Apoptosis in the trabecular meshwork of glaucomatous patients.
We established and validated an in toto method to perform TdT-mediated dUTP nick end labeling to study apoptosis in human trabecular meshwork tissue obtained during trabeculectomy in glaucoma patients. In specimens from patients with primary open-angle glaucoma and primary angle-closure glaucoma, we detected a tendency for more apoptotic cells to accumulate in patients with primary open-angle glaucoma. The utility of this method to study apoptosis in the trabecular meshwork is discussed, as well as its application as a tool in biologic samples.
Baleriola
Jimena
J
3D Lab (Development, Differentiation, & Degeneration), Department of Cellular and Molecular Physiopathology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
García-Feijoo
Julián
J
Martínez-de-la-Casa
José M
JM
Fernández-Cruz
Arturo
A
de la Rosa
Enrique J
EJ
Fernández-Durango
Raquel
R
D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D015812:Glaucoma, Angle-Closure / Q000473:pathology*; D005902:Glaucoma, Open-Angle / Q000473:pathology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008875:Middle Aged; D014129:Trabecular Meshwork / Q000473:pathology*