D-Amino acids in rat brain measured by liquid chromatography/tandem mass spectrometry.
Previous work has established that D-amino acids including D-serine (D-Ser) and D-aspartic acid (D-Asp) fulfill specific biological functions in the brain. In this work, the levels and anatomical distribution of d-amino acids in rat brain were determined by using an advantageous liquid chromatography/tandem mass spectrometric analytical method. The study was focused on D-Ser, D-Asp, and D-glutamic acid (D-Glu) because of the significance of L-Asp, L-Glu, and D-Ser in the nervous system. Prenatal, postnatal pups, and 90-day old rats were studied. Results indicated that D-Asp and D-Ser occurred in rat brain at the microg/g tissue level. However, D-Glu was not detected (< 110 ng/g tissue). Throughout the developmental stages d-Asp content in rat brain decreased rapidly from 9.42% of total Asp in 5-day prenatal rats to an undetectable level (< 150 ng/g tissue) in 90-day old rats. In contrast, D-Ser level increased gradually throughout the developmental stages. D-Ser percentage (D-Ser/(D-Ser + L-Ser)) changed from 4.94% in 5-day prenatal rats to 13.7% in 90-day old rats. Regional levels of D-Ser were found to be significantly higher in cortex, striatum, and hippocampus than in thalamus. D-Ser was not detected in cerebellum (< 172ng/g tissue).
Song
Yaru
Y
Department of Chemistry and Biochemistry, Jackson State University, 1400 Lynch St., Jackson, MS 39217, USA.
Feng
Yangzheng
Y
Lu
Xin
X
Zhao
Shulin
S
Liu
Cheng-Wei
CW
Liu
Yi-Ming
YM
D000367:Age Factors; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004622:Embryo, Mammalian; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000596:Amino Acids
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