title: Cellobiose is extensively digested in the small intestine by beta-galactosidase in rats.
abstract: OBJECTIVE
  The bioavailability of cellobiose (CEB) was investigated with respect to small intestinal digestibility and cecal fermentation in rats. Further, whether small intestinal beta-galactosidase is responsible for the hydrolysis of CEB was examined.
  
  
  METHODS
  Ileorectostomized rats were fed diets including 6% CEB or fructo-oligosaccharide with or without 0.1% neomycin in drinking water for 7 d. The fecal excretion of the respective oligosaccharides was determined. In vitro digestion of CEB and lactose was characterized using mucosal enzymes of the small intestine from suckling and adult rats. Cecal fermentation in normal rats fed a control diet or a diet including 3% or 6% CEB for 14 d was examined.
  
  
  RESULTS
  The small intestinal digestibility of CEB was 64%, irrespective of the presence of neomycin in drinking water, whereas the digestibility of fructo-oligosaccharide differed significantly between groups administered (26%) or not administered (35%) neomycin. The in vitro digestibility of lactose (62%) and CEB (36%) was three times greater with the enzymes from the suckling rats than with those from the adult rats. Michaelis constant (K(m)) and maximum velocity (V(max)) values for CEB were 25 and 7 times lower, respectively, than those for lactose. Normal rats fed the 6% CEB diet showed a greater cecal organic acid than those fed the control diet, but no differences were observed between those fed the control and 3% CEB diets.
  
  
  CONCLUSIONS
  Our results indicate that dietary CEB was extensively digested in the small intestine by beta-galactosidase in rats, leading to complete digestion of CEB when dietary supplementation was limited.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan. actmori@agr.shizuoka.ac.jp
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Morita
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ozawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ito
authors.forename: Sugiyama
authors.initials: S
authors.lastname: Kimio
authors.forename: Shuhachi
authors.initials: S
authors.lastname: Kiriyama