The protective effects of taurine against early renal injury in STZ-induced diabetic rats, correlated with inhibition of renal LOX-1-mediated ICAM-1 expression.
Oxidative stress is a key cause in the development of diabetic nephropathy (DN). As a main receptor of oxidized low-density lipoprotein (oxLDL), LOX-1 plays an important role in the induction of leukocyte adhesion molecules, such as intercellular cell adhesion molecule-1 (ICAM-1). Taurine (TAU), a potent endogenous antioxidant, showed renoprotective effects in several model animals. This study was designed to determine the renoprotective effect and possible mechanism involved LOX-1 and ICAM-1 expression of taurine in early DN. Six-week-old male Wistar rats were divided into three groups: normal control (NC), diabetes mellitus (DM), and taurine-treated DM (DM+TAU). Diabetes was induced by streptozotocin (STZ, 60 mg/kg, i.p.). After the onset of diabetes, drinking water containing 1% taurine was given to rats in the DM+TAU group. After six weeks of treatment, blood glucose (BG), serum levels of creatinine (sCr) and BUN, and LOX-1 and ICAM-1 expression (protein and gene) in kidney cortices were estimated. Meanwhile, renal malondialdehyde (MDA) levels and glutathione peroxidase (GSH-Px) activities were examined as parameters of oxidative stress in diabetic rats. For DM+TAU rats, when compared with DM rats, the levels of serum BUN, sCr, and renal MDA were reduced, and the activities of renal GSH-Px were increased, but the BG levels were not influenced. Simultaneously, taurine attenuated histopathologic evidence of renal damages and reduced the overexpression of LOX-1 and ICAM-1 in kidney cortices of diabetic rats. In conclusion, taurine showed protective effects against early renal injury in diabetic rats. These renoprotective effects may be partly caused by suppression of oxLDL/LOX-1 system and subsequently ICAM-1 overexpression on renal cortex via its antioxidative property.
Wang
Lijun
L
Department of Paediatrics, Provincial Hospital Affiliated to ShanDong University, Jinan, China.
Zhang
Ligong
L
Yu
Yonghui
Y
Wang
Yi
Y
Niu
Na
N
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000517:prevention & control*; D003928:Diabetic Nephropathies / Q000139:chemically induced / Q000517:prevention & control*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D051127:Scavenger Receptors, Class E / Q000187:drug effects*; D013311:Streptozocin; D013654:Taurine / Q000494:pharmacology* / Q000627:therapeutic use*
C495136:OLR1 protein, rat; D051127:Scavenger Receptors, Class E; D018799:Intercellular Adhesion Molecule-1; D013654:Taurine; D013311:Streptozocin