Propofol and N-Acetylcysteine attenuate oxidative stress induced by intestinal ischemia/reperfusion in rats: protein carbonyl detection by immunoblotting.
OBJECTIVE
To evaluate the antioxidant effect of Propofol and N-Acetylcysteine (NAC) on intestinal ischemia/reperfusion (I/R) in rats by determining carbonyl protein level.


METHODS
Forty Wistar rats were randomly assigned into the following groups: Control; Sham; I/R with Propofol; I/R with Propofol and NAC; I/R with Ketamine and Xylazine. The I/R groups underwent 60 minutes of ischemia and an equal period of reperfusion. Blood samples, collected by cardiac punction, were centrifuged for plasma obtainment. Protein carbonyl level in plasma samples was determined by immunoblotting.


RESULTS
No significant difference in protein carbonyl level was found between Control and Sham groups (P>0.05). The highest reduction in protein carbonyl level (P<0.05) was obtained with the administration of Propofol and NAC (Group 4) in intestinal I/R procedure.


CONCLUSIONS
The administration of Propofol and NAC showed the best antioxidant effect on oxidative stress in rats that underwent intestinal I/R procedure, suggesting a synergistic interaction.
Azeredo
Marcelo Aragão Insuellas de
MA
Faculty of Medicine, UFRJ, Rio de Janeiro, Brazil.
Azeredo
Luciana Aragão Insuellas de
LA
Eleuthério
Elis Cristina Araújo
EC
Schanaider
Alberto
A
D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007422:Intestines / Q000098:blood supply*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D015742:Propofol / Q000494:pharmacology*; D050050:Protein Carbonylation / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology
D015415:Biomarkers; D016166:Free Radical Scavengers; D000111:Acetylcysteine; D015742:Propofol