Thrombin-activatable fibrinolysis inhibitor polymorphisms and recurrent pregnancy loss.
OBJECTIVE
To investigate the possible association between selected thrombin-activatable fibrinolysis inhibitor (TAFI) single nucleotide polymorphisms (SNPs) and recurrent pregnancy loss (RPL).


METHODS
Case-control study.


METHODS
University hospital.


METHODS
One hundred fifty-eight women (86 cases and 72 controls).


METHODS
Determination of TAFI SNPs -438A/G, +505A/G, +1040T/C, +1542C/G, and +1583A/T by polymerase chain reaction (PCR) reactions and sequencing analysis performed on peripheral blood samples.


METHODS
Analysis of the genotype and allele frequencies of TAFI SNPs -438A/G, +505A/G, +1040T/C, +1542C/G, and +1583A/T in women with and without RPL.


RESULTS
Genotype and allele frequencies of TAFI +505 and +1583 SNPs were significantly different in women with RPL compared with control women. The frequencies of the +505A/A and +505G/G genotypes were 1.2% and 61.6% in women with RPL and 13.9% and 43.1% in control women, respectively. The frequencies of the +1583A/A and +1583T/T genotypes were 1.2% and 61.6% in women with RPL and 13.9% and 45.8% in control women, respectively. The genotype and allele frequencies at TAFI position -438, +1040, and +1542 were not significantly different between RPL and control women.


CONCLUSIONS
The SNPs leading to increased TAFI levels are associated with a reduced risk of RPL. It is possible that TAFI is involved in RPL.
Masini
Silvia
S
Department of Internal Medicine, University Tor Vergata, Rome, Italy.
Ticconi
Carlo
C
Gravina
Paolo
P
Tomassini
Monica
M
Pietropolli
Adalgisa
A
Forte
Vittorio
V
Federici
Giorgio
G
Piccione
Emilio
E
Bernardini
Sergio
S
D000026:Abortion, Habitual / Q000453:epidemiology*; D000328:Adult; D025901:Carboxypeptidase B2 / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D006801:Humans; D015994:Incidence; D007558:Italy / Q000453:epidemiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012008:Recurrence; D018570:Risk Assessment; D012307:Risk Factors; D055815:Young Adult
D025901:Carboxypeptidase B2