title: Annexin A1 regulates intestinal mucosal injury, inflammation, and repair.
abstract: During mucosal inflammation, a complex array of proinflammatory and protective mechanisms regulates inflammation and severity of injury. Secretion of anti-inflammatory mediators is a mechanism that is critical in controlling inflammatory responses and promoting epithelial restitution and barrier recovery. AnxA1 is a potent anti-inflammatory protein that has been implicated to play a critical immune regulatory role in models of inflammation. Although AnxA1 has been shown to be secreted in intestinal mucosal tissues during inflammation, its potential role in modulating the injury/inflammatory response is not understood. In this study, we demonstrate that AnxA1-deficient animals exhibit increased susceptibility to dextran sulfate sodium (DSS)-induced colitis with greater clinical morbidity and histopathologic mucosal injury. Furthermore, impaired recovery following withdrawal of DSS administration was observed in AnxA1 (-/-) animals compared with wild-type (WT) control mice that was independent of inflammatory cell infiltration. Since AnxA1 exerts its anti-inflammatory properties through stimulation of ALX/FPRL-1, we explored the role of this receptor-ligand interaction in regulating DSS-induced colitis. Interestingly, treatment with an ALX/FPRL-1 agonist, 15-epi-lipoxin A4 reversed the enhanced sensitivity of AnxA1 (-/-) mice to DSS colitis. In contrast, 15-epi-lipoxin A4 did not significantly improve the severity of disease in WT animals. Additionally, differential expression of ALX/FPLR-1 in control and DSS-treated WT and AnxA1-deficient animals suggested a potential role for AnxA1 in regulating ALX/FPRL-1 expression under pathophysiological conditions. Together, these results support a role of endogenous AnxA1 in the protective and reparative properties of the intestinal mucosal epithelium.
authors.affiliation: Epithelial Pathobiology Research Unit, Department of Pathology, Emory University, Atlanta, GA 30322, USA.
authors.forename: Brian A
authors.initials: BA
authors.lastname: Babbin
authors.forename: Mike G
authors.initials: MG
authors.lastname: Laukoetter
authors.forename: Porfirio
authors.initials: P
authors.lastname: Nava
authors.forename: Stefan
authors.initials: S
authors.lastname: Koch
authors.forename: Winston Y
authors.initials: WY
authors.lastname: Lee
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Capaldo
authors.forename: Eric
authors.initials: E
authors.lastname: Peatman
authors.forename: Eric A
authors.initials: EA
authors.lastname: Severson
authors.forename: Roderick J
authors.initials: RJ
authors.lastname: Flower
authors.forename: Mauro
authors.initials: M
authors.lastname: Perretti
authors.forename: Charles A
authors.initials: CA
authors.lastname: Parkos
authors.forename: Asma
authors.initials: A
authors.lastname: Nusrat
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity; D005260:Female; D020022:Genetic Predisposition to Disease; D018836:Inflammation Mediators / Q000378:metabolism / Q000502:physiology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D012720:Severity of Illness Index; D014945:Wound Healing / Q000187:drug effects / Q000276:immunology*
chemical_list: D017305:Annexin A1; D018836:Inflammation Mediators; D016264:Dextran Sulfate
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