Tissue culture cell assays used to analyze Listeria monocytogenes.
This unit describes tissue culture cell assays for analysis of the ability of Listeria monocytogenes to cause intracellular infection. It includes methods for evaluating the organism's ability to invade its host, to escape the primary vacuole formed upon invasion of host cells, to multiply within the cytosol of its host, and to spread from cell to cell without exiting the intracellular milieu. Each step can be evaluated quantitatively and qualitatively.
Marquis
Hélène
H
Cornell University, Ithaca, New York.
D000818:Animals; D001431:Bacteriological Techniques; D018938:Caco-2 Cells / Q000382:microbiology; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D005347:Fibroblasts / Q000382:microbiology*; D006801:Humans; D007739:L Cells / Q000382:microbiology; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000472:pathogenicity*; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D051381:Rats; D014774:Virulence
Bakardjiev, A.I., Stacy, B.A., Fisher, S.J., and Portnoy, D.A. 2004. Listeriosis in the pregnant guinea pig: A model of vertical transmission. Infect. Immun. 72:489-497.
Bishop, D.K. and Hinrichs, D.J. 1987. Adoptive transfer of immunity to Listeria monocytogenes: The influence of in vitro stimulation on lymphocyte subset requirements. J. Immunol. 139:2005-2009.
CDC. 1999. Multistate outbreak of listeriosis-United States, 1998-1999. JAMA 281:317-318.
Dales, S. 1962. An electron microscope study of the early association between two mammalian viruses and their hosts. J. Cell. Biol. 13:303-322.
Dussurget, O., Pizarro-Cerda, J., and Cossart, P. 2004. Molecular determinants of Listeria monocytogenes virulence. Annu. Rev. Microbiol. 58:587-610.
Fenlon, D.R. 1999. Listeria monocytogenes in the natural environment. In Listeria, listeriosis, and food safety, 2nd ed. (E. T. Ryser and E. H. Marth, eds.) pp. 21-37. Marcel Dekker, New York.
Gaillard, J.-L., Berche, P., Frehel, C., Gouin, E., and Cossart, P. 1991. Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocci. Cell 65:1127-1141.
Gaillard, J.-L., Berche, P., Mounier, J., Richard, S., and Sansonetti, P. 1987. In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2. Infect. Immun. 55:2822-2829.
Gaillard, J.-L., Jaubert, F., and Berche, P. 1996. The inlAB locus mediates the entry of Listeria monocytogenes into hepatocytes in vivo. J. Exp. Med. 183:359-369.
Gray, M.J., Zadoks, R.N., Fortes, E.D., Dogan, B., Cai, S., Chen, Y., Scott, V.N., Gombas, D.E., Boor, K.J., and Wiedmann, M. 2004. Listeria monocytogenes isolates from foods and humans form distinct but overlapping populations. Appl. Environ. Microbiol. 70:5833-5841.
Jones, S., Preiter, K., and Portnoy, D.A. 1996. Conversion of an extracellular cytolysin into a phagosome-specific lysin which supports the growth of an intracellular pathogen. Mol. Microbiol. 21:1219-1225.
Kim, H., Boor, K.J., and Marquis, H. 2004. Listeria monocytogenes σB contributes to invasion of human intestinal epithelial cells. Infect. Immun. 72:7374-7378.
Lenz, L.L. and Portnoy, D.A. 2002. Identification of a second Listeria secA gene associated with protein secretion and the rough phenotype. Mol. Microbiol. 45:1043-1056.
Marquis, H., Doshi, V., and Portnoy, D.A. 1995. The broad-range phospholipase C and a metalloprotease mediate listeriolysin O-independent escape of Listeria monocytogenes from a primary vacuole in human epithelial cells. Infect. Immun. 63:4531-4534.
Mounier, J., Ryter, A., Coquis-Rondon, M., and Sansonetti, P.J. 1990. Intracellular and cell-to-cell spread of Listeria monocytogenes involves interaction with F-actin in the enterocytelike cell line Caco-2. Infect. Immun. 58:1048-1058.
Portnoy, D.A., Chakraborty, T., Goebel, W., and Cossart, P. 1992. Molecular determinants of Listeria monocytogenes pathogenesis. Infect. Immun. 60:1263-1267.
Portnoy, D.A., Jacks, P.S., and Hinrichs, D.J. 1988. Role of hemolysin for the intracellular growth of Listeria monocytogenes. J. Exp. Med. 167:1459-1471.
Sun, A.N., Camilli, A., and Portnoy, D.A. 1990. Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread. Infect. Immun. 58:3770-3778.
Swanson, J.A. and Baer, S.C. 1995. Phagocytosis by zippers and triggers. Trends Cell Biol. 5:89-93.
Tilney, L.G., and Portnoy, D.A. 1989. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes. J. Cell. Biol. 109:1597-1608.
Vazquez-Boland, J.A., Kuhn, M., Berche, P., Chakraborty, T., Dominguez-Bernal, G., Goebel, W., Gonzalez-Zorn, B., Wehland, J., and Kreft, J. 2001. Listeria pathogenesis and molecular virulence determinants. Clin. Microbiol. Rev. 14:584-640.