title: Ultrastructural histopathology of chick embryo chorioallantoic membrane infected with an avian reovirus.
abstract: The histopathological pattern of chick embryo chorioallantoic membrane infected by an avian reovirus, Crawley strain, has been investigated by semithin sections and transmission electron microscopy of ultrathin sections. The typical virus-induced pock is made up of a crust of necrotic ectodermal cell elements, an ectodermal proliferation and a proliferated, infiltrated, and oedematous mesoderm. Large crystals of virions are present in the ectodermal cells of the pock, where the details of the viral morphogenesis are not clearly revealed. While the virus appears neither to replicate in the mesodermal elements, nor in endodermal cells, it actively replicates in macrophages inside blood vessels. This finding may account for the dissemination of the infection. The pattern of replication of virions in these cells is identical to that described in in vitro tissue cultures.
authors.affiliation: Institute of Hygiene, University of Padua, Padua, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Baroni
authors.forename: P
authors.initials: P
authors.lastname: Bertoncin
authors.forename: P N
authors.initials: PN
authors.lastname: D'Aprile
authors.forename: B
authors.initials: B
authors.lastname: Felluga