title: Expression of recombinant human nerve growth factor beta in milk of goats by recombinant replication-defective adenovirus.
abstract: For producing a large quantity of human nerve growth factor beta (hNGF-beta), the recombinant adenovirus containing an expression cassette of hNGF-beta complementary DNA gene was constructed and then instilled directly into goats' mammary glands. The recombinant hNGF-beta was detected in the milk. The maximal expression level of recombinant hNGF-beta in the milk reached 196.8 mg/l. The biological activity of the recombinant hNGF-beta, compared with the commercial hNGF-beta, was tested using PC12 cells. The results demonstrate that instilling recombinant adenovirus directly into the mammary gland of goat is an efficient approach to producing a large quantity of hNGF-beta.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
authors.forename: Qingwang
authors.initials: Q
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Zengsheng
authors.initials: Z
authors.lastname: Han
authors.forename: Dawei
authors.initials: D
authors.lastname: Gao
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Xubo
authors.initials: X
authors.lastname: Zhi
authors.forename: Hai
authors.initials: H
authors.lastname: Yang
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Liu