Synthesis, biophysical and repair studies of O6-2'-deoxyguanosine adducts by Escherichia coli OGT.
Oligonucleotides containing modified 2'-deoxyguanosines bearing a seven carbon linker at the O(6)- atom with either a terminal hydroxyl or 2'- deoxyguanosine group have been synthesized as potential intermediates formed during repair of interstrand cross-linked DNA. Repair of these substrates with Escherichia coli OGT was investigated with an assay involving cleavage of the unmodified duplex with the restriction endonuclease PvuII followed by analysis of the products by denaturing polyacrylamide gel electrophoresis. Duplexes containing these modifications were repaired by OGT suggesting that direct repair may play a role, in combination with other repair pathways, in reversing interstrand crosslink DNA damage.
Schoonhoven
Nadia M
NM
Department of Chemistry & Biochemistry, Concordia University, 7141 Sherbooke St. West, Montreal, Canada.
Murphy
Sebastian P
SP
O'Flaherty
Derek K
DK
Noronha
Anne M
AM
Kornblatt
M Judith
MJ
Wilds
Christopher J
CJ
D018736:DNA Adducts / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D003849:Deoxyguanosine / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism*; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000378:metabolism*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism
D018736:DNA Adducts; D029968:Escherichia coli Proteins; D009841:Oligonucleotides; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase; C067511:Ogt protein, E coli; D003849:Deoxyguanosine