title: Integrated analysis of copy number alterations and loss of heterozygosity in human pancreatic cancer using a high-resolution, single nucleotide polymorphism array.
abstract: OBJECTIVE
  To chart molecular genetic events in pancreatic cancer.
  
  
  METHODS
  We analyzed genome-wide copy number alterations and loss of heterozygosity (LOH) in 25 established pancreatic cancer cell lines using a high-density single nucleotide polymorphism (SNP) array. We verified the data using genomic PCR and applied them to clinical samples.
  
  
  RESULTS
  Twenty-six homozygous deletion regions were detected in at least 1 cell line and LOH was found at 9p, 18q, 17p, 8p, 13q, 6q, 3p, 6p, 22q, 9q and 12q with high frequency (>50%), consistent with a previous study. Moreover, we found 23 amplified regions in at least 2 cell lines, including 8 unreported loci. We then examined representative genes at the 8 amplified loci in matched pairs of pancreatic cancer and normal tissues. The amplification was detected in 1 (7.1%) to 5 (35.7%) of 14 microdissected tissue specimens.
  
  
  CONCLUSIONS
  Using high-resolution SNP arrays, we studied genome-wide copy number alterations and LOH simultaneously. We identified several novel and minute genomic amplifications, which contained candidate oncogenes in human pancreatic cancers.
authors.affiliation: Department of Gastroenterology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Lian-Jie
authors.initials: LJ
authors.lastname: Lin
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Asaoka
authors.forename: Motohisa
authors.initials: M
authors.lastname: Tada
authors.forename: Masashi
authors.initials: M
authors.lastname: Sanada
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Nannya
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tateishi
authors.forename: Miki
authors.initials: M
authors.lastname: Ohta
authors.forename: Motoko
authors.initials: M
authors.lastname: Seto
authors.forename: Naoki
authors.initials: N
authors.lastname: Sasahira
authors.forename: Minoru
authors.initials: M
authors.lastname: Tada
authors.forename: Takao
authors.initials: T
authors.lastname: Kawabe
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Zheng
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kanai
authors.forename: Seishi
authors.initials: S
authors.lastname: Ogawa
authors.forename: Masao
authors.initials: M
authors.lastname: Omata
mesh_terms: D045744:Cell Line, Tumor; D018628:Gene Dosage; D006801:Humans; D019656:Loss of Heterozygosity; D010190:Pancreatic Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide