title: Autophagy is activated, but is not required for the G0 function of BCL-2 or BCL-xL.
abstract: Cell cycle arrest in G(0) and autophagy have features in common, but the inter-relationship between the two processes is not well defined. The anti-apoptosis molecules BCL-2 and BCL-x(L) promote G(0) arrest through upregulation of p27 protein, which can also induce autophagy. We tested the hypothesis that autophagy was involved in the cell cycle arrest function of BCL-2 and BCL-x(L). We found that in IL-3-dependent FL5.12 cells, NIH3T3 cells and mouse embryo fibroblasts induced to arrest, treatment with 3-methyladenine did not affect the expected decrease in cell size and ribosomal RNA synthesis, or upregulation of p27 levels. Using the m5-7 ATG5(-/-) MEF cell line with doxycycline-regulated ATG5 expression, we demonstrated that autophagy was activated during serum withdrawal and contact inhibition, but inhibition of autophagy had no measurable effect on G(0) arrest in parental or BCL-x(L)-expressing cells. Thus, our data indicate that, in cell culture models, autophagy occurs but is not required for entrance into quiescence or for the G(0) function of BCL-2 or BCL-x(L).
authors.affiliation: Departments of Cancer Biology and Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
authors.forename: Mayda
authors.initials: M
authors.lastname: Valentin
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Yang