transplantation; D013552:Swine; D013724:Teratoma / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
D040301:Vascular Endothelial Growth Factor Receptor-2