title: Structure, expression pattern and chromosomal localization of the rice Osgrp-2 gene.
abstract: Glycine-rich proteins (GRPs) belong to a kind of important structural proteins of plant cell walls. The expression of GRP genes is regulated spatially and developmentally as well as by various environmental stresses, thus providing a good model for the study of plant gene expression. We obtained the genomic sequence of a new GRP gene (Osgrp- 2) from a rice genomic library. The transcription start site of Osgrp- 2 was determined by 5'-rapid amplification of cDNA ends (RACE) and a 2.4-kb promoter sequence was thus delimited. The spatial and developmental expression pattern as well as the wound-inducible character of Osgrp- 2 in rice plants was analyzed in detail. Furthermore, the gene was mapped onto rice chromosome 10 by analysis of restriction fragment length polymorphism (RFLP).
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, China.
authors.forename: Zongzhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Jianlong
authors.initials: J
authors.lastname: Wang
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.forename: Xun
authors.initials: X
authors.lastname: Huang
authors.forename: Weihui
authors.initials: W
authors.lastname: Xu
authors.forename: Lihuang
authors.initials: L
authors.lastname: Zhu
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.forename: Rongxiang
authors.initials: R
authors.lastname: Fang