Comparative proteomic analysis of peripheral blood mononuclear cells from atopic dermatitis patients and healthy donors.
Atopic dermatitis (AD) is a chronic inflammatory skin disease that induces changes in various inflammatory skin cells. The prevalence of AD is as high as 18% in some regions of the world, and is steadily rising. However, the pathophysiology of AD is poorly understood. To identify the proteins involved in AD pathogenesis, a comparative proteomic analysis of protein expression in peripheral blood mononuclear cells isolated from AD patients and healthy donors was conducted. Significant changes were observed in the expressions of fourteen proteins, including the vinculin, PITPNB, and Filamin A proteins. Among the proteins, alpha-SNAP and FLNA decreased significantly, and PITPNB increased significantly in AD patients compared with control subjects; these findings were further confirmed by real-time PCR and Western blot analysis. The comparative proteome data may provide a valuable clue to further understand AD pathogenesis, and several differentially regulated proteins may be used as biomarkers for diagnosis and as target proteins for the development of novel drugs.
Kim
Won Kon
WK
Translational Research Center, KRIBB, Daejeon, Korea.
Cho
Hyun Ju
HJ
Ryu
Su In
SI
Hwang
Hyang-Ran
HR
Kim
Do-Hyung
DH
Ryu
Hye Young
HY
Chung
Jin Woong
JW
Kim
Tae-Yoon
TY
Park
Byoung Chul
BC
Bae
Kwang-Hee
KH
Ko
Yong
Y
Lee
Sang Chul
SC