A reliable whole cell clamp technique.
This article describes a simple whole cell formation technique that the author invented in teaching and experiments. The implementation of the invented technique is a syringe with a hole and slot. With the newly invented technique, novices will shorten their learning curve and veterans will increase their success rate. The invented technique lightens the labor of the experimenter and improves the success rate and quality of whole cell preparations. The article also provides an idea to design an automated whole cell formation implementation. The tools developed in this technique make the patch-clamp experiment easy to teach and learn.
Li
Chenhong
C
Key Laboratory of Molecular Biophysics of Ministry Education of China, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China. lichhhust@yahoo.com
D004594:Electrophysiology / Q000193:education*; D018408:Patch-Clamp Techniques / Q000379:methods*