The structure of YqeH. An AtNOS1/AtNOA1 ortholog that couples GTP hydrolysis to molecular recognition.
AtNOS1/AtNOA1 was identified as a nitric oxide-generating enzyme in plants, but that function has recently been questioned. To resolve issues surrounding AtNOA1 activity, we report the biochemical properties and a 2.36 A resolution crystal structure of a bacterial AtNOA1 ortholog (YqeH). Geobacillus YqeH fused to a putative AtNOA1 leader peptide complements growth and morphological defects of Atnoa1 mutant plants. YqeH does not synthesize nitric oxide from L-arginine but rather hydrolyzes GTP. The YqeH structure reveals a circularly permuted GTPase domain and an unusual C-terminal beta-domain. A small N-terminal domain, disordered in the structure, binds zinc. Structural homology among the C-terminal domain, the RNA-binding regulator TRAP, and the hypoxia factor pVHL define a recognition module for peptides and nucleic acids. TRAP residues important for RNA binding are conserved by the YqeH C-terminal domain, whose positioning is coupled to GTP hydrolysis. YqeH and AtNOA1 probably act as G-proteins that regulate nucleic acid recognition and not as nitric-oxide synthases.
Sudhamsu
Jawahar
J
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
Lee
Gyu In
GI
Klessig
Daniel F
DF
Crane
Brian R
BR
D020816:Amino Acid Motifs; D029681:Arabidopsis Proteins / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology*; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D020558:GTP Phosphohydrolases / Q000737:chemistry; D006160:Guanosine Triphosphate / Q000737:chemistry*; D006868:Hydrolysis; D008958:Models, Molecular; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000737:chemistry; D019001:Nitric Oxide Synthase / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D029681:Arabidopsis Proteins; D009569:Nitric Oxide; D006160:Guanosine Triphosphate; C521947:NOA1 protein, Arabidopsis; D019001:Nitric Oxide Synthase; D020558:GTP Phosphohydrolases
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