Immunophenotypic analysis of human mast cells by flow cytometry.
The immunophenotypic identification and enumeration of human bone marrow mast cells represents a clear demonstration of the utility of flow cytometry for rare-event analysis. The basic approach can be applied to a variety of specimens, including bone marrow, peripheral blood, ascitic fluid, and lymphoid tissue such as adenoids. Special emphasis is placed on markers with potential utility for distinguishing between normal, reactive, and pathological mast cells. From the clinical aspect, immunophenotypic analysis of mast cells may have great utility in supporting the diagnosis of tissue involvement in mastocytosis.
Escribano
L
L
Hospital Ramón y Cajal, Mast Cell Unit, Madrid, Spain.
Navalón
R
R
Núñez
R
R
Díaz Agustín
B
B
Bravo
P
P
D000234:Adenoids; D001201:Ascites / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D008407:Mast Cells / Q000166:cytology*; D008415:Mastocytosis / Q000175:diagnosis / Q000276:immunology; D010641:Phenotype