Synthesis of O-phosphopeptides on solid phase.
Most mammalian proteins are transiently phosphorylated on seryl, threonyl, or tyrosyl positions by kinases and dephosphorylated by phosphatases in response to specific intra- or extracellular signals. Many diseases, such as cancer, are caused by altered kinase and phosphatase activities or changed expression levels of either of these enzymes. Thus phosphopeptides are important and universal tools to study disease-specific changes, such as protein-protein/DNA interactions or hyperactive kinases, using phosphopeptides or the corresponding mimics. Here we describe two generally applicable techniques to synthesize multiply phosphorylated peptides as basic tools for drug development. The phosphopeptides were purified to homogeneity and characterized by mass spectrometry.
Singer
David
D
Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, Faculty of Chemistry and Mineralogy, University of Leipzig, Leipzig, Germany.
Hoffmann
Ralf
R
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005449:Fluorenes / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism
D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010748:Phosphopeptides; D010744:Phosphoric Monoester Hydrolases