title: Protective effects of epigallocatechin gallate after UV irradiation of cultured human lens epithelial cells.
abstract: OBJECTIVE
  To evaluate the protective effects of epigallocatechin gallate (EGCG) against UV irradiation of cultured human lens epithelial cells.
  
  
  METHODS
  We irradiated cultured human lens epithelial cells with a 30-second pulse from a UV lamp with an irradiance of 0.6 mW/cm2. Five minutes and 1 hour after UV irradiation, we administered 0, 5, 10, 15, 25, 50, or 100 uM EGCG. The cell number was measured with a microscopic counting chamber and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.
  
  
  RESULTS
  Compared to untreated cells, the total number of cultured human lens epithelial cells was markedly higher after UV irradiation. In a dose-dependent manner, viability was also higher in EGCG-treated cells.
  
  
  CONCLUSIONS
  EGCG increased the cell count and cell viability after UV irradiation of cultured human lens epithelial cells, indicating that EGCG can protect lens epithelium against UV damage.
authors.affiliation: Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Korea.
authors.forename: Jun
authors.initials: J
authors.lastname: Heo
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Lee
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Koh
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000528:radiation effects; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000528:radiation effects*; D011832:Radiation Injuries / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D004396:Coloring Agents; D011837:Radiation-Protective Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D002392:Catechin; C045651:epigallocatechin gallate; C022616:thiazolyl blue
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