title: Three distinct regions of allelic deletion on chromosome 17 involved in sporadic gastric cancer.
abstract: OBJECTIVE
  Based on the authors' data collected by comparative genomic hybridization (CGH), loss at multiple loci on whole chromosome 17 is believed to be associated with sporadic gastric cancer (GC).
  
  
  METHODS
  In this study, previous results were extended by analyzing further loss of heterozygosity (LOH) at 15 loci along chromosome 17 in 45 sporadic GC patients using fluorescent microsatellite markers and an ABI 377 sequencer/genotyper.
  
  
  RESULTS
  Allelic losses at all 15 markers were observed in most GC patients with the range of 11-61% LOH frequencies. Thirty-three of 45 GC patients (73%) showed LOH at least at one site, 22 at two or more sites. Ten markers were found with over 20% LOH frequencies (5 markers at 17p and 5 markers at 17q), suggesting that frequent allelic losses occur at multiple loci on chromosome 17 in GC. Three distinct regions of deletions were identified: R1 between D17S831-D17S921 at 17p12-13.3 (30cM), R2 between D17S1868-D17S787 at 17q21. 3-22 (11cM) and R3 between D17S785-D17S928 at 17q25.3 (23cM), respectively.
  
  
  CONCLUSIONS
  The observations in this study of the three distinct deleted regions on chromosome 17 may provide some novel abnormalities of potential significance in gastric cancers, suggesting the involvement of TSGs residing in those regions in the genesis of the disease.
authors.affiliation: The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China.
authors.forename: Jingcui
authors.initials: J
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Jingshu
authors.initials: J
authors.lastname: Geng
authors.forename: Jiping
authors.initials: J
authors.lastname: Qi
authors.forename: Songbin
authors.initials: S
authors.lastname: Fu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery