Determination of amino acid neurotransmitters in human cerebrospinal fluid and saliva by capillary electrophoresis with laser-induced fluorescence detection.
A CE-LIF detection method has been developed to identify and quantitate six amino acid neurotransmitters including glutamic acid, aspartic acid, gamma-aminobutyric acid, glycine, taurine, and glutamine. N-hydroxysuccinimidyl fluorescein-O-acetate, a fluorescein-based dye, was employed for the derivatization of these neurotransmitters prior to CE-LIF analysis. Different parameters which influenced separation and derivatization were optimized in detail. Under optimum conditions, linearity was achieved within concentration ranges of up to three orders of magnitudes for those analytes with correlation coefficients from 0.9989 to 0.9998. The LODs ranged from 0.06 nM to 0.1 nM, and are thus superior or equivalent to those previously reported in the literature using CE-LIF detection. The proposed method has been successfully applied to the determination of amino acid neurotransmitters in biological samples such as human cerebrospinal fluid and saliva with satisfactory recoveries.
Deng
Ying-Hua
YH
Department of Chemistry, Wuhan University, Wuhan, China.
Wang
Hong
H
Zhang
Hua-Shan
HS
D000596:Amino Acids / Q000032:analysis*; D001888:Boric Acids / Q000737:chemistry; D002138:Calibration; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000528:radiation effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D015394:Molecular Structure; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000134:cerebrospinal fluid; D009994:Osmolar Concentration; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013388:Succinimides / Q000737:chemistry / Q000528:radiation effects*; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D000596:Amino Acids; D001888:Boric Acids; D005456:Fluorescent Dyes; C411986:N-hydroxysuccinimidyl fluorescein-O-acetate; D018377:Neurotransmitter Agents; D013388:Succinimides; D013501:Surface-Active Agents