title: Large inverted repeats in the vicinity of a single double-strand break strongly affect repair in yeast diploids lacking Rad51.
abstract: DNA double-strand breaks (DSBs) are critical lesions that can lead to cell death or chromosomal rearrangements. Rad51 is necessary for most mitotic and meiotic DSB repair events, although a number of RAD51-independent pathways exist. Previously, we described DSB repair in rad51Delta yeast diploids that was stimulated by a DNA region termed "facilitator of break-induced replication" (FBI) located approximately 30kb from the site of an HO-induced DSB. Here, we demonstrate that FBI is a large inverted DNA repeat that channels the repair of DSBs into the single-strand annealing-gross chromosomal rearrangements (SSA-GCR) pathway. Further, analysis of DSB repair in rad54Delta cells allowed us to propose that the SSA-GCR repair pathway is suppressed in the presence of Rad51p. Therefore, an additional role of Rad51 might be to protect eukaryotic genomes from instabilities by preventing chromosomal rearrangements.
authors.affiliation: Department of Biology, School of Science, IUPUI, Indianapolis, IN 46202-5132, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Downing
authors.forename: Rachel
authors.initials: R
authors.lastname: Morgan
authors.forename: Kelly
authors.initials: K
authors.lastname: VanHulle
authors.forename: Angela
authors.initials: A
authors.lastname: Deem
authors.forename: Anna
authors.initials: A
authors.lastname: Malkova