title: Porous red cell ultrastructure and loss of membrane asymmetry in a novel case of hemolytic anemia.
abstract: Transmission electron microscopic study revealed large pores on the erythrocyte ghost membranes, disrupted cytoskeleton and microcytosis of circulating erythrocytes in a novel case of hemolytic anemia. Greater loss of phosphatidylserine (PS) asymmetry was observed in younger erythrocytes compared with the aged ones in contrast to the normal red cells. Levels of sialylated glycoconjugates, such as glycophorin, measured by the binding of wheat germ agglutinin, showed greater loss upon aging. Such drastic loss of PS asymmetry leads to faster eryptosis, mediated by shedding of glycophorin-containing microvesicles leaving highly PS-exposed erythrocytes accessible to the phagocytes.
authors.affiliation: Clinical Haematology Service, Park Clinic, Kolkata, India.
authors.forename: Debasis
authors.initials: D
authors.lastname: Banerjee
authors.forename: Sutapa
authors.initials: S
authors.lastname: Saha
authors.forename: Sumanta
authors.initials: S
authors.lastname: Basu
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chakrabarti
mesh_terms: D000743:Anemia, Hemolytic / Q000378:metabolism / Q000473:pathology*; D002675:Child, Preschool; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000648:ultrastructure*; D006021:Glycophorins / Q000378:metabolism; D006801:Humans; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods; D010586:Phagocytes / Q000378:metabolism / Q000648:ultrastructure; D010718:Phosphatidylserines / Q000378:metabolism; D014909:Wheat Germ Agglutinins / Q000737:chemistry
chemical_list: D006021:Glycophorins; D010718:Phosphatidylserines; D014909:Wheat Germ Agglutinins