Quantitative mass spectrometry as a tool for nutritional proteomics.
The goal of nutritional science is to determine the effect of dietary components. Proteomics has emerged as a method to study proteins on a large scale. The proteomic information gathered from a sample, whether a biological fluid, cell, or tissue, includes not only the identities of proteins present but also their quantities and posttranslational modifications. Recent advances in mass spectrometry make it possible to accurately measure concentrations of thousands of proteins derived from such complex biological samples. Quantitative data will provide increased understanding of the dynamic changes in the proteome induced by dietary components.
Moresco
James J
JJ
Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Dong
Meng-Qiu
MQ
Yates
John R
JR
D002853:Chromatography, Liquid; D004044:Dietary Proteins / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009587:Nitrogen Isotopes / Q000032:analysis; D015596:Nutrition Assessment; D020543:Proteome; D018401:Sample Size
D004044:Dietary Proteins; D009587:Nitrogen Isotopes; D020543:Proteome