title: Mouse gammadelta T cells are capable of expressing MHC class II molecules, and of functioning as antigen-presenting cells.
abstract: Although human and bovine gammadelta T cells were shown to express MHC class II antigen and function as APCs, attempts to determine if mouse gammabeta T cells have similar functions remained unsuccessful. We now show that gammadelta T cells derived from immunized mice also can be induced to express MHC class II and co-stimulatory molecules after activation in vitro, and are capable of antigen presentation. Using highly purified gammadelta T cells, we found that, unlike human gammadelta T cells, the expression of MHC class II molecules by mouse gammadelta T cells is limited to newly activated cells. Highest levels of MHC class II expression were seen on activated gammadelta T cells that had lost most surface-expressed gammadelta TCR while exhibiting increased levels of intracellular gammadelta TCR. In the absence of further stimulation, MHC class II expression gradually declined with the gammadelta T cells regaining their surface TCR. We also show that cytokine-activated gammadelta T cells can also express MHC class II antigen and exercise antigen-presenting activity.
authors.affiliation: Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY, USA.
authors.forename: Lan
authors.initials: L
authors.lastname: Cheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.forename: Hui
authors.initials: H
authors.lastname: Shao
authors.forename: Gencheng
authors.initials: G
authors.lastname: Han
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.forename: Yafei
authors.initials: Y
authors.lastname: Huang
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: O'Brien
authors.forename: Willi K
authors.initials: WK
authors.lastname: Born
authors.forename: Henry J
authors.initials: HJ
authors.lastname: Kaplan
authors.forename: Deming
authors.initials: D
authors.lastname: Sun
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002469:Cell Separation; D015536:Down-Regulation / Q000276:immunology; D005136:Eye Proteins / Q000276:immunology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D066298:In Vitro Techniques; D020381:Interleukin-17 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000378:metabolism; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D005136:Eye Proteins; D000949:Histocompatibility Antigens Class II; D020381:Interleukin-17; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012177:Retinol-Binding Proteins; D051193:Toll-Like Receptors; D000099214:Retinol-Binding Proteins, Interstitial
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