Intrinsic Raman spectroscopy for quantitative biological spectroscopy part I: theory and simulations.
We present a novel technique, intrinsic Raman spectroscopy (IRS), to correct turbidity-induced Raman spectral distortions, resulting in the intrinsic Raman spectrum that would be observed in the absence of scattering and absorption. We develop an expression relating the observed and intrinsic Raman spectra through diffuse reflectance using the photon migration depiction of light transport. Numerical simulations are employed to validate the theoretical results and study the dependence of this expression on sample size and elastic scattering anisotropy.
Shih
Wei-Chuan
WC
GR Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Bechtel
Kate L
KL
Feld
Michael S
MS
D001704:Biopolymers / Q000737:chemistry*; D003198:Computer Simulation; D008027:Light; D008954:Models, Biological; D008956:Models, Chemical; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000379:methods*
D001704:Biopolymers