Integrated microfluidic system for electrochemical sensing of urinary proteins.
This study reports a new microfluidic system integrated with a microfluidic control module and a micro electrochemical module for detection of urinary proteins. The integrated microsystem can automatically detect proteins in urine with a high sensitivity. The microfluidic control module consists of a new two-way, spiral-shape micropump which can transport the urine samples to the sensing regions. The net ionic charges of the protein samples can be detected while the samples flow through the sensing region of the micro electrochemical module. Two major urinary proteins including lysozyme and albumin are detected in a multiple-channel layout with little human intervention and are analyzed in a short period of time, while only consuming a 100-mul urine sample. The developed microfluidic system could lead to a convenient, yet crucial, platform for chemical and biological detection and diagnosis.
Liu
Cheng-Yu
CY
Department of Engineering Science, National Cheng Kung University, Tainan, 701, Taiwan.
Rick
John
J
Chou
Tse-Chuan
TC
Lee
Huei-Huang
HH
Lee
Gwo-Bin
GB
D000418:Albumins / Q000032:analysis*; D000419:Albuminuria / Q000652:urine*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D009113:Muramidase / Q000652:urine*; D012680:Sensitivity and Specificity
D000418:Albumins; D009113:Muramidase