Laboratory maintenance of Brucella abortus.
This unit provides protocols for growth of Brucella abortus on solid or in liquid media and for long-term storage of laboratory stocks. Two issues affecting the culture and storage of isolates of this slow-growing bacterium are emphasized: contamination of cultures and outgrowth of attenuated variants lacking a complete lipopolysaccharide. Laboratories planning to work with B. abortus should be aware that Biosafety Level 3 facilities are required. Furthermore, this organism is classified in the U.S. as a Select Agent, which restricts its use to laboratories registered with the U.S. government's Select Agent programs.
Sun
Yao-Hui
YH
University of California at Davis, Davis, California, USA.
den Hartigh
Andreas B
AB
Tsolis
Renée M
RM
Ficht
Thomas A
TA
D001431:Bacteriological Techniques / Q000379:methods*; D002003:Brucella abortus / Q000166:cytology / Q000254:growth & development* / Q000302:isolation & purification; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
D003470:Culture Media