Preparation and application of monoclonal antibody against hNDRG2.
The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5alpha as a 6x His-tagged protein. The purified 6x His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.
Liu
Xinping
X
Department of Biochemistry and Molecular Biology, The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
Hu
Xiaolan
X
Zhang
Jian
J
Wang
Lifeng
L
Zhang
Wenghong
W
Liu
Xuesong
X
Li
Fuyang
F
Zhang
Yuanqiang
Y
Yao
Libo
L