DNA-templated click chemistry for creation of novel DNA binding molecules.
We have developed a new methodology for producing new molecules that bind to dsDNA using DNA-templated click chemistry. The click reactions between the minor groove binding peptide and acridine intercalators were accelerated by the addition of dsDNA. Furthermore, the resulting peptide-acridine conjugate showed a slightly stronger binding to dsDNA. These results indicate that the DNA-templated click chemistry is applicable for screening new binding molecules.
Imoto
Shuhei
S
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi, 980-8577, Japan.
Hirohama
Tomoya
T
Nagatsugi
Fumi
F
D000166:Acridines / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000737:chemistry*; D015195:Drug Design; D007364:Intercalating Agents / Q000138:chemical synthesis; D007700:Kinetics; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D013696:Temperature; D014230:Triazoles / Q000737:chemistry
D000166:Acridines; D007364:Intercalating Agents; D010455:Peptides; D014230:Triazoles; D004247:DNA