A new method of synthesis of alkyl beta-glycosides using sucrose as sugar donor.
Cellobiose phosphorylase from Clostridium thermocellum catalyzed the beta-anomer-selective synthesis of alkyl glucosides from cellobiose. Synthesis of alkyl beta-glucoside from inexpensive sucrose using cellobiose phosphorylase and sucrose phosphorylase from Pseudomonas saccharophilia was investigated. By combined use of these two phosphorylases, alkyl beta-glucoside was anomer-selectively synthesized from sucrose and alkyl alcohol.
Kino
Kuniki
K
Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, Tokyo, Japan. kkino@waseda.jp
Satake
Ryoko
R
Morimatsu
Takayuki
T
Kuratsu
Shoko
S
Shimizu
Yu
Y
Sato
Masaru
M
Kirimura
Kohtaro
K
D000438:Alcohols / Q000378:metabolism; D005960:Glucosides / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D006027:Glycosides / Q000138:chemical synthesis* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*
D000438:Alcohols; D005960:Glucosides; D006027:Glycosides; D013395:Sucrose; D005964:Glucosyltransferases; C034714:cellobiose phosphorylase; C025901:sucrose phosphorylase