title: The yeast lysosome-like vacuole: endpoint and crossroads.
abstract: Fungal vacuoles are acidic organelles with degradative and storage capabilities that have many similarities to mammalian lysosomes and plant vacuoles. In the past several years, well-developed genetic, genomic, biochemical and cell biological tools in S. cerevisiae have provided fresh insights into vacuolar protein sorting, organelle acidification, ion homeostasis, autophagy, and stress-related functions of the vacuole, and these insights have often found parallels in mammalian lysosomes. This review provides a broad overview of the defining features and functions of S. cerevisiae vacuoles and compares these features to mammalian lysosomes. Recent research challenges the traditional view of vacuoles and lysosomes as simply the terminal compartment of biosynthetic and endocytic pathways (i.e. the "garbage dump" of the cell), and suggests instead that these compartments are unexpectedly dynamic and highly regulated.
authors.affiliation: Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA.
authors.forename: Sheena Claire
authors.initials: SC
authors.lastname: Li
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Kane
mesh_terms: D000143:Acids / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013312:Stress, Physiological; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D000143:Acids
references.citation: Eukaryot Cell. 2007 Jul;6(7):1166-77
references.pmid: 17526722
references.citation: Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3254-9
references.pmid: 10716729
references.citation: Mol Biol Cell. 2004 Aug;15(8):3553-66
references.pmid: 15155809
references.citation: J Biol Chem. 2001 Jun 29;276(26):23849-57
references.pmid: 11274162
references.citation: FEBS Lett. 2006 Feb 6;580(3):717-9
references.pmid: 16413548
references.citation: Am J Physiol Renal Physiol. 2007 Dec;293(6):F1915-26
references.pmid: 17898041
references.citation: Biochemistry. 1991 Nov 26;30(47):11212-20
references.pmid: 1835654
references.citation: J Biochem. 1997 Feb;121(2):338-44
references.pmid: 9089409
references.citation: J Biol Chem. 2003 Jun 13;278(24):22023-30
references.pmid: 12672822
references.citation: Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9565-70
references.pmid: 15972809
references.citation: Mol Cell Proteomics. 2007 Mar;6(3):394-412
references.pmid: 17151019
references.citation: Annu Rev Genet. 2003;37:435-60
references.pmid: 14616069
references.citation: EMBO J. 2000 Jun 15;19(12):2845-55
references.pmid: 10856230
references.citation: Microbiol Rev. 1990 Sep;54(3):266-92
references.pmid: 2215422
references.citation: J Biol Chem. 1990 Oct 15;265(29):17428-31
references.pmid: 2145275
references.citation: J Biol Chem. 2001 Aug 3;276(31):29515-9
references.pmid: 11390404
references.citation: Mol Cell Biol. 1998 Dec;18(12):7064-74
references.pmid: 9819393
references.citation: J Biol Chem. 2003 May 30;278(22):20013-8
references.pmid: 12649290
references.citation: Nature. 2003 Oct 16;425(6959):686-91
references.pmid: 14562095
references.citation: Annu Rev Genet. 2000;34:255-295
references.pmid: 11092829
references.citation: Curr Opin Nephrol Hypertens. 2002 Sep;11(5):563-8
references.pmid: 12187322
references.citation: J Biol Chem. 2004 Feb 6;279(6):4498-506
references.pmid: 14610088
references.citation: J Cell Biol. 1997 Dec 29;139(7):1761-74
references.pmid: 9412470
references.citation: Mol Cell Biol. 1990 Jul;10(7):3737-49
references.pmid: 2141385
references.citation: Biochem Biophys Res Commun. 1995 Jul 6;212(1):255-62
references.pmid: 7612014
references.citation: Nat Rev Mol Cell Biol. 2007 Nov;8(11):917-29
references.pmid: 17912264
references.citation: J Biol Chem. 1996 Sep 20;271(38):23061-7
references.pmid: 8798496
references.citation: J Biol Chem. 1991 Sep 15;266(26):17707-12
references.pmid: 1832676
references.citation: Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5001-6
references.pmid: 10220408
references.citation: FEBS Lett. 2002 Jun 5;520(1-3):63-7
references.pmid: 12044871
references.citation: J Biol Chem. 2002 Jan 4;277(1):763-73
references.pmid: 11675395
references.citation: FEBS J. 2005 May;272(10):2497-511
references.pmid: 15885099
references.citation: Microbiol Mol Biol Rev. 2002 Jun;66(2):300-72
references.pmid: 12040128
references.citation: Biochemistry. 1989 Apr 4;28(7):3075-82
references.pmid: 2568125
references.citation: Eukaryot Cell. 2006 Apr;5(4):723-31
references.pmid: 16607019
references.citation: FEBS Lett. 2000 Mar 10;469(2-3):137-41
references.pmid: 10713258
references.citation: Nat Cell Biol. 2005 Feb;7(2):103-4
references.pmid: 15689974
references.citation: J Biol Chem. 2001 May 25;276(21):17941-8
references.pmid: 11278748
references.citation: J Biol Chem. 2000 Mar 24;275(12):8760-5
references.pmid: 10722719
references.citation: Science. 2003 Feb 28;299(5611):1400-3
references.pmid: 12610307
references.citation: Science. 1989 Aug 25;245(4920):855-7
references.pmid: 2528207
references.citation: Mol Biol Cell. 1992 Dec;3(12):1389-402
references.pmid: 1493335
references.citation: EMBO J. 2004 May 5;23(9):1922-33
references.pmid: 15103325
references.citation: J Cell Biol. 2002 Aug 5;158(3):389-94
references.pmid: 12147679
references.citation: EMBO J. 2001 Aug 1;20(15):4035-40
references.pmid: 11483507
references.citation: Hum Mol Genet. 2000 Aug 12;9(13):2059-63
references.pmid: 10942435
references.citation: Mol Biol Cell. 2005 Mar;16(3):1396-405
references.pmid: 15635088
references.citation: Traffic. 2006 Aug;7(8):1045-59
references.pmid: 16734661
references.citation: EMBO J. 2002 Mar 15;21(6):1241-7
references.pmid: 11889030
references.citation: Eukaryot Cell. 2002 Jun;1(3):391-400
references.pmid: 12455987
references.citation: J Biol Chem. 1999 Dec 24;274(52):37270-9
references.pmid: 10601292
references.citation: Proc Natl Acad Sci U S A. 1983 Jan;80(2):510-4
references.pmid: 6340101
references.citation: Cell. 2004 Nov 24;119(5):667-78
references.pmid: 15550248
references.citation: Mol Biol Cell. 1995 May;6(5):525-39
references.pmid: 7663021
references.citation: J Biol Chem. 2006 Apr 14;281(15):10273-80
references.pmid: 16423829
references.citation: Biochim Biophys Acta. 2005 Jul 10;1744(3):438-54
references.pmid: 15913810
references.citation: J Biol Chem. 2002 Apr 19;277(16):13831-9
references.pmid: 11844802
references.citation: Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12304-8
references.pmid: 8901576
references.citation: Annu Rev Genet. 2008;42:27-81
references.pmid: 18303986
references.citation: EMBO J. 1997 May 15;16(10):2769-82
references.pmid: 9184222
references.citation: Mol Cell Biol. 1996 May;16(5):2226-37
references.pmid: 8628289
references.citation: Proc Natl Acad Sci U S A. 1978 Jul;75(7):3327-31
references.pmid: 28524
references.citation: Biochem J. 2006 Apr 1;395(1):73-80
references.pmid: 16316315
references.citation: Mol Biol Cell. 2008 Jul;19(7):2962-72
references.pmid: 18434603
references.citation: J Cell Biol. 1981 Sep;90(3):656-64
references.pmid: 7287819
references.citation: Nature. 1998 Dec 10;396(6711):575-80
references.pmid: 9859992
references.citation: Cell Death Differ. 2005 Nov;12 Suppl 2:1542-52
references.pmid: 16247502
references.citation: J Biol Chem. 2001 Oct 26;276(43):40050-4
references.pmid: 11498539
references.citation: Cell. 1994 May 20;77(4):579-86
references.pmid: 8187177
references.citation: J Biol Chem. 2004 May 7;279(19):19698-704
references.pmid: 14993228
references.citation: Oncogene. 2006 Oct 16;25(48):6392-415
references.pmid: 17041625
references.citation: Nat Genet. 2000 May;25(1):25-9
references.pmid: 10802651
references.citation: Yeast. 1996 Jan;12(1):1-16
references.pmid: 8789256
references.citation: Genes Dev. 2007 Nov 15;21(22):2861-73
references.pmid: 18006683
references.citation: J Biol Chem. 1989 Sep 25;264(27):16037-45
references.pmid: 2674123
references.citation: Bioessays. 1999 Dec;21(12):1004-10
references.pmid: 10580985
references.citation: FEBS Lett. 1999 May 21;451(2):132-6
references.pmid: 10371152
references.citation: J Am Soc Nephrol. 2005 Nov;16(11):3235-46
references.pmid: 16177003
references.citation: J Biol Chem. 2004 Aug 6;279(32):33653-61
references.pmid: 15161905
references.citation: J Bone Miner Res. 1997 Jul;12(7):1116-23
references.pmid: 9200012
references.citation: Mol Biol Cell. 1995 Sep;6(9):1089-102
references.pmid: 8534908
references.citation: J Biol Chem. 2002 Oct 18;277(42):39187-94
references.pmid: 12161436
references.citation: Annu Rev Microbiol. 2002;56:237-61
references.pmid: 12142483
references.citation: J Biol Chem. 1996 Jul 26;271(30):17621-4
references.pmid: 8663607
references.citation: Proc Natl Acad Sci U S A. 1990 May;87(9):3503-7
references.pmid: 2139726
references.citation: Mol Biol Cell. 2004 Jan;15(1):24-36
references.pmid: 14528018
references.citation: Nat Rev Mol Cell Biol. 2006 Apr;7(4):243-52
references.pmid: 16607287
references.citation: Nature. 2008 Feb 28;451(7182):1069-75
references.pmid: 18305538
references.citation: Trends Biochem Sci. 2000 May;25(5):229-35
references.pmid: 10782093
references.citation: Mol Biol Cell. 2006 Oct;17(10):4411-9
references.pmid: 16885415
references.citation: J Biol Chem. 2004 Feb 6;279(6):4322-9
references.pmid: 14594803
references.citation: Mol Biol Cell. 2008 Jun;19(6):2500-8
references.pmid: 18385512
references.citation: J Biol Chem. 1993 May 15;268(14):10564-72
references.pmid: 8486710
references.citation: Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13641-5
references.pmid: 9811853
references.citation: Biol Reprod. 2006 Jan;74(1):185-94
references.pmid: 16192400
references.citation: J Biol Chem. 1992 Feb 15;267(5):3416-22
references.pmid: 1531340
references.citation: Eukaryot Cell. 2007 Nov;6(11):2009-17
references.pmid: 17905927
references.citation: J Bacteriol. 1991 Mar;173(6):2026-34
references.pmid: 2002005
references.citation: Nat Rev Mol Cell Biol. 2002 Dec;3(12):893-905
references.pmid: 12461556
references.citation: Microbiol Mol Biol Rev. 2006 Mar;70(1):177-91
references.pmid: 16524922
references.citation: Biochem Biophys Res Commun. 2003 Nov 28;311(4):1143-50
references.pmid: 14623300
references.citation: J Biol Chem. 2008 Jul 18;283(29):20309-19
references.pmid: 18502746
references.citation: Proc Natl Acad Sci U S A. 2007 May 22;104(21):8755-60
references.pmid: 17502611
references.citation: J Biol Chem. 2003 Apr 11;278(15):12985-91
references.pmid: 12569096
references.citation: Microbiology (Reading). 1999 Nov;145 ( Pt 11):3221-3228
references.pmid: 10589731
references.citation: Plant Physiol. 2007 Sep;145(1):216-29
references.pmid: 17660356
references.citation: Hum Mol Genet. 2007 May 1;16(9):1007-16
references.pmid: 17341489
references.citation: J Biol Chem. 1992 Sep 15;267(26):18665-70
references.pmid: 1526998
references.citation: J Cell Biol. 1997 Aug 11;138(3):531-45
references.pmid: 9245784
references.citation: J Cell Biol. 1990 Sep;111(3):877-92
references.pmid: 2202738
references.citation: J Cell Biol. 1998 Aug 10;142(3):651-63
references.pmid: 9700156
references.citation: J Biol Chem. 2005 Feb 11;280(6):4851-7
references.pmid: 15572352
references.citation: Mol Biol Cell. 2000 Dec;11(12):4309-21
references.pmid: 11102525
references.citation: J Biol Chem. 2004 Mar 5;279(10):8732-9
references.pmid: 14672945
references.citation: J Biol Chem. 2005 Sep 9;280(36):31582-6
references.pmid: 16027116
references.citation: J Biol Chem. 2001 Aug 10;276(32):30407-13
references.pmid: 11399750
references.citation: J Biol Chem. 2001 Dec 14;276(50):47411-20
references.pmid: 11592965
references.citation: Mol Biol Cell. 2007 Nov;18(11):4232-44
references.pmid: 17699591
references.citation: J Cell Biol. 2003 Jul 21;162(2):211-22
references.pmid: 12876274
references.citation: J Cell Biol. 1981 Sep;90(3):665-9
references.pmid: 6169733
references.citation: Cell. 1996 Feb 9;84(3):395-400
references.pmid: 8608593
references.citation: Curr Biol. 2002 Jun 4;12(11):885-93
references.pmid: 12062051
references.citation: J Cell Biol. 2002 Aug 19;158(4):761-72
references.pmid: 12186856
references.citation: J Cell Biol. 2004 Dec 20;167(6):1087-98
references.pmid: 15611334
references.citation: J Biol Chem. 2007 Nov 16;282(46):33735-33742
references.pmid: 17872947
references.citation: J Biol Chem. 2007 Jun 8;282(23):16736-43
references.pmid: 17428789
references.citation: EMBO J. 1989 May;8(5):1349-59
references.pmid: 2670552
references.citation: J Cell Biol. 2003 Dec 8;163(5):973-85
references.pmid: 14662743
references.citation: Immunity. 1998 May;8(5):647-56
references.pmid: 9620685
references.citation: Proc Natl Acad Sci U S A. 1988 Nov;85(21):7972-6
references.pmid: 2973058
references.citation: J Cell Biol. 2002 Jan 7;156(1):29-34
references.pmid: 11781332
references.citation: J Cell Biol. 1992 Nov;119(4):773-86
references.pmid: 1429836
references.citation: J Biol Chem. 2002 Aug 23;277(34):31079-88
references.pmid: 12058033
references.citation: FEBS J. 2007 Aug;274(16):4287-305
references.pmid: 17651441
references.citation: J Cell Biol. 2002 Mar 18;156(6):1015-28
references.pmid: 11889142
references.citation: Mol Cell. 2005 Jul 1;19(1):15-26
references.pmid: 15989961
references.citation: J Cell Biol. 2006 Feb 27;172(5):693-704
references.pmid: 16492811
references.citation: Proc Natl Acad Sci U S A. 2003 May 13;100(10):5813-8
references.pmid: 12724530
references.citation: Nat Biotechnol. 2004 Jan;22(1):62-9
references.pmid: 14661025
references.citation: Dev Biol. 2000 Dec 15;228(2):315-25
references.pmid: 11112332
references.citation: Annu Rev Biochem. 2000;69:303-42
references.pmid: 10966461
references.citation: Autophagy. 2007 Sep-Oct;3(5):417-21
references.pmid: 17534141
references.citation: J Cell Biol. 1992 Oct;119(2):301-11
references.pmid: 1400575
references.citation: Mol Biol Cell. 2006 Dec;17(12):5094-104
references.pmid: 17021250
references.citation: Annu Rev Biophys Biomol Struct. 2006;35:277-98
references.pmid: 16689637
references.citation: Curr Opin Cell Biol. 1993 Dec;5(6):990-6
references.pmid: 8129953
references.citation: J Cell Biol. 1988 Oct;107(4):1369-83
references.pmid: 3049619
references.citation: J Cell Sci. 2006 Nov 1;119(Pt 21):4531-40
references.pmid: 17046993
references.citation: J Cell Biol. 1993 Jun;121(6):1245-56
references.pmid: 8509446
references.citation: J Cell Biol. 1995 Nov;131(3):603-17
references.pmid: 7593183
references.citation: EMBO J. 1989 Jul;8(7):2057-65
references.pmid: 2676511
references.citation: J Biol Chem. 1994 Mar 25;269(12):8792-6
references.pmid: 8132612
references.citation: Genetics. 1996 Oct;144(2):445-58
references.pmid: 8889511
references.citation: Mol Gen Genet. 1993 Nov;241(3-4):447-56
references.pmid: 8246899
references.citation: Nature. 1997 Nov 13;390(6656):187-92
references.pmid: 9367158
references.citation: Traffic. 2005 Jan;6(1):2-9
references.pmid: 15569240
references.citation: Trends Biochem Sci. 2002 Feb;27(2):87-93
references.pmid: 11852246
references.citation: Curr Opin Cell Biol. 2005 Aug;17(4):402-8
references.pmid: 15975782
references.citation: J Biol Chem. 1990 Dec 15;265(35):21940-5
references.pmid: 2147689
references.citation: J Biol Chem. 2005 Apr 29;280(17):16754-62
references.pmid: 15737991
references.citation: J Biol Chem. 2001 Jan 19;276(3):2083-7
references.pmid: 11085977
references.citation: J Cell Biol. 2004 Jan 19;164(2):195-206
references.pmid: 14734531
references.citation: Cell. 2002 Feb 8;108(3):357-69
references.pmid: 11853670
references.citation: EMBO J. 1991 Jul;10(7):1741-8
references.pmid: 2050111
references.citation: J Biol Chem. 1996 May 3;271(18):10946-52
references.pmid: 8631913
references.citation: J Bioenerg Biomembr. 2005 Dec;37(6):425-9
references.pmid: 16691477
references.citation: Nature. 2001 Feb 1;409(6820):581-8
references.pmid: 11214310
references.citation: J Biol Chem. 2007 Sep 28;282(39):28619-28626
references.pmid: 17681937
references.citation: Mol Cell Biol. 1997 Dec;17(12):6847-58
references.pmid: 9372916
references.citation: Eukaryot Cell. 2004 Feb;3(1):1-13
references.pmid: 14871932
references.citation: J Cell Sci. 2003 Apr 15;116(Pt 8):1637-46
references.pmid: 12640047
references.citation: J Biol Chem. 2000 Dec 8;275(49):38245-53
references.pmid: 10991947
references.citation: Cell. 1998 Mar 20;92(6):759-72
references.pmid: 9529252
references.citation: J Biol Chem. 2007 Aug 24;282(34):24495-503
references.pmid: 17576770
references.citation: Cell. 1996 Jan 26;84(2):277-87
references.pmid: 8565073
references.citation: Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7105-10
references.pmid: 12771382
references.citation: J Cell Biol. 1998 Oct 5;143(1):65-79
references.pmid: 9763421
references.citation: J Biol Chem. 1998 Dec 11;273(50):33449-54
references.pmid: 9837923
references.citation: Biochim Biophys Acta. 2007 Mar;1771(3):353-404
references.pmid: 17382260
references.citation: J Cell Biol. 2001 May 14;153(4):649-62
references.pmid: 11352928
references.citation: Mol Biol Cell. 2003 Jan;14(1):129-41
references.pmid: 12529432
references.citation: Nat Rev Mol Cell Biol. 2007 Aug;8(8):622-32
references.pmid: 17637737
references.citation: Mol Biol Cell. 2007 Apr;18(4):1480-9
references.pmid: 17314395
references.citation: J Biol Chem. 2007 Aug 24;282(34):24743-51
references.pmid: 17565997
references.citation: Annu Rev Nutr. 2007;27:19-40
references.pmid: 17311494
references.citation: J Cell Biol. 1993 Jul;122(1):53-65
references.pmid: 8391002
references.citation: J Biol Chem. 1996 Nov 29;271(48):30677-84
references.pmid: 8940044
references.citation: Genome Biol. 2006;7(11):R109
references.pmid: 17107617
references.citation: J Biol Chem. 1994 May 13;269(19):14064-74
references.pmid: 7514599
references.citation: Biochem Biophys Res Commun. 1993 Mar 31;191(3):1111-7
references.pmid: 8466488
references.citation: Proc Natl Acad Sci U S A. 1990 Feb;87(3):1076-80
references.pmid: 1689059
references.citation: Cell. 1991 Nov 1;67(3):495-504
references.pmid: 1934057
references.citation: Annu Rev Biochem. 1986;55:663-700
references.pmid: 2874766
references.citation: Microsc Res Tech. 2000 Dec 15;51(6):563-72
references.pmid: 11169858
references.citation: J Bacteriol. 1999 Mar;181(6):1793-800
references.pmid: 10074071
references.citation: J Bacteriol. 2001 Oct;183(20):5942-55
references.pmid: 11566994
references.citation: Mol Biol Cell. 2007 Oct;18(10):3873-82
references.pmid: 17652457
references.citation: Autophagy. 2008 Jan;4(1):5-19
references.pmid: 17932463
references.citation: J Biol Chem. 1991 May 5;266(13):7963-6
references.pmid: 2022624
references.citation: Physiology (Bethesda). 2004 Aug;19:207-15
references.pmid: 15304635
references.citation: Am J Physiol Cell Physiol. 2007 Jul;293(1):C199-210
references.pmid: 17392376