Synthesis of locked S-type and locked N-type uridine monomer units for incorporation in 2'-5' RNA:3'-5'RNA duplexes.
To delineate the structural requirements of 2'-5' RNA:3'-5' RNA duplexes, we report the synthesis of N-type locked, S-type locked and 3'-fluoro-2'-phosphoramidite building blocks. The consequent incorporation of these three novel monomers into 2'-5' linked oligomers and their biophysical implications on the stability of the said duplexes will have explicit importance towards development into therapeutic oligomers. The intrinsic stability of 2'-5' phosphodiester linkage as opposed to 3'-5' linked oligomers will be an added advantage.
Gunjal
Anita D
AD
Division of Organic Chemistry, National Chemical Laboratory, Pune, 411008 India.
Erande
Namrata
N
D'Costa
Moneesha
M
Kumar
Vaijayanti A
VA
D004247:DNA / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D012330:RNA, Double-Stranded / Q000737:chemistry*; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D012330:RNA, Double-Stranded; C477371:locked nucleic acid; C434331:phosphoramidite; D004247:DNA; D014529:Uridine