Cardiac mitochondrial membrane stability after deep hypothermia using a xenon clathrate cryostasis protocol - an electron microscopy study.
We investigated a new cryopreservation method using xenon, a clathrate-forming gas, under medium pressure (100psi). The objective of the study was to determine whether this cryostasis protocol could protect cardiac mitochondria at cryogenic temperatures (below 100 degrees Celsius).We analyzed transmission electron microscopy images to obtain information about changes in mitochondrial morphology induced by cryopreservation of the hearts. Our data showed absence of mitochondrial swelling, rupture of inner and outer membranes, and leakage of mitochondrial matrix into the cytoplasm after applying this cryostasis protocol. The electron microscopy results provided the first evidence that a cryostasis protocol using xenon as a clathrate-forming gas under pressure may have protective effects on intracellular membranes. This cryostasis technology may find applications in developing new approaches for long-term cryopreservation protocols.
Sheleg
Sergey
S
Innovative Biological Preservation Technologies LLC Scottsdale, AZ, USA. Sergey.Sheleg@asu.edu
Hixon
Hugh
H
Cohen
Bruce
B
Lowry
David
D
Nedzved
Mikhail
M
Annu Rev Physiol. 2001;63:359-90
11181960
Science. 1961 Jul 7;134(3471):15-21
13733483
Am J Physiol. 1984 Sep;247(3 Pt 1):C125-42
6383068
Proc Natl Acad Sci U S A. 1961 Sep 15;47:1515-24
13770890
J Biol Chem. 2003 Jul 11;278(28):25454-60
12702720
Zygote. 2005 Nov;13(4):283-93
16388696
Nat Biotechnol. 2000 Mar;18(3):296-9
10700144
Am J Physiol Cell Physiol. 2007 Jan;292(1):C157-63
16870828
Science. 2002 Apr 26;296(5568):655-6
11985355
Med Radiol (Mosk). 1973 Oct;18(10):26-8
4790636
Cryobiology. 2004 Apr;48(2):157-78
15094092
J Immunol. 1998 Oct 1;161(7):3753-9
9759901
J Cell Physiol. 2006 Feb;206(2):347-53
16110491
Cryobiology. 2003 Apr;46(2):197-202
12686211
Nature. 1997 Aug 21;388(6644):727-8
9285581
Nature. 1949 Oct 15;164(4172):666
18143360