Preneoplastic and neoplastic lesions in the lung, liver and urinary tract of mice exposed to environmental cigarette smoke and UV light since birth.
It is difficult to reproduce the carcinogenicity of cigarette smoke (CS) in animal models. Recently, we showed that exposure of mice to mainstream CS (MCS) for 120 days, starting immediately after birth, resulted in an early and potent carcinogenic response. In parallel, we implemented studies evaluating intermediate biomarkers and tumors in mice exposed to environmental CS (ECS). To this purpose, we used 263 newborn CD-1 mice born from 27 dams. The whole-body exposure to ECS for 120 days, starting within 12 hr after birth, resulted in an early appearance of preneoplastic lesions in lung, which however tended to attenuate after discontinuing exposure. When the experiment was stopped, after 330 days, the number of lung adenomas was higher in ECS-exposed mice as compared to sham-exposed mice, but such increase was statistically significant only in mice co-exposed to smoke and halogen light mimicking solar irradiation. Moreover, exposure to ECS produced extensive histopathological changes, mainly parenchymatous degeneration, in liver. The alterations produced in both lung and liver require that exposure to ECS starts immediately after birth, no effect being observed when exposure started 8 days later. In contrast, induction by ECS of alterations in the urinary tract, such as microadenomas and adenomas in renal pelvis and kidney, papillary hyperplasia of urothelium, and urinary bladder papillomas, were unrelated to the exposure time after birth. The results obtained with ECS cannot be directly compared to those previously obtained with MCS, since the latter involved shorter daily exposures to more massive CS doses.
D'Agostini
Francesco
F
Department of Health Sciences, University of Genoa, Genoa, Italy.
Balansky
Roumen
R
Steele
Vernon E
VE
Ganchev
Gancho
G
Pesce
Carlo
C
De Flora
Silvio
S
D000375:Aging / Q000502:physiology; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D008113:Liver Neoplasms / Q000209:etiology / Q000473:pathology*; D008175:Lung Neoplasms / Q000209:etiology / Q000473:pathology*; D008297:Male; D051379:Mice; D011230:Precancerous Conditions / Q000209:etiology / Q000473:pathology*; D011247:Pregnancy; D015996:Survival Rate; D014026:Nicotiana / Q000737:chemistry*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014571:Urologic Neoplasms / Q000209:etiology / Q000473:pathology*
D014028:Tobacco Smoke Pollution
J Cancer Res Clin Oncol. 1990;116(6):604-8
2254379
Carcinogenesis. 2003 Sep;24(9):1525-32
12844483
Oncogene. 2004 Feb 5;23(5):1166-76
14647414
Nature. 1959 Jun 13;183(4676):1689
13666865
Carcinogenesis. 2007 Oct;28(10):2236-43
17522065
IARC Monogr Eval Carcinog Risks Hum. 1999;71 Pt 1:1-315
10507919
Anticancer Res. 1995 May-Jun;15(3):839-45
7645968
Toxicol Lett. 2004 Mar 14;148(1-2):1-9
15019083
Carcinogenesis. 1989 Jun;10(6):1089-97
2655963
Toxicol Sci. 2004 Oct;81(2):280-92
15213336
Carcinogenesis. 1993 Aug;14(8):1545-8
8353839
Mutagenesis. 2006 Jan;21(1):61-6
16399846
Mutat Res. 2003 Feb-Mar;523-524:237-52
12628522
FASEB J. 2004 Oct;18(13):1559-61
15289447
Carcinogenesis. 1997 Nov;18(11):2035-42
9395199
Exp Toxicol Pathol. 2005 Jul;57 Suppl 1:171-81
16092725
Int J Oncol. 2001 Mar;18(3):607-15
11179494
J Toxicol Sci. 1991 Feb;16 Suppl 1:53-62
1920544
Cancer Lett. 1988 Dec 15;43(3):173-7
3203336
Mutat Res. 2008 Sep 26;644(1-2):38-42
18640134
IARC Monogr Eval Carcinog Risks Hum. 2004;83:1-1438
15285078
Cancer Res. 2003 Feb 15;63(4):793-800
12591728
Toxicol Sci. 2004 Sep;81(1):26-34
15159525
Carcinogenesis. 2005 Nov;26(11):1999-2009
15944214
Cancer Res. 2007 Feb 1;67(3):1001-6
17283132
Carcinogenesis. 1991 Aug;12(8):1409-15
1860162
Mutat Res. 2008 Jul-Aug;659(1-2):137-46
18155953
J Natl Cancer Inst. 1999 Jul 21;91(14):1194-210
10413421
Mutat Res. 2003 Nov;544(2-3):441-9
14644347
Carcinogenesis. 1997 Mar;18(3):575-86
9067559
Exp Lung Res. 2005 Jan-Feb;31(1):3-18
15765916
Inhal Toxicol. 2005 May;17(6):263-76
15814487