Immobilization of hemoglobin on the gold colloid modified pretreated glassy carbon electrode for preparing a novel hydrogen peroxide biosensor.
A novel hydrogen peroxide (H2O2) biosensor was developed by immobilizing hemoglobin on the gold colloid modified electrochemical pretreated glassy carbon electrode (PGCE) via the bridging of an ethylenediamine monolayer. This biosensor was characterized by UV-vis reflection spectroscopy (UV-vis), electrochemical impendence spectroscopy (EIS) and cyclic voltammetry (CV). The immobilized Hb exhibited excellent electrocatalytic activity for hydrogen peroxide. The Michaelis-Menten constant (K(m)) was 3.6 mM. The currents were proportional to the H2O2 concentration from 2.6 x 10(-7) to 7.0 x 10(-3) M, and the detection limit was as low as 1.0 x 10(-7) M (S/N = 3).
Liu
Yang
Y
Institute of Analytical Chemistry for Life Science, School of Public Health, Nantong University, Nantong 226007, China.
Jiang
Qi-Yu
QY
Lu
Shuang-Yun
SY
Zhang
Ye
Y
Gu
Hai-Ying
HY