Characterization of Bacillus halodurans alpha-galactosidase Mel4A encoded by the mel4A gene (BH2228).
A family-4 alpha-galactosidase Mel4A of Bacillus halodurans was expressed in Escherichia coli and characterized. Recombinant enzyme rMel4A depended on NAD+, some divalent cations such as Mn2+, and reducing reagents such as dithiothreitol. rMel4A was active on small saccharides such as raffinose but not on highly polymerized galactomannan. Immunological analysis indicated that raffinose induced the production of Mel4A in B. halodurans.
Anggraeni
Andian Ari
AA
Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University, Tsu, Japan.
Sakka
Makiko
M
Kimura
Tetsuya
T
Ratanakhaokchai
Khanok
K
Kitaoka
Motomitsu
M
Sakka
Kazuo
K
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008345:Manganese / Q000378:metabolism; D008553:Melibiose / Q000235:genetics*; D009243:NAD / Q000378:metabolism / Q000494:pharmacology; D011887:Raffinose / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D000519:alpha-Galactosidase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
D002413:Cations, Divalent; D011994:Recombinant Proteins; D009243:NAD; D008345:Manganese; D008553:Melibiose; D000519:alpha-Galactosidase; D011887:Raffinose; D004229:Dithiothreitol