[Separation and purification of flavonoids from Smilax glabra by macroporous adsorption resin].
OBJECTIVE
To investigate the process of separating and purifying flavonoids from Smilax glabra.


METHODS
With the yield of flavonoids as index, the optimum process of separating and purifying flavonoids from S. glabra Roxb was screened by static and dynamic adsorption tests.


RESULTS
The static saturated adsorption capacity of D101 macroporous resin to flavonoids of S. glabra was 45.6 mg x g(-1) (dry resin). The optimum conditions of dynamic adsorption and elution were as that the pH, the concentration, the adsorption velocity of the extracting solution, and the adsorption capacity were 6.00 +/- 0.20, 4.2 mg x mL(-1), 2 mL x min(-1) and 15 mL, respectively. The adsorbed resin column was washed by 100 mL 60% ethanol with pH value of 8.00 +/- 0.20 at the eluting velocity of 3 mL x min(-1) after washed by 100 mL distilled water.


CONCLUSIONS
The flavonoids of S. glabra was able to be easily separated and purified by D101 macroporous resin under the optimum conditions above, and the recovery rate was higher than 90%. The content of obtained flavonoids reached 62.6%, which was 2 times of the content before purification.
Huang
Shao-Wei
SW
Hubei Key Lab of Novel Reactor & Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, China.
Chi
Ru-An
RA
Zhang
Yue-Fei
YF
Wu
Yuan-Xin
YX
Ju
Xia-Lian
XL
Guo
Jia
J
Huang
Qi-Mao
QM
D000327:Adsorption; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry*; D032281:Smilax / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012117:Resins, Synthetic