Manipulation of the peptide-binding specificity of an RNA in a rational manner by combinations of specificity-altering mutations.
In this study, the potential to manipulate the peptide-binding specificity of an RNA in a rational manner was investigated. First, variants of the Rev-response element (RRE) RNA with different specificities towards the natural binding partner, Rev, and two RRE-binding aptamers, the RSG-1.2 and K1 peptides, were identified. Next, hybrid RRE mutants with combinations of two sets of specificity-altering substitutions were tested for peptide-binding specificity. It was shown that, in most cases, the results of the combination of individual mutations were of an additive nature, therefore providing a way to manipulate the peptide-binding specificity of an RNA in a predictable manner.
Harada
Kazuo
K
Department of Life Science, Tokyo Gakugei University, Koganei-shi, Tokyo 184-8501, Japan. harada@u-gakugei.ac.jp
Sugaya
Maki
M
Nishimura
Futoshi
F
Katoh
Akira
A
D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism; D015752:Genes, env; D009154:Mutation; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism; D054321:rev Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000378:metabolism*
D052157:Aptamers, Nucleotide; D010455:Peptides; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus