title: Specificity of chicken secondary in vitro lymphoproliferative responses to a parakeet isolate of avian paramyxovirus-3.
abstract: Partially inbred chickens of the Birmingham B2 subline were primed with Paramyxovirus-3/parakeet/Netherlands/449/1975 (PMV 449), and secondary in vitro spleen cell responses were investigated with respect to virus specificity and histocompatibility restriction. PMV 449-primed spleen cells responded to stimulation with syngeneic lymphocytes infected with PMV 449 and other avian paramyxoviruses of the same serogroup, and showed a low level of cross-reaction with Newcastle disease virus. Yucaipa (PMV-2) and Sendai virus were not recognised. Normal Birmingham B2 spleen cells were unresponsive to stimulation with virus-infected lymphocytes. PMV 449-primed spleen cells also responded to stimulation with virus-infected allogeneic lymphocytes under conditions which excluded alloantigen-specific mixed lymphocyte reactions. Possible reasons for the apparent lack of histocompatibility restriction are discussed.
authors.affiliation: Department of Microbiology and Parasitology, Royal Veterinary College, London, England.
authors.forename: M J
authors.initials: MJ
authors.lastname: Cannon
authors.forename: P H
authors.initials: PH
authors.lastname: Russell