title: P2X receptors are differentially expressed on vasopressin- and oxytocin-containing neurons in the supraoptic and paraventricular nuclei of rat hypothalamus.
abstract: In the present study, the distribution of P2X receptor protein and colocalization of P2X receptors with vasopressin and oxytocin in the supraoptic and paraventricular nuclei of rat hypothalamus was studied using double-labeling fluorescence immunohistochemistry. The results showed that vasopressin-containing neurons expressed P2X(2), P2X(4), P2X(5) and P2X(6) receptor and oxytocin-containing neurons expressed P2X(2), P2X(4) and P2X(5) receptors in the supraoptic nucleus. In the paraventricular nucleus, vasopressin-containing neurons expressed P2X(4), P2X(5) and P2X(6) receptors, while oxytocin-containing neurons expressed P2X(4) receptors. This study provides the first evidence that P2X receptor subunits are differentially expressed on vasopressin- and oxytocin-containing neurons in the supraoptic and paraventricular nuclei, and hence, provides a substantial neuroanatomical basis for possible functional interactions between the purinergic and vasopressinergic systems, and the purinergic and oxytocinergic systems in the rat hypothalamus.
authors.affiliation: Department of Neurobiology, Second Military Medical University, 200433, Shanghai, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Jihu
authors.initials: J
authors.lastname: Sun
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Xu
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Bunstock
authors.forename: Cheng
authors.initials: C
authors.lastname: He
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Xiang
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D010121:Oxytocin / Q000032:analysis* / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000032:analysis / Q000378:metabolism*; D058469:Receptors, Purinergic P2X; D013495:Supraoptic Nucleus / Q000378:metabolism*; D014667:Vasopressins / Q000032:analysis* / Q000378:metabolism
chemical_list: D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D014667:Vasopressins; D010121:Oxytocin
references.citation: Cell Mol Life Sci. 2007 Jun;64(12):1471-83
references.pmid: 17375261
references.citation: Neuroscience. 1980;5(7):1351-6
references.pmid: 7402474
references.citation: Neuroscience. 2003;121(2):411-9
references.pmid: 14521999
references.citation: Physiol Rev. 2007 Apr;87(2):659-797
references.pmid: 17429044
references.citation: Exp Physiol. 2006 Mar;91(2):403-11
references.pmid: 16299016
references.citation: Physiol Rev. 2002 Oct;82(4):1013-67
references.pmid: 12270951
references.citation: Mol Pharmacol. 1995 Oct;48(4):569-73
references.pmid: 7476880
references.citation: Brain Res. 2006 Jul 12;1099(1):56-63
references.pmid: 16765918
references.citation: Trends Pharmacol Sci. 2006 Mar;27(3):166-76
references.pmid: 16487603
references.citation: Br J Pharmacol. 2002 Apr;135(8):2047-55
references.pmid: 11959809
references.citation: J Endocrinol. 1999 Mar;160(3):343-50
references.pmid: 10076181
references.citation: J Neurocytol. 1999 Jun;28(6):495-504
references.pmid: 10767101
references.citation: Brain Res. 1998 Dec 7;813(2):390-7
references.pmid: 9838201
references.citation: Brain Res. 1994 Apr 4;641(2):249-56
references.pmid: 8012826
references.citation: J Neurosci. 2000 Dec 1;20(23):8868-75
references.pmid: 11102496
references.citation: J Neurosci. 1996 Apr 15;16(8):2495-507
references.pmid: 8786426
references.citation: Brain Res. 1975 Aug 8;93(2):363-6
references.pmid: 1174976
references.citation: J Endocrinol. 1975 Dec;67(3):461-2
references.pmid: 1206330
references.citation: Neuropharmacology. 1992 Jun;31(6):585-92
references.pmid: 1407398
references.citation: J Physiol. 1999 Jan 15;514 ( Pt 2):351-67
references.pmid: 9852319
references.citation: Neuroscience. 2001;106(3):621-31
references.pmid: 11591462
references.citation: Neuroscience. 1996 Aug;73(3):637-42
references.pmid: 8809784
references.citation: Neuroscience. 1981;6(4):619-43
references.pmid: 7017456
references.citation: Pflugers Arch. 2006 Aug;452(5):513-37
references.pmid: 16649055
references.citation: Brain Res. 1993 Apr 2;607(1-2):341-4
references.pmid: 8481811
references.citation: Mol Pharmacol. 2007 Dec;72(6):1447-56
references.pmid: 17785580
references.citation: J Physiol. 1995 Dec 15;489 ( Pt 3):805-11
references.pmid: 8788944
references.citation: Histochem Cell Biol. 2006 Oct;126(4):453-63
references.pmid: 16612582
references.citation: Prog Brain Res. 1983;60:115-22
references.pmid: 6665132
references.citation: Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3684-8
references.pmid: 8622997
references.citation: Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):8063-7
references.pmid: 8755603
references.citation: J Virol Methods. 1998 Jul;73(1):89-97
references.pmid: 9705180
references.citation: Brain Res. 1992 Oct 30;594(2):244-52
references.pmid: 1450950
references.citation: J Comp Neurol. 1999 Apr 28;407(1):11-32
references.pmid: 10213185
references.citation: Histochem Cell Biol. 2005 Nov;124(5):379-90
references.pmid: 16136347
references.citation: J Physiol. 1998 Aug 15;511 ( Pt 1):89-103
references.pmid: 9679166