title: Cutting edge: instructive role of peripheral tissue cells in the imprinting of T cell homing receptor patterns.
abstract: Tissue-specific homing of effector and memory T cells to skin and small intestine requires the imprinting of specific combinations of adhesion molecules and chemokine receptors by dendritic cells in the draining lymph nodes. In this study, we demonstrate that CD8(+) T cells activated by Ag-pulsed bone marrow-derived dendritic cells were induced to express the small intestine homing receptors alpha(4)beta(7) integrin and chemokine receptor CCR9 in coculture with small intestinal epithelial cells. In contrast, in coculture with dermal fibroblasts the skin-homing receptor E-selectin ligand was induced. Interestingly, the imprinting of gut homing receptors on anti-CD3/anti-CD28 stimulated T cells was induced by soluble factors produced by small intestinal epithelial cells. Retinoic acid was identified as a crucial factor. These findings show that peripheral tissue cells directly produce homing receptor imprinting factors and suggest that dendritic cells can acquire their imprinting potential already in the peripheral tissue of origin.
authors.affiliation: Allergy Research Group, Department of Dermatology, University Medical Center, University of Freiburg, Freiburg, Germany.
authors.forename: Fanny
authors.initials: F
authors.lastname: Edele
authors.forename: Rosalie
authors.initials: R
authors.lastname: Molenaar
authors.forename: Dominique
authors.initials: D
authors.lastname: Gütle
authors.forename: Jan C
authors.initials: JC
authors.lastname: Dudda
authors.forename: Thilo
authors.initials: T
authors.lastname: Jakob
authors.forename: Bernhard
authors.initials: B
authors.lastname: Homey
authors.forename: Reina
authors.initials: R
authors.lastname: Mebius
authors.forename: Mathias
authors.initials: M
authors.lastname: Hornef
authors.forename: Stefan F
authors.initials: SF
authors.lastname: Martin