title: Losses of expression of the antigens A, Lea and Lex and over-expression of Ley in carcinomas and HG-SIL of the uterine cervix.
abstract: BACKGROUND
  The glycosylation of a great number of molecules, glyco-protein or glycolipids, has been of interest for decades.
  
  
  OBJECTIVE
  To compare the expressive patterns of the isoantigenic determinants of histo-blood groups ABH and Lewis in squamous and simple epithelium and in precursors and cancers of the cervix.
  
  
  METHODS
  A total of 36 lesions and neoplasms (10 LG-SIL, 16 HG-SIL and 10 invasive carcinomas) have been studied with immunohistochemical techniques, using monoclonal antibodies (MoAb BG1 to BG8) for precursor chains, blood-group ABH and Lewis group Le(a), Le(b), Le(x), and Le(y), and four types of lectins. In addition, we have studied the expression of p53 protein and PCNA, establishing the rate of proliferation of each lesion. Using PCR techniques, we have also detected part of the intron of the E6 gene of HPV-16.
  
  
  RESULTS
  In the invasive cervical carcinomas, we observed a loss of expression of the Le(x) antigen (p < 0.01). With regard to the progression of the different lesions studied, we found alterations in the patterns of expression of the antigens of the ABH and Lewis blood groups. There was a tendency towards a loss of expression and heterogeneous patterns in the more advanced lesions, as well as over-expression of the Le(y) antigens. With PCNA, we established a proliferative rate which tended to be greater in relation to the progression of the cervix neoplasms.
  
  
  CONCLUSIONS
  These results indicate that there is a relation between the losses of histo-blood groups and the progression of the squamous intraepithelial lesions.
authors.affiliation: Area de Patología, Universidad Rey Juan Carlos, Madrid, Spain. jemoro@uninet.edu
authors.forename: Ernesto
authors.initials: E
authors.lastname: Moro-Rodríguez
authors.forename: Emilio
authors.initials: E
authors.lastname: Alvarez-Fernández
references.citation: APMIS Suppl. 1992;27:149-61
references.pmid: 1520524
references.citation: Cancer. 1986 Dec 1;58(11):2435-9
references.pmid: 2429756
references.citation: Am J Clin Pathol. 1983 Feb;79(2):178-81
references.pmid: 6297292
references.citation: J Pathol. 1993 Jan;169(1):21-6
references.pmid: 8433212
references.citation: J Exp Med. 1988 Jan 1;167(1):225-30
references.pmid: 2826637
references.citation: Acta Cytol. 1986 Sep-Oct;30(5):461-9
references.pmid: 2430389
references.citation: APMIS Suppl. 1992;27:119-29
references.pmid: 1520521
references.citation: Semin Cancer Biol. 1994 Jun;5(3):187-202
references.pmid: 7948947
references.citation: Int J Cancer. 1988 Mar 15;41(3):344-9
references.pmid: 2831158
references.citation: Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1958-62
references.pmid: 1705714
references.citation: Am J Clin Pathol. 1976 Nov;66(5):767-74
references.pmid: 983985
references.citation: Arch Pathol Lab Med. 1990 May;114(5):475-81
references.pmid: 2334254
references.citation: N Engl J Med. 1992 Oct 29;327(18):1272-8
references.pmid: 1328880
references.citation: Acta Pathol Microbiol Immunol Scand A. 1985 May;93(3):149-51
references.pmid: 4013740
references.citation: Lab Invest. 1989 Feb;60(2):305-10
references.pmid: 2644485
references.citation: N Engl J Med. 1993 Oct 28;329(18):1318-27
references.pmid: 8413413
references.citation: APMIS Suppl. 1992;27:181-7
references.pmid: 1520527
references.citation: Arch Pathol. 1969 Mar;87(3):306-14
references.pmid: 5766256
references.citation: APMIS Suppl. 1992;27:18-27
references.pmid: 1520526
references.citation: Nature. 1994 Jun 16;369(6481):574-8
references.pmid: 7911228
references.citation: Arch Pathol Lab Med. 1991 Jan;115(1):42-9
references.pmid: 1846285
references.citation: Obstet Gynecol. 1992 Mar;79(3):328-37
references.pmid: 1310805
references.citation: Gynecol Oncol. 1972 Nov;1(1):26-35
references.pmid: 4669441
references.citation: Cell. 1990 Dec 21;63(6):1129-36
references.pmid: 2175676
references.citation: J Natl Cancer Inst. 1989 Aug 2;81(15):1151-7
references.pmid: 2664192
references.citation: Int J Gynecol Pathol. 1994 Oct;13(4):337-41
references.pmid: 7814195
references.citation: Am J Pathol. 1991 Jul;139(1):131-7
references.pmid: 1713019
references.citation: Vox Sang. 1989;56(1):1-20
references.pmid: 2464874
references.citation: APMIS Suppl. 1992;27:162-72
references.pmid: 1520525
references.citation: N Engl J Med. 1991 Apr 18;324(16):1084-90
references.pmid: 1848917
references.citation: Obstet Gynecol. 1990 Jun;75(6):1032-6
references.pmid: 2342728
references.citation: J Surg Oncol. 1981;16(1):71-7
references.pmid: 7007740
references.citation: Arch Pathol Lab Med. 1976 Jun;100(6):307-14
references.pmid: 57765
references.citation: Nature. 1987 Apr 2-8;326(6112):515-7
references.pmid: 2882423
references.citation: Nature. 1987 Apr 2-8;326(6112):517-20
references.pmid: 2882424
references.citation: Gynecol Oncol. 1986 Oct;25(2):195-203
references.pmid: 3758829
references.citation: Science. 1990 Apr 6;248(4951):76-9
references.pmid: 2157286
references.citation: APMIS Suppl. 1992;27:173-80
references.pmid: 1381596
references.citation: Arch Pathol. 1968 Jul;86(1):12-21
references.pmid: 5690174
references.citation: Nature. 1994 Jun 16;369(6481):520-1
references.pmid: 7911227
references.citation: J Pathol. 1990 Sep;162(1):23-8
references.pmid: 2231188