Glycomic profiling of invasive and non-invasive breast cancer cells.
Quantitative profiling of glycans with different structures appears essential for a better understanding of the cellular adhesion phenomena associated with malignant transformation and the underlying aberrant glycosylation of cancer cells. Using the recently developed glycomic techniques and mass-spectrometric measurements, we compare the N-linked and O-linked oligosaccharide profiles for different breast cancer cell lines with those of normal epithelial cells. Statistically significant differences in certain neutral, sialylated and fucosylated structures are readily discerned through quantitative measurements, indicating a potential of distinguishing invasive and non-invasive cancer attributes. The glycomic profile data cluster accordingly using Principal Component Analysis, verifying further glycobiological differences due to the differences between normal and cancer cell lines.
Goetz
John A
JA
Department of Chemistry, Indiana University, Bloomington, 47405, USA.
Mechref
Yehia
Y
Kang
Pilsoo
P
Jeng
Meei-Huey
MH
Novotny
Milos V
MV