Incorporation of cationic dyes into DNA for distinct stabilization of duplex.
In this study, cationic dyes (methylstilbazole) were introduced into ODN. When two complementary ODNs, both of which tethered this dye, were hybridized, the melting temperature drastically increased. The duplex was further stabilized by introducing multiple dyes.
Kashida
Hiromu
H
Graduate School of Engineering, Nagoya University, Furocho, Chikusa, Nagoya 464-8603, Japan.
Itoh
Hidehiro
H
Fujii
Taiga
T
Asanuma
Hiroyuki
H
D000605:Amino Alcohols / Q000737:chemistry; D002072:Butylene Glycols / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000737:chemistry*; D013057:Spectrum Analysis; D013343:Styrenes / Q000737:chemistry*
D000605:Amino Alcohols; D002072:Butylene Glycols; D002412:Cations; D004396:Coloring Agents; D009838:Oligodeoxyribonucleotides; D011726:Pyridinium Compounds; D013343:Styrenes; C532652:threoninol; D004247:DNA