title: Arrestin 3 mediates endocytosis of CCR7 following ligation of CCL19 but not CCL21.
abstract: Internalization of ligand bound G protein-coupled receptors, an important cellular function that mediates receptor desensitization, takes place via distinct pathways, which are often unique for each receptor. The C-C chemokine receptor (CCR7) G protein-coupled receptor is expressed on naive T cells, dendritic cells, and NK cells and has two endogenous ligands, CCL19 and CCL21. Following binding of CCL21, 21 +/- 4% of CCR7 is internalized in the HuT 78 human T cell lymphoma line, while 76 +/- 8% of CCR7 is internalized upon binding to CCL19. To determine whether arrestins mediated differential internalization of CCR7/CCL19 vs CCR7/CCL21, we used small interfering RNA (siRNA) to knock down expression of arrestin 2 or arrestin 3 in HuT 78 cells. Independent of arrestin 2 or arrestin 3 expression, CCR7/CCL21 internalized. In contrast, following depletion of arrestin 3, CCR7/CCL19 failed to internalize. To examine the consequence of complete loss of both arrestin 2 and arrestin 3 on CCL19/CCR7 internalization, we examined CCR7 internalization in arrestin 2(-/-)/arrestin 3(-/-) murine embryonic fibroblasts. Only reconstitution with arrestin 3-GFP but not arrestin 2-GFP rescued internalization of CCR7/CCL19. Loss of arrestin 2 or arrestin 3 blocked migration to CCL19 but had no effect on migration to CCL21. Using immunofluorescence microscopy, we found that arrestins do not cluster at the membrane with CCR7 following ligand binding but cap with CCR7 during receptor internalization. These are the first studies that define a role for arrestin 3 in the internalization of a chemokine receptor following binding of one but not both endogenous ligands.
authors.affiliation: Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, KS 66160, USA.
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Byers
authors.forename: Psachal A
authors.initials: PA
authors.lastname: Calloway
authors.forename: Laurie
authors.initials: L
authors.lastname: Shannon
authors.forename: Heather D
authors.initials: HD
authors.lastname: Cunningham
authors.forename: Sarah
authors.initials: S
authors.lastname: Smith
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Brian C
authors.initials: BC
authors.lastname: Fassold
authors.forename: Charlotte M
authors.initials: CM
authors.lastname: Vines
mesh_terms: D000818:Animals; D019390:Arrestins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D054415:Chemokine CCL19 / Q000378:metabolism*; D054421:Chemokine CCL21 / Q000378:metabolism; D004705:Endocytosis / Q000276:immunology*; D006801:Humans; D019169:Jurkat Cells; D008024:Ligands; D051379:Mice; D011485:Protein Binding / Q000276:immunology; D034741:RNA, Small Interfering / Q000502:physiology; D011940:Receptor Aggregation / Q000276:immunology; D054400:Receptors, CCR7 / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D000071557:beta-Arrestins
chemical_list: D019390:Arrestins; C515460:CCL19 protein, human; C515478:CCL21 protein, human; C515370:CCR7 protein, human; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D008024:Ligands; D034741:RNA, Small Interfering; D054400:Receptors, CCR7; C082333:arrestin3; D000071557:beta-Arrestins
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