Coxsackievirus B4 uses autophagy for replication after calpain activation in rat primary neurons.
Coxsackievirus is the most important cause of meningitis and encephalitis in infants; an infection is sometimes fatal or may lead to neurodevelopmental defects. Here, we show that coxsackievirus B4 (CVB4) induces an autophagy pathway for replication in rat primary neurons. Notably, calpain inhibitors reduce autophagosome formation. Conversely, the inhibition of the autophagy pathway with 3-methyladenine inhibits calpain activation. This work reveals, for the first time, that calpain is essential for the autophagy pathway and viral replication in CVB4-infected neurons.
Yoon
Seung Yong
SY
Department of Anatomy and Cell Biology, University of Ulsan College of Medicine, 388-1 PoongNap-Dong, SongPa-Gu, Seoul 138-736, South Korea.
Ha
Young Eun
YE
Choi
Jung Eun
JE
Ahn
Jeonghyun
J
Lee
Heuiran
H
Kweon
Hee-Seok
HS
Lee
Jee-Young
JY
Kim
Dong Hou
DH
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D001343:Autophagy; D002154:Calpain / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000821:virology; D029822:Enterovirus B, Human / Q000502:physiology*; D009474:Neurons / Q000821:virology*; D051381:Rats; D014779:Virus Replication
C025946:3-methyladenine; D002154:Calpain; D000225:Adenine
Obstet Gynecol. 2001 Dec;98(6):1019-26
11755547
J Cell Biol. 2006 Nov 20;175(4):595-605
17101693
Curr Opin Immunol. 2006 Aug;18(4):375-82
16782319
Nat Rev Mol Cell Biol. 2007 Sep;8(9):741-52
17717517
Mol Biol Cell. 2004 Mar;15(3):1101-11
14699058
Neurosci Lett. 2002 Jul 5;326(3):175-8
12095650
PLoS Biol. 2005 May;3(5):e156
15884975
Neurobiol Dis. 2006 May;22(2):435-44
16480887
J Biol Chem. 2000 Jan 14;275(2):992-8
10625637
Nat Cell Biol. 2006 Oct;8(10):1124-32
16998475
J Virol. 2007 Oct;81(19):10815-7
17670838
J Virol. 2008 Feb;82(3):1581-90
18032503
Prog Med Virol. 1973;15:42-62
4572439