title: Large-scale gene trapping in C57BL/6N mouse embryonic stem cells.
abstract: We report the construction and analysis of a mouse gene trap mutant resource created in the C57BL/6N genetic background containing more than 350,000 sequence-tagged embryonic stem (ES) cell clones. We also demonstrate the ability of these ES cell clones to contribute to the germline and produce knockout mice. Each mutant clone is identified by a genomic sequence tag representing the exact insertion location, allowing accurate prediction of mutagenicity and enabling direct genotyping of mutant alleles. Mutations have been identified in more than 10,000 genes and show a bias toward the first intron. The trapped ES cell lines, which can be requested from the Texas A&M Institute for Genomic Medicine, are readily available to the scientific community.
authors.affiliation: Lexicon Pharmaceuticals Incorporated, The Woodlands, Texas 77381, USA. ghansen@lexpharma.com
authors.forename: Gwenn M
authors.initials: GM
authors.lastname: Hansen
authors.forename: Diane C
authors.initials: DC
authors.lastname: Markesich
authors.forename: Michael B
authors.initials: MB
authors.lastname: Burnett
authors.forename: Qichao
authors.initials: Q
authors.lastname: Zhu
authors.forename: Karen M
authors.initials: KM
authors.lastname: Dionne
authors.forename: Lizabeth J
authors.initials: LJ
authors.lastname: Richter
authors.forename: Richard H
authors.initials: RH
authors.lastname: Finnell
authors.forename: Arthur T
authors.initials: AT
authors.lastname: Sands
authors.forename: Brian P
authors.initials: BP
authors.lastname: Zambrowicz
authors.forename: Alejandro
authors.initials: A
authors.lastname: Abuin
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002460:Cell Line; D002678:Chimera; D002999:Clone Cells; D004622:Embryo, Mammalian / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D007438:Introns; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D035683:MicroRNAs; D016254:Mutagenesis, Insertional
chemical_list: D035683:MicroRNAs
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