Reactive oxygen species generation through NADH oxidation by pterin derivatives.
Pterin is an electron transfer compound in biological systems. Among the analogs, 6-formylpterin (6FP) has been demonstrated to have many marked physiological and pharmacological activities. In previous study, we have elucidated that 6FP derivatives in which the 3-position is modified possess reactive oxygen species (ROS), which are involved in the modulation of a variety of cell functions, generation activities through the oxidation of NADH to NAD(+) in the dark at neutral pH. In the present study, we have demonstrated that the ROS generation activity by 6FP derivative is enhanced in the presence of 3-methyl-1-phenyl-2-pyrazolin-5-one. In this reaction, 3-methyl-1-phenyl-2-pyrazolin-5-one is reacted with the formyl group on the 6-position of 6FP derivative to give the activated product. The present results would be helpful for designing pharmaceutical ROS generation system in vivo.
Nonogawa
Mitsuru
M
Institute of Advanced Energy, Kyoto University, Uji 611-0011, Japan.
Arai
Toshiyuki
T
Endo
Nobuyuki
N
Pack
Seung Pil
SP
Kodaki
Tsutomu
T
Makino
Keisuke
K
D000983:Antipyrine / Q000031:analogs & derivatives / Q000737:chemistry; D000077553:Edaravone; D009243:NAD / Q000737:chemistry*; D010084:Oxidation-Reduction; D011621:Pteridines / Q000737:chemistry*; D011622:Pterins / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000737:chemistry* / Q000378:metabolism
C504029:2-(N,N-dimethylaminomethyleneamino)-6-formyl-3-pivaloylpteridin-4-one; D011621:Pteridines; D011622:Pterins; D017382:Reactive Oxygen Species; D009243:NAD; D000077553:Edaravone; D000983:Antipyrine