Nocardia altamirensis sp. nov., isolated from Altamira cave, Cantabria, Spain.
A novel actinomycete strain, OFN S17(T), was isolated from a sample collected from Altamira Cave, Cantabria, Spain. This strain was identified by using a polyphasic taxonomic approach. The 16S rRNA, hsp65 and sod gene sequences of the strain were determined and compared with those of representative Nocardia species. The results showed that strain OFN S17(T) should be assigned to the genus Nocardia. Phylogenetic analysis indicated that strain OFN S17(T) was most closely related to the type strain of Nocardia tenerifensis (98.6, 96.2 and 96% similarity, respectively, for the 16S rRNA, hsp65 and sod gene sequences). The DNA G+C content was 64.4 mol%. DNA-DNA hybridization analyses revealed 29% relative reassociation between the DNA of strain OFN S17(T) and N. tenerifensis DSM 44704(T). The phenotypic and genotypic data show that strain OFN S17(T) merits recognition as a representative of a novel species of the genus Nocardia, for which the name Nocardia altamirensis sp. nov. is proposed. The type strain is OFN S17(T) (=CIP 109606(T) =DSM 44997(T)).
Jurado
Valme
V
Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, E-41080 Sevilla, Spain.
Boiron
Patrick
P
Kroppenstedt
Reiner M
RM
Laurent
Frédéric
F
Couble
Andrée
A
Laiz
Leonila
L
Klenk
Hans-Peter
HP
González
Juan M
JM
Saiz-Jimenez
Cesareo
C
Mouniée
Delphine
D
Bergeron
Emanuelle
E
Rodríguez-Nava
Verónica
V