title: [N-ras and fms gene mutation in idiopathic thrombocytopenic purpura and myelodysplasia].
abstract: OBJECTIVE
  To explore the pathogenesis of idiopathic thrombocytopenic purpura (ITP) and improve the differential diagnosis from myelodysplastic syndromes (MDS).
  
  
  METHODS
  Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was performed to detect the point mutation of codon 12,13 in N-ras gene and codon 301, 969 in fms gene in adult and aged ITP and MDS patients.
  
  
  RESULTS
  In 25 ITP patients, N-ras mutation and fms mutation were detected in one each (4%). Mutations were found in 3 of 8 MDS patients: two (25%) with N-ras mutation and one (12.5%) with fms mutation.
  
  
  CONCLUSIONS
  Patients with N-ras or fms gene mutation diagnosed as MDS rather than ITP.
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Hou
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Li
authors.forename: Dao-Xin
authors.initials: DX
authors.lastname: Ma
authors.forename: Qi-Ji
authors.initials: QJ
authors.lastname: Liu
authors.forename: Pin
authors.initials: P
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D016236:Genes, fms / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics*