[Study on determination of acetamiprid and interaction between acetamiprid and deoxyribonucleic acid by resonance light scattering].
The interaction between acetamiprid and deoxyribonucleic acid (DNA) was used to determine acetamiprid by resonance light scattering (RLS). The RLS signals of DNA were greatly enhanced by acetamiprid in the spectrum region of 300-600 nm. The spectrum peak is around 316.0 nm. The optimum conditions: pH is 1.73; the concentration of DNA is 2.0 microg x mL(-1)bration curve is 0-2. 25 pg * mLU , with the detection2limit of 0. 2 ig * mL '. The acetamiprid in river water sample was determined. The results were satisfactory, and the recovery rates were in the range of 98%-106%. The interaction mechanism of acetamiprid and DNA was discussed: the interactions between acetamiprid and nucleic acid base include electrostatic effect and Tr-r cumulate effect.
Du
Feng-pei
FP
College of Science, China Agricultural University, Beijing 100094, China. dufp@cau.edu.cn
Liu
Gang
G
Luo
Xiao-lin
XL
Hou
Shi-cong
SC
Li
Ke-an
KA
Ren
Li-ping
LP
Huang
Qin
Q
Mao
Zhao-shu
ZS
Jie
Nian-qin
NQ
D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008027:Light; D000073943:Neonicotinoids; D011725:Pyridines / Q000032:analysis* / Q000737:chemistry; D012542:Scattering, Radiation; D055672:Static Electricity
D000073943:Neonicotinoids; D011725:Pyridines; C464485:acetamiprid; D004247:DNA