Bacterial cell wall products increases stabilization of HIF-1 alpha in an oligodendrocyte cell line preconditioned by cobalt chloride or desferrioxamine.
We examined the effect of lipopolysaccharide (LPS) or lipotechoic acid (LTA) on the regulation of hypoxia inducible factor (HIF-1) alpha on the MO3.13 cells, a human oligodendroglial cell line. Our study shows that MO3.13 cells express the toll like receptors (TLR's) but do not increase cellular levels of HIF-1 alpha following exposure to bacterial cell wall products. When MO3.13 cells were preconditioned by desferrioxamine (DFO) or cobalt chloride (CoCl(2)) and then treated with either LPS or LTA, HIF-1 alpha levels were higher than that induced by DFO or CoCl(2) alone. The increase in HIF-1 alpha was due to increased protein stability that was mediated by activation of the ERK-MAP kinase pathway.
Yao
Song-yi
SY
Department of Neurology, Multiple Sclerosis Research Center, Vanderbilt University Medical Center, Nashville, Tennessee 37212, USA.
Soutto
Mohammed
M
Sriram
Subramaniam
S
D016587:Antimutagenic Agents / Q000494:pharmacology*; D001419:Bacteria / Q000166:cytology; D002461:Cell Line, Transformed; D002473:Cell Wall / Q000737:chemistry; D003035:Cobalt / Q000494:pharmacology*; D003676:Deferoxamine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009836:Oligodendroglia / Q000187:drug effects*; D017262:Siderophores / Q000494:pharmacology*; D013682:Teichoic Acids / Q000494:pharmacology; D013997:Time Factors; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism
D016587:Antimutagenic Agents; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008070:Lipopolysaccharides; D017262:Siderophores; D013682:Teichoic Acids; D051193:Toll-Like Receptors; D003035:Cobalt; C009900:lipoteichoic acid; C018021:cobaltous chloride; D003676:Deferoxamine