Production and structural analysis of the polysaccharide secreted by Trametes (Coriolus) versicolor ATCC 200801.
Trametes versicolor ATCC 200801 secretes 4.1 g L(-1) of exopolysaccharide (EPS) when synthetic minimal medium and low-shear bioreactor cultivation technique are used. Structural and compositional analyses by thin layer chromatography, gas chromatography-mass spectrometry, electrospray ionization tandem mass spectrometry, and nuclear magnetic resonance spectroscopy yielded predominantly glucose and small amounts of galactose, mannose, arabinose, and xylose. The main EPS is composed of beta-1,3/beta-1,6-linked D-glucose molecules which is identical with Schizophyllan but does not possess a triple helical arrangement as secondary structure. Two molar mass fractions were detected by size exclusion chromatography yielding weight-average molecular weights of 4,100 and 2.6 kDa. Protein content varies between 2-3.6% (w/w). The exopolysaccharide is different in the nature of the glycosidic linkage, composition of monosaccharides, protein content, and weight-average molecular weight compared to the well-known polysaccharopeptide (PSP) and polysaccharopeptide Krestin (PSK).
Rau
Udo
U
Institute of Biochemistry and Biotechnology, Technical University Braunschweig, Spielmannstr. 7, 38106 Braunschweig, Germany. U.Rau@tu-bs.de
Kuenz
Anja
A
Wray
Victor
V
Nimtz
Manfred
M
Wrenger
Julika
J
Cicek
Hasan
H
D001089:Arabinose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D046911:Macromolecular Substances / Q000737:chemistry* / Q000378:metabolism*; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D055454:Trametes / Q000378:metabolism*; D014994:Xylose / Q000032:analysis
D046911:Macromolecular Substances; D011134:Polysaccharides; D011506:Proteins; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D008358:Mannose; D005690:Galactose