title: Idaten is a new cold-inducible transposon of Volvox carteri that can be used for tagging developmentally important genes.
abstract: A cold-inducible transposon called Jordan has previously been used to tag and recover genes controlling key aspects of Volvox development, including the process called inversion. In a search for additional genes, we isolated 17 new inversionless mutants from cultures grown at 24 degrees (the temperature that activates Jordan transposition). These mutants were stable at 32 degrees, but generated revertants at 24 degrees . DNA blots revealed that one mutant had a transposon unrelated to Jordan inserted in invA ("inversionless A"). This new transposon, which we named Idaten, has terminal inverted repeats (TIRs) beginning with CCCTA, and upon insertion it creates a 3-bp target-site duplication. It appears to belong to the CACTA superfamily of class II DNA transposons, which includes En/Spm. No significant open reading frames were in the Idaten sequence, but we retrieved another element with Idaten-type TIRs encoding a protein similar to the En/Spm transposase as a candidate for an Idaten-specific transposase. We found that in five of the new inversionless strains we could not find any Jordan insertions causing the phenotype to possess insertions of an Idaten family member in a single locus (invC). This clearly indicates that Idaten is a potentially powerful alternative to Jordan for tagging developmentally important genes in Volvox.
authors.affiliation: RIKEN Advanced Science Institute, Wako-shi, Saitama, 351-0198, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Ueki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nishii
mesh_terms: D020418:Algal Proteins / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003080:Cold Temperature; D004251:DNA Transposable Elements / Q000235:genetics*; D048028:DNA, Algal / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018745:Genome, Plant; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction; D018626:Retroelements / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D020079:Terminal Repeat Sequences / Q000235:genetics; D019895:Transposases; D044446:Volvox / Q000502:physiology*
chemical_list: D020418:Algal Proteins; D004251:DNA Transposable Elements; D048028:DNA, Algal; D018744:DNA, Plant; D018626:Retroelements; D019895:Transposases
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