Sequence-selective fluorophore formation by furan-conjugated oligodeoxyribonucleotides.
We developed a mutation diagnosis system using a 1,N'-ethenoadenosine forming reaction. Furan-derivatized oligodeoxyribonucleotides were synthesized and fluorescence properties were studied in the presence of complimentary strand under oxidative conditions. Strong emission at 430 nm was observed in the presence of A allele ODN.
Kobori
Akio
A
Department of Biomolecular Engineering, Graduate School of Science and Technolog, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan. akobori@kit.ac.jp
Morita
Junpei
J
Ikeda
Masato
M
Yamayoshi
Asako
A
Murakami
Akira
A
D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004252:DNA Mutational Analysis; D005663:Furans / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature
D005663:Furans; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; C007640:1,N(6)-ethenoadenosine; D004247:DNA; D000241:Adenosine