Japanese encephalitis virus differentially modulates the induction of multiple pro-inflammatory mediators in human astrocytoma and astroglioma cell-lines.
Astrocytes become activated in response to many CNS pathologies. The process of astrocyte activation remains rather enigmatic and results in so-called reactive gliosis, a reaction with specific structural and functional characteristics. Astrocytes play a vital role in regulating aspects of inflammation and in the homeostatic maintenance of the CNS. However, the responses of different human astroglial cell-lines in viral encephalitis mediated inflammation are not well documented. We have shown that Japanese encephalitis virus (JEV) infection causes morphological and functional changes in astrocytic cell-lines. We have demonstrated that besides reactive oxygen species (ROS) JEV infection differentially regulated the induction pattern of IL-6, IL-1 beta and IL-8. IP-10, MCP-1, MIG and RANTES secretions in different astroglial cell-lines. The expression of different proteins such as astrocyte-specific glial fibrillary acidic protein (GFAP), the glutamate aspartate transporter/essential amino acid transporter-1 (GLAST/EAAT-1), glutamate transporter-1/essential amino acid transporter-2 (GLT-1/EAAT-2), Ceruloplasmin and Thioredoxin (TRX) expression level also differ in different human astrocyte cell-lines following infection.
Mishra
Manoj Kumar
MK
National Brain Research Centre, Manesar, Haryana 122050, India.
Kumawat
Kanhaiya Lal
KL
Basu
Anirban
A
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000276:immunology* / Q000821:virology; D001254:Astrocytoma / Q000276:immunology; D001921:Brain / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D001932:Brain Neoplasms / Q000276:immunology; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002570:Ceruloplasmin / Q000276:immunology / Q000378:metabolism; D018925:Chemokines / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004664:Encephalitis Virus, Japanese / Q000276:immunology; D004672:Encephalitis, Japanese / Q000276:immunology* / Q000503:physiopathology; D005911:Gliosis / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000276:immunology / Q000378:metabolism; D013879:Thioredoxins / Q000276:immunology / Q000378:metabolism; D050496:Vesicular Glutamate Transport Proteins / Q000276:immunology / Q000378:metabolism
D018925:Chemokines; D016207:Cytokines; D017382:Reactive Oxygen Species; D050496:Vesicular Glutamate Transport Proteins; D013879:Thioredoxins; D002570:Ceruloplasmin