Evidences for complex formation between L-dabPNA and aegPNA.
Continuing our research on the development of nucleopeptides as ODN analogs for biomedical and bioengineering applications, here we report the synthesis and the chemical-physical characterization of a homoadenine hexamer based on a l-diaminobutyric acid (l-DABA) backbone (dabPNA), and its binding studies with a complementary aegPNA. We demonstrated by CD and UV experiments that the l-dabPNA binds the aegPNA forming a complex with good thermal stability, that we identified as a left-handed triplex.
Roviello
Giovanni N
GN
Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, I-80134 Napoli, Italy.
Musumeci
Domenica
D
Bucci
Enrico M
EM
Castiglione
Mariangela
M
Cesarani
Annalisa
A
Pedone
Carlo
C
Piccialli
Gennaro
G
D000227:Adenine Nucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D000613:Aminobutyrates / Q000737:chemistry* / Q000378:metabolism*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015195:Drug Design; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
D000227:Adenine Nucleotides; D000613:Aminobutyrates; D020135:Peptide Nucleic Acids; C527043:aminoethylglycyl PNA; C051802:triplex DNA; D004247:DNA; D005998:Glycine