title: Adoptive T cell transfer augments IL-2 mediated tumour regression in a HNSCC xenograft nude mouse model.
abstract: The potential of cytokine gene therapy using naked plasmid DNA encoding human IL-2 cDNA in a xenograft nude mouse model was investigated. To overcome the T cell deficiency in nude mice, Adoptive T Cell Transfer [ATCT] into tumour bearing nude mice was performed. The migration of T cells was tracked in vivo using CFSE labeling. IL-2 in circulation was observed till day 15 with the levels reaching a peak by day 3. Significant activation of transferred T cells (CD69+, CD25+) as well as significant increase in levels of NK cells (CD49b+) was seen in animals receiving IL-2. Tumour regression was observed in the IL-2+ ATCT group.
authors.affiliation: Advanced Center for Treatment Research and Education in Cancer [ACTREC], Tata Memorial Centre, Kharghar, Navi Mumbai 410210, India.
authors.forename: Aditya
authors.initials: A
authors.lastname: Ambade
authors.forename: Rita
authors.initials: R
authors.lastname: Mulherkar