Suppression of bcr-abl mRNA by chemically modified siRNA.
Synthesis of 21 nt siRNAs bearing chemically modified dangling ends with a novel nucleobase was achieved. Evaluation of gene silencing of bcr-abl chimeric gene derived from Philadelphia chromosome by thus obtained chemically modified siRNAs was performed using human leukaemia cell line K-562 and resulted in efficient suppression of the targeted gene. siRNAs whose sense strands were modified with a novel base were found to be more effective than a native siRNA and that siRNAs whose antisense strands were modified with a novel base showed largely decreased silencing effects on the contrary.
Murao
Satoshi
S
Faculty of Humanity Oriented Technology, Kinki University, Iizuka, Fukuoka 820-8555, Japan.
Diala
Irmina
I
Fujii
Masayuki
M
D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D016044:Fusion Proteins, bcr-abl / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000737:chemistry*
C541632:N4-(N'-phenylcarbamoyl)-2'-deoxycytidine; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D003841:Deoxycytidine; D016044:Fusion Proteins, bcr-abl