title: Nicotine inhibits mineralization of human dental pulp cells.
abstract: Nicotine is a major component of tobacco smoke, and signals via nicotinic acetylcholine receptors (nAChR). However, little is known about the effects of nicotine on human dental pulp cells (HDPCs). In this study, we assessed the effects of nicotine on mineralization in HDPCs. We confirmed messenger RNA expression of nAChR subunits and examined the effects of nicotine on expression of extracellular matrices (ECMs), alkaline phosphatase (ALP) activity, and mineralized nodule formation by HDPCs. Gene expression of nAChR subunits alpha1, alpha2, alpha 4, alpha 5, alpha 6, alpha 7, beta1, beta2, and beta 4 was detected in HDPCs. Interestingly, the messenger RNA expression of dentin matrix acidic phosphoprotein-1, bone sialoprotein, and ALP activity were significantly reduced in nicotine-treated HDPC. In addition, mineralized nodule formation, which was examined by alizarin red staining, was also inhibited in HDPCs by the same treatment. These results indicate that nicotine suppresses the cytodifferentiation and mineralization of HDPCs, possibly via nAChR.
authors: Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors: Manabu
authors: M
authors: Yanagita
authors: Yoichiro
authors: Y
authors: Kashiwagi
authors: Ryohei
authors: R
authors: Kobayashi
authors: Miki
authors: M
authors: Tomoeda
authors: Yoshio
authors: Y
authors: Shimabukuro
authors: Shinya
authors: S
authors: Murakami
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D006801:Humans; D058574:Integrin-Binding Sialoprotein; D009538:Nicotine / Q000633:toxicity*; D018722:Nicotinic Agonists / Q000633:toxicity*; D010750:Phosphoproteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D011978:Receptors, Nicotinic / Q000096:biosynthesis; D012795:Sialoglycoproteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D014074:Tooth Calcification / Q000187:drug effects*
chemical_list: C513309:DMP1 protein, human; D016326:Extracellular Matrix Proteins; C546871:IBSP protein, human; D058574:Integrin-Binding Sialoprotein; D018722:Nicotinic Agonists; D010750:Phosphoproteins; D011978:Receptors, Nicotinic; D012795:Sialoglycoproteins; D009538:Nicotine