Identification of oral cancer cell-induced changes in gene expression profile of lymphatic endothelial cell.
Understading functional properties of tumor-derived lymphatic endothelial cells (TLEC) are relevant for blocking lymphatic metastasis. The changes of lymphatic endothelial cells (LEC) cocultured with oral cancer cells in a vitro model were examined. TLEC, in contrast to LEC, were more proliferative and have enhanced ability of lymphangiogenesis and anti-apoptosis. Gene microarrays revealed that 677 unique genes had two-fold or higher change between the two groups. Differential expressions of selected genes were confirmed by real-time PCR. Our results indicate that TLEC display abnormal characteristics and are distinct at the molecular level. Manipulation of TLEC is encouraging for therapy of lymphatic metastasis.
Zhuang
Zhang
Z
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Sichuan, China.
Jian
Pan
P
Longjiang
Li
L
Bo
Han
H
Hongwei
Zhao
Z
D017209:Apoptosis; D002455:Cell Division; D002874:Chromosome Mapping; D002877:Chromosomes, Human / Q000235:genetics; D017931:DNA Primers; D015536:Down-Regulation; D042783:Endothelial Cells / Q000473:pathology*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D017931:DNA Primers; D009363:Neoplasm Proteins