title: Titanium acts on osteoblast translational process.
abstract: Titanium is a highly biocompatible material and very osteogenic in vivo. However, how titanium regulates osteoblast activity to promote bone formation is incompletely characterized. We, therefore, attempted to get more information by using microRNA (miRNA) microarray techniques to investigate translation regulation in osteoblasts grown on titanium disks. The miRNA oligonucleotide microarray provides a novel method to carry out genome-wide miRNA profiling in human samples. By using miRNA microarrays containing 329 probes designed from the human miRNA sequence, several miRNA were identified in osteoblast-like cell line (MG 63) grown on titanium disks. There were 13 upregulated miRNAs (ie, mir-23a, mir-222, mir-523, mir-22, mir-23b, mir-143, mir-377, mir-24, mir-422b, mir-26a, mir-29a, mir-17-5p, mir-182) and 2 down-regulated miRNAs (ie, mir-187, mir-339). The data reported are, to our knowledge, the first study on translation regulation in osteoblasts exposed to titanium. The data can be relevant to understand better the molecular mechanism of osteoblast activation and as a model for comparing other materials with similar clinical effects.
authors.affiliation: Institute of Histology, University of Bologna and Center of Molecular Genetics, Cassa di Risparmio di Bologna Foundation, Bologna, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Palmieri
authors.forename: Furio
authors.initials: F
authors.lastname: Pezzetti
authors.forename: Anna
authors.initials: A
authors.lastname: Avantaggiato
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Lo Muzio
authors.forename: Antonio
authors.initials: A
authors.lastname: Scarano
authors.forename: Corrado
authors.initials: C
authors.lastname: Rubini
authors.forename: Laura
authors.initials: L
authors.lastname: Guerzoni
authors.forename: Marzia
authors.initials: M
authors.lastname: Arlotti
authors.forename: Dario
authors.initials: D
authors.lastname: Ventorre
authors.forename: Francesco
authors.initials: F
authors.lastname: Carinci
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D002460:Cell Line; D003764:Dental Materials / Q000494:pharmacology*; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000187:drug effects*; D014176:Protein Biosynthesis / Q000187:drug effects*; D015347:RNA Probes; D014025:Titanium / Q000494:pharmacology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D001672:Biocompatible Materials; D003764:Dental Materials; D015347:RNA Probes; D014025:Titanium