title: Vitreous proteomic analysis of proliferative vitreoretinopathy.
abstract: Proliferative vitreoretinopathy (PVR) is the most common cause of anatomic failure in retinal detachment surgery. To understand the molecular mechanisms, vitreous proteomes of patients with PVR were investigated by two-dimensional-nano-liquid chromatography coupled with tandem mass spectrometry. Vitreous samples of moderate PVR (grade B), and severe PVR (grade C or D) were aspirated during pars plana vitrectomy before infusion. In the current study, 129, 97 and 137 proteins were identified in vitreous of normal control, moderate and severe PVR, respectively. In PVR vitreous samples, complement components, serine proteinase inhibitors, and extracellular proteins were up-regulated or appeared, while normal cytoskeleton and metabolism proteins were down-regulated or disappeared. It was noteworthy that the proteins involved in transcription and translation regulation increased in vitreous with PVR. Among 102 PVR-specific proteins, kininogen 1 was specifically detected in both vitreous and the corresponding serum. Therefore, it can be concluded that PVR is a complicated pathology process with great amount of proteins involved in metabolism dysfunction, immune reactions, and cytoskeleton remolding. Kininogen 1 may be a candidate biomarker of PVR. Further investigations of these special proteins will provide additional targets for treatment or prevention of ocular proliferative diseases.
authors.affiliation: The First People Hospital affiliated to Shanghai Jiaotong University, Shanghai, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Shu-Jian
authors.initials: SJ
authors.lastname: Cui
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Song
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.forename: Feng-E
authors.initials: FE
authors.lastname: Chen
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Fang
authors.initials: F
authors.lastname: Wang