Combination DNA/RNA FISH and immunophenotyping.
This unit presents methods for combining immunophenotyping with DNA/RNA FISH. The approach is used in so-called genotype/phenotype analysis to identify chromosomal aberrations in sub-populations of cells present in heterogenous populations. Combining RNA and DNA detection with identification of cellular proteins is quite difficult. This series of protocols is provided to enable the successful application of the combination of these techniques.
Dirks
R W
RW
Leiden University Medical Centre, Leiden, The Netherlands.
D000818:Animals; D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis; D005838:Genotype; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D010641:Phenotype; D012313:RNA / Q000032:analysis*
D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA