[Effect of sodium tungstate on glucose metabolism in adipocytes].
OBJECTIVE
To explore the effect of sodium tungstate on glucose metabolism in adipocytes and its mechanism.


METHODS
After 3T3-L1 preadipocytes were differentiated into adipocytes, these adipocytes were incubated with sodium tungstate (0, 150, 300, 500, and 700 micromol/L) for 48 h, and then glucose consumption of the adipocytes was detected by glucose-oxidase assay. Glucose transport was determined by the uptake of 2-deoxy-[3H]-D-glucose, and the expression of glucose transport-4 (GLUT-4) mRNA was identified by semi-quantitative RT-PCR.


RESULTS
Sodium tungstate (150 approximately 700 micromol/L) could significantly increase the glucose consumption and glucose transport with a concentration dependent-effect. Sodium tungstate could increase GLUT-4 mRNA expression.


CONCLUSIONS
Sodium tungstate can enhance the glucose metabolism of adipocytes by up-regulating the expression of GLUT-4 mRNA.
Yang
Yan
Y
Department of Endocrinology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Mo
Zhao-hui
ZH
Chen
Ke
K
He
Hong-hui
HH
Xiong
Jing
J
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000096:biosynthesis* / Q000235:genetics; D007004:Hypoglycemic Agents / Q000494:pharmacology; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017973:Tungsten Compounds / Q000494:pharmacology*; D015854:Up-Regulation
D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D012333:RNA, Messenger; D017973:Tungsten Compounds; C025399:sodium tungstate(VI); D005947:Glucose