title: Metal accumulation and oxidative stress responses in, cultured and wild, white seabream from Northwest Atlantic.
abstract: Metals are environmentally ubiquitous and can be found at high concentrations in seawater and subsequently in marine organisms. Metals with high redox potential can trigger oxidative stress mechanisms with damaging effects in biological tissues. In aquatic species, oxidative stress has been evaluated by assessing antioxidant enzymes activities and oxidative damages in tissues. The purpose of this study was to evaluate oxidative stress biomarkers and metal residues in white seabream (Diplodus sargus), a species entering aquaculture production in Portugal. Metal residues (Cu, Cd, As and Pb), in liver and muscle, as well as oxidative stress biomarkers were assessed at different stages in the life cycle of white seabream under culture conditions and in wild specimens, of a marketable size. Metal concentrations in tissues were low, and below the established limits. However, wild white seabream showed higher accumulation than cultured ones. Antioxidant enzymes, namely catalase (CAT) and superoxide dismutase (SOD), were correlated with metal accumulation. Oxidative damages to tissues were low, with wild white seabream showing lower levels than cultured fish. This study showed that white seabream has a good antioxidant defense system, capable of reducing oxidative damages in tissues resulting from the presence of metals.
authors.affiliation: CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental - Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal. mferreira@ciimar.up.pt
authors.forename: Marta
authors.initials: M
authors.lastname: Ferreira
authors.forename: Miguel
authors.initials: M
authors.lastname: Caetano
authors.forename: Joana
authors.initials: J
authors.lastname: Costa
authors.forename: Pedro
authors.initials: P
authors.lastname: Pousão-Ferreira
authors.forename: Carlos
authors.initials: C
authors.lastname: Vale
authors.forename: Maria Armanda
authors.initials: MA
authors.lastname: Reis-Henriques
mesh_terms: D000818:Animals; D001267:Atlantic Ocean; D002374:Catalase / Q000378:metabolism; D005398:Fisheries; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D019216:Metals, Heavy / Q000493:pharmacokinetics / Q000633:toxicity; D009132:Muscles / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D021541:Sea Bream / Q000254:growth & development / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D002374:Catalase; D013482:Superoxide Dismutase