title: YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules.
abstract: BACKGROUND
  YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs.
  
  
  RESULTS
  We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicated that YB-1 most probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Finally, we demonstrated that tubulin interferes with RNA:YB-1 complexes.
  
  
  CONCLUSIONS
  These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation.
authors.affiliation: Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, INSERM/UEVE U829 Evry, 91025 France. pcurmi@univ-evry.fr
authors.forename: Konstantin G
authors.initials: KG
authors.lastname: Chernov
authors.forename: Alain
authors.initials: A
authors.lastname: Mechulam
authors.forename: Nadezhda V
authors.initials: NV
authors.lastname: Popova
authors.forename: David
authors.initials: D
authors.lastname: Pastre
authors.forename: Elena S
authors.initials: ES
authors.lastname: Nadezhdina
authors.forename: Olga V
authors.initials: OV
authors.lastname: Skabkina
authors.forename: Nina A
authors.initials: NA
authors.lastname: Shanina
authors.forename: Victor D
authors.initials: VD
authors.lastname: Vasiliev
authors.forename: Anne
authors.initials: A
authors.lastname: Tarrade
authors.forename: Judith
authors.initials: J
authors.lastname: Melki
authors.forename: Vandana
authors.initials: V
authors.lastname: Joshi
authors.forename: Sonia
authors.initials: S
authors.lastname: Baconnais
authors.forename: Flavio
authors.initials: F
authors.lastname: Toma
authors.forename: Lev P
authors.initials: LP
authors.lastname: Ovchinnikov
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Curmi
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D018625:Microscopy, Atomic Force; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure*; D009687:Nuclear Proteins / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011817:Rabbits; D012261:Ribonucleoproteins / Q000378:metabolism; D014020:Tissue Extracts; D014404:Tubulin / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D051840:Y-Box-Binding Protein 1
chemical_list: D004268:DNA-Binding Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D014020:Tissue Extracts; D014404:Tubulin; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; C046279:tubulin S
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