The application of CdTe@SiO2 particles in immunoassay.
CdTe@SiO(2) fluorescent particles were synthesized via hydrolysis and condensation of tetraethyl orthosilicate (TEOS) in water-in-oil (W/O) emulsion. Uniform luminophore-doped silica nanoparticles with 100nm in diameter were obtained using microemulsion method and characterized by SEM. Antibody proteins were successfully conjugated to the fluorescent particles by the reaction of avidin and biotin, which were confirmed by fluorescence spectra. CdTe@SiO(2) fluorescent particles were potentially useful for the applications in biolabeling and imaging.
Jin
Li
L
Department of Analytical Chemistry, College of Chemistry, Jilin University, Jiefang Road 2519, Changchun 130023, China.
Yu
Dong-Dong
DD
Liu
Yan
Y
Zhao
Xiao-Li
XL
Zhou
Jian-Guang
JG