Thermodynamic studies of interactions of calf spleen PNP with acyclic phosphonate inhibitors.
The Gibbs binding energy and entropy/enthalpy contributions to the interaction of calf spleen purine nucleoside phosphorylase (PNP) with the novel multisubstrate analogue DFPP-DG, as well as with DFPP-G and (S)-PMP-DAP were determined by fluorescence and calorimetric studies. Results were compared with findings for guanine - a natural reaction product and inhibitor.
Breer
Katarzyna
K
Department of Biophysics, Institute of Experimental Physics, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland.
Wielgus-Kutrowska
Beata
B
Hashimoto
Mariko
M
Hikishima
Sadao
S
Yokomatsu
Tsutomu
T
Szczepanowski
Roman H
RH
Bochtler
Matthias
M
Girstun
Agnieszka
A
Starón
Krzysztof
K
Bzowska
Agnieszka
A
D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry*; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D063065:Organophosphonates / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D011683:Purine-Nucleoside Phosphorylase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D013154:Spleen / Q000201:enzymology; D013816:Thermodynamics
C090125:9-(2-phosphonylmethoxypropyl)-2,6-diaminopurine; C528379:9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine; C509941:9-(5',5'-difluoro-5'-phosphonopentyl)guanine; D004791:Enzyme Inhibitors; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D006147:Guanine; D011683:Purine-Nucleoside Phosphorylase; D000225:Adenine