title: Central nervous system extracellular matrix changes in a transgenic mouse model of bovine spongiform encephalopathy.
abstract: Bovine spongiform encephalopathy (BSE) is a transmissible spongiform encephalopathy characterised by accumulation of resistant prion protein (PrP(BSE)), neuronal loss, spongiosus and glial cell proliferation. In this study, properties of the extracellular matrix (ECM) were investigated in boTg110 transgenic mice over-expressing the bovine cellular prion protein (PrP(c)) and infected with BSE. Using immunohistochemistry with Wisteria floribunda agglutinin as a specific marker for perineuronal nets (PNNs) and antibodies against aggrecan and hyaluronic acid binding protein, loss of ECM was correlated with PrP(BSE) accumulation and activation of astrocytes and microglia. PrP(BSE) accumulation and glial cell activation were detected from the earliest stages of the disease and increased in the terminal stages. Decreases in PNNs, aggrecan and hyaluronic acid were observed only in the terminal stages and correlated with the distribution of PrP(BSE) and activated glial cells. This study suggests that the loss of PNNs, aggrecan and hyaluronic acid is a consequence of PrP(BSE) accumulation. Degradation of ECM in BSE may be due to secretion of degradative enzymes by activated glial cells.
authors.affiliation: Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain.
authors.forename: Carme
authors.initials: C
authors.lastname: Costa
authors.forename: Raül
authors.initials: R
authors.lastname: Tortosa
authors.forename: Enric
authors.initials: E
authors.lastname: Vidal
authors.forename: Danielle
authors.initials: D
authors.lastname: Padilla
authors.forename: Juan Maria
authors.initials: JM
authors.lastname: Torres
authors.forename: Isidre
authors.initials: I
authors.lastname: Ferrer
authors.forename: Martí
authors.initials: M
authors.lastname: Pumarola
authors.forename: Anna
authors.initials: A
authors.lastname: Bassols
mesh_terms: D000818:Animals; D002417:Cattle; D002490:Central Nervous System / Q000378:metabolism* / Q000473:pathology; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism* / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism* / Q000473:pathology; D005904:Glial Fibrillary Acidic Protein; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology; D011328:Prions / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D011328:Prions; C528900:glial fibrillary astrocytic protein, mouse