Interactome analysis of yeast glutathione peroxidase 3.
Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.
Lee
Phil Young
PY
Translational Research Center, KRIBB, Daejeon 305-806, Korea.
Bae
Kwang-Hee
KH
Kho
Chang Won
CW
Kang
Sunghyun
S
Lee
Do Hee
DH
Cho
Sayeon
S
Kang
Seongman
S
Lee
Sang Chul
SC
Park
Byoung Chul
BC
Park
Sung Goo
SG