[Detection of bone marrow-derived cells in neointimal thickening in the rat carotid artery by nested polymerase chain reaction].
The bone marrow origin of cells involved in neointimal formation after injury of the luminal surface of the vessel was confirmed by highly sensitive nested polymerase chain reaction on isolated vascular wall cells. The model of intimal hyperplasia after balloon angioplasty of the carotid artery in radiation bone marrow chimeras between male and female Wistar rats was used. The Y chromosomes of rat male donors of the bone marrow for irradiated females were used as a marker of bone marrow-derived cells. This approach demonstrated a bone marrow origin of a large fraction of alpha-actin-positive (smooth muscle) neointimal cells.
Il'inskaia
O P
OP
Antropova
Iu G
IuG
Kalinina
N I
NI
Mishina
V A
VA
Radiukhina
N V
NV
Tararak
E M
EM
D000800:Angioplasty, Balloon; D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology*; D016026:Bone Marrow Transplantation; D002339:Carotid Arteries / Q000473:pathology*; D020212:Carotid Artery Injuries / Q000473:pathology; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction; D011828:Radiation Chimera; D051381:Rats; D017208:Rats, Wistar; D017539:Tunica Intima / Q000473:pathology*; D014998:Y Chromosome / Q000235:genetics