Northern analysis of viral plus- and minus-strand RNAs.
Replication is a fundamental activity of viruses. Replication of positive-sense RNA viruses involves the synthesis of complementary minus-strand intermediates from the parental RNA template followed by synthesis of nascent plus strands. Negative-sense RNA genome and double-stranded RNA are copied into positive-sense mRNA before translation. To detect and estimate the abundance of plus- and minus-strand viral transcripts in the infected samples, northern analysis is the most commonly used method.
Palani
Paramasivan Vijaya
PV
Institute of Plant and Microbial Biology, Taipei, Taiwan.
Lin
Na-Sheng
NS
D015152:Blotting, Northern / Q000379:methods*; D010935:Plant Diseases / Q000821:virology; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014026:Nicotiana / Q000821:virology; D014779:Virus Replication
D012330:RNA, Double-Stranded; D012367:RNA, Viral