Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development.
The gene GhCPK1 encoding a calcium dependent protein kinase was identified from cotton. Transcripts of GhCPK1 accumulated primarily in the elongating fiber, and Arabidopsis plants transformed with GhCPK1 promoter-GUS construct exhibited GUS activity mainly in the developing trichomes, roots, young leaves and sepals. In the bombarded onion epidermal cells, GhCPK1-GFP fusion proteins showed a subcellular distribution in the plasma membrane. In vitro assays indicated that GhCPK1 was a functional calcium-dependent kinase able to undergo autophosphorylation and phosphorylation of the known substrate histone III-S. Together, these results suggest that GhCPK1 may play a role in the calcium signaling events associated with fiber elongation.
Huang
Quan-Sheng
QS
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094, China.
Wang
Hai-Yun
HY
Gao
Peng
P
Wang
Guo-Ying
GY
Xia
Gui-Xian
GX
D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D020013:Calcium Signaling; D003001:Cloning, Molecular; D032561:Cotton Fiber; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D017931:DNA Primers; D018744:DNA, Plant; D011494:Protein Kinases; D011994:Recombinant Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases
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