title: Validation and application of an HPLC method for determination of di (2-ethylhexyl) phthalate and mono (2-ethylhexyl) phthalate in liver samples.
abstract: A reversed-phase gradient elution, UV detection method is developed for the simultaneous determination of mono (2-ethylhexyl) phthalate [MEHP] and di (2-ethylhexyl) phthalate [DEHP] in tissue samples. The method is validated with respect to extraction recovery, inter and intra-day precision, linearity of response, detect ability, and specificity. The validated method has been successfully applied to the study of DEHP and MEHP in liver, kidney, testis, brain, and plasma samples from rat.
authors: Department of Chemistry, Northeastern Illinois University, Chicago, IL 60625, USA. debajit@uic.edu
authors: Debajit
authors: D
authors: Sircar
authors: Sargon J
authors: SJ
authors: Albazi
authors: Yousef
authors: Y
authors: Atallah
authors: William
authors: W
authors: Pizzi
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013737:Testis / Q000737:chemistry; D035501:Uncertainty
chemical_list: D004051:Diethylhexyl Phthalate; C016599:mono-(2-ethylhexyl)phthalate