title: An infectious cDNA clone of a highly pathogenic porcine reproductive and respiratory syndrome virus variant associated with porcine high fever syndrome.
abstract: Since May 2006, a so-called 'porcine high fever syndrome' (PHFS) has spread all over China. The arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) was believed to be the main causative agent, although the involvement of other pathogens was not formally excluded. The genome of a representative Chinese PRRSV strain, named JX143, was sequenced and used to develop infectious cDNA clones, pJX143 and pJX143M, with the latter containing an engineered MluI site that served as a genetic marker. In various virological assays, the rescued viruses, vJX143 and vJX143M, were indistinguishable from their parental virus. Animal experiments showed that these recombinant viruses retained the high pathogenicity and induced the typical clinical symptoms observed during PHFS outbreaks. This is the first report describing infectious cDNA clones of this highly pathogenic PRRSV. Our results unambiguously fulfil Koch's postulates and define highly pathogenic PRRSV as the aetiological agent of PHFS in China.
authors.affiliation: Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, The Key Laboratory of Animal Parasitology, Chinese Ministry of Agriculture, Shanghai 200232, PR China.|China Agricultural University, College of Biological Sciences, Beijing 100094, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lv
authors.affiliation: Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, The Key Laboratory of Animal Parasitology, Chinese Ministry of Agriculture, Shanghai 200232, PR China.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, The Key Laboratory of Animal Parasitology, Chinese Ministry of Agriculture, Shanghai 200232, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Sun
authors.affiliation: China Agricultural University, College of Biological Sciences, Beijing 100094, PR China.
authors.forename: Weiquan
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, The Key Laboratory of Animal Parasitology, Chinese Ministry of Agriculture, Shanghai 200232, PR China.
authors.forename: Shishan
authors.initials: S
authors.lastname: Yuan