Salidroside inhibits H2O2-induced apoptosis in PC12 cells by preventing cytochrome c release and inactivating of caspase cascade.
We used a rat pheochromocytoma (PC12) cell line to study the effects of salidroside on hydrogen peroxide (H(2)O(2))-induced apoptosis. In PC12 cells, H(2)O(2)-induced apoptosis was accompanied by the down-regulation of Bcl-2, the up-regulation of Bax, the release of mitochondrial cytochrome c to cytosol, and the activation of caspase-3, -8 and -9. However, salidroside suppressed the down-regulation of Bcl-2, the up-regulation of Bax and the release of mitochondrial cytochrome c to cytosol. Moreover, salidroside attenuated caspase-3, -8 and -9 activation, and eventually protected cells against H(2)O(2)-induced apoptosis. Taken together, these results suggest that treatment of PC12 cells with salidroside can block H(2)O(2)-induced apoptosis by regulating Bcl-2 family members and by suppressing cytochrome c release and caspase cascade activation.
Cai
Lei
L
School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
Wang
Hua
H
Li
Qin
Q
Qian
Yunfei
Y
Yao
Wenbing
W
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D006690:Bisbenzimidazole / Q000378:metabolism; D020169:Caspases / Q000032:analysis / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism*; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005456:Fluorescent Dyes / Q000378:metabolism; D005562:Formazans / Q000032:analysis / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D016716:PC12 Cells; D010636:Phenols / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013778:Tetrazolium Salts / Q000032:analysis / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D005456:Fluorescent Dyes; D005562:Formazans; D005960:Glucosides; D010636:Phenols; D020011:Protective Agents; D012333:RNA, Messenger; D013778:Tetrazolium Salts; C079782:MTT formazan; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D020169:Caspases; D006690:Bisbenzimidazole; C009172:rhodioloside