title: Genetic manipulation of Leptospira biflexa.
abstract: The genus Leptospira belongs to the order Spirochaetales and is composed of both saprophytic and pathogenic members, such as Leptospira biflexa and L. interrogans, respectively. A major factor contributing to our ignorance of spirochetal biology has been the lack of methods available for genetic analysis of these organisms. In recent years, an E. coli-L. biflexa shuttle vector has been constructed and a system for targeted mutagenesis and random transposon mutagenesis of the saprophyte L. biflexa has been developed. These studies enable the use of L. biflexa as a model bacterium among spirochetes.
authors.affiliation: Unité de Biologie des Spirochètes Institut Pasteur, Paris, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Louvel
authors.forename: Mathieu
authors.initials: M
authors.lastname: Picardeau
mesh_terms: D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D005822:Genetic Vectors; D006801:Humans; D007919:Leptospira / Q000145:classification / Q000235:genetics*; D016296:Mutagenesis; D014169:Transformation, Bacterial
chemical_list: D004251:DNA Transposable Elements