title: Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep.
abstract: The early distribution of Brucella melitensis and the immune response induced in lymphoid tissues and lymph nodes (LN) draining the upper respiratory tract were analysed in sheep. An experimental acute infection was performed by inoculating the sheep with the virulent H38 strain of B. melitensis by the conjunctival route. The infection was rapidly controlled at the site of inoculation but resulted in a local and systemic dissemination of brucellae mainly in the pharyngeal tonsil, local and peripheral LN and the spleen. The control of the infection was associated with the induction of a specific immune response characterized by an increase in IgG+ cells, the production of IFN-gamma and IL-10 by cells from draining parotid, retropharyngeal and submaxillary LN, but also from more distant peripheral prescapular and mesenteric LN. IFN-gamma was produced by CD4+, CD8+ and CD4(-)CD8(-)gammadelta(-) cells and probably contributed to the control of both local and systemic infection.
authors.affiliation: UR 1282 - Unité d'Infectiologie Animale et Santé Publique, Institut National de Recherche Agronomique, Centre de Tours-Nouzilly, 37380 Nouzilly, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Suraud
authors.forename: Isabelle
authors.initials: I
authors.lastname: Jacques
authors.forename: Michel
authors.initials: M
authors.lastname: Olivier
authors.forename: Laurence A
authors.initials: LA
authors.lastname: Guilloteau