title: 3'UTR-dependent localization of a Purkinje cell messenger RNA in dendrites.
abstract: Pcp2(L7) is a Purkinje cell-specific GoLoco domain protein that modulates activation of Galphai/o proteins by G protein-coupled receptors. A likely downstream effector of this pathway is the P-type Ca(2+) channel, and thereby, the intrinsic electrophysiology of Purkinje cells could be modulated by Pcp2(L7). It has long been known that the Pcp2(L7) mRNA is abundantly localized in dendrites, suggesting the possibility of distal synthesis and local changes in levels of the protein. As a first step to uncover the trafficking and translational mechanisms for this mRNA, we have begun identifying the cis-acting sequences important for its localization in dendrites. Using expression of modified transgenes in vivo, we show that the 3'UTR, only 65 bases long, is necessary in this process.
authors.affiliation: Center for Molecular Neurobiology and Department of Neuroscience, The Ohio State University, 206 Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210, USA.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Xulun
authors.initials: X
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Bian
authors.forename: Xin-an
authors.initials: XA
authors.lastname: Pu
authors.forename: Karl
authors.initials: K
authors.lastname: Schilling
authors.forename: John
authors.initials: J
authors.lastname: Oberdick