title: [IgVH mutation status in patients with chronic lymphocytic leukemia].
abstract: OBJECTIVE
  To evaluate the frequency and mutation status of IgVH gene expression in patients with chronic lymphocytic leukemia (CLL) in China.
  
  
  METHODS
  IgVH mutation was detected by multiplex PCR and directly sequencing in 29 CLL patients. IgH somatic hypermutation and mutation site were analysed by IMGT/V-QUEST.
  
  
  RESULTS
  Of 29 CLL patients, 21 had IgVH mutation (72%). The most frequently expressed VH gene family was found to be VH3 (55%) followed by VH4 (38%), VH2 (3.5%) and VH7 (3.5%), with no expression of VH1, VH5 and VH6.
  
  
  CONCLUSIONS
  The expression frequency of IgVH gene families in Chinese CLL patients is significantly different from that in Western CLL patients, suggesting the involvement of ethnic and/or environmental factors in CLL development, which might partly explain the different incidence of CLL between China and Western countries.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Zheng
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Wu
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Qiu
authors.forename: Si-Xuan
authors.initials: SX
authors.lastname: Qian
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002908:Chronic Disease; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation
chemical_list: D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region