[Personal identification of an unknown individual based on determination of his DNA profile from exhumed remains].
The aim of the present investigation was personal identification of an unknown man whose remains were exhumed four years after burial. The femur of the deceased was secured for the genetic analysis. The comparative material included buccal swabs collected from the putative relatives of the deceased, i.e. the wife, son and brother. Genomic DNA was extracted from the bone using two methods: traditional isolation with phenol/chloroform and as a alternative technique, a simple and rapid method described by T. Kalmár et al. The results were then compared. The specimens underwent DNA amplification using the AmpFISTRSEfiler PCR Amplification Kit. The authors obtained a full STR profile of the unknown man from each isolate, yet the DNA extraction method proposed by T. Kalmár et al. allowed for simpler and faster isolation of genetic material. The statistical analysis of the obtained results confirmed the paternity of the deceased and established his son as his rightful child (P = 99.999999%), also confirming the consanguinity between the investigated individual and his putative brother (P = 99.9999%).
Kapińska
Ewa
E
Katedra i Zakład Medycyny Sadowej Akademii Medycznej w Gdańsku. kapiniaczek@wp.pl
Szczerkowska
Zofia
Z