title: Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases.
abstract: Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.
authors.affiliation: Jiangsu Diabetes Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Ba
authors.forename: Lijia
authors.initials: L
authors.lastname: Ma
authors.forename: Xing
authors.initials: X
authors.lastname: Cai
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yin
authors.forename: Kehui
authors.initials: K
authors.lastname: Wang
authors.forename: Jigang
authors.initials: J
authors.lastname: Guo
authors.forename: Yujing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jiangning
authors.initials: J
authors.lastname: Chen
authors.forename: Xing
authors.initials: X
authors.lastname: Guo
authors.forename: Qibin
authors.initials: Q
authors.lastname: Li
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Li
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Jiang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.forename: Pingping
authors.initials: P
authors.lastname: Zheng
authors.forename: Juanbin
authors.initials: J
authors.lastname: Zhang
authors.forename: Ruiqiang
authors.initials: R
authors.lastname: Li
authors.forename: Hongjie
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Shang
authors.forename: Ting
authors.initials: T
authors.lastname: Gong
authors.forename: Guang
authors.initials: G
authors.lastname: Ning
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Zen
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Zhang