Expression of recombinant human nerve growth factor beta in milk of goats by recombinant replication-defective adenovirus.
For producing a large quantity of human nerve growth factor beta (hNGF-beta), the recombinant adenovirus containing an expression cassette of hNGF-beta complementary DNA gene was constructed and then instilled directly into goats' mammary glands. The recombinant hNGF-beta was detected in the milk. The maximal expression level of recombinant hNGF-beta in the milk reached 196.8 mg/l. The biological activity of the recombinant hNGF-beta, compared with the commercial hNGF-beta, was tested using PC12 cells. The results demonstrate that instilling recombinant adenovirus directly into the mammary gland of goat is an efficient approach to producing a large quantity of hNGF-beta.
Xiao
Bo
B
College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Li
Qingwang
Q
Feng
Bo
B
Han
Zengsheng
Z
Gao
Dawei
D
Zhao
Rui
R
Li
Jian
J
Li
Kun
K
Zhi
Xubo
X
Yang
Hai
H
Liu
Zhibin
Z