title: African swine fever virus protein p30 interaction with heterogeneous nuclear ribonucleoprotein K (hnRNP-K) during infection.
abstract: Heterogeneous nuclear ribonucleoprotein K (hnRNP-K) was identified as interacting cellular protein with the abundant immediate early protein p30 from African swine fever virus (ASFV) in a macrophage cDNA library screening. The interacting regions of hnRNP-K with p30 were established within residues 35-197, which represent KH1 and KH2 domains responsible for RNA binding. Colocalization of hnRNP-K and p30 was observed mainly in the nucleus, but not in the cytoplasm of infected cells and infection modified hnRNP-K subcellular distribution and decreased the incorporation of 5-fluorouridine into nascent RNA. Since similar effects were observed in cells transiently expressing p30, this interaction provides new insights into p30 function and could represent a possible additional mechanism by which ASFV downregulates host cell mRNA translation.
authors.affiliation: Departamento de Biotecnología, INIA, Carretera de la Coruña Km 7, 28040 Madrid, Spain.
authors.forename: Bruno
authors.initials: B
authors.lastname: Hernaez
authors.forename: Jose M
authors.initials: JM
authors.lastname: Escribano
authors.forename: Covadonga
authors.initials: C
authors.lastname: Alonso
mesh_terms: D000357:African Swine Fever / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D015723:Gene Library; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D013552:Swine / Q000378:metabolism / Q000821:virology; D020798:Two-Hybrid System Techniques; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D010750:Phosphoproteins; D012333:RNA, Messenger; D014764:Viral Proteins; C074960:P30 protein, African Swine Fever Virus
references.citation: J Virol Methods. 1997 Feb;64(1):27-35
references.pmid: 9029527
references.citation: J Biol Chem. 1998 Jul 10;273(28):17651-9
references.pmid: 9651361
references.citation: Mol Biol Rep. 1993 Aug;18(2):73-8
references.pmid: 8232298
references.citation: Acta Virol. 2001;45(5-6):269-77
references.pmid: 12083325
references.citation: J Biol Chem. 1995 Nov 10;270(45):26976-85
references.pmid: 7592945
references.citation: Virology. 2007 Oct 10;367(1):212-21
references.pmid: 17561226
references.citation: Science. 1998 Jul 24;281(5376):562-5
references.pmid: 9677199
references.citation: Nucleic Acids Res. 2001 Feb 1;29(3):638-43
references.pmid: 11160884
references.citation: J Virol. 2007 Mar;81(6):2923-9
references.pmid: 17215279
references.citation: DNA Cell Biol. 2001 Sep;20(9):569-77
references.pmid: 11747608
references.citation: FEBS Lett. 1997 Feb 17;403(2):113-5
references.pmid: 9042948
references.citation: Proteomics. 2001 Nov;1(11):1447-56
references.pmid: 11922604
references.citation: Bioessays. 2004 Jun;26(6):629-38
references.pmid: 15170860
references.citation: Virology. 1988 May;164(1):280-3
references.pmid: 3363868
references.citation: Eur J Biochem. 1996 Oct 15;241(2):425-31
references.pmid: 8917439
references.citation: Virology. 1998 Apr 10;243(2):461-71
references.pmid: 9568043
references.citation: Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9044-9
references.pmid: 11470915
references.citation: J Virol. 2001 Oct;75(20):9819-27
references.pmid: 11559815
references.citation: Proteomics. 2004 Jul;4(7):2037-46
references.pmid: 15221765
references.citation: J Virol. 1993 May;67(5):2475-85
references.pmid: 8474154
references.citation: Mol Cell Biol. 1996 May;16(5):2350-60
references.pmid: 8628302
references.citation: J Virol. 1996 Aug;70(8):5689-94
references.pmid: 8764090
references.citation: Virology. 2008 Jun 5;375(2):561-72
references.pmid: 18329683
references.citation: Cell. 1994 Apr 8;77(1):33-9
references.pmid: 8156595
references.citation: Proteomics. 2006 Apr;6(8):2395-406
references.pmid: 16518874
references.citation: J Vet Diagn Invest. 1990 Jul;2(3):191-6
references.pmid: 2094444
references.citation: Arch Virol. 1987;92(3-4):221-32
references.pmid: 3101652
references.citation: EMBO J. 2001 Jan 15;20(1-2):240-9
references.pmid: 11226174
references.citation: J Cell Biol. 2000 Jan 24;148(2):283-92
references.pmid: 10648561
references.citation: Virology. 1992 Jul;189(1):368-73
references.pmid: 1604821
references.citation: J Biol Chem. 1999 Oct 8;274(41):28991-8
references.pmid: 10506147