title: Complex N-glycans or core 1-derived O-glycans are not required for the expression of stage-specific antigens SSEA-1, SSEA-3, SSEA-4, or Le(Y) in the preimplantation mouse embryo.
abstract: The glycan epitopes termed stage-specific embryonic antigens (SSEA) occur on glycoproteins and glycolipids in mammals. However, it is not known whether these epitopes are attached to N- or O-glycans on glycoproteins and/or on glycolipids in the developing mouse embryo. In this paper the expression of the antigens SSEA-1, SSEA-3, SSEA-4 and Le(Y) was examined on ovulated eggs, early embryos and blastocysts lacking either complex and hybrid N-glycans or core-1 derived O-glycans. In all cases, antigen expression determined by fluorescence microscopy of bound monoclonal antibodies to embryos at the stage of development of maximal expression was similar in mutant and control embryos. Thus, none of these developmental antigens are expressed solely on either complex N- or core 1-derived O-glycans attached to glycoproteins in the preimplantation mouse embryo. Furthermore, neither of these classes of glycan is essential for the expression of SSEA-1, SSEA-3, SSEA-4 or Le(Y) on mouse embryos.
authors.affiliation: Department of Cell Biology, Albert Einstein College of Medicine, New York, NY 10461, USA.
authors.forename: Suzannah A
authors.initials: SA
authors.lastname: Williams
authors.forename: Pamela
authors.initials: P
authors.lastname: Stanley
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D002236:Carbohydrate Conformation; D005260:Female; D007983:Lewis Blood Group Antigens / Q000378:metabolism*; D016256:Lewis X Antigen / Q000378:metabolism; D051379:Mice; D009154:Mutation / Q000235:genetics; D017351:N-Acetylglucosaminyltransferases; D010063:Ovum / Q000166:cytology / Q000201:enzymology; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; C094162:Lewis Y antigen; D011134:Polysaccharides; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3; C066584:stage-specific embryonic antigen-4; D000217:Acyltransferases; C459903:MGAT1 protein, mouse; D017351:N-Acetylglucosaminyltransferases
references.citation: Biochem Biophys Res Commun. 1981 Jun;100(4):1578-86
references.pmid: 6117281
references.citation: FASEB J. 2008 Jul;22(7):2273-84
references.pmid: 18276833
references.citation: Glycoconj J. 2004;21(1-2):41-5
references.pmid: 15467397
references.citation: Genesis. 2000 Feb;26(2):110-2
references.pmid: 10686600
references.citation: Bioessays. 1990 Apr;12(4):173-9
references.pmid: 1970725
references.citation: Adv Exp Med Biol. 1982;152:167-77
references.pmid: 6127917
references.citation: Nature. 1985 Mar 7-13;314(6006):53-7
references.pmid: 2579340
references.citation: J Exp Med. 1984 Nov 1;160(5):1591-6
references.pmid: 6491606
references.citation: J Embryol Exp Morphol. 1985 Dec;90:335-61
references.pmid: 3009679
references.citation: Eur Urol. 1993;23(1):30-6; discussion 36-7
references.pmid: 7682952
references.citation: Cell. 1982 Oct;30(3):697-705
references.pmid: 6183004
references.citation: Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15631-6
references.pmid: 14673092
references.citation: Differentiation. 1988 Jul;38(2):124-33
references.pmid: 3209003
references.citation: Histochem J. 1993 Jun;25(6):452-63
references.pmid: 7689550
references.citation: Dev Biol. 1987 Jun;121(2):568-75
references.pmid: 2884155
references.citation: Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565-9
references.pmid: 281705
references.citation: Cell Differ. 1985 May;16(3):169-73
references.pmid: 2860973
references.citation: Mol Cell Biol. 2004 May;24(10):4221-8
references.pmid: 15121843
references.citation: J Exp Zool. 1987 Dec;244(3):395-408
references.pmid: 3443830
references.citation: Mol Cell Biol. 2004 Nov;24(22):9920-9
references.pmid: 15509794
references.citation: Mol Hum Reprod. 1998 Mar;4(3):295-300
references.pmid: 9570276
references.citation: J Biol Chem. 1983 Jul 25;258(14):8934-42
references.pmid: 6863318
references.citation: Curr Top Dev Biol. 1979;13 Pt 1:139-65
references.pmid: 396115
references.citation: J Biol Chem. 1983 Oct 10;258(19):11793-7
references.pmid: 6194156
references.citation: Genesis. 2005 Dec;43(4):175-80
references.pmid: 16283624
references.citation: Dev Biol. 1984 Aug;104(2):449-60
references.pmid: 6745495
references.citation: J Cell Sci. 2007 Apr 15;120(Pt 8):1341-9
references.pmid: 17374637
references.citation: Development. 2004 Sep;131(18):4435-45
references.pmid: 15306566
references.citation: Glycobiology. 1997 Oct;7(7):913-9
references.pmid: 9363433
references.citation: J Cell Biochem. 1988 Jan;36(1):1-14
references.pmid: 3277983
references.citation: Biol Reprod. 1995 Apr;52(4):903-12
references.pmid: 7780013
references.citation: Biochem J. 1983 Dec 1;215(3):491-503
references.pmid: 6197967
references.citation: Dev Biol. 1984 May;103(1):117-28
references.pmid: 6370756
references.citation: EMBO J. 1983;2(12):2355-61
references.pmid: 6141938
references.citation: Nature. 1981 Jul 9;292(5819):156-8
references.pmid: 6165896
references.citation: Histochem J. 1993 Sep;25(9):628-41
references.pmid: 7693623