title: Repair of sparfloxacin-induced photochemical DNA damage in vivo.
abstract: The induction and subsequent repair of photochemically induced DNA damage by sparfloxacin was assessed in different tissues of juvenile Wistar rats. The animals were treated once orally with 500 mg kg(-1) of sparfloxacin and irradiated 3 hours later with 7 J cm(-2) UVA. Induction and repair of DNA damage was studied in the skin, retina and cornea using the alkaline comet assay. After a tissue-specific increase in the initial DNA damage (higher in the cornea than in skin and retina), an exponential decrease was found in the skin and retina, whereas in cornea a further increase of the DNA damage after 1 hour followed by an exponential decrease was observed. The half-lives for DNA repair were approximately 3 hours for skin and retina and 1 hour for cornea. After a recovery time of 6 hours, the majority of the induced DNA damage detectable with the comet assay had been removed. In conclusion, the data indicate that (1) photochemically induced DNA damage by sparfloxacin is efficiently removed in skin, retina and cornea, (2) repair of these DNA lesions follows an exponential decrease, (3) the induction and repair of sparfloxacin-mediated photochemical DNA damage might be tissue specific.
authors.affiliation: Novartis Pharma AG, Preclinical Safety, Genetic Toxicology and Safety Pharmacology, Basel, Switzerland.
authors.forename: Melanie
authors.initials: M
authors.lastname: Struwe
authors.forename: Karl-Otto
authors.initials: KO
authors.lastname: Greulich
authors.forename: Elias
authors.initials: E
authors.lastname: Perentes
authors.forename: Hans-Jörg
authors.initials: HJ
authors.lastname: Martus
authors.forename: Willi
authors.initials: W
authors.lastname: Suter
authors.forename: Ulla
authors.initials: U
authors.lastname: Plappert-Helbig