Diurnal oscillation of SBE expression in sorghum endosperm.
Spatial and temporal expression patterns of the sorghum SBEI, SBEIIA and SBEIIB genes, encoding, respectively, starch branching enzyme (SBE) I, IIA and IIB, in the developing endosperm of sorghum (Sorghum bicolor) were studied. Full-length genomic and cDNA clones for sorghum were cloned, and the SBEIIA cDNA was used together with gene-specific probes for sorghum SBEIIB and SBEI. In contrast to sorghum SBEIIB, which was expressed primarily in endosperm and embryo, SBEIIA was also expressed in vegetative tissues. All three genes shared a similar temporal expression profile during endosperm development, with a maximum activity at 15-24 d after pollination. This differed from barley and maize, in which SBEI gene activity showed a significantly later onset compared to that of SBEIIA and SBEIIB. Expression of the three SBE genes in the sorghum endosperm exhibited a diurnal rhythm during a 24-h cycle.
Mutisya
Joel
J
Department of Plant Biology & Forest Genetics, Uppsala BioCenter, The Swedish University of Agricultural Sciences, Uppsala, Sweden.
Sun
Chuanxin
C
Rosenquist
Sara
S
Baguma
Yona
Y
Jansson
Christer
C
D015061:1,4-alpha-Glucan Branching Enzyme / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D002940:Circadian Rhythm / Q000235:genetics; D018076:DNA, Complementary / Q000302:isolation & purification; D018744:DNA, Plant / Q000302:isolation & purification; D018628:Gene Dosage; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D012639:Seeds / Q000201:enzymology* / Q000235:genetics*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D045868:Sorghum / Q000201:enzymology* / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; D015061:1,4-alpha-Glucan Branching Enzyme