[Effects of Porphyromonas gingivalis on inflammatory factor secreted by human umbilical vein endothelial cells].
OBJECTIVE
To investigate the effect of porphyromonas gingivalis (P.g) on interleukin-18 (IL-18) and CRP secretion in human umbilical vein endothelial cells (HUVECs).


METHODS
Anaeropack method was used for P.g cultivation and then HUVECs were co-cultured with P.g followed by assaying the amount of IL-18 and CRP with enzyme-linked immunosorbent assay (ELISA) kit. Linear regression and variance analysis were performed with SPSS11.0 software package.


RESULTS
The unstimulated HUVECs expressed small amounts of IL-18 and CRP, P.g dose-dependently increased the production of IL-18 and CRP in HUVECs. Elevated IL-18 and CRP were found at 4-hour, then continued to increase at 8-hour, 12-hour and 24-hour after co-cultivation; under the effect of same concentration, the quantity of CRP was significantly higher than that of IL-18 secreted by HUVECs. There was significant different, P<0.05.


CONCLUSIONS
It is concluded that P.g increased the production of IL-18 and CRP in HUVECs in a dose and time dependent manner, elevated IL-18 and CRP may be involved in the regulation of inflammatory both in periodontal disease and formation of atherosclerotic plaque in cardiovascular disease(CVD).
Wang
Xiao-yan
XY
School of Stomatology, The Fourth Military Medical University, Xi'an 710032, Shanxi province, China.
Wang
Jun-lian
JL
Ding
Ao
A
Sun
Yan
Y
Wang
Qin-tao
QT
D050197:Atherosclerosis; D002097:C-Reactive Protein / Q000378:metabolism; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation; D020382:Interleukin-18 / Q000378:metabolism; D010510:Periodontal Diseases; D016966:Porphyromonas gingivalis
D020382:Interleukin-18; D002097:C-Reactive Protein