Identification of a novel DNA-binding protein to osmotin promoter.
One novel osmotin promoter, binding-protein (OPBP1) gene, was isolated from salt-adapted tobacco suspension cells using yeast one-hybrid system. The OPBP1 interacted specifically in vivo with FA, a DNA sequence from the 5 upstream region of osmotin gene, which was essential for osmotin responsiveness. The deduced amino acid sequence of OPBP1 contained a conserved motif of a new gene family, AP2 family. This protein did not contain the typical motif found in the most known DNA-binding proteins and transcription factors.
Xu
P
P
Biotechnology Institute, Zhejiang Agricultural University, Hangzhou, China.
Ling
J
J
Li
D
D
Hasegawa
P M
PM
Bressan
R A
RA