Cryopreservation has no effect on meiotic recombination and synapsis in testicular tissues.
The effects of cryopreservation on meiotic progression, synapsis, recombination, and structure of synaptonemal complexes (SCs) in testicular tissues were evaluated by comparing the above-mentioned parameters in frozen and fresh testicular tissues from the same men. No differences in meiotic progression, the mean number of MLH1 foci per cell, the mean number of autosomal SCs with different numbers of MLH1 foci, or the fidelity of the synapsis were observed between fresh and frozen testicular tissues.
Li
Jianhua
J
Laboratory of Molecular and Cell Genetics, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.
Leng
Mei
M
Ma
Tieliang
T
Yu
Dexin
D
Shi
Haoqiang
H
Shi
Qinghua
Q
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000328:Adult; D000368:Aged; D023902:Chromosome Pairing / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D008297:Male; D008540:Meiosis / Q000502:physiology*; D008875:Middle Aged; D000070957:MutL Protein Homolog 1; D009687:Nuclear Proteins / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism; D013573:Synaptonemal Complex / Q000502:physiology; D013737:Testis / Q000166:cytology* / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; C501486:MLH1 protein, human; D009687:Nuclear Proteins; D000070957:MutL Protein Homolog 1