title: Isolation of development-related genes in somatic embryo radicle of carrot (Daucus carota L.) using modified cDNA RDA.
abstract: Using modified cDNA RDA capitalizing on the high affinity of streptavidin for biotin and magnetic-absorption-based separation, we have obtained four bands of specifically expressed cDNA in the carrot somatic embryo deregulated for 12 h, which were designated as NR-1, NR-2, NR-3 and NR-4, respectively. As revealed by homology analysis of their DNA sequences after cloning them into pBS, remarkable homology was demonstrated in NR-2, NR-3 and NR-4 with the genes coding for LEA (late embryogenesis abundant protein), Dna J and xyloglucan endo-transglycosylase in plants. On the other hand, NR-1 showing no homology with any known sequence may have come from unknown genes. Using(32)P-labeled NR-1 as probe, hybridization with cDNA fragment population has shown that we have actually cloned a new gene fragment related to radicle development. As shown by further Southern hybridization, these genes may be present in carrot genome in the form of single or low copies.
authors.affiliation: Institute of Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: F
authors.initials: F
authors.lastname: Diao
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Huang
authors.forename: N
authors.initials: N
authors.lastname: Wu