[Transcriptional repressive activity of mutated E2 protein of human papillomavirus 2 (HPV-2) variant].
Common warts are close associated with HPVs infection. In this study, we amplified and sequenced the LCR fragment and E2 gene of HPV-2 that infected the patient of extensive common wart with cutaneous horns, and we constructed the recombinant CAT-reporter plasmids pBLCAT-LCR containing HPV-2 prototype or variant LCR and mammalian expression plasmids pcDNA3. 1-E2 containing prototype or variant E2 ORF individually. The promoter activities of HPV-2 variant and the transcriptional repression activities of the mutated E2 protein were evaluated by transient transfection into HeLa cells. The results showed that there were several mutations in LCR and E2 gene of HPV-2 variant. Compared with the prototype, the viral early promoter activity of variant was significantly increased uder the control of LCR. Compared with the wild type E2 protein, the transcriptional repression activities of the mutated E2 protein was abolished partially. We speculate herein that increased promoter activities and decreased repression effect of the mutated E2 protein are linked, at least partially, with the clinical phenotypes of the uncommon huge common wart.
Lei
Yan-jun
YJ
School of Medicine, Xian Jiao-Tong University, Xian 710061, China. leiyj7268@sina.com
Gao
Chen
C
Jiang
Hui-ying
HY
Han
Jun
J
Chen
Jian-ming
JM
Shi
Qi
Q
Zhou
Wei
W
Yuan
Yu-kang
YK
Dong
Xiao-ping
XP
D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000502:physiology*; D027383:Papillomaviridae / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000502:physiology*; D014860:Warts / Q000821:virology*
D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins; C076036:oncogene protein E2, Human papillomavirus type 1