A rapid lateral flow stool antigen immunoassay and (14)C-urea breath test for the diagnosis and eradication of Helicobacter pylori infection in children.
Our aim was to evaluate diagnostic accuracy of rapid immunochromatographic stool antigen test (Rapid HpSA; LINEAR Chemical, Barcelona, Spain) and a practical low-dose (14)C urea breath test (UBT) (Heliprobetrade mark) test before and after eradication therapy. One hundred nine children with abdominal symptoms (age range, 5-17 years; mean, 12.1) underwent endoscopy, (14)C-UBT, and Rapid HpSA. Patients were defined as Hp infected when histology was positive for Hp. Forty children (36.6%) were Hp infected. The sensitivity of Rapid HpSA and (14)C-UBT was 65% and 92.5% (P = 0.0003), respectively; the specificity of Rapid HpSA and (14)C-UBT was 92.3% and 85.5% (P = 0.180), respectively. After eradication therapy endoscopy, (14)C-UBT and Rapid HpSA were repeated. The eradication rate was 70.5%. After eradication, the sensitivity of Rapid HpSA and (14)C-UBT was 60% and 100%, respectively; the specificity of Rapid HpSA and (14)C-UBT was 100%. (14)C-UBT was more reliable than the Rapid HpSA test for the diagnosis and for confirming eradication of Hp infection.
Kuloğlu
Zarife
Z
Pediatric Gastroenterology, School of Medicine, Ankara University, 06100 Ankara, Turkey. zarifekuloglu@yahoo.com
Kansu
Aydan
A
Kirsaçlioğlu
Ceyda Tuna
CT
Ustündağ
Gonca
G
Aysev
Derya
D
Ensari
Arzu
A
Küçük
Nuriye Ozlem
NO
Girgin
Nurten
N
D000293:Adolescent; D000658:Amoxicillin / Q000008:administration & dosage / Q000627:therapeutic use; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000627:therapeutic use; D000942:Antigens, Bacterial / Q000032:analysis*; D001944:Breath Tests / Q000379:methods; D002245:Carbon Dioxide; D002250:Carbon Radioisotopes; D002648:Child; D002675:Child, Preschool; D017291:Clarithromycin / Q000008:administration & dosage / Q000627:therapeutic use; D005243:Feces / Q000737:chemistry*; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D009853:Omeprazole / Q000008:administration & dosage / Q000627:therapeutic use; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D000897:Anti-Ulcer Agents; D000942:Antigens, Bacterial; D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D000658:Amoxicillin; D017291:Clarithromycin; D009853:Omeprazole