title: Proteomic analysis of anti-tumor effects by Rhizoma Paridis total saponin treatment in HepG2 cells.
abstract: Rhizoma Paridis total saponin (RPTS) had been identified as the major components responsible for the anti-tumor effects of the herb Rhizoma Paridis, which had been used in China for centuries to treat many diseases including tumor. To elucidate the anti-tumor mechanism of RPTS, a proteomic analysis was carried out with RPTS treatment in HepG2 cells. More than 50 proteins showed a significant change between control (0.01% DMSO) and RPTS (IC(50) approximately 10microg/ml) treated cells after 48h. Twelve proteins had been identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) using peptide fingerprinting from 15 protein spots (density difference >2 fold between the control and RPTS-treated group). Among them, six proteins were down-regulated (dUTPase, hnRNP K, GMP synthase, etc.) and six proteins were up-regulated (DNase gamma, Nucleoside diphosphate kinase A, Centrin-2, etc.) by RPTS treatment in HepG2 cells as determined by spot volume (p<0.05). Most of the identified proteins were associated with tumor initiation, promotion, and progression. These findings might offer valuable insights into the mechanism of anti-tumor effect affected by RPTS treatment in HepG2 cells.
authors.affiliation: Department of Oncology, Drum Tower Medical School of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Cheng
authors.forename: Bao-Rui
authors.initials: BR
authors.lastname: Liu
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Qian
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Ding
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Hu
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Yu