Bacterial DNA sample preparation from whole blood using surface-modified Si pillar arrays.
A novel bacterial DNA sample preparation device for molecular diagnostics has been developed. On the basis of optimized conditions for bacterial adhesion, surface-modified silicon pillar arrays for bacterial cell capture were fabricated, and their ability to capture bacterial cells was demonstrated. The capture efficiency for bacterial cells such as Escherichia coli, Staphylococcus epidermidis, and Streptococcus mutans in buffer solution was over 75% with a flow rate of 400 microL/min. Moreover, the proposed method captured E. coli cells present in 50% whole blood effectively. The captured cells from whole blood were then in- situ lyzed on the surface of the microchip, and the eluted DNA was successfully amplified by qPCR. These results demonstrate that the full process of pathogen capture to DNA isolation from whole blood could be automated in a single microchip.
Hwang
Kyu-Youn
KY
Bio & Health Lab., Samsung Advanced Institute of Technology (SAIT), Mt. 14-1, Nongseo-Dong, Giheung-Gu, Yongin-Si, Gyeonggi-Do, South Korea, 449-712.
Lim
Hee-Kyun
HK
Jung
Seong-Young
SY
Namkoong
Kak
K
Kim
Joon-Ho
JH
Huh
Nam
N
Ko
Christopher
C
Park
Jae-Chan
JC
D053000:Analytic Sample Preparation Methods / Q000379:methods*; D001419:Bacteria / Q000166:cytology / Q000378:metabolism; D002448:Cell Adhesion; D004269:DNA, Bacterial / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
D004269:DNA, Bacterial; D012825:Silicon