title: [Effects of honokiol on proliferation and apoptosis of human cervical carcinoma cell line Hela in vitro].
abstract: OBJECTIVE
  To assess the effects of honokiol on proliferation and apoptosis of human cervical carcinoma cell line Hela in vitro.
  
  
  METHODS
  Cultured HeLa cells were treated with different concentrations of honokiol for the varieties of period (24, 48, 72, 96 h). Cell proliferation was assessed by MTT colorimetric assay. Cell apoptosis was determined by flow cytometry (FCM), Hoechst 33258 fluorescent staining and DNA ladder respectively.
  
  
  RESULTS
  MTT assay demonstrated that the proliferation of Hela cells were suppressed significantly by honokiol in dose-and time-dependent manner. FCM analysis showed that the apoptosis rates of Hela cells treated with 10 microg/mL and 20 microg/mL honokiol for 24 h were 22.5% and 62.2%, respectively, while that of the control group cells was 8.7%. After treatment with honokiol, typically morphologic changes of apoptosis were observed by Hoechst 33258 fluorescence staining; Genomic DNA from Hela cells treated with honokiol displayed a characteristic ladder pattern on agarose gel electrophoresis.
  
  
  CONCLUSIONS
  honokiol can inhibit the proliferation and induce apoptosis of human cervical carcinoma cell line Hela.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Guang-li
authors.initials: GL
authors.lastname: Yang
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Hou
authors.forename: A-fu
authors.initials: AF
authors.lastname: Fu
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Zhong
authors.forename: Xiu-hua
authors.initials: XH
authors.lastname: Yin
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Chen
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D013997:Time Factors; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D017705:Lignans; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols