title: RNA aptamers specifically interact with the Fc region of mouse immunoglobulin G.
abstract: We have designed the in vitro selection method to obtain some aptamers such as a general antibody-probing agent, which might bind to the constant regions of mouse immunoglobulin G (IgG) subclasses. As a consequence, one of the selected aptamers found to recognize mouse IgG1, 2a, and 3 subclasses. According to the binding assay, it is suggested that this aptamer recognizes the constant regions of mouse IgG subclass. In addition, this aptamer could recognize the only native form of mouse IgGs but the denatured IgGs. These features show the advantage of the aptamer as an antibody-probing agent rather than the usual secondary antibodies.
authors.affiliation: VALWAY Technology Center, NEC Soft, Ltd., Tokyo, 136-8627, Japan.
authors.forename: Nobuya
authors.initials: N
authors.lastname: Sakai
authors.forename: Hiromi
authors.initials: H
authors.lastname: Masuda
authors.forename: Joe
authors.initials: J
authors.lastname: Akitomi
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Yagi
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Katsunori
authors.initials: K
authors.lastname: Horii
authors.forename: Makio
authors.initials: M
authors.lastname: Furuichi
authors.forename: Iwao
authors.initials: I
authors.lastname: Waga
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015151:Immunoblotting; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism*; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D020349:Surface Plasmon Resonance
chemical_list: D052157:Aptamers, Nucleotide; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G