title: Liquid chromatography-mass spectrometry method for the determination of thiamphenicol in rabbit tears.
abstract: A simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was described for the determination of thiamphenicol in rabbit tears. Chromatographic separation of the analyte was achieved on a C18 column using a mobile phase of acetonitrile and 10 mmol/l ammonium acetate solution (60:40, v/v). Selected ion monitoring (SIM) in negative mode was used for analyte quantification at m/z 354.4 for thiamphenicol and at m/z 137.1 for salylic acid. The run time was less than 6 min. Linearity over the concentration range of 0.032-32.0 ng/ml for thiamphenicol was obtained and the lower limit of quantification was 0.032 ng/ml. For each level of QC samples, inter- and intra-day precisions (R.S.D.) were <or=5.2% and 8.3%, respectively, and the accuracy (RE) was +/-2.8%. The present LC-MS method was successfully applied to the pharmacokinetic studies of thiamphenicol in situ forming gel in rabbit tears.
authors.affiliation: Department of Pharmacy, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
authors.forename: Dafeng
authors.initials: D
authors.lastname: Tian
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Jianhong
authors.initials: J
authors.lastname: Yang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Tian
authors.forename: Fengkui
authors.initials: F
authors.lastname: Mo
mesh_terms: D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D002138:Calibration; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004355:Drug Stability; D005615:Freezing; D005782:Gels / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D011786:Quality Control; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D013666:Tears / Q000737:chemistry*; D013696:Temperature; D013839:Thiamphenicol / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D013997:Time Factors
chemical_list: D000085:Acetates; D000097:Acetonitriles; D000900:Anti-Bacterial Agents; D005782:Gels; D012996:Solutions; D013839:Thiamphenicol; C018824:ammonium acetate; C032159:acetonitrile