title: Transcriptomic analysis of the exit from dormancy of Aspergillus fumigatus conidia.
abstract: BACKGROUND
  Establishment of aspergillosis is depending upon the exit from dormancy and germination of the conidia of Aspergillus fumigatus in the lung. To gain an understanding of the molecular mechanisms underlying the early steps of conidial germination, we undertook a transcriptomic analysis using macroarrays constructed with PCR fragments from > 3,000 genes (around one third of the annotated A. fumigatus genome).
  
  
  RESULTS
  Major results of this analysis are the following: (i) conidia stored pre-packaged mRNAs transcripts (27% of genes have transcripts in the resting conidia; (ii) incubation at 37 degrees C in a nutritive medium induced up- and down-regulation of genes: 19% of the total number of genes deposited on the array were up-regulated whereas 22% of the genes with pre-packaged mRNA in the resting conidia were down-regulated; (iii) most modifications were seen during the first 30 min of germination whereas very little modification of gene expression occurred during the following hour; (iv) one-year old conidia and one-week old conidia behaved similarly at transcriptional level.
  
  
  CONCLUSIONS
  Transcriptomic data indicate that the exit from dormancy is associated with a shift from a fermentative metabolism to a respiratory metabolism as well as a trend toward immediate protein synthesis.
authors.affiliation: Unité des Aspergillus, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France. mechant_boris@hotmail.com
authors.forename: Claude
authors.initials: C
authors.lastname: Lamarre
authors.forename: Sergueï
authors.initials: S
authors.lastname: Sokol
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Debeaupuis
authors.forename: Christine
authors.initials: C
authors.lastname: Henry
authors.forename: Céline
authors.initials: C
authors.lastname: Lacroix
authors.forename: Philippe
authors.initials: P
authors.lastname: Glaser
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Coppée
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: François
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Latgé
mesh_terms: D001232:Aspergillus fumigatus / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger
references.citation: Genes Chromosomes Cancer. 2006 Jan;45(1):1-10
references.pmid: 16175572
references.citation: Mol Genet Genomics. 2005 Jun;273(5):394-403
references.pmid: 15887033
references.citation: Cell. 1980 Oct;21(3):709-15
references.pmid: 6449291
references.citation: Med Mycol. 2001 Apr;39(2):195-8
references.pmid: 11346268
references.citation: Fungal Genet Biol. 2008 Mar;45(3):232-42
references.pmid: 17703972
references.citation: Biochemistry. 2006 Feb 14;45(6):1979-86
references.pmid: 16460045
references.citation: Eukaryot Cell. 2006 Nov;5(11):1934-40
references.pmid: 16998074
references.citation: Fungal Genet Biol. 2004 Feb;41(2):199-212
references.pmid: 14732266
references.citation: Diagn Microbiol Infect Dis. 2006 Aug;55(4):279-91
references.pmid: 16626916
references.citation: Fungal Genet Biol. 2005 Jul;42(7):638-45
references.pmid: 15896988
references.citation: Infect Immun. 2004 Sep;72(9):5193-203
references.pmid: 15322014
references.citation: J Biol Chem. 2007 Apr 13;282(15):11078-83
references.pmid: 17303556
references.citation: Mol Biol Cell. 2004 Dec;15(12):5295-305
references.pmid: 15456898
references.citation: Fungal Genet Biol. 2004 Mar;41(3):317-26
references.pmid: 14761792
references.citation: Cell. 2003 Oct 17;115(2):135-8
references.pmid: 14567911
references.citation: Eukaryot Cell. 2004 Apr;3(2):557-60
references.pmid: 15075285
references.citation: BMC Evol Biol. 2006 Dec 19;6:109
references.pmid: 17178000
references.citation: Appl Environ Microbiol. 2006 Feb;72(2):1667-71
references.pmid: 16461724
references.citation: Dev Biol. 1980 Aug;78(2):497-510
references.pmid: 6997115
references.citation: Nucleic Acids Res. 2005 Nov 14;33(20):6469-85
references.pmid: 16287898
references.citation: Eukaryot Cell. 2002 Feb;1(1):33-43
references.pmid: 12455969
references.citation: Microbiology (Reading). 1999 Oct;145 ( Pt 10):2625-34
references.pmid: 10537184
references.citation: BMC Genomics. 2007 Apr 11;8:100
references.pmid: 17428342
references.citation: Genetics. 2000 Jun;155(2):647-56
references.pmid: 10835388
references.citation: Microbiol Mol Biol Rev. 2004 Jun;68(2):187-206
references.pmid: 15187181
references.citation: Nature. 2005 Dec 22;438(7071):1151-6
references.pmid: 16372009
references.citation: Curr Genet. 2006 Jul;50(1):32-44
references.pmid: 16622700
references.citation: Annu Rev Physiol. 2007;69:145-70
references.pmid: 17037980
references.citation: J Biol Chem. 2002 Oct 25;277(43):40505-12
references.pmid: 12181322
references.citation: Fungal Genet Biol. 2004 Apr;41(4):391-400
references.pmid: 14998522
references.citation: Mol Biol Cell. 2005 Dec;16(12):5866-79
references.pmid: 16207816
references.citation: FEMS Microbiol Lett. 2001 May 30;199(2):153-60
references.pmid: 11377860
references.citation: FEMS Yeast Res. 2005 Dec;5(12):1253-9
references.pmid: 16099222
references.citation: Mol Microbiol. 2003 Mar;47(6):1613-25
references.pmid: 12622816
references.citation: Antimicrob Agents Chemother. 2002 Mar;46(3):615-24
references.pmid: 11850239
references.citation: Mol Biol Cell. 1994 Oct;5(10):1065-80
references.pmid: 7865875
references.citation: Mol Microbiol. 1999 May;32(3):471-83
references.pmid: 10320571
references.citation: Microbiol Mol Biol Rev. 1998 Mar;62(1):35-54
references.pmid: 9529886
references.citation: Fungal Genet Biol. 2002 Nov;37(2):197-204
references.pmid: 12409104
references.citation: Trends Plant Sci. 2007 May;12(5):217-23
references.pmid: 17416545
references.citation: Eukaryot Cell. 2004 Oct;3(5):1101-10
references.pmid: 15470238
references.citation: Mol Cell Biol. 2002 Oct;22(20):6993-7003
references.pmid: 12242280
references.citation: Appl Microbiol Biotechnol. 1998 Aug;50(2):193-8
references.pmid: 9763690
references.citation: Biochemistry. 2005 Jan 25;44(3):893-904
references.pmid: 15654745
references.citation: Plant Physiol. 2001 Jan;125(1):180-8
references.pmid: 11154327
references.citation: Nucleic Acids Res. 1996 Apr 15;24(8):1428-34
references.pmid: 8628674
references.citation: Mol Biol Cell. 1998 Oct;9(10):2963-71
references.pmid: 9763455
references.citation: Infect Immun. 2006 Aug;74(8):4865-74
references.pmid: 16861675
references.citation: J Biol. 2007;6(2):4
references.pmid: 17439666
references.citation: PLoS Pathog. 2007 Apr;3(4):e50
references.pmid: 17432932