Fluorometric sensing of conformational switching of DNA; the use of fluorescence labeled C8-alkylamino substituted 2'-deoxyguanosine.
C8-alkylamino substituted 2'-deoxyguanosine was incorporated into a DNA sequence and then labeled with pyrene. The conformational change from B- to Z-form was monitored using CD and fluorescence spectroscopy for both labeled and unlabeled ODNs.
Saito
Yoshio
Y
Department of Materials Chemistry and Engineering, and NEWCAT Institute, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saito@chem.ce.nihon-u.ac.jp
Matsumoto
Katsuhiko
K
Bag
Subhendu Sekhar
SS
Misawa
Akihiro
A
Saito
Isao
I
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D043542:DNA, Z-Form / Q000737:chemistry*; D003849:Deoxyguanosine / Q000031:analogs & derivatives*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011721:Pyrenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D043542:DNA, Z-Form; D005456:Fluorescent Dyes; D011721:Pyrenes; D004247:DNA; D003849:Deoxyguanosine