The effect of weight reduction on plasma concentrations of ghrelin and insulin-like growth factor 1 in obese women.
BACKGROUND
The aim of the present study was to examine how weight loss treatment modulates plasma concentrations of ghrelin and insulin-like growth factor 1 (IGF-1) in obese women and to determine whether there is any association with possible changes in plasma concentrations of these hormones after weight loss.


METHODS
The study group consisted of 22 obese women without additional disease (age 40.6 +/- 12.9 years; BMI 37.2 +/- 4.6 kg/m(2)). All subjects participated in a 3-month weight reduction program. The measurements were performed at baseline and after weight loss. Plasma concentration of ghrelin and IGF-1 were measured by enzyme - linked immunosorbent assay (ELISA) kit. Serum concentrations of insulin were measured by radioimmunoassay (RIA). Body composition was determined by bioelectrical impedance analysis using a Bodystat analyser.


RESULTS
The mean weight loss was 9.3 +/- 4.1 kg (9.7 +/- 4.3%). Following weight loss, plasma ghrelin and IGF-1 concentrations increased significantly (63.5 +/- 13.0 vs. 72.8 +/- 15.1 pg/ml; p < 0.01; 126.9 +/- 67.0 vs. 170.5 +/- 83.3 ng/ml p < 0.01, respectively) and serum insulin concentrations decreased significantly (17.5 +/- 8.5 vs. 14.8 +/- 10.4 mIU/ml p< 0.05). We observed a significant positive correlation between the increase of ghrelin and decrease of body fat percentage after weight loss (r = 0.44, p = 0.03). There are no correlations between change of ghrelin and IGF-1concentrations and between changes of insulin and IGF 1 concentrations.


CONCLUSIONS
Plasma concentrations of ghrelin and IGF-1 increased after weight loss. However, it seems there is no association between serum concentrations of ghrelin and IGF-1 in obese women.
Olszanecka-Glinianowicz
Magdalena
M
Department of Pathophysiology, Medical University of Silesia, Katowice, Poland. bzachorska@sum.edu.pl
Zahorska-Markiewicz
Barbara
B
Kocełak
Piotr
P
Janowska
Joanna
J
Semik-Grabarczyk
Elzbieta
E
D000328:Adult; D005260:Female; D054439:Ghrelin / Q000097:blood*; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000097:blood*; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000628:therapy*; D015431:Weight Loss / Q000502:physiology*
D054439:Ghrelin; C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1