title: Reduction of blood coagulation and monocyte-platelet interaction following the use of a minimal extracorporeal circulation system (Synergy) in coronary artery bypass grafting (CABG).
abstract: Cardiovascular surgery with cardiopulmonary bypass (CPB) induces activation of blood coagulation and systemic inflammation involved in post-operative complications. Our study evaluated the impact of the minimal extracorporeal circulation (mini-CPB) system (Synergy, Sorin Group) on these functional aspects. Twenty patients were randomly assigned to standard CPB (n = 10) or to Synergy (n = 10). Platelet expression of PAC-1, and monocyte/granulocyte-platelet conjugates were evaluated by flow cytometry. A leukocyte-platelet adhesion index was calculated after cell number normalization. ELISAs were performed to measure IL-6 and TNF-alpha, thrombin-antithrombin III complexes (TAT), prothrombin fragments (F1+2), beta-thromboglobulin (beta-TG) and sP-selectin (sCD62P). Blood samples were drawn at the time of anesthesia (T1), at the end of CPB (T2), and at 4 (T3) and 24 hours (T4) after weaning from CPB. All patients were similar for clinical characteristics. When compared to standard CPB, the Synergy showed lower levels of the monocyte-platelet adhesion index at T2 (0.023 +/- 0.005 vs 0.063 +/- 0.013, P = 0.0092) and T4 (0.031 +/- 0.003 vs 0.055 +/- 0.005, P = 0.0017), TAT complexes at T2 (27.175 +/- 5.967 vs 86.592 +/- 5.415, P = 0.0005) and T3 (26.977 +/- 2.468 vs 45.146 +/- 4.365, P = 0.0041), F1+2 fragments at T2 (2.222 +/- 0.226 vs 4.249 +/- 0.292, P = 0.0009), and sP-selectin at T3 (115.17 +/- 19.623 vs 169.554 +/- 19.709, P = 0.0703) and T4 (108.542 +/- 6.429 vs 140.799 +/- 14.771, P = 0.0833). In summary, the Synergy exhibited a lower post-operative activation of blood coagulation, together with a reduced interaction between circulating monocytes and platelets.
authors.affiliation: Cardiac Surgery Department, "G. Pasquinucci" Hospital, Massa (MS), Italy.
authors.forename: P A
authors.initials: PA
authors.lastname: Farneti
authors.forename: S
authors.initials: S
authors.lastname: Sbrana
authors.forename: D
authors.initials: D
authors.lastname: Spiller
authors.forename: A G
authors.initials: AG
authors.lastname: Cerillo
authors.forename: F
authors.initials: F
authors.lastname: Santarelli
authors.forename: D
authors.initials: D
authors.lastname: Di Dario
authors.forename: P A
authors.initials: PA
authors.lastname: Del Sarto
authors.forename: M
authors.initials: M
authors.lastname: Glauber
mesh_terms: D000368:Aged; D001777:Blood Coagulation; D001792:Blood Platelets / Q000502:physiology; D001026:Coronary Artery Bypass / Q000295:instrumentation / Q000379:methods; D005112:Extracorporeal Circulation / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology; D019007:P-Selectin / Q000097:blood
chemical_list: D019007:P-Selectin