title: Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of Aspergillus niger.
abstract: Chitosan and its derivatives have been used as affinity matrices for purification of lipase from Aspergillus niger NCIM 1207. Trimellitic anhydride (TMA)-crosslinked deacetylated chitin adsorbed lipase selectively, yielding approximately 5-fold purification of the crude lipase with 70% yield. Further 9-fold purification occurred on eluting through Sephacryl-100. These results suggest that chitosan derivatives can be used as inexpensive biopolymer matrices for the purification of lipases for industrial applications.
authors.affiliation: Polymer Science and Engineering Division, National Chemical Laboratory, Pune 411008, India.
authors.forename: K D
authors.initials: KD
authors.lastname: Trimukhe
authors.forename: N D
authors.initials: ND
authors.lastname: Mahadik
authors.forename: D V
authors.initials: DV
authors.lastname: Gokhale
authors.forename: A J
authors.initials: AJ
authors.lastname: Varma
mesh_terms: D000107:Acetylation; D000327:Adsorption; D001234:Aspergillus niger / Q000201:enzymology*; D048271:Chitosan / Q000031:analogs & derivatives; D003432:Cross-Linking Reagents / Q000493:pharmacokinetics; D004777:Environment; D008049:Lipase / Q000302:isolation & purification* / Q000493:pharmacokinetics*
chemical_list: D003432:Cross-Linking Reagents; D048271:Chitosan; D008049:Lipase