Identification of ultraviolet B-sensitive genes in human peripheral blood cells.
Ultraviolet B (UVB) is a serious irritant for the skin and increases a risk for skin cancer. To identify UVB-sensitive genes in peripheral blood, 11 healthy male volunteers were exposed to 0.3 J/cm(2) of narrow-band (NB)-UVB, about half of minimal erythema dose (MED) in Japanese, and gene expression in blood was analyzed at 4 h, 24 h, 4 d and 7 d after the irradiation using microarray carrying oligonucleotide probes for 2,000 stress-responsive genes. RNA prepared before the irradiation was used as a reference control. Microarray analysis identified 21 genes as UVB-responsive genes with a peak at 24 h in 6 subjects, and real-time PCR validated the significant down-regulation of 9 (ABCB10, ATF1, ABCD3, TANK, FAS, SLC30A9, CHUK, CASP1, and ABCE1) out of the 21 genes in 11 subjects. Considering sensitive and characteristic features of 9 marker genes, they may be useful indicators for monitoring systemic response to UVB irradiation.
Jung
Eun Ju
EJ
Departments of Dermatology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.
Kawai
Tomoko
T
Park
Hwan Ki
HK
Kubo
Yoshiaki
Y
Rokutan
Kazuhito
K
Arase
Seiji
S
D000328:Adult; D001773:Blood Cells / Q000378:metabolism* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D015536:Down-Regulation / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects*; D020869:Gene Expression Profiling; D005819:Genetic Markers / Q000528:radiation effects; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D013312:Stress, Physiological / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*; D055815:Young Adult
D005819:Genetic Markers