An enzyme-linked immunosorbent assay (ELISA) for the detection of chlamydial antibody in duck sera.
An enzyme-linked immunosorbent assay (ELISA) for the detection of chlamydial antibody in duck sera is described. The assay was used to test sera from a flock of domestic Pekin ducks infected with Chlamydia psittaci, and sera from wildfowl (Mallard). Sera from 63% of the domestic birds and 75% of the wildfowl exhibited chlamydial antibody titres of >/= 1/32, indicative of current or past chlamydial infection. There was a good correlation between the results obtained with the ELISA and those obtained by a micro-immunofluorescence test. The application of the ELISA to large scale screening of flocks is suggested.
Evans
R T
RT
Sexually Transmitted Diseases Research Group, Division of Communicable Diseases, MRC Clinical Research Centre, Harrow, Middlesex, England.
Chalmers
W S
WS
Woolcock
P R
PR
Farmer
H
H
Taylor-Robinson
D
D