Structural and functional studies indicate that Shigella VirA is not a protease and does not directly destabilize microtubules.
VirA, an essential virulence factor in Shigella disease pathogenesis, is involved in the uptake, motility, and cell-to-cell spread of Shigella organisms within the human host. These functions have been attributed to a VirA protease activity and a mechanism of microtubule destruction via tubulin degradation [Yoshida, S., et al. (2006) Science 314, 985-989]. We report functional and crystallographic data indicating a novel VirA structure that lacks these activities but highlights the homology to the EspG virulence factor of pathogenic Escherichia coli.
Germane
Katherine L
KL
Department of Pharmacology, Vanderbilt University Medical Center, 461 Preston Research Building, 2220 Pierce Avenue, Nashville, Tennessee 37232, USA.
Ohi
Ryoma
R
Goldberg
Marcia B
MB
Spiller
Benjamin W
BW