title: A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance.
abstract: Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation.
authors.affiliation: Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Jia-Shu
authors.initials: JS
authors.lastname: Yang
authors.forename: Helge
authors.initials: H
authors.lastname: Gad
authors.forename: Stella Y
authors.initials: SY
authors.lastname: Lee
authors.forename: Alexander
authors.initials: A
authors.lastname: Mironov
authors.forename: Leiliang
authors.initials: L
authors.lastname: Zhang
authors.forename: Galina V
authors.initials: GV
authors.lastname: Beznoussenko
authors.forename: Carmen
authors.initials: C
authors.lastname: Valente
authors.forename: Gabriele
authors.initials: G
authors.lastname: Turacchio
authors.forename: Akua N
authors.initials: AN
authors.lastname: Bonsra
authors.forename: Guangwei
authors.initials: G
authors.lastname: Du
authors.forename: Gianluca
authors.initials: G
authors.lastname: Baldanzi
authors.forename: Andrea
authors.initials: A
authors.lastname: Graziani
authors.forename: Sylvain
authors.initials: S
authors.lastname: Bourgoin
authors.forename: Michael A
authors.initials: MA
authors.lastname: Frohman
authors.forename: Alberto
authors.initials: A
authors.lastname: Luini
authors.forename: Victor W
authors.initials: VW
authors.lastname: Hsu
mesh_terms: D000818:Animals; D022181:COP-Coated Vesicles / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D002522:Chlorocebus aethiops; D006056:Golgi Apparatus / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D051379:Mice; D010712:Phosphatidic Acids / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D002352:Carrier Proteins; D008081:Liposomes; D010712:Phosphatidic Acids; C105606:phospholipase D2; D010739:Phospholipase D
references.citation: Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13676-80
references.pmid: 9811859
references.citation: Nature. 1999 Nov 25;402(6760):429-33
references.pmid: 10586885
references.citation: Curr Opin Cell Biol. 2003 Aug;15(4):372-81
references.pmid: 12892776
references.citation: Annu Rev Cell Dev Biol. 2006;22:439-55
references.pmid: 16824007
references.citation: J Cell Biol. 1996 Jul;134(2):295-306
references.pmid: 8707816
references.citation: J Biol Chem. 1985 Dec 15;260(29):15762-70
references.pmid: 2999135
references.citation: J Cell Biol. 2005 Jan 17;168(2):281-90
references.pmid: 15657398
references.citation: Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8253-7
references.pmid: 12832619
references.citation: Cell. 1991 Mar 22;64(6):1183-95
references.pmid: 2004424
references.citation: Nature. 1993 Aug 19;364(6439):732-4
references.pmid: 8355790
references.citation: Nat Rev Mol Cell Biol. 2005 Oct;6(10):812-7
references.pmid: 16167055
references.citation: Curr Opin Cell Biol. 2007 Aug;19(4):426-35
references.pmid: 17651957
references.citation: EMBO J. 2003 Aug 15;22(16):4059-69
references.pmid: 12912905
references.citation: Nature. 1992 Nov 26;360(6402):350-2
references.pmid: 1448151
references.citation: Nature. 2005 Dec 1;438(7068):675-8
references.pmid: 16319893
references.citation: Nat Rev Mol Cell Biol. 2006 Jan;7(1):9-19
references.pmid: 16365634
references.citation: Nature. 1991 Jan 17;349(6306):248-51
references.pmid: 1898986
references.citation: Nat Cell Biol. 2004 Nov;6(11):1071-81
references.pmid: 15502824
references.citation: Cell. 2005 Aug 26;122(4):605-17
references.pmid: 16122427
references.citation: Curr Opin Cell Biol. 2003 Aug;15(4):396-404
references.pmid: 12892779
references.citation: Nat Cell Biol. 2006 Dec;8(12):1376-82
references.pmid: 17086176
references.citation: Mol Biol Cell. 2007 Sep;18(9):3250-63
references.pmid: 17567948
references.citation: Cell. 1991 Oct 18;67(2):239-53
references.pmid: 1680566
references.citation: Mol Biol Cell. 2004 Oct;15(10):4710-24
references.pmid: 15282336
references.citation: Nat Cell Biol. 2005 Jun;7(6):570-80
references.pmid: 15880102
references.citation: Oncogene. 2008 Feb 7;27(7):942-56
references.pmid: 17700527
references.citation: Nature. 1992 Nov 26;360(6402):352-4
references.pmid: 1448152
references.citation: Cell. 1999 Feb 19;96(4):495-506
references.pmid: 10052452
references.citation: Mol Biol Cell. 2001 Apr;12(4):943-55
references.pmid: 11294898
references.citation: Mol Biol Cell. 2002 Nov;13(11):3930-42
references.pmid: 12429836
references.citation: EMBO J. 2005 Dec 7;24(23):4133-43
references.pmid: 16292346
references.citation: FASEB J. 1994 Aug;8(11):879-83
references.pmid: 8070638
references.citation: J Cell Biol. 1994 Jun;125(6):1213-24
references.pmid: 8207054
references.citation: J Cell Biol. 2002 Oct 14;159(1):69-78
references.pmid: 12379802
references.citation: Annu Rev Cell Dev Biol. 2004;20:87-123
references.pmid: 15473836
references.citation: Cell. 1990 Mar 9;60(5):821-36
references.pmid: 2178778
references.citation: Nature. 2002 May 9;417(6885):187-93
references.pmid: 12000962
references.citation: Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11199-204
references.pmid: 9736713
references.citation: Nature. 2005 Dec 1;438(7068):590-6
references.pmid: 16319878
references.citation: Nat Rev Mol Cell Biol. 2006 May;7(5):347-58
references.pmid: 16633337
references.citation: Biochem J. 2008 Jan 1;409(1):1-18
references.pmid: 18062770
references.citation: Nat Cell Biol. 2004 May;6(5):393-404
references.pmid: 15107860
references.citation: J Cell Biol. 2001 Oct 15;155(2):193-200
references.pmid: 11604418
references.citation: Annu Rev Biochem. 1997;66:475-509
references.pmid: 9242915
references.citation: Cell Mol Life Sci. 2005 Oct;62(19-20):2305-16
references.pmid: 16143829