Laser-induced fluorescence imaging of subsurface tissue structures with a volume holographic spatial-spectral imaging system.
A three-dimensional imaging system incorporating multiplexed holographic gratings to visualize fluorescence tissue structures is presented. Holographic gratings formed in volume recording materials such as a phenanthrenquinone poly(methyl methacrylate) photopolymer have narrowband angular and spectral transmittance filtering properties that enable obtaining spatial-spectral information within an object. We demonstrate this imaging system's ability to obtain multiple depth-resolved fluorescence images simultaneously.
Luo
Yuan
Y
Department of Electrical and Computing Engineering, University of Arizona, Tucson, AZ 85721, USA. luo@email.arizona.edu
Gelsinger-Austin
Paul J
PJ
Watson
Jonathan M
JM
Barbastathis
George
G
Barton
Jennifer K
JK
Kostuk
Raymond K
RK
D000165:Acridine Orange; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D003106:Colon / Q000166:cytology; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods; D005456:Fluorescent Dyes; D006696:Holography / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D051379:Mice
D005456:Fluorescent Dyes; D000165:Acridine Orange