Distribution and dynamic pathway of selenium species in selenium-deficient mice injected with (82)Se-enriched selenite.
In order to elucidate Se metabolism in a living body, (82)Se-enriched selenite was injected intravenously into mice fed Se-adequate and -deficient diets. We studied the time-dependent changes in the distribution of the labeled Se in organs, red blood cells, and plasma. The total Se was determined by flow-injection ICPMS, and Se speciation analysis was conducted by micro-affinity chromatography coupled with low-flow ICPMS. Total Se in almost all organs, including liver, showed the maximum at 1 h after injection. From speciation analysis, exogenous (82)Se as Se-containing proteins other than selenoprotein P (Sel-P) (selenium containing albumin (SeAlb) and extra cellular glutathione peroxidase (eGPx)), peaked at 1 h and quickly decreased from 1 to 6 h after injection, whereas that as Sel-P, peaked at 6 h, and gradually decreased from 6 to 72 h after injection. We found that there were two pathways for the transfer of Se in mice; one was as SeAlb until 1 h after injection, and the other was as Sel-P from 6 to 72 h after injection.
Shigeta
Kaori
K
Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Bunkyo, Tokyo 112-8551, Japan.
Matsumura
Kentaro
K
Suzuki
Yoshinari
Y
Shinohara
Atsuko
A
Furuta
Naoki
N
D000818:Animals; D002417:Cattle; D004032:Diet; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007267:Injections; D007554:Isotopes; D008297:Male; D051379:Mice; D015203:Reproducibility of Results; D012643:Selenium / Q000097:blood / Q000172:deficiency / Q000378:metabolism* / Q000493:pharmacokinetics*; D018038:Sodium Selenite / Q000008:administration & dosage* / Q000378:metabolism* / Q000493:pharmacokinetics; D013997:Time Factors; D014018:Tissue Distribution
D007554:Isotopes; D012643:Selenium; D018038:Sodium Selenite