Functionalization of DNA G-Wires for patterning and nanofabrication.
DNA structures made of guanine tetrads present remarkable properties and are thus first choice candidates for applications in nanofabrication. Starting from the work of Kotlyar et al., we report here that the klenow exo(-) fragment of DNA polymerase I can extend poly(dG)-poly(dC) from various 5'-modified (dG)(10(-))(dC)(10) templates. This allows the production of end-functionalized four-stranded wires (G-Wires) assembled from the folding of poly(dG) strands. G-Wires bearing thiol moieties can be easily combed on Au and Pt surfaces, whereas a 5' single-stranded overhang of a random sequence provides the unique possibility to assemble complex structures for nanoconstruction purposes.
Lyonnais
Sébastien
S
Muséum national d'Histoire naturelle USM 503, INSERM U565, CNRS UMR 5153, 43 rue Cuvier 75005 Paris, France. slyonnais@mnhn.fr
Piétrement
Olivier
O
Chepelianski
Alexei
A
Guéron
Sophie
S
Lacroix
Laurent
L
Le Cam
Eric
E
Mergny
Jean-Louis
JL
D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D004256:DNA Polymerase I / Q000378:metabolism; D054856:G-Quadruplexes; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D011068:Poly G / Q000737:chemistry; D011089:Polydeoxyribonucleotides / Q000096:biosynthesis
D011089:Polydeoxyribonucleotides; D011068:Poly G; C016681:poly(dG).poly(dC); C026628:poly(dG); D004247:DNA; D004256:DNA Polymerase I