title: Mechanotransducers in rat pulpal afferents.
abstract: The hydrodynamic theory suggests that pain associated with stimulation of a sensitive tooth ultimately involves mechanotransduction as a consequence of fluid movement within exposed dentinal tubules. To determine whether putative mechanotransducers could underlie mechanotransduction in pulpal afferents, we used a single-cell PCR approach to screen retrogradely labeled pulpal afferents. The presence of mRNA encoding BNC-1, ASIC3, TRPV4, TRPA1, the alpha, beta, and gamma subunits of ENaC, and the two pore K+ channels (TREK1, TREK2) and TRAAK were screened in pulpal neurons from rats with and without pulpal inflammation. ASIC3, TRPA1, TREK1, and TREK2 were present in approximately 67%, 64%, 14%, and 10% of pulpal neurons, respectively. There was no detectable influence of inflammation on the proportion of neurons expressing these mechanotransducers. Given that the majority of pulpal afferents express ASIC3 and TRPA1, our results raise the possibility that these channels may be novel targets for the treatment of dentin sensitivity.
authors: Program in Neuroscience, University of Maryland, Baltimore, MD 21201, USA.
authors: T O
authors: TO
authors: Hermanstyne
authors: K
authors: K
authors: Markowitz
authors: L
authors: L
authors: Fan
authors: M S
authors: MS
authors: Gold
mesh_terms: D062565:Acid Sensing Ion Channels; D000818:Animals; D003782:Dental Pulp / Q000166:cytology* / Q000294:innervation / Q000378:metabolism; D003789:Dental Pulp Exposure / Q000378:metabolism; D008297:Male; D008437:Maxilla; D008465:Mechanoreceptors / Q000502:physiology*; D008963:Molar; D009412:Nerve Fibers / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009431:Neural Conduction / Q000502:physiology; D009475:Neurons, Afferent / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000166:cytology* / Q000378:metabolism
chemical_list: C568269:ASIC3 protein, rat; D062565:Acid Sensing Ion Channels; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D015222:Sodium Channels