Strand invasion properties and serum stability of alpha-tricyclo-DNA.
beta-tricyclo DNA (tcDNA) is a third generation antisense oligonucleotide developed and studied extensively in our laboratory. We recently became interested in the pairing properties of its alpha-anomeric form and observed parallel duplex formation with natural DNA and RNA. These duplexes were of the same level of thermal stability as their natural counterparts. In addition alpha-tcDNA exhibited poor thermal stability of antiparallel duplexes within its own backbone series which makes it a candidate for double strand invasion applications. Here we report on the strand invasion properties of alpha-tc-DNA and its stability in human serum.
Scheidegger
Simon P
SP
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland.
Leumann
Christian J
CJ
D020029:Base Pairing; D004247:DNA / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009691:Nucleic Acid Denaturation; D016376:Oligonucleotides, Antisense / Q000097:blood / Q000737:chemistry*
D016376:Oligonucleotides, Antisense; D004247:DNA