title: Transient expression of hepatitis B virus surface antigen (HBsAg) and human erythropoietin (hEPO) genes in milk after direct introduction into ewe.
abstract: The ability to deliver nonviral DNA directly into animals would provide major practical and economic benefits. To pursue this potential, as a preliminary approach, the expression in mammary epithelial cells with nonviral DNA ending HBsAg and hEPO was investigated by in vivo delivery directly into mammary gland Either the bovine asl-casein-HBsAg or the WAE-hEPO gene construct was introduced into mammary glands of dm by mammary artery injection. A single injection of 10(11) copies of DNA construct was implemented for a doe. Transient expression of the constructs in milk collected from milk letdown by induction up to 19 d after injection was measured with an hEPO ELISA kit from Boehringer Mannheim (Cat. No. 1 693 417) for rhEPO, and a HBsAg-ELISA kit (Abbott Lab. USA) for rHBsAg. The treated animals showed mean concentration of recombinant molecules at 16 microg/L for rHBsAg, and 2 mg/L for rhEPO in milk.
authors: Institute of Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors: W
authors: W
authors: Lao
authors: S
authors: S
authors: Xu
authors: Y
authors: Y
authors: Cheng
authors: X
authors: X
authors: Zhang
authors: J
authors: J
authors: Zhang
authors: G
authors: G
authors: Cheng
authors: X
authors: X
authors: Wang