title: Functional expression of transient receptor potential vanilloid-related channels in chronically hypoxic human pulmonary arterial smooth muscle cells.
abstract: Transient receptor potential vanilloid (TRPV) channels are nonselective cation channels pertinent to diverse physiological functions. Multiple TRPV channel subtypes have been identified in different tissues and cloned. The aim of this study was to investigate the role of TRPV channels in hypoxia-induced proliferation of human pulmonary artery smooth muscle cells (PASMCs) and its possible signal pathway. Reverse transcriptase-polymerase chain reaction, real-time polymerase chain reaction, and Western blot analysis were used to detect the expression of TRPV in human PASMCs. Cell number was determined with a hemocytometer. Cytosolic Ca2+ concentration ([Ca2+]cyt) was measured with a dynamic digital Ca2+ imaging system. The mRNA of TRPV1-4 was detected in human PASMCs and chronic hypoxia up-regulated expression levels of the TRPV1 gene and protein. The ability to proliferate, the resting [Ca2+]cyt, and cyclopiazonic acid-induced capacitative Ca2+ entry in human PASMCs were enhanced significantly by chronic hypoxia compared with the control, and these effects were inhibited in a dose-dependent manner by capsazepine, a TRPV1 channel inhibitor. These results suggest that TRPV1 may be a critical pathway or mediator in chronic hypoxia-induced proliferation of human PASMCs.
authors.affiliation: Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, People's Republic of China.
authors.forename: Yue Xiu
authors.initials: YX
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Lu Ping
authors.initials: LP
authors.lastname: Shi
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D002908:Chronic Disease; D006801:Humans; D000860:Hypoxia / Q000378:metabolism* / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D011651:Pulmonary Artery / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D050916:TRPV Cation Channels / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human
references.citation: Circ Res. 2003 Oct 31;93(9):829-38
references.pmid: 14512441
references.citation: Microcirculation. 2007 Nov-Dec;14(8):767-78
references.pmid: 17907014
references.citation: Atherosclerosis. 2007 Dec;195(2):287-96
references.pmid: 17289052
references.citation: FASEB J. 2007 Sep;21(11):2970-9
references.pmid: 17470567
references.citation: Circ Res. 2006 Jul 21;99(2):119-31
references.pmid: 16857972
references.citation: Am J Physiol Lung Cell Mol Physiol. 2003 Dec;285(6):L1233-45
references.pmid: 12909593
references.citation: Circ Res. 2005 Dec 9;97(12):1270-9
references.pmid: 16269659
references.citation: Biochim Biophys Acta. 2007 Aug;1772(8):915-27
references.pmid: 17346945
references.citation: Br J Pharmacol. 1992 Oct;107(2):544-52
references.pmid: 1422598
references.citation: Biochem Biophys Res Commun. 2006 Jun 23;345(1):467-73
references.pmid: 16684507
references.citation: News Physiol Sci. 2004 Apr;19:44-50
references.pmid: 15016901
references.citation: Am J Physiol Lung Cell Mol Physiol. 2007 May;292(5):L1202-10
references.pmid: 17189322
references.citation: Am J Respir Crit Care Med. 2004 Nov 15;170(10):1101-7
references.pmid: 15317671
references.citation: Subcell Biochem. 2007;45:253-71
references.pmid: 18193640
references.citation: Biochim Biophys Acta. 2007 Aug;1772(8):895-906
references.pmid: 17399958
references.citation: Nat Rev Neurosci. 2001 Jun;2(6):387-96
references.pmid: 11389472
references.citation: Am J Physiol. 1999 Aug;277(2):C343-9
references.pmid: 10444411
references.citation: Cell Calcium. 2006 Sep;40(3):261-75
references.pmid: 16806461
references.citation: Circ Res. 2006 Jun 23;98(12):1528-37
references.pmid: 16709899
references.citation: Pulm Pharmacol Ther. 2008;21(1):125-33
references.pmid: 17374498
references.citation: Cell Calcium. 2007 Jul;42(1):17-25
references.pmid: 17184838
references.citation: Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13861-6
references.pmid: 15358862
references.citation: Am J Physiol Lung Cell Mol Physiol. 2004 Nov;287(5):L962-9
references.pmid: 15220115
references.citation: Am J Physiol Lung Cell Mol Physiol. 2006 Jun;290(6):L1267-76
references.pmid: 16399784
references.citation: Microcirculation. 2006 Dec;13(8):671-92
references.pmid: 17085427
references.citation: Circ Res. 2004 Sep 3;95(5):496-505
references.pmid: 15256480