title: Identification of 14-3-3zeta by chemical affinity with salicylanilide inhibitors of interleukin-12p40 production.
abstract: Salicylanilides were found as selective inhibitors of interleukin-12p40 production in stimulated dendritic cells. The conversion of one of these bioactive salicylanilides into a comparably bioactive, chemically labeled derivative was achieved using a facile and systematic functional group derivatization strategy. This resulted in a tool reagent that was then employed in an affinity chromatography approach that resulted in the identification of the protein 14-3-3zeta as having selective affinity for the chromatography matrix that was derivatized with a salicylanilide that inhibited IL-12p40 production.
authors.affiliation: Amgen Incorporated, 1201 Amgen Court West, Seattle, Washington 98119Amgen Incorporated, 1201 Amgen Court West, Seattle, Washington 98119, USA. jboyce2000@aol.com
authors.forename: Jim P
authors.initials: JP
authors.lastname: Boyce
authors.forename: Michael E
authors.initials: ME
authors.lastname: Brown
authors.forename: Wilson
authors.initials: W
authors.lastname: Chin
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Fitzner
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Paxton
authors.forename: Min
authors.initials: M
authors.lastname: Shen
authors.forename: Tracey
authors.initials: T
authors.lastname: Stevens
authors.forename: Martin F
authors.initials: MF
authors.lastname: Wolfson
authors.forename: Clifford D
authors.initials: CD
authors.lastname: Wright