Proteome analysis identifies the role of heat stress in production of progeny HCV in Huh7 cells harboring intact HCV.
OBJECTIVE
The intact hepatitis C virus (HCV) cell culture system has provided a powerful tool for studying the interaction between HCV and host cell. We applied proteomic techniques to globally analyze the protein expression profiles of Huh7 in the absence and presence of HCV, with the aim to elucidate the host cell components response to HCV.


METHODS
Proteomic and molecular biology techniques were used for this aim.


RESULTS
The expression of many proteins, including regucalcin, centriolin and several keratins, was up-regulated, after HCV transfection. Among the down-regulated expression proteins, the heat-shock protein family was of prime significance. Subsequently, we studied the role of heat stress in the interaction between HCV and host cell, based on proteomics analysis. We found heat stress did not affect the production of HCV in this in vitro cell culture system.


CONCLUSIONS
Moreover, this study also provided the global information of proteomic alteration of Huh7 cells in the presence of intact HCV and further improved the understanding of the mechanism of interaction between HCV and host cell. We also draw a conclusion that heat stress had no affect on the production of HCV.
Zhao
Sihai
S
Department of Immunology and Microbiology, Xi'an Jiaotong University School of Medicine, Xi'an, China.
Xun
Meng
M
Chu
Yonglie
Y
Zhu
Tong
T
Wang
Yihua
Y
Yan
Lipeng
L
Xun
Xin
X
Xu
Kun
K
Song
Juan
J
Shao
Mingming
M