title: Lipophilic DNA-conjugates: DNA controlled assembly of liposomes.
abstract: DNA detection systems based on encoded solid particles have been reported but require often tedious and not generally applicable surface chemistry. In the present study a system comprised of a lipid-modified DNA probe sequence and unmodified DNA target sequences is used to non-covalently assemble liposomes. The process results in large liposome aggregates with dramatically different optical properties compared to individual liposomes in solution. The presented method enables fast and easy detection of target polynucleotides. Furthermore, the system displays remarkably sharp thermal transitions which enable detection of single nucleotide polymorphisms with greatly enhanced resolution compared to fluorescence based methods.
authors.affiliation: University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
authors.forename: Ulla
authors.initials: U
authors.lastname: Jakobsen
authors.forename: Stefan
authors.initials: S
authors.lastname: Vogel
mesh_terms: D043844:Crown Ethers / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide
chemical_list: D043844:Crown Ethers; D008081:Liposomes; D004247:DNA