Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of hypothetical protein SCO4226 from Streptomyces coelicolor A3(2).
A non-Pfam hypothetical protein SCO4226 of molecular weight 9 kDa from Streptomyces coelicolor A3(2) was overexpressed in Escherichia coli and the purified recombinant protein was crystallized using the sitting-drop vapour-diffusion method. An X-ray diffraction data set was collected to 2.0 A resolution. The crystal belonged to space group P2(1), with unit-cell parameters a = 29.67, b = 67.00, c = 34.43 A, alpha = gamma = 90.00, beta = 94.26 degrees .
Wang
Shu
S
School of Life Sciences and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, People's Republic of China.
He
Yong Xing
YX
Bao
Rui
R
Teng
Yan Bin
YB
Ye
Bo Ping
BP
Zhou
Cong Zhao
CZ