Extracellular matrix gene expression after vocal fold injury in a rabbit model.
OBJECTIVE
We determined the expression of matrix metalloproteinase (MMP)-1, MMP-9, collagen types I and III, and fibronectin from rabbit vocal folds after injury.


METHODS
Thirty rabbits were involved in this study. Five animals were assigned to each time period. Noninjured vocal fold specimens were collected as a control. Gene expression was analyzed at 2, 4, 8, 24, 48, and 72 hours after injury by use of real-time polymerase chain reaction.


RESULTS
Compared to 2 hours after injury, MMP-1 expression was increased at 4, 8, 24, 48, and 72 hours. Compared to 4 hours, MMP-1 expression was increased at 8, 24, 48, and 72 hours. Compared to the control specimens, MMP-9 expression was increased at 4, 8, 24, 48, and 72 hours. Compared to 2 hours, MMP-9 expression was increased at 4, 8, 24, 48, and 72 hours. Compared to 2 and 4 hours, collagen type I expression was increased at 72 hours. Collagen type III expression was increased at 72 hours compared to 2, 4, and 8 hours. Compared to 2 hours, fibronectin expression was increased at 24, 48, and 72 hours.


CONCLUSIONS
Results revealed time-dependent changes in expression of MMP-1, MMP-9, collagen types I and III, and fibronectin from rabbit vocal folds after injury. Future experiments are planned to investigate the effects of phonation on expression of these genes after injury.
Rousseau
Bernard
B
Department of Otolaryngology, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, Nashville, Tennessee 37232-4480, USA.
Ge
Ping Jiang
PJ
Ohno
Tsunehisa
T
French
Lesley C
LC
Thibeault
Susan L
SL
D000818:Animals; D002921:Cicatrix / Q000150:complications; D017931:DNA Primers / Q000235:genetics; D005109:Extracellular Matrix / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014826:Vocal Cord Paralysis / Q000150:complications / Q000209:etiology* / Q000235:genetics*; D014827:Vocal Cords / Q000293:injuries*; D014832:Voice Disorders / Q000209:etiology; D014947:Wounds and Injuries / Q000150:complications
D017931:DNA Primers; D012333:RNA, Messenger; D020781:Matrix Metalloproteinase 1
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