title: Isolation and characterization of potential probiotic lactobacilli from pig feces.
abstract: This study examined four lactobacilli isolated from pig feces. Two hundred lactic acid bacteria strains were obtained from pig feces using selective culture media (with vancomycin and bromocresol green; termed LAMVAB agar). Microscopy, the catalase test, Gram-staining, and RAPD-PCR analysis were used to group the bacteria into 20 related clusters. Phenotypic analysis using the API 50 CH test and genotypic analysis of 16S rDNA sequences identified these clusters as representing single strains of each of Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus reuteri. Bacterial survival under the conditions of low pH (2.0) and high concentration (5.0%, w/v) of bile salt was much better than that of the reference strain (Lactobacillus acidophilus ATCC 33199). The isolated bacteria were quite capable of inhibiting the growth of two pathogens, Escherichia coli K88 and Salmonella typhimurium. The high acid-resistance, bile resistance and antagonism against pathogens, suggest that the four lactic acid bacteria isolated from pig feces could prove useful as piglet probiotics.
authors.affiliation: School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Yun
authors.forename: Ki Beom
authors.initials: KB
authors.lastname: Lee
authors.forename: Youn Kyoung
authors.initials: YK
authors.lastname: Sung
authors.forename: Eun Bae
authors.initials: EB
authors.lastname: Kim
authors.forename: Hong-Gu
authors.initials: HG
authors.lastname: Lee
authors.forename: Yun Jaie
authors.initials: YJ
authors.lastname: Choi
mesh_terms: D000818:Animals; D000898:Antibiosis; D001647:Bile Acids and Salts; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000254:growth & development; D005243:Feces / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D010802:Phylogeny; D019936:Probiotics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012486:Salmonella typhimurium / Q000254:growth & development; D013552:Swine / Q000382:microbiology*
chemical_list: D001647:Bile Acids and Salts; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S