title: [Ferrous-manganese oxidizing bacteria from the nature water].
abstract: Glass slides were hanged into a canal to acquire the ferrous-manganese oxidizing bacteria settled bio-film. Two isolated methods for ferrous-manganese oxidizing bacteria with special iron-manganese oxidizing matrix from the bio-film were tested. Element component of bacteria product and sheath structure of bacteria were analyzed. With two methods, plate cultivation and the novel semi-solid in situ cultivation method, strains belong to Family Leptothrix were isolated. XRF showed that the amorphous iron and manganese were two major metal elements of the precipitation formed by one strain of Leptothrix spp.. Through the microscope observation, one strain of Family Leptothrix was determined to form branch-like structured sheath, while another strain formed spider web-like structured sheath. Those isolated bacteria provide model strains for future testing of FISH probe and PCR primer of ferrous-manganese oxidizing bacteria.
authors.affiliation: Key Laboratory of Water Resources Utilization and Environmental Pollution Control, Harbin Institute of Technology, Harbin 150090, China. qinsongyan@yahoo.com.cn
authors.forename: Song-yan
authors.initials: SY
authors.lastname: Qin
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.forename: Peng
authors.initials: P
authors.lastname: Huang