Chiral separation of fluvastatin enantiomers by capillary electrophoresis.
An analytical CE method was developed for the enantiomeric purity determination of fluvastatin enantiomers. Fluvastatin enantiomers were separated on an uncoated fused silica with 100 mM-borate solution containing 30 mg/mL of (2-hydroxypropyl)-beta-cyclodextrin (HP-beta-CD) as running buffer and fenoprofen as an internal standard. The linearity was observed within a 400-700 microg/mL concentration range (r(2)>or=0.995) for both fluvastatin enantiomers. The repeatability expressed as coefficient of variation (CV) of the method were 0.96 and 0.92% for (+)-3R, 5S and (-)-3S, 5R-fluvastatin, respectively. The limit of detection and quantification for both fluvastatin enantiomers were 1.5 microg/mL and 2.5 microg/mL, respectively.
Trung
Tran Quoc
TQ
College of Pharmacy, Kangwon National University, Chuncheon 200-701, Korea.
Dung
Phan Thanh
PT
Hoan
Nguyen Ngoc
NN
Kim
Dae Joong
DJ
Lee
Joo Huyn
JH
Kim
Kyeong Ho
KH
D053801:Capillary Electrochromatography; D003505:Cyclodextrins; D004574:Electromagnetic Fields; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000302:isolation & purification*; D000077340:Fluvastatin; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013237:Stereoisomerism; D013696:Temperature
D003505:Cyclodextrins; D005229:Fatty Acids, Monounsaturated; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007211:Indoles; D012996:Solutions; D000077340:Fluvastatin