7-hydroxycoumarin-hemicyanine hybrids: a new class of far-red emitting fluorogenic dyes.
The design and synthesis of novel water-soluble far-red emitting phenol-based fluorophores derived from 7-hydroxycoumarin are described. These hemicyanine-coumarin hybrids display promising spectroscopic features such as large apparent Stokes shift (ranging from 60 to 140 nm) and fluorescence emission maxima between 620 and 720 nm in physiological conditions. Their utility was then illustrated by the preparation of an original fluorogenic probe of penicillin G acylase (PGA) whose fluorescence is unveiled through an enzyme-initiated domino reaction.
Richard
Jean-Alexandre
JA
Equipe de Chimie Bio-Organique, Université de Rouen, Place Emile Blondel, 76821 Mont-Saint-Aignan, France.
Massonneau
Marc
M
Renard
Pierre-Yves
PY
Romieu
Anthony
A