title: Assessment of paraoxonase activities in patients with knee osteoarthritis.
abstract: The objective of this study was to investigate serum paraoxonase and arylesterase activities, and lipid hydroperoxide (LOOH) and total thiol (total free sulfhydryl groups, -SH) levels along with lipid parameters in patients with knee osteoarthritis. Thirty-six patients with knee osteoarthritis and 30 healthy individuals were enrolled in the study. Serum paraoxonase and arylesterase activities were measured spectrophotometrically. LOOH levels were measured by ferrous oxidation with xylenol orange assay (FOX-2). Serum high-density lipoprotein-cholesterol (HDL-C), -SH levels, paraoxonase and arylesterase activities were significantly lower in the patient group than those in the controls (P < 0.05, for all), while LOOH and low-density lipoprotein (LDL) levels were significantly higher. In conclusion, paraoxonase and arylesterase activities were decreased significantly in patients with knee osteoarthritis. Lower serum paraoxonase-1 activity and lower level of HDL-C seem to be related to increased oxidative stress and inflammatory condition in these patients. It is known that paraoxonases reduce oxidative stress in serum and tissues thereby protecting against cardiovascular disease, particularly atherosclerosis. Thus, decreased paraoxonase and arylesterase activities play a role in the pathogenesis of atherosclerosis through increased susceptibility to lipid peroxidation in patients with osteoarthritis.
authors.affiliation: Department of Physical Medicine and Rehabilitation, Harran University Research Hospital, Sanliurfa, Turkey. neslihansoran@yahoo.com
authors.forename: Neslihan
authors.initials: N
authors.lastname: Soran
authors.forename: Ozlem
authors.initials: O
authors.lastname: Altindag
authors.forename: Hale
authors.initials: H
authors.lastname: Cakir
authors.forename: Hakim
authors.initials: H
authors.lastname: Celik
authors.forename: Ahmet
authors.initials: A
authors.lastname: Demirkol
authors.forename: Nurten
authors.initials: N
authors.lastname: Aksoy
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis; D043303:Aryldialkylphosphatase / Q000097:blood*; D050197:Atherosclerosis / Q000209:etiology; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D005260:Female; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D020370:Osteoarthritis, Knee / Q000150:complications / Q000201:enzymology*; D018384:Oxidative Stress; D013438:Sulfhydryl Compounds / Q000097:blood
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human