title: Drinking water: a possible source of Blastocystis spp. subtype 1 infection in schoolchildren of a rural community in central Thailand.
abstract: In January 2005, a survey of intestinal parasitic infections was performed in a primary school, central Thailand. Of 675 stool samples, Blastocystis was identified with a prevalence of 18.9%. Genetic characterization of Blastocystis showed subtype 1 (77.9%) and subtype 2 (22.1%). Study of the water supply in this school was performed to find the possible sources of Blastocystis. Blastocystis from one water sample was identified as subtype 1, which had a nucleotide sequence of small subunit (SSU) ribosomal RNA (rRNA) gene that was 100% identical to that of Blastocystis infected in schoolchildren. Our information supports the evidence of water-borne transmission in this population.
authors.affiliation: Department of Parasitology, Phramongkutklao College of Medicine, Ratchathewi, Bangkok, Thailand. s_leelayoova@scientist.com
authors.forename: Saovanee
authors.initials: S
authors.lastname: Leelayoova
authors.forename: Suradej
authors.initials: S
authors.lastname: Siripattanapipong
authors.forename: Umaporn
authors.initials: U
authors.lastname: Thathaisong
authors.forename: Tawee
authors.initials: T
authors.lastname: Naaglor
authors.forename: Paanjit
authors.initials: P
authors.lastname: Taamasri
authors.forename: Phunlerd
authors.initials: P
authors.lastname: Piyaraj
authors.forename: Mathirut
authors.initials: M
authors.lastname: Mungthin
mesh_terms: D000293:Adolescent; D000818:Animals; D016844:Blastocystis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016776:Blastocystis Infections / Q000453:epidemiology* / Q000469:parasitology; D002648:Child; D003430:Cross-Sectional Studies; D005243:Feces / Q000469:parasitology; D005260:Female; D005618:Fresh Water / Q000469:parasitology*; D005838:Genotype; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000453:epidemiology* / Q000469:parasitology; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D016053:RNA, Protozoan / Q000737:chemistry / Q000235:genetics; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D015183:Restriction Mapping; D012307:Risk Factors; D012424:Rural Population; D013785:Thailand / Q000453:epidemiology; D014881:Water Supply / Q000592:standards*
chemical_list: D016053:RNA, Protozoan; D012335:RNA, Ribosomal