Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication.
This study aimed to discover potential biomarkers for dioxynivalenol (DON) intoxication. B6C3F1 male mice were orally exposed to 0.83, 2.5 and 7.5 mg/kg body weight (bw) DON for 8 days and the differential protein expressions in their blood plasma were determined by SELDI - Time-of-Flight/Mass Spectrometry (TOF/MS) and the immunoglobulins (Igs) G, A, M and E in the serum were investigated. 11.7 kDa protein was significantly highly expressed according to DON administration and this protein was purified by employing a methyl ceramic HyperD F column with using optimization buffer for adsorption and desorption. The purified protein was identified as a haptoglobin precursor by peptide mapping with using LC/Q-TOF/MS and MALDI-TOF/MS and this was confirmed by western blotting and ELISA. IgG and IgM in serum were decreased in a dose-dependent manner and IgA was decreased at 7.5 mg/kg bw DON administration, but the IgE level was not changed. To compare the expressions of haptoglobin and the Igs patterns between aflatoxin B1 (AFB1), zearalenone (ZEA) and DON intoxications, rats were orally administered with AFB1 1.0, ZEA 240 and DON 7.5 mg/kg bw for 8 days. Haptoglobin was increased only at DON 7.5 mg/kg bw, while it was slightly decreased at ZEA 240 mg/kg bw and it was not detected at all at AFB1 1.0 mg/kg bw. IgG and IgA were decreased by DON, but IgG, IgA, IgM and IgE were all increased by AFB1. No changes were observed by ZEA administration. These results show that plasma haptoglobin could be a diagnostic biomarker for DON intoxication when this is combined with examining the serum Igs.
Kim
Eun Joo
EJ
National Veterinary Research & Quarantine Service, Anyang 430-824, Korea.
Jeong
Sang Hee
SH
Cho
Joon Hyoung
JH
Ku
Hyun Ok
HO
Pyo
Hyun Mi
HM
Kang
Hwan Goo
HG
Choi
Kyoung Ho
KH
D016604:Aflatoxin B1 / Q000633:toxicity; D000818:Animals; D001798:Blood Proteins / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006242:Haptoglobins / Q000187:drug effects / Q000378:metabolism*; D007136:Immunoglobulins / Q000097:blood* / Q000187:drug effects; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D017208:Rats, Wistar; D014255:Trichothecenes / Q000633:toxicity*; D015025:Zearalenone / Q000633:toxicity
D001798:Blood Proteins; D006242:Haptoglobins; D007136:Immunoglobulins; D014255:Trichothecenes; D015025:Zearalenone; D016604:Aflatoxin B1; C007262:deoxynivalenol