Lipid imaging with cluster time-of-flight secondary ion mass spectrometry.
This brief article provides an overview of the current state of the art in biological imaging mass spectrometry using cluster time-of-flight secondary ion mass spectrometry (TOF-SIMS). Recent and spectacular improvements in terms of sensitivity of TOF-SIMS imaging methods have allowed many biological applications to recently be successfully tested, such as mapping of lipid disorders in human muscles of a patient suffering from dystrophy, localization of surfactins after the swarming of bacteria on a surface, or lipid mapping over whole-body animal sections.
Brunelle
Alain
A
CNRS, Institut de Chimie des Substances Naturelles, Laboratoire de Spectrométrie de Masse, UPR 2301, Av. de la Terrasse, 91198, Gif-sur-Yvette, France. Alain.Brunelle@icsn.cnrs-gif.fr
Laprévote
Olivier
O
D000818:Animals; D001412:Bacillus subtilis / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*
D008055:Lipids