Caveolae as potential mediators of MCH-signaling pathways.
The melanin-concentrating hormone receptor (MCHR) 1 is a G protein-coupled receptor involved in the regulation of appetite and energy expenditure in mammals. Here, we show that MCHR1 partitions to lipid rafts in stably expressing Chinese hamster ovary cells. In addition to co-fractionating with lipid rafts containing caveolin-1 on sucrose gradients, caveolin-1 was present in MCHR1 immunoprecipitates, suggesting that MCHR1 complexes with caveolae. The cholesterol-depleting drug methyl-beta-cyclodextrin impaired MCH-mediated ERK signaling. These data suggest that a functional interaction between MCHR1 and caveolin-1 in lipid rafts exists and provide a basis for further biochemical studies to understand the significance on MCH-mediated signal transduction events.
Cook
Laurie B
LB
Department of Biological Sciences, 217 Lennon Hall, The College at Brockport, State University of New York, 350 New Campus Drive, Brockport, NY 14420, USA. lcook@brockport.edu
Delorme-Axford
Elizabeth B
EB
Robinson
Kelsi
K
D000818:Animals; D016466:CHO Cells; D021941:Caveolae / Q000187:drug effects / Q000378:metabolism*; D051242:Caveolin 1 / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D047468:Immunoprecipitation; D021962:Membrane Microdomains / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D017481:Receptors, Somatostatin / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D047392:beta-Cyclodextrins / Q000494:pharmacology
D051242:Caveolin 1; C103263:MCHR1 protein, human; D017481:Receptors, Somatostatin; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3