Candida albicans-secreted lipase induces injury and steatosis in immune and parenchymal cells.
Virulence depends on opposing reactions between host and pathogen and is intrinsically linked to the host immune status. Virulence factors rely upon microbial attributes that mediate cell damage. While the activity of several Candida albicans hydrolytic enzymes is well characterized, the biological role of lipases is uncertain. In this report, we identified, isolated, and characterized a C. albicans 70 kDa lipase that exhibited maximal activity at physiological pH and temperature. We evaluated the ability of C. albicans lipase to interact with two types of mammalian host cells: macrophages, as crucial immune effector cells involved in fungal control, and hepatocytes, as examples of parenchymal cells compromised during fungal dissemination. Herein, we demonstrate for the first time that an extracellular lipase released by C. albicans directly induced cytotoxicity and promoted the deposition of lipid droplets in the cytoplasm of macrophages and hepatocytes.
Paraje
María Gabriela
MG
Immunology, Department of Clinical Biochemistry, CIBICI-CONICET, Faculty of Chemical Science, National University of Cordoba, Córdoba 5000, Argentina.
Correa
Silvia Graciela
SG
Renna
María Sol
MS
Theumer
Martín
M
Sotomayor
Claudia Elena
CE
D000818:Animals; D002176:Candida albicans / Q000201:enzymology* / Q000276:immunology; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005234:Fatty Liver / Q000276:immunology* / Q000382:microbiology; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology*; D022781:Hepatocytes / Q000276:immunology / Q000382:microbiology; D006801:Humans; D008049:Lipase / Q000235:genetics / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051381:Rats; D017208:Rats, Wistar
D005656:Fungal Proteins; D008049:Lipase