title: Evaluation of a multianalyte profiling assay and an enzyme-linked immunosorbent assay for serological examination of Epstein-Barr virus-specific antibody responses in diagnosis of nasopharyngeal carcinoma.
abstract: Assessment of antibody responses to Epstein-Barr virus (EBV) antigens has been used to assist in nasopharyngeal carcinoma (NPC) diagnosis by several methods. In this study, we evaluated an in-house Luminex multianalyte profiling (xMAP) technology and commercial enzyme-linked immunosorbent assay (ELISA) kits for serological examination of EBV-specific antibody responses in 135 NPC patients and 130 healthy controls. Four EBV biomarkers were measured: immunoglobulin A (IgA) against viral capsid antigen (VCA), EBV nuclear antigen 1 (EBNA1), diffused early antigen (EA-D), and IgG against EA-D. The sensitivities and specificities of the four markers ranged between 71.5 and 90% for xMAP assays and 80 and 92% for ELISA. Logistic regression analysis revealed that the combined markers in the xMAP assay had overall sensitivity and specificity values of 82% and 92%, respectively. The correlation coefficient (r) values for the xMAP assay and ELISA were lowest for IgA-VCA (0.468) and highest for IgA-EBNA1 (0.846); for IgA-EA-D and IgG-EA-D, the r values were 0.719 and 0.798, respectively. The concordances of the two methods for NPC discrimination were good (79 to 88%). Our results suggest that both the xMAP assay and ELISA are satisfactory for EBV antibody evaluation when multiple antigens are included.
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Ai-Di
authors.initials: AD
authors.lastname: Gu
authors.forename: Hao-Yuan
authors.initials: HY
authors.lastname: Mo
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Xie
authors.forename: Rou-Jun
authors.initials: RJ
authors.lastname: Peng
authors.forename: Jin-Xin
authors.initials: JX
authors.lastname: Bei
authors.forename: Juan
authors.initials: J
authors.lastname: Peng
authors.forename: Miao-Yan
authors.initials: MY
authors.lastname: Li
authors.forename: Li-Zhen
authors.initials: LZ
authors.lastname: Chen
authors.forename: Qi-Sheng
authors.initials: QS
authors.lastname: Feng
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Jia
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Zeng