title: Oct-2-en-4-ynoyl-CoA as a specific inhibitor of acyl-CoA oxidase.
abstract: Oct-2-en-4-ynoyl-CoA was found to be a specific inhibitor of acyl-CoA oxidase in fatty acid oxidation in peroxisomes that has no inhibitory effect on acyl-CoA dehydrogenase in mitochondria. The inhibition reaction involves a nucleophilic attack of Glu421 to the delta carbon of the inhibitor. The result indicates that acyl-CoA oxidase and acyl-CoA dehydrogenase have certain differences in active-site structure, which makes it possible to control fatty acid oxidation selectively in either mitochondria or peroxisomes with different enzyme inhibitors.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, P. R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zeng
authors.forename: Long
authors.initials: L
authors.lastname: Wu
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Zhang
authors.forename: Yuandong
authors.initials: Y
authors.lastname: Liu
authors.forename: Guisheng
authors.initials: G
authors.lastname: Deng
authors.forename: Ding
authors.initials: D
authors.lastname: Li