title: Genetic correlates of morphine withdrawal in 14 inbred mouse strains.
abstract: Male mice from 14 standard inbred strains were exposed to morphine in a sustained released preparation injected subcutaneously. Five hours later withdrawal was precipitated by intraperitoneal injection of naloxone. Mice were tested from 0 to 15 min after naloxone for withdrawal jumping behavior, and then from minute 15-16 for other signs, including boli count, presence of soft stool, lacrimation, "wet dog" shakes, and air chewing. They were also assessed for change in body temperature 17 min after naloxone. Strains differed markedly in the severity of withdrawal for jumping, change in body temperature, and number of fecal boli. Strains also differed in percentage of animals displaying soft stool and air chewing behavior. The other two signs were seen at too low frequency for analysis. Correlations of strain mean withdrawal severity with other responses to morphine and other abused drugs showed that high morphine withdrawal jumping and low change in body temperature were both genetically related to high morphine consumption, but not generally to other measures of morphine withdrawal or morphine sensitivity.
authors.affiliation: Portland Alcohol Research Center, Department of Veterans Affairs, Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR 97239, USA. mettenp@ohsu.edu
authors.forename: Pamela
authors.initials: P
authors.lastname: Metten
authors.forename: John C
authors.initials: JC
authors.lastname: Crabbe
authors.forename: John K
authors.initials: JK
authors.lastname: Belknap
mesh_terms: D000701:Analgesics, Opioid / Q000009:adverse effects*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D009020:Morphine / Q000009:adverse effects*; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D013045:Species Specificity; D013375:Substance Withdrawal Syndrome / Q000235:genetics* / Q000523:psychology*
chemical_list: D000701:Analgesics, Opioid; D009292:Narcotic Antagonists; D009270:Naloxone; D009020:Morphine
references.citation: Annu Rev Pharmacol Toxicol. 1988;28:429-49
references.pmid: 2837979
references.citation: Pharmacogenet Genomics. 2008 Mar;18(3):231-41
references.pmid: 18300945
references.citation: J Neurosci. 2001 Feb 15;21(4):1334-9
references.pmid: 11160404
references.citation: Trends Genet. 2005 Sep;21(9):526-32
references.pmid: 16009447
references.citation: Drug Alcohol Depend. 1993 Apr;32(2):163-7
references.pmid: 8389690
references.citation: J Pharmacol Exp Ther. 1969 May;167(1):1-8
references.pmid: 5814495
references.citation: Behav Neurosci. 1998 Jun;112(3):668-77
references.pmid: 9676982
references.citation: Mamm Genome. 2004 Aug;15(8):610-7
references.pmid: 15457340
references.citation: Trends Pharmacol Sci. 2008 Nov;29(11):537-43
references.pmid: 18774184
references.citation: Behav Genet. 1981 Mar;11(2):103-14
references.pmid: 7271677
references.citation: Genes Brain Behav. 2006 Feb;5(1):1-10
references.pmid: 16436183
references.citation: Pharmacol Biochem Behav. 1999 Jul;63(3):473-9
references.pmid: 10418790
references.citation: Neurosci Lett. 1998 Nov 6;256(2):120-2
references.pmid: 9853718
references.citation: Behav Neurosci. 1994 Feb;108(1):186-95
references.pmid: 8192844
references.citation: BMC Genomics. 2006 Jun 13;7:146
references.pmid: 16772024
references.citation: Alcohol Clin Exp Res. 2003 Mar;27(3):377-87
references.pmid: 12658103
references.citation: Brain Res. 1992 Mar 20;575(2):304-8
references.pmid: 1533339
references.citation: Pharmacol Biochem Behav. 1981 Apr;14(4):575-7
references.pmid: 7195040
references.citation: Nature. 1974 Feb 8;247(5440):398-400
references.pmid: 4856350
references.citation: Mamm Genome. 1998 Dec;9(12):983-90
references.pmid: 9880664
references.citation: Neuroscience. 2002;115(2):463-9
references.pmid: 12421612
references.citation: Pharmacol Biochem Behav. 1998 Feb;59(2):353-60
references.pmid: 9476981
references.citation: Pharmacol Biochem Behav. 2002 Nov;73(4):821-8
references.pmid: 12213527
references.citation: Alcohol Clin Exp Res. 1982 Fall;6(4):446-58
references.pmid: 6758616
references.citation: Behav Pharmacol. 1994 Aug;5(4 And 5):533-547
references.pmid: 11224305
references.citation: Behav Neurosci. 1983 Apr;97(2):280-9
references.pmid: 6849690
references.citation: Psychopharmacology (Berl). 1993;112(2-3):352-8
references.pmid: 7871041
references.citation: Neurosci Biobehav Rev. 2005 Jan;28(8):785-802
references.pmid: 15642621
references.citation: Psychopharmacology (Berl). 1993;112(4):503-10
references.pmid: 7871064
references.citation: Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3380-5
references.pmid: 12612341
references.citation: Genetics. 2007 Jan;175(1):321-33
references.pmid: 17028314
references.citation: Psychopharmacology (Berl). 2002 Jun;161(4):408-16
references.pmid: 12073169
references.citation: Bioinformatics. 2004 Nov 1;20(16):2857-9
references.pmid: 15130929
references.citation: Pharmacogenet Genomics. 2006 Jun;16(6):451-60
references.pmid: 16708053
references.citation: Neurochem Res. 2007 Jul;32(7):1099-112
references.pmid: 17273929
references.citation: Behav Neurosci. 2005 Aug;119(4):911-25
references.pmid: 16187819
references.citation: Mamm Genome. 2001 Jul;12(7):546-53
references.pmid: 11420618
references.citation: Pharmacol Biochem Behav. 1983;18 Suppl 1:541-7
references.pmid: 6685310
references.citation: Drug Alcohol Depend. 2008 Jan 11;93(1-2):185-9
references.pmid: 17961932
references.citation: Genomics. 2005 Nov;86(5):505-17
references.pmid: 16126366
references.citation: J Pharmacol Exp Ther. 1971 Dec;179(3):634-41
references.pmid: 4257807
references.citation: Pharmacol Biochem Behav. 1989 Oct;34(2):241-5
references.pmid: 2622979
references.citation: Gen Pharmacol. 1994 Nov;25(7):1505-10
references.pmid: 7896067
references.citation: Nature. 1972 May 26;237(5352):220-3
references.pmid: 4261153
references.citation: Pharmacol Biochem Behav. 1990 Feb;35(2):311-5
references.pmid: 2320638
references.citation: J Neurosci. 2002 May 1;22(9):3730-8
references.pmid: 11978849
references.citation: Alcohol Clin Exp Res. 1990 Apr;14(2):141-51
references.pmid: 2190477