Crystallization and X-ray analysis of the Schistosoma mansoni guanidino kinase.
The 716-amino-acid guanidino kinase from the parasitic flatworm Schistosoma mansoni results from the fusion of two guanidino kinase subunits. Crystals of this 80 kDa protein have been obtained in the monoclinic space group P2(1), with unit-cell parameters a = 52.7, b = 122.1, c = 63.2 A, beta = 108.5 degrees . Synchrotron data were collected to 2.8 A resolution on ESRF beamline ID29. The structure was solved by the molecular-replacement method, using the 357-amino-acid structure of the arginine kinase from Trypanosoma cruzi as the search model.
Awama
Ayman M
AM
Institut de Chimie et Biochimie Moléculaire et Supramoléculaire, CNRS UMR 5246 et Université de Lyon, F-69622 Villeurbanne, France.
Paracuellos
Patricia
P
Laurent
Sabine
S
Dissous
Colette
C
Marcillat
Olivier
O
Gouet
Patrice
P