Expression of anaphase-promoting complex7 in fibroadenomas and phyllodes tumors of breast.
The proteolytic destruction of cyclin B is an important event during cell division. Cyclin B proteolysis is triggered by the anaphase-promoting complex. Therefore, cell cycle dysregulation due to anaphase-promoting complex loss contributes to cell transformation and human carcinogenesis. This study investigates anaphase-promoting complex7 expression in spindle cell breast tumors and also includes a comparison between the proliferation antigen Ki-67 and S-phase fraction. The average values of the anaphase-promoting complex7 and Ki-67 labeling indices increased in order from benign to malignant within the phyllodes tumor group, and the fibroadenoma and juvenile fibroadenoma exhibited lower levels of anaphase-promoting complex7 and Ki-67 expression than did the phyllodes tumor. The frequency of anaphase-promoting complex7-positive stromal cells correlated with Ki-67 expression in phyllodes tumor and in all of the examined breast tumors. The above results indicate that anaphase-promoting complex7 and Ki-67 are closely related to cell proliferation. In addition, phyllodes tumor can be differentiated from juvenile fibroadenoma with increased mitotic figures mimicking phyllodes tumor by anaphase-promoting complex7 and Ki-67 immunochemistry. Because anaphase-promoting complex7 is expressed at higher levels than is Ki-67, it may overcome the limitations of the Ki-67 labeling index with regard to the differentiation of benign phyllodes tumor from fibroadenoma.
Kang
Yup
Y
Institute for Medical Science, Ajou University School of Medicine, Suwon 443-721, Korea.
Kim
Jang Hee
JH
Lee
Tae Hui
TH
Kim
Tai Seung
TS
Jung
Woo Hee
WH
Chung
Hyun Cheol
HC
Park
Byeong Woo
BW
Sheen
Seung Soo
SS
Han
Jae Ho
JH
D000293:Adolescent; D000328:Adult; D064173:Anaphase-Promoting Complex-Cyclosome; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D018226:Fibroadenoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008875:Middle Aged; D003557:Phyllodes Tumor / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012189:Retrospective Studies; D016196:S Phase; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
D019394:Ki-67 Antigen; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome