Analysis of DNA content and BrdU incorporation.
During the past two decades, the techniques of analysis of cell cycle kinetics based upon the flow cytometric detection of BrdU incorporation have replaced the cumbersome autoradiographic methodology utilizing tritiated thymidine. This unit distills the knowledge gained over this time into concise and easy to follow protocols for DNA content analysis following thermal denaturation or acid denaturation, as well as a modified method that combines thermal denaturation with washless immunocytochemical detection of BrdU.
Darzynkiewicz
Z
Z
New York Medical College, Valhalla, New York, USA.
Juan
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G
D000818:Animals; D001973:Bromodeoxyuridine / Q000032:analysis* / Q000378:metabolism; D002453:Cell Cycle; D002469:Cell Separation / Q000379:methods*; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007700:Kinetics; D011003:Ploidies; D013936:Thymidine / Q000032:analysis
D004247:DNA; D001973:Bromodeoxyuridine; D013936:Thymidine