title: Estimating weak ratiometric signals in imaging data. II. Meta-analysis with multiple, dual-channel datasets.
abstract: Ratiometric fluorescent indicators are used for making quantitative measurements of a variety of physiological variables. Their utility is often limited by noise. This is the second in a series of papers describing statistical methods for denoising ratiometric data with the aim of obtaining improved quantitative estimates of variables of interest. Here, we outline a statistical optimization method that is designed for the analysis of ratiometric imaging data in which multiple measurements have been taken of systems responding to the same stimulation protocol. This method takes advantage of correlated information across multiple datasets for objectively detecting and estimating ratiometric signals. We demonstrate our method by showing results of its application on multiple, ratiometric calcium imaging experiments.
authors.affiliation: Department of Mathematics and Faculty of Engineering, University of Georgia, AThens, GA 30602, USA. ats@math.uga.edu
authors.forename: Andrew
authors.initials: A
authors.lastname: Sornborger
authors.forename: Josef
authors.initials: J
authors.lastname: Broder
authors.forename: Anirban
authors.initials: A
authors.lastname: Majumder
authors.forename: Ganesh
authors.initials: G
authors.lastname: Srinivasamoorthy
authors.forename: Erika
authors.initials: E
authors.lastname: Porter
authors.forename: Sean S
authors.initials: SS
authors.lastname: Reagin
authors.forename: Charles
authors.initials: C
authors.lastname: Keith
authors.forename: James D
authors.initials: JD
authors.lastname: Lauderdale