Application of monoclonal antibodies in the Dutch avian leukosis control programme.
Comparative testing with series of albumen samples obtained from seven Dutch poultry flocks was performed for avian leukosis virus group-specific antigen (ALV-gsa) by complement fixation test (CFT) and by a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) employing selected monoclonal antibodies directed to different ALV-p27 epitopes. The higher sensitivity for ALV-gsa detection of the DAS-ELISA resulted in increased scores of ALV-shedding hens in all seven flocks. Relatively low absorbance values seem to be associated with low rates of congenital ALV transmission.
De Boer
G F
GF
Virology Department, Central Veterinary Institute, Lelystad, The Netherlands.
Osterhaus
A D
AD
Kouwenhoven
B
B