title: Raised chondroitin sulphate WF6 epitope levels in gingival crevicular fluid in chronic periodontitis.
abstract: OBJECTIVE
  To determine the levels of chondroitin sulphate (CS) WF6 epitope, recognized by WF6 monoclonal antibody, in gingival crevicular fluid (GCF) from different stages of periodontal disease and healthy periodontium, and to correlate those levels with clinical parameters.
  
  
  METHODS
  GCF samples, collected from 389 sites, were analysed for the WF6 epitope levels by the competitive enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The median WF6 epitope level was significantly higher in chronic periodontitis sites (n=185) than in healthy and gingivitis sites (n=204) (p<0.001), whereas the median levels did not significantly differ between healthy (n=65) and gingivitis sites (n=139). The median level in severe periodontitis sites (n=60) was significantly higher than that in moderate periodontitis sites (n=63) (p=0.019). Similarly, the median level in moderate periodontitis sites was significantly higher than that in slight periodontitis sites (n=62) (p=0.001). The WF6 epitope levels significantly correlated with probing depth (r=0.777, p=0.001) and loss of clinical attachment level (r=0.814, p=0.001).
  
  
  CONCLUSIONS
  Elevated CS WF6 epitope levels in GCF are associated with severity of periodontitis. The WF6 antibody may therefore be clinically applied to monitor disease severity and progression.
authors: Department of Periodontology, Thailand Excellence Center for Tissue Engineerig, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors: Sakornrat
authors: S
authors: Khongkhunthian
authors: Napachakorn
authors: N
authors: Srimueang
authors: Suttichai
authors: S
authors: Krisanaprakornkit
authors: Komkham
authors: K
authors: Pattanaporn
authors: Siriwan
authors: S
authors: Ong-Chai
authors: Prachya
authors: P
authors: Kongtawelert
mesh_terms: D000328:Adult; D000368:Aged; D016301:Alveolar Bone Loss / Q000378:metabolism* / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002809:Chondroitin Sulfates / Q000276:immunology / Q000378:metabolism*; D055113:Chronic Periodontitis / Q000378:metabolism* / Q000473:pathology; D000939:Epitopes / Q000276:immunology; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D005891:Gingivitis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D010514:Periodontal Pocket / Q000378:metabolism / Q000473:pathology; D012016:Reference Values
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D000939:Epitopes; D002809:Chondroitin Sulfates