title: Efficient ends-out gene targeting in Drosophila.
abstract: In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-positive targeting candidates.
authors.affiliation: Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennyslvania 15261, USA.
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.forename: Wenke
authors.initials: W
authors.lastname: Zhou
authors.forename: Annie M
authors.initials: AM
authors.lastname: Watson
authors.forename: Yuh-Nung
authors.initials: YN
authors.lastname: Jan
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong