An immunocytochemical study of the endocrine cells in the stomach and duodenum of Zonotrichia capensis subtorquata (Passeriformes, Emberizidae).
The main purpose of this study was to evaluate the regional distribution pattern and relative frequency of some endocrine cells in the three portions of the gastrointestinal tract (GIT)--the proventriculus, gizzard and duodenum- of the rufous-collared sparrow (Zonotrichia capensis subtorquata), by immunohistochemical methods using six types of polyclonal antisera, specific for serotonin (5-HT), somatostatin (D cells), glucagon, motilin, polypeptide YY (PYY) and insulin. In the proventriculus, endocrine cells immunoreactive for all of these markers were observed. The somatostatin-immunoreactive cells were found with greater frequency, with the presence of cytoplasmic processes. In the gizzard, endocrine cells secreting somatostatin, 5-HT and PYY were detected, while those secreting glucagon and insulin were not. In the final part of the gizzard, endocrine cells secreting 5-HT were more frequent, and cells secreting somatostatin and insulin were not detected. All of the cell types studied were observed in the duodenum in different frequencies, except for cells immunoreactive for glucagon and insulin. The somatostatin-positive (D cells) were the most numerous, being more prevalent in the intestinal glands. The other endocrine cells were identified in smaller numbers, some of them located in the intestinal villi and Lieberkuhn glands. The finding of these cell types in the duodenum confirms their preferential location in the final portions of the principal segments of the digestive system and suggests control by feedback of its functions. In conclusion, some interesting distributional patterns of gastrointestinal endocrine cells were found in this species of sparrow.
Mendes
Rosa Maria Marcos
RM
Laboratório de Histologia e Embriologia, Animal Biology Department, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Rod. BR 465, Km 07, CEP 23.890-000, Seropédica, Brazil. r.mendes@superig.com.br
do Nascimento
Aparecida Alves
AA
Dos Santos
Clarice Machado
CM
Cardoso
Tânia Regina Dantas
TR
Pinheiro
Nadja Lima
NL
Sales
Armando
A
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004386:Duodenum / Q000737:chemistry / Q000166:cytology*; D055098:Endocrine Cells / Q000737:chemistry / Q000166:cytology*; D005895:Gizzard, Avian / Q000737:chemistry / Q000166:cytology; D005934:Glucagon / Q000032:analysis; D007150:Immunohistochemistry; D007328:Insulin / Q000032:analysis; D009037:Motilin / Q000032:analysis; D046109:Passeriformes; D019894:Peptide YY / Q000032:analysis; D012701:Serotonin / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D013270:Stomach / Q000737:chemistry / Q000166:cytology*
D015415:Biomarkers; D007328:Insulin; D019894:Peptide YY; D012701:Serotonin; D013004:Somatostatin; D009037:Motilin; D005934:Glucagon