Exploration of the genes responsible for unlimited proliferation of immortalized lung fibroblasts.
Regulation mechanism of lung fibroblast proliferation remains unknown. To elucidate the key molecules in it, the authors here established mortal and immortal nontransformed lung fibroblast cell line/strains with elongated life span by telomerase reverse transcriptase gene transfection. Comparing the expression profiles of them, 51 genes were explored to be the candidates responsible for regulation of cellular proliferation of lung fibroblasts. This set of fibrobrast strains of same origin with different proliferative capacities may become useful model cells for research on lung fibroblast growth regulation and the candidate genes explored in this study may provide biomarkers or therapeutic targets of pulmonary fibrosis.
Hiyama
Keiko
K
Department of Translational Cancer Research, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan. khiyama@hiroshima-u.ac.jp
Tanimoto
Keiji
K
Nishimura
Youhei
Y
Tsugane
Momomi
M
Fukuba
Ikuko
I
Sotomaru
Yusuke
Y
Hiyama
Eiso
E
Nishiyama
Masahiko
M
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D002472:Cell Transformation, Viral / Q000235:genetics; D005347:Fibroblasts / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008168:Lung / Q000196:embryology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009374:Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D011003:Ploidies; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D016615:Telomere / Q000235:genetics; D013997:Time Factors; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; C509186:TERT protein, human; D019098:Telomerase