title: Temperature-gradient dependent detection of target DNA oligomers using DNA-immobilized open tubular capillary column.
abstract: A DNA-immobilized open tubular capillary column (DNA-immobilized OTC) system was developed for analysis and separation of target DNA oligomers. By combining the DNA-immobilized OTC column with a nano/micro-flow pump and a high-sensitivity UV detector, we succeeded in small-scale selective detection of target DNA oligomers (> 0.02 pmol). We designed a temperature-gradient strategy for the efficient separation of target DNA.
authors.affiliation: Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
authors.forename: Nagendra Kumar
authors.initials: NK
authors.lastname: Kamisetty
authors.forename: Seung Pil
authors.initials: SP
authors.lastname: Pack
authors.forename: Kamakshaiah Charyulu
authors.initials: KC
authors.lastname: Devarayapalli
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nonogawa
authors.forename: Tsutomo
authors.initials: T
authors.lastname: Kodaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Makino
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D015342:DNA Probes; D009838:Oligodeoxyribonucleotides