Efficient ends-out gene targeting in Drosophila.
In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-positive targeting candidates.
Huang
Juan
J
Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennyslvania 15261, USA.
Zhou
Wenke
W
Watson
Annie M
AM
Jan
Yuh-Nung
YN
Hong
Yang
Y