title: Genes involved in the benzoate catabolic pathway in Acinetobacter calcoaceticus PHEA-2.
abstract: A putative benM gene encoding a LysR-type regulator located upstream from the benA gene was found in Acinetobacter calcoaceticus PHEA-2. Disruption of benM or benA destroyed the ability of PHEA-2 to utilize benzoate. The benM mutant was used to construct a genomic library for isolation of the complete gene cluster responsible for benzoate degradation. Sequence analysis showed that the cluster has three putative operons: benM, benABCDE, and benKP. Unlike many well-characterized benzoate-degrading bacteria, muconate is unable to induce in vivo transcription of the PHEA-2 ben cluster. Reverse transcriptase-polymerase chain reaction (RT-PCR) results showed that the benABCDE operon is activated by the BenM protein in the presence of benzoate. Moreover, a gel-retardation assay demonstrated that BenM binds to the promotor region of the benA gene. The activities of catechol 1,2-dioxygenase (C12O) and catechol 2,3-dioxygenase (C23O) showed that PHEA-2 converted benzoate to catechol for further degradation, possibly via an ortho-cleavage pathway.
authors.affiliation: College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Zhan
authors.forename: Haiying
authors.initials: H
authors.lastname: Yu
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Yan
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Shuying
authors.initials: S
authors.lastname: Li
authors.forename: Zixin
authors.initials: Z
authors.lastname: Peng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Ping
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Lin
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D001565:Benzoates / Q000378:metabolism*; D050548:Catechol 1,2-Dioxygenase / Q000378:metabolism; D050557:Catechol 2,3-Dioxygenase / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D017353:Gene Deletion; D020869:Gene Expression Profiling; D023061:Gene Order; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D001565:Benzoates; D004269:DNA, Bacterial; C005939:muconic acid; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D013011:Sorbic Acid
references.citation: Environ Microbiol. 2006 Mar;8(3):466-78
references.pmid: 16478453
references.citation: Appl Environ Microbiol. 2004 Jul;70(7):3814-20
references.pmid: 15240250
references.citation: J Mol Biol. 2007 Mar 30;367(3):616-29
references.pmid: 17291527
references.citation: J Bacteriol. 1987 Dec;169(12):5789-94
references.pmid: 2824447
references.citation: J Bacteriol. 1984 Oct;160(1):251-5
references.pmid: 6090417
references.citation: J Bacteriol. 2000 Nov;182(22):6339-46
references.pmid: 11053377
references.citation: Plasmid. 1985 Mar;13(2):149-53
references.pmid: 2987994
references.citation: Microb Cell Fact. 2006 Jan 05;5:1
references.pmid: 16396686
references.citation: Curr Microbiol. 2003 Apr;46(4):235-40
references.pmid: 12732969
references.citation: J Bacteriol. 2002 Nov;184(22):6301-15
references.pmid: 12399500
references.citation: J Bacteriol. 1998 May;180(9):2493-501
references.pmid: 9573203
references.citation: J Bacteriol. 1976 Jul;127(1):204-10
references.pmid: 931947
references.citation: J Bacteriol. 2001 Nov;183(22):6598-606
references.pmid: 11673430
references.citation: Appl Environ Microbiol. 2006 Mar;72(3):1749-58
references.pmid: 16517618
references.citation: Appl Environ Microbiol. 2006 Jan;72(1):932-6
references.pmid: 16391138
references.citation: Eur J Biochem. 1971 Jun 11;20(3):400-13
references.pmid: 4325686
references.citation: J Bacteriol. 1991 Sep;173(17):5385-95
references.pmid: 1885518
references.citation: Gene. 1994 Jul 22;145(1):69-73
references.pmid: 8045426