title: Transcriptional regulation of Corynebacterium glutamicum methionine biosynthesis genes in response to methionine supplementation under oxygen deprivation.
abstract: Expression at the mRNA level of six methionine biosynthesis genes in Corynebacterium glutamicum cells under oxygen-deprived conditions was repressed by supplementation of medium with methionine. The repression was not observed in a mutant deficient in the TetR-type transcriptional repressor McbR. Analysis of transcriptional start sites of the methionine biosynthesis genes confirmed that McbR binding motifs exist in the promoter regions of all genes repressed by methionine supplementation. Furthermore, electrophoretic mobility shift assays revealed that not only S-adenosylhomocysteine but also S-adenosylmethionine affects binding of McbR to the promoter region of metY, suggesting that both of these methionine metabolites are involved in the regulation of methionine biosynthesis genes.
authors.affiliation: Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Suda
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Teramoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Imamiya
authors.forename: Masayuki
authors.initials: M
authors.lastname: Inui
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yukawa
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D053898:Biosynthetic Pathways; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D008715:Methionine / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; D008715:Methionine; D010100:Oxygen