title: Digital fluorescence microscopy.
abstract: Fluorescence microscopy is a valuable tool for biomedical research, providing good sensitivity and high multiplicity. Specialized techniques (anisotropy measurement, resonance energy transfer) can provide information on cell spatial arrangement or label microenvironment. The unit discusses camera selection strategy, microscope hardware, and image acquisition and processing.
authors.affiliation: Leiden University, Leiden, The Netherlands.
authors.forename: H J
authors.initials: HJ
authors.lastname: Tanke
mesh_terms: D000818:Animals; D016880:Anisotropy; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D012984:Software