title: Circadian variation of rectal sensitivity and gastrointestinal peptides in healthy volunteers.
abstract: The aim of our study was to identify diurnal variation of perception of rectal distension and the release of gastroenteropancreatic hormones. In 12 healthy male volunteers (25 years, range 22-32), a rectal balloon distension was performed. Rectal perception thresholds (minimal, urge and pain) and rectal compliance were double-measured with a computer-controlled barostat at seven standardized time points during the day (from 16.00 to 14.00 hours the following day). Blood samples were taken 30 min before and after each rectal distension procedure to determine plasma levels of cholecystokinin (CCK), pancreatic polypeptide (PP) and motilin. Sensory thresholds for urge and pain varied significantly with the time of day, with higher threshold levels in the evening than in the morning hours. Bowel wall compliance showed as well-significant variance at pain threshold and was higher during daytime than in the evening or at night. In contrast to motilin, release of CCK and PP also showed a significant variation depending on daytime. Perception of rectal distension stimuli as well as compliance was independent of intake of food and peptide hormone levels, but CCK and PP levels increased with food, and PP levels decreased with rectal distension. Significant differences in the perception of rectal distension stimuli for urge and pain depending on daytime were found, but the release of gastrointestinal peptides seemed not to be involved. This circadian variation needs to be taken into account in patients and volunteer studies.
authors.affiliation: Medical Department VI, University Hospital Tübingen, Tübingen, Germany. paul.enck@uni-tuebingen.de
authors.forename: P
authors.initials: P
authors.lastname: Enck
authors.forename: C
authors.initials: C
authors.lastname: Kaiser
authors.forename: M
authors.initials: M
authors.lastname: Felber
authors.forename: R L
authors.initials: RL
authors.lastname: Riepl
authors.forename: A
authors.initials: A
authors.lastname: Klauser
authors.forename: S
authors.initials: S
authors.lastname: Klosterhalfen
authors.forename: B
authors.initials: B
authors.lastname: Otto