Characterization of orchardgrass p23, a flowering plant Hsp90 cohort protein.
p23 is a heat shock protein 90 (Hsp90) co-chaperone and stabilizes the Hsp90 heterocomplex in mammals and yeast. In this study, we isolated a complementary DNA (cDNA) encoding p23 from orchardgrass (Dgp23) and characterized its functional roles under conditions of thermal stress. Dgp23 is a 911 bp cDNA with an open reading frame predicted to encode a 180 amino acid protein. Northern analysis showed that expression of Dgp23 transcripts was heat inducible. Dgp23 has a well-conserved p23 domain and interacted with an orchardgrass Hsp90 homolog in vivo, like mammalian and yeast p23 homologs. Recombinant Dgp23 is a small acidic protein with a molecular mass of approximately 27 kDa and pI 4.3. Dgp23 was also shown to function as a chaperone protein by suppression of malate dehydrogenase thermal aggregation. Differential scanning calorimetry thermograms indicated that Dgp23 is a heat-stable protein, capable of increasing the T (m) of lysozyme. Moreover, overexpression of Dgp23 in a yeast p23 homolog deletion strain, Deltasba1, increased cell viability. These results suggest that Dgp23 plays a role in thermal stress-tolerance and functions as a co-chaperone of Hsp90 and as a chaperone.
Cha
Joon-Yung
JY
Gyeongsang National University, Jinju, 660-701, South Korea.
Ermawati
Netty
N
Jung
Min Hee
MH
Su'udi
Mukhamad
M
Kim
Ki-Yong
KY
Kim
Jae-Yean
JY
Han
Chang-Deok
CD
Lee
Kon Ho
KH
Son
Daeyoung
D
D000595:Amino Acid Sequence; D000818:Animals; D031745:Dactylis / Q000235:genetics / Q000378:metabolism*; D005816:Genetic Complementation Test; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011489:Protein Denaturation; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques
D018841:HSP90 Heat-Shock Proteins; D018832:Molecular Chaperones; D010940:Plant Proteins
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