Identification of weakly beta-hemolytic porcine spirochetes by biochemical reactions, PCR-based restriction fragment length polymorphism analysis and species-specific PCR.
We examined the usefulness of PCR-based restriction fragment length polymorphism (PCR-RFLP) and species-specific PCR combined with a newly devised rapid biochemical test using microplates for identifying weakly beta-hemolytic intestinal spirochetes (WBHIS) isolated from pigs. WBHIS strains showing atypical biochemical characteristics were decisively identified at the species level by PCR-RFLP and species-specific PCR. Identification of WBHIS at the species level in routine diagnostic work will certainly contribute to clarifying the pathogenicity of WBHIS.
Ohya
Tatsuo
T
Kyushu Research Station, Environmental/Enzootic Diseases Research Team, National Institute of Animal Health, Chuzan, Kagoshima, Japan. ohyat@affrc.go.jp
Araki
Hiroshi
H
Sueyoshi
Masuo
M
D000818:Animals; D017400:Brachyspira / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology