MicroRNAs as biomarkers in tomato leaf curl virus (ToLCV) disease.
MicroRNAs are approximately 21- 25 nt long RNA species that are critical regulators of transcriptome across the eukaryotes. Growing number of evidences clearly supports their involvement in plant leaf development. ToLCV infection severely affects the morphology of mature tomato leaves. To investigate the mechanism underlying the virus- host interaction, we focussed our studies on expression of microRNAs and the irrespective targets under normal and ToLCV infection. We have cloned Myb33, ARF4 homolog, Argonaute1, Apetala2, SBP transcription factor and RBOH from tomato and checked their expression by RT-PCR. Our work suggests that miR159 is upregulated while miR164 and miR171 are downregulated under viral infection. Our studies shed light on the impact of ToLCV infection on host transcriptome.
Naqvi
Afsar Raza
AR
Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
Choudhury
Nirupam Roy
NR
Haq
Qazi Mohd Rizwanul
QM
Mukherjee
Sunil Kumar
SK
D053839:Begomovirus; D015415:Biomarkers / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D035683:MicroRNAs / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*
D015415:Biomarkers; D035683:MicroRNAs