title: Isolation of human peripheral blood mononuclear cells (PBMCs).
abstract: In this appendix, several basic methods are described for preparation of primary B lymphocyte.
authors.affiliation: University of Pennsylvania Medical School, Philadelphia, Pennsylvania, USA.
authors.forename: Ke
authors.initials: K
authors.lastname: Lan
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Verma
authors.forename: Masanao
authors.initials: M
authors.lastname: Murakami
authors.forename: Bharat
authors.initials: B
authors.lastname: Bajaj
authors.forename: Erle S
authors.initials: ES
authors.lastname: Robertson
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology; D002499:Centrifugation, Density Gradient / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000166:cytology*