title: Rab13 regulates membrane trafficking between TGN and recycling endosomes in polarized epithelial cells.
abstract: To maintain polarity, epithelial cells continuously sort transmembrane proteins to the apical or basolateral membrane domains during biosynthetic delivery or after internalization. During biosynthetic delivery, some cargo proteins move from the trans-Golgi network (TGN) into recycling endosomes (RE) before being delivered to the plasma membrane. However, proteins that regulate this transport step remained elusive. In this study, we show that Rab13 partially colocalizes with TGN38 at the TGN and transferrin receptors in RE. Knockdown of Rab13 with short hairpin RNA in human bronchial epithelial cells or overexpression of dominant-active or dominant-negative alleles of Rab13 in Madin-Darby canine kidney cells disrupts TGN38/46 localization at the TGN. Moreover, overexpression of Rab13 mutant alleles inhibits surface arrival of proteins that move through RE during biosynthetic delivery (vesicular stomatitis virus glycoprotein [VSVG], A-VSVG, and LDLR-CT27). Importantly, proteins using a direct route from the TGN to the plasma membrane are not affected. Thus, Rab13 appears to regulate membrane trafficking between TGN and RE.
authors.affiliation: Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Rita L
authors.initials: RL
authors.lastname: Nokes
authors.forename: Ian C
authors.initials: IC
authors.lastname: Fields
authors.forename: Ruth N
authors.initials: RN
authors.lastname: Collins
authors.forename: Heike
authors.initials: H
authors.lastname: Fölsch
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000378:metabolism; D016764:Cell Polarity; D002478:Cells, Cultured; D004285:Dogs; D011992:Endosomes / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation; D021381:Protein Transport / Q000502:physiology; D011990:Receptors, Transferrin / Q000032:analysis / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D001324:Autoantigens; C028391:G protein, vesicular stomatitis virus; C097741:Golgin subfamily A member 2; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011990:Receptors, Transferrin; C072237:TGOLN2 protein, human; D014759:Viral Envelope Proteins; C085485:RAB13 protein, human; D020691:rab GTP-Binding Proteins
references.citation: Curr Opin Cell Biol. 2008 Apr;20(2):208-13
references.pmid: 18282697
references.citation: Trends Cell Biol. 2002 Jun;12(6):247-9
references.pmid: 12074877
references.citation: J Cell Biol. 2004 Nov 8;167(3):531-43
references.pmid: 15534004
references.citation: J Cell Biol. 2003 Oct 27;163(2):339-50
references.pmid: 14581456
references.citation: Trends Cell Biol. 2005 Apr;15(4):222-8
references.pmid: 15817379
references.citation: Mol Biol Cell. 2007 Jul;18(7):2687-97
references.pmid: 17494872
references.citation: Mol Biol Cell. 2006 Mar;17(3):1286-97
references.pmid: 16394106
references.citation: J Cell Biol. 2007 Jul 30;178(3):363-9
references.pmid: 17646400
references.citation: Traffic. 2007 Jan;8(1):47-60
references.pmid: 17132146
references.citation: J Cell Biol. 1998 Aug 24;142(4):923-36
references.pmid: 9722606
references.citation: Nature. 2003 Apr 17;422(6933):766-74
references.pmid: 12700771
references.citation: Cell. 2007 Jun 15;129(6):1201-13
references.pmid: 17574030
references.citation: J Biol Chem. 2007 Jan 19;282(3):2029-37
references.pmid: 17074766
references.citation: J Cell Biol. 2004 Apr 26;165(2):175-80
references.pmid: 15096524
references.citation: Ann N Y Acad Sci. 2004 Apr;1014:164-9
references.pmid: 15153431
references.citation: Mol Cell. 2003 Nov;12(5):1064-6
references.pmid: 14636566
references.citation: Mol Cell Biol. 2006 Oct;26(19):7299-317
references.pmid: 16980630
references.citation: Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1564-9
references.pmid: 17244703
references.citation: Methods Enzymol. 2005;403:19-28
references.pmid: 16473574
references.citation: J Cell Biol. 2003 Oct 27;163(2):351-62
references.pmid: 14581457
references.citation: Biochem Biophys Res Commun. 2003 Aug 22;308(2):270-5
references.pmid: 12901864
references.citation: J Cell Biol. 2007 May 7;177(3):477-88
references.pmid: 17485489
references.citation: Cell. 1999 Oct 15;99(2):189-98
references.pmid: 10535737
references.citation: J Biol Chem. 2005 Jan 21;280(3):2220-8
references.pmid: 15528189
references.citation: Mol Biol Cell. 2002 Jun;13(6):1819-31
references.pmid: 12058051
references.citation: J Biol Chem. 1998 Oct 9;273(41):26862-9
references.pmid: 9756932
references.citation: J Cell Sci. 1999 Jan;112 ( Pt 1):21-33
references.pmid: 9841901
references.citation: Nat Cell Biol. 2002 Feb;4(2):154-9
references.pmid: 11802162
references.citation: Mol Biol Cell. 2008 Mar;19(3):971-83
references.pmid: 18094055
references.citation: Mol Biol Cell. 2007 Dec;18(12):4872-84
references.pmid: 17881725
references.citation: J Mol Biol. 2001 Nov 2;313(4):889-901
references.pmid: 11697911
references.citation: Nat Cell Biol. 2003 Apr;5(4):287-93
references.pmid: 12669082
references.citation: Mol Biol Cell. 2006 Jul;17(7):3156-75
references.pmid: 16641372
references.citation: J Cell Sci. 2004 Dec 1;117(Pt 25):5955-64
references.pmid: 15564373