title: Flow rate calibration for absolute cell counting rationale and design.
abstract: There is a need for absolute leukocyte enumeration in the clinical setting, and accurate, reliable (and affordable) technology to determine absolute leukocyte counts has been developed. Such technology includes single platform and dual platform approaches. Derivations of these counts commonly incorporate the addition of a known number of latex microsphere beads to a blood sample, although it has been suggested that the addition of beads to a sample may only be required to act as an internal quality control procedure for assessing the pipetting error. This unit provides the technical details for undertaking flow rate calibration that obviates the need to add reference beads to each sample. It is envisaged that this report will provide the basis for subsequent clinical evaluations of this novel approach.
authors.affiliation: Royal Hallamshire Hospital, Sheffield, United Kingdom.
authors.forename: Clare
authors.initials: C
authors.lastname: Walker
authors.forename: David
authors.initials: D
authors.lastname: Barnett
mesh_terms: D001773:Blood Cells / Q000166:cytology; D002138:Calibration; D005434:Flow Cytometry / Q000379:methods / Q000592:standards; D006801:Humans; D007958:Leukocyte Count / Q000379:methods / Q000592:standards; D007962:Leukocytes / Q000166:cytology* / Q000502:physiology*; D015203:Reproducibility of Results; D012107:Research Design; D012680:Sensitivity and Specificity