title: Bone marrow lacking integrin expression facilitates an enhanced susceptibility to EAE in the xenogeneic bone marrow chimeras.
abstract: Xenotransplantation of rat bone marrow cells (BMC) into immunodeficient (SCID) mice generates chimeric mice susceptible to paralytic autoimmune CNS inflammation. Herein, we identified a disease relevant subset of transplantable BMC lacking expression of CD11b/c and CD49d. Moreover, disease susceptibility was enhanced in the presence of non-myelin specific T-cells. Only the CD11b/c negative population of BM retained the capability to populate the blood, spleen and spinal cord of recipients and matured after transplant to express CD11b/c. These results indicate non-myelin T cells in combination with integrin negative BM represent pre-pathogenic determinants of an enhanced disease susceptibility to myelin reactive T cells.
authors: V.A. Medical Center, R and D-23, 3710 SW Veterans Hospital Rd., Portland, OR 97239, United States.
authors: Kristine M
authors: KM
authors: Robinson
authors: Sheila
authors: S
authors: Markwardt
authors: Nick
authors: N
authors: Kitzerow
authors: Nicole
authors: N
authors: Moes
authors: Mary
authors: M
authors: Estrada
authors: Richard E
authors: RE
authors: Jones
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000379:methods*; D039481:CD11b Antigen / Q000276:immunology / Q000378:metabolism; D039521:CD11c Antigen / Q000276:immunology / Q000378:metabolism; D017690:Cell Transplantation / Q000379:methods; D002678:Chimera; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000601:surgery*; D005260:Female; D016130:Immunophenotyping / Q000379:methods; D039423:Integrin alpha1 / Q000378:metabolism; D016023:Integrins / Q000172:deficiency*; D051379:Mice; D016513:Mice, SCID; D051381:Rats; D011917:Rats, Inbred Lew; D012720:Severity of Illness Index; D014183:Transplantation, Heterologous / Q000379:methods*
chemical_list: D039481:CD11b Antigen; D039521:CD11c Antigen; D039423:Integrin alpha1; D016023:Integrins