Cellular and humoral immune responses during intrathoracic paracoccidioidomycosis in BALB/c mice.
Paracoccidioidomycosis is a chronic infection that primarily affects the lungs. Here we investigated cellular and humoral immune responses after intrathoracic Paracoccidioidesbrasiliensis infection in BALB/c mice. P. brasiliensis-colony-forming units (CFUs), fungal DNA and granulomas in lungs increased progressively, peaking at day 90 postinfection (p.i.). IFN-gamma production was highest on day 15 p.i., declining thereafter. The kinetics of the NO production was similar to that described for IFN-gamma. In contrast, IL-10 increased from day 45 p.i. reaching a peak at day 90. Levels of serum IgG1 were higher than IgG2a between days 30 and 90 p.i. 30% of mice died by day 90 p.i. These data indicate that infection with P. brasiliensis by the intrathoracic route shows high IFN-gamma and NO production at day 15 p.i., unable to control multiplication of fungi, which appears to be associated with a progressive increase in IL-10 and in the number and complexity of granulomas.
Alves
Caio C S
CC
Laboratory of Immunology, Department of Parasitology, Microbiology and Immunology, Biological Sciences Institute, Federal University of Juiz de Fora, 36033-330 Juiz de Fora, Minas Gerais, Brazil.
Azevedo
Ana L S
AL
Rodrigues
Michele F
MF
Machado
Rachel P
RP
Souza
Maria A
MA
Machado
Marco A
MA
Teixeira
Henrique C
HC
Ferreira
Ana P
AP
D000818:Animals; D000908:Antibodies, Fungal; D000917:Antibody Formation; D006099:Granuloma / Q000382:microbiology / Q000473:pathology; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D010228:Paracoccidioides / Q000276:immunology / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D013909:Thorax / Q000382:microbiology* / Q000473:pathology
D000908:Antibodies, Fungal; D007074:Immunoglobulin G; D016753:Interleukin-10; D009569:Nitric Oxide; D007371:Interferon-gamma