title: Binding properties of water-soluble carbosilane dendrimers.
abstract: Dendrimers have been proposed as new carriers for drug delivery. They have distinctive characteristics, such as uniform and controlled size, monodispersity and modifiable surface group functionality, which make them extremely useful for biomedical applications. In this study, the binding capacity of water-soluble carbosilane dendrimers was examined. A double fluorimetric titration method with 1-anilinonaphthalene-8-sulphonic acid (ANS) was used to estimate the binding constant and the number of binding centers per dendrimer molecule. The data obtained suggest that ANS interacts non-covalently with the dendrimers. Second generation dendrimers have an open, asymmetric structure that allows them to encapsulate ANS. The ability of the polymers to interact with DNA was assessed by an ethidium bromide (EB) displacement assay. All the dendrimers studied bound to DNA in competition with EB, though the strength of binding varied. Dendrimer interactions with a protein (BSA) were tested using fluorescence quenchers. The dendrimers caused no conformation change in the protein, indicating that interactions between carbosilane dendrimers and BSA are weak and occur preferentially at the protein surface.
authors.affiliation: Department of General Biophysics, University of Lodz, 12/16 Banacha St., 90-237, Lodz, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Pedziwiatr
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.forename: Louis
authors.initials: L
authors.lastname: Chonco
authors.forename: Paula
authors.initials: P
authors.lastname: Ortega
authors.forename: F Javier
authors.initials: FJ
authors.lastname: de la Mata
authors.forename: Rafael
authors.initials: R
authors.lastname: Gómez
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.forename: Ma Angeles
authors.initials: MA
authors.lastname: Muñoz-Fernandez