Effect of combining nisin and/or lysozyme with in-package pasteurization for control of Listeria monocytogenes in ready-to-eat turkey bologna during refrigerated storage.
This study investigated the efficacy of in-package pasteurization combined with pre-surface application of nisin and/or lysozyme to reduce and prevent the subsequent recovery and growth of Listeria monocytogenes during refrigerated storage on the surface of low-fat turkey bologna. Sterile bologna samples were treated with solutions of nisin (2 mg/ml=5000 AU/ml), lysozyme (10 mg/ml=80 AU/ml) and a mixture of nisin and lysozyme (2 mg nisin+10mg lysozyme/ml) before in-package pasteurization at 65 degrees C for 32s. In-package pasteurization resulted in an immediate 3.5-4.2 log CFU/cm(2) reduction in L. monocytogenes population for all treatments. All pasteurized treatments also resulted in a significant reduction of L. monocytogenes by 12 weeks compared to un-pasteurized bologna. In-package pasteurization in combination with nisin or nisin-lysozyme treatments was effective in reducing the population below detectable levels by 2-3 weeks of storage. Results from this study could have a significant impact for the industry since a reduction in bacterial population was achieved by a relatively short pasteurization time and antimicrobials reduced populations further during refrigerated storage.
Mangalassary
Sunil
S
Department of Food Science and Human Nutrition, Clemson University, Clemson, SC 29634, USA.
Han
Inyee
I
Rieck
James
J
Acton
James
J
Dawson
Paul
P
D000818:Animals; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D005506:Food Contamination / Q000517:prevention & control; D005519:Food Preservation / Q000379:methods*; D005520:Food Preservatives / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D009113:Muramidase / Q000494:pharmacology*; D009561:Nisin / Q000494:pharmacology*; D011202:Poultry Products / Q000382:microbiology* / Q000592:standards; D012034:Refrigeration; D013997:Time Factors; D014422:Turkeys
D005520:Food Preservatives; D009561:Nisin; D009113:Muramidase