Synthesis of a photoresponsive alpha-dideoxyuridine triphosphate derivative.
The synthesis of a photoresponsive alpha-5-cyanovinyl- 2',3'-dideoxyuridine-5'-triphosphate (alpha dd(C)UTP) is described. A one-pot enzymatic synthesis of photoresponsive alpha-5-cyanovinyl-2',3'-dideoxyuridine (alpha dd(C)U) labeled ODNs with terminal deoxynucleotideyl transferase (TdT) would be a powerful tool for the development of modified long-chain DNA and branched DNA unable to be prepared by DNA synthesizer or enzymatic ligation.
Nitta
Yukari
Y
SC World Inc., 529, Takata, Toyama, 930-0866, Japan.
Ogino
Masayuki
M
Yoshimura
Yoshinaga
Y
Fujimoto
Kenzo
K
D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000096:biosynthesis / Q000737:chemistry; D054306:Dideoxynucleotides / Q000096:biosynthesis* / Q000737:chemistry*; D010777:Photochemistry; D014500:Uracil Nucleotides / Q000096:biosynthesis* / Q000737:chemistry*; D014544:Uridine Triphosphate / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry
D003854:Deoxyribonucleotides; D054306:Dideoxynucleotides; D014500:Uracil Nucleotides; C541621:alpha-5-cyanovinyl-2',3'-dideoxyuridine-5'-triphosphate; D004253:DNA Nucleotidylexotransferase; D014544:Uridine Triphosphate