Thy-1 is expressed in myofibroblasts but not found in hepatic stellate cells following liver injury.
Thy-1 (CD90) is an adhesion molecule induced in fibroblast populations associated with wound healing and fibrosis. In this study the question whether Thy-1-gene-expression can be induced in hepatic stellate cells (HSC) in vivo, under conditions of liver injury or liver regeneration was addressed. Acute and chronic rat liver injury was induced by the administration of CCl4. For comparison, cirrhotic human liver, and rat 67% partial hepatectomy (PH) was studied as well. Thy-1-gene-expression was examined also in isolated human liver myofibroblasts. Thy-1-mRNA expression was significantly upregulated in chronic liver injury. Thy-1+ cells were detected in the periportal area of rat liver specimens in normal-, injured- and regenerative-conditions. In chronic human and rat liver injury, Thy-1+ cells were located predominantly in scar tissue. In the pericentral necrotic zone after CCl4-treatment, no induction of Thy-1 was found. Gremlin and Thy-1 showed comparable localization in the periportal areas. Thy-1 was not detected in either normal or capillarized sinusoids, in isolated rat HSC, and was neither inducible by inflammatory cytokines in isolated HSC, nor upregulated in treated myofibroblasts. Based upon these data Thy-1 is not a marker of "activated" sinusoidal HSC, but it is a marker of "activated" (myo)fibroblasts found in portal areas and in scar tissue.
Dudas
Jozsef
J
Department of Internal Medicine, Section of Gastroenterology and Endocrinology, Georg-August-University Göttingen, Robert-Koch-Strasse 40, 37075 Göttingen, Germany.
Mansuroglu
Tümen
T
Batusic
Danko
D
Ramadori
Giuliano
G
D000818:Animals; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism* / Q000473:pathology*; D008115:Liver Regeneration; D051381:Rats; D017208:Rats, Wistar; D018800:Thy-1 Antigens / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D018800:Thy-1 Antigens
Am J Pathol. 1982 Feb;106(2):145-55
7065113
Differentiation. 2008 Sep;76(7):784-94
18177423
Hepatology. 2001 Jan;33(1):177-88
11124834
J Cell Biol. 1994 Dec;127(6 Pt 2):2037-48
7806580
Am J Pathol. 1997 Nov;151(5):1265-72
9358752
Cell Tissue Res. 2003 Sep;313(3):301-11
12898209
J Hepatol. 1996 Jun;24(6):719-30
8835748
Circ Res. 1998 May 4;82(8):845-51
9576104
Exp Cell Res. 2004 May 1;295(2):488-96
15093746
J Hepatol. 2006 Sep;45(3):429-38
16846660
Histochem Cell Biol. 1999 Nov;112(5):387-401
10603079
Int J Exp Pathol. 1992 Dec;73(6):765-72
1493105
Am J Pathol. 2007 Nov;171(5):1608-18
17916596
J Mol Cell Cardiol. 2007 May;42(5):991-1000
17395197
Cancer Res. 2001 Nov 15;61(22):8079-84
11719431
Am J Physiol Gastrointest Liver Physiol. 2002 Aug;283(2):G435-44
12121892
Lab Invest. 2004 Feb;84(2):153-9
14688800
Lab Invest. 1998 Feb;78(2):185-94
9484716
J Hepatol. 1995 Sep;23(3):295-306
8550994
J Cell Physiol. 2004 Jun;199(3):388-98
15095286
Exp Eye Res. 2004 Nov;79(5):705-12
15500828
Hepatology. 1996 Aug;24(2):352-60
8690404
Histochem Cell Biol. 2006 Nov;126(5):549-62
16770575
Virchows Arch B Cell Pathol Incl Mol Pathol. 1990;59(6):349-57
1705733
Am J Physiol Gastrointest Liver Physiol. 2006 Sep;291(3):G482-90
16574981
J Immunol. 1996 Jul 15;157(2):874-83
8752941
Hepatology. 1992 Jan;15(1):5-11
1530788
Cell Tissue Res. 2007 Sep;329(3):503-14
17576600
J Hepatol. 2002 Oct;37(4):527-35
12217608
Liver. 2002 Aug;22(4):283-94
12296961
Histochem Cell Biol. 2007 Feb;127(2):161-74
17024455
Hepatology. 1996 Jan;23(1):193
8550043
Hepatol Res. 2005 May;32(1):1-8
15866513
J Biomed Sci. 2003 May-Jun;10(3):352-8
12711863
Blood. 2008 Feb 15;111(4):2427-35
18042797
Cancer Genet Cytogenet. 2005 Sep;161(2):151-8
16102586
Biochemistry. 1979 Nov 27;18(24):5294-9
518835
Int J Biochem Cell Biol. 2006 Feb;38(2):135-51
16257564
Eur J Cell Biol. 2004 Sep;83(9):469-76
15540463
J Exp Med. 1964 Sep 1;120:413-33
14207060
Gastroenterology. 1999 Nov;117(5):1205-21
10535885
Exp Cell Res. 2006 Nov 15;312(19):3752-67
17027000
Am J Pathol. 2003 Oct;163(4):1291-300
14507638
Eur J Gastroenterol Hepatol. 2005 May;17(5):547-57
15827446