Manipulation of major membrane lipid synthesis and its effects on sporulation in Saccharomyces cerevisiae.
During the sporulation process of Saccharomyces cerevisiae, meiotic progression is accompanied by de novo formation of the prospore membrane inside the cell. However, it remains to be determined whether certain species of lipids are required for spore formation in yeast. In this study, we analyzed the requirement of the synthesis of phosphatidylethanolamine (PE), phosphatidylcholine (PC), and ergosterol for spore formation using strains in which the synthesis of these lipids can be controlled. When synthesis of PE and PC was repressed, sporulation efficiency decreased. This suggests that synthesis of these phospholipids is vital to proper sporulation. In addition, sporulation was also impaired in cells with a lowered sterol content, raising the possibility that sterol content is also important for spore formation.
Deng
Lan
L
Department of Biotechnology, The University of Tokyo, Tokyo, Japan.
Nagasawa
Jumpei
J
Ono
Yusuke
Y
Ishikawa
Yasuhisa
Y
Kakihara
Toru
T
Fukuda
Ryouichi
R
Ohta
Akinori
A
D004875:Ergosterol / Q000096:biosynthesis*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008540:Meiosis; D008563:Membrane Lipids / Q000096:biosynthesis*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013172:Spores, Fungal / Q000235:genetics* / Q000378:metabolism
D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine; D004875:Ergosterol