Elucidation of the interactions of an anticancer ruthenium complex in clinical trials with biomolecules utilizing capillary electrophoresis hyphenated to inductively coupled plasma-mass spectrometry. Short communication.
The application of capillary electrophoresis (CE) combined with highly sensitive inductively-coupled-plasma mass spectrometric (ICP-MS) detection allows the interactions of metal complexes with biomolecules to be characterized. This technique has been used to provide new insights into the mode of action of the ruthenium-based anticancer drug candidate indazolium [trans-tetrachlorobis(1H-indazole)ruthenate(III)] (KP1019). While the compound binds rapidly and efficiently to serum proteins, especially albumin, its reactivity towards the model DNA compound 2'-deoxyguanosine 5'-monophosphate (5'-dGMP) is moderate.
Groessl
Michael
M
University of Vienna, Institute of Inorganic Chemistry, Waehringer Strasse 42, A-1090 Vienna.
Hartinger
Christian G
CG
University of Vienna, Institute of Inorganic Chemistry, Waehringer Strasse 42, A-1090 Vienna.|Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne.
Połeć-Pawlak
Kasia
K
Chair of Analytical Chemistry, Warsaw University of Technology, ul. Noakowskiego 3, PL-00-664 Warsaw.
Jarosz
Maciej
M
Chair of Analytical Chemistry, Warsaw University of Technology, ul. Noakowskiego 3, PL-00-664 Warsaw.
Dyson
Paul J
PJ
Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne.
Keppler
Bernhard K
BK
University of Vienna, Institute of Inorganic Chemistry, Waehringer Strasse 42, A-1090 Vienna.
D000970:Antineoplastic Agents / Q000097:blood / Q000737:chemistry* / Q000494:pharmacology; D001665:Binding Sites; D002986:Clinical Trials as Topic; D004247:DNA / Q000737:chemistry; D003848:Deoxyguanine Nucleotides / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007191:Indazoles / Q000097:blood / Q000737:chemistry* / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods*; D008968:Molecular Conformation; D009942:Organometallic Compounds; D017975:Ruthenium Compounds / Q000097:blood / Q000737:chemistry* / Q000494:pharmacology; D012709:Serum Albumin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
D000970:Antineoplastic Agents; D003848:Deoxyguanine Nucleotides; D007191:Indazoles; D009942:Organometallic Compounds; D017975:Ruthenium Compounds; D012709:Serum Albumin; C499292:indazolium trans-(tetrachlorobis(1H-indazole)ruthenate (III)); C007257:2'-deoxyguanosine 5'-phosphate; D004247:DNA