title: Isolation, characterisation and osteogenic potential of human bone marrow stromal cells derived from the medullary cavity of the femur.
abstract: Marrow stromal cells (MSC) are increasingly being introduced in orthopaedic practice as potentially powerful effectors of bone regeneration. Since cell recovery of MSC is affected by a high degree of individual variability, sources for collecting adequate amounts of safe and effective MSC under routine conditions are needed. We analysed if femoral bone marrow, which is usually discarded during total hip arthroplasty procedures, is a reliable source of MSC to enhance bone healing and regeneration. Mononuclear cells were isolated, assayed for typical MSC markers, harvested under appropriate culture conditions and evaluated for their ability to differentiate into osteoblasts. Cell recovery and osteogenic potential were independent from donor gender or age, suggesting that elderly individuals are eligible for autologous cell therapy. Although heterogeneous, the pool of MSC recovered from femoral marrow without further in vitro selection or manipulation proved highly effective in proliferating and differentiating along the osteogenic lineage. In conclusion, this source of MSC offers a valuable tool to be used to promote osteogenesis and implant fixation.
authors.affiliation: Laboratorio di Fisiopatologia degli Impianti Ortopedici, Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136, Bologna, Italy. elisa.leonardi@ior.it
authors.forename: Elisa
authors.initials: E
authors.lastname: Leonardi
authors.forename: Valentina
authors.initials: V
authors.lastname: Devescovi
authors.forename: Francesca
authors.initials: F
authors.lastname: Perut
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ciapetti
authors.forename: Armando
authors.initials: A
authors.lastname: Giunti
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D019644:Arthroplasty, Replacement, Hip; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology*; D001861:Bone Regeneration / Q000502:physiology; D002448:Cell Adhesion; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D002478:Cells, Cultured / Q000737:chemistry / Q000166:cytology; D005260:Female; D005269:Femur / Q000166:cytology*; D006618:Hip Dislocation, Congenital / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D015207:Osteoarthritis, Hip / Q000601:surgery; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D012737:Sex Factors; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering; D055815:Young Adult
chemical_list: D015415:Biomarkers
references.citation: J Bone Joint Surg Am. 2008 Feb;90 Suppl 1:111-9
references.pmid: 18292365
references.citation: Int J Biochem Cell Biol. 2004 Apr;36(4):568-84
references.pmid: 15010324
references.citation: Transplantation. 2007 Apr 27;83(8):1019-26
references.pmid: 17452890
references.citation: Arthritis Rheum. 2008 Jun;58(6):1731-40
references.pmid: 18512779
references.citation: Clin Orthop Relat Res. 2002 Feb;(395):66-80
references.pmid: 11937867
references.citation: Int J Biochem Cell Biol. 2004 Apr;36(4):585-97
references.pmid: 15010325
references.citation: Tissue Eng Part C Methods. 2008 Sep;14(3):185-96
references.pmid: 18620484
references.citation: Cytotherapy. 2007;9(4):328-37
references.pmid: 17573608
references.citation: Int J Cancer. 2004 Oct 10;111(6):829-38
references.pmid: 15300794
references.citation: N Engl J Med. 2005 May 12;352(19):1959-66
references.pmid: 15888696
references.citation: Arthritis Rheum. 2002 Mar;46(3):704-13
references.pmid: 11920406
references.citation: Clin Orthop Relat Res. 2005 Mar;(432):242-51
references.pmid: 15738828
references.citation: Biomaterials. 2000 Dec;21(23):2347-59
references.pmid: 11055282
references.citation: Rheumatology (Oxford). 2008 Feb;47(2):126-31
references.pmid: 17986482
references.citation: Clin Orthop Relat Res. 2007 Dec;465:202-7
references.pmid: 17891036
references.citation: Tissue Eng. 2001 Apr;7(2):211-28
references.pmid: 11304456
references.citation: Biomaterials. 2004 Feb;25(5):757-68
references.pmid: 14609664
references.citation: J Mater Sci Mater Med. 2004 Oct;15(10):1123-8
references.pmid: 15516873
references.citation: Ortop Traumatol Rehabil. 2007 Jan-Feb;9(1):63-7
references.pmid: 17514176
references.citation: PLoS One. 2008 May 21;3(5):e2213
references.pmid: 18493317
references.citation: Biochem Biophys Res Commun. 2003 Nov 28;311(4):1008-13
references.pmid: 14623282
references.citation: J Mater Sci Mater Med. 2007 Jun;18(6):1225-37
references.pmid: 17277983
references.citation: J Cell Physiol. 2007 Nov;213(2):341-7
references.pmid: 17620285
references.citation: Stem Cells. 2007 Dec;25(12):3244-51
references.pmid: 17872501
references.citation: J Bone Joint Surg Am. 2005 Jul;87(7):1430-7
references.pmid: 15995108
references.citation: Cytotherapy. 2006;8(4):315-7
references.pmid: 16923606
references.citation: Semin Cell Dev Biol. 2007 Dec;18(6):846-58
references.pmid: 18024097
references.citation: Cell Cycle. 2007 Dec 1;6(23):2884-9
references.pmid: 18000405
references.citation: J Cell Biol. 2001 May 28;153(5):1133-40
references.pmid: 11381097
references.citation: Stem Cell Rev. 2007 Dec;3(4):239-48
references.pmid: 18074246
references.citation: J Cell Physiol. 2004 Jan;198(1):110-8
references.pmid: 14584050
references.citation: Cytotherapy. 2008;10(5):460-8
references.pmid: 18608349
references.citation: Biomaterials. 2006 Dec;27(36):6150-60
references.pmid: 16965811
references.citation: J Orthop Res. 2008 Jul;26(7):910-7
references.pmid: 18302252
references.citation: J Orthop Res. 2001 Jan;19(1):117-25
references.pmid: 11332607
references.citation: Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8932-7
references.pmid: 12084934
references.citation: Aging Cell. 2008 Jun;7(3):335-43
references.pmid: 18248663
references.citation: Tissue Eng. 2005 May-Jun;11(5-6):663-73
references.pmid: 15998208
references.citation: Expert Opin Biol Ther. 2008 Mar;8(3):255-68
references.pmid: 18294098
references.citation: Tissue Eng. 2007 Apr;13(4):819-29
references.pmid: 17371154
references.citation: Calcif Tissue Int. 2002 Jul;71(1):36-44
references.pmid: 12200657
references.citation: Cryobiology. 2008 Jun;56(3):209-15
references.pmid: 18430412
references.citation: Exp Gerontol. 2005 Dec;40(12):926-30
references.pmid: 16125890