title: Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake.
abstract: OBJECTIVE
  To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland.
  
  
  METHODS
  Isolates presenting MICs of nalidixic acid or ciprofloxacin > or = 1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids were transferred by transformation, and further analysis of the genetic structures surrounding the qnrS2 gene was carried out by PCR and sequencing.
  
  
  RESULTS
  A qnrS2 gene was identified from a single Aeromonas allosaccharophila isolate (Lugano lake, Lugano), as part of a mobile insertion cassette located on a broad host range IncU-type plasmid. This plasmid co-harboured a class 1 integron containing the aac(6')-Ib-cr, bla(OXA-1), catB3 and arr-3 gene cassettes.
  
  
  CONCLUSIONS
  These findings strengthen further the role of Aeromonas spp. as a reservoir of antimicrobial resistance determinants in the environment.
authors.affiliation: Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France.
authors.forename: Renata Cristina
authors.initials: RC
authors.lastname: Picão
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Antonella
authors.initials: A
authors.lastname: Demarta
authors.forename: Carla Sofia Ferreira
authors.initials: CS
authors.lastname: Silva
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Corvaglia
authors.forename: Orlando
authors.initials: O
authors.lastname: Petrini
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000333:Aeromonas / Q000187:drug effects* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005618:Fresh Water / Q000382:microbiology*; D023061:Gene Order; D005798:Genes, Bacterial; D032023:Integrons; D020071:Interspersed Repetitive Sequences; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D013557:Switzerland
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015363:Quinolones