A rapid and hazardous reagent free protocol for genomic DNA extraction suitable for genetic studies in plants.
Protocols for genomic DNA extraction from plants are generally lengthy, since they require that tissues be ground in liquid nitrogen, followed by a precipitation step, washing and drying of the DNA pellet, etc. This represents a major challenge especially when several hundred samples must be screened/analyzed within a working day. There is therefore a need for a rapid and simple procedure, which will produce DNA quality suitable for various analyses. Here, we describe a time and cost efficient protocol for genomic DNA isolation from plants suitable for all routine genetic screening/analyses. The protocol is free from hazardous reagents and therefore safe to be executed by non-specialists. With this protocol more than 100 genomic DNA samples could manually be extracted within a working day, making it a promising alternative in genetic studies of large-scale genomic screening projects.
Kotchoni
Simeon O
SO
Agronomy Department, Purdue University, Lilly Hall, 915 West State Street, West Lafayette, IN 47907, USA. skotchon@purdue.edu
Gachomo
Emma W
EW
D018744:DNA, Plant / Q000302:isolation & purification*; D005821:Genetic Techniques; D018745:Genome, Plant; D010944:Plants / Q000235:genetics*; D012107:Research Design
D018744:DNA, Plant
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