title: [Effect of compound Puerarin on the collage IV in streptozotocin-induced diabetic nephropathy rats].
abstract: OBJECTIVE
  To observe the effect of compound Puerarin on collagen IV of streptozotocin-induced diabetic rats.
  
  
  METHODS
  Diabetic nephropathy rats were induced by intraperitoneal injection of streptozotocin (STZ). Rats were allocated randomly to control group (10), diabetes model group (10), Vitamin C group (10), Puerarin group (10), vitamin C plus Puerarin group (10). The study period lasted for 12 weeks. During and after the treatment, the general state, blood glucose levels, glycosylated hemoglobin, blood urea nitrogen, serum collagen IV, blood urea nitrogen, serum creatinine, urinary albumin excretion rate of the 24-hour, and clearance rate of creatinine collagen IV protein were determined by immunohistochemistoche analysis as well as type the gene expression of collagen IV alpha 1 mRNA were determined by in situ hybridization analysis in the kidney tissue of different groups.
  
  
  RESULTS
  (1) Diabetes mellitus and renal function lesion occurred in the four groups. (2) Vitamin C and Puerarin could improve the general conditions of diabetic Rats, decrease blood urea nitrogen [(8.68 +/- 0.43), (7.98 +/- 0.47) and (5.76 +/- 0.82) micromol/L, serum creatinine [(74.68 +/- 8.20), (75.52 +/- 7.98) and (58.66 +/- 6.65) mmol/L], and urinary albumin excretion rate of the 24-hour [(18.40 +/- 0.37), (17.24 +/- 0.30) and (9.97 +/- 1.27) mg/24 h x 10(-3)]; increase clearance rate of creatinine [(0.59 +/- 0.21), (0.61 +/- 0.14) and (0.69 +/- 0.32) ml/min], the expression of collage IV absorbance [(111.56 +/- 14.61), (110.78 +/- 9.69) and (95.44 +/- 9.97) ] in the diabetic Rats were significantly inhibited at the same time.
  
  
  CONCLUSIONS
  The compound Puerarin might have some functions on preventing ren by inhibiting expression of type IV collagen.
authors.affiliation: Department of Endocrinology, Loudi Municipal Central Hospital of Hunan Province, Loudi 417000, China.
authors.forename: Qiang-xiang
authors.initials: QX
authors.lastname: Li
authors.forename: Hui-ju
authors.initials: HJ
authors.lastname: Zhong
authors.forename: Han-ren
authors.initials: HR
authors.lastname: Gong
authors.forename: Fei-yue
authors.initials: FY
authors.lastname: Zhu
authors.forename: Lin-na
authors.initials: LN
authors.lastname: Wang
authors.forename: Dao-jun
authors.initials: DJ
authors.lastname: Shen
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.forename: Cai-yun
authors.initials: CY
authors.lastname: Wang
authors.forename: Cheng-sheng
authors.initials: CS
authors.lastname: Qin