title: Transcriptome analysis identifies genes with enriched expression in the mouse central extended amygdala.
abstract: The central extended amygdala (EAc) is an ensemble of highly interconnected limbic structures of the anterior brain, and forms a cellular continuum including the bed nucleus of the stria terminalis (BNST), the central nucleus of the amygdala (CeA) and the nucleus accumbens shell (AcbSh). This neural network is a key site for interactions between brain reward and stress systems, and has been implicated in several aspects of drug abuse. In order to increase our understanding of EAc function at the molecular level, we undertook a genome-wide screen (Affymetrix) to identify genes whose expression is enriched in the mouse EAc. We focused on the less-well known BNST-CeA areas of the EAc, and identified 121 genes that exhibit more than twofold higher expression level in the EAc compared with whole brain. Among these, 43 genes have never been described to be expressed in the EAc. We mapped these genes throughout the brain, using non-radioactive in situ hybridization, and identified eight genes with a unique and distinct rostro-caudal expression pattern along AcbSh, BNST and CeA. Q-PCR analysis performed in brain and peripheral organ tissues indicated that, with the exception of one (Spata13), all these genes are predominantly expressed in brain. These genes encode signaling proteins (Adora2, GPR88, Arpp21 and Rem2), a transcription factor (Limh6) or proteins of unknown function (Rik130, Spata13 and Wfs1). The identification of genes with enriched expression expands our knowledge of EAc at a molecular level, and provides useful information to toward genetic manipulations within the EAc.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, Département Neurobiologie et Génétique, Illkirch, France. jbecker@igbmc.u-strasbg.fr
authors.forename: J A J
authors.initials: JA
authors.lastname: Becker
authors.forename: K
authors.initials: K
authors.lastname: Befort
authors.forename: C
authors.initials: C
authors.lastname: Blad
authors.forename: D
authors.initials: D
authors.lastname: Filliol
authors.forename: A
authors.initials: A
authors.lastname: Ghate
authors.forename: D
authors.initials: D
authors.lastname: Dembele
authors.forename: C
authors.initials: C
authors.lastname: Thibault
authors.forename: M
authors.initials: M
authors.lastname: Koch
authors.forename: J
authors.initials: J
authors.lastname: Muller
authors.forename: A
authors.initials: A
authors.lastname: Lardenois
authors.forename: O
authors.initials: O
authors.lastname: Poch
authors.forename: B L
authors.initials: BL
authors.lastname: Kieffer
mesh_terms: D000679:Amygdala / Q000033:anatomy & histology / Q000502:physiology*; D000818:Animals; D001931:Brain Mapping; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D009419:Nerve Tissue Proteins
references.citation: Am J Psychiatry. 2007 Aug;164(8):1149-59
references.pmid: 17671276
references.citation: Oncogene. 2007 Dec 6;26(55):7620-267
references.pmid: 17599059
references.citation: Eur Neuropsychopharmacol. 2003 Dec;13(6):442-52
references.pmid: 14636960
references.citation: Mol Psychiatry. 2004 Nov;9(11):1007-29
references.pmid: 15314610
references.citation: Ann N Y Acad Sci. 1999 Jun 29;877:217-41
references.pmid: 10415652
references.citation: Apoptosis. 2006 Jul;11(7):1067-74
references.pmid: 16832713
references.citation: J Comp Neurol. 2008 Jan 1;506(1):46-74
references.pmid: 17990271
references.citation: J Neurosci. 2006 Sep 27;26(39):9881-91
references.pmid: 17005852
references.citation: Cereb Cortex. 2006 Jul;16 Suppl 1:i82-8
references.pmid: 16766712
references.citation: Neuroscience. 1988 Oct;27(1):1-39
references.pmid: 3059226
references.citation: Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10357-62
references.pmid: 16002470
references.citation: J Neurosci. 2004 Apr 14;24(15):3879-89
references.pmid: 15084669
references.citation: Cell. 2007 Oct 19;131(2):405-18
references.pmid: 17956739
references.citation: Diabetologia. 2005 Nov;48(11):2313-21
references.pmid: 16215705
references.citation: Bioinformatics. 2004 Jun 12;20(9):1453-4
references.pmid: 14871861
references.citation: Endocrinology. 2005 Oct;146(10):4391-8
references.pmid: 16002521
references.citation: Hum Mol Genet. 2006 Mar 1;15(5):691-703
references.pmid: 16434483
references.citation: Neurosci Lett. 2002 Apr 5;322(2):116-20
references.pmid: 11958857
references.citation: J Alzheimers Dis. 2007 Dec;12(4):291-311
references.pmid: 18198416
references.citation: Nature. 2007 Jan 11;445(7124):168-76
references.pmid: 17151600
references.citation: Genes Brain Behav. 2003 Oct;2(5):282-94
references.pmid: 14606693
references.citation: Eur J Neurosci. 2008 Jun;27(11):2973-84
references.pmid: 18588537
references.citation: J Comp Neurol. 1993 Apr 15;330(3):381-404
references.pmid: 8385679
references.citation: Neuropsychopharmacology. 2005 Jul;30(7):1302-11
references.pmid: 15812571
references.citation: Psychopharmacology (Berl). 2003 Jul;168(1-2):3-20
references.pmid: 12402102
references.citation: Methods. 2003 Dec;31(4):290-300
references.pmid: 14597313
references.citation: Neuropharmacology. 2006 Oct;51(5):1013-22
references.pmid: 16904135
references.citation: Mol Cell Biol. 2007 Feb;27(4):1380-93
references.pmid: 17145773
references.citation: Biochem J. 2000 Apr 1;347 Pt 1:223-31
references.pmid: 10727423
references.citation: Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5270-5
references.pmid: 11320257
references.citation: Eur J Pharmacol. 1993 Jan 26;231(1):139-42
references.pmid: 8444279
references.citation: Neuron. 2007 Jan 18;53(2):217-32
references.pmid: 17224404
references.citation: Bioinformatics. 2005 May 1;21(9):2095-6
references.pmid: 15647299
references.citation: J Neurosci. 2008 Jan 2;28(1):264-78
references.pmid: 18171944
references.citation: Brain Res. 2002 Jul 5;943(1):38-47
references.pmid: 12088837
references.citation: Hum Mol Genet. 2001 Mar 1;10(5):477-84
references.pmid: 11181571
references.citation: Hum Mol Genet. 2006 Mar 15;15(6):965-77
references.pmid: 16467349
references.citation: Nat Genet. 1998 Oct;20(2):143-8
references.pmid: 9771706
references.citation: Neuron. 2005 May 19;46(4):647-60
references.pmid: 15944132
references.citation: Hum Mol Genet. 2004 Jun 1;13(11):1159-70
references.pmid: 15056606
references.citation: J Neurosci. 2008 Feb 20;28(8):1854-64
references.pmid: 18287502
references.citation: Ann N Y Acad Sci. 2003 Apr;985:174-84
references.pmid: 12724158
references.citation: Brain Res Dev Brain Res. 1994 Dec 16;83(2):247-61
references.pmid: 7535204
references.citation: J Cell Physiol. 2005 Jan;202(1):9-20
references.pmid: 15389588
references.citation: Brain Res. 2006 Aug 30;1107(1):24-41
references.pmid: 16824496
references.citation: Rev Neurosci. 2005;16(4):287-302
references.pmid: 16519006
references.citation: J Neurosci. 2005 Oct 19;25(42):9762-72
references.pmid: 16237180
references.citation: Ann N Y Acad Sci. 2008;1129:175-84
references.pmid: 18591478
references.citation: Hum Mutat. 2003 Oct;22(4):275-87
references.pmid: 12955714
references.citation: J Neural Transm (Vienna). 2001;108(7):837-48
references.pmid: 11515749
references.citation: Neurosci Lett. 2007 Oct 16;426(2):87-90
references.pmid: 17884289
references.citation: Neuroscience. 2006;137(1):177-86
references.pmid: 16309847
references.citation: Neuroscience. 2007 May 25;146(3):1182-92
references.pmid: 17395390
references.citation: J Neurosci. 2007 Dec 12;27(50):13624-34
references.pmid: 18077674
references.citation: J Comp Neurol. 2008 Mar 10;507(2):1245-57
references.pmid: 18181142
references.citation: Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
references.pmid: 9843981
references.citation: J Comp Neurol. 1987 Apr 8;258(2):159-84
references.pmid: 3584538
references.citation: Curr Opin Neurobiol. 2002 Oct;12(5):562-5
references.pmid: 12367636
references.citation: Nature. 2003 Oct 30;425(6961):917-25
references.pmid: 14586460
references.citation: J Neurosci. 1999 Jul 1;19(13):5409-19
references.pmid: 10377350
references.citation: J Neurosci. 2007 Nov 7;27(45):12255-66
references.pmid: 17989291
references.citation: Genome Res. 2003 Jul;13(7):1646-53
references.pmid: 12840043
references.citation: Genomics. 2000 Nov 1;69(3):314-21
references.pmid: 11056049
references.citation: BMC Genomics. 2007 Apr 04;8:94
references.pmid: 17408481
references.citation: Neuropsychopharmacology. 2004 Mar;29(3):558-65
references.pmid: 14666117
references.citation: J Neurosci. 1989 Mar;9(3):865-75
references.pmid: 2538585
references.citation: Neurosci Lett. 2005 Apr 18;378(2):98-101
references.pmid: 15774265
references.citation: Neuroscience. 1990;37(2):317-25
references.pmid: 1966823
references.citation: Ann N Y Acad Sci. 1999 Jun 29;877:1-32
references.pmid: 10415640
references.citation: J Neurochem. 1993 Mar;60(3):1043-6
references.pmid: 8436957
references.citation: Peptides. 1989 May-Jun;10(3):687-92
references.pmid: 2780423
references.citation: J Neurosci. 2005 Apr 20;25(16):4181-8
references.pmid: 15843621
references.citation: Physiol Genomics. 2008 Feb 19;32(3):283-98
references.pmid: 17911379