Transmission of Y chromosomes from XY female mice was made possible by the replacement of cytoplasm during oocyte maturation.
The B6.Y(TIR) sex-reversed female mouse is anatomically normal at young ages but fails to produce offspring. We have previously shown that its oocytes go through the meiotic cell cycle up to the second metaphase; however, the meiotic spindle is not properly organized, the second meiotic division goes awry after activation or fertilization, and none of the oocytes initiate embryonic development. In the present study, we transferred the nuclei of GV-stage oocytes from XY females into the enucleated GV-stage oocytes from (B6.DBA)F1.XX females. The resultant reconstructed oocytes properly assembled second meiotic spindles after in vitro maturation and produced healthy offspring after in vitro fertilization. Some male pups inherited maternal Y chromosomes. We conclude that the cytoplasm of the XY oocyte is insufficient to support spindle formation at the second metaphase whereas its replacement with the cytoplasmic material from an XX oocyte allows normal development.
Obata
Yayoi
Y
Department of Bioscience, Tokyo University of Agriculture, 1-1-1, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Villemure
Michele
M
Kono
Tomohiro
T
Taketo
Teruko
T
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D007621:Karyotyping; D008297:Male; D008540:Meiosis; D051379:Mice; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D011110:Polymorphism, Genetic / Q000235:genetics; D008941:Spindle Apparatus / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014960:X Chromosome / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*
D004268:DNA-Binding Proteins; D014157:Transcription Factors; C501342:Zfy1 protein, mouse
Nat Genet. 1996 May;13(1):91-4
8673112
Cell. 2007 Aug 10;130(3):484-98
17693257
J Cell Biol. 2002 May 13;157(4):603-13
12011110
Genome. 1988 Dec;30(6):870-8
3234755
Reprod Biomed Online. 2002 Sep-Oct;5(2):117-24
12419035
Biol Reprod. 1998 Feb;58(2):574-82
9475416
Development. 1998 Apr;125(8):1553-60
9502736
Hum Reprod. 1996 Oct;11(10):2217-22
8943533
Cell Cycle. 2007 Feb 1;6(3):330-8
17297311
Nat Genet. 2005 Dec;37(12):1351-5
16258540
Hum Genet. 1985;70(1):11-7
3997148
Chromosoma. 2000 Nov;109(7):435-8
11151672
Hum Reprod. 1999 May;14(5):1312-7
10325284
Science. 1982 Aug 6;217(4559):535-7
7089579
Dev Biol. 1994 Oct;165(2):442-52
7958412
Development. 2003 Nov;130(21):5169-77
12944431
Mol Reprod Dev. 1998 Jun;50(2):240-9
9590541
Fertil Steril. 1999 Apr;71(4):726-31
10202887
Hum Reprod Update. 2001 Jul-Aug;7(4):428-35
11476356
Dev Cell. 2006 Apr;10(4):521-9
16580996
J Reprod Dev. 2007 Dec;53(6):1183-90
17827875
Mol Endocrinol. 2001 Jun;15(6):854-66
11376106
Mol Cell Biol. 2005 Feb;25(3):1041-53
15657431
Cytogenet Cell Genet. 2000;89(1-2):38-43
10894933
J Cell Sci. 1991 Oct;100 ( Pt 2):289-98
1721916
Dev Biol. 2007 Jan 1;301(1):1-13
17123505
Development. 1989 Sep;107(1):95-105
2534072
J Cell Sci. 1998 Jun;111 ( Pt 11):1477-81
9580556
Lancet. 1997 Jul 19;350(9072):186-7
9250192
Proc Natl Acad Sci U S A. 1990 Aug;87(16):6049-53
2385583
Mol Cell Biol. 2004 Jun;24(12):5485-95
15169909
Nat Genet. 2006 Jan;38(1):47-53
16341221
Chromosoma. 1973;41(2):145-58
4120886
Reproduction. 2008 Feb;135(2):241-52
18239052
Reprod Biomed Online. 2002 Jul-Aug;5(1):26-35
12470542
Development. 1997 Jan;124(2):493-503
9053325
Dev Biol. 1999 Mar 1;207(1):229-38
10049577
Dev Biol. 1996 Sep 15;178(2):263-75
8812128
Lancet. 1991 Jun 8;337(8754):1375-7
1674764
Science. 1989 Jan 6;243(4887):80-3
2563174
Nat Genet. 2005 Jan;37(1):41-7
15580272
Differentiation. 1987;33(3):223-31
3596084
Genetics. 2003 May;164(1):277-88
12750339
Dev Dyn. 2005 Jun;233(2):612-22
15789443
Dev Genet. 1994;15(5):435-42
7955569
Reproduction. 2007 Jun;133(6):1069-72
17636161