NaCl improves siRNA delivery mediated by nanoparticles of hydroxyethylated cationic cholesterol with amido-linker.
We investigated the effect of amido- (NP-OH) and carbamate linkers (NP-HAPC) in nanoparticles composed of hydroxyethylated cationic cholesterol on siRNA transfection. The presence of NaCl in forming a NP-OH nanoplex increased the suppressive effect of gene expression by increasing the size of the nanoplex and changing the cellular uptake mechanism from membrane fusion and clathrin-mediated endocytosis to clathrin- and caveolae-mediated endocytosis.
Hattori
Yoshiyuki
Y
Institute of Medicinal Chemistry, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan. yhattori@hoshi.ac.jp
Hagiwara
Ayako
A
Ding
Wuxiao
W
Maitani
Yoshie
Y
D001692:Biological Transport; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000737:chemistry; D004705:Endocytosis / Q000187:drug effects; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D053758:Nanoparticles; D034741:RNA, Small Interfering / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
D034741:RNA, Small Interfering; D012965:Sodium Chloride; D002784:Cholesterol; D008156:Luciferases