Transcriptomic analysis of the exit from dormancy of Aspergillus fumigatus conidia.
BACKGROUND
Establishment of aspergillosis is depending upon the exit from dormancy and germination of the conidia of Aspergillus fumigatus in the lung. To gain an understanding of the molecular mechanisms underlying the early steps of conidial germination, we undertook a transcriptomic analysis using macroarrays constructed with PCR fragments from > 3,000 genes (around one third of the annotated A. fumigatus genome).


RESULTS
Major results of this analysis are the following: (i) conidia stored pre-packaged mRNAs transcripts (27% of genes have transcripts in the resting conidia; (ii) incubation at 37 degrees C in a nutritive medium induced up- and down-regulation of genes: 19% of the total number of genes deposited on the array were up-regulated whereas 22% of the genes with pre-packaged mRNA in the resting conidia were down-regulated; (iii) most modifications were seen during the first 30 min of germination whereas very little modification of gene expression occurred during the following hour; (iv) one-year old conidia and one-week old conidia behaved similarly at transcriptional level.


CONCLUSIONS
Transcriptomic data indicate that the exit from dormancy is associated with a shift from a fermentative metabolism to a respiratory metabolism as well as a trend toward immediate protein synthesis.
Lamarre
Claude
C
Unité des Aspergillus, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France. mechant_boris@hotmail.com
Sokol
Sergueï
S
Debeaupuis
Jean-Paul
JP
Henry
Christine
C
Lacroix
Céline
C
Glaser
Philippe
P
Coppée
Jean-Yves
JY
François
Jean-Marie
JM
Latgé
Jean-Paul
JP
D001232:Aspergillus fumigatus / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger
Genes Chromosomes Cancer. 2006 Jan;45(1):1-10
16175572
Mol Genet Genomics. 2005 Jun;273(5):394-403
15887033
Cell. 1980 Oct;21(3):709-15
6449291
Med Mycol. 2001 Apr;39(2):195-8
11346268
Fungal Genet Biol. 2008 Mar;45(3):232-42
17703972
Biochemistry. 2006 Feb 14;45(6):1979-86
16460045
Eukaryot Cell. 2006 Nov;5(11):1934-40
16998074
Fungal Genet Biol. 2004 Feb;41(2):199-212
14732266
Diagn Microbiol Infect Dis. 2006 Aug;55(4):279-91
16626916
Fungal Genet Biol. 2005 Jul;42(7):638-45
15896988
Infect Immun. 2004 Sep;72(9):5193-203
15322014
J Biol Chem. 2007 Apr 13;282(15):11078-83
17303556
Mol Biol Cell. 2004 Dec;15(12):5295-305
15456898
Fungal Genet Biol. 2004 Mar;41(3):317-26
14761792
Cell. 2003 Oct 17;115(2):135-8
14567911
Eukaryot Cell. 2004 Apr;3(2):557-60
15075285
BMC Evol Biol. 2006 Dec 19;6:109
17178000
Appl Environ Microbiol. 2006 Feb;72(2):1667-71
16461724
Dev Biol. 1980 Aug;78(2):497-510
6997115
Nucleic Acids Res. 2005 Nov 14;33(20):6469-85
16287898
Eukaryot Cell. 2002 Feb;1(1):33-43
12455969
Microbiology (Reading). 1999 Oct;145 ( Pt 10):2625-34
10537184
BMC Genomics. 2007 Apr 11;8:100
17428342
Genetics. 2000 Jun;155(2):647-56
10835388
Microbiol Mol Biol Rev. 2004 Jun;68(2):187-206
15187181
Nature. 2005 Dec 22;438(7071):1151-6
16372009
Curr Genet. 2006 Jul;50(1):32-44
16622700
Annu Rev Physiol. 2007;69:145-70
17037980
J Biol Chem. 2002 Oct 25;277(43):40505-12
12181322
Fungal Genet Biol. 2004 Apr;41(4):391-400
14998522
Mol Biol Cell. 2005 Dec;16(12):5866-79
16207816
FEMS Microbiol Lett. 2001 May 30;199(2):153-60
11377860
FEMS Yeast Res. 2005 Dec;5(12):1253-9
16099222
Mol Microbiol. 2003 Mar;47(6):1613-25
12622816
Antimicrob Agents Chemother. 2002 Mar;46(3):615-24
11850239
Mol Biol Cell. 1994 Oct;5(10):1065-80
7865875
Mol Microbiol. 1999 May;32(3):471-83
10320571
Microbiol Mol Biol Rev. 1998 Mar;62(1):35-54
9529886
Fungal Genet Biol. 2002 Nov;37(2):197-204
12409104
Trends Plant Sci. 2007 May;12(5):217-23
17416545
Eukaryot Cell. 2004 Oct;3(5):1101-10
15470238
Mol Cell Biol. 2002 Oct;22(20):6993-7003
12242280
Appl Microbiol Biotechnol. 1998 Aug;50(2):193-8
9763690
Biochemistry. 2005 Jan 25;44(3):893-904
15654745
Plant Physiol. 2001 Jan;125(1):180-8
11154327
Nucleic Acids Res. 1996 Apr 15;24(8):1428-34
8628674
Mol Biol Cell. 1998 Oct;9(10):2963-71
9763455
Infect Immun. 2006 Aug;74(8):4865-74
16861675
J Biol. 2007;6(2):4
17439666
PLoS Pathog. 2007 Apr;3(4):e50
17432932