An immunochemical marker for goldfish Mauthner cells.
The Mauthner (M-) cells are pair of large reticulospinal neurons that mediate tail-flip escape responses in fish. Exploring the co-localization of scaffold and gap junction proteins at mixed (electrical and chemical) synapses, we found that the use of a particular antibody against the scaffold protein Zonula Occludens-2 (ZO-2) resulted in labeling of these cells. We show here that this staining is restricted to the Mauthner cell and evenly distributed along its dendrites and axon, also prominent in small dendritic and axonal processes. Because the observed labeling is non-specific, we suggest that the antibody might recognize a soluble protein that is primarily expressed in the Mauthner cells. While the identity of this protein is presently unknown, the use of this antibody should facilitate the identification of the Mauthner cell and its fine processes during anatomical and immunohistochemical studies, which otherwise require intracellular injection of tracer molecules during electrophysiological recordings.
Flores
Carmen E
CE
Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Ene
Smaranda
S
Pereda
Alberto E
AE
D000818:Animals; D003712:Dendrites / Q000378:metabolism; D006054:Goldfish / Q000033:anatomy & histology*; D007120:Immunochemistry / Q000379:methods; D008526:Medulla Oblongata / Q000166:cytology*; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods; D008959:Models, Neurological; D009046:Motor Neurons / Q000378:metabolism* / Q000648:ultrastructure; D062827:Zonula Occludens-2 Protein
D008565:Membrane Proteins; D062827:Zonula Occludens-2 Protein
Development. 1992 Sep;116(1):201-11
1483388
Brain Res Brain Res Rev. 2004 Dec;47(1-3):227-44
15572174
J Biochem. 1999 Mar;125(3):443-8
10050030
J Histochem Cytochem. 1996 Oct;44(10):1167-71
8813082
Eur J Neurosci. 2004 Apr;19(8):2132-46
15090040
J Comp Neurol. 1987 Jan 8;255(2):272-82
3819017
Neuron. 2005 Jul 7;47(1):13-28
15996545
Development. 2001 Jun;128(11):2131-42
11493534
Biochim Biophys Acta. 2004 Mar 23;1662(1-2):22-41
15033577
J Exp Biol. 2006 Dec;209(Pt 23):4788-801
17114411
J Neurosci. 2003 Sep 3;23(22):8159-66
12954879
Gene Expr Patterns. 2007 Aug;7(7):767-76
17632043
Ann N Y Acad Sci. 1989;563:11-33
2672948
Neuroscience. 2006 Jun 30;140(2):433-51
16650609
J Neurosci. 1995 Sep;15(9):5943-55
7666179
J Neurosci. 2003 Aug 20;23(20):7489-503
12930787
Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12545-50
18719117
J Cell Biol. 1999 Dec 13;147(6):1351-63
10601346
Neuroscience. 1991;41(1):277-85
2057063
Eur J Neurosci. 1998 Mar;10(3):1202-8
9753189
J Comp Neurol. 1994 Jan 1;339(1):76-90
8106663