Gelatin-coated magnetic iron oxide nanoparticles as carrier system: drug loading and in vitro drug release study.
Magnetic iron oxide nanoparticles (IOPs) were coated with gelatin A and B and drug-loading efficiency was investigated using doxorubicin (DXR) as a model drug to evaluate their potential as a carrier system for magnetic drug targeting. Drug loading to coated IOPs was done using adsorption as well as desolvation/cross-linking techniques to understand their role. Drug loading by adsorption technique was done by incubating mixture of coated IOPs and drug in various conditions of pH, DXR-to-coated IOPs ratio, gelatin types and IOPs amounts. Drug loading by desolvation/cross-linking technique was done by adding acetone and glutaraldehyde (GTA) to the mixture of coated IOPs and DXR. The results indicated involvement of electrostatic interaction during loading of DXR-to-coated IOPs. Compared to adsorption technique, desolvation/cross-linking technique improved the efficiency of drug loading regardless of type of gelatin used for the coating. The DXR-loaded particles showed pH responsive drug release leading to accelerate release of drug at pH 4 compared to pH 7.4.
Gaihre
Babita
B
Department of Bionanosystem Engineering, Chonbuk National University, Chonju 561-756, Republic of Korea.
Khil
Myung Seob
MS
Lee
Douk Rae
DR
Kim
Hak Yong
HY
D000096:Acetone / Q000737:chemistry; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage; D002417:Cattle; D002626:Chemistry, Pharmaceutical; D003432:Cross-Linking Reagents / Q000737:chemistry; D004317:Doxorubicin / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D005290:Ferric Compounds / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D053758:Nanoparticles; D055672:Static Electricity; D013552:Swine; D013678:Technology, Pharmaceutical
D000903:Antibiotics, Antineoplastic; D003432:Cross-Linking Reagents; D005290:Ferric Compounds; D000096:Acetone; C000499:ferric oxide; D004317:Doxorubicin; D005780:Gelatin; D005976:Glutaral