Selecting aptamers for a glycoprotein through the incorporation of the boronic acid moiety.
The first general method for the selection of boronic acid-based aptamers (boronolectins) that allows for glycan substructure focusing is described. Using fibrinogen as a model glycoprotein, we have selected boronic acid-modified DNA aptamers that have high affinities (low nM Kd) and the ability to recognize changes in the glycosylation site. The method developed should also be applicable to the development of aptamers for other glycoproducts, such as glycolipids and glycopeptides.
Li
Minyong
M
Department of Chemistry and Center for Biotechnology and Drug Design, Georgia State University, Atlanta, Georgia 30302-4098, USA.
Lin
Na
N
Huang
Zhen
Z
Du
Lupei
L
Altier
Craig
C
Fang
Hao
H
Wang
Binghe
B