NCAM as a cystogenesis marker gene of PKD2 overexpression.
ADPKD (Autosomal Dominant Polycystic Kidney Disease) is characterized by the progressive expansion of multiple cystic lesions in the kidneys. ADPKD is caused by mutations in Ed-pl. consider PKD1 and PKD2. Recently a relation between c-myc and the pathogenesis of ADPKD was reported. In addition, c-Myc is a downstream effector of PKD1. To identify the gene regulated by PKD2 and c-Myc, we performed gene expression profiling in PKD2 and c-Myc overexpressing cells using a human 8K cDNA microarray. NCAM (neuronal cell adhesion molecule) levels were significantly reduced in PKD2 overexpressing systems in vitro and in vivo. These results suggest that NCAM is an important molecule in the cystogenesis induced by PKD2 overexpression.
Yoo
Kyung Hyun
KH
Department of Biological Science, Sookmyung Women's University, Seoul 140-742, Korea.
Lee
Tae Young
TY
Yang
Moon Hee
MH
Park
Eun Young
EY
Yook
Yeon Joo
YJ
Lee
Hyo Soo
HS
Park
Jong Hoon
JH