Accumulation and localization of cesium in edible mushroom (Pleurotus ostreatus) mycelia.
The characteristics of Cs accumulation and localization in edible mushrooms were examined using the mycelia of Pleurotus ostreatus-Y1. Scanning electron microscope images revealed the existence of white spots, and energy dispersive X-ray microanalyzer analysis indicated the presence of larger amounts of Cs and P in these spots in mycelia cultured on medium containing 25 mM CsCl. The (137)Cs activities in the mycelia were approximately 4-6 times higher than those in water used for (137)Cs elution. Higher Cs concentrations in the sediment fraction including vacuolar pellets were obtained compared to the upper fractions. It was observed that yellowish spots caused by the fluorescence of 4',6-diamidino-2-phenylindole (DAPI)-stained polyphosphate were localized in the mycelia. The higher fluorescence intensity of the yellowish-grained spots was measured in comparison with other regions in the mycelium. These results suggested that Cs in the mycelia was trapped by polyphosphate in vacuoles or other organelles.
Sugiyama
Hideo
H
Department of Environmental Health, National Institute of Public Health, 2-3-6 minami Wako-shi, Saitama 351-0197, Japan. sugiyama@niph.go.jp
Takahashi
Mitsuko N
MN
Terada
Hiroshi
H
Kuwahara
Chikako
C
Maeda
Chika
C
Anzai
Yojiro
Y
Kato
Fumio
F
D002586:Cesium / Q000032:analysis / Q000378:metabolism*; D002588:Cesium Radioisotopes / Q000032:analysis / Q000378:metabolism; D004577:Electron Probe Microanalysis; D025282:Mycelium / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D020076:Pleurotus / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D011122:Polyphosphates / Q000378:metabolism
D002588:Cesium Radioisotopes; D011122:Polyphosphates; D002586:Cesium