title: Microarray based analysis of temperature and oxidative stress induced messenger RNA in Schistosoma mansoni.
abstract: The body's defense against schistosome infection can take many forms. For example, upon developing acute schistosomiasis, patients often have fever coinciding with larval maturation, migration and early oviposition. As the infection becomes established, the parasite comes under oxidative stress generated by the host immune system. The most common treatment for schistosomiasis is the anti-helminthic drug praziquantel. Its effectiveness, however, is limited due to its inability to kill schistosomes 2-4 weeks post-infection. Clearly there is a need for new anti-schistosomal drugs. We hypothesize that gene products expressed as part of a protective response against heat and/or oxidative stress are potential therapeutic targets for future drug development. Using a 12,166 element oligonucleotide microarray to characterize Schistosoma mansoni genes induced by heat and oxidative stress we found that 1878 S. mansoni elements were significantly induced by heat stress. These included previously reported heat-shock genes expressing homologs of HSP40, HSP70 and HSP86. One thousand and one elements were induced by oxidative stress including those expressing homologs of superoxide dismutase, glutathione peroxidase and aldehyde dehydrogenase. Seventy-two elements were common to both stressors and could potentially be exploited in the development of novel anti-schistosomal therapeutics.
authors.affiliation: Department of Biology, 216 Castetter Hall, University of New Mexico, Albuquerque, NM 87131, USA.
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Aragon
authors.forename: Reza A
authors.initials: RA
authors.lastname: Imani
authors.forename: Vint R
authors.initials: VR
authors.lastname: Blackburn
authors.forename: Charles
authors.initials: C
authors.lastname: Cunningham
mesh_terms: D000818:Animals; D017238:Genes, Helminth; D046228:Microarray Analysis; D018384:Oxidative Stress / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D012550:Schistosoma mansoni / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger
references.citation: Nucleic Acids Res. 2003 Nov 15;31(22):e142
references.pmid: 14602935
references.citation: Biochem J. 2004 Apr 15;379(Pt 2):291-9
references.pmid: 14711373
references.citation: Exp Parasitol. 1986 Jun;61(3):294-303
references.pmid: 3086114
references.citation: Gene. 1999 Aug 20;236(2):197-208
references.pmid: 10452940
references.citation: Trends Biochem Sci. 2005 May;30(5):235-9
references.pmid: 15896740
references.citation: Protein Sci. 2001 Jul;10(7):1353-62
references.pmid: 11420437
references.citation: Drug Metab Rev. 2004 May;36(2):279-99
references.pmid: 15237855
references.citation: J Neurochem. 2001 May;77(4):1010-7
references.pmid: 11359866
references.citation: Int J Parasitol. 2002 Sep;32(10):1285-92
references.pmid: 12204228
references.citation: Mol Biochem Parasitol. 2006 Apr;146(2):180-91
references.pmid: 16427147
references.citation: Antioxid Redox Signal. 2005 Jul-Aug;7(7-8):1040-52
references.pmid: 15998259
references.citation: J Biol Chem. 2007 Sep 7;282(36):26140-9
references.pmid: 17631501
references.citation: Mol Biochem Parasitol. 2001 Jun;115(1):87-99
references.pmid: 11377743
references.citation: J Biol Chem. 1993 Oct 5;268(28):21218-24
references.pmid: 8407958
references.citation: Neuroscience. 2007 Apr 14;145(4):1201-12
references.pmid: 17101234
references.citation: Trop Med Int Health. 2001 Nov;6(11):864-73
references.pmid: 11703840
references.citation: J Biol Chem. 2004 Sep 3;279(36):37407-14
references.pmid: 15231836
references.citation: Bull World Health Organ. 2001;79(8):695-703
references.pmid: 11545325
references.citation: Free Radic Biol Med. 2002 Jul 1;33(1):15-28
references.pmid: 12086678
references.citation: Mol Biochem Parasitol. 2005 May;141(1):1-13
references.pmid: 15811522
references.citation: Am J Trop Med Hyg. 2005 May;72(5):540-548
references.pmid: 15891127
references.citation: Mol Cell Biol. 1997 Oct;17(10):6029-39
references.pmid: 9315662
references.citation: Genome Biol. 2006;7(2):R9
references.pmid: 16507144
references.citation: J Bacteriol. 2000 Jan;182(1):23-9
references.pmid: 10613858
references.citation: Parasitology. 2000;121 Suppl:S23-38
references.pmid: 11386688
references.citation: Drugs. 2003;63(10):933-51
references.pmid: 12699398
references.citation: Eur J Biochem. 1993 Mar 1;212(2):589-96
references.pmid: 8444195
references.citation: Biomed Pharmacother. 2003 May-Jun;57(3-4):145-55
references.pmid: 12818476
references.citation: Int J Parasitol. 2004 Mar 29;34(4):527-33
references.pmid: 15013742
references.citation: Nat Genet. 2003 Oct;35(2):148-57
references.pmid: 12973350
references.citation: J Bacteriol. 1995 Jun;177(11):3344-6
references.pmid: 7768839
references.citation: PLoS Med. 2007 Jun;4(6):e206
references.pmid: 17579510
references.citation: Antioxid Redox Signal. 2007 Jan;9(1):25-47
references.pmid: 17115886
references.citation: Eur J Biochem. 1986 Dec 15;161(3):525-31
references.pmid: 3539592
references.citation: FEBS Lett. 2002 Dec 4;532(1-2):147-52
references.pmid: 12459480
references.citation: Endocrinology. 2007 May;148(5):2148-56
references.pmid: 17289849
references.citation: Mem Inst Oswaldo Cruz. 2006 Jun;101(4):365-72
references.pmid: 16951805
references.citation: Exp Parasitol. 2006 Nov;114(3):160-72
references.pmid: 16631746
references.citation: Mol Biochem Parasitol. 2001 May;114(2):129-42
references.pmid: 11378193
references.citation: Nat Med. 2008 Apr;14(4):407-12
references.pmid: 18345010
references.citation: Mol Genet Genomics. 2006 Jan;275(1):89-96
references.pmid: 16292667
references.citation: Genes Dev. 2002 Mar 15;16(6):673-5
references.pmid: 11963920
references.citation: DNA Res. 2005;12(3):211-4
references.pmid: 16303752
references.citation: Nucleic Acids Res. 1999 May 1;27(9):2022-8
references.pmid: 10198436
references.citation: Free Radic Biol Med. 1997;22(3):497-507
references.pmid: 8981042
references.citation: Mol Biochem Parasitol. 1989 Aug;36(1):19-28
references.pmid: 2509907
references.citation: Mol Biochem Parasitol. 1986 Nov;21(2):179-88
references.pmid: 3097539
references.citation: Nucleic Acids Res. 1988 Feb 25;16(4):1333-48
references.pmid: 3279391
references.citation: Am J Physiol Endocrinol Metab. 2006 Nov;291(5):E867-77
references.pmid: 16822958
references.citation: Z Parasitenkd. 1977 Jul 21;52(2):129-50
references.pmid: 410178
references.citation: Mol Cell Probes. 2006 Oct;20(5):280-9
references.pmid: 16647836
references.citation: Int J Parasitol. 2004 Jul;34(8):979-87
references.pmid: 15217737
references.citation: Genes Dev. 1997 Sep 1;11(17):2272-90
references.pmid: 9303542
references.citation: J Biol Chem. 2006 Jun 23;281(25):17001-17010
references.pmid: 16606626
references.citation: Bioinformatics. 2005 Sep 15;21(18):3674-6
references.pmid: 16081474
references.citation: BMC Genomics. 2004 Mar 09;5(1):20
references.pmid: 15113400
references.citation: J Mol Biol. 2003 Aug 1;331(1):123-38
references.pmid: 12875840
references.citation: Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16849-54
references.pmid: 15548604
references.citation: Acta Trop. 2000 Oct 23;77(1):41-51
references.pmid: 10996119
references.citation: Vaccine. 2003 Dec 8;22(1):130-6
references.pmid: 14604580
references.citation: Circ Res. 2000 Sep 1;87(5):385-91
references.pmid: 10969036
references.citation: J Infect. 2006 May;52(5):339-45
references.pmid: 16169593
references.citation: Am J Trop Med Hyg. 1956 Sep;5(5):831-40
references.pmid: 13362750
references.citation: Proc Natl Acad Sci U S A. 1996 May 14;93(10):5116-21
references.pmid: 8643537
references.citation: Mil Med. 2007 Feb;172(2):144-6
references.pmid: 17357767
references.citation: Mol Cell. 2005 Jan 21;17(2):301-11
references.pmid: 15664198
references.citation: Nat Rev Mol Cell Biol. 2004 Oct;5(10):781-91
references.pmid: 15459659
references.citation: Int J Parasitol. 2006 Sep;36(10-11):1113-22
references.pmid: 16839558
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Trans R Soc Trop Med Hyg. 1984;78(3):385-8
references.pmid: 6464135
references.citation: Mol Biol Cell. 2000 Dec;11(12):4347-58
references.pmid: 11102528
references.citation: Trans R Soc Trop Med Hyg. 2002 Sep-Oct;96(5):465-9
references.pmid: 12474468
references.citation: Biochem Biophys Res Commun. 2000 Apr 21;270(3):1036-40
references.pmid: 10772946
references.citation: Genomics. 1998 Dec 1;54(2):331-7
references.pmid: 9828137
references.citation: Mol Cell Biol. 2003 Jul;23(13):4598-610
references.pmid: 12808100
references.citation: Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15223-8
references.pmid: 9860950
references.citation: Am J Physiol Renal Physiol. 2007 Jul;293(1):F306-15
references.pmid: 17459951
references.citation: Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):7872-5
references.pmid: 9653107
references.citation: Nucleic Acids Res. 1992 Dec 25;20(24):6605-11
references.pmid: 1480481
references.citation: J Biol Chem. 1998 Nov 6;273(45):29857-63
references.pmid: 9792702
references.citation: Eur J Biochem. 1996 Jun 15;238(3):838-44
references.pmid: 8706688
references.citation: Annu Rev Physiol. 1999;61:243-82
references.pmid: 10099689
references.citation: Cell Mol Life Sci. 2006 Apr;63(7-8):919-29
references.pmid: 16570121