title: Laboratory maintenance of Brucella abortus.
abstract: This unit provides protocols for growth of Brucella abortus on solid or in liquid media and for long-term storage of laboratory stocks. Two issues affecting the culture and storage of isolates of this slow-growing bacterium are emphasized: contamination of cultures and outgrowth of attenuated variants lacking a complete lipopolysaccharide. Laboratories planning to work with B. abortus should be aware that Biosafety Level 3 facilities are required. Furthermore, this organism is classified in the U.S. as a Select Agent, which restricts its use to laboratories registered with the U.S. government's Select Agent programs.
authors.affiliation: University of California at Davis, Davis, California, USA.
authors.forename: Yao-Hui
authors.initials: YH
authors.lastname: Sun
authors.forename: Andreas B
authors.initials: AB
authors.lastname: den Hartigh
authors.forename: Renée M
authors.initials: RM
authors.lastname: Tsolis
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Ficht
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002003:Brucella abortus / Q000166:cytology / Q000254:growth & development* / Q000302:isolation & purification; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D003470:Culture Media