Microdissection of additional chromosome in common wheat-Th. intermedium TAI-27 and screening of its special probe.
It was identified that there were 4 St chromosomes derived from Th. intermedium in common wheat-Th. intermedium alien additional line TAI-27 by in situ hybridization. Two St chromosomes added to wheat chromosome in TAI-27 as well as two of them replaced two of 42 in wheat chromosomes. This indicates that TAI-27 is not only an alien additional line, but also a replacing line. The additional chromosomes in TAI-27 were microdissected and a microcloning library was constructed. A special probe of Th. intermedium was obtained from a microcloning library. The sequence analysis indicated that there were no homology with Genebank data. This makes it possible to screen genes with the disease-resistance, adversity-tolerance and fine character from Th.intermedium.
Tian
C
C
Institute of Plant Science and Technology, Chinese Agricultural University, Beijing, China.
Lu
Y
Y
Deng
J
J
Li
B
B
Zhang
X
X
Liu
G
G