title: Synthesis of DNA-organic molecule-DNA triblock oligomers using the amide coupling reaction and their enzymatic amplification.
abstract: Precise electrical contact between single-molecule and electrodes is a first step to study single-molecule electronics and its application such as (bio)sensors and nanodevices. To realize a reliable electrical contact, we can use DNA as a template in the field of nanoelectronics because of its micrometer-scaled length with the thickness of nanometer-scale. In this paper, we studied the reactivity of the amide-coupling reaction to tether oligodeoxynucleotides (ODNs) to organic molecules and the elongation of the ODNs by the polymerase chain reaction (PCR) to synthesize 1.5 kbp dsDNA-organic molecule-1.5 kbp dsDNA (DOD) triblock architecture. The successful amide-coupling reactions were confirmed by electrospray ionization mass spectrometry (ESI-MS), and the triblock architectures were characterized by 1% agarose gel electrophoresis and atomic force microscope (AFM). Our result shows that this strategy is simple and makes it easy to construct DNA-organic molecule-DNA triblock architectures and potentially provides a platform to prepare and investigate single molecule electronics.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California 94305, USA.
authors.forename: Jungkyu K
authors.initials: JK
authors.lastname: Lee
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Jung
authors.forename: Randall M
authors.initials: RM
authors.lastname: Stoltenberg
authors.forename: Jeffery B-H
authors.initials: JB
authors.lastname: Tok
authors.forename: Zhenan
authors.initials: Z
authors.lastname: Bao