Bivariate analysis of DNA content and expression of cyclin proteins.
Cyclins are key components of the cell cycle machinery. This unit describes techniques associated with measurement of these transiently expressed molecules and focusses on the combination of cell cycle analysis and expression of proliferation-associated proteins via immunocytochemically attached antibodies.
Juan
G
G
New York Medical College, Valhalla, New York, USA.
Darzynkiewicz
Z
Z
D000818:Animals; D002453:Cell Cycle; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D016213:Cyclins / Q000096:biosynthesis*; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007700:Kinetics; D051379:Mice; D013223:Statistics as Topic; D013997:Time Factors
D016213:Cyclins; D004247:DNA