STEAP mRNA detection in serum of patients with solid tumours.
STEAP was identified by the strategy of suppression subtractive hybridizations in Los Angeles prostate cancer xenografts. It is expressed in prostate and other cancers, and not in most normal tissue; it can be used as a marker to evaluate biological samples from individuals suspected of having a disease associated with STEAP dysregulation, such as cancers, and may provide prognostic information useful in defining appropriate therapeutic options. The aim of this study was to test the STEAP mRNA detection in the serum of patients with different malignant tumours by using Real-Time reverse transcription PCR. The results were compared with biological samples obtained by age-matched non-malignant donors. Our data demonstrated that STEAP mRNA is detectable in serum of patients with different solid tumours whereas it is not amplifiable in non-malignant donors. This marker revealed with the molecular method of quantitative PCR in serum, may be useful to discriminate normal and cancer patients.
Valenti
M T
MT
Department of Biomedical and Surgical Sciences, Clinic of Internal Medicine D, University of Verona, Piazzale Scuro 10, 37134 Verona, Italy.
Dalle Carbonare
L
L
Donatelli
L
L
Bertoldo
F
F
Giovanazzi
B
B
Caliari
F
F
Lo Cascio
V
V
D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000097:blood* / Q000235:genetics; D014408:Biomarkers, Tumor; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000175:diagnosis*; D010088:Oxidoreductases / Q000097:blood* / Q000235:genetics; D012333:RNA, Messenger / Q000097:blood; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D010088:Oxidoreductases; C432763:STEAP1 protein, human