title: Synthesis and properties of oligonucleotides containing silylated pyrene derivatives.
abstract: Preparation and properties of the novel fluorescently labelled oligonucleotides, containing the silylated pyrene derivatives are reported. The silylated pyrene derivatives were introduced into both 5'-terminus and C-5 position of the deoxyuridine derivatives. The fluorescent spectra indicated the possibility of the discrimination of the duplex formation by the simple denaturation.
authors.affiliation: Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Tenjincho 1-5-1, Kiryu, Gunma, 376-8515, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Moriguchi
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hattori
authors.forename: Tohru
authors.initials: T
authors.lastname: Sekiguchi
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ichimura
authors.forename: Takahiko
authors.initials: T
authors.lastname: Kato
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozuka
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*; D013936:Thymidine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009943:Organophosphorus Compounds; D011721:Pyrenes; D013936:Thymidine