Development and validation of a fast quantitative method for plasma dimethylarginines analysis using liquid chromatography-tandem mass spectrometry.
OBJECTIVE
The aim of this work was to implement a fast, accurate and simple method to quantify plasma ADMA and SDMA, in a run time suitable for routine analysis.


METHODS
We developed and validated a hydrophilic interaction chromatographic method coupled to tandem mass spectrometry (HILIC-MS/MS) for separation and simultaneous quantification of Arginine (Arg) and its dimethylarginines, ADMA and SDMA, with a short run time (less than 5 min) using a small volume of human plasma (0.02 mL).


RESULTS
Correlation coefficients (r) of the calibration curves ranged from 0.9926 to 0.9984. Within-day and between-day imprecision (CV%) and inaccuracy (%), carry-over and recovery were also evaluated for validation. Preliminary data of Arg, ADMA and SDMA from 30 apparently healthy subjects and type 2 diabetic patients (n=33) with and without kidney dysfunction were calculated and some statistical differences occurred among them (p<0.05).


CONCLUSIONS
Data from calibration curves and quality controls reveal that the method is accurate and precise. Healthy subjects and diabetic patients' values are in agreement with those reported in other studies.
D'Apolito
Oceania
O
Clinical Biochemistry, Department of Biomedical Sciences, Faculty of Medicine-University of Foggia, Viale L. Pinto, 1-71100 Foggia, Italy.
Paglia
Giuseppe
G
Tricarico
Filomena
F
Garofalo
Daniela
D
Pilotti
Alessandra
A
Lamacchia
Olga
O
Cignarelli
Mauro
M
Corso
Gaetano
G
D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D011786:Quality Control; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
C487735:dimethylarginine; C024917:symmetric dimethylarginine; C018524:N,N-dimethylarginine; D001120:Arginine