title: [Study on the determination of bovine serum albumin using o-hydroxyphenylfluorone reagent with fluorescence spectrophotometry].
abstract: The reactions were studied for the determination of bovine serum albumin (BSA) using o-hydroxyphenylfluorone (o-HPF) as fluorescence probe reagent with fluorescence spectrophotometry. In the B-R medium, o-HPF reacts with BSA to form the stable complex compound. The reaction system of o-HPF and BSA was binary system, and the method for the determination of BSA was of good selectivity and stability and simplicity. The linear range of o-HPF fluorescence spectrophotometric method was 1.32-18.54 microg x mL(-1) The quenching constant and thermodynamics constant of the fluorescence effects between BSA and o-HPF were calculated. The results showed that the non-covalent binding forces were the binding force between o-HPF and BSA.
authors.affiliation: China Three Gorges University Alan G. MacDiarmid Institute for Renewable Energy, Yichang 443002, China. huangyp@ctgu.edu.cn
authors.forename: Ying-ping
authors.initials: YP
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Gu
authors.forename: Yan-fen
authors.initials: YF
authors.lastname: Fang
authors.forename: Yu-liang
authors.initials: YL
authors.lastname: Liu
authors.forename: Sheng-ji
authors.initials: SJ
authors.lastname: Zhu
authors.forename: Guang-fu
authors.initials: GF
authors.lastname: Lou
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature; D013816:Thermodynamics
chemical_list: D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine