title: Isolation of small RNAs using biotinylated PNAs.
abstract: In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from human total RNA in pure form. Damage to the RNA appears to be negligible by this method because the method is rapid and does not require a high temperature treatment to facilitate RNA-PNA binding.
authors.affiliation: Department of Bioscience and Biotechnology, Okayama University, Tsushimanaka, Okayama, Japan. ohtsuk@cc.okayama-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohtsuki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujimoto
authors.forename: Maya
authors.initials: M
authors.lastname: Kamimukai
authors.forename: Chisato
authors.initials: C
authors.lastname: Kumano
authors.forename: Mizuki
authors.initials: M
authors.lastname: Kitamatsu
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sisido
mesh_terms: D000818:Animals; D001483:Base Sequence; D019921:Biotinylation; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008722:Methods; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020135:Peptide Nucleic Acids / Q000737:chemistry; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020135:Peptide Nucleic Acids; D012329:RNA, Bacterial; D012356:RNA, Transfer, Leu; D012313:RNA