Study of binding glycyrrhetic acid to AT(1) receptor.
To analyze the binding of glycyrrhetic acid (GA) to angiotensin II type I (AT(1)) receptor and to explore the mechanisms underlying the binding, primary cell culture of rat vascular smooth muscle cell (VSMC), radioactive ligand-receptor binding assay, lascer confocal scanning microscope (LCSM), Northern blot, (3)H-TdR incorporation DNA assay were used in this study. The results suggest that specific binding of GA to AT(1) receptor (IC(50) value was 35.0 mumol/L) increases intracellular [Ca(2+)]i of VSMC, activates transcription factor c-myc and promotes the proliferation of VSMC, therefore GA was probably an agonist of AT(1) receptor, providing a new target for GA's pharmaceutical effects.
Zhang
Fengyun
F
Department of Biophysics, Health Science Center of Peking University, 100083, Beijing, China, fengyunzh@sohu.com.
Yue
Baozhen
B
He
Shipeng
S