Production of virus-specific antisera to lymphoproliferative disease virus of turkeys.
Lymphoproliferative disease virus, a recently isolated C-type retro-virus, cannot yet be cultured in cells in vitro and rarely induces a high titre virus-specific antiserum. However, after digestion of purified virus with bromelain and rebanding the treated virus in sucrose gradients bald virions devoid of the viral spike glycoprotein and up to 20 host proteins were found to induce a high level virus-specific antibody response in rabbits and chickens.
Patel
J R
JR
Houghton Poultry Research Station, Houghton, Huntingdon, Cambridgeshire, England.
Shilleto
R W
RW