The association of membrane frizzled-related protein (MFRP) gene with acute angle-closure glaucoma--a pilot study.
OBJECTIVE
The membrane frizzled-related protein (MFRP) has been proposed as a probable candidate gene for extreme hyperopia and nanophthalmos, which are factors for angle-closure glaucoma. The purpose of our study was to investigate whether there are significant associations between angle-closure glaucoma and sequence variants in the MFRP gene reported previously in Taiwanese subjects.


METHODS
Genomic DNA was collected from 63 subjects with angle-closure glaucoma and 66 age-matched and gender-matched controls without angle-closure glaucoma. Three sequence variants were detected by polymerase chain reaction (PCR) and direct sequencing in all of the cases and controls.


RESULTS
None of the three sequence variants showed a significant result in terms of association with disease. The pairwise linkage disequilibrium (LD) mapping confirmed that these alleles have a comparatively strong LD index greater than 0.7 for D' and greater than 0.4 for r(2) at these polymorphisms. However, we found there were no statistical associations between any of the three sequence variants located on MFRP and angle-closure glaucoma.


CONCLUSIONS
In our pilot study, variations that we tested in MFRP were not associated with the development of acute angle-closure glaucoma in Taiwanese subjects.
Wang
I-Jong
IJ
Department of Ophthalmology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
Lin
Shan
S
Chiang
Ting-Hsuan
TH
Chen
Zoe Tzu-Yi
ZT
Lin
Luke L K
LL
Hung
Por-Tying
PT
Shih
Yung-Feng
YF
D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D015812:Glaucoma, Angle-Closure / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D000099274:Frizzled-Related Proteins
D008565:Membrane Proteins; C490110:MFRP protein, human; D000099274:Frizzled-Related Proteins
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