Micro RNA like inhibition of HIV-1 replication.
In this study, we investigated an RNA (R-Psi-sgRNA) that suppresses HIV-1 replication. This RNA is expressed by a plasmid vector (pR-Psi-sgRNA-ter) that was constructed accidentally. To examine if this effect is caused by RNA interference, R-Psi-sgRNA was synthesized in vitro and treated with the Dicer enzyme, an important RNase III enzyme for RNA interference. The RNA was cleaved into fragments of approximately 20 nucleotides. We then performed an HIV-1 p24 assay with the RNA fragments to evaluate their effect on HIV-1 replication. HIV-1 replication was suppressed.
Kato
Keijiro
K
Department of Life and Environmental Science, Chiba Institute of Technology, Narashino, Chiba 275-0016, Japan.
Habu
Yuichiro
Y
Takaku
Hiroshi
H
D005822:Genetic Vectors; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D034622:RNA Interference; D043244:Ribonuclease III / Q000378:metabolism; D014779:Virus Replication; D058727:RNA, Small Untranslated
D016655:HIV Core Protein p24; D035683:MicroRNAs; C514799:p24 protein, Human Immunodeficiency Virus Type 1; D043244:Ribonuclease III; D058727:RNA, Small Untranslated