[Effect of coking oven emissions on level of serum oxidation-reduction and peripheral white blood cellular ras P21 and P53 in coke oven workers].
OBJECTIVE
To explore the adverse effects of coking oven emissions (COE) on the serum oxidation-reduction and relevant genes in the exposed workers.


METHODS
Fifty-six coke oven workers and forty controls were investigated. Serum Malondialdehyde (MDA) levels and the activities of total superoxide dismutases (T-SOD) were measured by spectrophotometrical method. Immunohistochemical method was used to assess the P21 and P53 levels in peripheral white blood cells.


RESULTS
Compared with controls, the individuals exposed to COE had significantly increased levels of serum MDA [(5.30 +/- 2.29) nmol/mL, P < 0.01] and markedly decreased levels of T-SOD [(100.04 +/- 10.75) NU/mL]. Additionally, the median levels of P53 and P21 were markedly increased in the exposed individuals compared with the controls (21.4% and 23.2%, respectively, all P < 0.05).


CONCLUSIONS
The findings indicate that occupational exposure to COE causes the rise of serum oxidation-reduction MDA and the fall of T-SOD, and increasing expression levels of P21 and P53 proteins before the occurrence of apparent clinical symptoms.
Zhang
Qiao
Q
Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, China.
Zhou
Fang
F
Yao
Wu
W
Zhao
Yi-bo
YB
Li
Zhi-yuan
ZY
Xu
Yu-bao
YB
Wu
Yi-ming
YM
D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects; D016022:Case-Control Studies; D003077:Coke / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects; D010084:Oxidation-Reduction; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000097:blood; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D055815:Young Adult
D000395:Air Pollutants, Occupational; D003077:Coke; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)