title: Class III beta-tubulin is a component of the mitotic spindle in multiple cell types.
abstract: The findings of this study show that Class III beta-tubulin is a component of the mitotic spindle in multiple cell types. Class III beta-tubulin has been widely used as a neuron-specific marker, but it has been detected also in association with breast and pancreatic cancers. In this study, we describe a novel finding of Class III beta-tubulin in a subpopulation of cells in malignant peripheral nerve sheath tumor. The findings of this study also show that Class III beta-tubulin is expressed by normal mesenchymal and epithelial cells (fibroblasts and keratinocytes), two transitional cell carcinoma cell lines, and neurofibroma Schwann cells, as shown by immunolabeling and Western transfer analysis using two different Tuj-1 antibodies that are specific for Class III beta-tubulin. The corresponding mRNA was detected using RT-PCR and whole human genome microarrays. Both antibodies localized Class III beta-tubulin to the mitotic spindle and showed a colocalization with alpha-tubulin. The immunoreaction became visible in early prophase, and the most intense immunoreaction was detected during metaphase and anaphase when microtubules were connected to the kinetochores on chromosomes. Class III beta-tubulin-specific immunoreaction lasted to the point when the midbody of cytokinesis became detectable.
authors.affiliation: Department of Cell Biology and Anatomy, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland.
authors.forename: Eeva-Mari
authors.initials: EM
authors.lastname: Jouhilahti
authors.forename: Sirkku
authors.initials: S
authors.lastname: Peltonen
authors.forename: Juha
authors.initials: J
authors.lastname: Peltonen
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002295:Carcinoma, Transitional Cell; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D018613:Microscopy, Confocal; D008938:Mitosis; D018317:Nerve Sheath Neoplasms / Q000378:metabolism; D009455:Neurofibroma; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*; D014404:Tubulin / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D014404:Tubulin
references.citation: J Neurobiol. 1995 May;27(1):26-43
references.pmid: 7643073
references.citation: Cell Motil Cytoskeleton. 2003 Jun;55(2):77-96
references.pmid: 12740870
references.citation: Folia Histochem Cytobiol. 2007;45(1):41-5
references.pmid: 17378245
references.citation: J Cancer Res Ther. 2005 Apr-Jun;1(2):92-7
references.pmid: 17998634
references.citation: J Neuropathol Exp Neurol. 1993 Nov;52(6):655-66
references.pmid: 8229085
references.citation: Cancer Res. 2005 Mar 15;65(6):2397-405
references.pmid: 15781655
references.citation: Lab Invest. 2002 Mar;82(3):353-61
references.pmid: 11896214
references.citation: Annu Rev Cell Biol. 1988;4:687-716
references.pmid: 3058169
references.citation: Clin Cancer Res. 2003 Aug 1;9(8):2992-7
references.pmid: 12912947
references.citation: J Cell Sci. 2001 Aug;114(Pt 15):2723-33
references.pmid: 11683407
references.citation: J Invest Dermatol. 1998 Mar;110(3):232-7
references.pmid: 9506441
references.citation: J Neurosci Res. 2000 Sep 1;61(5):524-32
references.pmid: 10956422
references.citation: Cell Mol Life Sci. 2005 Dec;62(24):3039-56
references.pmid: 16314924
references.citation: Int Rev Cytol. 1998;178:207-75
references.pmid: 9348671
references.citation: Int Rev Cytol. 2008;265:111-58
references.pmid: 18275887
references.citation: Histopathology. 2007 Oct;51(4):539-46
references.pmid: 17714470
references.citation: J Neurosci. 1993 Jan;13(1):285-99
references.pmid: 8423474
references.citation: Exp Cell Res. 2004 Aug 1;298(1):218-28
references.pmid: 15242776
references.citation: J Child Neurol. 2003 Dec;18(12):851-66; discussion 867
references.pmid: 14736079