Spectrofluorimetric determination of vitamin B(1) using horseradish peroxidase as catalyst in the presence of hydrogen peroxide.
In this work a new spectrofluorimetric method for the determination of vitamin B(1), based on the catalytic activity of horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H2O2), has been developed. Non-fluorescent vitamin B(1) was easily converted through catalytic oxidation in alkaline medium into a fluorescent compound, even without exposure to light. The linear range for vitamin B(1) observed was 0.026-16.83 microg/mL (RSD = 1.75%). The correlation coefficient for the calibration curve and limit of detection were found to be 0.9964 and 0.015 microg/mL, respectively. The developed method is practical, simple, sensitive and relatively free from interference by coexisting substances and has been successfully applied for the determination of vitamin B(1) in pharmaceutical preparations.
Khan
Mir Azam
MA
Department of Chemistry, Kyungpook National University, Taegu 702-701, South Korea.
Jin
Seung Oh
SO
Lee
Sang Hak
SH
Chung
Hye Young
HY
D002138:Calibration; D002384:Catalysis; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013831:Thiamine / Q000032:analysis*
D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D013831:Thiamine