Improvement of differentiation and mineralization of pre-osteoblasts on composite nanofibers of poly(lactic acid) and nanosized bovine bone powder.
The effect of NBM incorporation in PLA nanofibers on their mechanical properties and the differentiation and mineralization of osteoblasts was studied. At 20% NBM, the Young's modulus of the nanofibers was 37.78 +/- 4.23, significantly larger than that of pure PLA nanofibers. MC3T3-E1 pre-osteoblasts attached to both types of nanofibers and developed full osteogenic phenotypes. A profound effect of NBM on the mineralization of MC3T3-E1 pre-osteoblasts was confirmed, suggesting that NBM/PLA composite nanofibers exhibit properties similar to those of the native collagen-rich mineralized bone matrix, and could therefore serve as a temporary substrate for facilitating the differentiation and mineralization of bone-forming cells.
Ko
Eun Kyoung
EK
Department of Bioengineering, Hanyang University, Seongdong-gu, Seoul, Republic of Korea.
Jeong
Sung In
SI
Lee
Ji Hye
JH
Shin
Heungsoo
H
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D001842:Bone and Bones / Q000737:chemistry*; D002113:Calcification, Physiologic; D002417:Cattle; D017931:DNA Primers; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D010006:Osteoblasts / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011208:Powders; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering
D017931:DNA Primers; D011091:Polyesters; D011108:Polymers; D011208:Powders; D019344:Lactic Acid; C033616:poly(lactide)