Click chemistry for high-density biofunctionalization of mesoporous silica.
The applicability of click chemistry for high-density functionalization of mesoporous silica is demonstrated. The mild conditions of the copper(I)-catalyzed Huisgen reaction allow for a surface functionalization with intact biomolecules. The high covalent enzyme functionalization density under simultaneous retention of enzyme activity and the absence of leaching demonstrate the promising potential of this approach.
Schlossbauer
Axel
A
Department of Chemistry and Biochemistry, Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstr. 5-13 (E), 81377 Munich, Germany.
Schaffert
David
D
Kecht
Johann
J
Wagner
Ernst
E
Bein
Thomas
T