title: Culture medium study of human mesenchymal stem cells for practical use of tissue engineering and regenerative medicine.
abstract: Mesenchymal stem cells (MSCs) are multipotent cells that have potential to differentiate into various phenotypes and appear useful for therapeutic applications in regenerative medicine. Mesenchymal Stem Cell Basal Medium (MSCBM; Cambrex) is a widespread and suitable medium used in MSC cultivation, but it is extremely difficult to use generally for clinical treatment because of its unclear traceability and cost. Assessment of cost-effectiveness is a critical issue for successful practical application; therefore, we have evaluated the effects of a generally used medium, Dulbecco's Modified Eagle's Medium (D-MEM) on the expansion of MSCs in comparison with MSCBM. To isolate human MSCs, bone marrow aspirates were taken and cultured in MSCBM or D-MEM. Proliferation assay indicated that MSCs isolated in both media showed a similar growth rate. When supplemented with osteo-inductive reagents, alkaline phosphatase activity was not significantly different between cells in D-MEM and MSCBM. Moreover, the cells expressed identical mesenchymal lineage markers, but not endothelial and hematopoietic lineage markers. Our findings suggest that cells obtained from bone marrow and cultured in D-MEM might possess proliferative capacity and the potential to differentiate into an osteogenic lineage. In conclusion, D-MEM might be a suitable basal medium for the cultivation of MSCs for clinical applications.
authors: Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors: Sayaka
authors: S
authors: Nakamura
authors: Yoichi
authors: Y
authors: Yamada
authors: Shunsuke
authors: S
authors: Baba
authors: Harumi
authors: H
authors: Kato
authors: Hiroyuki
authors: H
authors: Kogami
authors: Makoto
authors: M
authors: Takao
authors: Naoyuki
authors: N
authors: Matsumoto
authors: Minoru
authors: M
authors: Ueda
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D003114:Colony-Forming Units Assay; D003470:Culture Media; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010011:Osteocytes / Q000166:cytology / Q000502:physiology; D010012:Osteogenesis; D044968:Regenerative Medicine / Q000379:methods; D023822:Tissue Engineering / Q000379:methods
chemical_list: D015415:Biomarkers; D003470:Culture Media