title: Oligonucleotides direct synthesis on porous silicon chip.
abstract: A solid phase oligonucleotide (ON) synthesis on porous silicon (PSi) chip is presented. The prepared Si-OH surface were analyzed by FT-IR and the OH functions were quantified by reaction with 3'-phosphoramidite nucleotide building block. Short ONs were synthesized on the chip surface and the coupling yields evaluated.
authors.affiliation: Istituto per la Microelettronica e Microsistemi - Unità di Napoli, Consiglio Nazionale delle Ricerche, via P. Castellino 111, I-80131 Napoli, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: De Stefano
authors.forename: Edoardo
authors.initials: E
authors.lastname: De Tommasi
authors.forename: Ilaria
authors.initials: I
authors.lastname: Rea
authors.forename: Lucia
authors.initials: L
authors.lastname: Rotiroti
authors.forename: Luca
authors.initials: L
authors.lastname: Giangrande
authors.forename: Giorgia
authors.initials: G
authors.lastname: Oliviero
authors.forename: Nicola
authors.initials: N
authors.lastname: Borbone
authors.forename: Aldo
authors.initials: A
authors.lastname: Galeone
authors.forename: Gennaro
authors.initials: G
authors.lastname: Piccialli
mesh_terms: D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*
chemical_list: D015345:Oligonucleotide Probes; D012825:Silicon