Phenotypic and genotypic characterisation of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential.
Despite frequent reports on the presence of Blastocystis hominis in human intestinal tract, its pathogenicity remains a matter of intense debate. These discrepancies may be due to the varying pathogenic potential or virulence of the isolates studied. The present study represents the first to investigate both phenotypic and genotypic characteristics of B. hominis obtained from symptomatic and asymptomatic individuals. Symptomatic isolates had a significantly greater size range and lower growth rate in Jones' medium than asymptomatic isolates. The parasite cells of symptomatic isolates exhibited rougher surface topography and greater binding affinity to Canavalia ensiformis (ConA) and Helix pomatia (HPA). The present study also identifies further phenotypic characteristics, which aided in differentiating the pathogenic forms from the non-pathogenic forms of B. hominis. Blastocystis subtype 3 was found to be correlated well with the disease.
Tan
T C
TC
Department of Parasitology, University of Malaya, Kuala Lumpur, Malaysia.
Suresh
K G
KG
Smith
H V
HV
D000818:Animals; D016776:Blastocystis Infections / Q000469:parasitology* / Q000503:physiopathology*; D016845:Blastocystis hominis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D003470:Culture Media; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D005838:Genotype; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010274:Parasitology / Q000379:methods; D010641:Phenotype; D016133:Polymerase Chain Reaction
D003470:Culture Media; D016054:DNA, Protozoan
Int J Parasitol. 1996 May;26(5):527-32
8818733
Trop Med Int Health. 1997 Aug;2(8):771-8
9294547
Zentralbl Bakteriol. 1994 Jan;280(3):403-8
8167435
Parasitol Res. 1994;80(6):523-7
7809004
Int J Parasitol. 1989 Feb;19(1):43-56
2707962
Nat New Biol. 1973 Oct 10;245(145):186-7
4355103
Int J Parasitol. 2002 Jun 15;32(7):789-804
12062550
Parasitol Res. 1999 Dec;85(12):974-6
10599919
Adv Parasitol. 1993;32:1-70
8237614
Ann Trop Med Parasitol. 1946 Jul;40:130-40
20997895
Trop Med Int Health. 1997 Mar;2(3):245-53
9491103
Parasitol Res. 2006 Nov;99(6):737-42
16816959
Parasitol Res. 2006 Feb;98(3):189-93
16323025
Parasitol Res. 1999 Feb;85(2):93-7
9934956
Diagn Microbiol Infect Dis. 2007 Nov;59(3):303-7
17913433
Eur J Clin Microbiol Infect Dis. 2004 Jun;23(6):509-11
15168139
Ann Trop Med Parasitol. 1997 Mar;91(2):223-4
9307666
J Protozool. 1981 Nov;28(4):483-5
7198685
Am J Trop Med Hyg. 1997 Mar;56(3):301-6
9129532
Exp Parasitol. 2007 Jun;116(2):111-9
17266951
Parasitol Res. 2004 Jan;92(1):22-9
14598169
Fertil Steril. 2007 May;87(5):1200-7
17307173
Am J Trop Med Hyg. 2001 Oct;65(4):393-6
11693890
J Exp Med. 1977 Mar 1;145(3):652-65
233919
Exp Parasitol. 1974 Oct;36(2):233-43
4369819
Parasitol Res. 2006 Sep;99(4):459-65
16628457
Parasitol Res. 1994;80(6):505-11
7809001
Parasitology. 1996 Apr;112 ( Pt 4):363-9
8935948
Acta Derm Venereol. 2008;88(1):80-1
18176765
Parasitol Res. 2006 Oct;99(5):597-601
16688468
J Med Microbiol. 1995 Apr;42(4):304-7
7707340
Parasitology. 1997 Feb;114 ( Pt 2):95-104
9051918
Trends Parasitol. 2007 Mar;23(3):93-6
17241816
J Clin Microbiol. 2005 Jan;43(1):348-55
15634993
J Biol Chem. 1998 Jun 19;273(25):15838-45
9624184
Mol Biochem Parasitol. 1997 Jul;87(1):79-83
9233675
Trends Parasitol. 2002 Dec;18(12):528
12482534
J Med Microbiol. 1991 Jul;35(1):35-9
1906543
Parasitology. 2007 Mar;134(Pt 3):359-67
17052374