title: Whole blood analysis of leukocyte-platelet aggregates.
abstract: In inflammatory and thrombotic syndromes, platelets aggregate with circulating leukocytes, especially monocytes and neutrophils. The platelet binding is initiated primarily through platelet surface expression of P-selectin (CD62P) following activation-dependent degranulation. The levels of P-selectin involved can be low enough to make direct measurement difficult, but detection of leukocyte-platelet aggregates is relatively simply by whole-blood flow cytometry. Light scatter and at least one leukocyte-specific antibody are used to gate the desired population, and the presence of associated platelets is detected by immunostaining for abundant platelet-specific markers.
authors.affiliation: University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Marc R
authors.initials: MR
authors.lastname: Barnard
authors.forename: Lori A
authors.initials: LA
authors.lastname: Krueger
authors.forename: A L
authors.initials: AL
authors.lastname: Frelinger
authors.forename: Mark I
authors.initials: MI
authors.lastname: Furman
authors.forename: Alan D
authors.initials: AD
authors.lastname: Michelson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001769:Blood; D001792:Blood Platelets / Q000166:cytology* / Q000276:immunology; D002448:Cell Adhesion; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology; D008722:Methods
chemical_list: D000911:Antibodies, Monoclonal