Host ranges of Listeria-specific bacteriophages from the turkey processing plant environment in the United States.
Even though at least 400 Listeria phages have been isolated from various sources, limited information is available on phages from the food processing plant environment. Phages in the processing plant environment may play critical roles in determining the Listeria population that becomes established in the plant. In this study, we pursued the isolation of Listeria-specific phages from environmental samples from four turkey processing plants in the United States. These environmental samples were also utilized to isolate Listeria spp. Twelve phages were isolated and classified into three groups in terms of their host range. Of these, nine (group 1) showed a wide host range, including multiple serotypes of Listeria monocytogenes, as well as other Listeria spp. (L. innocua, L. welshimeri, L. seeligeri, and L. ivanovii). The remaining phages mostly infected L. monocytogenes serotype 4b as well as L. innocua, L. ivanovii, and/or L. welshimeri. All but one of the strains of the serotype 4b complex (4b, 4d, 4e) from the processing plant environment could be readily infected by the wide-host-range phages isolated from the environment of the processing plants. However, many strains of other serotypes (1/2a [or 3a] and 1/2b [or 3b]), which represented the majority of L. monocytogenes strains isolated from the environmental samples, were resistant to infection by these phages. Experiments with two phage-resistant strains showed reduced phage adsorption onto the host cells. These findings suggest that phage resistance may be an important component of the ecology of L. monocytogenes in the turkey processing plants.
Kim
Jae-Won
JW
Department of Food Science, North Carolina State University, Raleigh, NC 27695-7624, USA.
Siletzky
Robin M
RM
Kathariou
Sophia
S
D000818:Animals; D001435:Bacteriophages / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D019649:Food Industry; D005516:Food Microbiology; D008087:Listeria / Q000821:virology*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014422:Turkeys; D014481:United States; D053585:Virus Attachment
D004279:DNA, Viral
Appl Environ Microbiol. 1990 Jun;56(6):1912-8
2116763
Appl Environ Microbiol. 2003 Dec;69(12):7336-42
14660383
Appl Environ Microbiol. 2008 Mar;74(5):1464-8
18192428
Fed Regist. 2016 Apr 15;81(73):22176-83
27101640
Int J Food Microbiol. 2006 Nov 1;112(2):153-61
16843563
Microbiology (Reading). 1996 Apr;142 ( Pt 4):985-992
8936325
Foodborne Pathog Dis. 2005 Fall;2(3):192-200
16156700
Appl Environ Microbiol. 2000 Nov;66(11):5083-6
11055967
Appl Environ Microbiol. 2008 Mar;74(5):1653-5
18192405
Mol Microbiol. 2000 Jan;35(2):312-23
10652092
Appl Environ Microbiol. 2006 Jul;72(7):5073-6
16820508
Regul Toxicol Pharmacol. 2005 Dec;43(3):301-12
16188359
Nucleic Acids Res. 2004 Apr 28;32(8):2386-95
15115801
Lett Appl Microbiol. 2005;41(3):291-6
16108923
Int J Food Microbiol. 1989 Jun;8(3):251-7
2518382
J Food Prot. 2004 Aug;67(8):1682-6
15330534
Appl Environ Microbiol. 1995 Jun;61(6):2139-44
7793936
J Appl Bacteriol. 1996 Apr;80(4):395-401
8849641
Infect Immun. 2001 Apr;69(4):2054-65
11254558
J Food Prot. 2005 Feb;68(2):426-37
15726992
Appl Environ Microbiol. 1991 Mar;57(3):882-4
2039238
J Clin Microbiol. 2004 Aug;42(8):3819-22
15297538
J Food Prot. 2002 Nov;65(11):1811-29
12430709
Intervirology. 1994;37(1):31-5
7928287
Appl Environ Microbiol. 2003 Aug;69(8):4519-26
12902237