Strategies for accessing soil metagenome for desired applications.
Most of the microorganisms in nature are inaccessible as they are uncultivable in the laboratory. Metagenomic approaches promise the accessibility of the genetic resources and their potential applications. Genetic resources from terrestrial environments can be accessed by exploring the soil metagenome. Soil metagenomic analyses are usually initiated by the isolation of environmental DNAs. Several methods have been described for the direct isolation of environmental DNAs from soil and sediments. Application of metagenomics largely depends on the construction of genomic DNA libraries and subsequent high-throughput sequencing or library screening. Thus, obtaining large quantities of pure cloneable DNA from the environment is a prerequisite. This review discusses the recent developments related to efficient extraction and purification of soil metagenome highlighting the considerations for various metagenomic applications.
Rajendhran
J
J
Department of Genetics, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai-625 021, India.
Gunasekaran
P
P
D001435:Bacteriophages / Q000235:genetics; D044822:Biodiversity; D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000302:isolation & purification*; D016680:Genome, Bacterial; D016679:Genome, Viral; D015698:Genomic Library; D023281:Genomics; D006812:Humic Substances / Q000032:analysis; D010957:Plasmids; D012988:Soil Microbiology
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D006812:Humic Substances