title: Association between Val/Leu(247) polymorphism of apolipoprotein H and cerebral infarction in a Chinese population.
abstract: BACKGROUND
  Antiphospholipid antibodies (aPL) are considered to be a cause of an acquired hypercoagulable state leading to cerebral infarction (CI). Apolipoprtein H (apoH) is an important target antigen for aPL and thus apoH polymorphisms may influence aPL production and the development of CI. The purpose of this study was to identify associations between the Val/Leu(247) polymorphism of apoH gene and CI in a Chinese cohort.
  
  
  METHODS
  This study comprised 130 CI patients and 100 healthy control subjects. Polymorphism assignment was determined by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique and DNA sequencing. The presence of aPL was detected by ELISA utilizing irradiated ELISA plates.
  
  
  RESULTS
  Our results demonstrated an association between the Val/Leu(247) polymorphism of apoH gene and aPL in CI patients. The frequency of V allele was significantly higher in aPL-positive CI patients compared with control group (chi(2) = 6.864, P < 0.05). VL genotype frequency was also significantly higher in aPL-positive CI group compared with control group (chi(2) = 13.879, P < 0.05) and aPL-negative CI group (chi(2) = 5.567, P < 0.05).
  
  
  CONCLUSIONS
  The Val(247) allele of apoH gene is significantly associated with the presence of aPL in Chinese patients with CI.
authors.affiliation: Institute of Neurology, Xiangya Hospital, Central South University, Hunan, People's Republic of China. xiajian1216@sina.com
authors.forename: Jian
authors.initials: J
authors.lastname: Xia
authors.forename: Mei
authors.initials: M
authors.lastname: Yuan
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Xu
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Du
authors.forename: Yun-hai
authors.initials: YH
authors.lastname: Liu
authors.forename: Qi-dong
authors.initials: QD
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D017152:Antibodies, Antiphospholipid / Q000097:blood*; D044466:Asian People; D016022:Case-Control Studies; D002544:Cerebral Infarction / Q000097:blood / Q000208:ethnology / Q000235:genetics*; D002681:China / Q000453:epidemiology; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012307:Risk Factors; D053482:beta 2-Glycoprotein I / Q000235:genetics*
chemical_list: D017152:Antibodies, Antiphospholipid; D053482:beta 2-Glycoprotein I
references.citation: Neurology. 2001;57(5 Suppl 2):S24-30
references.pmid: 11552051
references.citation: Ann Hum Genet. 2004 Jul;68(Pt 4):285-99
references.pmid: 15225155
references.citation: J Exp Med. 1994 Feb 1;179(2):457-62
references.pmid: 7507506
references.citation: Arthritis Rheum. 2005 Jan;52(1):212-8
references.pmid: 15641049
references.citation: Hum Genet. 1997 Jul;100(1):57-62
references.pmid: 9225969
references.citation: Thromb Haemost. 1998 Nov;80(5):791-7
references.pmid: 9843173
references.citation: Lupus. 2006;15(4):218-22
references.pmid: 16686261
references.citation: Autoimmun Rev. 2007 Sep;6(8):547-52
references.pmid: 17854747
references.citation: Cerebrovasc Dis. 2005;20 Suppl 2:75-83
references.pmid: 16327256
references.citation: Neurology. 1993 Oct;43(10):2069-73
references.pmid: 8413969
references.citation: Cerebrovasc Dis. 2002;13 Suppl 1:7-11
references.pmid: 11803181
references.citation: Br J Haematol. 2003 Mar;120(6):1066-72
references.pmid: 12648080
references.citation: Neurology. 2001 Apr 24;56(8):997-1008
references.pmid: 11339244
references.citation: Hokkaido Igaku Zasshi. 2001 Sep;76(5):289-98
references.pmid: 11593753
references.citation: Thromb Haemost. 1995 Oct;74(4):1185-90
references.pmid: 8560433
references.citation: Thromb Res. 2004;114(5-6):489-99
references.pmid: 15507283
references.citation: Arthritis Rheum. 2003 Feb;48(2):471-4
references.pmid: 12571857
references.citation: Arthritis Rheum. 1999 Aug;42(8):1655-61
references.pmid: 10446865
references.citation: Cerebrovasc Dis. 2005;20 Suppl 2:53-67
references.pmid: 16327254
references.citation: Rheumatology (Oxford). 1999 Aug;38(8):721-3
references.pmid: 10501418
references.citation: Rom J Intern Med. 2007;45(2):201-4
references.pmid: 18333375
references.citation: Eur J Biochem. 2003 Jan;270(2):230-8
references.pmid: 12605674
references.citation: Clin Rheumatol. 2007 Mar;26(3):302-7
references.pmid: 16724168
references.citation: Eur J Biochem. 1999 Jan;259(1-2):435-40
references.pmid: 9914524
references.citation: Prostaglandins Other Lipid Mediat. 1999 Jul;57(5-6):351-9
references.pmid: 10480489
references.citation: Intern Med. 2006;45(17):1017-8
references.pmid: 17016003
references.citation: Lupus. 2005;14(6):440-4
references.pmid: 16038107