title: Manipulation of major membrane lipid synthesis and its effects on sporulation in Saccharomyces cerevisiae.
abstract: During the sporulation process of Saccharomyces cerevisiae, meiotic progression is accompanied by de novo formation of the prospore membrane inside the cell. However, it remains to be determined whether certain species of lipids are required for spore formation in yeast. In this study, we analyzed the requirement of the synthesis of phosphatidylethanolamine (PE), phosphatidylcholine (PC), and ergosterol for spore formation using strains in which the synthesis of these lipids can be controlled. When synthesis of PE and PC was repressed, sporulation efficiency decreased. This suggests that synthesis of these phospholipids is vital to proper sporulation. In addition, sporulation was also impaired in cells with a lowered sterol content, raising the possibility that sterol content is also important for spore formation.
authors.affiliation: Department of Biotechnology, The University of Tokyo, Tokyo, Japan.
authors.forename: Lan
authors.initials: L
authors.lastname: Deng
authors.forename: Jumpei
authors.initials: J
authors.lastname: Nagasawa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ono
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Toru
authors.initials: T
authors.lastname: Kakihara
authors.forename: Ryouichi
authors.initials: R
authors.lastname: Fukuda
authors.forename: Akinori
authors.initials: A
authors.lastname: Ohta
mesh_terms: D004875:Ergosterol / Q000096:biosynthesis*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008540:Meiosis; D008563:Membrane Lipids / Q000096:biosynthesis*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013172:Spores, Fungal / Q000235:genetics* / Q000378:metabolism
chemical_list: D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine; D004875:Ergosterol