Chromosomal regions underlying noncoagulation of milk in Finnish Ayrshire cows.
About 10% of Finnish Ayrshire cows produce noncoagulating milk, i.e., milk that does not form a curd in a standard 30-min testing time and is thus a poor raw material for cheese dairies. This phenomenon is associated with peak and midlactation, but some cows produce noncoagulating milk persistently. A genomewide scan under a selective DNA pooling method was carried out to locate genomic regions associated with the noncoagulation of milk. On the basis of the hypothesis of the same historical mutation, we pooled the data across sires. Before testing pools for homogeneity, allele intensities were corrected for PCR artifacts, i.e., shadow bands and differential amplification. Results indicating association were verified using daughter design and selective genotyping within families. Data consisted of 18 sire families with 477 genotyped daughters in total, i.e., 12% of each tail of the milk coagulation ability. Data were analyzed using interval mapping under maximum-likelihood and nonparametric methods. BMS1126 on chromosome 2 and BMS1355 on chromosome 18 were associated with noncoagulation of milk across families on an experimentwise 0.1% significance level. By scanning gene databases, we found two potential candidate genes: LOC538897, a nonspecific serine/threonine kinase on chromosome 2, and SIAT4B, a sialyltransferase catalyzing the last step of glycosylation of kappa-casein on chromosome 18. Further studies to determine the role of the candidates in the noncoagulation of milk are clearly needed.
Tyrisevä
Anna-Maria
AM
Department of Animal Science, University of Helsinki, FI-00014 Helsinki, Finland. anna-maria.tyriseva@helsinki.fi
Elo
Kari
K
Kuusipuro
Arja
A
Vilva
Veijo
V
Jänönen
Isto
I
Karjalainen
Heidi
H
Ikonen
Tiina
T
Ojala
Matti
M
D000818:Animals; D001947:Breeding; D002364:Caseins / Q000235:genetics; D002417:Cattle / Q000235:genetics*; D002611:Cheese; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics*; D005260:Female; D005387:Finland; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000737:chemistry* / Q000378:metabolism
D002364:Caseins
Heredity (Edinb). 1999 Nov;83 (Pt 5):613-24
10620035
J Dairy Sci. 2006 Sep;89(9):3296-305
16899662
J Dairy Res. 2007 May;74(2):167-73
17291390
J Dairy Sci. 1999 Jan;82(1):205-14
10022022
J Dairy Sci. 2004 Feb;87(2):458-67
14762089
J Dairy Sci. 2001 May;84(5):1027-33
11384028
Genetics. 2003 Mar;163(3):1169-75
12663553
Genetics. 1995 Mar;139(3):1421-8
7768449
Proteomics. 2006 May;6(10):3087-95
16619295
Cell Mol Life Sci. 2006 Feb;63(3):378-89
16429323
Genetics. 1994 Dec;138(4):1365-73
7896115
Theor Appl Genet. 1990 Nov;80(5):631-4
24221069
J Dairy Sci. 2008 Jan;91(1):371-6
18096961
Theor Appl Genet. 1992 Nov;85(2-3):353-9
24197326
Vet Rec. 1977 Oct 15;101(16):325-6
563124
Biotechniques. 1991 Apr;10(4):506-13
1867860
J Dairy Res. 1997 May;64(2):231-8
9161915
Ann Hum Genet. 1995 Jan;59(1):97-105
7762987
J Dairy Sci. 2004 Nov;87(11):3958-66
15483181
Genome Res. 2000 Jan;10(1):105-15
10645955
J Dairy Res. 2003 Feb;70(1):91-8
12617397
Genetics. 1998 Jul;149(3):1547-55
9649541
Genetics. 1998 Jul;149(3):1557-67
9649542
Nucleic Acids Res. 1988 Feb 11;16(3):1215
3344216
J Dairy Res. 2006 May;73(2):171-7
16476179
Genetics. 1995 Feb;139(2):907-20
7713441
J Dairy Sci. 2004 Feb;87(2):443-9
14762087
J Dairy Sci. 1985 Oct;68(10):2527-33
3934240
Genetics. 2007 Nov;177(3):1963-6
17720902
J Dairy Sci. 1993 Jul;76(7):2025-32
8345129
J Dairy Sci. 1991 Sep;74(9):2879-87
1779047