title: High-throughput cytotoxicity screening by propidium iodide staining.
abstract: This unit describes a system for the automated high-throughput analysis of cell cytotoxicity in 96-well and 384-well microplates. Discrete cell cultures are analyzed at rates of 40/min (approximately 2.5 min/96 wells, approximately 10 min/384 wells) and cytotoxicity is quantified on the basis of a combination of propidium iodide (PI) fluorescence analysis and cell counting performed by the flow cytometer. Only 2 microl is aspirated from a culture for analysis so that assays can be performed in small volumes to minimize reagent cost and usage.
authors.affiliation: University of New Mexico, Albuquerque, New Mexico, USA.
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Edwards
authors.forename: Irena
authors.initials: I
authors.lastname: Ivnitski-Steele
authors.forename: Susan M
authors.initials: SM
authors.lastname: Young
authors.forename: Virginia M
authors.initials: VM
authors.lastname: Salas
authors.forename: Larry A
authors.initials: LA
authors.lastname: Sklar
mesh_terms: D001331:Automation; D002452:Cell Count; D002460:Cell Line; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival / Q000187:drug effects*; D003470:Culture Media; D004317:Doxorubicin / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D011419:Propidium / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D011419:Propidium; D004317:Doxorubicin