title: Conducting polymer-based impedimetric aptamer biosensor for in situ detection.
abstract: We have successfully developed the first prototype of an electrochemical protein biosensor using polypyrrole as the substrate and doped aptamer as the probe. The sensitivity for a specific target is 10 ng/ml. Two targets, platelet-derived growth factor and immunoglobulin E, have been tested. In both cases the sensor exhibited high specificity and the signal was found to increase as the target concentration increased.
authors.affiliation: Department of Bioengineering, University of Pittsburgh, 5063 Bioscience Tower 3, 3501 Fifth Ave, Pittsburgh, PA 15260, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Liao
authors.forename: Brad A
authors.initials: BA
authors.lastname: Randall
authors.forename: Nicolas A
authors.initials: NA
authors.lastname: Alba
authors.forename: Xinyan Tracy
authors.initials: XT
authors.lastname: Cui
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis* / Q000737:chemistry; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry; D007073:Immunoglobulin E / Q000032:analysis*; D017403:In Situ Hybridization / Q000379:methods*; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D010982:Platelet-Derived Growth Factor; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D007073:Immunoglobulin E