Genetic identification of yeast 18S rRNA residues required for efficient recruitment of initiator tRNA(Met) and AUG selection.
High-resolution structures of bacterial 70S ribosomes have provided atomic details about mRNA and tRNA binding to the decoding center during elongation, but such information is lacking for preinitiation complexes (PICs). We identified residues in yeast 18S rRNA critical in vivo for recruiting methionyl tRNA(i)(Met) to 40S subunits during initiation by isolating mutations that derepress GCN4 mRNA translation. Several such Gcd(-) mutations alter the A928:U1389 base pair in helix 28 (h28) and allow PICs to scan through the start codons of upstream ORFs that normally repress GCN4 translation. The A928U substitution also impairs TC binding to PICs in a reconstituted system in vitro. Mutation of the bulge G926 in h28 and certain other residues corresponding to direct contacts with the P-site codon or tRNA in bacterial 70S complexes confer Gcd(-) phenotypes that (like A928 substitutions) are suppressed by overexpressing tRNA(i)(Met). Hence, the nonconserved 928:1389 base pair in h28, plus conserved 18S rRNA residues corresponding to P-site contacts in bacterial ribosomes, are critical for efficient Met-tRNA(i)(Met) binding and AUG selection in eukaryotes.
Dong
Jinsheng
J
Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Nanda
Jagpreet S
JS
Rahman
Hafsa
H
Pruitt
Margaret R
MR
Shin
Byung-Sik
BS
Wong
Chi-Ming
CM
Lorsch
Jon R
JR
Hinnebusch
Alan G
AG
D019943:Amino Acid Substitution; D018387:Codon, Initiator / Q000235:genetics*; D020717:Eukaryotic Initiation Factor-2B / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010442:Peptide Chain Initiation, Translational; D012331:RNA, Fungal / Q000235:genetics* / Q000378:metabolism; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012358:RNA, Transfer, Met / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
D018387:Codon, Initiator; D020717:Eukaryotic Initiation Factor-2B; C501390:GCN3 protein, S cerevisiae; D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S; D012358:RNA, Transfer, Met; D029701:Saccharomyces cerevisiae Proteins