title: Increased sperm ubiquitination correlates with abnormal chromatin integrity.
abstract: Ubiquitin, a 8.5 kDa peptide that marks other proteins for proteasomal degradation, tags defective spermatozoa during epididymal passage and is proposed as a biomarker for sperm quality. The present study was designed to evaluate the relationships between sperm ubiquitination, sperm chromatin integrity and semen parameters. Semen samples from 63 couples were collected and analysed according to World Health Organization criteria. Each sample was evaluated for sperm ubiquitination by the direct immunofluorescence method, using anti-ubiquitin antibodies. Chromatin integrity of the same samples was analysed using acridine orange (AO) and toluidine blue (TB) tests. A positive correlation was found between ubiquitinated spermatozoa and the percentage of spermatozoa with abnormal chromatin (AO: r = 0.58, P < 0.001 and TB: r = 0.48, P < 0.001). Negative correlations were obtained between sperm ubiquitination and: sperm count (r = -0.2, P = 0.048), sperm morphology (r = -0.36, P = 0.003), rapidly progressive motility (r = -0.25, P = 0.044) and slow progressive motility (r = -0.28, P = 0.022). Sperm ubiquitination was positively correlated with the percentage of immotile spermatozoa. These results show that among semen parameters, chromatin abnormality is more closely associated with sperm ubiquitination and further validate sperm ubiquitination as a suitable marker for sperm quality.
authors.affiliation: Reproductive Biotechnology Research Centre, Avicenna Research Institute, Evin, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Hodjat
authors.forename: M A
authors.initials: MA
authors.lastname: Akhondi
authors.forename: S
authors.initials: S
authors.lastname: Al-Hasani
authors.forename: M
authors.initials: M
authors.lastname: Mobaraki
authors.forename: M R
authors.initials: MR
authors.lastname: Sadeghi