title: Immunomodulating activities of water-soluble exopolysaccharides obtained from submerged culture of Lentinus lepideus.
abstract: Immunomodulating activities of water-soluble exopolysaccharides (LL-EX) obtained from submerged mycelial culture of Lentinus lepideus were studied and their effectiveness was compared with lipopolysaccharide (LPS). The influence of the LL-EX on macrophage cellular lysosomal enzyme activity was to stimulate up to 267%, 392%, and 464% at the level of 10, 50, and 100 microg/ml, respectively. When the LL-EX was further fractionated into LL-Fr.I and Fr.II by Sepharose CL-6B gel chromatography, the cellular lysosomal enzyme activity of LL-Fr.II (2.1- fold) was higher than Fr.I (1.2-fold). Moreover, both LL-Fr.I and Fr.II stimulated the cytokines IL-1beta, TNF-alpha, and IL-6 in macrophages. In mixed lymphocyte reaction, LL-Fr.I and Fr.II enhanced the splenocyte proliferation up to 1.2-fold and 1.4-fold (50 microg/ml), respectively, stimulating only T lymphocytes. The fractions of LL-EX not show any direct toxicity against human gastric adenocarcinoma cell (AGS). The molecular masses of LL-Fr.I and Fr.II were estimated to be about 1,986 kDa and 21 kDa, respectively. The total sugar and protein contents of the two fractions were 84.97% and 69.88% and 15.03% and 30.12%, respectively. The sugar and amino acid compositions of the LL-Fr.I and Fr.II were also analyzed in detail.
authors.affiliation: Department of Biotechnology, Daegu University, Gyeongsan 712-714, Korea.
authors.forename: Yu-Sun
authors.initials: YS
authors.lastname: Jung
authors.forename: Byung-Keun
authors.initials: BK
authors.lastname: Yang
authors.forename: Yong-Tae
authors.initials: YT
authors.lastname: Jeong
authors.forename: Rezuanul
authors.initials: R
authors.lastname: Islam
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Kim
authors.forename: Chi-Hyun
authors.initials: CH
authors.lastname: Song