Regulation of matrix metalloproteinase-9 expression between gingival fibroblast cells from old and young rats.
Gingival fibroblast cells (rGF) from aged rats have an age-related decline in proliferative capacity compared with young rats. We investigated G1 phase cell cycle regulation and MMP-9 expression in both young and aged rGF. G1 cell cycle protein levels and activity were significantly reduced in response to interleukin-1beta (IL-1beta) stimulation with increasing in vitro age. Tumor necrosis factor-alpha (TNF-alpha)-induced matrix metalloproteinase-9 (MMP-9) expression was also decreased in aged rGF in comparison with young rGF. Mutational analysis and gel shift assays demonstrated that the lower MMP-9 expression in aged rGF is associated with lower activities of transcription factors NF-kappaB and AP-1. These results suggest that cell cycle dysregulation and down-regulation of MMP-9 expression in rGF may play a role in gingival remodeling during in vitro aging.
Kim
Su-Jung
SJ
Department of Orthodontics, Kyung-Hee University School of Dentistry, Hoegi-Dong 1, Dongdaemoon-Ku, Seoul, Republic of Korea.
Chung
Yong-Koo
YK
Chung
Tae-Wook
TW
Kim
Jeong-Ran
JR
Moon
Sung-Kwon
SK
Kim
Cheorl-Ho
CH
Park
Young-Guk
YG
D000367:Age Factors; D000375:Aging / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D004247:DNA / Q000378:metabolism; D004252:DNA Mutational Analysis; D015536:Down-Regulation; D024202:Electrophoretic Mobility Shift Assay; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D016193:G1 Phase / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic; D005881:Gingiva / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D053583:Interleukin-1beta / Q000494:pharmacology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D016328:NF-kappa B / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051381:Rats; D017208:Rats, Wistar; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D053583:Interleukin-1beta; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D014409:Tumor Necrosis Factor-alpha; D004247:DNA; D020780:Matrix Metalloproteinase 9