title: Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states.
abstract: Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca(2+) together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.
authors.affiliation: Laboratory of Neurobiology, Shanghai Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wei
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Cheng
authors.forename: Keying
authors.initials: K
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Minqian
authors.initials: M
authors.lastname: Li
authors.forename: Peihong
authors.initials: P
authors.lastname: Zhu