Measurement of intracellular calcium ions by flow cytometry.
Using flow cytometry, single-cell measurements of calcium can be made on isolated populations identified by one or more phenotypic characteristics. This unit describes the preparation of cells, including labeling with antibodies and with calcium probes, and discusses the principles of data analysis and interpretation.
June
C H
CH
Naval Medical Research Institute, Bethesda, Maryland, USA.
Abe
R
R
Rabinovitch
P S
PS
D000818:Animals; D002118:Calcium / Q000032:analysis*; D002469:Cell Separation / Q000379:methods*; D003593:Cytoplasm / Q000378:metabolism; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007477:Ions; D010641:Phenotype
D005456:Fluorescent Dyes; D007477:Ions; D002118:Calcium