title: Modelling small-patterned neuronal networks coupled to microelectrode arrays.
abstract: Cultured neurons coupled to planar substrates which exhibit 'well-defined' two-dimensional network architectures can provide valuable insights into cell-to-cell communication, network dynamics versus topology, and basic mechanisms of synaptic plasticity and learning. In the literature several approaches were presented to drive neuronal growth, such as surface modification by silane chemistry, photolithographic techniques, microcontact printing, microfluidic channel flow patterning, microdrop patterning, etc. This work presents a computational model fit for reproducing and explaining the dynamics exhibited by small-patterned neuronal networks coupled to microelectrode arrays (MEAs). The model is based on the concept of meta-neuron, i.e., a small spatially confined number of actual neurons which perform single macroscopic functions. Each meta-neuron is characterized by a detailed morphology, and the membrane channels are modelled by simple Hodgkin-Huxley and passive kinetics. The two main findings that emerge from the simulations can be summarized as follows: (i) the increasing complexity of meta-neuron morphology reflects the variations of the network dynamics as a function of network development; (ii) the dynamics displayed by the patterned neuronal networks considered can be explained by hypothesizing the presence of several short- and a few long-term distance interactions among small assemblies of neurons (i.e., meta-neurons).
authors.affiliation: Neuroengineering and Bio-Nano Technology Group, Department of Biophysical and Electronic Engineering (DIBE), University of Genova, Via Opera Pia 11a, 16145 Genova, Italy. paolo.massobrio@unige.it
authors.forename: Paolo
authors.initials: P
authors.lastname: Massobrio
authors.forename: Sergio
authors.initials: S
authors.lastname: Martinoia