Features and applications of bacterial glycosyltransferases: current state and prospects.
The bioactivity of many natural products including valuable antibiotics and anticancer therapeutics depends on their sugar moieties. Changes in the structures of these sugars can deeply influence the biological activity, specificity and pharmacological properties of the parent compounds. The chemical synthesis of such sugar ligands is exceedingly difficult to carry out and therefore impractical to establish on a large scale. Therefore, glycosyltransferases are essential tools for chemoenzymatic and in vivo approaches for the development of complex glycosylated natural products. In the last 10 years, several examples of successful alteration and diversification of natural product glycosylation patterns via metabolic pathway engineering and enzymatic glycodiversification have been described. Due to the relaxed substrate specificity of many sugar biosynthetic enzymes and glycosyltransferases involved in natural product biosynthesis, it is possible to obtain novel glycosylated compounds using different methods. In this review, we would like to provide an overview of recent advances in diversification of the glycosylated natural products and glycosyltransferase engineering.
Luzhetskyy
Andriy
A
Albert-Ludwigs-Universität, Institut für Pharmazeutische Wissenschaften, Freiburg, Germany. andriy.luzhetskyy@pharmazie.uni-freiburg.de
Bechthold
Andreas
A
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D016695:Glycosyltransferases