title: Fluorescence fluctuations analysis in nanoapertures: physical concepts and biological applications.
abstract: During the past years, nanophotonics has provided new approaches to study the biological processes below the optical diffraction limit. How single molecules diffuse, bind and assemble can be studied now at the nanometric level, not only in solutions but also in complex and crowded environments such as in live cells. In this context fluorescence fluctuations spectroscopy is a unique tool since it has proven to be easy to use in combination with nanostructures, which are able to confine light in nanometric volumes. We review here recent advances in fluorescence fluctuations' analysis below the optical diffraction limit with a special focus on nanoapertures milled in metallic films. We discuss applications in the field of single-molecule detection, DNA sequencing and membrane organization, and underscore some potential perspectives of this new emerging technology.
authors.affiliation: Institut Fresnel, UMR 6133 CNRS, Aix-Marseille University and Ecole Centrale Marseille, Domaine Universitaire de Saint Jérôme, 13397, Marseille Cedex 20, France. lenne@fresnel.fr
authors.forename: Pierre-François
authors.initials: PF
authors.lastname: Lenne
authors.forename: Hervé
authors.initials: H
authors.lastname: Rigneault
authors.forename: Didier
authors.initials: D
authors.lastname: Marguet
authors.forename: Jérôme
authors.initials: J
authors.lastname: Wenger
mesh_terms: D000818:Animals; D004798:Enzymes / Q000032:analysis; D004867:Equipment Design; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D015336:Molecular Probe Techniques / Q000295:instrumentation; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D017422:Sequence Analysis, DNA; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
chemical_list: D004798:Enzymes; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D008565:Membrane Proteins
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