Translational bypassing without peptidyl-tRNA anticodon scanning of coding gap mRNA.
Half the ribosomes translating the mRNA for phage T4 gene 60 topoisomerase subunit bypass a 50 nucleotide coding gap between codons 46 and 47. The pairing of codon 46 with its cognate peptidyl-tRNA anticodon dissociates, and following mRNA slippage, peptidyl-tRNA re-pairs to mRNA at a matched triplet 5' adjacent to codon 47, where translation resumes. Here, in studies with gene 60 cassettes, it is shown that the peptidyl-tRNA anticodon does not scan the intervening sequence for potential complementarity. However, certain coding gap mutants allow peptidyl-tRNA to scan sequences in the bypassed segment. A model is proposed in which the coding gap mRNA enters the ribosomal A-site and forms a structure that precludes peptidyl-tRNA scanning of its sequence. Dissipation of this RNA structure, together with the contribution of 16S rRNA anti-Shine-Dalgarno sequence pairing with GAG, facilitates peptidyl-tRNA re-pairing to mRNA.
Wills
Norma M
NM
Department of Human Genetics, University of Utah, Salt Lake City, UT, USA.
O'Connor
Michelle
M
Nelson
Chad C
CC
Rettberg
Charles C
CC
Huang
Wai Mun
WM
Gesteland
Raymond F
RF
Atkins
John F
JF
D000595:Amino Acid Sequence; D000818:Animals; D000926:Anticodon / Q000235:genetics*; D017122:Bacteriophage T4 / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D026942:DNA Topoisomerases / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
D000926:Anticodon; D003062:Codon; D012333:RNA, Messenger; D012346:RNA, Transfer, Amino Acyl; C019037:tRNA, peptidyl-; D026942:DNA Topoisomerases
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