title: Evaluation of rapid assays for the detection of bovine coronavirus, rotavirus A and Cryptosporidium parvum in faecal samples of calves.
abstract: Rapid immunochromatographic assays for detecting infections with bovine coronavirus (BCV), rotavirus A and Cryptosporidium parvum in calf faeces were evaluated using as gold standards a reverse transcriptase polymerase chain reaction (BCV and rotavirus) and a sedimentation-flotation technique (C. parvum). Rapid tests for the detection of BCV and rotavirus showed a high specificity (96.4% and 95.3%, respectively), but a relatively low sensitivity (60.0% and 71.9%, respectively). Sensitivity and specificity for detection of C. parvum were high (100% and 94.6%, respectively).
authors.affiliation: Clinic for Ruminants, Department for Farm Animals and Herd Management, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria. Daniela.Klein@vu-wien.ac.at
authors.forename: Daniela
authors.initials: D
authors.lastname: Klein
authors.forename: Angela
authors.initials: A
authors.lastname: Kern
authors.forename: Gudrun
authors.initials: G
authors.lastname: Lapan
authors.forename: Viviane
authors.initials: V
authors.lastname: Benetka
authors.forename: Karin
authors.initials: K
authors.lastname: Möstl
authors.forename: Andreas
authors.initials: A
authors.lastname: Hassl
authors.forename: Walter
authors.initials: W
authors.lastname: Baumgartner
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000469:parasitology / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000821:virology; D002845:Chromatography; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary; D017938:Coronavirus, Bovine / Q000302:isolation & purification*; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000662:veterinary; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology* / Q000821:virology*; D007118:Immunoassay / Q000379:methods / Q000592:standards / Q000662:veterinary; D010270:Parasite Egg Count / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000662:veterinary; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000662:veterinary; D012680:Sensitivity and Specificity; D013997:Time Factors
references.citation: J Clin Microbiol. 1984 Jun;19(6):952-3
references.pmid: 6206090
references.citation: J Vet Med Sci. 1991 Jun;53(3):433-7
references.pmid: 1653037
references.citation: J Vet Med B Infect Dis Vet Public Health. 2006 May;53(4):160-5
references.pmid: 16629982
references.citation: Parasitology. 1998 Jul;117 ( Pt 1):31-7
references.pmid: 9695098
references.citation: J Parasitol. 1997 Oct;83(5):825-30
references.pmid: 9379285
references.citation: Schweiz Arch Tierheilkd. 2005 May;147(6):245-52
references.pmid: 15999634
references.citation: Appl Environ Microbiol. 1996 Feb;62(2):643-7
references.pmid: 8593065