Reprogramming primordial germ cells (PGC) to embryonic germ (EG) cells.
In this unit we describe the derivation of pluripotent embryonic germ (EG) cells from mouse primordial germ cells (PGCs) isolated from both 8.5- and 11.5-days post-coitum (dpc) embryos. Once EG cells are derived we explain how to propagate and characterize the cell lines. We introduce readers to PGCs and explain differences between PGCs and their in vitro derivatives EG cells. Finally, we also compare mouse EG cells with ES cells. This unit will be of great interest to anyone interested in PGCs or studying the behavior of cultured PGCs or the derivation of new EG cell lines.
Durcova-Hills
Gabriela
G
The Wellcome Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, Cambridge, United Kingdom.
Surani
Azim
A
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002469:Cell Separation; D065150:Cellular Reprogramming; D015925:Cryopreservation; D004622:Embryo, Mammalian / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D005854:Germ Cells / Q000166:cytology*; D016256:Lewis X Antigen / Q000378:metabolism; D008280:Magnetics; D051379:Mice; D008863:Microspheres; D008938:Mitosis; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis; D013234:Stem Cells / Q000166:cytology*
D016256:Lewis X Antigen; D050814:Octamer Transcription Factor-3; D000469:Alkaline Phosphatase