A simple high-performance liquid chromatographic method for the estimation of boswellic acids from the market formulations containing Boswellia serrata extract.
A simple, rapid, and reproducible reverse-phase high-performance liquid chromatographic method is developed for the estimation of boswellic acids, the active constituents in Boswellia serrata oleo-gum resin. The chromatographic separation is performed using a mobile phase consisting of acetonitrile-water (90:10, % v/v) adjusted to pH 4 with glacial acetic acid on a Kromasil 100 C18 analytical column with flow rate of 2.0 mL/min and detection at 260 nm. The elution times are 4.30 and 7.11 min for 11-keto beta-boswellic acid (11-KBA) and 3-acetyl 11-keto beta-boswellic acid (A-11-KBA), respectively. The calibration curve is linear in the 11.66-58.30 microg/mL and 6.50-32.50 microg/mL range for 11-KBA and A-11-KBA, respectively. The limits of detection are 2.33 microg/mL and 1.30 microg/mL for 11-KBA and A-11-KBA, respectively. The mean recoveries are 98.24% to 104.17% and 94.12% to 105.92% for 11-KBA and A-11-KBA, respectively. The inter- and intra-day variation coefficients are less than 5%. The present method is successfully applied for the estimation of boswellic acids from the market formulations containing Boswellia serrata extract.
Shah
Shailesh A
SA
Department of Quality Assurance, L.M. College of Pharmacy, Navrangpura, Ahmedabad-380 009, India. shaileshah_lmcp@rediffmail.com
Rathod
Ishwarsinh S
IS
Suhagia
Bhanubhai N
BN
Pandya
Saurabh S
SS
Parmar
Vijay K
VK
D029743:Boswellia / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014315:Triterpenes / Q000032:analysis*
D010936:Plant Extracts; D014315:Triterpenes; C054625:boswellic acid