Assessment of viability, immunofluorescence, and DNA content.
This unit addresses the difficult and important area of differentiation between viable and nonviable cells, a question of particular interest for DNA studies. The basic protocol describes the determination of DNA content using pyronin Y combined with dead cell discrimination using 7-AAD. Alternate protocols present methods for DNA content and viability assessment and simultaneous surface or intracellular antigen expression.
Schmid
I
I
UCLA School of Medicine, Los Angeles, California, USA.
D000818:Animals; D000941:Antigens / Q000096:biosynthesis; D002469:Cell Separation; D002470:Cell Survival; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*
D000941:Antigens; D004247:DNA