title: Bacterial diversity and structural changes of oyster shell during 1-year storage.
abstract: We examined the biodiversity of bacteria associated with oyster-shell waste during a 1-year storage period using 16S ribosomal DNA analysis. Temperature variation and structural changes of oyster shell were observed during storage. Initial and final temperatures were at 16-17 degrees C, but a high temperature of about 60 degrees C was recorded after approximately 6 months of storage. The crystal structure and nanograin of the oyster shell surface were sharp and large in size initially and became gradually blunter and smaller over time. Phylogenetic analysis revealed that Firmicutes were dominant in the oyster-shell waste initially, during the high-temperature stage, and after 1 year of storage (making up >65% of the biodiversity at all three sampling times). Bacillus licheniformis was presumed as the predominate Firmicutes present. These bacteria are likely to have important roles in the biodegradation of oyster shell.
authors.affiliation: Division of Applied Life Science (BK21 Program), Gyeongsang National University, Chinju, 660-701, Republic of Korea.
authors.forename: Shah Md Asraful
authors.initials: SM
authors.lastname: Islam
authors.forename: Sun Joo
authors.initials: SJ
authors.lastname: Hong
authors.forename: Kye Man
authors.initials: KM
authors.lastname: Cho
authors.forename: Renukaradhya K
authors.initials: RK
authors.lastname: Math
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Heo
authors.forename: Young Han
authors.initials: YH
authors.lastname: Lee
authors.forename: Ki Sang
authors.initials: KS
authors.lastname: Lee
authors.forename: Han Dae
authors.initials: HD
authors.lastname: Yun