Cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Lactobacillus plantarum LA 318 recognizes human A and B blood group antigens.
Lactobacillus plantarum LA 318 is a potential probiotic strain isolated from normal human intestinal tissue that shows high adhesion to human colonic mucin mediated by the bacterial cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report the adhesion mechanism of the lactobacilli is in part due to GAPDH binding to human ABO-type blood group antigens expressed on human colonic mucin (HCM). After periodate oxidation of HCM, adhesion of L. plantarum LA 318 bacterial cells significantly decreased compared to normal HCM. A BIACORE binding assay of GAPDH to blood group antigens was then performed. High binding was observed to A and B group antigens, while binding to H group antigen was lower (P<0.01). No interaction was observed between GAPDH and various monosaccharides. Furthermore, GAPDH binding to the B-trisaccharide biotinyl polymer (BP)-probe [Galalpha1-3 (Fucalpha1-2) Gal-] was significantly higher as compared to B-disaccharide, Lewis D-trisaccharide, 3-fucosyl-N-acetylglucosamine and alpha-N-acetylneuraminic acid BP-probes. The data suggests the trisaccharide structure is important in binding to the blood group antigens. The binding of GAPDH to HCM significantly decreased after incubation with NAD+. This suggests that the NAD binding domain on GAPDH may be related to binding to HCM.
Kinoshita
Hideki
H
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1,Aoba-ku, Sendai, Miyagi 981-8555, Japan. kino0328@bios.tohoku.ac.jp
Wakahara
Nozomi
N
Watanabe
Masamichi
M
Kawasaki
Tomomi
T
Matsuo
Hiroki
H
Kawai
Yasushi
Y
Kitazawa
Haruki
H
Ohnuma
Sinobu
S
Miura
Koh
K
Horii
Akira
A
Saito
Tadao
T
D000017:ABO Blood-Group System / Q000737:chemistry / Q000378:metabolism*; D001422:Bacterial Adhesion; D003106:Colon / Q000378:metabolism / Q000382:microbiology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology; D048191:Lactiplantibacillus plantarum / Q000201:enzymology* / Q000302:isolation & purification / Q000502:physiology*; D009077:Mucins / Q000378:metabolism*; D019936:Probiotics / Q000302:isolation & purification
D000017:ABO Blood-Group System; D009077:Mucins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases