A novel reagentless amperometric immunosensor based on gold nanoparticles/TMB/Nafion-modified electrode.
A novel reagentless immunosensor was fabricated by immobilization of redox mediator 3,3',5,5'-tetramethylbenzidine (TMB) on the Nafion (Nf) film modified glassy carbon electrode. Gold nanoparticles were assembled onto the TMB/Nafion film modified electrode to provide active sites for the immobilization of antibody molecules. The antibody (anti-MIgG), in the present study, was fixed on the electrode for the rapid detection of antigen molecules (MIgG as a model analyte). The results showed that the immunosensor based on the immobilized TMB redox mediator exhibited good electrochemical response. A good linear relationship between peak current and the concentration of the MIgG was obtained in the concentration range from 4 to 120ng/mL. The detection limit was estimated to be 1ng/ml. Under the optimized conditions, the immunosensor exhibits good sensitivity, reproducibility and stability.
Wu
Ying
Y
Institute of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215123, PR China.
Zheng
Junwei
J
Li
Zheng
Z
Zhao
Yanrong
Y
Zhang
Yu
Y
D001560:Benzidines / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001560:Benzidines; D003432:Cross-Linking Reagents; D005465:Fluorocarbon Polymers; C040402:perfluorosulfonic acid; C021758:3,3',5,5'-tetramethylbenzidine; D006046:Gold