title: Identification of yak lactate dehydrogenase B gene variants by gene cloning.
abstract: Native polyacrylamide gel electrophoresis showed that two types of lactate dehydrogenase (LDH) existed in yaks. Based on the electrophoresis characteristics of LDH isoenzymes, yak LDH variants were speculated to be the gene mutation on H subunit encoded by B gene. According to the mobility in electrophoresis, the fast-band LDH type was named LDH-Hf and the slow-band LDH type LDH-Hs. In order to reveal the gene alteration in yak LDH variants, total RNA was extracted from heart tissues of yaks with different LDH variants, and cDNAs of the two variants were reverse transcripted. Two variants of B genes were cloned by RT-PCR. Sequence analysis revealed that four nucleotides differed between LDH-Bf and LDH-Bs, which resulted in two amino acids alteration. By Deepview software analysis of the conformation of yak LDH1 variants and H subunit, these four nucleotides altered two amino acids that generated new hydrogen bonds to change the hydrogen bonds network, and further caused subtle conformational changes between the two LDH variants.
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China.
authors.forename: YuCai
authors.initials: Y
authors.lastname: Zheng
authors.forename: XingBo
authors.initials: X
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Piao
authors.forename: SuYu
authors.initials: S
authors.lastname: Jin
authors.forename: QingHua
authors.initials: Q
authors.lastname: He
authors.forename: Jian
authors.initials: J
authors.lastname: Hong
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: ChangXin
authors.initials: C
authors.lastname: Wu
mesh_terms: D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007527:Isoenzymes / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008958:Models, Molecular; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; C409667:lactate dehydrogenase 1