title: Effect of locked nucleic acid (LNA) modification on hybridization kinetics of DNA duplex.
abstract: The effect of locked nucleic acid (LNA) modification on hybridization kinetics of DNA duplex formation has been studied at varying salt concentration (Na(+) and Mg(2+)) using surface-plasmon resonance. The study suggested that the increased stability of LNA containing duplexes mainly originates from the slower dissociation rates constants of the duplexes. An increase in salt concentration increased the binding affinity of the individual duplexes by raising their association rate constants. Monitoring the change in binding affinity with respect to salt concentration revealed that of LNA-associated enhancement in helical stability mainly results from the changes in the non-electrostatic interactions upon duplex formation.
authors.affiliation: Institute of Genomics and Integrative Biology, CSIR, Mall Road, Delhi 110007, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Arora
authors.forename: Harleen
authors.initials: H
authors.lastname: Kaur
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
authors.forename: Souvik
authors.initials: S
authors.lastname: Maiti
mesh_terms: D004247:DNA / Q000737:chemistry*; D007700:Kinetics; D015636:Magnesium Chloride / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: D009841:Oligonucleotides; C477371:locked nucleic acid; D015636:Magnesium Chloride; D012965:Sodium Chloride; D004247:DNA