title: Analysis of nuclear DNA content and ploidy in higher plants.
abstract: This is the first of a series of units discussing the application of cytometry to plant material. Techniques commonly used for mammalian nuclei evaluation need considerable modification to be successful with plant material. David Galbraith and his colleagues bring together many years of knowledge in plant cytometry. Their unit provides detailed protocols on measuring DNA content, ploidy, and cell cycle status of plant tissue using both conventional laser based instruments as well as arc lamp cytometers. This unit provides an excellent starting point for those interested in doing cytometry with plants.
authors.affiliation: University of Arizona, Tucson, Arizona, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Galbraith
authors.forename: G M
authors.initials: GM
authors.lastname: Lambert
authors.forename: J
authors.initials: J
authors.lastname: Macas
authors.forename: J
authors.initials: J
authors.lastname: Dolezel
mesh_terms: D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000032:analysis*; D018744:DNA, Plant / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D018745:Genome, Plant; D007834:Lasers; D010944:Plants / Q000235:genetics*; D011003:Ploidies
chemical_list: D018744:DNA, Plant; D005456:Fluorescent Dyes; D004247:DNA