title: The centromeric satellite of the wedge sole (Dicologoglossa cuneata, Pleuronectiformes) is composed mainly of a sequence motif conserved in other vertebrate centromeric DNAs.
abstract: Here, a new satellite-DNA family is isolated and characterized from wedge sole, Dicologoglossa cuneata Moreau, 1881 (Pleuronectiformes), a fish having a small genome. This satellite-DNA family of sequences was isolated by conventional cloning after digestion of genomic DNA with the DraI restriction enzyme. Repeat units are 171 bp in length with a high AT content (63%). Several runs of consecutive adenines and thymines were found, and concomitantly computer analyses revealed that these regions are prone to acquire stable sequence-directed curvature. Especially remarkable is that the DraI sequences are composed almost entirely of the repetition of up to fourteen 9-bp motifs (T/C)GTC(A/C)AAAA similar to other vertebrate centromeric satellite-DNA sequences. In fact, we demonstrate the origin of this satellite through duplication of this motif plus the addition of a stretch of cytosines. The centromeric location and the presence in this satellite-DNA sequence of not only different vertebrate motifs (CENP-B box, pJalpha) but also others such as the CDEIII motif of Saccharomyces cerevisiae reveal a possible role in centromere function. All these characteristics provide important information on the origin, function, and the evolution of the centromeric satellite DNAs in wedge sole.
authors.affiliation: Departamento de Genética, Facultad de Ciencias, Universidad de Granada, Granada, Spain. rherran@ugr.es
authors.forename: R
authors.initials: R
authors.lastname: de la Herrán
authors.forename: F
authors.initials: F
authors.lastname: Robles
authors.forename: J I
authors.initials: JI
authors.lastname: Navas
authors.forename: I
authors.initials: I
authors.lastname: López-Flores
authors.forename: M
authors.initials: M
authors.lastname: Herrera
authors.forename: I
authors.initials: I
authors.lastname: Hachero
authors.forename: M A
authors.initials: MA
authors.lastname: Garrido-Ramos
authors.forename: C
authors.initials: C
authors.lastname: Ruiz Rejón
authors.forename: M
authors.initials: M
authors.lastname: Ruiz Rejón