Selective recovery of target cells employing a bi-functional aptamer.
Aptamers are single-stranded nucleic acids with high and specific affinity to a target molecule. Recently, target cell-binding aptamers are studied as molecular tools for the detection and the isolation of desired cells. Here, I developed a method for the selective recovery of target cells employing a bi-functional aptamer. As a model experiment, I constructed the bi-functional aptamer by fusing a resin-binding aptamer and a target cell-binding aptamer. The bi-functional aptamer enabled the selective recovery of the target cells simply by mixing of the cells with the bi-functional aptamer and the resin. This simple method would be applied for a variety of cell types and resins.
Ohuchi
Shoij P
SP
Department of Basic Medical Sciences, IMSUT, The University of Tokyo, Minato-ku, Tokyo 108-0071, Japan. sohuchi@ims.u-tokyo.ac
D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D016466:CHO Cells; D002469:Cell Separation / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D003911:Dextrans; D005434:Flow Cytometry; D007202:Indicators and Reagents
D052157:Aptamers, Nucleotide; D003911:Dextrans; D007202:Indicators and Reagents; C025614:sephadex