Large ventral lateral neurons modulate arousal and sleep in Drosophila.
BACKGROUND
Large ventral lateral clock neurons (lLNvs) exhibit higher daytime-light-driven spontaneous action-potential firing rates in Drosophila, coinciding with wakefulness and locomotor-activity behavior. To determine whether the lLNvs are involved in arousal and sleep/wake behavior, we examined the effects of altered electrical excitation of the LNvs.


RESULTS
LNv-hyperexcited flies reverse the normal day-night firing pattern, showing higher lLNv firing rates at night and pigment-dispersing-factor-mediated enhancement of nocturnal locomotor-activity behavior and reduced quantity and quality of sleep. lLNv hyperexcitation impairs sensory arousal, as shown by physiological and behavioral assays. lLNv-hyperexcited flies lacking sLNvs exhibit robust hyperexcitation-induced increases in nocturnal behavior, suggesting that the sLNvs are not essential for mediation of arousal.


CONCLUSIONS
Light-activated lLNvs modulate behavioral arousal and sleep in Drosophila.
Sheeba
Vasu
V
Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA.
Fogle
Keri J
KJ
Kaneko
Maki
M
Rashid
Saima
S
Chou
Yu-Ting
YT
Sharma
Vijay K
VK
Holmes
Todd C
TC
D000200:Action Potentials; D000818:Animals; D001143:Arousal / Q000502:physiology*; D001522:Behavior, Animal / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology; D004330:Drosophila / Q000502:physiology*; D008027:Light; D008297:Male; D009043:Motor Activity; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D012890:Sleep / Q000502:physiology*; D014851:Wakefulness
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