Chitin synthases are required for survival, fecundity and egg hatch in the red flour beetle, Tribolium castaneum.
The synthesis of chitin, the beta-1,4-linked polymer of N-acetylglucosamine, is catalyzed by chitin synthase (CHS). Chitin is essential for the structural integrity of the exoskeletal cuticle and midgut peritrophic membrane (PM) of insects. To study the functions of the two chitin synthase genes, TcCHS-A and TcCHS-B, during embryonic and adult development in the red flour beetle, Tribolium castaneum, RNA interference (RNAi) experiments were carried out. When dsRNA for TcCHS-A was injected into male or female pharate adults, all insects died 5-7 d after the adult molt, and the females failed to oviposit prior to death. When dsTcCHS-A was injected into young adults 1-2 d post-eclosion, a similar lethal phenotype was obtained after 5 d and no oviposition occurred. When dsTcCHS-A injections were delayed until after adult maturation (7-10 d post-eclosion), the treated females did oviposit and the resulting embryos appeared to develop normally. However, the chitin content of the eggs was dramatically reduced, the embryos became twisted and enlarged, and the eggs did not hatch. Adults treated with dsRNA for TcCHS-B exhibited little or no chitin in their PM and died about 2 wk after injection. None of the TcCHS-B-treated females oviposited, which was probably a secondary effect caused by starvation. These results extend our previous findings that CHS genes are required for all types of molt. The present study also demonstrates that these genes have additional roles in embryonic and adult development.
Arakane
Yasuyuki
Y
Department of Biochemistry, Kansas State University, Manhattan, KS 66506-3702, USA. subaru@ksu.edu
Specht
Charles A
CA
Kramer
Karl J
KJ
Muthukrishnan
Subbaratnam
S
Beeman
Richard W
RW
D000818:Animals; D002687:Chitin Synthase / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008297:Male; D052287:Oviparity; D010063:Ovum / Q000502:physiology*; D010641:Phenotype; D034622:RNA Interference; D014231:Tribolium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D002687:Chitin Synthase