title: Quantification of small cell numbers with a microchannel device.
abstract: The ability to precisely quantify rare populations of cells has become an essential first step of many cell-based assays in stem cell research. Since current devices for cell quantification require relatively high cell concentrations and/or absolute cell numbers, we have developed a microchannel-based device, allowing precise quantification of limiting cell numbers/concentrations. We anticipate this device will serve as an important tool to overcome a practical obstacle in stem cell research.
authors.affiliation: McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Nisha
authors.initials: N
authors.lastname: Badders
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yu
authors.forename: Caroline
authors.initials: C
authors.lastname: Alexander
authors.forename: David
authors.initials: D
authors.lastname: Beebe
mesh_terms: D002452:Cell Count; D002460:Cell Line; D004867:Equipment Design; D006801:Humans; D007668:Kidney / Q000166:cytology; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008858:Microscopy, Phase-Contrast; D008904:Miniaturization / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity
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