title: Thermodynamic studies of interactions of calf spleen PNP with acyclic phosphonate inhibitors.
abstract: The Gibbs binding energy and entropy/enthalpy contributions to the interaction of calf spleen purine nucleoside phosphorylase (PNP) with the novel multisubstrate analogue DFPP-DG, as well as with DFPP-G and (S)-PMP-DAP were determined by fluorescence and calorimetric studies. Results were compared with findings for guanine - a natural reaction product and inhibitor.
authors.affiliation: Department of Biophysics, Institute of Experimental Physics, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Breer
authors.forename: Beata
authors.initials: B
authors.lastname: Wielgus-Kutrowska
authors.forename: Mariko
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Sadao
authors.initials: S
authors.lastname: Hikishima
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Yokomatsu
authors.forename: Roman H
authors.initials: RH
authors.lastname: Szczepanowski
authors.forename: Matthias
authors.initials: M
authors.lastname: Bochtler
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Girstun
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Starón
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bzowska
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry*; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D063065:Organophosphonates / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D011683:Purine-Nucleoside Phosphorylase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D013154:Spleen / Q000201:enzymology; D013816:Thermodynamics
chemical_list: C090125:9-(2-phosphonylmethoxypropyl)-2,6-diaminopurine; C528379:9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine; C509941:9-(5',5'-difluoro-5'-phosphonopentyl)guanine; D004791:Enzyme Inhibitors; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D006147:Guanine; D011683:Purine-Nucleoside Phosphorylase; D000225:Adenine