title: Substantivity of amine fluoride/stannous fluoride following different modes of application: a randomized, investigator-blind, placebo-controlled trial.
abstract: OBJECTIVE
  Amine fluoride/stannous fluoride (ASF) is proven to be effective against plaque and gingivitis. The purpose of this clinical controlled study was to investigate the influence of different application modes on the substantivity of this formulation.
  
  
  METHODS
  Seventeen healthy volunteers received a professional dental prophylaxis. Undisturbed plaque growth was permitted for the next 48 h. In a crossover design, participants received ASF as a single mouthrinse, toothpaste, slurries with high (HA) or low (LA) air content, or a placebo. Vitality of plaque bacteria was investigated before and at 1, 2, 3, 4, 6, and 8h after application of ASF. ANOVA was applied on a 0.05 significance level.
  
  
  RESULTS
  Highest reduction of plaque vitality resulted after toothpaste application, followed by mouthrinse, LA, and HA slurry. No changes occurred in the placebo group. Compared to baseline and placebo, statistically significant changes were detected up to 4h in all ASF groups. Toothpaste exerted antibacterial efficacy up to 8h. Vitality reduction was higher in the LA group than in the HA group.
  
  
  CONCLUSIONS
  The concentration of ASF in formulations influences the time course of the antibacterial effect. Contact of ASF formulations with air might reduce their efficacy.
authors: Department of Conservative Dentistry, Medical Faculty, University of Technology Dresden, Dresden, Germany.
authors: Bernhard
authors: B
authors: Weiland
authors: Lutz
authors: L
authors: Netuschil
authors: Thomas
authors: T
authors: Hoffmann
authors: Katrin
authors: K
authors: Lorenz
mesh_terms: D000328:Adult; D000388:Air; D000588:Amines / Q000008:administration & dosage / Q000494:pharmacology*; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000188:drug therapy* / Q000382:microbiology; D004338:Drug Combinations; D004355:Drug Stability; D005260:Female; D006801:Humans; D008297:Male; D050296:Microbial Viability / Q000187:drug effects*; D009067:Mouthwashes / Q000494:pharmacology*; D000069437:Pharmacological Phenomena; D016037:Single-Blind Method; D014002:Tin Fluorides / Q000008:administration & dosage / Q000494:pharmacology*; D014100:Toothpastes / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000588:Amines; D004338:Drug Combinations; D009067:Mouthwashes; D014002:Tin Fluorides; D014100:Toothpastes; C059813:Meridol