title: Ki-67 expression predicts locoregional recurrence in stage I oral tongue carcinoma.
abstract: Oral tongue squamous cell carcinoma (OTSCC) is an aggressive cancer associated with poor prognosis. Methods for determining the aggressiveness of OTSCC from analysis of the primary tumour specimen are thus highly desirable. We investigated whether genomic instability and proliferative activity (by means of Ki-67 activity) could be of clinical use for prediction of locoregional recurrence in 76 pretreatment OTSCC paraffin samples (stage I, n=22; stage II, n=33; stage III, n=8; stage IV, n=13). Eleven surgical tumour specimens were also analysed for remnants of proliferative activity after preoperative radiotherapy. Ninety-seven percent of cases (n=72) were characterised as being aneuploid as measured by means of image cytometry. Preoperative radiotherapy (50-68 Gy) resulted in significant reduction of proliferative activity in all patients for which post-treatment biopsies were available (P-value=0.001). Proliferative activity was not associated with response to radiation in stage II patients. However, we report a significant correlation between high proliferation rates and locoregional recurrences in stage I OTSCC patients (P-value=0.028). High-proliferative activity is thus related to an elevated risk of recurrence after surgery alone. We therefore conclude that Ki-67 expression level is a potentially useful clinical marker for predicting recurrence in surgically treated stage I OTSCC.
authors.affiliation: Department of Oncology-Pathology, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden. darawalee.wangsa@ki.se
authors.forename: D
authors.initials: D
authors.lastname: Wangsa
authors.forename: M
authors.initials: M
authors.lastname: Ryott
authors.forename: E
authors.initials: E
authors.lastname: Avall-Lundqvist
authors.forename: F
authors.initials: F
authors.lastname: Petersson
authors.forename: G
authors.initials: G
authors.lastname: Elmberger
authors.forename: J
authors.initials: J
authors.lastname: Luo
authors.forename: T
authors.initials: T
authors.lastname: Ried
authors.forename: G
authors.initials: G
authors.lastname: Auer
authors.forename: E
authors.initials: E
authors.lastname: Munck-Wikland