Experimental approaches for solution X-ray scattering and fiber diffraction.
X-ray scattering and diffraction from non-crystalline systems have gained renewed interest in recent years, as focus shifts from the structural chemistry information gained by high-resolution studies to the context of structural physiology at larger length scales. Such techniques permit the study of isolated macromolecules as well as highly organized macromolecular assemblies as a whole under near-physiological conditions. Time-resolved approaches, made possible by advanced synchrotron instrumentation, add a crucial dimension to many of these investigations. This article reviews experimental approaches in non-crystalline X-ray scattering and diffraction that may be used to illuminate important scientific questions such as protein/nucleic acid folding and structure-function relationships in large macromolecular assemblies.
Tsuruta
H
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Stanford Synchrotron Radiation Laboratory (SSRL), SLAC, Stanford University, MS69, Menlo Park, CA 94025, USA. tsuruta@stanford.edu
Irving
T C
TC
D000818:Animals; D003238:Connective Tissue / Q000648:ultrastructure; D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods; D004330:Drosophila; D008958:Models, Molecular; D008968:Molecular Conformation; D009502:Neutrons; D009696:Nucleic Acids / Q000737:chemistry*; D019999:Pharmaceutical Solutions; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012542:Scattering, Radiation; D012996:Solutions; D017356:Synchrotrons; D014961:X-Ray Diffraction / Q000295:instrumentation* / Q000379:methods
D009696:Nucleic Acids; D019999:Pharmaceutical Solutions; D011506:Proteins; D012996:Solutions
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