[Effects of honokiol on proliferation and apoptosis of human cervical carcinoma cell line Hela in vitro].
OBJECTIVE
To assess the effects of honokiol on proliferation and apoptosis of human cervical carcinoma cell line Hela in vitro.


METHODS
Cultured HeLa cells were treated with different concentrations of honokiol for the varieties of period (24, 48, 72, 96 h). Cell proliferation was assessed by MTT colorimetric assay. Cell apoptosis was determined by flow cytometry (FCM), Hoechst 33258 fluorescent staining and DNA ladder respectively.


RESULTS
MTT assay demonstrated that the proliferation of Hela cells were suppressed significantly by honokiol in dose-and time-dependent manner. FCM analysis showed that the apoptosis rates of Hela cells treated with 10 microg/mL and 20 microg/mL honokiol for 24 h were 22.5% and 62.2%, respectively, while that of the control group cells was 8.7%. After treatment with honokiol, typically morphologic changes of apoptosis were observed by Hoechst 33258 fluorescence staining; Genomic DNA from Hela cells treated with honokiol displayed a characteristic ladder pattern on agarose gel electrophoresis.


CONCLUSIONS
honokiol can inhibit the proliferation and induce apoptosis of human cervical carcinoma cell line Hela.
Yang
Guang-li
GL
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Hou
Wen-li
WL
Fu
A-fu
AF
Zhong
Zhen-hua
ZH
Yin
Xiu-hua
XH
Chen
Li-juan
LJ
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D013997:Time Factors; D000498:Allyl Compounds; D010636:Phenols
D000972:Antineoplastic Agents, Phytogenic; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D017705:Lignans; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols