title: Experimental infection of eastern gray squirrels (Sciurus carolinensis) with West Nile virus.
abstract: Eastern gray squirrels (Sciurus carolinensis) have shown high West Nile virus (WNV) seroprevalence, and WNV infection has been suggested as a cause of morbidity and mortality in this species. We experimentally infected nine eastern gray squirrels with WNV to determine the clinical effects of infection and to assess their potential role as amplifying hosts. We observed no morbidity or mortality attributable to WNV infection, but lesions were apparent in several organs. We detected mean viremias of 10(5.1) and 10(4.8) plaque-forming units (PFU)/mL on days 3 and 4 post-infection (DPI) and estimated that approximately 2.1% of Culex pipiens feeding on squirrels during 1-5 DPI would become infectious. Thus, S. carolinensis are unlikely to be important amplifying hosts and may instead dampen the intensity of transmission in most host communities. The low viremias and lack of mortality observed in S. carolinensis suggest that they may be useful as sentinels of spillover from the enzootic amplification cycle.
authors.affiliation: Department of Ecology, Evolution and Environmental Biology, Columbia University, New York, NY 10027, USA. ag2112@caa.columbia.edu
authors.forename: Andrés
authors.initials: A
authors.lastname: Gómez
authors.forename: Laura D
authors.initials: LD
authors.lastname: Kramer
authors.forename: Alan P
authors.initials: AP
authors.lastname: Dupuis
authors.forename: A Marm
authors.initials: AM
authors.lastname: Kilpatrick
authors.forename: Lauren J
authors.initials: LJ
authors.lastname: Davis
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Jones
authors.forename: Peter
authors.initials: P
authors.lastname: Daszak
authors.forename: A Alonso
authors.initials: AA
authors.lastname: Aguirre
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D012376:Rodent Diseases / Q000635:transmission* / Q000821:virology; D012589:Sciuridae; D014766:Viremia; D014901:West Nile Fever / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D014902:West Nile virus / Q000472:pathogenicity
references.citation: Curr Top Microbiol Immunol. 2002;267:271-308
references.pmid: 12082994
references.citation: Am J Trop Med Hyg. 2007 Jan;76(1):173-9
references.pmid: 17255248
references.citation: Am J Trop Med Hyg. 2006 Oct;75(4):697-701
references.pmid: 17038697
references.citation: J Gen Virol. 1989 Jan;70 ( Pt 1):37-43
references.pmid: 2543738
references.citation: Emerg Infect Dis. 2002 Apr;8(4):380-6
references.pmid: 11971771
references.citation: J Virol Methods. 2006 Jun;134(1-2):183-9
references.pmid: 16510196
references.citation: Adv Virus Res. 2003;61:185-234
references.pmid: 14714433
references.citation: Am J Trop Med Hyg. 2005 Aug;73(2):467-9
references.pmid: 16103624
references.citation: Vet Pathol. 2003 Nov;40(6):703-7
references.pmid: 14608026
references.citation: Am J Trop Med Hyg. 2006 Dec;75(6):1232-3
references.pmid: 17172398
references.citation: Emerg Infect Dis. 2007 Dec;13(12):1912-4
references.pmid: 18258045
references.citation: Am J Trop Med Hyg. 2006 Aug;75(2):337-45
references.pmid: 16896145
references.citation: Emerg Infect Dis. 2001 Jul-Aug;7(4):714-21
references.pmid: 11585537
references.citation: Vector Borne Zoonotic Dis. 2004 Winter;4(4):360-78
references.pmid: 15682518
references.citation: Proc Biol Sci. 2006 Sep 22;273(1599):2327-33
references.pmid: 16928635
references.citation: Vet Pathol. 2006 Jan;43(1):58-61
references.pmid: 16407488
references.citation: J Vet Diagn Invest. 2004 May;16(3):186-90
references.pmid: 15152831
references.citation: Vet Pathol. 2001 Jul;38(4):414-21
references.pmid: 11467475
references.citation: Am J Trop Med Hyg. 2005 May;72(5):622-30
references.pmid: 15891139
references.citation: Vector Borne Zoonotic Dis. 2005 Winter;5(4):342-50
references.pmid: 16417430
references.citation: Pediatr Ann. 2007 Jul;36(7):414-22
references.pmid: 17691625
references.citation: PLoS Pathog. 2007 Sep 14;3(9):1262-70
references.pmid: 17941708
references.citation: Emerg Infect Dis. 2008 Jun;14(6):962-5
references.pmid: 18507915
references.citation: Emerg Infect Dis. 2005 Mar;11(3):425-9
references.pmid: 15757558
references.citation: Emerg Infect Dis. 2007 Jun;13(6):831-7
references.pmid: 17553220
references.citation: J Med Entomol. 2005 May;42(3):367-75
references.pmid: 15962789
references.citation: Emerg Infect Dis. 2001 Jul-Aug;7(4):736-8
references.pmid: 11585540
references.citation: Am J Trop Med Hyg. 2007 May;76(5):810-3
references.pmid: 17488896
references.citation: Emerg Infect Dis. 2003 Oct;9(10):1303-6
references.pmid: 14609468
references.citation: Emerg Infect Dis. 2006 Mar;12(3):468-74
references.pmid: 16704786
references.citation: Vector Borne Zoonotic Dis. 2005 Spring;5(1):40-7
references.pmid: 15815148
references.citation: Arch Virol. 2005 Jun;150(6):1249-56
references.pmid: 15770358
references.citation: Emerg Infect Dis. 2004 Jan;10(1):82-6
references.pmid: 15078601
references.citation: Emerg Infect Dis. 2003 Mar;9(3):311-22
references.pmid: 12643825
references.citation: Emerg Infect Dis. 2002 Sep;8(9):979-82
references.pmid: 12194778
references.citation: Vector Borne Zoonotic Dis. 2005 Fall;5(3):288-92
references.pmid: 16187899
references.citation: Hum Pathol. 2000 May;31(5):527-31
references.pmid: 10836291