title: Confocal diffraction phase microscopy of live cells.
abstract: We present a new quantitative phase microscopy technique, confocal diffraction phase microscopy, which provides quantitative phase measurements from localized sites on a sample with high sensitivity. The technique combines common-path interferometry with confocal microscopy in a transmission geometry. The capability of the technique for static imaging is demonstrated by imaging polystyrene microspheres and live HT29 cells, while dynamic imaging is demonstrated by quantifying the nanometer scale fluctuations of red blood cell membranes.
authors.affiliation: G.H. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Niyom
authors.initials: N
authors.lastname: Lue
authors.forename: Wonshik
authors.initials: W
authors.lastname: Choi
authors.forename: Kamran
authors.initials: K
authors.lastname: Badizadegan
authors.forename: Ramachandra R
authors.initials: RR
authors.lastname: Dasari
authors.forename: Michael S
authors.initials: MS
authors.lastname: Feld
authors.forename: Gabriel
authors.initials: G
authors.lastname: Popescu
mesh_terms: D002460:Cell Line; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D012542:Scattering, Radiation
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