Microsphere surface protein determination using flow cytometry.
This unit describes an extrinsic staining protocol using the amine-reactive CBQCA dye to measure the amount of protein on the surface of a microsphere. This approach is novel in that it allows microspheres bearing proteins without known binding partners to be accurately quantified on a flow cytometer.
Woods
Travis A
TA
Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
Graves
Steven W
SW
Nolan
John P
JP
D001565:Benzoates; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D008565:Membrane Proteins / Q000737:chemistry*; D008863:Microspheres; D011804:Quinolines; D013499:Surface Properties
D001565:Benzoates; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D011804:Quinolines; C069617:3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde