[Cloning and phylogenetic analysis of bovine prochymosin gene].
The cDNA sequence of bovine prochymosin gene was cloned and sequenced from the abomasums of suckling calf by RT-PCR. The sequence was aligned and bioinformatically analyzed with related sequences in GenBank. The result of sequence analysis revealed that the gene was determined to bovine prochymosin B gene and had the high level of homology with prochymosin gene of other known mammals. The base bias of 18 species of prochymosin gene reduced according to codon position, and the gene provided us with excellent material of phylogenetic research. Thus, the phylogenetic tree of 18 species of prochymosin gene was used to discuss and offer testimony to phylogenetic relationship of 11 mammals.
Sun
Da-Qing
DQ
Key Lab of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China. sundaqing1979@163.com
Jiang
Yu-Jun
YJ
Han
Xi-Yan
XY
Qu
Yan-Yan
YY
Bi
Yu-Han
YH
Zhang
Guang-Hui
GH
D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D012085:Chymosin / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004792:Enzyme Precursors / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004792:Enzyme Precursors; C032443:prorennin; D012085:Chymosin