title: Sulphurous mineral water oral therapy: effects on erythrocyte metabolism.
abstract: The ingestion of water containing hydrogen sulphide (H(2)S) is common in spring sulphurous mineral water (SMW) therapy. We hypothesized that observed detrimental effects are related to the alteration of erythrocytes metabolism caused by H(2)S. To verify our hypothesis, we treated 20 healthy volunteers with SMW and evidenced an increase of methemoglobin concentration, an inhibition of both erythrocyte glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glucose-6-phosphate dehydrogenase (G6PDH) activities. To investigate the mechanism of H(2)S effect on GAPDH activity, an in vitro study was performed by incubating both erythrocytes from 12 healthy volunteers and purified GAPDH with buffered [(35)S]-H(2)S labelled sulphurous water. The interaction between H(2)S and NAD(+)(H), was also investigated. The results indicate that a direct reaction between GAPDH and H(2)S does not occur and the observed decrease of GAPDH activity is to ascribe to the reaction between H(2)S and NAD(+)(H). This may lead to GAPDH inhibition by two ways, namely (i) cellular NAD(+)(H) reduced availability and (ii) catalytic site blockage. In conclusion, our results show that among the detrimental effects of SMW administration are erythrocyte GAPDH and G6PDH activity inhibition and increased methemoglobin concentration. A mechanism to explain the occurrence of these biochemical events is also proposed.
authors.affiliation: Istituto di Chimica Biologica G. Fornaini, Università degli Studi di Urbino, Urbino 61029, Italy. mc.albertini@hotmail.it
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Albertini
authors.forename: Laura
authors.initials: L
authors.lastname: Teodori
authors.forename: Augusto
authors.initials: A
authors.lastname: Accorsi
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Soukri
authors.forename: Luigi
authors.initials: L
authors.lastname: Campanella
authors.forename: Francesco
authors.initials: F
authors.lastname: Baldoni
authors.forename: Marina
authors.initials: M
authors.lastname: Dachà
mesh_terms: D000328:Adult; D004912:Erythrocytes / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry* / Q000494:pharmacology*; D008900:Mineral Waters / Q000032:analysis*; D015394:Molecular Structure; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D009536:Niacinamide / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D013997:Time Factors
chemical_list: D008900:Mineral Waters; D009243:NAD; D009536:Niacinamide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D006862:Hydrogen Sulfide