title: Synthesis and silencing properties of siRNAs possessing lipophilic groups at their 3'-termini.
abstract: Short interfering RNAs (siRNAs) conjugated with lipophilic groups at their 3'-termini were synthesized. The silencing activities of these siRNAs were examined by a dual-luciferase assay. It was found that the '-end of the passenger strand (sense strand) was better than the guide strand (antisense strand) for connecting bulky lipophilic molecules, particularly a rigid molecule such as cholesterol.
authors.affiliation: Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501-1193, Japan. uenoy@gifu-u.ac.jp
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ueno
authors.forename: Koshi
authors.initials: K
authors.lastname: Kawada
authors.forename: Aya
authors.initials: A
authors.lastname: Shibata
authors.forename: Kayo
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Wataya
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008156:Luciferases / Q000235:genetics; D019301:Oleic Acid / Q000737:chemistry; D019308:Palmitic Acid / Q000737:chemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D008055:Lipids; D034741:RNA, Small Interfering; D019301:Oleic Acid; D019308:Palmitic Acid; D002784:Cholesterol; D008156:Luciferases