title: Flow cytometric analysis of cytokine responses in stimulated whole blood: simultaneous quantitation of TNF-alpha-secreting cells and soluble cytokines.
abstract: This unit describes technical protocols aimed at the ex vivo or in vitro evaluation of the functional status of the immune system through the simultaneous identification and enumeration of cytokine-secreting cells and quantitation of the soluble cytokines produced by these cells.
authors.affiliation: Universidad de Salamanca, Salamanca, Spain.
authors.forename: Arancha
authors.initials: A
authors.lastname: Rodríguez-Caballero
authors.affiliation: Universidad de Salamanca, Salamanca, Spain.
authors.forename: Andrés
authors.initials: A
authors.lastname: García-Montero
authors.affiliation: Universidad de Salamanca, Salamanca, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Bueno
authors.affiliation: Universidad de Salamanca, Salamanca, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
mesh_terms: D035843:Biomedical Research / Q000379:methods; D001773:Blood Cells; D016207:Cytokines / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007107:Immune System; D007962:Leukocytes / Q000378:metabolism*; D008954:Models, Biological; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha