Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains.
An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.
López-Goñi
I
I
Departamento de Microbiología y Parasitología, Universidad de Navarra, c/ Irunlarrea no. 1, 31008 Pamplona, Spain. ilgoni@unav.es
García-Yoldi
D
D
Marín
C M
CM
de Miguel
M J
MJ
Muñoz
P M
PM
Blasco
J M
JM
Jacques
I
I
Grayon
M
M
Cloeckaert
A
A
Ferreira
A C
AC
Cardoso
R
R
Corrêa de Sá
M I
MI
Walravens
K
K
Albert
D
D
Garin-Bastuji
B
B
D000818:Animals; D015373:Bacterial Typing Techniques; D002002:Brucella / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008322:Mammals; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers