title: Alterations in protein secretion caused by metabolic engineering of glycosylation pathways in fungi.
abstract: Due to its natural properties, Trichoderma reesei is commonly used in industry-scale production of secretory proteins. Since almost all secreted proteins are O-glycosylated, modulation of the activity of enzymes of the O-glycosylation pathway are likely to affect protein production and secretion or change the glycosylation pattern of the secreted proteins, altering their stability and biological activity. Understanding how the activation of different components of the O-glycosylation pathway influences the glycosylation pattern of proteins and their production and secretion could help in elucidating the mechanism of the regulation of these processes and should facilitate creation of engineered microorganisms producing high amounts of useful proteins. In this review we focus on data concerning Trichoderma, but also present some background information allowing comparison with other fungal species.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland. jsk@ibb.waw.pl
authors.forename: Joanna S
authors.initials: JS
authors.lastname: Kruszewska
authors.forename: Urszula
authors.initials: U
authors.lastname: Perlińska-Lenart
authors.forename: Wioletta
authors.initials: W
authors.lastname: Górka-Nieć
authors.forename: Jacek
authors.initials: J
authors.lastname: Orłowski
authors.forename: Patrycja
authors.initials: P
authors.lastname: Zembek
authors.forename: Grazyna
authors.initials: G
authors.lastname: Palamarczyk
mesh_terms: D002240:Carbohydrate Sequence; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D005800:Genes, Fungal; D006023:Glycoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D006031:Glycosylation; D008969:Molecular Sequence Data; D015202:Protein Engineering; D014242:Trichoderma / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D006023:Glycoproteins