Quantitative flow cytometric analysis of membrane antigen expression.
Immunological analysis for cell antigens has been performed by flow cytometry in a qualitative fashion for over thirty years. During that time it has become increasingly apparent that quantitative measurements such as number of antigens per cell provide unique and useful information. This unit on quantitative flow cytometry (QFCM) describes the most commonly used protocols, both direct and indirect, and the major methods of analysis for the number of antibody binding sites on a cell or particle. Practical applications include detection of antigen under- or overexpression in hematological malignancies, distinguishing between B cell lymphoproliferative disorders, and precise diagnosis of certain rare diseases.
D'hautcourt
Jean-Luc
JL
Centre Hospitalier Régional Mons, Warquignies, Boussu, Belgium.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism*; D002138:Calibration; D002462:Cell Membrane / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D007202:Indicators and Reagents; D007962:Leukocytes / Q000378:metabolism; D051379:Mice; D008863:Microspheres; D010799:Phycoerythrin / Q000378:metabolism; D012044:Regression Analysis; D017186:Titrimetry
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007202:Indicators and Reagents; D010799:Phycoerythrin