title: Regulation of kinin B(2) receptors by bradykinin in human lung cells.
abstract: Bradykinin is a potent mediator of inflammation that has been shown to participate in allergic airway inflammation. The biologic effects of bradykinin are mediated by binding and activation of its cognate receptor, the B(2) receptor (B(2)R). In the lung fibroblast cell line IMR-90, binding of bradykinin to B(2)R triggers down-regulation of receptor surface expression, suggesting that bradykinin-induced inflammation is transient and self-limited. Notably, subjects with chronic airway inflammation continue to respond to BK following a first challenge. B(2)Rs are expressed on many different lung cell types, including airway epithelial cells. We therefore compared IMR-90 cells with the human lung epithelial cell line BEAS2B and found that B(2)R expression in the two cell types is differently regulated by BK. Whereas BK induces down-regulation of B(2)R in IMR-90 cells, the same treatment leads to up-regulation of the receptor in BEAS2B cells. These results provide a possible explanation for the potency of bradykinin in inducing ongoing airway inflammation.
authors.affiliation: Department of Clinical Sciences, Lund University, S-22184 Lund, Sweden. sara.mattsson@med.lu.se
authors.forename: Sara H
authors.initials: SH
authors.lastname: Bengtson
authors.forename: Jane
authors.initials: J
authors.lastname: Eddleston
authors.forename: Matthias
authors.initials: M
authors.lastname: Mörgelin
authors.forename: Bruce L
authors.initials: BL
authors.lastname: Zuraw
authors.forename: Heiko
authors.initials: H
authors.lastname: Herwald
mesh_terms: D001920:Bradykinin / Q000494:pharmacology*; D002460:Cell Line; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000187:drug effects*; D011485:Protein Binding / Q000187:drug effects; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043782:Receptor, Bradykinin B2 / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D043782:Receptor, Bradykinin B2; D001920:Bradykinin