[Effects of Chaiqinchengqi decoction on exocrine function of pancreatic acinar cells of acute pancreatitis rats].
OBJECTIVE
To investigate the effects of Chaiqinchengqi decoction(CQCQ-D) on exocrine function of pancreatic acinar cells and the changes of intracellular calcium concentration ([Ca2+]i) of acute pancreatitis (AP) rats, so as to explore the mechanism of CQCQ-D.


METHODS
Acute pancreatitis model was induced in Sprague-Dawley rat by ligation of bile-pancreatic duct, pancreatic acinar cells were isolated by collagenase digestion and in vitro co-incubated with the drug serum containing Chaiqingchengqi decoction(CQCQ-S) which was collected from normal rats intragastrically fed on CQCQ-D, then the amylase activity secreted into the buffer was assayed and intracellular fluorescent intensity were observed and analyzed to investigate the changes of intracellular calcium concentration ([Ca2+]i).


RESULTS
Amylase secretion in AP group was lower than that in sham-operation(SO) group (P < 0.01), while CQCQ-S treatment led to more significant decrease (P < 0.05); [Ca2+]i elevated in AP rats (P < 0.05), while the level of [Ca2+]i elevation was reduced after CQCQ-S treatment (P < 0.05).


CONCLUSIONS
CQCQ-D has inhibitive effects on exocrine function of pancreatic acinar cells and the calcium overload of AP rats.
Deng
Li-hui
LH
Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Yang
Xiao-nan
XN
Huang
Lei
L
Xiang
Da-kai
DK
Xia
Qing
Q
D000208:Acute Disease; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D008297:Male; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D046790:Pancreas, Exocrine / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D010195:Pancreatitis / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley
C528108:Chai Qin Cheng Qi decoction; D004365:Drugs, Chinese Herbal; D002118:Calcium