title: Rapid differentiation of superficial urothelial cells after chitosan-induced desquamation.
abstract: Superficial cell desquamation followed by differentiation of newly exposed superficial cells induces regeneration of the urinary bladder epithelium, urothelium. In the present work, chitosan was evaluated as a new inducer of urothelial cell desquamation, in order to study the regeneration of mouse urothelial cells in vivo. Intravesical application of chitosan dispersion caused complete removal of only the superficial layer of cells within 20 min of treatment. Differentiation of the new superficial layer was followed by the appearance and distribution of three urothelial differentiation markers, tight junction protein ZO1, cytokeratin 20 and the maturation of the apical plasma membrane. The arrangement of ZO1 into continuous lines in individual cells of the intermediate layer was already found after 10 min of chitosan application, when desquamation had just started. The appearance of the apical membrane changed from microvillar to typically scalloped within 20 min of regeneration, while complete arrangement of the cytokeratin 20 network took 60 min. These findings provide a new perspective on the rate of the differentiation process in the urothelium and make chitosan a new and a very controllable tool for studies on urothelial regeneration.
authors.affiliation: Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Lipiceva 2, Ljubljana, Slovenia. peter.veranic@mf.uni-lj.si
authors.forename: Peter
authors.initials: P
authors.lastname: Veranic
authors.forename: Andreja
authors.initials: A
authors.lastname: Erman
authors.forename: Mojca
authors.initials: M
authors.lastname: Kerec-Kos
authors.forename: Marija
authors.initials: M
authors.lastname: Bogataj
authors.forename: Ales
authors.initials: A
authors.lastname: Mrhar
authors.forename: Kristijan
authors.initials: K
authors.lastname: Jezernik
mesh_terms: D000818:Animals; D016923:Cell Death; D002454:Cell Differentiation; D048271:Chitosan / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008855:Microscopy, Electron, Scanning; D019459:Urothelium / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D048271:Chitosan
references.citation: Cancer. 1969 May;23(5):1074-85
references.pmid: 5778231
references.citation: Exp Eye Res. 1993 Sep;57(3):283-92
references.pmid: 8224016
references.citation: Histochem Cell Biol. 1999 Nov;112(5):375-80
references.pmid: 10603077
references.citation: J Control Release. 2003 Apr 29;89(2):151-65
references.pmid: 12711440
references.citation: Invest Ophthalmol Vis Sci. 1993 Aug;34(9):2655-64
references.pmid: 8344789
references.citation: Invest Urol. 1970 Jul;8(1):116-23
references.pmid: 5433175
references.citation: Infect Immun. 2001 Jul;69(7):4572-9
references.pmid: 11402001
references.citation: J Nutr Biochem. 2002 Mar;13(3):157-167
references.pmid: 11893480
references.citation: Trends Biochem Sci. 2007 Jan;32(1):37-43
references.pmid: 17141506
references.citation: Pharm Res. 1997 Sep;14(9):1197-202
references.pmid: 9327448
references.citation: Histochem Cell Biol. 2001 Apr;115(4):309-16
references.pmid: 11405059
references.citation: Histochem Cell Biol. 2005 Jun;123(4-5):529-39
references.pmid: 15868181
references.citation: Am J Physiol Renal Physiol. 2004 Aug;287(2):F305-18
references.pmid: 15068973
references.citation: Science. 1992 Nov 6;258(5084):955-64
references.pmid: 1439807
references.citation: Proc Soc Exp Biol Med. 1988 Oct;189(1):13-20
references.pmid: 3186761
references.citation: Cancer Res. 1961 Dec;21:1577-89
references.pmid: 14486208
references.citation: Int J Pharm. 2004 Aug 20;281(1-2):11-23
references.pmid: 15288339
references.citation: Acta Anat (Basel). 1996;155(3):163-71
references.pmid: 8870784
references.citation: Pharm Res. 1994 Sep;11(9):1358-61
references.pmid: 7816770
references.citation: J Mol Biol. 1999 Jan 15;285(2):595-608
references.pmid: 9878432
references.citation: Eur J Cell Biol. 2004 Feb;83(1):27-34
references.pmid: 15085953
references.citation: Biol Pharm Bull. 2006 Aug;29(8):1685-91
references.pmid: 16880626
references.citation: Cell Biol Int. 2001;25(6):531-7
references.pmid: 11407858
references.citation: Virchows Arch B Cell Pathol. 1975;18(1):35-49
references.pmid: 807024
references.citation: Eur J Pharm Sci. 2005 May;25(1):113-21
references.pmid: 15854807
references.citation: Cell Tissue Res. 1992 May;268(2):239-45
references.pmid: 1617698
references.citation: Am J Physiol Renal Physiol. 2002 Dec;283(6):F1200-7
references.pmid: 12388410
references.citation: Biol Cell. 2000 Apr;92(2):105-13
references.pmid: 10879631
references.citation: J Urol. 1994 Aug;152(2 Pt 2):692-5; discussion 696-7
references.pmid: 8021997
references.citation: Am J Anat. 1960 May;106:247-85
references.pmid: 13769795
references.citation: Am J Pathol. 1995 Nov;147(5):1383-97
references.pmid: 7485401
references.citation: Int J Urol. 2006 Oct;13(10):1344-6
references.pmid: 17010016
references.citation: Cell Motil Cytoskeleton. 2002 Dec;53(4):317-25
references.pmid: 12378541
references.citation: Jpn J Cancer Res. 2001 Apr;92(4):459-66
references.pmid: 11346469
references.citation: Virchows Arch B Cell Pathol Incl Mol Pathol. 1981;38(1):117-25
references.pmid: 6119832
references.citation: Science. 1998 Nov 20;282(5393):1494-7
references.pmid: 9822381
references.citation: Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;57(1):27-36
references.pmid: 2567547
references.citation: Infect Immun. 1994 Dec;62(12):5505-10
references.pmid: 7960132
references.citation: Urol Res. 1982;10(5):227-37
references.pmid: 7164218
references.citation: Histochem Cell Biol. 1998 Mar;109(3):263-9
references.pmid: 9541475
references.citation: Infect Immun. 1988 Jun;56(6):1615-7
references.pmid: 3286502
references.citation: Mol Biol Cell. 2002 Mar;13(3):830-46
references.pmid: 11907265
references.citation: Br J Cancer Suppl. 1986;7:280-91
references.pmid: 3521706
references.citation: Biol Pharm Bull. 1995 Nov;18(11):1623-5
references.pmid: 8593495
references.citation: J Control Release. 2001 May 14;72(1-3):133-44
references.pmid: 11389992
references.citation: Lab Invest. 1967 Jan;16(1):44-65
references.pmid: 4164460