TiO2 nanowire bundle microelectrode based impedance immunosensor for rapid and sensitive detection of Listeria monocytogenes.
A novel TiO 2 nanowire bundle microelectrode based immunosensor was demonstrated as a more sensitive, specific, and rapid technology for detection of Listeria monocytogenes. TiO 2 nanowire bundle was prepared through a hydrothermal reaction of alkali with TiO 2 powder and connected to gold microelectrodes with mask welding. Monoclonal antibodies were immobilized on the surface of a TiO 2 nanowire bundle to specifically capture L. monocytogenes. Impedance change caused by the nanowire-antibody-bacteria complex was measured and correlated to bacterial number. This nanowire bundle based immunosensor could detect as low as 10 (2) cfu/ml of L. monocytogenes in 1 h without significant interference from other foodborne pathogens.
Wang
Ronghui
R
Department of Biological and Agricultural Engineering, Cell and Molecular Biology Program, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, Arkansas 72701, USA.
Dong
Wenjun
W
Ruan
Chuanmin
C
Kanayeva
Damira
D
Tian
Ryan
R
Lassiter
Kentu
K
Li
Yanbin
Y
D015374:Biosensing Techniques; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D053770:Nanowires; D014025:Titanium / Q000737:chemistry*
C009495:titanium dioxide; D014025:Titanium