Two methods for determining plasma IL-6 in humans at rest and following exercise.
We compared two methods to determine IL-6 at rest and following exercise. On two occasions, plasma IL-6 was determined in 10 men and 13 women before and immediately following 90 min of cycling at 65% V(O)(2max) using a cytometric bead array (CBA) and an ELISA. The CBA detected IL-6 in 63% of pre-exercise samples and 84% of post-exercise samples; the ELISA detected IL-6 in all samples. In samples with detectable IL-6, resting values were comparable between the CBA and ELISA (2.3 +/- 1.0 and 2.4 +/- 1.2 pg/mL, respectively; p = 0.99); the IL-6 response to exercise was underestimated (p = 0.0003) by the CBA by ~45%, compared with the ELISA. CBA- and ELISA-determined IL-6 values were moderately correlated (r = 0.66, p < 0.0001), but exhibited poor agreement (mean bias +/- 1SD = -1.4 +/- 2.1 pg/mL). The CBA provided concordant IL-6 concentrations at rest, when detected, but underestimated exercise-induced increases in IL-6 versus the ELISA method.
Timmons
Brian W
BW
Children's Exercise and Nutrition Centre, McMaster University, Chedoke Hospital, Evel Bldg, Sanatorium Road, Hamilton, ON, L8N 3Z5, Canada. timmonbw@mcmaster.ca
Hamadeh
Mazen J
MJ
Tarnopolsky
Mark A
MA
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015444:Exercise / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology; D010807:Physical Endurance; D012146:Rest / Q000502:physiology*
D015850:Interleukin-6
J Appl Physiol (1985). 1993 Oct;75(4):1897-902
8282648
Am J Physiol Regul Integr Comp Physiol. 2006 Aug;291(2):R285-90
16284083
J Leukoc Biol. 2005 Oct;78(4):819-35
16033812
J Physiol. 2000 Nov 15;529 Pt 1:237-42
11080265
J Appl Physiol Respir Environ Exerc Physiol. 1984 Mar;56(3):613-8
6706769
J Physiol. 1998 May 1;508 ( Pt 3):949-53
9518745
J Appl Physiol (1985). 2005 Apr;98(4):1154-62
15772055
J Appl Physiol (1985). 2005 Sep;99(3):979-85
15879167
Cytokine. 2005 Oct 7;32(1):45-50
16153856
Proc Nutr Soc. 2004 May;63(2):381-5
15294059
J Sports Sci. 2006 Jan;24(1):1-2
16368608
Trends Immunol. 2004 Jan;25(1):4-7
14698276
Exerc Immunol Rev. 2003;9:48-57
14686094
J Physiol. 2004 May 1;556(Pt 3):983-1000
14766942
Clin Immunol. 2004 Mar;110(3):252-66
15047203
Clin Chem. 2003 Jun;49(6 Pt 1):1000-2
12766012