Diagnosis and treatment of mycoplasma-contaminated cell cultures.
Mycoplasma contamination is a serious and frequent problem in the culture laboratory. Although mycoplasma contamination may be suspected by the failure of cells to thrive, the formal diagnosis rests on the detection of adenosine phosphorylase secretion by infected cell lines. This appendix describes how to test for mycoplasma contamination, and also presents methods for antibiotic treatment of infected cultures.
Harlin
Helena
H
University of Chicago, Chicago, Illinois, USA.
Gajewski
Thomas F
TF
D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002939:Ciprofloxacin / Q000494:pharmacology*; D004224:Diterpenes / Q000494:pharmacology; D008911:Minocycline / Q000494:pharmacology; D009174:Mycoplasma / Q000187:drug effects*; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000188:drug therapy*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D059005:Topoisomerase II Inhibitors
D004224:Diterpenes; D011500:Protein Synthesis Inhibitors; D059005:Topoisomerase II Inhibitors; C074450:BM-cyclin; D002939:Ciprofloxacin; D008911:Minocycline