Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372.
A purified alkaline thermo-tolerant bacterial lipase from Bacillus cereus MTCC 8372 was immobilized on a Poly (MAc-co-DMA-cl-MBAm) hydrogel. The hydrogel showed approximately 94% binding capacity for lipase. The immobilized lipase (2.36 IU) was used to achieve esterification ofmyristic acid and isopropanol in n-heptane at 65 degrees C under continuous shaking. The myristic acid and isopropanol when used at a concentration of 100 mM each in n-heptane resulted in formation of isopropyl myristate (66.0 +/- 0.3 mM) in 15 h. The reaction temperature below or higher than 65 degrees C markedly reduced the formation of isopropyl myristate. Addition of a molecular sieve (3 A x 1.5 mm) to the reaction mixture drastically reduced the ester formation. The hydrogel bound lipase when repetitively used to perform esterification under optimized conditions resulted in 38.0 +/- 0.2 mM isopropyl myristate after the 3rd cycle of esterification.
Verma
M L
ML
Department of Biotechnology, Himachal Pradesh University, Summer-Hill, Shimla 171 005, India.
Chauhan
G S
GS
Kanwar
S S
SS
D019840:2-Propanol / Q000378:metabolism; D001409:Bacillus cereus / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006536:Heptanes / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D015394:Molecular Structure; D009226:Myristates / Q000378:metabolism*; D019814:Myristic Acid / Q000378:metabolism; D013696:Temperature
D004800:Enzymes, Immobilized; D006536:Heptanes; D009226:Myristates; D019814:Myristic Acid; C008205:isopropyl myristate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C028618:n-heptane; D008049:Lipase; D019840:2-Propanol