[Changes of the Wnt signalling key effectors in brain of rat embryo suffering from intrauterine hypoxia-ischemia brain damage].
OBJECTIVE
To investigate the relationship between Wnt pathway and intrauterine hypoxia-ischemia brain damage.


METHODS
The gestational rats (15-17days) were randomly divided into sham operation group and transient intrauterine ischemia group. For transient intrauterine ischemia group, the gestational rats' bilateral uterine arteries were clamped for 30 min, followed by reperfusion. For sham operation, animals were subjected to the same surgical procedures, except that bilateral uterine arteries were not clamped. Samples were collected at 24, 48, and 72 h after reperfusion. The whole brain tissues were dissected for Nissl stain and Caspase-3 immumohistochemical. The expressions of GSK-3 beta and beta-catenin protein in the two groups were analyzed with Western Blotting.


RESULTS
The Nissl body of the transient intrauterine ischemia group decreased and the masculine cell of Caspase-3 was significantly higher than sham operation group at each time point (P < 0.05). The sham operation group is 12.71 +/- 1.42 (24 h), 11.45 +/- 1.50 (48 h), 14.10 +/- 1.29 (72 h) and the transient intrauterine ischemia group is 26.29 +/- 1.64 (24 h), 32.38 +/- 1.05 (48 h), 39.20 +/- 1.88 (72 h). beta-catenin expression between the two groups was no statistically significant difference. However, expression of GSK-3 beta in transient intrauterine ischemia group increased (P < 0.05). The sham operation group is 1.75 +/- 1.07 (24 h), 1.34 +/- 1.03 (48 h), 1.48 +/- 1.10 (72 h) and the transient intrauterine ischemia group is 3.25 +/- 1.34 (24 h), 2.99 +/- 1.26 (48 h), 3.10 +/- 1.35 (72 h).


CONCLUSIONS
Wnt pathway may play an important part in intrarterine hypoxia-ischemia brain damage.
Tang
Yi-wei
YW
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Luo
Rong
R
Zhou
Hui
H
Liu
Wei
W
Mao
Meng
M
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000196:embryology / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D005260:Female; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D051155:Wnt1 Protein / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism
D051155:Wnt1 Protein; C495245:Wnt1 protein, rat; D051176:beta Catenin; D038362:Glycogen Synthase Kinase 3; D053148:Caspase 3