title: Association of MMP-3 5A/6A gene polymorphism with susceptibility to carotid atherosclerosis.
abstract: OBJECTIVE
  Stromelysin-1 (MMP-3) as a key member of metalloproteinase family could have an important role in atherogenesis. The 5A/6A polymorphism in the promoter of MMP-3 gene affects the level of MMP-3 gene expression. We assessed whether the MMP-3 promoter low- and high-activity genotypes are related to susceptibility for carotid atherosclerosis (CA) in Serbian population.
  
  
  METHODS
  The study group of case-control design consisted of 515 participants. The 265 patients with ultrasonographic evidence of carotid plaque presence were recruited for the study. The 5A/6A polymorphism was typed by RFLP-PCR.
  
  
  RESULTS
  There was significantly higher prevalence of genotypes containing 6A allele in the patients with CA compared to controls (p<0.05). The model of inheritance with the dominant effect of 6A allele gave elevated and significant OR for carotid atherosclerosis (adjusted OR 2.35, CI=1.0-5.5, p=0.048).
  
  
  CONCLUSIONS
  Subjects carrying genotypes with 6A allele had significantly higher susceptibility to carotid atherosclerosis.
authors.affiliation: VINCA Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, P.O. Box 522, 11001 Belgrade, Serbia. tamariska@vin.bg.ac.yu
authors.forename: Tamara
authors.initials: T
authors.lastname: Djurić
authors.forename: Maja
authors.initials: M
authors.lastname: Zivković
authors.forename: Djordje
authors.initials: D
authors.lastname: Radak
authors.forename: Djole
authors.initials: D
authors.lastname: Jekić
authors.forename: Sandra
authors.initials: S
authors.lastname: Radak
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Stojković
authors.forename: Ranko
authors.initials: R
authors.lastname: Raicević
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Stanković
authors.forename: Dragan
authors.initials: D
authors.lastname: Alavantić
mesh_terms: D000328:Adult; D002340:Carotid Artery Diseases / Q000201:enzymology* / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3