Fluorescence nanoscopy by ground-state depletion and single-molecule return.
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.
Fölling
Jonas
J
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
Bossi
Mariano
M
Bock
Hannes
H
Medda
Rebecca
R
Wurm
Christian A
CA
Hein
Birka
B
Jakobs
Stefan
S
Eggeling
Christian
C
Hell
Stefan W
SW
D002460:Cell Line; D002470:Cell Survival; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*