Granzyme A causes detachment of alveolar epithelial A549 cells accompanied by promotion of interleukin-8 release.
Granzyme A (GrA) is a serine protease produced in cytotoxic lymphocytes, lung epithelial cells (alveolar type-II cells), and alveolar macrophages. In the present study, recombinant rat GrA (rGrA) was found to cause rounding and detachment of an alveolar type-II epithelial cell line, A549. Also, rGrA stimulated release of a neutrophil chemoattractant, interleukin-8, from the cells, via a mechanism involving microtubule disruption, probably resulting from reduction of cell adhesion to culture dishes. These findings suggest that GrA might be involved in the pathogenesis of certain lung diseases characterized by loss of alveolar wall structures, neutrophil accumulation, and chronic inflammation.
Yoshikawa
Yumiko
Y
Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Hirayasu
Hirofumi
H
Tsuzuki
Satoshi
S
Fushiki
Tohru
T
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned / Q000737:chemistry; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D003603:Cytotoxins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism*; D053804:Granzymes / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D003603:Cytotoxins; D016209:Interleukin-8; D011994:Recombinant Proteins; D053804:Granzymes