Nitric oxide interacts with salicylate to regulate biphasic ethylene production during the hypersensitive response.
C(2)H(4) is associated with plant defense, but its role during the hypersensitive response (HR) remains largely uncharacterized. C(2)H(4) production in tobacco (Nicotiana tabacum) following inoculation with HR-eliciting Pseudomonas syringae pathovars measured by laser photoacoustic detection was biphasic. A first transient rise (C(2)H(4)-I) occurred 1 to 4 h following inoculation with HR-eliciting, disease-forming, and nonpathogenic strains and also with flagellin (flg22). A second (avirulence-dependent) rise, at approximately 6 h (C(2)H(4)-II), was only seen with HR-eliciting strains. Tobacco leaves treated with the C(2)H(4) biosynthesis inhibitor, aminoethoxyvinylglycine, suggested that C(2)H(4) influenced the kinetics of a HR. Challenging salicylate hydroxylase-expressing tobacco lines and tissues exhibiting systemic acquired resistance suggested that C(2)H(4) production was influenced by salicylic acid (SA). Disrupted expression of a C(2)H(4) biosynthesis gene in salicylate hydroxylase tobacco plants implicated transcriptional control as a mechanism through which SA regulates C(2)H(4) production. Treating leaves to increase oxidative stress or injecting with SA initiated monophasic C(2)H(4) generation, but the nitric oxide (NO) donor sodium nitroprusside initiated biphasic rises. To test whether NO influenced biphasic C(2)H(4) production during the HR, the NO synthase inhibitor N(G)-nitro-L-arginine methyl ester was coinoculated with the avirulent strain of P. syringae pv phaseolicola into tobacco leaves. The first transient C(2)H(4) rise appeared to be unaffected by N(G)-nitro-L-arginine methyl ester, but the second rise was reduced. These data suggest that NO and SA are required to generate the biphasic pattern of C(2)H(4) production during the HR and may influence the kinetics of HR formation.
Mur
Luis A J
LA
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Wales SY23 3DA, United Kingdom. lum@aber.ac.uk
Laarhoven
Lucas J J
LJ
Harren
Frans J M
FJ
Hall
Michael A
MA
Smith
Aileen R
AR
D005030:Ethylenes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D018384:Oxidative Stress; D044224:Pseudomonas syringae / Q000502:physiology; D012459:Salicylates / Q000378:metabolism*; D014026:Nicotiana / Q000201:enzymology / Q000378:metabolism* / Q000382:microbiology
D005030:Ethylenes; D020030:Nitric Oxide Donors; D012459:Salicylates; D009599:Nitroprusside; D009569:Nitric Oxide; C036216:ethylene; D006899:Mixed Function Oxygenases; C022619:salicylate 1-monooxygenase; C088137:aminoethoxyvinylglycine; D005998:Glycine
Plant J. 2006 Sep;47(6):907-16
16961732
Plant J. 2000 Dec;24(5):667-77
11123805
Plant J. 2003 Nov;36(3):342-52
14617091
Trends Plant Sci. 2003 Jun;8(6):245-7
12818655
Appl Environ Microbiol. 2002 Nov;68(11):5342-50
12406723
J Exp Bot. 2003 Nov;54(392):2449-56
14565949
Nature. 1998 Aug 6;394(6693):585-8
9707120
Plant Cell Environ. 2007 Jan;30(1):39-52
17177875
J Bacteriol. 1991 Jan;173(2):575-86
1846144
Plant Physiol. 2006 Oct;142(2):595-608
16935990
Adv Microb Physiol. 1993;35:275-306
8310882
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5202-6
16593860
Phytopathology. 2001 May;91(5):511-8
18943596
Plant J. 2002 Nov;32(4):447-56
12445117
Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13454-9
11606758
Mol Plant Microbe Interact. 1992 Sep-Oct;5(5):372-8
1472714
Cell. 2005 Jun 3;121(5):749-59
15935761
Plant Cell. 1998 Dec;10(12):2103-13
9836748
Trends Plant Sci. 2006 Apr;11(4):184-91
16531096
J Exp Bot. 2006;57(3):489-505
16377733
Annu Rev Phytopathol. 2004;42:385-414
15283671
Plant Physiol. 1999 Dec;121(4):1093-102
10594097
Annu Rev Plant Biol. 2003;54:23-61
14502984
Plant Cell. 1997 Feb;9(2):261-70
9061956
Cell. 1994 Nov 18;79(4):583-93
7954825
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10328-33
9707647
Plant Cell. 1994 Jul;6(7):927-33
8069104
Eur J Biochem. 2003 Jun;270(12):2593-604
12787025
Plant Physiol. 1999 Mar;119(3):935-50
10069832
Plant Cell. 1995 Oct;7(10):1529-1536
12242354
Plant Physiol. 2002 Dec;130(4):1918-26
12481074
Plant J. 2000 Sep;23(5):609-21
10972887
Appl Opt. 1982 Nov 15;21(22):4092-100
20401015
Plant J. 2000 Aug;23(4):441-50
10972870
Plant J. 1998 Oct;16(2):235-45
22507138
Mol Plant Microbe Interact. 1995 Jan-Feb;8(1):177-80
7772800
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15811-6
15498873
Planta. 2002 Feb;214(4):537-45
11925037
Proc Natl Acad Sci U S A. 1998 May 12;95(10):5818-23
9576968
Mol Microbiol. 1998 Aug;29(4):1101-12
9767577
Plant Cell. 1998 Mar;10(3):371-82
9501111
Plant Physiol. 2000 May;123(1):81-92
10806227
Appl Environ Microbiol. 1997 Jan;63(1):156-61
16535480
Plant Physiol. 1986 Jul;81(3):929-30
16664928
Plant Cell. 1992 Nov;4(11):1359-69
1477552
Mol Gen Genet. 1997 Oct;256(3):211-22
9393445
J Exp Bot. 2006;57(3):507-16
16396997
Plant J. 2003 Jan;33(2):245-57
12535339
Mol Plant Microbe Interact. 2000 Apr;13(4):430-8
10755306
Plant Physiol. 1992 Sep;100(1):219-24
16652950
Nature. 2004 Apr 15;428(6984):764-7
15085136
Mol Plant Pathol. 2002 Sep 1;3(5):371-90
20569344
Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2275-80
11854524
Annu Rev Phytopathol. 2006;44:135-62
16602946
Nat Immunol. 2005 Oct;6(10):973-9
16177805
Plant J. 2002 Jan;29(1):23-32
12060224
Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:251-275
15012264
Plant Physiol. 1991 Nov;97(3):1181-6
16668506
Annu Rev Cell Dev Biol. 2000;16:1-18
11031228
Plant Physiol. 2005 Jul;138(3):1247-58
16009999