Coordinate expression of the human pregnancy-specific glycoprotein gene family during induced and replicative senescence.
Pregnancy-specific glycoproteins (PSGs) comprise a family of highly similar polypeptides encoded by 11 transcriptionally active genes that compactly cluster on band 19q13.2. All members of the PSG family were found to be markedly up-regulated by addition of 5-bromodeoxyuridine in HeLa cells. Similarly, all of the members were markedly up-regulated during replicative senescence in normal human fibroblasts. Promoter analysis of the PSG1, 4, and 11 genes in HeLa cells did not reveal a cis-regulatory element responsive to 5-bromodeoxyuridine in their 5'-flanking sequences. These results suggest that the PSG genes are regulated at a level of higher order chromatin structure besides by a signal of pregnancy.
Endoh
Morio
M
International Graduate School of Arts and Sciences, Yokohama City University, Seto 22-2, Kanazawa-ku, Yokohama, Kanagawa, 236-0027, Japan.
Kobayashi
Yusuke
Y
Yamakami
Yoshimi
Y
Yonekura
Ryuzo
R
Fujii
Michihiko
M
Ayusawa
Dai
D
D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation; D016922:Cellular Senescence; D004249:DNA Damage; D005786:Gene Expression Regulation; D005838:Genotype; D006367:HeLa Cells; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D010641:Phenotype; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D011268:Pregnancy-Specific beta 1-Glycoproteins; D012333:RNA, Messenger; D001973:Bromodeoxyuridine