Effect of metal binding and posttranslational lysine carboxylation on the activity of recombinant hydantoinase.
Bacterial hydantoinase possesses a binuclear metal center in which two metal ions are bridged by a posttranslationally carboxylated lysine. How the carboxylated lysine and metal binding affect the activity of hydantoinase was investigated. A significant amount of iron was always found in Agrobacterium radiobacter hydantoinase purified from unsupplemented cobalt-, manganese-, or zinc-amended Escherichia coli cell cultures. A titration curve for the reactivation of apohydantoinase with cobalt indicates that the first metal was preferentially bound but did not give any enzyme activity until the second metal was also attached to the hydantoinase. The pH profiles of the metal-reconstituted hydantoinase were dependent on the specific metal ion bound to the active site, indicating a direct involvement of metal in catalysis. Mutation of the metal binding site residues, H57A, H59A, K148A, H181A, H237A, and D313A, completely abolished hydantoinase activity but preserved about half of the metal content, except for K148A, which lost both metals in its active site. However, the activity of K148A could be chemically rescued by short-chain carboxylic acids in the presence of cobalt, indicating that the carboxylated lysine was needed to coordinate the binuclear ion within the active site of hydantoinase. The mutant D313E enzyme was also active but resulted in a pH profile different from that of wild-type hydantoinase. A mechanism for hydantoinase involving metal, carboxylated K148, and D313 was proposed.
Huang
Cheng-Yang
CY
Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
Hsu
Ching-Chen
CC
Chen
Mei-Chun
MC
Yang
Yuh-Shyong
YS
D000581:Amidohydrolases / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002264:Carboxylic Acids / Q000737:chemistry; D002384:Catalysis; D003035:Cobalt / Q000378:metabolism*; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012231:Rhizobium / Q000201:enzymology*; D013329:Structure-Activity Relationship; D015032:Zinc / Q000378:metabolism*
D002264:Carboxylic Acids; D011994:Recombinant Proteins; D003035:Cobalt; D000581:Amidohydrolases; C020047:dihydropyrimidinase; D015032:Zinc; D008239:Lysine
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