title: Generation of monoclonal antibody against canine neural-cell adhesion molecule.
abstract: A monoclonal antibody, K9BYU, was generated using Escherichia coli recombinant extracellular domain of canine neural-cell adhesion molecule (N-CAM) as an antigen. Immunoreactivity of K9BYU to insect cell recombinant canine N-CAM was demonstrated by Western blotting using Sf9 insect cells transfected with the canine N-CAM gene. In Western blotting against canine brain tissue, K9BYU detected three isoforms of N-CAM that correspond to three major isoforms of human and mouse N-CAM (N-CAM-120, -140, and -180). From these results, K9BYU was considered to be a useful tool for research of canine N-CAM.
authors.affiliation: Canine-Lab. Inc, Kanda-Ogawa-machi, Chiyoda-ku, Tokyo, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Uematsu
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Ayumi
authors.initials: A
authors.lastname: Koike
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yagihara
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hasegawa
authors.forename: Naoaki
authors.initials: N
authors.lastname: Matsuki
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ono
authors.forename: Tsukimi
authors.initials: T
authors.lastname: Washizu
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000276:immunology*; D017931:DNA Primers; D004285:Dogs; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D017931:DNA Primers; D011994:Recombinant Proteins