The AtrbohD-mediated oxidative burst elicited by oligogalacturonides in Arabidopsis is dispensable for the activation of defense responses effective against Botrytis cinerea.
Oligogalacturonides (OGs) are endogenous elicitors of defense responses released after partial degradation of pectin in the plant cell wall. We have previously shown that, in Arabidopsis (Arabidopsis thaliana), OGs induce the expression of PHYTOALEXIN DEFICIENT3 (PAD3) and increase resistance to the necrotrophic fungal pathogen Botrytis cinerea independently of signaling pathways mediated by jasmonate, salicylic acid, and ethylene. Here, we illustrate that the rapid induction of the expression of a variety of genes by OGs is also independent of salicylic acid, ethylene, and jasmonate. OGs elicit a robust extracellular oxidative burst that is generated by the NADPH oxidase AtrbohD. This burst is not required for the expression of OG-responsive genes or for OG-induced resistance to B. cinerea, whereas callose accumulation requires a functional AtrbohD. OG-induced resistance to B. cinerea is also unaffected in powdery mildew resistant4, despite the fact that callose accumulation was almost abolished in this mutant. These results indicate that the OG-induced oxidative burst is not required for the activation of defense responses effective against B. cinerea, leaving open the question of the role of reactive oxygen species in elicitor-mediated defense.
Galletti
Roberta
R
Dipartimento di Biologia Vegetale, Università di Roma La Sapienza, 5-00185 Rome, Italy.
Denoux
Carine
C
Gambetta
Stefano
S
Dewdney
Julia
J
Ausubel
Frederick M
FM
De Lorenzo
Giulia
G
Ferrari
Simone
S
D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism / Q000382:microbiology*; D020171:Botrytis / Q000472:pathogenicity*; D003517:Cyclopentanes / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism*; D024101:Mitochondrial Proteins; D010088:Oxidoreductases / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism; D010940:Plant Proteins; D016897:Respiratory Burst; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction; D000099188:Alternative Oxidase
D003517:Cyclopentanes; D005030:Ethylenes; D006603:Hexuronic Acids; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D054883:Oxylipins; D010940:Plant Proteins; D020156:Salicylic Acid; D000099188:Alternative Oxidase; C007819:galacturonic acid; C011006:jasmonic acid; C036216:ethylene
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D575-7
14681484
Plant Cell. 1997 Sep;9(9):1559-1572
12237396
New Phytol. 2001 Jul;151(1):185-194
33873377
Plant Cell Rep. 2008 Jan;27(1):125-35
17786451
Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W504-9
16845059
Proc Natl Acad Sci U S A. 1999 May 25;96(11):6553-7
10339626
Plant Cell. 2000 Nov;12(11):2175-90
11090217
Plant Physiol. 2004 Jan;134(1):460-9
14730073
Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):517-22
11756663
FEBS Lett. 1990 Jul 30;268(1):69-71
2384174
Plant Cell. 1998 Mar;10(3):359-70
9501110
Mol Plant Microbe Interact. 2003 Dec;16(12):1118-28
14651345
J Exp Bot. 2002 May;53(372):1255-72
11997374
Plant Physiol. 1993 May;102(1):233-240
12231814
Mol Plant Microbe Interact. 2003 Mar;16(3):179-87
12650449
Planta. 2004 Mar;218(5):767-74
14618326
Plant Physiol. 2007 Apr;143(4):1817-26
17277088
Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):8955-9
8090752
Plant J. 2005 Oct;44(1):25-36
16167893
Plant Cell. 1990 Jun;2(6):513-23
2152173
Science. 2007 Feb 23;315(5815):1098-103
17185563
Plant Physiol. 1987 Aug;84(4):1286-90
16665599
Plant Physiol. 1989 Jun;90(2):542-8
16666805
Mol Plant Microbe Interact. 2006 Aug;19(8):931-6
16903359
Mol Plant Microbe Interact. 2001 Jun;14(6):725-36
11386368
Mol Plant. 2008 May;1(3):423-45
19825551
Phytochemistry. 1998 May;48(2):223-7
9637062
Science. 1998 May 15;280(5366):1091-4
9582125
Curr Opin Plant Biol. 2002 Aug;5(4):318-24
12179965
J Exp Bot. 2002 May;53(372):1367-76
11997382
Cell. 1997 Jan 10;88(1):57-63
9019406
Plant Physiol. 1981 Nov;68(5):1161-9
16662068
Plant Cell. 2003 Jan;15(1):93-106
12509524
Plant J. 1998 Sep;15(6):747-54
9807813
C R Acad Sci III. 1993 Aug;316(8):793-8
8044704
Cell. 1994 Nov 18;79(4):583-93
7954825
Plant Physiol. 2006 Aug;141(4):1248-54
16766671
Cell Microbiol. 2007 Jun;9(6):1385-96
17451411
Plant Physiol. 2005 Oct;139(2):847-56
16183833
Plant Physiol. 1986 Feb;80(2):568-77
16664663
Plant Physiol. 2006 Jun;141(2):373-8
16760490
Plant Mol Biol. 2004 Aug;55(6):797-805
15604717
Free Radic Res. 1995 Dec;23(6):517-32
8574346
Plant Cell. 1996 Mar;8(3):477-487
12239391
Plant J. 2007 Sep;51(5):931-40
17651370
Plant J. 2004 Apr;38(1):119-30
15053765
Immunol Rev. 2004 Apr;198:249-66
15199967
Plant Cell. 2003 Mar;15(3):706-18
12615943
Nat Genet. 2005 Oct;37(10):1130-4
16170317
J Biol Chem. 2003 Jan 24;278(4):2256-64
12426314
Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6837-40
11607311
Trends Plant Sci. 2003 Jun;8(6):245-7
12818655
Plant J. 1999 May;18(3):265-76
10377992
Plant Physiol. 2004 Sep;136(1):2621-32
15375207
Plant J. 2003 Jul;35(2):193-205
12848825
Plant Cell. 1991 Feb;3(2):127-36
1840904
Nucleic Acids Res. 2001 May 1;29(9):e45
11328886
Plant Cell Physiol. 2005 Jun;46(6):947-54
15821288
Nature. 2001 Nov 29;414(6863):562-5
11734859
Plant J. 1999 May;18(3):277-84
10377993
Plant Physiol. 1989 May;90(1):109-16
16666719
Curr Opin Plant Biol. 2005 Aug;8(4):397-403
15939662
Curr Biol. 2000 Jun 29;10(13):751-7
10898976
Plant Physiol. 2000 Apr;122(4):1379-85
10759534
Plant Cell. 2006 May;18(5):1310-26
16603654
Planta. 2002 Aug;215(4):596-605
12172842
Plant J. 2005 Mar;41(5):673-84
15703055
Plant Physiol. 2006 Apr;140(4):1222-32
16428598
Plant Cell. 1996 Oct;8(10):1809-1819
12239363
Plant Physiol Biochem. 2004 May;42(5):429-35
15191747
J Exp Bot. 2006;57(10):2435-44
16766599
Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):9969-73
1946465
Plant Physiol. 1999 Sep;121(1):163-72
10482671
Mol Plant Microbe Interact. 2001 Apr;14(4):439-50
11310731
Cell Res. 2004 Jun;14(3):234-40
15225417
Plant Physiol. 2007 May;144(1):367-79
17384165
Science. 2003 Aug 15;301(5635):969-72
12920300
Cell Host Microbe. 2007 May 17;1(3):175-85
18005697
Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:601-639
15012221
J Exp Bot. 2006;57(6):1373-9
16551687
Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:251-275
15012264