title: Chromosomal regions underlying noncoagulation of milk in Finnish Ayrshire cows.
abstract: About 10% of Finnish Ayrshire cows produce noncoagulating milk, i.e., milk that does not form a curd in a standard 30-min testing time and is thus a poor raw material for cheese dairies. This phenomenon is associated with peak and midlactation, but some cows produce noncoagulating milk persistently. A genomewide scan under a selective DNA pooling method was carried out to locate genomic regions associated with the noncoagulation of milk. On the basis of the hypothesis of the same historical mutation, we pooled the data across sires. Before testing pools for homogeneity, allele intensities were corrected for PCR artifacts, i.e., shadow bands and differential amplification. Results indicating association were verified using daughter design and selective genotyping within families. Data consisted of 18 sire families with 477 genotyped daughters in total, i.e., 12% of each tail of the milk coagulation ability. Data were analyzed using interval mapping under maximum-likelihood and nonparametric methods. BMS1126 on chromosome 2 and BMS1355 on chromosome 18 were associated with noncoagulation of milk across families on an experimentwise 0.1% significance level. By scanning gene databases, we found two potential candidate genes: LOC538897, a nonspecific serine/threonine kinase on chromosome 2, and SIAT4B, a sialyltransferase catalyzing the last step of glycosylation of kappa-casein on chromosome 18. Further studies to determine the role of the candidates in the noncoagulation of milk are clearly needed.
authors.affiliation: Department of Animal Science, University of Helsinki, FI-00014 Helsinki, Finland. anna-maria.tyriseva@helsinki.fi
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Tyrisevä
authors.forename: Kari
authors.initials: K
authors.lastname: Elo
authors.forename: Arja
authors.initials: A
authors.lastname: Kuusipuro
authors.forename: Veijo
authors.initials: V
authors.lastname: Vilva
authors.forename: Isto
authors.initials: I
authors.lastname: Jänönen
authors.forename: Heidi
authors.initials: H
authors.lastname: Karjalainen
authors.forename: Tiina
authors.initials: T
authors.lastname: Ikonen
authors.forename: Matti
authors.initials: M
authors.lastname: Ojala
mesh_terms: D000818:Animals; D001947:Breeding; D002364:Caseins / Q000235:genetics; D002417:Cattle / Q000235:genetics*; D002611:Cheese; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics*; D005260:Female; D005387:Finland; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002364:Caseins
references.citation: Heredity (Edinb). 1999 Nov;83 (Pt 5):613-24
references.pmid: 10620035
references.citation: J Dairy Sci. 2006 Sep;89(9):3296-305
references.pmid: 16899662
references.citation: J Dairy Res. 2007 May;74(2):167-73
references.pmid: 17291390
references.citation: J Dairy Sci. 1999 Jan;82(1):205-14
references.pmid: 10022022
references.citation: J Dairy Sci. 2004 Feb;87(2):458-67
references.pmid: 14762089
references.citation: J Dairy Sci. 2001 May;84(5):1027-33
references.pmid: 11384028
references.citation: Genetics. 2003 Mar;163(3):1169-75
references.pmid: 12663553
references.citation: Genetics. 1995 Mar;139(3):1421-8
references.pmid: 7768449
references.citation: Proteomics. 2006 May;6(10):3087-95
references.pmid: 16619295
references.citation: Cell Mol Life Sci. 2006 Feb;63(3):378-89
references.pmid: 16429323
references.citation: Genetics. 1994 Dec;138(4):1365-73
references.pmid: 7896115
references.citation: Theor Appl Genet. 1990 Nov;80(5):631-4
references.pmid: 24221069
references.citation: J Dairy Sci. 2008 Jan;91(1):371-6
references.pmid: 18096961
references.citation: Theor Appl Genet. 1992 Nov;85(2-3):353-9
references.pmid: 24197326
references.citation: Vet Rec. 1977 Oct 15;101(16):325-6
references.pmid: 563124
references.citation: Biotechniques. 1991 Apr;10(4):506-13
references.pmid: 1867860
references.citation: J Dairy Res. 1997 May;64(2):231-8
references.pmid: 9161915
references.citation: Ann Hum Genet. 1995 Jan;59(1):97-105
references.pmid: 7762987
references.citation: J Dairy Sci. 2004 Nov;87(11):3958-66
references.pmid: 15483181
references.citation: Genome Res. 2000 Jan;10(1):105-15
references.pmid: 10645955
references.citation: J Dairy Res. 2003 Feb;70(1):91-8
references.pmid: 12617397
references.citation: Genetics. 1998 Jul;149(3):1547-55
references.pmid: 9649541
references.citation: Genetics. 1998 Jul;149(3):1557-67
references.pmid: 9649542
references.citation: Nucleic Acids Res. 1988 Feb 11;16(3):1215
references.pmid: 3344216
references.citation: J Dairy Res. 2006 May;73(2):171-7
references.pmid: 16476179
references.citation: Genetics. 1995 Feb;139(2):907-20
references.pmid: 7713441
references.citation: J Dairy Sci. 2004 Feb;87(2):443-9
references.pmid: 14762087
references.citation: J Dairy Sci. 1985 Oct;68(10):2527-33
references.pmid: 3934240
references.citation: Genetics. 2007 Nov;177(3):1963-6
references.pmid: 17720902
references.citation: J Dairy Sci. 1993 Jul;76(7):2025-32
references.pmid: 8345129
references.citation: J Dairy Sci. 1991 Sep;74(9):2879-87
references.pmid: 1779047