title: Molecular imaging of proteins in tissues by mass spectrometry.
abstract: Imaging MS (IMS) is an emerging technology that permits the direct analysis and determination of the distribution of molecules in tissue sections. Biological molecules such as proteins, peptides, lipids, xenobiotics, and metabolites can be analyzed in a high-throughput manner with molecular specificity not readily achievable through other means. Tissues are analyzed intact and thus spatial localization of molecules within a tissue is preserved. Several studies are presented that focus on the unique types of information obtainable by IMS, such as Abeta isoform distributions in Alzheimer's plaques, protein maps in mouse brain, and spatial protein distributions in human breast carcinoma. The analysis of a biopsy taken 100 years ago from a patient with amyloidosis illustrates the use of IMS with formalin-fixed tissues. Finally, the registration and correlation of IMS with MRI is presented.
authors.affiliation: Mass Spectrometry Research Center and Department of Biochemistry, Vanderbilt University School of Medicine, 465 21st Avenue South, MRB III Suite 9160, Nashville, TN 37232, USA.
authors.forename: Erin H
authors.initials: EH
authors.lastname: Seeley
authors.forename: Richard M
authors.initials: RM
authors.lastname: Caprioli
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D008279:Magnetic Resonance Imaging; D011506:Proteins / Q000032:analysis*; D013048:Specimen Handling; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D011506:Proteins
references.citation: Anal Chem. 1991 Dec 15;63(24):1193A-1203A
references.pmid: 1789447
references.citation: Nat Methods. 2008 Jan;5(1):101-8
references.pmid: 18165806
references.citation: Anal Chem. 2006 Sep 15;78(18):6448-56
references.pmid: 16970320
references.citation: Anal Chem. 2005 Aug 1;77(15):5036-40
references.pmid: 16053319
references.citation: Arch Pathol Lab Med. 1984 Mar;108(3):217-9
references.pmid: 6198999
references.citation: Anal Chem. 2007 Sep 1;79(17):6862-7
references.pmid: 17658766
references.citation: Endocrinology. 2008 Jul;149(7):3274-8
references.pmid: 18403475
references.citation: Anal Chem. 1997 Dec 1;69(23):4751-60
references.pmid: 9406525
references.citation: Cancer Res. 2004 Dec 15;64(24):9093-100
references.pmid: 15604278
references.citation: J Am Soc Mass Spectrom. 2007 Mar;18(3):567-77
references.pmid: 17157526
references.citation: Mol Cell Proteomics. 2006 Oct;5(10):1975-83
references.pmid: 16849436
references.citation: J Mass Spectrom. 2007 Feb;42(2):254-62
references.pmid: 17230433
references.citation: J Mass Spectrom. 2003 Jul;38(7):699-708
references.pmid: 12898649
references.citation: Mol Cell Proteomics. 2006 Oct;5(10):1876-86
references.pmid: 16705188
references.citation: Mass Spectrom Rev. 2007 Jul-Aug;26(4):606-43
references.pmid: 17471576
references.citation: Int J Mass Spectrom. 2007 Feb 1;260(2-3):212-221
references.pmid: 17541451
references.citation: Cancer Res. 2005 Sep 1;65(17):7674-81
references.pmid: 16140934
references.citation: J Am Soc Mass Spectrom. 2003 Jul;14(7):752-9
references.pmid: 12837597
references.citation: Int J Mol Med. 2007 Aug;20(2):155-9
references.pmid: 17611632
references.citation: Clin Cancer Res. 2006 Sep 1;12(17):5142-50
references.pmid: 16951232
references.citation: Anal Chem. 2004 Feb 15;76(4):1145-55
references.pmid: 14961749
references.citation: Anal Chem. 2006 Feb 1;78(3):827-34
references.pmid: 16448057
references.citation: Rev Sci Instrum. 2007 May;78(5):053716
references.pmid: 17552834
references.citation: Am J Respir Crit Care Med. 2005 Dec 15;172(12):1556-62
references.pmid: 16179643
references.citation: Chem Rev. 1999 Oct 13;99(10):2977-3006
references.pmid: 11749508
references.citation: Nat Methods. 2008 Jan;5(1):57-9
references.pmid: 18084298
references.citation: Nat Med. 2001 Apr;7(4):493-6
references.pmid: 11283679
references.citation: J Proteome Res. 2007 Nov;6(11):4127-34
references.pmid: 17939699
references.citation: J Am Soc Mass Spectrom. 2008 Aug;19(8):1069-77
references.pmid: 18472274
references.citation: Rapid Commun Mass Spectrom. 2006;20(2):284-90
references.pmid: 16345125
references.citation: Proteomics. 2003 Nov;3(11):2221-39
references.pmid: 14595821
references.citation: Mol Cell Proteomics. 2008 Feb;7(2):411-23
references.pmid: 17991918