Growth kinetics on oligo- and polysaccharides and promising features of three antioxidative potential probiotic strains.
OBJECTIVE
To determine the antioxidative activity, glutathione production, acid and bile tolerance and carbohydrate preferences of Lactobacillus plantarum LP 1, Streptococcus thermophilus Z 57 and Bifidobacterium lactis B 933.


RESULTS
The intact bacteria exhibited antioxidative capacity against linolenic acid and ascorbate oxidation. The antioxidative activity of cell-free extracts was determined by chemiluminescent assay and agreed with total glutathione content. Superoxide dismutase was negligible in all the strains. Bile and gastric juice resistance was tested in vitro to estimate the transit tolerance in the upper gastrointestinal tract. Bifidobacterium lactis B 933 and L. plantarum LP 1 were more acid tolerant than S. thermophilus Z 57. All the strains were resistant to bile. Among 13 indigestible carbohydrates, galacto-oligosaccharides and fructo-oligosaccharides were utilized by all the strains and did not affect survival in human gastric juice.


CONCLUSIONS
These potential probiotic strains exhibited antioxidative properties and good viability in gastric juice and bile may indicate tolerance to the transit through the upper gastrointestinal tract. Galacto-oligosaccharides and fructo-oligosaccharides are the most appropriate prebiotics to be used in effective synbiotic formulations.


CONCLUSIONS
These results outline promising strains with antioxidative properties. Carbohydrate preferences can be exploited in order to develop synbiotic products.
Zanoni
S
S
Department of Pharmaceutical Sciences, University of Bologna, Bologna, Italy.
Pompei
A
A
Cordisco
L
L
Amaretti
A
A
Rossi
M
M
Matteuzzi
D
D
D000818:Animals; D000975:Antioxidants; D001644:Bifidobacterium / Q000254:growth & development* / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000502:physiology*; D003470:Culture Media; D005750:Gastric Juice / Q000382:microbiology; D005978:Glutathione / Q000096:biosynthesis; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000254:growth & development* / Q000378:metabolism; D019787:Linoleic Acid; D008163:Luminescent Measurements; D050296:Microbial Viability; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000378:metabolism; D019936:Probiotics / Q000378:metabolism; D048249:Streptococcus thermophilus / Q000254:growth & development* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine
D000975:Antioxidants; D003470:Culture Media; D011134:Polysaccharides; D019787:Linoleic Acid; D013482:Superoxide Dismutase; D005978:Glutathione