Extraction of green labeled pectins and pectic oligosaccharides from plant byproducts.
Green labeled pectins were extracted by an environmentally friendly way using proteases and cellulases being able to act on proteins and cellulose present in cell walls. Pectins were isolated from different plant byproducts, i.e., chicory roots, citrus peel, cauliflower florets and leaves, endive, and sugar beet pulps. Enzymatic extraction was performed at 50 degrees C for 4 h, in order to fulfill the conditions required for microbiological safety of extracted products. High methoxy (HM) pectins of high molar mass were extracted with three different enzyme mixtures. These pectins were subsequently demethylated with two pectin methyl esterases (PMEs), either the fungal PME from Aspergillus aculeatus or the orange PME. It was further demonstrated that high molar mass low methoxy (LM) pectins could also be extracted directly from cell walls by adding the fungal PME to the mixture of protease and cellulase. Moreover, health benefit pectic oligosaccharides, the so-called modified hairy regions, were obtained after enzymatic treatment of the residue recovered after pectin extraction. The enzymatic method demonstrates that it is possible to convert vegetable byproducts into high-added value compounds, such as pectins and pectic oligosaccharides, and thus considerably reduce the amount of these residues generated by food industries.
Zykwinska
Agata
A
INRA, UR1268 Biopolymeres Interactions Assemblages, F-44300 Nantes, France.
Boiffard
Marie-Hélène
MH
Kontkanen
Hanna
H
Buchert
Johanna
J
Thibault
Jean-François
JF
Bonnin
Estelle
E
D001230:Aspergillus / Q000201:enzymology; D027461:Beta vulgaris / Q000737:chemistry; D001937:Brassica / Q000737:chemistry; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D044602:Cellulases / Q000378:metabolism; D018651:Cichorium intybus / Q000737:chemistry; D002957:Citrus / Q000737:chemistry / Q000201:enzymology; D009844:Oligosaccharides / Q000302:isolation & purification*; D010368:Pectins / Q000302:isolation & purification*; D010447:Peptide Hydrolases / Q000378:metabolism; D010944:Plants / Q000737:chemistry*
D009844:Oligosaccharides; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D044602:Cellulases; D010447:Peptide Hydrolases