[Progress on methods for purity assessment of separated chromosome X- or Y-bearing sperm].
In this review of methods for purity assessment of isolated chromosome X- and Y-bearing sperm, we compared the principles, operating procedures, as well as pros and cons for various methods. We conclude that nested PCR of single sperm will become a conventional and popular method with lower costs, and the method will play a very important role in optimizing the X, Y sorting method, if the sensitivity and accuracy of the method can be increased and the testing time decreased, and promote the new progress in other genetic testing techniques on single sperm.
Guo
Jia-Ming
JM
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China. huaguo1981@yahoo.com.cn
Zhu
Hua-Bin
HB
Wang
Dong
D
Zhang
Lin-Bo
LB
Hao
Hai-Sheng
HS
DU
Wei-Hua
WH
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012743:Sex Preselection; D013077:Sperm Head; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics