title: DNA double-strand breaks induced by very low X-ray doses are largely due to bystander effects.
abstract: Phosphorylated ATM immunofluorescence staining was used to investigate the dose-response relationship for the number of DNA double-strand breaks (DSBs) induced in primary normal human fibroblasts irradiated with doses from 1.2 to 200 mGy. The induction of DSBs showed a supralinear dose-response relationship. Radiation-induced bystander effects may explain these findings. To test this hypothesis, the number of DSBs in cells treated with lindane, an inhibitor of radiation-induced bystander effects, prior to X irradiation was assessed; a supralinear dose-response relationship was not observed. Moreover, the number of DSBs obtained by subtracting the number of phosphorylated ATM foci in lindane-treated cells from the number of phosphorylated ATM foci in untreated cells was proportional to the dose at low doses (1.2-5 mGy) and was saturated at doses from 10-200 mGy. Thus the increase in the number of DSBs in the range of 1.2-5 mGy was largely due to radiation-induced bystander effects, while at doses >10 mGy, the DSBs may be induced mainly by dose-dependent direct radiation effects and partly by dose-independent radiation-induced bystander effects. The findings in our present study provide direct evidence of the dose-response relationship for radiation-induced bystander effects from broad-beam X rays.
authors.affiliation: Department of Environmental Health Science, Oita University of Nursing and Health Sciences, 2944-9 Megusuno, Oita 840-1201, Japan. ojima@oita-nhs.ac.jp
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Ojima
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Ban
authors.forename: Michiaki
authors.initials: M
authors.lastname: Kai