title: [Relationship between alterations of circulating cell-derived microparticles and glucocorticosteroid application].
abstract: OBJECTIVE
  To explore the relationship between the alterations of circulating cell-derived microparticles (MPs) and large-dose glucocorticosteroid application.
  
  
  METHODS
  Peripheral blood samples were collected from 33 patients with history of large-dose glucocorticosteroid application (glucocorticosteroid group) and 24 age-, sex-, and race-matched healthy controls (healthy control group). Platelet-poor plasma was obtained by centrifugation. Plasma microparticles were labeled with monoclonal antibodies of PE-conjugated mouse anti-human CD31, CD54, and FITC-conjugated mouse anti-human CD42b, CD45, CD51/61, CD105, and PE-Cy5-conjugated mouse anti-human CD62E. Cell-derived microparticles were measured by three-color flow cytometry.
  
  
  RESULTS
  The mean ranks of CD31+ MPs, CD45+ MPs, CD51/61 MPs, CD31+ CD42b+ MPs, and CD31+ CD45+ MPs of the glucocorticosteroid group were: 24.1, 25.5, 25.6, 21.6, and 24.8 respectively, all significantly lower than those of the control group (35.7, 33.8, 33.7, 39.3, and 34.8 respectively, P = 0.009, 0.019, 0.045, 0.000, and 0.009).
  
  
  CONCLUSIONS
  High-dose glucocorticosteroid application remarkably reduces plasma MPs that may be responsible for microcirculation disturbance.
authors.affiliation: Department of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, China.
authors.forename: Zeng-xin
authors.initials: ZX
authors.lastname: Gao
authors.forename: Xi-sheng
authors.initials: XS
authors.lastname: Weng
authors.forename: Gui-xing
authors.initials: GX
authors.lastname: Qiu
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.forename: Bai
authors.initials: B
authors.lastname: Shao
authors.forename: Su-ping
authors.initials: SP
authors.lastname: Mei
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.forename: Xin-yu
authors.initials: XY
authors.lastname: Yang