title: Multiplexed glycoproteomic analysis of glycosylation disorders by sequential yolk immunoglobulins immunoseparation and MALDI-TOF MS.
abstract: This study applied yolk immunoglobulins immunoaffinity separation and MALDI-TOF MS for clinical proteomics of congenital disorders of glycosylation (CDG) and secondary glycosylation disorders [galactosemia and hereditary fructose intolerance (HFI)]. Serum transferrin (Tf) and alpha1-antitrypsin (AAT) that are markers for CDG, were purified sequentially to obtain high-quality MALDI mass spectra to differentiate single glycoforms of the native intact glycoproteins. The procedure was found feasible for the investigation of protein macroheterogeneity due to glycosylation site underoccupancy then ensuing the characterization of patients with CDG group I (N-glycan assembly disorders). Following PNGase F digestion of the purified glycoprotein, the characterization of protein microheterogeneity by N-glycan MS analysis was performed in a patient with CDG group II (processing disorders). CDG-Ia patients showed a typical profile of underglycosylation where the fully glycosylated glycoforms are always the most abundant present in plasma with lesser amounts of partially and unglycosylated glycoforms in this order. Galactosemia and HFI are potentially fatal diseases, which benefit from early diagnosis and prompt therapeutic intervention. In symptomatic patients with galactosemia and in those with HFI, MALDI MS of Tf and AAT depicts a hypoglycosylation profile with a significant increase of underglycosylated glycoforms that reverses by dietary treatment, representing a clue for diagnosis and treatment monitoring.
authors: Istituto di Chimica e Tecnologia dei Polimeri, CNR, Catania, Italy.
authors: Luisa
authors: L
authors: Sturiale
authors: Rita
authors: R
authors: Barone
authors: Angelo
authors: A
authors: Palmigiano
authors: Célestin Nsibu
authors: CN
authors: Ndosimao
authors: Paz
authors: P
authors: Briones
authors: Maciej
authors: M
authors: Adamowicz
authors: Jaak
authors: J
authors: Jaeken
authors: Domenico
authors: D
authors: Garozzo
mesh_terms: D000293:Adolescent; D000328:Adult; D002239:Carbohydrate Metabolism, Inborn Errors / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004527:Egg Proteins / Q000378:metabolism*; D005260:Female; D005693:Galactosemias / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D008297:Male; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D055815:Young Adult
chemical_list: D004527:Egg Proteins; D006023:Glycoproteins; D007136:Immunoglobulins