title: Search for informative polymorphisms in candidate genes: clock genes and circadian behaviour in blue tits.
abstract: The identification of functional polymorphisms in genes that underlie behavioural trait variation is a challenging but intriguing task in evolutionary biology. Given the wealth of genomic data and the increasing number of genotype-phenotype association studies in model organisms, one can ask whether and how this information can be used for non-model organisms. Here we describe two strategies to search for likely functional polymorphisms in candidate genes in a bird species that has been intensively studied by behavioural and population ecologists, the blue tit Cyanistes caeruleus. In the first approach we searched for repeating elements in coding regions of the genome using information about repeats in Gallus gallus genes. The rationale is that tandem-repeat elements have a high potential to be polymorphic and functional. The second strategy aimed to replicate reported genotype-phenotype association studies by extrapolating results from model organisms to our study species. Both strategies showed high success rates with respect to finding homologous gene regions and potentially informative genetic variants in the genes AANAT, ADCYAP1, CKIepsilon, CLOCK, CREB1, NPAS2 and PERIOD2.
authors.affiliation: Max Planck Institute for Ornithology (Seewiesen), Postfach 1564, 82305 Starnberg, Seewiesen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Steinmeyer
authors.forename: J C
authors.initials: JC
authors.lastname: Mueller
authors.forename: B
authors.initials: B
authors.lastname: Kempenaers
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D056926:CLOCK Proteins; D002940:Circadian Rhythm / Q000235:genetics*; D016678:Genome; D005838:Genotype; D006801:Humans; D046109:Passeriformes / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D030161:Avian Proteins; D015534:Trans-Activators; D056926:CLOCK Proteins; C538943:CLOCK protein, human
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