title: Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana.
abstract: BACKGROUND
  Pathogenesis-related proteins belonging to group 10 (PR10) are elevated in response to biotic and abiotic stresses in plants. Previously, we have shown a drastic salinity-induced increase in the levels of ABR17, a member of the PR10 family, in pea. Furthermore, we have also demonstrated that the constitutive expression of pea ABR17 cDNA in Arabidopsis thaliana and Brassica napus enhances their germination and early seedling growth under stress. Although it has been reported that several members of the PR10 family including ABR17 possess RNase activity, the exact mechanism by which the aforementioned characteristics are conferred by ABR17 is unknown at this time. We hypothesized that a study of differences in transcriptome between wild type (WT) and ABR17 transgenic A. thaliana may shed light on this process.
  
  
  RESULTS
  The molecular changes brought about by the expression of pea ABR17 cDNA in A. thaliana in the presence or absence of salt stress were investigated using microarrays consisting of 70-mer oligonucleotide probes representing 23,686 Arabidopsis genes. Statistical analysis identified number of genes which were over represented among up- or down-regulated transcripts in the transgenic line. Our results highlight the important roles of many abscisic acid (ABA) and cytokinin (CK) responsive genes in ABR17 transgenic lines. Although the transcriptional changes followed a general salt response theme in both WT and transgenic seedlings under salt stress, many genes exhibited differential expression patterns when the transgenic and WT lines were compared. These genes include plant defensins, heat shock proteins, other defense related genes, and several transcriptional factors. Our microarray results for selected genes were validated using quantitative real-time PCR.
  
  
  CONCLUSIONS
  Transcriptional analysis in ABR17 transgenic Arabidopsis plants, both under normal and saline conditions, revealed significant changes in abundance of transcripts for many stress responsive genes, as well as those related to plant growth and development. Our results also suggest that ABR17 may mediate stress tolerance through the modulation of many ABA- and CK-responsive genes and may further our understanding of the role of ABR17 in mediating plant stress responses.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada. krishnas@ualberta.ca
authors.forename: Sowmya S
authors.initials: SS
authors.lastname: Krishnaswamy
authors.forename: Sanjeeva
authors.initials: S
authors.lastname: Srivastava
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mohammadi
authors.forename: Muhammad H
authors.initials: MH
authors.lastname: Rahman
authors.forename: Michael K
authors.initials: MK
authors.lastname: Deyholos
authors.forename: Nat N V
authors.initials: NN
authors.lastname: Kav
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D003583:Cytokinins / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D018532:Pisum sativum / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: C086000:ABR17 protein, Pisum sativum; D003583:Cytokinins; D010940:Plant Proteins; D018749:RNA, Plant; D012965:Sodium Chloride; D000040:Abscisic Acid
references.citation: Biochem Biophys Res Commun. 2002 Oct 18;298(1):46-53
references.pmid: 12379218
references.citation: Plant Mol Biol. 2004 Jul;55(4):607-18
references.pmid: 15604704
references.citation: Plant J. 1998 Nov;16(4):433-42
references.pmid: 9881163
references.citation: Plant J. 2006 Mar;45(6):1028-36
references.pmid: 16507092
references.citation: J Mol Biol. 2002 Jun 21;319(5):1223-34
references.pmid: 12079359
references.citation: Plant Physiol. 2003 Apr;131(4):1748-55
references.pmid: 12692333
references.citation: Plant Mol Biol. 2001 Jul;46(4):491-504
references.pmid: 11485205
references.citation: Plant Mol Biol. 1988 Sep;11(5):713-5
references.pmid: 24272504
references.citation: Plant Physiol. 1994 Feb;104(2):339-347
references.pmid: 12232085
references.citation: Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):99-107
references.pmid: 15608381
references.citation: Plant Physiol. 1958 May;33(3):190-4
references.pmid: 16655111
references.citation: Biochem Biophys Res Commun. 1965 Dec 21;21(6):523-30
references.pmid: 5879460
references.citation: Plant Mol Biol. 1996 Jul;31(4):863-76
references.pmid: 8806416
references.citation: Plant Cell. 1990 Jun;2(6):503-12
references.pmid: 2152172
references.citation: Plant Cell Physiol. 2004 Sep;45(9):1320-4
references.pmid: 15509856
references.citation: Planta. 1994;193(3):470-2
references.pmid: 7764876
references.citation: Plant Physiol. 1958 Mar;33(2):115-7
references.pmid: 16655089
references.citation: J Exp Bot. 2006;57(5):1097-107
references.pmid: 16510518
references.citation: Plant Mol Biol. 1992 Feb;18(4):749-57
references.pmid: 1558948
references.citation: Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11812-7
references.pmid: 11562463
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):12987-92
references.pmid: 16924117
references.citation: Biochim Biophys Acta. 2000 Jun 21;1492(1):1-14
references.pmid: 10858526
references.citation: Plant Physiol. 2006 Sep;142(1):113-23
references.pmid: 16844836
references.citation: Plant J. 2002 Aug;31(3):279-92
references.pmid: 12164808
references.citation: Plant Physiol. 2000 Mar;122(3):705-14
references.pmid: 10712533
references.citation: Plant Mol Biol. 2003 Oct;53(3):383-97
references.pmid: 14750526
references.citation: Biochem J. 2003 Apr 1;371(Pt 1):97-108
references.pmid: 12646039
references.citation: BMC Plant Biol. 2006 Oct 12;6:25
references.pmid: 17038189
references.citation: Proc Natl Acad Sci U S A. 1985 Jul;82(13):4399-403
references.pmid: 16593579
references.citation: FEBS Lett. 2003 Nov 20;554(3):373-80
references.pmid: 14623097
references.citation: Curr Opin Plant Biol. 2001 Oct;4(5):392-400
references.pmid: 11597496
references.citation: Plant J. 2001 Sep;27(5):407-15
references.pmid: 11576425
references.citation: Genome Biol. 2007;8(4):R49
references.pmid: 17408486
references.citation: EMBO J. 1989 Jul;8(7):1935-8
references.pmid: 2571499
references.citation: FEBS Lett. 2001 Jun 8;498(2-3):187-9
references.pmid: 11412854
references.citation: Plant Cell Physiol. 2000 Jul;41(7):898-903
references.pmid: 10965948
references.citation: Cell. 1989 Sep 8;58(5):991-9
references.pmid: 2776216
references.citation: Trends Plant Sci. 2005 Feb;10(2):79-87
references.pmid: 15708345
references.citation: Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9783-8
references.pmid: 10931949
references.citation: Biotechniques. 2003 Feb;34(2):374-8
references.pmid: 12613259
references.citation: Plant Biotechnol J. 2006 Sep;4(5):529-49
references.pmid: 17309728
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:281-309
references.pmid: 15012236
references.citation: Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):10098-103
references.pmid: 16785434
references.citation: Plant J. 2005 Dec;44(6):903-16
references.pmid: 16359384
references.citation: Plant Physiol. 2004 Aug;135(4):2055-67
references.pmid: 15299136
references.citation: Plant Physiol. 2004 Jul;135(3):1697-709
references.pmid: 15247402
references.citation: Plant Cell. 1994 Mar;6(3):351-60
references.pmid: 8180497
references.citation: Eur J Biochem. 1998 Dec 1;258(2):794-802
references.pmid: 9874249
references.citation: Plant Cell. 1998 Sep;10(9):1427-37
references.pmid: 9724690
references.citation: Plant Cell. 2004 Sep;16(9):2481-98
references.pmid: 15319476
references.citation: Tree Physiol. 2001 Jul;21(10):633-43
references.pmid: 11446992
references.citation: Bioinformatics. 2005 May 1;21(9):1995-2000
references.pmid: 15691855
references.citation: Plant Mol Biol. 1985 Mar;4(2-3):81-6
references.pmid: 24310743
references.citation: Plant Mol Biol. 1986 Nov;7(6):441-9
references.pmid: 24302472
references.citation: Plant Physiol. 2001 Nov;127(3):842-51
references.pmid: 11706167
references.citation: J Biol Chem. 2002 Jun 28;277(26):23684-92
references.pmid: 11953433
references.citation: Plant Physiol. 1956 Jul;31(4):318-9
references.pmid: 16654890
references.citation: Plant Physiol. 1999 Aug;120(4):1043-8
references.pmid: 10444087
references.citation: Plant J. 2000 Oct;24(1):103-11
references.pmid: 11029708
references.citation: Plant J. 2003 Feb;33(4):677-90
references.pmid: 12609041
references.citation: Plant Physiol. 2003 Aug;132(4):1998-2011
references.pmid: 12913156
references.citation: Plant J. 2004 Sep;39(6):863-76
references.pmid: 15341629
references.citation: Plant J. 2005 Oct;44(2):314-33
references.pmid: 16212609
references.citation: Plant Mol Biol. 1991 Apr;16(4):547-65
references.pmid: 1714316
references.citation: Plant Cell. 2002 Dec;14(12):3237-53
references.pmid: 12468740
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Plant Cell. 1995 Feb;7(2):173-82
references.pmid: 7756828
references.citation: Plant J. 2004 Apr;38(1):142-51
references.pmid: 15053767
references.citation: Genes Dev. 2000 Dec 1;14(23):3024-36
references.pmid: 11114891
references.citation: Plant Mol Biol. 1994 Jan;24(1):235-40
references.pmid: 8111022
references.citation: Planta. 1967 Jun;78(2):200-7
references.pmid: 24522710
references.citation: Cell. 1996 Apr 19;85(2):159-70
references.pmid: 8612269
references.citation: Science. 2000 Dec 15;290(5499):2105-10
references.pmid: 11118137
references.citation: Cell. 1998 Jan 9;92(1):93-103
references.pmid: 9489703
references.citation: Plant Physiol. 1987 Oct;85(2):529-36
references.pmid: 16665731
references.citation: Development. 2005 Apr;132(7):1555-65
references.pmid: 15728668
references.citation: J Exp Bot. 2005 Feb;56(412):673-83
references.pmid: 15642717
references.citation: Plant Cell. 1997 Jun;9(6):841-57
references.pmid: 9212461
references.citation: Plant Mol Biol. 1995 Jun;28(3):455-71
references.pmid: 7632916
references.citation: Plant Physiol. 1994 Mar;104(3):1015-25
references.pmid: 7513083
references.citation: Plant Cell Physiol. 2003 Dec;44(12):1412-6
references.pmid: 14701937
references.citation: J Mol Biol. 2003 Jan 3;325(1):123-33
references.pmid: 12473456
references.citation: Plant Physiol. 1995 Jan;107(1):87-100
references.pmid: 11536663