Induction of HIV-1 MPR(649-684)-specific IgA and IgG antibodies in caprine colostrum using a peptide-based vaccine.
Induction of antigen-specific antibodies against HIV-1 in colostrum and milk may help prevent breast milk transmission of the virus. A peptide vaccine against the HIV-1 gp41 membrane proximal region (MPR(649-684)) was evaluated as proof-of-principle in a caprine model. Pregnant Alpine/Saanen goats were immunized with MPR(649-684) peptide conjugated to KLH using alum adjuvant. Immunizations were intramuscular, intranasal, and in the supramammary lymph node region. Samples collected after parturition demonstrated the presence of MPR(649-684)-specific antibodies in colostrum and serum. These results support the concept that a peptide vaccine can effectively induce MPR(649-684)-specific sIgA and IgG in the colostrum of a lactating species.
Dorosko
Stephanie M
SM
Department of Microbiology & Immunology, Dartmouth Medical School, 1 Medical Center Drive, Lebanon, NH 03756, USA. Stephanie.M.Dorosko@dartmouth.edu
Ayres
Sandra L
SL
Connor
Ruth I
RI
D016915:AIDS Vaccines / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000534:Alum Compounds / Q000494:pharmacology; D000818:Animals; D003126:Colostrum / Q000276:immunology* / Q000821:virology; D005260:Female; D006041:Goats; D015483:HIV Antibodies / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control; D015497:HIV-1 / Q000276:immunology*; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control; D009500:Neutralization Tests; D011247:Pregnancy; D022223:Vaccines, Subunit / Q000276:immunology
D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D015483:HIV Antibodies; D015700:HIV Envelope Protein gp41; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022223:Vaccines, Subunit; C514787:gp41 protein, Human immunodeficiency virus 1; C041524:aluminum sulfate