Gating-ML: XML-based gating descriptions in flow cytometry.
The lack of software interoperability with respect to gating due to lack of a standardized mechanism for data exchange has traditionally been a bottleneck, preventing reproducibility of flow cytometry (FCM) data analysis and the usage of multiple analytical tools. To facilitate interoperability among FCM data analysis tools, members of the International Society for the Advancement of Cytometry (ISAC) Data Standards Task Force (DSTF) have developed an XML-based mechanism to formally describe gates (Gating-ML). Gating-ML, an open specification for encoding gating, data transformations and compensation, has been adopted by the ISAC DSTF as a Candidate Recommendation. Gating-ML can facilitate exchange of gating descriptions the same way that FCS facilitated for exchange of raw FCM data. Its adoption will open new collaborative opportunities as well as possibilities for advanced analyses and methods development. The ISAC DSTF is satisfied that the standard addresses the requirements for a gating exchange standard.
Spidlen
Josef
J
Terry Fox Laboratory, BC Cancer Agency, Vancouver, British Columbia, Canada.
Leif
Robert C
RC
Moore
Wayne
W
Roederer
Mario
M
Brinkman
Ryan R
RR
D002469:Cell Separation / Q000592:standards*; D005434:Flow Cytometry / Q000592:standards*; D016247:Information Storage and Retrieval / Q000592:standards*; D007256:Information Systems / Q000592:standards*; D012984:Software / Q000592:standards*
Cytometry. 2001 Jul 1;44(3):195-209
11429770
Cytometry A. 2008 Oct;73(10):926-30
18752282
Cytometry. 1990;11(3):323-32
2340769
Nature. 2002 Oct 17;419(6908):751, 753, 755 passim
12384707
Cytometry A. 2008 Apr;73(4):321-32
18307272
Cytometry A. 2006 Jun;69(6):541-51
16604519
OMICS. 2006 Summer;10(2):209-14
16901228
Cytometry. 1997 Jun 1;28(2):118-22
9181300
J Immunol Methods. 2004 Sep;292(1-2):59-71
15350512
Cytometry A. 2005 Mar;64(1):34-42
15700280
Cytometry A. 2007 Sep;71(9):646-7
17680704
Clin Chem. 2002 Oct;48(10):1819-27
12324512
BMC Immunol. 2005 Jun 24;6:13
15978127