Dynamics of 2-N-tert-butylaminoxyladenosine incorporated into oligodeoxynucleotides.
We synthesized oligodeoxynucleotide (ODN, 3) containing 2-N-tert-butylaminoxyladenosine (1) and studied EPR spectra of 1 and its duplexes with varied sequences. Duplex formation resulted in a broadening of spectrum and a significant decrease in peak-to-peak height and peak height ratio values. The h(+)/ho values in EPR spectra well correlated with stability of duplex which indicated ODN containing 1 has the potential to monitor DNA structure.
Aso
Mariko
M
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. aso@phar.kyushu-u.ac.jp
Kurita
Manami
M
Mirc
John Walter
JW
Kaneko
Toshiyuki
T
Higuchi
Yoshitaka
Y
Koga
Noboru
N
Suemune
Hiroshi
H
D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013113:Spin Labels
C520181:2-N-tert-butylaminooxyladenosine; D009838:Oligodeoxyribonucleotides; D013113:Spin Labels; D000241:Adenosine