title: Association of mast cells with lung function in chronic obstructive pulmonary disease.
abstract: BACKGROUND
  In asthma, higher chymase positive mast cell (MC-C) numbers are associated with less airway obstruction. In COPD, the distribution of MC-C and tryptase positive mast cells (MC-T) in central and peripheral airways, and their relation with lung function, is unknown. We compared MC-T and MC-C distributions in COPD and controls without airflow limitation, and determined their relation with lung function.
  
  
  METHODS
  Lung tissue sections from 19 COPD patients (median [interquartile range] FEV1% predicted 56 [23-75]) and 10 controls were stained for tryptase and chymase. Numbers of MC-T and MC-C were determined in different regions of central and peripheral airways and percentage of degranulation was determined.
  
  
  RESULTS
  COPD patients had lower MC-T numbers in the subepithelial area of central airways than controls. In COPD, MC-T numbers in the airway wall and more specifically in the epithelium and subepithelial area of peripheral airways correlated positively with FEV1/VC (Spearman's rho (rs) 0.47, p = 0.05 and rs 0.48, p = 0.05, respectively); MC-C numbers in airway smooth muscle of peripheral airways correlated positively with FEV1% predicted (rs 0.57, p = 0.02). Both in COPD patients and controls the percentage of degranulated MC-T and MC-C mast cells was higher in peripheral than in central airways (all p < 0.05), but this was not different between the groups.
  
  
  CONCLUSIONS
  More MC-T and MC-C in peripheral airways correlate with better lung function in COPD patients. It is yet to determine whether this reflects a protective association of mast cells with COPD pathogenesis, or that other explanations are to be considered.
authors.affiliation: Department of Pulmonology, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands. margotgosman@hotmail.com
authors.forename: Margot M E
authors.initials: MM
authors.lastname: Gosman
authors.forename: Dirkje S
authors.initials: DS
authors.lastname: Postma
authors.forename: Judith M
authors.initials: JM
authors.lastname: Vonk
authors.forename: Bea
authors.initials: B
authors.lastname: Rutgers
authors.forename: Monique
authors.initials: M
authors.lastname: Lodewijk
authors.forename: Mieke
authors.initials: M
authors.lastname: Smith
authors.forename: Marjan A
authors.initials: MA
authors.lastname: Luinge
authors.forename: Nick H T
authors.initials: NH
authors.lastname: Ten Hacken
authors.forename: Wim
authors.initials: W
authors.lastname: Timens
mesh_terms: D000368:Aged; D001980:Bronchi / Q000166:cytology / Q000276:immunology; D016022:Case-Control Studies; D053818:Chymases / Q000378:metabolism*; D004847:Epithelial Cells / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000201:enzymology*; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis* / Q000276:immunology*; D012129:Respiratory Function Tests; D053802:Tryptases / Q000378:metabolism
chemical_list: D053818:Chymases; D053802:Tryptases
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