title: Lactobacillus colonization status in infants with urinary tract infection.
abstract: To explore the potential protective role of urogenital lactobacilli against urinary tract infection (UTI), lactobacillus cultures were performed on stool and urine specimens and periurethral/vaginal swabs of febrile infants who were suspected of having UTI. Those infants diagnosed with UTI based on the results of the suprapubic urine cultures were allocated to the UTI group (n = 60), and those who had a simple viral illness with negative urine cultures were allocated to the control group (n = 31). Lactobacilli were anaerobically cultured in lactobacillus-specific DifcoTM Rogosa SL agar for 48 h at 37 degrees C and then counted. The lactobacillus colony counts for the stool and urine specimens and periurethral swabs from the UTI group were significantly lower than those for the control group (P < 0.05). The geometric means of stool, periurethra, and urine lactobacilli in the UTI group were significantly lower than those in the control group (P < 0.05). The colony count of the vaginal lactobacillus demonstrated an equivocal difference between the UTI and control group. In conclusion, this is the first prospective case-control study to demonstrate reduced lactobacillus urogenital colonization in infants with UTI. Our results support the view that less urogenital lactobacillus colonization may be a risk factor for UTI in infants even though there is an unclear possibility that the UTI itself could be the cause of the lower lactobacillus colonies.
authors.affiliation: Department of Pediatrics, Hallym University, Kangnam Sacred Heart Hospital, 94-195 Yeongdeungpo-dong, Yeongdeungpo-gu, Seoul, 150-030, Korea.
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Shim
authors.forename: Seung Joo
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D016022:Case-Control Studies; D015169:Colony Count, Microbial; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D007778:Lactobacillus / Q000254:growth & development*; D008297:Male; D011446:Prospective Studies; D012307:Risk Factors; D014521:Urethra / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000175:diagnosis / Q000382:microbiology*; D014556:Urine / Q000382:microbiology*; D014621:Vagina / Q000382:microbiology*
references.citation: Can Med Assoc J. 1973 Apr 21;108(8):973-6
references.pmid: 4633489
references.citation: Clin Ther. 1992 Jan-Feb;14(1):11-6
references.pmid: 1576619
references.citation: Infect Immun. 1985 Aug;49(2):320-4
references.pmid: 3926645
references.citation: World J Urol. 2006 Feb;24(1):28-32
references.pmid: 16389539
references.citation: Biol Neonate. 2002 Aug;82(2):103-8
references.pmid: 12169832
references.citation: Appl Environ Microbiol. 1996 Jun;62(6):1958-63
references.pmid: 8787394
references.citation: J Urol. 1975 Feb;113(2):214-7
references.pmid: 803573
references.citation: Antimicrob Agents Chemother. 2001 Jun;45(6):1751-60
references.pmid: 11353622
references.citation: Infect Immun. 1989 Aug;57(8):2447-51
references.pmid: 2663724
references.citation: Roum Arch Microbiol Immunol. 2003 Jul-Dec;62(3-4):137-54
references.pmid: 16008140
references.citation: Acta Paediatr. 2004 Feb;93(2):164-8
references.pmid: 15046267
references.citation: Can J Microbiol. 1988 Mar;34(3):339-43
references.pmid: 3138016
references.citation: Pediatr Nephrol. 2007 Sep;22(9):1315-20
references.pmid: 17530295
references.citation: FEMS Immunol Med Microbiol. 2001 Feb;30(1):49-52
references.pmid: 11172991
references.citation: Br J Nutr. 2000 Feb;83(2):167-76
references.pmid: 10743496
references.citation: Immunol Cell Biol. 2000 Feb;78(1):67-73
references.pmid: 10651931
references.citation: J Infect Dis. 2001 Mar 1;183 Suppl 1:S77-80
references.pmid: 11171021
references.citation: J Infect Dis. 2001 Feb 1;183(3):485-91
references.pmid: 11133381
references.citation: J Urol. 1984 Mar;131(3):596-601
references.pmid: 6422061
references.citation: Clin Pediatr (Phila). 2004 Jan-Feb;43(1):95-8
references.pmid: 14968899
references.citation: J Infect Dis. 1998 Aug;178(2):446-50
references.pmid: 9697725
references.citation: J Clin Microbiol. 1980 Jun;11(6):654-9
references.pmid: 7000816
references.citation: J Pediatr. 2003 Dec;143(6):754-8
references.pmid: 14657823
references.citation: Antonie Van Leeuwenhoek. 2005 Jul;88(1):25-34
references.pmid: 15928974