Phylogenetic characterization of two novel commensal bacteria involved with innate immune homeostasis in Drosophila melanogaster.
During a previous study on the molecular interaction between commensal bacteria and host gut immunity, two novel bacterial strains, A911(T) and G707(T), were isolated from the gut of Drosophila melanogaster. In this study, these strains were characterized in a polyphasic taxonomic study using phenotypic, genetic, and chemotaxonomic analyses. We show that the strains represent novel species in the family Acetobacteraceae. Strain G707(T), a highly pathogenic organism, represents a new species in the genus Gluconobacter, "Gluconobacter morbifer" sp. nov. (type strain G707 = KCTC 22116(T) = JCM 15512(T)). Strain A911(T), dominantly present in the normal Drosphila gut community, represents a novel genus and species, designated "Commensalibacter intestini" gen. nov., sp. nov. (type strain A911 = KCTC 22117(T) = JCM 15511(T)).
Roh
Seong Woon
SW
University of Science & Technology, 52 Eoeun-dong, Daejeon 305-333, Republic of Korea. baejw@kribb.re.kr.
Nam
Young-Do
YD
Chang
Ho-Won
HW
Kim
Kyoung-Ho
KH
Kim
Min-Soo
MS
Ryu
Ji-Hwan
JH
Kim
Sung-Hee
SH
Lee
Won-Jae
WJ
Bae
Jin-Woo
JW
D016947:Acetobacteraceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004331:Drosophila melanogaster / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S