Analysis of DNA denaturation.
This unit provides a detailed method for evaluating in situ DNA denaturation by flow cytometry. The principal technique is based upon the metachromatic properties of acridine orange. This technique has a number of advantages over traditional biochemical methods, but requires very precise methodology; the result is excellent clarity and differentiation between single-stranded and double-stranded DNA. This unit provides full details on the correct use of acridine orange together with an excellent discussion of the pitfalls and problems.
Darzynkiewicz
Z
Z
New York Medical College, Elmsford, New York, USA.
Juan
G
G
D000165:Acridine Orange / Q000032:analysis; D017209:Apoptosis; D002469:Cell Separation / Q000379:methods; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009691:Nucleic Acid Denaturation; D013696:Temperature; D013997:Time Factors
D004277:DNA, Single-Stranded; D004247:DNA; D000165:Acridine Orange