title: Mutation in a gene encoding anti-sigma factor in A. brasilense confers tolerance to elevated temperature, antibacterial peptide and PEG-200 via carotenoid synthesis.
abstract: Azospirillum brasilense Sp7 has been shown to overproduce carotenoids if the anti-sigma factor (anti-sigma(E))-encoding gene is inactivated. The anti-sigma mutant (Car-1) of A. brasilense Sp7 was more tolerant to the stresses generated by elevated temperature (40 degrees C), PEG-200 (30 mg mL(-1)) and the antibacterial agent Polymyxin-B (PMB, 25 microg mL(-1)) but not to elevated salinity (15 mg mL(-1)). Inhibition of carotenoid synthesis by diphenylamine inhibited the ability of the mutant to tolerate all the three stresses. Out of the four stress agents, only elevated temperature and salinity induced the rpoE promoter and increased the carotenoid content in Sp7 as well as in the Car-1 mutant. Comparison of the membrane permeability of the parent and the mutant by a PMB-N-phenyl-1-naphthylamine coupled assay showed that the presence of carotenoids in the mutant reduced the permeability of their membranes. Our study indicates that the carotenoid synthesis, which is under the control of extracytoplasmic function sigma factor (sigma(E)) in A. brasilense Sp7, plays a positive role in tolerating elevated temperature, the antibacterial peptide and PEG-200.
authors.affiliation: School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India.
authors.forename: Mukti Nath
authors.initials: MN
authors.lastname: Mishra
authors.forename: Nagarajan
authors.initials: N
authors.lastname: Thirunavukkarasu
authors.forename: Indra Mani
authors.initials: IM
authors.lastname: Sharma
authors.forename: Medicharla V
authors.initials: MV
authors.lastname: Jagnnadham
authors.forename: Anil K
authors.initials: AK
authors.lastname: Tripathi