Whole blood analysis of leukocyte-platelet aggregates.
In inflammatory and thrombotic syndromes, platelets aggregate with circulating leukocytes, especially monocytes and neutrophils. The platelet binding is initiated primarily through platelet surface expression of P-selectin (CD62P) following activation-dependent degranulation. The levels of P-selectin involved can be low enough to make direct measurement difficult, but detection of leukocyte-platelet aggregates is relatively simply by whole-blood flow cytometry. Light scatter and at least one leukocyte-specific antibody are used to gate the desired population, and the presence of associated platelets is detected by immunostaining for abundant platelet-specific markers.
Barnard
Marc R
MR
University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Krueger
Lori A
LA
Frelinger
A L
AL
Furman
Mark I
MI
Michelson
Alan D
AD
D000818:Animals; D000911:Antibodies, Monoclonal; D001769:Blood; D001792:Blood Platelets / Q000166:cytology* / Q000276:immunology; D002448:Cell Adhesion; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology; D008722:Methods
D000911:Antibodies, Monoclonal