[Molecular characterization of Chilean isolates of streptococcus agalactiae].
BACKGROUND
Streptococcus agalactiae is the main causing organism of invasive infections such as sepsis and meningitis in the newborn.


OBJECTIVE
To perform a genotype characterization of Streptococcus agalactiae strains coming form invasive infections of newborns and colonized pregnant women.


METHODS
A group of 58 strains not related epidemiologically isolated from colonized pregnant women and invasive infections in newborns, were studied. Pulsed field electrophoresis (PFGE) and polymerase chain reaction amplification of hylB and IS 1,548 genes, as possible virulence markers, were performed.


RESULTS
Among the studied strains, 37 genetic subtypes were observed. There were nine groups of identical PFGE patterns. Three corresponded to serotype la and six to serotype III. An erythromycin and clindamycin resistant clone was identified in three colonized women and a newborn with sepsis, which were not epidemiologically related. The hylB gene was equally present in cases of neonatal meningitis or colonized pregnant women.


CONCLUSIONS
There was a great degree of polymorphism among the studied strains. The ample presence of hylB gene and the absence of the insertion element IS1548 in the hylB gene in invasive and colonizing strains, indicates that both groups of strains are potentially pathogenic.
Rojo
Patricia
P
Sección Bacteriología Clínica, Instituto de Salud Pública de Chile, Chile.
Araya
Pamela
P
Martínez T
M Angélica
MA
Hormazábal
Juan Carlos
JC
Maldonado
Aurora
A
Fernández
Jorge
J