title: Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF).
abstract: Human metallothionein-3 (hMT3), also named as human neuronal growth inhibitory factor (hGIF), can inhibit the outgrowth of embryonic cortical neurons in the presence of brain extracts. In order to systematically study the structure-property-reactivity-function relationship of hGIF, our laboratory designed a series of mutants and studied their structure, property, reactivity and functions by a series of chemical and biological tools including UV spectroscopy, CD spectroscopy, NMR, chemical reaction and primary neuronal culture assays. In summary, we concluded that the bioactivity of hGIF was regulated by multiple factors, including the (6)CPCP(9) motif, an additional threonine insert at sequence position 5, domain-domain interactions, the structure and stability of the metal-thiolate cluster and the linker. Our studies provide more and more evidences which revealed that the bioactivity of hGIF is mainly related to the essential metal release and its characteristic conformation.
authors.affiliation: Chemical Biology Laboratory, Department of Chemistry, Fudan University, Shanghai 200433, China.
authors.forename: Zhi-Chun
authors.initials: ZC
authors.lastname: Ding
authors.forename: Qi
authors.initials: Q
authors.lastname: Zheng
authors.forename: Bin
authors.initials: B
authors.lastname: Cai
authors.forename: Feng-Yun
authors.initials: FY
authors.lastname: Ni
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Yu
authors.forename: Xing-Chen
authors.initials: XC
authors.lastname: Teng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Hou-Ming
authors.initials: HM
authors.lastname: Wu
authors.forename: Hong-Zhe
authors.initials: HZ
authors.lastname: Sun
authors.forename: Ming-Jie
authors.initials: MJ
authors.lastname: Zhang
authors.forename: Xiang-Shi
authors.initials: XS
authors.lastname: Tan
authors.forename: Zhong-Xian
authors.initials: ZX
authors.lastname: Huang