title: Endogenous oxalogenesis after acute intravenous loading with ethylene glycol or glycine in rats receiving standard and vitamin B6-deficient diets.
abstract: OBJECTIVE
  The effect on endogenous oxalate synthesis of acute intravenous loading with ethylene glycol or glycine was investigated in rats on a standard or a vitamin B6-deficient diet.
  
  
  METHODS
  Twenty-four male Wistar rats weighing approximately 180 g were randomly divided into ethylene glycol and glycine groups of 12 animals each. These groups were further divided into two subgroups of six animals each that were fed either a standard or a vitamin B6-deficient diet for 3 weeks. Animals of these two subgroups received an intravenous infusion of 20 mg (322.22 micromol) of ethylene glycol or 100 mg (1332.09 micromol) of glycine, respectively. Urine samples were collected just before intravenous infusion of each substance and at hourly intervals until 5 h after receiving the infusion. Urinary oxalate, glycolate, and citrate levels were measured by capillary electrophoresis.
  
  
  RESULTS
  Urinary oxalate and glycolate excretion was significantly increased after ethylene glycol administration. Significant differences between the control and vitamin B6-deficient groups were found. In contrast, there were only small changes of oxalate and glycolate excretion after glycine administration. Recovery of the given dose of ethylene glycol as oxalate in 5-h urine was 0.31% and 7.15% in the control and vitamin B6-deficient groups, respectively, whereas recovery of glycolate was 0.68% and 7.22%, respectively.
  
  
  CONCLUSIONS
  Ethylene glycol loading has a significant effect on urinary oxalate excretion in both normal and vitamin B6-deficient rats, whereas glycine loading only has a small effect. Oxalate and glycolate excretion after ethylene glycol loading were respectively 23-fold and 11-fold higher in vitamin B6-deficient rats than in controls.
authors: Division of Urology, Department of Organ-oriented Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
authors: Yaovalak
authors: Y
authors: Teerajetgul
authors: Rayhan Zubair
authors: RZ
authors: Hossain
authors: Noriko
authors: N
authors: Machida
authors: Kimio
authors: K
authors: Sugaya
authors: Yoshihide
authors: Y
authors: Ogawa
mesh_terms: D000818:Animals; D004032:Diet; D019855:Ethylene Glycol / Q000008:administration & dosage*; D005998:Glycine / Q000008:administration & dosage*; D007262:Infusions, Intravenous; D008297:Male; D010070:Oxalates / Q000652:urine*; D051381:Rats; D017208:Rats, Wistar; D025101:Vitamin B 6 / Q000008:administration & dosage*; D014803:Vitamin B Complex / Q000008:administration & dosage*
chemical_list: D010070:Oxalates; D014803:Vitamin B Complex; D025101:Vitamin B 6; D019855:Ethylene Glycol; D005998:Glycine