title: Microbial lipases: at the interface of aqueous and non-aqueous media. A review.
abstract: In recent times, biotechnological applications of microbial lipases in synthesis of many organic molecules have rapidly increased in non-aqueous media. Microbial lipases are the 'working horses' in biocatalysis and have been extensively studied when their exceptionally high stability in non-aqueous media has been discovered. Stability of lipases in organic solvents makes them commercially feasibile in the enzymatic esterification reactions. Their stability is affected by temperature, reaction medium, water concentration and by the biocatalyst's preparation. An optimization process for ester synthesis from pilot scale to industrial scale in the reaction medium is discussed. The water released during the esterification process can be controlled over a wide range and has a profound effect on the activity of the lipases. Approaches to lipase catalysis like protein engineering, directed evolution and metagenome approach were studied. This review reports the recent development in the field ofnon-aqueous microbial lipase catalysis and factors controlling the esterification/transesterification processes in organic media.
authors.affiliation: Department of Biotechnology, Himachal Pradesh University, Summer-Hill, Shimla 171 005, India.
authors.forename: M L
authors.initials: ML
authors.lastname: Verma
authors.forename: W
authors.initials: W
authors.lastname: Azmi
authors.forename: S S
authors.initials: SS
authors.lastname: Kanwar
mesh_terms: D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D004795:Enzyme Stability; D004952:Esters / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004952:Esters; C081378:solysime; D008049:Lipase