Rescue of renal hypoplasia and cystic dysplasia in Bcl-2 -/- mice expressing Bcl-2 in ureteric bud derived epithelia.
Bcl-2 is the founding member of a family of proteins that influence apoptosis. Loss of bcl-2 results in renal hypoplasia/cystic dysplasia at birth. Here, we examined whether re-expression of bcl-2 throughout the ureteric bud and its derived epithelia would restore a normal renal phenotype in bcl-2 -/- mice. Re-expression of bcl-2 in the ureteric bud/collecting duct of bcl-2 -/- mice increased nephron numbers, diminished glomerular hypertrophy, and increased nephrogenic zone size. Unlike bcl-2 -/- mice which have gross renal cyst formation, few renal cysts were present in mice re-expressing bcl-2. We have previously shown increased apoptosis and proliferation, as well as aberrant protein tyrosine phosphatase 1B expression, accompanied cystic changes in bcl-2 -/- mice. These changes were not observed when bcl-2 was re-expressed in the ureteric bud/collecting duct system. Thus, expression of bcl-2 in the ureteric bud/collecting duct resulted in increased nephron numbers partially rescuing renal hypoplasia/cystic dysplasia in bcl-2 -/- mice.
Kondo
Shuji
S
Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53792-4108, USA.
Oakes
Mason G
MG
Sorenson
Christine M
CM
D000818:Animals; D015153:Blotting, Western; D004848:Epithelium / Q000196:embryology / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D007668:Kidney / Q000002:abnormalities / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009399:Nephrons / Q000002:abnormalities / Q000378:metabolism; D011247:Pregnancy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014513:Ureter / Q000166:cytology / Q000196:embryology / Q000378:metabolism*
D019253:Proto-Oncogene Proteins c-bcl-2