Fast screening procedure for antibiotics in wastewaters by direct HPLC-DAD analysis.
Growing concern about the contamination of wastewaters by antibiotics demands fast but sensitive analytical methodologies, for the screening of a large number of samples. The purpose of this work was to develop a simple methodology, using direct injection of the samples, by HPLC with diode array detection (DAD), for a multiresidue analysis of five antibiotics of different classes. Wastewater from an urban water treatment plant was selected as a model to study possible coelution of interfering compounds. The linearity interval ranged from 40 to 400 microg/L for amoxicillin (Amox), metronidazole (Metro), cefazolin (Cefa), and chloramphenicol (Chloram) and from 20 to 200 microg/L for sulfamethoxazole (Sulfa), with LODs lower than 14 microg/L. Repeatability, expressed by the CV of six repeated injections, ranged from 1 to 8%, while the intermediate precision varied between 2 and 11%. The recovery ranged from 90 to 109%. This method enables the fast screening of a large number of samples, with an expanded uncertainty in the 1-22% range. The advantage of the proposed method is to significantly reduce the number of samples to be analyzed by more complex methods.
Teixeira
Salomé
S
REQUIMTE/Departamento de Engenharia Química, Instituto Superior de Engenharia do Porto, Porto, Portugal.
Delerue-Matos
Cristina
C
Alves
Arminda
A
Santos
Lúcia
L
D000658:Amoxicillin / Q000032:analysis*; D002437:Cefazolin / Q000032:analysis*; D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D008795:Metronidazole / Q000032:analysis*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012722:Sewage / Q000737:chemistry*; D013420:Sulfamethoxazole / Q000032:analysis*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000032:analysis
D012722:Sewage; D014874:Water Pollutants, Chemical; D008795:Metronidazole; D002701:Chloramphenicol; D000658:Amoxicillin; D002437:Cefazolin; D013420:Sulfamethoxazole