Heterologous expression ofa histone-like protein from Streptococcus intermedius in Escherichia coli alters the nucleoid structure and inhibits the growth of E. coli.
Escherichia coli failed to survive after transformation with a Streptococcus intermedius histone-like protein gene (Si-hlp) and its promoter-harbored plasmid. The promoter function of Si-hlp in E. coli was determined using enhanced green fluorescence protein (egfp) gene as a reporter. The inhibitory effect of Si-HLP on E. coli viability was verified by a tetracycline-inducible gene expression system. Further study suggested that Si-HLP may alter the bacterial nucleoid structure, leading to the growth inhibition of E. coli.
Liu
Dali
D
Yumoto
Hiromichi
H
Murakami
Keiji
K
Hirota
Katsuhiko
K
Kayama
Shizuo
S
Taniguchi
Tomonori
T
Yamamoto
Akitake
A
Ono
Tsuneko
T
Matsuo
Takashi
T
Miyake
Yoichiro
Y