glo-3, a novel Caenorhabditis elegans gene, is required for lysosome-related organelle biogenesis.
Gut granules are specialized lysosome-related organelles that act as sites of fat storage in Caenorhabditis elegans intestinal cells. We identified mutations in a gene, glo-3, that functions in the formation of embryonic gut granules. Some glo-3(-) alleles displayed a complete loss of embryonic gut granules, while other glo-3(-) alleles had reduced numbers of gut granules. A subset of glo-3 alleles led to mislocalization of gut granule contents into the intestinal lumen, consistent with a defect in intracellular trafficking. glo-3(-) embryos lacking gut granules developed into adults containing gut granules, indicating that glo-3(+) function may be differentially required during development. We find that glo-3(+) acts in parallel with or downstream of the AP-3 complex and the PGP-2 ABC transporter in gut granule biogenesis. glo-3 encodes a predicted membrane-associated protein that lacks obvious sequence homologs outside of nematodes. glo-3 expression initiates in embryonic intestinal precursors and persists almost exclusively in intestinal cells through adulthood. GLO-3GFP localizes to the gut granule membrane, suggesting it could play a direct role in the trafficking events at the gut granule. smg-1(-) suppression of glo-3(-) nonsense alleles indicates that the C-terminal half of GLO-3, predicted to be present in the cytoplasm, is not necessary for gut granule formation. Our studies identify GLO-3 as a novel player in the formation of lysosome-related organelles.
Rabbitts
Beverley M
BM
Department of Biology, Lewis & Clark College, Portland, Oregon 97219, USA.
Ciotti
Marcela K
MK
Miller
Natalie E
NE
Kramer
Maxwell
M
Lawrenson
Andrea L
AL
Levitte
Steven
S
Kremer
Susan
S
Kwan
Elizabeth
E
Weis
Allison M
AM
Hermann
Greg J
GJ
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000166:cytology / Q000378:metabolism; D017238:Genes, Helminth; D008247:Lysosomes / Q000378:metabolism*; D008969:Molecular Sequence Data; D010641:Phenotype
D029742:Caenorhabditis elegans Proteins
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