[The distribution and phenotype of bone marrow-derived cells in mice's eyes after induction of choroidal neovascularization by laser photocoagulation].
OBJECTIVE
To investigate the phenotype feature of bone marrow-derived cells in mice's eyes after induction of choroidal neovascularization by laser photocoagulation.


METHODS
It was a experimental study. Green fluorescent protein (GFP) chimeric mice were developed by transplanting bone marrow cells from GFP +/+ transgenic mice to adult C57BL/6J mice. The chimeric mice underwent laser rupture of Bruch membrane to induce CNV. Fluorescein fundus angiography and histopathological study were used to confirm the stable formation of CNV. Then the eyes were enucleated and processed for immunofluorescence to detect the distribution and phenotype of GFP + cells.


RESULTS
The development of CNV has stabled by the 14th day after lasering. GFP-labelled cells appeared in CNV lesions (including choroid beneath CNV lesion), neurosensory retina over CNV, corneoscleral limbus, ciliary body, optic disc and sclera, retina and choroid distant from CNV. GFP + cells, which were immunoreactive for alphaSMA or CD31, appeared in lesions only. However, F4/80 + green cells can be also detected in neurosensory retina over CNV, corneoscleral limbus and ciliary body.


CONCLUSIONS
BMC which differentiated into vascular cells presented in CNV lesions only. Some BMC appearing in other positions might be macrophages or dendritic cells. There may be other functions apart from contributing to choroidal neovascularization for BMC in the eyes.
Hou
Hui-Yuan
HY
Department of Ophthalmology, Eye Institute of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Wang
Yu-Sheng
YS
Xu
Jian-Feng
JF
Zhang
Peng
P
Su
Xiao-Na
XN
Wang
Yao-Chun
YC
Liu
Jin-Ping
JP
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002829:Choroid / Q000473:pathology*; D020256:Choroidal Neovascularization / Q000209:etiology / Q000473:pathology*; D003713:Dendritic Cells / Q000166:cytology; D005260:Female; D017075:Laser Coagulation / Q000009:adverse effects; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic