Particle inflow gun-mediated transformation of multiple-shoot clumps in rhodes grass (Chloris gayana).
Rhodes grass (Chloris gayana) is one of the most important warm-season forage grasses. It is cultivated in tropical and subtropical parts of the world and is mostly used for grazing and hay production. We have established a particle-bombardment transformation protocol for rhodes grass using multiple-shoot clumps (MSCs) as the target tissue. A vector pAHC25 containing a herbicide-resistance gene (bar) together with the beta-glucuronidase (GUS) gene was used in transformation experiments. The most efficient recovery of bialaphos-resistant tissue was achieved when the bombarded MSCs were first cultured for 15 d on bialaphos-free medium before being subjected to selection pressure. The resistant tissues regenerated transgenic plants that displayed GUS gene expression. Under optimized conditions, 251 target pieces yielded 46 transgenic plants from 4 independent transgenic lines.
Gondo
Takahiro
T
Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan.
Matsumoto
Jun
J
Tsuruta
Shin-ichi
S
Yoshida
Midori
M
Kawakami
Akira
A
Terami
Fumihiro
F
Ebina
Masumi
M
Yamada
Toshihiko
T
Akashi
Ryo
R
D019470:Biolistics / Q000295:instrumentation* / Q000379:methods*; D018744:DNA, Plant / Q000378:metabolism; D047108:Embryonic Development; D005966:Glucuronidase / Q000378:metabolism; D009928:Organ Specificity; D018520:Plant Shoots / Q000378:metabolism*; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000196:embryology / Q000378:metabolism*; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
D018744:DNA, Plant; D005966:Glucuronidase