title: Evaluation of EDTA hematology tubes for collection of blood samples for tests of secondary hemostasis in dogs.
abstract: OBJECTIVE
  To evaluate the use of EDTA tubes for collection of blood samples for assays of secondary hemostasis in dogs.
  
  
  METHODS
  108 dogs of various ages, breeds, and sexes (19 healthy and 89 with abnormalities of secondary hemostasis).
  
  
  METHODS
  Blood samples were collected via cephalic venipuncture and transferred to sodium citrate tubes and EDTA tubes. Plasma was harvested from each type of tube for assays of concentrations of fibrinogen and D-dimer as well as prothrombin time, activated partial thromboplastin time, and antithrombin activity. Intra-assay and interassay precision and correlation coefficients for all hemostatic tests were calculated for each type of plasma sample. The effect of storage conditions on assay results for the 2 types of plasma samples was also evaluated.
  
  
  RESULTS
  Results of hemostatic tests were highly correlated between citrated and EDTA-treated plasma samples. Intra-assay imprecision for all hemostatic tests with the exception of D-dimer concentration was < 10% for both citrated and EDTA-treated plasma samples; interassay imprecision was higher for EDTA-treated versus citrated plasma samples. Storage of plasma samples for 1 hour did not result in significantly different assay results for either type of plasma sample, but storage for 2 hours significantly affected values for EDTA-treated plasma samples.
  
  
  CONCLUSIONS
  Although evaluation of the sensitivity and specificity of hemostatic tests that use EDTA-treated plasma samples is required, EDTA may be a suitable alternative to sodium citrate as an anticoagulant for use in hemostatic testing in conditions in which tests could be performed within 1 hour after sample collection.
authors.affiliation: Animal Medicine and Surgery Department, Faculty of Veterinary Medicine, Campus de Espinardo, University of Murcia, Murcia 30100, Spain.
authors.forename: Jose J
authors.initials: JJ
authors.lastname: Cerón
authors.forename: Erika
authors.initials: E
authors.lastname: Carli
authors.forename: Silvia
authors.initials: S
authors.lastname: Tasca
authors.forename: Silvia
authors.initials: S
authors.lastname: Martinez-Subiela
authors.forename: Marco
authors.initials: M
authors.lastname: Caldin