2,2'-Pyridoin derivatives protect HL-60 cells against oxidative stress.
Focusing on 2,2'-pyridoin (1, 1,2-di(2-pyridyl)-1,2-ethenediol) and its synthetic derivatives as the lead compound of the potent antioxidative enediol, their protective effect against oxidative stress was evaluated on the HL-60 cell system. 2,2'-Pyridoins showed no remarkable cytotoxic effect on HL-60 cells. The derivatives 1, 2, 3, 5, and 6 inhibited H(2)O(2)-induced cell death and intracellular oxidative stress more significantly than ascorbic acid. Since 2,2'-pyridoins are oxidized to the diketones, 2,2'-pyridils, in a protic solvent, the antioxidant activity of 2,2'-pyridils was also investigated. 2,2'-Pyridils showed antioxidant activity in the cell; however, the activity was lower than that of 2,2'-pyridoins. These results suggested that 2,2'-pyrdoin derivatives can be good cytoprotective agents against oxidative stress.
Hatanaka
Masashi
M
Keio University, Faculty of Pharmacy, Department of Pharmaceutical Sciences, Shibakoen 1-5-30, Minato-ku 105-8512, Tokyo, Japan.
Nishizawa
Chiho
C
Kakinoki
Tomohiro
T
Takahashi
Kyoko
K
Nakamura
Shigeo
S
Mashino
Tadahiko
T
D000975:Antioxidants / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D016923:Cell Death; D016166:Free Radical Scavengers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015394:Molecular Structure; D018384:Oxidative Stress; D011725:Pyridines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*
C532526:2,2'-pyridoin; D000975:Antioxidants; D016166:Free Radical Scavengers; D011725:Pyridines; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid