title: Development of an ELA-DRA gene typing method based on pyrosequencing technology.
abstract: The polymorphism of equine lymphocyte antigen (ELA) class II DRA gene had been detected by polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) and reference strand-mediated conformation analysis. These methodologies allowed to identify 11 ELA-DRA exon 2 sequences, three of which are widely distributed among domestic horse breeds. Herein, we describe the development of a pyrosequencing-based method applicable to ELA-DRA typing, by screening samples from eight different horse breeds previously typed by PCR-SSCP. This sequence-based method would be useful in high-throughput genotyping of major histocompatibility complex genes in horses and other animal species, making this system interesting as a rapid screening method for animal genotyping of immune-related genes.
authors.affiliation: Centro de Investigaciones en Genética Básica y Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina. sdiaz@fcv.unlp.edu.ar
authors.forename: S
authors.initials: S
authors.lastname: Díaz
authors.forename: M G
authors.initials: MG
authors.lastname: Echeverría
authors.forename: V
authors.initials: V
authors.lastname: It
authors.forename: D M
authors.initials: DM
authors.lastname: Posik
authors.forename: A
authors.initials: A
authors.lastname: Rogberg-Muñoz
authors.forename: N L
authors.initials: NL
authors.lastname: Pena
authors.forename: P
authors.initials: P
authors.lastname: Peral-García
authors.forename: J L
authors.initials: JL
authors.lastname: Vega-Pla
authors.forename: G
authors.initials: G
authors.lastname: Giovambattista