Chondroitin sulfate extracted from ascidian tunic inhibits phorbol ester-induced expression of Inflammatory factors VCAM-1 and COX-2 by blocking NF-kappaB activation in mouse skin.
Inflammatory factors are known to play a key role in promoting tumorigenesis; therefore, it is a promising strategy to inhibit the inflammation for cancer prevention. The current study was performed to investigate the potential effects of chondroitin sulfate (CS) extracted from ascidian tunic on the expression of inflammatory factors induced by treatment with 12- O-tetradecanoylphorbol-13-acetate (TPA) and to elucidate the underlying molecular mechanism of CS action in mouse skin inflammation. TPA was topically applied to the shaven backs of ICR mice with or without CS (1 or 2 mg) for 4 h. The results demonstrated that CS suppressed TPA-induced edema and reduced the expression of cyclooxygenase-2, vascular cell adhesion molecule-1, and Akt signaling in mouse skin. These studies suggest that CS from ascidian tunic may be developed as an effective natural anti-inflammatory agent.
Xu
Cheng-Xiong
CX
Laboratory of Toxicology, Seoul National University, Seoul 151-742, Korea.
Jin
Hua
H
Chung
Youn-Sun
YS
Shin
Ji-Young
JY
Lee
Kee-Ho
KH
Beck
George R
GR
Palmos
Grace N
GN
Choi
Byeong-Dae
BD
Cho
Myung-Haing
MH
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D002457:Cell Extracts / Q000494:pharmacology; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D010703:Phorbol Esters / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D011897:Random Allocation; D015398:Signal Transduction; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D014561:Urochordata / Q000737:chemistry / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics* / Q000378:metabolism
D002273:Carcinogens; D002457:Cell Extracts; D016328:NF-kappa B; D010703:Phorbol Esters; D019010:Vascular Cell Adhesion Molecule-1; D002809:Chondroitin Sulfates; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2