title: PNA-peptide conjugates as intracellular gene control agents.
abstract: Serum-stabilized PNA-internalization peptides (Pip) conjugated to PNA complementary to the 705 aberrant beta-globin splice site are able to correct splicing and increase luciferase production in Hela pLuc705 cells with sub microM EC(50) in the absence of a transfection agent. Inhibition of microRNA-122 in liver cells is achieved by treatment with complementary PNA containing just a few attached Lys residues, again without need of a transfection agent.
authors.affiliation: Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK.
authors.forename: Gabriela D
authors.initials: GD
authors.lastname: Ivanova
authors.forename: Martin M
authors.initials: MM
authors.lastname: Fabani
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Arzumanov
authors.forename: Rachida
authors.initials: R
authors.lastname: Abes
authors.forename: Haifang
authors.initials: H
authors.lastname: Yin
authors.forename: Bernard
authors.initials: B
authors.lastname: Lebleu
authors.forename: Matthew
authors.initials: M
authors.lastname: Wood
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Gait
mesh_terms: D000818:Animals; D006367:HeLa Cells; D006801:Humans; D049409:Luciferases, Firefly / Q000096:biosynthesis; D051379:Mice; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020388:Muscular Dystrophy, Duchenne / Q000235:genetics; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012326:RNA Splicing; D055544:beta-Globins / Q000235:genetics
chemical_list: D035683:MicroRNAs; D020135:Peptide Nucleic Acids; D055544:beta-Globins; D049409:Luciferases, Firefly