title: Fluorescence nanoscopy by ground-state depletion and single-molecule return.
abstract: We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Jonas
authors.initials: J
authors.lastname: Fölling
authors.forename: Mariano
authors.initials: M
authors.lastname: Bossi
authors.forename: Hannes
authors.initials: H
authors.lastname: Bock
authors.forename: Rebecca
authors.initials: R
authors.lastname: Medda
authors.forename: Christian A
authors.initials: CA
authors.lastname: Wurm
authors.forename: Birka
authors.initials: B
authors.lastname: Hein
authors.forename: Stefan
authors.initials: S
authors.lastname: Jakobs
authors.forename: Christian
authors.initials: C
authors.lastname: Eggeling
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*