title: Bioactive glass modulation of intestinal epithelial cell restitution.
abstract: Repair of superficial injury to the gastrointestinal mucosa involves the process of restitution, the rapid migration of epithelial cells across damaged areas. The effect of 45S5 bioactive glass on epithelial restitution was assessed using a novel co-culture model incorporating wounded intestinal epithelial cell monolayers and sub-epithelial myofibroblasts to simulate in vivo conditions that occur during superficial mucosal ulceration. Epithelial wound healing was not increased by culture medium conditioned with bioactive glass, with 1% (w/v) bioactive glass inhibiting cell migration. Epithelial wounds co-cultured with myofibroblasts grown on surfaces coated with 0.1% (w/v) bioactive glass increased wound healing compared with co-cultures containing no bioactive glass. Myofibroblasts grown on surfaces coated with bioactive glass secreted significantly increased amounts of basic fibroblast growth factor but did not increase epithelial cell proliferation, indicating the wound healing observed was due to restitution. These data from a model of superficial mucosal injury suggest bioactive glass may function as a stimulant of paracrine mucosal signaling networks that promotes rapid epithelial repair.
authors.affiliation: Biomaterials and Tissue Engineering Group, Centre for Gastroenterology & Nutrition, Windeyer Institute, University College London, 46 Cleveland Street, London, W1T 4JF, UK.
authors.forename: Syed Raza
authors.initials: SR
authors.lastname: Moosvi
authors.forename: Richard M
authors.initials: RM
authors.lastname: Day
mesh_terms: D018938:Caco-2 Cells; D002516:Ceramics / Q000737:chemistry*; D018920:Coculture Techniques; D003470:Culture Media / Q000378:metabolism; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005898:Glass; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism*; D009092:Mucous Membrane / Q000378:metabolism; D014945:Wound Healing
chemical_list: D003470:Culture Media; D017077:Culture Media, Conditioned; C425330:bioactive glass 45S5; D016222:Fibroblast Growth Factor 2