Helicobacter pylori induces gastritis and oxidative stress after distal gastrectomy.
OBJECTIVE
Helicobacter pylori is a carcinogen for gastric cancer. There have been few reports on carcinogenesis about H. pylori infection in the gastric remnant after distal gastrectomy. The relationship between carcinogenesis and H. pylori infection was studied by means of inflammation and oxidative stress.


METHODS
Ninety-seven patients who had undergone curative distal gastrectomy for gastric cancer were studied. All patients underwent endoscopic examination 3 months after surgery. The presence of H. pylori was determined using urease rapid test, hematoxylin-eosin staining, and immunohistochemical staining. Fifty-one patients were positive (group A) and 46 patients were negative (group B). The grades of remnant gastritis were scored by updated Sydney System. 8-Hydroxydeoxyguanosine (8-OHdG) as an oxidative stress marker, was analyzed immunohistochemically, and graded in 4 grades.


RESULTS
Both the neutrophil infiltration score and 8-OHdG expression score was higher in group A than group B (p = 0.03 and 0.05). The correlation between the 8-OHdG expression score and the neutrophil infiltration score was significant (p = 0.02).


CONCLUSIONS
As gastritis is related to oxidative stress, H. pylori infection is suspected to play an important role in carcinogenesis in the gastric remnant.
Fukuhara
Kenichiro
K
Department of Gastroenterological Surgery, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abenoku, Osaka 545-8585, Japan. fuku@eva.hi-ho.ne.jp
Osugi
Harushi
H
Takemura
Masashi
M
Lee
Shigeru
S
Morimura
Keiichiro
K
Kishida
Satoru
S
Nishizawa
Satoshi
S
Iwasaki
Hiroshi
H
Gyobu
Ken
K
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005500:Follow-Up Studies; D005743:Gastrectomy / Q000009:adverse effects*; D005753:Gastric Mucosa / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D018530:Gastric Stump; D005756:Gastritis / Q000175:diagnosis / Q000378:metabolism / Q000382:microbiology*; D005763:Gastroenterostomy; D016481:Helicobacter Infections / Q000175:diagnosis / Q000209:etiology* / Q000378:metabolism; D016480:Helicobacter pylori; D006801:Humans; D018384:Oxidative Stress; D013274:Stomach Neoplasms / Q000378:metabolism / Q000382:microbiology* / Q000601:surgery
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine