Antigen changes of monoclonal antibody MSH27 in process of post-testicular maturation (in mice).
An anti-mouse spermatozoon monoclonal antibody, MSH27, as well as its purified antigen, can block sperm-egg membrane fusion. As a candidate protein for sperm-egg membrane fusion, the sperm antigen was investigated in the process of post-testicular maturation (PTM). The molecule was produced in testes and located on the plasma membrane of the postacrosomal area of the spermatozoon. However, the epitope recognized by the MSH27 (MSH27Ep) was not exposed until the occurrence of the acrosome reaction. In the process of fertilization, spermatozoa must complete the acrosome reaction before penetrating across the zona pellucidas (ZPs) to approach the plasma membrane of eggs. The effects of the acrosome reaction and penetration of the ZP on the exposure of the MSH27Ep were also studied. It was shown that the percentage of the spermatozoa with the MSH27Ep exposed increased followed with their mature status in PTM. In fact, it had a linear correlativity with the rate of the acrosome reaction. After spermatozoa had passed ZPs, almost all of them became the MSH27-positive in immuno-staining compared with only a part of the spermatozoa after the acrosome reaction. In a word, the exposure of MSH27Ep was coincident with attaining the ability to penetrate the plasma membrane of eggs.
Zhuang
D
D
Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Han
Z
Z
Song
X
X
Qi
Y
Y
Duan
C
C
Liu
H
H
Chen
D
D