Electron tomography shows molecular anchoring within a layer-by-layer film.
The layer-by-layer self-assembly of thin films consisting of alternating layers of DNA and bis-urea nanoribbons prevents diffusion of the components within the film and allows the anchoring of biotinylated molecules through molecular recognition in a predetermined layer of the film. Electron tomography demonstrates with nanometer precision the location of gold-labeled streptavidin bound to the incorporated biotinylated molecules.
Vos
Matthijn R J
MR
Soft Matter Cryo-TEM Research Unit, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
Breurken
Monica
M
Leclère
Philippe E L G
PE
Bomans
Paul H H
PH
de Haas
Felix
F
Frederik
Peter M
PM
Jansen
John A
JA
Nolte
Roeland J M
RJ
Sommerdijk
Nico A J M
NA
D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D055032:Electron Microscope Tomography / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D014508:Urea / Q000737:chemistry*
D013501:Surface-Active Agents; D001710:Biotin; D014508:Urea; D004247:DNA; D019809:Streptavidin