Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging.
Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3+/-0.4 ms were achieved with the single-layer microfluidic device.
Robinson
Tom
T
Chemical Biology Centre, Department of Chemical Engineering, Imperial College London, London, UK.
Valluri
Prashant
P
Manning
Hugh B
HB
Owen
Dylan M
DM
Munro
Ian
I
Talbot
Clifford B
CB
Dunsby
Christopher
C
Eccleston
John F
JF
Baldwin
Geoff S
GS
Neil
Mark A A
MA
de Mello
Andrew J
AJ
French
Paul M W
PM
D002138:Calibration; D004867:Equipment Design; D005453:Fluorescence; D021621:Imaging, Three-Dimensional; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D044085:Microfluidics; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors