Dietary supplementation with N-acetylcysteine, alpha-tocopherol and alpha-lipoic acid prevents age related decline in Na(+),K (+)-ATPase activity and associated peroxidative damage in rat brain synaptosomes.
This study has shown that in aged rat brain (22-24 months) crude synaptosomes in comparison to that in young animals (4-6 months), a striking decrease in the activity of Na(+),K(+)-ATPase occurs along with decreased K (m) and V (max) but without any change in enzyme content as seen by immunoblotting. This is associated with an accumulation of peroxidative damage products in aged brain. When rats are given antioxidant supplementation in the diet with a combination of N-acetylcysteine, alpha-tocopherol and alpha-lipoic acid daily from 18 months onwards and sacrificed at 22-24 months for experimentation, the age associated decrease of Na(+),K(+)-ATPase activity, alterations of its kinetic parameters and accumulation of peroxidative damage products in brain synaptosomes are prevented nearly completely. Because of the critical importance of Na(+),K(+)-ATPase in neuronal functions, the results of this study may be of potential implications in controlling age-related functional deficits of the brain.
Bagh
Maria Bindu
MB
Department of Biochemistry, Institute of Postgraduate Medical Education and Research (Dr. B.C. Roy Post Graduate Institute of Basic Medical Sciences), 244B, Acharya J.C. Bose Road, Kolkata, 700020, India.
Maiti
Arpan Kumar
AK
Roy
Arun
A
Chakrabarti
Sasanka
S
D000111:Acetylcysteine / Q000494:pharmacology*; D000375:Aging / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D019587:Dietary Supplements; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013574:Synaptosomes / Q000187:drug effects / Q000201:enzymology*; D008063:Thioctic Acid / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology*
D000975:Antioxidants; D016166:Free Radical Scavengers; D008063:Thioctic Acid; D000254:Sodium-Potassium-Exchanging ATPase; D024502:alpha-Tocopherol; D000111:Acetylcysteine