Photochromic nucleobase: reversible photoisomerization, photochemical properties and photoregulation of hybridization.
We have developed a set of photochromic nucleobases (PCNs), which reversibly change the photochemical and physical properties such as fluorescence intensity upon cis-trans photoisomerization by the external light stimuli. PCNs showed very rapid and efficient reversible photoisomerization by illumination at specific wavelength. In addition, photoisomerization can be iteratively performed by alternate illumination with 250 approximately 350 nm and 350 approximately 500 nm light without any side reactions. We demonstrated a new type of the photoregulation of hybridization using this switching property.
Ogasawara
Shinzi
S
Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. o_shinji@riken.jp
Maeda
Mizuo
M
D005456:Fluorescent Dyes / Q000737:chemistry* / Q000528:radiation effects; D007536:Isomerism; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010777:Photochemistry; D011687:Purines / Q000737:chemistry* / Q000528:radiation effects; D011743:Pyrimidines / Q000737:chemistry* / Q000528:radiation effects
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011687:Purines; D011743:Pyrimidines