title: Total light approach of time-domain fluorescence diffuse optical tomography.
abstract: In this study, time-domain fluorescence diffuse optical tomography in biological tissue is numerically investigated using a total light approach. Total light is a summation of excitation light and zero-lifetime emission light divided by quantum yield. The zero-lifetime emission light is an emitted fluorescence light calculated by assuming that the fluorescence lifetime is zero. The zero-lifetime emission light is calculated by deconvolving the actually measured emission light with a lifetime function, an exponential function for fluorescence decay. The object for numerical simulation is a 2-D 10 mm-radius circle with the optical properties simulating biological tissues for near infrared light, and contains regions with fluorophore. The inverse problem of fluorescence diffuse optical tomography is solved using time-resolved simulated measurement data of the excitation and total lights for reconstructing the bsorption coefficient and fluorophore concentration simultaneously. The mean time of flight is used as the featured data-type extracted from the time-resolved data. The reconstructed images of fluorophore concentration show good quantitativeness and spatial reproducibility. By use of the total light approach, computation is performed much faster than the conventional ones.
authors.affiliation: Department of Mechanical Engineering and Intelligent Systems, University of Electro-Communications, Chofu, Tokyo, Japan. marjono@ymdlab.mce.uec.ac.jp
authors.forename: Andhi
authors.initials: A
authors.lastname: Marjono
authors.forename: Akira
authors.initials: A
authors.lastname: Yano
authors.forename: Shinpei
authors.initials: S
authors.lastname: Okawa
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamada
mesh_terms: D000465:Algorithms; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods; D008029:Lighting / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D041622:Tomography, Optical / Q000295:instrumentation*