title: Genetic mapping of a new semi-dwarf gene, sd-t(t), in indica rice and estimating of the physical distance of the mapping region.
abstract: Application and functional study of dwarf and semi-dwarf genes are of great importance to both crop breeding and molecular biology. A new semi-dwarf gene, sd-t(t), non-allelic to sd-1, had been identified in an indica rice variety, Aitaiyin 2. In this study the gene was genetically mapped by using an F(2) population, which consisted of 474 individuals developed from a cross between Aitaiyin 2 and B30. The sd-t(t) gene was located between the RFLP markers R514 and R1408B with a distance of 1.1 cM to R514, and 4.5 cM to R1408B on chromosome 4. A physical contig covering the sd-t(t) mapping region was further constructed by screening a BAC library with R514 and R1408B as probes, and the physical distance between R514 and R1408B was estimated at approximately 147 kb. This result will facilitate map-based cloning of the sd-t(t) gene.
authors.affiliation: School of Life Science, University of Science and Technology of China, 230026, Hefei, China.
authors.forename: Guanghuai
authors.initials: G
authors.lastname: Jiang
authors.forename: Guohua
authors.initials: G
authors.lastname: Liang
authors.forename: Wenxue
authors.initials: W
authors.lastname: Zhai
authors.forename: Minghong
authors.initials: M
authors.lastname: Gu
authors.forename: Runlong
authors.initials: R
authors.lastname: Lu
authors.forename: Jichen
authors.initials: J
authors.lastname: Xu
authors.forename: Lihuang
authors.initials: L
authors.lastname: Zhu