The effect of temperature on mechanical resistance of the native and intermediate states of I27.
We investigated the effect of temperature on the mechanical unfolding of I27 from human cardiac titin, employing a custom-built temperature control device for single-molecule atomic force microscopy measurement. A sawtooth pattern was observed in the force curves where each force peak reports on the unfolding of an I27 domain. In early unfolding events, we observed a "hump-like" deviation due to the detachment of beta-strand A from each I27 domain. The force at which the humps appear was approximately 130 pN and showed no temperature dependence, at least in the temperature range of 2 degrees C-30 degrees C. The hump structure was successfully analyzed with a two-state worm-like chain model, and the Gibbs free energy difference of the detachment reaction was estimated to be 11.6 +/- 0.58 kcal/mol and found to be temperature independent. By contrast, upon lowering the temperature, the mean unfolding force from the partly unfolded intermediate state was found to markedly increase and the unfolding force distribution to broaden significantly, suggesting that the distance (x(u)) between the folded and transition states in the energy landscape along the pulling direction is decreased. These results suggest that the local structure of beta-strand A are stabilized by topologically simple local hydrogen-bond network and that the temperature does not affect the detachment reaction thermodynamically and kinetically, whereas the interaction between the beta-strands A' and G, which is a critical region for its mechanical stability, is strongly dependent on the temperature.
Taniguchi
Yukinori
Y
School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa, Japan.
Brockwell
David J
DJ
Kawakami
Masaru
M
D064211:Connectin; D018625:Microscopy, Atomic Force; D009124:Muscle Proteins / Q000737:chemistry*; D011489:Protein Denaturation; D011494:Protein Kinases / Q000737:chemistry*; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D013314:Stress, Mechanical; D013696:Temperature; D013816:Thermodynamics
D064211:Connectin; D009124:Muscle Proteins; C577285:TTN protein, human; D011494:Protein Kinases
Nature. 1999 Nov 4;402(6757):100-3
10573426
Biophys J. 2003 Nov;85(5):3286-93
14581229
J Mol Recognit. 2006 Sep-Oct;19(5):389-407
16897812
Nat Struct Biol. 2000 Dec;7(12):1117-20
11101892
J Mol Biol. 2005 Nov 25;354(2):497-503
16246362
Circ Res. 2001 Nov 23;89(11):1065-72
11717165
Biophys J. 2006 Jul 15;91(2):467-72
16632514
Biophys J. 2005 Jul;89(1):506-19
15863479
J Am Chem Soc. 2007 Jan 17;129(2):246-7
17212383
Biophys J. 2007 Aug 1;93(3):930-7
17483176
Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10249-53
12934020
Proteins. 2004 Aug 1;56(2):285-97
15211512
Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4451-6
16537423
Biophys J. 1998 Aug;75(2):662-71
9675168
Protein Sci. 2002 Dec;11(12):2759-65
12441375
J Mol Recognit. 2007 Nov-Dec;20(6):495-501
17902095
Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3185-90
18305176
Biophys J. 2004 Dec;87(6):3995-4006
15361414
J Phys Chem A. 2007 Dec 13;111(49):12402-8
18020430
Electrophoresis. 1997 Dec;18(15):2714-23
9504803
J Phys Chem B. 2007 Dec 6;111(48):13653-7
17988116
EMBO Rep. 2005 May;6(5):482-6
15864299
Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):139-44
10618384
EMBO J. 2003 Oct 1;22(19):5220-9
14517259
Biophys J. 2006 Jul 15;91(2):L16-8
16698787
Curr Opin Struct Biol. 2004 Feb;14(1):70-5
15102452
Nat Struct Biol. 2003 Sep;10(9):731-7
12923573
Pflugers Arch. 2008 Apr;456(1):101-15
18058125
Chem Commun (Camb). 2002 Feb 7;(3):183-92
12120362
J Mol Biol. 2008 Jan 4;375(1):316-24
18021802
Nat Mater. 2002 Dec;1(4):232-5
12618784
Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9633-7
17535921
Biophys J. 1997 Apr;72(4):1541-55
9083660
Structure. 1996 Mar 15;4(3):323-37
8805538
J Mol Biol. 2002 Sep 27;322(4):841-9
12270718
Curr Opin Struct Biol. 2007 Feb;17(1):58-66
17251000
J Mol Biol. 2003 Jul 18;330(4):867-77
12850153
Nature. 2003 Mar 27;422(6930):446-9
12660787
Science. 2007 May 25;316(5828):1144-8
17525328
Science. 1991 Dec 13;254(5038):1598-603
1749933
Biophys J. 2002 Jul;83(1):458-72
12080133
Biophys J. 2007 Mar 15;92(6):1825-35
17158578
Chemphyschem. 2006 Aug 11;7(8):1710-6
16865759
Nat Struct Mol Biol. 2004 Jan;11(1):81-5
14718927
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3694-9
10097099
Phys Rev E Stat Nonlin Soft Matter Phys. 2007 Feb;75(2 Pt 1):021907
17358367