title: [Screening of human anti-TNF-alpha scFv from large phage library].
abstract: OBJECTIVE
  To clone human anti-TNF-alpha scFv from large phage antibody library.
  
  
  METHODS
  Panning of large phage library against TNF-alpha was conducted to select specific antibodies. The antigen binding activity and DNA sequence were determined and analyzed by ELISA, restriction enzyme map.
  
  
  RESULTS
  After 4 rounds of panning, 62 positive clones were obtained and 27 clones had specific binding ability to TNF-alpha. DNA fingerprinting of the 27 clones showed 7 different stripes indicated 7 different positive clones. 5 of the 7 clones expressed soluble anti-TNF-alpha activity. DNA sequence analysis showed that the variable regions of these scFvs belonged to different subgroups.
  
  
  CONCLUSIONS
  Human TNF-alpha scFv have been successfully obtained from large phage antibody library.
authors.affiliation: Navy General Hospital, Beijing 100037, China.
authors.forename: Yu-xiao
authors.initials: YX
authors.lastname: Wang
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Qiao
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000906:Antibodies / Q000235:genetics / Q000276:immunology; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology*; D019151:Peptide Library; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology*
chemical_list: D000906:Antibodies; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D014409:Tumor Necrosis Factor-alpha