title: Peroxidase-like catalytic activity of aqueous- and immobilized-Mn(3+)-octabromo-porphyrins on ion-exchange resin supplied as mimetic of horseradish peroxidase.
abstract: In order to explore the capability of metal porphyrins as an alternative of horseradish peroxidase (HRP), HRP-like activity of three manganese-porphyrins (Mn-Ps) and three Mn-octabromo-porphyrins (Mn-OBPs) was examined in both aqueous and immobilized states. It was found that Mn(3+)-octabromotetrakis(1-methyl-pyridinium-4yl)porphine (Mn-OBTMPyP) has an activity of at least 90% of HRP in an aqueous solution. Mn-OBTMPyP exhibited a catalytic activity even in the presence of hydrogen peroxide without suicide reaction. In addition, Mn-OBTMPyP was revealed to function as an alternative to HRP in the quantitative determination of serum uric acid. These results are of great interest because they indicate that metal-octabromo-porphyrins possibly include promising candidates of artificial enzyme capable of substituting for HRP.
authors.affiliation: Department of Pharmaceutical Sciences, Graduate School of Medicine and Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan. kitamura@pharm.okayama-u.ac.jp
authors.forename: Youji
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Katsuya
authors.initials: K
authors.lastname: Mori
authors.forename: Makiko
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Nozaki
authors.forename: Madoka
authors.initials: M
authors.lastname: Saito
authors.forename: Ikuko
authors.initials: I
authors.lastname: Tsukamoto
authors.forename: Masaki
authors.initials: M
authors.lastname: Mifune
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Saito