title: [Expression and significance of B7-H1 and its receptor PD-1 in human gastric carcinoma].
abstract: OBJECTIVE
  The B7-H1/PD-1 co-signaling pathway has recently been found to play a pivotal role in the immune evasion of tumor cells from host immune system. The aim of this study was to examine the B7-H1 and PD-1 expression and TILs status in gastric cancer and to elucidate the clinical relevance of B7-H1 and PD-1 to the pathogenesis of gastric carcinoma.
  
  
  METHODS
  Immunohistochemistry and ANAE histochemical staining were used to investigate the in situ expression of B7-H1 and PD-1 and TILs status in the gastric tissues. RT-PCR was used to explore B7-H1 and PD-1 expression at the transcriptional level. The B7-H1 expression at protein level was detected by Western blot.
  
  
  RESULTS
  Expression of B7-H1 and PD-1 was found to be increased in gastric carcinoma, but absent in normal gastric tissue. B7-H1 expression in gastric carcinoma was inversely correlated with TILs infiltration. B7-H1 but not PD-1 expression in tumor tissue was significantly correlated with some clinicopathhological variables including depth of invasion, lymph node metastasis and distant metastasis.
  
  
  CONCLUSIONS
  B7-H1 and PD-1 expressions are increased in gastric carcinoma. This signaling pathway may inhibit antitumor immune responses in gastric carcinoma. B7-H1 expression plays a critical role in the pathogenesis of human gastric carcinoma,and might be a promising prognostic marker and therapeutic target in the treatment of this disease.
authors.affiliation: Department of Histology and Embryology, College of Medicine, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Shu-Man
authors.initials: SM
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Meng
authors.forename: Qin-Xian
authors.initials: QX
authors.lastname: Zhang
authors.forename: Sheng-Dian
authors.initials: SD
authors.lastname: Wang
authors.forename: Zhan-Ju
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Xie-Fu
authors.initials: XF
authors.lastname: Zhang