Architectonic subdivisions of neocortex in the gray squirrel (Sciurus carolinensis).
Squirrels are highly visual mammals with an expanded cortical visual system and a number of well-differentiated architectonic fields. To describe and delimit cortical fields, subdivisions of cortex were reconstructed from serial brain sections cut in the coronal, sagittal, or horizontal planes. Architectonic characteristics of cortical areas were visualized after brain sections were processed with immunohistochemical and histochemical procedures for revealing parvalbumin, calbindin, neurofilament protein, vesicle glutamate transporter 2, limbic-associated membrane protein, synaptic zinc, cytochrome oxidase, myelin or Nissl substance. In general, these different procedures revealed similar boundaries between areas, suggesting that functionally relevant borders were being detected. The results allowed a more precise demarcation of previously identified areas as well as the identification of areas that had not been previously described. Primary sensory cortical areas were characterized by sparse zinc staining of layer 4, as thalamocortical terminations lack zinc, as well as by layer 4 terminations rich in parvalbumin and vesicle glutamate transporter 2. Primary areas also expressed higher levels of cytochrome oxidase and myelin. Primary motor cortex was associated with large SMI-32 labeled pyramidal cells in layers 3 and 5. Our proposed organization of cortex in gray squirrels includes both similarities and differences to the proposed of cortex in other rodents such as mice and rats. The presence of a number of well-differentiated cortical areas in squirrels may serve as a guide to the identification of homologous fields in other rodents, as well as a useful guide in further studies of cortical organization and function.
Wong
Peiyan
P
Department of Psychology, Vanderbilt University, Nashville, Tennessee, USA.
Kaas
Jon H
JH
D000818:Animals; D005625:Frontal Lobe / Q000033:anatomy & histology; D006179:Gyrus Cinguli / Q000033:anatomy & histology; D009044:Motor Cortex / Q000033:anatomy & histology; D019579:Neocortex / Q000033:anatomy & histology*; D009778:Occipital Lobe / Q000033:anatomy & histology; D012589:Sciuridae / Q000033:anatomy & histology*; D013702:Temporal Lobe / Q000033:anatomy & histology; D014793:Visual Cortex / Q000033:anatomy & histology*
J Comp Neurol. 2008 Mar 10;507(2):1151-68
18175349
Histochemistry. 1982;76(3):281-93
6186642
Brain Behav Evol. 2002;59(5-6):262-72
12207083
J Neurophysiol. 1965 Mar;28:229-89
14283058
Brain Res. 1991 Oct 18;562(1):39-47
1799871
Hear Res. 2003 Jul;181(1-2):116-30
12855370
J Comp Neurol. 2003 Aug 25;463(3):317-40
12820165
J Hirnforsch. 1986;27(2):121-43
3722803
J Comp Neurol. 1973 Jan 15;147(2):163-79
4630151
J Comp Neurol. 1993 Mar 1;329(1):53-67
8384221
J Nutr. 2000 May;130(5S Suppl):1471S-83S
10801962
J Comp Neurol. 1988 Feb 8;268(2):181-203
3360984
Vis Neurosci. 2006 Sep-Oct;23(5):765-78
17020632
Science. 2007 Oct 26;318(5850):655-8
17962567
J Comp Neurol. 1978 May 15;179(2):425-49
417097
J Comp Neurol. 1987 Feb 8;256(2):246-56
3558880
Brain Res. 1971 Mar 5;26(2):277-92
5547178
Anat Embryol (Berl). 1980;159(3):335-60
6970009
Cereb Cortex. 1992 Nov-Dec;2(6):435-43
1477524
J Comp Neurol. 2007 Mar 1;501(1):121-49
17206607
Prog Brain Res. 2001;134:285-95
11702549
J Physiol. 1973 Jul;232(2):275-83
4727082
Cereb Cortex. 1998 Jun;8(4):362-71
9651131
J Chem Neuroanat. 1997 Dec;14(1):1-19
9498163
J Comp Neurol. 1976 Apr 15;166(4):387-401
1270613
Hippocampus. 2007;17(9):709-22
17604355
Eur J Neurosci. 1993 Jul 1;5(7):882-97
8281300
J Chem Neuroanat. 2005 May;29(3):193-208
15820621
Cereb Cortex. 2004 Nov;14(11):1173-84
15142953
Brain Res. 1986 Mar;396(1):77-96
3708387
Physiol Rev. 1983 Jan;63(1):206-31
6401864
Exp Neurol. 1973 Mar-Apr;39(2):270-6
4702821
Anat Embryol (Berl). 1990;181(1):67-73
2407148
Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11459-64
17592148
J Comp Neurol. 2006 Jul 1;497(1):133-54
16680767
J Comp Neurol. 1987 Nov 22;265(4):549-80
2448348
J Comp Neurol. 2001 Aug 13;437(1):17-41
11477594
J Comp Neurol. 1981 Sep 10;201(2):265-83
7287929
Neuropsychologia. 1996 Aug;34(8):741-74
8817506
J Comp Neurol. 1989 Apr 8;282(2):191-205
2496154
J Physiol. 1980 Feb;299:247-76
7381768
J Comp Neurol. 2004 Nov 8;479(2):156-67
15452827
J Comp Neurol. 1999 Aug 30;411(3):503-23
10413783
J Comp Neurol. 1992 Jul 15;321(3):357-72
1380516
Cereb Cortex. 2004 Nov;14(11):1276-86
15166101
J Comp Neurol. 1998 Aug 24;398(2):179-205
9700566
J Comp Neurol. 1982 Apr 10;206(3):227-42
7085930
J Comp Neurol. 1979 Jun 15;185(4):657-78
447876
J Comp Neurol. 2004 Sep 13;477(2):172-87
15300788
Brain Behav Evol. 2000 Oct;56(4):212-34
11155000
Cereb Cortex. 2000 Mar;10(3):206-19
10731217
J Neurophysiol. 1971 May;34(3):437-52
5560040
Science. 1984 Jan 20;223(4633):299-301
6199842
J Neurosci. 1991 Jan;11(1):226-37
1702464
Brain Res. 1969 Feb;12(2):296-320
4185473
Neurol Res. 1979;1(2):203-9
95356
J Comp Neurol. 2004 Nov 22;479(4):360-73
15514982
J Comp Neurol. 1982 Nov 20;212(1):76-88
6294151
Brain Res. 1998 Jan 19;781(1-2):137-47
9507093
J Comp Neurol. 1984 Oct 20;229(2):199-213
6438190
Exp Brain Res. 1993;94(1):1-15
8335065
J Neurophysiol. 2007 May;97(5):3621-38
17376842
Brain Behav Evol. 1970;3(1):155-68
5522341
Brain Res. 1975 Jul 25;93(1):133-9
49213
J Comp Neurol. 1983 Apr 1;215(2):135-53
6853769
Brain Behav Evol. 1994;43(3):162-72
8193908
J Comp Neurol. 1983 May 10;216(2):192-210
6863602
Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1425-9
8434002
J Cogn Neurosci. 1995 Winter;7(1):1-24
23961750
J Comp Neurol. 1981 Feb 1;195(4):603-25
7462444
Histochemistry. 1981;71(1):1-16
6785259
J Comp Neurol. 1956 Oct;105(3):417-537
13385382
Exp Brain Res. 1984;54(2):240-52
6723844
J Comp Neurol. 1998 May 18;394(4):475-95
9590556
Brain Res. 1977 Jul 1;129(2):346-52
69471
Neurosci Res. 2002 Apr;42(4):243-50
11985876
J Comp Neurol. 1979 Apr 1;184(3):473-89
422752
Brain Res. 1979 Feb 9;161(3):539-43
421136
Vis Neurosci. 1994 Jul-Aug;11(4):731-41
7918223
J Comp Physiol Psychol. 1980 Dec;94(6):1087-100
7204699
Brain Res. 1991 Nov 1;563(1-2):293-6
1786543
J Neurosci. 1989 Nov;9(11):3741-52
2479724
Cereb Cortex. 2000 Feb;10(2):192-203
10667987
J Comp Neurol. 1986 Dec 8;254(2):143-65
3794004
Brain Res. 1982 Jun 24;242(2):332-6
6180800
J Comp Neurol. 1975 Nov 15;164(2):247-63
1184785
J Comp Neurol. 2005 Nov 14;492(2):145-77
16196030
J Comp Neurol. 2003 Dec 1;467(1):105-18
14574683
J Comp Neurol. 1989 Mar 15;281(3):426-46
2703555
Exp Brain Res. 2000 Jun;132(4):445-56
10912825
Somatosens Mot Res. 1990;7(3):315-35
2248004
J Neurophysiol. 1997 Jun;77(6):3193-217
9212268
Brain Res. 1980 May;203(1):1-43
6994855
Neuroscience. 2006;138(2):523-35
16426767
Somatosens Mot Res. 1992;9(1):3-36
1317625
Brain Res. 1970 Jan 20;17(2):205-42
4904874
Brain Res. 1997 Aug 29;767(1):148-53
9365028
Anat Embryol (Berl). 1991;184(5):461-8
1741478
Science. 1986 Feb 28;231(4741):995-7
3945815
Exp Neurol. 2002 Apr;174(2):215-29
11922663
J Comp Neurol. 1976 Aug 15;168(4):459-81
939818
J Comp Neurol. 1990 Apr 8;294(2):262-80
2332532
J Comp Neurol. 2001 Jul 2;435(3):379-87
11406819
Vis Neurosci. 1989 Jul;3(1):59-69
2487092
J Comp Neurol. 1990 Oct 22;300(4):593-606
2273095
Braz J Med Biol Res. 1989;22(1):121-38
2758167
J Comp Neurol. 1997 Oct 6;386(4):652-60
9378858
Cereb Cortex. 2007 Dec;17(12):2805-19
17337746
J Neurophysiol. 1986 Dec;56(6):1521-41
3027273
Neuroscience. 2005;133(1):193-207
15893643
Neuroscience. 1991;40(3):725-33
1676494
J Comp Neurol. 1946 Apr;84:221-75
20982805
J Comp Neurol. 2006 Jun 1;496(4):544-55
16572432
J Chem Neuroanat. 1999 Feb;16(2):77-116
10223310
J Comp Neurol. 2007 May 20;502(3):339-57
17366604
J Comp Neurol. 2000 Jul 17;423(1):140-77
10861543
J Comp Neurol. 2000 Jan 24;416(4):521-39
10660882
Behav Biol. 1978 Jan;22(1):1-22
623604
Neuroscience. 1995 Nov;69(1):71-81
8637634
Brain Behav Evol. 1980;17(6):432-53
7437898
J Comp Neurol. 1979 Feb 1;183(3):647-63
103941
Brain Res. 1994 Apr 11;642(1-2):267-80
8032888
Anat Rec (Hoboken). 2008 Oct;291(10):1301-33
18780299
Vision Res. 1988;28(12):1297-302
3256146
J Comp Neurol. 1984 Jul 1;226(3):391-402
6611357
J Comp Neurol. 1980 Sep 1;193(1):187-202
6776164
J Comp Neurol. 1975 Nov 1;164(1):79-94
809494
J Neurosci. 1990 Jan;10(1):73-90
1688937
Prog Brain Res. 2001;134:353-66
11702554
Brain Res Dev Brain Res. 1996 Oct 23;96(1-2):261-76
8922688
Cereb Cortex. 2001 Sep;11(9):849-67
11532890
J Comp Neurol. 1984 Feb 20;223(2):259-301
6200520
Biol Signals. 1994 May-Jun;3(3):127-39
7531563
J Neurosci. 1988 Dec;8(12):4653-61
3199199
J Comp Neurol. 1979 Jan 1;183(1):185-204
102666
J Neurosci. 1983 Aug;3(8):1648-69
6875661
Neurosci Biobehav Rev. 2001 Jul;25(5):395-407
11566478
Brain Res. 1986 Aug 6;379(2):329-41
3742225
Brain Res. 1991 Jan 11;538(2):351-7
2012978
Brain Behav Evol. 1972;6(1):457-83
4122728
J Comp Neurol. 1975 Feb 15;159(4):439-60
1127139
Mol Biol Evol. 2002 Jul;19(7):1053-65
12082125
Brain Res. 1974 Dec 20;82(1):173-7
4611594
Adv Exp Med Biol. 1986;203:83-98
2431606
J Comp Neurol. 1977 May 15;173(2):389-416
853144
Nature. 2001 May 10;411(6834):141-2
11346770
Eur J Neurosci. 1989 May;1(3):222-246
12106154
J Comp Neurol. 1990 Aug 1;298(1):1-22
2170466
Science. 2003 Mar 7;299(5612):1568-72
12595609
J Comp Neurol. 1972 Jul;145(3):273-305
5030907
J Comp Neurol. 1987 Aug 1;262(1):27-45
2442207
J Comp Neurol. 1992 Sep 8;323(2):167-97
1401255
Eur J Neurosci. 2000 Jul;12(7):2425-51
10947821
Behav Brain Res. 2003 Nov 30;146(1-2):3-17
14643455
J Comp Neurol. 2004 Mar 22;471(1):97-111
14983479
J Neurosci. 2003 Feb 15;23(4):1372-82
12598625
Brain Res Rev. 2007 Oct;55(2):285-96
17433837
J Comp Neurol. 1989 Sep 1;287(1):38-63
2477399
Brain Res. 1977 Feb 11;122(1):15-31
837217
Neuroscience. 1988 Feb;24(2):379-431
2452377
J Comp Neurol. 1977 Jan 15;171(2):157-91
64477
J Comp Neurol. 1992 Dec 15;326(3):451-69
1334980
J Comp Neurol. 2000 Apr 10;419(3):306-19
10723007
J Comp Neurol. 1996 Nov 11;375(2):274-88
8915830
J Histochem Cytochem. 2005 Feb;53(2):141-53
15684327
Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):2167-72
14766982
Proc Natl Acad Sci U S A. 1988 Mar;85(6):1998-2002
2450354
Neurosci Res. 1990 Apr;8(1):40-7
2163048
J Comp Neurol. 1983 Dec 10;221(3):329-40
6655087
Brain Res. 1983 May 16;267(2):201-16
6871673
Brain Res. 1979 Jul 27;171(1):11-28
223730
J Comp Neurol. 2005 Apr 18;484(4):458-73
15770654
Neuroscience. 2001;105(3):553-69
11516823
J Comp Neurol. 1998 Oct 26;400(3):349-62
9779940
Brain Res. 1984 Feb 6;292(2):251-60
6692158
Neuroscience. 1990;36(1):165-74
1977100
Brain Res. 1971 Dec 10;35(1):89-106
5002564
Neuroimage. 2004 Jan;21(1):15-26
14741638
Trends Neurosci. 1999 Jun;22(6):242-8
10354599
Exp Brain Res. 1994;100(1):67-84
7813654
J Comp Neurol. 1986 Aug 22;250(4):403-30
3760247
J Comp Neurol. 1983 Jul 10;217(4):390-404
6886060
Brain Res. 1982 Jul;256(3):275-84
6179578