Highly sensitive spectrofluorimetric determination of trace amounts of prulifloxacin using the aluminium(III)-prulifloxacin system.
The fluorescence of the prulifloxacin (PUFX)-Al(III) system was investigated . Experiments indicated that the fluorescence intensity of prulifloxacin could be greatly enhanced by Al(III) and sensitized by sodium dodecylbenzene sulphonate (SDBS). Accordingly, a sensitive spectrofluorimetric method for the determination of prulifloxacin was established. While excited at 275 nm, the enhanced fluorescence intensity at 412 nm of the system (DeltaF) showed a good linear relationship with the concentration of prulifloxacin within the range 4.0 x 10(-8)-3.0 x 10(-6) mol/L. The regression equation was DeltaF = 9.83 + 10.8 x 10(7)c (mol/L); the correlation coefficient and detection limit (3sigma/k) were 0.99901 and 2.0 x 10(-8) mol/L, respectively. The proposed method has been successfully applied to determine prulifloxacin in real pharmaceutical samples. The luminescence mechanism of the system is also discussed in detail.
Yu
Fengshan
F
Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, College of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.
Liu
Weizhou
W
Chen
Fang
F
Cai
Ping
P
D000535:Aluminum / Q000737:chemistry*; D001557:Benzenesulfonates / Q000737:chemistry*; D002412:Cations; D004148:Dioxolanes / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D024841:Fluoroquinolones / Q000032:analysis* / Q000737:chemistry; D010879:Piperazines / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D001557:Benzenesulfonates; D002412:Cations; D004148:Dioxolanes; D005456:Fluorescent Dyes; D024841:Fluoroquinolones; D010879:Piperazines; C001114:dodecylbenzenesulfonic acid; D000535:Aluminum; C074191:prulifloxacin