Cell cycle analysis of budding yeast using SYTOX Green.
Flow cytometric determination of DNA content is a standard tool for cell cycle analysis in the budding yeast, Saccharomyces cerevisiae. Using SYTOX Green instead of propidium iodide (PI) in a standard ethanol fixation protocol increases the accuracy and reproducibility of cell cycle analyses. SYTOX-stained cells exhibit improved coefficients of variation (CVs), a better correlation between DNA content and fluorescence, and decreased sensitivity to variances in experimental conditions when compared with PI-stained cells. The SYTOX Green protocol provides researchers with a more accurate and reliable alternative to standard PI-based protocols for the analysis of the budding yeast cell cycle.
Haase
Steven B
SB
Duke University, Durham, North Carolina, USA.
D002453:Cell Cycle; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods; D009930:Organic Chemicals; D004718:Saccharomycetales / Q000166:cytology*
D007202:Indicators and Reagents; D009930:Organic Chemicals; C402795:SYTOX Green; D004247:DNA