Genomic, genetic and physiological effects of bio-electrospraying on live cells of the model yeast Saccharomyces cerevisiae.
The ability to directly engineer living cells is rapidly becoming a hot field of research for a wide range of applications within the life sciences. 'Bio-electrospraying' cells, a recently developed technique, has great potential in this area. In this paper, we quantify genetic, genomic and physiological effects of bio-electrospraying cells of a model eukaryote, the yeast Saccharomyces cerevisiae. Our results demonstrate that yeast cells bio-electrosprayed at 30 kV have not incurred any detectable damage at a genomic or genetic level, and that the detectable physiological stress of the procedure is negligible. These results support our proposal to use yeast as a model system to develop bio-electrospray devices and protocols.
Greig
Duncan
D
Department of Biology, University College London, Gower Street, London WC1E 6BT, UK.
Jayasinghe
Suwan N
SN