title: c-Lysozyme from Senegalese sole (Solea senegalensis): cDNA cloning and expression pattern.
abstract: Lysozymes are key molecules of innate immunity and proved high bactericidal activity in fish, thus becoming attractive as tools for enhancing fish defences. In this study, a full-length c-type lysozyme cDNA from Senegalese sole (Solea senegalensis) has been cloned and characterized. The cDNA sequence was inferred from two overlapping fragments obtained by RACE-PCR and consisting on 631bp coding for 143 aminoacids. Catalytic and other conserved residues required for lysozyme activity were identified. Pair wise alignments showed the higher identities with c-type lysozyme from other flatfish. Expression patterns under various conditions showed a basal level and a clear upregulation mostly in hematopoietic organs after stimulation with LPS or infection with Photobacterium damselae. This study represents a first step on the genetics and function of the c-lysozyme of Senegalese sole, though disclosing g-DNA structure, allelic variability and antibacterial activity must be requirements prior its immunological properties might have biotechnological applications.
authors.affiliation: Department of Genetics, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain.
authors.forename: M Alejandra
authors.initials: MA
authors.lastname: Fernández-Trujillo
authors.forename: Javier
authors.initials: J
authors.lastname: Porta
authors.forename: Manuel
authors.initials: M
authors.lastname: Manchado
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Borrego
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Alvarez
authors.forename: Julia
authors.initials: J
authors.lastname: Béjar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D005412:Flatfishes / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D005880:Gills / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics / Q000378:metabolism*; D055747:SOX Transcription Factors / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D013154:Spleen / Q000378:metabolism
chemical_list: C103073:D protein, Drosophila; D018076:DNA, Complementary; D029721:Drosophila Proteins; D055747:SOX Transcription Factors; D009113:Muramidase