title: The TRAPP complex: insights into its architecture and function.
abstract: Vesicle-mediated transport is a process carried out by virtually every cell and is required for the proper targeting and secretion of proteins. As such, there are numerous players involved to ensure that the proteins are properly localized. Overall, transport requires vesicle budding, recognition of the vesicle by the target membrane and fusion of the vesicle with the target membrane resulting in delivery of its contents. The initial interaction between the vesicle and the target membrane has been referred to as tethering. Because this is the first contact between the two membranes, tethering is critical to ensuring that specificity is achieved. It is therefore not surprising that there are numerous 'tethering factors' involved ranging from multisubunit complexes, coiled-coil proteins and Rab guanosine triphosphatases. Of the multisubunit tethering complexes, one of the best studied at the molecular level is the evolutionarily conserved TRAPP complex. There are two forms of this complex: TRAPP I and TRAPP II. In yeast, these complexes function in a number of processes including endoplasmic reticulum-to-Golgi transport (TRAPP I) and an ill-defined step at the trans Golgi (TRAPP II). Because the complex was first reported in 1998 (1), there has been a decade of studies that have clarified some aspects of its function but have also raised further questions. In this review, we will discuss recent advances in our understanding of yeast and mammalian TRAPP at the structural and functional levels and its role in disease while trying to resolve some apparent discrepancies and highlighting areas for future study.
authors.affiliation: Department of Biology, Concordia University, Montreal, QC, Canada. msacher@alcor.concordia.ca
authors.forename: Michael
authors.initials: M
authors.lastname: Sacher
authors.forename: Yeon-Gil
authors.initials: YG
authors.lastname: Kim
authors.forename: Arnon
authors.initials: A
authors.lastname: Lavie
authors.forename: Byung-Ha
authors.initials: BH
authors.lastname: Oh
authors.forename: Nava
authors.initials: N
authors.lastname: Segev
mesh_terms: D000818:Animals; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000737:chemistry* / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000378:metabolism
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D021122:Protein Subunits; D033921:Vesicular Transport Proteins; C406327:transport protein particle, TRAPP; D020691:rab GTP-Binding Proteins
references.citation: Genomics. 2000 Oct 15;69(2):242-51
references.pmid: 11031107
references.citation: J Cell Biol. 2000 Oct 16;151(2):289-96
references.pmid: 11038176
references.citation: J Cell Biol. 2000 Oct 30;151(3):551-62
references.pmid: 11062257
references.citation: Mol Biol Cell. 2000 Dec;11(12):4403-11
references.pmid: 11102533
references.citation: Mol Cell Biol. 2001 Jan;21(2):655-62
references.pmid: 11134351
references.citation: Methods Enzymol. 2001;329:132-45
references.pmid: 11210529
references.citation: Methods Enzymol. 2001;329:234-41
references.pmid: 11210539
references.citation: Mol Cell. 2001 Feb;7(2):433-42
references.pmid: 11239471
references.citation: Traffic. 2001 Apr;2(4):268-76
references.pmid: 11285137
references.citation: Am J Hum Genet. 2001 Jun;68(6):1398-407
references.pmid: 11326333
references.citation: Am J Hum Genet. 2001 Jun;68(6):1386-97
references.pmid: 11349230
references.citation: Curr Opin Cell Biol. 2001 Aug;13(4):500-11
references.pmid: 11454458
references.citation: Science. 2001 Jul 27;293(5530):698-702
references.pmid: 11474112
references.citation: J Biol Chem. 2001 Oct 19;276(42):39150-60
references.pmid: 11493604
references.citation: Nature. 2002 Jan 10;415(6868):141-7
references.pmid: 11805826
references.citation: EMBO J. 2002 Mar 15;21(6):1241-7
references.pmid: 11889030
references.citation: J Cell Biol. 2002 May 13;157(4):631-43
references.pmid: 12011112
references.citation: J Cell Sci. 2002 Jul 1;115(Pt 13):2627-37
references.pmid: 12077354
references.citation: Yeast. 2002 Sep 15;19(12):1075-86
references.pmid: 12210902
references.citation: Mol Biol Cell. 2002 Sep;13(9):3336-43
references.pmid: 12221137
references.citation: Curr Genet. 2002 Nov;42(2):85-93
references.pmid: 12478387
references.citation: J Biol Chem. 2002 Dec 20;277(51):49863-9
references.pmid: 12361953
references.citation: FEBS Lett. 2003 Apr 10;540(1-3):77-80
references.pmid: 12681486
references.citation: Mol Biol Cell. 2003 Apr;14(4):1610-23
references.pmid: 12686613
references.citation: Mol Biol Cell. 2003 Jun;14(6):2277-91
references.pmid: 12808029
references.citation: J Biol Chem. 2003 Jun 27;278(26):24033-8
references.pmid: 12700240
references.citation: Clin Genet. 2003 Sep;64(3):235-42
references.pmid: 12919139
references.citation: FEBS Lett. 2003 Aug 28;550(1-3):1-4
references.pmid: 12935876
references.citation: Trends Cell Biol. 2003 Oct;13(10):503-6
references.pmid: 14507476
references.citation: Gene. 2003 Nov 27;320:137-44
references.pmid: 14597397
references.citation: Cell. 1988 Mar 25;52(6):915-24
references.pmid: 3127057
references.citation: Science. 1991 Jun 14;252(5012):1553-6
references.pmid: 1904626
references.citation: Science. 1993 Mar 5;259(5100):1466-8
references.pmid: 8451644
references.citation: Cell. 1993 May 21;73(4):735-45
references.pmid: 8500167
references.citation: Cell. 1994 Jun 17;77(6):895-907
references.pmid: 8004676
references.citation: Hum Mol Genet. 1995 Apr;4(4):709-16
references.pmid: 7633421
references.citation: J Cell Biol. 1995 Sep;130(5):1051-61
references.pmid: 7657691
references.citation: J Cell Biol. 1995 Nov;131(3):583-90
references.pmid: 7593181
references.citation: Yeast. 1995 Sep 30;11(12):1147-55
references.pmid: 8619313
references.citation: Mol Biol Cell. 1995 Dec;6(12):1769-80
references.pmid: 8590804
references.citation: J Biol Chem. 2006 Mar 31;281(13):8898-906
references.pmid: 16439358
references.citation: EMBO J. 2006 Apr 19;25(8):1579-89
references.pmid: 16601699
references.citation: Nat Struct Mol Biol. 2006 Jun;13(6):555-6
references.pmid: 16699513
references.citation: Structure. 2006 Jul;14(7):1157-65
references.pmid: 16843897
references.citation: Genomics. 2006 Aug;88(2):196-203
references.pmid: 16697553
references.citation: Autophagy. 2005 Apr;1(1):37-45
references.pmid: 16874038
references.citation: J Cell Biol. 2006 Jul 31;174(3):359-68
references.pmid: 16880271
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12701-6
references.pmid: 16908848
references.citation: Nat Cell Biol. 2006 Nov;8(11):1263-9
references.pmid: 17041589
references.citation: Cell. 2006 Nov 17;127(4):817-30
references.pmid: 17110339
references.citation: Nature. 2007 Feb 22;445(7130):941-4
references.pmid: 17287728
references.citation: BMC Evol Biol. 2007;7:12
references.pmid: 17274825
references.citation: BMC Evol Biol. 2007;7:29
references.pmid: 17319956
references.citation: Cell. 2007 Jun 1;129(5):865-77
references.pmid: 17540168
references.citation: Mol Biol Cell. 2007 Jul;18(7):2533-41
references.pmid: 17475775
references.citation: J Biol Chem. 2007 Aug 10;282(32):23418-26
references.pmid: 17565980
references.citation: J Cell Biol. 2007 Dec 17;179(6):1179-92
references.pmid: 18086915
references.citation: Biol Direct. 2008;3:2
references.pmid: 18221539
references.citation: Cell. 2008 Jun 27;133(7):1202-13
references.pmid: 18585354
references.citation: Cancer Res. 1999 Nov 15;59(22):5785-92
references.pmid: 10582700
references.citation: Mol Biol Cell. 2000 Jan;11(1):305-23
references.pmid: 10637310
references.citation: Science. 2000 Jul 21;289(5478):444-8
references.pmid: 10903204
references.citation: EMBO J. 2000 Sep 15;19(18):4885-94
references.pmid: 10990452
references.citation: J Cell Biol. 2000 Oct 2;151(1):53-68
references.pmid: 11018053
references.citation: J Cell Biol. 1997 May 5;137(3):563-80
references.pmid: 9151665
references.citation: J Cell Biol. 1997 Jun 30;137(7):1495-509
references.pmid: 9199166
references.citation: Mol Cell Biol. 1998 Feb;18(2):827-38
references.pmid: 9447979
references.citation: EMBO J. 1998 Apr 15;17(8):2156-65
references.pmid: 9545229
references.citation: EMBO J. 1998 May 1;17(9):2494-503
references.pmid: 9564032
references.citation: Genetics. 1998 Jun;149(2):833-41
references.pmid: 9611195
references.citation: Nat Struct Biol. 1998 Sep;5(9):793-802
references.pmid: 9731774
references.citation: Mol Biol Cell. 1998 Oct;9(10):2819-37
references.pmid: 9763446
references.citation: J Biol Chem. 1999 May 21;274(21):15284-91
references.pmid: 10329739
references.citation: Nat Genet. 1999 Aug;22(4):400-4
references.pmid: 10431248
references.citation: Mol Biol Cell. 2005 Jan;16(1):178-92
references.pmid: 15537705
references.citation: Nat Cell Biol. 2005 Jan;7(1):48-55
references.pmid: 15580264
references.citation: Mol Biol Cell. 2005 Feb;16(2):776-93
references.pmid: 15574876
references.citation: Nat Struct Mol Biol. 2005 Jan;12(1):38-45
references.pmid: 15608655
references.citation: Nat Struct Mol Biol. 2005 Feb;12(2):167-74
references.pmid: 15665868
references.citation: J Cell Biol. 2005 Feb 28;168(5):747-59
references.pmid: 15728195
references.citation: EMBO J. 2005 Mar 9;24(5):875-84
references.pmid: 15692564
references.citation: J Cell Sci. 2005 Mar 15;118(Pt 6):1209-22
references.pmid: 15728249
references.citation: EMBO Rep. 2005 Aug;6(8):787-93
references.pmid: 16025134
references.citation: J Biol Chem. 2005 Aug 12;280(32):29233-41
references.pmid: 15951441
references.citation: J Biol Chem. 2005 Sep 30;280(39):33669-78
references.pmid: 16079147
references.citation: Traffic. 2005 Dec;6(12):1183-95
references.pmid: 16262728
references.citation: Nat Struct Mol Biol. 2005 Dec;12(12):1094-100
references.pmid: 16249794
references.citation: J Cell Biol. 2005 Dec 5;171(5):823-33
references.pmid: 16314430