Self-sampling for human papillomavirus (HPV) testing as cervical cancer screening option. Experience from the LAMS study.
OBJECTIVE
To compare Hybrid Capture II (HC2) in detecting high-risk (HR) HPV in patient-collected vaginal samples with those obtained using gynaecologist collected samples.


METHODS
Patients were submitted to Pap smears, visual inspection with acetic acid (VIA) and HC2 for hr-HPV.


RESULTS
A total of 1,081 HC2 tests for HR-HPV were performed: 770 (71.2%) samples were collected by a physician and 311 (28.8%) were self-collected by the patients. In detecting any cervical lesion, the sensitivity of HC2 collected by a physician was higher (92.86%) than that (37.5%) in the self-sampling group. Negative predictive value (NPV) was high for both, 99.69% and 93.75%, respectively. Using the CIN2 cutoff, performance of HC2 was significantly improved: 92.9% and 62.5%, respectively. HC2 specificity for any cervical lesion and for CIN2 or higher were close to 90% in both groups.


CONCLUSIONS
Self-sampled HPV testing is a powerful option to increase the detection of cervical lesions in women segregated from prevention programs.
Longatto-Filho
A
A
Instituto Adolfo Lutz, São Paulo, Brazil. longatto@ecsaude.uminho.pt
Roteli-Martins
C
C
Hammes
L
L
Etlinger
D
D
Pereira
S M Miranda
SM
Erzen
M
M
Branca
M
M
Naud
P
P
Derchain
S F M
SF
Sarian
L O
LO
Matos
J
J
Gontijo
R
R
Lima
T
T
Maeda
M Y S
MY
Tatti
S
S
Syrjänen
S
S
Syrjänen
K
K
D000328:Adult; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007843:Latin America; D008403:Mass Screening / Q000379:methods; D008875:Middle Aged; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D011237:Predictive Value of Tests; D015995:Prevalence; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D016353:Self-Examination / Q000379:methods; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000517:prevention & control* / Q000821:virology; D014626:Vaginal Smears / Q000379:methods*; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000517:prevention & control / Q000821:virology
D011933:Reagent Kits, Diagnostic