title: Genetic Manipulation of Mycobacterium tuberculosis.
abstract: This unit includes protocols for the genetic manipulation of Mycobacterium tuberculosis, including nucleic acid extraction (plasmid DNA, genomic DNA, and mRNA), and methods for electroporation (transformation), transduction (including allelic exchange), and transposon mutagenesis. Considerations for working with M. tuberculosis at Biosafety Level 3 containment are also discussed.
authors.affiliation: Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Michelle H
authors.initials: MH
authors.lastname: Larsen
authors.forename: Karolin
authors.initials: K
authors.lastname: Biermann
authors.forename: Steven
authors.initials: S
authors.lastname: Tandberg
authors.forename: Tsugunda
authors.initials: T
authors.lastname: Hsu
authors.forename: William R
authors.initials: WR
authors.lastname: Jacobs
mesh_terms: D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D018274:Electroporation; D005821:Genetic Techniques; D006801:Humans; D016296:Mutagenesis; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D014161:Transduction, Genetic
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial