Advanced preparative techniques to establish probes for molecular cytogenetics.
The methods covered in this unit include flow cytometry of metaphase chromosomes, chromosome dissection, and the DOP-PCR amplification methods for reverse chromosome painting. Successful application in these areas requires care and attention to methodological details, and this unit is particularly comprehensive.
Stap
J
J
University of Amsterdam, Amsterdam, The Netherlands.
Aten
J A
JA
Lillington
D
D
Shelling
A
A
Young
B D
BD
D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure; D003582:Cytogenetics / Q000379:methods*; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008677:Metaphase; D015345:Oligonucleotide Probes / Q000032:analysis*
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes