[Molecular basics of aldosterone and cortisol synthesis in normal adrenals and adrenocortical adenomas].
The aim of the study is to present genes encoding enzymatic proteins of aldosterone and cortisol synthesis pathway, methods of their transcriptional activity measurement, mRNA expression of the genes in normal adrenal cortex, in adrenocortical adenomas excised from patients with Conn and Cushing syndromes, as well as in adrenocortical adenomas excised from patients, in which hormonal activity of the tumour was not confirmed. According to presented papers mRNA expression of analyzed genes is best known in tissue obtained from tumours excised from patients with Conn syndrome. On the other hand transcriptional activity of the genes within the other adrenocortical adenomas is documented in lesser degree. It concerns particularly analyses of tissue material obtained from patients, in which hormonal activity of adrenal tumours was not confirmed with biochemical tests. It should be also considered, that the frame of reference for the majority of molecular analyses of adrenocortical tumour tissues was material obtained from little number of normal adrenals, what decreases in some degree credibility of obtained results. Mentioned above remarks may be the basis for conduction of further investigations based on larger material, obtained both from normal adrenals and adrenocortical adenomas.
Ziaja
Jacek
J
Katedra i Klinika Chirurgii Ogolnej, Naczyniowej i Transplantacyjnej, Slaski Uniwersytet Medyczny, Katowice, Poland. ziacek@mp.pl
Cholewa
Krzysztof
K
Mazurek
Urszula
U
Cierpka
Lech
L
D000302:Adrenal Cortex / Q000378:metabolism; D000306:Adrenal Cortex Neoplasms / Q000201:enzymology* / Q000235:genetics; D000311:Adrenal Glands / Q000378:metabolism*; D018246:Adrenocortical Adenoma / Q000201:enzymology* / Q000235:genetics; D000450:Aldosterone / Q000096:biosynthesis* / Q000235:genetics; D003480:Cushing Syndrome / Q000235:genetics / Q000378:metabolism; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D006854:Hydrocortisone / Q000096:biosynthesis* / Q000235:genetics; D006929:Hyperaldosteronism / Q000235:genetics / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012016:Reference Values; D014158:Transcription, Genetic
D012333:RNA, Messenger; D000450:Aldosterone; D006899:Mixed Function Oxygenases; D019405:Cytochrome P-450 CYP11B2; D006854:Hydrocortisone