Morphogenesis of liposomes caused by polycation-induced actin assembly formation.
We investigated the effects of polycation-mediated actin assembly on the morphological transformation of the lipid vesicle membrane by spatiotemporally controlling actin assembly. By triggering the radical polymerization of the cationic monomer using UV irradiation, we achieved a varied photoinduced assembly of actin in bulk solution. Furthermore, we designed liposomes containing actin and cationic monomers. In these actin-encapsulated liposomes, various actin assemblies were formed by UV irradiation similar to that observed in bulk solution. Moreover, morphogenesis of actin-encapsulated liposomes was observed in liposomes encapsulated with G-actin but not with F-actin. This result indicates that a dynamic polymerization of G-actin is important for vesicle protrusion.
Maemichi
Haruka
H
Department of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
Shikinaka
Kazuhiro
K
Kakugo
Akira
A
Furukawa
Hidemitsu
H
Osada
Yoshihito
Y
Gong
Jian Ping
JP
D008841:Actin Cytoskeleton / Q000737:chemistry; D000199:Actins / Q000737:chemistry*; D000818:Animals; D002412:Cations; D008027:Light; D008081:Liposomes / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D009113:Muramidase / Q000737:chemistry; D049897:Pectinidae; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D013050:Spectrometry, Fluorescence / Q000379:methods; D014466:Ultraviolet Rays
D000199:Actins; D002412:Cations; D008081:Liposomes; D009113:Muramidase; D011073:Polyamines; D000071228:Polyelectrolytes; C421864:hen egg lysozyme