title: The level of sulfane sulfur in the fungus Aspergillus nidulans wild type and mutant strains.
abstract: The interdependence of the sulfane sulfur metabolism and sulfur amino acid metabolism was studied in the fungus Aspergillus nidulans wild type strain and in mutants impaired in genes encoding enzymes involved in the synthesis of cysteine (a precursor of sulfane sulfur) or in regulatory genes of the sulfur metabolite repression system. It was found that a low concentration of cellular cysteine leads to elevation of two sulfane sulfurtransferases, rhodanase and cystathionine gamma-lyase, while the level of 3-mercaptopyruvate sulfurtransferase remains largely unaffected. In spite of drastic differences in the levels of biosynthetic enzymes and of sulfur amino acids due to mutations or sulfur supplementation of cultures, the level of total sulfane sulfur is fairly stable. This stability confirms the crucial role of sulfane sulfur as a fine-tuning regulator of cellular metabolism.
authors.affiliation: Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kopernika 7, 31-034, Cracow, Poland. mbwrobel@cyf-kr.edu.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Wróbel
authors.forename: Irmina
authors.initials: I
authors.lastname: Lewandowska
authors.forename: Patrycja
authors.initials: P
authors.lastname: Bronowicka-Adamska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Paszewski
mesh_terms: D000603:Amino Acids, Sulfur / Q000096:biosynthesis / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*; D013466:Sulfurtransferases / Q000378:metabolism*
chemical_list: D000603:Amino Acids, Sulfur; D013455:Sulfur; D013466:Sulfurtransferases; C063506:sulfane sulfurtransferase