title: RNA containing pyrrolocytidine base analogs: good binding affinity and fluorescence that responds to hybridization.
abstract: 6-Phenylpyrrolocytidine and 6-methoxymethylene-pyrrolocytidine are base-modified nucleosides with remarkable fluorescence properties. When incorporated into RNA, these analogs enhance binding affinity towards RNA and DNA targets with a concomitant change in their fluorescence upon duplex formation. The fluorescence response depends on the nature of the 6-substituent and the sequence position of the modified nucleoside. The fluorescence response of these structurally conservative, well-tolerated fluorescent nucleosides may be exploited as probes in the study of nucleic acid processing enzymes.
authors.affiliation: Department of Chemistry, McGill University, Montreal, QC, Canada.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Wahba
authors.forename: Masad J
authors.initials: MJ
authors.lastname: Damha
authors.forename: Robert H E
authors.initials: RH
authors.lastname: Hudson
mesh_terms: D003562:Cytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D015347:RNA Probes / Q000737:chemistry*
chemical_list: C541629:6-methoxymethylenepyrrolocytidine; C541628:6-phenylpyrrolocytidine; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011758:Pyrroles; D015347:RNA Probes; D003562:Cytidine; D012313:RNA; D004247:DNA