title: Heterologous expression ofa histone-like protein from Streptococcus intermedius in Escherichia coli alters the nucleoid structure and inhibits the growth of E. coli.
abstract: Escherichia coli failed to survive after transformation with a Streptococcus intermedius histone-like protein gene (Si-hlp) and its promoter-harbored plasmid. The promoter function of Si-hlp in E. coli was determined using enhanced green fluorescence protein (egfp) gene as a reporter. The inhibitory effect of Si-HLP on E. coli viability was verified by a tetracycline-inducible gene expression system. Further study suggested that Si-HLP may alter the bacterial nucleoid structure, leading to the growth inhibition of E. coli.
authors.forename: Dali
authors.initials: D
authors.lastname: Liu
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yumoto
authors.forename: Keiji
authors.initials: K
authors.lastname: Murakami
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Hirota
authors.forename: Shizuo
authors.initials: S
authors.lastname: Kayama
authors.forename: Tomonori
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Akitake
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Tsuneko
authors.initials: T
authors.lastname: Ono
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuo
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Miyake