title: The effect of neuronal expression of heat shock proteins 26 and 27 on lifespan, neurodegeneration, and apoptosis in Drosophila.
abstract: Heat shock proteins (Hsps) are chaperones thought to increase lifespan, enhance stress resistance, and prevent apoptosis and neurodegenerative diseases. Our previous study reported that ubiquitous expression of hsp26 or hsp27 extended Drosophila lifespan. The effect of neuronal expression of hsp26 and hsp27 in Drosophila on the above-mentioned functions has not yet been investigated. Here, we show that neuronal expression of hsp26 or hsp27 improved lifespan and increased resistance to oxidative stress. However, only neuronal expression of hsp27 ameliorated Parkinsonism climbing disorder and attenuated mild polyglutamine-induced toxicity. Additionally, neuronal expression of hsp27 specifically partially rescued hid-induced lethality, but was not able to rescue reaper/grim-induced lethality. However, unlike hsp27, neuronal expression of hsp26 did not rescue hid-induced or reaper/grim-induced lethality. In summary, we demonstrate the functional similarities and differences of neuronal expression of hsp26 and hsp27 in adult Drosophila.
authors.affiliation: Department of Life Science and Institute of Biotechnology, National Tsing Hua University, HsinChu 30013, Taiwan, ROC.
authors.forename: Pin-Chao
authors.initials: PC
authors.lastname: Liao
authors.forename: Hung-Yu
authors.initials: HY
authors.lastname: Lin
authors.forename: Chiou-Hwa
authors.initials: CH
authors.lastname: Yuh
authors.forename: Lin-Kwei
authors.initials: LK
authors.lastname: Yu
authors.forename: Horng-Dar
authors.initials: HD
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D004195:Disease Models, Animal; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005260:Female; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008136:Longevity / Q000235:genetics; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress; D020734:Parkinsonian Disorders / Q000378:metabolism*; D010455:Peptides / Q000633:toxicity
chemical_list: D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; C087442:Hsp26 protein, Drosophila; D010455:Peptides; C097188:polyglutamine