Large-scale recordings for drug screening in neural circuit systems.
Functional multineuron calcium imaging (fMCI) is a large-scale optical technique that records the suprathreshold activity from large neuron populations. fMCI has several advantages, including: i) simultaneous recording from hundreds of neurons, ii) single-cell resolution, iii) identifiable location of neurons, and iv) detection of non-active neurons during the observation period. I review the principle and detailed method of fMCI and also describe the effect of oseltamivir on neuronal network as an example for practical application of fMCI.
Ikegaya
Yuji
Y
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan. ikegaya@mol.f.u-tokyo.ac.jp