title: Gene expression changes associated with xenobiotic metabolism pathways in mice exposed to acrylamide.
abstract: The discovery of acrylamide (AA) in a variety of fried foods has raised public health concerns. In this study, groups of male mice were administered 500 mg/L AA in drinking water for 3 weeks, and gene expression changes were evaluated in the livers of AA-treated mice within 24 hr of the last treatment. When a two-fold cutoff value and a P-value less than 0.05 were selected, 696 genes (233 up-regulated and 463 down-regulated) were identified as differentially expressed genes in AA-treated mice when compared with the controls. Gene ontology analysis revealed that the principle pathways affected by AA were xenobiotic metabolism by cytochrome P450 (CYPs) and glutathione metabolism, suggesting that drug and/or xenobiotic metabolism is most affected by exposure. The results provide more information about AA metabolism and further insight into the molecular mechanisms involved in AA-induced toxicity.
authors.affiliation: Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, Jefferson, Arkansas 72079, USA. nan.mei@fda.hhs.gov
authors.forename: Nan
authors.initials: N
authors.lastname: Mei
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Jo
authors.initials: J
authors.lastname: Tseng
authors.forename: Stacey L
authors.initials: SL
authors.lastname: Dial
authors.forename: Wayne
authors.initials: W
authors.lastname: Liao
authors.forename: Mugimane G
authors.initials: MG
authors.lastname: Manjanatha
mesh_terms: D020106:Acrylamide / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015262:Xenobiotics / Q000378:metabolism / Q000494:pharmacology
chemical_list: D015262:Xenobiotics; D020106:Acrylamide; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase
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