title: Lyophilized turkey herpesvirus (HVT): stability of vaccine and minimum protective dose.
abstract: The research reported showed that: the stability of cell-free HVT vaccine, lyophilized and sealed under vacuum, is very high: after one year at 4 degrees -6 degrees C and after 10 days at 37 degrees C the titre decreased only about 0.4 log(10)', the stability of the vaccine, when reconstituted for use in the diluent (sucrose-phosphate buffer), is high (80%) for at least 2 hours; the degree of protection, found in 3 tests, was about 90% with an inoculum of 20 to 100 pfu, and the PD50 was 4.9 pfu of virus per chick.
authors.affiliation: Institute of Infectious Diseases, Veterinary Faculty, Italy Research Laboratory, University of Milano, Brescia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Zanella
authors.forename: V
authors.initials: V
authors.lastname: Bettoni
authors.forename: N
authors.initials: N
authors.lastname: Mambelli