A two-state electronic antigen and an antibody selected to discriminate between these states.
Inspired by biology where pathways are triggered and suppressed by specific binding of two molecules, we realize a functional interface between electronics and biology by replacing one of the pair molecules with a two-state "electronic antigen" device comprising a hydroquinone monolayer assembled on gold, and choosing for the pair molecule an antibody that discriminates between the two electrically selected redox states of the monolayer. Application of an oxidative +0.6 V pulse to the antigen switches it to its benzoquinone state where antibodies bind the layer. A subsequent -0.6 V pulse reduces the monolayer back to the unbinding hydroquinone state, releases the specifically bound antibody molecules, and prevents further binding.
Artzy-Schnirman
Arbel
A
Department of Physics, Technion, Israel Institute of Technology, Haifa 32000, Israel.
Brod
Elad
E
Epel
Malki
M
Dines
Monica
M
Hammer
Tal
T
Benhar
Itai
I
Reiter
Yoram
Y
Sivan
Uri
U
D000818:Animals; D000941:Antigens / Q000737:chemistry*; D015374:Biosensing Techniques; D004563:Electrochemistry / Q000379:methods; D004581:Electronics; D004926:Escherichia coli / Q000378:metabolism; D005098:Exotoxins / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D008956:Models, Chemical; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D010100:Oxygen / Q000737:chemistry; D011549:Pseudomonas / Q000378:metabolism; D013997:Time Factors
D000941:Antigens; D005098:Exotoxins; D006046:Gold; D010100:Oxygen