Dynamics of neutrophil migration in lymph nodes during infection.
Although the signals that control neutrophil migration from the blood to sites of infection have been well characterized, little is known about their migration patterns within lymph nodes or the strategies that neutrophils use to find their local sites of action. To address these questions, we used two-photon scanning-laser microscopy to examine neutrophil migration in intact lymph nodes during infection with an intracellular parasite, Toxoplasma gondii. We found that neutrophils formed both small, transient and large, persistent swarms via a coordinated migration pattern. We provided evidence that cooperative action of neutrophils and parasite egress from host cells could trigger swarm formation. Neutrophil swarm formation coincided in space and time with the removal of macrophages that line the subcapsular sinus of the lymph node. Our data provide insights into the cellular mechanisms underlying neutrophil swarming and suggest new roles for neutrophils in shaping immune responses.
Chtanova
Tatyana
T
Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, CA 94720, USA.
Schaeffer
Marie
M
Han
Seong-Ji
SJ
van Dooren
Giel G
GG
Nollmann
Marcelo
M
Herzmark
Paul
P
Chan
Shiao Wei
SW
Satija
Harshita
H
Camfield
Kristin
K
Aaron
Holly
H
Striepen
Boris
B
Robey
Ellen A
EA
D000818:Animals; D002465:Cell Movement; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology* / Q000469:parasitology; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000469:parasitology; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*