title: Spectrofluorimetric determination of vitamin B(1) using horseradish peroxidase as catalyst in the presence of hydrogen peroxide.
abstract: In this work a new spectrofluorimetric method for the determination of vitamin B(1), based on the catalytic activity of horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H2O2), has been developed. Non-fluorescent vitamin B(1) was easily converted through catalytic oxidation in alkaline medium into a fluorescent compound, even without exposure to light. The linear range for vitamin B(1) observed was 0.026-16.83 microg/mL (RSD = 1.75%). The correlation coefficient for the calibration curve and limit of detection were found to be 0.9964 and 0.015 microg/mL, respectively. The developed method is practical, simple, sensitive and relatively free from interference by coexisting substances and has been successfully applied for the determination of vitamin B(1) in pharmaceutical preparations.
authors.affiliation: Department of Chemistry, Kyungpook National University, Taegu 702-701, South Korea.
authors.forename: Mir Azam
authors.initials: MA
authors.lastname: Khan
authors.forename: Seung Oh
authors.initials: SO
authors.lastname: Jin
authors.forename: Sang Hak
authors.initials: SH
authors.lastname: Lee
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Chung
mesh_terms: D002138:Calibration; D002384:Catalysis; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013831:Thiamine / Q000032:analysis*
chemical_list: D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D013831:Thiamine