Simultaneous isolation of six fluoroquinolones in serum samples by selective molecularly imprinted matrix solid-phase dispersion.
A simple and sensitive method for the simultaneous determination of six fluoroquinolones from serum samples was developed by selective molecularly imprinted matrix solid-phase dispersion (MI-MSPD) coupled with chromatographic separation. By using ethylene glycol dimethacrylate as crosslinker and reformative methanol-water system as reaction medium, the improved water-compatible imprinted polymers were synthesized which show higher affinity to template and its analogues in aqueous environment. The molecularly imprinted polymers (MIPs) were applied as the selective dispersant of matrix solid-phase dispersion (MSPD) could selectively extracted the six fluoroquinolones from serum, while interferences originated from serum matrices were eliminated simultaneously. Good linearity was obtained in a range of 0.05-100 microg mL(-1) with the correlation coefficients >0.999. The average recoveries of the six fluoroquinolones at four different spiked levels (0.25-10 microg mL(-1)) were ranged from 72.2% to 114.1% with the relative standard deviations less than 6.6%. This method is simple and sensitive, and can be used as an alternative tool to the existing HPLC methods for analyzing the residues of fluoroquinolones in biological samples.
Sun
Hanwen
H
College of Chemical and Environmental Sciences, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding, 071002, China.
Qiao
Fengxia
F
Liu
Guangyu
G
Liang
Shuxuan
S
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D024841:Fluoroquinolones / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D016014:Linear Models; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
D024841:Fluoroquinolones; D011108:Polymers