title: GLD2 poly(A) polymerase is required for long-term memory.
abstract: The formation of long-term memory is believed to require translational control of localized mRNAs. In mammals, dendritic mRNAs are maintained in a repressed state and are activated upon repetitive stimulation. Several regulatory proteins required for translational control in early development are thought to be required for memory formation, suggesting similar molecular mechanisms. Here, using Drosophila, we identify the enzyme responsible for poly(A) elongation in the brain and demonstrate that its activity is required specifically for long-term memory. These findings provide strong evidence that cytoplasmic polyadenylation is critical for memory formation, and that GLD2 is the enzyme responsible.
authors.affiliation: Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Jae Eun
authors.initials: JE
authors.lastname: Kwak
authors.forename: Eric
authors.initials: E
authors.lastname: Drier
authors.forename: Scott A
authors.initials: SA
authors.lastname: Barbee
authors.forename: Mani
authors.initials: M
authors.lastname: Ramaswami
authors.forename: Jerry C P
authors.initials: JC
authors.lastname: Yin
authors.forename: Marvin
authors.initials: M
authors.lastname: Wickens
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002478:Cells, Cultured; D003593:Cytoplasm / Q000201:enzymology; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology*; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000378:metabolism; D008568:Memory / Q000502:physiology*; D009046:Motor Neurons / Q000201:enzymology; D016501:Neurites / Q000201:enzymology; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D051860:Fragile X Messenger Ribonucleoprotein 1; D011062:Polynucleotide Adenylyltransferase; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C467328:FMR1 protein, Drosophila; C074333:pum protein, Drosophila
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