title: Impact of gag mutations on selection of darunavir resistance mutations in HIV-1 protease.
abstract: OBJECTIVE
  To search for genetic factors in the protease and gag regions (NC-p1/TFP-p6/p6pol) involved in selection of darunavir resistance mutations.
  
  
  METHODS
  We analysed 48 protease inhibitor (PI)-experienced HIV-infected patients experiencing darunavir treatment failure. Viral genotyping at baseline and months 3 and 6 was used to assess the selection of mutations in the protease and gag regions conferring resistance to PIs.
  
  
  RESULTS
  There were no genotypic differences in the studied gag region between baseline and the latest available rebound isolates. There was an association between the presence of the mutation A431V in the gag sequence and the selection of the L76V mutation in the protease sequence in the latest available rebound. The I437T/V mutation in gag and the L76V mutation in the protease were associated with a lower risk of selecting darunavir resistance mutations.
  
  
  CONCLUSIONS
  In these PI-treated patients experiencing treatment failure of a darunavir-containing regimen, we showed that mutations in the gag region NC-p1/TFP-p6/p6pol may influence the selection of darunavir resistance mutations; in particular, the I437T/V gag mutation that confers resistance to PIs reduces the selection of such mutations. Virus with L76V in protease or I437T/V in gag may be already resistant to darunavir and, therefore, no additional resistance mutations need to be selected.
authors.affiliation: UPMC Univ Paris 06, EA 2387, F-75005, Paris, France. sidonie.lambert@psl.aphp.fr
authors.forename: Sidonie
authors.initials: S
authors.lastname: Lambert-Niclot
authors.forename: Philippe
authors.initials: P
authors.lastname: Flandre
authors.forename: Isabelle
authors.initials: I
authors.lastname: Malet
authors.forename: Ana
authors.initials: A
authors.lastname: Canestri
authors.forename: Cathia
authors.initials: C
authors.lastname: Soulié
authors.forename: Roland
authors.initials: R
authors.lastname: Tubiana
authors.forename: Christel
authors.initials: C
authors.lastname: Brunet
authors.forename: Marc
authors.initials: M
authors.lastname: Wirden
authors.forename: Christine
authors.initials: C
authors.lastname: Katlama
authors.forename: Vincent
authors.initials: V
authors.lastname: Calvez
authors.forename: Anne-Geneviève
authors.initials: AG
authors.lastname: Marcelin