title: Improvement of transcription- and transfection-efficiency by synthetic polyampholytes.
abstract: Water-soluble PEG derivatives having both amino- and carboxyl-pendants (PEG-ACs) were synthesized, and examined for their transcription- and transfection-enhancing activity on DNA/polycation complexes. PEG-AC could be deposited onto the surface of DNA/polyethylenimine(PEI) complexes, and enhanced their transcriptional activity. Fluorescence anisotropy study showed that the amphoteric PEG-AC loosened the tightly compacted DNA/PEI complex to facilitate the approach of transcriptional factors. Transcriptional activity of the complex was in good correlation with its transgene expression efficiency, indicating the importance of improvement in transcription for effective gene transfection.
authors.affiliation: Otsuma Women's University Graduate School, Chiyoda-ku, Tokyo, Japan. d1107101@cst.otsuma.ac.jp
authors.forename: Chieko
authors.initials: C
authors.lastname: Yoshihara
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ito
authors.forename: Chwen-Yang
authors.initials: CY
authors.lastname: Shew
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Koyama
mesh_terms: D000818:Animals; D000838:Anions / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D000838:Anions; D002412:Cations; D011092:Polyethylene Glycols; D004247:DNA