[Construction of 2-dimensional tumor microvascular architecture phenotype in non-small cell lung cancer].
OBJECTIVE
To construct a technological platform of 2-dimensional tumor microvascular architecture phenotype (2D-TAMP) expression.


METHODS
Thirty samples of non-small cell lung cancer (NSCLC) were collected after surgery. The corresponding sections of tumor tissue specimens to the slice of CT perfusion imaging were selected. Immunohistochemical staining,Gomori methenamine silver stain, and electron microscope observation were performed to build a technological platform of 2D-TMAP expression by detecting the morphology and the integrity of basement membrane of microvasculature, microvascular density, various microvascular subtype, the degree of the maturity and lumenization of microvasculature, and the characteristics of immunogenetics of microvasculature.


RESULTS
The technological platform of 2D-TMAP expression was constructed successfully. There was heterogeneity in 2D-TMAP expression of non-small cell lung cancer. The microvascular of NSCLC had certain characteristics.


CONCLUSIONS
2D-TMAP is a key technology that can be used to observe the overall state of micro-environment in tumor growth.
Liu
Jin-kang
JK
Department of Radiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Wang
Xiao-yi
XY
Xiong
Zeng
Z
Zhou
Hui
H
Zhou
Jian-hua
JH
Fu
Chun-yan
CY
Li
Bo
B
D002196:Capillaries / Q000648:ultrastructure*; D002289:Carcinoma, Non-Small-Cell Lung / Q000098:blood supply* / Q000473:pathology; D006801:Humans; D008175:Lung Neoplasms / Q000098:blood supply* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology*; D010641:Phenotype; D012039:Regional Blood Flow / Q000502:physiology