title: Quantitative and qualitative analysis of proteins in fresh frozen plasma obtained from whole blood donations and prepared with two photochemical treatments.
abstract: Thirty-six fresh frozen plasma (FFP) units obtained from whole blood donations were used for 12 replicate experiments. For each replicate experiment, three ABO-matched FFP units were pooled and divided into three units containing different volumes of identical plasma. One unit was used as control FFP, one unit was treated with methylene blue plus visible light and one unit was treated with amotosalen and UVA light. The overall coagulation factor levels were better maintained in untreated FFP than in photochemically treated plasma. However, treated-plasma by both photochemical methods maintained coagulation factor levels that met or exceeded the European Pharmacopeia requirements for therapeutic plasma.
authors: Banc de Sang i Teixits, Barcelona, Spain. jcid.hj23.ics@gencat.cat
authors: Joan
authors: J
authors: Cid
authors: Laia
authors: L
authors: Ramiro
authors: Nuria
authors: N
authors: Martínez
authors: Marta
authors: M
authors: Palomo
authors: Olga
authors: O
authors: Magallon
authors: Montse
authors: M
authors: Claparols
authors: Pilar
authors: P
authors: Ortiz
authors: Lluis
authors: L
authors: Puig
authors: Ramon Pau
authors: RP
authors: Pla
mesh_terms: D001779:Blood Coagulation Factors / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D017848:Blood-Borne Pathogens / Q000528:radiation effects; D005340:Fibrinogen / Q000032:analysis; D011564:Furocoumarins / Q000494:pharmacology / Q000528:radiation effects; D006801:Humans; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology* / Q000528:radiation effects; D010777:Photochemistry; D010949:Plasma / Q000737:chemistry* / Q000187:drug effects; D014466:Ultraviolet Rays; D038301:Virus Inactivation; D014841:von Willebrand Factor / Q000032:analysis
chemical_list: D001779:Blood Coagulation Factors; D001798:Blood Proteins; D011564:Furocoumarins; D014841:von Willebrand Factor; D005340:Fibrinogen; C118577:amotosalen; D008751:Methylene Blue