[Primary evaluation of anti-HEV diagnostic reagent by experimental infection animal model with hepatitis E virus].
OBJECTIVE
To evaluate anti-HEV diagnostic kits by experimental infecting rhesus monkeys with HEV.


METHODS
Eight rhesus monkeys were infected with genotype 1 and 4 HEV separately. The alanine aminotransferase (ALT) level of all monkeys were detected before and after the process of infection. HEV RNA in stool specimens was tested by reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Anti-HEV IgG in serum was detected by GL-IgG and WT-IgG.


RESULTS
HEV RNA presented in the stool of all the 8 monkeys after infection. The ALT level of 1 monkey infected with genotype 1 HEV and 2 monkeys infected with genotype 4 HEV appeared abnormally after infection. Tested by GL-IgG, 2 of the 4 monkeys infected with genotype 1 HEV and 1 of 4 monkeys infected with genotype 4 HEV seroconverted to anti-HEV IgG. However, when tested by WT-IgG, all the infected monkeys seroconverted to anti-HEV IgG. The anti-HEV IgG tested by WT-IgG was positive during the whole observation period,and the anti-HEV IgG measured by GL-IgG only remained 12 weeks after infection. Detected by GL-IgG and WT-IgG, seropositive conversion of the anti-HEV IgG happened almost at the same time.


CONCLUSIONS
Both GL-IgG and WT-IgG could detect the anti-HEV IgG of experimentally infected rhesus monkeys but the WT-IgG had a higher sensitivity for detection of anti-HEV IgG than
Zhou
Cheng
C
National Institute for the Control of Pharmaceutical and Biological Product, Beijing 100050, China.
Huang
Wei-jin
WJ
Wu
Xing
X
Lan
Hai-yun
HY
Gu
Wen-jie
WJ
Huang
Guo-yong
GY
Zhang
Hua-yuan
HY
Qi
Zi-bai
ZB
Li
He-min
HM
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D005838:Genotype; D016751:Hepatitis E / Q000276:immunology* / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D008253:Macaca mulatta; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007074:Immunoglobulin G; D000410:Alanine Transaminase