title: The effect of low-level laser irradiation on dog spermatozoa motility is dependent on laser output power.
abstract: Biological tissues respond to low-level laser irradiation and so do dog spermatozoa. Among the main parameters to be considered when a biological tissue is irradiated is the output power. We have studied the effects on sperm motility of 655 nm continuous wave diode laser irradiation at different output powers with 3.34 J (5.97 J/cm(2)). The second fraction of fresh dog sperm was divided into five groups: control, and four to be irradiated with an average output power of 6.8 mW, 15.4 mW, 33.1 mW and 49.7 mW, respectively. At 0 min and 45 min after irradiation, pictures were taken and a computer aided sperm analysis (CASA) performed to analyse different motility parameters. The results showed that different output powers affected dog semen motility parameters differently. The highest output power showed the most intense effects. Significant changes in the structure of the motile sperm subpopulation were linked to the different output powers used.
authors.affiliation: Post-Degree Laser Medical Study, Rovira i Virgili University, Reus, Spain. marcignasi@yahoo.co.uk
authors.forename: M I
authors.initials: MI
authors.lastname: Corral-Baqués
authors.forename: M M
authors.initials: MM
authors.lastname: Rivera
authors.forename: T
authors.initials: T
authors.lastname: Rigau
authors.forename: J E
authors.initials: JE
authors.lastname: Rodríguez-Gil
authors.forename: J
authors.initials: J
authors.lastname: Rigau
mesh_terms: D000818:Animals; D004285:Dogs; D066298:In Vitro Techniques; D028022:Low-Level Light Therapy; D008297:Male; D055620:Optical Phenomena; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology / Q000528:radiation effects*; D013094:Spermatozoa / Q000145:classification / Q000502:physiology / Q000528:radiation effects
references.citation: Theriogenology. 1994;41(7):1355-66
references.pmid: 16727490
references.citation: J Photochem Photobiol B. 2005 Oct 3;81(1):26-32
references.pmid: 16107316
references.citation: J Reprod Fertil Suppl. 1993;47:261-70
references.pmid: 8229934
references.citation: Biol Reprod. 1999 Jan;60(1):32-41
references.pmid: 9858483
references.citation: Theriogenology. 2005 Sep 1;64(4):934-46
references.pmid: 16054497
references.citation: Photomed Laser Surg. 2007 Apr;25(2):102-6
references.pmid: 17508845
references.citation: Lasers Surg Med. 2007 Oct;39(9):706-15
references.pmid: 17960752
references.citation: Theriogenology. 2004 Feb;61(4):673-90
references.pmid: 14698057
references.citation: Lasers Surg Med. 2001;29(2):179-84
references.pmid: 11553908
references.citation: Reprod Domest Anim. 2006 Oct;41(5):408-15
references.pmid: 16984346
references.citation: J R Soc Interface. 2008 Mar 6;5(20):297-302
references.pmid: 17650470
references.citation: Lasers Med Sci. 2005;20(1):28-34
references.pmid: 15838719
references.citation: Fertil Steril. 2002 Aug;78(2):417-8
references.pmid: 12137884
references.citation: Anim Reprod Sci. 2005 Feb;85(3-4):317-25
references.pmid: 15581514
references.citation: Reprod Domest Anim. 2007 Jun;42(3):312-9
references.pmid: 17506811
references.citation: Photomed Laser Surg. 2006 Dec;24(6):705-14
references.pmid: 17199470
references.citation: Lasers Surg Med. 2004;34(3):277-84
references.pmid: 15022258
references.citation: Theriogenology. 1994 Oct;42(5):815-29
references.pmid: 16727587
references.citation: Photomed Laser Surg. 2005 Dec;23(6):549-55
references.pmid: 16356145
references.citation: Fertil Steril. 1994 Dec;62(6):1277-82
references.pmid: 7958000
references.citation: J Androl. 2000 Jul-Aug;21(4):515-24
references.pmid: 10901437