Real-time polymerase chain reaction to follow the response of muscle to training.
The adaptive response of muscle to changes in activity or loading can take many weeks. Changes in the levels of RNA within a muscle fiber can give an early indication of the nature of the response of that fiber to changes in activity or loading. We have designed a new primer set for quantitative polymerase chain reaction (PCR) that will allow us to follow these early transcriptional changes in rat muscle, and have shown that analysis can be performed by standard techniques on as little as 5 mg of muscle, an amount that can be obtained by needle biopsy.
Moore
Lauren M
LM
Department of Human Anatomy and Cell Biology, School of Biomedical Sciences, the University of Liverpool, Liverpool, UK.
Fisher
Andrew G
AG
Coulson
Judy M
JM
Salmons
Stanley
S
Jarvis
Jonathan C
JC
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000235:genetics; D055435:Myostatin; D005082:Physical Exertion; D020033:Protein Isoforms / Q000235:genetics; D012313:RNA / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics
D017931:DNA Primers; C525913:Mstn protein, rat; D055435:Myostatin; D020033:Protein Isoforms; D016212:Transforming Growth Factor beta; D012313:RNA; D007334:Insulin-Like Growth Factor I; D018995:Myosin Heavy Chains