A nuclear localized protein ZCCHC9 is expressed in cerebral cortex and suppresses the MAPK signal pathway.
The CCHC-type zinc finger motif has numerous biological activities (such as DNA binding and RNA binding) and can also mediate protein-protein interaction. This article gives a primary report about the human ZCCHC9 gene. Protein ZCCHC9 contains four CCHC motifs and is highly conserved in humans, mice, and rats. The whole cDNA sequence of the ZCCHC9 gene has been amplified by PCR and a number of plasmids have been constructed for further study. The results show that ZCCHC9 is localized in the nucleus, and especially concentrated in the nucleolus. It is highly expressed in the brain and testicles of the mouse. This has been confirmed by real-time reverse transcription polymerase chain reaction (RT-PCR). In situ hybridization of the mouse brain indicates that ZCCHC9 is mainly expressed in the cerebral cortex. Reporter gene assay shows that ZCCHC9 suppresses the transcription activities of NF-kappa B and SRE, and may play roles in the Mitogen-Activated Protein Kinase (MAPK) signaling transduction pathway.
Zhou
Aidong
A
Key Laboratory of Protein Chemistry and Developmental Biology, Ministry of Education of China, College of Life Science, Hunan Normal University, Changsha 410081, China.
Zhou
Jianlin
J
Yang
Liping
L
Liu
Mingjun
M
Li
Hong
H
Xu
Su
S
Han
Mei
M
Zhang
Jian
J
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D016335:Zinc Fingers / Q000235:genetics / Q000502:physiology
C433227:CNBP protein, human; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C558792:ZCCHC9 protein, human; D020928:Mitogen-Activated Protein Kinases