Influence of the carbon source on the growth and lignocellulolytic enzyme production by Morchella esculenta strains.
Growth and lignocellulolytic enzymes production by two Morchella esculenta strains (BAFC 1728 and BEL 124) growing in solid state fermentation using different lignocellulosic materials along 58 days was characterized. Both strains were able to grow on the three substrates: wheat bran, wheat bran plus corn starch, and rolled oat. The growth was characterized by measuring chitin content, reducing sugars, pH, dry weight loss, and extractable proteins, such parameters varied substantially with substrate and strain used. The maximum rate of growth in both strains was observed between 5 and 28 days. Regarding enzyme production, as a general trend strain BAFC 1728 produced the highest titres. The most evident difference was observed in laccase production by this strain on wheat bran, which exceeded that observed in strain BEL 124 by tenfold (7.45 U g(-1)).
Papinutti
Leandro
L
Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Buenos Aires, Argentina. leandru@bg.fcen.uba.ar
Lechner
Bernardo
B
D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development*; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D004043:Dietary Fiber / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis; D007218:Industrial Microbiology; D042845:Laccase / Q000096:biosynthesis*; D013379:Substrate Specificity
D003470:Culture Media; D004043:Dietary Fiber; D005656:Fungal Proteins; D002244:Carbon; D042845:Laccase
Anal Biochem. 1976 May 7;72:248-54
942051
Arch Biochem Biophys. 1985 Nov 1;242(2):329-41
4062285
Science. 1983 Aug 12;221(4611):661-3
17787736
Appl Microbiol Biotechnol. 2006 Jan;69(5):573-9
16021487
Appl Environ Microbiol. 1996 Apr;62(4):1151-8
8919775
Enzyme Microb Technol. 2001 Jan 2;28(1):81-88
11118601
Mycol Res. 2006 May;110(Pt 5):612-23
16769512
Appl Environ Microbiol. 1989 Dec;55(12):3095-100
16348070