title: Microsatellite analysis of the GLC1B locus on chromosome 2 points to NCK2 as a new candidate gene for normal tension glaucoma.
abstract: OBJECTIVE
  The aim of this study was to investigate the association between normal tension glaucoma and the candidate disease locus glaucoma 1, open angle, B (GLC1B) on chromosome 2. There are many reports describing the results of association or linkage studies for primary open angle glaucoma (POAG), with GLC1B as one of the loci associated with normal or moderately elevated intraocular pressure. However, there are few reports about the association of genes or defined genomic regions with normal tension glaucoma, which is the leading type of glaucoma in Japan. The GLC1B locus is hypothesized to be a causative region for normal tension glaucoma.
  
  
  METHODS
  Genomic DNA was extracted from whole blood of normal tension glaucoma (n = 143) and healthy controls (n = 103) of Japanese origin.
  
  
  RESULTS
  Fifteen microsatellite markers within and/or near to the GLC1B locus were genotyped, and their association with normal tension glaucoma was analysed. Two markers D2S2264 and D2S176 had significant positive associations.
  
  
  CONCLUSIONS
  The D2S176 marker had the strongest significant association and it is located 24 kb from the nearest gene NCK2, which now becomes an important new candidate gene for future studies of its association with normal tension glaucoma.
authors: Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan.
authors: M
authors: M
authors: Akiyama
authors: K
authors: K
authors: Yatsu
authors: M
authors: M
authors: Ota
authors: Y
authors: Y
authors: Katsuyama
authors: K
authors: K
authors: Kashiwagi
authors: F
authors: F
authors: Mabuchi
authors: H
authors: H
authors: Iijima
authors: K
authors: K
authors: Kawase
authors: T
authors: T
authors: Yamamoto
authors: M
authors: M
authors: Nakamura
authors: A
authors: A
authors: Negi
authors: T
authors: T
authors: Sagara
authors: N
authors: N
authors: Kumagai
authors: T
authors: T
authors: Nishida
authors: M
authors: M
authors: Inatani
authors: H
authors: H
authors: Tanihara
authors: S
authors: S
authors: Ohno
authors: H
authors: H
authors: Inoko
authors: N
authors: N
authors: Mizuki
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000328:Adult; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D004276:DNA, Satellite; D005260:Female; D008040:Genetic Linkage / Q000502:physiology; D005838:Genotype; D005901:Glaucoma / Q000235:genetics; D005902:Glaucoma, Open-Angle / Q000235:genetics*; D006801:Humans; D007429:Intraocular Pressure / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D015513:Oncogene Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004276:DNA, Satellite; C485544:NCK2 protein, human; D015513:Oncogene Proteins