Study of cysteinyl leukotriene-1 receptor in rat renal ischemia-reperfusion injury.
Renal ischemia-reperfusion (I/R) injury is a major cause of renal transplant dysfunction. Recent studies of I/R injury have focused on the function of neutrophils, the mechanisms of action of inflammatory cytokines, and oxygen free radicals, as well as other mediators. However, few reports address the cysteinyl leukotriene-1 receptor (CysLT1R), an important mediator of bronchial asthma in human beings. We examined the expression of CysLT1R in rat renal I/R injury. At laparotomy, the right kidney was harvested and the left renal artery and vein were clamped. The kidney was reperfused after 90 minutes of ischemia, and the rats were killed after 0, 3, 5, 12, or 24 hours. Expression of CysLT1R analyzed at immunohistochemistry was observed only in endothelial cells in nonischemic kidney. At 0 to 3 hours after reperfusion, CysLT1R expression on endothelial cells gradually became stronger, being most intense at 3 hours after reperfusion. Twelve hours after reperfusion, necrosis extended throughout the ischemic kidney; nearly all of the tubular epithelial cells were destroyed. At 3 to 12 hours after reperfusion, CysLT1R expression gradually became weaker on endothelial cells. At 24 hours after reperfusion, CysLT1R expression was almost at the level of that in nonischemic kidney. Expression of CysLT1R was noted in a rat model of renal I/R injury. Several hours after the maximal CysLT1R expression, we observed the maximum renal I/R injury. These results may suggest a relationship between the CysLT1R and renal I/R injury.
Matsuyama
M
M
Department of Transplantation and Clinical Immunology, Claude Bernard University of Lyon and Lyon Hospitals, Lyon, France.
Funao
K
K
Kawahito
Y
Y
Sano
H
H
Chargui
J
J
Touraine
J-L
JL
Takemoto
Y
Y
Nakatani
T
T
Yoshimura
R
R
D000818:Animals; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D007683:Kidney Tubular Necrosis, Acute / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D018077:Receptors, Leukotriene / Q000378:metabolism*; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000378:metabolism*
D018077:Receptors, Leukotriene; C083169:leukotriene D4 receptor