[Study on the determination of bovine serum albumin using o-hydroxyphenylfluorone reagent with fluorescence spectrophotometry].
The reactions were studied for the determination of bovine serum albumin (BSA) using o-hydroxyphenylfluorone (o-HPF) as fluorescence probe reagent with fluorescence spectrophotometry. In the B-R medium, o-HPF reacts with BSA to form the stable complex compound. The reaction system of o-HPF and BSA was binary system, and the method for the determination of BSA was of good selectivity and stability and simplicity. The linear range of o-HPF fluorescence spectrophotometric method was 1.32-18.54 microg x mL(-1) The quenching constant and thermodynamics constant of the fluorescence effects between BSA and o-HPF were calculated. The results showed that the non-covalent binding forces were the binding force between o-HPF and BSA.
Huang
Ying-ping
YP
China Three Gorges University Alan G. MacDiarmid Institute for Renewable Energy, Yichang 443002, China. huangyp@ctgu.edu.cn
Gu
Yan
Y
Fang
Yan-fen
YF
Liu
Yu-liang
YL
Zhu
Sheng-ji
SJ
Lou
Guang-fu
GF
D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature; D013816:Thermodynamics
D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine