[MicroRNA differential expression profile during differentiation of embryonic stem cells towards hepatocytes induced by sodium butyrate].
OBJECTIVE
To explore the expression profile of microRNAs during the course of embryonic stem cells differentiation towards hepatocytes induced by sodium butyrate.


METHODS
Total RNA was extracted from embryonic stem cells on day 0, 6, and 9 during cell differentiation, and microRNA was isolated from the total RNA. Microarray analysis of microRNA expression was performed to detect the different expression levels of microRNA among the indicated time points (day 0, 6, and 9).


RESULTS
Compared with the microRNA expression level on day 0 of cell differentiation, 17 different microRNAs exhibited higher expressions both on day 6 and day 9. Twenty-two and 27 microRNA demonstrated lower expressions on day 6 and day 9, respectively. Further analysis revealed that 15 microRNA among the above microRNAs with significant differential expression may keep close interation with histone deacetylase.


CONCLUSIONS
During the course of embryonic stem cells differentiation towards hepatocytes induced by sodium butyrate, histone deacetylase and its relevant microRNAs may play important roles in cell differentiation.
Chen
Ya-jin
YJ
Department of Hepatobiliary Surgery, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China.
Min
Jun
J
Shang
Chang-zhen
CZ
Ren
Meng
M
Peng
Xiao-xiong
XX
Cao
Jun
J
Chen
Ji-sheng
JS
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism
D002087:Butyrates; D035683:MicroRNAs