Direct and indirect roles for Nodal signaling in two axis conversions during asymmetric morphogenesis of the zebrafish heart.
The Nodal signaling pathway plays a conserved role in determining left-sided identity in vertebrates with this early left-right (L/R) patterning influencing the asymmetric development and placement of visceral organs. We have studied the role of Nodal signaling in asymmetric cardiac morphogenesis in zebrafish and describe two distinct rotations occurring within the heart. The first is driven by an asymmetric migration of myocardial cells during cardiac jogging, resulting in the conversion of the L/R axis to the dorsal-ventral (D/V) axis of the linear heart. This first rotation is directly influenced by the laterality of asymmetric gene expression. The second rotation occurs before cardiac looping and positions the original left cells exposed to Nodal signaling back to the left of the wild-type (WT) heart by 48 hours postfertilization (hpf). The direction of this second rotation is determined by the laterality of cardiac jogging and is not directly influenced by asymmetric gene expression. Finally, we have identified a role for Nodal signaling in biasing the location of the inner ventricular and outer atrial curvature formations. These results suggest that Nodal signaling directs asymmetric cardiac morphogenesis through establishing and subsequently reinforcing laterality information over the course of cardiac development.
Baker
Kari
K
Department of Molecular Biology, Princeton University, Washington Road MOF433, Princeton, NJ 08550, USA.
Holtzman
Nathalia G
NG
Burdine
Rebecca D
RD
D000818:Animals; D019521:Body Patterning; D002465:Cell Movement; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D055458:Left-Right Determination Factors; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D055457:Nodal Protein; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*
D055458:Left-Right Determination Factors; D055457:Nodal Protein; D016212:Transforming Growth Factor beta
Development. 2006 Aug;133(15):3015-25
16835440
Development. 2007 Mar;134(6):1023-34
17287255
Circ Res. 2008 Feb 1;102(2):e12-9
18202314
Semin Cell Dev Biol. 2002 Dec;13(6):507-13
12468254
Development. 2003 Jun;130(11):2303-16
12702646
Development. 1997 Nov;124(21):4373-82
9334285
Nat Rev Genet. 2001 Jan;2(1):39-48
11253067
Development. 2007 Apr;134(8):1605-15
17360770
Dev Biol. 2007 May 15;305(2):577-88
17395172
Dev Biol. 2006 Aug 15;296(2):437-49
16836994
J Am Soc Nephrol. 2008 Jun;19(6):1128-38
18385425
Science. 2003 Oct 24;302(5645):662-5
14576439
Development. 2007 Jun;134(12):2379-86
17537802
Development. 1999 Jun;126(14):3253-62
10375514
Dev Biol. 2008 Feb 15;314(2):261-75
18178183
Dev Biol. 1999 Oct 1;214(1):23-37
10491254
Dev Cell. 2008 Feb;14(2):287-97
18267096
Gene. 1999 Nov 15;240(1):239-44
10564832