Selective recognition of a tetra-amino-acid motif containing phosphorylated tyrosine residue by ribonucleopeptide.
We describe here a ribonucleopeptide (RNP) receptor targeting a tetra-amino-acid motif containing phosphotyrosine, GpYSR. GpYSR-binding RNP receptors were obtained from an RNA-based RNP library by in vitro selection. These receptors have a higher affinity than those of previously obtained pY-binding RNP receptors. One of these RNP receptors exhibited unique specificity to the target GpYSR peptide over other tetra-amino-acid peptides derived from the tyrosine-phosphorylation sites of native proteins. The GpYSR-binding RNP receptor discriminated not only the phosphorylated tyrosine residue, but also its surrounding three amino acid residues. Thus, RNP receptors could target a defined pY-containing amino-acid sequence by expanding the recognition surface within the ligand-binding pocket of RNP.
Nakano
Shun
S
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
Hasegawa
Tetsuya
T
Fukuda
Masatora
M
Fujieda
Nobutaka
N
Tainaka
Kazuki
K
Morii
Takashi
T
D020816:Amino Acid Motifs; D010455:Peptides / Q000737:chemistry*; D019000:Phosphotyrosine / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
D010455:Peptides; D019000:Phosphotyrosine; D012313:RNA