A third genotype of the human parvovirus PARV4 in sub-Saharan Africa.
PARV4 is a recently discovered human parvovirus widely distributed in injecting drug users in the USA and Europe, particularly in those co-infected with human immunodeficiency virus (HIV). Like parvovirus B19, PARV4 persists in previously exposed individuals. In bone marrow and lymphoid tissue, PARV4 sequences were detected in two sub-Saharan African study subjects with AIDS but without a reported history of parenteral exposure and who were uninfected with hepatitis C virus. PARV4 variants infecting these subjects were phylogenetically distinct from genotypes 1 and 2 (formerly PARV5) that were reported previously. Analysis of near-complete genome sequences demonstrated that they should be classified as a third (equidistant) PARV4 genotype. The availability of a further near-complete genome sequence of this novel genotype facilitated identification of conserved novel open reading frames embedded in the ORF2 coding sequence; one encoded a putative protein with identifiable homology to SAT proteins of members of the genus Parvovirus.
Simmonds
Peter
P
Centre for Infectious Diseases, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK.
Douglas
Jill
J
Centre for Infectious Diseases, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK.
Bestetti
Giovanna
G
Infectious Diseases and Tropical Medicine and Pathology Departments, Luigi Sacco Hospital, Milano, Italy.
Longhi
Erika
E
Infectious Diseases and Tropical Medicine and Pathology Departments, Luigi Sacco Hospital, Milano, Italy.
Antinori
Spinello
S
Infectious Diseases and Tropical Medicine and Pathology Departments, Luigi Sacco Hospital, Milano, Italy.
Parravicini
Carlo
C
Infectious Diseases and Tropical Medicine and Pathology Departments, Luigi Sacco Hospital, Milano, Italy.
Corbellino
Mario
M
Infectious Diseases and Tropical Medicine and Pathology Departments, Luigi Sacco Hospital, Milano, Italy.