Insecticidal crystal proteins from native Bacillus thuringiensis: numerical analysis and biological activity against Spodoptera frugiperda.
Fourteen strains of Bacillus thuringiensis collected from both larvae showing disease symptoms and soil samples in northwest Argentina were characterized by insecticidal activity against Spodoptera frugiperda. First instar larvae and protein profile SDS-PAGE analysis of whole cell proteins not only allowed the differentiation of native Bacillus thuringiensis but also revealed the possibility of applying protein profile analysis in classification of toxicity patterns. Cluster analysis showed that there were two main groups. Interestingly, one of them only contained the most pathogenic native strains. The biomass-bound protease activity of native pathogenic isolates and the reference strain Bt 4D1 is also reported.
Alvarez
Analía
A
PROIMI-CONICET, Av. Belgrano y Pasaje Caseros, T4001 MVB, Tucuman, Argentina.
Pera
Licia M
LM
Loto
Flavia
F
Virla
Eduardo G
EG
Baigori
Mario D
MD
D000818:Animals; D001413:Bacillus thuringiensis / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D018533:Biomass; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000382:microbiology; D008969:Molecular Sequence Data; D009716:Numerical Analysis, Computer-Assisted; D010447:Peptide Hydrolases / Q000378:metabolism; D010572:Pest Control, Biological; D010802:Phylogeny; D012988:Soil Microbiology; D018411:Spodoptera / Q000187:drug effects*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D010447:Peptide Hydrolases