title: Subtype analysis of stx1, stx2 and eae genes in Shiga toxin-producing Escherichia coli (STEC) and typical and atypical enteropathogenic E. coli (EPEC) from lambs in India.
abstract: Seventy-five Escherichia coli isolates with at least one targeted virulence gene were recovered from 338 lambs with (n=230) and without (n=108) diarrhoea. The isolates belonged to 36 different serogroups. Shiga toxin-producing E. coli (STEC) was isolated from 9.6% of lambs with and 24.1% of lambs without diarrhoea. Enteropathogenic E. coli (EPEC) was isolated from 6.1% of lambs with and 11.1% of lambs without diarrhoea. Of 26 EPEC isolates, seven were typical (positive for bfpA), and, of 34 stx(1) positive isolates, 25 were subtyped as stx(1c). Five of 29 stx(2) positive isolates were subtyped as stx(2d) and two as stx(2c). Seven of 45 eae positive isolates were subtyped as eae subtype zeta (eaezeta). This appears to be the first report of the isolation of typical EPEC from sheep in India.
authors.affiliation: Division of Veterinary Microbiology and Immunology, SK University of Agricultural Sciences and Technology of Kashmir, Shuhama, Alusteng, Srinagar 190006, India. shakilwani@gmail.com
authors.forename: S A
authors.initials: SA
authors.lastname: Wani
authors.forename: I
authors.initials: I
authors.lastname: Hussain
authors.forename: I
authors.initials: I
authors.lastname: Fayaz
authors.forename: M A
authors.initials: MA
authors.lastname: Mir
authors.forename: Y
authors.initials: Y
authors.lastname: Nishikawa