Altered expression of UVB-induced cytokines in human papillomavirus-immortalized epithelial cells.
Keratinocytes can be induced to produce cytokines by exogenous stimuli, such as UVB, and dysregulation of this production has been described in various skin diseases, including cancer. In this study, we compared the effect of UVB on the secretion of several cytokines involved in inflammation by human keratinocytes immortalized or not with human papillomavirus (HPV)16 or HPV38 at the mRNA and protein levels. We show that expression of the HPV E6/E7 oncoproteins influences not only the basal cytokine secretion profile of keratinocytes, but also its modulation upon UVB irradiation. In particular, UVB upregulates interleukin (IL)-6, IL-8 and transforming growth factor (TGF)-beta in HPV-immortalized cells to a higher extent than in control keratinocytes. Moreover, expression of other pro-inflammatory molecules such as S100A8/9 and interferon (IFN)-kappa was downregulated in HPV-immortalized cells. These data support the functional similarity between HPV16 and 38, and suggest an active role of these viruses in modulation of the inflammatory process.
Dell'Oste
Valentina
V
Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
Azzimonti
Barbara
B
Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
Mondini
Michele
M
NoToPharm s.r.l., Bioindustry Park del Canavese, Via Ribes 5, 10010 Colleretto Giacosa (TO), Italy.|Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
De Andrea
Marco
M
Department of Public Health and Microbiology, Medical School of Torino, Via Santena 9, 10126 Torino, Italy.|Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
Borgogna
Cinzia
C
Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
Mesturini
Riccardo
R
Department of Medical Sciences, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
Accardi
Rosita
R
International Agency for Research on Cancer-World Health Organization, 150 Cours Albert Thomas, 69372 Lyon, France.
Tommasino
Massimo
M
International Agency for Research on Cancer-World Health Organization, 150 Cours Albert Thomas, 69372 Lyon, France.
Landolfo
Santo
S
NoToPharm s.r.l., Bioindustry Park del Canavese, Via Ribes 5, 10010 Colleretto Giacosa (TO), Italy.|Department of Public Health and Microbiology, Medical School of Torino, Via Santena 9, 10126 Torino, Italy.
Dianzani
Umberto
U
International Agency for Research on Cancer-World Health Organization, 150 Cours Albert Thomas, 69372 Lyon, France.
Gariglio
Marisa
M
NoToPharm s.r.l., Bioindustry Park del Canavese, Via Ribes 5, 10010 Colleretto Giacosa (TO), Italy.|Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
D052686:Betapapillomavirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D016207:Cytokines / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D005786:Gene Expression Regulation; D052162:Human papillomavirus 16 / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D007249:Inflammation; D015603:Keratinocytes / Q000276:immunology / Q000821:virology; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
D016207:Cytokines; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16