title: New insights into intra- and intermolecular interactions of immunoglobulins: crystal structure of mouse IgG2b-Fc at 2.1-A resolution.
abstract: The structure of the Fc fragment of monoclonal antibody IgG2b from hybridom M75 of Mus musculus has been determined by single crystal X-ray diffraction. This is the first report of the structure of the murine immunoglobulin isotype IgG2b. The structure refined at 2.1 A resolution provides more detailed structural information about native oligosaccharides than was previously available. High-quality Fourier maps provide a clear identification of alpha-l-fucose with partial occupancy in the first branch of the antennary oligosaccharides. A unique Fc:Fc interaction was observed at the C(H)2-C(H)3 interface.
authors.affiliation: Department of Structure Analysis, Institute of Macromolecular Chemistry AS CR, Praha, Czech Republic. hasek@imc.cas.cz
authors.forename: Petr
authors.initials: P
authors.lastname: Kolenko
authors.forename: Jan
authors.initials: J
authors.lastname: Dohnálek
authors.forename: Jarmila
authors.initials: J
authors.lastname: Dusková
authors.forename: Tereza
authors.initials: T
authors.lastname: Skálová
authors.forename: Renata
authors.initials: R
authors.lastname: Collard
authors.forename: Jindrich
authors.initials: J
authors.lastname: Hasek
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000936:Antigen-Antibody Complex / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D006031:Glycosylation; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D051379:Mice; D009844:Oligosaccharides / Q000737:chemistry; D017433:Protein Structure, Secondary
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D009844:Oligosaccharides
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