Diagnosis of lymphoproliferative disease virus infection of turkeys by an indirect immunofluorescence test.
An indirect immunofluorescence (IIF) test for the detection of lympho-proliferative disease virus (LPDV) is described. Results presented show that smears of buffy coat cells and frozen sections of spleen, but not thymus, form suitable test materials for a rapid and specific diagnosis of LPDV infection of turkeys. Between 0.1 and 0.6% of buffy coat cells from infected birds were antigen positive, and spleens from 18/18 and thymuses from 11/18 birds respectively, tested 16 months after viral inoculation, were positive. Antisera containing group-specific antibodies to avian reticuloendotheliosis virus (REV) and avian sarcoma-leukosis virus (ASLV) did not cross-react with LPDV-infected buffy coat cells or spleens.
Patel
J R
JR
Houghton Poultry Research Station, Houghton, Huntingdon, Cambridgeshire, England.
Shilleto
R W
RW