TaqMan-based real-time PCR method for detection of Yersinia pseudotuberculosis in food.
A sensitive and specific assay for detection of food-borne pathogenic Yersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located gene ail. The complete method, including an internal amplification control, was evaluated for several different food items.
Lambertz
S Thisted
ST
Research and Development Department, National Food Administration, Uppsala, Sweden.
Nilsson
C
C
Hallanvuo
S
S