Introduction of linkers into PNA for versatile applications.
PNA (peptide nucleic acid) is widely used as a DNA recognition tool. In this study, we introduced linkers in the middle of PNAs to expand their applicability. By means of Tm measurement and gel-shift assay, it was confirmed that these PNA can recognize double stranded DNA through efficient invasion.
Aiba
Yuichiro
Y
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Komiyama
Makoto
M
D004247:DNA / Q000737:chemistry; D024202:Electrophoretic Mobility Shift Assay; D009691:Nucleic Acid Denaturation; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D013696:Temperature
D020135:Peptide Nucleic Acids; D004247:DNA