Toward a prostate specific antigen-based prostate cancer diagnostic assay: preparation of keyhole limpet hemocyanin-conjugated normal and transformed prostate specific antigen fragments.
Prostate specific antigen (PSA) molecules secreted by cancerous and normal prostate cells differ in their N-linked glycan composition, while the peptide backbone appears to be conserved. Antibodies selectively recognizing such differentially glycosylated PSA structures could form a basis for a new diagnostic assay for prostate cancer. Twenty-amino acid PSA fragments carrying di-, tri-, and tetrabranched complex-type glycans were prepared by total synthesis and conjugated to maleimide-modified keyhole limpet hemocyanin (KLH) carrier protein through backbone Cys residues. These glycopeptide/KLH conjugates were then used for antibody generation.
Dudkin
Vadim Y
VY
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA.
Miller
Justin S
JS
Dudkina
Anna S
AS
Antczak
Christophe
C
Scheinberg
David A
DA
Danishefsky
Samuel J
SJ
D000906:Antibodies / Q000276:immunology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006433:Hemocyanins / Q000737:chemistry*; D006801:Humans; D008297:Male; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D010446:Peptide Fragments; D006433:Hemocyanins; D017430:Prostate-Specific Antigen
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