title: Scutellarin isolated from Erigeron multiradiatus inhibits high glucose-mediated vascular inflammation.
abstract: Erigeron multiradiatus (Lindl.) Benth is a traditional Tibetan medicine herb long used to treat various diseases related to inflammation. Our previous phytochemical studies on E. multiradiatus resulted in the isolation of scutellarin, which is a known flavone glucuronide with comprehensive pharmacological actions. In present study, we investigated the inhibition action of scutellarin on high glucose-induced vascular inflammation in human endothelial cells (ECV304 cells). Consistent with previous reports, exposure of ECV304 cells to high glucose for 24 h caused an increase of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein 1 (MCP-1), and promoted cell adhesion between monocyte and ECV304 cells. However, pretreatment with scutellarin (0.1 and 1 microM) reversed these effects in a concentration-dependent manner. Scutellarin was able to inhibit the activation of NF-kappaB induced by high glucose in ECV304 cells. Furthermore, although oral administration of scutellarin (10 and 50 mg/kg) did not produce significant antihyperglycemic action, it lowered the serum MCP-1 levels significantly in alloxan-induced diabetic mice. Therefore, our results suggest that scutellarin has anti-inflammation effect that may afford some protection against hyperglycemia-induced vascular inflammatory both in vitro and in vivo.
authors.affiliation: West China School of Pharmacy, Sichuan University, No. 17 Section 3 Ren-Min-Nan-Lu Road, Chengdu, Sichuan, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Luo
authors.forename: Zheng-Huai
authors.initials: ZH
authors.lastname: Tan
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Xian-Fu
authors.initials: XF
authors.lastname: Liu
authors.forename: Zheng-Ji
authors.initials: ZJ
authors.lastname: Mo