title: A novel biomimetic olfactory-based biosensor for single olfactory sensory neuron monitoring.
abstract: This paper presents a novel biomimetic olfactory biosensor for the study of olfactory transduction mechanisms on the basis of light addressable potentiometric sensor (LAPS), in which rat olfactory sensory neurons (OSNs) are used as sensing elements. Rat OSNs are cultured on the surface of LAPS chip. To validate the origin of the electrical signals recorded by LAPS, the inhibitory effect of MDL12330A to the olfactory signals of OSNs is tested, which is the specific inhibitor of adenylyl cyclase. The enhancive effect of LY294002 to the responses of OSNs is also investigated, which is the specific inhibitor of phosphatidylinositol 3-kinase (PI3K). The results show that this hybrid biosensor can record the responses of OSNs to odours efficiently in a non-invasive way for a long term, and the responses can be inhibited by MDL12330A and enhanced by LY294002. All these results demonstrate that this hybrid biosensor can be used to monitor electrophysiology of OSNs in a non-invasive way and suggest it could be a promising tool for the study of olfactory transduction mechanisms.
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Zheda Road No. 38, Hangzhou 310027, PR China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wu
authors.forename: Peihua
authors.initials: P
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Liu
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zong
authors.forename: Hua
authors.initials: H
authors.lastname: Cai
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D032701:Biomimetics / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation; D002478:Cells, Cultured; D002867:Chromones / Q000008:administration & dosage*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D009025:Morpholines / Q000008:administration & dosage*; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000502:physiology*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012903:Smell / Q000502:physiology*
chemical_list: D002867:Chromones; D009025:Morpholines; D000081082:Phosphoinositide-3 Kinase Inhibitors; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one