title: Synthesis of four colors fluorescently labelled 3'-O-blocked nucleotides with fluoride cleavable blocking group and linker for array based Sequencing-by-Synthesis applications.
abstract: Reversible terminators having a fluoride cleavable 3'-O-blocking group are presented. Each nucleotide triphosphate is labelled by a fluorescent dye cleavable by the same reagent. We present here their synthesis, cleavage experiments and polymerase incorporation tests for a possible use in a process of Sequencing-by-Synthesis.
authors.affiliation: Institut für Organische Chemie und Chemische Biologie, J. W. Goethe Universität Frankfurt am Main, Max-von-Laue Str. 7, D-60438 Frankfurt am Main, Germany.
authors.forename: Diana C
authors.initials: DC
authors.lastname: Knapp
authors.forename: Angelika
authors.initials: A
authors.lastname: Keller
authors.forename: Jennifer
authors.initials: J
authors.lastname: D'Onofrio
authors.forename: Arvydas
authors.initials: A
authors.lastname: Lubys
authors.forename: Saulius
authors.initials: S
authors.lastname: Serva
authors.forename: Ants
authors.initials: A
authors.lastname: Kurg
authors.forename: Maido
authors.initials: M
authors.lastname: Remm
authors.forename: Marek
authors.initials: M
authors.lastname: Kwiatkowski
authors.forename: Joachim W
authors.initials: JW
authors.lastname: Engels
mesh_terms: D003116:Color; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA
chemical_list: D005456:Fluorescent Dyes; D009711:Nucleotides; D004259:DNA-Directed DNA Polymerase; D005459:Fluorides