The cloning and expression of  alpha-tubulin in  Monochamus alternatus.
The Japanese pine sawyer Monochamus alternatus is one of the major forest pests. It damages pine directly and transfers the nematode Bursaphelenchus xylophilus to pine wood; resulting in serious economic losses around the world every year. Alpha-tubulin is one of most important proteins in most species. We cloned a ubiquitously expressed M. alternatus alpha-tubulin gene and analysed its nucleotides and protein structure; its sequence characters are consistent with what have been reported in other insects. The alignment of proteins showed that there is high homology of alpha-tubulin between M. alternatus and other species. Western blot and immunocytochemistry analyses suggested a common epitope of alpha-tubulin between M. alternatus and Strongylcentrotus purpuratus. We also expressed the protein in Escherichia coli for further functional studies.
Song
L
L
Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Beijing,China.
Liu
X-X
XX
Zhang
Y-A
YA
Zhang
Q-W
QW
Zhao
Z-W
ZW