title: Mesenchymal stem cells from the Wharton's jelly of umbilical cord segments provide stromal support for the maintenance of cord blood hematopoietic stem cells during long-term ex vivo culture.
abstract: BACKGROUND
  Hematopoietic stem cells (HSCs) are routinely obtained from marrow, mobilized peripheral blood, and umbilical cord blood. Mesenchymal stem cells (MSCs) are traditionally isolated from marrow. Bone marrow-derived MSCs (BM-MSCs) have previously demonstrated their ability to act as a feeder layer in support of ex vivo cord blood expansion. However, the use of BM-MSCs to support the growth, differentiation, and engraftment of cord blood may not be ideal for transplant purposes. Therefore, the potential of MSCs from a novel source, the Wharton's jelly of umbilical cords, to act as stromal support for the long-term culture of cord blood HSC was evaluated.
  
  
  METHODS
  Umbilical cord-derived MSCs (UC-MSCs) were cultured from the Wharton's jelly of umbilical cord segments. The UC-MSCs were then profiled for expression of 12 cell surface receptors and tested for their ability to support cord blood HSCs in a long-term culture-initiating cell (LTC-IC) assay.
  
  
  RESULTS
  Upon culture, UC-MSCs express a defined set of cell surface markers (CD29, CD44, CD73, CD90, CD105, CD166, and HLA-A) and lack other markers (CD45, CD34, CD38, CD117, and HLA-DR) similar to BM-MSCs. Like BM-MSCs, UC-MSCs effectively support the growth of CD34+ cord blood cells in LTC-IC assays.
  
  
  CONCLUSIONS
  These data suggest the potential therapeutic application of Wharton's jelly-derived UC-MSCs to provide stromal support structure for the long-term culture of cord blood HSCs as well as the possibility of cotransplantation of genetically identical, HLA-matched, or unmatched cord blood HSCs and UC-MSCs in the setting of HSC transplantation.
authors.affiliation: Division of Maternal-Fetal Medicine, The University of Texas Medical School at Houston, Houston, Texas, USA.
authors.forename: Tiki
authors.initials: T
authors.lastname: Bakhshi
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Zabriskie
authors.forename: Shamanique
authors.initials: S
authors.lastname: Bodie
authors.forename: Shannon
authors.initials: S
authors.lastname: Kidd
authors.forename: Susan
authors.initials: S
authors.lastname: Ramin
authors.forename: Laura A
authors.initials: LA
authors.lastname: Paganessi
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Gregory
authors.forename: Henry C
authors.initials: HC
authors.lastname: Fung
authors.forename: Kent W
authors.initials: KW
authors.lastname: Christopherson
mesh_terms: D002469:Cell Separation / Q000379:methods*; D048430:Cell Shape; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013997:Time Factors
references.citation: J Virol. 2002 Jul;76(13):6636-42
references.pmid: 12050376
references.citation: Bone Marrow Transplant. 2006 Feb;37(4):359-66
references.pmid: 16400333
references.citation: Stem Cells. 2005 Feb;23(2):220-9
references.pmid: 15671145
references.citation: Stem Cells. 2003;21(1):50-60
references.pmid: 12529551
references.citation: J Immunol. 2002 Dec 15;169(12):7000-8
references.pmid: 12471135
references.citation: Braz J Med Biol Res. 2003 Sep;36(9):1179-83
references.pmid: 12937783
references.citation: Cytotherapy. 2006;8(4):315-7
references.pmid: 16923606
references.citation: Br J Haematol. 2003 Apr;121(2):368-74
references.pmid: 12694261
references.citation: Stem Cells. 2006 Mar;24(3):781-92
references.pmid: 16223852
references.citation: Stem Cells. 2004;22(7):1330-7
references.pmid: 15579650
references.citation: N Engl J Med. 1989 Oct 26;321(17):1174-8
references.pmid: 2571931
references.citation: Blood Cells. 1994;20(2-3):560-4; discussion 564-5
references.pmid: 7749121
references.citation: Stem Cells. 2004;22(5):649-58
references.pmid: 15342929
references.citation: Biochem Soc Trans. 1991 Feb;19(1):29S
references.pmid: 1709890
references.citation: Biol Blood Marrow Transplant. 2007 Dec;13(12):1477-86
references.pmid: 18022578
references.citation: Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4698-703
references.pmid: 9114054
references.citation: Methods Mol Med. 2002;63:123-41
references.pmid: 21437804
references.citation: Blood Cells. 1994;20(2-3):227-33; discussion 233-4
references.pmid: 7749099
references.citation: Blood. 1998 Jan 1;91(1):111-7
references.pmid: 9414274
references.citation: Int J Mol Med. 2006 Dec;18(6):1089-96
references.pmid: 17089012
references.citation: Chin Med J (Engl). 2005 Dec 5;118(23):1987-93
references.pmid: 16336835
references.citation: Proc Natl Acad Sci U S A. 1989 May;86(10):3828-32
references.pmid: 2566997
references.citation: Exp Hematol. 2002 Aug;30(8):870-8
references.pmid: 12160838
references.citation: Proc Natl Acad Sci U S A. 1990 May;87(9):3584-8
references.pmid: 2333304
references.citation: Stem Cells. 2003;21(1):105-10
references.pmid: 12529557
references.citation: Cytotherapy. 2004;6(4):311-7
references.pmid: 16146883