title: Propofol and N-Acetylcysteine attenuate oxidative stress induced by intestinal ischemia/reperfusion in rats: protein carbonyl detection by immunoblotting.
abstract: OBJECTIVE
  To evaluate the antioxidant effect of Propofol and N-Acetylcysteine (NAC) on intestinal ischemia/reperfusion (I/R) in rats by determining carbonyl protein level.
  
  
  METHODS
  Forty Wistar rats were randomly assigned into the following groups: Control; Sham; I/R with Propofol; I/R with Propofol and NAC; I/R with Ketamine and Xylazine. The I/R groups underwent 60 minutes of ischemia and an equal period of reperfusion. Blood samples, collected by cardiac punction, were centrifuged for plasma obtainment. Protein carbonyl level in plasma samples was determined by immunoblotting.
  
  
  RESULTS
  No significant difference in protein carbonyl level was found between Control and Sham groups (P>0.05). The highest reduction in protein carbonyl level (P<0.05) was obtained with the administration of Propofol and NAC (Group 4) in intestinal I/R procedure.
  
  
  CONCLUSIONS
  The administration of Propofol and NAC showed the best antioxidant effect on oxidative stress in rats that underwent intestinal I/R procedure, suggesting a synergistic interaction.
authors.affiliation: Faculty of Medicine, UFRJ, Rio de Janeiro, Brazil.
authors.forename: Marcelo Aragão Insuellas de
authors.initials: MA
authors.lastname: Azeredo
authors.forename: Luciana Aragão Insuellas de
authors.initials: LA
authors.lastname: Azeredo
authors.forename: Elis Cristina Araújo
authors.initials: EC
authors.lastname: Eleuthério
authors.forename: Alberto
authors.initials: A
authors.lastname: Schanaider
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007422:Intestines / Q000098:blood supply*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D015742:Propofol / Q000494:pharmacology*; D050050:Protein Carbonylation / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D016166:Free Radical Scavengers; D000111:Acetylcysteine; D015742:Propofol