Synthesis and incorporation of fluorescent C-nucleoside analogue into oligodeoxyribonucleotides.
Fluorescent nucleoside analogues were synthesized and derived to protected nucleoside phosphoramidite. The nucleoside analogue bearing 3-aminobenzonitrile was sensitive to the surrounding environment. 3-Aminobenzonitrile is a similar size with pyrimidine base. Therefore, it is expected that the nucleoside little perturbs the formation of duplex. This nucleoside could be incorporated into DNA.
Ozaki
Hiroaki
H
Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan. h-ozaki@chem-bio.gunma-u.ac.jp
Kawai
Takeshi
T
Kuwahara
Masayasu
M
Sawai
Hiroaki
H
D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D009705:Nucleosides / Q000138:chemical synthesis / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*
C541625:6-(2-deoxyribofuranosyl)-3-aminobenzonitrile; D000814:Aniline Compounds; D005456:Fluorescent Dyes; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D004247:DNA