Impaired growth of small intestinal epithelium by adrenalectomy in weaning rats.
Functional maturation of the small intestine occurs during the weaning period in rats. It is known that this development is facilitated by glucocorticoid. However, the effect of glucocorticoid on morphological development of small intestine has yet to be clarified. The present study evaluated the morphological development and cell proliferation of the small intestine in adrenalectomized (ADX) rat pups. To further understand the mechanism of glucocorticoid effects on intestinal development, we examined the localization of the glucocorticoid receptor in the small intestine. Microscopic analysis showed that growth of villi and crypts is age-dependent, and is significantly attenuated in ADX rats compared with sham-operated rats. BrdU-positive cells, i.e. proliferating cells, were primarily observed in crypt compartments and rapidly increased in number during the early weaning period. The increase in BrdU-positive cells could be attenuated by adrenalectomy. The morphological development of small intestine may be associated with increased proliferation of epithelial cells. On the other hand, glucocorticoid receptors were found in epithelial cells of the mid- and lower villi and not in crypts where BrdU-positive cells were localized. These results indicate that the growth of small intestine is attenuated by adrenalectomy, and that glucocorticoid indirectly acts on proliferation of epithelial cells during the weaning period.
Miyata
Tohru
T
Department of Bioresource Science, College of Agriculture, Tamagawa University, 6-1-1 Tamagawa-gakuen, Machida, Tokyo 194-8610, Japan. mytto4ac@agr.tamagawa.ac.jp
Minai
Yuji
Y
Haga
Minoru
M
J Clin Pathol. 1981 Oct;34(10):1181-6
7309898
Reprod Fertil Dev. 1996;8(1):35-48
8713721
Am J Physiol. 1984 Jun;246(6 Pt 1):G695-9
6742120
Cell Tissue Kinet. 1985 May;18(3):255-62
3986870
Pediatr Res. 1992 Sep;32(3):306-11
1408467
Anat Rec. 1981 Apr;199(4):565-74
7270915
Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):266-71
14695885
Mol Cell Biol. 1997 Jun;17(6):3181-93
9154817
Am J Physiol Gastrointest Liver Physiol. 2005 May;288(5):G897-906
15826934
Pediatr Res. 2001 Jun;49(6):782-8
11385138
J Anal Toxicol. 1985 Jan-Feb;9(1):20-3
3920444
Am J Physiol Gastrointest Liver Physiol. 2004 Jun;286(6):G947-53
14739144
Biochem Biophys Res Commun. 1998 May 29;246(3):765-70
9618286
J Anat. 1972 May;112(Pt 1):27-33
5086211
Mol Cell Biol. 1988 Apr;8(4):1449-59
3380086
Am J Physiol Gastrointest Liver Physiol. 2005 Sep;289(3):G381-7
16093419
Gut. 1996 Oct;39(4):532-8
8944561
Dig Dis Sci. 2006 Apr;51(4):718-23
16614994
Genes Dev. 2003 Jul 15;17(14):1709-13
12865297
Biochim Biophys Acta. 1964 Nov 22;92:305-11
14249119
Gut. 1995 Jun;36(6):864-73
7615275
Am J Physiol. 1993 Feb;264(2 Pt 1):G306-11
8447413
Histochemistry. 1988;89(4):351-6
3261722
J Histochem Cytochem. 1999 Jul;47(7):949-58
10375383
Ann N Y Acad Sci. 1995 Jun 12;761:261-75
7625725
Gut. 2002 Nov;51(5):748-54
12377819