title: Contrast enhancement in light microscopy.
abstract: The optical microscope is a fundamental component of an image cytometry system. Electronic imaging has both greatly enhanced the capabilities of light microscopes and placed ever-increasing demands on their optical performance. This unit covers the basic concepts of light microscopy, including Köhler illumination, resolution, contrast, and numerical aperture, and reviews the many types of instruments and techniques for contrast enhancement.
authors.affiliation: Carl Zeiss, Inc., Thornwood, New York, USA.
authors.forename: H E
authors.initials: HE
authors.lastname: Keller
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000494:pharmacology; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D008027:Light; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000379:methods; D008859:Microscopy, Polarization / Q000379:methods; D010825:Physics / Q000379:methods
chemical_list: D003287:Contrast Media