Structure determination of beta-glucans from Ganoderma lucidum with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.
A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, beta-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lucidum, dextran, curdlan and maltohexaose were also pursued and different fragment patterns were obtained. The G. lucidum glucan structure was determined and this method for linkage analysis of permethylated glucan has been proven feasible.
Hung
Wei-Ting
WT
Genomics Research Center, Academia Sinica, No. 128, Academia Road Section 2, Nan-Kang, Taipei 11529, Taiwan.
Wang
Shwu-Huey
SH
Chen
Chung-Hsuan
CH
Yang
Wen-Bin
WB
D002240:Carbohydrate Sequence; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005936:Glucans / Q000737:chemistry*; D008745:Methylation; D008970:Molecular Weight; D020940:Reishi / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D005936:Glucans
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