Idaten is a new cold-inducible transposon of Volvox carteri that can be used for tagging developmentally important genes.
A cold-inducible transposon called Jordan has previously been used to tag and recover genes controlling key aspects of Volvox development, including the process called inversion. In a search for additional genes, we isolated 17 new inversionless mutants from cultures grown at 24 degrees (the temperature that activates Jordan transposition). These mutants were stable at 32 degrees, but generated revertants at 24 degrees . DNA blots revealed that one mutant had a transposon unrelated to Jordan inserted in invA ("inversionless A"). This new transposon, which we named Idaten, has terminal inverted repeats (TIRs) beginning with CCCTA, and upon insertion it creates a 3-bp target-site duplication. It appears to belong to the CACTA superfamily of class II DNA transposons, which includes En/Spm. No significant open reading frames were in the Idaten sequence, but we retrieved another element with Idaten-type TIRs encoding a protein similar to the En/Spm transposase as a candidate for an Idaten-specific transposase. We found that in five of the new inversionless strains we could not find any Jordan insertions causing the phenotype to possess insertions of an Idaten family member in a single locus (invC). This clearly indicates that Idaten is a potentially powerful alternative to Jordan for tagging developmentally important genes in Volvox.
Ueki
Noriko
N
RIKEN Advanced Science Institute, Wako-shi, Saitama, 351-0198, Japan.
Nishii
Ichiro
I
D020418:Algal Proteins / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003080:Cold Temperature; D004251:DNA Transposable Elements / Q000235:genetics*; D048028:DNA, Algal / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018745:Genome, Plant; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction; D018626:Retroelements / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D020079:Terminal Repeat Sequences / Q000235:genetics; D019895:Transposases; D044446:Volvox / Q000502:physiology*
D020418:Algal Proteins; D004251:DNA Transposable Elements; D048028:DNA, Algal; D018744:DNA, Plant; D018626:Retroelements; D019895:Transposases
Anal Biochem. 1986 Feb 1;152(2):232-8
3516005
PLoS Genet. 2006 Nov 10;2(11):e169
17096595
Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7469-74
9207115
Science. 2007 Oct 12;318(5848):245-50
17932292
Bioessays. 2005 Mar;27(3):299-310
15714559
Protist. 2006 Oct;157(4):445-61
16854623
Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10370-4
8234300
Brief Funct Genomic Proteomic. 2003 Apr;2(1):57-71
15239944
Symp Soc Dev Biol. 1970;29:59-100
4950154
Plant Cell. 1993 Sep;5(9):1125-38
8400878
Cell. 2003 Jun 13;113(6):743-53
12809605
Glycobiology. 2005 Oct;15(10):912-23
15958417
Curr Opin Microbiol. 2003 Dec;6(6):608-13
14662357
Plant Cell. 2006 Jan;18(1):104-18
16326924
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
15034147
Genesis. 2006 Sep;44(9):438-45
16906529
Dev Dyn. 2005 Oct;234(2):244-54
16110506
J Mol Evol. 1989 Sep;29(3):255-65
2506359
Dev Cell. 2004 Jul;7(1):133-44
15239961
Development. 1999 Feb;126(4):649-58
9895313
EMBO J. 1986 May;5(5):835-41
15957213
Mol Gen Genet. 1991 Aug;228(1-2):201-8
1715971
Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12315-6
11675478
BMC Evol Biol. 2006 Nov 07;6:89
17090310
Development. 1999 Feb;126(4):639-47
9895312
Dev Growth Differ. 2001 Dec;43(6):621-31
11737143
Proc Natl Acad Sci U S A. 2007 May 15;104 Suppl 1:8613-8
17494748
Plant Mol Biol. 1992 May;19(1):39-49
1318114
Dev Biol. 1983 Apr;96(2):493-506
6832480
Nat Rev Genet. 2007 Dec;8(12):973-82
17984973