title: Examination of isomer specific bioaccumulation parameters and potential in vivo hepatic metabolites of syn- and anti-Dechlorane Plus isomers in juvenile rainbow trout (Oncorhynchus mykiss).
abstract: Juvenile rainbow trout (Oncorhynchus mykiss) were exposed in the laboratory to elevated doses of syn- and anti-isomers of Dechlorane Plus (DP) via their diet for 49 days (uptake phase), followed by 112 days of untreated food (depuration phase) to examine bioaccumulation parameters and possible metabolic products. Three groups of 60 fish were used in the study. Two groups were exposed separately to food fortified with known concentrations of syn- (0.79 +/- 0.03 microg/g, lipid weight) and anti-DP (1.17 +/- 0.12 microg/g, lipid weight) while a third control group was fed unfortified food. Neither isomer reached steady-state after 49 days of exposure. Only the syn-isomer accumulated linearly in the fish (whole-body minus liver) during the dosing phase with a calculated uptake rate constant of 0.045 +/- 0.005 (arithmetic mean +/- 1 x standard error) nmoles per day. A similar uptake rate was also observed for this isomer in the liver. The elimination of both isomers from the whole fish (minus liver) obeyed first order depuration kinetics (syn-: r2 = 0.6427, p < 0.001, anti-: r2 = 0.5350, p < 0.005) with calculated half-lives (t1/2) of 53.3 +/- 13.1 (syn-) and 30.4 +/- 5.7 (anti-) days. Elimination of the isomers from the liver was difficult to interpret because of suspected enterohepatic circulation and redistribution of the isomers in the liver during clearance from other tissues. The biomagnification factor (BMF, determined in whole fish minus liver) of the syn-isomer (5.2) was greater than the anti-isomer (1.9) suggesting that the former isomer is more bioavailable. A suite of metabolites were screened for in the liver including dechlorinated, hydroxylated, methoxylated and methyl sulfone degradates. Even with the purposely high dose used in the uptake phase, none of these degradates could be detected in the extracts. This suggests that if metabolites of DP are detected in fish from aquatic food webs their presence is likely not from in vivo biotransformation of the parent compound.
authors.affiliation: Department of Fisheries and Oceans, Arctic Aquatic Research Division, Winnipeg, MB R3T 2N6, Canada. gregg.tomy@dfo-mpo.gc.ca
authors.forename: Gregg T
authors.initials: GT
authors.lastname: Tomy
authors.forename: Caden R
authors.initials: CR
authors.lastname: Thomas
authors.forename: Thane M
authors.initials: TM
authors.lastname: Zidane
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Murison
authors.forename: Kerri
authors.initials: K
authors.lastname: Pleskach
authors.forename: Jonathon
authors.initials: J
authors.lastname: Hare
authors.forename: Gilles
authors.initials: G
authors.lastname: Arsenault
authors.forename: Chris H
authors.initials: CH
authors.lastname: Marvin
authors.forename: Ed
authors.initials: E
authors.lastname: Sverko