Changes of neuron-specific and apoptosis gene expression levels after ventromedial hypothalamic lesions in rat intestine.
The intestinal epithelium is continuously renewed through a balance between cell proliferation and apoptosis. We identified genes of which expression profiles showed significant modulation, and we investigated the cellular mechanisms of this gene regulation in rat intestine after ventromedial hypothalamic (VMH) lesions. Total RNA was extracted, and differences in the gene expression profiles between rats at day 3 after VMH lesioning and in sham-VMH lesioned rats were investigated using DNA microarray analysis and real-time polymerase chain reaction (PCR) methods. DNA microarray analysis revealed that VMH lesions regulated the genes that were involved in functions predominantly related to neuronal development, cell proliferation and apoptosis. Real-time PCR also confirmed that gene expressions of Efnb1 were downregulated. Meanwhile, expression of Casp3 was similar. It is noted that the signaling networks of many gene families, including neuron-specific genes and apoptosis genes in the intestine were changed after VMH lesioning. VMH lesions may suppress mainly the caspase independent type II pathway for apoptosis and induce cell proliferation in the intestine.
Kiba
Takayoshi
T
Laboratory of Clinical Nutrition & Physiology, Kyoritsu Women's University, 2-2-1 Hitotsubashi, Chiyoda-ku, Tokyo, 101-8433, Japan. takkiba@hotmail.com
Kintaka
Yuri
Y
Suzuki
Yoko
Y
Nakata
Eiko
E
Osaka
Toshimasa
T
Ishigaki
Yasuhito
Y
Inoue
Shuji
S
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014697:Ventromedial Hypothalamic Nucleus / Q000378:metabolism / Q000473:pathology*