Variables that affect platelet function analyzer-100 (PFA-100) closure times and establishment of reference intervals in Korean adults.
The PFA-100 is an in vitro system for measurement of platelet function in whole blood. To establish reference intervals in the Korean population, we investigated the relationships between PFA-100 closure times and gender, ABO blood group, citrate concentration, and time of blood collection in 120 well-characterized healthy Korean adults. Gender did not affect closure time values. Blood group O was associated with longer collagen/epinephrine (CEPI) and collagen/ADP (CADP) closure times than non-O groups (p <0.0001 for both). Closure times were shorter in samples obtained in the morning vs the afternoon. Regression analysis showed an association between CEPI and CADP closure times (p <0.0001), but there were no associations between PFA-100 results and WBC count, hemoglobin, hematocrit, platelet count, and platelet-associated parameters. The reference intervals for CEPI and CADP closure times of 3.2% citrated blood were 80-162 and 64-121 sec, respectively. The reference intervals for PFA-100 closure times in healthy Korean adults were similar to those in Western populations. Flow obstruction was observed in 31 of 480 (6.5%) measurements, but routine performance of duplicate measurements was otherwise unnecessary because of acceptable CV values. PFA-100 closure time should be interpreted with consideration of the patient's clinical presentation and factors such as ABO blood group and diurnal variation.
Cho
Young-Uk
YU
Department of Laboratory Medicine, University of Ulsan, College of Medicine, and Asan Medical Center, Seoul, Korea.
Jang
Seongsoo
S
Park
Chan-Jeoung
CJ
Chi
Hyun-Sook
HS
D000328:Adult; D005260:Female; D006801:Humans; D007723:Korea; D008297:Male; D010979:Platelet Function Tests / Q000295:instrumentation*; D012016:Reference Values; D013997:Time Factors