Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays.
A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.
Yang
S Y
SY
Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan.
Chieh
J J
JJ
Wang
W C
WC
Yu
C Y
CY
Lan
C B
CB
Chen
J H
JH
Horng
H E
HE
Hong
Chin-Yih
CY
Yang
H C
HC
Huang
Wilber
W