Cross-species bacterial artificial chromosome-fluorescence in situ hybridization painting of the tomato and potato chromosome 6 reveals undescribed chromosomal rearrangements.
Ongoing genomics projects of tomato (Solanum lycopersicum) and potato (S. tuberosum) are providing unique tools for comparative mapping studies in Solanaceae. At the chromosomal level, bacterial artificial chromosomes (BACs) can be positioned on pachytene complements by fluorescence in situ hybridization (FISH) on homeologous chromosomes of related species. Here we present results of such a cross-species multicolor cytogenetic mapping of tomato BACs on potato chromosomes 6 and vice versa. The experiments were performed under low hybridization stringency, while blocking with Cot-100 was essential in suppressing excessive hybridization of repeat signals in both within-species FISH and cross-species FISH of tomato BACs. In the short arm we detected a large paracentric inversion that covers the whole euchromatin part with breakpoints close to the telomeric heterochromatin and at the border of the short arm pericentromere. The long arm BACs revealed no deviation in the colinearity between tomato and potato. Further comparison between tomato cultivars Cherry VFNT and Heinz 1706 revealed colinearity of the tested tomato BACs, whereas one of the six potato clones (RH98-856-18) showed minor putative rearrangements within the inversion. Our results present cross-species multicolor BAC-FISH as a unique tool for comparative genetic studies across Solanum species.
Tang
Xiaomin
X
Wageningen-UR Plant Breeding, PB Wageningen, The Netherlands.
Szinay
Dóra
D
Lang
Chunting
C
Ramanna
Munikote S
MS
van der Vossen
Edwin A G
EA
Datema
Erwin
E
Lankhorst
René Klein
RK
de Boer
Jan
J
Peters
Sander A
SA
Bachem
Christian
C
Stiekema
Willem
W
Visser
Richard G F
RG
de Jong
Hans
H
Bai
Yuling
Y
D002869:Chromosome Aberrations; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018551:Solanum lycopersicum / Q000235:genetics*; D020161:Physical Chromosome Mapping / Q000379:methods*; D011198:Solanum tuberosum / Q000235:genetics*; D013045:Species Specificity
D018744:DNA, Plant; D005819:Genetic Markers
Genetics. 1993 Dec;135(4):1175-86
7905845
Genetics. 1999 Jul;152(3):1183-202
10388833
Genetics. 2007 Aug;176(4):2131-8
17565940
Plant Cell. 1991 Jan;3(1):87-94
1668615
Genetics. 2000 Oct;156(2):833-8
11014828
Plant J. 2001 Dec;28(6):689-97
11851915
Plant J. 2008 Nov;56(4):627-37
18643986
Genetics. 2002 Aug;161(4):1697-711
12196412
Plant Mol Biol. 1993 Oct;23(1):185-92
8106010
Genetics. 2004 Dec;168(4):2127-40
15611181
Plant Physiol. 2006 Mar;140(3):805-17
16524981
Genetics. 1988 Dec;120(4):1095-103
17246486
J Struct Biol. 1991 Aug;107(1):6-14
1817611
Plant J. 2003 Sep;35(5):647-59
12940957
Chromosome Res. 1996 Jan;4(1):24-8
8653264
Chromosome Res. 2006;14(5):497-503
16823612
Genome Res. 2005 Apr;15(4):516-25
15781573
Chromosome Res. 2008;16(7):919-33
18688733
Genome. 2002 Dec;45(6):1003-12
12502244
Cytogenet Genome Res. 2006;114(3-4):222-6
16954657
Nature. 1991 Apr 18;350(6319):569-73
1708110
Cell. 2000 Feb 4;100(3):367-76
10676818
Annu Rev Phytopathol. 2001;39:285-312
11701867
Genetics. 2006 Jun;173(2):1075-87
16582432
Chromosome Res. 2002;10(3):223-32
12067211
Theor Appl Genet. 2004 Oct;109(6):1215-23
15340683
Trends Ecol Evol. 2004 Sep;19(9):482-8
16701311
Genetics. 2008 Nov;180(3):1307-17
18791232
Nat Protoc. 2006;1(2):783-90
17406308
Genetics. 1995 Oct;141(2):683-708
8647403
Theor Appl Genet. 2003 Feb;106(3):478-84
12589548
Genetics. 2000 Jun;155(2):873-87
10835406
Mol Gen Genet. 1996 Jul 19;251(5):526-31
8709958
Plant J. 2005 Oct;44(2):208-22
16212601
Genetics. 1992 Dec;132(4):1141-60
1360934
Genome. 1997 Feb;40(1):138-42
18464813
Cytogenet Genome Res. 2005;108(1-3):112-21
15545723
Plant Physiol. 2007 Oct;145(2):402-10
17720758
Chromosome Res. 2003;11(3):195-204
12769287
Chromosoma. 2004 Sep;113(2):77-83
15258808
Plant Physiol. 2001 Mar;125(3):1325-41
11244113
Plant Cell. 2002 Jul;14(7):1457-67
12119367
Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5224-9
16549785
Theor Appl Genet. 2004 May;108(8):1635-42
14963654
Plant Cell. 1998 Aug;10(8):1307-19
9707531
Theor Appl Genet. 1996 Jun;92(8):1065-72
24166637