Application of the immunoperoxidase technique FDR the detection of Derzsy's disease virus antigen in cell culture and goslings.
Application of a direct immunoperoxidase technique for the detection of Derzsy's disease virus antigen in cell culture and goslings is described. Anti-virus globulins were labelled with horseradish peroxidase by use of sodium periodate as a coupling agent. Virus antigen was detected in the nuclei of goose embryo fibroblasts from 48 to 72 hours post-inoculation and in the nuclei of hepatic cells between 5 to 8 days after infection. Microstructural detail was much better in cell culture than in the liver.
Roszkowski
J
J
Veterinary Research Institute, 24-100 Pulawy, Poland.
Gaździński
P
P
Kozaczyński
W
W
Bartoszcze
M
M