PAMP-induced defense responses in potato require both salicylic acid and jasmonic acid.
To elucidate the molecular mechanisms underlying pathogen-associated molecular pattern (PAMP)-induced defense responses in potato (Solanum tuberosum), the role of the signaling compounds salicylic acid (SA) and jasmonic acid (JA) was analyzed. Pep-13, a PAMP from Phytophthora, induces the accumulation of SA, JA and hydrogen peroxide, as well as the activation of defense genes and hypersensitive-like cell death. We have previously shown that SA is required for Pep-13-induced defense responses. To assess the importance of JA, RNA interference constructs targeted at the JA biosynthetic genes, allene oxide cyclase and 12-oxophytodienoic acid reductase, were expressed in transgenic potato plants. In addition, expression of the F-box protein COI1 was reduced by RNA interference. Plants expressing the RNA interference constructs failed to accumulate the respective transcripts in response to wounding or Pep-13 treatment, neither did they contain significant amounts of JA after elicitation. In response to infiltration of Pep-13, the transgenic plants exhibited a highly reduced accumulation of reactive oxygen species as well as reduced hypersensitive cell death. The ability of the JA-deficient plants to accumulate SA suggests that SA accumulation is independent or upstream of JA accumulation. These data show that PAMP responses in potato require both SA and JA and that, in contrast to Arabidopsis, these compounds act in the same signal transduction pathway. Despite their inability to fully respond to PAMP treatment, the transgenic RNA interference plants are not altered in their basal defense against Phytophthora infestans.
Halim
Vincentius A
VA
Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, D-06120 Halle, Saale, Germany.
Altmann
Simone
S
Ellinger
Dorothea
D
Eschen-Lippold
Lennart
L
Miersch
Otto
O
Scheel
Dierk
D
Rosahl
Sabine
S
D016923:Cell Death; D003517:Cyclopentanes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism*; D055750:Phytophthora infestans / Q000254:growth & development; D010935:Plant Diseases / Q000235:genetics*; D010937:Plant Growth Regulators / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism*; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism*
D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; C011006:jasmonic acid; D006861:Hydrogen Peroxide; D020156:Salicylic Acid