Large-scale gene trapping in C57BL/6N mouse embryonic stem cells.
We report the construction and analysis of a mouse gene trap mutant resource created in the C57BL/6N genetic background containing more than 350,000 sequence-tagged embryonic stem (ES) cell clones. We also demonstrate the ability of these ES cell clones to contribute to the germline and produce knockout mice. Each mutant clone is identified by a genomic sequence tag representing the exact insertion location, allowing accurate prediction of mutagenicity and enabling direct genotyping of mutant alleles. Mutations have been identified in more than 10,000 genes and show a bias toward the first intron. The trapped ES cell lines, which can be requested from the Texas A&M Institute for Genomic Medicine, are readily available to the scientific community.
Hansen
Gwenn M
GM
Lexicon Pharmaceuticals Incorporated, The Woodlands, Texas 77381, USA. ghansen@lexpharma.com
Markesich
Diane C
DC
Burnett
Michael B
MB
Zhu
Qichao
Q
Dionne
Karen M
KM
Richter
Lizabeth J
LJ
Finnell
Richard H
RH
Sands
Arthur T
AT
Zambrowicz
Brian P
BP
Abuin
Alejandro
A
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002460:Cell Line; D002678:Chimera; D002999:Clone Cells; D004622:Embryo, Mammalian / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D007438:Introns; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D035683:MicroRNAs; D016254:Mutagenesis, Insertional
D035683:MicroRNAs
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