Microtubule alignment and manipulation using AC electrokinetics.
The kinesin-microtubule system plays an important role in intracellular transport and is a model system for integrating biomotor-driven transport into microengineered devices. AC electrokinetics provides a novel tool for manipulating and organizing microtubules in solution, enabling new experimental geometries for investigating and controlling the interactions of microtubules and microtubule motors in vitro. By fabricating microelectrodes on glass substrates and generating AC electric fields across solutions of microtubules in low-ionic-strength buffers, bundles of microtubules are collected and aligned and the electrical properties of microtubules in solution are measured. The AC electric fields result in electro-osmotic flow, electrothermal flow, and dielectrophoresis of microtubules, which can be controlled by varying the solution conductivity, AC frequency, and electrode geometry. By mapping the solution conductivity and AC frequency over which positive dielectrophoresis occurs, the apparent conductivity of taxol-stabilized bovine-brain microtubules in PIPES buffer is measured to be 250 mS m(-1). By maximizing dielectrophoretic forces and minimizing electro-osmotic and electrothermal flow, microtubules are assembled into opposed asters. These experiments demonstrate that AC electrokinetics provides a powerful new tool for kinesin-driven transport applications and for investigating the role of microtubule motors in development and maintenance of the mitotic spindle.
Uppalapati
Maruti
M
Department of Bioengineering 229 Hallowell Bldg. Penn State University University Park, PA 16802, USA.
Huang
Ying-Ming
YM
Jackson
Thomas N
TN
Hancock
William O
WO
D000818:Animals; D002021:Buffers; D002417:Cattle; D003198:Computer Simulation; D004566:Electrodes; D004583:Electrons; D016547:Kinesins / Q000737:chemistry; D007700:Kinetics; D008870:Microtubules / Q000737:chemistry*; D009994:Osmolar Concentration; D013696:Temperature; D014867:Water / Q000737:chemistry
D002021:Buffers; D014867:Water; D016547:Kinesins