Sample preparation for liquid chromatography-tandem mass spectrometry using functionalized ferromagnetic micro-particles.
OBJECTIVE
To study the applicability of functionalized ferromagnetic micro-particles in the sample preparation for quantification of small molecules by LC-MS/MS.


METHODS
A representative analyte (itraconazole) was extracted from plasma samples using small quantities of C18-modified ferromagnetic micro-particles.


RESULTS
125 microg of functionalized micro-particles was sufficient to extract the target analyte from 10 microL of plasma with a recovery rate of approximately 100%; linearity (r>0.99) and reproducibility (inter-assay CV< or =7%) of quantitative results was found.


CONCLUSIONS
C18-functionalized ferromagnetic particles can be used to efficiently extract small molecule analytes from complex biological matrices for quantitative analyses using LC-MS/MS.
Vogeser
Michael
M
Institute of Clinical Chemistry, Hospital of the University of Munich, Marchioninistr. 15, D-81377 Munich, Germany. Michael.Vogeser@med.uni-muenchen.de
Geiger
Albert
A
Herrmann
Rupert
R
Kobold
Uwe
U