title: The proton-translocating a subunit of F0F1-ATP synthase is allocated asymmetrically to the peripheral stalk.
abstract: The position of the a subunit of the membrane-integral F0 sector of Escherichia coli ATP synthase was investigated by single molecule fluorescence resonance energy transfer studies utilizing a fusion of enhanced green fluorescent protein to the C terminus of the a subunit and fluorescent labels attached to specific positions of the epsilon or gamma subunits. Three fluorescence resonance energy transfer levels were observed during rotation driven by ATP hydrolysis corresponding to the three resting positions of the rotor subunits, gamma or epsilon, relative to the a subunit of the stator. Comparison of these positions of the rotor sites with those previously determined relative to the b subunit dimer indicates the position of a as adjacent to the b dimer on its counterclockwise side when the enzyme is viewed from the cytoplasm. This relationship provides stability to the membrane interface between a and b2, allowing it to withstand the torque imparted by the rotor during ATP synthesis as well as ATP hydrolysis.
authors.affiliation: 3, Physikalisches Institut, Universität Stuttgart, 70550 Stuttgart, Germany.
authors.forename: Monika G
authors.initials: MG
authors.lastname: Düser
authors.forename: Yumin
authors.initials: Y
authors.lastname: Bi
authors.forename: Nawid
authors.initials: N
authors.lastname: Zarrabi
authors.forename: Stanley D
authors.initials: SD
authors.lastname: Dunn
authors.forename: Michael
authors.initials: M
authors.lastname: Börsch
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D025243:Bacterial Proton-Translocating ATPases / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D006868:Hydrolysis; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000378:metabolism; D020836:Protein Structure, Quaternary / Q000502:physiology; D021122:Protein Subunits / Q000737:chemistry* / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; D021122:Protein Subunits; D000255:Adenosine Triphosphate; D025243:Bacterial Proton-Translocating ATPases
references.citation: Angew Chem Int Ed Engl. 2007;46(7):1172-5
references.pmid: 17211912
references.citation: FEBS Lett. 1998 Apr 17;426(2):217-20
references.pmid: 9599011
references.citation: J Phys Chem B. 2006 Apr 6;110(13):6970-8
references.pmid: 16571010
references.citation: Biochemistry. 2002 May 28;41(21):6875-84
references.pmid: 12022893
references.citation: Angew Chem Int Ed Engl. 2008;47(5):974-6
references.pmid: 18098261
references.citation: J Biol Chem. 1998 Jun 26;273(26):16241-7
references.pmid: 9632683
references.citation: Nature. 2001 Apr 19;410(6831):898-904
references.pmid: 11309608
references.citation: J Biol Chem. 2003 Jul 18;278(29):27068-71
references.pmid: 12724321
references.citation: Chemphyschem. 2006 Jan 16;7(1):250-60
references.pmid: 16353266
references.citation: Biochim Biophys Acta. 2006 May-Jun;1757(5-6):311-9
references.pmid: 16765907
references.citation: Biochim Biophys Acta. 1999 Jun 30;1412(2):118-28
references.pmid: 10393255
references.citation: J Biol Chem. 1992 Oct 25;267(30):21355-9
references.pmid: 1400447
references.citation: Biochemistry. 2008 Mar 18;47(11):3564-75
references.pmid: 18298081
references.citation: Proc Natl Acad Sci U S A. 1967 Aug;58(2):719-26
references.pmid: 5233469
references.citation: EMBO J. 2003 Dec 1;22(23):6182-92
references.pmid: 14633978
references.citation: Biochim Biophys Acta. 1997 Mar 28;1319(1):19-58
references.pmid: 9107315
references.citation: FEBS Lett. 2002 Sep 11;527(1-3):147-52
references.pmid: 12220651
references.citation: Annu Rev Biochem. 1997;66:717-49
references.pmid: 9242922
references.citation: Science. 1996 Sep 6;273(5280):1392-5
references.pmid: 8703075
references.citation: FEBS Lett. 1999 Sep 3;457(3):327-32
references.pmid: 10471802
references.citation: Biochemistry. 1989 May 30;28(11):4709-16
references.pmid: 2475169
references.citation: J Biol Chem. 1987 Mar 5;262(7):3060-4
references.pmid: 2880846
references.citation: Annu Rev Phys Chem. 2001;52:233-53
references.pmid: 11326065
references.citation: J Biol Chem. 2000 Jun 9;275(23):17571-7
references.pmid: 10747904
references.citation: J Biol Chem. 2006 Feb 17;281(7):4126-31
references.pmid: 16352612
references.citation: Biophys J. 2006 Sep 1;91(5):1941-51
references.pmid: 16766620
references.citation: Nat Struct Mol Biol. 2004 Feb;11(2):135-41
references.pmid: 14730350
references.citation: J Biol Chem. 2005 Sep 30;280(39):33338-45
references.pmid: 16085645
references.citation: J Am Chem Soc. 2008 Apr 23;130(16):5398-9
references.pmid: 18376823
references.citation: J Biol Chem. 1999 May 28;274(22):15598-604
references.pmid: 10336456
references.citation: EMBO J. 2006 Jun 21;25(12):2911-8
references.pmid: 16791136
references.citation: J Biol Chem. 1986 Aug 5;261(22):10037-42
references.pmid: 2874136
references.citation: Annu Rev Biochem. 1998;67:509-44
references.pmid: 9759496
references.citation: Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):135-40
references.pmid: 18162559
references.citation: Science. 2005 Apr 29;308(5722):659-62
references.pmid: 15860619
references.citation: J Mol Biol. 2002 Aug 23;321(4):613-9
references.pmid: 12206777
references.citation: Nat Struct Biol. 2000 Nov;7(11):1051-4
references.pmid: 11062562
references.citation: J Biol Chem. 2003 May 16;278(20):18544-9
references.pmid: 12624095
references.citation: J Exp Biol. 2000 Jan;203(Pt 1):9-17
references.pmid: 10600668
references.citation: Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13179-83
references.pmid: 14595019
references.citation: Structure. 2006 Apr;14(4):633-43
references.pmid: 16615904
references.citation: J Biol Chem. 1997 Aug 1;272(31):19621-4
references.pmid: 9235970
references.citation: J Mol Biol. 2000 Feb 18;296(2):449-57
references.pmid: 10669600
references.citation: Biochim Biophys Acta. 2008 Feb;1777(2):227-37
references.pmid: 18178144
references.citation: J Histochem Cytochem. 1999 Sep;47(9):1179-88
references.pmid: 10449539
references.citation: EMBO J. 2005 Jun 15;24(12):2053-63
references.pmid: 15920483