Temporal progression of hypothalamic patterning by a dual action of BMP.
In the developing chick hypothalamus, Shh and BMPs are expressed in a spatially overlapping, but temporally consecutive, manner. Here, we demonstrate how the temporal integration of Shh and BMP signalling leads to the late acquisition of Pax7 expression in hypothalamic progenitor cells. Our studies reveal a requirement for a dual action of BMPs: first, the inhibition of GliA function through Gli3 upregulation; and second, activation of a Smad5-dependent BMP pathway. Previous studies have shown a requirement for spatial antagonism of Shh and BMPs in early CNS patterning; here, we propose that neural pattern elaboration can be achieved through a versatile temporal antagonism between Shh and BMPs.
Ohyama
Kyoji
K
MRC Centre for Developmental and Biomedical Genetics and Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK.
Das
Raman
R
Placzek
Marysia
M
D000818:Animals; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D007031:Hypothalamus / Q000196:embryology* / Q000378:metabolism; D017403:In Situ Hybridization; D008954:Models, Biological; D009924:Organ Culture Techniques; D051763:PAX7 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D051902:Smad5 Protein / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013997:Time Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D019485:Bone Morphogenetic Proteins; D053823:Hedgehog Proteins; D051763:PAX7 Transcription Factor; D051902:Smad5 Protein; D015534:Trans-Activators