Effects of a new allograft processing procedure on graft healing in a canine model: a preliminary study.
Graft healing in vivo can be affected by allograft processing. We asked whether a new processing technique influenced graft-host healing compared with autograft and a standard processing technique in a canine ulna model. We used bilateral intercalary allografts or autografts in the ulna of 13 skeletally mature male coonhounds. Each animal received two allografts, either one autograft and one allograft, or two autografts. At term (90 days), the graft sites were harvested. We assessed union with high-resolution xray imaging. Each specimen was processed for nondecalcified histologic analysis to assess the graft-host interface. Quantitative histomorphometric analysis was performed to determine spatial location and area of bone. Radiographic analysis, histologic analysis, and histomorphometric measures revealed no differences in union, mean total bone area, or total endosteal/intramedullary bone for the new process, standard process, and autografts. Our preliminary data suggest the new processing techniques may increase the safety of allograft transplantation without adversely affecting union when compared with standard processing techniques and autograft in a canine model.
Beebe
Kathleen S
KS
Department of Orthopaedics, UMDNJ-New Jersey Medical School, Newark, NJ 07103, USA. beebeka@umdnj.edu
Benevenia
Joseph
J
Tuy
Benjamin E
BE
DePaula
C Alex
CA
Harten
Robert D
RD
Enneking
William F
WF
D000818:Animals; D016025:Bone Transplantation; D004285:Dogs; D017102:Fracture Healing; D008297:Male; D023421:Models, Animal; D010865:Pilot Projects; D011859:Radiography; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D014458:Ulna Fractures / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*