title: A rapid and hazardous reagent free protocol for genomic DNA extraction suitable for genetic studies in plants.
abstract: Protocols for genomic DNA extraction from plants are generally lengthy, since they require that tissues be ground in liquid nitrogen, followed by a precipitation step, washing and drying of the DNA pellet, etc. This represents a major challenge especially when several hundred samples must be screened/analyzed within a working day. There is therefore a need for a rapid and simple procedure, which will produce DNA quality suitable for various analyses. Here, we describe a time and cost efficient protocol for genomic DNA isolation from plants suitable for all routine genetic screening/analyses. The protocol is free from hazardous reagents and therefore safe to be executed by non-specialists. With this protocol more than 100 genomic DNA samples could manually be extracted within a working day, making it a promising alternative in genetic studies of large-scale genomic screening projects.
authors.affiliation: Agronomy Department, Purdue University, Lilly Hall, 915 West State Street, West Lafayette, IN 47907, USA. skotchon@purdue.edu
authors.forename: Simeon O
authors.initials: SO
authors.lastname: Kotchoni
authors.forename: Emma W
authors.initials: EW
authors.lastname: Gachomo
mesh_terms: D018744:DNA, Plant / Q000302:isolation & purification*; D005821:Genetic Techniques; D018745:Genome, Plant; D010944:Plants / Q000235:genetics*; D012107:Research Design
chemical_list: D018744:DNA, Plant
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