title: Isolation and spectral characterization of Photosystem II reaction center from Synechocystis sp. PCC 6803.
abstract: We isolated highly-purified photochemically active photosystem (PS) II reaction center (RC) complexes from the cyanobacterium Synechocystis sp. PCC 6803 using a histidine-tag introduced to the 47 kDa chlorophyll protein, and characterized their spectroscopic properties. Purification was carried out in a one-step procedure after isolation of PS II core complex. The RC complexes consist of five polypeptides, the same as in spinach. The pigment contents per two molecules of pheophytin a were 5.8 +/- 0.3 chlorophyll (Chl) a and 1.8 +/- 0.1 beta-carotene; one cytochrome b(559) was found per 6.0 Chl a molecules. Overall absorption and fluorescence properties were very similar to those of spinach PS II RCs; our preparation retains the best properties so far isolated from cyanobacteria. However, a clear band-shift of pheophytin a and beta-carotene was observed. Reasons for these differences, and RC composition, are discussed on the basis of the three-dimensional structure of complexes.
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. tomo@z05.mbox.media.kyoto-u.ac.jp
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tomo
authors.forename: Seiji
authors.initials: S
authors.lastname: Akimoto
authors.forename: Tohru
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Michitaka
authors.initials: M
authors.lastname: Fukuya
authors.forename: Kazunori
authors.initials: K
authors.lastname: Tanaka
authors.forename: Mamoru
authors.initials: M
authors.lastname: Mimuro
mesh_terms: D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D003573:Cytochrome b Group / Q000032:analysis; D010674:Pheophytins / Q000032:analysis; D045332:Photosystem II Protein Complex / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence; D046939:Synechocystis / Q000737:chemistry*; D019207:beta Carotene / Q000032:analysis
chemical_list: D003573:Cytochrome b Group; D010674:Pheophytins; D045332:Photosystem II Protein Complex; D019207:beta Carotene; D002734:Chlorophyll; C061694:pheophytin a; C019313:cytochrome b559; D000077194:Chlorophyll A
references.citation: FEBS Lett. 1988 Dec 5;241(1-2):99-104
references.pmid: 3058517
references.citation: Plant Physiol. 2007 Jul;144(3):1336-46
references.pmid: 17513482
references.citation: Biochim Biophys Acta. 1973 Jun 28;305(3):597-609
references.pmid: 4354794
references.citation: Biochemistry. 2002 Jun 25;41(25):8004-12
references.pmid: 12069591
references.citation: Photosynth Res. 2001;67(3):199-206
references.pmid: 16228307
references.citation: Biochemistry. 2008 Mar 11;47(10):3143-54
references.pmid: 18278871
references.citation: Biochemistry. 2000 Nov 28;39(47):14583-94
references.pmid: 11087414
references.citation: Biochim Biophys Acta. 1998 Nov 2;1409(1):50-7
references.pmid: 9804889
references.citation: Biochim Biophys Acta. 2007 Apr;1767(4):327-34
references.pmid: 17382895
references.citation: J Biol Chem. 2004 Jul 9;279(28):29622-7
references.pmid: 15123635
references.citation: Biochemistry. 2001 Feb 20;40(7):2176-85
references.pmid: 11329286
references.citation: Biochemistry. 2003 Aug 5;42(30):9205-13
references.pmid: 12885255
references.citation: Nature. 2005 Dec 15;438(7070):1040-4
references.pmid: 16355230
references.citation: Photosynth Res. 2005 Jun;84(1-3):77-83
references.pmid: 16049758
references.citation: Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):98-103
references.pmid: 12518057
references.citation: J Biol Chem. 1996 Jan 26;271(4):2093-101
references.pmid: 8567665
references.citation: Proc Natl Acad Sci U S A. 1987 Aug;84(16):5730-4
references.pmid: 3303032
references.citation: Photosynth Res. 2002;72(2):175-89
references.pmid: 16228516
references.citation: Philos Trans R Soc Lond B Biol Sci. 2002 Oct 29;357(1426):1431-39; discussion 1439-40, 1469-70
references.pmid: 12437882
references.citation: Biochemistry. 1990 Nov 13;29(45):10376-87
references.pmid: 2261479
references.citation: J Mol Biol. 1995 Feb 24;246(3):429-57
references.pmid: 7877166
references.citation: Science. 2004 Mar 19;303(5665):1831-8
references.pmid: 14764885
references.citation: FEBS Lett. 2004 Jan 2;556(1-3):95-8
references.pmid: 14706833
references.citation: Biochemistry. 2001 Mar 27;40(12):3690-9
references.pmid: 11297437
references.citation: Biochemistry. 2003 Feb 4;42(4):1008-15
references.pmid: 12549921
references.citation: Biochemistry. 2007 Apr 10;46(14):4390-7
references.pmid: 17371054
references.citation: J Plant Physiol. 2003 Jan;160(1):41-9
references.pmid: 12685044
references.citation: Proc Natl Acad Sci U S A. 1987 Jan;84(1):109-12
references.pmid: 16593792
references.citation: Photosynth Res. 2007 Nov-Dec;94(2-3):183-202
references.pmid: 17634752
references.citation: FEBS Lett. 1990 Jun 4;265(1-2):88-92
references.pmid: 2194834
references.citation: FEBS Lett. 1994 Feb 14;339(1-2):25-30
references.pmid: 8313975
references.citation: Biochemistry. 1995 Apr 25;34(16):5523-6
references.pmid: 7727412
references.citation: Biochim Biophys Acta. 1999 May 26;1412(1):37-46
references.pmid: 10354492