Analysis of modified nucleosides in the urine of patients with malignant cancer by liquid chromatography/electrospray ionization mass spectrometry.
As modified nucleosides reflect altered tRNA turnover which seems to be impaired in the body of cancer patients, they have been evaluated as potential tumor markers. High-performance liquid chromatography/electrosprary ionization quadrupole time-of-flight mass spectrometry (HPLC/ESI-Q-TOFMS) was used to identify nucleosides purified from urine in positive ionization mode. Potential nucleosides were assessed by their evident UV absorbance in HPLC and then further examined by mass spectrometric techniques. In this manner, 21 nucleosides were detected in the urine of a patient with lymphoid cancer including three modified nucleosides 5'-dehydro-2-deoxyinosine, N1,N2,N7-trimethylguanosine and N1-methyl-N2-ethylguanosine, which had never been reported previously.
Li
Hua-Yu
HY
Department of Chemistry, Zhengzhou University, 100 Science Road, 450001 Zhengzhou, China.
Wang
Shao-Min
SM
Liu
Hong-Min
HM
Li
Juan
J
Han
Dong
D
Bu
Shan-Shan
SS
Zhang
Ming-Zhi
MZ
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D009369:Neoplasms / Q000652:urine*; D009705:Nucleosides / Q000652:urine*; D012334:RNA, Neoplasm / Q000652:urine; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization
D007202:Indicators and Reagents; D009705:Nucleosides; D012334:RNA, Neoplasm