[Identification of SNPs located in putative microRNA tar-get region of six functional genes in chickens through bioinformatic analysis].
GDF-8, IGF-I, IGF-III, IGF2R, IGFBP2, and GHR are candidate genes affecting important economic trait in chicken. The putative microRNA target sites of 3' untranslated region of these genes were identified by means of specialized algorithms (miRanda and TargetScan), and the candidate SNPs located in the microRNA target region were also identified. Approximately 125 candidate SNPs were found throughout the 26 putative microRNA target regions in six gene 3'-UTR. Among the 125 SNPs, 47 were located in the microRNA targets, 44 and 35 were located in 5'and 3'flanking regions which equalled to the size of the given target. Twelve of the 47 candidates were located in the match of the microRNA seed. These SNPs, which were located in the match of the microRNA seed and 3 ' flanking regions may affect microRNA regulation and contribute to poultry phenotypic variation.
Geng
Li-Ying
LY
Department of Life Science, Hebei Normal University of Science & Technology, Changli 066600, China. rosegengly@126.com
Zhang
Chuan-Sheng
CS
DU
Li-Xin
LX
D000818:Animals; D002645:Chickens; D019295:Computational Biology / Q000379:methods*; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D055435:Myostatin / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D020506:Untranslated Regions / Q000235:genetics
D035683:MicroRNAs; D055435:Myostatin; D020506:Untranslated Regions; D007334:Insulin-Like Growth Factor I