Simultaneous measurement of brain tissue oxygen partial pressure, temperature, and global oxygen consumption during hibernation, arousal, and euthermy in non-sedated and non-anesthetized Arctic ground squirrels.
This study reports an online temperature correction method for determining tissue oxygen partial pressure P(tO2) in the striatum and a novel simultaneous measurement of brain P(tO2) and temperature (T(brain)) in conjunction with global oxygen consumption V(O2) in non-sedated and non-anesthetized freely moving Arctic ground squirrels (AGS, Spermophilus parryii). This method fills an important research gap-the lack of a suitable method for physiologic studies of tissue P(O2) in hibernating or other cool-blooded species. P(tO2) in AGS brain during euthermy (21.22+/-2.06 mmHg) is significantly higher (P=0.016) than during hibernation (13.21+/-0.46 mmHg) suggests brain oxygenation in the striatum is normoxic during euthermy and hypoxic during hibernation. These results in P(tO2) are different from blood oxygen partial pressure P(aO2) in AGS, which are significantly lower during euthermy than during hibernation and are actually hypoxic during euthermy and normoxic during hibernation in our previous study. This intriguing difference between the P(O2) of brain tissue and blood during these two physiological states suggests that regional mechanisms in the brain play a role in maintaining tissue oxygenation and protect against hypoxia during hibernation.
Ma
Yilong
Y
Alaska Basic Neuroscience Program, Institute of Arctic Biology, University of Alaska, Fairbanks, AK 99775-7000, United States. ffym1@uaf.edu
Wu
Shufen
S
D000758:Anesthesia; D000818:Animals; D001143:Arousal / Q000502:physiology*; D001831:Body Temperature; D001921:Brain / Q000378:metabolism*; D002153:Calorimetry, Indirect / Q000379:methods; D003243:Consciousness; D004567:Electrodes, Implanted; D005260:Female; D006605:Hibernation / Q000502:physiology*; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology*; D010313:Partial Pressure; D012589:Sciuridae