title: WU and KI polyomavirus present in the respiratory tract of children, but not in immunocompetent adults.
abstract: BACKGROUND
  Recently, two new polyomaviruses (PyV), termed WUPyV and KIPyV, were identified in respiratory tract specimens from children with acute respiratory tract infections (ARTIs). However, their roles in the disease have not been determined.
  
  
  OBJECTIVE
  To determine the prevalence of WUPyV and KIPyV in the Chinese population suffering from ARTIs in Beijing, China, and to examine their possible role in causing disease.
  
  
  METHODS
  Nasopharyngeal aspirates, nasal swabs and throat swabs were collected from 415 children and 297 immunocompetent adults with lower ARTIs (LARTIs). The specimens were screened by polymerase chain reaction for the presence of WUPyV, KIPyV, and other common respiratory pathogens.
  
  
  RESULTS
  Although none of the adults sampled were positive for either virus, WUPyV in 10 (2.4%) children and KIPyV was detected in 2 (0.5%) of the children sampled, respectively. Eleven of the positive cases were co-detected with either rhinovirus (6/11), respiratory syncytial virus (4/11), parainfluenzavirus virus (3/11) or Mycoplasma pneumoniae (2/11). Phylogenetic analysis of the WUPyV and KIPyV isolates showed that the nucleotide sequences were homologous to those of previously reported strains.
  
  
  CONCLUSIONS
  The presence of WUPyV and KIPyV in samples from children but not from immunocompetent adults suffering from LARTIs suggests that these viruses primarily infect the young population. Co-detection of additional respiratory pathogens in most of the specimens containing either WUPyV or KIPyV suggests that these viruses do not cause disease independently.
authors.affiliation: Dr. Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux and State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS), Beijing 100730, P R China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ren
authors.forename: Richard
authors.initials: R
authors.lastname: Gonzalez
authors.forename: Zhengde
authors.initials: Z
authors.lastname: Xie
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Liu
authors.forename: Jianguo
authors.initials: J
authors.lastname: Li
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Li
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Kong
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yao
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Hu
authors.forename: Suyun
authors.initials: S
authors.lastname: Qian
authors.forename: Rong
authors.initials: R
authors.lastname: Geng
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Guy
authors.initials: G
authors.lastname: Vernet
authors.forename: Gláucia
authors.initials: G
authors.lastname: Paranhos-Baccalà
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
authors.forename: Kunling
authors.initials: K
authors.lastname: Shen
authors.forename: Jianwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009297:Nasal Mucosa / Q000821:virology; D009305:Nasopharynx / Q000821:virology; D010614:Pharynx / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011120:Polyomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral
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