title: Enhanced expression of laccase during the degradation of endocrine disrupting chemicals in Trametes versicolor.
abstract: A putative laccase cDNA from a white-rot basidiomycete, Trametes versicolor, that consisted of 1,769 nucleotides was cloned using the rapid amplification of cDNA ends (RACE)-PCR method. The deduced amino acid sequence had 4 putative copper binding regions, which are common to fungal laccases. In addition, the sequence was 57-97% homologous to sequences of other T. versicolor laccases. Additionally, the expression of laccase and manganese peroxidase in this fungus were both greatly increased under degrading conditions for bisphenol A, nonylphenol and two phthalic esters (benzylbutylphthalate and diethylphthalate), all of which are reportedly endocrine disrupting chemicals (EDCs). Furthermore, the estrogenic activities of the EDCs also decreased rapidly during incubation when examined in a two-hybrid yeast system. Finally, kojic acid inhibited the removal of estrogenic activities generated by bisphenol A and nonylphenol, which confirmed that laccase was involved in the degradation of EDCs in T. versicolor.
authors.affiliation: Division of Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Yunjung
authors.initials: Y
authors.lastname: Kim
authors.forename: Sumin
authors.initials: S
authors.lastname: Yeo
authors.forename: Hong-Gyu
authors.initials: HG
authors.lastname: Song
authors.forename: Hyoung T
authors.initials: HT
authors.lastname: Choi