title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of hypothetical protein SCO4226 from Streptomyces coelicolor A3(2).
abstract: A non-Pfam hypothetical protein SCO4226 of molecular weight 9 kDa from Streptomyces coelicolor A3(2) was overexpressed in Escherichia coli and the purified recombinant protein was crystallized using the sitting-drop vapour-diffusion method. An X-ray diffraction data set was collected to 2.0 A resolution. The crystal belonged to space group P2(1), with unit-cell parameters a = 29.67, b = 67.00, c = 34.43 A, alpha = gamma = 90.00, beta = 94.26 degrees .
authors.affiliation: School of Life Sciences and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, People's Republic of China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Yong Xing
authors.initials: YX
authors.lastname: He
authors.forename: Rui
authors.initials: R
authors.lastname: Bao
authors.forename: Yan Bin
authors.initials: YB
authors.lastname: Teng
authors.forename: Bo Ping
authors.initials: BP
authors.lastname: Ye
authors.forename: Cong Zhao
authors.initials: CZ
authors.lastname: Zhou