BAC-derived markers converted from RFLP linked to Phytophthora capsici resistance in pepper (Capsicum annuum L.).
Phytophthora capsici Leonian, an oomycete pathogen, is a serious problem in pepper worldwide. Its resistance in pepper is controlled by quantitative trait loci (QTL). To detect QTL associated with P. capsici resistance, a molecular linkage map was constructed using 100 F(2) individuals from a cross between Capsicum annuum 'CM334' and C. annuum 'Chilsungcho'. This linkage map consisted of 202 restriction fragment length polymorphisms (RFLPs), 6 WRKYs and 1 simple sequence repeat (SSR) covering 1482.3 cM, with an average interval marker distance of 7.09 cM. QTL mapping of Phytophthora root rot and damping-off resistance was performed in F(2:3) originated from a cross between resistant Mexican landrace C. annuum 'CM334' and susceptible Korean landrace C. annuum 'Chilsungcho' using composite interval mapping (CIM) analysis. Four QTL explained 66.3% of the total phenotypic variations for root rot resistance and three 44.9% for damping-off resistance. Of these QTL loci, two were located close to RFLP markers CDI25 on chromosome 5 (P5) and CT211A on P9. A bacterial artificial chromosome (BAC) library from C. annuum 'CM334' was screened with these two RFLP probes to obtain sequence information around the RFLP marker loci for development of PCR-based markers. CDI25 and CT211 probes identified seven and eight BAC clones, respectively. Nine positive BAC clones containing probe regions were sequenced and used for cytogenetic analysis. One single-nucleotide amplified polymorphism (SNAP) for the CDI25 locus, and two SSRs and cleaved amplified polymorphic sequence (CAPS) for CT211 were developed using sequences of the positive BAC clones. These markers will be valuable for rapid selection of genotypes and map-based cloning for resistance genes against P. capsici.
Kim
Hyoun-Joung
HJ
Department of Plant Science, Seoul National University, Seoul, 151-921, South Korea.
Nahm
Seok-Hyeon
SH
Lee
Heung-Ryul
HR
Yoon
Gi-Bo
GB
Kim
Ki-Taek
KT
Kang
Byoung-Cheorl
BC
Choi
Doil
D
Kweon
Oh Yeol
OY
Cho
Myeong-Cheoul
MC
Kwon
Jin-Kyung
JK
Han
Jung-Heon
JH
Kim
Jeong-Ho
JH
Park
Minkyu
M
Ahn
Jong Hwa
JH
Choi
Soon Ho
SH
Her
Nam Han
NH
Sung
Joo-Hee
JH
Kim
Byung-Dong
BD
D002212:Capsicum / Q000235:genetics* / Q000382:microbiology; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant; D007113:Immunity, Innate; D010838:Phytophthora / Q000472:pathogenicity; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
D018744:DNA, Plant; D005819:Genetic Markers
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