title: Petrobactin biosynthesis: AsbB catalyzes condensation of spermidine with N8-citryl-spermidine and its N1-(3,4-dihydroxybenzoyl) derivative.
abstract: The AsbB enzyme, which is involved in the biosynthesis of the virulence-conferring siderophore petrobactin in Bacillus anthracis, is shown to catalyze efficient ATP-dependent condensation of spermidine, but not N1-(3,4-dihydroxbenzoyl)-spermidine, with N8-citryl-spermidine or N1-(3,4-dihydroxbenzoyl)-N8-citryl-spermidine, suggesting that N1-(3,4-dihydroxbenzoyl)-spermidine is very unlikely to be a significant intermediate in petrobactin biosynthesis, contrary to previous suggestions.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, UK CV4 7AL.
authors.forename: Daniel
authors.initials: D
authors.lastname: Oves-Costales
authors.forename: Nadia
authors.initials: N
authors.lastname: Kadi
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Fogg
authors.forename: Lijiang
authors.initials: L
authors.lastname: Song
authors.forename: Keith S
authors.initials: KS
authors.lastname: Wilson
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Challis