title: Spectral properties of the CP43-deletion mutant of Synechocystis sp. PCC 6803.
abstract: Spectral properties, particularly fluorescence spectra and their time-dependent behavior, were investigated for a mutant of the cyanobacterium Synechocystis sp. PCC 6803 lacking the 43 kDa chlorophyll-protein (CP43, PsbC). Lack of CP43 was confirmed by a size shift of the corresponding gene and by Western blotting. The CP43-deletion mutant grown under heterotrophic conditions accumulated a small amount of photosystem (PS) II, but virtually no PS II fluorescence was observed. A 686-nm fluorescence band was clearly observed by phycocyanin excitation, coming from the terminal pigments of phycobilisomes. In contrast, no PS I fluorescence was detected by phycocyanin excitation when accumulation of PS II components was not proved by a fluorescence excitation spectrum, indicating that energy transfer to PS I chlorophyll a was mediated by PS II chlorophyll a. Direct connection of phycobilisomes with PS I was not suggested. Based on these fluorescence properties, the energy flow in the CP43-deletion mutant cells is discussed.
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Shimada
authors.forename: Tohru
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Seiji
authors.initials: S
authors.lastname: Akimoto
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tomo
authors.forename: Michitaka
authors.initials: M
authors.lastname: Fukuya
authors.forename: Kazunori
authors.initials: K
authors.lastname: Tanaka
authors.forename: Mamoru
authors.initials: M
authors.lastname: Mimuro
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D017353:Gene Deletion; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D046939:Synechocystis / Q000235:genetics / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C083100:photosystem II, chlorophyll binding protein, CP-43
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