A new storage medium for an avulsed tooth.
OBJECTIVE
The purpose of this study is to determine the efficacy of egg white in maintaining the viability of human periodontal ligament (PDL) cells on avulsed teeth.


METHODS
The experimental media were: egg white, milk, Hanks' Balanced Salt Solution (HBSS) as the positive control, and tap water as the negative control. The storage times were 1, 2, 4, 8, and 12 hours. Extracted premolar teeth of healthy individuals were rinsed in the media. After trypsinization and subsequent treatment in collagenase, cell viability was determined using trypan blue staining. The two-way analysis of variance (ANOVA) statistical test was used to compare the results among different media.


RESULTS
There was no difference in the cell viability between egg white and HBSS media, but there was a statistically significant difference between the viability of PDL cells in egg white medium in comparison with milk (P<0.05) and water (P<0.05).


CONCLUSIONS
Egg white could be suggested as a suitable storage medium. Its principle advantage is its availability.
Khademi
Abbas Ali
AA
Department of Endodontics, Isfahan University, Iran.
Saei
Saeed
S
Mohajeri
Mohammad Reza
MR
Mirkheshti
Nooshin
N
Ghassami
Fatima
F
Torabi nia
Nakisa
N
Alavi
Sayyed Ali
SA
D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival; D004529:Egg White; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007552:Isotonic Solutions; D008892:Milk; D019188:Organ Preservation Solutions; D009994:Osmolar Concentration; D010513:Periodontal Ligament / Q000166:cytology / Q000502:physiology*; D014084:Tooth Avulsion / Q000628:therapy*
C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions