The assay of gosling hepatitis virus and antibody by spermagglutination and spermagglutination-inhibition. I. Spermagglutination.
Agglutination of bovine spermatozoa by gosling hepatitis virus grown in embryonated eggs of 3 different hosts and from several different passage levels was demonstrated. A good agreement was found between the egg-infective and spermagglutinating titres of the virus. The reaction closely resembles that previously described for Newcastle disease virus, influenza virus and Mycoplasma. Gosling hepatitis virus differs however from the other spermagglutinating agents by its inability to agglutinate erythrocytes. Optimal physico-chemical requirements for the reaction were determined. The spermagglutination technique provides a rapid and simple method for assaying the virus. The method is also convenient and inexpensive owing to the availability of spermatozoa in pelleted form which can be stored frozen for long periods.
Peleg
B A
BA
Tel Aviv University, Kimron Veterinary Institute, Israel.
Malkinson
M
M
Ron
N
N
Kalmar
E
E