title: Purification and culture of human blood vessel-associated progenitor cells.
abstract: Multilineage progenitor cells, diversely designated as MSC, MAPC, or MDSC, have been previously extracted from long-term cultures of fetal and adult organs (e.g., bone marrow, brain, lung, pancreas, muscle, adipose tissue, and several others). The identity and location, within native tissues, of these elusive stem cells are described here. Subsets of endothelial cells and pericytes, which participate in the architecture of human blood vessels, exhibit, following purification to homogeneity, developmental multipotency. The selection from human tissues, by flow cytometry using combinations of positive and negative cell surface markers, of endothelial and perivascular cells is described here. In addition, a rare subset of myoendothelial cells that express markers of both endothelial and myogenic cell lineages and exhibit dramatic myogenic and cardiomyogenic potential has been identified and purified from skeletal muscle. The culture conditions amenable to the long-term proliferation of these blood vessel-associated stem cells in vitro are also described.
authors.affiliation: Children's Hospital of Pittsburgh of UPMC, Pittsburgh, Pennsylvania, USA.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Crisan
authors.forename: Johnny
authors.initials: J
authors.lastname: Huard
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.forename: Solomon
authors.initials: S
authors.lastname: Yap
authors.forename: Alison
authors.initials: A
authors.lastname: Logar
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Giacobino
authors.forename: Louis
authors.initials: L
authors.lastname: Casteilla
authors.forename: Bruno
authors.initials: B
authors.lastname: Péault
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001808:Blood Vessels / Q000166:cytology*; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology; D005333:Fetus / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D020286:Pericytes / Q000166:cytology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors
chemical_list: D015415:Biomarkers