Single-strand binding protein enhances invasion of a PNA strand to double-stranded DNA.
We show that, in the presence of single-strand binding protein (SSB), one strand of peptide nucleic acid (PNA) efficiently invades double-stranded DNA (dsDNA) in sequence-specific manner, as shown in Figure 1. In the absence of SSB, this kind of strand invasion never occurs. This significant enhancement of invasion can be applied to in vivo applications.
Ishizuka
Takumi
T
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, Japan.
Komiyama
Makoto
M
D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D004268:DNA-Binding Proteins; D020135:Peptide Nucleic Acids; D004247:DNA