title: Calibration: sampling density and spatial resolution.
abstract: This unit presents a discussion of procedures for measuring the sampling density and spatial resolution of a quantitative microscope system. These two independent quantities are fundamental characteristics of a system that must be known for proper processing and interpretation of digitized microscope images and of measurements extracted from such images.
authors.affiliation: Delft University of Technology, Delft, The Netherlands.
authors.forename: I T
authors.initials: IT
authors.lastname: Young
mesh_terms: D000465:Algorithms; D002138:Calibration; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000379:methods; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D012984:Software