Gene transfer and living release of transforming growth factor-beta3 for cartilage tissue engineering applications.
Cartilage restoration continues to present a tremendous clinical challenge due to its nonvascular nature. Many studies have demonstrated that chondrogenesis of progenitor cells can be achieved in vitro by manual dose of growth factors; however, it remains a vital difficulty in feeding growth factors to implanted therapeutic cells in vivo. Herein, we constructed recombinant adenovirus encoding human transforming growth factor-beta3 (hTGF-beta3) and practiced it in rat bone marrow-derived mesenchymal stromal cells and articular chondrocytes for cartilage regeneration. Optimal viable transduction and transgenic hTGF-beta3 production were achieved; consequently, positive expression of cartilage marker-collagen type II was enabled in the infected progenitors. We thus conclude that recombinant adenovirus encoding TGF-beta3 gene has been successfully established and validated for cartilage tissue engineering applications.
Hao
Jinghua
J
Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University , Singapore, Republic of Singapore.
Yao
Yongchang
Y
Varshney
Rohan R
RR
Wang
Laicheng
L
Prakash
Celine
C
Li
Hao
H
Wang
Dong-An
DA
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D018014:Gene Transfer Techniques; D006801:Humans; D008297:Male; D008954:Models, Biological; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D053782:Transforming Growth Factor beta3 / Q000378:metabolism*; D019076:Transgenes
D053782:Transforming Growth Factor beta3