title: Enhanced amplification of polymerase chain reaction by addition of cosolutes derived from a cellular compatible solute.
abstract: We designed and synthesized artificial compatible solutes which can not only decrease the melting temperature of DNA duplexes dependent of their chemical structures but also improve the amplification of highly stable genome DNA sequence.
authors.affiliation: FIBER, Konan University, Higashinada-ku, Kobe, 658-8501, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Koumoto
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ochiai
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
mesh_terms: D001622:Betaine / Q000031:analogs & derivatives*; D004247:DNA / Q000737:chemistry; D006801:Humans; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction
chemical_list: D001622:Betaine; D004247:DNA