A technological update of molecular diagnostics for infectious diseases.
Identification of a causative pathogen is essential for the choice of treatment for most infectious diseases. Many FDA approved molecular assays; usually more sensitive and specific compared to traditional tests, have been developed in the last decade. A new trend of high throughput and multiplexing assays are emerging thanks to technological developments for the human genome sequencing project. The applications of microarray and ultra high throughput sequencing technologies for diagnostic microbiology are reviewed. The race for the $1000 genome technology by 2014 will have a profound impact in diagnosis and treatment of infectious diseases in the near future.
Liu
Yu-Tsueng
YT
Moores Cancer Center, University of California, San Diego, 3855 Health Sciences Drive, 0815, La Jolla, CA 92093, USA. ytliu@ucsd.edu
D003141:Communicable Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D004269:DNA, Bacterial / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008828:Microbiological Techniques; D025202:Molecular Diagnostic Techniques; D020411:Oligonucleotide Array Sequence Analysis; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004279:DNA, Viral
Lab Chip. 2007 Jan;7(1):41-57
17180204
Biosens Bioelectron. 2007 Feb 15;22(7):1205-17
16934970
Sci Am. 2006 Jan;294(1):46-54
16468433
J Clin Virol. 2005 Mar;32 Suppl 1:S1-6
15753006
Diagn Cytopathol. 2001 Mar;24(3):186-92
11241902
Curr Opin Biotechnol. 2007 Jun;18(3):267-72
17467973
AIDS. 2006 Feb 14;20(3):474-5
16439890
Dermatol Online J. 2005 Dec 01;11(3):16
16409912
Crit Rev Microbiol. 2005;31(2):91-9
15988839
Curr Opin Microbiol. 2004 Jun;7(3):245-54
15196491
Emerg Infect Dis. 2007 Jan;13(1):73-81
17370518
Science. 2003 May 30;300(5624):1394-9
12730500
Science. 1994 Dec 16;266(5192):1865-9
7997879
Clin Microbiol Rev. 2006 Jan;19(1):165-256
16418529
Lancet Infect Dis. 2004 Feb;4(2):100-11
14871635
Va Med Q. 1992 Fall;119(4):256-8
1390976
Am J Obstet Gynecol. 2006 Jun;194(6):1532-6
16731068
J Pathol. 2006 Jan;208(2):165-75
16362995
Trends Genet. 2008 Mar;24(3):133-41
18262675
N Engl J Med. 2006 May 25;354(21):2235-49
16723615
Diagn Cytopathol. 2007 May;35(5):293-5
17427219
HIV Med. 2004 Nov;5(6):452-4
15544700
Transplantation. 2004 Feb 27;77(4):574-9
15084938
Cancer Res. 1971 Aug;31(8):1066-8
4999485
Nat Methods. 2008 Jan;5(1):15
18175411
Science. 2008 Feb 22;319(5866):1096-100
18202256
Cancer Epidemiol Biomarkers Prev. 1999 Feb;8(2):153-8
10067813
Lancet. 2002 Feb 9;359(9305):497-8
11853800
AIDS Patient Care STDS. 2007 Jul;21(7):447-51
17651025
Nature. 2007 Oct 18;449(7164):835-42
17943120
Clin Infect Dis. 2006 Oct 15;43(8):e71-6
16983602
J Clin Virol. 2004 Jan;29(1):2-12
14675863
Br J Dermatol. 2000 Mar;142(3):525-8
10735964
Leuk Lymphoma. 1996 Feb;20(5-6):509-11
8833412
Annu Rev Public Health. 2005;26:281-302
15760290
Virology. 2004 Jun 20;324(1):17-27
15183049
J R Soc Med. 2003 May;96(5):238-9
12724438
Cancer. 1978 Nov;42(5):2311-21
719609
Br J Dermatol. 2002 Apr;146(4):671-3
11966703
Cell. 2008 Mar 7;132(5):721-3
18329356
Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
9843981
Br J Dermatol. 2002 May;146(5):895-8
12000392
PLoS Biol. 2003 Nov;1(2):E2
14624234
J Am Acad Dermatol. 2001 Aug;45(2):309-12
11464198
J Clin Microbiol. 2002 Mar;40(3):779-87
11880393
Nat Rev Genet. 2004 May;5(5):335-44
15143316
Genome Res. 2005 Dec;15(12):1767-76
16339375
South Med J. 2000 Sep;93(9):920-2
11005357
Clin Infect Dis. 2007 Sep 1;45 Suppl 2:S99-S111
17683022
J Gen Virol. 1999 Jul;80 ( Pt 7):1725-1733
10423141
N Engl J Med. 2008 Mar 6;358(10):991-8
18256387
Lancet Infect Dis. 2004 Jun;4(6):337-48
15172342
Clin Chem. 2007 Nov;53(11):2002-9
17890442
J Clin Microbiol. 2007 Jul;45(7):2340-3
17494722
Clin Microbiol Rev. 2007 Jan;20(1):49-78
17223623
Nat Biotechnol. 2007 Feb;25(2):149
17287734