title: Centrin4 coordinates cell and nuclear division in T. brucei.
abstract: Centrins are Ca(2+)-binding proteins that have been implicated in a number of biological processes, including organelle duplication, mRNA export, DNA repair and signal transduction. In the protozoan parasite Trypanosoma brucei we have previously described TbCentrin2, which is present on a bi-lobed structure, and involved in the duplication and segregation of the Golgi complex. Recently, another centrin, TbCentrin4, was also found at the bi-lobe and has been implicated in organelle segregation and cytokinesis. We now show that cytokinesis is not inhibited, but that a dysregulation of nuclear and cell division leads to the production of zoids - daughter siblings that contain all organelles except the nucleus. Our results, therefore, suggest that TbCentrin4 is involved in processes that coordinate karyokinesis and cytokinesis.
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore 117543.
authors.forename: Jie
authors.initials: J
authors.lastname: Shi
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Franklin
authors.forename: Jordan T
authors.initials: JT
authors.lastname: Yelinek
authors.forename: Ingo
authors.initials: I
authors.lastname: Ebersberger
authors.forename: Graham
authors.initials: G
authors.lastname: Warren
authors.forename: Cynthia Y
authors.initials: CY
authors.lastname: He