Interaction of USF1/USF2 and alpha-Pal/Nrf1 to Fmr-1 promoter increases in mouse brain during aging.
Fragile X syndrome is caused due to silencing of FMR-1 gene transcription leading to loss of fragile X mental retardation protein (FMRP). To investigate whether the transcriptional mechanism is linked to aging, we have studied interaction of the transcription factors USF1/USF2 and alpha-Pal/Nrf1 to E-box and GC-box, respectively, in Fmr-1 promoter in the brain of young, adult, and old mouse using electrophoretic mobility shift assay (EMSA). Our data reveal that the interaction of these transcription factors to their respective promoter sequences increases in mouse brain as a function of age. The finding on the interaction of the above transcription factors to their cognate sequences is novel as the current investigation has been carried out in intact and aging mouse. The present finding is important in respect to age- and FMRP-dependent brain function.
Prasad
S
S
Biochemistry & Molecular Biology Lab, CAS in Zoology, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India. drsprasad_bhu@yahoo.co.uk
Singh
Kanchan
K
D000367:Age Factors; D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D024721:E-Box Elements; D024202:Electrophoretic Mobility Shift Assay; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics*; D020862:GC Rich Sequence; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D051266:Nuclear Respiratory Factor 1 / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D051720:Upstream Stimulatory Factors / Q000378:metabolism*
D051860:Fragile X Messenger Ribonucleoprotein 1; D051266:Nuclear Respiratory Factor 1; D051720:Upstream Stimulatory Factors; C497684:Fmr1 protein, mouse; C495634:Nrf1 protein, mouse; C497183:Usf1 protein, mouse; C497186:Usf2 protein, mouse