Functional implications of the conserved action of regulators of ribonuclease activity.
RNase E (Rne) plays a major role in the decay and processing of numerous RNAs in E. coli, and protein inhibitors of RNase E, RraA and RraB, have recently been discovered. Here, we report that coexpression of RraA or RraB reduces the ribonucleolytic activity in rne-deleted E. coli cells overproducing RNase ES, a Streptomyces coelicolor functional ortholog of RNase E, and consequently rescues these cells from growth arrest. These findings suggest that the regulators of ribonuclease activity have a conserved intrinsic property that effectively acts on an RNase E-like enzyme found in a distantly related bacterial species.
Yeom
Ji-Hyun
JH
Department of Life Science, Chung-Ang University, Seoul 156-756, Korea.
Shin
Eunkyoung
E
Go
Hayoung
H
Sim
Se-Hoon
SH
Seong
Maeng-Je
MJ
Lee
Kangseok
K