A short C-rich PNA fragment capable to form novel G-quadruplex-PNA complexes.
In this work we investigated the interaction between the short ac(4)a C-rich peptide nucleic acid (PNA) probe and two intramolecular G-quadruplex targets having the same G-tetrad core, but different folding topologies. The T(G(4)T)(3)G(4)T and the recently reported tetra-end-linked-(TG(4)T)(4) G-rich oligonucleotides (GROs) were chosen and synthesized for this study. UV, CD, and MS experiments revealed the formation of novel 1:1 G-quadruplex-PNA complexes besides the expected DNA-PNA heteroduplexes.
Amato
Jussara
J
Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Via D. Montesano 49, I-80131 Napoli, Italy.
Gabelica
Valérie
V
Borbone
Nicola
N
Rosu
Frédéric
F
De Pauw
Edwin
E
Oliviero
Giorgia
G
Piccialli
Gennaro
G
Mayol
Luciano
L
D002942:Circular Dichroism; D003596:Cytosine / Q000737:chemistry; D054856:G-Quadruplexes; D009841:Oligonucleotides / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D003596:Cytosine