title: Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells.
abstract: We examined a novel human feeder cell layer of mesenchymal stem cells harvested from human adipose tissues. Gene expression analyses and colony-forming assay with human primary epithelial cells showed that the adipose tissue-derived mesenchymal stem cells produced various factors to support epithelial stem/progenitor maintenance and cell growth. Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces.
authors.affiliation: Department of Ophthalmology, Osaka University Medical School, Osaka 565-0871, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Maeda
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Hayashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Soma
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Hayashida
authors.forename: Joseph
authors.initials: J
authors.lastname: Yang
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shirakabe
authors.forename: Akifumi
authors.initials: A
authors.lastname: Matsuyama
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kikuchi
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tano
authors.forename: Kohji
authors.initials: K
authors.lastname: Nishida
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008858:Microscopy, Phase-Contrast / Q000379:methods; D008954:Models, Biological; D041681:NIH 3T3 Cells; D013234:Stem Cells; D023822:Tissue Engineering / Q000379:methods