title: Susceptibility of peripheral lymphocytes of brain tumour patients to in vitro radiation-induced DNA damage, a preliminary study.
abstract: OBJECTIVE
  The present investigation aimed to study the susceptibility of lymphocytes collected from brain tumour patients to radiation-induced DNA damage under in vitro conditions.
  
  
  METHODS
  The peripheral lymphocytes collected from brain tumour patients were exposed to 2-Gy gamma radiation. Susceptibility of lymphocytes to radiation-induced DNA damage and their repair ability was assessed by alkaline comet assay.
  
  
  RESULTS
  Lymphocytes of patients with benign and malignant tumour had a significantly higher (p < 0.001) baseline DNA damage compared to lymphocytes from normal subjects. A significant increase (p < 0.001) in DNA damage was observed immediately after irradiation of lymphocytes from healthy subjects and brain tumour patients. However, at 1 h after irradiation the level of DNA damage dropped significantly (p < 0.001) compared to that of immediately after irradiation of respective groups.
  
  
  CONCLUSIONS
  In conclusion, the lymphocytes of brain tumour patients possess a higher level of basal DNA damage and exhibit a higher susceptibility to a clastogenic agent like radiation.
authors.affiliation: Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Medical College and Hospital, Manipal, Karnataka, India.
authors.forename: Guruprasad
authors.initials: G
authors.lastname: Kalthur
authors.forename: Prem
authors.initials: P
authors.lastname: Kumar
authors.forename: Uma
authors.initials: U
authors.lastname: Devi
authors.forename: Sabir
authors.initials: S
authors.lastname: Ali
authors.forename: Ramya
authors.initials: R
authors.lastname: Upadhya
authors.forename: Sailaja
authors.initials: S
authors.lastname: Pillai
authors.forename: Anjali
authors.initials: A
authors.lastname: Rao
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000097:blood* / Q000532:radiotherapy; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male
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