[Study on Acinetobacter baumannii plasmid with 3 types of beta-lactamase genes in a burn ward].
OBJECTIVE
To study the transferrable character of Acinetobacter baumannii (AB) plasmids with 3 types of beta-lactamase gene.


METHODS
The plasmid of multi-drug resistant AB (donor) isolated from burn wound were transferred to E. coil ATCC25922 (receptor) through conjugation, and drug sensitivity was also observed. Drug-resistant gene and stability of filial generation and zygote were analyzed by PCR.


RESULTS
The drug-resistance of donor plasmids to Sulfamethoxazole, Ampicillin, Cefalotin, Cefpodoxime, Cefuroxime, Imipenem/Cilastatin and Ampicillin/Sulbactam, and three types of beta-lactamase gene were transferred to the receptor, and were also stably transmitted for passages. The minimum inhibitor concentration of receptor to Sulfamethoxazole was > 2 mg/L after conjugation with donor, and inhibitory character could be transferred to next generation.


CONCLUSIONS
bla(TEM-1), bla(PER-1) and bla(OXA-23) genes carried in the plasmid of AB can be transferred through conjugation and stably transmitted for passages, and it is one of the molecular mechanisms for AB with multi-drug resistance after burn infections.
Li
Rong
R
Department of Microbiology, School of Basic Medical Sciences of Nanchang University, Nanchang 330006, PR China.
Li
Wen-Lin
WL
Shi
Xiao-Yu
XY
Zeng
Yuan-Lin
YL
Xv
Xiao-Wen
XW
Zhao
Lin
L
D000151:Acinetobacter Infections; D040981:Acinetobacter baumannii / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D002056:Burns / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases