Broad profiling of DNA-binding transcription factor activities improves regulatory network construction in adult mouse tissues.
Molecular systematics involves the description of the regulatory networks formed by the interconnections between active transcription factors and their target expressed genes. Here, we have determined the activities of 200 different transcription factors in six mouse tissues using an advanced mouse oligonucleotide array-based transcription factor assay (MOUSE OATFA). The transcription factor signatures from MOUSE OATFA were combined with public mRNA expression profiles to construct experimental transcriptional regulatory networks in each tissue. SRF-centered regulatory networks constructed for lung and skeletal muscle with OATFA data were confirmed by ChIP assays, and revealed examples of novel networks of expressed genes coregulated by sets of transcription factors. The combination of MOUSE OATFA with bioinformatics analysis of expressed genes provides a new paradigm for the comprehensive prediction of the transcriptional systems and their regulatory pathways in mouse.
Sun
Yi-Min
YM
Medical Systems Biology Research Center, Tsinghua University School of Medicine, Beijing 100084, China.
Zhang
Yan
Y
Zeng
Ling-Qin
LQ
Wu
Jian-Ping
JP
Wei
Li
L
Ren
Ai-Hui
AH
Shao
Wei
W
Qiao
Ji-Ying
JY
Zhao
Yong-Chao
YC
Zhang
Liang
L
Mitchelson
Keith R
KR
Cheng
Jing
J