Determination of trace alpha-fetoprotein variant by affinity adsorption solid substrate-room temperature phosphorimetry.
The 3.5-generation dendrimers (3.5G-D)-porphyrin (P) dual luminescent molecule (3.5G-D-P) was used to label concanavalin agglutinin (Con A); the product of the reaction is 3.5G-D-P-Con A. A new method for the determination of trace AFP-V by affinity adsorption solid substrate-room temperature phosphorimetry (AA-SS-RTP) was established, based on the room temperature phosphorescence (RTP) property of the product on polyamide membrane (PAM) substrate and the specific affinity adsorption (AA) reaction between 3.5G-D-P-Con A and alpha-fetoprotein variant (AFP-V), which caused the RTP of the system to be sharply enhanced, the DeltaIp was linearly correlated to the content of AFP-V. The sensitivity of the method was obviously high. It could accurately detect the content of AFP-V in serum. The results were tallied well with those obtained by the ELISA method.
Liu
Jia-Ming
JM
Department of Chemistry, Zhangzhou Normal College, Zhangzhou 363000, People's Republic of China. zzsyliujiaming@163.com
Liu
Zhen-Bo
ZB
Li
Xue-Lin
XL
Li
Zhi-Ming
ZM
Huang
Xiao-Mei
XM
Hong
Feng-Shan
FS
Lin
Wei-Nv
WN
Chen
Fang
F
D000327:Adsorption; D003890:Desiccation; D006801:Humans; D007477:Ions / Q000737:chemistry; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry; D013057:Spectrum Analysis / Q000379:methods*; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000737:chemistry
D007477:Ions; D020033:Protein Isoforms; D000509:alpha-Fetoproteins