title: Gas chromatographic separation of methoxylated polychlorinated biphenyl atropisomers.
abstract: Several polychlorinated biphenyls (PCBs) and their hydroxylated metabolites display axial chirality. Here we describe an enantioselective, gas chromatographic separation of methylated derivatives of hydroxylated (OH-)PCB atropisomers (MeO-PCB) using a chemically bonded beta-cyclodextrin column (Chirasil-Dex). The atropisomers of several MeO-PCBs could be separated on this column with resolutions ranging from 0.42 to 0.87 under isothermal or temperature-programmed conditions. In addition, the enantiomeric fraction of OH-PCB 136 metabolites was determined in male and female rats treated with racemic PCB 136. The methylated derivatives of two OH-PCB 136 metabolites showed an enantiomeric enrichment in liver tissue, whereas PCB 136 itself was near racemic.
authors.affiliation: Department of Occupational and Environmental Health, University of Iowa, 124 IREH, Iowa City, IA 52242, USA.
authors.forename: Izabela
authors.initials: I
authors.lastname: Kania-Korwel
authors.forename: Sandhya M
authors.initials: SM
authors.lastname: Vyas
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Lehmler