title: Strain-specific seroepidemiology and reinfection of cytomegalovirus.
abstract: Although there have been some reports describing the serostatus of cytomegalovirus, strain-specific antibody responses and their distribution remain unknown. In this study, ELISA using fusion proteins encompassing epitope of glycoprotein H from both AD169 and Towne strains was used to test 352 blood donors. Of these 352 donors, 207 were analyzed for strain-specific glycoprotein H antibodies. Of the 44 donors whose serum contained antibodies against both AD169 and Towne, 27 (60%) were aged 50 years or over (p = 0.0003). This may indicate serological evidence of reinfection with cytomegalovirus in the elder population. The nucleotide sequence analysis of cytomegalovirus glycoprotein H from the peripheral blood of the cytomegalovirus-positive renal transplant recipients showed that our strain-specific ELISA can reveal cytomegalovirus reinfection after transplantation.
authors.affiliation: Department of Microbiology, Fukushima Medical University, Fukushima, Japan. keikun@fmu.ac.jp
authors.forename: Kei
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Tadahiko
authors.initials: T
authors.lastname: Tokumoto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Shirakawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kushida
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Yanagida
authors.forename: Keiichi
authors.initials: K
authors.lastname: Shishido
authors.forename: Ken
authors.initials: K
authors.lastname: Aikawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamaguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Toma
authors.forename: Kazunari
authors.initials: K
authors.lastname: Tanabe
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzutani