Fgf16(IRESCre) mice: a tool to inactivate genes expressed in inner ear cristae and spiral prominence epithelium.
Fibroblast growth factors play important roles in inner ear development. Previous studies showed that mouse Fgf16 is expressed asymmetrically during the otic cup and vesicle stages of development, suggesting roles in regulating or responding to anteroposterior axial cues. Here, we studied otic Fgf16 expression throughout embryonic development and found transcripts in the developing cristae and in a few cells in the lateral wall of the cochlear duct. To determine the otic function of Fgf16 and to follow the fate of Fgf16-expressing cells, we generated an Fgf16(IRESCre) allele. We show that Fgf16 does not have a unique role in inner ear development and that the Fgf16 lineage is found throughout the three cristae, in portions of the semicircular canal ducts, and in the cochlear spiral prominence epithelial cells. This strain will be useful for gene ablations in these tissues.
Hatch
Ekaterina P
EP
Department of Human Genetics, University of Utah, Salt Lake City, Utah, USA.
Urness
Lisa D
LD
Mansour
Suzanne L
SL
D000818:Animals; D019521:Body Patterning; D019070:Cell Lineage / Q000502:physiology; D003053:Cochlear Duct / Q000196:embryology / Q000378:metabolism; D007758:Ear, Inner / Q000196:embryology* / Q000378:metabolism; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D012665:Semicircular Canals / Q000196:embryology / Q000378:metabolism
C484686:Fgf16 protein, mouse; D005346:Fibroblast Growth Factors
J Neurosci. 2008 Jun 4;28(23):5991-9
18524904
Dev Dyn. 2003 Jul;227(3):379-86
12815623
Development. 2004 Sep;131(17):4201-11
15280215
J Neurosci. 1998 May 1;18(9):3327-35
9547240
Dev Biol. 2000 Nov 15;227(2):256-70
11071753
PLoS Genet. 2008 Apr 11;4(4):e1000050
18404215
Dev Biol. 1999 Dec 1;216(1):369-81
10588886
Development. 1993 Jan;117(1):13-28
8223243
Nat Genet. 1999 Jan;21(1):70-1
9916792
Biochem Biophys Res Commun. 1998 Feb 4;243(1):148-52
9473496
Development. 2007 Jan;134(1):167-76
17164422
Hear Res. 1999 Apr;130(1-2):94-107
10320101
J Biol Chem. 2006 Jun 9;281(23):15694-700
16597617
Nat Genet. 2000 Jun;25(2):139-40
10835623
Dev Dyn. 2003 Oct;228(2):267-72
14517998
Dev Biol. 2002 Sep 15;249(2):237-54
12221004
BMC Med. 2004 Aug 20;2:30
15320950
Dev Biol. 2004 Sep 15;273(2):350-60
15328018
Dev Dyn. 2005 May;233(1):177-87
15765517
Biochem Biophys Res Commun. 2006 Aug 18;347(1):340-6
16815307
Genesis. 2004 Jul;39(3):206-11
15282747
Development. 2000 Jan;127(1):45-54
10654599
Genesis. 2004 Apr;38(4):195-9
15083520
Development. 2007 Oct;134(20):3615-25
17855431
Dev Dyn. 2003 Jun;227(2):203-15
12761848
Development. 1998 Jan;125(1):11-20
9389659
Development. 2006 Feb;133(4):581-90
16407399
Hear Res. 1997 Dec;114(1-2):53-61
9447918
Int J Dev Biol. 2007;51(6-7):521-33
17891714
Development. 1989 Jan;105(1):131-6
2680421
Genesis. 2005 Nov;43(3):129-35
16267821
Dev Dyn. 2008 Jan;237(1):163-9
18058922
Dev Biol. 1990 Feb;137(2):243-57
2303163
Nat Genet. 2002 Jan;30(1):31-9
11753384
Dev Dyn. 2006 Aug;235(8):2276-81
16786592
Dev Cell. 2005 Jan;8(1):85-95
15621532
Genesis. 2007 Oct;45(10):618-24
17941042
J Neurosci. 2000 Aug 15;20(16):6125-34
10934262