title: HIV-1 ribonuclease H inhibitory phenolic glycosides from Eugenia hyemalis.
abstract: Three new galloyl arbutins, hyemalosides A-C (1-3), along with nine known compounds were isolated from the evergreen tree Eugenia hyemalis. The structures of compounds 1-3 were determined by analysis of NMR and MS data. Compounds 1-3 inhibited HIV-1 RNase H in vitro with IC50 values of 1.46, >18, and 1.19 microM, respectively. However, in a XTT-based cell viability assay using the human T-cell line CEM-SS infected with HIV-1 RT, none of the compounds inhibited the cytopathic effect of HIV-1 infection at the highest dose tested (20 microg/mL).
authors.affiliation: Molecular Targets Development Program, SAIC-Frederick, Inc., Frederick, Maryland 21702, USA.
authors.forename: Heidi R
authors.initials: HR
authors.lastname: Bokesch
authors.forename: Antony
authors.initials: A
authors.lastname: Wamiru
authors.forename: Stuart F J
authors.initials: SF
authors.lastname: Le Grice
authors.forename: John A
authors.initials: JA
authors.lastname: Beutler
authors.forename: Tawnya C
authors.initials: TC
authors.lastname: McKee
authors.forename: James B
authors.initials: JB
authors.lastname: McMahon