Characterization of the phenylalanine ammonia-lyase gene (SlPAL5) from tomato (Solanum lycopersicum L.).
Phylogenetic analysis based on the deduced amino acid sequence of phenylalanine ammonia-lyase gene (SlPAL5) cDNA from tomato (Solanum lycopersicum L.) revealed high sequence similarity to PAL genes in Nicotiana tabacum (92%), Ipomoea nil (87%), Manihot esculenta (84%), and Catharanthus roseus (84%). The SlPAL5 gene exists as multiple copies in the tomato plant, and its transcription was strongly expressed in old leaves and flowers. From 5 days post-anthesis to the onset of ripening, SlPAL5 expression decreased gradually but was maintained at a comparatively high level; SlPAL5 transcript expression was very low at the mature-green stage. SlPAL5 expression was significantly induced in response to NaCl, mannitol, and cold treatment; SlPAL5 expression decreased gradually after treatment with abscisic acid and H(2)O(2); SlPAL5 transcript decreased after exposure to methyl viologen for 3 h and increased after 6 h and maintained a stable expression level until 24 h, suggesting that the SlPAL5 gene may function in the response to abiotic stress.
Guo
Jia
J
School of Biotechnology, Kangwon National University, Chuncheon, Kangwon-do, 200-701, South Korea.
Wang
Myeong-Hyeon
MH
D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology; D017343:Genes, Plant / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics* / Q000502:physiology; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D018514:Plant Structures / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics
D010650:Phenylalanine Ammonia-Lyase
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