title: Trypsin inhibitors in passion fruit (Passiflora f. edulis flavicarpa) leaves: accumulation in response to methyl jasmonate, mechanical wounding, and herbivory.
abstract: This work investigates the effect of methyl jasmonte (MeJa), mechanical wounding, and herbivory caused by larval feeding of a specialist insect ( Agraulis vanillae vanillae) upon trypsin inhibitory activity in passion fruit leaves. Despite the fact that all treatments caused accumulation of trypsin inhibitors (TIs), higher levels were observed in MeJa treated leaves when plants were assayed 24 and 48 h after stimulus. Concerning both mechanically injured plants and attacked ones, a systemic induction was observed. Partially purified inhibitors from MeJa exposed plants were further characterized by X-ray film contact print technique and N-terminal sequence. Such analysis indicated that the TIs identified belong to the Kunitz family. Moreover, the partially purified inhibitors strongly inhibited trypsin-like digestive enzymes from sugar cane stalk borer ( Diatraea saccharalis) in vitro. Our results further support the protective function of wound-inducible trypsin inhibitors and their potential as tools to improve important crop species against insect predation through genetic engineering.
authors.affiliation: Laboratorio de Biotecnologia, Universidade Estadual do Norte Fluminense, 28013-600 Campos dos Goytacazes, RJ, Brazil.
authors.forename: Sylvio
authors.initials: S
authors.lastname: Botelho-Júnior
authors.forename: César L
authors.initials: CL
authors.lastname: Siqueira-Júnior
authors.forename: Bruno C
authors.initials: BC
authors.lastname: Jardim
authors.forename: Olga L T
authors.initials: OL
authors.lastname: Machado
authors.forename: Ana G C
authors.initials: AG
authors.lastname: Neves-Ferreira
authors.forename: Jonas
authors.initials: J
authors.lastname: Perales
authors.forename: Tânia
authors.initials: T
authors.lastname: Jacinto
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D002080:Butterflies / Q000502:physiology*; D003517:Cyclopentanes / Q000494:pharmacology*; D005247:Feeding Behavior; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects; D054883:Oxylipins / Q000494:pharmacology*; D029598:Passiflora / Q000187:drug effects / Q000502:physiology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D016415:Sequence Alignment; D013314:Stress, Mechanical; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010936:Plant Extracts; D010940:Plant Proteins; D014361:Trypsin Inhibitors; C072239:methyl jasmonate