In vitro selection of a DNAzyme with three modified nucleotides.
Three modified nucleosides have been used in the in vitro selection of the self-cleaving DNAzyme 9-33. This DNAzyme operates in the absence of divalent metal cations with a first-order constant of approximately 0.05 min(-1).
Hollenstein
Marcel
M
Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver BC, V6T 1Z1, Canada.
Hipolito
Christopher
C
Lam
Curtis
C
Perrin
David
D
D001483:Base Sequence; D002384:Catalysis; D021881:DNA, Catalytic / Q000737:chemistry*; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000737:chemistry*
D021881:DNA, Catalytic; C541612:DNAzyme 9-33; D009711:Nucleotides