title: A novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in Drosophila.
abstract: Translational read-through of the UGA stop codon is an evolutionarily conserved feature that most prominently represents the basis of selenoprotein biosynthesis. It requires a specific cis-acting stem loop control element, termed SECIS, which is located in the 3'-untranslated region of eukaryotic selenoprotein mRNAs. In a search for novel factors underlying the SECIS-directed UGA read-through process, we identified an evolutionary conserved GTPase-activating protein, termed GAPsec. We show that the activity of the Drosophila GAPsec (dGAPsec) is necessary to support SECIS-dependent UGA read-through activity in flies and the mouse homolog mGAPsec in mice tissue culture cells. However, selenoprotein biosynthesis is not impaired in flies that lack dGAPsec activity. The results indicate that GAPsec is part of a novel SECIS-dependent translational read-through system that does not involve selenocysteine incorporation.
authors.affiliation: Max-Planck-Institut für biophysikalische Chemie, Abteilung Molekulare Entwicklungsbiologie, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Hirosawa-Takamori
authors.forename: Dmitri
authors.initials: D
authors.lastname: Ossipov
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Novoselov
authors.forename: Anton A
authors.initials: AA
authors.lastname: Turanov
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Vadim N
authors.initials: VN
authors.lastname: Gladyshev
authors.forename: Alain
authors.initials: A
authors.lastname: Krol
authors.forename: Gerd
authors.initials: G
authors.lastname: Vorbrüggen
authors.forename: Herbert
authors.initials: H
authors.lastname: Jäckle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018388:Codon, Terminator / Q000378:metabolism*; D004330:Drosophila / Q000378:metabolism*; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D055029:Inverted Repeat Sequences / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D030781:Organisms, Genetically Modified; D017279:Selenocysteine / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D018388:Codon, Terminator; D029721:Drosophila Proteins; D020690:GTPase-Activating Proteins; D017279:Selenocysteine
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