Clinical and histological responses to laparoscopically-induced peritonitis in rats.
OBJECTIVE
To evaluate the efficacy of inducing peritonitis in rats through laparoscopic cecal ligation (CL), by means of an elastic band.


METHODS
Twelve Wistar rats were subjected to cecal ligated with an elastic band applied using a specially constructed applicator. In six of the animals (the CL group) the cecal sac was preserved intact whilst in the remaining animals (the CLP group) the sac was perforated with scissors. Clinical parameters, characteristics of the peritoneal cavity and cecum, and histological features of the cecal tissue were observed in all experimental animals 8 and 24 h after surgery.


RESULTS
CLP animals exhibited at least one clinical sign of sepsis within the first 8 h of observation. The peritoneal liquid was observed to be clear in almost all members of the CLP. Polymorphonucleated cells were detected in the tunica serosa of the cecum of CLP animals. In contrast, all members of the CL group were alive after 24h, and of polymorphonucleated cells in the muscle layer of the cecal wall were observed. The presence of peritoneal liquid was not detected in CL animals.


CONCLUSIONS
Although elastic ligation of the cecum was reproducible, puncture of the cecal sac was essential for induction of sepsis.
Bicalho
Paulo Roberto Rodrigues
PR
School of Medicine, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Lima
Leandro Bueno
LB
Alvarenga
Daniel Gomes de
DG
Duval-Araujo
Ivana
I
Nunes
Tarcizo Afonso
TA
Dos Reis
Fabíola Alves
FA
D000818:Animals; D002432:Cecum / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D005260:Female; D010535:Laparoscopy / Q000379:methods*; D008026:Ligation; D010538:Peritonitis / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000473:pathology*; D013997:Time Factors