Blood serum levels of vascular cell adhesion molecule (sVCAM-1), intercellular adhesion molecule (sICAM-1) and endothelial leucocyte adhesion molecule-1 (ELAM-1) in diabetic retinopathy.
BACKGROUND
Interaction between cells via intimate cell-cell contact is facilitated by a cell surface molecules, termed adhesion molecules. The aim of the study was to evaluate the blood serum concentration of soluble forms of vascular cell adhesion molecule (VCAM-1), intercellular adhesion molecule (ICAM-1) and endothelial leukocyte adhesion molecule-1 (ELAM-1) in patients with type 1 diabetes mellitus without and with diabetic retinopathy.


METHODS
The study was performed in 75 patients with type 1 diabetes mellitus, 35 without retinopathy (group 1) and 40 with retinopathy (group 2). Soluble forms of VCAM-1, ICAM-1 and ELAM-1 were determined by enzyme-linked immunosorbent assay (ELISA).


RESULTS
The serum concentration of sICAM-1 and sELAM-1 were significantly elevated and the concentration sVCAM-1 was elevated but not significantly in diabetic patients when compared with control subjects. There was a significant difference in VCAM-1 concentrations between the control group and group 2 (965.9 +/- 229.0 vs. 1283.7 +/- 387.6 ng/ml, p < 0.05) and between group 1 and group 2 (1115.0 +/- 285.5 vs. 1283.7 +/- 387.6 ng/ml, p < 0.05). There were significant differences in sICAM-1 concentrations between the control group and group 1 (p < 0.05) and between the control group and group 2 (p < 0.05). Where was no significant difference in sICAM-1 concentration between group 1 and 2 (405.2 +/- 135.9 vs. 443.1 +/- 112.7 ng/ml, p = 0.08). ELAM-1 concentration was significantly elevated in group 2 (120.5 +/- 49.3 ng/ml) when compared with the control group (51.7 +/- 18.1 ng/ml, p < 0.005) and with group 1 (81.2 +/- 27.7 ng/ml, p < 0.05).


CONCLUSIONS
The correlations found between sVCAM-1, sICAM-1 and sELAM-1 and the presence of retinopathy suggest that cellular adhesion and neovascularization may be linked processes.
Nowak
Mariusz
M
Pathophysiology Division, Department of Pathophysiology and Endocrinology, Medical University of Silesia, 41-800 Zabrze pl., Traugutta 2, Poland. nowak-mar@wp.pl
Wielkoszyński
Tomasz
T
Marek
Bogdan
B
Kos-Kudła
Beata
B
Swietochowska
Elzbieta
E
Siemińska
Lucyna
L
Kajdaniuk
Dariusz
D
Głogowska-Szelag
Joanna
J
Nowak
Katarzyna
K
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000150:complications; D003930:Diabetic Retinopathy / Q000097:blood*; D019040:E-Selectin / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood*
D019040:E-Selectin; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1
N Engl J Med. 1993 Sep 30;329(14):977-86
8366922
Diabetologia. 1997 Oct;40(10):1166-71
9349597
Diabetologia. 1990 Sep;33(9):538-41
2253830
Coron Artery Dis. 1995 Jun;6(6):497-502
7551271
Acta Diabetol. 2004 Sep;41(3):118-22
15666579
J Clin Invest. 1998 May 1;101(9):1905-15
9576755
Ann N Y Acad Sci. 1992 Dec 4;667:324-31
1285023
Invest Ophthalmol Vis Sci. 1994 Nov;35(12):3974-9
7525506
Endocrine. 2000 Feb;12(1):21-3
10855686
Int J Mol Med. 2005 Mar;15(3):459-62
15702238
Diabet Med. 2000 Sep;17(9):644-9
11051283
Int J Cardiol. 2004 Aug;96(2):235-40
15262039
Diabetes Res Clin Pract. 1999 Nov;46(2):143-8
10724093
Eur J Intern Med. 2002 May;13(3):185-189
12020626
Curr Eye Res. 1999 Sep;19(3):219-27
10487959
Immunol Today. 1993 Oct;14(10):506-12
7506035
Graefes Arch Clin Exp Ophthalmol. 1994 May;232(5):302-7
8045440
Nature. 1995 Aug 10;376(6540):517-9
7543654
Int J Cardiol. 2006 Apr 4;108(2):224-30
16002164
Int Angiol. 2004 Jun;23(2):128-33
15507889
J Clin Endocrinol Metab. 1996 Dec;81(12):4313-7
8954033
Eur Heart J. 1999 Jul;20(14):1039-43
10381855
Diabetes. 1994 Feb;43(2):169-72
8288039
Diabet Med. 2002 Oct;19(10):822-6
12358868