Effects of dietary vitamin supplementation and semen collection frequency on hormonal profile during ejaculation in the boar.
To evaluate the effect of dietary and management factors on boar hormonal status during ejaculation, 39 boars were canulated to determine the profiles of luteinizing hormone (LH), follicle-stimulating hormone (FSH), 17beta-estradiol (E2), and testosterone (T) in blood plasma and seminal fluid. Prior to canulation, 18 boars were fed a basal diet (control), whereas the remainder (n=21) were fed a basal diet supplemented with extra vitamins (supplemented). Within each dietary treatment, two regimens of semen collection were used over the 3mo preceding the hormonal evaluation: three times per 2wk (3/2) or three times per wk (3/1). Plasma E2 was lower (P<0.01) before ejaculation (232.5+/-22.6pg/mL) than at the onset of ejaculation (255.2+/-27.1ng/mL). Plasma T increased from 5.14+/-0.72, before ejaculation to 5.87+/-0.86ng/mL at the onset of ejaculation in supplemented boars, whereas it decreased from 5.15+/-0.65 to 4.87+/-0.70ng/mL in controls (diet by time, P<0.05). At the onset of ejaculation, plasma FSH was higher in 3/2 boars (0.436+/-0.06ng/mL) than in 3/1 boars (0.266+/-0.04ng/mL; P<0.05). During ejaculation, plasma LH increased linearly (P<0.01) from 0.59+/-0.07 to 0.97+/-0.10ng/mL, and plasma E2 and T concentrations were correlated (r=0.62, P<0.01). Plasma FSH before and during ejaculation was negatively correlated with sperm production (r=-0.60, P<0.01) and testicular weight (r=-0.50, P<0.01). In conclusion, dietary and management factors had few impacts on hormonal profiles during ejaculation, but homeostasis of some hormones was related to some criteria of reproductive performance in boars.
Audet
I
I
Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, 2000 College Street, Lennoxville STN, Sherbrooke, Que., Canada J1M 1Z3.
Bérubé
N
N
Bailey
J L
JL
Laforest
J-P
JP
Quesnel
H
H
Matte
J J
JJ
D000822:Animal Husbandry; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D019587:Dietary Supplements; D004542:Ejaculation / Q000502:physiology*; D006728:Hormones / Q000097:blood*; D007315:Insemination, Artificial; D008297:Male; D012661:Semen; D013552:Swine / Q000502:physiology*; D013997:Time Factors; D014815:Vitamins / Q000008:administration & dosage*
D006728:Hormones; D014815:Vitamins