Alterations in the activity of lysosomal hydrolases in the synovial lining cell population of the knee joint of the rabbit during the development of chronic arthritis.
Arthritis has been induced in the knee joint of rabbits by the method of Dumonde and Glynn (1962); the animals were killed at various intervals up to 3 months. Unfixed cryostat sections of the synovial lining from control and arthritic joints have been used for the assay of lysosomal acid phosphatase, naphthylamidase, beta-glucuronidase and beta-N-acetylglucosaminidase. Enzyme activity in the synovial lining cell population has been quantitated by scanning and integrating microdensitometry. Beginning one week after induction of inflammation in the joint, significant elevations in the activity of all enzymes assayed were found in the synoviocytes of the inflamed joints.
Henderson
B
B
Division of Cellular Biology, Kennedy Institute of Rheumatology, Bute Gardens, London W6 7DW, U.K.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D001169:Arthritis, Experimental / Q000201:enzymology*; D002908:Chronic Disease; D005966:Glucuronidase / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism*; D007719:Knee Joint / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D011817:Rabbits; D013583:Synovial Membrane / Q000201:enzymology*
D006867:Hydrolases; D000135:Acid Phosphatase; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D000626:Aminopeptidases