title: Comparison of Escherichia coli uropathogenic genes (kps, usp and ireA) and enteroaggregative genes (aggR and aap) via multiplex polymerase chain reaction from suprapubic urine specimens of young children with fever.
abstract: OBJECTIVE
  Escherichia coli is the most frequently identified microbiological agent in childhood urinary tract infections (UTIs). However, the pathogenic role of this organism in young children remains to be clearly elucidated. So far, no studies have been conducted in which multiplex polymerase chain reaction (PCR) has been applied to determine the association between childhood UTIs and E. coli and urovirulent genes.
  
  
  METHODS
  Altogether, 330 suprapubic urine specimens from febrile young children were cultured. In 33 of the cases, E. coli was identified; among these cases, 18 had a UTI (>10(4)-10(5) cfu/ml), four had a suspected UTI (>10(2)-10(3) cfu/ml) and 11 did not have UTIs (10(2) cfu/ml). Using multiplex PCR, three uropathogenic E. coli (UPEC) genes and two enteroaggregative E. coli (EAEC) genes were detected.
  
  
  RESULTS
  In the UTI-UPEC cases, the kps gene was detected in 18 of 22 cases (82%) and the usp gene in 16 of 22 cases (73%). Among the 18 cases of children with UTIs characterized by 10(4)-10(5) E. coli cfu/ml, urinary tract abnormalities were identified via dimercaptosuccinic acid scans in seven of 18 cases (39%) and via voiding cystourethrograms in four of the 18 cases (22%).
  
  
  CONCLUSIONS
  The UPEC kps and usp genes were clearly associated with childhood UTIs, and may also be associated with kidney or urinary tract dysfunctions in young children. Escherichia coli colony count numbers in excess of 10(4)-10(5) cfu/ml in the suprapubic urine were considered to be strong evidence of UTI in infants.
authors.affiliation: Departments of Microbiology, Gachon University of Medicine and Science, Incheon, South Korea. parkhk@ewha.ac.kr
authors.forename: Hae-Kyung
authors.initials: HK
authors.lastname: Park
authors.forename: Yun-Jae
authors.initials: YJ
authors.lastname: Jung
authors.forename: Hee-Chung
authors.initials: HC
authors.lastname: Chae
authors.forename: Yoo-Jung
authors.initials: YJ
authors.lastname: Shin
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Woo
authors.forename: Hye-Sook
authors.initials: HS
authors.lastname: Park
authors.forename: Seung-Joo
authors.initials: SJ
authors.lastname: Lee