Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells.
The specific role of size scale, surface capping, and aspect ratio of zinc oxide (ZnO) particles on toxicity toward prokaryotic and eukaryotic cells was investigated. ZnO nano and microparticles of controlled size and morphology were synthesized by wet chemical methods. Cytotoxicity toward mammalian cells was studied using a human osteoblast cancer cell line and antibacterial activity using Gram-negative bacteria (Escherichia coli) as well as using Gram-positive bacteria (Staphylococcus aureus). Scanning electron microscopy (SEM) was conducted to characterize any visual features of the biocidal action of ZnO. We observed that antibacterial activity increased with reduction in particle size. Toxicity toward the human cancer cell line was considerably higher than previously observed by other researchers on the corresponding primary cells, suggesting selective toxicity of the ZnO to cancer cells. Surface capping was also found to profoundly influence the toxicity of ZnO nanoparticles toward the cancer cell line, with the toxicity of starch-capped ZnO being the lowest. Our results are found to be consistent with a membrane-related mechanism for nanoparticle toxicity toward microbes.
Nair
Shantikumar
S
Amrita Center for Nanosciences, Amrita Vishwa Vidyapeetham, Kochi 682 026, Kerala, India. shantinair@aims.amrita.edu
Sasidharan
Abhilash
A
Divya Rani
V V
VV
Menon
Deepthy
D
Nair
Seema
S
Manzoor
K
K
Raina
Satish
S
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects*; D001859:Bone Neoplasms / Q000188:drug therapy / Q000473:pathology*; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D014407:Tumor Cells, Cultured; D015034:Zinc Oxide / Q000737:chemistry* / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D003603:Cytotoxins; D011092:Polyethylene Glycols; D015034:Zinc Oxide