Synthesis of four colors fluorescently labelled 3'-O-blocked nucleotides with fluoride cleavable blocking group and linker for array based Sequencing-by-Synthesis applications.
Reversible terminators having a fluoride cleavable 3'-O-blocking group are presented. Each nucleotide triphosphate is labelled by a fluorescent dye cleavable by the same reagent. We present here their synthesis, cleavage experiments and polymerase incorporation tests for a possible use in a process of Sequencing-by-Synthesis.
Knapp
Diana C
DC
Institut für Organische Chemie und Chemische Biologie, J. W. Goethe Universität Frankfurt am Main, Max-von-Laue Str. 7, D-60438 Frankfurt am Main, Germany.
Keller
Angelika
A
D'Onofrio
Jennifer
J
Lubys
Arvydas
A
Serva
Saulius
S
Kurg
Ants
A
Remm
Maido
M
Kwiatkowski
Marek
M
Engels
Joachim W
JW
D003116:Color; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA
D005456:Fluorescent Dyes; D009711:Nucleotides; D004259:DNA-Directed DNA Polymerase; D005459:Fluorides