title: Production of C5a by ASP, a serine protease released from Aeromonas sobria.
abstract: Aeromonas sobria causes pus and edema at sites of infection. However, the mechanisms underlying these effects have not been elucidated. C5a, the amino-terminal fragment of the complement 5th component (C5), mimics these events. To investigate the involvement of C5a in the pathophysiology of A. sobria infection, we examined release of C5a from human C5 by a serine protease (ASP), a putative virulence factor secreted by this bacterium. C5 incubated with enzymatically active ASP induced neutrophil migration in a dose-dependent manner from an ASP concentration of 3 nM and in an incubation time-dependent manner in as little as 7 min, with neutrophil accumulation in guinea pigs at intradermal injection sites and neutrophil superoxide release. These effects on neutrophils were inhibited by a C5a-receptor antagonist. The ASP incubation mixture with C5 but not C3 elicited vascular leakage in a dose- and incubation time-dependent manner, which was inhibited by a histamine H(1)-receptor antagonist. Together with these C5a-like activities, ASP cleaved C5 to release only one C5a Ag, the m.w. of which was similar to that of C5a. Immunoblotting using an anti-C5a Ab revealed generation of a C5a-like fragment from human plasma incubated with ASP. These results suggest that ASP-elicited neutrophil migration and vascular leakage via C5a production from C5 could occur in vivo, which was supported by that ASP did not affect functions of C5a and neutrophil C5a receptor. Through C5a generation, ASP could be associated with the induction of pus and edema caused by infection with this bacterium.
authors: Department of Molecular Pathology, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan.
authors: Hidetoshi
authors: H
authors: Nitta
authors: Takahisa
authors: T
authors: Imamura
authors: Yoshihiro
authors: Y
authors: Wada
authors: Atsushi
authors: A
authors: Irie
authors: Hidetomo
authors: H
authors: Kobayashi
authors: Keinosuke
authors: K
authors: Okamoto
authors: Hideo
authors: H
authors: Baba
mesh_terms: D000333:Aeromonas / Q000201:enzymology / Q000472:pathogenicity*; D000818:Animals; D001426:Bacterial Proteins; D002199:Capillary Permeability; D002634:Chemotaxis, Leukocyte; D003182:Complement C5 / Q000378:metabolism; D015936:Complement C5a; D016905:Gram-Negative Bacterial Infections / Q000473:pathology; D006168:Guinea Pigs; D006801:Humans; D009504:Neutrophils; D012697:Serine Endopeptidases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003182:Complement C5; D015936:Complement C5a; D012697:Serine Endopeptidases