Disorganization of the Mn4Ca complex of photosystem II by ruthenium red: a thermoluminescence study.
Ruthenium red (RR) is known to be an inhibitor that binds to Ca2+ sites. It releases Ca2+ and Cl(-) together with the extrinsic polypeptide of 17 kDa associated with the oxygen evolving complex of photosystem II. In this work we used thermoluminescence to study S2/3QB(-) and S2QA(-) charge recombination. It is shown that RR produced a deeper inhibition of oxygen evolution compared with the effect of extrinsic polypeptide or Ca2+/Cl(-) depletion. Even though Mn is not released, the Mn cluster is disorganized by RR and the S1-->S2 transition is inhibited.
Gauthier
Alain
A
Groupe de Recherche en Biologie Végétale (GRBV), Université du Québec à Trois-Rivières, C.P. 500 Trois-Rivières, Québec, Canada.
Carpentier
Robert
R
D002118:Calcium / Q000737:chemistry*; D008163:Luminescent Measurements; D008345:Manganese / Q000737:chemistry*; D010100:Oxygen; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000187:drug effects; D012430:Ruthenium Red / Q000737:chemistry* / Q000494:pharmacology
D045332:Photosystem II Protein Complex; D012430:Ruthenium Red; D008345:Manganese; D010100:Oxygen; D002118:Calcium