Isolation and partial characterisation of four novel plasma lectins from the American lobster Homarus americanus.
Although numerous haemolymph-derived crustacean lectins are described, few have been reported for the American lobster Homarus americanus. In the present study, affinity chromatography was used to isolate and partially describe the carbohydrate affinity of four new lectins from H. americanus plasma. HaMBP and HaDNABP were homodimers of approximately 30 kDa subunits which bound to mannan- and DNA-agarose columns, respectively. These proteins had partially overlapping elution profiles, and both shared and unique amino acid sequences and fragmentation patterns after trypsin digestion. A third homodimer of approximately 29 kDa subunits eluted with HaMBP and HaDNABP under certain conditions. HaNBP occurred as a monomer and dimer of approximately 40 kDa subunits and was recovered in relatively large quantities from mannan-agarose with N-acetylated sugars. Transmission electron microscopy revealed HaNBP to be a linear protein composed of multiple globular subunits.
Battison
Andrea L
AL
AVC Lobster Science Centre, University of Prince Edward Island, Charlottetown, PE, Canada. abattison@upei.ca
Summerfield
Rachael L
RL
D000818:Animals; D037102:Lectins / Q000097:blood* / Q000302:isolation & purification* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008121:Nephropidae / Q000378:metabolism*
D037102:Lectins