Oligonucleotides direct synthesis on porous silicon chip.
A solid phase oligonucleotide (ON) synthesis on porous silicon (PSi) chip is presented. The prepared Si-OH surface were analyzed by FT-IR and the OH functions were quantified by reaction with 3'-phosphoramidite nucleotide building block. Short ONs were synthesized on the chip surface and the coupling yields evaluated.
De Stefano
Luca
L
Istituto per la Microelettronica e Microsistemi - Unità di Napoli, Consiglio Nazionale delle Ricerche, via P. Castellino 111, I-80131 Napoli, Italy.
De Tommasi
Edoardo
E
Rea
Ilaria
I
Rotiroti
Lucia
L
Giangrande
Luca
L
Oliviero
Giorgia
G
Borbone
Nicola
N
Galeone
Aldo
A
Piccialli
Gennaro
G
D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*
D015345:Oligonucleotide Probes; D012825:Silicon