Growing bacteria on solid media.
Detailed protocols are provided for titering and isolating bacterial colonies by serial dilutions, or alternatively by streaking or spreading a plate. Support protocols describe replica plating as well as methods for storing strains as agar stabs or frozen glycerol stocks.
Lech
K
K
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Brent
R
R
D001431:Bacteriological Techniques / Q000295:instrumentation; D002999:Clone Cells; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D005782:Gels; D007202:Indicators and Reagents; D011309:Preservation, Biological / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
D003470:Culture Media; D005782:Gels; D007202:Indicators and Reagents