[Analysis of features of 15 successful positional cloning of QTL in rice].
As the most efficient strategy in gene clone, positional cloning has been used widely in QTL cloning in rice. The objective of this paper is to make summary features of QTL positional cloning based on 15 successful positional cloning attempts. (1) most of the populations used in the analysis were derived from interspecific or intersubspecies; (2) the target QTL had been identified with very large phenotypic effects; (3) the candidate genomic region was usually narrowed down to 40 kb; (4) the primary mapping result was exact; and the fine mapping population was more than 6,000, while the number of recessive population was more than 1,500. Otherwise, the nodus of QTL positional cloning and the corresponding solving methods were discussed.
Jiang
Shu-Kun
SK
Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture Key Laboratory of Northern Japonica Rice Breeding of Liaoning Province, Shenyang Agricultural University, Shenyang 110161, China. sk_jiang@163.com
Xu
Zheng-Jin
ZJ
Chen
Wen-Fu
WF
D018745:Genome, Plant / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D040641:Quantitative Trait Loci