Alternative immunofluorescent labeling of Cryptosporidium parvum in water samples using semiconductor quantum dots.
The application of immunofluorescent labeling using quantum dots for detection of inactivated Cryptosporidium parvum oocysts in spiked water samples (reservoir water, treated wastewater effluent, permeate of a membrane bioreactor, and tap water) provided more consistent results compared with the organic fluorophores label. The varying degree of particles present in the different water samples (with turbidity ranging from 0.2 to 6.1 NTU) in nonconcentrated water samples had insignificant interference on the labeled counts (2-sample t-tests, p > 0.236) using the quantum dot label, while the quantum dot label provided an advantage of approximately 50% lower interference in concentrated water samples compared with the organic fluorophores label.
Lee
Lai Yoke
LY
Division of Environmental Science and Engineering, National University of Singapore, Singapore.
Hu
Jiang Yong
JY
Ong
Say Leong
SL
Ng
How Yong
HY
Wong
Shih Wei
SW
Feng
Yaoyu
Y
Tan
Xiaolan
X