title: Rapid chemiluminometric determination of gabapentin in pharmaceutical formulations exploiting pulsed-flow analysis.
abstract: In this study, a straightforward and automated pulsed flow-based procedure was developed for the chemiluminometric determination of gabapentin [1-(aminomethyl)cyclo-hexaneacetic acid], a new generation antiepileptic drug, in different formulated dosage forms. The software-controlled time-based injection method capitalizes on the decrease of the background chemiluminescence (CL) readout of the luminol-hypochlorite reaction in the presence of gabapentin. In short, gabapentin works as a hypochlorite scavenger. The analytical procedure was implemented in a multi-pumping flow network furnished with a suite of microdispensing solenoid-actuated pumps. The diaphragm-type micropumps might be configured to operate as fluid propellers, commutation units and metering injectors. A dynamic linear working range for gabapentin concentrations in the range 60-350 micromol/L was obtained, with an estimated detection limit of 40 micromol/L. The flow analyser handles about 41 injections/h and yields precise results (RSD < 2%). The miniaturized flow analyser thus has potential to be exploited for in-line monitoring of drug manufacturing within the quality assurance framework of modern pharmaceutical companies.
authors.affiliation: Department of Chemistry, University of the Balearic Islands, Carretera de Valldemossa km. 7.5, E-07122 Palma de Mallorca, Spain.
authors.forename: Matías
authors.initials: M
authors.lastname: Manera
authors.forename: Manuel
authors.initials: M
authors.lastname: Miró
authors.forename: Marta F T
authors.initials: MF
authors.lastname: Ribeiro
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Estela
authors.forename: Víctor
authors.initials: V
authors.lastname: Cerdà
authors.forename: João L M
authors.initials: JL
authors.lastname: Santos
authors.forename: José L F C
authors.initials: JL
authors.lastname: Lima
mesh_terms: D000588:Amines / Q000032:analysis* / Q000737:chemistry*; D002138:Calibration; D003509:Cyclohexanecarboxylic Acids / Q000032:analysis* / Q000737:chemistry*; D000077206:Gabapentin; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000737:chemistry*
chemical_list: D000588:Amines; D003509:Cyclohexanecarboxylic Acids; D004364:Pharmaceutical Preparations; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin