Derivation and characterization of nonhuman primate embryonic stem cells.
Embryonic stem (ES) cells are a powerful research tool enabling the generation of mice with custom genetics, the study of the earliest stages of mammalian differentiation in vitro and, with the isolation of human ES cells, the potential of cell-based therapies for a number of diseases including Parkinson's and Type 1 diabetes. ES cells isolated from nonhuman primates (nhpES cells) offer the opportunity to ethically test the developmental potential of primate ES cells in chimeric offspring. If these cells have similar potency to mouse ES cells, this may open a new era of primate models of human disease. Nonhuman primates are the perfect model system for the preclinical testing of ES cell-derived therapies. In this unit, we describe methods for the derivation and characterization of nonhuman primate ES cells. With these protocols, the investigator will be able to isolate nhpES cells and perform the necessary tests to confirm the pluripotent phenotype.
Navara
Christopher S
CS
University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
Redinger
Carrie
C
Mich-Basso
Jocelyn
J
Oliver
Stacie
S
Ben-Yehudah
Ahmi
A
Castro
Carlos
C
Simerly
Calvin
C
D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques; D002469:Cell Separation; D017931:DNA Primers / Q000235:genetics; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D015870:Gene Expression; D007150:Immunohistochemistry; D007621:Karyotyping; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010641:Phenotype; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D011323:Primates / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013724:Teratoma / Q000209:etiology; D014183:Transplantation, Heterologous
D017931:DNA Primers