title: Induction of lymphoid leukosis transplant able tumours and the establishment of lymphoblastoid cell lines.
abstract: Primary lymphoid tumours induced by the subgroup A, B, C and D lymphoid leukosis viruses RAV-1, RAV-2, RAV-49 and RAV-50, respectively, have been developed as transplantable "B-cell" lymphoid tumours and designated LSCT-RP5, LSCT-RP6, LSCT-RP7 and LSCT-RP8, respectively. There seems little doubt that these are indeed transplants because all produce tumours and death of the inoculated chicks in a very short time (median days to death =5-10 days), and chromosome analyses reveal that the sex of the tumour cells remains unchanged when passaged in chicks of the opposite sex. We have established 2 in vitro lymphoblastoid lines (LSCC-RP9 and LSCC-RP12) from the RAV-2 transplantable tumour (LSCT-RP6), but were unsuccessful after numerous attempts with RAV-1 transplantable tumours (LSCT-RP5). The LSCC-RP9 and LSCC-RP12 lymphoblastoid cell lines grow rapidly and produce large amounts of virus. These transplantable tumours and lymphoblastoid cell lines should provide valuable materials for studies on virus-host and tumour cell-host interrelationships.
authors.affiliation: U.S. Department of Agriculture, Science and Education Administration, Agricultural Research, Regional Poultry Research Laboratory, East Lansing, Michigan 48823, USA.
authors.forename: W
authors.initials: W
authors.lastname: Okazaki
authors.forename: R L
authors.initials: RL
authors.lastname: Witter
authors.forename: C
authors.initials: C
authors.lastname: Romero
authors.forename: K
authors.initials: K
authors.lastname: Nazerian
authors.forename: J M
authors.initials: JM
authors.lastname: Sharma
authors.forename: A
authors.initials: A
authors.lastname: Fadly
authors.forename: D
authors.initials: D
authors.lastname: Ewert