Evaluating the insecticidal genes and their expressed products in Bacillus thuringiensis strains by combining PCR with mass spectrometry.
By a combination of PCR and mass spectrometry, a total of five cry genes (cry1Aa, cry1Ac, cry2Aa, cry2Ab, and cry1Ia) were detected in genomic DNA from the wild-type Bacillus thuringiensis strain 4.0718, and three protoxins (Cry1Aa, Cry1Ac, and Cry2Aa) were identified in the strain's parasporal crystals. These results indicated that this complementary method may be useful in evaluating B. thuringiensis strains at both the gene and protein levels.
Sun
Yunjun
Y
College of Life Science, Hunan Normal University, Changsha 410081, China.
Fu
Zujiao
Z
Ding
Xuezhi
X
Xia
Liqiu
L
D001413:Bacillus thuringiensis / Q000737:chemistry* / Q000235:genetics*; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics*; D001427:Bacterial Toxins / Q000032:analysis* / Q000235:genetics*; D013058:Mass Spectrometry; D016133:Polymerase Chain Reaction
D001426:Bacterial Proteins; D001427:Bacterial Toxins
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