Various spatiotemporal expression profiles of anther-expressed genes in rice.
The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation.
Hobo
Tokunori
T
Bioscience and Biotechnology Center, Nagoya University, Furocho, Chikusa, Nagoya, 464-8601 Japan.
Suwabe
Keita
K
Aya
Koichiro
K
Suzuki
Go
G
Yano
Kentaro
K
Ishimizu
Takeshi
T
Fujita
Masahiro
M
Kikuchi
Shunsuke
S
Hamada
Kazuki
K
Miyano
Masumi
M
Fujioka
Tomoaki
T
Kaneko
Fumi
F
Kazama
Tomohiko
T
Mizuta
Yoko
Y
Takahashi
Hirokazu
H
Shiono
Katsuhiro
K
Nakazono
Mikio
M
Tsutsumi
Nobuhiro
N
Nagamura
Yoshiaki
Y
Kurata
Nori
N
Watanabe
Masao
M
Matsuoka
Makoto
M
D016000:Cluster Analysis; D005718:Gametogenesis / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D007834:Lasers; D042282:Microdissection / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D011058:Pollen / Q000235:genetics* / Q000254:growth & development; D018749:RNA, Plant / Q000235:genetics
D018749:RNA, Plant
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