title: Change in proteomic profiles of genetically modified 1,3-propanediol-producing recombinant E. coli.
abstract: The recombinant E. coli Delta6 mutant (galR, glpK, gldA, IdhA, lacI, tpiA) was used to produce 1,3-propanediol (PD) from glucose. The 1,3-PD production increased with feedback control of the glucose concentration using fed-batch fermentation. The maximum 1,3-PD concentration produced was 43 g/l after 60 h of fermentation. Glycerol production was minimized when controlling the glucose concentration at less than 1 g/l. The expression levels of seven enzymes related to the 1,3-PD production metabolism were compared during the cell growth phase and 1,3-PD production phase, and their expression levels all increased during 1,3-PD production, with the exception of alcohol dehydrogenase.
authors.affiliation: Department of Chemical Engineering, Chosun University, Gwangju, Korea.
authors.forename: Li Hua
authors.initials: LH
authors.lastname: Jin
authors.forename: Jung-Heon
authors.initials: JH
authors.lastname: Lee