title: Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns.
abstract: Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT)(4), (GT)(7) and (TA)(10) onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed along the chromosomes, and for each particular repeat every chromosome pair presented a similar pattern, allowing karyotypic analysis with all the SSRs tested. Our study is the first in mollusks to show the application of SSR in situ hybridization for chromosome identification and karyotyping. This technique can be a useful tool for oyster comparative studies and to understand genome organization in different oyster taxa.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, (IBB/CGB-UTAD), 5001-801 Vila Real, Portugal. kbouilly@yahoo.fr
authors.forename: K
authors.initials: K
authors.lastname: Bouilly
authors.forename: R
authors.initials: R
authors.lastname: Chaves
authors.forename: A
authors.initials: A
authors.lastname: Leitão
authors.forename: A
authors.initials: A
authors.lastname: Benabdelmouna
authors.forename: H
authors.initials: H
authors.lastname: Guedes-Pinto
mesh_terms: D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D002875:Chromosomes / Q000235:genetics*; D049896:Crassostrea / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D018598:Minisatellite Repeats / Q000235:genetics*
references.citation: Ann Clin Lab Sci. 1998 Jan-Feb;28(1):51-6
references.pmid: 9512785
references.citation: Genetics. 2004 Sep;168(1):351-62
references.pmid: 15454548
references.citation: Nat Genet. 1998 Apr;18(4):338-43
references.pmid: 9537415
references.citation: Micron. 2005;36(4):351-7
references.pmid: 15857774
references.citation: Mar Biotechnol (NY). 2005 May-Jun;7(3):207-14
references.pmid: 15933900
references.citation: Chromosoma. 1998 Dec;107(8):587-94
references.pmid: 9933412
references.citation: Genome. 2001 Aug;44(4):735-7
references.pmid: 11550911
references.citation: Genome. 2004 Oct;47(5):781-8
references.pmid: 15499392
references.citation: Science. 1996 Oct 25;274(5287):540-6
references.pmid: 8849440
references.citation: Hereditas. 2002;137(2):101-6
references.pmid: 12627834
references.citation: Hereditas. 1998;128(3):189-94
references.pmid: 9760868
references.citation: Nature. 1986 Aug 14-20;322(6080):652-6
references.pmid: 3748144
references.citation: Cytogenet Genome Res. 2002;96(1-4):113-6
references.pmid: 12438786
references.citation: Exp Cell Res. 1972 Nov;75(1):304-6
references.pmid: 4117921
references.citation: Nucleic Acids Res. 1989 Dec 11;17(23):10128
references.pmid: 2602131
references.citation: Genetica. 2000;108(3):253-7
references.pmid: 11294611
references.citation: Chromosome Res. 2006;14(3):243-51
references.pmid: 16628495
references.citation: Nucleic Acids Res. 1984 May 25;12(10):4127-38
references.pmid: 6328411
references.citation: Theor Appl Genet. 1993 Feb;85(6-7):653-7
references.pmid: 24196032
references.citation: Biol Bull. 2004 Feb;206(1):46-54
references.pmid: 14977729
references.citation: Mar Biotechnol (NY). 2001 Sep;3(5):486-92
references.pmid: 14961342
references.citation: Heredity (Edinb). 2002 May;88(5):381-4
references.pmid: 11986875
references.citation: Mol Ecol. 2002 Jan;11(1):1-16
references.pmid: 11903900
references.citation: Electrophoresis. 1991 Feb-Mar;12(2-3):193-203
references.pmid: 2040266
references.citation: Cytogenet Genome Res. 2003;102(1-4):179-83
references.pmid: 14970699
references.citation: Gene. 1996 Feb 12;168(2):205-9
references.pmid: 8654945
references.citation: Mol Cell Biol. 1989 Mar;9(3):1173-82
references.pmid: 2725493
references.citation: Hereditas. 2005 Feb;142(2005):73-9
references.pmid: 16970615
references.citation: Nucleic Acids Res. 1993 Mar 11;21(5):1111-5
references.pmid: 8464696
references.citation: Nucleic Acids Res. 1992 Jan 25;20(2):211-5
references.pmid: 1741246
references.citation: Theor Appl Genet. 1995 Jul;91(1):160-8
references.pmid: 24169682
references.citation: Genome. 2005 Feb;48(1):177-80
references.pmid: 15729410
references.citation: Nucleic Acids Res. 1981 Feb 25;9(4):879-94
references.pmid: 7232206