Temperature-gradient dependent detection of target DNA oligomers using DNA-immobilized open tubular capillary column.
A DNA-immobilized open tubular capillary column (DNA-immobilized OTC) system was developed for analysis and separation of target DNA oligomers. By combining the DNA-immobilized OTC column with a nano/micro-flow pump and a high-sensitivity UV detector, we succeeded in small-scale selective detection of target DNA oligomers (> 0.02 pmol). We designed a temperature-gradient strategy for the efficient separation of target DNA.
Kamisetty
Nagendra Kumar
NK
Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
Pack
Seung Pil
SP
Devarayapalli
Kamakshaiah Charyulu
KC
Nonogawa
Mitsuru
M
Kodaki
Tsutomo
T
Makino
Keisuke
K
D002846:Chromatography, Affinity / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides