Gelatin/chitosan/hyaluronan scaffold integrated with PLGA microspheres for cartilage tissue engineering.
Poly(lactide-co-glycotide) (PLGA) microspheres integrated into gelatin/chitosan/hyaluronan scaffolds were fabricated by freeze-drying and crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide. The effects of the microspheres on porosity, density, compressive modulus, phosphate-buffered saline uptake ratio and weight loss of the scaffolds were evaluated. Generally, a scaffold with a higher PLGA content had a lower porosity and weight loss, and a medium uptake ratio, but a larger apparent density and compressive modulus. When the PLGA content was lower than 50%, the PLGA-integrated scaffolds had a similar pore size (approximately 200microm) as that of the control, and as much as 90% of their porosity could be preserved. In vitro chondrocyte culture in the 50% PLGA-integrated scaffold demonstrated that the cells could proliferate and secrete extracellular matrix at the same level as in the control gelatin/chitosan/hyaluronan scaffold.
Tan
Huaping
H
Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of Education, and Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Wu
Jindan
J
Lao
Lihong
L
Gao
Changyou
C
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002356:Cartilage / Q000378:metabolism*; D049109:Cell Proliferation; D048271:Chitosan / Q000008:administration & dosage*; D019902:Chondrocytes / Q000378:metabolism; D019245:Compressive Strength; D005780:Gelatin / Q000008:administration & dosage*; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D019344:Lactic Acid / Q000737:chemistry*; D008863:Microspheres; D008954:Models, Biological; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005780:Gelatin; D006820:Hyaluronic Acid; D048271:Chitosan