title: Putative outer membrane proteins of Leptospira interrogans stimulate human umbilical vein endothelial cells (HUVECS) and express during infection.
abstract: Cell adhesion molecules (CAMs) are surface receptors present in eukaryotic cells that mediate cell-cell or cell-extracellular matrix interactions. Vascular endothelium stimulation in vitro that lead to the upregulation of CAMs was reported for the pathogenic spirochaetes, including rLIC10365 of Leptospira interrogans. In this study, we report the cloning of LIC10507, LIC10508, LIC10509 genes of L. interrogans using Escherichia coli as a host system. The rational for selecting these sequences is due to their location in L. interrogans serovar Copenhageni genome that has a potential involvement in pathogenesis. The genes encode for predicted lipoproteins with no assigned functions. The purified recombinant proteins were capable to promote the upregulation of intercellular adhesion molecule 1 (ICAM-1) and E-selectin on monolayers of human umbilical vein endothelial cells (HUVECS). In addition, the coding sequences are expressed in the renal tubules of animal during bacterial experimental infection. The proteins are probably located at the outer membrane of the bacteria since they are detected in detergent-phase of L. interrogans Triton X-114 extract. Altogether our data suggest a possible involvement of these proteins during bacterial infection and provide new insights into the role of this region in the pathogenesis of Leptospira.
authors.affiliation: Instituto de Biotecnología y Biología Molecular, CCT-La Plata, CONICET-UNLP, La Plata, Argentina.
authors.forename: Ricardo M
authors.initials: RM
authors.lastname: Gómez
authors.forename: Monica L
authors.initials: ML
authors.lastname: Vieira
authors.forename: Mirta
authors.initials: M
authors.lastname: Schattner
authors.forename: Elisa
authors.initials: E
authors.lastname: Malaver
authors.forename: Monica M
authors.initials: MM
authors.lastname: Watanabe
authors.forename: Angela S
authors.initials: AS
authors.lastname: Barbosa
authors.forename: Patricia A E
authors.initials: PA
authors.lastname: Abreu
authors.forename: Zenaide M
authors.initials: ZM
authors.lastname: de Morais
authors.forename: Javier O
authors.initials: JO
authors.lastname: Cifuente
authors.forename: Marina V
authors.initials: MV
authors.lastname: Atzingen
authors.forename: Tatiane R
authors.initials: TR
authors.lastname: Oliveira
authors.forename: Silvio A
authors.initials: SA
authors.lastname: Vasconcellos
authors.forename: Ana L T O
authors.initials: AL
authors.lastname: Nascimento
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002478:Cells, Cultured; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism* / Q000382:microbiology; D015870:Gene Expression; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D007921:Leptospira interrogans / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D007922:Leptospirosis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1