title: Use of partially porous column as second dimension in comprehensive two-dimensional system for analysis of polyphenolic antioxidants.
abstract: In the present work, a comprehensive LC system using a microbore HPLC column in the first dimension and a partially porous column in the second dimension was developed and applied to the separation of polyphenolic components in a red wine sample. The performance of the partially porous short column (3.0 cm) was compared to that of a monolithic column, of comparable dimensions. The results obtained demonstrated the possibility to use partially porous columns to obtain fast analyses, using high flow rates, under repetitive gradient conditions and with very brief reconditioning times. A conventional HPLC system was used since the backpressure generated by the shell-packed column, even at very high flow rates, was well within the operational limits. The use of an increased column temperature (60 degrees C) allowed a further pressure-drop decrease, with no stationary phase degradation, or loss in column performance.
authors.affiliation: Dipartimento di Scienze degli Alimenti e dell'Ambiente, Facoltà di Scienze, Università di Messina, Messina, Italy. pdugo@pharma.unime.it
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.forename: Francesco
authors.initials: F
authors.lastname: Cacciola
authors.forename: Miguel
authors.initials: M
authors.lastname: Herrero
authors.forename: Paola
authors.initials: P
authors.lastname: Donato
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D005419:Flavonoids / Q000032:analysis*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis*; D059808:Polyphenols; D014920:Wine
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols