title: Domain-specific regulation of recombination in Caenorhabditis elegans in response to temperature, age and sex.
abstract: It is generally considered that meiotic recombination rates increase with temperature, decrease with age, and differ between the sexes. We have reexamined the effects of these factors on meiotic recombination in the nematode Caenorhabditis elegans using physical markers that encompass >96% of chromosome III. The only difference in overall crossover frequency between oocytes and male sperm was observed at 16 degrees . In addition, crossover interference (CI) differs between the germ lines, with oocytes displaying higher CI than male sperm. Unexpectedly, our analyses reveal significant changes in crossover distribution in the hermaphrodite oocyte in response to temperature. This feature appears to be a general feature of C. elegans chromosomes as similar changes in response to temperature are seen for the X chromosome. We also find that the distribution of crossovers changes with age in both hermaphrodites and females. Our observations indicate that it is the oocytes from the youngest mothers-and not the oldest-that showed a different pattern of crossovers. Our data enhance the emerging hypothesis that recombination in C. elegans, as in humans, is regulated in large chromosomal domains.
authors.affiliation: Department of Biology, Goucher College, Baltimore, Maryland 21204, USA.
authors.forename: Jaclyn G Y
authors.initials: JG
authors.lastname: Lim
authors.forename: Rachel R W
authors.initials: RR
authors.lastname: Stine
authors.forename: Judith L
authors.initials: JL
authors.lastname: Yanowitz
mesh_terms: D000367:Age Factors; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D003434:Crossing Over, Genetic; D005260:Female; D017238:Genes, Helminth; D008297:Male; D008957:Models, Genetic; D009865:Oocytes / Q000378:metabolism; D011995:Recombination, Genetic; D012098:Reproduction / Q000235:genetics / Q000502:physiology; D013090:Spermatocytes / Q000378:metabolism; D013696:Temperature; D014960:X Chromosome
references.citation: Science. 2008 Mar 7;319(5868):1395-8
references.pmid: 18239090
references.citation: Genetics. 1998 Jan;148(1):167-85
references.pmid: 9475730
references.citation: Semin Cell Dev Biol. 2007 Oct;18(5):682-90
references.pmid: 17919949
references.citation: Cell. 2002 Dec 13;111(6):791-802
references.pmid: 12526806
references.citation: Curr Biol. 2007 Dec 4;17(23):2003-12
references.pmid: 18060788
references.citation: PLoS Genet. 2007 Feb 16;3(2):e25
references.pmid: 17305431
references.citation: Genetics. 1979 Jun;92(2):409-18
references.pmid: 17248928
references.citation: Genetics. 1974 May;77(1):71-94
references.pmid: 4366476
references.citation: Genetics. 1986 Nov;114(3):769-89
references.pmid: 3539697
references.citation: Mol Cell Biol. 2007 Mar;27(5):1868-80
references.pmid: 17189430
references.citation: Nat Genet. 2001 Jun;28(2):160-4
references.pmid: 11381264
references.citation: Science. 1923 Aug 17;58(1494):124-6
references.pmid: 17814166
references.citation: Plasmid. 2008 Jan;59(1):45-53
references.pmid: 18023874
references.citation: Proc Natl Acad Sci U S A. 1997 May 13;94(10):5213-8
references.pmid: 9144217
references.citation: Biotechniques. 2005 Dec;39(6):885-93
references.pmid: 16382908
references.citation: Cell. 2004 Mar 19;116(6):795-802
references.pmid: 15035982
references.citation: Proc Natl Acad Sci U S A. 1917 Sep;3(9):553-5
references.pmid: 16586748
references.citation: Mech Ageing Dev. 1977 Nov-Dec;6(6):413-29
references.pmid: 926867
references.citation: Genetics. 1979 Jan;91(1):67-94
references.pmid: 17248881
references.citation: Genetics. 1990 Oct;126(2):355-63
references.pmid: 2245915
references.citation: Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):87-9
references.pmid: 9419331
references.citation: Curr Biol. 2004 Sep 21;14(18):1639-43
references.pmid: 15380065
references.citation: Science. 2002 Jun 21;296(5576):2225-9
references.pmid: 12029063
references.citation: Cell Mol Life Sci. 2007 Jan;64(2):117-24
references.pmid: 17219025
references.citation: Int Rev Cytol. 2007;261:1-46
references.pmid: 17560279
references.citation: PLoS Biol. 2004 Sep;2(9):E259
references.pmid: 15309048
references.citation: J Cell Sci. 2004 Aug 15;117(Pt 18):4025-32
references.pmid: 15316078
references.citation: Development. 2006 Oct;133(19):3907-17
references.pmid: 16968818
references.citation: Genetics. 2001 Dec;159(4):1573-93
references.pmid: 11779798
references.citation: Dev Biol. 1976 Mar;49(1):200-19
references.pmid: 943344
references.citation: Funct Integr Genomics. 2000 Nov;1(3):200-6
references.pmid: 11793238
references.citation: Hum Mol Genet. 2000 Oct;9(16):2409-19
references.pmid: 11005796
references.citation: PLoS Genet. 2006 Feb;2(2):e12
references.pmid: 16462941
references.citation: Curr Biol. 2003 Sep 16;13(18):1641-7
references.pmid: 13678597
references.citation: Mol Cell. 2000 Sep;6(3):605-16
references.pmid: 11030340
references.citation: Genetics. 2002 Nov;162(3):1169-77
references.pmid: 12454064
references.citation: Genetics. 2004 Nov;168(3):1275-92
references.pmid: 15579685
references.citation: BMC Genomics. 2005 Sep 12;6:118
references.pmid: 16156901
references.citation: Nucleic Acids Res. 2008 Feb;36(3):984-97
references.pmid: 18096626
references.citation: Genetics. 1925 Sep;10(5):470-507
references.pmid: 17246286
references.citation: Genetics. 1995 Sep;141(1):159-79
references.pmid: 8536965
references.citation: PLoS Genet. 2008 Jul 11;4(7):e1000119
references.pmid: 18617997
references.citation: Mol Cell Biol. 1999 Jul;19(7):4832-42
references.pmid: 10373533
references.citation: Proc Natl Acad Sci U S A. 1926 Aug;12(8):530-2
references.pmid: 16587124
references.citation: Cell. 2004 Apr 2;117(1):29-45
references.pmid: 15066280
references.citation: Mutat Res. 1971 Jul;12(3):269-79
references.pmid: 5563942
references.citation: Genes Dev. 2008 Jan 15;22(2):194-211
references.pmid: 18198337
references.citation: Genetics. 2005 Nov;171(3):1047-56
references.pmid: 16118192
references.citation: Dev Biol. 2007 Aug 1;308(1):206-21
references.pmid: 17599823
references.citation: Cell. 2006 Apr 7;125(1):59-69
references.pmid: 16615890
references.citation: Nat Genet. 2001 Oct;29(2):229-32
references.pmid: 11586305
references.citation: Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11383-90
references.pmid: 11027339
references.citation: Nature. 2007 Oct 18;449(7164):851-61
references.pmid: 17943122
references.citation: Chromosoma. 1982;86(4):577-93
references.pmid: 7172867
references.citation: Nat Genet. 2007 Oct;39(10):1273-7
references.pmid: 17828265
references.citation: Development. 1998 Jul;125(13):2451-6
references.pmid: 9609828
references.citation: Curr Biol. 2004 Dec 29;14(24):R1036-7
references.pmid: 15620632
references.citation: Genetics. 1969 May;62(1):67-78
references.pmid: 5371018
references.citation: Trends Genet. 2007 Nov;23(11):539-42
references.pmid: 17964681
references.citation: Trends Genet. 2003 Sep;19(9):514-22
references.pmid: 12957545
references.citation: Trends Genet. 1995 Jan;11(1):27-31
references.pmid: 7900192
references.citation: Genetics. 2000 Oct;156(2):617-30
references.pmid: 11014811
references.citation: Nat Genet. 2005 Dec;37(12):1351-5
references.pmid: 16258540
references.citation: Nat Struct Mol Biol. 2007 Nov;14(11):1049-55
references.pmid: 17984967
references.citation: Nat Biotechnol. 1998 Nov;16(11):1054-9
references.pmid: 9831035
references.citation: PLoS Biol. 2005 Mar;3(3):e63
references.pmid: 15736976
references.citation: J Gerontol. 1994 Nov;49(6):B270-6
references.pmid: 7963273
references.citation: Development. 2002 Jan;129(2):479-92
references.pmid: 11807039