title: Comparison of transcription of multiple genes during mycelia transition to yeast cells of Paracoccidioides brasiliensis reveals insights to fungal differentiation and pathogenesis.
abstract: The ascomycete Paracoccidioides brasiliensis is a human pathogen with a broad distribution in Latin America. The infection process of P. brasiliensis is initiated by aerially dispersed mycelia propagules, which differentiate into the yeast parasitic phase in human lungs. Therefore, the transition to yeast is an initial and fundamental step in the infective process. In order to identify and characterize genes involved in P. brasiliensis transition to yeast, which could be potentially associated to early fungal adaptation to the host, expressed sequence tags (ESTs) were examined from a cDNA library, prepared from mycelia ongoing differentiation to yeast cells. In this study, it is presented a screen for a set of genes related to protein synthesis and to protein folding/modification/destination expressed during morphogenesis from mycelium to yeast. Our analysis revealed 43 genes that are induced during the early transition process, when compared to mycelia. In addition, eight novel genes related to those processes were described in the P. brasiliensis transition cDNA library. The types of induced and novel genes in the transition cDNA library highlight some metabolic aspects, such as putative increase in protein synthesis, in protein glycosylation, and in the control of protein folding that seem to be relevant to the fungal transition to the parasitic phase.
authors.affiliation: Laboratório de Biologia Molecular, ICB II, Campus II, Universidade Federal de Goiás, 74001-970 Goiânia, Goiás, Brazil.
authors.forename: Juliana Alves
authors.initials: JA
authors.lastname: Parente
authors.forename: Clayton Luiz
authors.initials: CL
authors.lastname: Borges
authors.forename: Alexandre Melo
authors.initials: AM
authors.lastname: Bailão
authors.forename: Maria Sueli S
authors.initials: MS
authors.lastname: Felipe
authors.forename: Maristela
authors.initials: M
authors.lastname: Pereira
authors.forename: Célia Maria
authors.initials: CM
authors.lastname: de Almeida Soares
mesh_terms: D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D006801:Humans; D009024:Morphogenesis / Q000502:physiology*; D025282:Mycelium / Q000235:genetics / Q000254:growth & development*; D010228:Paracoccidioides / Q000166:cytology / Q000254:growth & development / Q000472:pathogenicity; D014176:Protein Biosynthesis; D017510:Protein Folding; D014158:Transcription, Genetic; D015003:Yeasts / Q000166:cytology* / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins
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