Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay.
This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C(T)) values of >35. IS481 PCRs with C(T) values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.
Guthrie
J L
JL
Ministry of Health and Long-Term Care, 81 Resources Rd., Toronto, Ontario M9P 3T1, Canada. jennifer.guthrie@ontario.ca
Seah
C
C
Brown
S
S
Tang
P
P
Jamieson
F
F
Drews
S J
SJ
D001426:Bacterial Proteins / Q000235:genetics*; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D004251:DNA Transposable Elements; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D012680:Sensitivity and Specificity; D014917:Whooping Cough / Q000175:diagnosis*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements
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