title: Tyrosine phosphorylation of plant tubulin.
abstract: Phosphorylation of alphabeta-tubulins dimers by protein tyrosine kinases plays an important role in the regulation of cellular growth and differentiation in animal cells. In plants, however, the role of tubulin tyrosine phosphorylation is unknown and data on this tubulin modification are limited. In this study, we used an immunochemical approach to demonstrate that tubulin isolated by both immunoprecipitation and DEAE-chromatography is phosphorylated on tyrosine residues in cultured cells of Nicotiana tabacum. This opens up the possibility that tyrosine phosphorylation of tubulin could be involved in modulating the properties of plant microtubules.
authors.affiliation: Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Str., 148, 03680 Kiev, Ukraine. cellbio@cellbio.freenet.viaduk.net
authors.forename: Yaroslav
authors.initials: Y
authors.lastname: Blume
authors.forename: Alla
authors.initials: A
authors.lastname: Yemets
authors.forename: Vadym
authors.initials: V
authors.lastname: Sulimenko
authors.forename: Tetyana
authors.initials: T
authors.lastname: Sulimenko
authors.forename: Jordi
authors.initials: J
authors.lastname: Chan
authors.forename: Clive
authors.initials: C
authors.lastname: Lloyd
authors.forename: Pavel
authors.initials: P
authors.lastname: Dráber
mesh_terms: D002457:Cell Extracts; D015151:Immunoblotting; D047468:Immunoprecipitation; D010766:Phosphorylation; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D002457:Cell Extracts; D014404:Tubulin; D014443:Tyrosine
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