Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor beta in HEK293 cells.
Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor beta, (PPARbeta). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARbeta in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARbeta. Furthermore, PPARbeta also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.
Song
Hae Jin
HJ
Department of Biology, College of Life Science and Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul 120-749, Republic of Korea.
Park
Joongkyu
J
Seo
Su Ryeon
SR
Kim
Jongsun
J
Paik
Seung R
SR
Chung
Kwang Chul
KC
D002460:Cell Line; D015723:Gene Library; D006801:Humans; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D048068:PPAR-beta / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
D008565:Membrane Proteins; D018832:Molecular Chaperones; D009124:Muscle Proteins; D048068:PPAR-beta; C410584:PSMG1 protein, human; D011480:Protease Inhibitors