title: Strategies for shotgun identification of integral membrane proteins by tandem mass spectrometry.
abstract: Integral membrane proteins (IMPs) are difficult to identify, mainly for two reasons: the hydrophobicity of IMPs and their low abundance. Sample preparation is a key component in the large-scale identification of IMPs. In this review, we survey strategies for shotgun identification of IMPs by MS/MS. We will discuss enrichment, solubilization, separation, and digestion of IMPs, and data analysis for membrane proteomics.
authors.affiliation: Department of Chemical Physiology, SR-11, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Bingwen
authors.initials: B
authors.lastname: Lu
authors.forename: Daniel B
authors.initials: DB
authors.lastname: McClatchy
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
mesh_terms: D000818:Animals; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D008565:Membrane Proteins