Synthesis and immunological evaluation of self-adjuvanting glycolipopeptide vaccine candidates.
Synthesis of four glycolipids with different number of lauroyl groups on glucose or cellobiose as scaffolds is described. Their immunological evaluations either admixed with or covalently linked to J8, a peptide antigen derived from the C-terminus of the antiphagocytic M-protein of group A streptococcus, are also investigated. Administration of mixtures of J8 and glycolipids to B10BR (H-2(k)) mice induced low-levels of J8-specific IgG antibodies. While glycolipopeptides, in which J8 was covalently linked to the synthetic glycolipids, demonstrated high-levels of antibody titers comparable with the co-administration of these glycolipopeptides with complete Freund's adjuvant, suggesting clearly the strong potency of the synthesized glycolipids as self-adjuvanting moieties.
Fujita
Yoshio
Y
School of Molecular and Microbial Sciences (SMMS), The University of Queensland, St. Lucia 4072, Qld, Australia.
Abdel-Aal
Abu-Baker M
AB
Wimmer
Norbert
N
Batzloff
Michael R
MR
Good
Michael F
MF
Toth
Istvan
I
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000378:metabolism; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology / Q000494:pharmacology; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage* / Q000276:immunology / Q000494:pharmacology; D006017:Glycolipids / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000494:pharmacology; D006020:Glycopeptides / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000494:pharmacology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010587:Phagocytosis; D013291:Streptococcus / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D006017:Glycolipids; D006020:Glycopeptides; D007074:Immunoglobulin G; C014689:streptococcal M protein; D005620:Freund's Adjuvant