Exon structure requirements for yeast tRNA ligase.
Different nucleotides were introduced into nucleotides 32, 37 and 38 of yeast tRNA(Phe) precursors via oligonucleotide directed mutations. Pre-tRNAs were prepared using T7-transcription in vitro and spliced with the purified yeast tRNA endonuclease and tRNA ligase. It is demonstrated that tRNA ligase activities will be inhibited by the 5'-double-stranded end of 3'-halves.
Liu
J
J
Shanghai Research Center of Life Sciences, Chinese Academy of Sciences, China.
Jin
Y
Y
Wang
D
D