Schedule-induced polydipsia in lines of rats selectively bred for high and low ethanol preference.
Ethanol drinking was assessed in the P/NP, HAD1/LAD1, and HAD2/LAD2 lines of rats under environmental conditions that produce schedule-induced polydipsia. Female rats (n = 8/line), maintained at 85% of free-feeding body weights, underwent daily 1-h sessions during which 45-mg food pellets were delivered every 60 s. Water, 2, 4, 8, 16, or 32% w/v ethanol solution was available from a single bottle for 8 consecutive sessions at each concentration, with blood-ethanol levels (BELs) determined after selected sessions. P and HAD2 rats drank more water and ethanol than their non-preferring counterparts, while HAD1 and LAD1 rats did not differ. Ethanol intake and BELs were positively correlated (r = 0.75) across lines. Finally, rats were allowed 14 daily choice sessions with 8% ethanol and water concurrently available. Water intake generally exceeded ethanol intake in all lines, while P rats drank similar amounts of both fluids. These line differences indicate pleiotropic effects of genes that mediate ethanol intake and schedule-induced behaviors.
Gilpin
N W
NW
Department of Psychology, Program in Psychobiology of Addictions, Purdue School of Science, Indiana University/Purdue University at Indianapolis, Indianapolis, IN 46202, USA. nickg@scripps.edu
Badia-Elder
N E
NE
Elder
R L
RL
Stewart
R B
RB
D000428:Alcohol Drinking / Q000235:genetics*; D000438:Alcohols / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal; D001835:Body Weight; D002755:Choice Behavior / Q000187:drug effects; D003216:Conditioning, Operant / Q000187:drug effects; D004326:Drinking / Q000187:drug effects / Q000235:genetics; D004777:Environment; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D051381:Rats; D017208:Rats, Wistar; D013894:Thirst; D013997:Time Factors
D000438:Alcohols; D000431:Ethanol
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