In vivo translation studies of plant viral RNAs using reporter genes.
This unit describes the analysis of the translation-regulating properties of viral RNAs in cell-based assays using a luciferase reporter system. Electroporation and polyethylene glycol-mediated introduction of luciferase reporter RNA into cowpea protoplasts are also presented. The Commentary section discusses employing the luciferase reporter system to study translational control by the 5'- and 3'-untranslated regions of a viral mRNA. Inoculum RNA quality and half-life after delivery to the protoplasts should be monitored closely for careful investigation of translational regulation elements.
Matsuda
Daiki
D
Oregon State University, Corvallis, Oregon, USA.
Dreher
Theo W
TW
D018274:Electroporation; D007887:Fabaceae / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D049453:Luciferases, Bacterial / Q000235:genetics / Q000378:metabolism; D011092:Polyethylene Glycols; D014176:Protein Biosynthesis; D011523:Protoplasts / Q000378:metabolism / Q000821:virology; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism
D012367:RNA, Viral; D011092:Polyethylene Glycols; D049453:Luciferases, Bacterial