title: In-capillary derivatization and capillary electrophoresis separation of amino acid neurotransmitters from brain microdialysis samples.
abstract: A new in-capillary derivatization method with naphtalene-2,3-dicarboxyaldehyde (NDA)/CN(-) has been developed for capillary electrophoresis with laser-induced fluorescence detection of brain microdialysate amino acids. Samples are sandwiched between two plugs of reagent mixture at the capillary inlet and subsequently separated. Highest derivatization yields are obtained by using a reagent to sample plug length ratio equal to 4, performing a first electrophoretic mixing followed by a zero potential amplification step before applying the separation voltage and using a NaCN to NDA concentration ratio equal to 1. This new single-step methodology allows the analysis of amino acid neurotransmitters in rat brain microdialysis samples.
authors.affiliation: Université de Lyon, F-69008 Lyon, France. luc.denoroy@recherche.univ-lyon1.fr
authors.forename: Luc
authors.initials: L
authors.lastname: Denoroy
authors.forename: Sandrine
authors.initials: S
authors.lastname: Parrot
authors.forename: Louis
authors.initials: L
authors.lastname: Renaud
authors.forename: Bernard
authors.initials: B
authors.lastname: Renaud
authors.forename: Luc
authors.initials: L
authors.lastname: Zimmer