title: The osteogenic differentiation of rat muscle-derived stem cells in vivo within in situ-forming chitosan scaffolds.
abstract: We herein examined the bone formation from rat muscle-derived stem cells (rMDSCs) using an injectable in situ-forming chitosan gel in vivo. The rMDSCs were easily isolated from rat muscle tissue. The osteogenic factors caused differentiation of rMDSCs toward the osteogenic lineage. The rMDSCs survived well on the scaffold created by the in vitro and in vivo in situ-forming chitosan gel, indicating that in situ gel-forming chitosan was a suitable substrate for the attachment and proliferation of rMDSCs. Bone formation was observed only in chitosan gel containing both rMDSCs and osteogenic factors. Subcutaneous implantation of the in situ-forming chitosan gel demonstrated that rMDSCs-containing chitosan gel induced much lower host tissue responses than did the chitosan gel alone, probably due to the immunosuppression of the transplanted rMDSCs.
authors.affiliation: Fusion Biotechnology Research Center, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseung, Daejon 305-606, Republic of Korea.
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Kim
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Hyun Hee
authors.initials: HH
authors.lastname: Ahn
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
authors.forename: Bong
authors.initials: B
authors.lastname: Lee
authors.forename: Hai Bang
authors.initials: HB
authors.lastname: Lee
authors.forename: Moon Suk
authors.initials: MS
authors.lastname: Kim