Transcriptional regulation of xyr1, encoding the main regulator of the xylanolytic and cellulolytic enzyme system in Hypocrea jecorina.
In Hypocrea jecorina, Xyr1 (xylanase regulator 1) is the main transcription activator of hydrolase-encoding genes, such as xyn1, xyn2, bxl1, cbh1, cbh2, egl1, and bgl1. Even though Xyr1 mediates the induction signal for all these genes derived from various inducing carbon sources and compounds, xyr1 transcription itself is not inducible by any of these substances. However, cultivation on glucose as the carbon source provokes carbon catabolite repression of xyr1 transcription mediated by Cre1. In addition, xyr1 transcription is repressed by the specific transcription factor Ace1. Moreover, Xyr1 is permanently available in the cell, and no de novo synthesis of this factor is needed for a first induction of xyn1 transcription. The constitutive expression of xyr1 leads to a significant elevation/deregulation of the xyn1, xyn2, and bxl1 transcription compared to what is seen for the parental strain. Overall, the corresponding xylanolytic enzyme activities are clearly elevated in a constitutively xyr1-expressing strain, emphasizing this factor as an auspicious target for genetically engineered strain improvement.
Mach-Aigner
Astrid R
AR
Gene Technology, Department of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Technische Universität Wien, Vienna, Austria.
Pucher
Marion E
ME
Steiger
Matthias G
MG
Bauer
Gudrun E
GE
Preis
Sonja J
SJ
Mach
Robert L
RL
D002244:Carbon / Q000378:metabolism; D044602:Cellulases / Q000096:biosynthesis; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D035901:Hypocrea / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
D005656:Fungal Proteins; D014157:Transcription Factors; D002244:Carbon; D044602:Cellulases; D043364:Endo-1,4-beta Xylanases; D005947:Glucose
Mol Microbiol. 1994 May;12(3):479-90
8065265
J Biol Chem. 1996 Oct 11;271(41):25624-9
8810338
Eukaryot Cell. 2006 Mar;5(3):447-56
16524900
Mol Microbiol. 1992 Nov;6(21):3225-35
1453960
FEBS Lett. 2007 Aug 21;581(21):3915-20
17662982
Mol Genet Genomics. 2003 Oct;270(1):46-55
12905071
Curr Genet. 2007 Nov;52(5-6):213-20
17924109
J Biol Chem. 2000 Feb 25;275(8):5817-25
10681571
Res Microbiol. 1999 May;150(4):281-5
10376490
Appl Environ Microbiol. 2003 Jan;69(1):56-65
12513977
Biochem J. 1997 Jan 15;321 ( Pt 2):375-81
9020869
Fungal Genet Biol. 2008 Apr;45(4):436-45
17920314
Gene. 1986;45(3):253-63
2948877
Fungal Genet Biol. 1999 Mar;26(2):131-40
10328983
Gene. 1987;56(1):117-24
2824287
Appl Environ Microbiol. 1998 Oct;64(10):3615-9
9758775
Mol Microbiol. 1996 Sep;21(6):1273-81
8898395
J Biol Chem. 2002 Apr 26;277(17):14688-94
11850429
Eur J Biochem. 1972 Oct;30(2):262-8
4576496
Trends Biotechnol. 1993 Oct;11(10):424-30
7764415
EMBO J. 1994 Sep 1;13(17):4022-7
8076597
Mol Microbiol. 2000 Apr;36(1):193-200
10760176
Appl Environ Microbiol. 2002 Sep;68(9):4546-53
12200312
Appl Microbiol Biotechnol. 2008 Feb;78(2):211-20
18197406
Fungal Genet Biol. 2008 Jun;45(6):984-93
18420433
Biochem Soc Trans. 1985 Apr;13(2):414-6
2991037
Eukaryot Cell. 2003 Feb;2(1):150-8
12582132
Appl Environ Microbiol. 1999 Oct;65(10):4340-5
10508057
Mol Microbiol. 1999 Apr;32(1):169-78
10216870
Science. 2006 Jan 27;311(5760):484-9
16439654
Curr Genet. 1992 May;21(6):471-7
1617736
Curr Genet. 1990 Jul;18(1):71-6
2245476
Eukaryot Cell. 2006 Dec;5(12):2128-37
17056741
Mol Gen Genet. 1996 Jun 24;251(4):451-60
8709949
Mol Microbiol. 1998 Jan;27(1):131-42
9466262
Appl Environ Microbiol. 1999 May;65(5):1858-63
10223970
Trends Biotechnol. 2006 Dec;24(12):549-56
17050014
J Biol Chem. 2001 Jun 29;276(26):24309-14
11304525
FEBS Lett. 1995 Nov 27;376(1-2):103-7
8521952
EMBO J. 1994 Jun 1;13(11):2493-501
8013449
Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7755-60
8755548
Science. 2007 Feb 9;315(5813):804-7
17289988