title: Enhanced c-Fms/M-CSF receptor signaling and wound-healing process in bone marrow-derived macrophages of signal-transducing adaptor protein-2 (STAP-2) deficient mice.
abstract: Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein as a c-Fms/M-CSF receptor-interacting protein and constitutively expressed in macrophages. In our previous study, we examined the role of STAP-2 in the c-Fms/M-CSF receptor signaling using a murine macrophage tumor cells line, Raw264.7. Overexpression of STAP-2 in Raw264.7 cells markedly suppressed M-CSF-induced activation of extracellular signal regulated kinase and Akt. In addition, Raw264.7 overexpressing STAP-2 affected cell migration in wound-healing process. These results suggest that STAP-2 deficiency influences endogenous c-Fms/M-CSF receptor signaling. Here we show that loss of STAP-2 expression in knockout mouse macrophages results in marked enhancement of the c-Fms/M-CSF receptor signaling and wound-healing process. We therefore propose that STAP-2 acts as an endogenous regulator in normal macrophages functions.
authors.affiliation: Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku Kita 12 Nishi 6, Sapporo, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Ikeda
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sekine
authors.forename: Ryuta
authors.initials: R
authors.lastname: Muromoto
authors.forename: Norihiko
authors.initials: N
authors.lastname: Ohbayashi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yoshimura
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsuda