Design of an ultimate quencher free molecular beacon containing pyrrolocytidine-guanine base pair.
A novel quencher free molecular beacon was designed in which fluorophore-labelled pyrrolocytidine was placed away from the stem terminal. This new type of MB was used for the detection of a target DNA with an excellent efficiency.
Saito
Yoshio
Y
Department of Materials Chemistry and Engineering and NEWCAT Institute, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saito@chem.ce.nihon-u.ac.jp
Shinohara
Yuta
Y
Bag
Subhendu Sekhar
SS
Takeuchi
Yoshiki
Y
Matsumoto
Katsuhiko
K
Saito
Isao
I
D020029:Base Pairing; D003562:Cytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011758:Pyrroles; D003562:Cytidine; D006147:Guanine