title: Flow cytometry and environmental microbiology.
abstract: This survey unit discusses many of the issues involved for flow cytometry in the field of microbiology, particularly the preparative procedures, which are far more stringent than many other procedures using larger cells. For instance, it is often necessary to filter laboratory agents multiple times to obtain the true particle-free solutions needed for flow cytometry of microbes. It is difficult enough to recognize bacteria in cell extracts from soil, sediment, or sludge given the background of same-size particles. This unit provides an excellent overview of a potentially large application area in flow cytometry and is written by one of the most respected scientists in the field.
authors.affiliation: University of Exeter, Exeter, United Kingdom.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Porter
mesh_terms: D001419:Bacteria / Q000166:cytology / Q000302:isolation & purification; D019411:Clinical Laboratory Techniques; D004783:Environmental Microbiology; D005434:Flow Cytometry / Q000379:methods*; D007202:Indicators and Reagents / Q000592:standards
chemical_list: D007202:Indicators and Reagents