Flow cytometric analysis of cytokine responses in stimulated whole blood: simultaneous quantitation of TNF-alpha-secreting cells and soluble cytokines.
This unit describes technical protocols aimed at the ex vivo or in vitro evaluation of the functional status of the immune system through the simultaneous identification and enumeration of cytokine-secreting cells and quantitation of the soluble cytokines produced by these cells.
Rodríguez-Caballero
Arancha
A
Universidad de Salamanca, Salamanca, Spain.
García-Montero
Andrés
A
Universidad de Salamanca, Salamanca, Spain.
Bueno
Clara
C
Universidad de Salamanca, Salamanca, Spain.
Orfao
Alberto
A
Universidad de Salamanca, Salamanca, Spain.
D035843:Biomedical Research / Q000379:methods; D001773:Blood Cells; D016207:Cytokines / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007107:Immune System; D007962:Leukocytes / Q000378:metabolism*; D008954:Models, Biological; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha