title: [Autophagy in fibrotic and postoperative remnant liver in rat].
abstract: OBJECTIVE
  To evaluate the characteristics of autophagy in fibrotic and postoperative remnant liver.
  
  
  METHODS
  Male Wistar rats were randomly divided into three groups: control group; fibrosis group, which received the solution of CCl4 in oil twice a week for 5 weeks; and hepatectomy group, which underwent 70% hepatectomy. Liver tissues and plasma were harvested 18 hours after the surgery. The rats' general conditions and plasma liver function were observed. Histopathological characteristics and regeneration were observed with microscope and transmission electron microscope. Qualitative analysis of autophagosome was made base on the data from transmission electron microscope.
  
  
  RESULTS
  Compared with the control group, plasma total protein and albumin level significantly decreased in the fibrosis group (P < 0.01). Proliferating cell nuclear antigen (PCNA) index was 85%-95% in the fibrosis group. Plasma alanine aminotransferase and aspartate aminotransferase levels significantly increased in the hepatectomy group compared with the control group (P < 0.01), while the autophagical index significantly decreased in both the fibrosis group and hepatectomy group compared with the control group (-95%, P < 0.01; -19%, P < 0.05, respectively). PCNA index was 20%-30% in the hepatectomy group.
  
  
  CONCLUSIONS
  Autophagy is weakened after fibrosis and hepatectomy, although it differs between these two processes. Proper regulation of autophagy may help facilitate the recovery of the residual liver function after hepatectomy.
authors.affiliation: Department of Liver Surgery, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Yi-lei
authors.initials: YL
authors.lastname: Mao
authors.forename: Rong-rong
authors.initials: RR
authors.lastname: Chen
authors.forename: Hua-yu
authors.initials: HY
authors.lastname: Yang
authors.forename: Jing-chun
authors.initials: JC
authors.lastname: Zhang
authors.forename: Yi-dan
authors.initials: YD
authors.lastname: Zhang
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Ma
authors.forename: Xin-ting
authors.initials: XT
authors.lastname: Sang
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.forename: Shou-xian
authors.initials: SX
authors.lastname: Zhong
authors.forename: Jie-fu
authors.initials: JF
authors.lastname: Huang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001343:Autophagy; D004195:Disease Models, Animal; D006498:Hepatectomy; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase