title: Shading correction: compensation for illumination and sensor inhomogeneities.
abstract: The interaction between objects in real space, the illumination, and the camera frequently leads to a situation in which a microscope image exhibits significant shading across the field of view. In general this shading effect is undesirable and requires elimination, especially for quantitative microscopy. Starting with a simple mathematical model, this unit develops procedures to correct images for the shading thus introduced in the image-formation process. Two cases are distinguished: the a priori, which assumes the availability of calibration images in addition to the images of interest; and the a posteriori, which assumes that only the recorded images of interest are available.
authors.affiliation: Delft University of Technology, Delft, The Netherlands.
authors.forename: I T
authors.initials: IT
authors.lastname: Young
mesh_terms: D000465:Algorithms; D002138:Calibration; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D015233:Models, Statistical; D008962:Models, Theoretical