Effect of RNA interference of iASPP on the apoptosis in MCF-7 breast cancer cells.
ASPP family is proved to be apoptotic specific regulators of p53. Among them, iASPP acts as an inhibitor of p53. To investigate the effect of the iASPP RNAi on the apoptosis of breast cancer cell MCF-7, we transfected the recombinant plasmid PGCsilencer H1/Neo/GFP/RNAi into MCF-7. The iASPP expression was analyzed by RT-PCR and Western blot. The cell apoptosis was detected by FCM. The results show that the expression of iASPP is descended and the apoptosis rate is increased after transfection. Therefore, we conclude that inhibition of expression of iASPP may resume the ability of p53 to induce apoptosis in MCF-7 cells.
Liu
Ze-Jun
ZJ
Laboratory of International Cooperation, Southwest Hospital, Third Military Medical University, Chongqing, PRC. zejunliu2000@yahoo.com.cn
Cai
Yun
Y
Hou
Lu
L
Gao
Xing
X
Xin
Hai-Ming
HM
Lu
Xin
X
Zhong
Shan
S
Gu
Shou-Zhi
SZ
Chen
Jie
J
D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics; D034622:RNA Interference; D012097:Repressor Proteins; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; C119612:PPP1R13L protein, human; D012097:Repressor Proteins; D016159:Tumor Suppressor Protein p53