Titering antibodies.
Flow cytometry using fluorochrome-conjugated antibodies has emerged as a major approach to automated cellular identification. One of the most important issues in immunophenotyping is using the correct amount of antibody. This unit presents techniques for ascertaining the optimal titer for individual, dual, and multiple antibodies used for simultaneous phenotyping, stressing the importance of quality control in making batches of antibody for routine use.
Stewart
C C
CC
Roswell Park Cancer Institute, Buffalo, New York, USA.
Stewart
S J
SJ
D000911:Antibodies, Monoclonal / Q000737:chemistry; D000941:Antigens / Q000737:chemistry; D019921:Biotinylation; D000939:Epitopes / Q000737:chemistry; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007075:Immunoglobulin M / Q000737:chemistry; D016130:Immunophenotyping / Q000379:methods; D010641:Phenotype; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D007075:Immunoglobulin M