title: Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor.
abstract: We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd2+ levels with the use of two excited wavelengths.
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. taki@chem.mbox.media.kyoto-u.ac.jp
authors.forename: Masayasu
authors.initials: M
authors.lastname: Taki
authors.forename: Mika
authors.initials: M
authors.lastname: Desaki
authors.forename: Akio
authors.initials: A
authors.lastname: Ojida
authors.forename: Shohei
authors.initials: S
authors.lastname: Iyoshi
authors.forename: Tasuku
authors.initials: T
authors.lastname: Hirayama
authors.forename: Itaru
authors.initials: I
authors.lastname: Hamachi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamamoto