title: A new terrein glucoside, a novel inhibitor of angiogenin secretion in tumor angiogenesis.
abstract: Angiogenesis is a critical step for the tumor therapy. Many angiogenic factors are involved in the tumor angiogenesis. In the course of our screening for inhibitors of angiogenin secretion, one of angiogenic factors, we have isolated a new terrein glucoside (1) and terrein (2) from the fermentation broth of fungal strain Aspergillus sp. PF1381. The structure and absolute stereochemistry of 1 was determined to be (4S,5R)-5-[(alpha-D-glucopyranosyl)oxy]-4-hydroxy-3-(E-1-propenyl)-2-cyclopenten-1-one on the basis of spectral and enzymatic analyses. Compounds 1 and 2 equally inhibited angiogenin secretion from androgen-dependent prostate cancer cells, LNCaP-CR, with IC50 values of 13 microM. However, both compounds did not affect VEGF secretion, another angiogenic factor. Furthermore, both compounds inhibited tube formation of human umbilical vein endothelial cells (HUVEC). These results suggested that 1 and 2 act as angiogenesis inhibitors through the inhibition of angiogenin secretion.
authors.affiliation: Drug Development Unit, Numazu Bio-Medical Research Institute, Microbial Chemistry Research Center, Shizuoka, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Arakawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Someno
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawada
authors.forename: Daishiro
authors.initials: D
authors.lastname: Ikeda
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism; D045744:Cell Line, Tumor; D003470:Culture Media / Q000737:chemistry; D003517:Cyclopentanes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D042783:Endothelial Cells; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D012259:Ribonuclease, Pancreatic / Q000037:antagonists & inhibitors* / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D003517:Cyclopentanes; D005960:Glucosides; C439087:terrein; C046366:angiogenin; D012259:Ribonuclease, Pancreatic