Detection of tick-borne pathogens by MassTag polymerase chain reaction.
MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.
Tokarz
Rafal
R
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York 10032, USA.
Kapoor
Vishal
V
Samuel
James E
JE
Bouyer
Donald H
DH
Briese
Thomas
T
Lipkin
W Ian
WI
D000709:Anaplasma / Q000302:isolation & purification; D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D001898:Borrelia / Q000302:isolation & purification; D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D013987:Ticks / Q000382:microbiology*; D014481:United States
D017931:DNA Primers
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