title: Diffusion tensor imaging and fiber tractography of C6 rat glioma.
abstract: OBJECTIVE
  To apply diffusion tensor images using 30 noncollinear directions for diffusion-weighted gradient schemes to characterize diffusion tensor imaging (DTI) features associated with C6 glioma-bearing rat brains, and ideally visualize fiber tractography datasets.
  
  
  METHODS
  Fiber tractographies of normal male Fischer 344 rat brains were constructed from DTI datasets acquired with a 30 noncollinear diffusion gradient scheme. Cultured C6 cell were intracranially injected into the cortex of male Fischer 344 rats. The time course of the tumor growth was monitored with DTI and fiber tractography using diffusion-weighting gradients in 30 noncollinear directions.
  
  
  RESULTS
  Fiber tractographies through the corpus callosum (CC) were easily visualized with the 30-direction gradient scheme, and the fiber trajectories of the motor cortex and striatum were well represented in normal rats. Fiber tractography indicated that the neuronal fibers of the CC were compressed or disappeared by growing C6 glioma, which affected surrounding brain tissue.
  
  
  CONCLUSIONS
  We have demonstrated in this study that fiber tractography with the 30 noncollinear diffusion gradient scheme method can be used to help provide a better understanding regarding the influence of a tumor on the surrounding regions of normal brain tissue in vivo.
authors.affiliation: Small Animal MRI Facility, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA.
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Asanuma
authors.forename: Sabrina
authors.initials: S
authors.lastname: Doblas
authors.forename: Yasvir A
authors.initials: YA
authors.lastname: Tesiram
authors.forename: Debra
authors.initials: D
authors.lastname: Saunders
authors.forename: Rebecca
authors.initials: R
authors.lastname: Cranford
authors.forename: Jamie
authors.initials: J
authors.lastname: Pearson
authors.forename: Andrew
authors.initials: A
authors.lastname: Abbott
authors.forename: Nataliya
authors.initials: N
authors.lastname: Smith
authors.forename: Rheal A
authors.initials: RA
authors.lastname: Towner
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D005910:Glioma / Q000473:pathology*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D009412:Nerve Fibers / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity