Transcriptional analysis of the two reverse gyrase encoding genes of Sulfolobus solfataricus P2 in relation to the growth phases and temperature conditions.
Sulfolobus solfataricus, a hyperthermophilic crenarchaeon, contains two genes encoding reverse gyrases, topR1 and topR2. The steady-state level of their transcripts were quantified during the growth phases for cells maintained either at 72, or 80 degrees C, and after temperature changes from one to the other temperature. The transcripts of both genes are weakly expressed, but the highest level is observed in actively dividing cells, and is almost undetectable in cells in decline phase. During the temperature shift experiments, there is no significant topR2 variation. By contrast, there is a maximum 2.4-fold increase in topR1 transcripts within 30 min after the downshift. After 1 h, the transcript level reaches the level characteristic of cells adapted to the new temperature. After an upward shift, the topR1 expression pattern is inversely regulated with a transient decrease with the same time course. The topR1 expression profile is completely different from that of topR2 after temperature shift experiments; this suggests a different regulation process for the two reverse gyrase genes. The fine tuning of the topR1 transcript expression within a short interval of time after a temperature shift illustrates a rapid adaptation response to temperature change.
Garnier
Florence
F
Laboratoire de Génétique et Biologie Cellulaire, Université de Versailles-Saint-Quentin-en-Yvelines, CNRS UMR 8159, Bâtiment Buffon, 45 Avenue des Etats-Unis, 78035, Versailles cedex, France.
Nadal
Marc
M
D001483:Base Sequence; D015152:Blotting, Northern; D027081:DNA Gyrase / Q000235:genetics*; D017931:DNA Primers; D019766:Genes, Archaeal; D006358:Hot Temperature; D012333:RNA, Messenger / Q000235:genetics; D048229:Sulfolobus solfataricus / Q000235:genetics* / Q000254:growth & development*; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D027081:DNA Gyrase
Mol Genet Genomics. 2002 May;267(3):380-90
12073040
Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1853-8
11830640
Proc Natl Acad Sci U S A. 1993 May 15;90(10):4753-7
8389456
Biochem Biophys Res Commun. 2001 May 4;283(2):384-91
11327713
Annu Rev Biophys Biomol Struct. 2004;33:95-118
15139806
J Mol Biol. 1971 Feb 14;55(3):523-33
4927945
Genome Biol. 2004;5(11):R87
15535863
Biochimie. 2007 Apr;89(4):427-46
17293019
Exp Parasitol. 1999 Feb;91(2):126-32
9990340
Nucleic Acids Res. 2006 Apr 14;34(7):2098-108
16617150
Mol Microbiol. 1999 Aug;33(4):766-77
10447886
Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2939-44
17307872
Nature. 1984 Jun 21-27;309(5970):677-81
6328327
Mol Microbiol. 2003 Apr;48(1):143-56
12657051
Proc Natl Acad Sci U S A. 1972 Jan;69(1):143-6
4333036
Mol Microbiol. 1997 Mar;23(6):1267-79
9106217
Trends Microbiol. 1997 Aug;5(8):323-6
9263411
Genes Cells. 2001 Apr;6(4):303-12
11318873
Nat Rev Mol Cell Biol. 2002 Jun;3(6):430-40
12042765
Nucleic Acids Res. 1993 Feb 11;21(3):463-8
8441659
J Biol Chem. 1994 Feb 18;269(7):5255-63
8106509
J Bacteriol. 2004 Jul;186(14):4829-33
15231817
Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7835-40
11427726
Annu Rev Biochem. 2001;70:369-413
11395412
Cell. 1983 Aug;34(1):105-13
6309403
Biochim Biophys Acta. 1998 Oct 1;1400(1-3):19-27
9748482
Nucleic Acids Res. 1996 Dec 1;24(23):4668-75
8972852
Genome Biol. 2006;7(10):R99
17067383
Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15218-22
9860949
Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):106-10
8552584
Nucleic Acids Res. 1999 Oct 1;27(19):3821-35
10481021
J Biol Chem. 2000 Jun 30;275(26):19498-504
10748189
EMBO J. 2002 Feb 1;21(3):418-26
11823434
J Biol Chem. 2000 Mar 17;275(11):8103-13
10713132
Mol Biol Cell. 1998 Dec;9(12):3273-97
9843569
EMBO J. 1985 Aug;4(8):2123-8
14708549
Biochemistry. 1988 Dec 27;27(26):9102-8
2853975
Biochimie. 2007 Apr;89(4):447-55
17316953
Trends Genet. 2002 May;18(5):236-7
12047940