Preparation and properties of a novel drug delivery system with both magnetic and biomolecular targeting.
By loading doxorubicin (DOX) on 5-carboxyl-fluorescein (FAM) labeled AGKGTPSLETTP peptide (A54) coupled starch-coated iron oxide nanoparticles (SIONs), we prepared a novel aqueous drug delivery system with both magnetic and biomolecular targeting, which was specific to human hepatocellular carcinoma cell line BEL-7402. The saturated extent of adsorption reached 2.0 mg DOX/mg A54-SIONs at 28 degrees C, which provided a rather high dose of DOX loading for application. Tests in vitro demonstrated the specificity of DOX-loaded A54-SIONs to BEL-7402 cells. The microscopy images proved that DOX-loaded A54-SIONs were successfully targeted to tumor tissue of nude mice with an external magnetic field in vivo. MTT assay showed higher cytostatic effect of DOX-loaded A54-SIONs to hepatocellular carcinoma cells BEL-7402 than that of DOX-loaded SIONs.
Yang
Yong
Y
Department of Physics, East China Normal University, Shanghai, 200062, People's Republic of China.
Jiang
Ji-Sen
JS
Du
Bing
B
Gan
Zhi-Feng
ZF
Qian
Min
M
Zhang
Ping
P
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000737:chemistry / Q000302:isolation & purification; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage; D004337:Drug Carriers / Q000737:chemistry / Q000302:isolation & purification*; D016503:Drug Delivery Systems; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology; D008280:Magnetics; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D009368:Neoplasm Transplantation; D009842:Oligopeptides / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014183:Transplantation, Heterologous
D000903:Antibiotics, Antineoplastic; D001672:Biocompatible Materials; D004337:Drug Carriers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009842:Oligopeptides; C045132:4-carboxyfluorescein; D004317:Doxorubicin
J Biomed Mater Res A. 2008 Jan;84(1):10-8
17600321
J Med Chem. 2001 Nov 22;44(24):4216-24
11708923
Acta Biomater. 2007 Mar;3(2):233-42
17224313
Cancer Res. 1984 Feb;44(2):613-8
6692366
Acta Biomater. 2008 Jan;4(1):40-8
17681499
Cancer Res. 1994 Nov 1;54(21):5670-4
7923215
Curr Opin Chem Biol. 2002 Jun;6(3):399-404
12023122
Mol Pharm. 2006 Jul-Aug;3(4):415-23
16889435
Mol Pharm. 2005 May-Jun;2(3):194-205
15934780
Cancer Res. 1989 Aug 15;49(16):4373-84
2545340
Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12617-21
12235356
Int J Hyperthermia. 1995 Mar-Apr;11(2):211-6
7790735
J Am Chem Soc. 2006 Mar 22;128(11):3472-3
16536492
Biomaterials. 2005 Jun;26(18):3995-4021
15626447
Biopolymers. 2002;66(3):184-99
12385037
Nature. 1996 Mar 28;380(6572):364-6
8598934
Langmuir. 2005 May 10;21(10):4262-5
16032831
Nano Lett. 2006 Nov;6(11):2427-30
17090068
J Med Chem. 2003 Jul 17;46(15):3403-11
12852770
Langmuir. 2005 Sep 13;21(19):8858-64
16142971