Application of acetate derivatives for gas chromatography-mass spectrometry: novel approaches on carbohydrates, lipids and amino acids analysis.
The structure of glycoconjugates has been determined by several chromatographic methods, however gas chromatography-mass spectrometry (GC-MS) has been widely used to identify and quantify the volatile trimethylsilyl and fluoroacyl derivatives. Adapting the reduction/acetylation strategies, we had performed the derivatization of all monosaccharide class, as well as amino acids and OH-fatty acids as from different glycoconjugates. Uronic acids gave characteristic ions at m/z 143, 156 and 173, and 19 amino acids derivatives, gave molecular ions [M]+ and daughter ions of [M-59]+ and [M-43]+ on electron impact (EI)-MS, which provide their rapid identification.
Sassaki
Guilherme L
GL
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, Curitiba, Paraná, CEP 81531-980, Brazil. sassaki@ufpr.br
Souza
Lauro M
LM
Serrato
Rodrigo V
RV
Cipriani
Thales R
TR
Gorin
Philip A J
PA
Iacomini
Marcello
M
D000085:Acetates / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008055:Lipids / Q000032:analysis*
D000085:Acetates; D000596:Amino Acids; D002241:Carbohydrates; D008055:Lipids