Analysis of green pit viper (Trimeresurus alborabris) venom protein by LC/MS-MS.
Green pit viper venom has major effect on the hematological system having a thrombin-like effect. Thus, this study is designed to analyze the composition of Trimeresurus albolabris venom by performing gel filtration and LC/MS-MS. The purified protein was then digested by trypsin, and the tryptic fragments were analyzed by iontrap spectrophotometry. This study found four types of proteins, namely jerdonitin, stejaggregin-A beta chain-1, stejnobin, and stejnihagin-A, as the components of T. albolabris venom. All of these toxins played a greater or lesser role in clot formation or otherwise contributed to cross-reactions in antivenom production.
Soogarun
Suphan
S
Clinical Microscopy Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Sangvanich
Polkit
P
Chowbumroongkait
Montri
M
Jiemsup
Surasak
S
Wiwanikit
Viroj
V
Pradniwat
Paweena
P
Palasuwan
Attakorn
A
Pawinwongchai
Jaturawat
J
Chanprasert
Supanthitra
S
Moungkote
Tusrin
T