title: Solubility of lysozyme in the presence of aqueous chloride salts: common-ion effect and its role on solubility and crystal thermodynamics.
abstract: Understanding protein solubility is important for a rational design of the conditions of protein crystallization. We report measurements of lysozyme solubility in aqueous solutions as a function of NaCl, KCl, and NH4Cl concentrations at 25 degrees C and pH 4.5. Our solubility results are directly compared to preferential-interaction coefficients of these ternary solutions determined in the same experimental conditions by ternary diffusion. This comparison has provided new important insight on the dependence of protein solubility on salt concentration. We remark that the dependence of the preferential-interaction coefficient as a function of salt concentration is substantially shaped by the common-ion effect. This effect plays a crucial role also on the observed behavior of lysozyme solubility. We find that the dependence of solubility on salt type and concentration strongly correlates with the corresponding dependence of the preferential-interaction coefficient. Examination of both preferential-interaction coefficients and second virial coefficients has allowed us to demonstrate that the solubility dependence on salt concentration is substantially affected by the corresponding change of protein chemical potential in the crystalline phase. We propose a simple model for the crystalline phase based on salt partitioning between solution and the hydrated protein crystal. A novel solubility equation is reported that quantitatively explains the observed experimental dependence of protein solubility on salt concentration.
authors.affiliation: Department of Chemistry, Texas Christian University, Fort Worth, Texas 76129, USA.
authors.forename: Onofrio
authors.initials: O
authors.lastname: Annunziata
authors.forename: Andrew
authors.initials: A
authors.lastname: Payne
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002645:Chickens; D002712:Chlorides / Q000737:chemistry*; D003460:Crystallization; D007477:Ions / Q000737:chemistry; D008954:Models, Biological; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D012492:Salts / Q000737:chemistry*; D012995:Solubility; D013816:Thermodynamics
chemical_list: D002712:Chlorides; D007477:Ions; D012492:Salts; D009113:Muramidase