Differentiation of mouse embryonic stem cells into blood.
Embryonic stem (ES) cells can be maintained as pluripotent stem cells or induced to differentiate into many different somatic cell types. As ES-derived somatic cells can potentially be used for cell transplantation or cell-based therapy, ES cells have gained much scientific and general public attention. Successful derivation of blood from ES cells for tissue engineering will require a comprehensive understanding of inductive signals and downstream effectors involved in blood lineage development. Ideally, directed differentiation of ES cells into blood and isolation of pure hematopoietic progenitors will enhance our ability to utilize ES-derived blood cells for future clinical applications. The protocols provided in this unit describe methods of maintaining and differentiating mouse ES cells as well as identifying and isolating hematopoietic progenitors by utilizing flow cytometry and progenitor assays.
Ma
Yunglin D
YD
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri, USA.
Lugus
Jesse J
JJ
Park
Changwon
C
Choi
Kyunghee
K
D000818:Animals; D001773:Blood Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D053595:Embryonic Stem Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology; D051379:Mice; D044967:Serum
D016895:Culture Media, Serum-Free
Nature. 1995 Feb 2;373(6513):432-4
7830794
Mol Cell Biol. 1993 Jan;13(1):473-86
8417345
Blood. 2005 Jan 1;105(1):111-4
15231577
Development. 1998 May;125(9):1747-57
9521912
Development. 1991 Feb;111(2):259-67
1893864
Nature. 1991 Jan 17;349(6306):257-60
1987478
Development. 2002 Dec;129(23):5511-20
12403720
Development. 2007 Jan;134(2):393-405
17166922
Cell. 1996 Jan 26;84(2):321-30
8565077
Curr Top Dev Biol. 2005;66:1-36
15797450
Nature. 1997 Apr 3;386(6624):488-93
9087406
Development. 1998 Feb;125(4):725-32
9435292
Eur J Immunol. 1988 Jan;18(1):97-104
2831066
Science. 1994 Aug 19;265(5175):1098-101
8066449
Nature. 1994 Sep 15;371(6494):221-6
8078582
Development. 2004 Jun;131(11):2749-62
15148304
Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3444-9
8622955
Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6027-32
15075392