Association of SelS mRNA expression in omental adipose tissue with Homa-IR and serum amyloid A in patients with type 2 diabetes mellitus.
BACKGROUND
Tanis was reported as a putative receptor for serum amyloid A (SAA) involving glucose regulated protein in insulin regulated resistance. It was found to be dysregulated in diabetic rats (Psammomys obesus, Israeli sand rat) and its homologue for humans is SelS/AD-015. The present study analyzed mRNA expression of SelS in omental adipose tissue biopsies from patients with type 2 diabetes mellitus (T2DM), and age- and weight-matched nondiabetic patients, the relationship of SelS mRNA with Homa-IR and serum SAA level.


METHODS
Human omental adipose tissues from ten cases of type 2 diabetic patients and twelve cases of nondiabetic individuals were analyzed for the expression level of SelS mRNA by semiquantitative polymerase chain reaction (PCR), Homa-IR estimated by standard formula and SAA level by enzyme-linked immunosorbent assay (ELISA).


RESULTS
SelS mRNA expression, Homa-IR and serum SAA were higher in T2DM sufferers than in nondiabetic control group. SelS mRNA level was positively correlated with Homa-IR and SAA level in each group.


CONCLUSIONS
SelS protein may be involved in insulin resistance in Chinese with T2DM by acting as the SAA receptor, thus playing an important role in the development of T2DM and atherosclerosis.
Du
Jian-ling
JL
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian, Liaoning, China. dujianling63@163.com
Sun
Chang-kai
CK
Lü
Bo
B
Men
Li-li
LL
Yao
Jun-jie
JJ
An
Li-jia
LJ
Song
Gui-rong
GR
D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000368:Aged; D001483:Base Sequence; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009852:Omentum / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D051140:Selenoproteins / Q000235:genetics*; D000685:Serum Amyloid A Protein / Q000032:analysis*
D008565:Membrane Proteins; D012333:RNA, Messenger; C487710:SELENOS protein, human; D051140:Selenoproteins; D000685:Serum Amyloid A Protein