Description of Ancylobacter oerskovii sp. nov. and two additional strains of Ancylobacter polymorphus.
A Gram-negative, pleomorphic, rod-shaped, non-spore-forming bacterium, designated strain NS05T, was isolated from soil after enrichment with oxalate. On the basis of 16S rRNA gene sequence similarity, strain NS05T was shown to be phylogenetically related to the genera Ancylobacter, Starkeya and Angulomicrobium (96.3-98.1% sequence similarity), class Alphaproteobacteria. Strain NS05T was most closely related to Ancylobacter rudongensis AS 1.1761T (98.1% sequence similarity). The whole-cell fatty acid pattern of strain NS05T was typical of those found in members of the genus Ancylobacter. Its main components were C(18:1)omega7c (60.4%), C(19:0)omega8c cyclo (28.3%) and C(16:0) (7.4%) and hydroxylated compounds were absent. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain NS05T with respect to the four Ancylobacter species with validly published names. Therefore, it is concluded that NS05T represents a novel species of the genus Ancylobacter, for which the name Ancylobacter oerskovii sp. nov. is proposed. The type strain is NS05T (=DSM 18746T=CCM 7435T). Two other oxalate-utilizing strains, NS03 and NS04, isolated from paper-mill effluents, were shown by 16S rRNA gene sequence analysis to be affiliated to the species Ancylobacter polymorphus. The study of their physiological properties extends the knowledge of the physiological variability within this species.
Lang
Elke
E
Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-30124 Braunschweig, Germany. ela@dsmz.de
Swiderski
Jolantha
J
Stackebrandt
Erko
E
Schumann
Peter
P
Spröer
Cathrin
C
Sahin
Nurettin
N