title: Toxicity and interaction of titanium dioxide nanoparticles with microtubule protein.
abstract: Titanium dioxide (TiO2) nanoparticles (NPs) are widely used in several manufactured products. The small size of NPs facilitates their uptake into cells as well as transcytosis across epithelial cells into blood and lymph circulation to reach different sites, such as the central nervous system. Different studies have shown the risks that TiO2 NPs in the neuronal system and other organs present. As membrane-bound layer aggregates or single particles, TiO2 NPs can enter not only cells, but also mitochondria and nuclei. Therefore these particles can interact with cytoplasmic proteins such as microtubules (MTs). MTs are cytoskeletal proteins that are essential in eukaryotic cells for a variety of functions, such as cellular transport, cell motility and mitosis. MTs in neurons are used to transport substances such as neurotransmitters. Single TiO2 NPs in cytoplasm can interact with these proteins and affect their crucial functions in different tissues. In this study, we showed the effects of TiO2 NPs on MT polymerization and structure using ultraviolet spectrophotometer and fluorometry. The fluorescent spectroscopy showed a significant tubulin conformational change in the presence of TiO2 NPs and the ultraviolet spectroscopy results showed that TiO2 NPs affect tubulin polymerization and decrease it. The aim of this study was to find the potential risks that TiO2 NPs pose to human organs and cells.
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Biochemistry, University of Tehran, Tehran 13145-1384, Iran.
authors.forename: Zahra Naghdi
authors.initials: ZN
authors.lastname: Gheshlaghi
authors.forename: Gholam Hossein
authors.initials: GH
authors.lastname: Riazi
authors.forename: Shahin
authors.initials: S
authors.lastname: Ahmadian
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Ghafari
authors.forename: Roya
authors.initials: R
authors.lastname: Mahinpour
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D005470:Fluorometry; D008868:Microtubule Proteins / Q000737:chemistry / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D009391:Nephelometry and Turbidimetry; D011487:Protein Conformation; D012756:Sheep; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014025:Titanium / Q000737:chemistry*; D014404:Tubulin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D008868:Microtubule Proteins; D014404:Tubulin; C009495:titanium dioxide; D014025:Titanium