title: Morphometric and histological analysis of low-power laser influence on bone morphogenetic protein in bone defects repair.
abstract: Bone morphogenetic proteins (BMPs) are secreted signaling molecules belonging to the transforming growth factor-beta (TGF-beta) superfamily. The objective of this study was to determine how gallium-aluminum-arsenium (GaAlAs) 650 nm laser influenced the action of BMPs on bone defects created in rat femurs. The sample consisted of 24 male albino Wistar rats. Group 1 was composed of rats with bone defects filled with bone-inducing substance, with the application of low-power laser. Group 2 contained rats with bone defects filled with a bone-inducing substance, without the application of low-power laser. Group 3 rats had bone defects not filled with a bone-inducing substance, with the application of low-power laser. Group 4 rats had bone defects and no treatment (control group). A bone defect was produced with drills. In groups 1 and 2 the defects were filled with a bone-inducing substance. The animals were treated with GaAlAs (50 mW) laser, energy density 4 J/cm(2), for 80 ss on a 1 cm(2) area. Groups 2 and 4 were used as control. Bone samples were removed for histological procedures and morphometric analysis on the 7th, 14th and 21st days after surgery. Results obtained were subjected to statistical analysis. Rejection level for the null hypothesis was 0.05. Statistical differences were found in the comparison between group 1 (G1), G2, G3 and G4 [analysis of variance (ANOVA); P < 0.0134]. There was a statistically significant correlation between groups 1 and 4 (P < 0.01). The results of other correlations by Tukey's post-hoc test were: group 1 vs group 3 (P = 0.341), group 1 vs group 2 (P = 0.862), group 2 vs group 4 (P = 0.061), group 2 vs group 3 (P = 0.744), and group 3 vs group 4 (P = 0.249). We concluded that the association of low-power laser with a bone-inducing substance produced better results than when low-power laser or BMPs were used alone.
authors.affiliation: Postgraduate Program on Health and Development in West Central Region, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
authors.forename: Amanda Silveira
authors.initials: AS
authors.lastname: Denadai
authors.forename: Paulo de Tarso Camillo
authors.initials: Pde T
authors.lastname: de Carvalho
authors.forename: Filipe Abdalla
authors.initials: FA
authors.lastname: dos Reis
authors.forename: Ana Carulina Guimarães
authors.initials: AC
authors.lastname: Belchior
authors.forename: Daniel Martins
authors.initials: DM
authors.lastname: Pereira
authors.forename: Doroty Mesquita
authors.initials: DM
authors.lastname: Dourado
authors.forename: Iandara S
authors.initials: IS
authors.lastname: Silva
authors.forename: Luis Vicente Franco
authors.initials: LV
authors.lastname: de Oliveira
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology / Q000502:physiology*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D018786:Bone Substitutes; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology / Q000502:physiology / Q000528:radiation effects*; D028022:Low-Level Light Therapy; D008297:Male; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes
references.citation: Calcif Tissue Int. 1999 Jul;65(1):83-9
references.pmid: 10369739
references.citation: J Bone Miner Res. 1999 Nov;14(11):1805-15
references.pmid: 10571679
references.citation: Vet Surg. 1995 Sep-Oct;24(5):408-19
references.pmid: 8585149
references.citation: J Bone Miner Metab. 2006;24(2):95-9
references.pmid: 16502114
references.citation: J Clin Laser Med Surg. 2002 Apr;20(2):83-7
references.pmid: 12017432
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004 Jun;97(6):693-700
references.pmid: 15184850
references.citation: Dev Growth Differ. 2007 Aug;49(6):455-68
references.pmid: 17661740
references.citation: Lasers Surg Med. 2007 Dec;39(10):788-96
references.pmid: 18081142
references.citation: J Clin Laser Med Surg. 1997;15(4):181-3
references.pmid: 9612167
references.citation: Lasers Surg Med. 1996;19(4):458-64
references.pmid: 8983007
references.citation: J Appl Oral Sci. 2004 Mar;12(1):70-7
references.pmid: 21365155