title: Temporal regulation of ephrin/Eph signalling is required for the spatial patterning of the mammalian striatum.
abstract: Brain structures, whether mature or developing, display a wide diversity of pattern and shape, such as layers, nuclei or segments. The striatum in the mammalian forebrain displays a unique mosaic organization (subdivided into two morphologically and functionally defined neuronal compartments: the matrix and the striosomes) that underlies important functional features of the basal ganglia. Matrix and striosome neurons are generated sequentially during embryonic development, and segregate from each other to form a mosaic of distinct compartments. However, the molecular mechanisms that underlie this time-dependent process of neuronal segregation remain largely unknown. Using a novel organotypic assay, we identified ephrin/Eph family members as guidance cues that regulate matrix/striosome compartmentalization. We found that EphA4 and its ephrin ligands displayed specific temporal patterns of expression and function that play a significant role in the spatial segregation of matrix and striosome neurons. Analysis of the striatal patterning in ephrin A5/EphA4 mutant mice further revealed the requirement of EphA4 signalling for the proper sorting of matrix and striosome neuronal populations in vivo. These data constitute the first identification of genes involved in striatal compartmentalization, and reveal a novel mechanism by which the temporal control of guidance cues enables neuronal segregation, and thereby the generation of complex cellular patterns in the brain.
authors.affiliation: Université Libre de Bruxelles (U.L.B.), IRIBHM (Interdisciplinary Research), Brussels, Belgium.
authors.forename: Lara
authors.initials: L
authors.lastname: Passante
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gaspard
authors.forename: Mélanie
authors.initials: M
authors.lastname: Degraeve
authors.forename: Jonas
authors.initials: J
authors.lastname: Frisén
authors.forename: Klas
authors.initials: K
authors.lastname: Kullander
authors.forename: Viviane
authors.initials: V
authors.lastname: De Maertelaer
authors.forename: Pierre
authors.initials: P
authors.lastname: Vanderhaeghen