High-sensitivity simultaneous analysis of ubiquinol-10 and ubiquinone-10 in human plasma.
A method to determine ubiquinol-10 and ubiquinone-10 in human serum was developed by using high-performance liquid chromatography consisting of a semi-microcolumn switching system and an electrochemical detector (ECD), which requires minimized sample pre-treatments. A linear dynamic range was obtained from 1.0 to 5000 ng/mL, and recovery values of 89-105% were observed in a low-concentration region of 10-50 ng/mL. In a long operation test, a good precision was maintained during 5100 runs without any maintenance on ECD or columns. In addition, retention behaviors of other ubiquinone homologues were examined.
Hirayama
Aya
A
Shiseido Research Center, 2-2-1 Hayabuchi, Yokohama 224-8558, Japan.
Kubo
Hiroshi
H
Mita
Masashi
M
Shirota
Osamu
O
Yamamoto
Yorihiro
Y
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood
D014451:Ubiquinone; C025204:Ubiquinone Q2; C003741:ubiquinol