Cytokine IL-1 beta but not IL-1 alpha promoter polymorphism is associated with Alzheimer disease in a population from the Canary Islands, Spain.
OBJECTIVE
Previous studies have reported the presence of low-grade inflammation in Alzheimer disease (AD). Based on these data, our work attempts to investigate the effects of some promoter polymorphisms of pro-inflammatory cytokines [interleukin (IL)-1 alpha and IL-1 beta] on AD.


METHODS
A PCR-RFLP technique was used to analyze the promoter polymorphisms of both IL-1 alpha (-889 C/T) and IL-1 beta (-511 C/T) and the APOE genotype from the DNA samples of 282 patients (according to NINCDS-ADRDA criteria) and 312 control subjects.


RESULTS
(i) The risk of developing AD in our population was associated with the IL-1 beta (-511 C/T) promoter polymorphism; (ii) such risk was independent of the risk factor allele in the APOE gene (APOE4); and (iii) the IL-1 alpha promoter polymorphism (-889 C/T) was not associated with the disease.


CONCLUSIONS
In our population, IL-1 beta promoter polymorphism (-511 C/T) is an independent risk factor for AD.
Déniz-Naranjo
M C
MC
Department of Immunology, Hospital Universitario Dr. Negrín de Gran Canaria, Las Palmas de Gran Canaria, Spain.
Muñoz-Fernandez
C
C
Alemany-Rodríguez
M J
MJ
Pérez-Vieitez
M C
MC
Aladro-Benito
Y
Y
Irurita-Latasa
J
J
Sánchez-García
F
F
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000453:epidemiology / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D053582:Interleukin-1alpha / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013030:Spain / Q000453:epidemiology
D001057:Apolipoproteins E; D053582:Interleukin-1alpha; D053583:Interleukin-1beta