title: Improved approach for analyzing bromophenols in seafood using stable isotope dilution analysis in combination with SPME.
abstract: An analytical method for the measurement of five naturally occurring bromophenols of sensory relevance in seafood (barramundi and prawns) is presented. The method combines simultaneous distillation-extraction followed by alkaline back extraction of a hexane extract and subsequent acetylation of the bromophenols. Analysis of the bromophenol acetates was accomplished by headspace solid phase microextraction and gas chromatography-mass spectrometry using selected ion monitoring. The addition of (13)C 6 bromophenol stable isotope internal standards for each of the five congeners studied permitted the accurate quantitation of 2-bromophenol, 4-bromophenol, 2,6-dibromophenol, 2,4-dibromophenol, and 2,4,6-tribromophenol down to a limit of quantification of 0.05 ng/g of fish flesh. The method indicated acceptable precision and repeatability and excellent linearity over the typical concentration range of these compounds in seafood (0.5-50 ng/g). The analytical method was applied to determine the concentration of bromophenols in a range of farmed and wild barramundi and prawns and was also used to monitor bromophenol uptake in a pilot feeding trial.
authors.affiliation: Department of Primary Industries and Fisheries, Emerging Technologies, 19 Hercules Street, Hamilton, Queensland 4007, Australia. steve.fuller@dpi.qld.gov.au
authors.forename: Steve C
authors.initials: SC
authors.lastname: Fuller
authors.forename: Damian C
authors.initials: DC
authors.lastname: Frank
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Fitzhenry
authors.forename: Heather E
authors.initials: HE
authors.lastname: Smyth
authors.forename: Sue E
authors.initials: SE
authors.lastname: Poole