title: 2'-Methylseleno modified oligoribonucleotides synthesized for X-ray crystallography.
abstract: Site-specifically modified 2'-methylseleno RNA represents a valuable derivative for phasing of X-ray crystallographic data. Several successful applications in three-dimensional structure determination of nucleic acids, such as the Diels-Alder ribozyme,(1) have relied on these modifications.(2) Currently, preparation of 2'-methylseleno modified oligoribonucleotides is restricted to the 2'-O-[(triisopropylsilyl)oxy]methyl (TOM)(3,4,5) and 2'-O-tert.-butyldimethylsilyl (TBDMS) RNA synthesis methods. Recently, we have introduced synthetic routes to 2'-methylseleno phosphoramidite building blocks of all four standard nucleosides, adenosine, uridine, guanosine, cytidine, that are tailored for the 2'-O-bis(acetoxyethoxy)methyl (ACE) RNA solid-phase synthesis method.(6).
authors.affiliation: Institute of Organic Chemistry, Center for Molecular Biosciences Innsbruck, Leopold Franzens University Innsbruck, Innrain 52a, Innsbruck, Austria.
authors.forename: Holger
authors.initials: H
authors.lastname: Moroder
authors.forename: Barbara
authors.initials: B
authors.lastname: Puffer
authors.forename: Ronald
authors.initials: R
authors.lastname: Micura
mesh_terms: D018360:Crystallography, X-Ray; D006151:Guanosine / Q000031:analogs & derivatives / Q000737:chemistry; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016566:Organoselenium Compounds / Q000737:chemistry*
chemical_list: C522827:2'-methylseleno guanosine; D009843:Oligoribonucleotides; D016566:Organoselenium Compounds; D006151:Guanosine