Frequent emergence and limited geographic dispersal of methicillin-resistant Staphylococcus aureus.
A small number of clonal lineages dominates the global population structure of methicillin-resistant Staphylococcus aureus (MRSA), resulting in the concept that MRSA has emerged on a few occasions after penicillinase-stable beta-lactam antibiotics were introduced to clinical practice, followed by intercontinental spread of individual clones. We investigated the evolutionary history of an MRSA clone (ST5) by mutation discovery at 108 loci (46 kb) within a global collection of 135 isolates. The SNPs that were ascertained define a radial phylogenetic structure within ST5 consisting of at least 5 chains of mutational steps that define geographically associated clades. These clades are not concordant with previously described groupings based on staphylococcal protein A gene (spa) typing. By mapping the number of independent imports of the staphylococcal cassette chromosome methicillin-resistance island, we also show that import has occurred on at least 23 occasions within this single sequence type and that the progeny of such recombinant strains usually are distributed locally rather than globally. These results provide strong evidence that geographical spread of MRSA over long distances and across cultural borders is a rare event compared with the frequency with which the staphylococcal cassette chromosome island has been imported.
Nübel
Ulrich
U
Robert Koch Institut, 38855 Wernigerode, Germany. nuebelu@rki.de
Roumagnac
Philippe
P
Feldkamp
Mirjam
M
Song
Jae-Hoon
JH
Ko
Kwan Soo
KS
Huang
Yhu-Chering
YC
Coombs
Geoffrey
G
Ip
Margaret
M
Westh
Henrik
H
Skov
Robert
R
Struelens
Marc J
MJ
Goering
Richard V
RV
Strommenger
Birgit
B
Weller
Annette
A
Witte
Wolfgang
W
Achtman
Mark
M
D006239:Haplotypes; D016106:Methicillin Resistance / Q000235:genetics; D010802:Phylogeny; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics
Pediatrics. 1999 Apr;103(4):e39
10103331
Clin Microbiol Infect. 2006 Dec;12(12):1175-84
17121623
Annu Rev Microbiol. 2008;62:53-70
18785837
Nat Rev Microbiol. 2006 Dec;4(12):953-8
17109032
J Clin Microbiol. 2008 Feb;46(2):574-81
18032612
Trends Microbiol. 2001 Oct;9(10):486-93
11597450
Nat Genet. 2008 Aug;40(8):987-93
18660809
Antimicrob Agents Chemother. 2006 Oct;50(10):3457-9
17005831
Semin Respir Crit Care Med. 2003 Feb;24(1):121-34
16088531
Infect Genet Evol. 2004 Sep;4(3):205-13
15450200
Infection. 1994;22 Suppl 2:S128-34
7927831
Lancet. 2006 Sep 2;368(9538):874-85
16950365
FEMS Immunol Med Microbiol. 2006 Feb;46(1):8-20
16420592
Emerg Infect Dis. 2004 Sep;10(9):1568-77
15498158
Science. 2006 Nov 24;314(5803):1301-4
17124322
J Clin Microbiol. 2000 Mar;38(3):1008-15
10698988
Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9865-70
11481426
Trends Microbiol. 2006 Aug;14(8):353-5
16782339
Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9786-91
15213324
Emerg Infect Dis. 2001 Mar-Apr;7(2):178-82
11294701
Nat Rev Immunol. 2006 Jun;6(6):465-75
16724100
J Infect Dis. 2006 Jan 15;193(2):172-9
16362880
PLoS Biol. 2005 Oct;3(10):e335
16144426
Emerg Infect Dis. 2007 Dec;13(12):1840-6
18258033
J Antimicrob Chemother. 2007 Mar;59(3):465-72
17289763
Lancet. 2001 Apr 21;357(9264):1225-40
11418146
Antimicrob Agents Chemother. 2006 Oct;50(10):3237-44
17005800
Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13536-41
15347815
J Bacteriol. 2003 Jun;185(11):3392-9
12754238
Antimicrob Agents Chemother. 2006 Mar;50(3):1001-12
16495263
Lancet Infect Dis. 2005 Dec;5(12):751-62
16310147
Antimicrob Agents Chemother. 2007 May;51(5):1671-7
17307983
Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14043-8
10570195
Science. 2006 Jun 30;312(5782):1944-6
16809538
Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17837-42
15598742
J Clin Microbiol. 2003 Apr;41(4):1574-85
12682148
Int J Med Microbiol. 2008 Jul;298(5-6):365-77
18325835
Crit Care Med. 1999 May;27(5):887-92
10362409
J Clin Microbiol. 2008 May;46(5):1741-6
18322069
Emerg Infect Dis. 2008 Apr;14(4):653-6
18394287
Antimicrob Agents Chemother. 2003 Dec;47(12):3926-34
14638503