title: Construction of Escherichia coli gene expression level perturbation collection.
abstract: We generated 61 strains of Escherichia coli in which the expression level of a specific single gene can be changed continuously over a physiologically significant range. In each strain, one auxotrophic gene was deleted from its original position and reinserted at a specific position on the chromosome under the control of the tetA promoter. Therefore, the level of expression of the target gene can be controlled easily by altering the concentrations of inducers, e.g., anhydrotetracycline and doxycycline, in the medium. Protein and mRNA levels and changes in proliferation rate were examined in some of the strains in our collection to determine the ability to control the level of target gene expression over a physiologically significant range. These strains will be useful for extracting omics data sets and for the construction of genome-scale mathematical models, because causality between perturbations in gene expression level and their consequences can be clearly determined.
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kashiwagi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sakurai
authors.forename: Saburo
authors.initials: S
authors.lastname: Tsuru
authors.forename: Bei-Wen
authors.initials: BW
authors.lastname: Ying
authors.forename: Kotaro
authors.initials: K
authors.lastname: Mori
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yomo
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic