title: 2D difference gel electrophoresis of prepubertal and pubertal rat mammary gland proteomes.
abstract: Rat mammary gland proteomes at day 21 (prepubertal) and day 50 (late puberty) were compared by 2D difference gel electrophoresis. Two-hundred fifty-one spots were significantly different ( p < 0.05) in abundance. Peptide mass fingerprint analysis of a subset of these proteins identified two significantly over-represented classes including structural and blood proteins (increased), and metabolism-relevant proteins (reduced) in day 50 relative to day 21 glands. This is a first report of mammary gland proteome differences at these important breast cancer-relevant time-points.
authors.affiliation: Departments of Pharmacology & Toxicology, the UAB Center for Nutrient-Gene Interaction, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA. Helenkim@uab.edu
authors.forename: Helen
authors.initials: H
authors.lastname: Kim
authors.forename: Mark B
authors.initials: MB
authors.lastname: Cope
authors.forename: Richie
authors.initials: R
authors.lastname: Herring
authors.forename: Gloria
authors.initials: G
authors.lastname: Robinson
authors.forename: Landon
authors.initials: L
authors.lastname: Wilson
authors.forename: Grier P
authors.initials: GP
authors.lastname: Page
authors.forename: Stephen
authors.initials: S
authors.lastname: Barnes