Involvement of glyceraldehyde-3-phosphate dehydrogenase in the X-Ray resistance of HeLa cells.
We investigated changes in the sub-cellular distribution of glycelaldehyde-3-phosphate dehydrogenase (GAPDH) after X-ray irradiation in HeLa cells. Twenty-four h after irradiation at 5 Gy, nuclear GAPDH levels increased 2.6-fold, whereas total GAPDH levels increased only 1.2-fold. Knockdown of GAPDH using specific small interfering RNA (siRNA) led to sensitization to X-ray-induced cell death. These results suggest that GAPDH plays a role in the radioresponse.
Lu
Jun
J
Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, Chiba, Japan.
Suzuki
Toshikazu
T
Lu
Sa
S
Suzuki
Nobuo
N
D002470:Cell Survival / Q000528:radiation effects; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D034741:RNA, Small Interfering / Q000494:pharmacology; D011836:Radiation Tolerance / Q000187:drug effects / Q000235:genetics*; D014162:Transfection; D014965:X-Rays
D034741:RNA, Small Interfering; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases