[Effect of tea polyphenols on proliferation of human periodontal ligament fibroblasts].
OBJECTIVE
To determine the effect of tea polyphenol (TP) on the proliferation of human periodontal ligament fibroblasts (HPDLFs).


METHODS
HPDLFs were primary cultured from tissue explants, and the cells of the 5th to 8th passages were used after immunohistochemical identification (with SABC method) of keratin and vimentin expressions. The cells were divided into 5 groups and treated with TP at 1, 0.5, 0.25, 0.125, and 0.0625 mg/ml, respectively, with another group without TP treatment as the blank control group. Cell counting and MTT colorimetric assay were performed to assess the cell proliferation, and flow cytometry was employed to determine the DNA content of the HPDLFs.


RESULTS
Different concentrations of TP all significantly increased the proliferation and DNA synthesis of the HPDLFs (P<0.05), and TP treatment at 0.5 mg/ml for 6 h produced the optimal effect.


CONCLUSIONS
TP has obviously effect in promoting the proliferation of HPDLFs.
He
Quan-min
QM
Affiliated Stomatological Hospital of Zunyi Medical College, Zunyi, China. hequanmin1972@163.com
Liu
Jian-guo
JG
Xu
Ruo-zhu
RZ
Zhang
Jian
J
Fan
Qin
Q
Jiang
Ce
C