Intestinal gluconeogenesis is a key factor for early metabolic changes after gastric bypass but not after gastric lap-band in mice.
Unlike the adjustable gastric banding procedure (AGB), Roux-en-Y gastric bypass surgery (RYGBP) in humans has an intriguing effect: a rapid and substantial control of type 2 diabetes mellitus (T2DM). We performed gastric lap-band (GLB) and entero-gastro anastomosis (EGA) procedures in C57Bl6 mice that were fed a high-fat diet. The EGA procedure specifically reduced food intake and increased insulin sensitivity as measured by endogenous glucose production. Intestinal gluconeogenesis increased after the EGA procedure, but not after gastric banding. All EGA effects were abolished in GLUT-2 knockout mice and in mice with portal vein denervation. We thus provide mechanistic evidence that the beneficial effects of the EGA procedure on food intake and glucose homeostasis involve intestinal gluconeogenesis and its detection via a GLUT-2 and hepatoportal sensor pathway.
Troy
Stephanie
S
Institut National de la Sante et de la Recherche Medicale, U695, Faculté de Médecine Xavier Bichat, Universite Paris 7, Paris, F-75870, France.
Soty
Maud
M
Ribeiro
Lara
L
Laval
Laure
L
Migrenne
Stéphanie
S
Fioramonti
Xavier
X
Pillot
Bruno
B
Fauveau
Veronique
V
Aubert
Roberte
R
Viollet
Benoit
B
Foretz
Marc
M
Leclerc
Jocelyne
J
Duchampt
Adeline
A
Zitoun
Carine
C
Thorens
Bernard
B
Magnan
Christophe
C
Mithieux
Gilles
G
Andreelli
Fabrizio
F