title: The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens.
abstract: The Seeplex TB Detection-2 assay (Rockville, MD) is a nested endpoint PCR for the Mycobacterium tuberculosis complex (MTBC) targets IS6110 and MPB64 that utilizes dual priming oligonucleotide technology. When used to detect the presence of MTBC DNA in formalin-fixed paraffin-embedded tissue specimens, the sensitivity and specificity of this assay is equivalent to a labor-intensive traditional endpoint PCR assay and is more sensitive than a commercial real-time PCR assay.
authors.affiliation: Public Health Laboratories Branch, Ministry of Health and Long-Term Care, Toronto, Ontario, Canada. steven.drews@ontario.ca
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Drews
authors.forename: AliReza
authors.initials: A
authors.lastname: Eshaghi
authors.forename: Daria
authors.initials: D
authors.lastname: Pyskir
authors.forename: Pam
authors.initials: P
authors.lastname: Chedore
authors.forename: Ernesto
authors.initials: E
authors.lastname: Lombos
authors.forename: George
authors.initials: G
authors.lastname: Broukhanski
authors.forename: Rachel
authors.initials: R
authors.lastname: Higgins
authors.forename: David N
authors.initials: DN
authors.lastname: Fisman
authors.forename: Joanne
authors.initials: J
authors.lastname: Blair
authors.forename: Frances
authors.initials: F
authors.lastname: Jamieson
references.citation: J Clin Microbiol. 1993 Feb;31(2):406-9
references.pmid: 8381814
references.citation: Nucleic Acids Res. 2007;35(6):e40
references.pmid: 17287288
references.citation: Int J Tuberc Lung Dis. 2006 Mar;10(3):340-2
references.pmid: 16562717
references.citation: J Clin Microbiol. 1998 Jun;36(6):1512-7
references.pmid: 9620369
references.citation: J Forensic Sci. 1998 Sep;43(5):1024-30
references.pmid: 9729819
references.citation: APMIS. 2005 Sep;113(9):586-93
references.pmid: 16218933
references.citation: Indian J Med Res. 2005 Aug;122(2):165-70
references.pmid: 16177475
references.citation: J Microbiol Methods. 2006 Jul;66(1):177-80
references.pmid: 16427712