title: Molecular and ultrastructural characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells.
abstract: In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (IGF-1). A panel of cellular and molecular markers was used for characterization of the endothelial cells. The cells were strongly positive for von Willebrand factor (vWF) and vascular endothelial growth factor receptor 2 (VEGFR2) when measured at protein and mRNA levels. Development of endothelial cells was found to be associated with formation of typical organelles such as Weibel Palade (WP) bodies, Cavealae and pinocytic vesicles. Early vessel growth was also evidenced by showing specific junctions between the cells. The migratory and angiogenic properties of the cells were confirmed by showing capillary network formation in vitro. These results indicate that the capacity of endothelial cells differentiated from hBMSCs in formation of vascular system is consistent with molecular and structural development.
authors: Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
authors: Maryam
authors: M
authors: Jazayeri
authors: Abdolamir
authors: A
authors: Allameh
authors: Masoud
authors: M
authors: Soleimani
authors: Seyed Hamid
authors: SH
authors: Jazayeri
authors: Abbas
authors: A
authors: Piryaei
authors: Somaieh
authors: S
authors: Kazemnejad
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D008875:Middle Aged; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000378:metabolism; D014841:von Willebrand Factor / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D042461:Vascular Endothelial Growth Factor A; D014841:von Willebrand Factor; D007334:Insulin-Like Growth Factor I; D040301:Vascular Endothelial Growth Factor Receptor-2