title: DNA methylation changes in cell line from beta-lactoglobulin gene targeted fetus.
abstract: The gene targeting combined somatic cell nuclear transfer is very useful in agriculture and medicine. Epigenetic modification of DNA by methylation is significant in regulating gene expression during mammalian development. During gene targeting, epigenetic status of donor cell nuclei may be changed in a series of processes, including homologous recombination, cell selection and cloning. We examined DNA methylation of six genes (beta-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence art2 in blg(+/-) cell line from beta-lactoglobulin (BLG) gene targeted fetus and the cells used for BLG gene targeting serve as control. The results demonstrated that the widespread changes of DNA methylation were found in blg(+/-) cell line. But the degree of variation was different. DNA methylation of VEGF in blg(+/-) was noticeably decreased. These observations suggest that DNA methylation variations may impact gene expression and finally induce abnormalities and lethality in later developmental stages.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Yuanmingyuan West Road 2, Beijing 100094, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Weizhuo
authors.initials: W
authors.lastname: Xu
authors.forename: Yunping
authors.initials: Y
authors.lastname: Dai
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D002417:Cattle; D002460:Cell Line; D018899:CpG Islands; D019175:DNA Methylation / Q000502:physiology; D005333:Fetus / Q000166:cytology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting / Q000379:methods / Q000662:veterinary; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D007782:Lactoglobulins / Q000235:genetics* / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics; D019098:Telomerase / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: C094335:H19 long non-coding RNA; D007782:Lactoglobulins; D050814:Octamer Transcription Factor-3; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D042461:Vascular Endothelial Growth Factor A; D007335:Insulin-Like Growth Factor II; D036002:ADP Ribose Transferases; D019098:Telomerase