title: Methylenetetrahydrofolate reductase (MTHFR-677 and MTHFR-1298) genotypes and haplotypes and plasma homocysteine levels in patients with occlusive artery disease and deep venous thrombosis.
abstract: The aim was to investigate different genotypes and haplotypes of methylenetetrahydrofolate reductase (MTHFR-677, -1298) and plasma concentration of total homocysteine (tHcy) in Macedonian patients with occlusive artery disease (OAD) and deep venous thrombosis (DVT). Investigated groups consists of 80 healthy, 74 patients with OAD, and 63 patients with DVT. Plasma tHcy was measured with Microplate Enzyme Immunoassay. Identification of MTHFR genotypes and haplotypes was done with CVD StripAssay. The probability level (P-value) was evaluated by the Student's t-test. Plasma concentration of tHcy in CC and CT genotypes of MTHFR C677T was significantly increased in patients with OAD and in patients with DVT. Plasma concentration of tHcy in AC genotype of MTHFR A1298C was increased in patients with OAD and in patients with DVT. Plasma concentration of tHcy was significantly increased in AA genotype of patients with OAD, but not in patients with DVT. We found a significant increase of plasma tHcy in patients with OAD in comparison with healthy respondents for normal:heterozygote (CC:AC), heterozygote:normal (CT:AA), and heterozygote:heterozygote (CT:AC) haplotypes. Plasma concentration of tHcy in patients with DVT in comparison with healthy respondents was significantly increased for normal:normal (CC:AA), normal heterozygote (CC:AC), and heterozygote:heterozygote (CT:AC) haplotypes. We conclude that MTHFR C677T and MTHFR A1289C genotypes and haplotypes are connected with tHcy plasma levels in Macedonian patients with OAD and DVT.
authors.affiliation: Clinic for Cardiology, Faculty of Medicine, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia.
authors.forename: Igor
authors.initials: I
authors.lastname: Spiroski
authors.forename: Sashko
authors.initials: S
authors.lastname: Kedev
authors.forename: Slobodan
authors.initials: S
authors.lastname: Antov
authors.forename: Todor
authors.initials: T
authors.lastname: Arsov
authors.forename: Marija
authors.initials: M
authors.lastname: Krstevska
authors.forename: Sloboda
authors.initials: S
authors.lastname: Dzhekova-Stojkova
authors.forename: Gordana
authors.initials: G
authors.lastname: Bosilkova
authors.forename: Stojanka
authors.initials: S
authors.lastname: Kostovska
authors.forename: Dejan
authors.initials: D
authors.lastname: Trajkov
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Petlichkovski
authors.forename: Ana
authors.initials: A
authors.lastname: Strezova
authors.forename: Olivija
authors.initials: O
authors.lastname: Efinska-Mladenovska
authors.forename: Mirko
authors.initials: M
authors.lastname: Spiroski
mesh_terms: D000328:Adult; D000368:Aged; D001157:Arterial Occlusive Diseases / Q000097:blood / Q000201:enzymology* / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006579:Heterozygote; D006710:Homocysteine / Q000097:blood*; D006720:Homozygote; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D018843:Republic of North Macedonia; D020246:Venous Thrombosis / Q000097:blood / Q000201:enzymology* / Q000235:genetics*
chemical_list: D006710:Homocysteine; D042965:Methylenetetrahydrofolate Reductase (NADPH2)