Coronin 1B antagonizes cortactin and remodels Arp2/3-containing actin branches in lamellipodia.
The dendritic actin network generated by the Arp2/3 complex in lamellipodia underlies formation of protrusions, directional sensing, and migration. While the generation of this network is well studied, the mechanisms regulating network disassembly are poorly understood. We report that Coronin 1B disassembles Arp2/3-containing actin filament branches by inducing Arp2/3 dissociation. This activity is antagonized by Cortactin, a filament branch stabilizer. Consistent with this biochemical competition, depletion of both proteins partially rescues defects in lamellipodial dynamics observed upon depletion of either protein alone. Coronin 1B targets actin branches in a manner that is mutually exclusive with the Arp2/3 complex and alters the branch angle. We conclude that Coronin 1B replaces the Arp2/3 complex at actin filament branches as the dendritic network matures and drives the turnover of branched actin networks.
Cai
Liang
L
Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA.
Makhov
Alexander M
AM
Schafer
Dorothy A
DA
Bear
James E
JE
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D051377:Actin-Related Protein 2 / Q000378:metabolism*; D051378:Actin-Related Protein 3 / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D051356:Cortactin / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts; D006801:Humans; D051379:Mice; D011554:Pseudopodia; D051381:Rats; D054378:Acetogenins
D015107:4-Butyrolactone; D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins; D051356:Cortactin; C438920:coronin acetogenin; D054378:Acetogenins
Cell. 1999 May 14;97(4):435-47
10338208
Nat Rev Mol Cell Biol. 2006 Oct;7(10):713-26
16990851
Biophys J. 2005 Feb;88(2):1387-402
15556992
Annu Rev Biophys Biomol Struct. 2000;29:545-76
10940259
Curr Biol. 2005 Jul 26;15(14):1276-85
16051170
J Cell Biol. 1999 May 31;145(5):1009-26
10352018
Science. 2004 Sep 17;305(5691):1782-6
15375270
Annu Rev Cell Dev Biol. 2002;18:247-88
12142287
J Cell Sci. 2007 May 15;120(Pt 10):1779-90
17456547
Nat Cell Biol. 2006 Aug;8(8):826-33
16862144
Science. 2006 Aug 11;313(5788):839-42
16902139
Cell. 2007 Mar 9;128(5):915-29
17350576
Proc Natl Acad Sci U S A. 1998 May 26;95(11):6181-6
9600938
J Cell Biol. 2002 Dec 23;159(6):993-1004
12499356
J Biol Chem. 2005 Sep 9;280(36):31913-23
16027158
Physiology (Bethesda). 2006 Oct;21:352-61
16990456
Curr Biol. 2000 Oct 19;10(20):1273-82
11069108
Cell. 2003 Feb 21;112(4):453-65
12600310
Biophys J. 2006 Nov 1;91(9):3519-28
16905606
J Biol Chem. 2006 Oct 13;281(41):30947-56
16901905
Curr Biol. 2001 Mar 6;11(5):370-4
11267876
Trends Cell Biol. 2006 Aug;16(8):421-6
16806932
Nat Cell Biol. 2003 Sep;5(9):793-802
12942085
Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15009-13
11742068
J Cell Biol. 2000 Oct 2;151(1):29-40
11018051
PLoS Biol. 2005 Nov;3(11):e383
16262445
Curr Biol. 2007 Mar 6;17(5):395-406
17331727