Trapping of intact, singly-charged, bovine serum albumin ions injected from the atmosphere with a 10-cm diameter, frequency-adjusted linear quadrupole ion trap.
High-resolution real-time particle mass measurements have not been achievable because the enormous amount of kinetic energy imparted to the particles upon expansion into vacuum competes with and overwhelms the forces applied to the charged particles within the mass spectrometer. It is possible to reduce the kinetic energy of a collimated particulate ion beam through collisions with a buffer gas while radially constraining their motion using a quadrupole guide or trap over a limited mass range. Controlling the pressure drop of the final expansion into a quadrupole trap permits a much broader mass range at the cost of sacrificing collimation. To achieve high-resolution mass analysis of massive particulate ions, an efficient trap with a large tolerance for radial divergence of the injected ions was developed that permits trapping a large range of ions for on-demand injection into an awaiting mass analyzer. The design specifications required that frequency of the trapping potential be adjustable to cover a large mass range and the trap radius be increased to increase the tolerance to divergent ion injection. The large-radius linear quadrupole ion trap was demonstrated by trapping singly-charged bovine serum albumin ions for on-demand injection into a mass analyzer. Additionally, this work demonstrates the ability to measure an electrophoretic mobility cross section (or ion mobility) of singly-charged intact proteins in the low-pressure regime. This work represents a large step toward the goal of high-resolution analysis of intact proteins, RNA, DNA, and viruses.
Koizumi
Hideya
H
Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
Whitten
William B
WB
Reilly
Peter T A
PT
D000818:Animals; D001274:Atmospheric Pressure; D002021:Buffers; D002417:Cattle; D004867:Equipment Design; D005740:Gases; D007477:Ions; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D002021:Buffers; D005740:Gases; D007477:Ions; D012710:Serum Albumin, Bovine
J Mass Spectrom. 2001 Sep;36(9):1038-52
11599082
J Mass Spectrom. 2004 May;39(5):471-84
15170743
Science. 1989 Oct 6;246(4926):64-71
2675315
Anal Chem. 1988 Oct 15;60(20):2299-301
3239801