title: [Lymphocyte subsets and preeclampsia].
abstract: BACKGROUND
  Preeclampsia origin has no conclusive explanation. As part of its etiology it has been proposed immunologic disorders. This work explores several lymphocytes subsets and postulates possible mechanisms involved in a lost of immune tolerance in this entity.
  
  
  OBJECTIVE
  To compare cellular populations of CD3+ CD56+, CD4+ CD25+, T lymphocytes (CD4+ and CD8+) and NK cells subsets in preeclamptic and pregnant healthy women.
  
  
  METHODS
  Through flow cytometry antibodies, peripheral blood mononuclear cells were obtained from both groups of patients. CD3+ CD56+, CD4+ CD25+, T lymphocytes (CD4+ and CD8+) and NK cells were identified. Mean and standard deviation, Student ttest and Pearson correlation were calculated to analyze differences between groups and correlation between mean blood pressure and different lymphocytes subsets; p < 0.05 was considered significant.
  
  
  RESULTS
  CD3+ CD56+ cells percentage was lower in preeclamptic patients (2.7 vs 6.1%; p < 0.002), CD4+ CD25+ cells percentage tend to be lower too (22.11 vs 33.86; p = NS). Mean blood pressure shown negative correlation with CD3+ CD56+ cells percentage (rp - 0.666; p = 0.001) and with CD25 on CD4+ T lymphocytes surface (rp - 0.526; p < 0.025).
  
  
  CONCLUSIONS
  Based on the association between mean blood pressure and lymphocytes percentage for these two cellular subsets, data obtained suggest that CD3+ CD56+ and CD4+ CD25+ cells play an important role in preeclampsia development.
authors.affiliation: Centro Médico Nacional de Occidente, UMAE, Hospital de Especialidades, Unidad de Investigación Médica en Epidemiología Clínica, Guadalajara, Jalisco, México. luz.balderas@cucs.udg.mx
authors.forename: Luz Ma Adriana
authors.initials: LM
authors.lastname: Balderas Peña
authors.forename: Carlos Vidal
authors.initials: CV
authors.lastname: Vizcaíno Magaña
authors.forename: Salvador
authors.initials: S
authors.lastname: Hernández Higareda
authors.forename: Cecilia
authors.initials: C
authors.lastname: Vargas Guillén
authors.forename: Fernando
authors.initials: F
authors.lastname: Alvarez Romo
authors.forename: Trinidad
authors.initials: T
authors.lastname: García Iglesias
authors.forename: Susana
authors.initials: S
authors.lastname: del Toro Arreola
authors.forename: Adrián
authors.initials: A
authors.lastname: Daneri Navarro
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets; D011225:Pre-Eclampsia / Q000276:immunology*; D011247:Pregnancy