title: Aerosol vaccination against Newcastle disease: virus inhalation and retention during vaccination.
abstract: During aerosol vaccination of chickens with the LaSota strain of Newcastle disease virus, the quantities of inhaled and expired virus were determined by sampling the inhaled and expired air with a "capillary impinger", and virus retention in the respiratory tract was calculated. In serial experiments under smi-field conditions virus was nebulised in an amount of 4.4 +/- 0.4 log(10) EID50 per litre of air. The concentration of virus in the aerosol during 30 min following nebulisation was 2.8 +/- 0.4 logio EID50/1. The concentration of virus in the sampled air inhaled by the chickens as calculated per litre, was an average 2.4 +/- 0.49 log(10) EID50/1 and in the expired air 1 to 100 EID50/1. Comparison of the results shows that virus retention is such as to permit lodgement of a sufficient number of particles to produce what has been shown elsewhere to be an effective immunisation.
authors.affiliation: Poultry Department, Central Veterinary Institute, Doorn, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Yadin