Glutamine deprivation causes enhanced plating efficiency of a herpes simplex virus type 1 ICP0-null mutant.
Isoleucine deprivation of cellular monolayers prior to infection has been reported to result in partial complementation of a herpes simplex virus type 1 (HSV-1) ICP0 null (ICP0(-)) mutant. We now report that glutamine deprivation alone is able to enhance the plating efficiency of an ICP0(-) virus and that isoleucine deprivation has little or no effect. Because a low glutamine level is associated with stress and because stress is known to induce reactivation, low levels of glutamine may be relevant to the reactivation of HSV-1 from latency. Additionally, we demonstrate that arginine and methionine deprivation result in partial complementation of the ICP0(-) virus.
Bringhurst
Ryan M
RM
University of Arizona, 1007 E. Lowell St., P.O. Box 210106, Tucson, AZ 85721, USA. rbring@email.arizona.edu
Dominguez
Antonia A
AA
Schaffer
Priscilla A
PA