Clenbuterol enhances the production of kynurenic acid in brain cortical slices and glial cultures.
The effect of a beta(2)-adrenergic agonist, clenbuterol on the production of a glutamate receptor antagonist, kynurenic acid was studied in vitro. Clenbuterol enhanced the production of kynurenic acid in brain cortical slices (0.1-1.0 mM) and in glial cultures (1-50 muM). Timolol, a non-selective beta-adrenergic antagonist prevented this effect. The presented data indicate a novel mechanism of action of beta(2)-adrenoceptor agonists and suggest that an increased formation of the endogenous glutamate receptor antagonist, kynurenic acid could partially contribute to their neuroprotective activity.
Luchowska
Elzbieta
E
Department of Pharmacology and Clinical Pharmacology, Skubiszewski Medical School, Jaczewskiego 8, PL 20-090 Lublin, Poland.
Kloc
Renata
R
Wnuk
Sebastian
S
Olajossy
Bartosz
B
Wielosz
Marian
M
Urbańska
Ewa M
EM
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism*; D002976:Clenbuterol / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000378:metabolism*; D066298:In Vitro Techniques; D007736:Kynurenic Acid / Q000378:metabolism*; D008297:Male; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000637:Transaminases / Q000378:metabolism
D000318:Adrenergic beta-Agonists; D018691:Excitatory Amino Acid Antagonists; D000637:Transaminases; C021481:kynurenine-oxoglutarate transaminase; D007736:Kynurenic Acid; D002976:Clenbuterol