CdTe nanocrystal-based electrochemical biosensor for the recognition of neutravidin by anodic stripping voltammetry at electrodeposited bismuth film.
CdTe quantum dots (QDs)-based electrochemical sensor for recognition of neutravidin, as a model protein, using anodic stripping voltammetry at electrodeposited bismuth film is presented. This biosensor involves the immobilization of the captured QDs conjugates which was dissolved with 1M HCl solution to release cadmium ions and metal components were quantified by anodic stripping voltammetry after a 3-min accumulation at -1.2V on bismuth-film electrode (BiFE) of the biotin, served as recognition element, onto the gold surface in connection with a cysteamine self-assembled monolayer. The modification procedure was characterized by electrochemical impedance spectroscopy and atomic force microscopy. We exploit QDs as labels for amplifying signal output and monitoring the extent of competition process between CdTe-labeled neutravidin and the target neutravidin for the limited binding sites on biotin. As expected for the competitive mechanism, the recognition event thus yields distinct cadmium stripping voltammetric current peak, whose response decreases upon increasing the level of target neutravidin concentrations. Under optimal conditions, the voltammetric response is highly linear over the range of 0.5-100 ngL(-1) neutravidin and the limit of detection is estimated to be 0.3 ngL(-1) (5 nM). Unlike earlier two-step sandwich bioassays, the present protocol relies on a one-step competitive assay, which is more accurate and sensitive, showing great promise for rapid, simple and cost-effective analysis of protein.
Du
Dan
D
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China.
Ding
Jiawang
J
Tao
Yuan
Y
Li
Haibing
H
Chen
Xi
X
D001360:Avidin / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001729:Bismuth / Q000737:chemistry*; D019187:Cadmium Compounds / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation*; D004595:Electroplating; D004867:Equipment Design; D019544:Equipment Failure Analysis; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013691:Tellurium / Q000737:chemistry*
D019187:Cadmium Compounds; C093483:neutravidin; D001360:Avidin; D013691:Tellurium; C028337:cadmium telluride; D001729:Bismuth