title: Determination of bacterial biomass from flow cytometric measurements of forward light scatter intensity.
abstract: This unit presents methods for measuring biomass of bacteria by flow cytometry. Such measurements are an integral part of determining growth rates and kinetics. This unit discusses calibration and standardization methods for bacterial studies using flow cytometry and provides a detailed discussion of the components involved. The serious microbiologist will appreciate the advantages flow cytometry brings to many aspects of microbial study, and this unit is no exception.
authors.affiliation: University of Alaska Fairbanks, Fairbanks, Alaska, USA.
authors.forename: B R
authors.initials: BR
authors.lastname: Robertson
authors.forename: D K
authors.initials: DK
authors.lastname: Button
mesh_terms: D000465:Algorithms; D001419:Bacteria / Q000378:metabolism*; D018533:Biomass; D002138:Calibration; D004926:Escherichia coli / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D007700:Kinetics; D008027:Light; D012542:Scattering, Radiation