Antioxidant capacity of flavonoid in soybean seedlings under the joint actions of rare earth element La(III) and ultraviolet-B stress.
The dynamic state of antioxidant capacity of flavonoid was investigated for a further demonstration of alleviating the damage of the ultraviolet (UV)-B radiation in the La-treated soybean seedlings under UV-B stress. Using hydroponics culture, the effects of lanthanum on the contents of flavonoid and its ability of antioxidant under elevated ultraviolet-B radiation (280-320 nm) was studied. The results showed flavonoid content in soybean seedlings with UV-B treatment during the stress and convalescent period was increased initially and then decreased, compared with control. Membrane permeability and MDA contents increase at first (first to fifth day) and then decrease (6th-11th day). A similar change of flavonoid content and clearance of flavonoid scavenging O2- and *OH in soybean seedlings occurred. Flavonoid content and ability of flavonoid scavenging O2- and *OH in soybean seedlings with La(III) + UV-B treatment were higher than those of UV-B treatment. Meanwhile, membrane permeability and MDA contents in soybean seedlings were lower than those of UV-B treatment. Compared with control, phenylalanine content in soybean seedlings with UV-B treatment is depressed, phenylalanine content in soybean seedlings with La(III) treatment was enhanced. However, phenylalanine content in La(III) + UV-B treatment is not decreased but slightly increased, compared with UV-B treatment. It suggested that the regulative effect of La(III) of the optimum concentration on flavonoid improved the metabolism of ROS, diminished the concentration of MDA and maintained normal plasma membrane permeability, and that its protective effect against low UV-B radiation is superior to that of high UV-B radiation. The defensive effect of La(III) on soybean seedlings under UV-B stress is carried out on the layer of defense system.
Peng
Qi
Q
School of Environment and Civil Engineering, Jiangnan University, Wuxi, 214122, People's Republic of China.
Zhou
Qing
Q
D000975:Antioxidants / Q000378:metabolism; D005419:Flavonoids / Q000378:metabolism*; D007811:Lanthanum / Q000633:toxicity*; D008674:Metals, Rare Earth / Q000633:toxicity; D036226:Seedlings / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D013025:Glycine max / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D000975:Antioxidants; D005419:Flavonoids; D008674:Metals, Rare Earth; D007811:Lanthanum