title: Gene expression of the human Torque Teno Virus isolate P/1C1.
abstract: Torque Teno Virus (TTV) has been assigned to the floating genus Anellovirus. TTV ssDNA genomes have a size of 3.6 to 3.8 kb and display up to 30% nucleotide diversity. The pathogenic potential of TTV is under investigation. To address a putative link of pathogenicity with the observed sequence variations, the transcription profile of P/1C1 (genogroup 1) isolated from a patient diseased with a non A-G hepatitis was analysed. Four mRNAs were identified, which encoded the seven proteins ORF1, ORF1/1, ORF1/2, ORF2, ORF2/2, ORF3 and ORF4. Expression of the ORF1 protein and its splice variant ORF1/1 in cell culture was detected by an ORF1-specific antiserum. Analysis of N-terminal tagged P/1C1-encoded proteins revealed that ORF1, ORF1/1 and ORF1/2 were localised in the nucleoli, ORF3 and ORF4 resided in the nucleoplasm, ORF2/2 appeared either in the nucleoli or the whole nucleus while ORF2 was the only protein seen in the cytoplasm.
authors.affiliation: Robert Koch-Institute, FG12 Division of Viral Infections, Robert Koch-Institute, Nordufer 20, 13353 Berlin, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Mueller
authors.forename: Alexander
authors.initials: A
authors.lastname: Maerz
authors.forename: Kai
authors.initials: K
authors.lastname: Doberstein
authors.forename: Tim
authors.initials: T
authors.lastname: Finsterbusch
authors.forename: Annette
authors.initials: A
authors.lastname: Mankertz
mesh_terms: D000595:Amino Acid Sequence; D004266:DNA Virus Infections / Q000235:genetics / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D006505:Hepatitis / Q000821:virology; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics; D020033:Protein Isoforms / Q000378:metabolism; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; D014764:Viral Proteins