[Immunolocalization of receptor activator of NF-kappaB ligand and osteoprotegerin during mouse mandibular first molar eruption].
OBJECTIVE
To investigate the immunolocalization of receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG), and to explore the correlation between their expressions and activity of osteoclast during first mandibular molar eruption.


METHODS
Mouse mandibles dissected from postnatal day 1.5 to 14.5 were stained respectively for multinucleated osteoclasts using tartrate-resistant acid phosphatase (TRAP) staining, and RANKL and OPG protein expression was examined by immunohistochemical staining.


RESULTS
The two peak values of osteoclast/acreage in the occlusal and basal region were both observed on the P1.5 and P9.5; while the two peak values in the lateral region were on P3.5 and P9.5. During the mouse molar eruption, burst of osteoclastogenesis was associated with high expression of RANKL and low expression of OPG.


CONCLUSIONS
RANKL and OPG could have a close relationship with the osteoclast activity and two developmental apexes were observed during the molar eruption. The occlusal movement was relatively stable, meanwhile the temporarily accelerative movement to the basal and lateral regions could occur.
Wu
Ling
L
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan, China.
Hua
Fang
F
Zhang
Xiao-lei
XL
Zhang
Lu
L
Chen
Zhi
Z
D000818:Animals; D051379:Mice; D008807:Mice, Inbred BALB C; D053244:Osteoprotegerin / Q000276:immunology / Q000378:metabolism*; D053245:RANK Ligand / Q000276:immunology / Q000378:metabolism*; D014078:Tooth Eruption / Q000276:immunology
D053244:Osteoprotegerin; D053245:RANK Ligand