Comparative contents of mRNAs of sex steroid receptors and enzymes of their metabolism in arterial walls of men.
The potential role of estrogens in regulation of metabolism in arteries of men was studied. Contents of mRNAs of sex hormone receptors, of some enzymes of their metabolism, and of some potential markers of the hormone effects were determined by real-time polymerase chain reaction in fragments of 18-54-year-old men's large arteries with and without atherosclerotic lesions. Contents of estrogen receptor alpha (ERalpha) and transferrin receptor mRNAs were significantly different in undamaged fragments of the aorta and of the carotid and coronary arteries. Contents of some mRNAs in the carotid artery and aorta were found to correlate, which suggested a similarly directed regulation of their expressions. The levels of ERalpha and aromatase mRNAs negatively correlated with the blood plasma concentration of estradiol. Levels of steroid sulfatase and aromatase mRNAs were lower and the level of estrogen sulfotransferase mRNA was higher in blood vessel fragments with atherosclerotic lesions than in undamaged fragments. It is suggested that large arteries should be different in sensitivity to estrogens and that atherosclerotic lesions could lead to local suppression of the effect of estrogen on the cells of arteries.
Shchelkunova
T A
TA
Biological Faculty, Lomonosov Moscow State University, Moscow, Russia.
Morozov
I A
IA
Rubtsov
P M
PM
Samokhodskaya
L M
LM
Kireev
R A
RA
Andrianova
I V
IV
Orekhov
A N
AN
Smirnov
A N
AN
D000293:Adolescent; D000328:Adult; D001011:Aorta / Q000033:anatomy & histology / Q000378:metabolism; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D001158:Arteries / Q000033:anatomy & histology / Q000378:metabolism; D002339:Carotid Arteries / Q000033:anatomy & histology / Q000378:metabolism; D003331:Coronary Vessels / Q000033:anatomy & histology / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D011944:Receptors, Androgen / Q000235:genetics* / Q000378:metabolism; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism; D043266:Steryl-Sulfatase / Q000235:genetics* / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D011944:Receptors, Androgen; D011990:Receptors, Transferrin; D019010:Vascular Cell Adhesion Molecule-1; D013739:Testosterone; D004958:Estradiol; D001141:Aromatase; D043266:Steryl-Sulfatase