Polyamine biosynthesis regulated by StARD expression plays an important role in potato wound periderm formation.
An acireductone dioxygenase (ARD) gene of potatoes was isolated from the expressed sequence tags (ESTs) of potato post-suberization cDNA libraries. The highest expression levels of the StARD gene and the protein appeared 36 h after suberization. An approximate 9-fold increase in ARD activity was detected at 36 h after wounding. Real-time reverse transcription-PCR (RT-PCR) analysis and immunolocalization studies revealed that StARD transcripts increase at the wound surface of potato tubers. The polyamine (PA) contents increased significantly after wounding at the wound surface. The increased PA content and ARD activity may play an important role in wound periderm formation.
Kim
Jae Hyun
JH
Plant Genome Research Center, KRIBB, Daejeon 305-806, Korea.
Kim
Hyun Soon
HS
Lee
Yong Hwa
YH
Kim
Yoon Sik
YS
Oh
Hyun Woo
HW
Joung
Hyouk
H
Chae
Suhn Kee
SK
Suh
Kyong Hoon
KH
Jeon
Jae Heung
JH
D049308:Dioxygenases / Q000378:metabolism*; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D008055:Lipids / Q000096:biosynthesis; D008715:Methionine / Q000378:metabolism; D035281:Plant Tubers / Q000235:genetics / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics
D008055:Lipids; D011073:Polyamines; D018749:RNA, Plant; C065875:suberin; D008715:Methionine; D049308:Dioxygenases; C083734:aci-reductone oxidase (CO-forming)