Iso-thioacetamido nucleic acids (isoTANA): synthesis and DNA binding studies of lyxo/ribo oligonucleotides.
In the previous study, we developed a method to synthesize 5-atom thioacetamido linked TANA dimer blocks. Incorporation of the dimers in chimeric TANA-phosphodiester linked oligomers and the binding studies towards DNA/RNA preferences were reported. The selectivity towards RNA was found to be significantly high compared to DNA. The lyxo isomer of 3'-amino substitution in thymine was found to have higher 3'-endo conformation in monomer units. In this paper we present studies on synthesis of 3'-lyxo-3'-amino isomer of TANA, its tst and cst dimer units, and incorporation in oligomers. Preliminary results on DNA/RNA binding of these new backbone modified oligomers will be presented.
Gokhale
Sachin
S
Division of Organic Chemistry, National Chemical Laboratory, Pune 411008, India.
Kumar
Vaijayanti A
VA
D004247:DNA / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D013936:Thymidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
D009841:Oligonucleotides; D012313:RNA; D004247:DNA; D013936:Thymidine