Expression pattern and functional analysis of a MADS-box gene M79 from rice.
Using a degenerated primer and a T-primer, a MADS-box gene, M79, was amplified by RT-PCR from rice fluorescence at meiosis stage and then cloned. Sequence analysis shows that M79 shares 98.2% homology with OsMADS7 at DNA level while only 92% at the amino acid level. The transcript of M79 possesses five different polyadenylation sites. Only a single copy of M79 gene has been found in rice genome, which is located on chromosome 8. M79 is expressed specifically in flower organs, from pre-meiosis stage through pollen maturation. Ectopic expression of M79 in T(0) and T(1) transgenic rice results in early-flowering, implying that M79 is involved in controlling the flowering time. In the same time, M79 may be involved in controlling the branching process to make more flower buds.
Qu
L
L
Peking University, Beijing, China.
Zhang
Y
Y
Li
C
C
Liu
M
M
Wang
J
J
Huang
B
B
Li
Z
Z
Wang
S
S
Gu
H
H
Chen
Z
Z