Mixture design as first step for improved glutaminase production in solid-state fermentation by isolated Bacillus.
OBJECTIVE
Investigation of mixture-design impact on glutaminase production by isolated Bacillus sp.


RESULTS
An augmented simplex centroid design was used to optimize a three (wheat bran, Bengal gram husk and palm seed fibre) component mixture for glutaminase production. Selected substrate materials showed impact on glutaminase production values at individual level by Bengal gram husk [2789 U gds(-1) (gram dry substrate] and in two-level combination with wheat bran and Bengal gram husk (maximum of 3300 U gds(-1)).


CONCLUSIONS
Bengal gram husk is the most suitable substrate medium for glutaminase production by Bacillus sp. Maximum glutaminase production is achieved using solid-substrate mixture at two-level combinations in the ratio of 66 : 34 for Bengal gram husk and wheat bran, respectively.


CONCLUSIONS
The present study has significance in large-scale production of glutaminase at commercial level with the use of multi-substrate rather than single-substrate/support material.
Sathish
T
T
Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India.
Lakshmi
G S
GS
Rao
Ch S
ChS
Brahmaiah
P
P
Prakasham
R S
RS