title: Insights into the pathobiology of Paracoccidioides brasiliensis from transcriptome analysis--advances and perspectives.
abstract: Paracoccidioiddes brasiliensis is a thermodimorphic fungus endemic to Latin America, where it causes the most prevalent systemic mycosis, paracoccidioidomycosis (PCM). DNA microarray technology has been used to identify patterns of gene expression when a microbe is confronted with conditions of interest, such as in vitro and/or ex vivo interaction with specific cells. P. brasiliensis is one organism that has benefited from this approach. Even though its genome has not been sequenced yet, much has been discovered from its transcriptome and DNA array analyses. In this review, we will outline the current knowledge in P. brasiliensis transcriptome, with focus on differential expression analysis in vitro and on the discussion of the genes that are controlled during the host-pathogen interaction ex vivo in order to give insights into the pathobiology of this fungus. In vitro experiments enabled the delineation of whole metabolic pathways; the description of differential metabolism between mycelium and yeast cells and of the mainly signaling pathways controlling dimorphism, high temperature growth, thermal and oxidative stress, and virulence/ pathogenicity. Recent ex vivo experiments provided advances on the comprehension of the plasticity of response and indicate that P. brasiliensis is not only able to undergo fast and dramatic expression profile changes but can also discern subtle differences, such as whether it is being attacked by a macrophage or submitted to the bloodstream route conditions.
authors.affiliation: Laboratório de Biologia Molecular, Departamento de Biologia Celular, Universidade de Brasília, Brasilia, DF 70910-900, Brazil.
authors.forename: Simoneide S
authors.initials: SS
authors.lastname: Silva
authors.forename: Hugo C
authors.initials: HC
authors.lastname: Paes
authors.forename: Célia M A
authors.initials: CM
authors.lastname: Soares
authors.forename: Larissa
authors.initials: L
authors.lastname: Fernandes
authors.forename: Maria Sueli S
authors.initials: MS
authors.lastname: Felipe
mesh_terms: D000818:Animals; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010228:Paracoccidioides / Q000472:pathogenicity / Q000502:physiology; D010229:Paracoccidioidomycosis / Q000382:microbiology; D014774:Virulence
chemical_list: D005656:Fungal Proteins
references.citation: Mol Microbiol. 2003 Mar;47(6):1523-43
references.pmid: 12622810
references.citation: Infect Immun. 2006 Apr;74(4):2353-65
references.pmid: 16552065
references.citation: Mol Biol Cell. 2004 Feb;15(2):456-67
references.pmid: 14617819
references.citation: Mol Cell Biol. 2005 Dec;25(23):10611-27
references.pmid: 16287872
references.citation: Fungal Genet Biol. 2003 Jun;39(1):60-9
references.pmid: 12742064
references.citation: Mol Genet Genomics. 2006 Nov;276(5):450-63
references.pmid: 16924544
references.citation: Microbiol Mol Biol Rev. 2003 Dec;67(4):657-85
references.pmid: 14665679
references.citation: FEMS Immunol Med Microbiol. 2003 Nov 28;39(2):163-72
references.pmid: 14625100
references.citation: BMC Microbiol. 2007 Apr 10;7:29
references.pmid: 17425801
references.citation: Eukaryot Cell. 2005 Aug;4(8):1420-33
references.pmid: 16087747
references.citation: Curr Top Med Mycol. 1993;5:115-49
references.pmid: 8242798
references.citation: Microbes Infect. 2006 Oct;8(12-13):2686-97
references.pmid: 16962356
references.citation: Nature. 2005 Dec 22;438(7071):1105-15
references.pmid: 16372000
references.citation: Microbes Infect. 2008 Jan;10(1):12-20
references.pmid: 18096424
references.citation: Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1366-70
references.pmid: 17227865
references.citation: Infect Immun. 2005 Jun;73(6):3714-24
references.pmid: 15908401
references.citation: Eukaryot Cell. 2004 Oct;3(5):1076-87
references.pmid: 15470236
references.citation: Genet Mol Res. 2005 Jun 30;4(2):372-89
references.pmid: 16110452
references.citation: Rev Inst Med Trop Sao Paulo. 2005 Sep-Oct;47(5):257-62
references.pmid: 16302108
references.citation: Infect Immun. 2006 Feb;74(2):1323-38
references.pmid: 16428782
references.citation: Am J Pathol. 2000 May;156(5):1811-20
references.pmid: 10793093
references.citation: Infect Genet Evol. 2006 Sep;6(5):344-51
references.pmid: 16473563
references.citation: FEBS Lett. 2005 Oct 31;579(26):5950-7
references.pmid: 16137680
references.citation: Microbes Infect. 2007 Apr;9(5):583-90
references.pmid: 17387029
references.citation: J Clin Microbiol. 1995 Feb;33(2):505-7
references.pmid: 7714219
references.citation: Microbes Infect. 2003 Feb;5(2):107-13
references.pmid: 12650768
references.citation: J Med Vet Mycol. 1988;26(5):269-76
references.pmid: 3236146
references.citation: Infect Immun. 2003 Mar;71(3):1125-33
references.pmid: 12595423
references.citation: J Clin Microbiol. 1998 Mar;36(3):742-7
references.pmid: 9508305
references.citation: Microbiology (Reading). 2004 Oct;150(Pt 10):3315-26
references.pmid: 15470111
references.citation: Mol Biol Evol. 2006 Jan;23(1):65-73
references.pmid: 16151188
references.citation: J Clin Microbiol. 2006 Jun;44(6):2153-7
references.pmid: 16757613
references.citation: Science. 2006 Apr 28;312(5773):583-8
references.pmid: 16645097
references.citation: J Biol Chem. 2005 Jul 1;280(26):24706-14
references.pmid: 15849188
references.citation: BMC Genomics. 2006 Aug 14;7:208
references.pmid: 16907987
references.citation: Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1503-8
references.pmid: 11805289
references.citation: Sabouraudia. 1985 Apr;23(2):85-92
references.pmid: 4012515
references.citation: Mycopathologia. 1977 Dec 16;62(2):77-86
references.pmid: 340954
references.citation: Genet Mol Res. 2005 Jun 30;4(2):326-45
references.pmid: 16110449
references.citation: Eukaryot Cell. 2003 Feb;2(1):34-48
references.pmid: 12582121
references.citation: Eukaryot Cell. 2005 Dec;4(12):2115-28
references.pmid: 16339729
references.citation: Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):972-7
references.pmid: 11805339
references.citation: Infect Immun. 1989 Aug;57(8):2289-94
references.pmid: 2744848
references.citation: J Med Vet Mycol. 1987 Oct;25(5):343-5
references.pmid: 2448446
references.citation: Infect Immun. 1988 Nov;56(11):2997-3000
references.pmid: 3169995
references.citation: Microbiology (Reading). 2005 May;151(Pt 5):1325-1340
references.pmid: 15870443
references.citation: Fungal Genet Biol. 2007 Jan;44(1):25-31
references.pmid: 16879998
references.citation: Yeast. 2003 Feb;20(3):263-71
references.pmid: 12557278
references.citation: Mol Genet Genomics. 2004 Jul;271(6):667-77
references.pmid: 15138890
references.citation: Res Microbiol. 2004 May;155(4):245-51
references.pmid: 15142621
references.citation: Genome Res. 2003 Jun;13(6B):1301-6
references.pmid: 12819127