[Effect of compound Puerarin on the collage IV in streptozotocin-induced diabetic nephropathy rats].
OBJECTIVE
To observe the effect of compound Puerarin on collagen IV of streptozotocin-induced diabetic rats.


METHODS
Diabetic nephropathy rats were induced by intraperitoneal injection of streptozotocin (STZ). Rats were allocated randomly to control group (10), diabetes model group (10), Vitamin C group (10), Puerarin group (10), vitamin C plus Puerarin group (10). The study period lasted for 12 weeks. During and after the treatment, the general state, blood glucose levels, glycosylated hemoglobin, blood urea nitrogen, serum collagen IV, blood urea nitrogen, serum creatinine, urinary albumin excretion rate of the 24-hour, and clearance rate of creatinine collagen IV protein were determined by immunohistochemistoche analysis as well as type the gene expression of collagen IV alpha 1 mRNA were determined by in situ hybridization analysis in the kidney tissue of different groups.


RESULTS
(1) Diabetes mellitus and renal function lesion occurred in the four groups. (2) Vitamin C and Puerarin could improve the general conditions of diabetic Rats, decrease blood urea nitrogen [(8.68 +/- 0.43), (7.98 +/- 0.47) and (5.76 +/- 0.82) micromol/L, serum creatinine [(74.68 +/- 8.20), (75.52 +/- 7.98) and (58.66 +/- 6.65) mmol/L], and urinary albumin excretion rate of the 24-hour [(18.40 +/- 0.37), (17.24 +/- 0.30) and (9.97 +/- 1.27) mg/24 h x 10(-3)]; increase clearance rate of creatinine [(0.59 +/- 0.21), (0.61 +/- 0.14) and (0.69 +/- 0.32) ml/min], the expression of collage IV absorbance [(111.56 +/- 14.61), (110.78 +/- 9.69) and (95.44 +/- 9.97) ] in the diabetic Rats were significantly inhibited at the same time.


CONCLUSIONS
The compound Puerarin might have some functions on preventing ren by inhibiting expression of type IV collagen.
Li
Qiang-xiang
QX
Department of Endocrinology, Loudi Municipal Central Hospital of Hunan Province, Loudi 417000, China.
Zhong
Hui-ju
HJ
Gong
Han-ren
HR
Zhu
Fei-yue
FY
Wang
Lin-na
LN
Shen
Dao-jun
DJ
Li
Guo
G
Wang
Cai-yun
CY
Qin
Cheng-sheng
CS