title: Microdissection of additional chromosome in common wheat-Th. intermedium TAI-27 and screening of its special probe.
abstract: It was identified that there were 4 St chromosomes derived from Th. intermedium in common wheat-Th. intermedium alien additional line TAI-27 by in situ hybridization. Two St chromosomes added to wheat chromosome in TAI-27 as well as two of them replaced two of 42 in wheat chromosomes. This indicates that TAI-27 is not only an alien additional line, but also a replacing line. The additional chromosomes in TAI-27 were microdissected and a microcloning library was constructed. A special probe of Th. intermedium was obtained from a microcloning library. The sequence analysis indicated that there were no homology with Genebank data. This makes it possible to screen genes with the disease-resistance, adversity-tolerance and fine character from Th.intermedium.
authors.affiliation: Institute of Plant Science and Technology, Chinese Agricultural University, Beijing, China.
authors.forename: C
authors.initials: C
authors.lastname: Tian
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: J
authors.initials: J
authors.lastname: Deng
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Liu