Over-expression of OsDREB genes lead to enhanced drought tolerance in rice.
The DREB transcription factors, which specifically interact with C-repeat/DRE (A/GCCGAC), play an important role in plant abiotic stress tolerance by controlling the expression of many cold or/and drought-inducible genes in an ABA-independent pathway. We have isolated three novel rice DREB genes, OsDREB1E, OsDREB1G, and OsDREB2B, which are homologous to Arabidopsis DREB genes. The yeast one-hybrid assay indicated that OsDREB1E, OsDREB1G, and OsDREB2B can specifically bind to the C-repeat/DRE element. To elucidate the function of respective OsDREB genes, we have stably introduced these to rice by Agrobacterium-mediated transformation. Transgenic rice plants analysis revealed that over-expression of OsDREB1G and OsDREB2B in rice significantly improved their tolerance to water deficit stress, while over-expression of OsDREB1E could only slightly improved the tolerance to water deficit stress, suggesting that the OsDREBs might participate in the stress response pathway in different manners.
Chen
Jian-Qiang
JQ
College of Life Sciences, Beijing Normal University, Beijing, 100875, People's Republic of China.
Meng
Xiu-Ping
XP
Zhang
Yun
Y
Xia
Mian
M
Wang
Xi-Ping
XP
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics; D003001:Cloning, Molecular; D003080:Cold Temperature; D018744:DNA, Plant / Q000378:metabolism; D003681:Dehydration; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D016133:Polymerase Chain Reaction; D020218:Response Elements; D014157:Transcription Factors / Q000235:genetics; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques; D014867:Water; D001616:beta-Galactosidase / Q000378:metabolism
D029681:Arabidopsis Proteins; D018744:DNA, Plant; C114578:DREB1A protein, Arabidopsis; D010940:Plant Proteins; D014157:Transcription Factors; D014867:Water; D001616:beta-Galactosidase