title: Mypt1-mediated spatial positioning of Bmp2-producing cells is essential for liver organogenesis.
abstract: Mesodermal tissues produce various inductive signals essential for morphogenesis of endodermal organs. However, little is known about how the spatial relationship between the mesodermal signal-producing cells and their target endodermal organs is established during morphogenesis. Here, we report that a mutation in the zebrafish myosin phosphatase targeting subunit 1 (mypt1) gene causes abnormal bundling of actin filaments and disorganization of lateral plate mesoderm (LPM) and endoderm cells. As a result, the coordination between mesoderm and endoderm cell movements is disrupted. Consequently, the two stripes of Bmp2a-expressing cells in the LPM fail to align in a V-shaped pocket sandwiching the liver primordium. Mispositioning Bmp2a-producing cells with respect to the liver primordium leads to a reduction in hepatoblast proliferation and final abortion of hepatoblasts by apoptosis, causing the liverless phenotype. Our results demonstrate that Mypt1 mediates coordination between mesoderm and endoderm cell movements in order to carefully position the liver primordium such that it receives a Bmp signal that is essential for liver formation in zebrafish.
authors.affiliation: Laboratory of Functional Genomics, Institute of Molecular and Cell Biology, Proteos, Singapore.
authors.forename: Honghui
authors.initials: H
authors.lastname: Huang
authors.forename: Hua
authors.initials: H
authors.lastname: Ruan
authors.forename: Meng Yuan
authors.initials: MY
authors.lastname: Aw
authors.forename: Alamgir
authors.initials: A
authors.lastname: Hussain
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.forename: Chuan
authors.initials: C
authors.lastname: Gao
authors.forename: Feng
authors.initials: F
authors.lastname: Qian
authors.forename: Thomas
authors.initials: T
authors.lastname: Leung
authors.forename: Haiwei
authors.initials: H
authors.lastname: Song
authors.forename: David
authors.initials: D
authors.lastname: Kimelman
authors.forename: Zilong
authors.initials: Z
authors.lastname: Wen
authors.forename: Jinrong
authors.initials: J
authors.lastname: Peng
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D030801:Animals, Genetically Modified; D017209:Apoptosis; D001483:Base Sequence; D019521:Body Patterning / Q000235:genetics / Q000502:physiology; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis* / Q000235:genetics; D002465:Cell Movement; D049109:Cell Proliferation; D004247:DNA / Q000235:genetics; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D008648:Mesoderm / Q000196:embryology / Q000378:metabolism; D009154:Mutation; D043263:Myosin-Light-Chain Phosphatase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054645:Protein Phosphatase 1 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D055396:Bone Morphogenetic Protein 2; D029961:Zebrafish Proteins; D004247:DNA; D054645:Protein Phosphatase 1; D043263:Myosin-Light-Chain Phosphatase
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