title: B-cell clonal diversification and gut-lymph node trafficking in ulcerative colitis revealed using lineage tree analysis.
abstract: In studies of inflammatory bowel diseases (IBD), research has so far focused mainly on the role of T cells. Despite evidence suggesting that B cells and the production of autoantibodies may play a significant role in IBD pathogenesis, the role of B cells in gut inflammation has not yet been thoroughly investigated. In the present study we used the new approach of lineage tree analysis for studying immunoglobulin variable region gene diversification in B cells found in the inflamed intestinal tissue of two ulcerative colitis patients as well as B cells from mucosa-associated lymph nodes (LN) in the same patients. Healthy intestinal tissue of three patients with carcinoma of the colon was used as normal control. Lineage tree shapes revealed active immune clonal diversification processes occurring in ulcerative colitis patients, which were quantitatively similar to those in healthy controls. B cells from intestinal tissues and the associated LN are shown here to be clonally related, thus supplying the first direct evidence supporting B-cell trafficking between gut and associated LN in IBD and control tissues.
authors.affiliation: The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Hilla
authors.initials: H
authors.lastname: Tabibian-Keissar
authors.forename: Neta S
authors.initials: NS
authors.lastname: Zuckerman
authors.forename: Michal
authors.initials: M
authors.lastname: Barak
authors.forename: Deborah K
authors.initials: DK
authors.lastname: Dunn-Walters
authors.forename: Avital
authors.initials: A
authors.lastname: Steiman-Shimony
authors.forename: Yehuda
authors.initials: Y
authors.lastname: Chowers
authors.forename: Efrat
authors.initials: E
authors.lastname: Ofek
authors.forename: Kinneret
authors.initials: K
authors.lastname: Rosenblatt
authors.forename: Ginette
authors.initials: G
authors.lastname: Schiby
authors.forename: Ramit
authors.initials: R
authors.lastname: Mehr
authors.forename: Iris
authors.initials: I
authors.lastname: Barshack
mesh_terms: D000328:Adult; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000276:immunology*; D002465:Cell Movement; D003093:Colitis, Ulcerative / Q000235:genetics / Q000276:immunology*; D003106:Colon / Q000276:immunology*; D005260:Female; D005803:Genes, Immunoglobulin; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology*; D008297:Male; D008875:Middle Aged
chemical_list: D007135:Immunoglobulin Variable Region