title: Principles of gating.
abstract: Few aspects of flow cytometry are common to every application. Gating principles is one. This unit provides an excellent overview as well as detailed conceptual material on how gating works, when certain types of gating should or should not be applied, and the advantages and disadvantages of various techniques. This unit should be required reading for every technician using a flow cytometer and every graduate student starting out in the area, and is an excellent refresher for those more advanced in the technology. It brings out the historical development of our understanding of gating applications as well as providing a high level of technical accuracy.
authors.affiliation: Coulter Corporation, Miami, Florida, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Wood
mesh_terms: D000818:Animals; D001331:Automation; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D016000:Cluster Analysis; D001330:Electronic Data Processing; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D008027:Light; D008962:Models, Theoretical; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012984:Software