Four different integrative recombination events involved in the mobilization of the gonococcal 5.2 kb beta-lactamase plasmid pSJ5.2 in Escherichia coli.
We identified and characterized four different recombination mechanisms involved in the cointegrative transfer of the Neisseria gonorrhoeae beta-lactamase plasmid pSJ5.2 by the gonococcal 41 kb tet(M) and the Gram negative self-transmissible plasmids N3 and R64 drd-33 using an Escherichia colirecA-background. Mobilization of pSJ5.2 by the tet(M) plasmid occurred by cointegration through a replicative transposition of two IS1 elements inserted upstream from the beta-lactamase gene of pSJ5.2 and creating a IS1::beta-lactamase hybrid promoter. Two types of recombinational events occurred within the 1.8 kb BamH1-HindIII fragment of pSJ5.2 with the N3 and R64 plasmids. A non-homologous recombination was found at coordinates 1817 and 2849 of pSJ5.2 with sequences from R64. A non-homologous recombination combined with an IS26-mediated one-ended transposition was found at coordinates 1817 and 3010 of pSJ5.2 with N3. In both recombinational events, a deletion of over 1 kb of pSJ5.2 occurred. The fourth recombination event was detected in the 1.0 kb BamH1-HindIII fragment of pSJ5.2 by homologous recombination between DNA from the truncated Tn3 resolvase gene of pSJ5.2 and the resolvase sequences from R64 and N3.
Scharbaai-Vázquez
Ramón
R
Department of Microbiology, San Juan Bautista School of Medicine, P.O. Box 4968, Caguas, PR 00726-4968, USA.
González-Caraballo
Ana L
AL
Torres-Bauzá
Luis J
LJ
D000668:Ampicillin Resistance; D020029:Base Pairing / Q000235:genetics*; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D015247:Deoxyribonuclease HindIII; D004926:Escherichia coli / Q000235:genetics*; D018628:Gene Dosage; D016254:Mutagenesis, Insertional; D009344:Neisseria gonorrhoeae / Q000201:enzymology* / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D015247:Deoxyribonuclease HindIII; D001618:beta-Lactamases
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