Expressional regulation in vitro of psbA mRNA 3' untranslated region in rice and sorghum.
To study the relationship between the structures of rice and sorghum psbA 3' UTR and gene expressional activity, six chimeric luc genes that encode luciferase under control of rice or sorghum psbA 5' UTR and 3' UTR or only 5' UTR were constructed. The levels of LUC accumulation inE. coli and the transcript stability in soluble protein extracts of rice, sorghum chloroplast and E. coli were examined respectively, and a detailed analysis about the function of these two 3' UTR has been carried out. Here the regulation effect of these 3' UTR are reported: (i) When having the same promoter, the chimeric genes with rice 3' UTR produce LUC much more than that with sorghum 3' UTR; (ii) elimination of 3' UTR results in the fluctuation of LUC accumulation no matter whether it is under control of rice or sorghum psbA 5' UTR; (iii) rice psbA 3' UTR exhibits a greater effect on stabilizing transcripts; (iv) psbA 3' IR-RNAs are more stable in chloroplast protein extracts than in E.coli protein extracts.
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Institute of Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Wu
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