title: [Association of single nucleotide polymorphism at interleukin-10 gene 1082 nt with the risk of gastric cancer in Chinese population].
abstract: OBJECTIVE
  To study the association of single nucleotide polymorphism at interleukin-10 gene 1082 locus with Helicobacter pylori (Hp) infection and the risk of gastric cancer in high prevalent region (Shaanxi Province)aand low prevalence region (Guangdong Province) in China.
  
  
  METHODS
  The genomic DNA was extracted from the peripheral blood of 104 healthy individuals, 104 gastric cancer patients from Guangdong Province, and from 102 healthy volunteers and 102 gastric cancer patients in Shaanxi Province, China. The single nucleotide polymorphism at IL-10 gene 1082 locus was analyzed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The serum levels of anit-Hp IgG was measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The frequencies of IL-10-1082 A/A, A/G and G/G genotypes in the 412 subjects were 86.7%, 10.7% and 2.4%, respectively. In the low prevalence region, the number of carriers of IL-10-1082 G* was much greater in the cancer patients than in the healthy controls (14.4% vs 7.7%, Chi2=4.02, P<0.05, OR=1.01, 95% CI=1.08-3.10). The presence of IL-10-1082 G* was associated with significantly increased risk of gastric cancer following Hp infection (Chi(2)=5.36, P<0.05, OR=6.0, 95% CI=1.23-17.52). In the high prevalence region, the frequency of IL-10-1082 G* was slightly higher among the cancer patients than in the healthy controls, but this difference was not statistically significant (12.7% vs 16.6%, P>0.05).
  
  
  CONCLUSIONS
  The G* genotype of IL-10 gene 1082 locus may be associated with increased risk of gastric cancer in China.
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China. zhousz@gmail.com
authors.forename: Shao-zhang
authors.initials: SZ
authors.lastname: Zhou
authors.forename: Wei-liang
authors.initials: WL
authors.lastname: Zhu
authors.forename: Ming-ying
authors.initials: MY
authors.lastname: Li
authors.forename: Hong-yi
authors.initials: HY
authors.lastname: Li
authors.forename: Ji-ren
authors.initials: JR
authors.lastname: Zhang