title: Blood components produced from whole blood using the Atreus processing system.
abstract: BACKGROUND
  The Atreus 2C+ system (Gambro BCT) automates whole blood (WB) processing into a single device. This study compared the quality of red blood cells (RBCs), fresh-frozen plasma (FFP), and buffy coats (BCs) made from WB held with or without active cooling.
  
  
  METHODS
  WB was collected into Atreus disposables and stored with (n = 20) or without (n = 20) active cooling for 14 to 18 hours at 22 +/- 2 degrees C before processing with the Atreus. Two RBC leukodepletion filters were assessed, and markers of RBC quality were tested to Day 42. BCs were held for 3 hours before testing, plasma was tested, and samples were frozen for coagulation analysis.
  
  
  RESULTS
  RBCs met UK specifications for volume, hemoglobin content (48 +/- 5 g), and hematocrit (Hct). Hemolysis, adenosine triphosphate, 2,3-diphosphoglycerate, potassium, glucose, and lactate throughout storage were all within expected ranges. No differences were seen in RBC produced from WB held with or without active cooling. FFP units met UK specification for volume, total protein, cellular contamination, and coagulation factors. No differences were seen in FFP produced from WB held with or without active cooling. The Hct of BCs produced from WB held without active cooling was lower than in BCs from WB held with active cooling; no differences in activation were seen.
  
  
  CONCLUSIONS
  From these in vitro data, blood components produced using the Atreus appear suitable for clinical use, with no clinically significant difference in the quality of components from WB held at ambient temperature overnight with or without active cooling.
authors.affiliation: Components Development Laboratory, NHS Blood & Transplant, Brentwood, UK. stephen.thomas@nbs.nhs.uk.
authors.forename: Stephen
authors.initials: S
authors.lastname: Thomas
authors.forename: Martin
authors.initials: M
authors.lastname: Beard
authors.forename: Margaret
authors.initials: M
authors.lastname: Garwood
authors.forename: Martine
authors.initials: M
authors.lastname: Callaert
authors.forename: Geert van
authors.initials: Gv
authors.lastname: Waeg
authors.forename: Rebecca
authors.initials: R
authors.lastname: Cardigan
mesh_terms: D001781:Blood Component Removal / Q000379:methods*; D001793:Blood Preservation / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis; D004912:Erythrocytes / Q000382:microbiology; D005740:Gases; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D013997:Time Factors
chemical_list: D001798:Blood Proteins; D005740:Gases