title: Evaluation of three methods for measurement of hemoglobin and calculated hemoglobin variables with the ADVIA 120 and ADVIA 2120 systems in goats.
abstract: The present study investigated 3 methods of hemoglobin (Hb) determination in goats using the ADVIA 120 and ADVIA 2120 systems. Ethylenediaminetetraacetic acid anticoagulated caprine blood samples (n = 40 goats) were subjected to Hb determination via the cyanmethemoglobin methods in both instruments and a novel, cyanide-free, colorimetric method with the ADVIA 2120. Statistical analysis of the data included a linear regression, Passing-Bablok regression, and Bland-Altman diagram. Colorimetric Hb results determined with both analyzers had excellent correlation (r = 0.98); however, a mean proportional bias of -19.1% was present in comparison to the reference method. There also was excellent agreement between cellular Hb concentrations when measured with both analyzers (r = 0.96), and the constant bias was close to zero. However, imprecision was higher compared to colorimetric methods. Excellent to fair agreement was evident for all calculated erythrocyte and Hb variables. Because of the excellent correlation between the ADVIA 120 and ADVIA 2120, the cyanide-free method of Hb determination could be used with caprine blood specimens; however, the proportional bias must be considered.
authors.affiliation: Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology, Justus-Liebig University Giessen, Giessen, Germany. Natalie.Bauer@vetmed.uni-giessen.de
authors.forename: Natali
authors.initials: N
authors.lastname: Bauer
authors.forename: Andreas
authors.initials: A
authors.lastname: Moritz
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D001810:Blood Volume / Q000662:veterinary; D004912:Erythrocytes / Q000378:metabolism; D006041:Goats / Q000097:blood*; D006405:Hematology / Q000295:instrumentation* / Q000379:methods; D006454:Hemoglobins / Q000378:metabolism*; D011446:Prospective Studies; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D006454:Hemoglobins