Functional consequences of hippocampal neuronal ectopia in the apolipoprotein E receptor-2 knockout mouse.
Little is known about the impact ectopically located neurons have on the functional connectivity of local circuits. The ApoER2 knockout mouse has subtle cytoarchitectural disruptions, altered prepulse inhibition, and memory abnormalities. We evaluated this mouse mutant as a model to study the role ectopic neurons play in the manifestation of symptoms associated with brain diseases. We found that ectopic CA1 pyramidal and inhibitory neurons in the ApoER2 knockout hippocampus are organized into two distinct stratum pyramidale layers. In vitro analyses found that ApoER2 is not required for neurons to reach maturity in regard to dendritic arborization and synaptic structure density, and electrophysiological testing determined that neurons in both strata pyramidale are integrated into the hippocampal network. However, the presence of these two layers alters the spatiotemporal pattern of hippocampal activity, which may explain why ApoER2 knockout mice have selective cognitive dysfunctions that are revealed only under challenging conditions.
Fish
Kenneth N
KN
Department of Psychiatry, Western Psychiatric Institute and Clinic, University of Pittsburgh School of Medicine, Biomedical Science Tower, Pittsburgh, PA 15213, USA. fishkn@umpc.edu
Krucker
Thomas
T
D000818:Animals; D002478:Cells, Cultured; D003712:Dendrites / Q000502:physiology / Q000648:ultrastructure; D019706:Excitatory Postsynaptic Potentials; D006624:Hippocampus / Q000002:abnormalities* / Q000473:pathology / Q000503:physiopathology*; D026502:LDL-Receptor Related Proteins; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009474:Neurons / Q000473:pathology* / Q000502:physiology; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000473:pathology* / Q000502:physiology; D011973:Receptors, LDL / Q000378:metabolism; D018110:Receptors, Lipoprotein / Q000235:genetics* / Q000378:metabolism
D026502:LDL-Receptor Related Proteins; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; C036053:VLDL receptor; C098773:low density lipoprotein receptor-related protein 8
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