title: [Primary evaluation of anti-HEV diagnostic reagent by experimental infection animal model with hepatitis E virus].
abstract: OBJECTIVE
  To evaluate anti-HEV diagnostic kits by experimental infecting rhesus monkeys with HEV.
  
  
  METHODS
  Eight rhesus monkeys were infected with genotype 1 and 4 HEV separately. The alanine aminotransferase (ALT) level of all monkeys were detected before and after the process of infection. HEV RNA in stool specimens was tested by reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Anti-HEV IgG in serum was detected by GL-IgG and WT-IgG.
  
  
  RESULTS
  HEV RNA presented in the stool of all the 8 monkeys after infection. The ALT level of 1 monkey infected with genotype 1 HEV and 2 monkeys infected with genotype 4 HEV appeared abnormally after infection. Tested by GL-IgG, 2 of the 4 monkeys infected with genotype 1 HEV and 1 of 4 monkeys infected with genotype 4 HEV seroconverted to anti-HEV IgG. However, when tested by WT-IgG, all the infected monkeys seroconverted to anti-HEV IgG. The anti-HEV IgG tested by WT-IgG was positive during the whole observation period,and the anti-HEV IgG measured by GL-IgG only remained 12 weeks after infection. Detected by GL-IgG and WT-IgG, seropositive conversion of the anti-HEV IgG happened almost at the same time.
  
  
  CONCLUSIONS
  Both GL-IgG and WT-IgG could detect the anti-HEV IgG of experimentally infected rhesus monkeys but the WT-IgG had a higher sensitivity for detection of anti-HEV IgG than
authors.affiliation: National Institute for the Control of Pharmaceutical and Biological Product, Beijing 100050, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhou
authors.forename: Wei-jin
authors.initials: WJ
authors.lastname: Huang
authors.forename: Xing
authors.initials: X
authors.lastname: Wu
authors.forename: Hai-yun
authors.initials: HY
authors.lastname: Lan
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Gu
authors.forename: Guo-yong
authors.initials: GY
authors.lastname: Huang
authors.forename: Hua-yuan
authors.initials: HY
authors.lastname: Zhang
authors.forename: Zi-bai
authors.initials: ZB
authors.lastname: Qi
authors.forename: He-min
authors.initials: HM
authors.lastname: Li
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D005838:Genotype; D016751:Hepatitis E / Q000276:immunology* / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D008253:Macaca mulatta; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007074:Immunoglobulin G; D000410:Alanine Transaminase