title: Protective effect of bioactive ceramics on liver injury: regulation of pro-inflammatory cytokins expression.
abstract: OBJECTIVE
  A bioactive ceramics has been reported to regulate the expression of inflammatory cytokines in macrophage cells activated by lipopolysaccharides (LPS). In present study, we investigated the anti-inflammatory effect of bioactive ceramics using liver injury model in mouse.
  
  
  METHODS
  Mice were divided into three groups: Normal group, LPS group (LPS and no ceramics treatment), Ceramics group (LPS and ceramics treatment).
  
  
  RESULTS
  LPS administration induced the increase of plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in mouse. The losses of cytoplasm of hepatocytes due to LPS caused the increase of AST and ALT in mouse plasma. In Ceramics group, however, the concentration of AST and ALT were much lower than LPS group until 6 weeks. And the losses of cytoplasm were rarely seen in Ceramics group. RT-PCR results showed that the decrease of proinflammatory cytokines such as IL-1beta and IL-6 was observed in Ceramics group. Moreover, TGF-beta1 and VEGF expression was increased in Ceramics group.
  
  
  CONCLUSIONS
  Bioactive ceramics effectively protected endotoxin-induced liver injury by attenuation of inflammatory processes in mice.
authors.affiliation: Research Institute of Biomedical Engineering, School of Medicine, Catholic University of Daegu, Daegu, South Korea.
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Lee
authors.forename: Kwan-Kyu
authors.initials: KK
authors.lastname: Park
authors.forename: Kyung-Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Young-Ae
authors.initials: YA
authors.lastname: Chu
authors.forename: Jae-Pil
authors.initials: JP
authors.lastname: Jeon
authors.forename: Meeyul
authors.initials: M
authors.lastname: Hwang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001483:Base Sequence; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002516:Ceramics / Q000737:chemistry* / Q000494:pharmacology; D016207:Cytokines / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008422:Materials Testing; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D017931:DNA Primers; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
references.citation: J Biomed Mater Res. 1973;7(3):25-42
references.pmid: 4123968
references.citation: Science. 2002 Feb 8;295(5557):1014-7
references.pmid: 11834817
references.citation: J Hepatol. 2002 Nov;37(5):678-80
references.pmid: 12399237
references.citation: Science. 1984 Nov 9;226(4675):630-6
references.pmid: 6093253
references.citation: Rheumatol Int. 2005 Dec;26(2):168-72
references.pmid: 15965635
references.citation: Altern Med Rev. 2002 Aug;7(4):328-35
references.pmid: 12197784
references.citation: Ann N Y Acad Sci. 1988;523:54-71
references.pmid: 2837945
references.citation: Science. 1990 Sep 21;249(4975):1431-3
references.pmid: 1698311
references.citation: Arch Biochem Biophys. 2003 Mar 1;411(1):63-72
references.pmid: 12590924
references.citation: J Biomed Mater Res A. 2007 Mar 1;80(3):513-9
references.pmid: 17133451
references.citation: Immunopharmacology. 1995 Apr;29(3):187-205
references.pmid: 7542643