The intra-nucleus integration of mitochondrial DNA (mtDNA)in cervical mucosa cells and its relation with c-myc expression.
OBJECTIVE
To explore the relationship between the integration of mitochondrial DNA(mtDNA) in the nuclei of cervical epithelium cells and the expression of c-myc.


METHODS
The expression of c-myc protein was measured by immunohistochemical test in 40 cases of the uterine cervix cancer, 30 cases of cervical intraepithelial neoplasia (CIN) and 30 cases of normal cervical epithelium; the sequence of mtDNA in the nuclei was detected by in situ hybridization technique.


RESULTS
The detection rates of mtDNA in the nuclei of cervical epithelium cells were 27.5%, 13.3% and 0% in cervical carcinoma, CIN, and normal cervical epithelium respectively. The expression rate of c-myc in cervical mucoma cells was 67% in the mtDNA sequence positive group and was significantly higher than that in the negative group (36%).


CONCLUSIONS
The integration of mtDNA into the nuclei of cervical epithelium cells may be involved in the carcinogenesis of cervical epithelium cells and the expression of c-myc might be related to the integration of mtDNA sequence into nuclei of cervical epithelium cells.
Chen
Daozhen
D
Center Laboratory, Wuxi Hospital for Matemaland Child Health Care, Affiliated Medical University of Nanjing, Wuxi, PR China. chendaozhen@163.com
Xue
Wenqun
W
Xiang
Jinying
J
D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology
D004272:DNA, Mitochondrial; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc
Oncogene. 1991 Oct;6(10):1869-74
1923509
J Mol Biol. 1989 Dec 20;210(4):703-7
2614844
Mutat Res. 1996 Jul 5;354(1):27-33
8692203
J Mol Biol. 1991 Oct 20;221(4):1223-35
1942048
J Cancer Res Clin Oncol. 2009 Feb;135(2):291-5
18584207
Mutat Res. 1992 Sep;275(3-6):227-35
1383764
Diagn Mol Pathol. 1998 Dec;7(6):324-30
10207671
Eur J Cancer. 1999 Oct;35(10):1546-50
10673985
Zhonghua Bing Li Xue Za Zhi. 2000 Feb;29(1):39-42
11866891
J Cell Biochem. 1998 Jan 1;68(1):100-9
9407318