title: [Combined transgastric and transcolonic endoscopic salpingectomy: experiment with pigs].
abstract: OBJECTIVE
  To compare the feasibility and safety of combined transgastric and transcolonic dual approach and those of transgastric single approach in endoscopic salpingectomy.
  
  
  METHODS
  Two female Chinese Nongda miniature pigs underwent gastric and colonic lavage with tap water followed by disinfection of mucosa with 1:10 iodophor. An endoscope was inserted and the colonic wall was punctured with a needle knife, dilated with a balloon-dilator and a double-channel endoscope was advanced into the peritoneal cavity. Under direct observation through this endoscope, a trans-gastric entrance was made with the second double-channel endoscope. With the help of transcolonic endoscope, the left Fallopian tube was ligated and partially resected using the transgastric endoscope. With the help of transgastric endoscope, liver biopsy was performed using the transcolonic endoscope. Finally, the gastric incision was closed with 3 clips and the colonic incision was closed with a loop and a clip. Antibiotics were used for 3 days following the procedures. Seventeen days later laparotomy was performed to observe the infection, visceral damage and adhesion, healing of the incisions of gastrointestinal duct, etc.
  
  
  RESULTS
  Compared with the single route, the dual routes were more convenient to perform the liver biopsy and salpingectomy. The pigs drank and ate normally soon after the resuscitation. The pigs looked well and gained weight during 2 weeks after the operation. Repeat endoscopy in 2 weeks showed a well-healed gastric incision with 2 clips still in place and a healed colonic incision with 1 clip still attached. The necropsy revealed a complete transmural healing of the gastric incision with minimal adhesion and a complete healing of the colonic incision without any adhesion. Few adhesions were found around the liver biopsy site and the salpingectomy site without any intraperitoneal infection or organ damage.
  
  
  CONCLUSIONS
  Combined transgastric and transcolonic approach appears safe and feasible and facilitates translumenal intraperitoneal interventions.
authors.affiliation: Department of Gastroenterology and Hepatology, General Hospital of the Chinese People's Liberation Army, Beijing 100853, China. liwen200063@sohu.com
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Sun
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Wang
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Xiao
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Sun
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Huang
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Kantsevoy