title: Disappearance of immunoreactive glycinin and beta-conglycinin in the digestive tract of piglets.
abstract: Soybean allergy represents a health threat to human and animals. Glycinin and beta-conglycinin, the main storage proteins in soybean, have been identified as major food/ feed allergens. The present study was conducted to investigate the disappearance of immunoreactive glycinin and beta-conglycinin in the digestive processes of piglets. Twelve crossbred piglets, weaned at 21 days of age, were allocated to three dietary treatments in a complete block design, each treatment with four replicates (female/male = 1:1). From day 22-28, the control group was fed diets without leguminous products, while the two treatment groups received diets containing 2.2% purified glycinin or beta-conglycinin. All piglets were slaughtered at 29 days of age and digesta was sampled from stomach, middle jejunum, caecum and colon. Results indicated that immunoreactive glycinin and beta-conglycinin decreased as the digesta descended down the digestive tract to 0.12% and 0.47%, respectively. Little immunoreactive glycinin was found in the digesta of caecum and colon, while immunoreactive beta-conglycinin was detected in the colon. Along the whole digestive tract the disappearance of immunoreactive glycinin was significantly higher than beta-conglycinin (p < 0.05).
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Guixin
authors.initials: G
authors.lastname: Qin
authors.forename: Zewei
authors.initials: Z
authors.lastname: Sun
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Nan
authors.initials: N
authors.lastname: Bao
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Bolin
authors.initials: B
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: Ling
authors.initials: L
authors.lastname: Sun