Acute phase protein response in goats.
Acute phase proteins (APPs) are important diagnostic indicators of inflammatory disturbances in animals. The objectives of the current study were to validate analytical methods for measuring haptoglobin (Hp), serum amyloid A (SAA), acid soluble glycoprotein (ASG), fibrinogen, and albumin concentrations in goats and to evaluate their response to an inflammatory stimulus in this species. Intra- and interassay coefficients of variation (CVs) were in the range 0.07-9.31% and 1.83-12.68%, respectively, for all APPs and showed good precision. All assays determined APPs in a linear manner at different sample dilutions with high correlation coefficients with the exception of fibrinogen, which was measured by the heat precipitation method. Subcutaneous injection of turpentine oil induced an increase in Hp, SAA, ASG, and fibrinogen serum concentrations and a decrease in albumin concentration.
González
Félix H D
FH
Veterinary School, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Tecles
Fernando
F
Martínez-Subiela
Silvia
S
Tvarijonaviciute
Asta
A
Soler
Laura
L
Cerón
José J
JJ
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000818:Animals; D005340:Fibrinogen / Q000378:metabolism; D015511:Goat Diseases / Q000097:blood*; D006041:Goats; D006242:Haptoglobins / Q000378:metabolism; D007249:Inflammation / Q000097:blood / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000378:metabolism; D000685:Serum Amyloid A Protein / Q000378:metabolism
D000209:Acute-Phase Proteins; D006242:Haptoglobins; D012709:Serum Albumin; D000685:Serum Amyloid A Protein; D005340:Fibrinogen