title: Techniques for bacterial cell culture: media preparation and bacteriological tools.
abstract: Basic information for the culture of bacteria is presented in this appendix, using Escherichia coli as a representative organism. The techniques and recipes should be readily transferrable. Specifics are given for liquid and solid media, rich and minimal media, stab agar, as well as a description of the tools required to inoculate and culture bacteria.
authors.affiliation: Massachusetts General Hospital and Harvard, Medical School Boston, Massachusetts, USA.
authors.forename: K
authors.initials: K
authors.lastname: Lech
authors.forename: R
authors.initials: R
authors.lastname: Brent
mesh_terms: D000362:Agar; D001431:Bacteriological Techniques / Q000295:instrumentation; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D005782:Gels; D012996:Solutions
chemical_list: D003470:Culture Media; D005782:Gels; D012996:Solutions; D000362:Agar