Magnetic field affects enzymatic ATP synthesis.
The rate of ATP synthesis by creatine kinase extracted from V. xanthia venom was shown to depend on the magnetic field. The yield of ATP produced by enzymes with 24Mg2+ and 26Mg2+ ions in catalytic sites increases by 7-8% at 55 mT and then decreases at 80 mT. For enzyme with 25Mg2+ ion in a catalytic site, the ATP yield increases by 50% and 70% in the fields 55 and 80 mT, respectively. In the Earth field the rate of ATP synthesis by enzyme, in which Mg2+ ion has magnetic nucleus 25Mg, is 2.5 times higher than that by enzymes, in which Mg2+ ion has nonmagnetic, spinless nuclei 24Mg or 26Mg. Both magnetic field effect and magnetic isotope effect demonstrate that the ATP synthesis is an ion-radical process, affected by Zeeman interaction and hyperfine coupling in the intermediate ion-radical pair.
Buchachenko
Anatoly L
AL
N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow 119991, Russia. abuchach@chph.ras.ru
Kuznetsov
Dmitry A
DA
D000255:Adenosine Triphosphate / Q000096:biosynthesis* / Q000737:chemistry*; D000818:Animals; D002384:Catalysis; D020134:Catalytic Domain; D003402:Creatine Kinase / Q000737:chemistry* / Q000378:metabolism; D007554:Isotopes; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry*; D008280:Magnetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D010766:Phosphorylation; D014757:Viper Venoms / Q000201:enzymology; D017819:Viperidae
D007554:Isotopes; D014757:Viper Venoms; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D008274:Magnesium