Biomarkers of oxidative stress and endothelial dysfunction in glucose intolerance and diabetes mellitus.
OBJECTIVE
To evaluate biomarkers of endothelial dysfunction and oxidative stress in glucose intolerance (GI) compared to overt diabetes (DM2).


METHODS
140 volunteers including 96 with DM2, 32 with GI and 12 controls (C) were studied. (*)NO metabolites, (*)NO synthase inhibitors, thiols and N-acetyl-beta-glucosaminidase (NAGase) activity were analyzed by chemiluminescence, capillary electrophoresis, ELISA and colorimetric assay, respectively.


RESULTS
(*)NO metabolites were higher in GI (NOx: p=0.03; S-nitrosothiols: p=0.001) and DM2 (p=0.006; p=0.0006) groups in relation to group C, while nitrotyrosine was higher only in the DM2 group in comparison to the other groups. NAGase activity was elevated in GI (p=0.003) and DM2 (p=0.0004) groups in relation to group C, as well as, ADMA (p=0.01; p=0.003) and GSSG (p=0.01; p=0.002).


CONCLUSIONS
(*)NO metabolites, (*)NO synthase inhibitors, thiols and NAGase are biomarkers suitable to indicate endothelial dysfunction and oxidative stress in the early stages of impaired response to insulin.
Pereira
Edimar Cristiano
EC
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.
Ferderbar
Simone
S
Bertolami
Marcelo Chiara
MC
Faludi
André Arpad
AA
Monte
Osmar
O
Xavier
Hermes Toros
HT
Pereira
Tiago Veiga
TV
Abdalla
Dulcineia Saes Parra
DS
D001120:Arginine / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry; D015415:Biomarkers / Q000097:blood*; D015992:Body Mass Index; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000503:physiopathology; D004727:Endothelium / Q000378:metabolism*; D005260:Female; D018149:Glucose Intolerance / Q000378:metabolism* / Q000503:physiopathology; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress
D015415:Biomarkers; D008055:Lipids; D009566:Nitrates; D009573:Nitrites; C487735:dimethylarginine; D009569:Nitric Oxide; D001120:Arginine; D006596:Hexosaminidases; D005978:Glutathione