title: Study of binding glycyrrhetic acid to AT(1) receptor.
abstract: To analyze the binding of glycyrrhetic acid (GA) to angiotensin II type I (AT(1)) receptor and to explore the mechanisms underlying the binding, primary cell culture of rat vascular smooth muscle cell (VSMC), radioactive ligand-receptor binding assay, lascer confocal scanning microscope (LCSM), Northern blot, (3)H-TdR incorporation DNA assay were used in this study. The results suggest that specific binding of GA to AT(1) receptor (IC(50) value was 35.0 mumol/L) increases intracellular [Ca(2+)]i of VSMC, activates transcription factor c-myc and promotes the proliferation of VSMC, therefore GA was probably an agonist of AT(1) receptor, providing a new target for GA's pharmaceutical effects.
authors.affiliation: Department of Biophysics, Health Science Center of Peking University, 100083, Beijing, China, fengyunzh@sohu.com.
authors.forename: Fengyun
authors.initials: F
authors.lastname: Zhang
authors.forename: Baozhen
authors.initials: B
authors.lastname: Yue
authors.forename: Shipeng
authors.initials: S
authors.lastname: He