Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF).
Human metallothionein-3 (hMT3), also named as human neuronal growth inhibitory factor (hGIF), can inhibit the outgrowth of embryonic cortical neurons in the presence of brain extracts. In order to systematically study the structure-property-reactivity-function relationship of hGIF, our laboratory designed a series of mutants and studied their structure, property, reactivity and functions by a series of chemical and biological tools including UV spectroscopy, CD spectroscopy, NMR, chemical reaction and primary neuronal culture assays. In summary, we concluded that the bioactivity of hGIF was regulated by multiple factors, including the (6)CPCP(9) motif, an additional threonine insert at sequence position 5, domain-domain interactions, the structure and stability of the metal-thiolate cluster and the linker. Our studies provide more and more evidences which revealed that the bioactivity of hGIF is mainly related to the essential metal release and its characteristic conformation.
Ding
Zhi-Chun
ZC
Chemical Biology Laboratory, Department of Chemistry, Fudan University, Shanghai 200433, China.
Zheng
Qi
Q
Cai
Bin
B
Ni
Feng-Yun
FY
Yu
Wen-Hao
WH
Teng
Xing-Chen
XC
Gao
Yuan
Y
Liu
Fang
F
Chen
Dong
D
Wang
Yang
Y
Wu
Hou-Ming
HM
Sun
Hong-Zhe
HZ
Zhang
Ming-Jie
MJ
Tan
Xiang-Shi
XS
Huang
Zhong-Xian
ZX