[Regional lymph nodes targetting with enrichment of polyphase liposome].
OBJECTIVE
To investigate the feasibility of regional lymph nodes targetting with enrichment of radioactive 99mTc-polyphase liposome of 5-fluorouracil (99mTc-FL, FL).


METHODS
18 rabbits were randomly divided into 3 groups, 6 rabbits per group. All rabbits were injected hypodermally with of 99mTc-FL in the right and left big toe webs, 18.5 MBq each side. The post-injection interval was 3 h in group 1, 6 h in group 2, and 8 h in group 3. The radioactivity was examined in the resected local lymph nodes, non-draining lymph nodes, liver, spleen, kidney, heart, lung, intestines, and in blood and urine.


RESULTS
The radioactive isotope uptake percentage (%) was 2.32 +/- 0.75 in group 1, 5.37 +/- 1.73 in group 2, 8.61 +/- 1.89 in group 3. The radioactive isotope uptake percentage (%) per gram in local lymph nodes was significantly different between each two groups among the 3 groups (P < 0.05). The ratios of x of regional lymph nodes/non-draining lymph nodes, regional lymph nodes/blood, regional lymph nodes/urine, regional lymph nodes/liver, regional lymph nodes/spleen, regional lymph nodes/kidney, regional lymph nodes/heart, regional lymph nodes/lung, regional lymph nodes/intestine in group 1 were 232.00, 16.57, 23.20, 29.00, 19.33, 25.78, 46.40, 46.40 and 25.78, respectively. The ratios in group 2 were 89.50, 41.31, 18.52, 67.13, 41.31, 25.57, 134.25, 59.67 and 59.67, respectively. The ratios in group 3 were 86.10, 61.50, 16.56, 53.81, 57.40, 10.01, 107.63, 107.63 and 86.10, respectively. The differences of radioactive isotope uptake percentage were statistically significant (P < 0.01) between regional lymph nodes and other organs, i. e. non-draining lymph nodes, blood, urine, liver, spleen, kidney, heart, lung and intestine per gram in each group.


CONCLUSIONS
The radioactive 99mTc-FL may slowly flow into regional lymphatic chains rather than directly enter blood circulation. So 99mTc-FL can be highly accumulated in the local lymph nodes. This regional lymph nodes targetting with enrichment of radioactive 99mTc-FL evidently indicates the feasibility of regional lymph system chemotherapy for pulmonary malignancies.
Li
Xiao-Hai
XH
Department of Thoracic and Cardiovascular Surgery, Guangzhou Institute of Respiratory Diseases,Guangzhou 510120, China.
He
Jian-Xing
JX
Chen
Ping
P
Qiang
Yong-Gan
YG
Wei
Xian-Zhong
XZ
Zhang
Gui-Ping
GP
Hua
Nin
N
D000818:Animals; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D006321:Heart / Q000000981:diagnostic imaging; D007422:Intestines / Q000000981:diagnostic imaging; D007668:Kidney / Q000000981:diagnostic imaging; D008081:Liposomes; D008099:Liver / Q000000981:diagnostic imaging; D008168:Lung / Q000000981:diagnostic imaging; D008198:Lymph Nodes / Q000000981:diagnostic imaging*; D008297:Male; D015609:Organotechnetium Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics; D011817:Rabbits; D011877:Radionuclide Imaging; D011897:Random Allocation; D013154:Spleen / Q000000981:diagnostic imaging
D008081:Liposomes; D015609:Organotechnetium Compounds; C532874:technetium 99m 5-fluorouracil; D005472:Fluorouracil