Protective role of ascorbic acid isolated from Cissus quadrangularis on NSAID induced toxicity through immunomodulating response and growth factors expression.
The present study investigate the effect of ascorbic acid, the major bioactive component isolated from Cissus quadrangularis extract (CAA) on inflammatory cytokines and growth factors in non-steroidal anti-inflammatory drug (NSAID) induced gastric ulcer. Analysis of serum cytokine profile using enzymelinked immunosorbent assay (ELISA) showed a drastic increase in interleukin (IL)-1beta, IL-6, tumour necrosis factor-alpha (TNF)-alpha, interferon-gamma (IFN-gamma) and decrease in IL-10, Il-4 and prostaglandin E2 (PGE2) levels in NSAID (aspirin) treated rats. The reduction of growth factors such as transforming growth factor-alpha (TGF)-alpha and vascular endothelial cell growth factor (VEGF) by aspirin was determined by immunohistochemistry method. Administration of CAA produced significant protection against aspirin induced gastric toxicity by showing significant increase in PGE2, TGF-alpha, VEGF expression and accompanied by a significant inhibition of nitric oxide and regulating the levels of cytokines in rats. These findings suggest that CAA prevents gastric ulcer formation due to its immunomodulatory effect, antioxidant activity along with the ability to modulate PG synthesis and up-regulation of the growth factors.
Jainu
Mallika
M
Department of Biomedical Engineering, Siva Subramaniyam Nadar College of Engineering, SSN Nagar, Chennai - 603110, India. malsleo80@yahoo.co.in
Mohan
Kunju Vijai
KV
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity; D001205:Ascorbic Acid / Q000302:isolation & purification / Q000627:therapeutic use*; D001241:Aspirin / Q000633:toxicity; D032422:Cissus / Q000737:chemistry; D016207:Cytokines / Q000097:blood / Q000276:immunology*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000276:immunology; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood / Q000276:immunology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D009569:Nitric Oxide; D001205:Ascorbic Acid; D001241:Aspirin