Metagenomic screening for bleomycin resistance genes.
A metagenomic library of activated sludge was screened for bleomycin resistance genes. Two genes were identified that differed greatly from each other, from the genes of bleomycin-producing actinomycetes, and from those of clinical isolates. Therefore, the nonclinical environment is a rich reservoir of new resistance elements, and metagenomics can be used to sample the resistome rapidly.
Mori
Toshio
T
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Tsukuba, Ibaraki 305-8566, Japan.
Mizuta
Shiori
S
Suenaga
Hikaru
H
Miyazaki
Kentaro
K
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001419:Bacteria / Q000235:genetics*; D001761:Bleomycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D017385:Sequence Homology; D012722:Sewage / Q000382:microbiology*
D000903:Antibiotics, Antineoplastic; D012722:Sewage; D001761:Bleomycin
FEBS Lett. 1988 Mar 28;230(1-2):171-5
2450783
Microbiol Mol Biol Rev. 2004 Dec;68(4):669-85
15590779
Res Microbiol. 2001 Jun;152(5):421-30
11446510
J Antibiot (Tokyo). 1969 Sep;22(9):446-8
4982665
Gene. 1984 Dec;32(1-2):225-33
6085062
J Nat Prod. 2002 Mar;65(3):422-31
11908996
FEBS Lett. 1999 Jan 8;442(1):34-8
9923599
Comput Appl Biosci. 1996 Aug;12(4):357-8
8902363
Mol Biosyst. 2007 Jan;3(1):60-74
17216057
Plasmid. 1987 Jan;17(1):46-53
2437604
Science. 2006 Jan 20;311(5759):374-7
16424339
Environ Microbiol. 2007 Sep;9(9):2289-97
17686025
Gene. 1992 Oct 12;120(1):99-103
1327971
Curr Opin Microbiol. 2007 Oct;10(5):481-9
17951101
Environ Microbiol. 2004 Sep;6(9):981-9
15305923
J Antibiot (Tokyo). 1966 Sep;19(5):200-9
5953301
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
9396791