title: Identification of postentry restrictions to Mason-Pfizer monkey virus infection in New World monkey cells.
abstract: TRIM5alpha has been shown to be a major postentry determinant of the host range for gammaretroviruses and lentiviruses and, more recently, spumaviruses. However, the restrictive potential of TRIM5alpha against other retroviruses has been largely unexplored. We sought to determine whether or not Mason-Pfizer monkey virus (M-PMV), a prototype betaretrovirus isolated from rhesus macaques, was sensitive to restriction by TRIM5alpha. Cell lines from both Old World and New World primate species were screened for their susceptibility to infection by vesicular stomatitis virus G protein pseudotyped M-PMV. All of the cell lines tested that were established from Old World primates were found to be susceptible to M-PMV infection. However, fibroblasts established from three New World monkey species specifically resisted infection by this virus. Exogenously expressing TRIM5alpha from either tamarin or squirrel monkeys in permissive cell lines resulted in a block to M-PMV infection. Restriction in the resistant cell line of spider monkey origin was determined to occur at a postentry stage. However, spider monkey TRIM5alpha expression in permissive cells failed to restrict M-PMV infection, and interference with endogenous TRIM5alpha in the spider monkey fibroblasts failed to relieve the block to infectivity. Our results demonstrate that TRIM5alpha specificity extends to betaretroviruses and suggest that New World monkeys have evolved additional mechanisms to restrict the infection of at least one primate betaretrovirus.
authors.affiliation: Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, Georgia 30329, USA.
authors.forename: William E
authors.initials: WE
authors.lastname: Diehl
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Stansell
authors.forename: Shari M
authors.initials: SM
authors.lastname: Kaiser
authors.forename: Michael
authors.initials: M
authors.lastname: Emerman
authors.forename: Eric
authors.initials: E
authors.lastname: Hunter
mesh_terms: D000818:Animals; D000090103:Antiviral Restriction Factors; D002352:Carrier Proteins / Q000276:immunology; D002460:Cell Line; D002520:Cercopithecidae; D006801:Humans; D016093:Mason-Pfizer monkey virus / Q000254:growth & development* / Q000276:immunology* / Q000502:physiology; D051078:Platyrrhini; D011506:Proteins / Q000276:immunology*; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; D053586:Virus Internalization; D014779:Virus Replication
chemical_list: D000090103:Antiviral Restriction Factors; D002352:Carrier Proteins; D011506:Proteins; D000070584:Tripartite Motif Proteins; C477346:TRIM5 protein, human; C482587:TRIM5(alpha) protein, rhesus monkey; D044767:Ubiquitin-Protein Ligases
references.citation: J Virol. 2005 Oct;79(19):12507-14
references.pmid: 16160178
references.citation: Nature. 2004 Jul 29;430(6999):569-73
references.pmid: 15243629
references.citation: J Gen Virol. 1983 Oct;64 (Pt 10):2323-7
references.pmid: 6311958
references.citation: Methods Mol Biol. 2003;229:43-55
references.pmid: 12824620
references.citation: PLoS Biol. 2005 Apr;3(4):e110
references.pmid: 15737067
references.citation: Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13324-8
references.pmid: 15326303
references.citation: J Virol. 2005 Mar;79(5):3139-45
references.pmid: 15709033
references.citation: J Virol. 2003 Jan;77(1):726-31
references.pmid: 12477877
references.citation: Science. 1984 Mar 9;223(4640):1083-6
references.pmid: 6695196
references.citation: Gene Ther. 2000 Jun;7(11):910-3
references.pmid: 10849549
references.citation: Bacteriol Rev. 1952 Sep;16(3):179-204
references.pmid: 12987386
references.citation: Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8033-7
references.pmid: 8396259
references.citation: Nucleic Acids Res. 1987 Oct 26;15(20):8125-48
references.pmid: 3313277
references.citation: J Virol. 2005 Nov;79(22):14446-50
references.pmid: 16254380
references.citation: J Virol. 2005 Sep;79(18):11580-7
references.pmid: 16140735
references.citation: J Virol. 2006 Sep;80(17):8554-65
references.pmid: 16912305
references.citation: J Virol. 2008 Jun;82(11):5429-39
references.pmid: 18367529
references.citation: J Virol. 2004 May;78(10):5423-37
references.pmid: 15113921
references.citation: Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10774-9
references.pmid: 15249685
references.citation: Nat New Biol. 1972 Jan 5;235(53):3-6
references.pmid: 4111969
references.citation: J Virol. 2006 Oct;80(19):9754-60
references.pmid: 16973579
references.citation: Nature. 2007 Mar 29;446(7135):507-12
references.pmid: 17392779
references.citation: J Virol. 2000 Aug;74(16):7422-30
references.pmid: 10906195
references.citation: Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14764-9
references.pmid: 9405687
references.citation: Proc Natl Acad Sci U S A. 1975 Nov;72(11):4635-9
references.pmid: 172912
references.citation: J Virol. 2000 Nov;74(22):10589-99
references.pmid: 11044103
references.citation: J Virol. 2004 Jun;78(11):5670-8
references.pmid: 15140964
references.citation: J Virol. 2006 Feb;80(3):1367-75
references.pmid: 16415014
references.citation: Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5514-9
references.pmid: 16540544
references.citation: Mol Cell Biol. 1988 May;8(5):2159-65
references.pmid: 2455217
references.citation: Nature. 1974 Mar 1;248(5443):17-20
references.pmid: 4131937
references.citation: J Gen Virol. 1995 Nov;76 ( Pt 11):2723-30
references.pmid: 7595379
references.citation: Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10780-5
references.pmid: 15249687
references.citation: Science. 1994 Jul 29;265(5172):666-9
references.pmid: 8036514
references.citation: Proc Natl Acad Sci U S A. 2005 Feb 22;102(8):2832-7
references.pmid: 15689398
references.citation: J Natl Cancer Inst. 1971 Nov;47(5):1115-20
references.pmid: 5001258
references.citation: AIDS Rev. 2007 Apr-Jun;9(2):114-25
references.pmid: 17694678
references.citation: Science. 2006 Oct 6;314(5796):95
references.pmid: 17023652
references.citation: Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10786-91
references.pmid: 15249690
references.citation: J Virol. 2007 Sep;81(17):8977-88
references.pmid: 17596311
references.citation: Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1668-72
references.pmid: 14757818
references.citation: J Invertebr Pathol. 1968 Sep;11(3):406-28
references.pmid: 4302140
references.citation: Virology. 1978 Jan;84(1):189-94
references.pmid: 74122
references.citation: J Virol. 1977 Aug;23(2):294-301
references.pmid: 69724
references.citation: J Virol Methods. 2008 Aug;151(2):249-256
references.pmid: 18524394
references.citation: Nucleic Acids Res. 2007;35(15):4964-76
references.pmid: 17636050
references.citation: Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11827-32
references.pmid: 15280539
references.citation: Retrovirology. 2006 Oct 12;3:70
references.pmid: 17038183
references.citation: Cancer Res. 1970 Aug;30(8):2081-6
references.pmid: 4195910
references.citation: Curr Biol. 2005 Jan 11;15(1):73-8
references.pmid: 15649369
references.citation: PLoS Pathog. 2007 Jan;3(1):e10
references.pmid: 17257061
references.citation: J Virol. 2005 Apr;79(7):3930-7
references.pmid: 15767395
references.citation: Nature. 2004 Feb 26;427(6977):848-53
references.pmid: 14985764
references.citation: PLoS Pathog. 2007 Dec;3(12):e197
references.pmid: 18159944
references.citation: Proc Natl Acad Sci U S A. 2006 Dec 12;103(50):19134-9
references.pmid: 17142324
references.citation: Proc Natl Acad Sci U S A. 1978 Feb;75(2):1004-8
references.pmid: 76312
references.citation: Science. 1936 Aug 14;84(2172):162
references.pmid: 17793252
references.citation: Curr Biol. 1999 Aug 26;9(16):861-8
references.pmid: 10469592
references.citation: Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11914-9
references.pmid: 12154227
references.citation: Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15266-71
references.pmid: 8986799
references.citation: Science. 2007 Jun 22;316(5832):1756-8
references.pmid: 17588933
references.citation: J Natl Cancer Inst. 1971 Oct;47(4):881-9
references.pmid: 4328953
references.citation: J Virol. 1968 Sep;2(9):953-4
references.pmid: 4302192
references.citation: Curr Biol. 2001 Oct 2;11(19):1531-5
references.pmid: 11591322
references.citation: Virology. 2005 Dec 20;343(2):201-11
references.pmid: 16183097
references.citation: J Virol. 2005 May;79(10):6111-21
references.pmid: 15857996
references.citation: Science. 1977 Jan 21;195(4275):289-92
references.pmid: 63993
references.citation: Retrovirology. 2005 Jun 20;2:40
references.pmid: 15967037
references.citation: Mol Ther. 2002 May;5(5 Pt 1):555-65
references.pmid: 11991746