Lipid/polydiacetylene films for colorimetric protein surface-charge analysis.
The distribution and organization of charges on a protein surface are fundamental properties which affect protein functions and interactions. We demonstrate a new approach for protein surface-charge analysis through modulating protein interactions with chromatic lipid/polydiacetylene (PDA) films. We show that visible and easily quantifiable blue-red transitions, induced on the film surface through electrostatic interactions between the negatively charged PDA and positive soluble species, constitute an effective means for characterizing protein surface charge. Specifically, protein-film interactions can be significantly modulated by complexation between the tested macromolecules and lipid-embedded multivalent calixarene ligands displaying charged residues, making possible protein discrimination based upon the abundance and organization of surface charge. The lipid/PDA film system, in conjunction with the calixarene-derived ligands, facilitates characterization of protein surface charges and identification of anomalous protein electrostatic properties.
Friedman
Sarit
S
Department of Chemistry and Ilse Katz Institute for Nanotechnology, Ben Gurion University, Beer Sheva, Israel 84105.
Kolusheva
Sofiya
S
Volinsky
Roman
R
Zeiri
Leila
L
Schrader
Thomas
T
Jelinek
Raz
R
D000818:Animals; D047250:Calixarenes / Q000737:chemistry; D002417:Cattle; D003124:Colorimetry / Q000379:methods*; D008024:Ligands; D010743:Phospholipids / Q000737:chemistry*; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012995:Solubility; D055672:Static Electricity; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D008024:Ligands; D010743:Phospholipids; D000078789:Polyacetylene Polymer; D011108:Polymers; D011506:Proteins; D014867:Water; D047250:Calixarenes; D053279:Polyynes; C082361:polydiacetylene