An uncommon H3/Ser10 phosphorylation pattern in Cestrum strigilatum (Solanaceae), a species with B chromosomes.
Cestrum strigilatum (Solanaceae) is a South American shrub with B chromosomes. Bs show a univalent behavior when a single B is present, have non-Mendelian segregation, and are poor in genes and rich in repetitive DNA. In this study, the histone H3 at serine 10 (H3/Ser10) phosphorylation pattern was investigated during mitosis and meiosis of C. strigilatum collected from the wild and was compared in A and B chromosomes. The results revealed that H3/Ser10 phosphorylation of A chromosomes occurred only in the pericentromeric region in both mitosis and meiosis, whereas in the B univalent, phosphorylation appeared in almost the whole extent of the chromosome, except in the terminal portion of the long arm. In meiosis II, the phosphorylation of A chromosomes was similar to that in the first division of meiosis, but the Bs did not show H3/Ser10 phosphorylation. Our results suggest that phosphorylation at the pericentromeric region may be associated with chromosome motility during cell divisions and with the cohesion of B chromatids in a univalent structure in meiosis I.
Fernandes
Thiago
T
Laboratório de Biodiversidade e Restauração de Ecossistemas, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Caixa Postal 6001, CEP 86051-990, Londrina, PR, Brazil.
Yuyama
Priscila Mary
PM
Moraes
Ana Paula
AP
Vanzela
André Luís Laforga
AL
D032461:Chromosomes, Plant; D006657:Histones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D012694:Serine / Q000235:genetics*; D019657:Solanaceae / Q000235:genetics*
D006657:Histones; D012694:Serine