title: Densitometric quantification of ether-type phospholipids.
abstract: A quantification method for analysis of individual ether-type phospholipids is important in studies of the regulation of membrane lipid biosynthesis in Archaea. For ester-type lipid of Bacteria and Eucarya, a densitometric method has been established for simultaneous quantification of individual phospholipids visualized with molybdenum blue reagent on a TLC plate. In this study, we developed a TLC densitometric method for rapid quantitative determination of 6 kinds of main ether-type phospholipids in a methanogenic archaeon and an extremely halophilic archaeon. It has been reported previously that on densitometric quantification the values of molar absorptivities are approximately the same among most ester-type phospholipids. On the other hand, we found significant disparity in the molar absorptivity of archaeal ether-type lipids and serine-containing ester-type lipid. Therefore, analysis should be accomplished by use of each standard mixture. Compared with a previous method (preparative TLC method) that is measurement of inorganic phosphate of silica gel powder scraped off from spots of phospholipids on a TLC plate, the TLC densitometry is accomplished at one tenth the sample size in a short time.
authors.affiliation: Department of Chemistry, School of Medicine, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Oe
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Mami
authors.initials: M
authors.lastname: Ohga
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Koga
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morii
mesh_terms: D001105:Archaea / Q000737:chemistry; D002855:Chromatography, Thin Layer; D003720:Densitometry; D010742:Phospholipid Ethers / Q000032:analysis*
chemical_list: D010742:Phospholipid Ethers