title: Physiological and morphological effects of genistein against the liver fluke, Fasciola hepatica.
abstract: A study has been carried out to determine the activity of genistein against adult liver fluke, Fasciola hepatica. Flukes were incubated in vitro in genistein at a concentration of 0.27 mg/ml (=1 mM). They ceased to move after 3 h, at which point the experiment was terminated and the specimens prepared for examination by scanning and transmission electron microscopy. Surface changes to the flukes comprised swelling and blebbing, especially in the posterior region of the flukes, and there was particular disruption to the spines, accompanied by some spine loss. Fine structural changes to the tegumental syncytium indicated an accelerated release of secretory bodies at the surface, but a reduction in their production within the cell bodies. Autophagic activity was evident in the tegumental cells, a phenomenon that was also observed in the gastrodermal cells. Disruption to the testis and vitelline follicles was severe, with an apparent block in the normal developmental sequence of the spermatogenic and vitelline cells, respectively. Shell protein production by the vitelline cells was also disrupted. In separate experiments, somatic muscle strips were exposed to concentrations of genistein ranging from 1 microm to 1 mm. There were statistically significant increases in the frequency and/or amplitude of muscle contractions at concentrations of 10 microm, 100 microm and 1 mm. The results suggest that genistein is capable of causing severe morphological and neuromuscular disruption to adult flukes in vitro over a short time-span.
authors.affiliation: Parasite Proteomics and Therapeutics Research Group, School of Biological Sciences, Queen's University of Belfast, 97 Lisburn Road, Belfast, Northern Ireland.
authors.forename: E
authors.initials: E
authors.lastname: Toner
authors.forename: G P
authors.initials: GP
authors.lastname: Brennan
authors.forename: K
authors.initials: K
authors.lastname: Wells
authors.forename: J G
authors.initials: JG
authors.lastname: McGeown
authors.forename: I
authors.initials: I
authors.lastname: Fairweather
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D005210:Fasciola hepatica / Q000187:drug effects* / Q000648:ultrastructure; D019833:Genistein / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009132:Muscles / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D000871:Anthelmintics; D019833:Genistein