Effect of ursolic acid, a triterpenoid antioxidant, on ultraviolet-B radiation-induced cytotoxicity, lipid peroxidation and DNA damage in human lymphocytes.
Exposure to ultraviolet B (UVB, 280-320) radiation induces the formation of reactive oxygen species (ROS) in the biological system. In this study, we examined the protective effect of ursolic acid on UVB-induced lipid peroxidation and oxidative DNA damage with reference to alterations in cellular antioxidant status in human lymphocytes. Series of in vitro tests (hydroxyl radical, superoxide, nitric oxide, DPPH and ABTS radical scavenging assays) demonstrates antioxidant property of ursolic acid in our study. Treatment of lymphocytes with ursolic acid alone (at 10 microg/mL) gave no significant change in cell viability, thiobarbituric acid reactive substances (TBARSs), lipid hydroperoxides (LHPs), % tail DNA and tail moment when compared with normal lymphocytes. UVB-exposure significantly increased TBARS, LHP and % tail DNA, tail moment; decreased % cell viability and antioxidant status in irradiated lymphocytes. Treatment with ursolic acid 30 min prior to UVB-exposure resulted in a significant decline of TBARS, LHP, % tail DNA and tail moment and increased % cell viability as ursolic acid concentration increased. Based on our results we conclude that ursolic acid, a dietary antioxidant, mediates its protective effect through modulation of UVB-induced reactive oxygen species.
Ramachandran
S
S
Department of Biochemistry & Biotechnology, Annamalai University, Annamalainagar 608 002, Chidambaram, Tamilnadu, India.
Prasad
N Rajendra
NR
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000528:radiation effects; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D008968:Molecular Conformation; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*; D014466:Ultraviolet Rays; D000097245:Ursolic Acid
D000975:Antioxidants; D017665:Hydroxyl Radical; D014315:Triterpenes; D000097245:Ursolic Acid