title: Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor beta in HEK293 cells.
abstract: Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor beta, (PPARbeta). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARbeta in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARbeta. Furthermore, PPARbeta also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.
authors.affiliation: Department of Biology, College of Life Science and Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Hae Jin
authors.initials: HJ
authors.lastname: Song
authors.forename: Joongkyu
authors.initials: J
authors.lastname: Park
authors.forename: Su Ryeon
authors.initials: SR
authors.lastname: Seo
authors.forename: Jongsun
authors.initials: J
authors.lastname: Kim
authors.forename: Seung R
authors.initials: SR
authors.lastname: Paik
authors.forename: Kwang Chul
authors.initials: KC
authors.lastname: Chung
mesh_terms: D002460:Cell Line; D015723:Gene Library; D006801:Humans; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D048068:PPAR-beta / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
chemical_list: D008565:Membrane Proteins; D018832:Molecular Chaperones; D009124:Muscle Proteins; D048068:PPAR-beta; C410584:PSMG1 protein, human; D011480:Protease Inhibitors