Isolation of small RNAs using biotinylated PNAs.
In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from human total RNA in pure form. Damage to the RNA appears to be negligible by this method because the method is rapid and does not require a high temperature treatment to facilitate RNA-PNA binding.
Ohtsuki
Takashi
T
Department of Bioscience and Biotechnology, Okayama University, Tsushimanaka, Okayama, Japan. ohtsuk@cc.okayama-u.ac.jp
Fujimoto
Takeshi
T
Kamimukai
Maya
M
Kumano
Chisato
C
Kitamatsu
Mizuki
M
Sisido
Masahiko
M
D000818:Animals; D001483:Base Sequence; D019921:Biotinylation; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008722:Methods; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020135:Peptide Nucleic Acids / Q000737:chemistry; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020135:Peptide Nucleic Acids; D012329:RNA, Bacterial; D012356:RNA, Transfer, Leu; D012313:RNA