Microcrystalline cellulose induces time-dependent lung functional and inflammatory changes.
We determined whether microcrystalline cellulose (MCC), a component of pharmaceutical tablets, induces pulmonary changes. In vivo [resistive and viscoelastic pressures (DeltaP(1) and DeltaP(2)), static elastance (E(L))] and in vitro [tissue resistance (R), elastance (E), and hysteresivity (eta)] lung mechanics, histology, and bronchoalveolar lavage fluid (BALF) were analyzed 3h, 24h, and 3, 15 and 30 days after intratracheal instillation of saline (C) or MCC in BALB/c mice. DeltaP(1) increased at 3h, remaining higher than C until day 3, while E(L) and DeltaP(2) increased only at 24h. At 3 days all mechanical parameters returned to baseline. R and E increased only at 24h. MCC increased alveolar collapse and the number of neutrophils in BALF at 3h, until 3 and 15 days, respectively. At 3 days MCC migrate from the airways into the parenchyma, where they were observed until 30 days. In conclusion, microcrystalline cellulose yielded an acute and self-limited inflammation that impaired lung mechanics.
Nagato
L K S
LK
Laboratory of Respiration Physiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Lourenço
M G F
MG
Cadete
R A
RA
Leite-Júnior
J H P
JH
Koatz
V L G
VL
Rocco
P R M
PR
Faffe
D S
DS
Zin
W A
WA
D000403:Airway Resistance / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D002482:Cellulose / Q000009:adverse effects*; D005079:Excipients / Q000009:adverse effects*; D007249:Inflammation / Q000139:chemically induced* / Q000503:physiopathology; D016014:Linear Models; D008168:Lung / Q000473:pathology* / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011650:Pulmonary Alveoli / Q000473:pathology; D001261:Pulmonary Atelectasis / Q000139:chemically induced; D011897:Random Allocation; D015656:Respiratory Mechanics; D013997:Time Factors
D005079:Excipients; D002482:Cellulose; C109691:microcrystalline cellulose