Correlation between organic acid exudation and metal uptake by ectomycorrhizal fungi grown on pond ash in vitro.
Experiments were conducted to investigate the effect of coal ash on organic acid exudation and subsequent metal uptake by ectomycorrhizal fungi. Four isolates of ectomycorrhizal fungi namely, Pisolithus tinctorius (EM-1293 and EM-1299), Scleroderma verucosum (EM-1283) and Scleroderma cepa (EM-1233) were grown on pond ash moistened with Modified Melin-Norkans medium in vitro. Exudation of formic acid, malic acid and succinic acid by these fungi were detected by HPLC. Mycelial accumulation of Al, As, Cd, Cr, Ni and Pb by these fungi was assayed by atomic absorption spectrophotometer. Relationship between organic acid exudation and metal uptake was determined using classical multivariate linear regression model. Correlation between organic acid exudation and metal uptake could be substantiated when several metals are considered collectively. The finding supports the widespread role of low molecular weight organic acid as a function of tolerance, when exposed to metals in vitro.
Ray
Prasun
P
Centre for Mycorrhizal Research, The Energy and Resources Institute, India Habitat Centre, D S Block, Lodhi Road, New Delhi, 110003, India. prasun.ray@gmail.com
Adholeya
Alok
A
D000143:Acids / Q000737:chemistry*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D005561:Formates / Q000737:chemistry; D008293:Malates / Q000737:chemistry; D008670:Metals; D019216:Metals, Heavy / Q000737:chemistry*; D008970:Molecular Weight; D038821:Mycorrhizae / Q000378:metabolism*; D009930:Organic Chemicals / Q000737:chemistry*; D052638:Particulate Matter; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry; D019802:Succinic Acid / Q000737:chemistry; D014874:Water Pollutants, Chemical
D000143:Acids; D005561:Formates; D008293:Malates; D008670:Metals; D019216:Metals, Heavy; D009930:Organic Chemicals; D052638:Particulate Matter; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; C030544:formic acid; C030298:malic acid; D019802:Succinic Acid