Genomic expression analysis by single-cell mRNA differential display of quiescent CD8 T cells from tumour-infiltrating lymphocytes obtained from in vivo liver tumours.
We performed a genomic study combining single-cell mRNA differential display and RNA subtractive hybridization to elucidate CD8 T-cell quiescence/ignorance. By comparing actively maintained quiescent CD8 T cells from liver tumour tumour-infiltrating lymphocytes (TILs) with quiescent T cells at the single-cell level, we identified differentially expressed candidate genes by high-throughput screening and comparative analysis of expressed sequence tags (ESTs). While genes for the T-cell receptor, tumour necrosis factor (TNF) receptor, TNF-related apoptosis inducing ligand (TRAIL) and perforin were down-regulated, key genes such as Tob, transforming growth factor (TGF)-beta, lung Krüpple-like factor (LKLF), Sno-A, Ski, Myc, Ets-2 repressor factor (ERF) and RE1-silencing transcription factor (REST/NRSF) complex were highly expressed in the quiescent TIL CD8 cells. Real-time polymerase chain reaction (PCR) further confirmed these results. A regulation model is proposed for actively maintained quiescence in CD8 T cells, including three components: up-regulation of the TGF-beta pathway, a shift in the MYC web and inhibition of the cell cycle.
Zhang
Wei
W
Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106-4935, USA.
Ding
Jianqing
J
Qu
Yan
Y
Hu
Hongliang
H
Lin
Meihua
M
Datta
Amit
A
Larson
Alan
A
Liu
George E
GE
Li
Biaoru
B
D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D015723:Gene Library; D023281:Genomics; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods
D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011948:Receptors, Antigen, T-Cell
Science. 1994 Jul 22;265(5171):528-30
7518614
J Biol Chem. 2001 Apr 6;276(14):11016-20
11116156
Cancer Res. 2006 Sep 1;66(17):8477-83
16951159
Mol Immunol. 2005 Mar;42(5):627-41
15607822
EMBO J. 1995 Oct 2;14(19):4781-93
7588608
Nat Immunol. 2001 Aug;2(8):667-8
11477398
Biotechniques. 1998 May;24(5):720-2, 724
9591114
Gene. 2000 Sep 19;255(2):273-9
11024287
Crit Rev Immunol. 1998;18(3):255-73
9637413
J Exp Med. 1996 May 1;183(5):2403-7
8642353
J Gastroenterol. 2002;37(4):275-82
11993511
Genes Cells. 2005 Feb;10(2):151-63
15676026
Immunol Res. 2005;33(2):135-47
16234580
In Vivo. 1999 Sep-Oct;13(5):433-8
10654199
Immunol Lett. 1996 Oct;53(1):15-8
8946212
Genes Dev. 2005 Mar 1;19(5):530-5
15741317
Nat Immunol. 2001 Dec;2(12):1174-82
11694881
J Clin Lab Immunol. 1987 Apr;22(4):181-4
3497276
Hum Mol Genet. 2003 Jul 1;12(13):1579-89
12812985
Curr Opin Immunol. 2000 Apr;12(2):206-9
10712937
Blood. 2004 Oct 1;104(7):1970-8
15187028
Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5757-9
11983876
Nature. 2006 Jul 20;442(7100):299-302
16855590
J Cell Biochem. 2006 Dec 1;99(5):1326-33
16795054
J Mol Biol. 2006 Jul 7;360(2):310-28
16762365
Int J Oncol. 1998 Dec;13(6):1135-40
9824621
EMBO J. 2000 May 15;19(10):2280-91
10811619
J Immunol. 2005 Feb 15;174(4):1830-40
15699109
An Acad Bras Cienc. 2001 Sep;73(3):365-83
11600898