title: Germinative testicular cells and bone marrow mononuclear cells transplanted to a rat model of testicular degeneration.
abstract: The aim of this study was to evaluate the ability of rat mononuclear bone marrow cells to recover testis cell associations and multiplication in busulfan-treated rats, and to compare these data to germinative testicular cell transplant. The germinative testicular cells were obtained by the trypsin digestion method, and bone marrow cells were harvested from femurs and tibias, and purified using by Ficoll gradient. Cell transplantation was performed by the injection of cells through the efferent ducts into the rete testis in busulfan-treated animals. Fifteen days after transplantation, the recipient rats were sacrificed and the testes were collected and analyzed by histology (hematoxilin-eosin and DAPI staining). Results demonstrated that germ cells transplantation promoted cellular reorganization of seminiferous epithelium 15 days later. On the other hand, no improvement in spermatogenesis regeneration was found after heterologous mononuclear bone marrow cell transplantation.
authors.affiliation: Laboratory of Embryology and Cell Differentiation, Research Center, Hospital de Clínicas de Porto Alegre, Brazil.
authors.forename: Marilise M
authors.initials: MM
authors.lastname: Horn
authors.forename: Ana H
authors.initials: AH
authors.lastname: Paz
authors.forename: Marcos E
authors.initials: ME
authors.lastname: Duarte
authors.forename: Guiherme
authors.initials: G
authors.lastname: Baldo
authors.forename: Maria C
authors.initials: MC
authors.lastname: Belardinelli
authors.forename: Ursula
authors.initials: U
authors.lastname: Matte
authors.forename: Elizabeth O Cirne
authors.initials: EO
authors.lastname: Lima
authors.forename: Eduardo P
authors.initials: EP
authors.lastname: Passos
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation; D002066:Busulfan / Q000494:pharmacology; D004195:Disease Models, Animal; D005854:Germ Cells / Q000637:transplantation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects / Q000502:physiology; D012670:Seminiferous Epithelium / Q000166:cytology / Q000502:physiology; D013091:Spermatogenesis / Q000502:physiology*; D033581:Stem Cell Transplantation; D013733:Testicular Diseases / Q000473:pathology / Q000628:therapy*; D013737:Testis / Q000166:cytology* / Q000502:physiology; D014183:Transplantation, Heterologous
chemical_list: D002066:Busulfan