title: Analysis of modified nucleosides in the urine of patients with malignant cancer by liquid chromatography/electrospray ionization mass spectrometry.
abstract: As modified nucleosides reflect altered tRNA turnover which seems to be impaired in the body of cancer patients, they have been evaluated as potential tumor markers. High-performance liquid chromatography/electrosprary ionization quadrupole time-of-flight mass spectrometry (HPLC/ESI-Q-TOFMS) was used to identify nucleosides purified from urine in positive ionization mode. Potential nucleosides were assessed by their evident UV absorbance in HPLC and then further examined by mass spectrometric techniques. In this manner, 21 nucleosides were detected in the urine of a patient with lymphoid cancer including three modified nucleosides 5'-dehydro-2-deoxyinosine, N1,N2,N7-trimethylguanosine and N1-methyl-N2-ethylguanosine, which had never been reported previously.
authors.affiliation: Department of Chemistry, Zhengzhou University, 100 Science Road, 450001 Zhengzhou, China.
authors.forename: Hua-Yu
authors.initials: HY
authors.lastname: Li
authors.forename: Shao-Min
authors.initials: SM
authors.lastname: Wang
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Han
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Bu
authors.forename: Ming-Zhi
authors.initials: MZ
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D009369:Neoplasms / Q000652:urine*; D009705:Nucleosides / Q000652:urine*; D012334:RNA, Neoplasm / Q000652:urine; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007202:Indicators and Reagents; D009705:Nucleosides; D012334:RNA, Neoplasm