title: Expression of anaphase-promoting complex7 in fibroadenomas and phyllodes tumors of breast.
abstract: The proteolytic destruction of cyclin B is an important event during cell division. Cyclin B proteolysis is triggered by the anaphase-promoting complex. Therefore, cell cycle dysregulation due to anaphase-promoting complex loss contributes to cell transformation and human carcinogenesis. This study investigates anaphase-promoting complex7 expression in spindle cell breast tumors and also includes a comparison between the proliferation antigen Ki-67 and S-phase fraction. The average values of the anaphase-promoting complex7 and Ki-67 labeling indices increased in order from benign to malignant within the phyllodes tumor group, and the fibroadenoma and juvenile fibroadenoma exhibited lower levels of anaphase-promoting complex7 and Ki-67 expression than did the phyllodes tumor. The frequency of anaphase-promoting complex7-positive stromal cells correlated with Ki-67 expression in phyllodes tumor and in all of the examined breast tumors. The above results indicate that anaphase-promoting complex7 and Ki-67 are closely related to cell proliferation. In addition, phyllodes tumor can be differentiated from juvenile fibroadenoma with increased mitotic figures mimicking phyllodes tumor by anaphase-promoting complex7 and Ki-67 immunochemistry. Because anaphase-promoting complex7 is expressed at higher levels than is Ki-67, it may overcome the limitations of the Ki-67 labeling index with regard to the differentiation of benign phyllodes tumor from fibroadenoma.
authors.affiliation: Institute for Medical Science, Ajou University School of Medicine, Suwon 443-721, Korea.
authors.forename: Yup
authors.initials: Y
authors.lastname: Kang
authors.forename: Jang Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Tae Hui
authors.initials: TH
authors.lastname: Lee
authors.forename: Tai Seung
authors.initials: TS
authors.lastname: Kim
authors.forename: Woo Hee
authors.initials: WH
authors.lastname: Jung
authors.forename: Hyun Cheol
authors.initials: HC
authors.lastname: Chung
authors.forename: Byeong Woo
authors.initials: BW
authors.lastname: Park
authors.forename: Seung Soo
authors.initials: SS
authors.lastname: Sheen
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Han
mesh_terms: D000293:Adolescent; D000328:Adult; D064173:Anaphase-Promoting Complex-Cyclosome; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D018226:Fibroadenoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008875:Middle Aged; D003557:Phyllodes Tumor / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012189:Retrospective Studies; D016196:S Phase; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D019394:Ki-67 Antigen; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome