title: Altered expression of UVB-induced cytokines in human papillomavirus-immortalized epithelial cells.
abstract: Keratinocytes can be induced to produce cytokines by exogenous stimuli, such as UVB, and dysregulation of this production has been described in various skin diseases, including cancer. In this study, we compared the effect of UVB on the secretion of several cytokines involved in inflammation by human keratinocytes immortalized or not with human papillomavirus (HPV)16 or HPV38 at the mRNA and protein levels. We show that expression of the HPV E6/E7 oncoproteins influences not only the basal cytokine secretion profile of keratinocytes, but also its modulation upon UVB irradiation. In particular, UVB upregulates interleukin (IL)-6, IL-8 and transforming growth factor (TGF)-beta in HPV-immortalized cells to a higher extent than in control keratinocytes. Moreover, expression of other pro-inflammatory molecules such as S100A8/9 and interferon (IFN)-kappa was downregulated in HPV-immortalized cells. These data support the functional similarity between HPV16 and 38, and suggest an active role of these viruses in modulation of the inflammatory process.
authors.affiliation: Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Dell'Oste
authors.affiliation: Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Azzimonti
authors.affiliation: NoToPharm s.r.l., Bioindustry Park del Canavese, Via Ribes 5, 10010 Colleretto Giacosa (TO), Italy.|Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Mondini
authors.affiliation: Department of Public Health and Microbiology, Medical School of Torino, Via Santena 9, 10126 Torino, Italy.|Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: De Andrea
authors.affiliation: Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Borgogna
authors.affiliation: Department of Medical Sciences, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Mesturini
authors.affiliation: International Agency for Research on Cancer-World Health Organization, 150 Cours Albert Thomas, 69372 Lyon, France.
authors.forename: Rosita
authors.initials: R
authors.lastname: Accardi
authors.affiliation: International Agency for Research on Cancer-World Health Organization, 150 Cours Albert Thomas, 69372 Lyon, France.
authors.forename: Massimo
authors.initials: M
authors.lastname: Tommasino
authors.affiliation: NoToPharm s.r.l., Bioindustry Park del Canavese, Via Ribes 5, 10010 Colleretto Giacosa (TO), Italy.|Department of Public Health and Microbiology, Medical School of Torino, Via Santena 9, 10126 Torino, Italy.
authors.forename: Santo
authors.initials: S
authors.lastname: Landolfo
authors.affiliation: International Agency for Research on Cancer-World Health Organization, 150 Cours Albert Thomas, 69372 Lyon, France.
authors.forename: Umberto
authors.initials: U
authors.lastname: Dianzani
authors.affiliation: NoToPharm s.r.l., Bioindustry Park del Canavese, Via Ribes 5, 10010 Colleretto Giacosa (TO), Italy.|Department of Clinical and Experimental Medicine, Medical School of Novara, Via Solaroli 17, 28100 Novara, Italy.
authors.forename: Marisa
authors.initials: M
authors.lastname: Gariglio
mesh_terms: D052686:Betapapillomavirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D016207:Cytokines / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D005786:Gene Expression Regulation; D052162:Human papillomavirus 16 / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D007249:Inflammation; D015603:Keratinocytes / Q000276:immunology / Q000821:virology; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D016207:Cytokines; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16