Complete primary structure of a newly characterized galactose-specific lectin from the seeds of Dolichos lablab.
A new unique lectin (galactose-specific) purified from the seeds of Dolichos lablab, designated as DLL-II is a heterodimer composed of closely related subunits alpha and beta. These were separated by SDS-PAGE and isolated by electroelution. By ESI-MS analysis their molecular masses were found to be 30.746 kDa (alpha) and 28.815 kDa (beta) respectively. Both subunits were glycosylated and displayed similar amino acid composition. Using advanced mass spectrometry in combination with de novo sequencing and database searches for the peptides derived by enzymatic and chemical cleavage of these subunits, the primary sequence was deduced. This revealed DLL-II to be made of two polypeptide chains of 281(alpha) and 263(beta) amino acids respectively. The beta subunit differed from the alpha subunit by the absence of some amino acids at the carboxy terminal end. This structural difference suggests that possibly, the beta subunit is derived from the alpha subunit by posttranslational proteolytic modification at the COOH-terminus. Comparison of the DLL-II sequence to other leguminous seed lectins indicates a high degree of structural conservation.
Rameshwaram
Nagender Rao
NR
Department of Biochemistry, University of Hyderabad, India.
Karanam
Narasimha Kumar
NK
Scharf
Christian
C
Völker
Uwe
U
Nadimpalli
Siva Kumar
SK
D000595:Amino Acid Sequence; D031294:Dolichos / Q000378:metabolism*; D005690:Galactose / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000737:chemistry*; D016415:Sequence Alignment
D037121:Plant Lectins; D005690:Galactose
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