title: Crystallographic study of G178S mutant of human proliferating cell nuclear antigen.
abstract: Proliferating cell nuclear antigen (PCNA) is an evolutionarily conserved protein that forms a ring-shaped homotrimer that functions as a sliding clamp for DNA replication. The rev6-1 mutation of Saccharomyces cerevisiae, which inactivates both translesion DNA synthesis and damage-avoidance pathways while having little effect on normal cell growth, has a G178S substitution in the PCNA protein. Human PCNA protein carrying the G178S substitution was crystallized. Two crystal forms were obtained under similar conditions. Crystal forms I and II belong to space groups P2(1), with unit-cell parameters a = 84.1, b = 130.2, c = 97.8 A, beta = 113.4 degrees , and P2(1)2(1)2(1), with unit-cell parameters a = 68.1, b = 100.2, c = 131.2 A, respectively. Structural analyses by molecular replacement are now in progress.
authors.affiliation: International Graduate School of Arts and Sciences, Yokohama City University, Japan.
authors.forename: Asami
authors.initials: A
authors.lastname: Hishiki
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Aya
authors.initials: A
authors.lastname: Serizawa
authors.forename: Haruo
authors.initials: H
authors.lastname: Ohmori
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto