title: Novel aldosterone synthase inhibitors with extended carbocyclic skeleton by a combined ligand-based and structure-based drug design approach.
abstract: Pharmacophore modeling of a series of aldosterone synthase (CYP11B2) inhibitors triggered the design of compounds 11 and 12 by extending a previously established naphthalene molecular scaffold (e.g., present in molecules 1 and 2) via introduction of a phenyl or benzyl residue in 3-position. These additional aromatic moieties have been hypothesized to fit into the newly identified hydrophobic pharmacophore feature HY3. Subsequent docking studies in our refined CYP11B2 protein model have been performed prior to synthesis to estimate the inhibitory properties of the proposed molecules. While phenyl-substituted compound 11 (IC50 > 500 nM) did not dock under the given pharmacophore constraint (i.e., the Fe(heme)-N(ligand) interaction), benzyl-substituted compound 12 (IC50 = 154 nM) was found to exploit a previously unexplored subpocket of the inhibitor binding site. By structural optimization based on the pharmacophore hypothesis, 25 novel compounds were synthesized, among them highly potent CYP11B2 inhibitors (e.g., 17, IC50 = 2.7 nM) with pronounced selectivity toward the most important steroidogenic and hepatic CYP enzymes.
authors.affiliation: Pharmaceutical and Medicinal Chemistry, Saarland University, Saarbrucken, Germany.
authors.forename: Simon
authors.initials: S
authors.lastname: Lucas
authors.forename: Ralf
authors.initials: R
authors.lastname: Heim
authors.forename: Matthias
authors.initials: M
authors.lastname: Negri
authors.forename: Iris
authors.initials: I
authors.lastname: Antes
authors.forename: Christina
authors.initials: C
authors.lastname: Ries
authors.forename: Katarzyna E
authors.initials: KE
authors.lastname: Schewe
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bisi
authors.forename: Silvia
authors.initials: S
authors.lastname: Gobbi
authors.forename: Rolf W
authors.initials: RW
authors.lastname: Hartmann