title: Therapeutic effect against human xenograft tumors in nude mice by the third generation microtubule stabilizing epothilones.
abstract: The epothilones represent a promising class of natural product-based antitumor drug candidates. Although these compounds operate through a microtubule stabilization mechanism similar to that of taxol, the epothilones offer a major potential therapeutic advantage in that they retain their activity against multidrug-resistant cell lines. We have been systematically synthesizing and evaluating synthetic epothilone congeners that are not accessible through modification of the natural product itself. We report herein the results of biological investigations directed at two epothilone congeners: iso-fludelone and iso-dehydelone. Iso-fludelone, in particular, exhibits a number of properties that render it an excellent candidate for preclinical development, including biological stability, excellent solubility in water, and remarkable potency relative to other epothilones. In nude mouse xenograft settings, iso-fludelone was able to achieve therapeutic cures against a number of human cancer cell lines, including mammarian-MX-1, ovarian-SK-OV-3, and the fast-growing, refractory, subcutaneous neuroblastoma-SK-NAS. Strong therapeutic effect was observed against drug-resistant lung-A549/taxol and mammary-MCF-7/Adr xenografts. In addition, iso-fludelone was shown to exhibit a significant therapeutic effect against an intracranially implanted SK-NAS tumor.
authors.affiliation: Preclinical Pharmacology Laboratory, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.
authors.forename: Ting-Chao
authors.initials: TC
authors.lastname: Chou
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Zhang
authors.forename: Zi-Yang
authors.initials: ZY
authors.lastname: Zhong
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Sara
authors.initials: S
authors.lastname: Eng
authors.forename: David R
authors.initials: DR
authors.lastname: Myles
authors.forename: Robert
authors.initials: R
authors.lastname: Johnson
authors.forename: Nian
authors.initials: N
authors.lastname: Wu
authors.forename: Ye Ingrid
authors.initials: YI
authors.lastname: Yin
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Wilson
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Danishefsky