Synthesis and properties of oligonucleotides containing silylated pyrene derivatives.
Preparation and properties of the novel fluorescently labelled oligonucleotides, containing the silylated pyrene derivatives are reported. The silylated pyrene derivatives were introduced into both 5'-terminus and C-5 position of the deoxyuridine derivatives. The fluorescent spectra indicated the possibility of the discrimination of the duplex formation by the simple denaturation.
Moriguchi
Tomohisa
T
Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Tenjincho 1-5-1, Kiryu, Gunma, 376-8515, Japan.
Hattori
Masayoshi
M
Sekiguchi
Tohru
T
Ichimura
Mayumi
M
Kato
Takahiko
T
Shinozuka
Kazuo
K
D005456:Fluorescent Dyes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*; D013936:Thymidine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009943:Organophosphorus Compounds; D011721:Pyrenes; D013936:Thymidine