Enhanced amplification of polymerase chain reaction by addition of cosolutes derived from a cellular compatible solute.
We designed and synthesized artificial compatible solutes which can not only decrease the melting temperature of DNA duplexes dependent of their chemical structures but also improve the amplification of highly stable genome DNA sequence.
Koumoto
Kazuya
K
FIBER, Konan University, Higashinada-ku, Kobe, 658-8501, Japan.
Ochiai
Hirofumi
H
Sugimoto
Naoki
N
D001622:Betaine / Q000031:analogs & derivatives*; D004247:DNA / Q000737:chemistry; D006801:Humans; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction
D001622:Betaine; D004247:DNA