Effective cleavage of structured RNAs by tandems of 10-23 DNAzymes with 3'-modified oligo(2'-O-methylribonucleotide)-effectors.
Catalytic activity of DNAzymes targeted to IGF-I and MDR1 mRNA can be regulated by the combination of 10-23 DNAzyme with 3'-modified oligo(2'-O-methylribonucleotides) that are favorable as effectors due to their high affinity to RNA and nuclease resistance. We have demonstrated that the DNAzyme constructions designed were able to bind and cleave long structured RNA transcripts effectively under simulated physiological conditions.
Fokina
Alesya
A
Institute of Chemical Biology and Fundamental Medicine SB RAS, 8 Lavrent'ev ave., Novosibirsk 630090, Russia. fokina@niboch.nsc.ru
Novopashina
Daria
D
Meschaninova
Mariya
M
Vorobjeva
Maria
M
Zenkova
Marina
M
Francois
Jean-Christophe
JC
Venyaminova
Alya
A
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D001483:Base Sequence; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D021881:DNA, Catalytic; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I