title: Application of partially double-stranded DNA probes to high-throughput SNPs genotyping.
abstract: We have focused on DNA strand exchange reaction (SER) with partially double-stranded (PDS) probes as a novel platform of SNPs genotyping. In this report, we elaborated the method for reliable and high-throughput SNPs typing. Competitive reactions using a couple of PDS probes designed for wild and mutant sequences significantly increase resolution efficiency to SNP types that is hardly discriminated by a non-competitive reaction with a single probe. Integration of the PDS probes with DNA microarray technology enable us to increase throughput efficacy. Simple and quick typing of fifteen SNPs with reliability as high as a ligase-based assay was demonstrated.
authors.affiliation: Institute for Materials Chemistry and Engineering, Kyushu University, Motooka 744-CE11, Nishi-ku, Fukuoka, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Ishii
authors.forename: Kentaro
authors.initials: K
authors.lastname: Muraki
authors.forename: Naohiko
authors.initials: N
authors.lastname: Shimada
authors.forename: Arihiro
authors.initials: A
authors.lastname: Kano
authors.forename: Nao
authors.initials: N
authors.lastname: Nishida
authors.forename: Katsushi
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Atsushi
authors.initials: A
authors.lastname: Maruyama
mesh_terms: D020137:Base Pair Mismatch; D015342:DNA Probes / Q000737:chemistry*; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
chemical_list: D015342:DNA Probes