Cystitis is associated with TRPV1b-downregulation in rat dorsal root ganglia.
The recently identified splice variant of the transient receptor vanilloid type 1 (TRPV1) molecule, TRPV1b, produces a negative-dominant effect on the responsiveness of the TRPV1 channel, which is increased by peripheral inflammatory processes. Here, we studied, using real-time polymerase chain reaction, whether cyclophosphamide injection-evoked cystitis is associated with altered TRPV1/TRPV1b expression in the L5-L6 dorsal root ganglia, which innervate the urinary bladder. We found that while TRPV1 mRNA expression was unchanged, the amount of TRPV1b transcript was significantly reduced in L5-L6 dorsal root ganglia during cystitis. These data indicate that peripheral inflammatory events induce changes in TRPV1b expression in primary sensory neurons, which may result in increased responsiveness of the TRPV1 channel.
Charrua
Ana
A
Institute of Histology and Embriology, University of Porto, Porto, Portugal.
Reguenga
Carlos
C
Paule
Cleoper C
CC
Nagy
Istvan
I
Cruz
Francisco
F
Avelino
António
A
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000633:toxicity; D003556:Cystitis / Q000139:chemically induced / Q000503:physiopathology*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005091:Exons / Q000235:genetics; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020869:Gene Expression Profiling; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000633:toxicity; D007249:Inflammation / Q000139:chemically induced / Q000503:physiopathology; D007274:Injections, Intraperitoneal; D008969:Molecular Sequence Data; D009475:Neurons, Afferent / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050916:TRPV Cation Channels / Q000235:genetics*; D001743:Urinary Bladder / Q000187:drug effects / Q000294:innervation / Q000378:metabolism; D017833:Visceral Afferents / Q000378:metabolism
D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D003520:Cyclophosphamide