Establishment and characterization of a bona fide Barrett esophagus-associated adenocarcinoma cell line.
Esophageal adenocarcinoma currently has one of the most rapidly increasing tumor incidences in the United States, with the vast majority of cases occurring on the backdrop of metaplastic epithelium (Barrett esophagus). The availability of appropriate cell line models is essential for maintaining the pace of esophageal cancer research and for pre-clinical validation of new therapeutic modalities. The identity of several of the widely utilized esophageal adenocarcinoma cell lines (BIC-1, SEG-1 and TE-7) have recently been called into question. Here we describe the establishment and characterization of a bona fide esophageal cancer cell line, JH-EsoAd1, from a patient with Barrett-associated adenocarcinoma. The rapid dissemination of this cancer cell line to the esophageal cancer research community should help ameliorate the current scarcity of preclinical models in this disease.
Alvarez
Hector
H
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Koorstra
Jan-Bart M
JB
Hong
Seung-Mo
SM
Boonstra
Jurjen J
JJ
Dinjens
Winand N M
WN
Foratiere
Arlene A
AA
Wu
Tsung-Teh
TT
Montgomery
Elizabeth
E
Eshleman
James R
JR
Maitra
Anirban
A
D000230:Adenocarcinoma / Q000150:complications* / Q000473:pathology; D000368:Aged; D000818:Animals; D001471:Barrett Esophagus / Q000150:complications* / Q000473:pathology; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000150:complications / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008858:Microscopy, Phase-Contrast / Q000379:methods; D009368:Neoplasm Transplantation; D014746:Vimentin / Q000096:biosynthesis
D014746:Vimentin
Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10578-82
1359541
Cancer Cell. 2005 May;7(5):469-83
15894267
Cancer Res. 2007 Sep 1;67(17):7996-8001
17804709
Br J Cancer. 1997;75(2):258-63
9010035
Science. 2007 Feb 16;315(5814):928-31
17303729
Br J Cancer. 2007 May 21;96(10):1579-86
17426708
J Surg Res. 2000 Feb;88(2):120-4
10644476
CA Cancer J Clin. 2008 Mar-Apr;58(2):71-96
18287387
J Pathol. 1994 Jan;172(1):5-12
7931827
Cancer Res. 1984 Dec;44(12 Pt 1):5813-21
6498841
Science. 1992 May 1;256(5057):668-70
1350108
J Thorac Cardiovasc Surg. 2007 Mar;133(3):763-9
17320581
Nat Rev Mol Cell Biol. 2000 Dec;1(3):233-6
11252900
Cancer Cell. 2004 Jul;6(1):11-6
15261138