title: MIFlowCyt: the minimum information about a Flow Cytometry Experiment.
abstract: A fundamental tenet of scientific research is that published results are open to independent validation and refutation. Minimum data standards aid data providers, users, and publishers by providing a specification of what is required to unambiguously interpret experimental findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a cross-disciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the FCM experiment, the configuration of the instrument used to perform the assays, and the data processing approaches used to interpret the primary output data. MIFlowCyt has been adopted as a standard by ISAC, representing the FCM scientific community including scientists as well as software and hardware manufacturers. Adoptionof MIFlowCyt by the scientific and publishing communities will facilitate third-party understanding and reuse of FCM data.
authors.affiliation: Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
authors.forename: Jamie A
authors.initials: JA
authors.lastname: Lee
authors.forename: Josef
authors.initials: J
authors.lastname: Spidlen
authors.forename: Keith
authors.initials: K
authors.lastname: Boyce
authors.forename: Jennifer
authors.initials: J
authors.lastname: Cai
authors.forename: Nicholas
authors.initials: N
authors.lastname: Crosbie
authors.forename: Mark
authors.initials: M
authors.lastname: Dalphin
authors.forename: Jeff
authors.initials: J
authors.lastname: Furlong
authors.forename: Maura
authors.initials: M
authors.lastname: Gasparetto
authors.forename: Michael
authors.initials: M
authors.lastname: Goldberg
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Goralczyk
authors.forename: Bill
authors.initials: B
authors.lastname: Hyun
authors.forename: Kirstin
authors.initials: K
authors.lastname: Jansen
authors.forename: Tobias
authors.initials: T
authors.lastname: Kollmann
authors.forename: Megan
authors.initials: M
authors.lastname: Kong
authors.forename: Robert
authors.initials: R
authors.lastname: Leif
authors.forename: Shannon
authors.initials: S
authors.lastname: McWeeney
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Moloshok
authors.forename: Wayne
authors.initials: W
authors.lastname: Moore
authors.forename: Garry
authors.initials: G
authors.lastname: Nolan
authors.forename: John
authors.initials: J
authors.lastname: Nolan
authors.forename: Janko
authors.initials: J
authors.lastname: Nikolich-Zugich
authors.forename: David
authors.initials: D
authors.lastname: Parrish
authors.forename: Barclay
authors.initials: B
authors.lastname: Purcell
authors.forename: Yu
authors.initials: Y
authors.lastname: Qian
authors.forename: Biruntha
authors.initials: B
authors.lastname: Selvaraj
authors.forename: Clayton
authors.initials: C
authors.lastname: Smith
authors.forename: Olga
authors.initials: O
authors.lastname: Tchuvatkina
authors.forename: Anne
authors.initials: A
authors.lastname: Wertheimer
authors.forename: Peter
authors.initials: P
authors.lastname: Wilkinson
authors.forename: Christopher
authors.initials: C
authors.lastname: Wilson
authors.forename: James
authors.initials: J
authors.lastname: Wood
authors.forename: Robert
authors.initials: R
authors.lastname: Zigon
authors.forename: Richard H
authors.initials: RH
authors.lastname: Scheuermann
authors.forename: Ryan R
authors.initials: RR
authors.lastname: Brinkman