title: Antibacterial effects of green tea polyphenols on clinical isolates of methicillin-resistant Staphylococcus aureus.
abstract: The antibacterial effects of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were evaluated. Characterization of the minimal inhibitory concentration (MIC) of oxacillin for 30 S. aureus strains isolated from patients treated with oxacillin identified 13 strains with an oxacillin MIC >or= 4 microg/mL as methicillin-resistant Staphylococcus aureus (MRSA) (range: 8 to 512 microg/mL), while 17 strains were methicillin-susceptible Staphylococcus aureus (MSSA) (range: 0.25-0.5 microg/mL). The MICs of TPP ranged from 50 to 180 microg/mL for both the MSSA and the MRSA strains. The MICs of oxacillin for each of the 13 MRSA strains were reduced between 8- and 128-fold when these strains were coincubated with sub-MIC (<or= 0.5x MIC) levels of TPP, demonstrating that the combination of TPP plus oxacillin was synergistic for all of the clinical MRSA isolates. Two-dimensional polyacrylamide gel electrophoresis identified 14 extracellular proteins of MRSA-13 down-regulated and 3 proteins up-regulated by exposure to TPP. These studies demonstrate that TPP can differentially stimulate the expression of various proteins in these bacteria and synergize the bactericidal activity of oxacillin for MRSA.
authors.affiliation: Department of Biotechnology, Soonchunhyang University, P.O. Box 97, Asan, Chung-Nam 336-600, Republic of Korea.
authors.forename: Yun-Seok
authors.initials: YS
authors.lastname: Cho
authors.forename: Neal L
authors.initials: NL
authors.lastname: Schiller
authors.forename: Kye-Heon
authors.initials: KH
authors.lastname: Oh
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000032:analysis; D028241:Camellia sinensis / Q000737:chemistry*; D015169:Colony Count, Microbial; D015180:Electrophoresis, Gel, Two-Dimensional; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D010068:Oxacillin / Q000494:pharmacology / Q000627:therapeutic use; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols; D020543:Proteome / Q000032:analysis; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D020543:Proteome; D010068:Oxacillin
references.citation: Biochim Biophys Acta. 1993 Apr 8;1147(1):132-6
references.pmid: 8466924
references.citation: Appl Environ Microbiol. 1995 Aug;61(8):2863-72
references.pmid: 16535093
references.citation: Proteomics. 2006 Feb;6(4):1301-18
references.pmid: 16470664
references.citation: Int J Antimicrob Agents. 2004 May;23(5):462-7
references.pmid: 15120724
references.citation: Electrophoresis. 1999 Dec;20(18):3551-67
references.pmid: 10612281
references.citation: Antimicrob Agents Chemother. 2002 Jul;46(7):2266-8
references.pmid: 12069986
references.citation: Curr Microbiol. 2002 Dec;45(6):415-22
references.pmid: 12402082
references.citation: Antimicrob Agents Chemother. 2004 Feb;48(2):546-55
references.pmid: 14742208
references.citation: Antimicrob Agents Chemother. 2001 Jun;45(6):1737-42
references.pmid: 11353619
references.citation: Curr Microbiol. 2004 Nov;49(5):346-52
references.pmid: 15486709
references.citation: Antimicrob Agents Chemother. 2005 Jul;49(7):2914-20
references.pmid: 15980368
references.citation: Proteomics. 2002 Jun;2(6):740-6
references.pmid: 12112857
references.citation: Antimicrob Agents Chemother. 2005 Oct;49(10):4339-43
references.pmid: 16189116
references.citation: Int J Antimicrob Agents. 2004 Oct;24(4):374-80
references.pmid: 15380264
references.citation: J Antimicrob Chemother. 2002 Dec;50(6):1051-4
references.pmid: 12461032
references.citation: J Antimicrob Chemother. 2001 Sep;48(3):361-4
references.pmid: 11533000
references.citation: Appl Microbiol Biotechnol. 2004 Aug;65(3):323-9
references.pmid: 15309341
references.citation: Curr Opin Infect Dis. 2006 Apr;19(2):161-8
references.pmid: 16514341
references.citation: Antimicrob Agents Chemother. 2002 Feb;46(2):558-60
references.pmid: 11796378
references.citation: Electrophoresis. 2001 May;22(9):1705-11
references.pmid: 11425226
references.citation: J Bacteriol. 1997 Dec;179(23):7595-9
references.pmid: 9393730
references.citation: Curr Microbiol. 2007 Dec;55(6):501-6
references.pmid: 17874165
references.citation: Antimicrob Agents Chemother. 2005 Feb;49(2):549-55
references.pmid: 15673731