title: Experimental reproduction of the runting-stunting syndrome of broiler chickens.
abstract: A model for the reproduction of the runting-stunting syndrome (RSS) of broiler chickens is described. In this model, groups of at least 90 day-old broiler chickens were inoculated (per os) with various tissue homogenates or virus preparations. During the first week post-inoculation, birds were examined for the development of histopathological changes in their intestines. At day 14 post-inoculation, the remaining birds were weighed and tested for elevations in plasma amylase activity and examined for the development of pancreatic atrophy. Bacteria-free intestinal and pancreatic homogenates from chickens of different ages, taken from flocks which developed RSS, regularly induced a lower mean live-weight in treated birds. Of these, only intestinal homogenates prepared from 5-day-old birds induced intestinal lesions, lowered mean live-weight and increased the incidence of both elevated plasma amylase activity and pancreatic atrophy. These changes were more marked in birds exposed to short periods of sub-optimal temperatures during the first week post-inoculation. An ultracentrifuged pellet prepared from this intestinal homogenate, was also found to induce an increased incidence of pancreatic atrophy in treated birds. These studies suggest that the causative agent(s) of RSS is an as yet unidentified virus, and that the effects of this infection are greater in birds subjected to stress, such as sub-optimal temperature exposure, within the first week of hatch.
authors.affiliation: Department of Agriculture and Rural Affairs, Veterinary Research Institute, Parkville, Victoria, Australia.
authors.forename: I J
authors.initials: IJ
authors.lastname: Smart
authors.forename: D A
authors.initials: DA
authors.lastname: Barr
authors.forename: R L
authors.initials: RL
authors.lastname: Reece
authors.forename: W M
authors.initials: WM
authors.lastname: Forsyth
authors.forename: I
authors.initials: I
authors.lastname: Ewing