Cloning and characterization of a novel member of human beta-1,4-galactosyltransferase gene family.
By using the EST strategy for identifying novel members belonging to homologous gene families, a novel fulklength cDNA encoding a protein significantly homologous to UDP-Gal: N-acetylglucosamine beta-1, 4-galactosyltransferase (GalT) was isolated from a human testis cDNA library. A nucleotide sequence of 2 173 bp long was determined to contain an open reading frame of 1 032 nucleotides (344 amino acids). In view of the homology to memben of the galactosyltransferase gene family and especially the closest relationship toGallus gallus GalT type I (CK I), the predicted product of the novel cDNA was designated as human beta-1,4-galactosyltransferase homolog I (HumGT-H1). Its mRNA is present in different degrees in 16 tissues examined. Southern analysis of human genomic DNA revealed its locus on chromosome 3.
Fan
Y
Y
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, 200433, Shanghai, China.
Yu
L
L
Zhang
Q
Q
Jiang
Y
Y
Dai
F
F
Chen
C
C
Tu
Q
Q
Bi
A
A
Xu
Y
Y
Zhao
S
S