title: Effects of TFAR19 gene on the growth and biorheological properties of mouse erythroleukemia cell line MEL.
abstract: Using the method of gene transfection with liposome, we obtained the mouse erythroleukemia cell line MEL-TF19, which stably carries TFAR19, a novel apoptosis-related gene. The expression of TFAR19 was detected by Western blot. Growth curve and flow cytometry analysis showed that after being transfected with TFAR19 gene, the growth of MEL-TF19 is suppressed and its apoptosis is accelerated because of the serum deprivation. Our biorheological study indicated that in the apoptotic process, compared with MEL cells, MEL-TF19 cells exhibit larger osmotic fragility, lower cell surface charge density, increased elastic modulus K (1) which is inversely proportional to cells' maximal deformation ability, obviously diminished surface viscosity mu, with elastic modulus K (2)having no distinct changes. The above results provided some bases for recognizing the function of TFAR19 completely from the viewpoint of biorheology.
authors.affiliation: Hemarheology Center, School of Basic Medical Science, Health Science Center, Peking University, 100083, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gu
authors.forename: Weijuan
authors.initials: W
authors.lastname: Yao
authors.forename: Zongyi
authors.initials: Z
authors.lastname: Yan
authors.forename: Lide
authors.initials: L
authors.lastname: Xie
authors.forename: Dagong
authors.initials: D
authors.lastname: Sun
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zeng
authors.forename: Zongyao
authors.initials: Z
authors.lastname: Wen