Sequential anaerobic-aerobic degradation of munitions waste.
A sequential anaerobic-aerobic biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) was studied. The results demonstrated that: (i) a complete degradation of RDX was achieved within 20 days using a consortium of bacteria from a wastewater activated sludge, (ii) RDX degradation did not occur under aerobic conditions alone, (iii) RDX-degrading bacterial strain that was isolated from the activated sludge completely degraded RDX within 2 days, and (iv) RDX- induced protein expressions were observed in the RDX-degrading bacterial strain. Based on fatty acid composition and a confirmation with a 16S rRNA analysis, the RDX-degrading bacterial strain was identified as a Bacillus pumilus-GC subgroup B.
Ibeanusi
Victor
V
Environmental Science and Studies Program, Spelman College, Atlanta, GA 30314, USA. vibeanus@spelman.edu
Jeilani
Yassin
Y
Houston
Samantha
S
Doss
Danielle
D
Coley
Bianca
B
D000332:Aerobiosis; D000693:Anaerobiosis; D001407:Bacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001673:Biodegradation, Environmental; D053834:Explosive Agents / Q000378:metabolism*; D006256:Hazardous Waste; D012722:Sewage; D014227:Triazines / Q000378:metabolism; D014865:Waste Disposal, Fluid
D001426:Bacterial Proteins; D053834:Explosive Agents; D006256:Hazardous Waste; D012722:Sewage; D014227:Triazines; C009160:cyclonite