title: Myosin II has distinct functions in PNS and CNS myelin sheath formation.
abstract: The myelin sheath forms by the spiral wrapping of a glial membrane around the axon. The mechanisms responsible for this process are unknown but are likely to involve coordinated changes in the glial cell cytoskeleton. We have found that inhibition of myosin II, a key regulator of actin cytoskeleton dynamics, has remarkably opposite effects on myelin formation by Schwann cells (SC) and oligodendrocytes (OL). Myosin II is necessary for initial interactions between SC and axons, and its inhibition or down-regulation impairs their ability to segregate axons and elongate along them, preventing the formation of a 1:1 relationship, which is critical for peripheral nervous system myelination. In contrast, OL branching, differentiation, and myelin formation are potentiated by inhibition of myosin II. Thus, by controlling the spatial and localized activation of actin polymerization, myosin II regulates SC polarization and OL branching, and by extension their ability to form myelin. Our data indicate that the mechanisms regulating myelination in the peripheral and central nervous systems are distinct.
authors.affiliation: Department of Biological Sciences, Hunter College, City University of New York, New York, NY 10065, USA.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.forename: Ambika
authors.initials: A
authors.lastname: Tewari
authors.forename: Steven
authors.initials: S
authors.lastname: Einheber
authors.forename: James L
authors.initials: JL
authors.lastname: Salzer
authors.forename: Carmen V
authors.initials: CV
authors.lastname: Melendez-Vasquez
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D015415:Biomarkers / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002490:Central Nervous System / Q000378:metabolism*; D018920:Coculture Techniques; D003599:Cytoskeleton / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology; D006576:Heterocyclic Compounds, 4 or More Rings / Q000378:metabolism; D004676:Myelin Basic Protein / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D024462:Myosin Type II / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D017933:Peripheral Nervous System / Q000378:metabolism*; D034622:RNA Interference; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D000199:Actins; D015415:Biomarkers; D015820:Cadherins; D006576:Heterocyclic Compounds, 4 or More Rings; D004676:Myelin Basic Protein; C472645:blebbistatin; D024462:Myosin Type II
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