Alteration of brain levels of neurotransmitters and amino acids in male F344 rats induced by three-week repeated inhalation exposure to 1-bromopropane.
The present study investigated the effects of 1-bromopropane (1BP) on brain neuroactive substances of rats to determine the extent of its toxicity to the central nervous system (CNS). We measured the changes in neurotransmitters (acetylcholine, catecholamine, serotonin and amino acids) and their metabolites or precursors in eight brain regions after inhalation exposure to 1BP at 50 to 1,000 ppm for 8 h per day for 7 d per week for 3 wk. Rats were sacrificed at 2 h (Case 1), or at 19 h (Case 2) after the end of exposure. In Case 1, the level of 5-hydroxyindoleacetic acid (5HIAA) was lowered in some brain regions by 1BP exposure. The decrease of 5HIAA in the frontal cortex was statistically significant at 50 ppm 1BP exposure. In Case 2, gamma-amino butyric acid (GABA) and taurine were decreased in many brain regions of exposed rats, and a significant decrease of taurine in the midbrain occurred at 50 ppm 1BP exposure. In both cases of 2-h and 19-h intervals from the end of exposure to sacrifice, aspartate and glutamine levels were elevated in many brain regions, but the acetylcholine level did not change in any brain region. Three-week repeated exposure to 1BP produced significantly changes in amino acid contents of rat brains, particularly at 1,000 ppm.
Suda
Megumi
M
National Institute of Occupational Safety and Health (JNIOSH),Kawasaki, Japan.
Honma
Takeshi
T
Miyagawa
Muneyuki
M
Wang
Rui-Sheng
RS
D000280:Administration, Inhalation; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D006842:Hydrocarbons, Brominated / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity; D007564:Japan; D008297:Male; D018377:Neurotransmitter Agents / Q000032:analysis*; D051381:Rats; D011916:Rats, Inbred F344
D000596:Amino Acids; D006842:Hydrocarbons, Brominated; D018377:Neurotransmitter Agents; C118559:1-bromopropane