title: DNA distortion and specificity in a sequence-specific endonuclease.
abstract: Five new structures of the Q138F HincII enzyme bound to a total of three different DNA sequences and three different metal ions (Ca(2+), Mg(2+), and Mn(2+)) are presented. While previous structures were produced from soaking Ca(2+) into preformed Q138F HincII/DNA crystals, the new structures are derived from cocrystallization with Ca(2+), Mg(2+), or Mn(2+). The Mn(2)(+)-bound structure provides the first view of a product complex of Q138F HincII with cleaved DNA. Binding studies and a crystal structure show how Ca(2+) allows trapping of a Q138F HincII complex with noncognate DNA in a catalytically incompetent conformation. Many Q138F HincII/DNA structures show asymmetry, despite the binding of a symmetric substrate by a symmetric enzyme. The various complexes are fit into a model describing the different conformations of the DNA-bound enzyme and show how DNA conformational energetics determine DNA-cleavage rates by the Q138F HincII enzyme.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721, USA.
authors.forename: Andrea C
authors.initials: AC
authors.lastname: Babic
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Little
authors.forename: Veena M
authors.initials: VM
authors.lastname: Manohar
authors.forename: Jurate
authors.initials: J
authors.lastname: Bitinaite
authors.forename: Nancy C
authors.initials: NC
authors.lastname: Horton