Expression profile of microRNAs in c-Myc induced mouse mammary tumors.
c-Myc is a transcription factor overexpression of which induces mammary cancer in transgenic mice. To explore whether certain microRNAs (mirRNA) mediate c-Myc induced mammary carcinogenesis, we studied mirRNA expression profile in mammary tumors developed from MMTV-c-myc transgenic mice, and found 50 and 59 mirRNAs showing increased and decreased expression, respectively, compared with lactating mammary glands of wild type mice. Twenty-four of these mirRNAs could be grouped into eight clusters because they had the same chromosomal localizations and might be processed from the same primary RNA transcripts. The increased expression of mir-20a, mir-20b, and mir-9 as well as decreased expression of mir-222 were verified by RT-PCR, real-time RT-PCR, and cDNA sequencing. Moreover, we fortuitously identified a novel non-coding RNA, the level of which was decreased in proliferating mammary glands of MMTV-c-myc mice was further decreased to undetectable level in the mammary tumors. Sequencing of this novel RNA revealed that it was transcribed from a region of mouse chromosome 19 that harbored the metastasis associated lung adenocarcinoma transcript-1 (Malat-1), a non-protein-coding gene. These results suggest that certain mirRNAs and the chromosome 19 derived non-coding RNAs may mediate c-myc induced mammary carcinogenesis.
Sun
Yuan
Y
Hormel Institute, University of Minnesota, Austin, MN, 55912, USA.
Wu
Jack
J
Wu
Si-hung
SH
Thakur
Archana
A
Bollig
Aliccia
A
Huang
Yong
Y
Liao
D Joshua
DJ
D000818:Animals; D002472:Cell Transformation, Viral / Q000235:genetics; D002874:Chromosome Mapping; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D007774:Lactation / Q000235:genetics; D008321:Mammary Glands, Animal / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000235:genetics*; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics*
D035683:MicroRNAs; D012334:RNA, Neoplasm
Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9136-41
16754881
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2999-3004
14973191
Genes Chromosomes Cancer. 1995 Aug;13(4):229-38
7547630
Oncogene. 2007 Aug 30;26(40):5877-88
17384685
Mol Cells. 2005 Oct 31;20(2):157-66
16267388
Nature. 2005 Jun 9;435(7043):839-43
15944709
Development. 2008 Apr;135(7):1201-14
18287206
J Hum Genet. 2003;48(6):331-335
12721791
Proc Natl Acad Sci U S A. 1987 May;84(9):2970-4
3495006
Histol Histopathol. 2007 Jun;22(6):661-76
17357096
J Pathol. 2008 Jul;215(3):214-21
18446835
Pediatr Dev Pathol. 2005 Nov-Dec;8(6):615-20
16328670
Cancer Res. 2005 Aug 15;65(16):7065-70
16103053
J Pathol. 2006 Sep;210(1):49-58
16823893
Mol Cell Biol. 2006 Nov;26(21):8191-201
16940181
Int J Cancer. 2008 Feb 1;122(3):699-704
17943719
Cancer Res. 2007 Jun 1;67(11):5061-3
17545579
Genomics. 1999 Sep 1;60(2):238-40
10486216
Cancer Res. 1998 Feb 15;58(4):743-52
9485030
Cancer Sci. 2008 May;99(5):986-94
18380791
Nature. 2005 Jun 9;435(7043):828-33
15944707
Mol Cancer. 2008 Jan 24;7:11
18218118
Int J Exp Pathol. 2006 Apr;87(2):151-61
16623759
Nat Cell Biol. 2005 Dec;7(12):1261-6
16284623
Oncogene. 1994 May;9(5):1447-53
8152806
Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2257-61
16461460
Crit Rev Oncog. 2007 Nov;13(2):93-158
18197790
Cancer Res. 2008 Jul 15;68(14):5540-5
18632605
Cancer Res. 2004 May 1;64(9):3087-95
15126345
Cancer Res. 2006 Aug 1;66(15):7390-4
16885332
Oncogene. 2007 Sep 6;26(41):6099-105
17384677
Genes Chromosomes Cancer. 1997 Nov;20(3):234-42
9365830
Nat Genet. 2008 Mar;40(3):281-3
18264098
Int J Cancer. 2002 May 20;99(3):361-9
11992404
Endocrinology. 2002 Jun;143(6):2029-40
12021166
Cell Cycle. 2007 Aug 15;6(16):2010-8
17700064
J Korean Med Sci. 1998 Jun;13(3):311-6
9681812
Biochem Biophys Res Commun. 2006 Apr 28;343(1):85-9
16529715
Nat Genet. 2000 Feb;24(2):197-200
10655070
Cell. 2005 Jul 15;122(1):9-12
16009127
Science. 2005 Sep 2;309(5740):1519-24
16141061
Biochem Biophys Res Commun. 2005 Aug 26;334(2):319-23
16036130
Cytogenet Cell Genet. 1995;71(3):280-4
7587394
Oncol Rep. 2006 Aug;16(2):417-21
16820925
Nature. 2005 Jun 9;435(7043):834-8
15944708
Cancer Res. 2005 Nov 1;65(21):9628-32
16266980
Cell Oncol. 2004;26(5-6):307-17
15623941
J Transl Med. 2004 Sep 21;2(1):32
15383145
Angew Chem Int Ed Engl. 2006 Aug 4;45(31):5048-50
16858714
Proc Natl Acad Sci U S A. 2005 May 31;102(22):7940-5
15905330
Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17834-9
17093053
Biotechnol J. 2007 Jan;2(1):33-5
17124719
Genomics. 1999 Mar 15;56(3):340-3
10087202
Hum Mol Genet. 2008 Mar 1;17(5):642-55
18006640
Mod Pathol. 2005 May;18(5):638-44
15540119
Cancer Invest. 2003;21(4):641-58
14533452
Genomics. 2002 Jan;79(1):41-50
11827456
Mol Biotechnol. 2006 Mar;32(3):197-204
16632886
Nat Genet. 2008 Jan;40(1):43-50
18066065
Genomics. 2003 Dec;82(6):628-36
14611804
Glycobiology. 2001 Mar;11(3):19R-23R
11320054
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D140-4
16381832
Endocr Relat Cancer. 2000 Sep;7(3):143-64
11021963
Proc Natl Acad Sci U S A. 2007 May 8;104(19):8017-22
17470785
Int J Cancer. 2006 Sep 1;119(5):1052-60
16570289
Am J Med Genet. 1991 Dec 15;41(4):557-65
1723247