New recurrent deletions in the PPARgamma and TP53 genes are associated with childhood myelodysplastic syndrome.
Myelodysplastic syndrome (MDS) is a rare hematological malignancy in children. It was performed FISH analysis in 19 pediatric MDS patients to investigate deletions involving the PPARgamma and TP53 genes. Significant losses in the PPARgamma gene and deletions in the tumor suppressor gene TP53 were observed in 17 and 18 cases, respectively. Using quantitative RT-PCR, it was detected PPARgamma transcript downexpression in a subset of these cases. G-banding analysis revealed 17p deletions in a small number of these cases. One MDS therapy-related patient had neither a loss of PPARgamma nor TP53. These data suggest that the PPARgamma and TP53 genes may be candidates for molecular markers in pediatric MDS, and that these potentially recurrent deletions could contribute to the identification of therapeutic approaches in primary pediatric MDS.
Silveira
Cássia G T
CG
Department of Genetics, Institute of Biosciences, Sao Paulo State University-UNESP, Botucatu, Sao Paulo, Brazil.
Oliveira
Fábio M
FM
Valera
Elvis T
ET
Ikoma
Maura R V
MR
Borgonovo
Tamara
T
Cavalli
Iglenir J
IJ
Tone
Luiz G
LG
Rogatto
Silvia R
SR
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002871:Chromosome Banding; D005260:Female; D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007621:Karyotyping; D008297:Male; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D047495:PPAR gamma; D016159:Tumor Suppressor Protein p53