[N-ras and fms gene mutation in idiopathic thrombocytopenic purpura and myelodysplasia].
OBJECTIVE
To explore the pathogenesis of idiopathic thrombocytopenic purpura (ITP) and improve the differential diagnosis from myelodysplastic syndromes (MDS).


METHODS
Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was performed to detect the point mutation of codon 12,13 in N-ras gene and codon 301, 969 in fms gene in adult and aged ITP and MDS patients.


RESULTS
In 25 ITP patients, N-ras mutation and fms mutation were detected in one each (4%). Mutations were found in 3 of 8 MDS patients: two (25%) with N-ras mutation and one (12.5%) with fms mutation.


CONCLUSIONS
Patients with N-ras or fms gene mutation diagnosed as MDS rather than ITP.
Zhao
Hong-Yu
HY
Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China.
Hou
Ming
M
Li
Xiao-Fang
XF
Ma
Dao-Xin
DX
Liu
Qi-Ji
QJ
Wang
Pin
P
D000328:Adult; D000368:Aged; D005260:Female; D016236:Genes, fms / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics*