Acute exercise increases expression of extracellular superoxide dismutase in skeletal muscle and the aorta.
Exercise dramatically increases oxygen consumption and causes oxidative stress. Superoxide dismutase (SOD) is important in the first-line defence mechanisms against oxidative stress. To investigate the effect of acute exercise on the expression of SOD, we examined the expression of mRNA for three SOD isozymes, in mice run on a treadmill to exhaustion. Six hours after exercise, the expression of extracellular SOD (EC-SOD) mRNA increased significantly in skeletal muscle and persisted for 24 h, whereas no change was observed for cytoplasmic and mitochondrial SOD mRNA. Moreover, acute exercise also induced EC-SOD mRNA in the aorta. These results suggest that a single bout of exercise is enough to augment the expression EC-SOD mRNA in skeletal muscle and the aorta, and may partly explain the beneficial effect of exercise.
Hitomi
Yoshiaki
Y
Department of Molecular Predictive Medicine and Sport Science, School of Medicine, Kyorin University, Mitaka, Tokyo, Japan.
Watanabe
Sumiko
S
Kizaki
Takako
T
Sakurai
Takuya
T
Takemasa
Tohru
T
Haga
Shukoh
S
Ookawara
Tomomi
T
Suzuki
Keiichiro
K
Ohno
Hideki
H
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000201:enzymology*; D005082:Physical Exertion; D012333:RNA, Messenger / Q000378:metabolism; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; C499090:Sod3 protein, mouse; D013482:Superoxide Dismutase