Titanium acts on osteoblast translational process.
Titanium is a highly biocompatible material and very osteogenic in vivo. However, how titanium regulates osteoblast activity to promote bone formation is incompletely characterized. We, therefore, attempted to get more information by using microRNA (miRNA) microarray techniques to investigate translation regulation in osteoblasts grown on titanium disks. The miRNA oligonucleotide microarray provides a novel method to carry out genome-wide miRNA profiling in human samples. By using miRNA microarrays containing 329 probes designed from the human miRNA sequence, several miRNA were identified in osteoblast-like cell line (MG 63) grown on titanium disks. There were 13 upregulated miRNAs (ie, mir-23a, mir-222, mir-523, mir-22, mir-23b, mir-143, mir-377, mir-24, mir-422b, mir-26a, mir-29a, mir-17-5p, mir-182) and 2 down-regulated miRNAs (ie, mir-187, mir-339). The data reported are, to our knowledge, the first study on translation regulation in osteoblasts exposed to titanium. The data can be relevant to understand better the molecular mechanism of osteoblast activation and as a model for comparing other materials with similar clinical effects.
Palmieri
Annalisa
A
Institute of Histology, University of Bologna and Center of Molecular Genetics, Cassa di Risparmio di Bologna Foundation, Bologna, Italy.
Pezzetti
Furio
F
Avantaggiato
Anna
A
Lo Muzio
Lorenzo
L
Scarano
Antonio
A
Rubini
Corrado
C
Guerzoni
Laura
L
Arlotti
Marzia
M
Ventorre
Dario
D
Carinci
Francesco
F
D001672:Biocompatible Materials / Q000494:pharmacology*; D002460:Cell Line; D003764:Dental Materials / Q000494:pharmacology*; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000187:drug effects*; D014176:Protein Biosynthesis / Q000187:drug effects*; D015347:RNA Probes; D014025:Titanium / Q000494:pharmacology*; D015854:Up-Regulation / Q000235:genetics
D001672:Biocompatible Materials; D003764:Dental Materials; D015347:RNA Probes; D014025:Titanium