title: Pulmonary immune responses induced in BALB/c mice by Paracoccidioides brasiliensis conidia.
abstract: Knowledge concerning the host-Paracoccidioides brasiliensis interactions is abundant. Yet, most of the experimental studies have used yeast cells to prepare the corresponding inoculum. As these cells do not represent the naturally infecting propagules, the corresponding experiments by-pass the earlier stages of such interactions. Studies done in patients, who also harbour yeast cells, suffer from the same bias. The review presented below focuses on the immune responses of BALB/c mice infected with conidia obtained from P. brasiliensis mycelial form cultures, the fungal stage most probably existing in nature. As such, the corresponding experiments would copy the onset and course of the human infection. A large number of experimental studies done by the CIB Medical and Experimental Mycology Unit in a period of almost 25 years have been revised and extracted so as to present a comprehensive record on the immune responses induced when mice are infected intranasally with the conidia. The establishment of this mouse model has permitted the analysis of the immune responses taking place during the early and late stages post-challenge. This unique model has made possible to characterize the course of the experimental disease including the inflammatory reaction, the expression of cytokines and of the various molecules associated to these responses, all of which lead to granuloma formation. The latter structure serves as a nest for the development of fibrosis. Thus, we have also obtained a glimpse on the complexity that accompanies the fibrosis, the most common sequelae of paracoccidioidomycosis. Additionally, a concerted effort has been made to appraise the whole gamut of immune factors and related molecules that directly or indirectly, contribute to shape the pathogenesis of this Latin American mycosis.
authors.affiliation: Medical and Experimental Mycology Group, Corporación para Investigaciones Biológicas, CIB, Medellin, Colombia.
authors.forename: Angel
authors.initials: A
authors.lastname: González
authors.forename: Angela
authors.initials: A
authors.lastname: Restrepo
authors.forename: Luz Elena
authors.initials: LE
authors.lastname: Cano
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D008168:Lung / Q000276:immunology* / Q000382:microbiology; D008172:Lung Diseases, Fungal / Q000276:immunology* / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D025282:Mycelium / Q000276:immunology* / Q000472:pathogenicity; D010228:Paracoccidioides / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D010229:Paracoccidioidomycosis / Q000276:immunology* / Q000382:microbiology
references.citation: J Leukoc Biol. 1996 Apr;59(4):526-33
references.pmid: 8613700
references.citation: Am J Trop Med Hyg. 2004 Dec;71(6):828-30
references.pmid: 15642978
references.citation: Infect Immun. 2000 Oct;68(10):5668-72
references.pmid: 10992469
references.citation: Sabouraudia. 1981 Sep;19(3):165-78
references.pmid: 7292205
references.citation: J Clin Invest. 1987 Feb;79(2):319-26
references.pmid: 3543052
references.citation: J Immunol. 2000 Nov 15;165(10):5760-6
references.pmid: 11067934
references.citation: Curr Opin Cell Biol. 1994 Oct;6(5):752-8
references.pmid: 7833055
references.citation: Infect Immun. 2006 Jan;74(1):382-9
references.pmid: 16368993
references.citation: Pathol Microbiol (Basel). 1963;26:29-43
references.pmid: 14015551
references.citation: Biochem Biophys Res Commun. 1989 Jun 15;161(2):420-6
references.pmid: 2735902
references.citation: Clin Infect Dis. 1995 Oct;21 Suppl 2:S162-5
references.pmid: 8845445
references.citation: Biochem Biophys Res Commun. 1988 Nov 30;157(1):87-94
references.pmid: 3196352
references.citation: J Electron Microsc (Tokyo). 2001;50(1):41-9
references.pmid: 11291959
references.citation: Infect Immun. 2005 Apr;73(4):2486-95
references.pmid: 15784595
references.citation: J Immunol. 1987 Jan 15;138(2):550-65
references.pmid: 2432129
references.citation: J Immunol. 1998 Sep 15;161(6):3056-63
references.pmid: 9743371
references.citation: Infect Immun. 1977 Sep;17(3):580-5
references.pmid: 332628
references.citation: Med Mycol. 2005 Nov;43(7):637-45
references.pmid: 16396249
references.citation: Infect Immun. 1977 Jun;16(3):974-82
references.pmid: 561033
references.citation: J Med Microbiol. 1999 Aug;48(8):721-730
references.pmid: 10450995
references.citation: Int J Immunopharmacol. 1988;10(8):945-52
references.pmid: 3145925
references.citation: Rev Inst Med Trop Sao Paulo. 2007 Jan-Feb;49(1):11-6
references.pmid: 17384814
references.citation: Br J Haematol. 2005 Jun;129(5):583-96
references.pmid: 15916680
references.citation: Am J Trop Med Hyg. 1987 May;36(3):603-8
references.pmid: 3555139
references.citation: J Immunol. 2002 May 1;168(9):4593-600
references.pmid: 11971007
references.citation: Infect Immun. 2000 May;68(5):2546-52
references.pmid: 10768942
references.citation: Eur J Immunol. 1988 Oct;18(10):1587-92
references.pmid: 3142779
references.citation: Science. 1987 Jan 23;235(4787):473-6
references.pmid: 2432665
references.citation: Med Mycol. 1998 Oct;36(5):345-8
references.pmid: 10075506
references.citation: Clin Microbiol Rev. 1993 Apr;6(2):89-117
references.pmid: 8472249