title: Reconstitution of iron oxidase from sulfur-grown Acidithiobacillus ferrooxidans.
abstract: The iron oxidation system from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells was reconstituted in vitro. Purified rusticyanin, cytochrome c, and aa(3)-type cytochrome oxidase were essential for reconstitution. The iron-oxidizing activity of the reconstituted system was 3.3-fold higher than that of the cell extract from which these components were purified.
authors.affiliation: Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan. tahery1@yahoo.com
authors.forename: Taher M
authors.initials: TM
authors.lastname: Taha
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Kanao
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Takeuchi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sugio
mesh_terms: D042763:Acidithiobacillus / Q000201:enzymology*; D001400:Azurin / Q000302:isolation & purification / Q000378:metabolism; D045304:Cytochromes c / Q000302:isolation & purification / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000302:isolation & purification / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D010088:Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: C012543:rusticyanin; D001400:Azurin; D045304:Cytochromes c; D007501:Iron; D010088:Oxidoreductases; D003576:Electron Transport Complex IV
references.citation: J Biochem. 1992 Dec;112(6):816-21
references.pmid: 1338330
references.citation: Biosci Biotechnol Biochem. 2004 Dec;68(12):2519-28
references.pmid: 15618623
references.citation: Biochim Biophys Acta. 1989 Sep 28;976(2-3):129-34
references.pmid: 2551385
references.citation: Biosci Biotechnol Biochem. 1999 Sep;63(9):1541-7
references.pmid: 10540740
references.citation: FEMS Microbiol Lett. 2002 Apr 9;209(2):189-95
references.pmid: 12007804
references.citation: Eur J Biochem. 2001 Mar;268(6):1653-8
references.pmid: 11248684
references.citation: J Bacteriol. 1992 Jun;174(12):4189-92
references.pmid: 1597434
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713
references.citation: Microbiology (Reading). 2004 Jul;150(Pt 7):2113-2123
references.pmid: 15256554
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: Biochem J. 1978 Aug 15;174(2):497-502
references.pmid: 708402
references.citation: Biochim Biophys Acta. 2004 Jun 7;1656(2-3):114-26
references.pmid: 15178473
references.citation: Biosci Biotechnol Biochem. 2007 Nov;71(11):2663-9
references.pmid: 17986795