Flow cytometric analysis of mitochondrial membrane potential using JC-1.
Changes in membrane potential have long been known to represent early activation events. This unit presents very recent developments in both fluorescent probes and functional applications and demonstrates the use of the JC-1 probe for measuring mitochondrial membrane potential by flow cytometry. A valuable component of this measurement system is the possibility of making quantitative measurements of changes in membrane potential.
Cossarizza
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A
University of Modena, Modena, Italy.
Salvioli
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S
D000818:Animals; D017209:Apoptosis; D001562:Benzimidazoles / Q000494:pharmacology*; D002232:Carbocyanines / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism
D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine