title: High-resolution analysis of genomic profiles of hepatocellular carcinoma cells with differential osteopontin expression.
abstract: Overexpression of osteopontin (OPN) could contribute to tumorigenesis and metastasis in hepatocellular carcinoma (HCC). Previous studies have shown that OPN-positive cancer cells are often localized in the periphery of cancer nodules adjacent to stromal cells. To identify the difference in intratumor genomic aberration pattern between OPN-positive and OPN-negative HCC cells, we adopted microarray-based comparative genomic hybridization (array-CGH) to achieve high-resolution analysis of genome-wide aberrations. Our present study indicates that, compared to OPN-negative cancer cells, OPN-positive cancer cells show much more amplification of chromosomal regions, including 4q13.1-q13.3, 4q21.23-q22.1 and 13q32.1-q32.3. Some candidate tumor related genes, such as SMR3B, MUC7, EPHA5, SPP1 and CLDN10, were detected with over 1.5-fold amplification. Together, the intratumor genomic heterogeneity may suggest that OPN-positive cancer cells play a more important role in the development of HCC malignancy than their OPN-negative counterparts.
authors: Tianjin Medical University Cancer Institute and Hospital; Tianjin Medical University; Tianjin, China.
authors: Bingsheng
authors: B
authors: Sun
authors: Jincai
authors: J
authors: Wu
authors: Ti
authors: T
authors: Zhang
authors: Changli
authors: C
authors: Wang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D005784:Gene Amplification; D020869:Gene Expression Profiling; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D053495:Osteopontin / Q000235:genetics*
chemical_list: D053495:Osteopontin