title: Separation of transition metals on a poly-iminodiacetic acid grafted polymeric resin column with post-column reaction detection utilising a paired emitter-detector diode system.
abstract: The selectivity, retention and separation of transition metals on a short (2 mm x 50 mm) column packed with a poly-iminodiacetic acid functionalised polymer 10 microm resin (Dionex ProPac IMAC-10) are presented. This stationary phase, typically used for the separation of proteins, is composed of long chain poly-iminodiacetic acid groups grafted to a hydrophilic layer surrounding a 10 microm polymeric bead. Through the use of a combination of a multi-step pH and picolinic acid gradient, the separation of magnesium, iron, cobalt, cadmium, zinc, lead and copper was possible, followed by post-column reaction with 4-(2-pyridylazo) resorcinol (PAR) and absorbance detection at 510 nm using a novel and inexpensive optical detector, comprised of two light emitting diodes with one acting as a light source and the other as a detector. Column efficiency for selective transition metals was in excess of N=10,000, with the baseline separation of seven metal cations in <3 min possible under optimised conditions. Detection limits of between 5 and 81 microg/L were possible based upon a 50 microL injection volume.
authors.affiliation: National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland.
authors.forename: Leon
authors.initials: L
authors.lastname: Barron
authors.forename: Martina
authors.initials: M
authors.lastname: O'Toole
authors.forename: Dermot
authors.initials: D
authors.lastname: Diamond
authors.forename: Pavel N
authors.initials: PN
authors.lastname: Nesterenko
authors.forename: Brett
authors.initials: B
authors.lastname: Paull
mesh_terms: D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D007098:Imino Acids / Q000737:chemistry*; D009994:Osmolar Concentration; D011108:Polymers / Q000737:chemistry*; D012118:Resorcinols / Q000737:chemistry; D013696:Temperature; D028561:Transition Elements / Q000032:analysis*
chemical_list: D007098:Imino Acids; D011108:Polymers; D012118:Resorcinols; D028561:Transition Elements; C028398:4-(2-pyridylazo)resorcinol; C008109:iminodiacetic acid