title: Validation of an NSP-based (negative selection pattern) gene family identification strategy.
abstract: BACKGROUND
  Gene family identification from ESTs can be a valuable resource for analysis of genome evolution but presents unique challenges in organisms for which the entire genome is not yet sequenced. We have developed a novel gene family identification method based on negative selection patterns (NSP) between family members to screen EST-generated contigs. This strategy was tested on five known gene families in Arabidopsis to see if individual paralogs could be identified with accuracy from EST data alone when compared to the actual gene sequences in this fully sequenced genome.
  
  
  RESULTS
  The NSP method uniquely identified family members in all the gene families tested. Two members of the FtsH gene family, three members each of the PAL, RF1, and ribosomal L6 gene families, and four members of the CAD gene family were correctly identified. Additionally all ESTs from the representative contigs when checked against MapViewer data successfully identify the gene locus predicted.
  
  
  CONCLUSIONS
  We demonstrate the effectiveness of the NSP strategy in identifying specific gene family members in Arabidopsis using only EST data and we describe how this strategy can be used to identify many gene families in agronomically important crop species where they are as yet undiscovered.
authors.affiliation: Biological Sciences Department, Missouri S&T, Rolla, MO 65409, USA. rfrank@mst.edu
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Frank
authors.forename: Cyriac
authors.initials: C
authors.lastname: Kandoth
authors.forename: Fikret
authors.initials: F
authors.lastname: Ercal
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D020224:Expressed Sequence Tags; D018745:Genome, Plant / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics*; D020543:Proteome / Q000235:genetics*; D012641:Selection, Genetic
chemical_list: D029681:Arabidopsis Proteins; D020543:Proteome
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