Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR.
Long range distance measurement on RNA allow the determination of RNA folds. Here we report the site specific incorporation of nitroxide spin labels at U,C and A by "on column synthesis". PELDOR (Pulsed Electron Double Resonance) measurements of several RNAs in the range of 2-6 nm were successful.
Frolow
O
O
Institut für Organische Chemie und Chemische Biologie (OCCB), Goethe-Universität Frankfurt am Main, Max-von-Laue Str. 7, D-60438 Frankfurt am Main, Germany.
Endeward
B
B
Schiemann
O
O
Prisner
T F
TF
Engels
J W
JW
D003497:Cyclic N-Oxides / Q000737:chemistry*; D004247:DNA / Q000138:chemical synthesis; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D007455:Iodine / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D009841:Oligonucleotides; D013113:Spin Labels; D012313:RNA; D004247:DNA; D007455:Iodine