Design and chemical synthesis of a 2',4'-BNA probe for 7,8-dihydro-8-oxoguanine-containing double stranded DNA.
7,8-Dihydro-8-oxoguanine (8-OxoG), caused by DNA oxidation, is considered to be a marker of oxidative stress, and also to be a cause of genomic diversity in organism because of its mutagenic potential. Here, we designed and synthesized a 2',4'-BNA probe for 8-OxoG-containing double stranded DNA (dsDNA). The designed probe showed an 8-OxoG:C base pair selective triplex-forming ability.
Miyoshi
Tetsuya
T
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Kodama
Tetsuya
T
Obika
Satoshi
S
Imanishi
Takeshi
T
D004247:DNA / Q000737:chemistry*; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry
C453560:7,8-dihydro-8-oxoguanine; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; C051802:triplex DNA; D006147:Guanine; D004247:DNA