title: Polyamine biosynthesis regulated by StARD expression plays an important role in potato wound periderm formation.
abstract: An acireductone dioxygenase (ARD) gene of potatoes was isolated from the expressed sequence tags (ESTs) of potato post-suberization cDNA libraries. The highest expression levels of the StARD gene and the protein appeared 36 h after suberization. An approximate 9-fold increase in ARD activity was detected at 36 h after wounding. Real-time reverse transcription-PCR (RT-PCR) analysis and immunolocalization studies revealed that StARD transcripts increase at the wound surface of potato tubers. The polyamine (PA) contents increased significantly after wounding at the wound surface. The increased PA content and ARD activity may play an important role in wound periderm formation.
authors.affiliation: Plant Genome Research Center, KRIBB, Daejeon 305-806, Korea.
authors.forename: Jae Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Hyun Soon
authors.initials: HS
authors.lastname: Kim
authors.forename: Yong Hwa
authors.initials: YH
authors.lastname: Lee
authors.forename: Yoon Sik
authors.initials: YS
authors.lastname: Kim
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Oh
authors.forename: Hyouk
authors.initials: H
authors.lastname: Joung
authors.forename: Suhn Kee
authors.initials: SK
authors.lastname: Chae
authors.forename: Kyong Hoon
authors.initials: KH
authors.lastname: Suh
authors.forename: Jae Heung
authors.initials: JH
authors.lastname: Jeon
mesh_terms: D049308:Dioxygenases / Q000378:metabolism*; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D008055:Lipids / Q000096:biosynthesis; D008715:Methionine / Q000378:metabolism; D035281:Plant Tubers / Q000235:genetics / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics
chemical_list: D008055:Lipids; D011073:Polyamines; D018749:RNA, Plant; C065875:suberin; D008715:Methionine; D049308:Dioxygenases; C083734:aci-reductone oxidase (CO-forming)