title: Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain.
abstract: Avian infectious bronchitis virus (IBV) encodes 15 nonstructural proteins (nsps) which play crucial roles in RNA transcription and genome replication. One of them, nsp3, contains an ADRP (adenosine diphosphate-ribose-1'-phosphatase) domain which was revealed in recent studies to have ADP-ribose-1'-monophosphatase (Appr-1'-pase) activity. Appr-1'-pase catalyzes the conversion of ADP-ribose-1'-monophosphate (Appr-1'-p) to ADP-ribose in the tRNA-splicing pathway. The gene segment encoding the IBV nsp3 ADRP domain has been cloned and expressed in Escherichia coli. The protein has been crystallized and the crystals diffracted to 1.8 A resolution. They belonged to space group P1, with unit-cell parameters a = 41.1, b = 43.2, c = 48.9 A, alpha = 78.0, beta = 80.0, gamma = 73.6 degrees . Each asymmetric unit contains two molecules.
authors.affiliation: Tsinghua-Nankai-IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wei
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.forename: Le
authors.initials: L
authors.lastname: Cong
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Xu
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Ma
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xu
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao