Molecularly imprinted polymer of 5-methyluridine for solid-phase extraction of pyrimidine nucleoside cancer markers in urine.
Normal and modified urinary nucleosides represent potential biomarkers for cancer diagnosis. To selectively extract modified nucleosides, we developed a molecularly imprinted polymer (MIP) of 5-methyluridine as selective material for molecularly imprinted solid-phase extraction (MISPE). The MIPs were obtained from vinyl-phenylboronate ester derivative of the template, acrylamide and pentaerythritol triacrylate co-polymer, and were tested in batch and cartridge experiments with aqueous samples. Our results indicated that the imprinted polymer was selective for pyrimidine nucleosides with a K(d) and a B(max) of 46 microM and 18 micromol/g, respectively. Finally, a MISPE of the most common pyrimidine nucleoside cancer markers in urine sample was realized.
Jégourel
Damien
D
Institut de Chimie Organique et Analytique (ICOA), CNRS UMR 6005, Université d'Orléans, BP 6759, Orléans 45067 Cedex 2, France.
Delépée
Raphaël
R
Breton
Florent
F
Rolland
Antoine
A
Vidal
Richard
R
Agrofoglio
Luigi A
LA
D020106:Acrylamide / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D000818:Animals; D015415:Biomarkers / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D013058:Mass Spectrometry / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D054802:Molecular Imprinting / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis / Q000652:urine*; D011108:Polymers / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry; D011741:Pyrimidine Nucleosides / Q000737:chemistry / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry
D000179:Acrylates; D015415:Biomarkers; D011108:Polymers; D011409:Propylene Glycols; D011741:Pyrimidine Nucleosides; D014753:Vinyl Compounds; C009182:ribothymidine; D020106:Acrylamide; C027992:pentaerythrityl triacrylate; D014529:Uridine