Expression of lipid storage droplet protein-1 may define the role of AKH as a lipid mobilizing hormone in Manduca sexta.
Adipokinetic hormone (AKH) is the main hormone involved in the acute regulation of hemolymph lipid levels in several insects. In adult Manduca sexta AKH promotes a rapid phosphorylation of "Lipid storage protein-1", Lsd1, and a concomitant activation of the rate of hydrolysis of triglycerides by the main fat body lipase. In contrast, in the larval stage AKH modulates hemolymph trehalose levels. The present study describes the sequence of a full-length Lsd1 cDNA obtained from M. sexta fat body and investigates a possible link between Lsd1 expression and the distinct effects of AKH in larva and adult insects. The deduced protein sequence showed a high degree of conservation compared to other insect Lsd1s, particularly in the central region of the protein (amino acids 211-276) in which the predicted lipid binding helices are found. Lsd1 was absent in feeding larva and its abundance progressively increased as the insect develops from the non-feeding larva to adult. Contrasting with the levels of protein, Lsd1 transcripts were maximal during the feeding larval stages. The subcellular distribution of Lsd1 showed that the protein exclusively localizes in the lipid droplets. Lsd1 was found in the fat body but it was undetectable in lipid droplets isolated from oocytes or embryos. The present study suggests a link between AKH-stimulated lipolysis in the fat body and the expression of Lsd1.
Arrese
Estela L
EL
Department of Biochemistry and Molecular Biology, Oklahoma State University, 246 Noble Research Center, Stillwater, OK 74078, USA. destela@okstate.edu
Mirza
Saima
S
Rivera
Laticia
L
Howard
Alisha D
AD
Chetty
Palaniappan S
PS
Soulages
Jose L
JL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry; D005216:Fat Body / Q000378:metabolism; D007301:Insect Hormones / Q000494:pharmacology / Q000502:physiology*; D019476:Insect Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism; D018402:Manduca / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology / Q000502:physiology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D016415:Sequence Alignment
D018076:DNA, Complementary; D007301:Insect Hormones; D019476:Insect Proteins; D009842:Oligopeptides; C044833:adipokinetic hormone; D011761:Pyrrolidonecarboxylic Acid
Int Rev Cytol. 1998;182:249-302
9522462
Mech Dev. 2003 Sep;120(9):1071-81
14550535
J Biol Chem. 2005 Jun 17;280(24):22624-31
15829485
Arch Biochem Biophys. 2008 May 1;473(1):42-7
18342616
Tissue Cell. 1988;20(4):635-43
3238692
Mol Cell Biol. 1989 Nov;9(11):5073-80
2601709
Insect Mol Biol. 1999 Feb;8(1):39-53
9927173
Mol Cell Biol. 1989 Nov;9(11):5134-42
2601712
Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002
2461560
Insect Biochem Mol Biol. 2001 Jan;31(1):7-17
11102830
Insect Biochem Mol Biol. 2008 Jun;38(6):677-82
18510979
Peptides. 1990 Sep-Oct;11(5):1037-40
2284195
Prog Lipid Res. 2001 Sep;40(5):325-438
11470496
Insect Mol Biol. 1996 Feb;5(1):31-8
8630533
J Lipid Res. 2006 Dec;47(12):2656-67
17005997
J Biol Chem. 2004 Jan 30;279(5):3787-92
14597625
Proteomics. 2004 Jun;4(6):1633-49
15174133
J Biol Chem. 2002 Aug 30;277(35):32253-7
12077142
J Comp Physiol B. 1991;161(2):125-31
1869693
J Lipid Res. 2007 Dec;48(12):2547-59
17878492
Arch Insect Biochem Physiol. 1999 Nov;42(3):198-212
10536048
Gene. 2002 Jul 10;294(1-2):147-56
12234676
Nucleic Acids Res. 1987 Feb 25;15(4):1353-61
3822832
Mamm Genome. 2001 Sep;12(9):741-9
11641724
Gen Comp Endocrinol. 2003 Jun 1;132(1):10-20
12765639
Insect Biochem Mol Biol. 2004 Dec;34(12):1269-79
15544940
J Lipid Res. 1999 Mar;40(3):556-64
10064744
J Lipid Res. 1997 Jan;38(1):68-76
9034201
Biochimie. 1994;76(9):880-6
7880905
Methods Enzymol. 1996;266:540-53
8743705
Arch Insect Biochem Physiol. 2006 Oct;63(2):73-81
16983668
Genomics. 2005 Mar;85(3):360-71
15718103
Mol Cell Proteomics. 2006 Jun;5(6):1082-94
16543254