[Relationship between alterations of circulating cell-derived microparticles and glucocorticosteroid application].
OBJECTIVE
To explore the relationship between the alterations of circulating cell-derived microparticles (MPs) and large-dose glucocorticosteroid application.


METHODS
Peripheral blood samples were collected from 33 patients with history of large-dose glucocorticosteroid application (glucocorticosteroid group) and 24 age-, sex-, and race-matched healthy controls (healthy control group). Platelet-poor plasma was obtained by centrifugation. Plasma microparticles were labeled with monoclonal antibodies of PE-conjugated mouse anti-human CD31, CD54, and FITC-conjugated mouse anti-human CD42b, CD45, CD51/61, CD105, and PE-Cy5-conjugated mouse anti-human CD62E. Cell-derived microparticles were measured by three-color flow cytometry.


RESULTS
The mean ranks of CD31+ MPs, CD45+ MPs, CD51/61 MPs, CD31+ CD42b+ MPs, and CD31+ CD45+ MPs of the glucocorticosteroid group were: 24.1, 25.5, 25.6, 21.6, and 24.8 respectively, all significantly lower than those of the control group (35.7, 33.8, 33.7, 39.3, and 34.8 respectively, P = 0.009, 0.019, 0.045, 0.000, and 0.009).


CONCLUSIONS
High-dose glucocorticosteroid application remarkably reduces plasma MPs that may be responsible for microcirculation disturbance.
Gao
Zeng-xin
ZX
Department of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, China.
Weng
Xi-sheng
XS
Qiu
Gui-xing
GX
Wu
Zhi-hong
ZH
Cui
Wei
W
Shao
Bai
B
Mei
Su-ping
SP
Xiao
Jun
J
Yang
Xin-yu
XY