title: Quantitative analysis of the bacteriophage Qbeta infection cycle.
abstract: In this study, the infection cycle of bacteriophage Qbeta was investigated. Adsorption of bacteriophage Qbeta to Escherichia coli is explained in terms of a collision reaction, the rate constant of which was estimated to be 4x10(-10) ml/cells/min. In infected cells, approximately 130 molecules of beta-subunit and 2x10(5) molecules of coat protein were translated in 15 min. Replication of Qbeta RNA proceeded in 2 steps-an exponential phase until 20 min and a non-exponential phase after 30 min. Prior to the burst of infected cells, phage RNAs and coat proteins accumulated in the cells at an average of up to 2300 molecules and 5x10(5) molecules, respectively. An average of 90 infectious phage particles per infected cell was released during a single infection cycle up to 105 min.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Tsukada
authors.forename: Misato
authors.initials: M
authors.lastname: Okazaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kita
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Inokuchi
authors.forename: Itaru
authors.initials: I
authors.lastname: Urabe
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yomo
mesh_terms: D017908:Allolevivirus / Q000502:physiology*; D036022:Capsid Proteins / Q000096:biosynthesis; D004926:Escherichia coli / Q000821:virology*; D016679:Genome, Viral; D012367:RNA, Viral / Q000096:biosynthesis; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral