Sensitive DNA probe for photochemical ligation prepared in click chemistry.
We report a new photosensitive probe, that takes advantage of the electronic structural changes associated with the triazole ring formed in the Cu(I)-catalyzed Huisgen 1,3-dipolar cycloaddition. We succeeded in performing photoligation on the second time scale. Huisgen cycloaddition are employed to various labeling. As an example, we report a procedure for preparing the DNA probe for quick SNP typing.
Ami
Takehiro
T
Innovation Plaza Ishikawa, JST, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Matsumura
Hiroshi
H
Fujimoto
Kenzo
K
D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D010777:Photochemistry; D020641:Polymorphism, Single Nucleotide
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides