[Development of a molecular beacon real-time PCR for the rapid detection of Mycobacterium tuberculosis].
OBJECTIVE
To develop a molecular beacon real-time PCR for rapid detection of Mycobacterium tuberculosis.


METHODS
One set of primers was selected from the IS6110 gene in GenBank and the corresponding molecular beacon probe was designed. The specificity and sensitivity of the developed method were evaluated by tested with 10 different bacteria species. The developed assay were also applied to the diagnosis of tuberculosis.


RESULTS
Only Mycobacterium tuberculosis strains possessing IS6110 gene generated fluorescent signals, and no cross reaction was observed with other 9 bacteria. The detection limit was 4 copies/PCR reaction. 100 Mycobacterium tuberculosis strains were positive tested by Real-time PCR.


CONCLUSIONS
The established molecular beacon real-time PCR is a rapid, specific and sensitive method, and is a beneficial supplement of traditional methods for the tuberculosis diagnosis.
Xu
Ya-jun
YJ
Department of Medical Technology, West China School of Public Health, West China Medical Center, Sichuan University, Chengdu 610041, China.
Zhou
Zi-ren
ZR
Lin
Lin
L
Liu
Qu
Q
Liu
Heng-chuan
HC
D015342:DNA Probes / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D015342:DNA Probes