title: A photolabile ligand for light-activated release of caged copper.
abstract: A photosensitive caged copper complex has been prepared from a tetradentate ligand (H2cage) composed of two pyridyl-amide arms connected by a photoreactive nitrophenyl group. H2cage binds Cu2+ in aqueous solution with a stability constant (log beta) of 10.8, which corresponds to a KD of 16 pM at pH 7.4. The neutral Cu2+ complex, [Cu(OH2)(cage)], crystallizes as a distorted trigonal bipyramid coordinated by two amide and two pyridyl N atoms, with a water molecule bound in the trigonal plane. Photolysis with 350 nm UV light cleaves the ligand backbone to release photoproducts with significantly diminished affinity for Cu2+, thereby uncaging the metal ion. When coordinated as the caged complex, copper has diminished reactivity to produce hydroxyl radicals from Fenton-like reaction mixtures containing hydrogen peroxide and ascorbic acid. Postphotolysis, uncaged copper promotes hydroxyl radical formation under the same conditions. The strategy of caging copper is promising for applications where light could be used to trigger release of copper as a pro-oxidant to increase oxidative stress or as a tool to release copper intracellularly to study mechanisms of copper trafficking.
authors: Department of Chemistry, Duke University, P.O. Box 90346, Durham, North Carolina 27708, USA.
authors: Katie L
authors: KL
authors: Ciesienski
authors: Kathryn L
authors: KL
authors: Haas
authors: Marina G
authors: MG
authors: Dickens
authors: Yohannes T
authors: YT
authors: Tesema
authors: Katherine J
authors: KJ
authors: Franz
mesh_terms: D000577:Amides / Q000138:chemical synthesis / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008024:Ligands; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010084:Oxidation-Reduction; D010782:Photolysis; D011725:Pyridines / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000577:Amides; D008024:Ligands; D009942:Organometallic Compounds; D011725:Pyridines; D003300:Copper