A stress-inducible resveratrol O-methyltransferase involved in the biosynthesis of pterostilbene in grapevine.
Stilbenes are considered the most important phytoalexin group in grapevine (Vitis vinifera) and they are known to contribute to the protection against various pathogens. The main stilbenes in grapevine are resveratrol and its derivatives and, among these, pterostilbene has recently attracted much attention due both to its antifungal and pharmacological properties. Indeed, pterostilbene is 5 to 10 times more fungitoxic than resveratrol in vitro and recent studies have shown that pterostilbene exhibits anticancer, hypolipidemic, and antidiabetic properties. A candidate gene approach was used to identify a grapevine resveratrol O-methyltransferase (ROMT) cDNA and the activity of the corresponding protein was characterized after expression in Escherichia coli. Transient coexpression of ROMT and grapevine stilbene synthase in tobacco (Nicotiana benthamiana) using the agroinfiltration technique resulted in the accumulation of pterostilbene in tobacco tissues. Taken together, these results showed that ROMT was able to catalyze the biosynthesis of pterostilbene from resveratrol both in vitro and in planta. ROMT gene expression in grapevine leaves was induced by different stresses, including downy mildew (Plasmopara viticola) infection, ultraviolet light, and AlCl(3) treatment.
Schmidlin
Laure
L
Laboratoire de Génétique et d'Amélioration de la Vigne, UMR1131, INRA, Université Louis Pasteur Strasbourg, F-68021 Colmar, France.
Poutaraud
Anne
A
Claudel
Patricia
P
Mestre
Pere
P
Prado
Emilce
E
Santos-Rosa
Maria
M
Wiedemann-Merdinoglu
Sabine
S
Karst
Francis
F
Merdinoglu
Didier
D
Hugueney
Philippe
P
D018076:DNA, Complementary; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D013312:Stress, Physiological; D027843:Vitis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000502:physiology
D018076:DNA, Complementary; D013267:Stilbenes; C107773:pterostilbene; D008780:Methyltransferases; D000077185:Resveratrol
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