[Structural homology between streptolysin O (SLO) produced by streptococcus pyogenes and SLO-like protein produced by non-pathogenic streptococci and cross-reactivity of antibody against SLO-like protein to SLO].
Nine clones of non-pathogenic streptococci were isolated from the pharynges of seven healthy subjects, and grown on sheep blood agar plates with a hemolysis or gamma hemolysis, then cultured in LB broth for 16 hrs. Purified streptolysin O (SLO) purchased from Sigma Chemical Co. (Sigma-SLO), SLO antigen as a latex agglutination reagent from A company (A-SLO) and supernatants from four culture media were electrophoresed on 12% SDS-polyacrylamide gel and transferred to PVDF membranes. Immunological analyses of antibodies against SLO in healthy sera and proteins in culture medium demonstrated that healthy sera contained an antibody recognizing Sigma-SLO, A-SLO and a protein of the same size as SLO (SLO-like protein) in culture medium. These findings suggest that healthy subjects develop an antibody directed against SLO-like protein produced by non-pathogenic streptococci, and that this antibody cross-reacts with Sigma-SLO and A-SLO. Using DNA from Streptococcus pyogenes and non-pathogenic streptococci, the SLO gene and SLO-like protein gene were analyzed by direct sequencing with oligonucleotide primers designed to cover no. 74 to approximately 1900 of the SLO gene. There were three different bases resulting in amino acid substitution between the SLO gene and SLO-like protein gene, namely 101Lys (AAA) of SLO to Asn (AAT), 175Met (ATG) to Arg (AGG) and 185Asp (GAT) to Asn (AAT). Remaining 560 residues of 563 amino acids constituting SLO-like protein were homologous to SLO. Non-pathogenic streptococci on the pharynges of healthy subjects produce an SLO-like protein composed of amino acids similar to those of SLO, which induces an antibody against this SLO-like protein in serum. It is likely that an antibody against SLO-like protein in healthy sera cross-reacts with SLO and causes a pseudo-positive reaction on ASO measurement by the latex agglutination method using SLO antigen.
Iijima
Kenji
K
Department of Pathobiological Science and Technology, School of Health Science, Faculty of Medicine, Tottori University Yonago 683-8503.
Koike
Hisashi
H
Ota
Hiromi
H
Nakagawa
Mayumi
M
Nishikawa
Ken-Ichi
K
Kotani
Kazuhiko
K
D000328:Adult; D000906:Antibodies / Q000276:immunology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D010614:Pharynx / Q000382:microbiology; D013291:Streptococcus / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000378:metabolism*; D013301:Streptolysins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*
D000906:Antibodies; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D013301:Streptolysins; C025964:streptolysin O