Human oocyte chromosome analysis: complicated cases and major pitfalls.
Human oocytes that remained unfertilized in programmes of assisted reproduction have been analysed cytogenetically for more than 20 years to assess the incidence of aneuploidy in female gametes. However, the results obtained so far are not indisputable as a consequence of difficulties in evaluating oocyte chromosome preparations. Because of the lack of guidelines, we decided to summarize for the first time, the possible pitfalls in human oocyte chromosome analysis. Therefore, we screened the material from our previous studies and compiled representative, complicated cases with recommendations for their cytogenetic classification. We point out that maturity and size of the oocyte are important parameters and that fixation artefacts, as well as the particular structure of oocyte chromosomes, may predispose one to misinterpretations. Moreover, phenomena related to oocyte activation and fertilization are illustrated and explained. This compilation may help to avoid major problems in future studies and contribute to a more precise, and uniform assessment of human oocyte chromosomes.
Rosenbusch
Bernd
B
Department of Gynecology and Obstetrics, University of Ulm, Prittwitzstrasse 43, 89075 Ulm, Germany. bernd.rosenbusch@uniklinik-ulm.de
Schneider
Michael
M
Michelmann
Hans Wilhelm
HW
D002842:Chromatids / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000378:metabolism*; D004171:Diploidy; D005260:Female; D005306:Fertilization; D006801:Humans; D008297:Male; D008938:Mitosis; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D011123:Polyploidy; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism; D016707:Tissue Fixation
D002843:Chromatin
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