title: In vivo bioluminescence imaging in an experimental mouse model for dendritic cell based immunotherapy against malignant glioma.
abstract: The value of bioluminescence imaging (BLI) for experimental cancer models has become firmly established. We applied BLI to the GL261 glioma model in the context of dendritic cell (DC) immunotherapy. Initial validation revealed robust linear correlations between in vivo, ex vivo and in vitro luciferase activity measurements. Ex vivo BLI demonstrated midline crossing and leakage of tumor cells. Orthotopically challenged mice followed with BLI showed an initial adaptation phase, after which imaging data correlated linearly with stereologically determined tumor dimensions. Transition from healthy to moribund state corresponded with an increasing in vivo flux but the onset of neurological deficit was clearly delayed compared to the onset of in vivo flux increase. BLI was implemented in prophylactic immunotherapy and imaging data were prognostic for therapy outcome. Three distinct response patterns were detected. Our data underscore the feasibility of in vivo BLI in an experimental immunotherapeutic setting in the GL261 glioma model.
authors.affiliation: Department of Experimental Medicine, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: W
authors.initials: W
authors.lastname: Maes
authors.forename: C
authors.initials: C
authors.lastname: Deroose
authors.forename: V
authors.initials: V
authors.lastname: Reumers
authors.forename: O
authors.initials: O
authors.lastname: Krylyshkina
authors.forename: R
authors.initials: R
authors.lastname: Gijsbers
authors.forename: V
authors.initials: V
authors.lastname: Baekelandt
authors.forename: J
authors.initials: J
authors.lastname: Ceuppens
authors.forename: Z
authors.initials: Z
authors.lastname: Debyser
authors.forename: S W
authors.initials: SW
authors.lastname: Van Gool
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000628:therapy*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology*; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005910:Glioma / Q000175:diagnosis* / Q000628:therapy*; D007167:Immunotherapy / Q000379:methods*; D016014:Linear Models; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation / Q000379:methods; D015203:Reproducibility of Results; D016019:Survival Analysis; D013997:Time Factors; D014161:Transduction, Genetic / Q000379:methods
chemical_list: D000951:Antigens, Neoplasm; D008156:Luciferases
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