KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate.
A novel gene, bla(KHM-1), encoding a metallo-beta-lactamase, KHM-1, was cloned from a clinical isolate of Citrobacter freundii resistant to most beta-lactam antibiotics. Escherichia coli expressing bla(KHM-1) was resistant to all broad-spectrum beta-lactams except for monobactams and showed reduced susceptibility to carbapenems. Recombinant KHM-1 exhibited EDTA-inhibitable hydrolytic activity against most beta-lactams, with an overall preference for cephalosporins.
Sekiguchi
Jun-ichiro
J
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Shinjuku, Tokyo, Japan.
Morita
Koji
K
Kitao
Tomoe
T
Watanabe
Noboru
N
Okazaki
Mitsuhiro
M
Miyoshi-Akiyama
Tohru
T
Kanamori
Masato
M
Kirikae
Teruo
T