title: Association of cytokine gene polymorphisms with bronchial asthma in Macedonians.
abstract: Bronchial asthma is a multifactorial disease whereby both environmental and genetic factors contribute to its aetiology and/or clinical severity. The aim of this study was to examine the association of 22 cytokine gene polymorphism in the Macedonian population with bronchial asthma (BA). The sample of the population comprised of 301 normal unrelated individuals and 74 patients with BA. Cytokine genotyping was performed by PCR. Susceptible cytokine polymorphisms for BA for ten genotypes (IL-4 -1098/T:T, TNF-alpha -238/A:G, IL-4 -590/C:C, IL-2 +166/T:T, IL-2 -330/T:T, IL-10 -1082/G:G, IFNgamma utr5644/T:T, IL-10 -1082/A:A, IL-1beta +3962/T:T, IL-6 -174/G:G), six diplotypes, four haplotypes, and two alleles were found. Protective cytokine polymorphisms for BA for seven cytokine genotypes (IL-4 -1098/G:T, TNF- alpha -238/G:G, IL-2 -330/G:T, IL-4 -590/C:T, IFNgamma utr5644/A:T, IL-1beta +3962/C:T, IL-10 -1082/A:G), six cytokine diplotypes, four cytokine haplotypes, and four cytokine alleles were found. We concluded that several cytokine polymorphisms are protective, or susceptible associated with BA in population of Macedonians.
authors.affiliation: Institute of Immunobiology and Human Genetics, Faculty of Medicine, University "St. Kiril and Metodij", Skopje, Republic of Macedonia.
authors.forename: Dejan
authors.initials: D
authors.lastname: Trajkov
authors.forename: Jagoda
authors.initials: J
authors.lastname: Mirkovska-Stojkovikj
authors.forename: Todor
authors.initials: T
authors.lastname: Arsov
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Petlichkovski
authors.forename: Ana
authors.initials: A
authors.lastname: Strezova
authors.forename: Olivija
authors.initials: O
authors.lastname: Efinska-Mladenovska
authors.forename: Emilija
authors.initials: E
authors.lastname: Sandevska
authors.forename: Jean
authors.initials: J
authors.lastname: Gogusev
authors.forename: Mirko
authors.initials: M
authors.lastname: Spiroski
mesh_terms: D000328:Adult; D001249:Asthma / Q000235:genetics* / Q000276:immunology; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D005260:Female; D005787:Gene Frequency / Q000276:immunology; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D018843:Republic of North Macedonia
chemical_list: D016207:Cytokines; D007376:Interleukin-2