title: Studies on inhibition mechanism of transcription factor NF-kappaB and DNA binding by chelator pyrogallol red on the basis of its interaction with metal ions.
abstract: Recent studies have shown that the zinc influences the DNA binding of transcription factor NF-kappaB. So the zinc-interaction of pyrogallol red (PR) an inhibitor of NF-kappaB-DNA binding was studied by isolating the zinc-PR complex. The complex was characterized using IR, UV-visible, 1H NMR, and thermal studies. Binding sites of PR were investigated by molecular modeling using MM+ and PM3 methods and by generating molecular electrostatic map. These studies have confirmed the role of metal ion chelation in the inhibition of NF-kappaB-DNA binding by PR.
authors.affiliation: Green Chemistry Network Centre, Department of Chemistry, University of Delhi, Delhi 7, India. rksharmagreenchem@hotmail.com
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
authors.forename: C
authors.initials: C
authors.lastname: Chelladurai
authors.forename: A D
authors.initials: AD
authors.lastname: Tiwari
authors.forename: H K
authors.initials: HK
authors.lastname: Rajor
authors.forename: S
authors.initials: S
authors.lastname: Mehta
authors.forename: M
authors.initials: M
authors.lastname: Otsuka
mesh_terms: D001665:Binding Sites; D002614:Chelating Agents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D007477:Ions / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit; D011748:Pyrogallol / Q000031:analogs & derivatives* / Q000737:chemistry; D013057:Spectrum Analysis; D055672:Static Electricity; D013329:Structure-Activity Relationship; D015032:Zinc / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D007477:Ions; D008670:Metals; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D011748:Pyrogallol; C058731:pyrogallol sulfonphthalein; D004247:DNA; D015032:Zinc