Candida albicans and Candida tropicalis in oral candidosis: quantitative analysis, exoenzyme activity, and antifungal drug sensitivity.
Candida albicans and C. tropicalis obtained from whole saliva of patients presenting signs of oral candidosis were assayed for quantification of colony forming units, exoenzyme activity (phospholipase and proteinase) and antifungal drug sensitivity (amphotericin B, fluconazole and itraconazole) by the reference method of the Clinical and Laboratory Standards Institute. The number of colony forming units per milliliter varied according to the Candida species involved and whether a single or mixed infection was present. Proteinase activity was observed in both C. albicans and C. tropicalis, but phospholipase activity was noted only in C. albicans. In vitro resistance to antifungals was verified in both species, but C. tropicalis appears to be more resistant to the tested antifungals than C. albicans.
da Costa
Karen Regina Carim
KR
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, 14040-903, Ribeirão Preto, SP, Brazil. krccosta@fcfrp.usp.br
Ferreira
Joseane Cristina
JC
Komesu
Marilena Chinali
MC
Candido
Regina Celia
RC
D000328:Adult; D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D041022:Candida tropicalis / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000382:microbiology*; D015169:Colony Count, Microbial; D025141:Drug Resistance, Fungal; D005260:Female; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D010740:Phospholipases / Q000378:metabolism
D000935:Antifungal Agents; D017964:Itraconazole; D000666:Amphotericin B; D015725:Fluconazole; D010740:Phospholipases; D016282:Aspartic Acid Endopeptidases
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