title: Laboratory maintenance of Treponema denticola.
abstract: This unit describes the methods, media, and equipment necessary for routine laboratory culture and handling of the anaerobic oral spirochete Treponema denticola. Topics discussed include nutrient requirements, recommended media formulations, and expected growth kinetics, as well as methods and equipment necessary to maintain anaerobic conditions. An additional protocol on isolation of T. denticola from clinical samples is included.
authors.affiliation: University of Michigan School of Dentistry, Ann Arbor, Michigan, USA.
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Fenno
mesh_terms: D000332:Aerobiosis; D001431:Bacteriological Techniques / Q000379:methods*; D003264:Containment of Biohazards; D003470:Culture Media / Q000737:chemistry*; D004864:Equipment and Supplies; D006801:Humans; D008853:Microscopy; D013194:Staining and Labeling; D048373:Treponema denticola / Q000166:cytology / Q000254:growth & development* / Q000302:isolation & purification; D014211:Treponemal Infections / Q000382:microbiology
chemical_list: D003470:Culture Media