title: Immunohistochemical evidence of ubiquitous distribution of the metalloendoprotease insulin-degrading enzyme (IDE; insulysin) in human non-malignant tissues and tumor cell lines.
abstract: Immunohistochemical evidence of ubiquitous distribution of the metalloprotease insulin-degrading enzyme (IDE; insulysin) in human non-malignant tissues and tumor cells is presented. Immunohistochemical staining was performed on a multi-organ tissue microarray (pancreas, lung, kidney, central/peripheral nervous system, liver, breast, placenta, myocardium, striated muscle, bone marrow, thymus, and spleen) and on a cell microarray of 31 tumor cell lines of different origin, as well as trophoblast cells and normal blood lymphocytes and granulocytes. IDE protein was expressed in all the tissues assessed and all the tumor cell lines except for Raji and HL-60. Trophoblast cells and granulocytes, but not normal lymphocytes, were also IDE-positive.
authors.affiliation: Institute of Pathology, Klinikum rechts der Isar, Technical University of Munich, Ismaninger Str. 22, D-81675 Munich, Germany.
authors.forename: Gregor
authors.initials: G
authors.lastname: Weirich
authors.forename: Karin
authors.initials: K
authors.lastname: Mengele
authors.forename: Christina
authors.initials: C
authors.lastname: Yfanti
authors.forename: Apostolos
authors.initials: A
authors.lastname: Gkazepis
authors.forename: Daniela
authors.initials: D
authors.lastname: Hellmann
authors.forename: Anita
authors.initials: A
authors.lastname: Welk
authors.forename: Cecylia
authors.initials: C
authors.lastname: Giersig
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Kuo
authors.forename: Marsha Rich
authors.initials: MR
authors.lastname: Rosner
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Tang
authors.forename: Manfred
authors.initials: M
authors.lastname: Schmitt
mesh_terms: D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007339:Insulysin / Q000032:analysis / Q000097:blood / Q000276:immunology* / Q000378:metabolism*; D009928:Organ Specificity
chemical_list: D007339:Insulysin
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