title: Evidence that the (6-4) photolyase mechanism can proceed through an oxetane intermediate.
abstract: Using the analogue of TpT methylated at the 3'-end N3 position (Tpm3T), we demonstrate that when the oxetane/(6-4) pathway is precluded, water addition occurs at the 3'-end C6 position of the oxetane intermediate to provide its opening. Photoreversal of this (6-4) photoproduct C6 hydrate brings the first experimental evidence that the (6-4) photolyase repair can proceed through an oxetane intermediate.
authors.affiliation: Université de Reims Champagne Ardenne, Institut de Chimie Moléculaire de Reims, CNRS UMR 6229, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims Cedex, France.
authors.forename: Saâdia
authors.initials: S
authors.lastname: Asgatay
authors.forename: Christian
authors.initials: C
authors.lastname: Petermann
authors.forename: Dominique
authors.initials: D
authors.lastname: Harakat
authors.forename: Dominique
authors.initials: D
authors.lastname: Guillaume
authors.forename: John-Stephen
authors.initials: JS
authors.lastname: Taylor
authors.forename: Pascale
authors.initials: P
authors.lastname: Clivio