title: Next-generation sequencing: applications beyond genomes.
abstract: The development of DNA sequencing more than 30 years ago has profoundly impacted biological research. In the last couple of years, remarkable technological innovations have emerged that allow the direct and cost-effective sequencing of complex samples at unprecedented scale and speed. These next-generation technologies make it feasible to sequence not only static genomes, but also entire transcriptomes expressed under different conditions. These and other powerful applications of next-generation sequencing are rapidly revolutionizing the way genomic studies are carried out. Below, we provide a snapshot of these exciting new approaches to understanding the properties and functions of genomes. Given that sequencing-based assays may increasingly supersede microarray-based assays, we also compare and contrast data obtained from these distinct approaches.
authors.affiliation: Cancer Research UK, Fission Yeast Functional Genomics Group, The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK. jurg@sanger.ac.uk
authors.forename: Samuel
authors.initials: S
authors.lastname: Marguerat
authors.forename: Brian T
authors.initials: BT
authors.lastname: Wilhelm
authors.forename: Jürg
authors.initials: J
authors.lastname: Bähler
mesh_terms: D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D016678:Genome; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins
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