title: The R2R3 MYB transcription factor GhMYB109 is required for cotton fiber development.
abstract: Cotton (Gossypium hirsutum L.) fibers are single highly elongated cells derived from the outer epidermis of ovules. A large number of genes are required for fiber differentiation and development, but so far, little is known about how these genes control and regulate the process of fiber development. Here we examine the role of the cotton-fiber-specific R2R3 MYB gene GhMYB109 in cotton fiber development. Transgenic reporter gene analysis revealed that a 2-kb GhMYB109 promoter was sufficient to confirm its fiber-specific expression. Antisense-mediated suppression of GhMYB109 led to a substantial reduction in fiber length. Consistently, several genes related to cotton fiber growth were found to be significantly reduced in the transgenic cotton. Our results showed that GhMYB109 is required for cotton fiber development and reveal a largely conserved mechanism of the R2R3 MYB transcription factor in cell fate determination in plants.
authors.affiliation: Institute of Cotton Research, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, Shanxi Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Pu
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Fan
authors.forename: Weicai
authors.initials: W
authors.lastname: Yang
authors.forename: Yongbiao
authors.initials: Y
authors.lastname: Xue
mesh_terms: D032561:Cotton Fiber; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development*; D008855:Microscopy, Electron, Scanning; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D014157:Transcription Factors
references.citation: Plant J. 1993 Apr;3(4):553-62
references.pmid: 8220462
references.citation: Nucleic Acids Res. 2003 May 15;31(10):2534-43
references.pmid: 12736302
references.citation: Nature. 1994 Jun 23;369(6482):661-4
references.pmid: 8208293
references.citation: Plant Mol Biol. 2005 Jan;57(1):67-81
references.pmid: 15821869
references.citation: Plant Cell. 1999 Jun;11(6):1105-16
references.pmid: 10368181
references.citation: Mol Gen Genet. 1999 Jun;261(4-5):660-71
references.pmid: 10394903
references.citation: Plant J. 1998 Oct;16(2):263-76
references.pmid: 9839469
references.citation: Plant Cell. 2006 Mar;18(3):651-64
references.pmid: 16461577
references.citation: Plant J. 1998 Jan;13(1):39-50
references.pmid: 9680963
references.citation: Plant Cell. 2005 Mar;17(3):859-75
references.pmid: 15722467
references.citation: Plant Mol Biol. 2004 May;55(3):389-98
references.pmid: 15604688
references.citation: J Exp Bot. 2000 Mar;51(344):497-505
references.pmid: 10938806
references.citation: Curr Opin Plant Biol. 2001 Oct;4(5):447-56
references.pmid: 11597504
references.citation: BMC Plant Biol. 2007 May 16;7:22
references.pmid: 17506877
references.citation: Planta. 2006 Feb;223(3):418-32
references.pmid: 16254724
references.citation: Plant Mol Biol. 2004 Dec;56(6):929-45
references.pmid: 15821991
references.citation: Plant Cell Physiol. 2006 Jan;47(1):107-27
references.pmid: 16278222
references.citation: Plant Mol Biol. 2004 Mar;54(4):471-87
references.pmid: 15316284
references.citation: Development. 1997 May;124(9):1789-98
references.pmid: 9165126
references.citation: Development. 1998 Sep;125(17):3497-508
references.pmid: 9693152
references.citation: Development. 2005 Jan;132(2):359-70
references.pmid: 15604096
references.citation: Dev Biol. 2004 Apr 15;268(2):506-13
references.pmid: 15063185
references.citation: Annu Rev Plant Biol. 2003;54:403-30
references.pmid: 14502997
references.citation: Genome Res. 2006 Mar;16(3):441-50
references.pmid: 16478941
references.citation: Plant Mol Biol. 2004 Apr;54(6):911-29
references.pmid: 15604659
references.citation: Plant Physiol. 2001 Dec;127(4):1361-6
references.pmid: 11743074
references.citation: Genes Dev. 1994 Jun 15;8(12):1388-99
references.pmid: 7926739
references.citation: Plant Physiol. 2002 Oct;130(2):666-74
references.pmid: 12376634
references.citation: EMBO J. 2002 Oct 1;21(19):5036-46
references.pmid: 12356720
references.citation: Trends Plant Sci. 2005 Feb;10(2):63-70
references.pmid: 15708343
references.citation: Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12768-73
references.pmid: 11038522
references.citation: Trends Plant Sci. 2006 Jun;11(6):274-80
references.pmid: 16697247
references.citation: Plant Cell. 2004 Sep;16(9):2323-34
references.pmid: 15316114
references.citation: Development. 1998 Apr;125(7):1161-71
references.pmid: 9477315
references.citation: Biochim Biophys Acta. 2003 Oct 20;1630(1):25-34
references.pmid: 14580676
references.citation: Nat Rev Mol Cell Biol. 2004 Jun;5(6):471-80
references.pmid: 15173826
references.citation: Curr Opin Plant Biol. 2003 Feb;6(1):74-8
references.pmid: 12495754
references.citation: Plant J. 2006 Sep;47(5):761-75
references.pmid: 16889650
references.citation: Plant J. 2002 Feb;29(3):359-69
references.pmid: 11844112
references.citation: Philos Trans R Soc Lond B Biol Sci. 2002 Jun 29;357(1422):809-13
references.pmid: 12079676