title: Nocardia altamirensis sp. nov., isolated from Altamira cave, Cantabria, Spain.
abstract: A novel actinomycete strain, OFN S17(T), was isolated from a sample collected from Altamira Cave, Cantabria, Spain. This strain was identified by using a polyphasic taxonomic approach. The 16S rRNA, hsp65 and sod gene sequences of the strain were determined and compared with those of representative Nocardia species. The results showed that strain OFN S17(T) should be assigned to the genus Nocardia. Phylogenetic analysis indicated that strain OFN S17(T) was most closely related to the type strain of Nocardia tenerifensis (98.6, 96.2 and 96% similarity, respectively, for the 16S rRNA, hsp65 and sod gene sequences). The DNA G+C content was 64.4 mol%. DNA-DNA hybridization analyses revealed 29% relative reassociation between the DNA of strain OFN S17(T) and N. tenerifensis DSM 44704(T). The phenotypic and genotypic data show that strain OFN S17(T) merits recognition as a representative of a novel species of the genus Nocardia, for which the name Nocardia altamirensis sp. nov. is proposed. The type strain is OFN S17(T) (=CIP 109606(T) =DSM 44997(T)).
authors.affiliation: Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, E-41080 Sevilla, Spain.
authors.forename: Valme
authors.initials: V
authors.lastname: Jurado
authors.forename: Patrick
authors.initials: P
authors.lastname: Boiron
authors.forename: Reiner M
authors.initials: RM
authors.lastname: Kroppenstedt
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laurent
authors.forename: Andrée
authors.initials: A
authors.lastname: Couble
authors.forename: Leonila
authors.initials: L
authors.lastname: Laiz
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.forename: Juan M
authors.initials: JM
authors.lastname: González
authors.forename: Cesareo
authors.initials: C
authors.lastname: Saiz-Jimenez
authors.forename: Delphine
authors.initials: D
authors.lastname: Mouniée
authors.forename: Emanuelle
authors.initials: E
authors.lastname: Bergeron
authors.forename: Verónica
authors.initials: V
authors.lastname: Rodríguez-Nava