Expression and putative function of lymphocyte endothelial epithelial-cell adhesion molecule in human testis.
The testis is an immunologically privileged site where germ cell antigens are protected from autoimmune attack and foreign tissue grafts may survive for extended periods. However, the testicular environment does not preclude inflammatory reactions and tissue-specific recruitment of T lymphocytes appears to be a crucial component of the inflammation cascade. Here, we demonstrate expression of lymphocyte endothelial epithelial-cell adhesion molecule (LEEP-CAM), a putative receptor mediating lymphocyte adhesion to endothelia and some epithelia, in human testis. In all specimens examined, expression of LEEP-CAM could be observed on endothelial cells of testicular blood vessels, including those within the lamina propria of seminiferous tubules. Sections of histologically normal testis showed strong LEEP-CAM expression within the seminiferous epithelium localised to Sertoli cells, whereas immunoreactivity was almost absent in tubules with severely impaired spermatogenesis. In a modified Stamper-Woodruff adhesion assay, binding of activated lymphocytes to normal testicular tissue was reduced by 61% after incubation with anti-LEEP-CAM mAb as compared with controls (P < 0.00001). In conclusion, intratubular LEEP-CAM expression is correlated with normal spermatogenesis and Sertoli cell function. In this context, it may contribute to adhesive cell-cell interactions. Moreover, the constitutive expression in human testis could play a role for localisation of T cells during testicular inflammation.
Wienrich
B G
BG
Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, and Department of Dermatology, University of Würzburg, Würzburg, Germany.
Schuppe
H-C
HC
Schön
M P
MP
D000328:Adult; D001706:Biopsy; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008297:Male; D012671:Seminiferous Tubules / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology; D013737:Testis / Q000098:blood supply / Q000166:cytology* / Q000378:metabolism*
D015815:Cell Adhesion Molecules; C120427:LEEP-CAM protein, human