Raised chondroitin sulphate WF6 epitope levels in gingival crevicular fluid in chronic periodontitis.
OBJECTIVE
To determine the levels of chondroitin sulphate (CS) WF6 epitope, recognized by WF6 monoclonal antibody, in gingival crevicular fluid (GCF) from different stages of periodontal disease and healthy periodontium, and to correlate those levels with clinical parameters.


METHODS
GCF samples, collected from 389 sites, were analysed for the WF6 epitope levels by the competitive enzyme-linked immunosorbent assay.


RESULTS
The median WF6 epitope level was significantly higher in chronic periodontitis sites (n=185) than in healthy and gingivitis sites (n=204) (p<0.001), whereas the median levels did not significantly differ between healthy (n=65) and gingivitis sites (n=139). The median level in severe periodontitis sites (n=60) was significantly higher than that in moderate periodontitis sites (n=63) (p=0.019). Similarly, the median level in moderate periodontitis sites was significantly higher than that in slight periodontitis sites (n=62) (p=0.001). The WF6 epitope levels significantly correlated with probing depth (r=0.777, p=0.001) and loss of clinical attachment level (r=0.814, p=0.001).


CONCLUSIONS
Elevated CS WF6 epitope levels in GCF are associated with severity of periodontitis. The WF6 antibody may therefore be clinically applied to monitor disease severity and progression.
Khongkhunthian
Sakornrat
S
Department of Periodontology, Thailand Excellence Center for Tissue Engineerig, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Srimueang
Napachakorn
N
Krisanaprakornkit
Suttichai
S
Pattanaporn
Komkham
K
Ong-Chai
Siriwan
S
Kongtawelert
Prachya
P
D000328:Adult; D000368:Aged; D016301:Alveolar Bone Loss / Q000378:metabolism* / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002809:Chondroitin Sulfates / Q000276:immunology / Q000378:metabolism*; D055113:Chronic Periodontitis / Q000378:metabolism* / Q000473:pathology; D000939:Epitopes / Q000276:immunology; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D005891:Gingivitis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D010514:Periodontal Pocket / Q000378:metabolism / Q000473:pathology; D012016:Reference Values
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D000939:Epitopes; D002809:Chondroitin Sulfates