title: [Three-dimensional organization of telomeres in the Drosophila melanogaster salivary glands nuclei].
abstract: The 3D-FISH was employed to investigate the telomere topology in polytene nuclei of salivary glands of Drosophila melanogaster. The majorities of telomeres in polytene nuclei of salivary glands in Drosophila strain y(2-717) are localized in the nuclear central area and have no contacts with nuclear membrane. In females of this strain, ectopic contacts between telomeres occur at 25 % higher frequency than in males. HeT-A DNA in y(2-717alk3-2) strain, which is a derivative of y(2-717) carrying an inversion between 1D and 13C bands, is found in region 13 of X chromosome. The frequency of ectopic contacts of telomeres in y(2-717alk3-2) males is 10 % higher than that in y(2-717) strain. The number of ectopic contacts can be significantly different in independent experiments, possibly indicating the role of random factors in the contact formation.
authors.forename: L P
authors.initials: LP
authors.lastname: Zakharenko
authors.forename: M P
authors.initials: MP
authors.lastname: Perepelkina
authors.forename: S
authors.initials: S
authors.lastname: Mai
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D004331:Drosophila melanogaster / Q000648:ultrastructure*; D005260:Female; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D018613:Microscopy, Confocal; D012469:Salivary Glands / Q000648:ultrastructure*; D016615:Telomere / Q000648:ultrastructure*; D014960:X Chromosome / Q000648:ultrastructure