The antioxidant-activity-integrated fingerprint: an advantageous tool for the evaluation of quality of herbal medicines.
The activity-integrated fingerprints of Danshen injections have been established by high-performance liquid chromatography coupled with chemiluminescence detection (HPLC-CL) according to their antioxidants activity scavenging hydrogen peroxide. The authentication and validation of the activity-integrated fingerprints were performed. Then, a data-level information fusion method was employed to capture the chemical and antioxidant information encoded in HPLC-CL fingerprints. Finally, based on the fusion results, the quality of different batches of Danshen injections samples were further evaluated by similarity measure. In comparison with conventional fingerprints, the activity-integrated fingerprints, which simultaneously contained the chemical characteristics and antioxidant activities of constitutions could comprehensively and properly reveal the quality characteristics of the Danshen injections. In conclusion, the antioxidant-activity-integrated fingerprints were suitable for quality control of Danshen injections. This study could clearly demonstrate that the activity-integrated fingerprint was a powerful and meaningful tool to comprehensively improve the quality control of complex herbal medicines.
Chang
Yan-Xu
YX
Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 210009, China.
Ding
Xiao-Ping
XP
Qi
Jin
J
Cao
Jun
J
Kang
Li-Yuan
LY
Zhu
Dan-Ni
DN
Zhang
Bo-Li
BL
Yu
Bo-Yang
BY
D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry*
D000975:Antioxidants; D004365:Drugs, Chinese Herbal