Optimization of culture medium for the production of phenyllactic acid by Lactobacillus sp. SK007.
Optimization of medium components for the production of PLA by the strain Lactobacillus sp. SK007, which was isolated from Chinese traditional pickles, was carried out using response surface methodology (RSM) based on a uniform design and a central composite rotatable design (CCRD). The final concentration of the optimized medium was 30 g L(-1) glucose, 5 g L(-1) phenylpyruvic acid (PPA), 47 g L(-1) corn steep liquor, 3 g L(-1) K(2)HPO(4), 3 g L(-1) CH(3)COONa, 30 g L(-1) yeast powder and 3 mL L(-1) Tween-80. A maximum PLA yield of 2.30 g L(-1) was achieved, which was significantly higher than previously reported.
Mu
Wanmeng
W
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China. wmmu@jiangnan.edu.cn
Chen
Chao
C
Li
Xingfeng
X
Zhang
Tao
T
Jiang
Bo
B
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism*; D011786:Quality Control
D003470:Culture Media; D007773:Lactates; C530081:O-acetyl-3-phenyllactic acid