New triarylamine-based far-red DNA stainers with high two-photon absorption properties.
A series of red emitting vinyl-triphenyamines (TP) have been synthesized and evaluated for their two photon absorption (2PA) properties. These compounds are virtually non fluorescent in the free state but exhibit a bright red fluorescence upon binding to double-stranded DNA, both in one- and two-photon absorption. This feature allows one- and two-photon confocal imaging in cells of nuclear DNA with an excellent contrast. Derivatizable analogues for covalent bioconjugation to oligonucleotides are described and variation on the structure is discussed.
Bordeau
G
G
Institut Curie, CNRS UMR-176, Centre Universitaire, 91405 Orsay, France.
Faurel
E
E
Allain
C
C
Lartia
R
R
Schmidt
F
F
Fiorini-Debuisschert
C
C
Charra
F
F
Metge
G
G
Tauc
P
P
Teulade-Fichou
M-P
MP
D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal; D017785:Photons; D011726:Pyridinium Compounds / Q000737:chemistry*
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D004247:DNA