A preliminary study of direct application of atelocollagen into a wound lesion in the dog cornea.
OBJECTIVE
To evaluate the effectiveness of atelocollagen for canine corneal wound healing.


METHODS
Atelocollagen was used to fill a transplant bed in the central cornea, which was then covered with a contact lens. The wound healing process was analyzed clinically, morphologically, and biochemically.


RESULTS
At the early healing stage, both the pupillary zone and details of the iris were observed. The stromal collagen fibrils normalized in a time-dependent manner. Type III collagen in the wound area was detected faintly throughout the experimental period.


CONCLUSIONS
This novel method is advantageous for accelerating wound healing without causing inflammation.
Nagayasu
Aya
A
Department of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
Hosaka
Yoshinao
Y
Yamasaki
Ayako
A
Tsuzuki
Keiko
K
Ueda
Hiromi
H
Honda
Tomoaki
T
Takehana
Kazushige
K
D000818:Animals; D002452:Cell Count; D003094:Collagen / Q000008:administration & dosage*; D024061:Collagen Type III / Q000378:metabolism; D003261:Contact Lenses; D003315:Cornea / Q000378:metabolism; D065306:Corneal Injuries; D003319:Corneal Stroma / Q000378:metabolism / Q000648:ultrastructure; D004195:Disease Models, Animal; D004285:Dogs; D004337:Drug Carriers / Q000008:administration & dosage*; D019573:Epithelium, Corneal / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D008297:Male; D046529:Microscopy, Electron, Transmission; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
D024061:Collagen Type III; D004337:Drug Carriers; C059821:atelocollagen; D003094:Collagen