title: Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin.
abstract: BACKGROUND
  Previous studies demonstrated the EGF-targeted phagemid particles carrying siRNA against Akt could be expressed efficiently in the presence of hydroxycamptothecin (HCPT). However, no significant cell growth inhibition was obtained. This study was to further investigate whether the EGF-targeted phagemid particles carrying siRNA would be a promising tool for anti-cancer siRNA delivery.
  
  
  RESULTS
  We found that pSi4.1-siFAK phagemid particles could significantly inhibit the expression of focal adhesion kinase in the HCPT-treated cells. Moreover, we also observed that the particles could potently suppress cell growth and cell invasion.
  
  
  CONCLUSIONS
  These results indicated that EGF-targeted phagemid particles might be a promising tool for anti-cancer siRNA delivery in the presence of HCPT.
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, PR China. caixm06@gmail.com
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Cai
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Xie
authors.forename: Ming-Zhu
authors.initials: MZ
authors.lastname: Liu
authors.forename: Xi-Liang
authors.initials: XL
authors.lastname: Zha
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D002166:Camptothecin / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D003131:Combined Modality Therapy; D004815:Epidermal Growth Factor / Q000235:genetics; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000473:pathology / Q000628:therapy*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D034741:RNA, Small Interfering; D004815:Epidermal Growth Factor; C028098:10-hydroxycamptothecin; D051416:Focal Adhesion Protein-Tyrosine Kinases; D002166:Camptothecin
references.citation: Biotechniques. 2006 Dec;41(6):706-7
references.pmid: 17191613
references.citation: Cancer Gene Ther. 1998 Nov-Dec;5(6):357-64
references.pmid: 9917090
references.citation: Lung Cancer. 2006 Sep;53(3):263-71
references.pmid: 16842883
references.citation: Biochem Biophys Res Commun. 1999 Nov 2;264(3):921-8
references.pmid: 10544031
references.citation: FASEB J. 1999 Apr;13(6):727-34
references.pmid: 10094933
references.citation: J Mol Biol. 1999 Apr 30;288(2):203-11
references.pmid: 10329137
references.citation: Anticancer Res. 2004 Nov-Dec;24(6):3899-905
references.pmid: 15736429
references.citation: Biotechniques. 2005 Oct;39(4):493-7
references.pmid: 16235561
references.citation: Int J Cancer. 2005 Dec 20;117(6):905-12
references.pmid: 15986432
references.citation: Biotechnol Prog. 2006 May-Jun;22(3):626-30
references.pmid: 16739942
references.citation: Oncogene. 2006 Jul 27;25(32):4429-40
references.pmid: 16547501
references.citation: Mol Cell Biochem. 2000 Apr;207(1-2):49-55
references.pmid: 10888226
references.citation: Nat Biotechnol. 2001 Jan;19(1):75-8
references.pmid: 11135557
references.citation: Virology. 2001 Mar 30;282(1):102-12
references.pmid: 11259194
references.citation: Cancer Res. 2002 Feb 15;62(4):977-81
references.pmid: 11861367
references.citation: Hum Pathol. 2003 Mar;34(3):240-5
references.pmid: 12673558
references.citation: Am J Pathol. 2004 Oct;165(4):1087-95
references.pmid: 15466376
references.citation: Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10879-84
references.pmid: 9380728
references.citation: Head Neck. 1998 Dec;20(8):745-52
references.pmid: 9790298
references.citation: Acta Pharmacol Sin. 2008 Apr;29(4):437-42
references.pmid: 18358089
references.citation: Hum Gene Ther. 1998 Nov 1;9(16):2393-9
references.pmid: 9829538