Cardiac isoenzymes in healthy Holstein calves and calves with experimentally induced endotoxemia.
This paper describes a controlled study designed to establish normal values for cardiac troponins I and T (cTnI and cTnT) and CK-MB mass in healthy newborn Holstein calves, and to compare values for cTnI, cTnT, CK-MB and total creatine kinase (CK) with age-matched calves experiencing experimentally induced endotoxemia. Nineteen healthy Holstein bull calves, 48 to 72 h of age were used. Baseline cTnI, cTnT, CK-MB and total CK measurements were obtained from control (n = 9) and experimental (n = 10) calves. Controls then received physiological saline and experimental calves received endotoxin (O55:B5 Escherichia coli LPS) intravenously after which cardiac biomarkers and total CK were measured at 3 h, 6 h, 12 h, and 24 h post-initiation of infusion. Measured values were analyzed and compared using analysis of variance (ANOVA) by repeated measure design, with statistical significance set at P < 0.05. The cardiac biomarker cTnT was not detected in any calf at any time point, and CK-MB was only detected in 5 of 95 samples. The cTnI was significantly increased compared to baseline and controls, 3 h post lipopolysaccharide (LPS) infusion. Total CK was significantly increased in LPS administered calves at 18 and 24 h post infusion. The mean, standard deviation, and range for cTnI in healthy controls were 0.023 ng/mL (s = 0.01), and 0.01 to 0.05 ng/mL, respectively. In conclusion, LPS administration was associated with rapid and significant increases in cTnI but CK-MB and cTnT were not detected in the plasma of healthy calves. Total CK values increased significantly following LPS administration. Biochemical evidence of myocardial injury occurs within 3 h following LPS administration to neonatal Holstein calves.
Peek
Simon F
SF
Large Animal Internal Medicine, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin 53706, USA. peeks@svm.vetmed.wisc.edu
Apple
Fred S
FS
Murakami
Mary Ann
MA
Crump
Peter M
PM
Semrad
Susan D
SD
D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000201:enzymology; D003402:Creatine Kinase / Q000097:blood*; D052279:Creatine Kinase, MB Form / Q000097:blood; D019446:Endotoxemia / Q000097:blood / Q000201:enzymology / Q000662:veterinary*; D007527:Isoenzymes / Q000097:blood; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009203:Myocardial Infarction / Q000097:blood / Q000662:veterinary; D011897:Random Allocation; D012016:Reference Values; D019210:Troponin I / Q000097:blood*; D020107:Troponin T / Q000097:blood*
D015415:Biomarkers; D007527:Isoenzymes; D008070:Lipopolysaccharides; D019210:Troponin I; D020107:Troponin T; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form
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