title: Discordance between biochemical markers of liver activity and fibrosis (Actitest-Fibrotest) and liver biopsy in patients with chronic hepatitis C.
abstract: BACKGROUND
  The purpose of this clinical trial was to determine in routine practice and in comparison with liver biopsy the limitations of two blood tests, Actitest and Fibrotest, for the evaluation of hepatic activity and fibrosis in patients with chronic hepatitis C.
  
  
  METHODS
  Routine blood tests, Actitest and Fibrotest, and liver biopsy were performed in 96 patients with chronic hepatitis C attending routine outpatient clinics. Receiver operating characteristics (ROC) curves were used to assess the diagnostic value of the biochemical tests in comparison with the METAVIR classification.
  
  
  RESULTS
  The study population was predominantly male (63.5%) with a mean age of 48 years; 83.3% of the patients had genotype 1 hepatitis C virus infection. Treatment status was naive (62.5%), nonresponders (17.7%), relapsers (7.3%), or unknown (12.5%). The comparison of F0-F2 versus F3-F4 estimated the negative predictive value at 92% and the positive predictive value at 52% for a cut-off of 0.455. Discrepancies in activity score were more frequently due to a higher score of the biochemical test compared to biopsy (18 cases out of 19). Discrepancies for fibrosis were observed in 18 patients with a higher score for biochemical test in eight and a higher score for liver biopsy in 10 cases. A significant increase of gamma-glutamyl-transferase (GGT) (p=0.0001) and alanine aminotransferase (ALT) (p<0.0001) was observed in case of biochemical test overestimation of activity, and a significant increase of alpha2-macroglobulin (p=0.006) and GGT (p=0.018) in case of biochemical test overestimation of fibrosis.
  
  
  CONCLUSIONS
  This prospective study confirms the good diagnostic value of biochemical tests for necrotico-inflammatory activity and fibrosis as compared with the histological analysis of liver biopsy. Clinicians must interpret Actitest and Fibrotest results with caution in patients with a significant elevation of ALT, and/or GGT and/or alpha2-macroglobulin which could overestimate hepatic injury.
authors.affiliation: Centre hospitalier général, 93 rue de la Libération, Bourgoin-Jallieu, France. Fontanges@aol.com
authors.forename: T
authors.initials: T
authors.lastname: Fontanges
authors.forename: F
authors.initials: F
authors.lastname: Bailly
authors.forename: E
authors.initials: E
authors.lastname: Trepo
authors.forename: M
authors.initials: M
authors.lastname: Chevallier
authors.forename: M
authors.initials: M
authors.lastname: Maynard-Muet
authors.forename: B
authors.initials: B
authors.lastname: Nalet
authors.forename: S
authors.initials: S
authors.lastname: Beorchia
authors.forename: D
authors.initials: D
authors.lastname: Pillon
authors.forename: H
authors.initials: H
authors.lastname: Moindrot
authors.forename: B
authors.initials: B
authors.lastname: Froissart
authors.forename: M
authors.initials: M
authors.lastname: Slaoui
authors.forename: X
authors.initials: X
authors.lastname: Tinel
authors.forename: P
authors.initials: P
authors.lastname: Pradat
authors.forename: C
authors.initials: C
authors.lastname: Trepo
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D005260:Female; D019698:Hepatitis C, Chronic / Q000097:blood* / Q000473:pathology*; D006801:Humans; D008099:Liver / Q000473:pathology*; D008103:Liver Cirrhosis / Q000097:blood* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies
chemical_list: D015415:Biomarkers