title: Yak lactate dehydgogenase A4: purification, properties, and cDNA cloning.
abstract: Lactate dehydrogenase A4 (LDH-A4) was purified for yak skeletal muscle. Michaelis constant (Km) analysis showed that yak LDH-A4 for pyruvate was significantly higher than that of cattle. cDNA cloning of LDH-A revealed two amino acid substitutions between yak and cattle. We suggest that the higher Km of yak LDH-A4 might be a result of molecular adaptation to a hypoxic environment.
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, PR China. yucaizheng01@gmail.com
authors.forename: Yucai
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Si
authors.forename: Qinghua
authors.initials: Q
authors.lastname: He
authors.forename: Suyu
authors.initials: S
authors.lastname: Jin
authors.forename: Jian
authors.initials: J
authors.lastname: Hong
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D019344:Lactic Acid / Q000302:isolation & purification; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology; D019289:Pyruvic Acid / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D019344:Lactic Acid; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase