title: Chlamydophila felis CF0218 is a novel TMH family protein with potential as a diagnostic antigen for diagnosis of C. felis infection.
abstract: Chlamydophila felis is a causative agent of acute and chronic conjunctivitis and pneumonia in cats (feline chlamydiosis). Also, C. felis is a suspected zoonotic agent of such diseases as non-Chlamydia trachomatis conjunctivitis in humans, although this is controversial. At present, there is no serodiagnostic system that specifically detects C. felis infection conveniently. Current systems use antigens such as lipopolysaccharide that cross-react with all chlamydia species. In addition, it is difficult to distinguish between cats that are vaccinated with the commercial vaccine against C. felis and cats that are infected with C. felis. Here, we describe a new candidate diagnostic antigen for diagnosis of C. felis infection, CF0218, that was obtained by screening a genomic expression library of C. felis Fe/C-56 with C. felis-immunized serum. CF0218 was a putative transmembrane head (TMH) family protein with bilobed hydrophobic motifs at its N terminus, and orthologues of CF0218 were not found in the Chlamydophila pneumoniae or Chlamydia trachomatis genomes. The recombinant CF0218 was not recognized by antiserum against C. trachomatis, suggesting that CF0218 is C. felis specific. CF0218 transcription during the course of C. felis infection was confirmed by reverse transcription-PCR. By indirect immunofluorescence analysis, CF0218 was colocalized with the C. felis-formed inclusion bodies in the infected cells. The antibody response against CF0218 was elevated following C. felis infection but not by vaccination in experimentally vaccinated and infected cats. These results suggest that CF0218, a novel TMH family protein of C. felis, possesses potential as a C. felis infection-specific diagnostic antigen.
authors.affiliation: Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Ohya
authors.forename: Yu
authors.initials: Y
authors.lastname: Takahara
authors.forename: Etsuko
authors.initials: E
authors.lastname: Kuroda
authors.forename: Saori
authors.initials: S
authors.lastname: Koyasu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Hagiwara
authors.forename: Maki
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Hisaka
authors.forename: Kazuko
authors.initials: K
authors.lastname: Morizane
authors.forename: Shinryou
authors.initials: S
authors.lastname: Ishiguro
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Hideto
authors.initials: H
authors.lastname: Fukushi