Investigation of immunogenic T-cell epitopes in SARS virus nucleocapsid protein and their role in the prevention and treatment of SARS infection.
1. A novel HLA-A2.1-specific SARS coronavirus (SARS-CoV) nucleocapsid (N) protein epitope (N220-N228 LALLLLDRL) able to activate cytotoxic T cells in vitro has been identified. 2. When used with a single-chain-trimer system, the SARS-CoV N protein epitope (N220-N228 LALLLLDRL) can stimulate a cytotoxic T-cell response against N-protein expressing cells in the HLA-A2.1K(b) transgenic mouse model.
Cheung
Y K
YK
Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Cheng
S C S
SC
Sin
F W Y
FW
Chan
K T
KT
Xie
Y
Y
D000818:Animals; D000086462:Coronavirus Nucleocapsid Proteins; D004279:DNA, Viral / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D019590:Nucleocapsid Proteins / Q000276:immunology*; D011336:Probability; D011897:Random Allocation; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control* / Q000628:therapy*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology / Q000494:pharmacology*
D000086462:Coronavirus Nucleocapsid Proteins; D004279:DNA, Viral; D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; C000706247:N protein, SARS-CoV; D019590:Nucleocapsid Proteins; D019444:Vaccines, DNA; D014765:Viral Vaccines