Use of potato virus X (PVX)-based vectors for gene expression and virus-induced gene silencing (VIGS).
Potato virus X-based vectors are a well established system for rapid in planta studies. The vectors can be used for expression of proteins in plants and to down-regulate genes through virus-induced gene silencing. The development of binary-based vectors for Agrobacterium delivery makes this system well suited to high-throughput studies. Protocols are given for establishing infections to achieve expression and VIGS through in vitro transcription and Agrobacterium delivery to glasshouse and in vitro-grown plant material.
Lacomme
Christophe
C
Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee, United Kingdom.
Chapman
Sean
S
D015870:Gene Expression; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D018515:Plant Leaves / Q000821:virology; D035281:Plant Tubers / Q000821:virology; D010944:Plants / Q000821:virology; D017863:Potexvirus / Q000235:genetics* / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic