Heterogeneous nuclear ribonuclear protein K interacts with the enterovirus 71 5' untranslated region and participates in virus replication.
Enterovirus 71 (EV71) is a picornavirus that can cause severe neurological complications in children. Like other picornaviruses, the genomic RNA of EV71 contains a long 5' untranslated region (UTR). Cellular proteins interact with the EV71 5' UTR, and these interactions are important for virus replication. Using an RNA pull-down assay and proteomics approaches, this study identified the heterogeneous nuclear ribonucleoprotein K (hnRNP K) as one of the EV71 5' UTR-associated proteins. The interaction between hnRNP K and the 5' UTR was further confirmed by mapping the interaction regions to stem-loops I-II and IV in the 5' UTR. During EV71 infection, hnRNP K was enriched in the cytoplasm where virus replication occurs, whereas hnRNP K was localized in the nucleus in mock-infected cells. Viral yields were found to be significantly lower in hnRNP K knockdown cells and viral RNA synthesis was delayed in hnRNP K knockdown cells in comparison with negative-control cells treated with small interfering RNA. These results suggest that hnRNP K interacts with the EV71 5' UTR and participates in virus replication.
Lin
Jing-Yi
JY
Graduate Program in Biomedical Science, Chang Gung University, Taoyuan, Taiwan, ROC.|Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan, Taiwan, ROC.|Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan, ROC.
Li
Mei-Ling
ML
Department of Molecular Genetics, Microbiology and Immunology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ, USA.
Huang
Peng-Nien
PN
Graduate Program in Biomedical Science, Chang Gung University, Taoyuan, Taiwan, ROC.|Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan, Taiwan, ROC.|Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan, ROC.
Chien
Kun-Yi
KY
Graduate Program in Biomedical Science, Chang Gung University, Taoyuan, Taiwan, ROC.
Horng
Jim-Tong
JT
Graduate Program in Biomedical Science, Chang Gung University, Taoyuan, Taiwan, ROC.|Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan, Taiwan, ROC.
Shih
Shin-Ru
SR
Graduate Program in Biomedical Science, Chang Gung University, Taoyuan, Taiwan, ROC.|Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan, Taiwan, ROC.|Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan, ROC.
D020121:5' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology; D004770:Enterovirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014779:Virus Replication
D020121:5' Untranslated Regions; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D012367:RNA, Viral