title: Comparative proteomic analysis of human placenta derived from assisted reproductive technology.
abstract: The aim of this study was to use proteomics-based approach to examine differences in protein expression in placenta derived from assisted reproductive technology (ART) and normal pregnancy. Using 2-DE we found that, compared with the control group, 12 spots in standard in vitro fertilization group and 18 spots in intracytoplasmic sperm injection group were identified as significantly differentially expressed proteins. Among them, six spots were differentially expressed in both standard IVF and ICSI groups with the same change tendency. Totally, 20 proteins were successfully identified by MALDI TOF/TOF MS, including proteins involved in the membrane traffic, metabolism, nucleic acid processing, stress response and cytoskeleton. Notably, five proteins detected to be differentially expressed in both ART groups were identified as annexin A3, hnRNP C1/C2, alpha-SNAP, FTL and ATP5A. Some of the proteins were confirmed by Western blot and immunohistochemistry analysis. Our study allowed for the initial identification of these proteins related to various functions in placentation with significantly altered abundance in ART groups. The present results reveal that abnormal protein profiles are involved in ART placenta and these differentially expressed proteins may be valuable for the evaluation of potential association between ART treatment and offspring outcome.
authors.affiliation: Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Zhang
authors.forename: Chun
authors.initials: C
authors.lastname: Feng
authors.forename: Yan-Ting
authors.initials: YT
authors.lastname: Wu
authors.forename: Ai-Xia
authors.initials: AX
authors.lastname: Liu
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Sheng
authors.forename: Jie
authors.initials: J
authors.lastname: Cai
authors.forename: He-Feng
authors.initials: HF
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D017318:Annexin A3 / Q000096:biosynthesis; D001052:Apoferritins; D015536:Down-Regulation; D005260:Female; D005293:Ferritins / Q000096:biosynthesis; D005307:Fertilization in Vitro; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C / Q000096:biosynthesis; D006801:Humans; D025261:Mitochondrial Proton-Translocating ATPases / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010086:Oxidative Phosphorylation Coupling Factors / Q000096:biosynthesis; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins / Q000096:biosynthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020554:Sperm Injections, Intracytoplasmic
chemical_list: C507433:FTL protein, human; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C; D010086:Oxidative Phosphorylation Coupling Factors; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D005293:Ferritins; D001052:Apoferritins; D017318:Annexin A3; C000628217:F(6) ATPase; D025261:Mitochondrial Proton-Translocating ATPases