Complex N-glycans or core 1-derived O-glycans are not required for the expression of stage-specific antigens SSEA-1, SSEA-3, SSEA-4, or Le(Y) in the preimplantation mouse embryo.
The glycan epitopes termed stage-specific embryonic antigens (SSEA) occur on glycoproteins and glycolipids in mammals. However, it is not known whether these epitopes are attached to N- or O-glycans on glycoproteins and/or on glycolipids in the developing mouse embryo. In this paper the expression of the antigens SSEA-1, SSEA-3, SSEA-4 and Le(Y) was examined on ovulated eggs, early embryos and blastocysts lacking either complex and hybrid N-glycans or core-1 derived O-glycans. In all cases, antigen expression determined by fluorescence microscopy of bound monoclonal antibodies to embryos at the stage of development of maximal expression was similar in mutant and control embryos. Thus, none of these developmental antigens are expressed solely on either complex N- or core 1-derived O-glycans attached to glycoproteins in the preimplantation mouse embryo. Furthermore, neither of these classes of glycan is essential for the expression of SSEA-1, SSEA-3, SSEA-4 or Le(Y) on mouse embryos.
Williams
Suzannah A
SA
Department of Cell Biology, Albert Einstein College of Medicine, New York, NY 10461, USA.
Stanley
Pamela
P
D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D002236:Carbohydrate Conformation; D005260:Female; D007983:Lewis Blood Group Antigens / Q000378:metabolism*; D016256:Lewis X Antigen / Q000378:metabolism; D051379:Mice; D009154:Mutation / Q000235:genetics; D017351:N-Acetylglucosaminyltransferases; D010063:Ovum / Q000166:cytology / Q000201:enzymology; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism*
D015295:Antigens, Tumor-Associated, Carbohydrate; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; C094162:Lewis Y antigen; D011134:Polysaccharides; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3; C066584:stage-specific embryonic antigen-4; D000217:Acyltransferases; C459903:MGAT1 protein, mouse; D017351:N-Acetylglucosaminyltransferases
Biochem Biophys Res Commun. 1981 Jun;100(4):1578-86
6117281
FASEB J. 2008 Jul;22(7):2273-84
18276833
Glycoconj J. 2004;21(1-2):41-5
15467397
Genesis. 2000 Feb;26(2):110-2
10686600
Bioessays. 1990 Apr;12(4):173-9
1970725
Adv Exp Med Biol. 1982;152:167-77
6127917
Nature. 1985 Mar 7-13;314(6006):53-7
2579340
J Exp Med. 1984 Nov 1;160(5):1591-6
6491606
J Embryol Exp Morphol. 1985 Dec;90:335-61
3009679
Eur Urol. 1993;23(1):30-6; discussion 36-7
7682952
Cell. 1982 Oct;30(3):697-705
6183004
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15631-6
14673092
Differentiation. 1988 Jul;38(2):124-33
3209003
Histochem J. 1993 Jun;25(6):452-63
7689550
Dev Biol. 1987 Jun;121(2):568-75
2884155
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565-9
281705
Cell Differ. 1985 May;16(3):169-73
2860973
Mol Cell Biol. 2004 May;24(10):4221-8
15121843
J Exp Zool. 1987 Dec;244(3):395-408
3443830
Mol Cell Biol. 2004 Nov;24(22):9920-9
15509794
Mol Hum Reprod. 1998 Mar;4(3):295-300
9570276
J Biol Chem. 1983 Jul 25;258(14):8934-42
6863318
Curr Top Dev Biol. 1979;13 Pt 1:139-65
396115
J Biol Chem. 1983 Oct 10;258(19):11793-7
6194156
Genesis. 2005 Dec;43(4):175-80
16283624
Dev Biol. 1984 Aug;104(2):449-60
6745495
J Cell Sci. 2007 Apr 15;120(Pt 8):1341-9
17374637
Development. 2004 Sep;131(18):4435-45
15306566
Glycobiology. 1997 Oct;7(7):913-9
9363433
J Cell Biochem. 1988 Jan;36(1):1-14
3277983
Biol Reprod. 1995 Apr;52(4):903-12
7780013
Biochem J. 1983 Dec 1;215(3):491-503
6197967
Dev Biol. 1984 May;103(1):117-28
6370756
EMBO J. 1983;2(12):2355-61
6141938
Nature. 1981 Jul 9;292(5819):156-8
6165896
Histochem J. 1993 Sep;25(9):628-41
7693623