title: The interferon antagonist ML protein of thogoto virus targets general transcription factor IIB.
abstract: The ML protein of Thogoto virus, a tick-transmitted orthomyxovirus, is a splice variant of the viral matrix protein and antagonizes the induction of antiviral type I interferon (IFN). Here we identified the general RNA polymerase II transcription factor IIB (TFIIB) as an ML-interacting protein. Overexpression of TFIIB neutralized the inhibitory effect of ML on IRF3-mediated promoter activation. Moreover, a recombinant virus expressing a mutant ML protein unable to bind TFIIB was severely impaired in its ability to suppress IFN induction. We concluded that TFIIB binding is required for the IFN antagonist effect exerted by ML. We further demonstrate that the ML-TFIIB interaction has surprisingly little impact on gene expression in general, while a strong negative effect is observed for IRF3- and NF-kappaB-regulated promoters.
authors.affiliation: Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Hermann-Herder-Strasse 11, 79104 Freiburg, Germany.
authors.forename: Carola
authors.initials: C
authors.lastname: Vogt
authors.forename: Ellen
authors.initials: E
authors.lastname: Preuss
authors.forename: Daniel
authors.initials: D
authors.lastname: Mayer
authors.forename: Friedemann
authors.initials: F
authors.lastname: Weber
authors.forename: Martin
authors.initials: M
authors.lastname: Schwemmle
authors.forename: Georg
authors.initials: G
authors.lastname: Kochs