[Determination of hyoscyamine and scopolamine in serum and urine of humans by liquid chromatography with tandem mass spectrometry].
A simple method was developed for the analysis of hyoscyamine and scopolamine in human serum and urine using liquid chromatography with tandem mass spectrometry (LC/MS/MS). Hyoscyamine and scopolamine in serum and urine were cleaned up with an Oasis HLB cartridge and a PSA cartridge. The LC separation was carried out on an ODS column, using linear gradient elution with 5 mmol/L IPCC-MS3-methanol as the mobile phase. The mass spectral acquisition was done in the positive ion mode by applying selected reaction monitoring (SRM). The recoveries of hyoscyamine and scopolamine were 86.0-105% from human serum and urine fortified at 0.2 ng/mL and 10 ng/mL. The detection limits of hyoscyamine and scopolamine were 0.02 ng/mL. Four serum and three urine samples of humans poisoned by eating Datura innoxia Mill. were analyzed by this method. Hyoscyamine and scopolamine were detected at the levels of 0.45-3.5 ng/mL in all serum samples and 170-670 ng/mL in all urine samples.
Konishi
Tomohiko
T
Fukuoka City Institute for Hygiene and the Environment. Funuoka, Japan.
Akaki
Kouichi
K
Hatano
Kazuhiro
K
D000368:Aged; D000369:Aged, 80 and over; D001285:Atropine / Q000032:analysis* / Q000097:blood / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods*; D032302:Datura; D005260:Female; D006801:Humans; D008297:Male; D010939:Plant Poisoning / Q000378:metabolism; D012601:Scopolamine / Q000032:analysis* / Q000097:blood / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods*
D001285:Atropine; D012601:Scopolamine