Morphometric and histological analysis of low-power laser influence on bone morphogenetic protein in bone defects repair.
Bone morphogenetic proteins (BMPs) are secreted signaling molecules belonging to the transforming growth factor-beta (TGF-beta) superfamily. The objective of this study was to determine how gallium-aluminum-arsenium (GaAlAs) 650 nm laser influenced the action of BMPs on bone defects created in rat femurs. The sample consisted of 24 male albino Wistar rats. Group 1 was composed of rats with bone defects filled with bone-inducing substance, with the application of low-power laser. Group 2 contained rats with bone defects filled with a bone-inducing substance, without the application of low-power laser. Group 3 rats had bone defects not filled with a bone-inducing substance, with the application of low-power laser. Group 4 rats had bone defects and no treatment (control group). A bone defect was produced with drills. In groups 1 and 2 the defects were filled with a bone-inducing substance. The animals were treated with GaAlAs (50 mW) laser, energy density 4 J/cm(2), for 80 ss on a 1 cm(2) area. Groups 2 and 4 were used as control. Bone samples were removed for histological procedures and morphometric analysis on the 7th, 14th and 21st days after surgery. Results obtained were subjected to statistical analysis. Rejection level for the null hypothesis was 0.05. Statistical differences were found in the comparison between group 1 (G1), G2, G3 and G4 [analysis of variance (ANOVA); P < 0.0134]. There was a statistically significant correlation between groups 1 and 4 (P < 0.01). The results of other correlations by Tukey's post-hoc test were: group 1 vs group 3 (P = 0.341), group 1 vs group 2 (P = 0.862), group 2 vs group 4 (P = 0.061), group 2 vs group 3 (P = 0.744), and group 3 vs group 4 (P = 0.249). We concluded that the association of low-power laser with a bone-inducing substance produced better results than when low-power laser or BMPs were used alone.
Denadai
Amanda Silveira
AS
Postgraduate Program on Health and Development in West Central Region, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
de Carvalho
Paulo de Tarso Camillo
Pde T
dos Reis
Filipe Abdalla
FA
Belchior
Ana Carulina Guimarães
AC
Pereira
Daniel Martins
DM
Dourado
Doroty Mesquita
DM
Silva
Iandara S
IS
de Oliveira
Luis Vicente Franco
LV
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology / Q000502:physiology*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D018786:Bone Substitutes; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology / Q000502:physiology / Q000528:radiation effects*; D028022:Low-Level Light Therapy; D008297:Male; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar
D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes
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