Practical solvent system selection for counter-current separation of pharmaceutical compounds.
Counter-current chromatography (CCC) is a technique that shows a lot of potential for large scale purification. Its usefulness in a "research and development" pharmaceutical environment has been investigated, and the conclusions are shown in this article. The use of CCC requires the development of an appropriate solvent system (a parameter of critical importance), a process which can be tedious. This article presents a novel strategy, combining a statistical approach and fast HPLC to generate a three-dimensional partition coefficient map and rapidly predict an optimal solvent system. This screen is performed in half a day and involves 9 experiments per solvent mixture. Test separations were performed using that screen to ensure the validity of the method.
Dubant
Stéphane
S
Chemical R&D, Pfizer Global R&D, Sandwich, Kent CT13 9NJ, UK. sdubant@gmail.com
Mathews
Ben
B
Higginson
Paul
P
Crook
Robert
R
Snowden
Martin
M
Mitchell
John
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry*
D004364:Pharmaceutical Preparations; D012997:Solvents