title: Fluorine-18-labeled phospholipid quantum dot micelles for in vivo multimodal imaging from whole body to cellular scales.
abstract: We have designed new nanoprobes applicable for both positron emission tomography (PET) and optical fluorescence in vivo imaging. Fluorine-18, which is commonly used for clinical imaging, has been coupled to phospholipid quantum dot (QD) micelles. This probe was injected in mice and we demonstrated that its dynamic quantitative whole body biodistribution and pharmacokinetics could be monitored using PET as well as the kinetics of their cellular uptake using in vivo fibered confocal fluorescence imaging. Phospholipid micelle encapsulation of QDs provides a highly versatile surface chemistry to conjugate multiple chemicals and biomolecules with controlled QD:molecule valency. Here, we show that, in contrast with several previous studies using other QD polymer coatings, these phospholipid QD micelles exhibit long circulation half-time in the bloodstream (on the order of 2 h) and slow uptake by reticulo-endothelial system.
authors.affiliation: CEA, DSV, I(2)BM, SHFJ, Laboratoire d'Imagerie Moleculaire Experimentale, and Inserm U803, 4 place du general Leclerc, 91401 Orsay Cedex, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Ducongé
authors.forename: Thomas
authors.initials: T
authors.lastname: Pons
authors.forename: Carine
authors.initials: C
authors.lastname: Pestourie
authors.forename: Laurence
authors.initials: L
authors.lastname: Hérin
authors.forename: Benoît
authors.initials: B
authors.lastname: Thézé
authors.forename: Karine
authors.initials: K
authors.lastname: Gombert
authors.forename: Benoît
authors.initials: B
authors.lastname: Mahler
authors.forename: Françoise
authors.initials: F
authors.lastname: Hinnen
authors.forename: Bertrand
authors.initials: B
authors.lastname: Kühnast
authors.forename: Frédéric
authors.initials: F
authors.lastname: Dollé
authors.forename: Benoît
authors.initials: B
authors.lastname: Dubertret
authors.forename: Bertrand
authors.initials: B
authors.lastname: Tavitian