Growing bacteria in liquid media.
The procedure for inoculating overnight (starter) cultures of E. coli from a single colony is described along with considerations for growing larger cultures. Also included are two methods for monitoring cell growth using a spectrophotometer or a hemacytometer.
Lech
K
K
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Brent
R
R
D001431:Bacteriological Techniques; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D012996:Solutions; D013053:Spectrophotometry / Q000379:methods
D003470:Culture Media; D012996:Solutions