Effect of claudins 6 and 9 on paracellular permeability in MDCK II cells.
The neonatal proximal tubule has a lower permeability to chloride, higher resistance, and higher relative sodium-to-chloride permeability (P(Na)/P(Cl)) than the adult tubule, which may be due to maturational changes in the tight junction. Claudins are tight-junction proteins between epithelial cells that determine paracellular permeability characteristics of epithelia. We have previously described the presence of two claudin isoforms, claudins 6 and 9, in the neonatal proximal tubule and subsequent reduction of these claudins during postnatal maturation. The question is whether changes in claudin expression are related to changes in functional characteristics in the neonatal tubule. We transfected claudins 6 and 9 into Madin-Darby canine kidney II (MDCK II) cells and performed electrophysiological studies to determine the resultant changes in physiological characteristics of the cells. Expression of claudins 6 and 9 resulted in an increased transepithelial resistance, decreased chloride permeability, and decreased P(Na)/P(Cl) and P(HCO3)/P(Cl). These findings constitute the first characterization of the permeability characteristics of claudins 6 and 9 in a cell model and may explain why the neonatal proximal tubule has lower permeability to chloride and higher resistance than the adult proximal tubule.
Sas
David
D
Dept. of Pediatrics, U.T. Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235, USA.
Hu
Mingchang
M
Moe
Orson W
OW
Baum
Michel
M
D000465:Algorithms; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism; D057167:Claudins; D004285:Dogs; D004594:Electrophysiology; D004847:Epithelial Cells / Q000187:drug effects; D007150:Immunohistochemistry; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism; D008565:Membrane Proteins / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D012996:Solutions; D014162:Transfection
C524757:CLDN9 protein, human; D002712:Chlorides; D057167:Claudins; D008565:Membrane Proteins; D012996:Solutions; C122101:claudin 6
J Clin Invest. 1984 Jul;74(1):205-11
6736248
Kidney Int. 1998 May;53(5):1254-8
9573540
J Clin Invest. 2008 Feb;118(2):619-28
18188451
Am J Physiol. 1999 Jan;276(1):C102-8
9886925
J Biol Chem. 2003 May 9;278(19):17350-9
12615928
J Membr Biol. 2007 Feb;215(2-3):147-59
17516019
Am J Physiol. 1997 Aug;273(2 Pt 2):F179-92
9277578
Curr Opin Nephrol Hypertens. 2007 Sep;16(5):459-64
17693762
Am J Physiol Renal Physiol. 2003 Dec;285(6):F1078-84
13129853
Pflugers Arch. 1986;407 Suppl 2:S156-9
3822761
Am J Physiol Renal Physiol. 2002 Sep;283(3):F525-31
12167604
Pediatr Nephrol. 2008 Feb;23(2):185-94
17684771
J Clin Invest. 1987 Jan;79(1):32-8
3793930
Am J Physiol. 1999 Jun;276(6):R1792-7
10362761
Am J Physiol Cell Physiol. 2003 Jun;284(6):C1346-54
12700140
J Cell Sci. 2005 Jun 15;118(Pt 12):2683-93
15928046
Anal Biochem. 1976 May 7;72:248-54
942051
J Biol Chem. 2002 May 31;277(22):19665-72
11880379
J Biol Chem. 2006 Nov 24;281(47):36117-23
17018523
J Virol. 2007 Nov;81(22):12465-71
17804490
Am J Physiol Renal Physiol. 2006 Dec;291(6):F1132-41
16774906
Am J Physiol Renal Physiol. 2004 Mar;286(3):F477-82
14644749
Am J Physiol Renal Physiol. 2006 Dec;291(6):F1288-99
16804102
J Clin Invest. 2001 May;107(10):1319-27
11375422
Kidney Int. 2001 Jul;60(1):173-81
11422749
Am J Physiol Renal Physiol. 2000 Apr;278(4):F596-602
10751220
Mol Cell Biol. 2004 Oct;24(19):8408-17
15367662
Am J Physiol. 1994 Sep;267(3 Pt 2):F437-42
8092257
J Virol. 2008 Apr;82(7):3555-60
18234789
Am J Physiol Regul Integr Comp Physiol. 2005 Dec;289(6):R1659-64
16051720
Pediatr Res. 1996 Feb;39(2):308-12
8825805
Am J Physiol Renal Physiol. 2008 Jan;294(1):F198-204
17977906
J Cell Sci. 2004 May 15;117(Pt 12):2435-47
15159449
J Cell Biol. 2001 Apr 16;153(2):263-72
11309408
Biophys J. 2003 Mar;84(3):1660-73
12609869
Am J Physiol Cell Physiol. 2002 Jul;283(1):C142-7
12055082
Science. 1999 Jul 2;285(5424):103-6
10390358
Histochem Cell Biol. 2008 Jul;130(1):55-70
18415116
Am J Physiol. 1983 May;244(5):F461-71
6303131