title: Cryopreservation of sugarcane somatic embryos.
abstract: In this paper, we compared three vitrification-based cryopreservation techniques, viz. vitrification, encapsulation-vitrification and droplet-vitrification for cryopreserving sugarcane somatic embryos. Viability of somatic embryos was evaluated by measuring electrolyte leakage and by regrowth on recovery medium. Droplet-vitrification was the most efficient technique. Optimal conditions included loading with a solution containing 1.5 M glycerol and 0.3 M sucrose for 30 min at 25 degree C, treatment with the PVS2 solution for 20-40 min at 0 degree C followed by rapid immersion in liquid nitrogen of clumps of somatic embryos placed in microdroplets of cryoprotectant solution. Under such conditions, viability of cryopreserved somatic embryos reached 55 percent.
authors.affiliation: University of Ciego de Avila, Bioplantas Centre, Ciego de Avila, Cuba. marcosem@bioplantas.cu
authors.forename: Marcos
authors.initials: M
authors.lastname: Martinez-Montero
authors.forename: Julia
authors.initials: J
authors.lastname: Martinez
authors.forename: Florent
authors.initials: F
authors.lastname: Engelmann