Combined immunofluorescence and FISH: new prospects for tumor cell detection/identification.
Fluorescence-microscope based methods are presented that allow automatic selection and quantification of immunophenotyped cells and sequential FISH analysis to facilitate molecular cytogenetic analysis of single cells and very small cell populations. The protocols in this unit are particularly useful for studying the biological and genetic makeup of disseminated tumor cells or other rare cells.
Ambros
Peter F
PF
Children's Cancer Research Institute (CCRI), St. Anna Children's Hospital, Vienna, Austria.
Méhes
Gábor
G
D000818:Animals; D019411:Clinical Laboratory Techniques; D020732:Cytogenetic Analysis; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology*