title: Metabolic environment in substantia nigra reticulata is critical for the expression and control of hypoglycemia-induced seizures.
abstract: Seizures represent a common and serious complication of hypoglycemia. Here we studied mechanisms of control of hypoglycemic seizures induced by insulin injection in fasted and nonfasted rats. We demonstrate that fasting predisposes rats to more rapid and consistent development of hypoglycemic seizures. However, the fasting-induced decrease in baseline blood glucose concentration cannot account for the earlier onset of seizures in fasted versus nonfasted rats. Data obtained with c-Fos immunohistochemistry and [14C]2-deoxyglucose uptake implicate a prominent involvement of the substantia nigra reticulata (SNR) among other structures in the hypoglycemic seizure control. This is supported by data showing that fasting decreases the SNR expression of K(ATP) channels, which link metabolism with activity, and is further confirmed with microinfusions of K(ATP) channel agonist and antagonist. Data obtained with whole-cell and perforated patch recordings from SNR neurons in slices in vitro demonstrate that both presynaptic and postsynaptic K(ATP) channels participate in the failure of the SNR to control hypoglycemic seizures. The results suggest that fasting and insulin-induced hypoglycemia can lead to impairment in the function of the SNR, leading thus to hypoglycemic seizures.
authors.affiliation: The Saul R. Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. velisek@aecom.yu.edu
authors.forename: Libor
authors.initials: L
authors.lastname: Velísek
authors.forename: Jana
authors.initials: J
authors.lastname: Velísková
authors.forename: Ondrej
authors.initials: O
authors.lastname: Chudomel
authors.forename: Ka-Lai
authors.initials: KL
authors.lastname: Poon
authors.forename: Kimberly
authors.initials: K
authors.lastname: Robeson
authors.forename: Barbara
authors.initials: B
authors.lastname: Marshall
authors.forename: Archana
authors.initials: A
authors.lastname: Sharma
authors.forename: Solomon L
authors.initials: SL
authors.lastname: Moshé
mesh_terms: D000818:Animals; D003847:Deoxyglucose; D004734:Energy Metabolism / Q000502:physiology*; D005508:Food Deprivation / Q000502:physiology; D007003:Hypoglycemia / Q000150:complications* / Q000378:metabolism / Q000503:physiopathology; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology; D054086:KATP Channels / Q000378:metabolism; D008297:Male; D009924:Organ Culture Techniques; D018408:Patch-Clamp Techniques; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000209:etiology* / Q000378:metabolism* / Q000503:physiopathology; D013378:Substantia Nigra / Q000378:metabolism* / Q000503:physiopathology; D000099161:Kcnj11 Channel
chemical_list: D003847:Deoxyglucose; D007328:Insulin; D054086:KATP Channels; D024661:Potassium Channels, Inwardly Rectifying; D016760:Proto-Oncogene Proteins c-fos; D000099161:Kcnj11 Channel
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