title: Regulation of hormone-sensitive lipase expression by saturated fatty acids and hormones in bovine mammary epithelial cells.
abstract: Hormone-sensitive lipase was firstly identified as an epinephrine-induced lipase in adipocyte. HSL mRNA was detected by RT-PCR in cloned bovine mammary epithelial cells (bMEC) and bovine lactating mammary gland. Saturated fatty acids (stearate and palmitate), but not unsaturated fatty acids (oleate and linoleate) induced up-regulation of HSL mRNA in a time- and concentration-dependent manner in bMEC. Treatment with insulin (5-10 ng/ml), dexamethasone (50-250 nM) or GH (50 ng/ml) induced down-regulation of HSL. These results suggest that HSL was regulated by fatty acids and some hormones in mammary epithelial cells and thereby play an important role of lipid and energy metabolism.
authors.affiliation: Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Amamiyamachi, Sendai 981-8555, Japan. yonet@bios.tohoku.ac.jp
authors.forename: Tomo
authors.initials: T
authors.lastname: Yonezawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Haga
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Katoh
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Obara