Caged neuron MEA: a system for long-term investigation of cultured neural network connectivity.
Traditional techniques for investigating cultured neural networks, such as the patch clamp and multi-electrode array, are limited by: (1) the number of identified cells which can be simultaneously electrically contacted, (2) the length of time for which cells can be studied, and (3) the lack of one-to-one neuron-to-electrode specificity. Here, we present a new device - the caged neuron multi-electrode array - which overcomes these limitations. This micro-machined device consists of an array of neurocages which mechanically trap a neuron near an extracellular electrode. While the cell body is trapped, the axon and dendrites can freely grow into the surrounding area to form a network. The electrode is bi-directional, capable of both stimulating and recording action potentials. This system is non-invasive, so that all constituent neurons of a network can be studied over its lifetime with stable one-to-one neuron-to-electrode correspondence. Proof-of-concept experiments are described to illustrate that functional networks form in a neurochip system of 16 cages in a 4 x 4 array, and that suprathreshold connectivity can be fully mapped over several weeks. The neurochip opens a new domain in neurobiology for studying small cultured neural networks.
Erickson
Jonathan
J
Department of Bioengineering, California Institute of Technology, Pasadena, CA 91125, USA. jonathan.erickson@vanderbilt.edu
Tooker
Angela
A
Tai
Y-C
YC
Pine
Jerome
J
D000200:Action Potentials / Q000502:physiology / Q000528:radiation effects; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002470:Cell Survival; D002478:Cells, Cultured / Q000502:physiology; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D004622:Embryo, Mammalian; D006624:Hippocampus / Q000166:cytology; D008838:Microcomputers; D008855:Microscopy, Electron, Scanning; D008959:Models, Neurological; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D051381:Rats; D012825:Silicon
D012825:Silicon
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