Chip is required for posteclosion behavior in Drosophila.
Neurons acquire their molecular, neurochemical, and connectional features during development as a result of complex regulatory mechanisms. Here, we show that a ubiquitous, multifunctional protein cofactor, Chip, plays a critical role in a set of neurons in Drosophila that control the well described posteclosion behavior. Newly eclosed flies normally expand their wings and display tanning and hardening of their cuticle. Using multiple approaches to interfere with Chip function, we find that these processes do not occur without normal activity of this protein. Furthermore, we identified the nature of the deficit to be an absence of Bursicon in the hemolymph of newly eclosed flies, whereas the responsivity to Bursicon in these flies remains normal. Chip interacts with transcription factors of the LIM-HD (LIM-homeodomain) family, and we identified one member, dIslet, as a potential partner of Chip in this process. Our findings provide the first evidence of transcriptional mechanisms involved in the development of the neuronal circuit that regulates posteclosion behavior in Drosophila.
Hari
Padmanabhan
P
Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India.
Deshpande
Mugdha
M
Sharma
Neha
N
Rajadhyaksha
Neha
N
Ramkumar
Nitya
N
Kimura
Ken-ichi
K
Rodrigues
Veronica
V
Tole
Shubha
S
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D002116:Calcitonin / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006458:Hemolymph / Q000378:metabolism; D007447:Invertebrate Hormones / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D010446:Peptide Fragments / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D013997:Time Factors; D014921:Wings, Animal / Q000254:growth & development* / Q000378:metabolism*
C108740:Chi protein, Drosophila; D029721:Drosophila Proteins; D007447:Invertebrate Hormones; D009687:Nuclear Proteins; D010446:Peptide Fragments; D034741:RNA, Small Interfering; D015124:8-Bromo Cyclic Adenosine Monophosphate; C037553:katacalcin; D002116:Calcitonin; C002295:bursicon
Nature. 2007 Jul 12;448(7150):151-6
17625558
Science. 1962 Oct 5;138(3536):27-9
13894424
Development. 2004 Nov;131(21):5319-26
15456728
Genes Dev. 1998 Sep 15;12(18):2912-20
9744867
Annu Rev Neurosci. 2002;25:251-81
12052910
Curr Biol. 2007 Feb 6;17(3):278-85
17276922
Genetics. 2008 Feb;178(2):883-901
18245346
J Neurosci. 2007 Feb 28;27(9):2290-7
17329426
FEBS Lett. 2005 Apr 11;579(10):2171-6
15811337
Mol Cell. 1999 Aug;4(2):259-65
10488341
Mol Cell. 2000 Oct;6(4):781-90
11090617
J Exp Biol. 2002 Sep;205(Pt 17):2555-65
12151362
J Neurosci. 1990 Feb;10(2):403-1
2106014
Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8751-5
15173589
Nature. 1999 Jan 7;397(6714):76-80
9892357
Development. 2000 May;127(9):1823-31
10751171
PLoS Biol. 2007 Feb;5(2):e37
17298176
Trans Am Clin Climatol Assoc. 2003;114:179-89
12813919
PLoS Biol. 2004 Dec;2(12):e408
15550987
Neuron. 2004 Feb 5;41(3):337-50
14766174
Development. 2001 Nov;128(22):4615-22
11714686
J Comp Neurol. 2002 Oct 14;452(2):163-77
12271490
Neuron. 1997 Mar;18(3):397-409
9115734
Development. 1995 Jun;121(6):1769-73
7600992
Mol Cell. 1999 Aug;4(2):267-73
10488342
Curr Biol. 2004 Jul 13;14(13):1208-13
15242619
Proc Natl Acad Sci U S A. 2005 Feb 22;102(8):2820-5
15703293
Dev Biol. 2000 Oct 1;226(1):73-87
10993675
Science. 2008 Jan 18;319(5861):304-9
18202285
EMBO Rep. 2003 Dec;4(12):1132-7
14647207
Development. 1994 Jul;120(7):1805-15
7924988
Genes Dev. 1997 Oct 15;11(20):2729-40
9334334
Development. 1998 Dec;125(23):4757-65
9806924
Science. 2003 Dec 5;302(5651):1765-8
14657498
Neuron. 2006 Nov 9;52(3):425-36
17088209
J Neurosci. 2004 Aug 4;24(31):6986-90
15295034
Science. 1963 Sep 13;141(3585):1057-8
14043350
J Neurosci. 2006 Jan 11;26(2):573-84
16407556
J Exp Biol. 1999 Nov;202(Pt 21):3037-48
10518485
Neuron. 2005 Mar 3;45(5):689-700
15748845
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10637-42
9724756