title: Protein profiling of human endometrial tissues in the midsecretory and proliferative phases of the menstrual cycle.
abstract: OBJECTIVE
  To identify the proteins displaying differential expression in midsecretory phase endometrium as compared with proliferative phase endometrium.
  
  
  METHODS
  Prospective study with two groups of women in the midsecretory or proliferative phase.
  
  
  METHODS
  Clinical research outpatient department.
  
  
  METHODS
  Healthy, regularly cycling women of proven fertility.
  
  
  METHODS
  Collection of endometrial biopsy samples.
  
  
  METHODS
  Image analysis software was used to compare two-dimensional protein maps of midsecretory phase endometrial tissues (MSE) with maps of proliferative phase endometrial tissues (PROE) and midsecretory phase uterine fluids (MSU). Matrix-assisted laser desorption/ionization time of flight in tandem (MALDI-TOF-TOF) analysis was carried out to identify eight proteins that were differentially expressed between the two phases and also to identify the spots that shared similar coordinates in the two-dimensional maps of MSE and MSU.
  
  
  RESULTS
  Densitometry analysis and subsequent MALDI-TOF-TOF analysis revealed up-regulation of calreticulin, the beta chain of fibrinogen, adenylate kinase isoenzyme 5, and transferrin in the PROE and of annexin V, alpha1-antitrypsin, creatine kinase, and peroxidoxin 6 in MSE compared with the other phase. Superimposition of the two-dimensional maps of MSE on those of MSU revealed the presence of heat-shock protein 27, transferrin, and alpha1-antitrypsin precursor in both endometrial tissues and uterine secretions.
  
  
  CONCLUSIONS
  Differentially expressed proteins identified in the present study could be of relevance in endowing the endometrium with receptivity.
authors.affiliation: Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
authors.forename: Tanu
authors.initials: T
authors.lastname: Parmar
authors.affiliation: Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
authors.forename: Sushama
authors.initials: S
authors.lastname: Gadkar-Sable
authors.affiliation: Division of Clinical Research, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
authors.forename: Lalita
authors.initials: L
authors.lastname: Savardekar
authors.affiliation: Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
authors.forename: Rajendra
authors.initials: R
authors.lastname: Katkam
authors.affiliation: Proteomics Facility, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
authors.forename: Shalmali
authors.initials: S
authors.lastname: Dharma
authors.affiliation: Division of Clinical Research, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
authors.forename: Pervin
authors.initials: P
authors.lastname: Meherji
authors.affiliation: Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
authors.forename: Chander Parkash
authors.initials: CP
authors.lastname: Puri
authors.affiliation: Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India. Electronic address: sachdevag@nirrh.res.in.
authors.forename: Geetanjali
authors.initials: G
authors.lastname: Sachdeva
mesh_terms: D000263:Adenylate Kinase / Q000378:metabolism; D000328:Adult; D017304:Annexin A5 / Q000378:metabolism; D037282:Calreticulin / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D005498:Follicular Phase / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D008183:Luteal Phase / Q000378:metabolism*; D011446:Prospective Studies; D040901:Proteomics; D014168:Transferrin / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000378:metabolism
chemical_list: D017304:Annexin A5; C115499:BBeta fibrinogen; D037282:Calreticulin; D014168:Transferrin; D000515:alpha 1-Antitrypsin; D005340:Fibrinogen; D003402:Creatine Kinase; D000263:Adenylate Kinase; C118782:adenylate kinase 5