[Analysis on the characters of progenies of the rice transformed with anti-sense waxy gene].
The segregation of exogenous genes was studied by hygromycin-resistant and PCR experiments in the transgenic rice (Oryza sativa L. subsp. japonica and indica) with anti-sense waxy gene, meanwhile the change of amylose and waxy protein contents in progenies of transgenic rice was analyzed. The results showed no matter the rice Guangjing No.1 (O. Sativa L. subsp. japonica) were transformed by p13w4 plasmid carrying anti-sense waxy gene and hygromycin-resistant gene, or in the rice 01Z5202 (O. sativa L. subsp. indica) were co-transformed by p13w8 plasmid carrying anti-sense waxy gene and p1300 plasmid carrying hygromycin-resistant gene, the target gene(s) had been segregated in the progenies; the content of amylose of the transgenic plants was lower than those in non-transgenic ones, and the content of amylose in some of transgenic plants was less than 10.0% (of the weight of grain), which was much lower than those in the control (about 22.04%); and the analysis with SDS-PAGE showed the content of the waxy protein are positively correlated with the con-tent of amylose in the tested transgenic rice materials.
Liu
Qing
Q
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China. lqing2003@163.com
Wu
Shun
S
Shen
Ge-Zhi
GZ
Peng
Qiong
Q
Liu
Tao-Li
TL
Cheng
Peng
P
Xiao
Lang-Tao
LT
D000688:Amylose / Q000378:metabolism*; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D016372:RNA, Antisense / Q000235:genetics / Q000494:pharmacology*; D013214:Starch Synthase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D019076:Transgenes
D010940:Plant Proteins; D016372:RNA, Antisense; C060756:waxy protein, plant; D000688:Amylose; D013214:Starch Synthase