Molecular combing.
This unit describes an important advance in fiber-FISH technology called molecular combing, in which single DNA molecules are bound by one or both ends to a surface and stretched in a uniform and parallel manner by a receding meniscus. This technique is gentle on the molecules, rapid, and easy to perform. Reliable, quantitative information for genome-wide studies can be obtained without the need for other techniques and a large number of accurate measurements can be made in a single experiment. The authors provide detailed protocols for basic molecular combing, high-resolution physical mapping, and gene-dosage approaches as well as support protocols outlining surface preparation, DNA solution preparation, and probe labeling.
Conti
C
C
Institut Pasteur, Paris, France.
Caburet
S
S
Schurra
C
C
Bensimon
A
A
D000818:Animals; D004247:DNA / Q000032:analysis / Q000235:genetics; D015342:DNA Probes / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis / Q000494:pharmacology; D005821:Genetic Techniques; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry
D015342:DNA Probes; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D004247:DNA