title: Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments.
abstract: Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from DGGE bands by approximately 23%.
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff, Wales, UK. OsullivanLA@Cardiff.ac.uk
authors.forename: Louise A
authors.initials: LA
authors.lastname: O'Sullivan
authors.forename: Gordon
authors.initials: G
authors.lastname: Webster
authors.forename: John C
authors.initials: JC
authors.lastname: Fry
authors.forename: R John
authors.initials: RJ
authors.lastname: Parkes
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Weightman
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics*; D001419:Bacteria / Q000145:classification / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D019641:DNA, Archaeal / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers; D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S