Lack of correlation between MYCN expression and the Warburg effect in neuroblastoma cell lines.
BACKGROUND
Many cancers preferentially meet their energy requirements through the glycolytic pathway rather than via the more efficient oxidative phosphorylation pathway. It is thought that this is an important adaptation in cancer malignancy. We investigated whether use of glycolysis for energy production even in the presence of oxygen (known as the Warburg effect) varied between neuroblastoma cell lines with or without MYCN amplification (a key indicator of poor disease outcome in neuroblastoma).


METHODS
We examined ATP and lactate production, oxygen consumption and mitochondrial energisation status for three neuroblastoma cell lines with varying degrees of MYCN amplification and MYCN expression.


RESULTS
We found no correlation between MYCN expression and the Warburg effect in the cell lines investigated.


CONCLUSIONS
Our results suggest preferential use of glycolysis for energy production and MYCN expression may be independent markers of neuroblastoma malignancy in vitro if not in vivo.
Smith
Danielle J
DJ
Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Wellington Road, CLAYTON, Victoria 3800, Australia. danielle.smith@med.monash.edu.au
Cossins
Luke R
LR
Hatzinisiriou
Irene
I
Haber
Michelle
M
Nagley
Phillip
P
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D005784:Gene Amplification; D016259:Genes, myc / Q000235:genetics; D006019:Glycolysis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D019344:Lactic Acid / Q000096:biosynthesis*; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D000071447:N-Myc Proto-Oncogene Protein; D009363:Neoplasm Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology; D010641:Phenotype
C459801:MYCN protein, human; D000071447:N-Myc Proto-Oncogene Protein; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D015513:Oncogene Proteins; D019344:Lactic Acid; D000255:Adenosine Triphosphate
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