Highly expressed dectin-1 on bone marrow-derived dendritic cells regulates the sensitivity to beta-glucan in DBA/2 mice.
Sparassis crispa beta-glucan (SCG) is a major six-branched 1,3-beta-D-glucan in S. crispa Fr. showing antitumor activity. We previously found that DBA/1 and DBA/2 mice are highly sensitive to SCG in vivo and in vitro. In this study, we investigated the effect of SCG on bone marrow-derived dendritic cells (BMDCs) in DBA/2 mice in vitro. SCG increased the expression of CD80, MHC class I and MHC class II molecules on the cell membrane of BMDCs. SCG induced BMDCs to produce interleukin-12p70 (IL-12p70), IL-6, and tumor necrosis factor-alpha (TNF-alpha). The magnitude of cytokine induction by SCG in DBA/2 mice was higher than that in C57BL/6, BALB/c, C3H/HeN, and C3H/HeJ mice. The expression level of the beta-glucan receptor, dectin-1, on BMDCs in DBA/2 mice was also highest in DBA/2 among these mice. Blocking dectin-1 significantly inhibited the induction of TNF-alpha production by SCG. Taken together, these results suggest that the BMDCs from DBA/2 mice are highly sensitive to the induction of cytokine production by SCG in vitro, and that this sensitivity is related to the expression level of dectin-1.
Harada
Toshie
T
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo, Japan.
Miura
Noriko N
NN
Adachi
Yoshiyuki
Y
Nakajima
Mitsuhiro
M
Yadomae
Toshiro
T
Ohno
Naohito
N
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D018122:B7-1 Antigen / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D037181:Lectins, C-Type; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D020072:Polyporales / Q000737:chemistry*; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology*; D000099181:Dectin-1
D000970:Antineoplastic Agents; D018122:B7-1 Antigen; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D018664:Interleukin-12; D015850:Interleukin-6; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D014409:Tumor Necrosis Factor-alpha; D047071:beta-Glucans; D000099181:Dectin-1