The UL49 gene product of BoHV-1: a major factor in efficient cell-to-cell spread.
The role of the UL49 gene product, VP22, of bovine herpesvirus type 1 (BoHV-1) in virus replication was characterized with respect to a putative functional interaction of VP22 with the viral glycoprotein E (gE) during BoHV-1 cell-to-cell spread. Deletion of the open reading frames of UL49 and/or gE from an infectious BoHV-1 bacterial artificial chromosome clone did not severely impair the production of viral progeny in single-step growth experiments. However, plaque sizes induced by a VP22-negative BoHV-1 were reduced by 52%, whilst for the gE/VP22-negative double-deletion mutant a reduction of 83% could be observed in comparison with parental and revertant viruses, which was consistent with a marked reduction in multi-step growth experiments at early time points. These results suggest that gE and VP22 are important for BoHV-1 cell-to-cell spread, and that both are likely to act independently of each other in a critical pathway for virus cell-to-cell spread.
Kalthoff
Donata
D
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald, Germany.
Granzow
Harald
H
Institute of Infectology, Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald, Germany.
Trapp
Sascha
S
Institute for Medical Microbiology, Infectious and Epidemic Diseases, Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Germany.
Beer
Martin
M
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald, Germany.