Rapid determination of nitazoxanide in tablets using reversed-phase ultra-performance liquid chromatography (UPLC) and high-performance liquid chromatography.
A simple and rapid determination method for nitazoxanide (NTZ), an antiprotozoal agent, was developed using reverse-phase HPLC and Ultra Performance Liquid Chromatography (UPLC). Only six minutes gradient condition for NTZ analysis using UPLC was achieved. The mobile phase consisted of a mixture of phosphate buffer (pH 6.0) and acetonitrile. The repeatability (relative standard deviation (RSD), n = 6) and the correlation coefficient from linearity (the range from 80% to 120% of amount) were 0.25% and 0.9963 for UPLC and 0.15% and 0.9988 for HPLC, respectively. The quantitative values of NTZ in tablets were 103.2% for HPLC and 98.7% for UPLC. The RSDs of quantitative values of sample solution were calculated to be 4.06% to 4.64% for HPLC and 0.15% to 0.36% for UPLC.
Sakamoto
T
T
Division of Drugs, National Institute of Health Sciences, Tokyo, Japan. tsakamot@nihs.go.jp
Hiyama
Y
Y
D000465:Algorithms; D000981:Antiprotozoal Agents / Q000032:analysis* / Q000302:isolation & purification; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D009574:Nitro Compounds; D019999:Pharmaceutical Solutions; D012015:Reference Standards; D015203:Reproducibility of Results; D013607:Tablets; D013844:Thiazoles / Q000032:analysis* / Q000302:isolation & purification
D000981:Antiprotozoal Agents; D009574:Nitro Compounds; D019999:Pharmaceutical Solutions; D013607:Tablets; D013844:Thiazoles; C041747:nitazoxanide