title: Hyperoside prevents oxidative damage induced by hydrogen peroxide in lung fibroblast cells via an antioxidant effect.
abstract: We elucidated the cytoprotective effects of hyperoside (quercetin-3-O-galactoside) against hydrogen peroxide (H2O2)-induced cell damage. We found that hyperoside scavenged the intracellular reactive oxygen species (ROS) detected by fluorescence spectrometry, flow cytometry, and confocal microscopy. In addition, we found that hyperoside scavenged the hydroxyl radicals generated by the Fenton reaction (FeSO4)+H2O2) in a cell-free system, which was detected by electron spin resonance (ESR) spectrometry. Hyperoside was found to inhibit H2O2-induced apoptosis in Chinese hamster lung fibroblast (V79-4) cells, as shown by decreased apoptotic nuclear fragmentation, decreased sub-G(1) cell population, and decreased DNA fragmentation. In addition, hyperoside pretreatment inhibited the H2O2-induced activation of caspase-3 measured in terms of levels of cleaved caspase-3. Hyperoside prevented H2O2-induced lipid peroxidation as well as protein carbonyl. In addition, hyperoside prevented the H2O2-induced cellular DNA damage, which was established by comet tail, and phospho histone H2A.X expression. Furthermore, hyperoside increased the catalase and glutathione peroxidase activities. Conversely, the catalase inhibitor abolished the cytoprotective effect of hyperoside from H2O2-induced cell damage. In conclusion, hyperoside was shown to possess cytoprotective properties against oxidative stress by scavenging intracellular ROS and enhancing antioxidant enzyme activity.
authors.affiliation: Department of Biochemistry, School of Medicine, Institute of Medical Science, Cheju National University, Jeju-si 690-756, Korea.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Dong Ok
authors.initials: DO
authors.lastname: Ko
authors.forename: Zhi Hong
authors.initials: ZH
authors.lastname: Wang
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: You
authors.forename: Hee Sun
authors.initials: HS
authors.lastname: Kim
authors.forename: Ju Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Sam Sik
authors.initials: SS
authors.lastname: Kang
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun