Kinetic isotope effects in the oxidation of arachidonic acid by soybean lipoxygenase-1.
The reaction of soybean lipoxygenase-1 with linoleic acid has been extensively studied and displays very large kinetic isotope effects. In this work, substrate and solvent kinetic isotope effects as well as the viscosity dependence of the oxidation of arachidonic acid were investigated. The hydrogen atom abstraction step was rate-determining at all temperatures, but was partially masked by a viscosity-dependent step at ambient temperatures. The observed KIEs on k(cat) were large ( approximately 100 at 25 degrees C).
Jacquot
Cyril
C
Roger Adams Laboratory, Department of Chemistry, University of Illinois, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
Peng
Sheng
S
van der Donk
Wilfred A
WA
D016718:Arachidonic Acid / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007554:Isotopes; D007700:Kinetics; D019787:Linoleic Acid / Q000378:metabolism*; D008084:Lipoxygenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D013025:Glycine max / Q000201:enzymology*; D013696:Temperature
D007554:Isotopes; D016718:Arachidonic Acid; D019787:Linoleic Acid; C101084:lipoxygenase L-1; D008084:Lipoxygenase
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