title: BAC-derived markers converted from RFLP linked to Phytophthora capsici resistance in pepper (Capsicum annuum L.).
abstract: Phytophthora capsici Leonian, an oomycete pathogen, is a serious problem in pepper worldwide. Its resistance in pepper is controlled by quantitative trait loci (QTL). To detect QTL associated with P. capsici resistance, a molecular linkage map was constructed using 100 F(2) individuals from a cross between Capsicum annuum 'CM334' and C. annuum 'Chilsungcho'. This linkage map consisted of 202 restriction fragment length polymorphisms (RFLPs), 6 WRKYs and 1 simple sequence repeat (SSR) covering 1482.3 cM, with an average interval marker distance of 7.09 cM. QTL mapping of Phytophthora root rot and damping-off resistance was performed in F(2:3) originated from a cross between resistant Mexican landrace C. annuum 'CM334' and susceptible Korean landrace C. annuum 'Chilsungcho' using composite interval mapping (CIM) analysis. Four QTL explained 66.3% of the total phenotypic variations for root rot resistance and three 44.9% for damping-off resistance. Of these QTL loci, two were located close to RFLP markers CDI25 on chromosome 5 (P5) and CT211A on P9. A bacterial artificial chromosome (BAC) library from C. annuum 'CM334' was screened with these two RFLP probes to obtain sequence information around the RFLP marker loci for development of PCR-based markers. CDI25 and CT211 probes identified seven and eight BAC clones, respectively. Nine positive BAC clones containing probe regions were sequenced and used for cytogenetic analysis. One single-nucleotide amplified polymorphism (SNAP) for the CDI25 locus, and two SSRs and cleaved amplified polymorphic sequence (CAPS) for CT211 were developed using sequences of the positive BAC clones. These markers will be valuable for rapid selection of genotypes and map-based cloning for resistance genes against P. capsici.
authors.affiliation: Department of Plant Science, Seoul National University, Seoul, 151-921, South Korea.
authors.forename: Hyoun-Joung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Seok-Hyeon
authors.initials: SH
authors.lastname: Nahm
authors.forename: Heung-Ryul
authors.initials: HR
authors.lastname: Lee
authors.forename: Gi-Bo
authors.initials: GB
authors.lastname: Yoon
authors.forename: Ki-Taek
authors.initials: KT
authors.lastname: Kim
authors.forename: Byoung-Cheorl
authors.initials: BC
authors.lastname: Kang
authors.forename: Doil
authors.initials: D
authors.lastname: Choi
authors.forename: Oh Yeol
authors.initials: OY
authors.lastname: Kweon
authors.forename: Myeong-Cheoul
authors.initials: MC
authors.lastname: Cho
authors.forename: Jin-Kyung
authors.initials: JK
authors.lastname: Kwon
authors.forename: Jung-Heon
authors.initials: JH
authors.lastname: Han
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Minkyu
authors.initials: M
authors.lastname: Park
authors.forename: Jong Hwa
authors.initials: JH
authors.lastname: Ahn
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Nam Han
authors.initials: NH
authors.lastname: Her
authors.forename: Joo-Hee
authors.initials: JH
authors.lastname: Sung
authors.forename: Byung-Dong
authors.initials: BD
authors.lastname: Kim
mesh_terms: D002212:Capsicum / Q000235:genetics* / Q000382:microbiology; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant; D007113:Immunity, Innate; D010838:Phytophthora / Q000472:pathogenicity; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
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