title: Human papillomavirus detection and typification in cutaneous and mucosal lesions of HIV-seropositive patients.
abstract: High-risk human papillomavirus (HPV) in a lesion is related to an increased chance of neoplasic transformation, especially when with immunosuppression, as in HIV infection. We investigated HPV frequency in cutaneous and mucosal lesions of HIV-seropositive male patients. The frequency of malignancy, its association with the HPV type detected and some clinical variables were also assessed. A total of 38 lesions from 27 patients were studied in a period of 18 months. The biopsied fragment was submitted to HPV detection and typification, through polymerase chain reaction with generic (MY09/11) and specific (types 6, 11, 16 and 18) primers. HPV frequency was 63.2%, with detection of HPV types 6, 11 or 16 in 18 lesions and with multi-infection in three. There was low detection of high-risk HPV (type 16, 18.4%) and no HPV type 18. Of the lesions, 36.8% were already premalignant or malignant, and the frequency of moderate or severe dysplasia was higher in the study patients than that described in the HIV-seronegative population. High-risk HPV (type 16) was detected in four benign lesions and low-risk HPV (type 6) in three premalignant genital lesions. There was no significant association between the clinical variables and an increase in the prevalence of premalignant or malignant lesions.
authors: Sector of Dermatology and Post Graduation course in Dermatology, Universidade Federal do Rio de Janeiro, Rua Alice 278/402, Rio de Janeiro, Brazil. marianagusmao@bol.com.br
authors: Mariana de Gusmão
authors: Mde G
authors: Nunes
authors: Melissa
authors: M
authors: Azevedo-e-Silva
authors: Carolina Pereira
authors: CP
authors: Gonçalves
authors: Beatriz Moritz
authors: BM
authors: Trope
authors: Ledy do Horto dos Santos
authors: Ldo H
authors: Oliveira
authors: Marcia
authors: M
authors: Ramos-e-Silva
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000235:genetics; D015658:HIV Infections / Q000150:complications*; D006679:HIV Seropositivity; D006801:Humans; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000821:virology; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications* / Q000473:pathology / Q000821:virology*; D014860:Warts / Q000821:virology*
chemical_list: D004279:DNA, Viral