title: Restoration of FGF receptor type 2 enhances radiosensitivity of hormone-refractory human prostate carcinoma PC-3 cells.
abstract: BACKGROUND
  This study was undertaken to investigate the radiosensitizing effects of fibroblast growth factor receptor 2IIIb (FGFR2IIIb) in androgen-independent human prostate carcinoma PC-3 cells devoid of normally resident epithelial cell FGFR2IIIb.
  
  
  METHODS
  A clonal line of PC-3 cells expressing FGFR2IIIb was established by stable transfection. Clonogenic cell survival, apoptosis and cell cycle distribution with and without gamma-irradiation were then compared between FGFR2IIIb-expressing PC-3 cells and control cells mock-transfected with vector alone.
  
  
  RESULTS
  Gamma-irradiation resulted in an increase of clonogenic cell death concurrent with enhanced apoptosis and cell cycle arrest in the G2/M-phase in both transfected and untransfected cells. A quantitative analysis of all three parameters indicated that cells expressing FGFR2IIIb were significantly more sensitive to irradiation than control cells.
  
  
  CONCLUSIONS
  These results indicate that restoration of FGFR2IIIb to PC-3 cells enhances their sensitivity to irradiation through acceleration of apoptosis and cell cycle arrest.
authors.affiliation: Department of Urology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. matsua@hiroshima-u.ac.jp
authors.forename: Akio
authors.initials: A
authors.lastname: Matsubara
authors.forename: Jun
authors.initials: J
authors.lastname: Teishima
authors.forename: Suichinov
authors.initials: S
authors.lastname: Mirkhat
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yasumoto
authors.forename: Hideki
authors.initials: H
authors.lastname: Mochizuki
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Seki
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Mutaguchi
authors.forename: Wallace L
authors.initials: WL
authors.lastname: Mckeehan
authors.forename: Tsuguru
authors.initials: T
authors.lastname: Usui