Type II hydride transferases from different microorganisms yield nitrite and diarylamines from polynitroaromatic compounds.
Homogenous preparations of XenB of Pseudomonas putida, pentaerythritol tetranitrate reductase of Enterobacter cloacae, and N-ethylmaleimide reductase of Escherichia coli, all type II hydride transferases of the Old Yellow Enzyme family of flavoproteins, are shown to reduce the polynitroaromatic compound 2,4,6-trinitrotoluene (TNT). The reduction of this compound yields hydroxylaminodinitrotoluenes and Meisenheimer dihydride complexes, which, upon condensation, yield stoichiometric amounts of nitrite and diarylamines, implying that type II hydride transferases are responsible for TNT denitration, a process with important environmental implications for TNT remediation.
van Dillewijn
Pieter
P
Departamento de Protección Ambiental, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain.
Wittich
Rolf-Michael
RM
Caballero
Antonio
A
Ramos
Juan-Luis
JL
D000588:Amines / Q000378:metabolism*; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D016972:Enterobacter cloacae / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D005420:Flavoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D009603:Nitroso Compounds / Q000378:metabolism*; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology; D014166:Transferases / Q000378:metabolism*
D000588:Amines; D001426:Bacterial Proteins; D005420:Flavoproteins; D006841:Hydrocarbons, Aromatic; D009573:Nitrites; D009603:Nitroso Compounds; C122135:XenB protein, Pseudomonas; D010088:Oxidoreductases; C040723:alpha,beta-ketoalkene reductase; C098939:pentaerythritol tetranitrate reductase; D014166:Transferases
Environ Microbiol. 2007 Jun;9(6):1535-40
17504490
Environ Sci Technol. 2007 Feb 15;41(4):1378-83
17593745
Environ Microbiol. 2005 Aug;7(8):1211-9
16011758
Appl Environ Microbiol. 1998 Jan;64(1):246-52
16349484
Appl Environ Microbiol. 2003 Mar;69(3):1542-7
12620841
Appl Environ Microbiol. 1998 Aug;64(8):2864-8
9687442
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
9254694
Appl Environ Microbiol. 2007 Dec;73(24):7898-905
17933928
Appl Environ Microbiol. 2004 Jun;70(6):3566-74
15184158
Curr Opin Microbiol. 2002 Jun;5(3):282-7
12057682
Appl Environ Microbiol. 2008 Nov;74(21):6703-8
18791012
Environ Microbiol. 2006 Jul;8(7):1306-10
16817939
Environ Sci Technol. 2008 Feb 1;42(3):734-9
18323095
Appl Microbiol Biotechnol. 2007 Aug;76(2):267-77
17534614
Microbiol Mol Biol Rev. 2001 Sep;65(3):335-52, table of contents
11527999
Appl Environ Microbiol. 2006 Dec;72(12):7945-8
17012591
Environ Toxicol Chem. 2004 Aug;23(8):1852-60
15352472
Microbiology (Reading). 2002 Jun;148(Pt 6):1607-1614
12055282
Appl Environ Microbiol. 2000 Nov;66(11):4742-50
11055918