title: Biodesulfurization of dibenzothiophene by growing cells of Pseudomonas putida CECT 5279 in biphasic media.
abstract: Several studies have proven that natural or genetically modified bacteria, such as Pseudomonas putida strain, degrade recalcitrant organic sulfur compounds. However, from a practical point of view, the biodesulfurization (BDS) process has to be performed with really high proportions of organic solvents. In this work, the dibenzothiophene (DBT) was selected as recalcitrant model compound, and hexadecane as model organic solvent. It has been observed that P. putida CECT 5279 was able to desulfurize DBT even in the presence of 50% (v/v) of hexadecane. A concentration of 400 ppm of DBT was converted at a specific rate of generation of desulfurized final product, 2-hydroxybiphenyl (HBP), of 2.3 and 1.5 mg HBP L-1 (g DC L-1 h)-1 for 27% and 50% (v/v) of hexadecane, respectively. Finally, the Haldane kinetic model was used to describe the process evolution. The study is relevant as it has been proven that the strain CECT 5279 is a potential biocatalyst for developing an efficient BDS process.
authors.affiliation: Dpto. Quimica Analitica e Ingenieria Quimica, Facultad de Ciencias, Universidad de Alcala, Madrid, Spain.
authors.forename: Ainhoa
authors.initials: A
authors.lastname: Caro
authors.forename: Karina
authors.initials: K
authors.lastname: Boltes
authors.forename: Pero
authors.initials: P
authors.lastname: Leton
authors.forename: Eloy
authors.initials: E
authors.lastname: Garcia-Calvo