Immunophenotypic analysis of peripheral blood lymphocytes.
This unit on basic phenotyping describes two basic and two alternate protocols for the immunophenotypic identification and classification of human peripheral blood lymphocytes. The presented protocols comply with consensus recommendations from professional organizations that regulate the clinical use of such assays. The authors discuss whole blood assay systems as well as enriched systems from several perspectives, including absolute number determination.
Ruiz-Argüelles
A
A
Laboratorios Clínicos de Puebla, Puebla, Mexico.
Pérez-Romano
B
B
D000911:Antibodies, Monoclonal / Q000737:chemistry; D002452:Cell Count; D002469:Cell Separation; D004912:Erythrocytes / Q000166:cytology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D016131:Lymphocyte Subsets / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism
D000911:Antibodies, Monoclonal