title: Ovine progressive pneumonia provirus levels associate with breed and Ovar-DRB1.
abstract: Previous studies initiated defining the role of host genetics in influencing the outcome of exposure to ovine progressive pneumonia virus. However, specific genes influencing host control of virus replication and disease progression have not been identified. This study, using 383 ewes of the Columbia, Polypay, and Rambouillet breeds, tested the hypothesis that host control of OPPV as measured by provirus levels in the peripheral blood associates with certain breeds and MHC class II Ovis aries (Ovar)-DRB1 expressed alleles. Rambouillet ewes were less likely to have measurable provirus levels as compared to Columbia ewes at ages 5 and 6 (P value < 0.02), and they exhibited lower provirus levels when compared to both Columbia and Polypay ewes of the same ages (P value < 0.05). The presence of DRB1*0403- or DRB1*07012-expressed alleles were significantly associated (P value = 0.019 and 0.0002, respectively) with lower OPP provirus levels but only were only found in 11% of the ewe flock. Analysis of each segregating amino acid in the beta1 domain of DR beta-chain revealed that amino acids Y31, T32, N37, T51, Q60, or N74 significantly associated (P value range = 0.0003-0.018) with lower OPP provirus levels, whereas amino acids H32, A38, or I67 associated (P value range = 0.013-0.043) with higher OPP provirus levels. These results suggest that Ovar-DRB1 contributes as one host genetic factor that controls OPP provirus levels, but does not fully account for the breed-specific OPP proviral differences.
authors.affiliation: US Department of Agriculture, Animal Disease Research Unit, Agricultural Research Service, Pullman, WA 99164-6630, USA. lherrman@vetmed.wsu.edu
authors.forename: Lynn M
authors.initials: LM
authors.lastname: Herrmann-Hoesing
authors.forename: Stephen N
authors.initials: SN
authors.lastname: White
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Mousel
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Lewis
authors.forename: Donald P
authors.initials: DP
authors.lastname: Knowles
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D005787:Gene Frequency; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D054884:Host-Pathogen Interactions; D010802:Phylogeny; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D011533:Proviruses / Q000302:isolation & purification*; D016415:Sequence Alignment; D017385:Sequence Homology; D012756:Sheep / Q000235:genetics* / Q000276:immunology; D012757:Sheep Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D013045:Species Specificity; D019562:Viral Load; D014766:Viremia / Q000235:genetics / Q000276:immunology; D016662:Virus Integration; D014779:Virus Replication; D014790:Visna-maedi virus / Q000302:isolation & purification*
chemical_list: D000949:Histocompatibility Antigens Class II
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