title: Novel base-functionalized DNA. Efficient methodology for construction and bioanalytical applications.
abstract: A novel efficient two-step methodology for the construction of base-functionalized DNA is based on direct aqueous cross-coupling reactions of unprotected nucleoside triphosphates followed by polymerase incorporation. Preliminary applications of the modified DNA in electrochemical detection and bioanalysis are outlined.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, CZ-16610 Prague 6, Czech Republic. hocek@uochb.cas.cz
authors.forename: Michal
authors.initials: M
authors.lastname: Hocek
authors.forename: Milan
authors.initials: M
authors.lastname: Vrábel
authors.forename: Hana
authors.initials: H
authors.lastname: Cahová
authors.forename: Jan
authors.initials: J
authors.lastname: Riedl
authors.forename: Lubica
authors.initials: L
authors.lastname: Kalachová
authors.forename: Hana
authors.initials: H
authors.lastname: Pivonková
authors.forename: Petra
authors.initials: P
authors.lastname: Horáková
authors.forename: Ludek
authors.initials: L
authors.lastname: Havran
authors.forename: Miroslav
authors.initials: M
authors.lastname: Fojta
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D010084:Oxidation-Reduction
chemical_list: D003854:Deoxyribonucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase