title: Cloning and characterization of a novel member of human beta-1,4-galactosyltransferase gene family.
abstract: By using the EST strategy for identifying novel members belonging to homologous gene families, a novel fulklength cDNA encoding a protein significantly homologous to UDP-Gal: N-acetylglucosamine beta-1, 4-galactosyltransferase (GalT) was isolated from a human testis cDNA library. A nucleotide sequence of 2 173 bp long was determined to contain an open reading frame of 1 032 nucleotides (344 amino acids). In view of the homology to memben of the galactosyltransferase gene family and especially the closest relationship toGallus gallus GalT type I (CK I), the predicted product of the novel cDNA was designated as human beta-1,4-galactosyltransferase homolog I (HumGT-H1). Its mRNA is present in different degrees in 16 tissues examined. Southern analysis of human genomic DNA revealed its locus on chromosome 3.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, 200433, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Fan
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: F
authors.initials: F
authors.lastname: Dai
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Tu
authors.forename: A
authors.initials: A
authors.lastname: Bi
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: S
authors.initials: S
authors.lastname: Zhao