title: Immunophenotyping.
abstract: This unit presents basic techniques for immunophenotyping by flow cytometry, direct using a conjugated monoclonal antibody and indirect using an unconjugated primary antibody followed by a conjugated secondary antibody. Combinations of these methods are described for two-, three-, and four-color staining. Analysis of data acquired from cells stained by these procedures is detailed. A procedure is given for the detection of the location of nonviable cells so that they can be gated out of the analysis.
authors.affiliation: Roswell Park Cancer Institute, Buffalo, New York, USA.
authors.forename: C C
authors.initials: CC
authors.lastname: Stewart
authors.forename: S J
authors.initials: SJ
authors.lastname: Stewart
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes