Lactobacillus plantarum response to inorganic carbon concentrations: PyrR2-dependent and -independent transcription regulation of genes involved in arginine and nucleotide metabolism.
Lactobacillus plantarum susbp. plantarum is a capnophilic Gram-positive heterotroph with optimal growth in 4 % CO(2)-enriched air. At low inorganic carbon (C(i)) concentrations, the pyr genes encoding the enzymes of the pyrimidine biosynthetic pathway were overexpressed, in agreement with a previous study showing that these genes are regulated at the transcription level in response to C(i) via a PyrR(2)-mediated mechanism. A previous study of high-CO(2)-requiring (HCR) mutants revealed an unknown genetic link between arginine regulation and C(i)-dependent nutritional needs. To better understand L. plantarum's adaptation to C(i) availability, additional C(i)-responsive genes were sought in the arginine biosynthetic pathway (arg and car genes) using slot-blot hybridization and a proteomic differential 2D gel electrophoresis (DIGE) global approach. Besides the nine pyr-encoded proteins, 16 new Icr (inorganic-carbon-regulated) proteins accumulated differentially in response to C(i) availability, suggesting that the C(i) response involves several metabolic pathways and adaptation processes. Among these Icr proteins only argininosuccinate lyase, encoded by argH, was involved in arginine biosynthesis. Three proteins involved in the purine biosynthetic pathway and nucleotide conversion, adenylate kinase (Adk), GMP synthase (GuaA), and IMP dehydrogenase (GuaB), accumulated differentially in response to changes in C(i) levels. Expression of the Icr protein-encoding genes argH and guaB was regulated at the transcription level or by RNA stability in response to C(i) availability, as previously demonstrated for the pyr genes. However, PyrR(2) was not essential for the C(i)-regulated transcription of argH and guaB, demonstrating that PyrR(2) modulates only a subset of C(i)-regulated genes. These results suggest that the C(i) response may involve at least two regulatory mechanisms in L. plantarum.
Bringel
Françoise
F
Université Louis-Pasteur Strasbourg-I, Strasbourg, France.|CNRS, UMR7156, Génétique Moléculaire, Génomique Microbiologie, Département Microorganismes, Génomes, Environnement, 28 Rue Goethe, 67083 Strasbourg, France.
Hammann
Philippe
P
CNRS, FRC 1589, Plateforme Protéomique Esplanade, 15 Rue René Descartes, 67084 Strasbourg, France.
Kugler
Valérie
V
Université Louis-Pasteur Strasbourg-I, Strasbourg, France.|CNRS, UMR7156, Génétique Moléculaire, Génomique Microbiologie, Département Microorganismes, Génomes, Environnement, 28 Rue Goethe, 67083 Strasbourg, France.
Arsène-Ploetze
Florence
F
Université Louis-Pasteur Strasbourg-I, Strasbourg, France.|CNRS, UMR7156, Génétique Moléculaire, Génomique Microbiologie, Département Microorganismes, Génomes, Environnement, 28 Rue Goethe, 67083 Strasbourg, France.