title: Effect of astaxanthin in combination with alpha-tocopherol or ascorbic acid against oxidative damage in diabetic ODS rats.
abstract: The present study was performed to investigate the effect of astaxanthin in combination with other antioxidants against oxidative damage in streptozotocin (STZ)-induced diabetic Osteogenic Disorder Shionogi (ODS) rats. Diabetic-ODS rats were divided into five groups: control, astaxanthin, ascorbic acid, alpha-tocopherol, and tocotrienol. Each of the four experimental groups was administered a diet containing astaxanthin (0.1 g/kg), in combination with ascorbic acid (3.0 g/kg), alpha-tocopherol (0.1 g/kg), or tocotrienol (0.1 g/kg) for 20 wk. The effects of astaxanthin with other antioxidants on lipid peroxidation, urinary 8-hydroxy-2-deoxyguanosine (8-OHdG) excretion, serum creatinine (Cr) level, creatinine clearance (Ccr), and urinary protein content were assessed. The serum lipid peroxide levels and chemiluminescent (CL) intensity in the liver of the alpha-tocopherol and tocotrienol groups were significantly reduced in comparison to that of the control group. In the alpha-tocopherol group, urinary 8-OHdG excretion, serum Cr level, Ccr, urinary albumin excretion, and urinary protein concentration were significantly decreased as compared with those in the control group. Additionally, the CL intensity in the kidney of the alpha-tocopherol group was significantly lower, but that of the ascorbic acid group was significantly higher than that in the control group. These results indicate that dietary astaxanthin in combination with alpha-tocopherol has an inhibitory effect on oxidative stress. On the other hand, our study suggests that excessive ascorbic acid intake increases lipid peroxidation in diabetic rats.
authors.affiliation: Department of Food and Nutrition, Japan Women's University Japan Women's University Tokyo 112-8681, Japan. masako.nakano06@gr.jwu.ac.jp
authors.forename: Masako
authors.initials: M
authors.lastname: Nakano
authors.forename: Aya
authors.initials: A
authors.lastname: Onodera
authors.forename: Emi
authors.initials: E
authors.lastname: Saito
authors.forename: Miyako
authors.initials: M
authors.lastname: Tanabe
authors.forename: Kazue
authors.initials: K
authors.lastname: Yajima
authors.forename: Jiro
authors.initials: J
authors.lastname: Takahashi
authors.forename: Van Chuyen
authors.initials: VC
authors.lastname: Nguyen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000378:metabolism; D003929:Diabetic Neuropathies / Q000188:drug therapy* / Q000378:metabolism; D004359:Drug Therapy, Combination; D007668:Kidney / Q000033:anatomy & histology / Q000502:physiology; D008099:Liver / Q000378:metabolism; D009929:Organ Size; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D024508:Tocotrienols / Q000097:blood / Q000494:pharmacology; D024341:Xanthophylls / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000097:blood / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D024508:Tocotrienols; D024341:Xanthophylls; C005948:astaxanthine; D024502:alpha-Tocopherol; D001205:Ascorbic Acid