title: Optical filtering systems for wavelength selection in light microscopy.
abstract: Because of its sensitivity and specificity, fluorescence-based detection is one of the foremost methods for microscopic imaging of biological tissues and cells. Dramatic improvements in filter system design and implementation coupled with development of an ever-widening range of sensitive fluorescent dyes have made multicolor imaging a powerful tool for structural and functional analysis. This revised and expanded unit reviews some of the main principles and developments of optical filtering.
authors.affiliation: Carnegie Mellon University, Pittsburgh, Pennsylvania, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Waggoner
authors.forename: E S
authors.initials: ES
authors.lastname: Wachman
authors.forename: D L
authors.initials: DL
authors.lastname: Farkas
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007834:Lasers; D008027:Light; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D055095:Optics and Photonics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes