title: Chiral separation of fluvastatin enantiomers by capillary electrophoresis.
abstract: An analytical CE method was developed for the enantiomeric purity determination of fluvastatin enantiomers. Fluvastatin enantiomers were separated on an uncoated fused silica with 100 mM-borate solution containing 30 mg/mL of (2-hydroxypropyl)-beta-cyclodextrin (HP-beta-CD) as running buffer and fenoprofen as an internal standard. The linearity was observed within a 400-700 microg/mL concentration range (r(2)>or=0.995) for both fluvastatin enantiomers. The repeatability expressed as coefficient of variation (CV) of the method were 0.96 and 0.92% for (+)-3R, 5S and (-)-3S, 5R-fluvastatin, respectively. The limit of detection and quantification for both fluvastatin enantiomers were 1.5 microg/mL and 2.5 microg/mL, respectively.
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Tran Quoc
authors.initials: TQ
authors.lastname: Trung
authors.forename: Phan Thanh
authors.initials: PT
authors.lastname: Dung
authors.forename: Nguyen Ngoc
authors.initials: NN
authors.lastname: Hoan
authors.forename: Dae Joong
authors.initials: DJ
authors.lastname: Kim
authors.forename: Joo Huyn
authors.initials: JH
authors.lastname: Lee
authors.forename: Kyeong Ho
authors.initials: KH
authors.lastname: Kim
mesh_terms: D053801:Capillary Electrochromatography; D003505:Cyclodextrins; D004574:Electromagnetic Fields; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000302:isolation & purification*; D000077340:Fluvastatin; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D003505:Cyclodextrins; D005229:Fatty Acids, Monounsaturated; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007211:Indoles; D012996:Solutions; D000077340:Fluvastatin