[A study of osteogenic induction of dental pulp stem cells from deciduous teeth in vitro].
URPOSE: To isolate and culture dental pulp stem cells from human deciduous teeth, and induce them to osteoblasts.


METHODS
Dental pulp stem cells were separated from deciduous teeth with enzyme digestionìthen isolated and purified by limited dilution. The cells clone form rate was carried out, and cell growth curve was measured by counting the number of cells. HE stain and immunhistochemistry stain of VimentinìCD44 and STRO-1 were tested. Then the cells were induced to osteoblasts, and HE stain,AKP stain, Von Kossa stain,Van Gieson stain,and immunhistochemistry stain of osteocalcin were used for evaluation.


RESULTS
Dental pulp stem cells were obtained from deciduous teeth by limited dilution. The cells were induced to osteoblasts, which elicited a biological and morphologic characteristics similar to those of osteoblasts.


CONCLUSIONS
The results show that dental pulp stem cells are obtained from deciduous teeth and induced to osteoblasts successfully.
Lu
Jia-yu
JY
Department of Stomatology, The Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China. angelinelu2005@hotmail.com
Hua
Li
L
Zhou
Wen-rui
WR
Zou
De-rong
DR
D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003782:Dental Pulp; D006801:Humans; D066298:In Vitro Techniques; D010006:Osteoblasts; D013234:Stem Cells; D014094:Tooth, Deciduous