WU and KI polyomavirus present in the respiratory tract of children, but not in immunocompetent adults.
BACKGROUND
Recently, two new polyomaviruses (PyV), termed WUPyV and KIPyV, were identified in respiratory tract specimens from children with acute respiratory tract infections (ARTIs). However, their roles in the disease have not been determined.


OBJECTIVE
To determine the prevalence of WUPyV and KIPyV in the Chinese population suffering from ARTIs in Beijing, China, and to examine their possible role in causing disease.


METHODS
Nasopharyngeal aspirates, nasal swabs and throat swabs were collected from 415 children and 297 immunocompetent adults with lower ARTIs (LARTIs). The specimens were screened by polymerase chain reaction for the presence of WUPyV, KIPyV, and other common respiratory pathogens.


RESULTS
Although none of the adults sampled were positive for either virus, WUPyV in 10 (2.4%) children and KIPyV was detected in 2 (0.5%) of the children sampled, respectively. Eleven of the positive cases were co-detected with either rhinovirus (6/11), respiratory syncytial virus (4/11), parainfluenzavirus virus (3/11) or Mycoplasma pneumoniae (2/11). Phylogenetic analysis of the WUPyV and KIPyV isolates showed that the nucleotide sequences were homologous to those of previously reported strains.


CONCLUSIONS
The presence of WUPyV and KIPyV in samples from children but not from immunocompetent adults suffering from LARTIs suggests that these viruses primarily infect the young population. Co-detection of additional respiratory pathogens in most of the specimens containing either WUPyV or KIPyV suggests that these viruses do not cause disease independently.
Ren
Lili
L
Dr. Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux and State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS), Beijing 100730, P R China.
Gonzalez
Richard
R
Xie
Zhengde
Z
Zhang
Jing
J
Liu
Chunyan
C
Li
Jianguo
J
Li
Yongjun
Y
Wang
Zhong
Z
Kong
Xiaohui
X
Yao
Yuan
Y
Hu
Yinghui
Y
Qian
Suyun
S
Geng
Rong
R
Yang
Yan
Y
Vernet
Guy
G
Paranhos-Baccalà
Gláucia
G
Jin
Qi
Q
Shen
Kunling
K
Wang
Jianwei
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009297:Nasal Mucosa / Q000821:virology; D009305:Nasopharynx / Q000821:virology; D010614:Pharynx / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011120:Polyomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA
D004279:DNA, Viral
Emerg Infect Dis. 2007 Dec;13(12):1939-41
18258053
Emerg Infect Dis. 2007 Nov;13(11):1766-8
18217567
J Virol. 2007 Apr;81(8):4130-6
17287263
Emerg Infect Dis. 2007 Dec;13(12):1936-8
18258052
J Med Virol. 2004 Mar;72(3):484-95
14748074
Emerg Infect Dis. 2006 Aug;12(8):1254-6
16965708
J Clin Microbiol. 1990 Dec;28(12):2659-67
2279998
J Clin Virol. 2008 Feb;41(2):63-8
18083616
J Virol. 2005 Jan;79(2):884-95
15613317
J Med Virol. 2003 Jan;69(1):132-44
12436489
Emerg Infect Dis. 2003 Jun;9(6):628-33
12781000
PLoS Pathog. 2007 May 4;3(5):e64
17480120
Emerg Infect Dis. 2008 Apr;14(4):680-1
18394299
Virus Res. 2008 Aug;135(2):230-6
18468712
Trop Med Int Health. 2002 Apr;7(4):317-21
11952947
J Clin Virol. 2007 Dec;40(4):307-11
17997354