title: Gene conversion and end-joining-repair double-strand breaks in the Caenorhabditis elegans germline.
abstract: Excision of a Mos1 transposon in the germline of Caenorhabditis elegans generates a double-strand break in the chromosome. We demonstrate that breaks are most prominently repaired by gene conversion from the homolog, but also rarely by nonhomologous end-joining. In some cases, gene conversion events are resolved by crossing over. Surprisingly, expression of the transposase using an intestine-specific promoter can induce repair, raising the possibility that activation of transposase expression in somatic cells can lead to transposition of Mos1 in the germline.
authors.affiliation: Ecole Normale Supérieure, Biologie Cellulaire de la Synapse, Paris, France.
authors.forename: Valérie J
authors.initials: VJ
authors.lastname: Robert
authors.forename: M Wayne
authors.initials: MW
authors.lastname: Davis
authors.forename: Erik M
authors.initials: EM
authors.lastname: Jorgensen
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Bessereau