Biotin sulfone as a new tool for synthetic oligosaccharide immobilization: application to multiple analysis profiling and surface plasmonic analysis of anti-Candida albicans antibody reactivity against alpha and beta (1-->2) oligomannosides.
As a part of our glycoantigen synthetic program for diagnosis and basic analysis of yeast-related pathogenic mechanisms, a library of 1-->2 oligomannosides suitable for immunoanalysis was prepared. The use of biotin sulfone, an oxidized form of biotin, offers a convenient solution for both oligosaccharide synthesis and immobilization on microspheres and surface plasmon resonance sensors. The application of this new strategy for the analysis of anti- Candida albicans antibody response through multiple-analyte profiling technology (Luminex) and with surface plasmonic analysis using biotin tagged synthetic oligosaccharides on avidin coated surfaces was validated using monoclonal antibodies.
Collot
Mayeul
M
Ecole Normale Supérieure, Département de Chimie, UMR CNRS 8642, Paris, France.
Sendid
Boualem
B
Fievez
Aurélie
A
Savaux
Camille
C
Standaert-Vitse
Annie
A
Tabouret
Marc
M
Drucbert
Anne Sophie
AS
Danzé
Pierre Marie
PM
Poulain
Daniel
D
Mallet
Jean-Maurice
JM
D000908:Antibodies, Fungal / Q000032:analysis* / Q000737:chemistry; D001360:Avidin / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D002176:Candida albicans / Q000276:immunology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007118:Immunoassay; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013450:Sulfones / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
D000908:Antibodies, Fungal; D009844:Oligosaccharides; D013450:Sulfones; C028569:oligomannoside; D001360:Avidin; C104349:biotin sulfone; D001710:Biotin