Homogenous amniotic membrane as a biological dressing for oral mucositis in rats: histomorphometric analysis.
OBJECTIVE
Oral mucositis is a frequent and significant side effect of chemotherapy and/or radiation therapy. The purpose of this study was to evaluate the effects of the homogenous amniotic membrane (HAM) as a biological dressing for oral mucositis induced by 5-fluorouracil (5-FU) in the labial fornix region of inferior incisors in rats.


METHODS
Sixty rats were divided into three groups: control (50% acetic acid), 5-FU (50% acetic acid and 5-fluorouracil-induced oral mucositis) and 5-FU+HAM (50% acetic acid and 5-fluorouracil-induced oral mucositis, and treated with HAM). Histological analyses were done 3, 7, 14 and 21 days after applying the acetic acid.


RESULTS
HAM showed not only no signs of rejection as well as an excellent tissue adherence to the ulcerated surface. Histomorphometric analysis indicated a lower quantity of inflammatory cells in the 5-FU+HAM group in all periods when compared to the other groups and there was a statistically significant difference in the 3- and 7-day periods (P<0.001).


CONCLUSIONS
The results of this study suggest that HAM was biocompatible with great adhesive power to the ulcerated surface, accelerated the healing process and exhibited anti-inflammatory activity. Further studies should be done to investigate the potential benefits of this material in clinical trials. HAM could lead to improved results as a biological dressing in treatment and management of oral mucositis in patients submitted to chemotherapy and/or radiation therapy.
Vilela-Goulart
Maria das Graças
Md
Bioscience Center for Special Health Care Needs (Centro de Biociências Aplicado a Pacientes com Necessidades Especiais/CEBAPE-UNESP), São José dos Campos Dental School, São Paulo State University-UNESP, São Paulo, Brazil.
Teixeira
Raquel Teodoro Santos
RT
Rangel
Daniela Coelho
DC
Niccoli-Filho
Walter
W
Gomes
Mônica Fernandes
MF
D019342:Acetic Acid / Q000008:administration & dosage; D000650:Amnion / Q000637:transplantation*; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000009:adverse effects; D001684:Biological Dressings; D005260:Female; D005472:Fluorouracil / Q000009:adverse effects; D008297:Male; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013280:Stomatitis / Q000139:chemically induced / Q000473:pathology* / Q000628:therapy
D000964:Antimetabolites, Antineoplastic; D019342:Acetic Acid; D005472:Fluorouracil