Identification of coagulase-negative Staphylococci by using the BD phoenix system in the low-inoculum mode.
The new "low-inoculum" mode of the Phoenix system was evaluated to identify clinical coagulase-negative staphylococci. API ID32 Staph panels were used as comparators, and discrepancies were resolved by 16S rRNA and tuf gene analysis. The system correctly identified 90.5% of isolates, with a mean time of 10.2 h. Accuracy was satisfactory for Staphylococcus epidermidis, S. saprophyticus, and S. haemolyticus.
Brigante
Gioconda
G
Department of Microbiology, University of Catania, Via Androne 81, 95124 Catania, Italy.
Menozzi
Maria Grazia
MG
Pini
Beatrice
B
Porta
Rosaria
R
Somenzi
Pietro
P
Sciacca
Agata
A
Spanu
Teresa
T
Stefani
Stefania
S
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D003030:Coagulase / Q000096:biosynthesis*; D006801:Humans; D010444:Peptide Elongation Factor Tu / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification*
D001426:Bacterial Proteins; D003030:Coagulase; D012336:RNA, Ribosomal, 16S; D010444:Peptide Elongation Factor Tu
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