title: Preparation and application of monoclonal antibody against hNDRG2.
abstract: The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5alpha as a 6x His-tagged protein. The purified 6x His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.
authors.affiliation: Department of Biochemistry and Molecular Biology, The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Xinping
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Hu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
authors.forename: Wenghong
authors.initials: W
authors.lastname: Zhang
authors.forename: Xuesong
authors.initials: X
authors.lastname: Liu
authors.forename: Fuyang
authors.initials: F
authors.lastname: Li
authors.forename: Yuanqiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Libo
authors.initials: L
authors.lastname: Yao