title: Production and structural analysis of the polysaccharide secreted by Trametes (Coriolus) versicolor ATCC 200801.
abstract: Trametes versicolor ATCC 200801 secretes 4.1 g L(-1) of exopolysaccharide (EPS) when synthetic minimal medium and low-shear bioreactor cultivation technique are used. Structural and compositional analyses by thin layer chromatography, gas chromatography-mass spectrometry, electrospray ionization tandem mass spectrometry, and nuclear magnetic resonance spectroscopy yielded predominantly glucose and small amounts of galactose, mannose, arabinose, and xylose. The main EPS is composed of beta-1,3/beta-1,6-linked D-glucose molecules which is identical with Schizophyllan but does not possess a triple helical arrangement as secondary structure. Two molar mass fractions were detected by size exclusion chromatography yielding weight-average molecular weights of 4,100 and 2.6 kDa. Protein content varies between 2-3.6% (w/w). The exopolysaccharide is different in the nature of the glycosidic linkage, composition of monosaccharides, protein content, and weight-average molecular weight compared to the well-known polysaccharopeptide (PSP) and polysaccharopeptide Krestin (PSK).
authors.affiliation: Institute of Biochemistry and Biotechnology, Technical University Braunschweig, Spielmannstr. 7, 38106 Braunschweig, Germany. U.Rau@tu-bs.de
authors.forename: Udo
authors.initials: U
authors.lastname: Rau
authors.forename: Anja
authors.initials: A
authors.lastname: Kuenz
authors.forename: Victor
authors.initials: V
authors.lastname: Wray
authors.forename: Manfred
authors.initials: M
authors.lastname: Nimtz
authors.forename: Julika
authors.initials: J
authors.lastname: Wrenger
authors.forename: Hasan
authors.initials: H
authors.lastname: Cicek
mesh_terms: D001089:Arabinose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D046911:Macromolecular Substances / Q000737:chemistry* / Q000378:metabolism*; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D055454:Trametes / Q000378:metabolism*; D014994:Xylose / Q000032:analysis
chemical_list: D046911:Macromolecular Substances; D011134:Polysaccharides; D011506:Proteins; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D008358:Mannose; D005690:Galactose