title: MAA3 (MAGATAMA3) helicase gene is required for female gametophyte development and pollen tube guidance in Arabidopsis thaliana.
abstract: The female gametophyte plays a central role in the sexual reproduction of angiosperms. We previously isolated the maa3 (magatama3) mutant of Arabidopsis thaliana, defective in development of the female gametophyte, micropylar pollen tube guidance, and preventing the attraction of multiple pollen tubes. We here observed that the nucleolus of polar nuclei is small, and that the fusion of polar nuclei often did not occur at the time of pollination. The MAA3 gene encodes a homolog of yeast Sen1 helicase, required for RNA metabolism. It is suggested that MAA3 may regulate RNA molecules responsible for nucleolar organization and pollen tube guidance.
authors.affiliation: Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa-oiwake, Sakyo, Kyoto, 606-8502 Japan. shimizu@botinst.uzh.ch
authors.forename: Kentaro K
authors.initials: KK
authors.lastname: Shimizu
authors.forename: Toshiro
authors.initials: T
authors.lastname: Ito
authors.forename: Sumie
authors.initials: S
authors.lastname: Ishiguro
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Okada
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D002466:Cell Nucleolus; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005718:Gametogenesis / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006579:Heterozygote; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D010641:Phenotype; D053205:Pollen Tube / Q000254:growth & development*; D020365:RNA Helicases / Q000235:genetics*; D018749:RNA, Plant / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D018744:DNA, Plant; D018749:RNA, Plant; C036483:T-DNA; D020365:RNA Helicases
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