The involvement of sortase A in high virulence of STSS-causing Streptococcus suis serotype 2.
Sortase A (SrtA), originally identified as a transpeptidase in Staphylococcus aureus, plays key roles in full virulence of pathogenic bacteria. In silico genome-wide search suggested a srtA homologue from 05ZYH33, a Chinese human isolate of streptococcal toxic shock syndrome (STSS)-causing Streptococcus suis serotype 2 (S. suis 2, SS2). An isogenic srtA mutant (DeltasrtA) of 05ZYH33 strain was obtained by homologous recombination. Immunofluorescence analysis revealed that two known virulence-associated surface proteins featuring Leu-Pro-X-Thr-Gly motif, muramidase-released protein and surface antigen one, were absent in the DeltasrtA. Piglet infection experiments showed that deletion of srtA attenuated the full virulence of 05ZYH33 strain, and impaired its colonizing potential in specific organs. Furthermore, the DeltasrtA displayed significant reduction in adherence to human cells (Hep-2 and human umbilical vein endothelial cells). Collectively, we concluded that SrtA was involved in the virulence manifestation of STSS-causing SS2.
Wang
Changjun
C
Department of Pathogen Biology, Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing Medical University, Nanjing, China.
Li
Ming
M
Feng
Youjun
Y
Zheng
Feng
F
Dong
Yaqing
Y
Pan
Xiuzhen
X
Cheng
Gong
G
Dong
Ruiping
R
Hu
Dan
D
Feng
Xiaodan
X
Ge
Junchao
J
Liu
Di
D
Wang
Jing
J
Cao
Min
M
Hu
Fuquan
F
Tang
Jiaqi
J
D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D042783:Endothelial Cells / Q000382:microbiology; D006801:Humans; D011897:Random Allocation; D012772:Shock, Septic / Q000382:microbiology*; D013290:Streptococcal Infections / Q000382:microbiology*; D017011:Streptococcus suis / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D013552:Swine; D014774:Virulence
D001426:Bacterial Proteins; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases