Oxidative metabolism.
Peroxide production is detected using DCFH-DA, superoxide anion by hydroethidine; the methods can be combined for simultaneous detection. A support protocol describes calibration of the flow cytometer to permit calculation of the actual H2O2 production per cell. These protocols are applicable to many different cell types, but provide detailed information on using neutrophils, highly reactive cells that produce vast quantities of ROS.
Robinson
J P
JP
Purdue University Cytometry Laboratories, West Lafayette, Indiana, USA.
D000818:Animals; D000838:Anions; D002138:Calibration; D002469:Cell Separation; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D009504:Neutrophils / Q000166:cytology*; D010100:Oxygen / Q000737:chemistry*; D010545:Peroxides / Q000737:chemistry; D017382:Reactive Oxygen Species; D013481:Superoxides / Q000737:chemistry
D000838:Anions; D010545:Peroxides; D017382:Reactive Oxygen Species; D013481:Superoxides; D006861:Hydrogen Peroxide; D010100:Oxygen