Analysis of acamprosate in beagle dog plasma by LC-MS-MS.
A rapid, sensitive, and specific analytical method was developed and validated to quantify acamprosate calcium in beagle dog plasma. The method employs a single plasma protein precipitation, and the analytes are separated by chromatography on an Acquity UPLC HSS T3 column and analyzed by mass spectrometry in the multiple reaction monitoring (MRM) mode. The method has a chromatographic run time of 1.25 min and a linear calibration curve over the range 200-10000 ng/mL (r(2)>0.9994). The intra-day and inter-day accuracy and precision were within 10.0% for the analyte. Acamprosate was stable during all sample storage, preparation, and analytical periods. This method was employed in a pharmacokinetic study of an acamprosate 333 mg enteric-coated tablet in 8 male beagle dogs that received single 666 mg doses (333 mg x 2 tablets). The proposed method enables identification and quantification in pharmacokinetic studies of acamprosate in beagle dog plasma.
Rhee
Yun-Seok
YS
School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea.
Park
Jeong-Hwa
JH
Park
Seok
S
Park
Chun-Woong
CW
Ha
Jeong-Myung
JM
Jeong
Ki-Woo
KW
Lee
Dong-Soo
DS
Park
Eun-Seok
ES
D000077443:Acamprosate; D000427:Alcohol Deterrents / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004285:Dogs; D005615:Freezing; D008297:Male; D013058:Mass Spectrometry; D011786:Quality Control; D012015:Reference Standards; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization; D013608:Tablets, Enteric-Coated; D013654:Taurine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
D000427:Alcohol Deterrents; D012996:Solutions; D013608:Tablets, Enteric-Coated; D013654:Taurine; D000077443:Acamprosate