title: Development of a biochip using antibody-coated gold nanoparticles to detect specific bioparticles.
abstract: This study developed a method of detecting bioparticles such as Salmonella that exist in the biological samples. The method employed a substrate with interlaced comb-like electrodes into which the mixtures of biological samples and antibody-coated gold nanoparticles were added. The alternative signals with appropriate frequency bands were then conducted into the comb-like electrodes to change the dielectrophoresis force. The gold-modified Salmonella can be adsorbed on the edges of the electrodes and isolated from various biological samples. The impedance of the adsorbed Salmonella on the edges of the electrodes was measured and comparison of the impedance between the electrodes with and without Salmonella can quantify the amount of the adsorbed Salmonella.
authors: Institute of Mechatronics Engineering, National Taipei University of Technology, and Taiwan Adventist Hospital, 1, Sec. 3, Zhong Xiao E. Rd., Taipei, 106, Taiwan. jthuang@ntut.edu.tw
authors: Jung-Tang
authors: JT
authors: Huang
authors: Shao-Yi
authors: SY
authors: Hou
authors: Shiuh-Bin
authors: SB
authors: Fang
authors: Hua-Wei
authors: HW
authors: Yu
authors: Hung-Chan
authors: HC
authors: Lee
authors: Chu-Zen
authors: CZ
authors: Yang
mesh_terms: D000906:Antibodies / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012475:Salmonella / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000906:Antibodies; D006046:Gold