title: Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP.
abstract: The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.
authors.affiliation: Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, USA.
authors.forename: Julie S
authors.initials: JS
authors.lastname: Biteen
authors.forename: Michael A
authors.initials: MA
authors.lastname: Thompson
authors.forename: Nicole K
authors.initials: NK
authors.lastname: Tselentis
authors.forename: Grant R
authors.initials: GR
authors.lastname: Bowman
authors.forename: Lucy
authors.initials: L
authors.lastname: Shapiro
authors.forename: W E
authors.initials: WE
authors.lastname: Moerner
mesh_terms: D016935:Caulobacter crescentus / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D017319:Photosensitizing Agents / Q000032:analysis* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D008164:Luminescent Proteins; D017319:Photosensitizing Agents
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