Larvicidal activity of lectins from Myracrodruon urundeuva on Aedes aegypti.
Aedes aegypti transmits etiologic agents of yellow fever and dengue. Vaccine for dengue virus is not available and vector control is essential to minimize dengue incidence. This report deals with the larvicidal activity of lectins isolated from Myracrodruon urundeuva bark (MuBL) and heartwood (MuHL). The lectins were isolated by ammonium sulphate treatment of crude extracts followed by chromatography on chitin. MuBL and MuHL were evaluated by electrophoresis under native (PAGE) and denaturing conditions (SDS-PAGE). Carbohydrate specificity of lectins was evaluated by hemagglutinating activity (HA) inhibition assay using N-acetyl-d-glucosamine and by affinity chromatography on N-acetyl-D-glucosamine immobilized in agarose gel. Larvicidal activity against A. aegypti was investigated with the extracts, salt fractions and isolated lectins. MuBL and MuHL were characterized by PAGE as basic proteins of molecular masses of 14.0 and 14.4 kDa, respectively. The interaction of lectins with N-acetylglucosamine was detected by inhibition of HA by monosaccharide and lectin adsorptions on N-acetyl-D-glucosamine matrix. All M. urundeuva preparations promoted larvae mortality. LC16, LC50 and LC84 values of 0.077, 0.125, 0.173 for MuBL and 0.03, 0.04 and 0.05 mg/mL for MuHL were obtained. To our knowledge this is the first report of larvicidal activity of lectins against A. aegypti.
Sá
Roberto Araújo
RA
Departamento de Química Fundamental, CCEN, Universidade Federal de Pernambuco, Cidade Universitária, 50670-901, Recife, Pernambuco, Brazil.
Santos
Nataly Diniz de Lima
ND
da Silva
Clécia Sipriano Borges
CS
Napoleão
Thiago Henrique
TH
Gomes
Francis Soares
FS
Cavada
Benildo Sousa
BS
Coelho
Luana Cassandra Breitenbach Barroso
LC
Navarro
Daniela Maria do Amaral Ferraz
DM
Bieber
Lothar Wilhelm
LW
Paiva
Patrícia Maria Guedes
PM