Synthesis of 3'-deoxyapionucleoside triphosphates and their incorporation into DNA by DNA polymerase.
Here, we report the synthesis of 3'-deoxyapionucleoside 3''-triphosphates (apioNTPs) and the analysis of their property as substrate of enzymatic polymerization. We established the large scale synthetic route of 3-deoxy-D-apiose from D-galactose, and regio- and stereo-selective glycosylation procedures. Resulting 3'-deoxyapionucleosides were then converted into their 3''-triphosphates. We carried out primer-extension reactions and found that Therminator polymerase, a variant of 9 degrees N DNA polymerase, was an efficient enzyme for incorporation of apioNTPs to synthesize DNA-dependent 3'-deoxy-D-apiose nucleic acids (apioNAs).
Kataoka
Mayumi
M
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Sato
Kousuke
K
Matsuda
Akira
A
D004247:DNA / Q000096:biosynthesis / Q000737:chemistry*; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D010429:Pentoses / Q000737:chemistry*
D017931:DNA Primers; D003854:Deoxyribonucleotides; D010429:Pentoses; D004247:DNA; C004983:apiose; D004259:DNA-Directed DNA Polymerase