Photoinduced excess electron injection into DNA duplexes containing mismatched base pairs.
A series of DNA containing photoinduced electron donors and mismatched DNA base pairs have been prepared and applied for the chemical investigation of excess electron transfer (EET) in the duplex DNA. As the electron donors, phenothiazine (PTZ) with a flexible linker was tethered to the 5'-end or in the middle of the sequences, or diaminostilbene (DAS) was covalently linked to form a hairpin structure. The presence of mismatched base pair lowered EET efficiency in the DAS-capped DNA hairpins, on the other hand, efficient EET beyond the mismatch site was observed in the PTZ-conjugated DNA.
Ito
Takeo
T
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Kyoto Daigaku Katsura, Kyoto 615-8510, Japan. takeoit@scl.kyoto-u.ac.jp
Kondo
Akiko
A
Hayashi
Aiko
A
Uchida
Tsukasa
T
Tanabe
Kazuhito
K
Nishimoto
Sei-ichi
S
D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D004579:Electron Transport; D004583:Electrons; D010640:Phenothiazines / Q000737:chemistry*; D013267:Stilbenes / Q000737:chemistry*
D010640:Phenothiazines; D013267:Stilbenes; C039607:4,4'-diaminostilbene; D004247:DNA