title: Microcrystalline cellulose induces time-dependent lung functional and inflammatory changes.
abstract: We determined whether microcrystalline cellulose (MCC), a component of pharmaceutical tablets, induces pulmonary changes. In vivo [resistive and viscoelastic pressures (DeltaP(1) and DeltaP(2)), static elastance (E(L))] and in vitro [tissue resistance (R), elastance (E), and hysteresivity (eta)] lung mechanics, histology, and bronchoalveolar lavage fluid (BALF) were analyzed 3h, 24h, and 3, 15 and 30 days after intratracheal instillation of saline (C) or MCC in BALB/c mice. DeltaP(1) increased at 3h, remaining higher than C until day 3, while E(L) and DeltaP(2) increased only at 24h. At 3 days all mechanical parameters returned to baseline. R and E increased only at 24h. MCC increased alveolar collapse and the number of neutrophils in BALF at 3h, until 3 and 15 days, respectively. At 3 days MCC migrate from the airways into the parenchyma, where they were observed until 30 days. In conclusion, microcrystalline cellulose yielded an acute and self-limited inflammation that impaired lung mechanics.
authors.affiliation: Laboratory of Respiration Physiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: L K S
authors.initials: LK
authors.lastname: Nagato
authors.forename: M G F
authors.initials: MG
authors.lastname: Lourenço
authors.forename: R A
authors.initials: RA
authors.lastname: Cadete
authors.forename: J H P
authors.initials: JH
authors.lastname: Leite-Júnior
authors.forename: V L G
authors.initials: VL
authors.lastname: Koatz
authors.forename: P R M
authors.initials: PR
authors.lastname: Rocco
authors.forename: D S
authors.initials: DS
authors.lastname: Faffe
authors.forename: W A
authors.initials: WA
authors.lastname: Zin
mesh_terms: D000403:Airway Resistance / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D002482:Cellulose / Q000009:adverse effects*; D005079:Excipients / Q000009:adverse effects*; D007249:Inflammation / Q000139:chemically induced* / Q000503:physiopathology; D016014:Linear Models; D008168:Lung / Q000473:pathology* / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011650:Pulmonary Alveoli / Q000473:pathology; D001261:Pulmonary Atelectasis / Q000139:chemically induced; D011897:Random Allocation; D015656:Respiratory Mechanics; D013997:Time Factors
chemical_list: D005079:Excipients; D002482:Cellulose; C109691:microcrystalline cellulose