title: Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats.
abstract: OBJECTIVE
  To evaluate histopathological alterations of the colon wall in segments with and without intestinal transit, by computer-assisted imaging, and to correlate these with the length of time diversion.
  
  
  METHODS
  Thirty male Wistar rats were subjected to intestinal transit diversion by a proximal colostomy and distal mucosa fistula. The animals were divided into three experimental groups according to how long after the initial surgical procedure they were sacrificed: six, twelve and eighteen weeks. Colon segments with and without transit were subjected to histopathological study. The variables colon crypt length, mucosal ulceration, muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria, vascular congestion, number of caliciform cells, inflammatory grade and degree of inflammation, comparing the two colon segments in the different experimental groups were studied. Intestinal crypt length, muscle layer thickness of the mucosa, submucosa and muscularis propria and caliciform cells were measured by computer-assisted imaging method. Mean equality, variance analysis and correlation tests were used in the statistical analysis, and the significance level was set at 5%.
  
  
  RESULTS
  Comparison between segments with and without transit showed that the latter presented reduced length of colon crypts and increased muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria. There were greater quantities of ulceration of the mucosal and greater degree of inflammation with increasing time without transit. Mucosal ulceration, submucosal vascular congestion, increased thickness of the submucosal and muscularis propria layers, presence of caliciform cells, inflammatory infiltrate and inflammatory grade correlated significantly with the length of time without transit.
  
  
  CONCLUSIONS
  Histological alterations occurred in all layers of the colon wall, in the segments without intestinal transit. Ulcerations in the intestinal mucosa, increased number of caliciform cells, greater vascular congestion of the submucosal layer and inflammatory reaction were related to increasing length of time without transit.
authors.affiliation: USF, Bragança Paulista, Sao Paulo, Brazil.
authors.forename: Marcos Vieira de
authors.initials: MV
authors.lastname: Sousa
authors.forename: Denise Gonçalves
authors.initials: DG
authors.lastname: Priolli
authors.forename: Adriana Valim
authors.initials: AV
authors.lastname: Portes
authors.forename: Izilda Aparecida
authors.initials: IA
authors.lastname: Cardinalli
authors.forename: José Aires
authors.initials: JA
authors.lastname: Pereira
authors.forename: Carlos Augusto Real
authors.initials: CA
authors.lastname: Martinez
mesh_terms: D000818:Animals; D003092:Colitis / Q000473:pathology* / Q000503:physiopathology; D003106:Colon / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery; D003125:Colostomy; D005772:Gastrointestinal Transit / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D007413:Intestinal Mucosa / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery; D008297:Male; D051381:Rats; D017208:Rats, Wistar