title: Biotin sulfone as a new tool for synthetic oligosaccharide immobilization: application to multiple analysis profiling and surface plasmonic analysis of anti-Candida albicans antibody reactivity against alpha and beta (1-->2) oligomannosides.
abstract: As a part of our glycoantigen synthetic program for diagnosis and basic analysis of yeast-related pathogenic mechanisms, a library of 1-->2 oligomannosides suitable for immunoanalysis was prepared. The use of biotin sulfone, an oxidized form of biotin, offers a convenient solution for both oligosaccharide synthesis and immobilization on microspheres and surface plasmon resonance sensors. The application of this new strategy for the analysis of anti- Candida albicans antibody response through multiple-analyte profiling technology (Luminex) and with surface plasmonic analysis using biotin tagged synthetic oligosaccharides on avidin coated surfaces was validated using monoclonal antibodies.
authors.affiliation: Ecole Normale Supérieure, Département de Chimie, UMR CNRS 8642, Paris, France.
authors.forename: Mayeul
authors.initials: M
authors.lastname: Collot
authors.forename: Boualem
authors.initials: B
authors.lastname: Sendid
authors.forename: Aurélie
authors.initials: A
authors.lastname: Fievez
authors.forename: Camille
authors.initials: C
authors.lastname: Savaux
authors.forename: Annie
authors.initials: A
authors.lastname: Standaert-Vitse
authors.forename: Marc
authors.initials: M
authors.lastname: Tabouret
authors.forename: Anne Sophie
authors.initials: AS
authors.lastname: Drucbert
authors.forename: Pierre Marie
authors.initials: PM
authors.lastname: Danzé
authors.forename: Daniel
authors.initials: D
authors.lastname: Poulain
authors.forename: Jean-Maurice
authors.initials: JM
authors.lastname: Mallet
mesh_terms: D000908:Antibodies, Fungal / Q000032:analysis* / Q000737:chemistry; D001360:Avidin / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D002176:Candida albicans / Q000276:immunology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007118:Immunoassay; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013450:Sulfones / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
chemical_list: D000908:Antibodies, Fungal; D009844:Oligosaccharides; D013450:Sulfones; C028569:oligomannoside; D001360:Avidin; C104349:biotin sulfone; D001710:Biotin