title: NS5ATP9 gene regulated by NF-kappaB signal pathway.
abstract: NS5ATP9 was previously identified as p15 PAF [proliferating cell nuclear antigen (PCNA)-associated factor] to bind with PCNA. We earlier identified the promoter region of NS5ATP9 and found NS5ATP9 is a NS5A up-regulation gene. However little is known about how it is regulated. To investigate the gene regulation of NS5ATP9, we screened NS5ATP9 promoter binding proteins using phage display and verified by electrophoretic mobility shift assay (EMSA). We found that the nuclear protein rhNF-kappaB (p50) could bind to the NS5ATP9 promoter and the binding region contained within a 156 bp (nucleotides -5 to -161bp) immediately upstream of the transcription initiation site. Our results suggest that NF-kappaB could participate in the regulation of NS5ATP9 gene expression in carcinogenesis.
authors: The First Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors: Kang
authors: K
authors: Li
authors: Qingyong
authors: Q
authors: Ma
authors: Lei
authors: L
authors: Shi
authors: Chengxue
authors: C
authors: Dang
authors: Yuan
authors: Y
authors: Hong
authors: Qi
authors: Q
authors: Wang
authors: Yue
authors: Y
authors: Li
authors: Wanhu
authors: W
authors: Fan
authors: Lei
authors: L
authors: Zhang
authors: Jun
authors: J
authors: Cheng
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D019295:Computational Biology / Q000379:methods; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation / Q000502:physiology*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D019151:Peptide Library; D011401:Promoter Regions, Genetic; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D016328:NF-kappa B; C425208:PCLAF protein, human; D019151:Peptide Library