title: Identification of Tspan9 as a novel platelet tetraspanin and the collagen receptor GPVI as a component of tetraspanin microdomains.
abstract: Platelets are essential for wound healing and inflammatory processes, but can also play a deleterious role by causing heart attack and stroke. Normal platelet activation is dependent on tetraspanins, a superfamily of glycoproteins that function as 'organisers' of cell membranes by recruiting other receptors and signalling proteins into tetraspanin-enriched microdomains. However, our understanding of how tetraspanin microdomains regulate platelets is hindered by the fact that only four of the 33 mammalian tetraspanins have been identified in platelets. This is because of a lack of antibodies to most tetraspanins and difficulties in measuring mRNA, due to low levels in this anucleate cell. To identify potentially platelet-expressed tetraspanins, mRNA was measured in their nucleated progenitor cell, the megakaryocyte, using serial analysis of gene expression and DNA microarrays. Amongst 19 tetraspanins identified in megakaryocytes, Tspan9, a previously uncharacterized tetraspanin, was relatively specific to these cells. Through generating the first Tspan9 antibodies, Tspan9 expression was found to be tightly regulated in platelets. The relative levels of CD9, CD151, Tspan9 and CD63 were 100, 14, 6 and 2 respectively. Since CD9 was expressed at 49000 cell surface copies per platelet, this suggested a copy number of 2800 Tspan9 molecules. Finally, Tspan9 was shown to be a component of tetraspanin microdomains that included the collagen receptor GPVI (glycoprotein VI) and integrin alpha6beta1, but not the von Willebrand receptor GPIbalpha or the integrins alphaIIbbeta3 or alpha2beta1. These findings suggest a role for Tspan9 in regulating platelet function in concert with other platelet tetraspanins and their associated proteins.
authors.affiliation: Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham B152TT, UK.
authors.forename: Majd B
authors.initials: MB
authors.lastname: Protty
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Watkins
authors.forename: Dario
authors.initials: D
authors.lastname: Colombo
authors.forename: Steven G
authors.initials: SG
authors.lastname: Thomas
authors.forename: Victoria L
authors.initials: VL
authors.lastname: Heath
authors.forename: John M J
authors.initials: JM
authors.lastname: Herbert
authors.forename: Roy
authors.initials: R
authors.lastname: Bicknell
authors.forename: Yotis A
authors.initials: YA
authors.lastname: Senis
authors.forename: Leonie K
authors.initials: LK
authors.lastname: Ashman
authors.forename: Fedor
authors.initials: F
authors.lastname: Berditchevski
authors.forename: Willem H
authors.initials: WH
authors.lastname: Ouwehand
authors.forename: Steve P
authors.initials: SP
authors.lastname: Watson
authors.forename: Michael G
authors.initials: MG
authors.lastname: Tomlinson
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D020411:Oligonucleotide Array Sequence Analysis; D010980:Platelet Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D059470:Tetraspanins
chemical_list: D008565:Membrane Proteins; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; C534243:TSPAN9 protein, human; D059470:Tetraspanins; C098341:platelet membrane glycoprotein VI
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