[Characterization and antibacterial effect of Ag-nHA-nTiO2/polyamide 66 nanocomposite membrane on oral bacteria].
OBJECTIVE
Undried silver-hydroxyapatite-titania (Ag-nHA-nTiO2) nanoparticles slurry was used to make membrane with polyamide 66 (PA66) by co-polymerization method. The purpose of this study is to test the physical and chemical characteristics and antibacterial ability.


METHODS
The morphology, chemical components and structures of the membrane were characterized by atomic absorption spectrometer (AAS), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy-dispersive X-ray analysis (EDX). Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Porphyromonas gingivalis (P. gingivalis), Fusobacterium nucleatum (F. nucleatum) and Streptococcus mutans (S. mutans) were utilized to test the antibacterial effect.


RESULTS
XRD results demonstrated that the membrane have characteristic diffraction peaks of pure hydroxyapatite (HA). A homogeneous distribution of the Ca, P, Ti and Ag element in the membrane was confirmed by EDX. Both surface and section showed porous structure which was confirmed by SEM and the average hole size was 20-30 microm. The bacteria assay reflected to the antibacterial effect, 50.10% of S. aureus and 56.31% of E. coli were killed. However, 91.84% of P. gingivalis, 90.64% of F. nucleatum and 90.49% of S. mutans were killed and pictures of SEM showed obviously fewer cells on the surface.


CONCLUSIONS
The nanocomposite membrane could be one of the bioactive materials with antibacterial properties for oral guided bone regeneration technique.
Bai
Shi
S
Dept. of Implantology, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, China.
Mo
An-chun
AC
Xian
Su-qin
SQ
Zuo
Yi
Y
Li
Yu-bao
YB
Xu
Wei
W
D000900:Anti-Bacterial Agents; D001861:Bone Regeneration; D017886:Durapatite; D004926:Escherichia coli; D053761:Nanocomposites; D009757:Nylons; D012834:Silver; D013211:Staphylococcus aureus; D014025:Titanium; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D009757:Nylons; C009495:titanium dioxide; D012834:Silver; D017886:Durapatite; D014025:Titanium