title: Synthesis of oligonucleotides terminally modified by bulky and negatively charged substituents and their hybridization with RNA targets.
abstract: 2'-O-methyl RNAs having phosphorylated cyclohexane groups at the 5' and 3'-terminal bases were synthesized and their hybridization properties toward RNA targets were studied. It was proved that the duplex of the modified 2'-O-methyl RNAs and the target RNA longer than the 2'-O-methyl RNA was less stable than that with the short target. In addition, it was also clarified that the selectivity toward the short target was improved by the introduction of the phosphate group in comparison with that without the phosphate group.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takaku
authors.forename: Kazuya
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Sayako
authors.initials: S
authors.lastname: Kurohagi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D003839:Deoxyadenosines / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D012313:RNA / Q000737:chemistry*
chemical_list: D003839:Deoxyadenosines; D015345:Oligonucleotide Probes; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D012313:RNA