title: CD4+CD25+ regulatory T cells are infected and activated during acute FIV infection.
abstract: HIV-induced AIDS may be mediated by the activation of immunosuppressive CD4+CD25+ T regulatory cells (Treg cells). Treg cells have been shown to regulate CD4+ and CD8+ immune responses to HIV and FIV antigens in vitro. We tested the hypothesis that Treg cells become infected and activated during the acute infection with FIV leading to the suppression of CD4+ T helper cell responses. Cats were experimentally infected with FIV-NCSU1 and blood and lymph node cells were collected at weekly intervals following inoculation. Real-time RT-PCR was used to determine plasma viremia and the relative expression of FIV, FoxP3, TGF-beta, and GAPDH mRNA copies in CD4+CD25+ and CD4+CD25- T cell subsets. Flow cytometry was used to assess the absolute numbers of each cell type and the expression of surface TGF-beta and intracellular FoxP3 in CD4+CD25+ and CD4+CD25- T cells at each time-point. Treg suppression of IL-2 production in CD4+ T helper cells was assessed by ELISPOT assays. Our results showed that peak viremia occurred at 2 weeks post infection and correlated with maximal infectivity in CD4+CD25+ T cell populations. FIV-gag-mRNA levels were higher in CD4+CD25+ T cells than CD4+CD25- T cells throughout the acute phase of infection. Induction of FoxP3 and TGF-beta indicated activation of Treg cells during the acute stage infection, which was confirmed by Treg cell suppression of IL-2 production by CD4+ Th cells in an ELISPOT assay. Our findings support the hypothesis that early activation of Treg immunosuppressor function may limit an effective anti-FIV response, contributing to the establishment of chronic infection and the immunodeficiency caused by this virus.
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, United States.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Mexas
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Fogle
authors.forename: Wayne A
authors.initials: WA
authors.lastname: Tompkins
authors.forename: Mary B
authors.initials: MB
authors.lastname: Tompkins
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology*; D005434:Flow Cytometry / Q000662:veterinary; D051858:Forkhead Transcription Factors / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology*; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D050378:T-Lymphocytes, Regulatory / Q000821:virology*; D014766:Viremia / Q000662:veterinary
chemical_list: D051858:Forkhead Transcription Factors; D007376:Interleukin-2
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