title: Gene expression profiling of skeletal muscle in exercise-trained and sedentary rats with inborn high and low VO2max.
abstract: The relationship between inborn maximal oxygen uptake (VO(2max)) and skeletal muscle gene expression is unknown. Since low VO(2max) is a strong predictor of cardiovascular mortality, genes related to low VO(2max) might also be involved in cardiovascular disease. To establish the relationship between inborn VO(2max) and gene expression, we performed microarray analysis of the soleus muscle of rats artificially selected for high- and low running capacity (HCR and LCR, respectively). In LCR, a low VO(2max) was accompanied by aggregation of cardiovascular risk factors similar to the metabolic syndrome. Although sedentary HCR were able to maintain a 120% higher running speed at VO(2max) than sedentary LCR, only three transcripts were differentially expressed (FDR <or=0.05) between the groups. Sedentary LCR expressed high levels of a transcript with strong homology to human leucyl-transfer RNA synthetase, of whose overexpression has been associated with a mutation linked to mitochondrial dysfunction. Moreover, we studied exercise-induced alterations in soleus gene expression, since accumulating evidence indicates that long-term endurance training has beneficial effects on the metabolic syndrome. In terms of gene expression, the response to exercise training was more pronounced in HCR than LCR. HCR upregulated several genes associated with lipid metabolism and fatty acid elongation, whereas LCR upregulated only one transcript after exercise training. The results indicate only minor differences in soleus muscle gene expression between sedentary HCR and LCR. However, the inborn level of fitness seems to influence the transcriptional adaption to exercise, as more genes were upregulated after exercise training in HCR than LCR.
authors.affiliation: Department of Circulation and Medical Imaging, Norwegian University of Science and Technology NTNU, Trondheim, Norway.
authors.forename: Anja
authors.initials: A
authors.lastname: Bye
authors.forename: Morten A
authors.initials: MA
authors.lastname: Høydal
authors.forename: Daniele
authors.initials: D
authors.lastname: Catalucci
authors.forename: Mette
authors.initials: M
authors.lastname: Langaas
authors.forename: Ole Johan
authors.initials: OJ
authors.lastname: Kemi
authors.forename: Vidar
authors.initials: V
authors.lastname: Beisvag
authors.forename: Lauren G
authors.initials: LG
authors.lastname: Koch
authors.forename: Steven L
authors.initials: SL
authors.lastname: Britton
authors.forename: Øyvind
authors.initials: Ø
authors.lastname: Ellingsen
authors.forename: Ulrik
authors.initials: U
authors.lastname: Wisløff
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010101:Oxygen Consumption / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats
chemical_list: D007334:Insulin-Like Growth Factor I
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