title: Granzyme A causes detachment of alveolar epithelial A549 cells accompanied by promotion of interleukin-8 release.
abstract: Granzyme A (GrA) is a serine protease produced in cytotoxic lymphocytes, lung epithelial cells (alveolar type-II cells), and alveolar macrophages. In the present study, recombinant rat GrA (rGrA) was found to cause rounding and detachment of an alveolar type-II epithelial cell line, A549. Also, rGrA stimulated release of a neutrophil chemoattractant, interleukin-8, from the cells, via a mechanism involving microtubule disruption, probably resulting from reduction of cell adhesion to culture dishes. These findings suggest that GrA might be involved in the pathogenesis of certain lung diseases characterized by loss of alveolar wall structures, neutrophil accumulation, and chronic inflammation.
authors.affiliation: Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirayasu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsuzuki
authors.forename: Tohru
authors.initials: T
authors.lastname: Fushiki
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned / Q000737:chemistry; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D003603:Cytotoxins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism*; D053804:Granzymes / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
chemical_list: D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D003603:Cytotoxins; D016209:Interleukin-8; D011994:Recombinant Proteins; D053804:Granzymes