title: Interactions between antitumor drugs and vault RNA.
abstract: It is supposed that ribonucleoprotein particle vault is involved in detoxification processes and thus is related to multidrug resistance. The vault is composed of three proteins and three vault RNAs, hvg-1, -2 and -3. The direct interactions between vault components and drugs were not reported. Recently, we revealed the interactions between vault RNAs and mitoxantrone. Here, we examined the interactions between hvg-2 and six antitumor drugs by a chemical shift perturbation method of NMR. It was found that in addition to mitoxantrone, hvg-2 can interact with two drugs basically in the same way using the same site. The difference in the affinity was also noticed among three drugs. Hvg-2 did not bind to the other three drugs. It is suggested that the common or closely related chemical structure of the positive three drugs is recognized by vault RNA.
authors.affiliation: Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Mashima
authors.forename: Michiko
authors.initials: M
authors.lastname: Kudo
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takada
authors.forename: Akimasa
authors.initials: A
authors.lastname: Matsugami
authors.forename: Subash C B
authors.initials: SC
authors.lastname: Gopinath
authors.forename: Penmetcha K R
authors.initials: PK
authors.lastname: Kumar
authors.forename: Masato
authors.initials: M
authors.lastname: Katahira
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D001483:Base Sequence; D008942:Mitoxantrone / Q000737:chemistry; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D012313:RNA / Q000737:chemistry*; D020394:Vault Ribonucleoprotein Particles / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D020394:Vault Ribonucleoprotein Particles; D012313:RNA; D008942:Mitoxantrone