Recombinant mussel adhesive protein as a gene delivery material.
Efficient target gene delivery into eukaryotic cells is important for biotechnological research and gene therapy. Gene delivery based on proteins, including histones, has recently emerged as a powerful non-viral DNA transfer technique. Here, we investigated the potential use of a recombinant mussel adhesive protein, hybrid fp-151, as a gene delivery material, in view of its similar basic amino acid composition to histone proteins, and cost-effective and high-level production in Escherichia coli. After confirming DNA binding affinity, we transfected mammalian cells (human 293T and mouse NIH/3T3) with foreign genes using hybrid fp-151 as the gene delivery carrier. Hybrid fp-151 displayed comparable transfection efficiency in both mammalian cell lines, compared to the widely used transfection agent, Lipofectamine 2000. Our results indicate that this mussel adhesive protein may be used as a potential protein-based gene-transfer mediator.
Hwang
Dong Soo
DS
National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Kim
Kyoung Ro
KR
Lim
Seonghye
S
Choi
Yoo Seong
YS
Cha
Hyung Joon
HJ
D000818:Animals; D004247:DNA / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D009154:Mutation; D041681:NIH 3T3 Cells; D011485:Protein Binding; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity; D014162:Transfection
D011506:Proteins; D011994:Recombinant Proteins; C037403:adhesive protein, mussel; D004247:DNA