Reconstitution of iron oxidase from sulfur-grown Acidithiobacillus ferrooxidans.
The iron oxidation system from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells was reconstituted in vitro. Purified rusticyanin, cytochrome c, and aa(3)-type cytochrome oxidase were essential for reconstitution. The iron-oxidizing activity of the reconstituted system was 3.3-fold higher than that of the cell extract from which these components were purified.
Taha
Taher M
TM
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan. tahery1@yahoo.com
Kanao
Tadayoshi
T
Takeuchi
Fumiaki
F
Sugio
Tsuyoshi
T
D042763:Acidithiobacillus / Q000201:enzymology*; D001400:Azurin / Q000302:isolation & purification / Q000378:metabolism; D045304:Cytochromes c / Q000302:isolation & purification / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000302:isolation & purification / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D010088:Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism*
C012543:rusticyanin; D001400:Azurin; D045304:Cytochromes c; D007501:Iron; D010088:Oxidoreductases; D003576:Electron Transport Complex IV
J Biochem. 1992 Dec;112(6):816-21
1338330
Biosci Biotechnol Biochem. 2004 Dec;68(12):2519-28
15618623
Biochim Biophys Acta. 1989 Sep 28;976(2-3):129-34
2551385
Biosci Biotechnol Biochem. 1999 Sep;63(9):1541-7
10540740
FEMS Microbiol Lett. 2002 Apr 9;209(2):189-95
12007804
Eur J Biochem. 2001 Mar;268(6):1653-8
11248684
J Bacteriol. 1992 Jun;174(12):4189-92
1597434
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Microbiology (Reading). 2004 Jul;150(Pt 7):2113-2123
15256554
Nature. 1970 Aug 15;227(5259):680-5
5432063
Biochem J. 1978 Aug 15;174(2):497-502
708402
Biochim Biophys Acta. 2004 Jun 7;1656(2-3):114-26
15178473
Biosci Biotechnol Biochem. 2007 Nov;71(11):2663-9
17986795