Expression and trafficking of the gamma subunit of Na,K-ATPase in hypertonically challenged IMCD3 cells.
The gamma subunit (FXYD2) of Na,K-ATPase is an important regulator of the sodium pump. In this investigation we have analysed the trafficking of gamma to the plasma membrane in cultures of inner medullary collecting duct cells (IMCD3) following acute hypertonic challenge and brefeldin A (BFA) treatment. Following hypertonic challenging for 24 hr immunofluorescence labeling revealed initial co-localization of the gamma subunit and 58K Golgi protein in the cytoplasm, but no co-localization of alpha1 and Golgi protein. Exposure of the challenged cells to BFA prevented the subsequent incorporation of gamma into the basolateral plasma membrane. The gamma subunit instead remained in cytoplasmic vesicles while cell proliferation and cell viability decreased simultaneously. Following removal of BFA from the hypertonic medium the IMCD3 cells recovered with distinct expression of gamma in the basolateral membrane. The alpha1 subunit was only marginally influenced by BFA. The results demonstrate that the gamma subunit trafficks to the plasma membrane via the Golgi apparatus, despite the absence of a signal sequence. The results also suggest that the gamma and alpha subunits do not traffic together to the plasma membrane, and that the gamma and alpha subunit have different turnover rates during these experimental conditions.
Pihakaski-Maunsbach
Kaarina
K
Water and Salt Research Center, Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus C, Denmark. kpm@ana.au.dk
Nonaka
Shoichi
S
Maunsbach
Arvid B
AB
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