A novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in Drosophila.
Translational read-through of the UGA stop codon is an evolutionarily conserved feature that most prominently represents the basis of selenoprotein biosynthesis. It requires a specific cis-acting stem loop control element, termed SECIS, which is located in the 3'-untranslated region of eukaryotic selenoprotein mRNAs. In a search for novel factors underlying the SECIS-directed UGA read-through process, we identified an evolutionary conserved GTPase-activating protein, termed GAPsec. We show that the activity of the Drosophila GAPsec (dGAPsec) is necessary to support SECIS-dependent UGA read-through activity in flies and the mouse homolog mGAPsec in mice tissue culture cells. However, selenoprotein biosynthesis is not impaired in flies that lack dGAPsec activity. The results indicate that GAPsec is part of a novel SECIS-dependent translational read-through system that does not involve selenocysteine incorporation.
Hirosawa-Takamori
Mitsuko
M
Max-Planck-Institut für biophysikalische Chemie, Abteilung Molekulare Entwicklungsbiologie, Am Fassberg 11, 37077 Göttingen, Germany.
Ossipov
Dmitri
D
Novoselov
Sergey V
SV
Turanov
Anton A
AA
Zhang
Yan
Y
Gladyshev
Vadim N
VN
Krol
Alain
A
Vorbrüggen
Gerd
G
Jäckle
Herbert
H
D000595:Amino Acid Sequence; D000818:Animals; D018388:Codon, Terminator / Q000378:metabolism*; D004330:Drosophila / Q000378:metabolism*; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D055029:Inverted Repeat Sequences / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D030781:Organisms, Genetically Modified; D017279:Selenocysteine / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D018388:Codon, Terminator; D029721:Drosophila Proteins; D020690:GTPase-Activating Proteins; D017279:Selenocysteine
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