title: A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.
abstract: Centrosomes comprise a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Here, we have performed a microscopy-based genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins required for centriole duplication and mitotic PCM recruitment. We analysed 92% of the Drosophila genome (13,059 genes) and identified 32 genes involved in centrosome function. An extensive series of secondary screens classified these genes into four categories: (1) nine are required for centriole duplication, (2) 11 are required for centrosome maturation, (3) nine are required for both functions, and (4) three genes regulate centrosome separation. These 32 hits include several new centrosomal components, some of which have human homologs. In addition, we find that the individual depletion of only two proteins, Polo and Centrosomin (Cnn) can completely block centrosome maturation. Cnn is phosphorylated during mitosis in a Polo-dependent manner, suggesting that the Polo-dependent phosphorylation of Cnn initiates centrosome maturation in flies.
authors.affiliation: The Gurdon Institute, Cambridge, United Kingdom.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Dobbelaere
authors.forename: Filipe
authors.initials: F
authors.lastname: Josué
authors.forename: Saskia
authors.initials: S
authors.lastname: Suijkerbuijk
authors.forename: Buzz
authors.initials: B
authors.lastname: Baum
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tapon
authors.forename: Jordan
authors.initials: J
authors.lastname: Raff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002502:Centrioles / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism* / Q000648:ultrastructure; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D016678:Genome; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; C098130:cnn protein, Drosophila; C072004:polo protein, Drosophila; D017346:Protein Serine-Threonine Kinases
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