Identification of 14-3-3zeta by chemical affinity with salicylanilide inhibitors of interleukin-12p40 production.
Salicylanilides were found as selective inhibitors of interleukin-12p40 production in stimulated dendritic cells. The conversion of one of these bioactive salicylanilides into a comparably bioactive, chemically labeled derivative was achieved using a facile and systematic functional group derivatization strategy. This resulted in a tool reagent that was then employed in an affinity chromatography approach that resulted in the identification of the protein 14-3-3zeta as having selective affinity for the chromatography matrix that was derivatized with a salicylanilide that inhibited IL-12p40 production.
Boyce
Jim P
JP
Amgen Incorporated, 1201 Amgen Court West, Seattle, Washington 98119Amgen Incorporated, 1201 Amgen Court West, Seattle, Washington 98119, USA. jboyce2000@aol.com
Brown
Michael E
ME
Chin
Wilson
W
Fitzner
Jeffrey N
JN
Paxton
Raymond J
RJ
Shen
Min
M
Stevens
Tracey
T
Wolfson
Martin F
MF
Wright
Clifford D
CD