title: SNP typing of aldehyde dehydrogenase2 gene with Cycleave ICAN.
abstract: A simple and non-expensive platform is critical to realize on-site SNP typing. In this study we typed an SNP existing at the 487th residue of human aldehyde dehydrogenase2 [wild: Glu (GAA); mutant: Lys (AAA)] using our unique isothermal DNA amplification method, ICAN and cycling probes. Both genotypes were identified by the naked eye using a non-expensive UV transilluminator as well as with real-time PCR apparatus or a fluorescence detector. Since ICAN does not need thermal cycling, a cost- and space-limiting factor when fabricating apparatus, the combination of ICAN and cycling probes will be able to realize affordable on-site SNP typing in the near future.
authors.affiliation: Products Development Center, Takara Bio Inc., 2257, Noji, Kusatsu, Shiga 525-0055, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mukai
authors.forename: Osamu
authors.initials: O
authors.lastname: Takeda
authors.forename: Kanako
authors.initials: K
authors.lastname: Usui
authors.forename: Kiyozo
authors.initials: K
authors.lastname: Asada
authors.forename: Ikunoshin
authors.initials: I
authors.lastname: Kato