Regenerative potentials of the murine thyroid in experimental autoimmune thyroiditis: role of CD24.
Hashimoto thyroiditis can be partially reproduced in mice by immunization with thyroglobulin or, more recently, thyroperoxidase. This experimental autoimmune thyroiditis (EAT) model has been extensively characterized during early disease phases (up to d 35 after immunization). By extending the analysis of EAT to 100 d after immunization, we noted a remarkable regenerative capacity of the thyroid and the expression of Oct-4, suggesting in vivo the existence of adult thyroid stem cells. After an almost complete destruction of the follicular architecture, occurring between d 21 and 28, the thyroid was capable of restoring its follicles and reducing the mononuclear infiltration, so that by d 100 after immunization, it regained its normal morphology and function. During this regeneration process, thyrocytes expressed high levels of CD24. We therefore assessed the role of CD24 in thyroid regeneration by inducing EAT in mice lacking CD24. Regeneration was faster in the absence of CD24, likely a consequence of the effect of CD24 on the infiltrating lymphocytes. The study suggests that the EAT model can also be used as a tool to investigate adult thyroid stem cells.
Chen
Cindy Y
CY
Department of Pathology, The Johns Hopkins Medical Institutions, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.
Kimura
Hiroaki
H
Landek-Salgado
Melissa A
MA
Hagedorn
Judith
J
Kimura
Miho
M
Suzuki
Koichi
K
Westra
William
W
Rose
Noel R
NR
Caturegli
Patrizio
P
D000818:Animals; D051927:CD24 Antigen / Q000502:physiology*; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008811:Mice, Inbred DBA; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D012038:Regeneration; D013954:Thyroglobulin / Q000378:metabolism; D013961:Thyroid Gland / Q000473:pathology / Q000503:physiopathology*; D013967:Thyroiditis, Autoimmune / Q000473:pathology / Q000503:physiopathology*; D013974:Thyroxine / Q000378:metabolism
D051927:CD24 Antigen; D018809:Proliferating Cell Nuclear Antigen; D013954:Thyroglobulin; D013974:Thyroxine
J Immunol. 2002 Mar 1;168(5):2470-4
11859140
J Endocrinol. 2008 Sep;198(3):471-87
18550786
Autoimmun Rev. 2003 May;2(3):119-25
12848952
Thyroid. 2003 May;13(5):419-26
12855008
N Engl J Med. 2003 Aug 7;349(6):570-82
12904523
J Mol Histol. 2004 Mar;35(3):255-62
15339045
J Exp Med. 2004 Oct 18;200(8):1083-9
15477346
J Immunol. 1968 Aug;101(2):242-50
5668024
Proc Soc Exp Biol Med. 1968 Nov;129(2):412-6
5723648
Science. 1971 Dec 10;174(4014):1137-9
5133731
Eur J Immunol. 1978 Aug;8(8):539-51
81133
Clin Exp Immunol. 1985 Aug;61(2):257-64
4042424
Clin Immunol Immunopathol. 1989 Nov;53(2 Pt 1):254-67
2791341
Eur J Immunol. 1990 Jul;20(7):1597-602
1696896
J Immunol. 1990 Sep 15;145(6):1952-9
2118158
Eur J Immunol. 1991 Jun;21(6):1397-402
2044653
Physiol Rev. 1992 Jul;72(3):667-97
1320763
Acta Neuropathol. 1993;86(3):275-84
8213086
Eur J Immunol. 1994 Mar;24(3):731-7
8125140
Virchows Arch. 1994;425(4):399-406
7820302
Int Immunol. 1995 Oct;7(10):1557-65
8562500
Mol Biol Rep. 1995;21(3):129-40
8832901
Clin Immunol Immunopathol. 1997 Sep;84(3):223-43
9281381
J Immunol. 1956 Jun;76(6):417-27
13332243
Endocrinology. 2005 Mar;146(3):1154-62
15591134
J Exp Med. 2006 Jul 10;203(7):1713-20
16769998
Thyroid. 2006 Jun;16(6):537-44
16839255
J Leukoc Biol. 2007 Jan;81(1):306-14
17046971
Am J Pathol. 2007 Mar;170(3):875-87
17322373
Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):6019-24
17389381
J Immunol. 2007 May 15;178(10):6227-35
17475850
Trends Immunol. 2007 Jul;28(7):315-20
17531534
Cell Tissue Res. 2007 Sep;329(3):457-67
17522896
Endocrinology. 2007 Sep;148(9):4251-8
17584961
J Clin Endocrinol Metab. 2007 Sep;92(9):3681-8
17609303
Endocrinology. 2007 Dec;148(12):5734-45
17823262
Cancer Res. 2008 Apr 15;68(8):2803-12
18413748
Am J Pathol. 2008 Jun;172(6):1591-602
18467701
Mod Pathol. 2003 Jan;16(1):43-8
12527712