Hydrophobic surfaces for enhanced differentiation of embryonic stem cell-derived embryoid bodies.
With their unique ability to differentiate into all cell types, embryonic stem (ES) cells hold great therapeutic promise. To improve the efficiency of embryoid body (EB)-mediated ES cell differentiation, we studied murine EBs on the basis of their size and found that EBs with an intermediate size (diameter 100-300 microm) are the most proliferative, hold the greatest differentiation potential, and have the lowest rate of cell death. In an attempt to promote the formation of this subpopulation, we surveyed several biocompatible substrates with different surface chemical parameters and identified a strong correlation between hydrophobicity and EB development. Using self-assembled monolayers of various lengths of alkanethiolates on gold substrates, we directly tested this correlation and found that surfaces that exhibit increasing hydrophobicity enrich for the intermediate-size EBs. When this approach was applied to the human ES cell system, similar phenomena were observed. Our data demonstrate that hydrophobic surfaces serve as a platform to deliver uniform EB populations and may significantly improve the efficiency of ES cell differentiation.
Valamehr
Bahram
B
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
Jonas
Steven J
SJ
Polleux
Julien
J
Qiao
Rong
R
Guo
Shuling
S
Gschweng
Eric H
EH
Stiles
Bangyan
B
Kam
Korey
K
Luo
Tzy-Jiun M
TJ
Witte
Owen N
ON
Liu
Xin
X
Dunn
Bruce
B
Wu
Hong
H
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D016895:Culture Media, Serum-Free; D004129:Dimethylpolysiloxanes / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice
D016895:Culture Media, Serum-Free; D004129:Dimethylpolysiloxanes; C013830:baysilon
Mol Cell Biol. 1993 Jan;13(1):473-86
8417345
Stem Cells Dev. 2006 Jun;15(3):295-303
16846368
Biomaterials. 2006 Jun;27(16):3044-63
16458351
Nat Neurosci. 2004 Sep;7(9):1003-9
15332090
J Embryol Exp Morphol. 1985 Jun;87:27-45
3897439
Science. 2001 May 18;292(5520):1389-94
11326082
Blood. 2005 Sep 1;106(5):1601-3
15914555
Cytometry. 1997 Jan 1;27(1):1-20
9000580
Nat Biotechnol. 2007 Dec;25(12):1477-82
18037879
Genes Dev. 2005 May 15;19(10):1129-55
15905405
Cell. 2008 Feb 22;132(4):661-80
18295582
Stem Cells Dev. 2007 Apr;16(2):213-22
17521233
Stem Cells. 2001;19(3):193-204
11359944
Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16806-11
17077151
Mol Biol Cell. 2001 Sep;12(9):2730-41
11553712
Trends Biotechnol. 2007 May;25(5):224-30
17379341
Development. 2004 Nov;131(22):5515-25
15509763
Biomaterials. 2006 Dec;27(36):6032-42
16884768
Biotechnol Bioeng. 2002 May 20;78(4):442-53
11948451
Stem Cells. 2007 Sep;25(9):2224-34
17585171
Cell. 2006 Aug 25;126(4):677-89
16923388
Annu Rev Cell Dev Biol. 2001;17:435-62
11687496
Biomed Microdevices. 2004 Sep;6(3):219-22
15377831
Nanotechnology. 2005 Oct;16(10):2420-6
20818029
Curr Opin Cell Biol. 1995 Dec;7(6):862-9
8608017
Nat Biotechnol. 2006 Nov;24(11):1402-11
17086172
Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11307-12
11027332
Stem Cells. 2007 Apr;25(4):929-38
17185609
Stem Cells. 2004;22(3):275-82
15153605
Blood. 2003 Jan 15;101(2):508-16
12393529
Chem Rev. 2005 Apr;105(4):1103-69
15826011
J Am Chem Soc. 2004 Sep 8;126(35):10808-9
15339142