Incorporation of 3'-S-phosphorothiolates into RNA: potential applications in RNAi.
Using solid-phase synthesis, oligoribonucleotides containing multiple 3'-S-phosphorothiolate modifications have been successfully synthesized, purified and characterized, utilizing a 2'-deoxyuridine phosphorothioamidite monomer.
Gaynor
James
J
Department of Chemistry, University of Liverpool, Crown Street, Liverpool, L69 7ZD, UK. jwgaynor@liv.ac.uk
Fisher
Julie
J
Campbell
Barry
B
Cosstick
Richard
R
D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis* / Q000737:chemistry; D013873:Thionucleotides / Q000138:chemical synthesis / Q000737:chemistry; D013891:Thiouridine / Q000737:chemistry*; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors
C465503:3'-S-phosphorothiolate; D009843:Oligoribonucleotides; D010710:Phosphates; D034741:RNA, Small Interfering; D013873:Thionucleotides; D051996:Transcription Factor RelA; D013891:Thiouridine