title: Freeze-fracture replica immunolabelling reveals caveolin-1 in the human cardiomyocyte plasma membrane.
abstract: Mutations in or ablation of the gene encoding caveolin-3, a protein of muscle cell caveolae, result in forms of muscular dystrophy and cardiomyopathy. Another member of the caveolin gene family, caveolin-1, is widely considered not to be expressed in myocytes, yet ablation of the gene encoding this protein in mice also results in cardiomyopathy. By applying the high-resolution electron-microscopical imaging technique of freeze-fracture replica immunolabelling, we report here evidence that caveolin-1 is expressed in human cardiac myocytes, localized to both caveolae and non-caveolar domains in the plasma membrane. Disorders of the myocyte resulting from defects in caveolin-1 may thus arise directly, at the level of the myocyte, rather than via other cell types as previously proposed.
authors.affiliation: Leibniz-Institute for Arteriosclerosis Research, Department of Cell Biology and Ultrastructure Research, University of Münster, Münster, Germany. robenek@uni-muenster.de
authors.forename: Horst
authors.initials: H
authors.lastname: Robenek
authors.forename: Gabriele
authors.initials: G
authors.lastname: Weissen-Plenz
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Severs
mesh_terms: D021941:Caveolae / Q000378:metabolism / Q000648:ultrastructure; D051242:Caveolin 1 / Q000378:metabolism*; D051244:Caveolin 3 / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D005614:Freeze Fracturing; D006801:Humans; D007150:Immunohistochemistry; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000648:ultrastructure*; D016628:Replica Techniques
chemical_list: C495540:CAV1 protein, human; C417736:CAV3 protein, human; D051242:Caveolin 1; D051244:Caveolin 3
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