[Effect of Bio-Oss loading with rAAV-BMP7 on regeneration of bone defects around dental implant].
OBJECTIVE
To evaluate in vivo gene delivery of Bio-Oss coated with adeno-associated virus-mediated human bone morphogenetic protein 7 (rAAV-BMP7/Bio-Oss) for bone regeneration around dental implants.


METHODS
In vitro rAAV-BMP7 were constructed and compounded with Bio-Oss. In 6 male New Zealand rabbits, two hydroxyapatite (HA) coated titanium dental implants were placed respectively to each animal in the bilateral tibia metaphysis. Before implantation, a standardized gap (8 mm in width, 4 mm in depth) was created between the implant surface and the surrounding bone walls. Rabbits were randomly divided into three groups (group A, B, C). Gaps of group A were filled with rAAV-BMP7/Bio-Oss (n = 4), gaps of group B were filled with Bio-Oss alone (n = 4), and gaps of group C were filled with nothing (n = 4). The rabbits were sacrificed at 4 and 8 weeks respectively, and the sclerous tissue slices obtained, then histology and histomorphometric analysis were conducted.


RESULTS
Histological and histomorphometric analysis revealed an enlarged bone-forming area in the bone defects of group A and B at 4 and 8 weeks after implantation. Greater bone-implant contact was achieved with rAAV-BMP7/Bio-Oss than with Bio-Oss alone and this difference was statistically significant (P < 0.05).


CONCLUSIONS
rAAV-BMP7/Bio-Oss can induce a stronger peri-implant bone reaction and larger new bone formation than Bio-Oss alone in the treatment of bone defects adjacent to titanium dental implants.
Song
Ke
K
Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Du
Jian-ming
JM
Luo
Ren-hui
RH
Cao
Ying-guang
YG
D000818:Animals; D055419:Bone Morphogenetic Protein 7; D001861:Bone Regeneration; D018786:Bone Substitutes; D001842:Bone and Bones; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D017886:Durapatite; D006801:Humans; D008297:Male; D008903:Minerals; D011817:Rabbits; D014025:Titanium
C525819:BMP7 protein, human; C077540:Bio-Oss; D055419:Bone Morphogenetic Protein 7; D018786:Bone Substitutes; D015921:Dental Implants; D008903:Minerals; D017886:Durapatite; D014025:Titanium