Influence of storage media containing Salvia officinalis on survival of periodontal ligament cells.
OBJECTIVE
The purpose of this study was to determine the ability of Salvia officinalis (S. officinalis) extracts to serve as a storage medium for the maintenance of periodontal ligament (PDL) cell viability of avulsed teeth.


METHODS
PDL cells were obtained from healthy third molars and cultured in Dulbecco's Modi?ed Eagle's Medium (DMEM). Cultures were subjected to 4, 2.5, 1.5, and 0.5% S. officinalis solutions, Hank's balanced salt solution (HBSS), phosphate buffered saline (PBS), and tap water. Tissue culture plates were incubated with experimental media at 37 masculineC for 1, 3, 6, 12 or 24 hours. PDL cell viability was assessed by trypan blue exclusion. Statistical analysis of the data was performed by one-way analysis of variance (ANOVA) complemented by the Tukey's test. The level of significance was 5% (p< 0.05).


RESULTS
The results showed 2.5% S. officinalis was a more effective storage medium than the other experimental solutions (p<0.05). Only at 1 hour and 3 hours was there found similar effect between 2.5% S. officinalis and HBSS. At 24 hours, 2.5% S. officinalis was found to be significantly better than the other solutions tested.


CONCLUSIONS
S. officinalis can be recommended as a suitable transport medium for avulsed teeth.


CONCLUSIONS
The findings of this study support the use of S. officinalis as another option for clinicians to use to store and transport avulsed teeth until reimplantation procedures can be done.
Ozan
Fatih
F
Department of Oral and Maxillofacial Surgery, Cumhuriyet University, Sivas, Turkey. fozan@cumhuriyet.edu.tr
Polat
Zübeyde Akin
ZA
Tepe
Bektaş
B
Er
Kürşat
K
D000704:Analysis of Variance; D002470:Cell Survival; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D010513:Periodontal Ligament / Q000166:cytology / Q000502:physiology*; D010936:Plant Extracts; D018515:Plant Leaves; D027543:Salvia officinalis; D014084:Tooth Avulsion / Q000628:therapy*
C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D010936:Plant Extracts