Mouse gammadelta T cells are capable of expressing MHC class II molecules, and of functioning as antigen-presenting cells.
Although human and bovine gammadelta T cells were shown to express MHC class II antigen and function as APCs, attempts to determine if mouse gammabeta T cells have similar functions remained unsuccessful. We now show that gammadelta T cells derived from immunized mice also can be induced to express MHC class II and co-stimulatory molecules after activation in vitro, and are capable of antigen presentation. Using highly purified gammadelta T cells, we found that, unlike human gammadelta T cells, the expression of MHC class II molecules by mouse gammadelta T cells is limited to newly activated cells. Highest levels of MHC class II expression were seen on activated gammadelta T cells that had lost most surface-expressed gammadelta TCR while exhibiting increased levels of intracellular gammadelta TCR. In the absence of further stimulation, MHC class II expression gradually declined with the gammadelta T cells regaining their surface TCR. We also show that cytokine-activated gammadelta T cells can also express MHC class II antigen and exercise antigen-presenting activity.
Cheng
Lan
L
Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY, USA.
Cui
Yan
Y
Shao
Hui
H
Han
Gencheng
G
Zhu
Ling
L
Huang
Yafei
Y
O'Brien
Rebecca L
RL
Born
Willi K
WK
Kaplan
Henry J
HJ
Sun
Deming
D
D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002469:Cell Separation; D015536:Down-Regulation / Q000276:immunology; D005136:Eye Proteins / Q000276:immunology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D066298:In Vitro Techniques; D020381:Interleukin-17 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000378:metabolism; D000099214:Retinol-Binding Proteins, Interstitial
D005136:Eye Proteins; D000949:Histocompatibility Antigens Class II; D020381:Interleukin-17; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012177:Retinol-Binding Proteins; D051193:Toll-Like Receptors; D000099214:Retinol-Binding Proteins, Interstitial
J Immunol. 2004 Oct 15;173(8):5156-64
15470060
J Immunol. 2005 Apr 15;174(8):4678-87
15814692
Exp Eye Res. 2000 Aug;71(2):111-8
10930316
J Immunol. 2004 Jul 1;173(1):682-94
15210832
N Engl J Med. 1991 Mar 28;324(13):877-81
1705662
J Immunol. 1997 Dec 15;159(12):5787-94
9550374
Nat Med. 1999 Apr;5(4):405-11
10202929
J Exp Med. 1990 Mar 1;171(3):667-79
2137854
Eur J Immunol. 1999 Dec;29(12):4060-71
10602017
Int Immunol. 2003 Feb;15(2):261-8
12578856
Cell. 1989 May 19;57(4):667-74
2524273
Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6452-6
1830662
J Immunol. 2003 Nov 15;171(10):5507-13
14607957
Microbes Infect. 2005 Mar;7(3):529-36
15777712
J Immunol. 2006 Oct 1;177(7):4662-9
16982905
Science. 1989 May 12;244(4905):713-6
2524098
J Immunol. 2001 Jun 15;166(12):7579-87
11390514
J Immunol. 2005 Aug 1;175(3):1851-7
16034128
J Immunol. 1993 Dec 1;151(11):6546-58
8245484
J Immunol. 2006 Apr 15;176(8):5006-14
16585597
Immunol Rev. 2000 Feb;173:98-108
10719671
J Exp Med. 2000 Jul 17;192(2):219-26
10899908
Trends Immunol. 2006 Mar;27(3):112-8
16442347
Cell. 1997 Oct 31;91(3):295-8
9363937
Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):923-7
8430106
Adv Immunol. 1999;71:77-144
9917911
Ann Neurol. 1991 Dec;30(6):794-800
1838679
Annu Rev Immunol. 1995;13:251-76
7612223
Curr Opin Immunol. 2006 Feb;18(1):31-8
16337364
J Immunol. 1997 Feb 1;158(3):1217-21
9013962
Nat Med. 1998 Jul;4(7):764-5
9662359
J Exp Med. 1989 Nov 1;170(5):1569-81
2572671
J Exp Med. 1990 Oct 1;172(4):1225-31
2145388
J Immunol. 2000 Feb 15;164(4):2120-30
10657666
Annu Rev Immunol. 1993;11:637-85
8476575
Gastroenterology. 1991 Sep;101(3):670-8
1860632
Science. 2005 Jul 8;309(5732):264-8
15933162
Eur J Immunol. 1995 Oct;25(10):2877-81
7589086
J Leukoc Biol. 1998 Jun;63(6):707-14
9620663
Science. 2005 Aug 26;309(5739):1380-4
16123302
J Immunol. 2001 Apr 1;166(7):4327-33
11254685