title: KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate.
abstract: A novel gene, bla(KHM-1), encoding a metallo-beta-lactamase, KHM-1, was cloned from a clinical isolate of Citrobacter freundii resistant to most beta-lactam antibiotics. Escherichia coli expressing bla(KHM-1) was resistant to all broad-spectrum beta-lactams except for monobactams and showed reduced susceptibility to carbapenems. Recombinant KHM-1 exhibited EDTA-inhibitable hydrolytic activity against most beta-lactams, with an overall preference for cephalosporins.
authors.affiliation: Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Shinjuku, Tokyo, Japan.
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Sekiguchi
authors.forename: Koji
authors.initials: K
authors.lastname: Morita
authors.forename: Tomoe
authors.initials: T
authors.lastname: Kitao
authors.forename: Noboru
authors.initials: N
authors.lastname: Watanabe
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Okazaki
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.forename: Masato
authors.initials: M
authors.lastname: Kanamori
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae