MIFlowCyt: the minimum information about a Flow Cytometry Experiment.
A fundamental tenet of scientific research is that published results are open to independent validation and refutation. Minimum data standards aid data providers, users, and publishers by providing a specification of what is required to unambiguously interpret experimental findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a cross-disciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the FCM experiment, the configuration of the instrument used to perform the assays, and the data processing approaches used to interpret the primary output data. MIFlowCyt has been adopted as a standard by ISAC, representing the FCM scientific community including scientists as well as software and hardware manufacturers. Adoptionof MIFlowCyt by the scientific and publishing communities will facilitate third-party understanding and reuse of FCM data.
Lee
Jamie A
JA
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Spidlen
Josef
J
Boyce
Keith
K
Cai
Jennifer
J
Crosbie
Nicholas
N
Dalphin
Mark
M
Furlong
Jeff
J
Gasparetto
Maura
M
Goldberg
Michael
M
Goralczyk
Elizabeth M
EM
Hyun
Bill
B
Jansen
Kirstin
K
Kollmann
Tobias
T
Kong
Megan
M
Leif
Robert
R
McWeeney
Shannon
S
Moloshok
Thomas D
TD
Moore
Wayne
W
Nolan
Garry
G
Nolan
John
J
Nikolich-Zugich
Janko
J
Parrish
David
D
Purcell
Barclay
B
Qian
Yu
Y
Selvaraj
Biruntha
B
Smith
Clayton
C
Tchuvatkina
Olga
O
Wertheimer
Anne
A
Wilkinson
Peter
P
Wilson
Christopher
C
Wood
James
J
Zigon
Robert
R
Scheuermann
Richard H
RH
Brinkman
Ryan R
RR