title: African trypanosomes contain 5-methylcytosine in nuclear DNA.
abstract: It is currently unclear if there are modified DNA bases in Trypanosoma brucei other than J-base. We identify herein a cytosine-5 DNA methyltransferase gene and report the presence and location of 5-methylcytosine in genomic DNA. Our data demonstrate that African trypanosomes contain a functional cytosine DNA methylation pathway.
authors.affiliation: Department of Biology, State University of New York at Geneseo, Geneseo, NY 14454, USA. militello@geneseo.edu
authors.forename: Kevin T
authors.initials: KT
authors.lastname: Militello
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Sangeeta K
authors.initials: SK
authors.lastname: Jayakar
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Pietrasik
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Dupont
authors.forename: Kristi
authors.initials: K
authors.lastname: Dodd
authors.forename: Anthony M
authors.initials: AM
authors.lastname: King
authors.forename: Paul R
authors.initials: PR
authors.lastname: Valenti
mesh_terms: D044503:5-Methylcytosine / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D016054:DNA, Protozoan / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D044503:5-Methylcytosine; D004248:DNA (Cytosine-5-)-Methyltransferases
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