title: Purification and characterisation of a panel of peanut allergens suitable for use in allergy diagnosis.
abstract: Peanut is a major cause of type 1 hypersensitive reactions including anaphylaxis. This results from the presence of a number of protein allergens, six of which are being studied as part of the EU FP6 EuroPrevall programme. These are Ara h 1 (7S globulin), Ara h 2, Ara h 6 (2S albumins), Ara h 3/4 (11S globulins) and Ara h 8 (Bet v 1 homologue). Methods for the purification of Ara h 1, Ara h 3/4, Ara h 2 and Ara h 6 from peanut seeds and for the production of recombinant Ara h 8 in Escherichia coli are described with spectroscopic analyses being used to confirm that they are authentically folded. N-terminal sequencing of the proteins purified from peanut seeds also revealed details of the differences between isoforms and their generation by proteolytic processing within the seed. Preliminary IgE binding studies of the purified allergens confirmed that they retained their immunological properties indicating their suitability for use in allergy diagnosis.
authors: Rothamsted Research, Harpenden, Hertfordshire, UK.
authors: Justin
authors: J
authors: Marsh
authors: Neil
authors: N
authors: Rigby
authors: Klaus
authors: K
authors: Wellner
authors: Gerald
authors: G
authors: Reese
authors: André
authors: A
authors: Knulst
authors: Jaap
authors: J
authors: Akkerdaas
authors: Ronald
authors: R
authors: van Ree
authors: Christian
authors: C
authors: Radauer
authors: Alison
authors: A
authors: Lovegrove
authors: Ana
authors: A
authors: Sancho
authors: Clare
authors: C
authors: Mills
authors: Stefan
authors: S
authors: Vieths
authors: Karin
authors: K
authors: Hoffmann-Sommergruber
authors: Peter R
authors: PR
authors: Shewry
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D010367:Arachis / Q000276:immunology*; D001483:Base Sequence; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008969:Molecular Sequence Data; D021183:Peanut Hypersensitivity / Q000175:diagnosis*; D017433:Protein Structure, Secondary
chemical_list: D000485:Allergens; D007073:Immunoglobulin E