Measles viruses possessing the polymerase protein genes of the Edmonston vaccine strain exhibit attenuated gene expression and growth in cultured cells and SLAM knock-in mice.
Live attenuated vaccines against measles have been developed through adaptation of clinical isolates of measles virus (MV) in various cultured cells. Analyses using recombinant MVs with chimeric genomes between wild-type and Edmonston vaccine strains indicated that viruses possessing the polymerase protein genes of the Edmonston strain exhibited attenuated viral gene expression and growth in cultured cells as well as in mice expressing an MV receptor, signaling lymphocyte activation molecule, regardless of whether the virus genome had the wild-type or vaccine-type promoter sequence. These data demonstrate that the polymerase protein genes of the Edmonston strain contribute to its attenuated phenotype.
Takeda
Makoto
M
Department of Virology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. mtakeda@virology.med.kyushu-u.ac.jp
Ohno
Shinji
S
Tahara
Maino
M
Takeuchi
Hiroki
H
Shirogane
Yuta
Y
Ohmura
Hirofumi
H
Nakamura
Takafumi
T
Yanagi
Yusuke
Y
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000502:physiology*; D002522:Chlorocebus aethiops; D049452:Green Fluorescent Proteins / Q000235:genetics; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000235:genetics*; D051379:Mice; D012367:RNA, Viral / Q000096:biosynthesis; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics* / Q000276:immunology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014613:Vaccines, Attenuated / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D014709:Vero Cells
D015703:Antigens, CD; D008458:Measles Vaccine; D012367:RNA, Viral; D011956:Receptors, Cell Surface; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D012324:RNA-Dependent RNA Polymerase
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