title: Application of monoclonal antibodies in the Dutch avian leukosis control programme.
abstract: Comparative testing with series of albumen samples obtained from seven Dutch poultry flocks was performed for avian leukosis virus group-specific antigen (ALV-gsa) by complement fixation test (CFT) and by a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) employing selected monoclonal antibodies directed to different ALV-p27 epitopes. The higher sensitivity for ALV-gsa detection of the DAS-ELISA resulted in increased scores of ALV-shedding hens in all seven flocks. Relatively low absorbance values seem to be associated with low rates of congenital ALV transmission.
authors.affiliation: Virology Department, Central Veterinary Institute, Lelystad, The Netherlands.
authors.forename: G F
authors.initials: GF
authors.lastname: De Boer
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: B
authors.initials: B
authors.lastname: Kouwenhoven