title: Synthesis of a naphthalene diimide derivative having four ferrocene moieties as an electrochemical DNA hybridization indicator.
abstract: A new naphthalene diimide derivative carrying four ferrocene moieties, F(4)ND, was synthesized by the reaction of trimethylammoniummethylferrocene with the terminal amino moieties of two imide substituents of naphthalene diimide. Spectrophotometric binding studies of F(4)ND with calf thymus DNA showed its high affinity of 2 x 10(5) M(-1) in 10 mM MES buffer and 1 mM EDTA (pH 6.25) containing 0.10 M NaCl. The Osteryoung square wave voltammetry (SWV) of a DNA probe-immobilized electrode before and after hybridization with target DNA gave a larger peak current in an electrolyte containing F(4)ND than that containing FND carrying two ferrocene moieties. The current peak difference between mismatched and fully matched DNA was also greater with F(4)ND than that with FND.
authors.affiliation: Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu 804-8550, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sato
authors.forename: Masato
authors.initials: M
authors.lastname: Tsueda
authors.forename: Sadayoshi
authors.initials: S
authors.lastname: Watanabe
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ohtsuka
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry; D015342:DNA Probes; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D007094:Imides / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D005296:Ferrous Compounds; D007094:Imides; D004247:DNA; C427203:calf thymus DNA