title: [A follow-up survey of Staphylococcus aureus contamination of commercial raw minced meat at supermarkets and characteristics of isolates].
abstract: A survey of Staphylococcus aureus contamination of commercial raw minced meat at 3 supermarkets in Hyogo Prefecture was conducted over a period of half a year (January to June 2006). In total, the contamination rate was 77.8% (28/36) for beef, 91.7% (33/36) for pork and 91.7% (33/36) for chicken samples. In supermarket A, half or more of the positive samples had MPN values of >/=110/g for all 3 kinds of meat samples. In supermarkets B and C, most of the positive samples were less than 46/g for all 3 kinds of meat samples. Of the 94 isolates examined, 50 (53.2%) belonged to the human biotype, and 44 (46.8%) to animal biotypes. By coagulase typing, 64 (77.1%) of the 83 typable isolates were classified into types V (n=32) and VII (n=32). Seventeen (18.1%) of the 94 isolates produced staphylococcal enterotoxins C (n=16) and B (n=1). Pulse-field gel electrophoresis was applied for epidemiological analysis of the isolates in the 3 supermarkets. In supermarket A, the predominant type shifted during the term of the investigation. In supermarket B, the same type was isolated repeatedly from the meat throughout the investigation. In supermarket C, a variety of genotypes were detected from the meat throughout the investigation.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University. Hyogo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Shimizu
authors.forename: Minematsu
authors.initials: M
authors.lastname: Naka
authors.forename: Junichi
authors.initials: J
authors.lastname: Kawano
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002645:Chickens; D016521:Electrophoresis, Gel, Pulsed-Field; D004768:Enterotoxins / Q000032:analysis; D005516:Food Microbiology; D007564:Japan; D008460:Meat / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013552:Swine
chemical_list: D004768:Enterotoxins