Assessment of phagocyte functions by flow cytometry.
Phagocytes neutrophils, monocytes, and macrophages are crucial in the defense against infection. Their functions include phagocytosis, intra- and extracellular digestion of targets, oxidative burst, and chemotaxis. This extensive, detailed unit outlines a four-part procedure for in-depth investigation of these four functions that reflect the attacking and processing of pathogenic microorganisms. Written by the original instigator of flow-based assays in this area, this unit defines an area that is as old as flow itself. Numerous support protocols provide preparative procedures for bacterial targets, opsonins, and antigen-coated beads.
Bassøe
Carl-Fredrik
CF
Haukeland University Hospital, Bergen, Norway.
D000818:Animals; D000941:Antigens / Q000276:immunology; D001419:Bacteria / Q000276:immunology; D002633:Chemotaxis; D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D006863:Hydrogen-Ion Concentration; D007962:Leukocytes / Q000166:cytology; D008863:Microspheres; D010586:Phagocytes / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis; D010588:Phagosomes / Q000382:microbiology; D011137:Polystyrenes; D016897:Respiratory Burst; D044967:Serum; D015054:Zymosan / Q000378:metabolism
D000941:Antigens; D011137:Polystyrenes; D015054:Zymosan; D016650:Fluorescein-5-isothiocyanate