Selection of internal reference genes for SYBR green qRT-PCR studies of rhesus monkey (Macaca mulatta) tissues.
BACKGROUND
The rhesus monkey (Macaca mulatta) is a valuable and widely used model animal for biomedical research. However, quantitative analyses of rhesus gene expression profiles under diverse experimental conditions are limited by a shortage of suitable internal controls for the normalization of mRNA levels. In this study, we used a systematic approach for the selection of potential reference genes in the rhesus monkey and compared their suitability to that of the corresponding genes in humans.


RESULTS
Eight housekeeping genes (HKGs) (GAPDH, SDHA, ACTB, RPL13A, RPL32, UBA52, PGK1Y, and YWHAZ) from rhesus monkeys and humans were selected to test for normalization of expression levels in six different tissue types (brain, colon, kidney, liver, lung, and stomach). Their stability and suitability as reference genes were validated by geNorm, NormFinder and BestKeeper programs. Intriguingly, RPL13A and RPL32 were selected as ideal reference genes only in rhesus monkeys.


CONCLUSIONS
The results clearly indicated the necessity of using different reference genes for normalization of expression levels between rhesus monkeys and humans in various tissues.
Ahn
Kung
K
Division of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 609-735, Republic of Korea. ankung79@pusan.ac.kr
Huh
Jae-Won
JW
Park
Sang-Je
SJ
Kim
Dae-Soo
DS
Ha
Hong-Seok
HS
Kim
Yun-Ji
YJ
Lee
Ja-Rang
JR
Chang
Kyu-Tae
KT
Kim
Heui-Soo
HS
D000818:Animals; D005456:Fluorescent Dyes; D020869:Gene Expression Profiling; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005456:Fluorescent Dyes; D012333:RNA, Messenger
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