Direct and quantitative analysis of Salmonella enterica serovar Typhimurium using real-time PCR from artificially contaminated chicken meat.
For quantitative PCR assay of Salmonella enterica serovar Typhimurium in food samples, a real-time PCR method was developed, based on DNA genome equivalent. Specific primers and probe designed based on the STM4497 gene of S. Typhimurium LT2 showed the specificity to S. Typhimurium. Threshold cycle (Ct) values of real-time PCR were obtained from a quantitative standard curve with genomic DNA of Salmonella Typhimurium. In addition, the recovery of S. Typhimurium inoculated artificially to chicken samples with 4.5 x 10(5) to 4.5 CFU/ml was evaluated by using real-time PCR and plate-count methods. Result showed that the number of cells calculated from the real-time PCR method had good correlation with that of the plate-count method. This real-time PCR method could be applicable to the detection and quantification of S. Typhimurium in food samples.
Park
Hee-Jin
HJ
Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea.
Kim
Hyun-Joong
HJ
Park
Si-Hong
SH
Shin
Eun-Gyeong
EG
Kim
Jae-Hwan
JH
Kim
Hae-Yeong
HY