Characterization of gene expression regulated by human OTK18 using Drosophila melanogaster as a model system for innate immunity.
OTK18 is a human transcriptional suppressor implicated in the regulation of human immunodeficiency virus type-one infection of mononuclear phagocytes. It is ubiquitously expressed in all normal tissues, but its normal homeostatic function is yet to be characterized. One hypothesis is that OTK18 aids in the regulation of the innate immune system. To test this hypothesis, cDNA microarray analysis was performed on the total RNA extracted from Drosophila melanogaster embryonic Schneider 2 (S2) cells transfected with either pEGFP-OTK18 (enhanced green fluorescent protein) or empty vector controls (pEGFP-N3) for 6, 12 and 24 h. cDNA microarray analysis revealed differential expression of genes known to be important in regulation of Drosophila innate immunity. The expression levels of two genes, Metchnikowin and CG16708 were verified by quantitative real-time reverse transcription PCR. These results suggest a role for OTK18 in innate immunity.
Spresser
Cole R
CR
Department of Biology, University of Nebraska at Kearney, Kearney, NE 68849, USA.
Marshall
Sarah E
SE
Carlson
Kimberly A
KA
D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C104178:ZNF175 protein, human; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
Fly (Austin). 2007 Jan-Feb;1(1):47-54
18690062
Pediatr Res. 2005 May;57(5 Pt 2):104R-109R
15817497
J Clin Invest. 1998 Sep 1;102(5):874-80
9727055
J Biol Chem. 1996 Feb 23;271(8):4038-45
8626737
Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9764-9
17535917
Neuron. 2002 May 16;34(4):509-19
12062036
J Gen Virol. 2007 Jan;88(Pt 1):236-241
17170456
Genetics. 2002 Nov;162(3):1283-99
12454073
Genetics. 1998 Jul;149(3):1465-75
9649534
Cell. 1997 Feb 21;88(4):553-60
9038346
Cell. 2002 Sep 6;110(5):625-37
12230979
Annu Rev Genet. 2005;39:153-71
16285856
Brain Pathol. 1998 Oct;8(4):695-7
9804378
BMJ. 2001 Jun 23;322(7301):1536-8
11420279
Immunol Rev. 2004 Apr;198:169-84
15199962
Dev Biol. 1999 Nov 15;215(2):358-74
10545243
Clin Exp Metastasis. 1999;17(10):857-63
11089884
J Immunol. 2004 Jan 1;172(1):381-91
14688346
Nat Rev Genet. 2001 Apr;2(4):256-67
11283698
Cell. 1998 Jun 12;93(6):939-49
9635424
Immunity. 2000 Nov;13(5):737-48
11114385
Mech Dev. 1995 Jun;51(2-3):317-27
7547477
J Biol Chem. 1998 Aug 7;273(32):20438-47
9685398
Immunogenetics. 1994;39(4):230-42
8119729
Science. 1999 Apr 30;284(5415):770-6
10221902
J Neuroimmunol. 2004 May;150(1-2):186-98
15081264
Am J Hum Genet. 1998 Jan;62(1):10-4
9443887
Science. 1996 Apr 5;272(5258):50-3
8600536
J Cell Sci. 2001 Aug;114(Pt 15):2787-94
11683412
Nature. 2003 Nov 6;426(6962):33-8
14603309
Genomics. 1996 Feb 1;31(3):376-9
8838321
Nucleic Acids Res. 2007 Jan;35(Database issue):D486-91
17099233
Nat Rev Genet. 2003 Mar;4(3):181-94
12610523
Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9541-6
9689116
Mol Neurobiol. 2001 Feb;23(1):21-51
11642542
Curr Biol. 2003 Feb 18;13(4):358-63
12593804
Annu Rev Neurosci. 2003;26:627-56
12704223
EMBO J. 2005 Oct 5;24(19):3423-34
16163390
Nature. 2000 Mar 23;404(6776):394-8
10746727