Stability and mismatch discrimination of DNA duplexes containing 2,6-diaminopurine and 2-thiothymidine locked nucleic acid bases.
Hybridization thermodynamics measured by differential scanning calorimetry (DSC) and UV spectroscopy (UVM) are reported for 8- and 14-mer oligonucleotides containing two new LNA bases: 2,6 diaminopurine (D) and 2-thiothymidine (2sT). Oligonucleotides containing D or 2sT bases are shown to have enhanced stability and improved discrimination for several of the possible mismatched base pairs.
Hughesman
Curtis B
CB
Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada.
Turner
Robin F B
RF
Haynes
Charles
C
D015075:2-Aminopurine / Q000031:analogs & derivatives* / Q000737:chemistry; D020137:Base Pair Mismatch; D002152:Calorimetry, Differential Scanning; D004247:DNA / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry
D015345:Oligonucleotide Probes; D009841:Oligonucleotides; C477371:locked nucleic acid; C060625:2-thiothymidine; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D004247:DNA; D013936:Thymidine