Isolation and visualization of mouse placental hematopoietic stem cells.
This unit describes the isolation of hematopoietic stem cells (HSCs) from the mouse placenta. The placenta was recently identified as an important hematopoietic site that generates HSCs de novo and provides a transitory niche for a large pool of HSCs during midgestation. This protocol includes a dissection technique for murine placenta, the mechanical and enzymatic steps of placental tissue dissociation, and phenotypical identification and isolation of HSCs. It also contains a method for immunohistochemical analysis of placenta tissue sections to visualize developing HSCs in the placenta.
Gekas
Christos
C
University of California Los Angeles, Los Angeles, California, USA.
Rhodes
Katrin E
KE
Mikkola
Hanna K A
HK
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003656:Decidua / Q000166:cytology; D004210:Dissection; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D005629:Frozen Sections; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007150:Immunohistochemistry; D051379:Mice; D010920:Placenta / Q000166:cytology*; D016707:Tissue Fixation; D015017:Yolk Sac / Q000166:cytology