title: Radiolytic ligation of oligodeoxynucleotides possessing disulfide bond.
abstract: Hypoxic X-radiolysis of diluted aqueous solutions was performed to generate hydrated electrons that induced one-electron reduction of oligodeoxynucleotides (ODNs) possessing a disulfide bond. Upon hypoxic irradiation, reductive ligation reaction between two types of ODNs occurred in the presence of a template ODN strand, resulting in the formation of a prescribed ODN in substantial amounts.
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan. tanabeka@scl.kyoto-u.ac.jp
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tanabe
authors.forename: Emi
authors.initials: E
authors.lastname: Kuraseko
authors.forename: Eiji
authors.initials: E
authors.lastname: Matsumoto
authors.forename: Hisatsugu
authors.initials: H
authors.lastname: Yamada
authors.forename: Takeo
authors.initials: T
authors.lastname: Ito
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Nishimoto
mesh_terms: D004220:Disulfides / Q000737:chemistry*; D004583:Electrons; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000528:radiation effects; D010084:Oxidation-Reduction; D014965:X-Rays
chemical_list: D004220:Disulfides; D009838:Oligodeoxyribonucleotides