title: Anandamide effects on 5-HT(3) receptors in vivo.
abstract: Studies in knockout mouse strains have shown that some cannabimimetic effects persist in animals lacking cannabinoid CB(1) and CB(2) receptors. These residual effects are thought to result, in part, from a cannabinoid-modulation of ion channels. This study investigates the role of 5-HT(3) receptors as a potential in vivo target for cannabinoids. Mice deficient in CB(1) and CB(2) receptors were treated with Delta(9)-tetrahydrocannabinol and anandamide, in the presence of the 5-HT(3) antagonist ondansetron. We show that the cannabinoid receptor-independent anandamide analgesia, but not catalepsy, is completely blocked by ondansetron. Thus, 5-HT(3) receptors seem to be involved in cannabinoid analgesia.
authors.affiliation: Institute of Molecular Psychiatry, Life & Brain Center, University of Bonn, Sigmund-Freud-Strasse 25, Bonn, Germany.
authors.forename: Ildikó
authors.initials: I
authors.lastname: Rácz
authors.forename: Andras
authors.initials: A
authors.lastname: Bilkei-Gorzo
authors.forename: Astrid
authors.initials: A
authors.lastname: Markert
authors.forename: Friederike
authors.initials: F
authors.lastname: Stamer
authors.forename: Manfred
authors.initials: M
authors.lastname: Göthert
authors.forename: Andreas
authors.initials: A
authors.lastname: Zimmer
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D013759:Dronabinol / Q000494:pharmacology; D063388:Endocannabinoids; D046352:Freezing Reaction, Cataleptic / Q000187:drug effects; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009043:Motor Activity / Q000187:drug effects; D017294:Ondansetron / Q000494:pharmacology; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D043884:Receptor, Cannabinoid, CB1 / Q000235:genetics*; D043885:Receptor, Cannabinoid, CB2 / Q000235:genetics*; D044406:Receptors, Serotonin, 5-HT3 / Q000502:physiology*; D058831:Serotonin 5-HT3 Receptor Antagonists
chemical_list: D000701:Analgesics, Opioid; D001095:Arachidonic Acids; D063388:Endocannabinoids; D053284:Polyunsaturated Alkamides; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D044406:Receptors, Serotonin, 5-HT3; D058831:Serotonin 5-HT3 Receptor Antagonists; D017294:Ondansetron; D013759:Dronabinol; C078814:anandamide