Functional characterization of sll0659 from Synechocystis sp. PCC 6803.
Synechocystis sp. PCC 6803 lacks a gene for the any known types of lycopene cyclase. Recently, we reported that Sll0659 (unknown for its function) from Synechocystis sp. PCC6803 shows similarity in sequence to a lycopene cyclase gene-CruA from Chlorobium tepidum. To test, whether sll0659 encoded protein serves as lycopene cyclase, in this study, we investigated the carotenoids of the wild types and mutants. In the sll0659 deleted mutant, there is no blockage at the lycopene cyclization step. Our results demonstrate that sll0659 does not affect lycopene cycilzation. However, the ultrastructure of mutants suggests the involvement or necessity of sll0659 in the cell division.
Liang
Cheng-Wei
CW
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R. China.
Zhang
Xiao-Wen
XW
Tian
Li
L
Qin
Song
S
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D041883:Chlorobium / Q000201:enzymology* / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D003500:Cyclization; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D016133:Polymerase Chain Reaction; D046939:Synechocystis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D019207:beta Carotene / Q000378:metabolism
D001426:Bacterial Proteins; D019207:beta Carotene; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase