title: PACemakers of proteasome core particle assembly.
abstract: The 26S proteasome mediates ubiquitin-dependent proteolysis in eukaryotic cells. A number of studies including very recent ones have revealed that assembly of its 20S catalytic core particle is an ordered process that involves several conserved proteasome assembly chaperones (PACs). Two heterodimeric chaperones, PAC1-PAC2 and PAC3-PAC4, promote the assembly of rings composed of seven alpha subunits. Subsequently, beta subunits join to form half-proteasome precursor complexes containing all but one of the 14 subunits. These complexes lack the beta7 subunit but contain UMP1, another assembly chaperone, and in yeast, at least to some degree, the activator protein Blm10. Dimerization of two such complexes is triggered by incorporation of beta7, whose C-terminal extension reaches out into the other half to stabilize the newly formed 20S particle. The process is completed by the maturation of active sites and subsequent degradation of UMP1 and PAC1-PAC2.
authors.affiliation: Departamento de Química, Bioquímica e Farmácia, Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8000-117 Faro, Portugal. pcramos@ualg.pt
authors.forename: Paula C
authors.initials: PC
authors.lastname: Ramos
authors.forename: R Jürgen
authors.initials: RJ
authors.lastname: Dohmen
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D019281:Dimerization; D019143:Evolution, Molecular; D006801:Humans; D008954:Models, Biological; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism / Q000502:physiology
chemical_list: D018832:Molecular Chaperones; D046912:Multiprotein Complexes; D021122:Protein Subunits; C110856:proteasome maturation protein; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease