title: A novel rich source of human mesenchymal stem cells from the debris of bone marrow samples.
abstract: The debris from human bone marrow (BM) samples is generally filtered out and discarded prior to isolation of mesenchymal stem cells (MSCs). The purpose of this study is to develop a method to harvest MSCs from the debris and investigate their biological characteristics compared with the marrow counterparts. The BM tissue fragments were digested with collagenase and this treatment yielded mononuclear cells half to those from the corresponding filtered BM. The frequencies of colony-forming unit-fibroblast in these two cell populations were not significantly different. MSCs of two origins exhibited similar morphological and phenotypic features. Fluorescent dye-dilution assay showed that they grew at comparable rates both in the primary and passaging cultures. Further, they could be induced into osteoblasts, chondroblasts and adipocytes, as revealed by histological and molecular examinations. Thus, BM tissue fragments may serve as a new source of MSCs in the settings of bench experiments and clinical trials.
authors.affiliation: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors.forename: Ji-De
authors.initials: JD
authors.lastname: Jin
authors.forename: Heng-Xiang
authors.initials: HX
authors.lastname: Wang
authors.forename: Feng-Jun
authors.initials: FJ
authors.lastname: Xiao
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Wang
authors.forename: Xiao
authors.initials: X
authors.lastname: Lou
authors.forename: Liang-Ding
authors.initials: LD
authors.lastname: Hu
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Wang
authors.forename: Zi-Kuan
authors.initials: ZK
authors.lastname: Guo
mesh_terms: D001853:Bone Marrow / Q000737:chemistry; D002454:Cell Differentiation / Q000235:genetics; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D017364:Collagenases / Q000737:chemistry; D003907:Dexamethasone / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D012313:RNA / Q000032:analysis / Q000096:biosynthesis
chemical_list: D012313:RNA; D003907:Dexamethasone; D017364:Collagenases