title: Approaches for studying angiogenesis-related signal transduction.
abstract: Understanding how extracellular growth factors activate intracellular pathways that promote angiogenesis is a broad area of research. In this chapter, we outline the systematic dissection of vascular endothelial growth factor (VEGF)-mediated activation of endothelial nitric oxide synthase and other downstream targets that are relevant to the angiogenic response. These approaches may also be applied to most other angiogenic-factor signaling cascades.
authors.affiliation: Vascular Biology and Therapeutics Program, Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: Behrad
authors.initials: B
authors.lastname: Derakhshan
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Harrison
authors.forename: Qing Robert
authors.initials: QR
authors.lastname: Miao
authors.forename: William C
authors.initials: WC
authors.lastname: Sessa
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D049449:Luminescence; D019331:NG-Nitroarginine Methyl Ester / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000503:physiopathology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D009573:Nitrites; D042461:Vascular Endothelial Growth Factor A; D052250:Nitric Oxide Synthase Type III; D019331:NG-Nitroarginine Methyl Ester