title: Response of a solid-liquid two-phase partitioning bioreactor to transient BTEX loadings.
abstract: A two-phase partitioning bioreactor (TPPB) consisting of an aqueous phase containing a bacterial consortium and a polymeric phase of silicone rubber pellets (solid volume fraction 0.1) was used to treat a gaseous waste stream containing benzene, toluene, ethylbenzene and o-xylene (BTEX). The function of the solid polymer phase was to absorb/desorb the gaseous volatile organic compounds providing a buffering effect to protect the cells from high transient loadings and to sequester the BTEX for subsequent degradation. The TPPB was subjected to high and fluctuating inlet loadings of BTEX in the form of 4h step changes of 2, 4, 6 and 10 times the nominal inlet loading of 60 gm(-3) h(-1) total BTEX in approximately equal amounts, and removal efficiencies and elimination capacities were determined. It was found that overall removal efficiencies of greater than 95% can be achieved while obtaining overall elimination capacities of up to 282 gm(-3) h(-1) during transient operation and TPPB operation succumbs to toxic substrate levels between step changes of 6 and 10 times the nominal loading value (360-600 gm(-3) h(-1)). BTEX concentrations in the aqueous phase and the polymer phase of the TPPB were monitored throughout the imposed step changes to determine the extent to which the sequestering phase can buffer the aqueous phase from BTEX. With the polymer phase comprising only 10% of the total working volume of the reactor, the polymer beads accounted for up to 93%, 91% and 70% of the total BTEX present in the working volume for step changes of 2, 4 and 6 times the nominal loading, respectively.
authors.affiliation: Department of Chemical Engineering, Queen's University, Kingston, Ontario, Canada K7L 3N6.
authors.forename: Jennifer V
authors.initials: JV
authors.lastname: Littlejohns
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Daugulis
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001554:Benzene / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D001555:Benzene Derivatives / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D019149:Bioreactors; D059013:Microbial Consortia; D044367:Phase Transition; D014050:Toluene / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D018505:Waste Management / Q000379:methods*; D014992:Xylenes / Q000032:analysis / Q000378:metabolism / Q000633:toxicity
chemical_list: D001555:Benzene Derivatives; D014992:Xylenes; D014050:Toluene; D001554:Benzene; C004912:ethylbenzene