[Effect of podophyllotoxin on human gastric cancer cell line SGC 7901].
OBJECTIVE
To investigate the effect of podophyllotoxin on human gastric cancer cell line SGC 7901 cells in vitro and in vivo, and to provide evidence for treatment of gastric cancer with podophyllotoxin.


METHODS
SGC 7901 cells were treated with podophyllotoxin at various concentrations for different time. MTT assay, colony formation assay, and flow cytometry were performed to determine the effect of podophyllotoxin on the growth and apoptosis of SGC 7901 cells in vitro. Furthermore, xenograft experiment was performed to detect the effect of podophyllotoxin on the in vivo tumorigenicity of SGC 7901 cells.


RESULTS
Podophyllotoxin inhibited the growth of SGC 7901 cells in a concentration- and time-dependent manner, decreased the colony formation rate of the cells, induced SGC 7901 cell apoptosis, arrested SGC 7901 cells at G2/M phases of cell cycle, and decreased the volume and weight of SGC 7901 cell xenografts in nude mice.


CONCLUSIONS
Podophyllotoxin inhibits the growth of human gastric cancer SGC 7901 cells, induce the apoptosis of SGC 7901 cells in vitro, and inhibit the in vivo tumorigenicity of SGC 7901 cells, which provides evidence for treatment of gastric cancer using podophyllotoxin.
Zhang
Jie
J
Department of Pharmacy, Third Xiangya Hospital, College of Chemistry and Chemical Engieering, Changsha 410083, Central South University, China.
Zhou
Chun-shan
CS
Liu
Shao
S
Chen
Hao
H
Yang
Chao
C
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011034:Podophyllotoxin / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D011034:Podophyllotoxin