Simple and rapid colorimetric detection of low-weight molecules using aptazymes in combination with noncrosslinking gold nanoparticle aggregation.
We developed a method for simply and rapidly detecting cofactors of aptazymes with high sensitivity using a unique noncrosslinking gold nanoparticle aggregation. Applying this method to a theophylline dependent aptazyme, 100 microM, 10 microM, and 1 microM theophylline were detected easily by the naked eye within 10 min, 20 min, and 65 min, respectively. This method is applicable to other cleavage-aptazymes without altering the probe-DNA sequence.
Ogawa
Atsushi
A
Bioengineering Laboratory, RIKEN-The Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan. a-ogawa@riken.jp
Maeda
Mizuo
M
D003124:Colorimetry / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry*; D013806:Theophylline / Q000032:analysis
D015342:DNA Probes; D016337:RNA, Catalytic; D012313:RNA; D006046:Gold; D013806:Theophylline