In vitro transient expression system of latex C-serum was used for analysis of hevein promoter in response to abscisic acid in Hevea brasiliensis.
Hevein has been found to be an essential element in coagulation of rubber particles in latex of rubber trees. In a previous study, we cloned a 1,241-bp fragment of a 5' upstream region of the hevein gene by genome walking. This fragment was analyzed by a 5' end nested deletion method in the present study, fused with a uidA (gus) gene to produce a series of tested constructs, which were transferred into C-serum of latex and the Gus activities were detected. Results showed that the fragment from -749 to -292 was sufficient for expression of gus gene in latex, and the fragment from -292 to -168 was crucial in response to abscisic acid inducement. In a transient transgenic test of rubber leaf with particle bombardment, construct Hev749 conferred gus-specific expression in veins, in which the latex tubes mainly distributed. This implies that the fragment from -749 to -292 was laticiferous-specific.
Fei
Xiao-Wen
XW
Institute of Tropical Bioscience and Biotechnology, Chinese Agricultural Academy for Tropical Crops, Haikou 571101, China.
Deng
Xiao-Dong
XD
D000040:Abscisic Acid / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D020029:Base Pairing / Q000187:drug effects; D002498:Centrifugation; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D028482:Hevea / Q000187:drug effects* / Q000235:genetics; D007840:Latex / Q000378:metabolism*; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D037121:Plant Lectins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017384:Sequence Deletion
D023181:Antimicrobial Cationic Peptides; D007840:Latex; D037121:Plant Lectins; C045875:hevein; D000040:Abscisic Acid; D005966:Glucuronidase