title: Clonal relationship among Vibrio cholerae O1 El Tor strains isolated in Somalia.
abstract: One hundred and three Vibrio cholerae O1 strains, selected to represent the cholera outbreaks which occurred in Somalia in 1998-1999, were characterized by random amplified polymorphic DNA patterns, ribotyping, and antimicrobial susceptibility. All strains showed a unique amplified DNA pattern and 2 closely related ribotypes (B5a and B8a), among which B5a was the more frequently identified. Ninety-one strains were resistant to ampicillin, chloramphenicol, spectinomycin, streptomycin, sulfamethoxazole, and trimethoprim, conferred, except for spectinomycin, by a conjugative plasmid IncC. These findings indicated that the group of strains active in Somalia in the late 1990s had a clonal origin.
authors.affiliation: Dipartimento di Genetica e Microbiologia, Università di Bari, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Scrascia
authors.forename: Nicola
authors.initials: N
authors.lastname: Pugliese
authors.forename: Francesco
authors.initials: F
authors.lastname: Maimone
authors.forename: Kadigia A
authors.initials: KA
authors.lastname: Mohamud
authors.forename: Patrick A D
authors.initials: PA
authors.lastname: Grimont
authors.forename: Sadiki F
authors.initials: SF
authors.lastname: Materu
authors.forename: Carlo
authors.initials: C
authors.lastname: Pazzani
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D016000:Cluster Analysis; D003227:Conjugation, Genetic; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids; D019105:Random Amplified Polymorphic DNA Technique; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D012998:Somalia / Q000453:epidemiology; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial