Application of partially double-stranded DNA probes to high-throughput SNPs genotyping.
We have focused on DNA strand exchange reaction (SER) with partially double-stranded (PDS) probes as a novel platform of SNPs genotyping. In this report, we elaborated the method for reliable and high-throughput SNPs typing. Competitive reactions using a couple of PDS probes designed for wild and mutant sequences significantly increase resolution efficiency to SNP types that is hardly discriminated by a non-competitive reaction with a single probe. Integration of the PDS probes with DNA microarray technology enable us to increase throughput efficacy. Simple and quick typing of fifteen SNPs with reliability as high as a ligase-based assay was demonstrated.
Ishii
Tomoya
T
Institute for Materials Chemistry and Engineering, Kyushu University, Motooka 744-CE11, Nishi-ku, Fukuoka, Japan.
Muraki
Kentaro
K
Shimada
Naohiko
N
Kano
Arihiro
A
Nishida
Nao
N
Tokunaga
Katsushi
K
Maruyama
Atsushi
A
D020137:Base Pair Mismatch; D015342:DNA Probes / Q000737:chemistry*; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
D015342:DNA Probes