Synthesis of a perylene diimide derivative having two ferrocene moieties as an electrochemical indicator for human telomeric DNA tetraplex.
A water-soluble perylene diimide derivative carrying two ferrocene moieties, FPD, was synthesized in two steps as an electrochemical indicator for human telomeric DNA tetraplex. Spectrophotometric binding studies of FPD with AG(3)(T(2)AG(3))(3) revealed its specific binding to tetraplex DNA in 10 mM MES and 1 mM EDTA (pH 6.25) containing 0.10 M KCl. Cyclic voltammograms of AAT CCG TCG AGC AGA G(T(2)AG(3))(4) in an electrolyte containing 20 mM FPN showed single oxidation and reduction peaks based on the ferrocene moieties. The peak current for the oxidation process increased linearly with the scan rate, suggesting the effective concentration of FPN on this electrode.
Fujikawa
Ryota
R
Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu 804-8550, Japan.
Watanabe
Sadayoshi
S
Ohtsuka
Keiichi
K
Sato
Shinobu
S
Takenaka
Shigeori
S
D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D054856:G-Quadruplexes; D006801:Humans; D007094:Imides / Q000138:chemical synthesis / Q000737:chemistry*; D013053:Spectrophotometry; D016615:Telomere / Q000737:chemistry*
D015342:DNA Probes; D005296:Ferrous Compounds; D007094:Imides; D004247:DNA