Identification of RNA binding specificity for the TET-family proteins.
The TET-family proteins (TAF15, EWS and TLS) are the RNA binding proteins involved in multiple levels of cellular functions. The RNA binding domain of those proteins is known as the important region for cellular functions. But little is known about the RNA binding specificity of TET-family proteins. In order to investigate the RNA binding properties of the TET-family proteins, we performed electrophoretic mobility shift assay using recombinant Flag-tagged TLS and guanine-rich and RNAs. It was found that TLS binds to human telomeric RNA in the presence of KCl, but not in the presence of LiCl.
Takahama
Kentaro
K
Department of Chemistry, Graduate School of Science, Shizuoka University, Suruga, Shizuoka 422-8529, Japan.
Kino
Katsuhito
K
Arai
Shigeki
S
Kurokawa
Riki
R
Oyoshi
Takanori
T
D000818:Animals; D024202:Electrophoretic Mobility Shift Assay; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry; D006801:Humans; D018021:Lithium Chloride / Q000737:chemistry; D011189:Potassium Chloride / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D034702:RNA-Binding Protein FUS / Q000737:chemistry / Q000378:metabolism*; D016615:Telomere / Q000737:chemistry; D013768:Tetrahymena / Q000235:genetics
D034702:RNA-Binding Protein FUS; D006147:Guanine; D012313:RNA; D011189:Potassium Chloride; D018021:Lithium Chloride