Seeding osteoblastic cells into a macroporous biodegradable CaP/PLGA scaffold by a centrifugal force.
This study aims to construct a hybrid biomaterial by seeding osteoblastic cells into a CaP/PLGA scaffold by a centrifugal force. Constructs are evaluated with respect to potential application in bone tissue engineering. Cells adher, spread, and form a layer of tissue lining the scaffold and are capable of migrating, proliferating, and producing mineralized matrix. We have demonstrated that the centrifugal force is highly efficient for constructing a hybrid biomaterial, which acts similarly to bone explants in a cell culture environment. In this way, these constructs could mimic an autogenous bone graft in clinical circumstances. Such a strategy may be useful for bone tissue engineering.
Beloti
Marcio Mateus
MM
University of Sao Paulo, SP, Brazil.
Tambasco De Oliveira
Paulo
P
Perri De Carvalho
Paulo Sergio
PS
Rosa
Adalberto Luiz
AL
D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured; D002498:Centrifugation / Q000379:methods; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid