Expression of TERF1 in rice regulates expression of stress-responsive genes and enhances tolerance to drought and high-salinity.
Drought and high-salinity are the important constraints that severely affect plant development and crop yield worldwide. It has been established that ethylene response factor (ERF) proteins play important regulatory roles in plant response to abiotic and biotic stresses. Our previous researches have revealed that transgenic tobacco over-expressing TERF1 (encoding a tomato ERF protein) showed enhanced tolerance to abiotic stress. Here, we further investigate the function of TERF1 in transgenic rice. Compared with the wild-type plants, overexpression of TERF1 resulted in an increased tolerance to drought and high-salt in transgenic rice. And the enhanced tolerance may be associated with the accumulation of proline and the decrease of water loss. Furthermore, TERF1 can effectively regulate the expression of stress-related functional genes Lip5, Wcor413-l, OsPrx and OsABA2, as well as regulatory genes OsCDPK7, OsCDPK13 and OsCDPK19 under normal growth conditions. Our analyses of cis-acting elements show that there exist DRE/CRT and/or GCC-box existing in TERF1 targeted gene promoters. Our results revealed that ectopic expression of TERF1 in rice caused a series of molecular and physiological alterations and resulted in the transgenic rice with enhanced tolerance to abiotic stress, indicating that TERF1 might have similar regulatory roles in response to abiotic stress in tobacco and rice.
Gao
Shumei
S
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
Zhang
Haiwen
H
Tian
Yun
Y
Li
Fang
F
Zhang
Zhijin
Z
Lu
Xiangyang
X
Chen
Xiaoliang
X
Huang
Rongfeng
R
D001483:Base Sequence; D017931:DNA Primers; D055864:Droughts; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride
D017931:DNA Primers; D010940:Plant Proteins; D012965:Sodium Chloride
Phytochemistry. 2004 Jun;65(12):1745-76
15276435
FEBS Lett. 2004 Aug 27;573(1-3):110-6
15327984
Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16402-9
17923667
Plant Physiol. 2003 May;132(1):64-74
12746512
Plant Cell. 2005 Nov;17(11):3155-75
16214899
Annu Rev Plant Biol. 2003;54:93-107
14502986
Plant Physiol. 2003 Dec;133(4):1755-67
14645724
Nature. 2006 Aug 10;442(7103):705-8
16900200
Biochem Soc Trans. 2005 Apr;33(Pt 2):375-9
15787610
Trends Plant Sci. 2006 Jul;11(7):329-34
16782394
Trends Plant Sci. 2006 Sep;11(9):434-9
16890475
Plant Mol Biol. 2007 Mar;63(5):591-608
17225073
Plant Cell Rep. 2006 Dec;25(12):1263-74
16858552
Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):12987-92
16924117
Trends Plant Sci. 2007 Aug;12(8):343-51
17629540
Plant Cell. 2006 Oct;18(10):2767-81
17041150
Plant J. 2002 Aug;31(3):279-92
12164808
Plant Physiol. 2005 May;138(1):341-51
15834008
Plant Biotechnol J. 2007 Sep;5(5):646-56
17614953
Planta. 2005 Oct;222(3):494-501
15871029
Plant Cell. 2006 Aug;18(8):2021-34
16816135
FEBS Lett. 2007 Mar 20;581(6):1179-89
17336300
Curr Opin Plant Biol. 2007 Aug;10(4):366-71
17644023
J Exp Bot. 2004 Jan;55(395):205-12
14673026
Curr Opin Plant Biol. 2007 Jun;10(3):296-302
17468040
Plant Physiol. 2001 Mar;125(3):1248-57
11244106
Plant Physiol. 2003 Jan;131(1):317-25
12529539
Trends Plant Sci. 2006 Aug;11(8):405-12
16843036
Metab Eng. 2002 Jan;4(1):49-56
11800574
Acta Biochim Pol. 2007;54(2):219-33
17446936
Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15270-5
17881564
Biotechnol Lett. 2007 Jan;29(1):165-73
17136305
Plant Cell Physiol. 2006 Jan;47(1):141-53
16284406
Plant Mol Biol. 2005 Sep;59(1):151-64
16217609
Plant Cell. 2004 Sep;16(9):2481-98
15319476
Plant J. 2003 Feb;33(4):751-63
12609047
J Exp Bot. 2007;58(2):253-65
17132712
Gene. 2007 Feb 15;388(1-2):1-13
17134853
Theor Appl Genet. 2003 Oct;107(6):1071-82
12827255
Trends Plant Sci. 2005 Feb;10(2):88-94
15708346
Plant Physiol. 2000 Apr;122(4):1129-36
10759508
Curr Opin Plant Biol. 2006 Apr;9(2):189-95
16483835
Plant Mol Biol. 2004 Jul;55(4):541-52
15604699
Arch Biochem Biophys. 2005 Dec 15;444(2):139-58
16309626
Plant J. 2000 Aug;23(3):319-27
10929125
Plant J. 1999 Apr;18(2):185-93
10363370
Plant Cell. 1997 Oct;9(10):1859-68
9368419
Annu Rev Plant Biol. 2005;56:165-85
15862093
J Plant Physiol. 2006 Nov;163(11):1167-78
16436304
Plant Cell Physiol. 2001 Nov;42(11):1228-33
11726707
Plant Cell. 2002 Nov;14(11):2837-47
12417705