title: Application of the immunoperoxidase technique FDR the detection of Derzsy's disease virus antigen in cell culture and goslings.
abstract: Application of a direct immunoperoxidase technique for the detection of Derzsy's disease virus antigen in cell culture and goslings is described. Anti-virus globulins were labelled with horseradish peroxidase by use of sodium periodate as a coupling agent. Virus antigen was detected in the nuclei of goose embryo fibroblasts from 48 to 72 hours post-inoculation and in the nuclei of hepatic cells between 5 to 8 days after infection. Microstructural detail was much better in cell culture than in the liver.
authors.affiliation: Veterinary Research Institute, 24-100 Pulawy, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Roszkowski
authors.forename: P
authors.initials: P
authors.lastname: Gaździński
authors.forename: W
authors.initials: W
authors.lastname: Kozaczyński
authors.forename: M
authors.initials: M
authors.lastname: Bartoszcze