title: Time-resolved methods in biophysics. 7. Photon counting vs. analog time-resolved singlet oxygen phosphorescence detection.
abstract: Two recent advances in optoelectronics, namely novel near-IR sensitive photomultipliers and inexpensive yet powerful diode-pumped solid-state lasers working at kHz repetition rate, enable the time-resolved detection of singlet oxygen (O2(a1Deltag)) phosphorescence in photon counting mode, thereby boosting the time-resolution, sensitivity, and dynamic range of this well-established detection technique. Principles underlying this novel approach and selected examples of applications are provided in this perspective, which illustrate the advantages over the conventional analog detection mode.
authors.affiliation: Grup d'Enginyeria Molecular, Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017, Barcelona, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Jiménez-Banzo
authors.forename: Xavier
authors.initials: X
authors.lastname: Ragàs
authors.forename: Peter
authors.initials: P
authors.lastname: Kapusta
authors.forename: Santi
authors.initials: S
authors.lastname: Nonell