Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling.
The objective of this study was to identify QTL for growth rate in the blacklip abalone Haliotis rubra using selective DNA pooling. Three full-sibling families of H. rubra derived from crosses of wild broodstock were used. DNA was extracted from the largest and smallest 10% of progeny and combined into two pools for each phenotypic tail. The DNA pools were typed with 139 microsatellites, and markers showing significant differences between the peak height ratios of alleles inherited from the parents were individually genotyped and analysed by interval mapping. A strong correlation (r = 0.94, P < 0.001) was found between the t-values from the analysis of pools and the t-values from the analysis of individual genotypes. Based on the interval mapping analysis, QTL were detected on nine linkage groups at a chromosome-wide P < 0.01 and one linkage group at a chromosome-wide P < 0.05. The study demonstrated that selective DNA pooling is efficient and effective as a first-pass screen for the discovery of QTL in an aquaculture species.
Baranski
M
M
Biosciences Research Division, Department of Primary Industries, Attwood, Vic. 3049, Australia. matthew.baranski@nofima.no
Rourke
M
M
Loughnan
S
S
Hayes
B
B
Austin
C
C
Robinson
N
N
D000818:Animals; D004247:DNA / Q000235:genetics*; D005260:Female; D049851:Gastropoda / Q000235:genetics / Q000254:growth & development*; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction; D040641:Quantitative Trait Loci
D005819:Genetic Markers; D004247:DNA