title: ELISA and multiplex technologies for cytokine measurement in inflammation and aging research.
abstract: Over the last decade there has been an enormous expansion of research focused on defining the role of inflammation in aging, age-related diseases, disability, and frailty. The availability of methods to measure cytokines and other inflammatory mediators or markers with high sensitivity and specificity is critically important. Enzyme-linked immunosorbent assay (ELISA), the most widely used and best validated method, is limited by its ability to measure only a single protein in each sample. Recent developments in serum cytokine quantification technology include multiplex arrays, which offer the potential of better evaluating the complexity and dynamic nature of inflammatory responses and offer substantial cost and sample savings over traditional ELISA measurements. Despite potential advantages of this new technology, experience with these techniques is limited, and it has not emerged to date as the gold standard in inflammatory mediator measurement. This article reviews ELISA and the emerging multiplex technologies, compares the cost and effectiveness of recently developed multiplex arrays with traditional ELISA technology, and provides specific recommendations for investigators interested in measuring serum inflammatory mediators in older adults.
authors.affiliation: UConn Center on Aging, University of Connecticut Health Center, 263 Farmington, Farmington, CT 06030-5215, USA.
authors.forename: Sean X
authors.initials: SX
authors.lastname: Leng
authors.forename: Janet E
authors.initials: JE
authors.lastname: McElhaney
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Walston
authors.forename: Dongxu
authors.initials: D
authors.lastname: Xie
authors.forename: Neal S
authors.initials: NS
authors.lastname: Fedarko
authors.forename: George A
authors.initials: GA
authors.lastname: Kuchel
mesh_terms: D003365:Costs and Cost Analysis; D016207:Cytokines / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005853:Geriatrics; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000379:methods*; D018836:Inflammation Mediators / Q000032:analysis*; D012106:Research; D012680:Sensitivity and Specificity
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators
references.citation: J Immunol Methods. 2004 Aug;291(1-2):27-38
references.pmid: 15345302
references.citation: Am J Med. 2003 Oct 15;115(6):501-2
references.pmid: 14563508
references.citation: J Biol Chem. 1994 Jan 14;269(2):1533-40
references.pmid: 7507109
references.citation: Methods. 2006 Apr;38(4):317-23
references.pmid: 16481199
references.citation: Biogerontology. 2006 Oct-Dec;7(5-6):449-59
references.pmid: 16967203
references.citation: Mech Ageing Dev. 2007 Jan;128(1):92-105
references.pmid: 17116321
references.citation: Clin Diagn Lab Immunol. 1999 Jan;6(1):89-95
references.pmid: 9874670
references.citation: Cytometry B Clin Cytom. 2004 Sep;61(1):35-9
references.pmid: 15351980
references.citation: Aging Clin Exp Res. 2004 Jun;16(3):240-3
references.pmid: 15462468
references.citation: J Gerontol A Biol Sci Med Sci. 2006 Jun;61(6):575-84
references.pmid: 16799139
references.citation: Mech Ageing Dev. 2007 Jul-Aug;128(7-8):450-4
references.pmid: 17644159
references.citation: J Am Geriatr Soc. 2007 Jun;55(6):864-71
references.pmid: 17537086
references.citation: Nature. 2002 Dec 19-26;420(6917):846-52
references.pmid: 12490957
references.citation: J Immunol. 1989 Jan 1;142(1):148-52
references.pmid: 2462586
references.citation: Crit Care. 2007;11(2):R49
references.pmid: 17448250
references.citation: J Gerontol A Biol Sci Med Sci. 2008 Feb;63(2):184-9
references.pmid: 18314455
references.citation: J Interferon Cytokine Res. 2000 Feb;20(2):125-31
references.pmid: 10714547
references.citation: Am J Clin Pathol. 1974 Oct;62(4):545-52
references.pmid: 4415749
references.citation: J Am Geriatr Soc. 1993 Feb;41(2):176-81
references.pmid: 8426042
references.citation: Am J Clin Pathol. 1974 Jan;61(1):108-13
references.pmid: 4809144
references.citation: Nature. 1959 Nov 21;184 (Suppl 21):1648-9
references.pmid: 13846363