title: [cDNA cloning and protein structure analysis of growth hormone from Clarias lazera].
abstract: Total RNA was isolated from pituitary gland of Clarias lazera, and the cDNA encoding growth hormone (GH) protein was amplified and cloned by reverse transcription polymerase chain reaction (RT-PCR). The open reading frame (ORF) of cDNA is of 603 nt which encodes GH precursor consisted of a signal peptide with 22 amino acid residues and a mature peptide with 178 amino acid residues. Sequence alignment indicated that the amino acid sequence homology approached to 95.8% between C. lazera and other 6 species of Siluriforms catfish. Secondary structure assessment showed that the GH protein contained different structural regions of alpha-helix, beta-sheet, beta-turn and random coil, among which alpha-helix has main proportion. Antigenicity analysis indicates that there exist 4 domains in amino acid sequence where B cell dominant epitopes could form. Summarily, the structure characteristics of C. lazera GH should provide a great benefit in its modification into recombinant vaccine or monoclonal antibody for future application.
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning 530005, China. nnyxm@sina.com
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Yang
authors.forename: Jing-Zhou
authors.initials: JZ
authors.lastname: He
authors.forename: Xiong-Jun
authors.initials: XJ
authors.lastname: Huang
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Li
authors.forename: Ya-Fen
authors.initials: YF
authors.lastname: Guo
authors.forename: He-Sheng
authors.initials: HS
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002397:Catfishes; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics*; D013006:Growth Hormone / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D013006:Growth Hormone