title: Loss of homologous recombination or non-homologous end-joining leads to radial formation following DNA interstrand crosslink damage.
abstract: High levels of interstrand cross-link damage in mammalian cells cause chromatid breaks and radial formations recognizable by cytogenetic examination. The mechanism of radial formation observed following DNA damage has yet to be determined. Due to recent findings linking homologous recombination and non-homologous end-joining to the action of the Fanconi anemia pathway, we speculated that radials might be the result of defects in either of the pathways of DNA repair. To test this hypothesis, we have investigated the role of homologous recombination proteins RAD51 and RAD52, non-homologous end-joining proteins Ku70 and LIG4, and protein MRE11 in radial formation and cell survival following interstrand crosslink damage with mitomycin C. For the studies we used small inhibitory RNA to deplete the proteins from cells, allowing for evaluation of radial formation and cell survival. In transformed normal human fibroblasts, depletion of these proteins increased interstrand crosslink sensitivity as manifested by decreased cell survival and increased radial formation. These results demonstrate that inactivation of proteins from either of the two separate DNA repair pathways increases cellular sensitivity to interstrand crosslinks, indicating each pathway plays a role in the normal response to interstrand crosslink damage. We can also conclude that homologous recombination or non-homologous end-joining are not required for radial formation, since radials occur with depletion of these pathways.
authors.affiliation: Department of Molecular and Medical Genetics, Oregon Health & Science University, Portland, OR 97239, USA.
authors.forename: A E
authors.initials: AE
authors.lastname: Hanlon Newell
authors.forename: A
authors.initials: A
authors.lastname: Hemphill
authors.forename: Y M N
authors.initials: YM
authors.lastname: Akkari
authors.forename: J
authors.initials: J
authors.lastname: Hejna
authors.forename: R E
authors.initials: RE
authors.lastname: Moses
authors.forename: S B
authors.initials: SB
authors.lastname: Olson