Proteomic identification of the silkworm (Bombyx mori L) prothoracic glands during the fifth instar stage.
Although the ecdysteroid of the silkworm had been studied for decades, the proteome of the prothoracic gland, the primary source of ecdysteroid hormones, has not been studied previously. In the present paper, we utilized a proteomic approach to investigate the fifth instar prothoracic gland during the growth and development of the silkworm, Bombyx mori L. The two-dimensional electrophoresis results showed that the majority of proteins were acidic proteins, especially concentrated in the area of 25-65 kDa, with pI values of between 4 and 7, and the difference was not distinct. When compared with Qiufeng (Japanese strain), the interspecific distinction was larger than the intraspecific distinction, and 19 particular spots, excized from the third, fifth and ninth days of p50 (Chinese strain) and Qiufeng were subjected to MALDI-TOF-MS (matrix-assisted laser-desorption ionization-time-of-flight MS) analysis. We sorted them into seven catagories: energetics and/or metabolism, storage proteins, protection, lipid metabolism, signal transduction, cell function and unknown function proteins. Of these proteins, arginine methyltransferase is discussed as playing an important role in regulating the activation of ecdysteroidogenesis via transcription or translation.
Wu
Xiao-feng
XF
Key Laboratory of Animal Epidemic Etiology and Immunological Prevention of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310029, People's Republic of China.
Li
Xing-hua
XH
Yue
Wan-fu
WF
Roy
Bhaskar
B
Li
Guang-li
GL
Liu
Jian-mei
JM
Zhong
Bo-xiong
BX
Gao
Qi-kang
QK
David
Wan Chi Cheong
WC
Miao
Yun-gen
YG
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000254:growth & development* / Q000378:metabolism; D003335:Corpora Allata / Q000254:growth & development* / Q000378:metabolism; D026461:Ecdysteroids / Q000235:genetics / Q000378:metabolism; D019476:Insect Proteins / Q000032:analysis*; D007814:Larva / Q000254:growth & development; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D047011:Silk / Q000096:biosynthesis
D026461:Ecdysteroids; D019476:Insect Proteins; D020543:Proteome; D047011:Silk