title: Alcohol and nicotine consumption exacerbates choroidal neovascularization by modulating the regulation of complement system.
abstract: The objective of the present study was to investigate the effect of alcohol and nicotine consumption on the pathogenesis of choroidal neovascularization (CNV) in rats after laser-photocoagulation. Confocal microscopic analysis demonstrated an increase in CNV complex size in rats fed with alcohol (2.3-fold), nicotine (1.9-fold), and the combination of alcohol and nicotine (2.7-fold) compared with the control groups. Immunohistochemical analysis revealed that alcohol and nicotine consumption increased MAC deposition and VEGF expression in laser spots. Expression of CD59 by RT-PCR and Western blot was drastically reduced in the animals that were fed with alcohol, nicotine and alcohol and nicotine compared to those fed with water alone and this was associated with exacerbation of CNV.
authors.affiliation: Department of Ophthalmology, Pat and Willard Walker Eye Research Center, Jones Eye Institute, 4301 West Markham, Mail Slot 523, Little Rock, AR 72205, United States.
authors.forename: Sankaranarayanan
authors.initials: S
authors.lastname: Kaliappan
authors.forename: Purushottam
authors.initials: P
authors.lastname: Jha
authors.forename: Valeriy V
authors.initials: VV
authors.lastname: Lyzogubov
authors.forename: Ruslana G
authors.initials: RG
authors.lastname: Tytarenko
authors.forename: Nalini S
authors.initials: NS
authors.lastname: Bora
authors.forename: Puran S
authors.initials: PS
authors.lastname: Bora
mesh_terms: D000428:Alcohol Drinking / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D020256:Choroidal Neovascularization / Q000378:metabolism / Q000473:pathology*; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D008297:Male; D009538:Nicotine / Q000633:toxicity*; D051381:Rats; D011914:Rats, Inbred BN
chemical_list: D015938:Complement Membrane Attack Complex; D000431:Ethanol; D009538:Nicotine
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