Protective effects of epigallocatechin gallate after UV irradiation of cultured human lens epithelial cells.
OBJECTIVE
To evaluate the protective effects of epigallocatechin gallate (EGCG) against UV irradiation of cultured human lens epithelial cells.


METHODS
We irradiated cultured human lens epithelial cells with a 30-second pulse from a UV lamp with an irradiance of 0.6 mW/cm2. Five minutes and 1 hour after UV irradiation, we administered 0, 5, 10, 15, 25, 50, or 100 uM EGCG. The cell number was measured with a microscopic counting chamber and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.


RESULTS
Compared to untreated cells, the total number of cultured human lens epithelial cells was markedly higher after UV irradiation. In a dose-dependent manner, viability was also higher in EGCG-treated cells.


CONCLUSIONS
EGCG increased the cell count and cell viability after UV irradiation of cultured human lens epithelial cells, indicating that EGCG can protect lens epithelium against UV damage.
Heo
Jun
J
Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Korea.
Lee
Byung Rae
BR
Koh
Jae Woong
JW
D000975:Antioxidants / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000528:radiation effects; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000528:radiation effects*; D011832:Radiation Injuries / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014466:Ultraviolet Rays
D000975:Antioxidants; D004396:Coloring Agents; D011837:Radiation-Protective Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D002392:Catechin; C045651:epigallocatechin gallate; C022616:thiazolyl blue
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