Expanding substrate specificity of nucleoside 2'-deoxyribosyltransferase.
Nucleoside 2'-deoxyribosyltransferase (NDT) is used to synthesize unnatural 2'-deoxyribonucleosides, modified mostly on the heterocyclic base. Here we describe a strategy for improving 2,3-dideoxyribosyl(ddR) transfer activity of NDT by combining mutagenesis and in vivo selection in E. coli.
Kaminski
Pierre Alexandre
PA
Institut Pasteur, Unité de Chimie Organique, CNRS, URA2128, 28, rue du Dr Roux, 75724 Paris cedex 15, France. akaminsk@pasteur.fr
Dugué
Laurence
L
Pochet
Sylvie
S
D004926:Escherichia coli / Q000235:genetics; D052197:Limosilactobacillus fermentum / Q000201:enzymology; D052199:Lactobacillus leichmannii / Q000201:enzymology; D016296:Mutagenesis; D010430:Pentosyltransferases / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
D010430:Pentosyltransferases; C021888:nucleoside deoxyribosyltransferase