Optimization of nutrient component for diesel oil degradation by Rhodococcus erythropolis.
A novel bacterium T7-2 was isolated from the oil-polluted sea-bed mud of Bohai Sea, northern China, which can degrade diesel oil at 15 degrees C. This bacterium was identified as a strain of Rhodococcus erythropolis according to its 16S rDNA gene. In order to enhance degradation efficiency, a five-level, three-factor central composite design was employed to optimize the nutrition supplied to artificial seawater. The results indicate that a supplement of 2.53 g (NH(4))(2)SO(4)L(-1), 2.75 g Na(2)HPO(4)L(-1) and 0.01 g yeast extract L(-1) to artificial seawater increases the degradation rate from 12.61% to 75% within 7d.
Huang
Lei
L
The School of Chemistry and Chemical Engineering, Tianjin University of Technology, 263 Hongqi South Road, Tianjin 300191, China.
Ma
Ting
T
Li
Dan
D
Liang
Feng-lai
FL
Liu
Ru-Lin
RL
Li
Guo-qiang
GQ
D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry; D005742:Gasoline; D019015:Geologic Sediments / Q000382:microbiology; D009792:Oceans and Seas; D012240:Rhodococcus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D012623:Seawater / Q000737:chemistry
D003470:Culture Media; D005742:Gasoline