[Correlation analysis between single nucleotide polymorphism of FGF5 gene and wool yield in rabbits].
Single nucleotide polymorphism (SNP) in exon 1 and 3 of fibroblast growth factor (FGF5) gene was studied by DNA sequencing in Yingjing angora rabbit, Tianfu black rabbit and California rabbit. A frameshift mutation (TCT insert) at base position 217 (site A) of exon 1 and a T/C missense mutation at base position 59 (site B) of exon 3 were found in Yingjing angora rabbit with a high frequency; a T/C same-sense mutation at base position 3 (site C) of exon 3 was found with similar frequency in three rabbit breeds. Least square analysis showed that different genotypes had no significant association with wool yield in site A, and had high significant association with wool yield in site B (P<0.01) and significant association with wool yield in site C (P<0.05). It was concluded from the results that FGF5 gene could be the potential major gene affecting wool yield or link with the major gene, and polymorphic loci B and C may be used as molecular markers for im-proving wool yield in angora rabbits.
Li
Chun-Xiao
CX
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China. lcx20041979@163.com
Jiang
Mei-Shan
MS
Chen
Shi-Yi
SY
Lai
Song-Jia
SJ
D000818:Animals; D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D051521:Fibroblast Growth Factor 5 / Q000235:genetics*; D016368:Frameshift Mutation / Q000235:genetics; D005838:Genotype; D020125:Mutation, Missense / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011817:Rabbits; D014935:Wool / Q000378:metabolism*
D051521:Fibroblast Growth Factor 5