title: Techniques for mammalian cell tissue culture.
abstract: This unit opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
authors.affiliation: Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Phelan
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media / Q000592:standards*; D005615:Freezing; D006434:Hemocytes / Q000166:cytology / Q000502:physiology; D008322:Mammals; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media