In vivo murine model of continuous intramedullary infusion of particles--a preliminary study.
Continued production of wear debris affects both initial osseointegration and subsequent bone remodeling of total joint replacements (TJRs). However, continuous delivery of clinically relevant particles using a viable, cost effective, quantitative animal model to simulate the scenario in humans has been a challenge for orthopedic researchers. In this study, we successfully infused blue-dyed polystyrene particles, similar in size to wear debris in humans, to the intramedullary space of the mouse femur for 4 weeks using an osmotic pump. Approximately 40% of the original particle load (85 microL) was delivered into the intramedullary space, an estimate of 3 x 10(9) particles. The visible blue dye carried by the particles confirmed the delivery. This model demonstrated that continuous infusion of particles to the murine bone-implant interface is possible. In vivo biological processes associated using wear debris particles can be studied using this new animal model.
Ma
Ting
T
The Department of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.
Ortiz
Steven G
SG
Huang
Zhinong
Z
Ren
Peigen
P
Smith
R Lane
RL
Goodman
Stuart B
SB
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D016723:Bone Remodeling; D018786:Bone Substitutes / Q000737:chemistry*; D004396:Coloring Agents / Q000494:pharmacology; D016503:Drug Delivery Systems; D005269:Femur / Q000473:pathology*; D008297:Male; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D009995:Osmosis; D016348:Osseointegration; D010316:Particle Size; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D018786:Bone Substitutes; D004396:Coloring Agents; D014867:Water
J Bone Joint Surg Am. 2002 Feb;84(2):236-49
11861730
J Biomed Mater Res B Appl Biomater. 2004 Nov 15;71(2):360-6
15389497
J Orthop Res. 2002 Sep;20(5):1031-7
12382970
Acta Orthop Scand. 2002 Aug;73(4):416-24
12358115
J Bone Joint Surg Am. 1976 Jul;58(5):612-8
932060
J Orthop Res. 2007 Jul;25(7):841-8
17405158
J Bone Joint Surg Br. 1998 May;80(3):531-9
9619952
Spine (Phila Pa 1976). 2003 Oct 15;28(20):S125-38
14560184
J Biomed Mater Res B Appl Biomater. 2008 Feb;84(2):328-33
17595028
J Bone Joint Surg Am. 1990 Feb;72(2):252-8
1968067
Acta Orthop. 2005 Jun;76(3):412-20
16156472
Clin Orthop Relat Res. 1995 Feb;(311):46-53
7634590
Clin Orthop Relat Res. 1998 Jul;(352):46-52
9678032
Biomaterials. 2002 Jan;23(2):517-26
11761173
Biomaterials. 2006 Jul;27(21):3882-7
16540164
Clin Orthop Relat Res. 2004 Dec;(429):188-92
15577486
J Orthop Res. 2005 May;23(3):501-10
15885468
J Orthop Res. 2002 May;20(3):499-505
12038623
J Bone Joint Surg Am. 2003 Jun;85(6):1095-9
12784009
Biomaterials. 2005 Apr;26(11):1271-86
15475057
J Int Med Res. 2006 Nov-Dec;34(6):655-64
17294998
Biomaterials. 2004 Jan;25(2):269-75
14585714
Clin Orthop Relat Res. 1997 Nov;(344):33-43
9372756