title: Phenotype of blasts in chronic myeloid leukemia in blastic phase-Analysis of bone marrow trephine biopsies and correlation with cytogenetics.
abstract: We identified different phenotypic subsets among 62 cases of chronic myeloid leukemia (CML) in blast crisis (BC) (26% B-lymphoblastic, and 74% various myeloblastic subsets) on bone marrow trephines and correlated the blast-phenotype with cytogenetics. Five of myeloid-BC had an associated 3q26 abnormality and two of these showed a megakaryoblastic-phenotype. While myeloid-BC was associated with additional copies of Philadelphia (Ph) (29%) (p=0.08), numerical abnormalities (51%) (p=0.007), trisomy-8 (29%) (p=0.08) and 17p-loss (22%), none of lymphoid-BC showed these abnormalities. Among myeloid-BC, CD34-negative cases were more often associated with trisomy-8, 17p-loss and numerical abnormalities, and the CD117-negative subset with additional copies of Ph (p<0.05).
authors.affiliation: Departments of Histopathology and Haematology, Hammersmith Hospital & Imperial College, London, UK.
authors.forename: Alistair G
authors.initials: AG
authors.lastname: Reid
authors.forename: Valeria A
authors.initials: VA
authors.lastname: De Melo
authors.forename: Kay
authors.initials: K
authors.lastname: Elderfield
authors.forename: Ian
authors.initials: I
authors.lastname: Clark
authors.forename: David
authors.initials: D
authors.lastname: Marin
authors.forename: Jane
authors.initials: J
authors.lastname: Apperley
authors.forename: Kikkeri N
authors.initials: KN
authors.lastname: Naresh