High-sensitivity molecular recognition with light-induced polymerization.
A novel strategy for enhanced molecular recognition that utilized standard complexation strategies in combination with light-induced, or photo-, polymerization was recently demonstrated. Creative and rational materials design aided in the development of macroinitiators that produce a specific binding event, which is then amplified through photo-initation and chain polymerization. This polymerization-based amplification system produced a positive result that was visibly recognizable with amounts as low as 1000 molecules.
Guymon
C Allan
CA
Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA 52246, USA. allan-guymon@uiowa.edu
D001360:Avidin / Q000737:chemistry / Q000528:radiation effects; D001709:Biotechnology / Q000379:methods*; D001710:Biotin / Q000737:chemistry / Q000528:radiation effects; D025202:Molecular Diagnostic Techniques; D011108:Polymers / Q000737:chemistry / Q000528:radiation effects*; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
D011108:Polymers; D001360:Avidin; D001710:Biotin