Serotype-specificity of recombinant fusion proteins containing domain III of dengue virus.
Here, the antigenic specificity of the recombinant fusion proteins containing aa 286-426 of the dengue envelope protein fused to P64k from Neisseria meningitidis and the cross-reactive antibody response induced in immunized mice and monkeys were evaluated. The anti-dengue mice antibodies showed a higher reactivity to the homologous recombinant proteins compared to the wide cross-reactivity observed by dot blot to the viral antigens. The immune response induced by the recombinant proteins in mice and monkeys, was highly serotype specific. The serotype-specificity associated with these recombinant proteins in addition to the high antigenicity, immunogenicity and protecting capacity suggest their advantages as possible vaccine candidates.
Izquierdo
Alienys
A
Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía, km 6 1/2 P.O. Box: 601 Marianao 13, Havana, Cuba.
Bernardo
Lidice
L
Martin
Jorge
J
Santana
Emidalys
E
Hermida
Lisset
L
Guillén
Gerardo
G
Guzmán
María G
MG
D000818:Animals; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D003715:Dengue / Q000276:immunology* / Q000821:virology; D053059:Dengue Vaccines / Q000737:chemistry / Q000276:immunology; D003716:Dengue Virus / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D008252:Macaca fascicularis; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis / Q000276:immunology; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology; D014709:Vero Cells
D053059:Dengue Vaccines; D000939:Epitopes; D011993:Recombinant Fusion Proteins