title: Dynamics of 2-N-tert-butylaminoxyladenosine incorporated into oligodeoxynucleotides.
abstract: We synthesized oligodeoxynucleotide (ODN, 3) containing 2-N-tert-butylaminoxyladenosine (1) and studied EPR spectra of 1 and its duplexes with varied sequences. Duplex formation resulted in a broadening of spectrum and a significant decrease in peak-to-peak height and peak height ratio values. The h(+)/ho values in EPR spectra well correlated with stability of duplex which indicated ODN containing 1 has the potential to monitor DNA structure.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. aso@phar.kyushu-u.ac.jp
authors.forename: Mariko
authors.initials: M
authors.lastname: Aso
authors.forename: Manami
authors.initials: M
authors.lastname: Kurita
authors.forename: John Walter
authors.initials: JW
authors.lastname: Mirc
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kaneko
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Noboru
authors.initials: N
authors.lastname: Koga
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suemune
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013113:Spin Labels
chemical_list: C520181:2-N-tert-butylaminooxyladenosine; D009838:Oligodeoxyribonucleotides; D013113:Spin Labels; D000241:Adenosine