title: Indices of lower airway inflammation in children monosensitized to house dust mite after nasal allergen challenge.
abstract: BACKGROUND
  There are few available data assessing the united airway disease and its systemic aspects in children. With this study, we aimed to investigate the inflammation markers of upper and lower airways before and after nasal allergen challenge in mite sensitive children with different clinical expression of the allergic disease.
  
  
  METHODS
  Four study groups were formed: rhinitis only, without bronchial hyper-responsiveness (R, n = 10), rhinitis with asthma (R + A, n = 22), atopic asymptomatics (AA, n = 8) and nonallergic healthy controls (C, n = 10). Blood eosinophils, nasal and sputum eosinophils, sputum eosinophil cationic protein (ECP) and cys-LTs, and serum ECP levels were measured before and 24 h after nasal allergen challenge.
  
  
  RESULTS
  The groups were comparable in terms of age and gender. Cumulative symptom scores recorded during and 1 h after nasal challenge were not significantly different between patients with R, R + A and AA groups. At T(24), the children belonging to R, R + A and AA showed significant increases in nasal eosinophils (P < 0.01, P < 0.001, and P = 0.01, respectively), sputum eosinophils (P = 0.01, P < 0.001, and P < 0.05, respectively) and blood eosinophils (P < 0.01, P < 0.001, and P < 0.05, respectively). Similarly, increases in sputum ECP (P < 0.01, P < 0.001, and P = 0.07, respectively) and sputum cys-LT levels (P = 0.07, P < 0.001, and P < 0.05, respectively) were detected in children belonging to these three groups at T(24). Sputum eosinophils significantly correlated with blood eosinophils (r = 0.54, P < 0.001) and sputum ECP (r = 0.58, P < 0.001) at T(24).
  
  
  CONCLUSIONS
  This study showed that nasal allergen challenge increased markers of eosinophilic inflammation in both upper and lower airways of children monosensitized to mites, even before the onset of clinical symptoms.
authors.affiliation: Division of Pediatric Allergy and Immunology, Faculty of Medicine, University of Cukurova, Adana, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Inal
authors.forename: S G
authors.initials: SG
authors.lastname: Kendirli
authors.forename: M
authors.initials: M
authors.lastname: Yilmaz
authors.forename: D U
authors.initials: DU
authors.lastname: Altintas
authors.forename: G B
authors.initials: GB
authors.lastname: Karakoc
authors.forename: S
authors.initials: S
authors.lastname: Erdogan
mesh_terms: D000293:Adolescent; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage* / Q000097:blood / Q000276:immunology*; D001249:Asthma / Q000175:diagnosis / Q000276:immunology / Q000473:pathology*; D016535:Bronchial Hyperreactivity / Q000175:diagnosis / Q000276:immunology / Q000473:pathology*; D002648:Child; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D004804:Eosinophils / Q000473:pathology; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000276:immunology / Q000473:pathology*; D015289:Leukotrienes / Q000378:metabolism; D008297:Male; D009299:Nasal Provocation Tests; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis / Q000276:immunology / Q000473:pathology*; D012882:Skin Tests; D013183:Sputum / Q000276:immunology / Q000378:metabolism
chemical_list: D039741:Antigens, Dermatophagoides; D015289:Leukotrienes