[Transducin beta-TrCP expressed in developmental hair follicle of mice].
OBJECTIVE
To identify the expression of antagonist beta-TrCP protein in Sonic hedgehog signal transduction pathway and Wnt signal transduction pathway in hair follicle tissues.


METHODS
The heads of day 18 embryo, and one day and six-days-old postnatal mice were acquired and treated with 40 g/L paraformaldehyde fixation for 48 h and paraffin embedding. The expression of beta-TrCP proteins was examined using LsAB (labelled streptavidin-biotin) method.


RESULTS
beta-TrCP proteins were expressed in the cytoplasm of the hair stems of hair follicle, hair cuticle, cuticle of root sheath, Huxley's layer of internal root sheath cells, external root sheath and mesenchymal tissues, but not in connective tissue sheath and Henle's layer of internal root sheath.


CONCLUSIONS
TrCP express in the developmental hair follicle tissues, which implicates that beta-TrCP regulate the developmental hair follicle by mediating the signal transduction pathways.
Shen
Yong-dai
YD
State Key Laboratory of Oral Diseases, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
He
Yong-hong
YH
Yang
Lin
L
Tang
Wei
W
Liu
Lei
L
Tian
Wei-dong
WD
D000818:Animals; D000831:Animals, Newborn; D018859:Hair Follicle / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D015398:Signal Transduction; D013997:Time Factors; D044844:beta-Transducin Repeat-Containing Proteins / Q000096:biosynthesis* / Q000502:physiology*
D044844:beta-Transducin Repeat-Containing Proteins