[High-performance capillary electrophoresis for determining the chemical compositions of Gegen and Fenge extracts].
OBJECTIVE
To develop a method for analyzing the chemical compositions of Gegen and Fenge extracts using high-performance capillary electrophoresis (HPCE).


METHODS
Using HPCE/DAD, the chemical composition of the extracts was analyzed with the buffer solution of 40 mmol/L borax containing 16.7% methanol, with injection pressure at 137.9 kPa for 5 s, separation voltage at 25 kV in 0-5 min time range and at 22 kV in 5-25 min time range, and the temperature of the capillary of 20 degrees celsius.


CONCLUSIONS
The method for analysis of Gegen and Fenge extracts was established, which identified puerarin and daidzein as the two major components. This simple and rapid analysis method can be used for Gegen and Fenge extract fingerprinting.
Wang
Yong-gang
YG
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China. awag7612@yahoo.com.cn
Sun
Xue-gang
XG
Wei
Feng-huan
FH