title: Molecular characterization and expression pattern of fetuin-B in gibel carp (Carassius auratus gibelio).
abstract: Fetuin-B has recently been cloned and identified from rats, mice, and humans; their expression patterns, however, have not been elucidated yet. In this study, Cagfetuin-B has been cloned in gibel carp. RT-PCR and Western blot detection revealed that Cagfetuin-B is first transcribed from the blastula stage and at a relatively stable level afterward during embryogenesis and the larval stage. Cagfetuin-B transcripts are predominantly distributed over the yolk syncytial layer in the early embryos and later restricted to the cells of liver and brain in newly hatched larvae. Moreover, a dynamic distribution of Cagfetuin-B protein was observed in brain, kidney, liver, and skin during morphogenesis. In adult fish, Cagfetuin-B transcripts are restricted in liver and ovary. Our work, for the first time, revealed the extra-hepatic transcription and a dynamic distribution of fetuin-B during embryogenesis and in adults, which indicates the potential roles of fetuin-B in fish organogenesis.
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Wuhan, 430072, P.R. China.
authors.forename: Jing-Xia
authors.initials: JX
authors.lastname: Liu
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Zhai
authors.forename: Fan-Suo
authors.initials: FS
authors.lastname: Geng
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Xia
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: Gui