Phylogenetic studies of Gordonia species based on gyrB and secA1 gene analyses.
Phylogenetic relations within the genus Gordonia were analyzed using partial gyrB and secA1 gene sequences of 23 type species in comparison with those of 16S rRNA gene. The gyrB and secA1 phylogenies showed agreement with that constructed using 16S rRNA gene sequences. The degrees of divergence of the gyrB and secA1 genes were approximately 3.4 and 1.7 times greater, respectively, than that of 16S rRNA gene. The gyrB gene showed more discriminatory power than either the secA1 or 16S rRNA gene, facilitating clear differentiation of any two Gordonia species using gyrB gene analysis. Our data indicate that gyrB and secA1 gene sequences are useful as markers for phylogenetic study and identification at the species level of the genus Gordonia.
Kang
Yingqian
Y
Medical Mycology Research Center, Chiba University, I-8-1, Inohana, Chuo-ku, Chiba, 260-8673, Japan.
Takeda
Kenjiro
K
Yazawa
Katsukiyo
K
Mikami
Yuzuru
Y
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D015373:Bacterial Typing Techniques; D017124:Conserved Sequence; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial; D033601:Gordonia Bacterium / Q000145:classification* / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D012336:RNA, Ribosomal, 16S; D000069816:SEC Translocation Channels; D000251:Adenosine Triphosphatases; D027081:DNA Gyrase; D000081416:SecA Proteins
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