title: Effect of biofilm formation on virulence factor secretion via the general secretory pathway in Streptococcus mutans.
abstract: OBJECTIVE
  To investigate the role of SecA in protein secretion, and to evaluate the effect of biofilm formation on protein secretion in Streptococcus mutans.
  
  
  METHODS
  S. mutans strains UA159 and GS-5 were used in this study. Cells grown in biofilm and planktonic conditions were observed using immunogold electron microscopy. The mRNA levels of ftf, gtfB, gtfC, gtfD, Pac and secA were analysed in different growth conditions using real-time quantitative polymerase chain reaction. The levels of wall proteins and whole-cell protein extracts were examined using Western blot analysis.
  
  
  RESULTS
  A microdomain colocalised with SecA and virulence factors such as Pac (AgI/II) and glucosyltransferase (GTF) was observed. The mRNA level of secA was upregulated in the biofilm condition. The level of protein expression of SecA and wall protein levels of GTF, fructosyltransferase (FTF) and Pac (AgI/II) in the biofilm condition were significantly higher than in the planktonic condition.
  
  
  CONCLUSIONS
  These data suggest that S. mutans utilises the Sec pathway to secrete virulence factor proteins such as Pac (AgI/II), GTF and FTF, and protein secretion occurred at a distinct microdomain. The level of SecA, the key factor in the Sec pathway, was influenced significantly by biofilm formation in S. mutans.
authors.affiliation: Key Laboratory for Oral Biomedical Engineering Ministry of Education, School and Hospital of Stomatology, Wuhan University, Luoyu Road 237, 430079 Wuhan, Hubei, PR China.
authors.forename: MeiJing
authors.initials: M
authors.lastname: Huang
authors.forename: LiuYan
authors.initials: L
authors.lastname: Meng
authors.forename: MingWen
authors.initials: M
authors.lastname: Fan
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Bian