title: Analysis of green pit viper (Trimeresurus alborabris) venom protein by LC/MS-MS.
abstract: Green pit viper venom has major effect on the hematological system having a thrombin-like effect. Thus, this study is designed to analyze the composition of Trimeresurus albolabris venom by performing gel filtration and LC/MS-MS. The purified protein was then digested by trypsin, and the tryptic fragments were analyzed by iontrap spectrophotometry. This study found four types of proteins, namely jerdonitin, stejaggregin-A beta chain-1, stejnobin, and stejnihagin-A, as the components of T. albolabris venom. All of these toxins played a greater or lesser role in clot formation or otherwise contributed to cross-reactions in antivenom production.
authors.affiliation: Clinical Microscopy Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Suphan
authors.initials: S
authors.lastname: Soogarun
authors.forename: Polkit
authors.initials: P
authors.lastname: Sangvanich
authors.forename: Montri
authors.initials: M
authors.lastname: Chowbumroongkait
authors.forename: Surasak
authors.initials: S
authors.lastname: Jiemsup
authors.forename: Viroj
authors.initials: V
authors.lastname: Wiwanikit
authors.forename: Paweena
authors.initials: P
authors.lastname: Pradniwat
authors.forename: Attakorn
authors.initials: A
authors.lastname: Palasuwan
authors.forename: Jaturawat
authors.initials: J
authors.lastname: Pawinwongchai
authors.forename: Supanthitra
authors.initials: S
authors.lastname: Chanprasert
authors.forename: Tusrin
authors.initials: T
authors.lastname: Moungkote