title: Sorting of bacteria.
abstract: This unit provides detailed instructions for the sorting of organisms using the cell sorter. These techniques are very valuable for establishing pure cultures of organisms, for example those expressing GFP. The unit provides information on the culture, sorting, reculture, and verification of the sort purity and GFP expression. For those venturing into the area of bacterial sorting, this unit is a must.
authors.affiliation: Iowa State University, Ames, Iowa, USA.
authors.forename: K R
authors.initials: KR
authors.lastname: Harkins
mesh_terms: D001419:Bacteria / Q000166:cytology*; D001431:Bacteriological Techniques; D002469:Cell Separation / Q000379:methods*; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000166:cytology; D005434:Flow Cytometry / Q000379:methods; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D049452:Green Fluorescent Proteins