The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens.
The Seeplex TB Detection-2 assay (Rockville, MD) is a nested endpoint PCR for the Mycobacterium tuberculosis complex (MTBC) targets IS6110 and MPB64 that utilizes dual priming oligonucleotide technology. When used to detect the presence of MTBC DNA in formalin-fixed paraffin-embedded tissue specimens, the sensitivity and specificity of this assay is equivalent to a labor-intensive traditional endpoint PCR assay and is more sensitive than a commercial real-time PCR assay.
Drews
Steven J
SJ
Public Health Laboratories Branch, Ministry of Health and Long-Term Care, Toronto, Ontario, Canada. steven.drews@ontario.ca
Eshaghi
AliReza
A
Pyskir
Daria
D
Chedore
Pam
P
Lombos
Ernesto
E
Broukhanski
George
G
Higgins
Rachel
R
Fisman
David N
DN
Blair
Joanne
J
Jamieson
Frances
F
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