Laboratory maintenance and cultivation of bacteroides species.
Bacteroides species are Gram-negative, obligate anaerobic bacteria. They are the predominant indigenous bacterial species in the human intestinal tract, where they play an important role in the normal physiology of the host, but they can also be significant opportunistic pathogens. The fact that these are obligate anaerobes is the prevailing feature that affects the methodology used for their cultivation. Several techniques are described for anaerobic culturing; the anaerobic glove box and anaerobic jars are the two techniques most adaptable to a range of research needs. Straightforward methods are presented for propagation on solid media and in broth cultures, and for the long-term storage and maintenance of stock cultures. The Bacteroides species are saccharolytic, which is the second feature of their physiology that impacts cultivation methodology. Several flexible media formulations, including a defined minimal media, are provided that allow the researcher to choose the carbon source best suited for his or her work.
Bacic
Melissa K
MK
Dept of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina, USA.
Smith
C Jeffrey
CJ
D000693:Anaerobiosis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods*; D001439:Bacteroides / Q000187:drug effects / Q000254:growth & development*; D002417:Cattle; D003264:Containment of Biohazards; D015925:Cryopreservation; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods*; D006801:Humans
D000900:Anti-Bacterial Agents; D003470:Culture Media
Nature. 2006 Dec 21;444(7122):1027-31
17183312
Appl Microbiol. 1974 Aug;28(2):251-7
4853401
Plasmid. 1998 Jul;40(1):12-29
9657930
Science. 2001 Feb 2;291(5505):881-4
11157169
Am J Clin Nutr. 1972 Dec;25(12):1324-8
4565349
Nature. 2004 Jan 29;427(6973):441-4
14749831
Appl Microbiol. 1969 Apr;17(4):568-76
4890748
Anaerobe. 2006 Jun;12(3):115-21
16765857
Cell. 2005 Jul 15;122(1):107-18
16009137
Appl Microbiol. 1966 Nov;14(6):985-8
16349706