[Effect of highway transportation on the quality of red blood cells].
This study was purposed to investigate the effect of highway transportation on the quality of blood components so as to provide experimental basis to meet the needs of military operations. The transport condition was simulated by random vibration test. The red blood cells, leukocyte-reduced red blood cells, washed red blood cells were randomly vibrated (C Road) for 4 hours. Then, these blood components were stored in refrigerator for 15 days (4 degrees C). Six milliliters of blood were collected before vibration, after vibration, and at day 15 days of storage after vibration, respectively. The suspension was isolated. The free hemoglobin (FHb), routine hematological parameters, and biochemical indexes were determined. The results showed that FHb, lactate dehydrogenase (LDH), K(+) of red blood cells and leukocyte-reduced red blood cells did not significantly change after vibration and storage. However, FHb, LDH and K(+) of washed red blood cells increased significantly after simulated transportation (p < 0.05). The levels of these parameters at day 15 of storage after vibration were also significantly higher than those after vibration (p < 0.01). The changes of other hematological parameters were not significant in three blood components after vibration (C Road) and storage for 15 day. In conclusion, red blood cells and leukocyte-reduced red blood cells were qualified for clinic transfusion even after transportation within 4 hours for 15 day storage later, if they were kept in proper blood container and protected from damping. However, the washed red blood cells could not be used for clinic after similar transport in the military operations.
Yao
Gen-Hong
GH
Department of Blood Transfusion, Nanjing General Hospital of Nanjing Military Area, PLA, Nanjing 210002, Jiangsu Province, China.
Luan
Jian-Feng
JF
Ye
Dong
D
Zhu
Pei-Yuan
PY
Yan
Jing-Mei
JM
Hong
Ping
P
Hu
Xin-Yong
XY
Yi
Xue-Ming
XM
D001793:Blood Preservation; D015925:Cryopreservation; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D014186:Transportation; D014732:Vibration
D007770:L-Lactate Dehydrogenase