[Analysis of TCR V gamma gene diversity from peripheral blood in patients with chronic benzene poisoning].
OBJECTIVE
To observe the TCR V gamma gene repertoire diversity in the peripheral blood mononuclear cells of the patients with the chronic benzene poisoning.


METHODS
Complementarity determining region 3 (CDR3) of TCR V gamma subfamily genes were amplified in 10 patients with the chronic benzene poisoning using RT-PCR. The PCR products were further analyzed by genescan to evaluate clonality of T cells. 8 healthy persons served as control.


RESULTS
All V gamma subfamilies were detected in the 8 healthy donors. (1.30 +/- 0.48) V gamma subfamilies were detected in 10 patients. The number of detectable V gamma subfamilies present in the patients with the chronic benzene poisoning was significantly lower than in the control group (P < 0.01). The most frequently used V gamma genes in clonally expanded T-cells were V gamma II.


CONCLUSIONS
Skewed distribution and clonal expansion of TCR V gamma subfamily T cells could be found in the patients with the chronic benzene poisoning. This is the first report of clonal expansion TCR V gamma T cells in patients with chronic benzene poisoning. The bias pattern of TCR V alpha T cells may be due to the immune cytotoxicity from benzene.
Li
Bo
B
Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
Liu
Wei-Wei
WW
Yu
Wei
W
D000328:Adult; D001554:Benzene / Q000506:poisoning*; D002908:Chronic Disease; D022801:Complementarity Determining Regions / Q000235:genetics; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000235:genetics* / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
D022801:Complementarity Determining Regions; D016692:Receptors, Antigen, T-Cell, gamma-delta; D001554:Benzene