Potential therapy of Fc-antigen combination-encoding DNA vaccination in mouse allergic airway inflammation.
Vaccination with allergen-encoding DNA has been proposed as having potential for allergen-specific immunotherapy. In this study, we examine the therapeutic effect of allergen-encoding DNA vaccination directly to dendritic cells (DCs) on allergen-induced allergic airway inflammation in a mouse model and explore potential mechanism. Ovalbumin (OVA)-sensitized and challenged mice were immunized with DNA vaccine and received bronchoalveolar lavage (BAL) 1 day after the last challenge, to measure BAL levels of interleukin (IL)-4, IL-5, interferon (IFN)-gamma and differential cell count. Pulmonary DCs and Spleen DCs were purified and sorted according to the expression of CD(11c) (+)CD(80) (+) and CD(11c) (+)CD(86) (+) co-stimulatory molecules. Our data demonstrated that DNA vaccine therapy with OVA-Fc-pcDNA(3.1) significantly prevented OVA-increased levels of IL-4, IL-5 and the percentage of eosinophils and OVA-decreased level of IFN-gamma. OVA-Fc-pcDNA(3.1)-treated mice had less severity of airway inflammation, and lower expression of CD(11c) (+)CD(80) (+) and CD(11c) (+)CD(86) (+) on pulmonary DCs, as compared with animals with OVA-pcDNA(3.1,) pcDNA(3.1) and OVA respectively. DNA vaccine encoding both Fc and OVA was shown to be more effective than DNA vaccine encoding OVA alone. Our data indicate that Fc-antigen combination-encoding DNA vaccination has better preventive effects on antigen-induced airway inflammation by regulating DCs, and may be a new alternative therapy for asthma.
Wang
Y
Y
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Qian
G
G
Wang
G
G
Cheng
X
X
Bai
C
C
Wang
X
X
D000818:Animals; D001249:Asthma / Q000276:immunology / Q000628:therapy*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology; D005434:Flow Cytometry; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000097:blood; D007141:Immunoglobulin Fc Fragments / Q000235:genetics*; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000032:analysis; D015848:Interleukin-5 / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000235:genetics; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
D007141:Immunoglobulin Fc Fragments; D015848:Interleukin-5; D019444:Vaccines, DNA; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin