title: Cell-mediated immune response to unrelated proteins and unspecific inflammation blocked by orally tolerated proteins.
abstract: Oral tolerance promotes a generalized decrease in specific immune responsiveness to proteins previously encountered via the oral route. In addition, parenteral immunization with a tolerated protein also triggers a significant reduction in the primary responsiveness to a second unrelated antigen. This is generally explained by 'innocent bystander suppression', suggesting that the transient and episodic effects of inhibitory cytokines released by contact with the tolerated antigen would block responses to the second antigen. In disagreement with this view, we have previously shown that: (i) these inhibitory effects do not require concomitance or contiguity of the injections of the two proteins; (ii) that intravenous or intragastric exposures to the tolerated antigen are not inhibitory; and (iii) that the inhibitory effect, once triggered, persists in the absence of further contact with the tolerated protein, possibly by inhibition of secondary responsiveness (immunological memory). The present work confirms that immunological memory of the second unrelated antigen is hindered by exposure to the tolerated antigen and, in addition, shows that this exposure: (i) inhibits the inflammation triggered by an unrelated antigen through the double effect of inhibiting production of leucocytes in the bone marrow and blocking their migration to inflammed sites; and (ii) significantly blocks footpaw swelling triggered by carrageenan. Taken together, these results conclusively demonstrate that inhibitory effects of parenteral injection of tolerated antigens are much more general than suggested by the 'innocent bystander suppression' hypothesis.
authors.affiliation: Department of Pharmacology, CCB, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: Gustavo C
authors.initials: GC
authors.lastname: Ramos
authors.forename: Claudiney M
authors.initials: CM
authors.lastname: Rodrigues
authors.forename: Geraldo M
authors.initials: GM
authors.lastname: Azevedo
authors.forename: Vanessa
authors.initials: V
authors.lastname: Pinho
authors.forename: Cláudia R
authors.initials: CR
authors.lastname: Carvalho
authors.forename: Nelson M
authors.initials: NM
authors.lastname: Vaz