title: Adaptation of a Mycobacterium strain to phenanthrene degradation in a biphasic culture system: influence on interfacial area and droplet size.
abstract: A phenanthrene-degrading Mycobacterium sp. strain 6PY1 was grown in an aqueous/organic biphasic culture system with phenanthrene as sole carbon source. Its capacity of degradation was studied during sequential inoculum enrichments, reaching complete phenanthrene degradation at a maximim rate of 7 mg l(-1) h(-1). Water-oil emulsions and biofilm formation were observed in biphasic cultures after four successive enrichments. The factors influencing interfacial area in the emulsions were: the initial phenanthrene concentration, the initial inoculum size, and the silicone oil volume fraction. The results showed that the interfacial area was mainly dependent on the silicone oil/mineral salts medium ratio and the inoculum size.
authors.affiliation: Institut National Polytechnique de Grenoble (INPG), ENSEEG, no. 1130 rue de la Piscine, BP 75, Domaine Universitaire, 38402 Saint-Martin d'Heres Cedex, France. arwa.abdelhay@lepmi.inpg.fr
authors.forename: Arwa
authors.initials: A
authors.lastname: Abdelhay
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Magnin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gondrexon
authors.forename: Stéphane
authors.initials: S
authors.lastname: Baup
authors.forename: John
authors.initials: J
authors.lastname: Willison
mesh_terms: D000222:Adaptation, Physiological; D001431:Bacteriological Techniques / Q000379:methods*; D001673:Biodegradation, Environmental; D018441:Biofilms; D003470:Culture Media / Q000737:chemistry*; D009161:Mycobacterium / Q000378:metabolism*; D010616:Phenanthrenes / Q000378:metabolism*; D012827:Silicone Oils / Q000737:chemistry
chemical_list: D003470:Culture Media; D010616:Phenanthrenes; D012827:Silicone Oils; C031181:phenanthrene