Evolution of arsenate toxicity in nodulated white lupine in a long-term culture.
White lupine is an As-resistant legume that is of interest for phytoremediation of As-contaminated soils. To achieve successful phytoremediation, monitoring of the nutritional status of the selected plant species during the entire culture cycle is required to maintain a plant cover with high biomass production. A long-term pot experiment was carried out with nodulated lupine grown on perlite with 10 and 100 microM As concentrations. The reproductive period (from 10 weeks) was the most sensitive phenologic stage of white lupine to long-term As exposure. The 10 microM As treatment increased the uptake and translocation of micronutrients, except for Cu, mainly at flowering with As levels in pods below the statutory limit (1 mg kg (-1) fresh weight). However, the 100 microM As treatment induced significant differences compared to the control. These findings confirm the relatively high resistance of white lupine to arsenate and support the use of this species in phytoremediation and/or revegetation of As-contaminated sites, with special attention on P and Cu nutrition at flowering.
Vázquez
Saúl
S
Department of Agricultural Chemistry, Facultad de Ciencias, Universidad Autónoma de Madrid, Carretera Colmenar Km 15, 28049 Madrid, Spain. saul.vazquez@uam.es
Esteban
Elvira
E
Carpena
Ramón O
RO
D001149:Arsenates / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*; D003300:Copper / Q000378:metabolism; D031297:Lupinus / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D018977:Micronutrients / Q000032:analysis / Q000378:metabolism; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism
D001149:Arsenates; D018977:Micronutrients; D010758:Phosphorus; D003300:Copper; C025657:arsenic acid