ERK-dependent PSD-95 induction in the gustatory cortex is necessary for taste learning, but not retrieval.
The processes underlying long-term memory formation in the neocortex are poorly understood. Using taste learning, we found learning-related induction of PSD-95 in the gustatory cortex, which was temporally restricted, coupled to the learning of a novel, but not familiar, taste and controlled by ERK. Using temporally and spatially restricted RNA interference knockdown of PSD-95 in vivo, we found that PSD-95 induction is necessary for learning novel tastes, but not for the recollection of familiar ones.
Elkobi
Alina
A
Center for Brain and Behavior, Department of Neurobiology and Ethology, University of Haifa, Haifa 30905, Israel.
Ehrlich
Ingrid
I
Belelovsky
Katya
K
Barki-Harrington
Liza
L
Rosenblum
Kobi
K
D000704:Analysis of Variance; D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D001362:Avoidance Learning / Q000502:physiology*; D001522:Behavior, Animal; D003216:Conditioning, Operant / Q000187:drug effects / Q000502:physiology; D000076122:Disks Large Homolog 4 Protein; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology; D066298:In Vitro Techniques; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D034622:RNA Interference / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013003:Somatosensory Cortex / Q000187:drug effects / Q000502:physiology*; D013649:Taste / Q000502:physiology*; D014162:Transfection
D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011500:Protein Synthesis Inhibitors; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D000841:Anisomycin; D048049:Extracellular Signal-Regulated MAP Kinases