Arabidopsis WRKY38 and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense.
Arabidopsis thaliana WRKY38 and WRKY62, encoding two structurally similar type III WRKY transcription factors, are induced in a Nonexpressor of PR Gene1 (NPR1)-dependent manner by salicylic acid (SA) or by virulent Pseudomonas syringae. Disease resistance and SA-regulated Pathogenesis-Related1 (PR1) gene expression are enhanced in the wrky38 and wrky62 single mutants and, to a greater extent, in the double mutants. Overexpression of WRKY38 or WRKY62 reduces disease resistance and PR1 expression. Thus, WRKY38 and WRKY62 function additively as negative regulators of plant basal defense. WRKY38 and WRKY62 interact with Histone Deacetylase 19 (HDA19). Expression of HDA19 is also induced by P. syringae, and the stability of its induced transcripts depends on SA and NPR1 in infected plants. Disruption of HDA19 leads to compromised resistance, whereas its overexpression results in enhanced resistance to P. syringae. Thus, HDA19 has a role opposite from those of WRKY38 and WRKY62 in basal resistance to the bacterial pathogen. Both WRKY38 and WRKY62 are transcriptional activators in plant cells, but their activation activities are abolished by overexpressed HDA19. Interaction of WRKY38 and WRKY62 with HDA19 may act to fine-tune plant basal defense responses.
Kim
Kang-Chang
KC
Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907-2054, USA.
Lai
Zhibing
Z
Fan
Baofang
B
Chen
Zhixiang
Z
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D024202:Electrophoretic Mobility Shift Assay; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D007113:Immunity, Innate / Q000235:genetics; D047468:Immunoprecipitation; D008954:Models, Biological; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D011485:Protein Binding; D044224:Pseudomonas syringae / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D014157:Transcription Factors; C523309:WRKY62 protein, Arabidopsis; C053376:pathogenesis-related proteins, plant; C507152:HDA19 protein, Arabidopsis; D006655:Histone Deacetylases; D020156:Salicylic Acid
PLoS Pathog. 2006 Nov;2(11):e123
17096590
Plant J. 2006 May;46(3):477-91
16623907
Plant J. 2006 Nov;48(4):592-605
17059405
Cell. 2000 Dec 22;103(7):1111-20
11163186
Phytochemistry. 2006 Jul;67(14):1406-13
16839576
Mol Plant Microbe Interact. 2007 Feb;20(2):120-8
17313163
Plant Physiol. 2006 Nov;142(3):1180-92
16963526
EMBO J. 1996 Oct 15;15(20):5690-700
8896462
Plant Cell. 2004 Feb;16(2):319-31
14742872
EMBO J. 2005 Jul 20;24(14):2579-89
15990873
Plant Cell. 2006 May;18(5):1310-26
16603654
Plant Physiol. 1997 Nov;115(3):1021-1027
12223856
Plant Cell. 1995 Nov;7(11):1941-50
8535144
Plant Physiol. 2002 Jun;129(2):706-16
12068113
Plant J. 2001 Jun;26(5):509-22
11439137
Mol Plant Microbe Interact. 2003 Apr;16(4):295-305
12744458
FEBS Lett. 2005 Feb 28;579(6):1545-50
15733871
Plant Cell. 2006 Nov;18(11):3289-302
17114354
Plant Mol Biol. 2000 Jan;42(2):387-96
10794538
Science. 2007 Feb 23;315(5815):1098-103
17185563
Plant J. 1998 Dec;16(6):735-43
10069079
Genetics. 2003 Sep;165(1):399-409
14504245
Curr Opin Plant Biol. 2004 Oct;7(5):491-8
15337090
Plant Cell. 2005 Apr;17(4):1196-204
15749761
Cell. 1997 Jan 10;88(1):57-63
9019406
BMC Plant Biol. 2007 Jan 10;7:2
17214894
Mol Cell Biol. 2007 Apr;27(8):3199-210
17296735
Plant J. 1997 Mar;11(3):605-12
9107046
Plant Mol Biol. 2003 Jan;51(1):21-37
12602888
Plant J. 2003 Apr;34(2):217-28
12694596
Curr Opin Plant Biol. 2007 Aug;10(4):366-71
17644023
Annu Rev Phytopathol. 2005;43:205-27
16078883
Plant J. 2006 Oct;48(1):28-44
16925600
Genes Dev. 1998 Mar 15;12(6):797-805
9512514
Trends Plant Sci. 2000 May;5(5):199-206
10785665
Plant Cell Physiol. 2007 Jun;48(6):833-42
17510065
Curr Opin Plant Biol. 2002 Aug;5(4):325-31
12179966
Plant Cell. 2007 Apr;19(4):1388-402
17468259
Plant J. 2005 Sep;43(6):873-88
16146526
Nature. 2001 Nov 29;414(6863):562-5
11734859
Curr Biol. 1998 Oct 8;8(20):1121-4
9778531
Nature. 2006 Nov 16;444(7117):323-9
17108957
Science. 2006 Jun 9;312(5779):1520-3
16763149
Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):200-5
11134508
Plant Cell. 2003 Mar;15(3):760-70
12615947
Plant Physiol. 2006 Jan;140(1):249-62
16377744
Plant Cell. 2001 Jul;13(7):1527-40
11449049