title: Rapid recycling of beta-adrenergic receptors is dependent on the actin cytoskeleton and myosin Vb.
abstract: For the beta(2)-adrenergic receptor (beta(2)AR), published evidence suggests that an intact actin cytoskeleton is required for the endocytosis of receptors and their proper sorting to the rapid recycling pathway. We have characterized the role of the actin cytoskeleton in the regulation of beta(2)AR trafficking in human embryonic kidney 293 (HEK293) cells using two distinct actin filament disrupting compounds, cytochalasin D and latrunculin B (LB). In cells pretreated with either drug, beta(2)AR internalization into transferrin-positive vesicles was not altered but both agents significantly decreased the rate at which beta(2)ARs recycled to the cell surface. In LB-treated cells, nonrecycled beta(2)ARs were localized to early embryonic antigen 1-positive endosomes and also accumulated in the recycling endosome (RE), but only a small fraction of receptors localized to LAMP-positive late endosomes and lysosomes. Treatment with LB also markedly enhanced the inhibitory effect of rab11 overexpression on receptor recycling. Dissociating receptors from actin by expression of the myosin Vb tail fragment resulted in missorting of beta(2)ARs to the RE, while the expression of various CART fragments or the depletion of actinin-4 had no detectable effect on beta(2)AR sorting. These results indicate that the actin cytoskeleton is required for the efficient recycling of beta(2)ARs, a process that likely is dependent on myosin Vb.
authors.affiliation: Department of Pediatrics, Baylor College of Medicine, 6621 Fannin, CCC 1040.00, Houston, TX 77030, USA.
authors.forename: Ellen E
authors.initials: EE
authors.lastname: Millman
authors.forename: Haibin
authors.initials: H
authors.lastname: Zhang
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
authors.forename: Veronica
authors.initials: V
authors.lastname: Godines
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Bean
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Knoll
authors.forename: Robert H
authors.initials: RH
authors.lastname: Moore
mesh_terms: D000199:Actins / Q000378:metabolism*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002460:Cell Line; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism*; D004705:Endocytosis / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024701:Myosin Type V / Q000378:metabolism*; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism*; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; C505832:MYO5B protein, human; D018343:Receptors, Adrenergic, beta-2; D053778:Thiazolidines; D015638:Cytochalasin D; D024701:Myosin Type V; D018995:Myosin Heavy Chains; C037068:latrunculin B
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