Changes in basic metabolic elements associated with the degeneration and ossification of ligamenta flava.
OBJECTIVE
To determine the association between levels of basic metabolic elements and degeneration and ossification of the ligamentum flavum (LF).


METHODS
Fourteen consecutive patients with degenerative lumbar stenosis, 11 with ossification of the thoracic ligamenta flava, and 11 control subjects.


METHODS
The basic elements of calcium (Ca), phosphorus (P), magnesium (Mg), zinc (Zn), copper (Cu), manganese (Mn), molybdenum (Mo), and fluoride (F) in the specimens were measured using atomic absorption spectrometry, the phosphomolybdic blue method, and a fluoride-selected electrode.


RESULTS
Ca content and the ratio of Ca/Mg in the LF specimens increased significantly in the sequence of control, degeneration, and ossification groups. Compared with values for the control group, the Zn, Mn, and Mo contents in the ossification and degeneration groups were significantly lower (P < 0.01); in contrast, Cu content was significantly higher (P < 0.01). As to F, its content in the specimens of the ossification group was much higher than those in the degeneration and control groups (P < 0.01); the F content in the ligamenta flava and sera from patients with fluorosis was also significantly higher than in those from patients without fluorosis (P < 0.01). Compared with the control group, there were no differences in the F content in serum from patients without fluorosis; however, the F content in ligamenta flava specimens from patients without fluorosis was significantly higher (P < 0.01).


CONCLUSIONS
There are trends in the contents of basic metabolic elements in the degeneration and ossification of ligamenta flava. These basic metabolic elements may play an important role in this process.
Wang
Zhe
Z
Institute of Orthopaedics, Xi-Jing Hospital, Fourth Military Medical University, Xi'an, Shannxi 710032, People's Republic of China.
Li
Xiang-Dong
XD
Li
Ming-Quan
MQ
Wang
Quan-Ping
QP
D000328:Adult; D016022:Case-Control Studies; D004602:Elements; D005260:Female; D005459:Fluorides / Q000378:metabolism; D006801:Humans; D017843:Ligamentum Flavum / Q000378:metabolism*; D008297:Male; D008345:Manganese / Q000378:metabolism; D008875:Middle Aged; D009999:Ossification, Heterotopic / Q000378:metabolism* / Q000473:pathology*; D010020:Osteonecrosis / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D012189:Retrospective Studies; D013054:Spectrophotometry, Atomic / Q000379:methods; D015032:Zinc / Q000378:metabolism
D004602:Elements; D010758:Phosphorus; D008345:Manganese; D015032:Zinc; D005459:Fluorides
J Spinal Disord Tech. 2004 Oct;17(5):395-400
15385879
Eur J Biochem. 1977 Nov 1;80(2):341-8
200424
Ann Neurol. 1979 Feb;5(2):193-5
426482
Spine (Phila Pa 1976). 1993 Nov;18(15):2267-70
8278844
Z Gerontol. 1994 Sep-Oct;27(5):328-9
7810200
Spine (Phila Pa 1976). 1992 Nov;17(11):1353-60
1462211
Clin Orthop Relat Res. 1985 Mar;(193):20-37
3971625
J Orthop Res. 1993 Jan;11(1):92-103
8380854
Neurol India. 2002 Sep;50(3):286-9
12391454
Bone. 1997 Jul;21(1):23-30
9213004
Neuroradiology. 1990;32(1):38-42
2110325
J Neurosurg. 1980 Feb;52(2):279-83
6243349
Neuroradiology. 1996 May;38(4):338-42
8738092
J Spinal Disord. 1991 Mar;4(1):110-3
1666964
J Neurosurg. 1987 Apr;66(4):511-8
3104552
Neurosurgery. 2005 Mar 1;56(3):E622
28184655
AJNR Am J Neuroradiol. 1987 Nov-Dec;8(6):991-4
3120555
Nihon Seikeigeka Gakkai Zasshi. 1986 May;60(5):523-8
3091742
Skeletal Radiol. 1993;22(3):211-3
8480212
J Neurosurg. 1982 Mar;56(3):448-50
6799620
Radiology. 1982 May;143(2):463-8
7071348