title: Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp.
abstract: Necrotizing hepatopancreatitis (NHP) is considered to be one of the most important bacterial diseases affecting penaeid shrimp culture and is caused by an unclassified Gram-negative, pleomorphic, intracellular Alphaproteobacterium. Due to the enteric nature of the bacteria, PCR is the one non-lethal method available for detection of the pathogen. Over a decade ago, a PCR protocol was developed for detection of NHP, which over the subsequent years was shown to occasionally generate false positive reactions. The University of Arizona Aquaculture Pathology Laboratory has developed a set of primers and PCR cycling parameters that have been tested on a variety of DNA templates, using 2 types of PCR reagent systems, which eliminated the generation of false positive amplicons.
authors: Department of Veterinary Sciences and Microbiology, University of Arizona, Tucson, Arizona 85721, USA. lmn@email.arizona.edu
authors: Linda M
authors: LM
authors: Nunan
authors: Carlos
authors: C
authors: Pantoja
authors: Donald V
authors: DV
authors: Lightner
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D043143:Hepatopancreas / Q000382:microbiology; D033561:Penaeidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity