title: Antibacterial protection of suture material by chlorhexidine-functionalized polyelectrolyte multilayer films.
abstract: The formation of bacterial biofilms on the surface of implanted materials is a critical factor that may lead to chronic microbial infection and tissue necrosis. In the present study we analysed the stability of polyelectrolyte multilayer (ML) films on suture materials and the antibacterial effect obtained with chlorhexidine (CHX)-functionalized films built on different types of suture materials such as silk, polyester and copolymer of glycolide and L: -lactide. The comparison of Escherichia coli culture on glass coverslips and glass coverslips with ML and CHX showed at 24 h an inhibition of the bacterial relative luminescence (40.68%, P < 0.5) and at 48 h (99.46%, P < 0.001). In another way, simple soaking of suture material overnight in CHX digluconate 20% without polyelectrolyte films did not at all protect sutures from bacterial colonization but CHX-functionalized polyelectrolyte films, made from poly-L: -glutamic acid and poly-L: -lysine, inhibited Escherichia coli proliferation.
authors: Faculté de chirurgie dentaire, 1 place de l'Hôpital, 67000, Strasbourg, France.
authors: Jean-Claude
authors: JC
authors: Harnet
authors: Erell
authors: E
authors: Le Guen
authors: Vincent
authors: V
authors: Ball
authors: Henri
authors: H
authors: Tenenbaum
authors: Joelle
authors: J
authors: Ogier
authors: Youssef
authors: Y
authors: Haikel
authors: Constant
authors: C
authors: Vodouhê
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002710:Chlorhexidine / Q000737:chemistry* / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004573:Electrolytes / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D008422:Materials Testing; D015394:Molecular Structure; D011099:Polyglutamic Acid / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013537:Sutures
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D011108:Polymers; D011107:Polylysine; D011099:Polyglutamic Acid; D002710:Chlorhexidine