Effect of a calcium hydroxide-based paste associated to chlorhexidine on RAW 264.7 macrophage cell line culture.
OBJECTIVE
The objective of this study was to evaluate the effect of a calcium hydroxide Ca(OH)(2)-based paste (Calen) associated or not to 0.4% chlorhexidine digluconate (CHX) on RAW 264.7 macrophage cell line culture.


METHODS
The cell viability (MTT assay), immunostimulating properties (NO dosage), and anti-inflammatory properties (NO, TNF-alpha, and IL-1alpha dosage) were evaluated after cell exposure to the materials. Data were analyzed statistically by Kruskal-Wallis test at 5% significance level.


RESULTS
There was low immunostimulating activity of the Calen paste associated or not to 0.4% CHX in the different materials' concentrations evaluated (P > .05). Anti-inflammatory activity with inhibition of NO and cytokine (TNF-alpha and IL1-alpha) release was observed only with Ca(OH)(2) + CHX at the highest concentration (25 microg/mL).


CONCLUSIONS
As the Calen paste associated to 0.4% CHX did not alter cell viability or the immunostimulating and anti-inflammatory properties, the addition of CHX brought no benefits to the Ca(OH)(2)-based paste with regard to the tested parameters.
da Silva
Raquel Assed Bezerra
RA
Department of Pediatric Clinics, Preventive and Community Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Leonardo
Mário Roberto
MR
da Silva
Léa Assed Bezerra
LA
Faccioli
Lúcia Helena
LH
de Medeiros
Alexandra Ivo
AI
D000891:Anti-Infective Agents, Local / Q000494:pharmacology / Q000633:toxicity*; D002126:Calcium Hydroxide / Q000494:pharmacology / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002710:Chlorhexidine / Q000494:pharmacology / Q000633:toxicity*; D004338:Drug Combinations; D004357:Drug Synergism; D006801:Humans; D053582:Interleukin-1alpha / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects*; D009569:Nitric Oxide / Q000096:biosynthesis; D012387:Root Canal Filling Materials / Q000494:pharmacology / Q000633:toxicity; D012388:Root Canal Irrigants / Q000494:pharmacology / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D053582:Interleukin-1alpha; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D014409:Tumor Necrosis Factor-alpha; C420643:calen; D009569:Nitric Oxide; D002126:Calcium Hydroxide; D002710:Chlorhexidine