title: 7-hydroxycoumarin-hemicyanine hybrids: a new class of far-red emitting fluorogenic dyes.
abstract: The design and synthesis of novel water-soluble far-red emitting phenol-based fluorophores derived from 7-hydroxycoumarin are described. These hemicyanine-coumarin hybrids display promising spectroscopic features such as large apparent Stokes shift (ranging from 60 to 140 nm) and fluorescence emission maxima between 620 and 720 nm in physiological conditions. Their utility was then illustrated by the preparation of an original fluorogenic probe of penicillin G acylase (PGA) whose fluorescence is unveiled through an enzyme-initiated domino reaction.
authors.affiliation: Equipe de Chimie Bio-Organique, Université de Rouen, Place Emile Blondel, 76821 Mont-Saint-Aignan, France.
authors.forename: Jean-Alexandre
authors.initials: JA
authors.lastname: Richard
authors.forename: Marc
authors.initials: M
authors.lastname: Massonneau
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Renard
authors.forename: Anthony
authors.initials: A
authors.lastname: Romieu