title: A new storage medium for an avulsed tooth.
abstract: OBJECTIVE
  The purpose of this study is to determine the efficacy of egg white in maintaining the viability of human periodontal ligament (PDL) cells on avulsed teeth.
  
  
  METHODS
  The experimental media were: egg white, milk, Hanks' Balanced Salt Solution (HBSS) as the positive control, and tap water as the negative control. The storage times were 1, 2, 4, 8, and 12 hours. Extracted premolar teeth of healthy individuals were rinsed in the media. After trypsinization and subsequent treatment in collagenase, cell viability was determined using trypan blue staining. The two-way analysis of variance (ANOVA) statistical test was used to compare the results among different media.
  
  
  RESULTS
  There was no difference in the cell viability between egg white and HBSS media, but there was a statistically significant difference between the viability of PDL cells in egg white medium in comparison with milk (P<0.05) and water (P<0.05).
  
  
  CONCLUSIONS
  Egg white could be suggested as a suitable storage medium. Its principle advantage is its availability.
authors.affiliation: Department of Endodontics, Isfahan University, Iran.
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Khademi
authors.forename: Saeed
authors.initials: S
authors.lastname: Saei
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Mohajeri
authors.forename: Nooshin
authors.initials: N
authors.lastname: Mirkheshti
authors.forename: Fatima
authors.initials: F
authors.lastname: Ghassami
authors.forename: Nakisa
authors.initials: N
authors.lastname: Torabi nia
authors.forename: Sayyed Ali
authors.initials: SA
authors.lastname: Alavi
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival; D004529:Egg White; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007552:Isotonic Solutions; D008892:Milk; D019188:Organ Preservation Solutions; D009994:Osmolar Concentration; D010513:Periodontal Ligament / Q000166:cytology / Q000502:physiology*; D014084:Tooth Avulsion / Q000628:therapy*
chemical_list: C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions