title: Chip is required for posteclosion behavior in Drosophila.
abstract: Neurons acquire their molecular, neurochemical, and connectional features during development as a result of complex regulatory mechanisms. Here, we show that a ubiquitous, multifunctional protein cofactor, Chip, plays a critical role in a set of neurons in Drosophila that control the well described posteclosion behavior. Newly eclosed flies normally expand their wings and display tanning and hardening of their cuticle. Using multiple approaches to interfere with Chip function, we find that these processes do not occur without normal activity of this protein. Furthermore, we identified the nature of the deficit to be an absence of Bursicon in the hemolymph of newly eclosed flies, whereas the responsivity to Bursicon in these flies remains normal. Chip interacts with transcription factors of the LIM-HD (LIM-homeodomain) family, and we identified one member, dIslet, as a potential partner of Chip in this process. Our findings provide the first evidence of transcriptional mechanisms involved in the development of the neuronal circuit that regulates posteclosion behavior in Drosophila.
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India.
authors.forename: Padmanabhan
authors.initials: P
authors.lastname: Hari
authors.forename: Mugdha
authors.initials: M
authors.lastname: Deshpande
authors.forename: Neha
authors.initials: N
authors.lastname: Sharma
authors.forename: Neha
authors.initials: N
authors.lastname: Rajadhyaksha
authors.forename: Nitya
authors.initials: N
authors.lastname: Ramkumar
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kimura
authors.forename: Veronica
authors.initials: V
authors.lastname: Rodrigues
authors.forename: Shubha
authors.initials: S
authors.lastname: Tole
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D002116:Calcitonin / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006458:Hemolymph / Q000378:metabolism; D007447:Invertebrate Hormones / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D010446:Peptide Fragments / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D013997:Time Factors; D014921:Wings, Animal / Q000254:growth & development* / Q000378:metabolism*
chemical_list: C108740:Chi protein, Drosophila; D029721:Drosophila Proteins; D007447:Invertebrate Hormones; D009687:Nuclear Proteins; D010446:Peptide Fragments; D034741:RNA, Small Interfering; D015124:8-Bromo Cyclic Adenosine Monophosphate; C037553:katacalcin; D002116:Calcitonin; C002295:bursicon
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