Improvement of efficiency of genetic transformation for Dunaliella salina by glass beads method.
Dunaliella salina has been exploited as a new type of bioreactor due to its unique advantages. However, this bioreactor application was restricted for absence of a high-efficiency and stable transformation method at present. In the present study, the cells of D. salina were transformed by glass beads. The results of histochemical staining revealed that the GUS gene was successfully expressed in the positive transformants, and PCR and PCR-Southern blot analysis further demonstrated that the bar gene was integrated into the D. salina genome. Moreover, the three transformation methods, including glass beads, bombardment particle and electroporation, were compared for screening a high-efficiency transformation method for gene engineering of D. salina. The results showed that transformation efficiency of the glass beads was the highest, approximately 10(2) transformants/microg DNA. It is concluded that the established glass beads method has been demonstrated to be an optimal transformation way for D. salina.
Feng
Shuying
S
Laboratory for Cell Biology, The First Affiliated Hospital, Zhengzhou University Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, Henan, 450052, People's Republic of China.
Xue
Lexun
L
Liu
Hongtao
H
Lu
Pengju
P
D019149:Bioreactors; D018274:Electroporation; D056890:Eukaryota / Q000235:genetics*; D005818:Genetic Engineering; D005898:Glass; D008722:Methods; D014170:Transformation, Genetic
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