STAT92E is a positive regulator of Drosophila inhibitor of apoptosis 1 (DIAP/1) and protects against radiation-induced apoptosis.
The proapoptotic factors Reaper, Hid, Grim, and Sickle regulate apoptosis in Drosophila by inhibiting the antiapoptotic factor DIAP1 (Drosophila inhibitor of apoptosis 1). Heat, UV light, x-rays, and developmental signals can all increase the proapoptotic factors, but the control of transcription of the diap1 gene is unclear. We show that in imaginal discs the single Drosophila STAT protein (STAT92E) when activated can directly increase DIAP1 through binding to STAT DNA-binding sites in the diap1 promoter. The STAT92E contribution to DIAP1 production is required for cell survival after x-irradiation but not under unstressed conditions. Because DIAP1 prevents apoptosis after a variety of stresses, STAT92E may have a role in regulating stress responses in general.
Betz
Aurel
A
Laboratory of Molecular Cell Biology, The Rockefeller University, 1230 York Ave, New York, NY 10065, USA.
Ryoo
Hyung Don
HD
Steller
Hermann
H
Darnell
James E
JE
D000818:Animals; D030801:Animals, Genetically Modified; D017209:Apoptosis / Q000528:radiation effects*; D001483:Base Sequence; D001665:Binding Sites; D029721:Drosophila Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D005123:Eye / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000235:genetics; D050791:STAT Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
C111891:DIAP1 protein, Drosophila; D029721:Drosophila Proteins; D051034:Inhibitor of Apoptosis Proteins; D050791:STAT Transcription Factors; C105675:Stat92E protein, Drosophila
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