Detection of Escherichia coli and Salmonella typhimurium using interdigitated microelectrode capacitive immunosensors: the importance of transducer geometry.
This paper presents an immunosensing system to detect Escherichia coli and Salmonella based on electrochemical impedance spectroscopy at interdigitated electrode structures. Our results show the importance of good electrode design in the final detection limit. Four different structures have been fabricated and functionalized. Biotinylated polyclonal antibodies have been immobilized on neutravidin-coated chips, and BSA has been used to avoid nonspecific adsorption. The immunosensor may be said to be capacitive since it is that part of the impedance used to monitor the presence of bacteria in phosphate buffer solution samples. Detection limits around 10(4)-10(5) cells mL(-1) have been reached using chips featuring interdigitated structures of less than 10 microm wide and 1.5 mm long. In both cases, the detection limits of the corresponding ELISA assays, using the same antibodies, was 1 order of magnitude higher (10(5)-10(6) cells mL(-1)). The analysis time, including sensor preparation was less than 5 h.
Laczka
Olivier
O
Instituto de Microelectrónica de Barcelona, IMB-CNM, (CSIC), Esfera UAB, Campus Universitat Autónoma de Barcelona, 08193 Barcelona, Spain.
Baldrich
Eva
E
Muñoz
Francesc Xavier
FX
del Campo
Fco Javier
FJ
D000918:Antibody Specificity; D001431:Bacteriological Techniques / Q000379:methods*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification*; D008839:Microelectrodes; D012486:Salmonella typhimurium / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity