title: [Construction and identification of Ksp-cadherin-Gpx1-Klk1 expression vector].
abstract: OBJECTIVE
  To construct a Gpx1 and klk1 recombinant vector containing the kidney-specific promoter Ksp-cadherin.
  
  
  METHODS
  Human Gpx1, Klk1 and Ksp-cadherin cDNAs were amplified with PCR and inserted in a stepwise manner into the expressive vector pIRES-EGFP to construct the recombinant vector Ksp-cadherin-Gpx1-Klk1. The constructed vector was verified with restriction enzyme digestion and sequence analysis.
  
  
  CONCLUSIONS
  The recombinant expression vector Ksp-cadherin-Gpx1-Klk1 was constructed and identified successfully, which provides a potent tool for preparing transgenic animals to investigate gene therapy for ischemia-reperfusion injury in kidney transplantation.
authors.affiliation: Department of Kidney Transplantation, First Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, China. xieliyi0313@163.com
authors.forename: Li-yi
authors.initials: LY
authors.lastname: Xie
authors.forename: Wu-jun
authors.initials: WJ
authors.lastname: Xue
authors.forename: He-li
authors.initials: HL
authors.lastname: Xiang
authors.forename: Sun-kai
authors.initials: SK
authors.lastname: Ma