Analysis of eicosanoids and related lipid mediators using mass spectrometry.
Eicosanoids are oxidized arachidonate-derived lipids products generated by lipoxygenase, cyclo-oxygenase and cytochrome P450. Their bioactivity is mediated via receptor-dependent mechanisms and they are involved in a diverse array of processes in both health and disease. For many years, GC-MS was the method of choice for eicosanoid analysis, but, more recently, the availability of high-sensitivity electrospray LC (liquid chromatography)-MS/MS (tandem MS) has provided a new approach for quantification, while minimizing sample preparation. The present review summarizes the various methods available for routine quantification of eicosanoids, focusing ultimately on their analysis using a hybrid Q-Trap mass spectrometer.
Maskrey
Benjamin H
BH
Department of Medical Biochemistry and Immunology, Cardiff University, Heath Park, Cardiff CF14 4XN, UK.
O'Donnell
Valerie B
VB
D000818:Animals; D015777:Eicosanoids / Q000032:analysis*; D015227:Lipid Peroxidation; D008055:Lipids / Q000032:analysis*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure
D015777:Eicosanoids; D008055:Lipids