[Effect of Xinnao Shutong capsule on apoptosis after cerebral ischemia and reperfusion in rats].
OBJECTIVE
To study apoptosis-regulating cytokines and apoptosis on focal cerebral ischemia and reperfusion in rats treated with Xinnao Shutong capsule.


METHODS
Rat models of focal cerebral ischemia and reperfusion were established by thread ligation in middle cerebral artery occlusions (MCAO). After 24 hours, the brains were removed to detect changes of protein expression of Bax, Bcl-2, Fas, Fas-L and caspase-3 by immuno-hisochemistry, and apoptosis of cortical neurons by TUNEL RESULT: Compared to control, brain cortex have decreasing the protein expression of Bcl-2 and the ratio of Bcl-2/Bax, increasing the protein expression of Bax, Fas, Fas-L and caspase-3 of ischemia and reperfusion models group (P < 0.01). Xinnao Shutong capsule group could increase the protein expression of Bcl-2 and the ratio of Bcl-2/Bax, and obviously decrease the protein expression of Bax, Fas, Fas-L and caspase-3, then reduce the number of apoptotic cells of cortex (P < 0.01).


CONCLUSIONS
Xinnao Shutong capsule protect injured rat brain tissue, may be related to decrease neuronal apoptosis and adjusted protein expression of apoptosis-regulating cytokines.
Zhang
Yun-Ling
YL
Dong Fang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China. yunling-zhang2004@yahoo.com.cn
Liu
Xue-Mei
XM
Liu
Hong-Sheng
HS
Lou
Jin-Li
JL
Zheng
Hong
H
Huang
Qi-Fu
QF
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000601:surgery; D002214:Capsules / Q000008:administration & dosage; D002544:Cerebral Infarction / Q000188:drug therapy* / Q000378:metabolism / Q000601:surgery; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion
D051017:Apoptosis Regulatory Proteins; D002214:Capsules; D004365:Drugs, Chinese Herbal; C528303:Xinnao Shutong