title: Dynamic localization of MreB in Vibrio parahaemolyticus and in the ectopic host bacterium Escherichia coli.
abstract: MreB, a homolog of eukaryotic actin, participates in morphogenesis, cell division, cell polarity, and chromosome segregation in prokaryotes. In this study, a yellow fluorescent protein conjugate (YFP-MreB(Vp)) was generated to investigate the behavior of MreB in merodiploid strain SC9 of the enteropathogen Vibrio parahaemolyticus. Under normal growth conditions, YFP-MreB(Vp) formed helical filaments with a pitch of 0.64 +/- 0.09 microm in about 85% of exponential-phase cells, and different clusters, relaxed coils, and ring configurations were observed in a small proportion of the cells. Overexpression of YFP-MreB(Vp) substantially altered the structure of the MreB cytoskeleton and resulted in swollen and pleomorphic cells. Disturbing the activities of penicillin-binding proteins or adding magnesium suppressed the morphological distortions. These results indicate that mislocalization of cell wall-synthesizing machinery was responsible for morphological abnormality. By expressing YFP-MreB(Vp) in the ectopic host bacterium Escherichia coli, shrinkage, fragmentation, and annealing of MreB(Vp) filaments were directly observed. This work revealed the dynamic pattern of the localization of YFP-MreB(Vp) in V. parahaemolyticus and its relationship to cell morphogenesis, and the YFP-MreB(Vp)-E. coli system may be used to investigate the dynamic spatial structures of the MreB cytoskeleton in vivo.
authors.affiliation: Department of Microbiology, Soochow University, Taipei, Taiwan, Republic of China.
authors.forename: Shen-Wen
authors.initials: SW
authors.lastname: Chiu
authors.forename: Shau-Yan
authors.initials: SY
authors.lastname: Chen
authors.forename: Hin-chung
authors.initials: HC
authors.lastname: Wong
mesh_terms: D000199:Actins / Q000378:metabolism*; D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D023061:Gene Order; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D005810:Multigene Family; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D026801:Synteny; D014736:Vibrio parahaemolyticus / Q000737:chemistry*
chemical_list: D000199:Actins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C054562:yellow fluorescent protein, Bacteria; C078473:MreB protein, E coli
references.citation: J Bacteriol. 1994 Feb;176(3):917-22
references.pmid: 8300545
references.citation: Nucleic Acids Res. 2001 Jan 1;29(1):123-5
references.pmid: 11125067
references.citation: J Bacteriol. 2006 Feb;188(3):968-76
references.pmid: 16428401
references.citation: J Biol Chem. 2005 Jan 28;280(4):2628-35
references.pmid: 15548516
references.citation: Biosci Biotechnol Biochem. 2006 Nov;70(11):2712-9
references.pmid: 17090951
references.citation: J Bacteriol. 1999 Feb;181(4):1110-7
references.pmid: 9973335
references.citation: Curr Biol. 2007 Feb 6;17(3):266-72
references.pmid: 17276920
references.citation: Cell. 2001 Mar 23;104(6):913-22
references.pmid: 11290328
references.citation: Lancet. 2003 Mar 1;361(9359):743-9
references.pmid: 12620739
references.citation: Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7865-70
references.pmid: 12766229
references.citation: Mol Microbiol. 2004 Mar;51(5):1321-32
references.pmid: 14982627
references.citation: Mol Microbiol. 2007 Jul;65(1):51-63
references.pmid: 17581120
references.citation: EMBO J. 2002 Jul 1;21(13):3347-57
references.pmid: 12093736
references.citation: Mol Microbiol. 2005 Mar;55(6):1646-57
references.pmid: 15752190
references.citation: J Bacteriol. 1989 Jun;171(6):3123-7
references.pmid: 2656641
references.citation: Appl Environ Microbiol. 2000 Sep;66(9):3981-6
references.pmid: 10966418
references.citation: Proc Natl Acad Sci U S A. 2006 Jul 18;103(29):10929-34
references.pmid: 16829583
references.citation: J Bacteriol. 1998 Apr;180(8):2050-6
references.pmid: 9555885
references.citation: Microbiol Mol Biol Rev. 2006 Sep;70(3):729-54
references.pmid: 16959967
references.citation: Mol Microbiol. 2004 Jul;53(2):469-83
references.pmid: 15228528
references.citation: J Bacteriol. 2005 Jan;187(1):54-64
references.pmid: 15601688
references.citation: Cell. 2003 Jun 13;113(6):767-76
references.pmid: 12809607
references.citation: Proc Natl Acad Sci U S A. 2007 Nov 6;104(45):17795-800
references.pmid: 17978175
references.citation: Microbiol Mol Biol Rev. 2006 Jun;70(2):317-43
references.pmid: 16760306
references.citation: EMBO J. 2002 Jun 17;21(12):3119-27
references.pmid: 12065424
references.citation: EMBO Rep. 2004 Aug;5(8):789-94
references.pmid: 15272301
references.citation: Plasmid. 2004 May;51(3):246-55
references.pmid: 15109831
references.citation: J Bacteriol. 2007 Oct;189(20):7450-63
references.pmid: 17704222
references.citation: J Bacteriol. 1998 Sep;180(17):4555-63
references.pmid: 9721295
references.citation: Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8643-8
references.pmid: 15159537
references.citation: Science. 2004 Nov 5;306(5698):1021-5
references.pmid: 15528442
references.citation: Mol Microbiol. 2005 Sep;57(5):1196-209
references.pmid: 16101995
references.citation: Mol Microbiol. 2006 May;60(4):838-52
references.pmid: 16677297
references.citation: EMBO J. 2003 Oct 1;22(19):5283-92
references.pmid: 14517265
references.citation: Dev Cell. 2006 Sep;11(3):399-409
references.pmid: 16950129
references.citation: Proc Natl Acad Sci U S A. 2005 Dec 6;102(49):17658-63
references.pmid: 16306264
references.citation: Dev Cell. 2003 Jan;4(1):19-28
references.pmid: 12530960
references.citation: Mol Microbiol. 1999 Feb;31(4):1149-59
references.pmid: 10096082
references.citation: J Bacteriol. 1993 May;175(10):3139-45
references.pmid: 8387996
references.citation: Antimicrob Agents Chemother. 1999 Nov;43(11):2702-9
references.pmid: 10543750