A microRNA DNA methylation signature for human cancer metastasis.
MicroRNAs (miRNAs) are small, noncoding RNAs that can contribute to cancer development and progression by acting as oncogenes or tumor suppressor genes. Recent studies have also linked different sets of miRNAs to metastasis through either the promotion or suppression of this malignant process. Interestingly, epigenetic silencing of miRNAs with tumor suppressor features by CpG island hypermethylation is also emerging as a common hallmark of human tumors. Thus, we wondered whether there was a miRNA hypermethylation profile characteristic of human metastasis. We used a pharmacological and genomic approach to reveal this aberrant epigenetic silencing program by treating lymph node metastatic cancer cells with a DNA demethylating agent followed by hybridization to an expression microarray. Among the miRNAs that were reactivated upon drug treatment, miR-148a, miR-34b/c, and miR-9 were found to undergo specific hypermethylation-associated silencing in cancer cells compared with normal tissues. The reintroduction of miR-148a and miR-34b/c in cancer cells with epigenetic inactivation inhibited their motility, reduced tumor growth, and inhibited metastasis formation in xenograft models, with an associated down-regulation of the miRNA oncogenic target genes, such as C-MYC, E2F3, CDK6, and TGIF2. Most important, the involvement of miR-148a, miR-34b/c, and miR-9 hypermethylation in metastasis formation was also suggested in human primary malignancies (n = 207) because it was significantly associated with the appearance of lymph node metastasis. Our findings indicate that DNA methylation-associated silencing of tumor suppressor miRNAs contributes to the development of human cancer metastasis.
Lujambio
Amaia
A
Cancer Epigenetics Laboratory, Spanish National Cancer Research Center, Melchor Fernández Almagro 3, 28029 Madrid, Spain.
Calin
George A
GA
Villanueva
Alberto
A
Ropero
Santiago
S
Sánchez-Céspedes
Montserrat
M
Blanco
David
D
Montuenga
Luis M
LM
Rossi
Simona
S
Nicoloso
Milena S
MS
Faller
William J
WJ
Gallagher
William M
WM
Eccles
Suzanne A
SA
Croce
Carlo M
CM
Esteller
Manel
M