New methodology to search for DNA binding ligands based on duplex DNA-templated click chemistry.
We have developed a new methodology for searching new molecules that bind to dsDNA using DNA-templated click chemistry. The click reactions between the minor groove binding peptide and acridine intercalators were accelerated by addition of dsDNA. Furthermore, resulting peptide-acridine conjugate showed strong binding to dsDNA. These results indicate that DNA-templated click chemistry is applicable to search new DNA binding peptide-acridine conjugates from random peptide library.
Imoto
Shuhei
S
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai-city, Miyagi, Japan.
Hirohama
Tomoya
T
Nagatsugi
Fumi
F
D000166:Acridines / Q000737:chemistry*; D000480:Alkynes / Q000737:chemistry*; D001671:Biochemistry / Q000379:methods; D004247:DNA / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D008024:Ligands; D009842:Oligopeptides / Q000737:chemistry*; D019151:Peptide Library
D000166:Acridines; D000480:Alkynes; D007364:Intercalating Agents; D008024:Ligands; D009842:Oligopeptides; D019151:Peptide Library; D004247:DNA