title: Enhanced in vitro cell activity on silicon-doped vaterite/poly(lactic acid) composites.
abstract: A biodegradable composite with silicon-species releasability was prepared using poly(l-lactic acid) (PLLA) and silicon-doped vaterite (SiV) particles. SiV with particle diameters of approximately 1 mum was prepared using aminopropyltriethoxysilane (APTES) as the silicon species by a carbonation process and then mixed with PLLA in methylene chloride according to a SiV to PLLA weight ratio of 1:2, resulting in the preparation of composite slurry. A composite film was prepared by dipping a cover glass in the slurry. The composite films were incubated in a culture medium for 7 days and the silicon concentration of the medium was measured to estimate the species releasability of the composites. A trace amount of silicon species was continuously released from the composites for 7 days, the amount depending on the content of APTES in SiV. On the composite releasing silicon species, mouse osteoblast-like cells (MC3T3-E1 cells) were significantly stimulated to proliferate and differentiate in comparison with those on a composite containing no silicon species. The proliferation of the cells on the composites releasing larger amounts of silicon species (0.51mgl(-1)day(-1)) was higher than that on the composites releasing smaller amount of the species (0.21mgl(-1)day(-1)). The silicon species in the composites were effective in enhancing the cellular functions. The composites were expected to be useful as a scaffold material for bone tissue engineering.
authors.affiliation: Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan. obata.akiko@nitech.ac.jp
authors.forename: Akiko
authors.initials: A
authors.lastname: Obata
authors.forename: Shingo
authors.initials: S
authors.lastname: Tokuda
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kasuga
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002119:Calcium Carbonate / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000737:chemistry*; D008752:Methylene Chloride / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D008970:Molecular Weight; D010006:Osteoblasts / Q000378:metabolism; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011437:Propylamines; D012821:Silanes / Q000494:pharmacology; D012825:Silicon / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D011437:Propylamines; D012821:Silanes; D019344:Lactic Acid; C033616:poly(lactide); D008752:Methylene Chloride; D002119:Calcium Carbonate; C477625:amino-propyl-triethoxysilane; D012825:Silicon