A simple, sensitive, specific detection of mixed infection of multiple plant viruses using macroarray and microtube hybridization.
A simple, sensitive and specific method using a cDNA macroarray to detect multiple viruses was devised. The method is used in plants such as potato and lily, which need a reliable routine diagnosis for mixed infection. The biotinylated cRNA targets were prepared using an in vitro transcription-based system that was designed especially to eliminate nonspecific hybridizations. The macroarray hybridization was carried out using a convenient, cost-effective "microtube hybridization" (MTH) system. By this method, lily viruses including Cucumber mosaic virus, Lily symptomless virus, Lily mottle virus, and Plantago asiatica mosaic virus were detected successfully from leaves or roots of lily bulbs.
Sugiyama
Shunpei
S
Lab Company Limited, Kita-ku, Kita 27, Nishi 6, Sapporo 001-0027, Japan.
Masuta
Chikara
C
Sekiguchi
Hiroshi
H
Uehara
Taketo
T
Shimura
Hanako
H
Maruta
Yukio
Y