title: Interleukin-10 to tumor necrosis factor-alpha ratio is a predictive biomarker in nonalcoholic fatty liver disease: interleukin-10 to tumor necrosis factor-alpha ratio in steatohepatitis.
abstract: OBJECTIVE
  Fatty liver disease is commonly associated with diabetes mellitus (DM). Insulin resistance (IR) as an investigative biomarker is only concerned with fatty liver that results from DM type 2 associated with metabolic syndrome. Irrespective of IR, DM is generally characterized by overproduction of the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha), whereas action of the latter is modulated by the anti-inflammatory cytokine interleukin-10 (IL-10). The aim of this study was to investigate the efficacy of using TNF-alpha alone or IL-10/TNF-alpha ratio compared to IR, as a promising biomarker for fatty liver assessment in DM. Furthermore, we hypothesized that using garlic as an immunomodulator may decrease TNF-alpha and increase IL-10 production to improve steatohepatitis.
  
  
  METHODS
  DM was induced metabolically by a high-fat diet to bring about IR, or chemically by alloxan, producing insulin deficiency, in male albino rats. Garlic powder was supplemented (15 mg/kg per day) for 3 weeks. Fatty liver was depicted histologically and biochemically (aspartic aminotransferase, alanine aminotransferase, HOMA-IR, TNF-alpha, IL-10, IL-10/TNF-alpha ratio).
  
  
  RESULTS
  We found that, in contrast to obese rats, garlic decreased IL-10/TNF-alpha ratio, despite decreasing TNF-alpha in alloxan diabetic rats in agreement with the histology, which revealed more prominent improvement in the obese group. Moreover, the effect of garlic was not linked to improvement of IR in obese rats.
  
  
  CONCLUSIONS
  We conclude that IL-10/TNF-alpha ratio may be considered as a convenient biomarker for investigation of fatty liver of different grades, apart from being associated with IR, and immunomodulation of this ratio in favor of increasing it may exert significant improvement.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Beni-Suief University, Egypt. drreem30@yahoo.com
authors.forename: Reem M
authors.initials: RM
authors.lastname: Hashem
authors.forename: Mona F
authors.initials: MF
authors.lastname: Mahmoud
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: El-Moselhy
authors.forename: Hala M
authors.initials: HM
authors.lastname: Soliman
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D005234:Fatty Liver / Q000097:blood* / Q000473:pathology; D005737:Garlic; D007333:Insulin Resistance; D016753:Interleukin-10 / Q000097:blood*; D008099:Liver / Q000473:pathology; D008297:Male; D009765:Obesity / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D015415:Biomarkers; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10