[A molecular view of microbial diversity in sediment of the Jinshan hot spring, Guangdong province].
OBJECTIVE
The aim of this study is to analyze the microbial diversity in the sediment of Jingshan hot spring.


METHODS
We extracted environment DNA from the sediment and then amplified the 16S rRNA genes of prokarotype and internal transcribed spacers (ITS) DNA of eukarotype by PCR with specific conservative primers. The PCR products were ligated to the T vectors and transformed into Escherichia coli to construct libraries. The libraries were analyzed by amplified ribosomal DNA restriction analysis (ARDRA) approach. The clones of dominant ARDRA patterns were selected to sequence. The sequence similarities were analyzed by using the BLAST programs for searching the GenBank DNA databases.


RESULTS
We identified 14 dominant operational taxonomic units (OTUs) from prokarotype G. group. The Phylogenetic tree shows that 7 of them (JS-GU20, JS-GU1, JS-G37, JS-G29, JS-GM2, JS-G42, JS-G41) belong to Bdellovibrio. They had the highest similarity to Bacteriovorax sp. NE1 (EF092445) and Bdellovibrio sp. JS5 (AF084859), 96% and 99% respectively. Four major OTUs of prokarotype X group belong to Cyanobacterium. One of them, named JS-X2, has a similarity of 95% with an uncultured Cyanobacterium (L35331) which was gained from a hot spring of the Yellowstone National Park and has a similarity of 89% with Thermosynechococcus elongatus BP-1 (47118315). Most OTUs of eukaryotic group have 90% sequences similarity to that of Penicillium sp..


CONCLUSIONS
The results showed that the microbial diversity in the sediment of Jinshan hot spring is rich. And the result of several clones' similarity to Bacteriovorax sp. showed that there might be a new group of thermophilicBacteriovorax sp. in the hot spring sediment.
Xiao
Kai
K
State Key Laboratory for BioControl, College of Life Sciences, Sun Yat-sen, Zhongshan, University, Guangzhou 510275, China. coolegg12@yahoo.com.cn
Cao
Lixiang
L
Lu
Yongjun
Y
Zhou
Shining
S
D044822:Biodiversity; D002681:China; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D045482:Hot Springs / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S