title: Functional expression of IgA receptor FcalphaRI on human platelets.
abstract: FcalphaRI (CD89) is a human IgA FcR expressed on cells of myeloid lineage such as neutrophils, monocytes, tissue macrophages, eosinophils, and subpopulations of dendritic cells. FcalphaRI mediates cell activation through Src family kinases and downstream tyrosine-based phosphorylation pathways. However, the role of IgA and the expression and role of its cognate receptor FcalphaRI (CD89) in platelet activation are undefined. In the current study, we demonstrate that human platelets express FcalphaRI mRNAs and proteins. Furthermore, we show that the platelet FcalphaRI is associated with the FcR gamma-chain, and cross-linking of FcalphaRI leads to Syk phosphorylation. Clustering of FcalphaRI induces pre-mRNA splicing and protein production of tissue factor and IL-1beta, suggesting novel roles for human platelet FcalphaRI and serum IgA in thrombosis and inflammation.
authors.affiliation: Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, 202 Shelby Interdisciplinary Biomedical Science Building, 1825 University Boulevard, Birmingham, AL 35294-2182, USA.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.forename: Fenglong
authors.initials: F
authors.lastname: Xie
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Gibson
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Edberg
authors.forename: Robert P
authors.initials: RP
authors.lastname: Kimberly
authors.forename: Jianming
authors.initials: J
authors.lastname: Wu
mesh_terms: D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D003432:Cross-Linking Reagents; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000276:immunology* / Q000378:metabolism; D047468:Immunoprecipitation; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D010766:Phosphorylation; D015539:Platelet Activation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D012322:RNA Precursors / Q000235:genetics; D012326:RNA Splicing / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D011961:Receptors, Fc / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000072377:Syk Kinase; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D003432:Cross-Linking Reagents; C036832:Fc(alpha) receptor; D007070:Immunoglobulin A; D053583:Interleukin-1beta; D047908:Intracellular Signaling Peptides and Proteins; D012322:RNA Precursors; D012333:RNA, Messenger; D011961:Receptors, Fc; D013925:Thromboplastin; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase
references.citation: Lupus. 2003;12(2):112-6
references.pmid: 12630755
references.citation: J Cell Biol. 2001 Aug 6;154(3):485-90
references.pmid: 11489912
references.citation: Cell. 2005 Aug 12;122(3):379-91
references.pmid: 16096058
references.citation: Nature. 1998 Feb 5;391(6667):591-4
references.pmid: 9468137
references.citation: Immunol Today. 1990 Jan;11(1):13-20
references.pmid: 2405873
references.citation: Chem Immunol. 1992;51:33-64
references.pmid: 1533129
references.citation: Blood. 2000 Sep 1;96(5):1798-807
references.pmid: 10961879
references.citation: Lupus. 1998;7 Suppl 2:S48-51
references.pmid: 9814673
references.citation: Mamm Genome. 1999 Feb;10(2):154-60
references.pmid: 9922396
references.citation: Immunogenetics. 2004 Jan;55(10):712-6
references.pmid: 14647992
references.citation: Curr Biol. 1997 Aug 1;7(8):615-8
references.pmid: 9259559
references.citation: Blood. 1999 Apr 15;93(8):2543-51
references.pmid: 10194433
references.citation: Curr Med Chem. 2007;14(20):2170-80
references.pmid: 17691955
references.citation: J Biol Chem. 1999 Oct 8;274(41):29019-24
references.pmid: 10506151
references.citation: J Immunol. 2007 Mar 15;178(6):3973-82
references.pmid: 17339498
references.citation: Rheumatology (Oxford). 2006 May;45(5):645-6; author reply 646
references.pmid: 16492682
references.citation: J Exp Med. 2006 Oct 30;203(11):2433-40
references.pmid: 17060476
references.citation: J Rheumatol. 1998 Apr;25(4):675-80
references.pmid: 9558168
references.citation: J Biol Chem. 2001 Apr 20;276(16):12999-3006
references.pmid: 11152687
references.citation: Arterioscler Thromb Vasc Biol. 2004 Jun;24(6):1015-22
references.pmid: 15117736
references.citation: Annu Rev Immunol. 2003;21:177-204
references.pmid: 12524384
references.citation: Lupus. 2000;9(1):33-41
references.pmid: 10713645
references.citation: J Thromb Haemost. 2003 Sep;1(9):1897-905
references.pmid: 12941029
references.citation: Biochem Biophys Res Commun. 2000 Oct 14;277(1):27-36
references.pmid: 11027634
references.citation: Nat Med. 2002 Nov;8(11):1227-34
references.pmid: 12411949
references.citation: J Immunol. 1998 Oct 15;161(8):4340-6
references.pmid: 9780211
references.citation: J Immunol. 1999 Apr 1;162(7):4311-8
references.pmid: 10201963
references.citation: Clin Exp Nephrol. 2006 Dec;10(4):268-73
references.pmid: 17186331
references.citation: Hematol Oncol Clin North Am. 2007 Feb;21(1):115-21
references.pmid: 17258122
references.citation: J Immunol. 1999 Feb 15;162(4):2146-53
references.pmid: 9973489
references.citation: Semin Thromb Hemost. 2005;31(4):381-92
references.pmid: 16149014
references.citation: Br J Haematol. 2001 Nov;115(2):451-9
references.pmid: 11703349
references.citation: Arthritis Rheum. 2003 Oct;48(10):2741-9
references.pmid: 14558078
references.citation: J Biol Chem. 1997 Sep 19;272(38):23528-31
references.pmid: 9295288
references.citation: J Infect Dis. 1991 Jun;163(6):1177-84
references.pmid: 2037782
references.citation: Trends Cardiovasc Med. 2004 Jan;14(1):18-22
references.pmid: 14720470
references.citation: Immunology. 2005 May;115(1):74-84
references.pmid: 15819699
references.citation: Thromb Res. 2004;113(6):379-85
references.pmid: 15226092
references.citation: Nephron. 1989;51(1):25-8
references.pmid: 2521695
references.citation: J Biol Chem. 1995 Dec 15;270(50):29781-7
references.pmid: 8530370
references.citation: Clin Exp Immunol. 1999 Sep;117(3):574-9
references.pmid: 10469065
references.citation: EMBO J. 1997 May 1;16(9):2333-41
references.pmid: 9171347
references.citation: Ann Rheum Dis. 1999 Oct;58(10):623-9
references.pmid: 10491361
references.citation: Semin Thromb Hemost. 1991 Oct;17(4):422-5
references.pmid: 1803512
references.citation: Immunology. 2004 Oct;113(2):178-86
references.pmid: 15379978
references.citation: Nat Med. 2000 Jun;6(6):680-5
references.pmid: 10835685
references.citation: Nature. 2003 Jun 5;423(6940):614-20
references.pmid: 12768205
references.citation: J Biol Chem. 1994 Dec 23;269(51):32435-40
references.pmid: 7798242
references.citation: Circ Res. 2007 Jan 5;100(1):27-40
references.pmid: 17204662
references.citation: Lupus. 1999;8(4):263-8
references.pmid: 10413203
references.citation: Autoimmun Rev. 2006 Dec;6(2):104-6
references.pmid: 17138253
references.citation: Blood. 2007 Jun 15;109(12):5242-50
references.pmid: 17347408
references.citation: J Biol Chem. 1999 Mar 12;274(11):7216-25
references.pmid: 10066783
references.citation: Blood. 2001 Oct 15;98(8):2442-7
references.pmid: 11588041
references.citation: Proc Natl Acad Sci U S A. 1980 Jun;77(6):3640-4
references.pmid: 6932039
references.citation: Arterioscler Thromb Vasc Biol. 2006 Dec;26(12):2763-8
references.pmid: 17008591
references.citation: Neurology. 1998 Sep;51(3 Suppl 3):S9-14
references.pmid: 9744824
references.citation: Am J Med Sci. 1999 Jul;318(1):55-60
references.pmid: 10408762
references.citation: FEBS Lett. 2003 Jan 30;535(1-3):205-9
references.pmid: 12560105
references.citation: Blood. 1993 Feb 15;81(4):988-93
references.pmid: 8428005
references.citation: N Engl J Med. 1992 Jan 23;326(4):242-50
references.pmid: 1727977
references.citation: Am J Obstet Gynecol. 2001 Sep;185(3):748-53
references.pmid: 11568809
references.citation: Haemostasis. 1988;18(1):29-36
references.pmid: 3047021
references.citation: Curr Vasc Pharmacol. 2003 Jun;1(2):157-69
references.pmid: 15320841
references.citation: J Immunol. 1996 Jun 1;156(11):4442-8
references.pmid: 8666819
references.citation: Immunology. 2004 Feb;111(2):204-11
references.pmid: 15027906