title: Occurrence and characteristics of the cytolysin A gene in Shigella strains and other members of the family Enterobacteriaceae.
abstract: Cytolysin A (ClyA, HlyE, SheA) is a hemolytic pore-forming toxin found in Escherichia coli and Salmonella enterica serovars Typhi and Paratyphi A. In the present study, analysis of several Shigella strains revealed that they harbor only nonfunctional clyA gene copies that have been inactivated either by the integration of insertion sequence (IS) elements (Shigella dysenteriae, Shigella boydii, and Shigella sonnei strains) or by a frameshift mutation (Shigella flexneri). Shigella dysenteriae and S. boydii strains also exhibited IS-associated deletions at the clyA locus. PCR and Southern blot analyses as well as database searches indicated that clyA-related DNA sequences are completely absent in strains belonging to various other genera of the family Enterobacteriaceae. According to these data, ClyA may play a role only for a rather small subset of the enteric bacteria.
authors.affiliation: Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Klinikum der Johann Wolfgang Goethe-Universität, Paul-Ehrlich-Strasse 40, Frankfurt am Main, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: von Rhein
authors.forename: Susanne
authors.initials: S
authors.lastname: Bauer
authors.forename: Valeska
authors.initials: V
authors.lastname: Simon
authors.forename: Albrecht
authors.initials: A
authors.lastname: Ludwig