title: Interleukin-20 targets renal mesangial cells and is associated with lupus nephritis.
abstract: Lupus nephritis is one manifestation of systemic lupus erythematosus (SLE). Interleukin (IL)-10 is involved in the pathogenesis of SLE. To determine whether IL-20, a member of the IL-10 family, is associated with lupus nephritis, we analyzed the expression of IL-20 and its receptors in mesangial cells derived from SLE-prone, NZB/W, and DBA/W mice. IL-20 and its receptors were upregulated in mesangial cells from NZB/W mice. Incubating IL-20 with mesangial cells upregulated the transcripts of CCL2 (MCP-1), CCL5 (RANTES), CXCL10 (IP-10), IL-6, iNOS, and ROS, all of which are involved in the pathogenesis of lupus nephritis. IL-20 specifically activated the downstream signal ERK 1/2. We also detected human IL-20 protein in both mesangial cells and inflammatory cells in kidney biopsies of patients with lupus nephritis. Our results reveal the novel effects of IL-20 on mesangial cells and its association with lupus nephritis.
authors.affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Hsing-Hui
authors.initials: HH
authors.lastname: Li
authors.forename: He-Hsiung
authors.initials: HH
authors.lastname: Cheng
authors.forename: Kuang-Hui
authors.initials: KH
authors.lastname: Sun
authors.forename: Chi-Chen
authors.initials: CC
authors.lastname: Wei
authors.forename: Chien-Feng
authors.initials: CF
authors.lastname: Li
authors.forename: Wen-Chung
authors.initials: WC
authors.lastname: Chen
authors.forename: Wen-Mein
authors.initials: WM
authors.lastname: Wu
authors.forename: Ming-Shi
authors.initials: MS
authors.lastname: Chang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis; D002648:Child; D016207:Cytokines / Q000096:biosynthesis; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000502:physiology*; D008181:Lupus Nephritis / Q000209:etiology*; D008297:Male; D050527:Mesangial Cells / Q000378:metabolism*; D051379:Mice; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D017382:Reactive Oxygen Species; D013154:Spleen / Q000276:immunology
chemical_list: D018925:Chemokines; D016207:Cytokines; D007378:Interleukins; D017382:Reactive Oxygen Species; D052247:Nitric Oxide Synthase Type II; D048049:Extracellular Signal-Regulated MAP Kinases; C422042:interleukin 20