title: Surface treatment of pure and PEG-4000 blended fibroin films and their characterizations as matrices for in vitro fibroblast culture.
abstract: This study reports the effects of treatment with various concentrations of organic solvents for varying time points on matrices of fibroin, a silk protein isolated from the mulberry silkworm, Bombyx mori, which in native form has been extensively used in tissue engineering. Treatment of pure fibroin as well as polyethylene glycol- blended films with 90% organic solvent for 60 min induces optimal surface hydrophobicity and maximum conversion of the secondary structure from random coil to beta sheet. Long-term cell viability studies reveal that methanol and isopropanol-treated pure and blended films support cell adhesion, proliferation, and viability.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur-721302, India.
authors.forename: Chitrangada
authors.initials: C
authors.lastname: Acharya
authors.forename: Anindya
authors.initials: A
authors.lastname: Dutta
authors.forename: S C
authors.initials: SC
authors.lastname: Kundu
mesh_terms: D019840:2-Propanol / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology*; D005349:Fibroins / Q000737:chemistry*; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry*; D047011:Silk / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D011092:Polyethylene Glycols; D005349:Fibroins; D019840:2-Propanol