title: In vivo murine model of continuous intramedullary infusion of particles--a preliminary study.
abstract: Continued production of wear debris affects both initial osseointegration and subsequent bone remodeling of total joint replacements (TJRs). However, continuous delivery of clinically relevant particles using a viable, cost effective, quantitative animal model to simulate the scenario in humans has been a challenge for orthopedic researchers. In this study, we successfully infused blue-dyed polystyrene particles, similar in size to wear debris in humans, to the intramedullary space of the mouse femur for 4 weeks using an osmotic pump. Approximately 40% of the original particle load (85 microL) was delivered into the intramedullary space, an estimate of 3 x 10(9) particles. The visible blue dye carried by the particles confirmed the delivery. This model demonstrated that continuous infusion of particles to the murine bone-implant interface is possible. In vivo biological processes associated using wear debris particles can be studied using this new animal model.
authors.affiliation: The Department of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.forename: Steven G
authors.initials: SG
authors.lastname: Ortiz
authors.forename: Zhinong
authors.initials: Z
authors.lastname: Huang
authors.forename: Peigen
authors.initials: P
authors.lastname: Ren
authors.forename: R Lane
authors.initials: RL
authors.lastname: Smith
authors.forename: Stuart B
authors.initials: SB
authors.lastname: Goodman
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D016723:Bone Remodeling; D018786:Bone Substitutes / Q000737:chemistry*; D004396:Coloring Agents / Q000494:pharmacology; D016503:Drug Delivery Systems; D005269:Femur / Q000473:pathology*; D008297:Male; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D009995:Osmosis; D016348:Osseointegration; D010316:Particle Size; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D004396:Coloring Agents; D014867:Water
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