title: Long-term durability, tissue regeneration and neo-organ growth during skeletal maturation with a neo-bladder augmentation construct.
abstract: OBJECTIVE
  To comparatively evaluate bladder regeneration following 80% cystectomy and augmentation using a synthetic biopolymer with autologous urothelial and smooth muscle cells (autologous neo-bladder augmentation construct [construct]) or autotransplantation of native bladder (reimplanted native urinary bladder [reimplant]) in canines.
  
  
  METHODS
  Voiding function, urodynamic assessment and neo-organ capacity-to-body-weight ratio (C:BW) were assessed longitudinally for a total of 24 months following trigone-sparing augmentation cystoplasty in juvenile canines.
  
  
  RESULTS
  Within 30 days postimplantation, hematology and urinalysis returned to baseline. Constructs and reimplants yielded neo-organs with statistically equivalent urodynamics and histology. Linear regression analysis of C:BW showed that constructs regained baseline slope and continued to adapt with animal growth.
  
  
  CONCLUSIONS
  Constructs and reimplants regained and maintained native bladder histology by 3 months, capacity at 3-6 months and compliance by 12-24 months. Furthermore, construct C:BW demonstrated the ability of regenerated bladder to respond to growth regulation.
authors: Tengion, Inc., 3929 Westpoint Blvd., Suite G, Winston-Salem, NC 27103, USA.
authors: Manuel J
authors: MJ
authors: Jayo
authors: Deepak
authors: D
authors: Jain
authors: John W
authors: JW
authors: Ludlow
authors: Richard
authors: R
authors: Payne
authors: Belinda J
authors: BJ
authors: Wagner
authors: Gordon
authors: G
authors: McLorie
authors: Timothy A
authors: TA
authors: Bertram
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001704:Biopolymers / Q000737:chemistry; D017690:Cell Transplantation; D004285:Dogs; D005260:Female; D008297:Male; D008954:Models, Biological; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D011108:Polymers / Q000737:chemistry; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods; D001743:Urinary Bladder / Q000473:pathology* / Q000502:physiology; D014563:Urodynamics
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D011108:Polymers