Fusion expression of mutated cecropin CMIV inE. coli.
A cDNA coding mutated cecropin CMIV fromBombyx mori was synthesized according to its amino acid sequence usingE. coli biased codons. The gene was cloned into the fusion expression vector pEZZ318 and was expressed inE. coli HB101. The fusion protein produced was purified by affinity chromatography to yield 26 mg/L fusion product. The anti-bacterial activities of recombinant cecropin CMIV were recovered after cleavage by chemical method.
Xie
W
W
State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, Nanjing University, 210093, Nanjing, China.
Qiu
Q
Q
Dong
X
X
Hua
Z
Z
Xu
X
X