title: The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism.
abstract: Members of the genus Thermococcus, sulfur-reducing hyperthermophilic archaea, are ubiquitously present in various deep-sea hydrothermal vent systems and are considered to play a significant role in the microbial consortia. We present the complete genome sequence and feature analysis of Thermococcus onnurineus NA1 isolated from a deep-sea hydrothermal vent area, which reveal clues to its physiology. Based on results of genomic analysis, T. onnurineus NA1 possesses the metabolic pathways for organotrophic growth on peptides, amino acids, or sugars. More interesting was the discovery that the genome encoded unique proteins that are involved in carboxydotrophy to generate energy by oxidation of CO to CO(2), thereby providing a mechanistic basis for growth with CO as a substrate. This lithotrophic feature in combination with carbon fixation via RuBisCO (ribulose 1,5-bisphosphate carboxylase/oxygenase) introduces a new strategy with a complementing energy supply for T. onnurineus NA1 potentially allowing it to cope with nutrient stress in the surrounding of hydrothermal vents, providing the first genomic evidence for the carboxydotrophy in Thermococcus.
authors.affiliation: Korea Ocean Research and Development Institute, Ansan, P.O. Box 29, Seoul 425-600, South Korea.
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Lee
authors.forename: Sung Gyun
authors.initials: SG
authors.lastname: Kang
authors.forename: Seung Seob
authors.initials: SS
authors.lastname: Bae
authors.forename: Jae Kyu
authors.initials: JK
authors.lastname: Lim
authors.forename: Yona
authors.initials: Y
authors.lastname: Cho
authors.forename: Yun Jae
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Jeon
authors.forename: Sun-Shin
authors.initials: SS
authors.lastname: Cha
authors.forename: Kae Kyoung
authors.initials: KK
authors.lastname: Kwon
authors.forename: Hyung-Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Cheol-Joo
authors.initials: CJ
authors.lastname: Park
authors.forename: Hee-Wook
authors.initials: HW
authors.lastname: Lee
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Jongsik
authors.initials: J
authors.lastname: Chun
authors.forename: Rita R
authors.initials: RR
authors.lastname: Colwell
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D020745:Genome, Archaeal / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D019712:Thermococcus / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D019641:DNA, Archaeal; D002248:Carbon Monoxide
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