Approaches for studying angiogenesis-related signal transduction.
Understanding how extracellular growth factors activate intracellular pathways that promote angiogenesis is a broad area of research. In this chapter, we outline the systematic dissection of vascular endothelial growth factor (VEGF)-mediated activation of endothelial nitric oxide synthase and other downstream targets that are relevant to the angiogenic response. These approaches may also be applied to most other angiogenic-factor signaling cascades.
Derakhshan
Behrad
B
Vascular Biology and Therapeutics Program, Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut, USA.
Harrison
Kenneth D
KD
Miao
Qing Robert
QR
Sessa
William C
WC
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D049449:Luminescence; D019331:NG-Nitroarginine Methyl Ester / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000503:physiopathology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D009573:Nitrites; D042461:Vascular Endothelial Growth Factor A; D052250:Nitric Oxide Synthase Type III; D019331:NG-Nitroarginine Methyl Ester