title: Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea.
abstract: In adult planarians, the replacement of cells lost to physiological turnover or injury is sustained by the proliferation and differentiation of stem cells known as neoblasts. Neoblast lineage relationships and the molecular changes that take place during differentiation into the appropriate cell types are poorly understood. Here we report the identification and characterization of a cohort of genes specifically expressed in neoblasts and their descendants. We find that genes with severely downregulated expression after irradiation molecularly define at least three discrete subpopulations of cells. Simultaneous BrdU labeling and in situ hybridization experiments in intact and regenerating animals indicate that these cell subpopulations are related by lineage. Our data demonstrate not only the ability to measure and study the in vivo population dynamics of adult stem cells during tissue homeostasis and regeneration, but also the utility of studies in planarians to broadly inform stem cell biology in adult organisms.
authors.affiliation: Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
authors.forename: George T
authors.initials: GT
authors.lastname: Eisenhoffer
authors.forename: Hara
authors.initials: H
authors.lastname: Kang
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sánchez Alvarado
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D015536:Down-Regulation / Q000528:radiation effects; D020869:Gene Expression Profiling; D010932:Planarians / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology*
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