Direct detection of Kitasatospora species with a chaperone oligonucleotide microarray method lacking PCR amplification.
Identification of members of the genus Kitasatospora from soil samples has been introduced to evaluate occurrence of potential natural compound producers in different habitats. The microarray hybridization usually involves PCR amplification of the target DNA. Since PCR might lead to biased amplification, a diagnostic Kitasatospora microarray technique was improved by a protocol lacking PCR amplification prior to hybridization. The described advanced hybridization method used chaperone oligonucleotides for direct co-hybridization with genomic DNA on an oligonuclotide microarray with optical readout.
Guenther
Sebastian
S
Leibniz Institute for Natural Product Research and Infection Biology - Hans-Knöll-Institute, Jena, Germany. guenther.sebastian@vetmed.fu-berlin.de
Groth
Ingrid
I
Schierack
Peter
P
Grabley
Susanne
S
Munder
Thomas
T
D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D012988:Soil Microbiology; D013306:Streptomycetaceae / Q000235:genetics / Q000302:isolation & purification*
D015345:Oligonucleotide Probes