Expression of murine Asb-9 during mouse spermatogenesis.
We previously showed that Asb-4 and Asb-17 is uniquely expressed in developing male germ cells. A recent report showed that Asb-9 is specifically expressed in the kidney and testes; however, detailed expression patterns in developing germ cells have not been shown. Northern blot analysis in various tissues demonstrated that mAsb-9 was strongly expressed in the testes. Expression analysis by RT-PCR and Northern blot in developing mouse testes indicates that mAsb-9 is expressed from the fourth week after birth to adulthood, with the highest expression in round spermatids. Expression sites were further localized by in situ hybridization in the testes. Pachytene spermatocytes and spermatids expressed mAsb-9 but spermatogonia and generated spermatozoa did not. This study reveals that mAsb-9 could be a specific marker of active spermatogenesis and would be useful for studies of male germ cell development.
Lee
Man Ryul
MR
Department of Anatomy and Cell Biology, Hanyang University, College of Medicine, Seoul 133-791, Korea.
Kim
Soo Kyoung
SK
Kim
Jong Soo
JS
Rhim
Si Youn
SY
Kim
Kye-Seong
KS
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013091:Spermatogenesis / Q000235:genetics*; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis*; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution
C518251:Asb9 protein, mouse; D050826:Suppressor of Cytokine Signaling Proteins