[Heart tissue from embryonic stem cells].
Embryonic stem cells can give rise to all somatic cells, making them an attractive cell source for tissue engineering applications. The propensity of cells to form tissue-like structures in a culture dish has been well documented. We and others made use of this intrinsic property to generate bioartificial heart muscle. First proof-of-concept studies involved immature heart cells mainly from fetal chicken, neonatal rats and mice. They eventually provided evidence that force-generating heart muscle can be engineered in vitro. Recently, the focus shifted to the application of stem cells to eventually enable the generation of human heart muscle and reach following long-term goals: (1) development of a simplified in vitro model of heart muscle development; (2) generation of a human test-bed for drug screening and development; (3) allocation of surrogate heart tissue to myocardial repair applications. This overview will provide the background for cell-based myocardial repair, introduce the main myocardial tissue engineering concepts, discuss the use of embryonic and non-embryonic stem cells, and lays out the potential direct and indirect therapeutic use of human tissue engineered myocardium.
Zimmermann
W-H
WH
Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, BRD. w.zimmermann@uke.uni-hamburg.de
D000826:Animal Testing Alternatives; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D033041:Embryo Research; D053595:Embryonic Stem Cells / Q000637:transplantation*; D006801:Humans; D009206:Myocardium / Q000166:cytology*; D012038:Regeneration / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D016896:Treatment Outcome