title: Yellow lupine cyclophilin interacts with nucleic acids.
abstract: To investigate properties of yellow lupine cytosolic cyclophilin, an expression vector pET15CYP was constructed. The CyP cDNA (GenBank accession no.Y16088) reveals an open reading frame of 172 amino acids with the conserved tryptophan residue at position 128 and an insertion of seven amino acids spanning positions 48-54. Yellow lupine cyclophilin, purified after expression in E. coli cells, exhibits peptidyl-prolyl cis/trans isomerase activity when assayed with a synthetic oligopeptide. We have demonstrated that the recombinant cyclophilin is able to interact with nucleic acids, both single and double stranded DNA fragments as well as RNA.
authors.affiliation: University of Natural Sciences, Department of Biochemistry and Biotechnology, ul. Wołyńska 35, 60-637 Poznań, Poland. ktnuc@owl.au.poznan.pl
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Nuc
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Leśniewicz
authors.forename: Przemysław
authors.initials: P
authors.lastname: Nuc
authors.forename: Ryszard
authors.initials: R
authors.lastname: Słomski
mesh_terms: D001483:Base Sequence; D021983:Cyclophilins / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D005822:Genetic Vectors; D007700:Kinetics; D031297:Lupinus / Q000201:enzymology* / Q000235:genetics; D009696:Nucleic Acids / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D009696:Nucleic Acids; D011994:Recombinant Proteins; D021983:Cyclophilins