Electroporation-based DNA transfer enhances gene expression and immune responses to DNA vaccines in cattle.
Despite the potential of DNA vaccines to induce strong, balanced immune responses in small experimental species, the immune responses to DNA immunization in larger species have generally been moderate and inconsistent. In this study, the TriGridtrade mark Delivery System (TDS), an electroporation-based DNA delivery platform, was evaluated for administration of DNA vaccines to calves. When compared to conventional intramuscular delivery, TDS-based delivery markedly and consistently enhanced gene expression from a plasmid encoding a reporter gene, secreted alkaline phosphatase, and improved cell-mediated and humoral immune responses to a plasmid encoding a model antigen, hepatitis B surface antigen. Importantly, the TDS-based procedure was well tolerated by the calves, which did not need to be anesthetized or sedated. These results suggest that the TDS is a useful delivery method for DNA vaccines in cattle.
van Drunen Littel-van den Hurk
S
S
Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada. sylvia.vandenHurk@usask.ca
Luxembourg
A
A
Ellefsen
B
B
Wilson
D
D
Ubach
A
A
Hannaman
D
D
van den Hurk
J V
JV
D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D002417:Cattle; D004247:DNA / Q000008:administration & dosage* / Q000276:immunology*; D016503:Drug Delivery Systems; D018274:Electroporation; D004797:Enzyme-Linked Immunosorbent Assay; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology*; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology*
D006514:Hepatitis B Surface Antigens; D019444:Vaccines, DNA; D007371:Interferon-gamma; D004247:DNA