title: Expression and clinical significance of CacyBP/SIP in pancreatic cancer.
abstract: OBJECTIVE
  Calcyclin-binding protein or Siah-1-interacting protein (CacyBP/SIP), a component of the ubiquitin-mediated proteolysis, could bind SKP1-CUL1-F box protein complex and participate in beta-catenin degradation, which was found to be related to the malignant phenotypes of gastric cancer and renal cancer. However, the role of CacyBP/SIP in pancreatic cancer progression still remains unclear. Therefore, the aim of the present study was to investigate the expression and clinical significance of CacyBP/SIP in pancreatic cancer.
  
  
  METHODS
  Immunohistochemistry was carried out on paraffin-embedded sections of pancreatic cancer and normal pancreatic tissues. In addition, Western blot and semiquantitative RT-PCR were carried out to analyze mRNA and protein expression of CacyBP/SIP in 8 pairs of freshly resected pancreatic cancer and their adjacent nontumorous tissue.
  
  
  RESULTS
  CacyBP/SIP expression was significantly increased in pancreatic cancer tissue (28/68 or 41.2%) and correlated with differentiation degree, higher TNM (tumor, node, metastasis) stage and distance metastasis. Also, mRNA and protein expression of CacyBP/SIP were found to be at higher levels in almost all cancer tissues compared to adjacent tissues.
  
  
  CONCLUSIONS
  CacyBP/SIP protein might play an important role in the process of pancreatic carcinogenesis and high-level CacyBP/SIP expression might be related to the malignant potential of pancreatic cancer.
authors.affiliation: State Key Laboratory of Cancer Biology, Institute of Digestive Disease, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Chen
authors.forename: Guohong
authors.initials: G
authors.lastname: Han
authors.forename: Huihong
authors.initials: H
authors.lastname: Zhai
authors.forename: Faming
authors.initials: F
authors.lastname: Zhang
authors.forename: Jinbo
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Sheng
authors.initials: S
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan