title: Preliminary X-ray diffraction analysis of YqjH from Escherichia coli: a putative cytoplasmic ferri-siderophore reductase.
abstract: YqjH is a cytoplasmic FAD-containing protein from Escherichia coli; based on homology to ViuB of Vibrio cholerae, it potentially acts as a ferri-siderophore reductase. This work describes its overexpression, purification, crystallization and structure solution at 3.0 A resolution. YqjH shares high sequence similarity with a number of known siderophore-interacting proteins and its structure was solved by molecular replacement using the siderophore-interacting protein from Shewanella putrefaciens as the search model. The YqjH structure resembles those of other members of the NAD(P)H:flavin oxidoreductase superfamily.
authors.affiliation: Structural Biology Unit, The BioCentre, University of Reading, Reading RG6 6AS, England.
authors.forename: Vicki A
authors.initials: VA
authors.lastname: Bamford
authors.forename: Maria
authors.initials: M
authors.lastname: Armour
authors.forename: Sue A
authors.initials: SA
authors.lastname: Mitchell
authors.forename: Michaël
authors.initials: M
authors.lastname: Cartron
authors.forename: Simon C
authors.initials: SC
authors.lastname: Andrews
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Watson