Reactivity of thymine doublet in single strand DNA with osmium reagent.
Osmium oxidation has been frequently utilized for thymine-targeting DNA sequencing because of its high selectivity. In this study, we investigated the reactivity of various DNAs containing multiple thymine bases. A thymine doublet was more easily oxidized than a single thymine in single strand DNA.
Nomura
Akiko
A
Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Okamoto
Akimitsu
A
D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000737:chemistry*; D007202:Indicators and Reagents; D009942:Organometallic Compounds / Q000737:chemistry*; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry; D013941:Thymine / Q000737:chemistry*
D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; D009942:Organometallic Compounds; D009992:Osmium; D013941:Thymine; D013936:Thymidine