title: Impaired growth of small intestinal epithelium by adrenalectomy in weaning rats.
abstract: Functional maturation of the small intestine occurs during the weaning period in rats. It is known that this development is facilitated by glucocorticoid. However, the effect of glucocorticoid on morphological development of small intestine has yet to be clarified. The present study evaluated the morphological development and cell proliferation of the small intestine in adrenalectomized (ADX) rat pups. To further understand the mechanism of glucocorticoid effects on intestinal development, we examined the localization of the glucocorticoid receptor in the small intestine. Microscopic analysis showed that growth of villi and crypts is age-dependent, and is significantly attenuated in ADX rats compared with sham-operated rats. BrdU-positive cells, i.e. proliferating cells, were primarily observed in crypt compartments and rapidly increased in number during the early weaning period. The increase in BrdU-positive cells could be attenuated by adrenalectomy. The morphological development of small intestine may be associated with increased proliferation of epithelial cells. On the other hand, glucocorticoid receptors were found in epithelial cells of the mid- and lower villi and not in crypts where BrdU-positive cells were localized. These results indicate that the growth of small intestine is attenuated by adrenalectomy, and that glucocorticoid indirectly acts on proliferation of epithelial cells during the weaning period.
authors.affiliation: Department of Bioresource Science, College of Agriculture, Tamagawa University, 6-1-1 Tamagawa-gakuen, Machida, Tokyo 194-8610, Japan. mytto4ac@agr.tamagawa.ac.jp
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyata
authors.forename: Yuji
authors.initials: Y
authors.lastname: Minai
authors.forename: Minoru
authors.initials: M
authors.lastname: Haga
references.citation: J Clin Pathol. 1981 Oct;34(10):1181-6
references.pmid: 7309898
references.citation: Reprod Fertil Dev. 1996;8(1):35-48
references.pmid: 8713721
references.citation: Am J Physiol. 1984 Jun;246(6 Pt 1):G695-9
references.pmid: 6742120
references.citation: Cell Tissue Kinet. 1985 May;18(3):255-62
references.pmid: 3986870
references.citation: Pediatr Res. 1992 Sep;32(3):306-11
references.pmid: 1408467
references.citation: Anat Rec. 1981 Apr;199(4):565-74
references.pmid: 7270915
references.citation: Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):266-71
references.pmid: 14695885
references.citation: Mol Cell Biol. 1997 Jun;17(6):3181-93
references.pmid: 9154817
references.citation: Am J Physiol Gastrointest Liver Physiol. 2005 May;288(5):G897-906
references.pmid: 15826934
references.citation: Pediatr Res. 2001 Jun;49(6):782-8
references.pmid: 11385138
references.citation: J Anal Toxicol. 1985 Jan-Feb;9(1):20-3
references.pmid: 3920444
references.citation: Am J Physiol Gastrointest Liver Physiol. 2004 Jun;286(6):G947-53
references.pmid: 14739144
references.citation: Biochem Biophys Res Commun. 1998 May 29;246(3):765-70
references.pmid: 9618286
references.citation: J Anat. 1972 May;112(Pt 1):27-33
references.pmid: 5086211
references.citation: Mol Cell Biol. 1988 Apr;8(4):1449-59
references.pmid: 3380086
references.citation: Am J Physiol Gastrointest Liver Physiol. 2005 Sep;289(3):G381-7
references.pmid: 16093419
references.citation: Gut. 1996 Oct;39(4):532-8
references.pmid: 8944561
references.citation: Dig Dis Sci. 2006 Apr;51(4):718-23
references.pmid: 16614994
references.citation: Genes Dev. 2003 Jul 15;17(14):1709-13
references.pmid: 12865297
references.citation: Biochim Biophys Acta. 1964 Nov 22;92:305-11
references.pmid: 14249119
references.citation: Gut. 1995 Jun;36(6):864-73
references.pmid: 7615275
references.citation: Am J Physiol. 1993 Feb;264(2 Pt 1):G306-11
references.pmid: 8447413
references.citation: Histochemistry. 1988;89(4):351-6
references.pmid: 3261722
references.citation: J Histochem Cytochem. 1999 Jul;47(7):949-58
references.pmid: 10375383
references.citation: Ann N Y Acad Sci. 1995 Jun 12;761:261-75
references.pmid: 7625725
references.citation: Gut. 2002 Nov;51(5):748-54
references.pmid: 12377819