title: Chromatographic benefits of elevated temperature for the proteomic analysis of membrane proteins.
abstract: Integral membrane proteins (IMPs) perform crucial cellular functions and are the primary targets for most pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains and their intimate association with lipids make them difficult to handle. Numerous proteomic platforms that include LC separations have been reported for the high-throughput profiling of complex protein samples. However, there are still many challenges to overcome for proteomic analyses of IMPs, especially as compared to their soluble counterparts. In particular, considerations for the technical challenges associated with chromatographic separations are just beginning to be investigated. Here, we review the benefits of using elevated temperatures during LC for the proteomic analysis of complex membrane protein samples.
authors.affiliation: Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
authors.forename: Adele R
authors.initials: AR
authors.lastname: Blackler
authors.forename: Anna E
authors.initials: AE
authors.lastname: Speers
authors.forename: Christine C
authors.initials: CC
authors.lastname: Wu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D008565:Membrane Proteins / Q000032:analysis*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D008565:Membrane Proteins
references.citation: J Chromatogr A. 2005 Jun 24;1079(1-2):213-20
references.pmid: 16038307
references.citation: Expert Rev Proteomics. 2006 Dec;3(6):585-96
references.pmid: 17181473
references.citation: Mol Cell Proteomics. 2003 Nov;2(11):1234-43
references.pmid: 14506206
references.citation: J Chromatogr A. 1996 Jun 21;737(2):273-9
references.pmid: 8673256
references.citation: Mol Cell Proteomics. 2002 Jan;1(1):46-59
references.pmid: 12096140
references.citation: J Mol Biol. 1982 May 5;157(1):105-32
references.pmid: 7108955
references.citation: Nat Biotechnol. 2001 Mar;19(3):242-7
references.pmid: 11231557
references.citation: Drug Discov Today. 2005 Dec;10(23-24):1607-10
references.pmid: 16376820
references.citation: J Proteome Res. 2005 Sep-Oct;4(5):1522-37
references.pmid: 16212403
references.citation: J Chromatogr. 1987 Jul 10;398:335-9
references.pmid: 3654846
references.citation: J Chromatogr A. 2006 Sep 8;1126(1-2):6-49
references.pmid: 16908026
references.citation: J Chromatogr. 1988 Jul 8;445(1):71-86
references.pmid: 3215981
references.citation: J Sep Sci. 2007 Mar;30(5):658-68
references.pmid: 17461103
references.citation: J Chromatogr A. 2006 Feb 24;1107(1-2):139-51
references.pmid: 16412451
references.citation: Mol Biol Cell. 2004 Jun;15(6):2907-19
references.pmid: 15047867
references.citation: Biomed Chromatogr. 1993 Jan-Feb;7(1):15-9
references.pmid: 8381689
references.citation: J Chromatogr A. 2000 Feb 11;869(1-2):211-30
references.pmid: 10720238
references.citation: Pept Res. 1993 Sep-Oct;6(5):249-58
references.pmid: 8257801
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Mar 5;817(1):35-47
references.pmid: 15680787
references.citation: J Proteome Res. 2008 Jul;7(7):3028-34
references.pmid: 18537282
references.citation: J Chromatogr A. 2002 Aug 2;965(1-2):195-205
references.pmid: 12236525
references.citation: Chem Rev. 2007 Aug;107(8):3687-714
references.pmid: 17683161
references.citation: J Sep Sci. 2007 Feb;30(2):241-4
references.pmid: 17390618
references.citation: J Chromatogr A. 1996 Dec 20;756(1-2):21-39
references.pmid: 9008855
references.citation: Nat Biotechnol. 2006 Feb;24(2):191-7
references.pmid: 16415855
references.citation: Anal Chem. 2001 Dec 1;73(23):5683-90
references.pmid: 11774908
references.citation: Anal Chem. 2007 Jun 15;79(12):4613-20
references.pmid: 17500534
references.citation: Proc Int Conf Intell Syst Mol Biol. 1998;6:175-82
references.pmid: 9783223
references.citation: J Chromatogr A. 1994 Nov 25;686(1):31-43
references.pmid: 7849982
references.citation: J Proteome Res. 2005 Mar-Apr;4(2):349-57
references.pmid: 15822910
references.citation: J Chromatogr A. 1994 Nov 25;686(1):45-59
references.pmid: 7849983
references.citation: Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13207-12
references.pmid: 14576278
references.citation: J Sep Sci. 2004 Dec;27(17-18):1402-18
references.pmid: 15638149
references.citation: J Chromatogr A. 2004 Mar 19;1030(1-2):255-62
references.pmid: 15043277
references.citation: Mol Cell Proteomics. 2005 Apr;4(4):402-8
references.pmid: 15684408
references.citation: Expert Rev Proteomics. 2004 Aug;1(2):153-63
references.pmid: 15966810
references.citation: J Proteome Res. 2006 Oct;5(10):2701-10
references.pmid: 17022641
references.citation: J Proteome Res. 2006 Jun;5(6):1301-12
references.pmid: 16739982
references.citation: Nat Biotechnol. 1999 Jul;17(7):676-82
references.pmid: 10404161
references.citation: J Proteome Res. 2006 Aug;5(8):1860-5
references.pmid: 16889407
references.citation: Mol Biol Cell. 2006 Mar;17(3):1436-50
references.pmid: 16407407
references.citation: Nat Rev Drug Discov. 2002 Sep;1(9):727-30
references.pmid: 12209152
references.citation: Anal Biochem. 1984 Jul;140(1):223-35
references.pmid: 6486409
references.citation: J Chromatogr. 1982 Dec 3;252:241-54
references.pmid: 6304128
references.citation: Mol Cell Proteomics. 2006 Mar;5(3):444-53
references.pmid: 16291997
references.citation: Nat Biotechnol. 2003 Mar;21(3):262-7
references.pmid: 12610573