title: [MicroRNA differential expression profile during differentiation of embryonic stem cells towards hepatocytes induced by sodium butyrate].
abstract: OBJECTIVE
  To explore the expression profile of microRNAs during the course of embryonic stem cells differentiation towards hepatocytes induced by sodium butyrate.
  
  
  METHODS
  Total RNA was extracted from embryonic stem cells on day 0, 6, and 9 during cell differentiation, and microRNA was isolated from the total RNA. Microarray analysis of microRNA expression was performed to detect the different expression levels of microRNA among the indicated time points (day 0, 6, and 9).
  
  
  RESULTS
  Compared with the microRNA expression level on day 0 of cell differentiation, 17 different microRNAs exhibited higher expressions both on day 6 and day 9. Twenty-two and 27 microRNA demonstrated lower expressions on day 6 and day 9, respectively. Further analysis revealed that 15 microRNA among the above microRNAs with significant differential expression may keep close interation with histone deacetylase.
  
  
  CONCLUSIONS
  During the course of embryonic stem cells differentiation towards hepatocytes induced by sodium butyrate, histone deacetylase and its relevant microRNAs may play important roles in cell differentiation.
authors.affiliation: Department of Hepatobiliary Surgery, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Ya-jin
authors.initials: YJ
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Min
authors.forename: Chang-zhen
authors.initials: CZ
authors.lastname: Shang
authors.forename: Meng
authors.initials: M
authors.lastname: Ren
authors.forename: Xiao-xiong
authors.initials: XX
authors.lastname: Peng
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Ji-sheng
authors.initials: JS
authors.lastname: Chen
mesh_terms: D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism
chemical_list: D002087:Butyrates; D035683:MicroRNAs