In vitro selection of RNA aptamer to hemin.
A new RNA aptamer-binding hemin was synthesized by the in vitro selection (SELEX) method. A pool of 103 bases single strand DNAs containing a randomized sequence of 59 bases was synthesized. The pool was incubated with hemin on hemin-immobilized affinity column. Bound RNAs were eluted off with hemin solution and amplified by PCR. After 3 rounds of selection process, the selected RNAs were cloned and sequenced. Some RNA aptamers, which have affinity to hemin, was selected.
Liu
Mingzhe
M
Nano Medical Engineering Laboratory, RIKEN-The Institute of Physical and Chemical Research, Wako-shi, Saitama, 351-0198, Japan.
Kagahara
Takuma
T
Abe
Hiroshi
H
Ito
Yoshihiro
Y
D052157:Aptamers, Nucleotide / Q000378:metabolism*; D006427:Hemin / Q000737:chemistry / Q000378:metabolism*; D052156:SELEX Aptamer Technique
D052157:Aptamers, Nucleotide; D006427:Hemin