SPR-imaging based assays on an oligonucleotide-array to analyze DNA lesions recognition and excision by repair proteins.
An original oligonucleotide-array, coupled with SPR-imaging detection, has been developed to study biological interactions between DNA base lesions and DNA repair enzymes. This bioanalytical tool constitutes an efficient screening platform to quantify DNA repair activities and to search for new DNA repair inhibitors.
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Laboratoire Lésions des Acides Nucléiques, SCIB/UMR E3 CEA-UJF, INAC, CEA-Grenoble, F-38054 Grenoble Cedex 9, France.
Fiche
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Cunin
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Buhot
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Fuchs
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Calemczuk
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Favier
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Livache
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Gasparutto
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D000080242:8-Hydroxy-2'-Deoxyguanosine; D004249:DNA Damage; D004260:DNA Repair; D043382:DNA-Formamidopyrimidine Glycosylase / Q000378:metabolism*; D003839:Deoxyadenosines / Q000737:chemistry; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020349:Surface Plasmon Resonance
D003839:Deoxyadenosines; D029968:Escherichia coli Proteins; C056752:8,5'-cyclo-2'-deoxyadenosine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D043382:DNA-Formamidopyrimidine Glycosylase; C468610:DNA-formamidopyrimidine glycosylase, E coli; D003849:Deoxyguanosine