Induction of apoptosis by KI0477959 through activation of caspase-3 in human leukemia cell line, HL-60 cells.
KI0477959 (Herbkines) has been used for the purpose of development of physical strength in wasting diseases, like cancer. In the present study, apoptosis-inducing activities of butanol fraction of KI0477959 were studied in human leukemia cell line, HL-60 cells. KI0477959 increased cytotoxicity but had less effect on human peripheral blood mononuclear cells. KI0477959-induced apoptosis was accompanied by activation of caspase-3 and specific proteolytic cleavage of poly-ADP-ribose polymerase. Increased apoptosis was reduced by treatment with p38 and extracellular signal-regulated protein kinase (ERK) inhibitors. These results suggest that KI0477959 induces apoptosis through activation of caspase-3, p38, and ERK in HL-60 cells.
Jeong
Hyun-Ja
HJ
Biochip Research Center, Hoseo University, Chungnam, South Korea.
Lee
Ju-Young
JY
Kim
Joon-Bae
JB
Go
Hoyeon
H
Ko
Seong-Gyu
SG
Seo
Young-Wan
YW
Jeong
Sejin
S
Park
Jinhan
J
Na
Ho-Jeong
HJ
Um
Jae-Young
JY
Kim
Hyung-Min
HM
Hong
Seung-Heon
SH
D000704:Analysis of Variance; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024161:Collagen Type XI / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology; D055670:Medicine, Korean Traditional; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors
C499319:COL11A2 protein, human; D024161:Collagen Type XI; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C488392:Herbkines; D010936:Plant Extracts; D020929:Mitogen-Activated Protein Kinase Kinases; D053148:Caspase 3