Influence of saliva substitute films on initial Streptococcus mutans adhesion to enamel and dental substrata.
OBJECTIVE
The aim of this in vitro study was to evaluate whether saliva substitutes containing antimicrobial agents influence the initial adhesion of Streptococcus mutans to bovine enamel and various dental materials.


METHODS
Specimens of a denture base resin, a veneering composite and a dental ceramic were prepared according to the manufacturers instructions and polished. Standardized bovine enamel slabs were prepared for reference. Surface roughnesss and surface free energy were determined. Fifteen specimens of each substratum were rinsed with four saliva substitutes (Salinum, Aldiamed, Saliva natura and Saliva Orthana), a negative (PBS) and a positive control (protein mixture) for 2h at 37 degrees C in a flow chamber, and were subsequently exposed to S. mutans NCTC 10449 suspension for 4h at 37 degrees C. Adherent bacteria were quantified using a fluorometric assay. Statistical analysis was performed using one- and two-way ANOVA (p<0.05), and post hocs were analyzed using the Tukey-Kramer multiple comparison test (p<0.05).


RESULTS
Substrata as well as saliva substitutes influenced fluorescence intensities decisively. No significant differences in fluorescence intensities indicating similar adhesion of S. mutans were found between substrata that had been exposed to the negative control, the positive control, Saliva Orthana and Aldiamed. On substrata with high surface free energy (ceramic and bovine enamel), significantly higher fluorescence intensities indicating higher adhesion of streptococci were found to specimens that had been exposed to Saliva natura and Salinum.


CONCLUSIONS
The influence of saliva substitutes on initial S. mutans adhesion appears to be dependent on the substratum surface properties. Only little influence of antimicrobial agents was found.
Hahnel
Sebastian
S
Department of Prosthetic Dentistry, Regensburg University Medical Center, Regensburg D-93042, Germany. sebastian.hahnel@klinik.uni-regensburg.de
Rosentritt
Martin
M
Handel
Gerhard
G
Bürgers
Ralf
R
D000180:Acrylic Resins / Q000737:chemistry; D000504:Aloe / Q000737:chemistry; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002021:Buffers; D002417:Cattle; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical; D003188:Composite Resins / Q000737:chemistry; D003743:Dental Enamel / Q000382:microbiology*; D003764:Dental Materials / Q000737:chemistry*; D003775:Dental Polishing; D003776:Dental Porcelain / Q000737:chemistry; D003801:Dental Veneers; D003819:Denture Bases; D031322:Eriodictyon / Q000737:chemistry; D005470:Fluorometry; D018020:Lithium Compounds / Q000737:chemistry; D008422:Materials Testing; D009077:Mucins / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D012464:Saliva, Artificial / Q000737:chemistry / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects*; D013499:Surface Properties; D013500:Surface Tension; D014993:Xylitol / Q000737:chemistry / Q000494:pharmacology
D000180:Acrylic Resins; D002021:Buffers; D003188:Composite Resins; D003764:Dental Materials; C118557:Empress 2; D018020:Lithium Compounds; D009077:Mucins; C507521:Palapress Vario; D010936:Plant Extracts; C450899:Salinum; D012464:Saliva, Artificial; C477352:Sinfony composite resin; C072342:saliva Orthana; C535155:saliva natura; D003776:Dental Porcelain; D002482:Cellulose; C002283:hydroxyethylcellulose; D014993:Xylitol