Long-term persistence of a single Legionella pneumophila strain possessing the mip gene in a municipal shower despite repeated cycles of chlorination.
The ability of Legionella pneumophila to colonise domestic water systems is a primary cause of outbreaks of Legionnaire's disease in humans. World Health Organization guidelines recommend that drinking water is chlorinated to between 0.2 and 1mg/L [Chlorine in drinking-water. Guidelines for drinking-water quality, 2nd edn. Geneva: World Health Organization; 1996], but L. pneumophila is repeatedly isolated from chlorinated water systems, indicating that this treatment is not effective at preventing colonisation. Current UK guidelines recommend a one-off treatment of 20-50mg/L of free chlorine to remove the bacteria. In this study we report on the persistence of L. pneumophila serogroup 1 in a domestic shower system despite repeated cycles of chlorination at 50mg/L for 1h exposure time, over the course of two and a half years. Persisting isolates were subjected to in-vitro phenotypic analyses and polymerase chain reaction analysis for the toxin-encoding mip gene. Random amplified polymorphic DNA typing was also performed to determine whether the isolates recovered on different occasions were the same strain. We found that seven isolates of L. pneumophila recovered over a two-and-a-half year period are the same genetically defined strain, indicating that the bacteria can persist despite repeated cycles of chlorination after each successive isolation.
Cooper
I R
IR
School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK. i.cooper@bton.ac.uk
White
J
J
Mahenthiralingam
E
E
Hanlon
G W
GW
D001426:Bacterial Proteins / Q000235:genetics*; D002713:Chlorine / Q000494:pharmacology*; D002947:Cities; D016172:DNA Fingerprinting / Q000379:methods; D004203:Disinfection / Q000379:methods; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016952:Legionella pneumophila / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D019696:Peptidylprolyl Isomerase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D014871:Water Microbiology; D014881:Water Supply
D001426:Bacterial Proteins; D002713:Chlorine; C512922:Mip protein, Legionella pneumophila; D019696:Peptidylprolyl Isomerase