title: Fluorogenic probe triggered by reduction for nucleic acids sensing.
abstract: A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10-20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Furukawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Kazuma
authors.initials: K
authors.lastname: Oki
authors.forename: Miwako
authors.initials: M
authors.lastname: Uda
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsuneda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D009841:Oligonucleotides / Q000032:analysis*; D010084:Oxidation-Reduction; D012338:RNA, Ribosomal, 23S / Q000032:analysis; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012338:RNA, Ribosomal, 23S; D012235:Rhodamines