Ascidian sperm activating and attracting factor: importance of sulfate groups for the activities and implication of its putative receptor.
The sperm activating and attracting factor (SAAF) from the eggs of the ascidian Cionaintestinalis was identified as the sulfated polyhydroxysterol,3alpha,4beta,7alpha,26-tetrahydroxy-5alpha-cholestane-3,26-disulfate. We present a functional analysis of SAAF derivatives that reveals the roles of the various SAAF functional groups. Optical isomerism does not affect SAAF activities. Hydrolysis on one side, i.e. at the sulfate groups of SAAF, decreases the sperm-activating and sperm-attracting activities, while hydrolysis on both sides resulted in the loss of both activities. Biotinylated-SAAF lost its sperm-activating ability, but retained sperm-binding and chemotactic abilities. Thus, the sulfate groups of SAAF are responsible for these activities.
Yoshida
Manabu
M
Misaki Marine Biological Station, Graduate School of Science, University of Tokyo, 1024 Koajiro, Misaki, Miura 238-0225, Japan. yoshida@mmbs.s.u-tokyo.ac.jp
Shiba
Kogiku
K
Yoshida
Kaoru
K
Tsuchikawa
Hiroshi
H
Ootou
Ouichirou
O
Oishi
Toru
T
Murata
Michio
M
D000818:Animals; D002633:Chemotaxis; D002777:Cholestanols / Q000737:chemistry* / Q000494:pharmacology*; D002938:Ciona intestinalis / Q000166:cytology / Q000502:physiology*; D003460:Crystallization; D008297:Male; D015394:Molecular Structure; D011791:Quartz / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000819:agonists / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013463:Sulfuric Acid Esters / Q000737:chemistry* / Q000494:pharmacology*
C533418:3,4,7,26-tetrahydroxycholestane-3,26-disulfate; D002777:Cholestanols; D011956:Receptors, Cell Surface; D013463:Sulfuric Acid Esters; C035027:egg surface sperm receptor; D011791:Quartz