Reactant stationary approximation in enzyme kinetics.
In the application of the quasi-steady-state approximation, it is generally assumed that there is an initial transient during which the substrate concentration remains approximately constant while the complex concentration builds up. In this paper, we address the assumption that the substrate concentration does not change significantly during this initial transient and name it the reactant stationary approximation. For the single enzyme, single substrate reaction, the reactant stationary approximation is generally considered a sufficient condition to apply the quasi-steady-state approximation. Studying the dynamic behavior of this reaction with endogenous substrate, we show that the quasi-steady-state approximation and reactant stationary approximation are two separate approximations. We discuss the consequence of this result for the determination of reaction parameters in enzyme catalyzed reactions.
Hanson
Sonya M
SM
Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089-0484, USA.
Schnell
Santiago
S
D003198:Computer Simulation; D004735:Energy Transfer; D004798:Enzymes / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical
D004798:Enzymes