Analysis of TPI gene promoter variation in three sub-Saharan Africa population samples.
Population samples from Angola, Mozambique, and S. Tomé e Príncipe were screened for the TPI gene promoter variants -5A-->G, -8G-->A and -24T-->G. Three haplotypes were identified in the three populations: the haplotype -5A-8G-24T (average frequency 65.3%) and two less common haplotypes -5G-8G-24T (average frequency 24.7%) and -5G-8A-24T (average frequency 10.0%). A population sample from Central Portugal showed the haplotype -5A-8G-24T in 139 chromosomes and one subject heterozygous for haplotype -5G-8A-24G. The exact test of sample differentiation among three groups of malaria-infected individuals classified according to the severity of the disease showed no significant differences. We confirmed TPI gene diversity in sub-Saharan Africa, but we could not detect any association between TPI promoter variation and a malarial protective effect. Larger scale epidemiological studies are thus required to clarify this putative mechanism of natural host defense against this worldwide public health problem.
Manco
Licínio
L
Departamento de Antropologia, Universidade de Coimbra, Coimbra, Portugal. lmanco@antrop.uc.pt
Machado
Patrícia
P
Lopes
Dinora
D
Nogueira
Fátima
F
Do Rosário
Virgílio E
VE
Alonso
Pedro L
PL
Varandas
Luís
L
Trovoada
Maria De Jesus
Mde J
Amorim
António
A
Arez
Ana Paula
AP
D017954:Africa South of the Sahara; D005260:Female; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D008288:Malaria / Q000235:genetics; D008297:Male; D044382:Population Groups / Q000235:genetics*; D011174:Portugal; D011401:Promoter Regions, Genetic / Q000235:genetics; D014305:Triose-Phosphate Isomerase / Q000235:genetics*
D014305:Triose-Phosphate Isomerase