[Study on the ingestion of folate and polymorphism of MTHFR C677T with esophageal cancer in Xinjiang Kazakh].
OBJECTIVE
To study the relationship between esophageal cancer (EC) and the ingestion of folate, methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphisms in Kazakh area, Xinjiang.


METHODS
A 1:2 matched case-control study was adopted. 120 cases diagnosed as esophageal cancer were collected with 240 population-based and hospital-based controls were selected by the same sex, same nationality and each pair's ages were permitted to differ within 5 years. MTHFR genotypes were detected by polymeerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the conditional logistic regression model was performed in this study.


RESULTS
Data showed that the ingestion of folate was related to EC in Kazakh (chi2 = 7.868, nu = 1, P < 0.01) with OR: 0.519 (95% CI:0.329-0.821) while more folate intake appeared to be the protective factor of EC in Kazakh. The MTHFR C677T genotype frequencies of EC group was different from the control group (chi2 = 15.823, nu = 1, P < 0.01). The individuals with 677CT, TT genotype had a 2.613-fold (95% CI: 1.628-4.194) increased risk of developing EC, compared to those who had 677CC genotype. Data from Interaction Analysis showed that more folate intake could reduce the incidence of esophageal cancer to the individuals who carried the MTHFR 677CT or TT genotypes.


RESULTS
from multivariate conditional logistic regression analysis showed that: unsanitary drinking water, irregular eating, prefer eating peppery food, engorgement, crusted rice or wheat, having history of stomach or esophagus illness, carrying MTHFR 677CT or TT genotypes were risk factors of esophageal cancer while taking in more folate was the protective factor of EC.


CONCLUSIONS
Lacking of folate intake were mainly risk factor and the polymorphisms of MTHFRC677T gene were susceptibility factor of esophageal cancer in Kazakh in Xinjiang.
Qin
Jiang-mei
JM
Laboratory of Xinjiang Endemic and Ethnic Disease, Medical of College, Shihezi University, Shihezi 832002, China.
Wang
Xiu-mei
XM
Chen
Bo
B
Yang
Lei
L
Li
Feng
F
He
Ling
L
Liao
Pei-hua
PH
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China; D004938:Esophageal Neoplasms / Q000453:epidemiology* / Q000209:etiology / Q000235:genetics*; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage*; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics
D005492:Folic Acid; D042965:Methylenetetrahydrofolate Reductase (NADPH2)