title: Spatiotemporal profiles of field potentials in mouse superior colliculus analyzed by multichannel recording.
abstract: The onset and vector of orienting behaviors, such as saccades, are controlled by commands that descend from a population of neurons in deep layers of the superior colliculus (dSC). In this study, to characterize the role of the collicular local circuitry that generates the spatiotemporal pattern of command activity in the dSC neuronal population, responses evoked by single-pulse electrical stimulation in superficial layers of the superior colliculus (sSC) were analyzed by a 64-channel field potential recording system (planar electrode, 8 x 8 pattern; 150 microm interelectrode spacing) in slices obtained from 16- to 22-d-old mice. A negative field potential with short latency and short duration spatially restricted to the recording sites in sSC was evoked adjacent to the stimulation site. After bath application of 10 mum bicuculline, the same stimulus induced a large negative field response with long duration that spread from sSC to dSC. The dSC potential initially showed a positive response, presumably because of reversal of the negative potential that originated in sSC, and then a long negative response that spread horizontally as far as 1 mm. These responses disappeared after application of an NMDA receptor antagonist, 2-amino-5-phosphonovelarate, indicating that NMDA receptors have an important role in the generation of these responses. Simultaneous whole-cell patch-clamp recordings showed that the long-lasting negative field potentials corresponded to the depolarization accompanying burst spike activity of SC neurons. The present study revealed an extensive excitatory network in the dSC that may contribute to the generation of activity by a large population of neurons that discharge before a saccade.
authors.affiliation: Department of Developmental Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, Japan.
authors.forename: Penphimon
authors.initials: P
authors.lastname: Phongphanphanee
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Kaneda
authors.forename: Tadashi
authors.initials: T
authors.lastname: Isa
mesh_terms: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001640:Bicuculline / Q000494:pharmacology; D004558:Electric Stimulation / Q000379:methods; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods*; D005071:Evoked Potentials / Q000502:physiology* / Q000528:radiation effects; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000502:physiology / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008839:Microelectrodes; D009474:Neurons / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D018408:Patch-Clamp Techniques / Q000379:methods; D013057:Spectrum Analysis; D013477:Superior Colliculi / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D018691:Excitatory Amino Acid Antagonists; D018756:GABA Antagonists; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; C056639:2-amino-5-phosphopentanoic acid; D014633:Valine; D001640:Bicuculline
references.citation: Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6824-7
references.pmid: 17420449
references.citation: Neurosci Res. 2001 Oct;41(2):107-13
references.pmid: 11591438
references.citation: Exp Brain Res. 1978 Nov 15;33(3-4):371-94
references.pmid: 215431
references.citation: Vision Res. 1972 Nov;12(11):1795-808
references.pmid: 4627952
references.citation: Neurosci Res. 2003 May;46(1):73-83
references.pmid: 12725914
references.citation: Brain Res. 1976 Aug 20;113(1):21-34
references.pmid: 821585
references.citation: J Neurophysiol. 1995 Jun;73(6):2334-48
references.pmid: 7666142
references.citation: Prog Brain Res. 1982;57:293-328
references.pmid: 6296921
references.citation: J Neurosci. 2008 Jan 23;28(4):816-27
references.pmid: 18216190
references.citation: J Neurophysiol. 1998 Mar;79(3):1597-602
references.pmid: 9497438
references.citation: J Neurophysiol. 1972 Nov;35(6):915-24
references.pmid: 4631839
references.citation: Exp Brain Res. 1990;79(2):299-312
references.pmid: 2323377
references.citation: J Neurosci Methods. 1999 Oct 30;93(1):61-7
references.pmid: 10598865
references.citation: Brain Res. 2002 Sep 20;950(1-2):279-87
references.pmid: 12231254
references.citation: Trends Neurosci. 1989 Apr;12(4):137-47
references.pmid: 2470171
references.citation: J Neurophysiol. 1972 Jul;35(4):575-86
references.pmid: 4624741
references.citation: J Neurosci. 1997 Nov 1;17(21):8566-79
references.pmid: 9334428
references.citation: Prog Brain Res. 2006;151:321-78
references.pmid: 16221594
references.citation: J Neurosci. 2003 Jul 2;23(13):5854-64
references.pmid: 12843290
references.citation: Physiol Rev. 1986 Jan;66(1):118-71
references.pmid: 3511480
references.citation: J Neurophysiol. 1975 Mar;38(2):219-30
references.pmid: 1092813
references.citation: J Neurophysiol. 1983 May;49(5):1285-301
references.pmid: 6306173
references.citation: J Neurosci. 2000 Feb 1;20(3):RC58
references.pmid: 10648733
references.citation: Annu Rev Neurosci. 1980;3:189-226
references.pmid: 6774653
references.citation: J Neurosci. 2001 Oct 15;21(20):8145-53
references.pmid: 11588187
references.citation: J Neurophysiol. 1993 Aug;70(2):559-75
references.pmid: 8410157
references.citation: J Comp Neurol. 1974 Dec 15;158(4):418-35
references.pmid: 4615112
references.citation: Exp Brain Res. 1984;55(1):172-6
references.pmid: 6086372
references.citation: Curr Opin Neurobiol. 2002 Dec;12(6):668-77
references.pmid: 12490257
references.citation: J Neurosci. 2001 Jan 15;21(2):691-9
references.pmid: 11160448
references.citation: J Neurophysiol. 2005 May;93(5):2898-907
references.pmid: 15601732
references.citation: Trends Cogn Sci. 2006 Aug;10(8):382-90
references.pmid: 16843702
references.citation: J Neurosci. 1998 Oct 15;18(20):8496-504
references.pmid: 9763492
references.citation: J Neurophysiol. 1972 Mar;35(2):187-201
references.pmid: 4623918
references.citation: Science. 1991 Mar 15;251(4999):1358-60
references.pmid: 2003221
references.citation: J Neurophysiol. 2004 Apr;91(4):1706-15
references.pmid: 15010497
references.citation: J Neurophysiol. 1982 Feb;47(2):167-78
references.pmid: 6278101
references.citation: J Neurophysiol. 2004 Dec;92(6):3500-10
references.pmid: 15175376
references.citation: J Neurophysiol. 1993 Aug;70(2):576-89
references.pmid: 8410158
references.citation: Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13299-304
references.pmid: 9371840
references.citation: J Physiol. 2006 Dec 1;577(Pt 2):659-69
references.pmid: 16973709
references.citation: Nature. 1988 Mar 24;332(6162):357-60
references.pmid: 3352733