title: Role of ethylene diamine tetra-acetic acid (EDTA) in catheter lock solutions: EDTA enhances the antifungal activity of amphotericin B lipid complex against Candida embedded in biofilm.
abstract: Ethylene diamine tetra-acetic acid (EDTA) is an anticoagulant with antibiofilm-enhancing activity. We therefore used an in vitro biofilm model to determine the activity of amphotericin B lipid complex (ABLC) with or without EDTA against Candida embedded in biofilm on silicone disk surfaces. Clinical blood isolates from cancer patients infected with Candida albicans or Candida parapsilosis were used. Silicone disks were colonised with C. albicans or C. parapsilosis and were sequentially incubated in plasma and then in Mueller-Hinton broth containing 10(5) colony-forming units of each organism. All tests were performed in triplicate. The disks were subsequently placed and incubated for 6h and 8h in solutions containing ABLC alone, EDTA alone, ABLC+EDTA or broth (control). Disks were then removed, sonicated and colony counts were determined. ABLC+EDTA (30 mg/mL) was significantly more effective than ABLC, EDTA and control against C. parapsilosis at 6h (P < or = 0.01) and against C. albicans at 8h (P < or = 0.04). In patients with catheter-related candidaemia when catheter removal is not feasible, the combination of ABLC+EDTA may be considered for antifungal catheter lock solution as part of a catheter salvage therapy.
authors.affiliation: Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA. iraad@mdanderson.org
authors.forename: Issam I
authors.initials: II
authors.lastname: Raad
authors.forename: Ray Y
authors.initials: RY
authors.lastname: Hachem
authors.forename: Hend A
authors.initials: HA
authors.lastname: Hanna
authors.forename: Xiang
authors.initials: X
authors.lastname: Fang
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Tanya
authors.initials: T
authors.lastname: Dvorak
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Sherertz
authors.forename: Dimitrios P
authors.initials: DP
authors.lastname: Kontoyiannis
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002175:Candida / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D055499:Catheter-Related Infections / Q000517:prevention & control*; D002614:Chelating Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004357:Drug Synergism; D004492:Edetic Acid / Q000494:pharmacology*; D019999:Pharmaceutical Solutions
chemical_list: D000935:Antifungal Agents; D002614:Chelating Agents; D019999:Pharmaceutical Solutions; C068538:liposomal amphotericin B; D000666:Amphotericin B; D004492:Edetic Acid