Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans.
Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.
Wiederhold
Nathan P
NP
University of Texas at Austin College of Pharmacy, Austin, Texas, USA. wiederholdn@uthscsa.edu
Grabinski
Jodi L
JL
Garcia-Effron
Guillermo
G
Perlin
David S
DS
Lee
Samuel A
SA
D000077612:Anidulafungin; D000935:Antifungal Agents / Q000494:pharmacology*; D001483:Base Sequence; D002176:Candida albicans / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology; D000077336:Caspofungin; D004252:DNA Mutational Analysis / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics; D054714:Echinocandins / Q000494:pharmacology*; D005800:Genes, Fungal; D005964:Glucosyltransferases / Q000235:genetics*; D006801:Humans; D055666:Lipopeptides / Q000494:pharmacology; D000077551:Micafungin; D017354:Point Mutation
D000935:Antifungal Agents; D004271:DNA, Fungal; D054714:Echinocandins; D055666:Lipopeptides; D000077612:Anidulafungin; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase; D000077336:Caspofungin; D000077551:Micafungin
Pharmacotherapy. 2006 Jun;26(6):877-80
16716141
Antimicrob Agents Chemother. 2007 Nov;51(11):4214-6
17785512
N Engl J Med. 2007 Jun 14;356(24):2472-82
17568028
Antimicrob Agents Chemother. 2004 Apr;48(4):1382-3
15047549
Antimicrob Agents Chemother. 2007 May;51(5):1876-8
17325225
Antimicrob Agents Chemother. 2005 Aug;49(8):3264-73
16048935
Antimicrob Agents Chemother. 2005 Feb;49(2):767-9
15673762
J Clin Microbiol. 2005 Feb;43(2):906-8
15695702
J Clin Microbiol. 2008 Aug;46(8):2620-9
18579718
Antimicrob Agents Chemother. 2007 Jun;51(6):2253-6
17420211
Lancet. 2007 May 5;369(9572):1519-1527
17482982
N Engl J Med. 2002 Dec 19;347(25):2020-9
12490683
Clin Infect Dis. 2006 Apr 1;42(7):938-44
16511756
Antimicrob Agents Chemother. 2006 Jun;50(6):2058-63
16723566
Electrophoresis. 2006 Aug;27(15):3042-7
16800029
Clin Infect Dis. 2007 Oct 1;45(7):883-93
17806055
J Clin Microbiol. 2004 Oct;42(10):4788-95
15472342
Med Mycol. 2005 Sep;43(6):559-64
16320498
Breast Cancer Res Treat. 2006 Jan;95(1):13-6
16317586
Antimicrob Agents Chemother. 2006 Jul;50(7):2522-4
16801435
Clin Chim Acta. 2006 Jan;363(1-2):83-94
16165119
Antimicrob Agents Chemother. 2007 May;51(5):1616-20
17307976