Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray.
In flowering plants, the male gametophyte, the pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. Expression profiles of 11 known tapetum specific-genes were consistent with previous reports. Based on their spatial and temporal expression patterns, 140 genes which had been previously defined as anther specific were further classified as male gametophyte specific (71 genes, 51%), tapetum-specific (seven genes, 5%) or expressed in both male gametophyte and tapetum (62 genes, 44%). These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum.
Suwabe
Keita
K
Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.
Suzuki
Go
G
Takahashi
Hirokazu
H
Shiono
Katsuhiro
K
Endo
Makoto
M
Yano
Kentaro
K
Fujita
Masahiro
M
Masuko
Hiromi
H
Saito
Hiroshi
H
Fujioka
Tomoaki
T
Kaneko
Fumi
F
Kazama
Tomohiko
T
Mizuta
Yoko
Y
Kawagishi-Kobayashi
Makiko
M
Tsutsumi
Nobuhiro
N
Kurata
Nori
N
Nakazono
Mikio
M
Watanabe
Masao
M