[Immune response induced by human cytomegalovirus DNA vaccine].
Human cytomegalovirus is an important pathogen which widely infects human. In this study, an eukaryotic expression vector containing human cytomegalovirus gB680 and pp65m genes was injected into BALB/c mice to explore the immune response. Firstly, the recombinant plasmid pVAX1/gB680+pp65m and plasmid pVAX1 were transfected into COS-7 cells respectively and the transiently expressed product was detected by RT-PCR and Western blot. The maxiprepared plasmid pVAX1/gB680 + pp65m and pVAX1 by alkaline lysis with SDS and purified by Sepharose 4FF column chromatography were then used to immunize BALB/c mice and the humoral and cellular immune responses were determined. Recombinant plasmid pVAX1/gB680+pp65m could be expressed in COS-7 cells, and could induce antibodies and cellular immune responses in BALB/c mice.
Li
Meng
M
Department of Clinical Medicine, Weifang Medical College, Weifang 261042, China.
Shao
Li-jun
LJ
Guo
Li-jun
LJ
Wang
Xuan-jun
XJ
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D019556:COS Cells; D002522:Chlorocebus aethiops; D022482:Cytomegalovirus Vaccines / Q000276:immunology*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000276:immunology*
D000914:Antibodies, Viral; D022482:Cytomegalovirus Vaccines; D019444:Vaccines, DNA