Fluorogenic probe triggered by reduction for nucleic acids sensing.
A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10-20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.
Furukawa
Kazuhiro
K
Nano Medical Engineering Laboratory, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 Japan.
Abe
Hiroshi
H
Wang
Jin
J
Oki
Kazuma
K
Uda
Miwako
M
Tsuneda
Satoshi
S
Ito
Yoshihiro
Y
D015342:DNA Probes / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D009841:Oligonucleotides / Q000032:analysis*; D010084:Oxidation-Reduction; D012338:RNA, Ribosomal, 23S / Q000032:analysis; D012235:Rhodamines / Q000737:chemistry*
D015342:DNA Probes; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012338:RNA, Ribosomal, 23S; D012235:Rhodamines