title: Diversity of yeasts from bovine mastitis in Southern Brazil.
abstract: Mastitis is one of the most serious problems in the dairy cattle farms. The great majority of the cases are caused by bacteria, but lately there have been an increasing number of reports about cases of mycotic etiology. The objective of this work was to characterize the yeasts and yeast-like fungi associated with milk of cows with mastitis. Milk samples (n = 248) from a dairy belt situated around the region of Passo Fundo, hinterland of the state of Rio Grande do Sul, Southern Brazil, were analyzed. Aliquots of 0.1 ml of milk were inoculated on yeast extract-malta agar with chloramphenicol. After a period of incubation of 3-5 days at 22-25 degrees C, the counting of the morphologically distinct colonies was performed, as well as the isolation and identification through phenotypical and physiological criteria. It was possible to isolate 68 yeast species from 43 (17.3%) of the samples. The most frequent genera were Candida (37.9%), Pichia (19.1%), Cryptococcus (10.3%) and Rhodotorula (10.3%).
authors.affiliation: Setor de Micologia Veterinária, Departamento de Patologia Clínica Veterinária, Faculdade de Medicina Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre RS, Brazil. andreiaspanamberg@yahoo.com.br
authors.forename: Andréia
authors.initials: A
authors.lastname: Spanamberg
authors.forename: Elsio Augusto
authors.initials: EA
authors.lastname: Wünder
authors.forename: Daniela Isabel
authors.initials: DI
authors.lastname: Brayer Pereira
authors.forename: Juliana
authors.initials: J
authors.lastname: Argenta
authors.forename: Edna Maria
authors.initials: EM
authors.lastname: Cavallini Sanches
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valente
authors.forename: Laerte
authors.initials: L
authors.lastname: Ferreiro
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D008414:Mastitis, Bovine / Q000382:microbiology*; D015003:Yeasts / Q000302:isolation & purification*