title: Northern analysis of viral plus- and minus-strand RNAs.
abstract: Replication is a fundamental activity of viruses. Replication of positive-sense RNA viruses involves the synthesis of complementary minus-strand intermediates from the parental RNA template followed by synthesis of nascent plus strands. Negative-sense RNA genome and double-stranded RNA are copied into positive-sense mRNA before translation. To detect and estimate the abundance of plus- and minus-strand viral transcripts in the infected samples, northern analysis is the most commonly used method.
authors.affiliation: Institute of Plant and Microbial Biology, Taipei, Taiwan.
authors.forename: Paramasivan Vijaya
authors.initials: PV
authors.lastname: Palani
authors.forename: Na-Sheng
authors.initials: NS
authors.lastname: Lin
mesh_terms: D015152:Blotting, Northern / Q000379:methods*; D010935:Plant Diseases / Q000821:virology; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014026:Nicotiana / Q000821:virology; D014779:Virus Replication
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral