title: Characterization of heterogeneous LSU rRNA profiles in Streptomyces coelicolor under different growth stages and conditions.
abstract: The Streptomyces coelicolor genome harbors six copies of divergent large subunit (LSU) rRNA genes that constitute five kinds of LSU rRNA species in a cell. We report here that each heterogeneous LSU rRNA species is differentially expressed during morphological development. However, differential expression of rRNA species was not affected by depletion of a specific nutrient such as carbon, nitrogen, or phosphate from the culture medium. Analysis of the upstream region of the rRNA operons revealed that each operon contains a different composition of conserved rRNA gene promoters, indicating that each operon is independently regulated at the transcriptional level. These findings imply the existence of a regulatory mechanism that controls the independent expression of each LSU rRNA and a possible role of different species of LSU rRNA in posttranscriptional regulation of gene expression during the life cycle of this developmentally complex microorganism.
authors.affiliation: Life Science, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu Seoul, South Korea.
authors.forename: Hyun-Lee
authors.initials: HL
authors.lastname: Kim
authors.forename: Woo-Seok
authors.initials: WS
authors.lastname: Song
authors.forename: Kyungsub
authors.initials: K
authors.lastname: Kim
authors.forename: Kangseok
authors.initials: K
authors.lastname: Lee
mesh_terms: D002244:Carbon / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012338:RNA, Ribosomal, 23S / Q000096:biosynthesis* / Q000235:genetics*; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000254:growth & development*
chemical_list: D004269:DNA, Bacterial; D010710:Phosphates; D012338:RNA, Ribosomal, 23S; D002244:Carbon; D009584:Nitrogen
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