title: Exceptional fluorescence and hybridization properties of a phenylpyrrolocytosine in peptide nucleic acid.
abstract: A phenylpyrrolocytosine derivative possessing bis-(ortho-aminoethoxy) substitution has been designed, synthesized and incorporated into PNA resulting in oligomers that demonstrate increased binding affinity for DNA targets. The phenylpyrrolocytosine is an exceptionally fluorescent modified nucleobase possessing a high quantum yield while the fluorescence intensity displays useful environmental sensitivity.
authors.affiliation: Department of Chemistry, The University of Western Ontario, London, Ontario, Canada.
authors.forename: Filip
authors.initials: F
authors.lastname: Wojciechowski
authors.forename: Robert H E
authors.initials: RH
authors.lastname: Hudson
mesh_terms: D003596:Cytosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D011758:Pyrroles / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids; D011758:Pyrroles; D003596:Cytosine; D004247:DNA