Induction and removal of DNA damage in blood leukocytes of patients with type 2 diabetes mellitus undergoing hemodialysis.
Type 2 diabetes mellitus (T2DM) is associated with a high production of reactive oxygen species, which may cause oxidative DNA damage. High levels of genomic damage have been associated with renal failure and hemodialysis. However, no information is available in the literature concerning the levels of DNA damage in T2DM individuals who are dependent on hemodialysis. This study used the comet assay to assess the levels of DNA damage before, immediately after and 48 h after the hemodialysis session in 25 patients with T2DM and in a group of 20 healthy individuals, selected according to mean age, sex and smoking habit. Our results showed increased levels of DNA damage in hemodialysis-dependent T2DM individuals (12.36+/-8.04) when compared with healthy individuals (7.35+/-7.41) (p=0.014). Damage levels increased immediately after the hemodialysis session (19.76+/-12.40) (p=0.04), which suggests a possible action of pro-oxidative factors related to the therapy, with a genotoxic effect on cells. Results obtained 48 h after hemodialysis (6.44+/-5.99) evidenced damage removal (p=0.001), which may be suggestive of DNA repair.
Bagatini
Pamela Brambilla
PB
Health Science Institute, Feevale University, RS 239, 2755, CEP 93352-000, Novo Hamburgo, RS, Brazil.
Palazzo
Roberta Passos
RP
Rodrigues
Manoela Tressoldi
MT
Costa
Cynthia Hernandes
CH
Maluf
Sharbel Weidner
SW
D000368:Aged; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000628:therapy*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D006435:Renal Dialysis