title: Association of CYP1A1 and glutathione S-transferase polymorphisms with male factor infertility.
abstract: OBJECTIVE
  To examine whether a relationship exists between genetic polymorphisms of glutathione S-transferase (GST) M1 and T1, CYP1A1(*)2C, and male factor infertility.
  
  
  METHODS
  Genetic polymorphism analysis, case-control study.
  
  
  METHODS
  University research laboratory and andrology clinic.
  
  
  METHODS
  One hundred ten men with infertility and 105 healthy fertile men were recruited for the study.
  
  
  METHODS
  Physical examination of the genitalia of patients, scrotal colored Doppler ultrasound examination, and blood sampling were performed for DNA extraction and genotyping.
  
  
  METHODS
  CYP1A1(*)2C, GSTM1, and GSTT1 polymorphism genotypes were determined by polymerase chain reaction and restriction fragment length polymorphism methods. Seminal parameters were analyzed.
  
  
  RESULTS
  There were significant differences between infertility and GSTM1, CYP1A1(*)2C genotypes by univariate analyses. A subject carrying CYP1A1 Val/Val or CYP1A1 Ile/Val in association with GSTM null genotype has 6.90 times more risk to be infertile than a subject carrying CYP1A1 Ile/Ile in association with GSTM1 wild-type genotype (odds ratio: 6.90, 95% confidence interval: 2.29-19.3). No correlation was found between the seminal parameters and the genetic variability.
  
  
  CONCLUSIONS
  Our results suggest that genetic polymorphisms of xenobiotic-metabolizing enzymes could play an important role in infertility.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Ankara University, Ankara, Turkey.
authors.forename: Sena Erdogan
authors.initials: SE
authors.lastname: Aydos
authors.forename: Mehmet
authors.initials: M
authors.lastname: Taspinar
authors.forename: Asuman
authors.initials: A
authors.lastname: Sunguroglu
authors.forename: Kaan
authors.initials: K
authors.lastname: Aydos
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000737:chemistry*; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D015994:Incidence; D007248:Infertility, Male / Q000453:epidemiology* / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018570:Risk Assessment; D012307:Risk Factors; D014421:Turkey / Q000453:epidemiology
chemical_list: D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase