title: Calgranulin B (S100A9) levels in bronchoalveolar lavage fluid of patients with interstitial lung diseases.
abstract: Calgranulins are small calcium-binding proteins with several immunological functions involved in inflammatory processes. Calgranulin A is reported to be mainly associated with acute inflammation while calgranulin B seems to play a role in chronic inflammatory disorders. In this study we used a proteomic approach to analyse calgranulin B expression in bronchoalveolar lavage (BAL) from a group of patients with different interstitial lung diseases. Two dimensional electrophoresis analysis of BAL was performed in 11 idiopathic pulmonary fibrosis patients, nine sarcoidosis patients, 11 with systemic sclerosis patients and five healthy controls. Significantly higher (p<0.001) calgranulin B percentage volumes were observed in BAL from IPF patients than controls and other ILD patients. This result sustains the hypothesis that calgranulin B could be involved in chronic lung diseases, probably through increased expression and enhanced activation of alveolar polymorphonuclear cells related to idiopathic pulmonary fibrosis. Quantitative analysis by an easier method applied to a larger population will be necessary to determine whether calgranulin B could be a good marker of disease severity.
authors.affiliation: Sezione Malattie Respiratorie, Dipartimento di Medicina Clinica e Scienze Immunologiche, Università di Siena, Siena, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Bargagli
authors.forename: C
authors.initials: C
authors.lastname: Olivieri
authors.forename: A
authors.initials: A
authors.lastname: Prasse
authors.forename: N
authors.initials: N
authors.lastname: Bianchi
authors.forename: B
authors.initials: B
authors.lastname: Magi
authors.forename: R
authors.initials: R
authors.lastname: Cianti
authors.forename: L
authors.initials: L
authors.lastname: Bini
authors.forename: P
authors.initials: P
authors.lastname: Rottoli
mesh_terms: D000328:Adult; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D040502:Calgranulin B / Q000378:metabolism*; D005260:Female; D005355:Fibrosis; D006801:Humans; D007107:Immune System; D007249:Inflammation; D017563:Lung Diseases, Interstitial / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D012507:Sarcoidosis / Q000378:metabolism*; D012595:Scleroderma, Systemic / Q000378:metabolism*
chemical_list: D040502:Calgranulin B
references.citation: J Leukoc Biol. 2007 Jan;81(1):28-37
references.pmid: 16943388
references.citation: Proteomics. 2005 Apr;5(5):1423-30
references.pmid: 15761959
references.citation: Sarcoidosis Vasc Diffuse Lung Dis. 1999 Sep;16(2):149-73
references.pmid: 10560120
references.citation: Proteomics. 2006 Jul;6(13):3949-57
references.pmid: 16767788
references.citation: Electrophoresis. 1988 Nov;9(11):681-92
references.pmid: 3250872
references.citation: Respir Med. 2008 Apr;102(4):567-73
references.pmid: 18164192
references.citation: Immunobiology. 1992 Nov;186(3-4):304-14
references.pmid: 1490735
references.citation: Am J Physiol Lung Cell Mol Physiol. 2004 Jun;286(6):L1095-104
references.pmid: 14742308
references.citation: Orphanet J Rare Dis. 2008 Mar 26;3:8
references.pmid: 18366757
references.citation: Expert Rev Proteomics. 2008 Feb;5(1):9-12
references.pmid: 18282117
references.citation: Electrophoresis. 2002 Sep;23(19):3434-44
references.pmid: 12373774
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 5;815(1-2):169-78
references.pmid: 15652807
references.citation: Electrophoresis. 1993 Dec;14(12):1357-65
references.pmid: 8137802
references.citation: Mol Cell Proteomics. 2005 Aug;4(8):1052-60
references.pmid: 15901828
references.citation: Mol Cell Biol. 1988 Jun;8(6):2402-10
references.pmid: 3405210
references.citation: Proteomics. 2005 Jul;5(11):2972-80
references.pmid: 16075419