title: Recent advances on the GAP promoter derived expression system of Pichia pastoris.
abstract: Pichia pastoris is an efficient host for the expression and secretion of heterologous proteins and the most important feature of P. pastoris is the existence of a strong and tightly regulated promoter from the alcohol oxidase I (AOX1) gene. The AOX1 promoter (pAOX1) has been used to express foreign genes and to produce a variety of recombinant proteins in P. pastoris. However, some efforts have been made to develop new alternative promoters to pAOX1 to avoid the use of methanol. The glyceraldehyde-3-phosphate dehydrogenase promoter (pGAP) has been used for constitutive expression of many heterologous proteins. The pGAP-based expression system is more suitable for large-scale production because the hazard and cost associated with the storage and delivery of large volume of methanol are eliminated. Some important developments and features of this expression system will be summarized in this review.
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China. zhangailian6@yahoo.com.cn
authors.forename: Ai-Lian
authors.initials: AL
authors.lastname: Zhang
authors.forename: Jin-Xian
authors.initials: JX
authors.lastname: Luo
authors.forename: Tian-Yuan
authors.initials: TY
authors.lastname: Zhang
authors.forename: Ying-Wen
authors.initials: YW
authors.lastname: Pan
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Tan
authors.forename: Ce-Yi
authors.initials: CY
authors.lastname: Fu
authors.forename: Fa-zhi
authors.initials: FZ
authors.lastname: Tu
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D010843:Pichia / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D005987:Glyceraldehyde-3-Phosphate Dehydrogenases
references.citation: Biotechnol Lett. 2005 Mar;27(5):339-45
references.pmid: 15834796
references.citation: Sheng Wu Gong Cheng Xue Bao. 2002 Sep;18(5):593-6
references.pmid: 12561205
references.citation: Biotechnol Lett. 2003 Jan;25(2):173-7
references.pmid: 12882295
references.citation: Biotechnol Bioeng. 2000 May 5;68(3):298-307
references.pmid: 10745198
references.citation: Yeast. 2005 Mar;22(4):249-70
references.pmid: 15704221
references.citation: J Ind Microbiol Biotechnol. 2007 Feb;34(2):117-22
references.pmid: 16988811
references.citation: Biotechnol Bioeng. 2004 Feb 5;85(3):330-9
references.pmid: 14748089
references.citation: FEMS Microbiol Rev. 2000 Jan;24(1):45-66
references.pmid: 10640598
references.citation: J Biotechnol. 2007 Aug 1;131(1):57-66
references.pmid: 17614154
references.citation: Methods Mol Biol. 2007;389:65-76
references.pmid: 17951635
references.citation: Protein Expr Purif. 2005 Aug;42(2):286-94
references.pmid: 15946860
references.citation: J Ind Microbiol Biotechnol. 2008 Mar;35(3):189-95
references.pmid: 18087738
references.citation: Biochem Biophys Res Commun. 1997 Oct 20;239(2):498-502
references.pmid: 9344859
references.citation: Gene. 1998 Aug 17;216(1):93-102
references.pmid: 9714758
references.citation: Clin Exp Allergy. 2007 Jun;37(6):918-28
references.pmid: 17517106
references.citation: Yeast. 1998 Jun 15;14(8):783-90
references.pmid: 9675822
references.citation: Appl Biochem Biotechnol. 2007 Aug;142(2):105-24
references.pmid: 18025573
references.citation: Cell. 1988 Mar 25;52(6):915-24
references.pmid: 3127057
references.citation: Protein Expr Purif. 2002 Jun;25(1):41-9
references.pmid: 12071697
references.citation: Yeast. 2003 Oct 15;20(13):1097-108
references.pmid: 14558143
references.citation: Biochem Biophys Res Commun. 2005 Jan 28;326(4):817-24
references.pmid: 15607743
references.citation: J Agric Food Chem. 2006 Oct 18;54(21):8041-7
references.pmid: 17032007
references.citation: Nucleic Acids Res. 1985 May 10;13(9):3043-62
references.pmid: 2987872
references.citation: Yi Chuan Xue Bao. 2004 Jun;31(6):552-7
references.pmid: 15490871
references.citation: Gene. 1997 Apr 29;190(1):55-62
references.pmid: 9185849
references.citation: Yeast. 1995 Nov;11(14):1331-44
references.pmid: 8585317
references.citation: Biochem Biophys Res Commun. 1998 Sep 18;250(2):531-5
references.pmid: 9753665
references.citation: Biotechnol Lett. 2007 Dec;29(12):1903-8
references.pmid: 17653623
references.citation: Protein Expr Purif. 2001 Oct;23(1):55-65
references.pmid: 11570846
references.citation: J Biotechnol. 2001 Jun 1;88(1):21-35
references.pmid: 11377762
references.citation: Biotechnol Bioeng. 2005 Sep 20;91(6):760-7
references.pmid: 15918169
references.citation: Protein Expr Purif. 2003 Jun;29(2):223-9
references.pmid: 12767813
references.citation: Biotechnol Bioeng. 2001 Sep 20;74(6):492-7
references.pmid: 11494216
references.citation: Nucleic Acids Res. 1987 May 11;15(9):3859-76
references.pmid: 3108861
references.citation: J Biosci Bioeng. 2008 Apr;105(4):335-40
references.pmid: 18499048
references.citation: Sheng Wu Gong Cheng Xue Bao. 2006 Jul;22(4):528-33
references.pmid: 16894882
references.citation: Virus Genes. 2006 Aug;33(1):27-32
references.pmid: 16791415
references.citation: J Biotechnol. 2007 May 31;130(1):39-46
references.pmid: 17399833
references.citation: Microb Cell Fact. 2006 Apr 06;5:17
references.pmid: 16600031
references.citation: Biotechnology (N Y). 1993 Aug;11(8):905-10
references.pmid: 7763913
references.citation: Biotechnol Bioeng. 2004 Feb 20;85(4):367-75
references.pmid: 14755554
references.citation: J Agric Food Chem. 2002 Sep 11;50(19):5313-7
references.pmid: 12207467
references.citation: Gene. 1997 Feb 20;186(1):37-44
references.pmid: 9047342
references.citation: Yeast. 2002 Jan 15;19(1):37-42
references.pmid: 11754481
references.citation: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003 Feb;35(2):127-32
references.pmid: 12545218
references.citation: J Food Sci. 2007 Mar;72(2):M67-71
references.pmid: 17995845
references.citation: Biotechnol Bioeng. 2000 Sep 5;69(5):486-94
references.pmid: 10898858
references.citation: Biotechnology (N Y). 1994 Feb;12(2):181-4
references.pmid: 7764433
references.citation: Mol Cell Biol. 1985 May;5(5):1111-21
references.pmid: 3889590
references.citation: J Mol Recognit. 2005 Mar-Apr;18(2):119-38
references.pmid: 15565717
references.citation: Biotechnology (N Y). 1991 May;9(5):455-60
references.pmid: 1367310
references.citation: Appl Microbiol Biotechnol. 2007 Mar;74(3):601-8
references.pmid: 17124582