Translocation of chicken heart apocytochrome c and its mutants (C17S, H18D) across mitochondrial membrane.
The dependence of import of chicken heart apocytochrome c on its transformation to holoform by heme attachment was studied. Results showed that there was no difference in the translocation of apocytochrome c across the mitochondrial membrane in the presence or absence of hemin + dithionite. Furthermore, two heme unattached mutants (H18D. C17S) were prepared, which could still be accumulated in mitochondria, but their import velocity was obviously reduced.
Zhu
Y
Y
Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Han
X
X
Yang
F
F