Development of a novel capillary electrophoresis chemiluminescence system for amino acid analysis.
In the present study, a novel peroxyoxalate CE-CL system was developed to achieve high signal stability and sensitivity based on a design of a new interface including a new mixing mode and a new grounding electrode mode. Amino acids fluorescently tagged with dansyl chloride and naphthalene-2,3-dicarboxaldehyde(NDA) were used for the study. Experiment results show this new system is quite effective to separate and detect amine acid with high stability and resolute. The detection limits were 1.1 nmol/L for dansyl-leucine (Leu) and 2.0 nmol/L for dansyl-aspartic acid (Asp). The relative standard deviations of peak height and migration time were in the ranges of 2.3-3.8% and 1.2-1.5%, respectively.
Yin
Dongguang
D
College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, People's Republic of China. dongguang_yin@yahoo.com
Xie
Chunjuan
C
Zhang
Li
L
Liu
Binghu
B
Zhou
Xiaozhou
X
Wang
Peng
P
Wu
Minghong
M
D000596:Amino Acids / Q000032:analysis*; D001224:Aspartic Acid / Q000032:analysis; D003619:Dansyl Compounds / Q000737:chemistry*; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D007930:Leucine / Q000032:analysis; D008163:Luminescent Measurements / Q000379:methods*; D009281:Naphthalenes / Q000737:chemistry*; D010070:Oxalates / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
D000596:Amino Acids; D003619:Dansyl Compounds; D009281:Naphthalenes; D010070:Oxalates; D001224:Aspartic Acid; C044622:peroxyoxalate; C027717:2,3-naphthalenedicarboxaldehyde; D007930:Leucine; C029038:dansyl chloride