Functional markers for selection of potyvirus resistance alleles at the pvr2-eIF4E locus in pepper using tetra-primer ARMS-PCR.
Functional markers targeted on single nucleotide polymorphisms (SNPs) responsible for phenotypic variation constitute optimal tools for marker-assisted selection (MAS) of resistance alleles with different specificities. Here, we used the tetra-primer ARMS-PCR procedure to assay SNP signatures of four distinct alleles at the pvr2-eIF4E locus, which controls pepper resistance to several potyviruses. These simple, economical, and codominant markers open the way for MAS of pepper genotypes resistant to the potyviral strains and species that are prevalent in distinct cultivation areas across the world.
Rubio
Manuel
M
INRA, Centre d'Avignon, UR1052, Unité de Génétique et Amélioration des Fruits et Légumes, BP 94, 84143 Montfavet CEDEX, France.
Caranta
Carole
C
Palloix
Alain
A
D000483:Alleles; D001483:Base Sequence; D002212:Capsicum / Q000235:genetics* / Q000821:virology; D017931:DNA Primers; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics*; D005819:Genetic Markers; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D017800:Potyvirus / Q000472:pathogenicity*
D017931:DNA Primers; D039561:Eukaryotic Initiation Factor-4E; D005819:Genetic Markers