The Population Reference Sample, POPRES: a resource for population, disease, and pharmacological genetics research.
Technological and scientific advances, stemming in large part from the Human Genome and HapMap projects, have made large-scale, genome-wide investigations feasible and cost effective. These advances have the potential to dramatically impact drug discovery and development by identifying genetic factors that contribute to variation in disease risk as well as drug pharmacokinetics, treatment efficacy, and adverse drug reactions. In spite of the technological advancements, successful application in biomedical research would be limited without access to suitable sample collections. To facilitate exploratory genetics research, we have assembled a DNA resource from a large number of subjects participating in multiple studies throughout the world. This growing resource was initially genotyped with a commercially available genome-wide 500,000 single-nucleotide polymorphism panel. This project includes nearly 6,000 subjects of African-American, East Asian, South Asian, Mexican, and European origin. Seven informative axes of variation identified via principal-component analysis (PCA) of these data confirm the overall integrity of the data and highlight important features of the genetic structure of diverse populations. The potential value of such extensively genotyped collections is illustrated by selection of genetically matched population controls in a genome-wide analysis of abacavir-associated hypersensitivity reaction. We find that matching based on country of origin, identity-by-state distance, and multidimensional PCA do similarly well to control the type I error rate. The genotype and demographic data from this reference sample are freely available through the NCBI database of Genotypes and Phenotypes (dbGaP).
Nelson
Matthew R
MR
GlaxoSmithKline, Research Triangle Park, NC 27709, USA. matthew.r.nelson@gsk.com
Bryc
Katarzyna
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King
Karen S
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Indap
Amit
A
Boyko
Adam R
AR
Novembre
John
J
Briley
Linda P
LP
Maruyama
Yuka
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Waterworth
Dawn M
DM
Waeber
Gérard
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Vollenweider
Peter
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Oksenberg
Jorge R
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Hauser
Stephen L
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Stirnadel
Heide A
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Kooner
Jaspal S
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Chambers
John C
JC
Jones
Brendan
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Mooser
Vincent
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Bustamante
Carlos D
CD
Roses
Allen D
AD
Burns
Daniel K
DK
Ehm
Margaret G
MG
Lai
Eric H
EH