title: Methods and applications of laser-enabled analysis and processing (LEAP).
abstract: The LEAP (Laser-Enabled Analysis and Processing) platform combines in situ imaging with laser manipulation to efficiently identify, purify, and monitor expansion of high secreting clones. It also allows for rapid analysis of cell population heterogeneity. This unit describes the LEAP instrumentation as well as basic and alternate protocols of the major applications in recombinant human or humanized IgG expression. The protocols include fluorescent cell counting, secreted recombinant IgG capture and detection, and IgG-secreting clone selection by laser processing.
authors.affiliation: Cell Sciences and Development, SAFC Biosciences, St. Louis, Missouri, USA.
authors.forename: Nan
authors.initials: N
authors.lastname: Lin
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Cresswell
authors.forename: Genova A
authors.initials: GA
authors.lastname: Richardson
authors.forename: Mark A
authors.initials: MA
authors.lastname: Gerber
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Kayser
mesh_terms: D002448:Cell Adhesion; D002452:Cell Count; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002999:Clone Cells; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics / Q000378:metabolism; D047569:Laser Scanning Cytometry / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D011994:Recombinant Proteins