title: Thermodynamic investigations on shape selective separation behaviors of poly(4-vinylpyridine)-grafted silica for polycyclic aromatic hydrocarbons in both normal-phase and reversed-phase high-performance liquid chromatography.
abstract: With the successful implementation of poly(4-vinylpyridine)-grafted silica prepared by grafting-from approach (GF-VP(n)) as a stationary phase for the separation of polycyclic aromatic hydrocarbons (PAHs) in normal-phase HPLC, this paper describes the chromatographic retention behaviors of PAHs with GF-VP(n) in reversed-phase HPLC. Significantly higher retention factor along with enhanced shape selectivity were observed with GF-VP(n). Thermodynamic study on the retention behaviors of PAHs with GF-VP(n) in normal-phase and reversed-phase HPLC revealed that retention of PAHs was exothermic in both phases. Furthermore, higher entropic contribution was observed in reversed-phase HPLC compared to normal-phase HPLC.
authors.affiliation: Department of Applied Chemistry and Biochemistry, Kumamoto University, Kurokami, Kumamoto, Japan.
authors.forename: Usha Ghimire
authors.initials: UG
authors.lastname: Gautam
authors.forename: Mani Prasad
authors.initials: MP
authors.lastname: Gautam
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sawada
authors.forename: Makoto
authors.initials: M
authors.lastname: Takafuji
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Ihara
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000302:isolation & purification*; D011145:Polyvinyls / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D011145:Polyvinyls; C019535:poly(4-vinylpyridine); D012822:Silicon Dioxide