Susceptibility of peripheral lymphocytes of brain tumour patients to in vitro radiation-induced DNA damage, a preliminary study.
OBJECTIVE
The present investigation aimed to study the susceptibility of lymphocytes collected from brain tumour patients to radiation-induced DNA damage under in vitro conditions.


METHODS
The peripheral lymphocytes collected from brain tumour patients were exposed to 2-Gy gamma radiation. Susceptibility of lymphocytes to radiation-induced DNA damage and their repair ability was assessed by alkaline comet assay.


RESULTS
Lymphocytes of patients with benign and malignant tumour had a significantly higher (p < 0.001) baseline DNA damage compared to lymphocytes from normal subjects. A significant increase (p < 0.001) in DNA damage was observed immediately after irradiation of lymphocytes from healthy subjects and brain tumour patients. However, at 1 h after irradiation the level of DNA damage dropped significantly (p < 0.001) compared to that of immediately after irradiation of respective groups.


CONCLUSIONS
In conclusion, the lymphocytes of brain tumour patients possess a higher level of basal DNA damage and exhibit a higher susceptibility to a clastogenic agent like radiation.
Kalthur
Guruprasad
G
Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Medical College and Hospital, Manipal, Karnataka, India.
Kumar
Prem
P
Devi
Uma
U
Ali
Sabir
S
Upadhya
Ramya
R
Pillai
Sailaja
S
Rao
Anjali
A
D000328:Adult; D001932:Brain Neoplasms / Q000097:blood* / Q000532:radiotherapy; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male
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