Cysteine-rich 61 (CYR61) inhibits cisplatin-induced apoptosis in ovarian carcinoma cells.
Cysteine-rich 61 (CYR61), a member of the connective tissue factor CCN (Cyr61, CTGF, Nov) family, facilitates angiogenesis by interacting with integrins. Recent observations have indicated that CYR61 also rescues cells from anti-cancer drug-mediated apoptosis but the detailed mechanism underlying the role of CYR61 during apoptosis has not been identified. To better understand the role of CYR61 during cisplatin-induced apoptosis in tumor cells, we overexpressed or inhibited CYR61 expression in human cervical cancer cells (HeLa cells) and measured cisplatin-mediated apoptosis. The results from these experiments clearly demonstrate that CYR61 prevents cisplatin-induced apoptosis by inhibiting caspase-3 activity in HeLa cells. Therefore, CYR61 may be a useful therapeutic target for cisplatin-resistant tumors.
Rho
Seung Bae
SB
Research Institute, National Cancer Center, 809, Madu 1-dong, Ilsan-gu, Goyang-si, Gyeonggi-do 411-769, Republic of Korea.
Woo
Jong Soo
JS
Byun
Hyun-Jung
HJ
Chun
Taehoon
T
Park
Sang-Yoon
SY
D017209:Apoptosis / Q000187:drug effects*; D061945:Caspase Inhibitors; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D055515:Cysteine-Rich Protein 61 / Q000378:metabolism*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D010051:Ovarian Neoplasms / Q000201:enzymology / Q000473:pathology*; D034741:RNA, Small Interfering / Q000378:metabolism
C501848:CCN1 protein, human; D061945:Caspase Inhibitors; D055515:Cysteine-Rich Protein 61; D034741:RNA, Small Interfering; D002945:Cisplatin