Zinc increases the phagocytic capacity of canine peripheral blood phagocytes in vitro.
Zinc is a trace element that plays a central role in the immune system. In the present study, the effect of zinc on the phagocytic capacity of canine peripheral blood phagocytes was examined in vitro by flow cytometry. Zinc was used at a concentration of 100 microM, which preserved cell viability. Treatment with zinc did not directly affect the phagocytic capacity of peripheral blood polymorphonuclear neutrophils (PMN) and mononuclear cells (PBMC). However, it did directly enhance the phagocytic capacity of peripheral blood monocyte-rich cells. Moreover, the phagocytic capacity of PMN and monocyte-rich cells but not PBMC was remarkably enhanced by culture supernatants from PBMC but not PMN treated with zinc. Anti-recombinant canine (rc) tumor necrosis factor-alpha (TNF-alpha) polyclonal antibody (pAb) neutralized the enhancing effect of the culture supernatant from zinc-treated PBMC and this supernatant had higher TNF-alpha levels than the culture supernatant of untreated PBMC. Thus, zinc may stimulate canine PBMC to produce TNF-alpha, which enhances the phagocytic capacity of canine peripheral blood phagocytes.
Kim
You-Joung
YJ
Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea.
Kang
Ji-Houn
JH
Yang
Mhan-Pyo
MP
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D009500:Neutralization Tests / Q000662:veterinary; D010587:Phagocytosis / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D019287:Zinc Sulfate / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha; D019287:Zinc Sulfate
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