Chemiluminescence determination of chlorpheniramine using tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system and sequential injection analysis.
A sequential injection (SI) method was developed for the determination of chlorpheniramine (CPA), based on the reaction of this drug with tris(1,10-phenanthroline)-ruthenium(II) [Ru(phen)(3)(2+)] and peroxydisulphate (S(2)O(8)(2-)) in the presence of light. The instrumental set-up utilized a syringe pump and a multiposition valve to aspirate the reagents [Ru(phen)(3)(2+) and S(2)O(8)(2-)] and a peristaltic pump to propel the sample. The experimental conditions affecting the chemiluminescence reaction were systematically optimized, using the univariate approach. Under the optimum conditions linear calibration curves of 0.1-10 microg/ml were obtained. The detection limit was 0.04 microg/ml and the relative standard deviation (RSD) was always < 5%. The procedure was applied to the analysis of CPA in pharmaceutical products and was found to be free from interferences from concomitants usually present in these preparations.
Suliman
Fakhr Eldin O
FE
Department of Chemistry, College of Science, Sultan Qaboos University, Box 36, Al-Khod 123, Sultanate of Oman. fsuliman@squ.edu.om
Al-Hinai
Mohammed M
MM
Al-Kindy
Salma M Z
SM
Salama
Salama B
SB
D000645:Ammonium Sulfate / Q000032:analysis* / Q000737:chemistry; D002744:Chlorpheniramine / Q000032:analysis* / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods*; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000032:analysis* / Q000737:chemistry; D010618:Phenanthrolines / Q000032:analysis* / Q000737:chemistry
D009942:Organometallic Compounds; D010618:Phenanthrolines; C034282:tris-(1,10-phenanthroline)ruthenium; C031276:ammonium peroxydisulfate; D002744:Chlorpheniramine; D000645:Ammonium Sulfate