Light scatter: detection and usage.
Light scatter is one of the most basic of measurements made in flow cytometry. This unit presents a short commentary on what light scatter is and how it is measured. First, the author describes the basic principles of the scattering of light from small particles such as cells. This is followed by a discussion of methods for detecting scattered light in a flow cytometer. The unit ends with a brief survey showing how light scattering is used in a broad range of flow cytometry applications.
Salzman
G C
GC
Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
D002138:Calibration; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D008027:Light; D012542:Scattering, Radiation