title: Properties of mutants of Synechocystis sp. strain PCC 6803 lacking inorganic carbon sequestration systems.
abstract: A mutant (Delta5) of Synechocystis sp. strain PCC 6803 constructed by inactivating five inorganic carbon sequestration systems did not take up CO(2) or HCO(3)(-) and was unable to grow in air with or without glucose. The Delta4 mutant in which BicA is the only active inorganic carbon sequestration system showed low activity of HCO(3)(-) uptake and grew under these conditions but more slowly than the wild-type strain. The Delta5 mutant required 1.7% CO(2) to attain half the maximal growth rate. Electron transport activity of the mutants was strongly inhibited under high light intensities, with the Delta5 mutant more susceptible to high light than the Delta4 mutant. The results implicated the significance of carbon sequestration in dissipating excess light energy.
authors.affiliation: National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Graduate courses of the Chinese Academy of Sciences, Shanghai, 200032, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Gábor
authors.initials: G
authors.lastname: Bernát
authors.forename: Abhay
authors.initials: A
authors.lastname: Singh
authors.forename: Hualing
authors.initials: H
authors.lastname: Mi
authors.forename: Matthias
authors.initials: M
authors.lastname: Rögner
authors.forename: Himadri B
authors.initials: HB
authors.lastname: Pakrasi
authors.forename: Teruo
authors.initials: T
authors.lastname: Ogawa
mesh_terms: D001639:Bicarbonates / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004579:Electron Transport; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008027:Light; D016296:Mutagenesis; D009154:Mutation; D046939:Synechocystis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D001639:Bicarbonates; D004269:DNA, Bacterial; D002245:Carbon Dioxide; D005947:Glucose