The ribosomal protein L10/QM-like protein is a component of the NIK-mediated antiviral signaling.
The NIK (NSP-interacting kinase)-mediated antiviral signaling pathway was identified as a virulence target of the begomovirus nuclear shuttle protein (NSP). Here, we further characterized this layer of plant innate defense by identifying the ribosomal protein L10 (rpL10), a QM-like protein, as a downstream effector of the antiviral signaling. Although both ribosomal proteins rpL10 and rpL18 were found to associate with NIK1 through yeast two-hybrid screening, the NIK receptors specifically phosphorylated rpL10 in vitro. Furthermore, loss of rpL10 function significantly increased susceptibility to begomovirus infection, recapitulating the phenotype of nik knockout lines. Our results genetically linked rpL10 to the NIK-mediated antiviral signaling.
Rocha
Carolina S
CS
Departamento de Bioquímica e Biologia Molecular, BIOAGRO, Universidade Federal de Viçosa, 36570.000 Viçosa, MG, Brazil.
Santos
Anésia A
AA
Machado
João Paulo B
JP
Fontes
Elizabeth P B
EP
D017360:Arabidopsis / Q000821:virology; D029681:Arabidopsis Proteins; D053839:Begomovirus / Q000276:immunology* / Q000502:physiology*; D010766:Phosphorylation; D010935:Plant Diseases / Q000276:immunology / Q000821:virology; D010940:Plant Proteins / Q000276:immunology / Q000378:metabolism*; D025941:Protein Interaction Mapping; D011494:Protein Kinases / Q000276:immunology* / Q000378:metabolism; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism*
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D012269:Ribosomal Proteins; D014764:Viral Proteins; D037521:Virulence Factors; C000630691:ribosomal protein L10, Arabidopsis; D011494:Protein Kinases