title: Structural and functional homology among chicken, duck, goose, turkey and pigeon interleukin-8 proteins.
abstract: Interleukin (IL)-8-encoding regions of five avian species were cloned, sequenced and characterized. Each IL-8-encoding region is 312 nucleotides long and encodes IL-8 which is 103 amino acids. Pairwise sequence analysis showed that sequence identities of IL-8-encoding regions ranged from 87% to 100%. The IL-8 protein identities varied from 84% to 100%. Phylogenetic analysis indicated that IL-8-encoding regions and encoded proteins of chicken, duck, goose and turkey clustered together and evolved into a distinct phylogenetic lineage from that of pigeon which evolved into a second lineage. The results from binding reactivities of antiserum against each recombinant IL-8 (rIL-8) protein to homologous or heterologous rIL-8 proteins, chemotactic activities of each rIL-8 protein or reduction levels of the chemotactic activity of rIL-8 protein which was pretreated with homologous or heterlogous antiserum have suggested that all five IL-8 proteins were functionally active, and shared structural and functional identity with each other.
authors.affiliation: Graduate Institute of Veterinary Microbiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Yung Fu
authors.initials: YF
authors.lastname: Wu
authors.forename: Jui Huang
authors.initials: JH
authors.lastname: Shien
authors.forename: Hsien Hseng
authors.initials: HH
authors.lastname: Yin
authors.forename: Shiou Her
authors.initials: SH
authors.lastname: Chiow
authors.forename: Long Huw
authors.initials: LH
authors.lastname: Lee