Ovine progressive pneumonia provirus levels associate with breed and Ovar-DRB1.
Previous studies initiated defining the role of host genetics in influencing the outcome of exposure to ovine progressive pneumonia virus. However, specific genes influencing host control of virus replication and disease progression have not been identified. This study, using 383 ewes of the Columbia, Polypay, and Rambouillet breeds, tested the hypothesis that host control of OPPV as measured by provirus levels in the peripheral blood associates with certain breeds and MHC class II Ovis aries (Ovar)-DRB1 expressed alleles. Rambouillet ewes were less likely to have measurable provirus levels as compared to Columbia ewes at ages 5 and 6 (P value < 0.02), and they exhibited lower provirus levels when compared to both Columbia and Polypay ewes of the same ages (P value < 0.05). The presence of DRB1*0403- or DRB1*07012-expressed alleles were significantly associated (P value = 0.019 and 0.0002, respectively) with lower OPP provirus levels but only were only found in 11% of the ewe flock. Analysis of each segregating amino acid in the beta1 domain of DR beta-chain revealed that amino acids Y31, T32, N37, T51, Q60, or N74 significantly associated (P value range = 0.0003-0.018) with lower OPP provirus levels, whereas amino acids H32, A38, or I67 associated (P value range = 0.013-0.043) with higher OPP provirus levels. These results suggest that Ovar-DRB1 contributes as one host genetic factor that controls OPP provirus levels, but does not fully account for the breed-specific OPP proviral differences.
Herrmann-Hoesing
Lynn M
LM
US Department of Agriculture, Animal Disease Research Unit, Agricultural Research Service, Pullman, WA 99164-6630, USA. lherrman@vetmed.wsu.edu
White
Stephen N
SN
Mousel
Michelle R
MR
Lewis
Gregory S
GS
Knowles
Donald P
DP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D005787:Gene Frequency; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D054884:Host-Pathogen Interactions; D010802:Phylogeny; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D011533:Proviruses / Q000302:isolation & purification*; D016415:Sequence Alignment; D017385:Sequence Homology; D012756:Sheep / Q000235:genetics* / Q000276:immunology; D012757:Sheep Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D013045:Species Specificity; D019562:Viral Load; D014766:Viremia / Q000235:genetics / Q000276:immunology; D016662:Virus Integration; D014779:Virus Replication; D014790:Visna-maedi virus / Q000302:isolation & purification*
D000949:Histocompatibility Antigens Class II
Immunogenetics. 2008 Apr;60(3-4):167-76
18322680
Annu Rev Med. 2003;54:535-51
12525683
Eur J Immunogenet. 2003 Aug;30(4):275-82
12919289
Ann Neurol. 1988;23 Suppl:S95-100
2831808
Anim Genet. 1994 Aug;25(4):235-41
7527194
Adv Virus Res. 1988;34:189-215
2843016
Vet Microbiol. 2005 Apr 25;107(1-2):49-62
15795077
J Virol. 1986 Apr;58(1):67-74
3005660
Res Vet Sci. 1979 Mar;26(2):202-8
233619
Clin Exp Immunol. 1993 Feb;91(2):272-6
7679060
Am J Hum Genet. 2003 Nov;73(5):1162-9
14574645
Nature. 1994 Mar 17;368(6468):215-21
8145819
Clin Vaccine Immunol. 2007 Oct;14(10):1274-8
17699832
AIDS. 1994 Oct;8(10):1421-7
7529507
Virology. 1999 Jun 5;258(2):355-64
10366572
Am J Hum Genet. 2001 Apr;68(4):978-89
11254454
Immunogenetics. 2005 Dec;57(11):855-63
16244883
J Am Vet Med Assoc. 1978 Dec 15;173(12):1575-7
218917
Neuropathol Appl Neurobiol. 1998 Jun;24(3):224-32
9717188
J Virol. 1992 May;66(5):2679-88
1348546
J Clin Virol. 2005 Jul;33(3):194-200
15911440
J Am Vet Med Assoc. 1990 Aug 15;197(4):475-9
2170310
Virology. 2007 May 25;362(1):226-34
17267002
Eur J Immunogenet. 2001 Aug;28(4):451-8
11532021
AIDS Res Hum Retroviruses. 2002 Jul 20;18(11):741-6
12167265
J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Feb 1;8(2):116-23
7834395
N Z Vet J. 2006 Aug;54(4):153-60
16915336
J Gen Virol. 1991 Aug;72 ( Pt 8):1905-11
1651984
J Virol Methods. 2000 Apr;86(1):13-20
10713371
JAMA. 1998 Nov 18;280(19):1690-1
9832001
J Infect Dis. 1992 Sep;166(3):531-41
1323619