title: Adult SVZ stem cells lie in a vascular niche: a quantitative analysis of niche cell-cell interactions.
abstract: There is an emerging understanding of the importance of the vascular system within stem cell niches. Here, we examine whether neural stem cells (NSCs) in the adult subventricular zone (SVZ) lie close to blood vessels, using three-dimensional whole mounts, confocal microscopy, and automated computer-based image quantification. We found that the SVZ contains a rich plexus of blood vessels that snake along and within neuroblast chains. Cells expressing stem cell markers, including GFAP, and proliferation markers are closely apposed to the laminin-containing extracellular matrix (ECM) surrounding vascular endothelial cells. Apical GFAP+ cells are admixed within the ependymal layer and some span between the ventricle and blood vessels, occupying a specialized microenvironment. Adult SVZ progenitor cells express the laminin receptor alpha6beta1 integrin, and blocking this inhibits their adhesion to endothelial cells, altering their position and proliferation in vivo, indicating that it plays a functional role in binding SVZ stem cells within the vascular niche.
authors.affiliation: New York Neural Stem Cell Institute, Rensselaer, NY 12144, USA.
authors.forename: Qin
authors.initials: Q
authors.lastname: Shen
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Erzsebet
authors.initials: E
authors.lastname: Kokovay
authors.forename: Gang
authors.initials: G
authors.lastname: Lin
authors.forename: Shu-Mien
authors.initials: SM
authors.lastname: Chuang
authors.forename: Susan K
authors.initials: SK
authors.lastname: Goderie
authors.forename: Badrinath
authors.initials: B
authors.lastname: Roysam
authors.forename: Sally
authors.initials: S
authors.lastname: Temple
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D002450:Cell Communication; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D039121:Integrin alpha6beta1 / Q000378:metabolism; D020547:Lateral Ventricles / Q000098:blood supply / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D005904:Glial Fibrillary Acidic Protein; D039121:Integrin alpha6beta1; D049452:Green Fluorescent Proteins
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