Genes involved in the benzoate catabolic pathway in Acinetobacter calcoaceticus PHEA-2.
A putative benM gene encoding a LysR-type regulator located upstream from the benA gene was found in Acinetobacter calcoaceticus PHEA-2. Disruption of benM or benA destroyed the ability of PHEA-2 to utilize benzoate. The benM mutant was used to construct a genomic library for isolation of the complete gene cluster responsible for benzoate degradation. Sequence analysis showed that the cluster has three putative operons: benM, benABCDE, and benKP. Unlike many well-characterized benzoate-degrading bacteria, muconate is unable to induce in vivo transcription of the PHEA-2 ben cluster. Reverse transcriptase-polymerase chain reaction (RT-PCR) results showed that the benABCDE operon is activated by the BenM protein in the presence of benzoate. Moreover, a gel-retardation assay demonstrated that BenM binds to the promotor region of the benA gene. The activities of catechol 1,2-dioxygenase (C12O) and catechol 2,3-dioxygenase (C23O) showed that PHEA-2 converted benzoate to catechol for further degradation, possibly via an ortho-cleavage pathway.
Zhan
Yuhua
Y
College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China.
Yu
Haiying
H
Yan
Yongliang
Y
Chen
Ming
M
Lu
Wei
W
Li
Shuying
S
Peng
Zixin
Z
Zhang
Wei
W
Ping
Shuzhen
S
Wang
Jin
J
Lin
Min
M
D016954:Acinetobacter calcoaceticus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D001565:Benzoates / Q000378:metabolism*; D050548:Catechol 1,2-Dioxygenase / Q000378:metabolism; D050557:Catechol 2,3-Dioxygenase / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D017353:Gene Deletion; D020869:Gene Expression Profiling; D023061:Gene Order; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects
D001426:Bacterial Proteins; D001565:Benzoates; D004269:DNA, Bacterial; C005939:muconic acid; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D013011:Sorbic Acid
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