Fabrication of cytochrome c-poly(5-amino-2-napthalenesulfonic acid) electrode by one step procedure and direct electrochemistry of cytochrome c.
Herein, we reported for the first time one step procedure for the preparation of cytochrome c (cyt c)-poly (5-amino-2-napthalenesulfonic acid) (PANS) modified glassy carbon electrode by cyclic voltammetrically (CV). Hereafter, we called the above modified electrode as cyt c-PANS electrode. The presence of cyt c on modified electrode was investigated with electrochemical quartz crystal microbalance (EQCM), CV, and superoxide radicals reaction studies. The reaction between cyt c in the modified electrode and superoxide radicals in solution, was exemplified by cyclic voltammetric measurements. Surface morphology of the modified electrode was investigated by using atomic force microscopy (AFM). The modified electrode showed a pair of well defined redox peak in PBS solution, pH 6.7. The modified electrode utilized for electrocatalytic reduction as well as amperometric determination of hydrogen peroxide (H(2)O(2)). The detection limit and linear range for H(2)O(2) were 5 and 50 microM to 7 mM, respectively.
Balamurugan
A
A
Electroanalysis and Bioelectrochemistry lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei, Taiwan 106, ROC.
Chen
Shen-Ming
SM
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D045304:Cytochromes c / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006861:Hydrogen Peroxide / Q000032:analysis*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D009579:Nitrobenzoates / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D009579:Nitrobenzoates; C019251:5-amino-2-nitrobenzoic acid; D045304:Cytochromes c; D006861:Hydrogen Peroxide