title: Quantitative proteomic analysis of bean plants infected by a virulent and avirulent obligate rust fungus.
abstract: Plants appear to have two types of active defenses, a broad-spectrum basal system and a system controlled by R-genes providing stronger resistance to some pathogens that break the basal defense. However, it is unknown if the systems are separate entities. Therefore, we analyzed proteins from leaves of the dry bean crop plant Phaseolus vulgaris using a high-throughput liquid chromatography tandem mass spectrometry method. By statistically comparing the amounts of proteins detected in a single plant variety that is susceptible or resistant to infection, depending on the strains of a rust fungus introduced, we defined basal and R-gene-mediated plant defenses at the proteomic level. The data reveal that some basal defense proteins are potential regulators of a strong defense weakened by the fungus and that the R-gene modulates proteins similar to those in the basal system. The results satisfy a new model whereby R-genes are part of the basal system and repair disabled defenses to reinstate strong resistance.
authors.affiliation: Soybean Genomics and Improvement Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705, USA.
authors.forename: Joohyun
authors.initials: J
authors.lastname: Lee
authors.forename: Jian
authors.initials: J
authors.lastname: Feng
authors.forename: Kimberly B
authors.initials: KB
authors.lastname: Campbell
authors.forename: Brian E
authors.initials: BE
authors.lastname: Scheffler
authors.forename: Wesley M
authors.initials: WM
authors.lastname: Garrett
authors.forename: Sandra
authors.initials: S
authors.lastname: Thibivilliers
authors.forename: Gary
authors.initials: G
authors.lastname: Stacey
authors.forename: Daniel Q
authors.initials: DQ
authors.lastname: Naiman
authors.forename: Mark L
authors.initials: ML
authors.lastname: Tucker
authors.forename: M A
authors.initials: MA
authors.lastname: Pastor-Corrales
authors.forename: Bret
authors.initials: B
authors.lastname: Cooper