Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization.
Chemical inducers of dimerization (CIDs) are cell-permeable small molecules capable of dimerizing two protein targets. The most widely used CID, the natural product rapamycin and its relatives, is immunosuppressive due to interactions with endogenous targets and thus has limited utility in vivo. Here we report a new biocompatible CID, Tmp-SLF, which dimerizes E. coli DHFR and FKBP and has no endogenous mammalian targets that would lead to unwanted in vivo side effects. We employed Tmp-SLF to modulate gene expression in a yeast three-hybrid assay. Finally, we engineered the Golgi-resident glycosyltransferase FucT7 for tunable control by Tmp-SLF in mammalian cells.
Czlapinski
Jennifer L
JL
Department of Chemistry, University of California, Berkeley, California 94720, USA.
Schelle
Michael W
MW
Miller
Lawrence W
LW
Laughlin
Scott T
ST
Kohler
Jennifer J
JJ
Cornish
Virginia W
VW
Bertozzi
Carolyn R
CR
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006031:Glycosylation; D015394:Molecular Structure; D011485:Protein Binding; D055503:Protein Multimerization; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D001672:Biocompatible Materials
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