Expression patterns of betaig-h3 in chondrocyte differentiation during endochondral ossification.
betaig-h3 is a TGF-beta-induced extracellular matrix protein which is expressed in many tissues including bones and cartilages. In previous reports, we showed that betaig-h3 mediates cell adhesion and migration and, especially in bones, negatively regulates the mineralization in the end stage of endochondral ossification. Here, to elucidate the expression pattern and role of betaig-h3 in chondrocyte differentiation, ATDC5 chondrocytes and embryonic and postnatal mice were used for in vitro differentiation studies and in vivo studies, respectively. betaig-h3 was strongly induced by the treatment of TGF-beta1 and the expression level of betaig-h3 mRNA and protein were highly expressed in the early stages of differentiation but decreased in the late stages in ATDC5. Furthermore, the patterns of TGF-beta1, -beta2, and -beta3 mRNA expression were concurrent with betaig-h3 in ATDC5. betaig-h3 was deeply stained in perichondrium (PC), periosteum (PO), and prehypertrophic chondrocytes (PH) through the entire period of endochondral ossification in mice. betaig-h3 was mainly expressed in PC and PH at embryonic days and obviously in PH in postnatal days. These results suggest that betaig-h3 may play a critical role as a regulator of chondrogenic differentiation in endochondral ossification.
Han
Min-Su
MS
Cell and Matrix Research Institute, Department of Biochemistry and Cell Biology, Kyungpook National University School of Medicine, Daegu 700-422, Korea.
Kim
Jung-Eun
JE
Shin
Hong-In
HI
Kim
In-San
IS
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D019902:Chondrocytes / Q000378:metabolism*; D020219:Chondrogenesis / Q000235:genetics*; D004622:Embryo, Mammalian; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005269:Femur / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008813:Mice, Inbred ICR; D010012:Osteogenesis / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000099222:betaIG-H3 Protein
D016326:Extracellular Matrix Proteins; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein
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