Expression of caveolin-3 immunoreactivities in the developing sciatic nerve of the rat.
Caveolae are formed by the caveolin (CAV) family of proteins, CAV-1, -2, and -3. CAV-1 and -2 are co expressed in many cell types, whereas CAV-3 is muscle-specific and mutation of the CAV-3 gene causes muscular dystrophy. CAV-3 has also been detected in brain astroglial cells and in peripheral nerves along with CAV-1. Therefore, we sought to determine whether CAV-3 protein is expressed in developing peripheral nerves by using immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). We found that CAV-3 immunoreactivities (IRs) were Localized in the myelin sheath during peripheral nerve development. CAV-3 IRs were intense during the early postnatal stage, but decreased as the peripheral nerves matured at postnatal weeks 3-5. CAV-3 mRNA expression was also markedly decreased during postnatal development. Because the expression pattern of CAV-3 IRs was opposite that of CAV-1 IRs. CAV-1 and -3 might be involved in different phases of peripheral nerve myelination and play complementary roles in myelin maturation and peripheral nerve development.
Lee
Ho J
HJ
Department of Rehabilitation Medicine, Dongguk University College of Medicine, Gyeongju, Korea.
Park
Choong H
CH
Lee
Seong J
SJ
Park
Jin W
JW
Choi
Jong H
JH
Ryu
Gi H
GH
Kwon
Bum S
BS
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D051242:Caveolin 1 / Q000378:metabolism; D051244:Caveolin 3 / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007211:Indoles; D004676:Myelin Basic Protein / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000254:growth & development* / Q000378:metabolism*
D051242:Caveolin 1; D051244:Caveolin 3; D007211:Indoles; D004676:Myelin Basic Protein; D016900:Neurofilament Proteins; D012333:RNA, Messenger; C061195:neurofilament protein M; C007293:DAPI