A maltooligosaccharide-forming amylase gene from Brachybacterium sp. strain LB25: cloning and expression in Escherichia coli.
Brachybacterium sp. strain LB25 produces a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents. The enzyme hydrolyzed starch to produce maltotriose primarily. The structural gene encoding the amylase from strain LB25 was cloned and sequenced. The amino acid sequence of the product showed significant similarity (45 to 49%) to amylases from the genus Streptomyces. The amylase gene was expressed in Escherichia coli, but the specific activity of the recombinant amylase was lower than that of the amylase purified from strain LB25.
Doukyu
Noriyuki
N
Bio-Nano Electronic Research Center, Toyo University, Saitama, Japan. doukyu@itakura.toyo.ac.jp
Yamagishi
Wataru
W
Kuwahara
Hirokazu
H
Ogino
Hiroyasu
H
D000192:Actinomycetales / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000681:Amylases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D005982:Glutathione Transferase / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism; D014312:Trisaccharides / Q000378:metabolism
D009844:Oligosaccharides; D011993:Recombinant Fusion Proteins; D014312:Trisaccharides; C021705:maltooligosaccharides; C008317:maltotriose; D005982:Glutathione Transferase; D000681:Amylases