Microcarrier-based MDCK cell culture system for the production of influenza H5N1 vaccines.
Current egg-based influenza vaccine production technology, which is labor intensive and slow, would not be able to meet demand during an influenza pandemic. Thus, interest in the emerging technology of using mammalian cells for vaccine production has been great. In this study, Madin-Darby canine kidney (MDCK) cells using microcarrier culture systems were established to produce inactivated whole-virus H5N1 vaccine. The current clade-1 influenza H5N1 vaccine virus (NIBRG-14) was provided by the UK National Institute for Biological Standards and Control. Various process parameters were first optimized in 100-mL scale spinner flasks then scaled up to a 1-L scale bioreactor system. In the 1-L scale bioreactor system, peak virus titer could reach 10(8-9)TCID50/mL using serum-containing medium. After purification and inactivation, hemagglutinin (HA) protein content reached 31.56-43.96 microg/mL in two different runs. In mice immunogenicity studies, two doses of the purified vaccine antigen adjuvanted with aluminum phosphate induced good immune responses in 0.2 and 1.0 microg HA dosages (geometric mean titers of hemagglutination-inhibition antibody: 113 and 242, respectively). This study demonstrates the feasibility of the development of MDCK cell-based inactivated influenza H5 vaccines in microcarrier culture systems and could be valuable to many countries that are planning to establish manufacturing capacity for influenza vaccines.
Hu
Alan Yung-Chih
AY
Vaccine R&D center, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
Weng
Tsai-Chuan
TC
Tseng
Yu-Fen
YF
Chen
Yu-Shuan
YS
Wu
Chia-Hann
CH
Hsiao
Sigrid
S
Chou
Ai-Hsiang
AH
Chao
Hsin-Ju
HJ
Gu
Anna
A
Wu
Suh-Chin
SC
Chong
Pele
P
Lee
Min-Shi
MS