title: Increased senescence and reduced functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction.
abstract: OBJECTIVE
  The aim of this study was to investigate the number and functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction.
  
  
  METHODS
  Fetal endothelial progenitor cells were isolated, and counted from 17 women with preeclampsia without intrauterine growth restricion and 30 normal women. Colony-forming assay and differentiation time assay were performed to detect functional activity of the cells. To assess cellular senescence, senescence-associated beta-galactosidase staining was performed for endothelial progenitor cells.
  
  
  RESULTS
  Compared with normal pregnancy, the number of endothelial progenitor cells was significantly lower, differentiation time from endothelial progenitor cell into outgrowing cell was longer, and the number of colonies after differentiation was smaller in preeclampsia (P< .001), respectively. The intensity of senescence-associated beta-galactosidase staining was higher in preeclamptic pregnancy (P < .001).
  
  
  CONCLUSIONS
  The number and functional ability of fetal endothelial progenitor cells from preeclampsia without intrauterine growth restriction are significantly decreased and they are more senescent compared with those of normal pregnancy.
authors.affiliation: Department of Obstetrics and Gynecology, Yonsei University College Health System, Seoul, Korea.
authors.forename: Han-Sung
authors.initials: HS
authors.lastname: Hwang
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Maeng
authors.forename: Yong-Won
authors.initials: YW
authors.lastname: Park
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Koos
authors.forename: Young-Guen
authors.initials: YG
authors.lastname: Kwon
authors.forename: Young-Han
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D016922:Cellular Senescence; D003114:Colony-Forming Units Assay; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005333:Fetus / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D011225:Pre-Eclampsia / Q000473:pathology* / Q000503:physiopathology; D011247:Pregnancy; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D001616:beta-Galactosidase