Strategies for shotgun identification of integral membrane proteins by tandem mass spectrometry.
Integral membrane proteins (IMPs) are difficult to identify, mainly for two reasons: the hydrophobicity of IMPs and their low abundance. Sample preparation is a key component in the large-scale identification of IMPs. In this review, we survey strategies for shotgun identification of IMPs by MS/MS. We will discuss enrichment, solubilization, separation, and digestion of IMPs, and data analysis for membrane proteomics.
Lu
Bingwen
B
Department of Chemical Physiology, SR-11, The Scripps Research Institute, La Jolla, CA 92037, USA.
McClatchy
Daniel B
DB
Kim
Jin Young
JY
Yates
John R
JR
D000818:Animals; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D008565:Membrane Proteins