title: Measurements of in vivo intra-articular gentamicin levels from antibiotic loaded articulating spacers in revision total knee replacement.
abstract: Previous in vitro studies have found high levels of antibiotic release in the days immediately following implantation of antibiotic loaded articulating spacers. However there are relatively few data describing the elution profile beyond this immediate period. This study was designed to measure if gentamicin levels continue to be clinically therapeutic after an extended period following in vivo implantation. Twelve patients received a gentamicin loaded articulating spacer between a 1st and 2nd stage revision total knee arthroplasty. At the 2nd stage procedure synovial fluid and blood samples were collected and assayed for the presence of gentamicin. The second stage revision occurred at a median of 99 days following spacer insertion. The median intra-articular gentamicin levels were 0.46 mg/L (0.24 to 2.36 mg/L) which would be considered therapeutic. There were no cases of reinfection. In this study, preformed articulating spacers containing gentamicin provided therapeutic concentrations in the synovial fluid surrounding the joint throughout the period of implantation. These data confirm the observations from in vitro studies, where a prolonged elution profile was observed for such spacers.
authors.affiliation: Trauma and Orthopaedics, Southmead Hospital, Bristol, United Kingdom. jonmutimer@doctors.org.uk
authors.forename: J
authors.initials: J
authors.lastname: Mutimer
authors.forename: G
authors.initials: G
authors.lastname: Gillespie
authors.forename: A M
authors.initials: AM
authors.lastname: Lovering
authors.forename: A J
authors.initials: AJ
authors.lastname: Porteous
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D019645:Arthroplasty, Replacement, Knee / Q000009:adverse effects* / Q000379:methods; D004337:Drug Carriers; D005260:Female; D005839:Gentamicins / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D011446:Prospective Studies; D016459:Prosthesis-Related Infections / Q000188:drug therapy* / Q000601:surgery; D012086:Reoperation
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D005839:Gentamicins