[Effect of treatment with anti-receptor for advanced glycation end products antibody on multiple organ dysfunction and mortality in rats after severe thermal injury].
OBJECTIVE
To observe the effect of treatment with anti-receptor for advanced glycation end products (RAGE) antibody on multiple organ dysfunction and mortality in rats after severe burn injury, and investigate the mechanisms underlying its protective effect.


METHODS
Male Wistar rats were subjected to 30% full thickness scald injury followed by delayed resuscitation (40 ml/kg lactated Ringer's solution resuscitation administered intraperitoneally 6 hours after the injury). One hundred and thirty rats were randomly divided into sham scald group (n = 10), scald group (n = 60) and anti-RAGE antibody treatment group (n = 60). Anti-RAGE antibody (1 mg/kg) was given via dorsal penile vein at 6 and 24 hours after burn injury. Survival rate of rats in each group was recorded daily up to 7 days after the injury. Seventy-two rats were randomly divided into sham scald group, scald group and anti-RAGE antibody treatment group (n = 24). Rats in each group were further divided into four subgroups of 6 rats each. They were sacrificed on postburn days 1, 3, 5 and 7 respectively, and blood samples were obtained to determine parameters reflecting hepatic, renal as well as cardiac functions.


RESULTS
Compared with the sham scald group, levels of serum alanine amino transferase (ALT), aspartate amino transferase (AST), creatinine (Cr), blood urea nitrogen (BUN), and MB isoenzyme of creatine kinase (CK-MB) were significantly elevated during postburn days 1-7 (P < 0.05 or P < 0.01), and serum levels of ALT, AST, Cr, and BUN peaked on postburn day 3. Treatment with anti-RAGE antibody, however, the levels of above parameters were markedly lowered compared with the scald group, and serum AST levels were significantly lowered during whole observational period, while other parameters were markedly improved during postburn days 1-5 (P < 0.05 or P < 0.01). Moreover, survival rates of the scald rats treated with anti-RAGE antibody were significantly raised compared with the scald rats daily up to 7 days after burns (P < 0.05 or P < 0.01).


CONCLUSIONS
Anti-RAGE antibody could obviously improve the survival in rats with burn injury with delayed resuscitation, and it seems to be able to prevent the development of multiple organ dysfunction secondary to severe burns.
Zhang
Xiao-tian
XT
Burns Institute, First Hospital Affiliated to The PLA General Hospital, Beijing 100037, China.
Yao
Yong-ming
YM
Huang
Li-feng
LF
Yu
Yan
Y
D000818:Animals; D002056:Burns / Q000188:drug therapy / Q000503:physiopathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors*; D006321:Heart / Q000503:physiopathology; D007668:Kidney / Q000503:physiopathology; D008099:Liver / Q000503:physiopathology; D008297:Male; D009102:Multiple Organ Failure / Q000188:drug therapy* / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012151:Resuscitation; D015996:Survival Rate
D017127:Glycation End Products, Advanced