Interaction of bovine adenovirus-3 33K protein with other viral proteins.
The adenovirus 33K protein appears to be a multifunctional protein performing different roles in viral infection. The involvement of 33K protein in different steps of adenovirus replication may require protein-protein interaction. Using 33K protein as a bait in the yeast two-hybrid system, we screened open reading frames (ORFs) of bovine adenovirus (BAdV)-3 for potential interactions with 33K protein. Interestingly, 33K protein showed specific interaction with 100K and pV proteins. The yeast two-hybrid findings were validated by in vitro binding using in vitro synthesized transcription-translation products, GST pull down assay and in vitro co-immunoprecipitation assay using protein-specific antibodies. We demonstrated, that the interaction of 33K protein with 100K and pV proteins takes place during BAdV-3 infection. Finally, our data suggests that the stretch of amino acids 81-120 and 161-200 in 33K protein are critical for the interaction with pV and 100K proteins, respectively.
Kulshreshtha
Vikas
V
Vaccine and Infectious Disease Organization (VIDO), 120 Veterinary Road, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E3.
Tikoo
Suresh K
SK
D000257:Adenoviridae Infections / Q000821:virology*; D000818:Animals; D002417:Cattle; D002460:Cell Line; D017921:Mastadenovirus / Q000378:metabolism / Q000502:physiology*; D016366:Open Reading Frames / Q000235:genetics; D054730:Protein Interaction Domains and Motifs; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D014764:Viral Proteins