Selective enhancement of resonance light-scattering of gold nanoparticles by glycogen.
Simple and sensitive methods for the determination of glycogen are desirable. This study was to establish the determination of glycogen based on the enhancement of the light-scattering intensity of gold nanoparticles (AuNPs) at nanogram per milliliter levels. Glycogen enhancement was independent of temperature and was highest at pH 7. The enhancement was linear from 10 to 800 ngmL(-1) with a 2 ngmL(-1) detection limit (3S/N). The standard deviation of the slope for 5 consecutive calibration runs was within 5%. Analysis of three artificial samples with varied glycogen concentrations had 98-102% recovery. Selected amino acids, glucose, lactose, BSA and trichloroacetic acid all showed minor effects on glycogen enhancement demonstrating the potential for glycogen determination in complex samples.
Xiang
Minghui
M
Key laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Xu
Xiao
X
Li
Dongxu
D
Liu
Feng
F
Li
Na
N
Li
Kean
K