title: The intra-nucleus integration of mitochondrial DNA (mtDNA)in cervical mucosa cells and its relation with c-myc expression.
abstract: OBJECTIVE
  To explore the relationship between the integration of mitochondrial DNA(mtDNA) in the nuclei of cervical epithelium cells and the expression of c-myc.
  
  
  METHODS
  The expression of c-myc protein was measured by immunohistochemical test in 40 cases of the uterine cervix cancer, 30 cases of cervical intraepithelial neoplasia (CIN) and 30 cases of normal cervical epithelium; the sequence of mtDNA in the nuclei was detected by in situ hybridization technique.
  
  
  RESULTS
  The detection rates of mtDNA in the nuclei of cervical epithelium cells were 27.5%, 13.3% and 0% in cervical carcinoma, CIN, and normal cervical epithelium respectively. The expression rate of c-myc in cervical mucoma cells was 67% in the mtDNA sequence positive group and was significantly higher than that in the negative group (36%).
  
  
  CONCLUSIONS
  The integration of mtDNA into the nuclei of cervical epithelium cells may be involved in the carcinogenesis of cervical epithelium cells and the expression of c-myc might be related to the integration of mtDNA sequence into nuclei of cervical epithelium cells.
authors.affiliation: Center Laboratory, Wuxi Hospital for Matemaland Child Health Care, Affiliated Medical University of Nanjing, Wuxi, PR China. chendaozhen@163.com
authors.forename: Daozhen
authors.initials: D
authors.lastname: Chen
authors.forename: Wenqun
authors.initials: W
authors.lastname: Xue
authors.forename: Jinying
authors.initials: J
authors.lastname: Xiang
mesh_terms: D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology
chemical_list: D004272:DNA, Mitochondrial; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc
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