title: Gene expression profiling reveals alteration of caspase 6 and 14 transcripts in normal skin of keloid-prone patients.
abstract: Excessive scar formation in keloids points to altered tissue modeling and repair mechanisms. Dysregulation of cytokine and apoptotic cascades and their downstream signaling pathways might have a role in keloid development. Total RNA was isolated from biopsied keloidal tissue and adjacent normal skin of black patients, white patient's scars, and normal skin of black and white patients, with normal wound healing. Apoptosis, cytokine and NFkB pathway microarrays were used to study and compare gene expression levels. Real-time PCR was used to verify microarray results in original samples and a separate, validation-set of samples. Significant differences were observed in the expression levels of members of caspase, cytokines and MAP kinase pathways, between the normal skin of keloid-prone and normal skin of keloid-resistant patients. Specifically, expression of caspase 6, and caspase 14 genes were different between normal skin of keloid-prone individuals and normal skin of keloid-resistant patients. Our results suggest that normal skin of keloid-prone individuals constitutively expresses a distinct gene profile which might contribute to their susceptibility to develop keloids.
authors.affiliation: Department of Pathology, Laboratory Medicine, Indiana University School of Medicine, Clarian Pathology Laboratory 350 W. 11th Street CPL 5044, Indianapolis, IN 46202, USA. nassiri@hemepath.com
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nassiri
authors.forename: Heather
authors.initials: H
authors.lastname: Woolery-Lloyd
authors.forename: Sharon
authors.initials: S
authors.lastname: Ramos
authors.forename: Sharon E
authors.initials: SE
authors.lastname: Jacob
authors.forename: Dijana
authors.initials: D
authors.lastname: Gugic
authors.forename: Anita
authors.initials: A
authors.lastname: Viciana
authors.forename: Paolo
authors.initials: P
authors.lastname: Romanelli
authors.forename: George
authors.initials: G
authors.lastname: Elgart
authors.forename: Brian
authors.initials: B
authors.lastname: Berman
authors.forename: Vladimir
authors.initials: V
authors.lastname: Vincek