New insights into intra- and intermolecular interactions of immunoglobulins: crystal structure of mouse IgG2b-Fc at 2.1-A resolution.
The structure of the Fc fragment of monoclonal antibody IgG2b from hybridom M75 of Mus musculus has been determined by single crystal X-ray diffraction. This is the first report of the structure of the murine immunoglobulin isotype IgG2b. The structure refined at 2.1 A resolution provides more detailed structural information about native oligosaccharides than was previously available. High-quality Fourier maps provide a clear identification of alpha-l-fucose with partial occupancy in the first branch of the antennary oligosaccharides. A unique Fc:Fc interaction was observed at the C(H)2-C(H)3 interface.
Kolenko
Petr
P
Department of Structure Analysis, Institute of Macromolecular Chemistry AS CR, Praha, Czech Republic. hasek@imc.cas.cz
Dohnálek
Jan
J
Dusková
Jarmila
J
Skálová
Tereza
T
Collard
Renata
R
Hasek
Jindrich
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000936:Antigen-Antibody Complex / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D006031:Glycosylation; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D051379:Mice; D009844:Oligosaccharides / Q000737:chemistry; D017433:Protein Structure, Secondary
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D009844:Oligosaccharides
Mol Cell. 2001 Apr;7(4):867-77
11336709
J Immunol. 2005 Nov 15;175(10):6909-14
16272350
Proteins. 2003 Feb 15;50(3):437-50
12557186
Biochemistry. 1997 Feb 18;36(7):1581-97
9048542
J Mol Biol. 2003 Jan 31;325(5):979-89
12527303
Immunology. 1979 Nov;38(3):631-40
521055
Kidney Int. 1989 Apr;35(4):993-1003
2651776
Talanta. 2001 Oct 31;55(4):821-9
18968430
Biopolymers. 1983 Dec;22(12):2577-637
6667333
Biochemistry. 1981 Apr 28;20(9):2361-70
7236608
Clin Dev Immunol. 2004 Mar;11(1):61-6
15154614
Carbohydr Res. 2004 Apr 2;339(5):1015-20
15010309
J Immunol. 2000 Apr 15;164(8):4178-84
10754313
Nat Struct Biol. 1999 May;6(5):458-63
10331874
J Immunol Methods. 2001 Jul 1;253(1-2):13-21
11384665
Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):760-3
15299374
J Mol Biol. 2007 May 4;368(3):767-79
17368483
J Struct Biol. 1999 Apr-May;125(2-3):156-65
10222271
J Mol Biol. 2002 May 24;319(1):9-18
12051932
Immunology. 1979 Nov;38(3):641-8
521056
Immunology. 1983 Mar;48(3):469-76
6826201
Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
15299926
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):191-205
10089410
Immunology. 1983 Mar;48(3):477-88
6186597
Methods Enzymol. 1997;276:581-594
27799116
Nucleic Acids Res. 2000 Jan 1;28(1):235-42
10592235
Methods Enzymol. 1997;276:307-26
27754618
Nature. 2000 Jul 20;406(6793):267-73
10917521
Proc Natl Acad Sci U S A. 1976 Mar;73(3):915-8
1062805
Science. 2000 Feb 18;287(5456):1279-83
10678837
Structure. 1995 Mar 15;3(3):265-78
7788293
FEBS Lett. 2000 May 19;473(3):349-57
10818239
Virology. 1992 Apr;187(2):620-6
1312272
BMC Bioinformatics. 2004 Jun 04;5:69
15180909
Science. 2006 Aug 4;313(5787):670-3
16888140