Key issues in F2-isoprostane analysis.
A large body of evidence indicates that measurement of F2-isoprostanes, specific prostaglandin F2-like compounds derived from the non-enzymatic peroxidation of arachidonic acid, is a reliable biomarker of oxidant stress in the human body. Since the discovery of F2-isoprostanes in the early 1990s, a variety of analytical approaches has been introduced for the quantification of these novel compounds. The aim of the present review is to shed light on the available gas chromatographic-mass spectrometric assays for the measurement of plasma or urinary F2-isoprostanes and to highlight a number of issues which need to be addressed in order to implement F2-isoprostane measurement as a gold-standard biomarker of oxidative stress in biological samples.
Nourooz-Zadeh
Jaffar
J
Department of Clinical and Nutritional Biochemistry, Urmia University of Medical Sciences, P.O. Box 1138, Urmia 57135, Iran. jnouroozzadeh@yahoo.co.uk
D015415:Biomarkers / Q000097:blood / Q000737:chemistry* / Q000652:urine; D028441:F2-Isoprostanes / Q000097:blood / Q000737:chemistry* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D018384:Oxidative Stress
D015415:Biomarkers; D028441:F2-Isoprostanes