Luminescent and hydrophilic LaF3-polymer nanocomposite for DNA detection.
Luminescent LaF(3)-Ce(3+)/Tb(3+) nanocrystals have been successfully prepared via a simple wet chemical technique. For the next bioapplication, these nanoparticles dispersed in cyclohexane have also been functionalized with poly(St-co-MAA), based on a designed oil-in-water microemulsion system. These polymer-coated nanospheres are water-soluble and bioconjugable. Unlike semiconductor quantum dots, the as-prepared lanthanum fluoride nanocrystals possess non-size-dependent emissions and completely stable photocycles. With functionalized LaF(3) nanospheres as fluorescence probes, a fluorescence method was developed for the rapid quantitative analysis of DNA, due to the quenching effect of fluorescence by the DNA. Under optimum conditions, the fluorescence intensity was proportional to the concentration of the introduced DNA over the range 2.5-35 microg/mL for calf thymus DNA (ctDNA) and 2.5-30 microg/mL for fish sperm DNA (fsDNA), respectively.
Wang
Lun
L
Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China. wanglun@mail.ahnu.edu.cn
Li
Peng
P
Wang
Leyu
L
D000818:Animals; D002138:Calibration; D002417:Cattle; D003102:Colloids; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007811:Lanthanum / Q000737:chemistry*; D049408:Luminescent Agents / Q000138:chemical synthesis* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D011108:Polymers / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry
D003102:Colloids; D049408:Luminescent Agents; D011108:Polymers; D014867:Water; C083668:lanthanum trifluoride; D007811:Lanthanum; D004247:DNA; C427203:calf thymus DNA; D005459:Fluorides