The first examples of mRNA cap analogs bearing boranophosphate modification.
The syntheses of mRNA cap analogs modified with boranophosphate moiety at either the alpha or beta-position of the 5', 5'-triphosphate bridge (m(7)Gppp(BH3)G, m(7)Gpp(BH3)pG and m(7)Gpp(BH3)pm(7)G) are described. The preliminary biological characterization of these compounds revealed that they have high affinity for translational factor eIF4E and high potency to inhibit cap-dependent translation in cell free system. The analogs modified at the beta-position were also found to be resistant to DcpS decapping pyrophosphatase.
Kowalska
Joanna
J
Division of Biophysics, Institute of Experimental Physics, University of Warsaw, 02-089 Warsaw, Poland.
Zuberek
Joanna
J
Darzynkiewicz
Zbigniew M
ZM
Lukaszewicz
Maciej
M
Darzynkiewicz
Edward
E
Jemielity
Jacek
J
D001880:Boranes / Q000737:chemistry*; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry*; D014176:Protein Biosynthesis; D012314:RNA Cap Analogs / Q000138:chemical synthesis / Q000737:chemistry*
D001880:Boranes; D039561:Eukaryotic Initiation Factor-4E; D010710:Phosphates; D012314:RNA Cap Analogs; C510710:boranophosphate