Fluorescent ligand as a molecular probe for the RNA structure.
In this report, we show the recognition of RNA secondary structure by exploiting a fluorescent 2,7-disubstituted 9H-xanthen-9-one derivative (2). The xanthone derivertive (2) especially bound to RNA and became non-fluorescent. Upon mixing with RRE model RNA, U-bulge RNA, or hairpin RNA, the fluorescence intensity of 2 was decreased in different intensity. The distinct fluorescent increase of 2 to each RNA seems to a good probe for The RNA structures.
Umemoto
Shiori
S
The institute of Scientific and Industrial Reserch, Osaka University, Mihogaoka 8-1, Ibaraki 567-0047, Japan.
Zhang
Jinhua
J
Dohno
Chikara
C
Nakatani
Kazuhiko
K
D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008024:Ligands; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D044004:Xanthones / Q000138:chemical synthesis / Q000737:chemistry*
C541617:2,7-bisaminoethoxyxanthen-9-one; D005456:Fluorescent Dyes; D008024:Ligands; D044004:Xanthones; D012313:RNA