No relationship exists between PBP 2a amounts expressed in different MRSA strains obtained clinically and their beta-lactam MIC values.
After establishing a linear relationship between the amount of penicillin-binding protein (PBP) 2a and membrane proteins of methicillin-resistant Staphylococcus aureus (MRSA) COL by dot-blot analysis using an antibody against PBP 2a, we determined the PBP 2a quantities in membrane fractions prepared from 14 different MRSA cells. Methicillin-sensitive S. aureus ATCC 6538P was used as a quality control strain. The amounts of PBP 2a diverged among the strains, and no relationship to beta-lactam MIC values were observed in the corresponding strains.
Parvez
Md Anowar Khasru
MA
Department of Molecular Cell Biology and Medicine, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.
Shibata
Hirofumi
H
Nakano
Tatsuro
T
Niimi
Shingo
S
Fujii
Nobuo
N
Arakaki
Naokatu
N
Higuti
Tomihiko
T
D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D016106:Methicillin Resistance; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D047090:beta-Lactams / Q000008:administration & dosage / Q000494:pharmacology*
D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; D047090:beta-Lactams; C487789:mecA protein, Staphylococcus aureus