Investigating the effect of patulin, penicillic acid and EDTA on biofilm formation of isolates from dental unit water lines.
This study investigated the effect of patulin and penicillic acid, two known quorum-sensing inhibitors, and the common biocide ethylenediaminetetraacetic acid (EDTA) on the biofilm formation and auto-inducer (AI)-2 production of three isolates from dental unit water lines, Klebsiella sp., Bacillus subtilis and Bacillus cereus. Penicillic acid on its own had no effect on the biofilm formation of all isolates, whereas in combination with EDTA, it enhanced biofilm formation significantly in Klebsiella sp. and B. cereus. EDTA at concentrations greater than 10 microM promoted biofilm formation in B. cereus and B. subtilis. Patulin was found to promote biofilm formation in B. cereus up to 25 microM. A significant increase in biofilm formation was observed in B. cereus and B. subtilis at concentrations greater than 10 microM of patulin when combined with EDTA. The Vibrio harveyi BB170 AI-2 bioassay showed a positive response for Klebsiella sp. AI-2 production with a maximum fold induction at the late exponential growth phase. Addition of glucose prolonged the AI-2 production phase considerably. No significant effect of patulin, penicillic acid alone as well as in combination with EDTA was observed on AI-2 production by Klebsiella sp. The findings have important implications for the design of biofilm prevention and eradication strategies.
Liaqat
Iram
I
Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, 54590, Pakistan. iramliaq@hotmail.com
Bachmann
Robert Th
RT
Sabri
Anjum Nasim
AN
Edyvean
Robert G J
RG
Biggs
Catherine A
CA
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects / Q000302:isolation & purification; D001412:Bacillus subtilis / Q000187:drug effects / Q000302:isolation & purification; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D018441:Biofilms / Q000187:drug effects*; D003770:Dental Offices; D004492:Edetic Acid / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006714:Homoserine / Q000031:analogs & derivatives / Q000378:metabolism; D007709:Klebsiella / Q000187:drug effects / Q000302:isolation & purification; D007783:Lactones / Q000378:metabolism; D010365:Patulin / Q000494:pharmacology*; D010398:Penicillic Acid / Q000494:pharmacology*; D014871:Water Microbiology
D000900:Anti-Bacterial Agents; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D010365:Patulin; D004492:Edetic Acid; D005947:Glucose; D010398:Penicillic Acid