In vivo bioluminescence imaging in an experimental mouse model for dendritic cell based immunotherapy against malignant glioma.
The value of bioluminescence imaging (BLI) for experimental cancer models has become firmly established. We applied BLI to the GL261 glioma model in the context of dendritic cell (DC) immunotherapy. Initial validation revealed robust linear correlations between in vivo, ex vivo and in vitro luciferase activity measurements. Ex vivo BLI demonstrated midline crossing and leakage of tumor cells. Orthotopically challenged mice followed with BLI showed an initial adaptation phase, after which imaging data correlated linearly with stereologically determined tumor dimensions. Transition from healthy to moribund state corresponded with an increasing in vivo flux but the onset of neurological deficit was clearly delayed compared to the onset of in vivo flux increase. BLI was implemented in prophylactic immunotherapy and imaging data were prognostic for therapy outcome. Three distinct response patterns were detected. Our data underscore the feasibility of in vivo BLI in an experimental immunotherapeutic setting in the GL261 glioma model.
Maes
W
W
Department of Experimental Medicine, Katholieke Universiteit Leuven, Leuven, Belgium.
Deroose
C
C
Reumers
V
V
Krylyshkina
O
O
Gijsbers
R
R
Baekelandt
V
V
Ceuppens
J
J
Debyser
Z
Z
Van Gool
S W
SW
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000628:therapy*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology*; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005910:Glioma / Q000175:diagnosis* / Q000628:therapy*; D007167:Immunotherapy / Q000379:methods*; D016014:Linear Models; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation / Q000379:methods; D015203:Reproducibility of Results; D016019:Survival Analysis; D013997:Time Factors; D014161:Transduction, Genetic / Q000379:methods
D000951:Antigens, Neoplasm; D008156:Luciferases
Mol Imaging. 2004 Apr;3(2):117-24
15296676
Genes Dev. 2003 Mar 1;17(5):545-80
12629038
J Immunol Methods. 1999 Feb 1;223(1):77-92
10037236
J Exp Med. 1992 Dec 1;176(6):1693-702
1460426
Int J Cancer. 2007 Jul 1;121(1):95-105
17315190
Neoplasia. 2005 Aug;7(8):730-40
16207475
J Surg Res. 2004 Aug;120(2):249-55
15234220
Mol Imaging Biol. 2006 Nov-Dec;8(6):315-23
17053862
Eur J Cancer. 2006 Dec;42(18):3294-303
17027258
J Neurosurg. 2005 Apr;102(4):738-44
15871520
J Neurooncol. 2001 Jan;51(1):1-9
11349874
J Neurosurg. 2008 Feb;108(2):304-10
18240927
J Neurosurg. 2007 Feb;106(2):299-305
17410715
Cancer Immunol Immunother. 2006 Dec;55(12):1491-503
16485128
Neurol Res. 2006 Jul;28(5):527-31
16808884
Nat Med. 1998 Feb;4(2):245-7
9461201
Photochem Photobiol. 1997 Oct;66(4):523-31
9337626
Clin Cancer Res. 2006 Sep 15;12(18):5288-97
17000661
Nat Rev Cancer. 2002 Jan;2(1):11-8
11902581
J Nucl Med. 2007 Feb;48(2):295-303
17268028
Eur J Cancer. 2004 Apr;40(6):890-8
15120044
Clin Cancer Res. 2006 Aug 1;12(15):4730-7
16899624
J Magn Reson Imaging. 2002 Oct;16(4):378-87
12353253
Clin Exp Metastasis. 2007;24(5):389-401
17541709
Clin Exp Metastasis. 2003;20(8):757-66
14713109
Cancer Res. 2007 Aug 1;67(15):7525-33
17671224
Clin Cancer Res. 2006 Jul 15;12(14 Pt 1):4294-305
16857805
Neoplasia. 2000 Jan-Apr;2(1-2):41-52
10933067
Neurosurgery. 2006 Feb;58(2):365-72; discussion 365-72
16462491
DNA Cell Biol. 2005 May;24(5):317-24
15869409
Neuroscience. 2005;130(4):813-31
15652981
Genet Vaccines Ther. 2004 Aug 4;2(1):7
15294018
Cancer Gene Ther. 2003 Sep;10(9):678-88
12944987
Mol Ther. 2006 Sep;14(3):423-31
16820324
J Neurosci. 2008 Apr 23;28(17):4406-13
18434519
J Pediatr Surg. 2007 Jul;42(7):1172-9
17618876
Transplantation. 2006 Apr 27;81(8):1179-84
16641605
Cancer Immunol Immunother. 2008 Sep;57(9):1279-89
18253732
Neuroscience. 2004;129(4):983-91
15561413
Gene Ther. 2004 Nov;11(21):1551-8
15343358
Invest Ophthalmol Vis Sci. 2005 May;46(5):1581-7
15851554
J Nucl Med. 2007 Mar;48(3):445-54
17332623
Clin Exp Metastasis. 2006;23(1):19-31
16715352
Cancer Res. 2007 Oct 1;67(19):9389-97
17909048
Eur J Cancer. 2002 Nov;38(16):2128-36
12387838
Clin Exp Metastasis. 2003;20(8):733-44
14713107
Scand J Immunol. 1993 Dec;38(6):499-507
7504825
Expert Opin Biol Ther. 2003 Oct;3(7):1163-72
14519079
BJU Int. 2008 Jan;101(1):120-4
17888045
Biotechnol Lett. 2007 Nov;29(11):1665-70
17609854
Int J Cancer. 2004 Sep 20;111(5):777-82
15252850
Oncogene. 2003 Oct 9;22(44):6865-72
14534533
Cancer Sci. 2006 Jun;97(6):546-53
16734735
Glia. 2006 Nov 1;54(6):526-35
16906541
Cancer Cell. 2003 Aug;4(2):133-46
12957288
J Invest Dermatol. 2005 Jul;125(1):159-165
15982316
Eur J Immunol. 2006 Oct;36(10):2807-16
17013976
Mol Ther. 2005 Nov;12(5):778-88
16150649
Cancer Gene Ther. 2002 Sep;9(9):787-97
12189529
Exp Hematol. 2007 Mar;35(3):407-15
17309821
Neoplasia. 2000 Nov-Dec;2(6):491-5
11228541
J Neurosurg. 2006 Sep;105(3):430-7
16961139
J Clin Invest. 2008 Apr;118(4):1390-7
18357341