Characterization of a unique ClpB protein of Mycoplasma pneumoniae and its impact on growth.
Mycoplasma pneumoniae accounts for 20 to 30% of all community-acquired pneumonia and has been associated with other airway pathologies, including asthma, and a range of extrapulmonary manifestations. Although the entire genomic sequence of M. pneumoniae has been completed, the functions of many of these genes in mycoplasma physiology are unknown. In this study, we focused on clpB, a well-known heat shock gene in other bacteria, to examine its role in mycoplasma growth. Transcriptional and translational analyses of heat shock in M. pneumoniae indicated that clpB is significantly upregulated, reinforcing its status as a critical responder to heat stress. Interestingly, M. pneumoniae ClpB does not use dual translational start points for ClpB synthesis, like other ClpB-characterized bacteria. Biochemical characterization of purified M. pneumoniae recombinant ClpB revealed casein- and lysine-independent ATPase activity and DnaK-DnaJ-GrpE-dependent chaperone activity. An M. pneumoniae mini-Tn4001-integrated, clpB-null mutant was impaired in its ability to replicate under permissive growth conditions, demonstrating the growth-promoting status of ClpB.
Kannan
T R
TR
Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.
Musatovova
Oxana
O
Gowda
Pramod
P
Baseman
Joel B
JB
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D001483:Base Sequence; D015153:Blotting, Western; D029968:Escherichia coli Proteins / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins
Nucleic Acids Res. 2003 Nov 1;31(21):6306-20
14576319
FEMS Microbiol Lett. 2003 Dec 5;229(1):73-81
14659545
J Biol Chem. 2000 Dec 1;275(48):37565-71
10982797
Mol Microbiol. 1999 Jan;31(2):521-32
10027969
Mol Microbiol. 2002 Nov;46(4):1041-51
12421310
Subcell Biochem. 1993;20:243-59
8378992
Infect Immun. 2005 May;73(5):2828-34
15845487
Plasmid. 1993 Sep;30(2):170-5
8234492
J Bacteriol. 1991 Jul;173(14):4254-62
2066329
J Bacteriol. 2005 Jul;187(13):4542-51
15968065
J Bacteriol. 1996 Aug;178(16):4839-46
8759846
J Biol Chem. 1999 Oct 1;274(40):28083-6
10497158
J Biol Chem. 2002 Dec 6;277(49):47160-6
12351638
EMBO J. 1982;1(8):945-51
6329717
J Bacteriol. 1999 Apr;181(7):2075-83
10094684
Biochem Biophys Res Commun. 2008 Feb 29;367(1):213-8
18164681
EMBO J. 1997 Aug 1;16(15):4579-90
9303302
Int J Syst Bacteriol. 1997 Jan;47(1):38-45
8995799
Emerg Infect Dis. 1997 Jan-Mar;3(1):21-32
9126441
J Bacteriol. 2000 Jan;182(1):14-22
10613857
Nucleic Acids Res. 1996 Nov 15;24(22):4420-49
8948633
J Bacteriol. 1997 Aug;179(16):5111-7
9260953
Science. 1995 Oct 20;270(5235):397-403
7569993
FEMS Microbiol Lett. 2005 Dec 15;253(2):315-21
16260096
J Biol Chem. 2003 May 16;278(20):17615-24
12624113
EMBO J. 1999 Dec 15;18(24):6934-49
10601016
Proc Natl Acad Sci U S A. 1990 May;87(9):3513-7
2185473
Anal Biochem. 1979 Nov 15;100(1):95-7
161695
Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6724-9
16617115
J Mol Biol. 2002 Aug 2;321(1):111-20
12139937
Nat Struct Mol Biol. 2004 Jul;11(7):607-15
15208691
Mol Cell. 2007 Jan 26;25(2):247-60
17244532
J Bacteriol. 2006 Apr;188(8):2845-55
16585746
Clin Microbiol Rev. 2004 Oct;17(4):697-728, table of contents
15489344
J Bacteriol. 1993 Mar;175(6):1637-44
8449872
J Bacteriol. 1998 Aug;180(15):3873-81
9683484
J Biol Chem. 1992 Oct 5;267(28):20429-34
1400361
Biochem Soc Trans. 2008 Feb;36(Pt 1):120-5
18208398
Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7184-9
10377389
Anal Biochem. 1975 Feb;63(2):607-13
1122033
FEMS Immunol Med Microbiol. 1996 Oct;15(4):199-211
8908481
Nucleic Acids Res. 2000 Nov 15;28(22):4488-96
11071937
J Bacteriol. 1994 Mar;176(5):1359-63
8113175