title: Identification of ultraviolet B-sensitive genes in human peripheral blood cells.
abstract: Ultraviolet B (UVB) is a serious irritant for the skin and increases a risk for skin cancer. To identify UVB-sensitive genes in peripheral blood, 11 healthy male volunteers were exposed to 0.3 J/cm(2) of narrow-band (NB)-UVB, about half of minimal erythema dose (MED) in Japanese, and gene expression in blood was analyzed at 4 h, 24 h, 4 d and 7 d after the irradiation using microarray carrying oligonucleotide probes for 2,000 stress-responsive genes. RNA prepared before the irradiation was used as a reference control. Microarray analysis identified 21 genes as UVB-responsive genes with a peak at 24 h in 6 subjects, and real-time PCR validated the significant down-regulation of 9 (ABCB10, ATF1, ABCD3, TANK, FAS, SLC30A9, CHUK, CASP1, and ABCE1) out of the 21 genes in 11 subjects. Considering sensitive and characteristic features of 9 marker genes, they may be useful indicators for monitoring systemic response to UVB irradiation.
authors.affiliation: Departments of Dermatology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Jung
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kawai
authors.forename: Hwan Ki
authors.initials: HK
authors.lastname: Park
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kubo
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Rokutan
authors.forename: Seiji
authors.initials: S
authors.lastname: Arase
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000378:metabolism* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D015536:Down-Regulation / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects*; D020869:Gene Expression Profiling; D005819:Genetic Markers / Q000528:radiation effects; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D013312:Stress, Physiological / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D005819:Genetic Markers