title: Transfection activity of layer-by-layer plasmid DNA/poly(ethylenimine) films deposited on PLGA microparticles.
abstract: Layer-by-layer (LbL) assemblies of DNA and polycations on the surface of colloidal templates can be used for gene delivery. Plasmid DNA encoding for secreted alkaline phosphatase (SEAP) was used to deposit LbL films with poly(ethylenimine) (PEI) on the surface of polystyrene and poly(lactide-co-glycolide) microparticles. The formation of LBL films was confirmed by zeta potential analysis and fluorescence and atomic force microscopy techniques. The LbL particles were rapidly internalized in a dose-dependent manner by J774.1 murine macrophages. Transfection activity of the LbL particles was evaluated in J774.1 cells using three different doses (5, 10, 25 particle per cell). The levels of SEAP expression increased with increasing dose but were lower than transfection levels mediated by control PEI/DNA polyplexes at corresponding DNA doses. The LbL particles reported here present a promising platform for delivery of DNA to phagocytic cells.
authors.affiliation: Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI 48202, United States.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kakade
authors.forename: Devika Soundara
authors.initials: DS
authors.lastname: Manickam
authors.forename: Hitesh
authors.initials: H
authors.lastname: Handa
authors.forename: Guangzhao
authors.initials: G
authors.lastname: Mao
authors.forename: David
authors.initials: D
authors.lastname: Oupický
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage*; D018628:Gene Dosage; D015971:Gene Expression Regulation, Enzymologic; D018014:Gene Transfer Techniques; D019344:Lactic Acid / Q000737:chemistry*; D008264:Macrophages; D051379:Mice; D008863:Microspheres; D010957:Plasmids / Q000008:administration & dosage; D011094:Polyethyleneimine / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011137:Polystyrenes / Q000737:chemistry; D014162:Transfection
chemical_list: D011137:Polystyrenes; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011094:Polyethyleneimine; D004247:DNA; D000469:Alkaline Phosphatase
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