Change in long-spacing collagen in Descemet's membrane of diabetic Goto-Kakizaki rats and its suppression by antidiabetic agents.
We examined changes in the ultrastructure and localization of major extracellular matrix components, including 5 types of collagen (type I, III, IV, VI, and VIII), laminin, fibronectin, and heparan sulfate proteoglycan in Descemet's membrane of the cornea of diabetic GK rats. In the cornea of diabetic GK rats, more long-spacing collagen fibrils were observed in Descemet's membrane than in the membrane of the nondiabetic Wistar rats. Both GK and Wistar rats showed an age-dependent increase in the density of the long-spacing collagen. Immunoelectron microscopy showed that type VIII collagen was localized in the internodal region of the long-spacing collagen, which was not labelled by any of the other antibodies used. The antidiabetic agents nateglinide and glibenclamide significantly suppressed the formation of the long-spacing collagen in the diabetic rats. Long-spacing collagen would thus be a useful indicator for studying diabetic changes in the cornea and the effect of antidiabetic agents.
Akimoto
Yoshihiro
Y
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan. yakimoto@kyorin-u.ac.jp
Sawada
Hajime
H
Ohara-Imaizumi
Mica
M
Nagamatsu
Shinya
S
Kawakami
Hayato
H
D000818:Animals; D003094:Collagen / Q000187:drug effects / Q000378:metabolism*; D003315:Cornea / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D003510:Cyclohexanes / Q000627:therapeutic use*; D003886:Descemet Membrane / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D003930:Diabetic Retinopathy / Q000473:pathology / Q000517:prevention & control; D005905:Glyburide / Q000627:therapeutic use*; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D000077715:Nateglinide; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012016:Reference Values
D003510:Cyclohexanes; D007004:Hypoglycemic Agents; D000077715:Nateglinide; D010649:Phenylalanine; D003094:Collagen; D005905:Glyburide
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