Interleukin-20 targets renal mesangial cells and is associated with lupus nephritis.
Lupus nephritis is one manifestation of systemic lupus erythematosus (SLE). Interleukin (IL)-10 is involved in the pathogenesis of SLE. To determine whether IL-20, a member of the IL-10 family, is associated with lupus nephritis, we analyzed the expression of IL-20 and its receptors in mesangial cells derived from SLE-prone, NZB/W, and DBA/W mice. IL-20 and its receptors were upregulated in mesangial cells from NZB/W mice. Incubating IL-20 with mesangial cells upregulated the transcripts of CCL2 (MCP-1), CCL5 (RANTES), CXCL10 (IP-10), IL-6, iNOS, and ROS, all of which are involved in the pathogenesis of lupus nephritis. IL-20 specifically activated the downstream signal ERK 1/2. We also detected human IL-20 protein in both mesangial cells and inflammatory cells in kidney biopsies of patients with lupus nephritis. Our results reveal the novel effects of IL-20 on mesangial cells and its association with lupus nephritis.
Li
Hsing-Hui
HH
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Cheng
He-Hsiung
HH
Sun
Kuang-Hui
KH
Wei
Chi-Chen
CC
Li
Chien-Feng
CF
Chen
Wen-Chung
WC
Wu
Wen-Mein
WM
Chang
Ming-Shi
MS
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis; D002648:Child; D016207:Cytokines / Q000096:biosynthesis; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000502:physiology*; D008181:Lupus Nephritis / Q000209:etiology*; D008297:Male; D050527:Mesangial Cells / Q000378:metabolism*; D051379:Mice; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D017382:Reactive Oxygen Species; D013154:Spleen / Q000276:immunology
D018925:Chemokines; D016207:Cytokines; D007378:Interleukins; D017382:Reactive Oxygen Species; D052247:Nitric Oxide Synthase Type II; D048049:Extracellular Signal-Regulated MAP Kinases; C422042:interleukin 20