Placental protein 13 (galectin-13) has decreased placental expression but increased shedding and maternal serum concentrations in patients presenting with preterm pre-eclampsia and HELLP syndrome.
Placental protein 13 (PP13) is a galectin expressed by the syncytiotrophoblast. Women who subsequently develop preterm pre-eclampsia have low first trimester maternal serum PP13 concentrations. This study revealed that third trimester maternal serum PP13 concentration increased with gestational age in normal pregnancies (p < 0.0001), and it was significantly higher in women presenting with preterm pre-eclampsia (p = 0.02) and hemolysis, elevated liver enzymes, and low platelet count (HELLP) syndrome (p = 0.01) than in preterm controls. Conversely, placental PP13 mRNA (p = 0.03) and protein, as well as cytoplasmic PP13 staining of the syncytiotrophoblast (p < 0.05) was decreased in these pathological pregnancies compared to controls. No differences in placental expression and serum concentrations of PP13 were found at term between patients with pre-eclampsia and control women. In contrast, the immunoreactivity of the syncytiotrophoblast microvillous membrane was stronger in both term and preterm pre-eclampsia and HELLP syndrome than in controls. Moreover, large syncytial cytoplasm protrusions, membrane blebs and shed microparticles strongly stained for PP13 in pre-eclampsia and HELLP syndrome. In conclusion, parallel to its decreased placental expression, an augmented membrane shedding of PP13 contributes to the increased third trimester maternal serum PP13 concentrations in women with preterm pre-eclampsia and HELLP syndrome.
Than
Nandor Gabor
NG
First Department of Obstetrics and Gynecology, Semmelweis University, 27 Baross Street, 1088, Budapest, Hungary. nthan@med.wayne.edu
Abdul Rahman
Omar
O
Magenheim
Rita
R
Nagy
Balint
B
Fule
Tibor
T
Hargitai
Beata
B
Sammar
Marei
M
Hupuczi
Petronella
P
Tarca
Adi L
AL
Szabo
Gabor
G
Kovalszky
Ilona
I
Meiri
Hamutal
H
Sziller
Istvan
I
Rigo
Janos
J
Romero
Roberto
R
Papp
Zoltan
Z
D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D037161:Galectins / Q000097:blood* / Q000378:metabolism; D017359:HELLP Syndrome / Q000097:blood*; D006801:Humans; D010920:Placenta / Q000378:metabolism / Q000473:pathology; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood* / Q000378:metabolism; D011263:Pregnancy Trimester, Third; D014327:Trophoblasts / Q000378:metabolism
D037161:Galectins; C038195:LGALS13 protein, human; D011257:Pregnancy Proteins
Clin Perinatol. 2004 Dec;31(4):807-33, vii
15519429
Mol Membr Biol. 2006 Jan-Feb;23(1):71-9
16611582
Placenta. 2004 Aug;25(7):608-22
15193867
Am J Obstet Gynecol. 2007 Jul;197(1):35.e1-7
17618748
Thromb Res. 2004;114(5-6):397-407
15507270
Placenta. 2007 Apr;28 Suppl A:S4-13
17379302
Clin Genet. 2003 Aug;64(2):96-103
12859402
Hum Reprod Update. 2006 Nov-Dec;12(6):747-55
16682385
Placenta. 1999 Nov;20(8):703-10
10527825
Protein Eng. 2001 Nov;14(11):875-80
11742106
Eur J Obstet Gynecol Reprod Biol. 2005 Feb 1;118(2):182-9
15653200
Placenta. 2006 Jan;27(1):56-61
16310038
Cells Tissues Organs. 2007;185(4):269-84
17587801
Hum Genet. 2007 Jan;120(5):607-12
17024365
Hypertens Pregnancy. 2003;22(2):143-8
12908998
J Proteome Res. 2005 Nov-Dec;4(6):2435-41
16335998
Ultrasound Obstet Gynecol. 2007 Feb;29(2):128-34
17149788
Eur J Biochem. 2004 Mar;271(6):1065-78
15009185
Am J Obstet Gynecol. 1989 Nov;161(5):1200-4
2589440
Am J Obstet Gynecol. 2008 Aug;199(2):122.e1-122.e11
18539259
Cell. 1994 Feb 25;76(4):597-8
8124704
Placenta. 2007 Feb-Mar;28(2-3):224-32
16580726
J Clin Pathol. 2004 Aug;57(8):785-92
15280396
Nat Med. 2006 Jun;12(6):642-9
16751767
J Anat. 1994 Jun;184 ( Pt 3):535-43
7928643
Obstet Gynecol. 2002 Jan;99(1):159-67
16175681
Am J Obstet Gynecol. 2004 Jun;190(6):1541-7; discussion 1547-50
15284729
Placenta. 1980 Jan-Mar;1(1):61-76
7443636
BJOG. 2006 May;113(5):580-9
16579806
Ultrasound Obstet Gynecol. 2006 Jan;27(1):13-7
16374755
Nat Genet. 2005 May;37(5):514-9
15806103
Am J Obstet Gynecol. 2002 Feb;186(2):210-3
11854637
Gynecol Obstet Invest. 2007;63(1):15-21
16864982
J Reprod Immunol. 2007 Dec;76(1-2):61-7
17482271
Pediatr Dev Pathol. 2004 May-Jun;7(3):237-49
15022063
Orv Hetil. 1991 Aug 25;132(34):1865-6, 1869-70
1881664
Eur J Hum Genet. 2001 Oct;9(10):758-64
11781687
Am J Obstet Gynecol. 1999 Feb;180(2 Pt 1):499-506
9988826
Science. 2005 Jun 10;308(5728):1592-4
15947178
Traffic. 2005 Aug;6(8):607-14
15998317
Am J Obstet Gynecol. 1996 Nov;175(5):1365-70
8942516
Mol Hum Reprod. 2007 Apr;13(4):281-5
17314117
Placenta. 2001 Nov;22(10):808-12
11718567
Am J Obstet Gynecol. 2003 Oct;189(4):1173-7
14586374
Placenta. 2006 Apr;27 Suppl A:S141-3
16542721
Arch Pathol Lab Med. 1997 May;121(5):449-76
9167599
Placenta. 2005 Apr;26 Suppl A:S31-6
15837064
Am J Obstet Gynecol. 2001 Oct;185(4):792-7
11641653
Am J Obstet Gynecol. 1998 Dec;179(6 Pt 1):1539-44
9855593
Semin Perinatol. 1999 Feb;23(1):45-57
10102170
Endocrine. 2002 Oct;19(1):103-11
12583607
Mol Reprod Dev. 2008 Jan;75(1):175-83
17546632
Pathology. 2001 May;33(2):174-86
11358051
Oncodev Biol Med. 1983;4(5):343-50
6856484
J Clin Invest. 2003 Mar;111(5):649-58
12618519
Hypertens Pregnancy. 2000;19(2):163-72
10877984
Int J Biochem Cell Biol. 2005 Jan;37(1):1-16
15381142
J Perinat Med. 2006;34(6):447-58
17140293
Lancet. 2005 Feb 26-Mar 4;365(9461):785-99
15733721
J Obstet Gynaecol. 2005 Feb;25(2):117-8
15814385