title: Voluntary exercise alters GABA(A) receptor subunit and glutamic acid decarboxylase-67 gene expression in the rat forebrain.
abstract: Voluntary exercise improves stress coping and lowers anxiety. Because of the role of GABA in these processes, we investigated changes in the central GABAergic system in rats with free access to a running wheel for 4 weeks. The control animals had no access to a running wheel. Using insitu hybridisation histochemistry, we studied changes in gene expression of various GABA(A) receptor subunits as well as the GABA-synthesising enzyme glutamic acid decarboxylase-67 (GAD67) in the forebrain. There were region-specific decreases in alpha2, beta3 and gamma2 subunit mRNA expression and region-specific increases in beta1 subunit expression. The alpha5 and delta subunits, in the forebrain specifically associated with extrasynaptic GABA(A) receptors in the hippocampus, showed differential increases in expression levels. Expression of GAD67 mRNA was increased in many forebrain regions including all hippocampal cell layers, peri-paraventricular nucleus, bed nucleus stria terminalis, nucleus accumbens core and motor cortex, suggesting that long-term voluntary exercise enhances forebrain GABA synthesis capacity but in a region-specific manner. Thus, regular performance of exercise results in extensive changes in the forebrain GABAergic system that may be implicated in the changes in stress sensitivity and emotionality observed in exercising subjects.
authors.affiliation: Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Bristol, UK.
authors.forename: L E
authors.initials: LE
authors.lastname: Hill
authors.forename: S K
authors.initials: SK
authors.lastname: Droste
authors.forename: D J
authors.initials: DJ
authors.lastname: Nutt
authors.forename: A C E
authors.initials: AC
authors.lastname: Linthorst
authors.forename: J M H M
authors.initials: JM
authors.lastname: Reul
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D005786:Gene Expression Regulation; D005968:Glutamate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D009043:Motor Activity; D009928:Organ Specificity; D005082:Physical Exertion; D016548:Prosencephalon / Q000166:cytology / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000235:genetics* / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1