title: Strain-specific sensitivity estimates of Mycobacterium avium subsp. paratuberculosis culture in Greek sheep and goats.
abstract: The requirements for the isolation of Mycobacterium avium subsp. paratuberculosis (Map) may be related to the strain-type [sheep (S)- or cattle (C)-type] and not to the host. The objective of this paper was to estimate and compare strain- and biological sample (faeces or pooled-tissue)--specific sensitivities (Ses) of two solid culture media, Herrold's egg yolk medium (HEYM) and Lowenstein-Jensen (LJ) medium, for the isolation of Map from Greek dairy sheep and goats. From 400 faecal samples collected from sub-clinically infected sheep and goats of four flocks and from 214 pooled-tissue samples (142 from sheep and 72 from goats) collected, at the abattoir, from >1-year-old routinely slaughtered animals, with gross pathology suggestive of paratuberculosis, we isolated 34 Map strains. Of those, by the IS1311 PCR, 18 were categorized into the C-type and nine into the S-type; seven were not typed. We used a Bayesian approach to estimate the strain-specific Ses. SeHEYM-C-faecal=17% (95% credible interval: 7, 40) was higher than SeHEYM-S-faecal=2% (0.3, 11). Also, SeHEYM-C-faecal was higher than SeLJ-C-faecal=4% (1, 12). In pooled-tissue samples, the strain-specific Ses did not differ between the two media.
authors.affiliation: Laboratory of Epidemiology, Biostatistics and Animal Health Economics, University of Thessaly, Karditsa, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Florou
authors.forename: L
authors.initials: L
authors.lastname: Leontides
authors.forename: P
authors.initials: P
authors.lastname: Kostoulas
authors.forename: C
authors.initials: C
authors.lastname: Billinis
authors.forename: M
authors.initials: M
authors.lastname: Sofia
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D002417:Cattle / Q000382:microbiology; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D003470:Culture Media; D005243:Feces / Q000382:microbiology*; D015511:Goat Diseases / Q000175:diagnosis* / Q000382:microbiology; D006041:Goats / Q000382:microbiology; D006115:Greece; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D012756:Sheep / Q000382:microbiology; D012757:Sheep Diseases / Q000175:diagnosis* / Q000382:microbiology; D013045:Species Specificity
chemical_list: D003470:Culture Media