title: Changes in biomechanical parameters during heart perfusion and after midazolam pre-medication--experimental pilot study.
abstract: BACKGROUND
  Midazolam is a frequently used benzodiazepine in anaesthesiology and intensive care.
  
  
  OBJECTIVE
  The aim of pilot study was to monitor its effect during heart perfusion in the laboratory rat.
  
  
  METHODS
  The same groups of animals (n = 10). The 1(st) group was treated with midazolam in a dose of 0.5mg/kg i.p. The 2(nd) group was a placebo. After i.p. administration of heparine injection of 500 IU dose, the hearts were excised and perfused (modified Langendorf's method). Working schedule: stabilization/ischaemia/reperfusion proceed at intervals of 20/30/60 min. Monitored parameters in isolated heart: left ventricle pressure (LVP), end-diastolic pressure (LVEDP), contractility (+dP/dt(max)).
  
  
  RESULTS
  The treated hearts showed improved postischemic recovery, reaching LVP values of 92 +/- 6 % at the end of the reperfusion, placebo only 61 +/- 7 %. In placebo hearts LVEDP rose from 10.0 +/- 0.5 mmHg to 43 +/- 4 mmHg after, in treated animals only about 25 mmHg. The treated hearts improved +dP/dt(max) recovery during reperfusion to 91 +/- 8 %. These values were significantly greater than those obtained from the placebo hearts.
  
  
  CONCLUSIONS
  Positive changes in monitored parameters were found in this experimental pilot study. We conclude that the administration of midazolam in laboratory rats has a cardioprotective potential against ischemia-reperfusion induced injury.
authors.affiliation: Department of Physiology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic. bartosil@tunw.upol.cz
authors.forename: Lenka
authors.initials: L
authors.lastname: Bartosikova
authors.forename: Jiri
authors.initials: J
authors.lastname: Necas
authors.forename: Tomas
authors.initials: T
authors.lastname: Bartosik
authors.forename: Petr
authors.initials: P
authors.lastname: Frana
authors.forename: Martin
authors.initials: M
authors.lastname: Pavlik
mesh_terms: D000818:Animals; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008874:Midazolam / Q000494:pharmacology*; D009200:Myocardial Contraction / Q000187:drug effects; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D011229:Preanesthetic Medication; D051381:Rats; D017208:Rats, Wistar; D016277:Ventricular Function, Left / Q000187:drug effects
chemical_list: D006993:Hypnotics and Sedatives; D008874:Midazolam