Activation of MAP kinase (ERK1/2) in human neonatal colonic enteric nervous system.
The aim of this study was to examine mitogen-activated protein kinase (ERK1/2) activation in the human neonatal colonic enteric nervous system. For this, we investigated by immunocytochemistry the cellular localization of phosphorylated ERK1/2 (P-ERK) in a series of normal human colon samples removed from newborns and in patients with intestinal obstruction such as Hirschsprung's disease (HSCR), stenosis and atresia. We checked the presence of P-ERK in the three distinct histological layers of normal colon. Phosphorylated ERK was detected in the colonic mucosa, in the enteric nervous system and in endothelial cells. In the mucosa from normal colon, P-ERK was detected at the upper part of the crypt, while P-ERK activation in epithelial cells is altered in HSCR, stenosis and atresia. In the normal colon, strong P-ERK staining was detected in myenteric and submucosal enteric plexuses. Using confocal microscopy analyses, we observed that P-ERK staining was localized in enteric glial cells and not in enteric neurons. Strong P-ERK staining was also observed in plexuses from stenosis and atresia whereas in HSCR, hypertrophic nerve fibres were not stained.
Rouleau
C
C
INSERM, ERI 25, Muscle and Pathologies, Montpellier, France.
Matécki
S
S
Kalfa
N
N
Costes
V
V
de Santa Barbara
P
P
D003106:Colon / Q000033:anatomy & histology / Q000201:enzymology* / Q000294:innervation*; D017615:Enteric Nervous System / Q000033:anatomy & histology / Q000201:enzymology*; D004789:Enzyme Activation; D006627:Hirschsprung Disease / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007409:Intestinal Atresia / Q000201:enzymology / Q000473:pathology; D007415:Intestinal Obstruction / Q000201:enzymology / Q000473:pathology; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D010766:Phosphorylation
D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3
J Mol Diagn. 2006 May;8(2):157-69
16645201
Nat Rev Neurosci. 2004 Mar;5(3):173-83
14976517
Neurosurgery. 2006 Apr;58(4):740-8; discussion 740-8
16575338
J Biol Chem. 2007 Oct 5;282(40):29621-33
17675293
Cell. 2000 Oct 13;103(2):211-25
11057895
Am J Med Genet. 2002 Dec 30;115(4):221-30
12503117
Mol Neurobiol. 1998 Feb;16(1):79-95
9554703
Acta Neuropathol. 1988;76(2):159-65
3407393
Cell Tissue Res. 2005 Mar;319(3):367-82
15672264
Cell Tissue Res. 1981;214(3):529-40
7214465
Histochem Cell Biol. 2002 Mar;117(3):235-41
11914921
Dev Cell. 2006 Sep;11(3):339-48
16950125
Am J Physiol Gastrointest Liver Physiol. 2004 May;286(5):G736-46
14701721
EMBO Rep. 2006 Aug;7(8):782-6
16880823
Development. 2003 Oct;130(19):4527-37
12925581
J Med Genet. 2001 Nov;38(11):729-39
11694544