title: No relationship exists between PBP 2a amounts expressed in different MRSA strains obtained clinically and their beta-lactam MIC values.
abstract: After establishing a linear relationship between the amount of penicillin-binding protein (PBP) 2a and membrane proteins of methicillin-resistant Staphylococcus aureus (MRSA) COL by dot-blot analysis using an antibody against PBP 2a, we determined the PBP 2a quantities in membrane fractions prepared from 14 different MRSA cells. Methicillin-sensitive S. aureus ATCC 6538P was used as a quality control strain. The amounts of PBP 2a diverged among the strains, and no relationship to beta-lactam MIC values were observed in the corresponding strains.
authors.affiliation: Department of Molecular Cell Biology and Medicine, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Md Anowar Khasru
authors.initials: MA
authors.lastname: Parvez
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shibata
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Nakano
authors.forename: Shingo
authors.initials: S
authors.lastname: Niimi
authors.forename: Nobuo
authors.initials: N
authors.lastname: Fujii
authors.forename: Naokatu
authors.initials: N
authors.lastname: Arakaki
authors.forename: Tomihiko
authors.initials: T
authors.lastname: Higuti
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D016106:Methicillin Resistance; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D047090:beta-Lactams / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; D047090:beta-Lactams; C487789:mecA protein, Staphylococcus aureus