Use of plasma DNA to predict mortality and need for intensive care in patients with abdominal pain.
BACKGROUND
We investigated the value of plasma deoxyribonucleic acid concentrations in patients presenting with acute abdominal pain to predict need for intensive care or mortality.


METHODS
Plasma deoxyribonucleic acid taken from patients with acute abdominal pain was analyzed for the beta-globin gene using the quantitative polymerase chain reaction. The primary outcome measure was the combined 28-day mortality or admission to the intensive care unit.


RESULTS
Of 287 consecutive patients with acute abdominal pain recruited, 12 patients were admitted to the intensive care unit and/or died. Median plasma DNA concentrations were higher in patients with cancer and major organ inflammation. Mean plasma DNA concentrations were three-fold higher in patients with systemic inflammatory response syndrome, five-fold higher in patients who died within 28 days, and eight-fold higher in patients admitted to the intensive care unit. The area under the receiver operator curve for plasma DNA concentrations and intensive care unit admission/mortality was 0.804. At a cut-off of 1100 GE/ml, the sensitivity was 67% (95%CI 35-90) and specificity was 89% (95%CI 84-92). At a cut-off of 175 GE/ml, the sensitivity was 100% (95%CI 73-100) and specificity was 30% (95%CI 25-36). Plasma DNA concentration predicted need for intensive care unit admission or death (adjusted odds ratio 1.4; P<0.0001).


CONCLUSIONS
Plasma DNA may have a role in patients with acute abdominal pain as a marker for inflammation and cancer, and a predictor of intensive care unit admission/mortality.
Rainer
Timothy H
TH
Accident and Emergency Medicine Academic Unit, Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, China. thrainer@cuhk.edu.hk
Chan
Allen K C
AK
Lee
Larry L Y
LL
Yim
Veronica W T
VW
Lam
Nicole Y L
NY
Yeung
S W
SW
Graham
Colin A
CA
Lo
Dennis Y M
DY
D015746:Abdominal Pain / Q000235:genetics* / Q000401:mortality*; D000208:Acute Disease; D000328:Adult; D000368:Aged; D003422:Critical Care / Q000706:statistics & numerical data*; D004247:DNA / Q000097:blood* / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007249:Inflammation / Q000097:blood; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood; D010949:Plasma / Q000737:chemistry; D011237:Predictive Value of Tests; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016896:Treatment Outcome; D055544:beta-Globins / Q000235:genetics
D055544:beta-Globins; D004247:DNA