title: Talc pleurodesis: evidence of systemic inflammatory response to small size talc particles.
abstract: The mechanisms of the systemic response associated with talc-induced pleurodesis are poorly understood. The aim of this study was to assess the acute inflammatory response and migration of talc of small size particles injected in the pleural space. Rabbits were injected intrapleurally with talc solution containing small or mixed particles and blood and pleural fluid samples were collected after 6, 24 or 48 h and assayed for leukocytes, neutrophils, lactate dehydrogenase, IL-8, VEGF, and TGF-beta. The lungs, spleen, liver and kidneys were assessed to study deposit of talc particles. Both types of talc produced an acute serum inflammatory response, more pronounced in the small particles group. Pleural fluid IL-8 and VEGF levels were higher in the small particle talc group. Correlation between pleural VEFG and TGF-beta levels was observed for both groups. Although talc particles were demonstrated in the organs of both groups, they were more pronounced in the small talc group. In conclusion, intrapleural injection of talc of small size particles produced a more pronounced acute systemic response and a greater deposition in organs than talc of mixed particles.
authors.affiliation: Laboratory of Pleura, Disciplina de Pneumologia, Heart Institute (InCor), Faculdade de Medicina da Universidade de Sao Paulo (FMUSP), Av. Dr. Eneas de Carvalho Aguiar, Sao Paulo, Brazil. ehgenofre@uol.com.br
authors.forename: Eduardo H
authors.initials: EH
authors.lastname: Genofre
authors.forename: Francisco S
authors.initials: FS
authors.lastname: Vargas
authors.forename: Milena M P
authors.initials: MM
authors.lastname: Acencio
authors.forename: Leila
authors.initials: L
authors.lastname: Antonangelo
authors.forename: Lisete R
authors.initials: LR
authors.lastname: Teixeira
authors.forename: Evaldo
authors.initials: E
authors.lastname: Marchi
mesh_terms: D000210:Acute-Phase Reaction / Q000276:immunology; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D007267:Injections; D016209:Interleukin-8 / Q000032:analysis / Q000097:blood; D007668:Kidney; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000097:blood; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology; D008099:Liver; D008168:Lung; D009504:Neutrophils / Q000276:immunology; D010316:Particle Size; D010996:Pleural Effusion / Q000276:immunology*; D010998:Pleurisy / Q000097:blood / Q000276:immunology; D018700:Pleurodesis / Q000009:adverse effects* / Q000379:methods; D011817:Rabbits; D013154:Spleen; D013627:Talc / Q000032:analysis / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000097:blood
chemical_list: D015415:Biomarkers; D016209:Interleukin-8; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D013627:Talc; D007770:L-Lactate Dehydrogenase