title: Distribution of immunoglobulin G subclasses of anti-thyroid peroxidase antibody in sera from patients with Hashimoto's thyroiditis with different thyroid functional status.
abstract: The mechanism of disease progression in Hashimoto's thyroiditis (HT) is still unclear. Anti-thyroid peroxidase antibody (TPOAb), a diagnostic hallmark of HT, is principally of the immunoglobulin G (IgG) isotype, and it appears to be a response to thyroid injury. The aim of our study was to evaluate the distribution of IgG subclasses of TPOAb in sera from patients with HT with different thyroid functional status. Sera from 168 patients with newly diagnosed HT were collected and divided into three groups according to thyroid function: patients with hypothyroidism (n = 66), subclinical hypothyroidism (n = 60) and euthyroidism (n = 42). Antigen-specific enzyme-linked immunosorbent assay was used to detect the distribution of TPOAb IgG subclasses. The prevalence of TPOAb IgG subclasses in all patients' sera with HT was IgG1 70.2%, IgG2 35.1%, IgG3 19.6% and IgG4 66.1% respectively. The prevalence of IgG2 in sera from patients with hypothyroidism (51.5%) was significantly higher than that of subclinical hypothyroidism (33.3%) (P < 0.05), and the latter was also significantly higher than that of euthyroidism (11.9%) (P < 0.05). The positive percentage of IgG2 subclass in sera from patients with hypothyroidism and subclinical hypothyroidism was significantly higher than that of euthyroidism (P < 0.05), the prevalence and positive percentage of IgG4 subclass in sera from patients with hypothyroidism and subclinical hypothyroidism was significantly higher than that of euthyroidism respectively (P < 0.05). The predominant TPOAb IgG subclasses in sera from patients with HT were IgG1 and IgG4. Patients with high levels of TPOAb IgG2, IgG4 subclasses might be at high risk of developing overt hypothyroidism.
authors.affiliation: Department of Endocrinology, Peking University First Hospital, Beijing, China.
authors.forename: L-D
authors.initials: LD
authors.lastname: Xie
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: M-R
authors.initials: MR
authors.lastname: Li
authors.forename: G-Z
authors.initials: GZ
authors.lastname: Lu
authors.forename: X-H
authors.initials: XH
authors.lastname: Guo
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D050031:Hashimoto Disease / Q000150:complications / Q000276:immunology* / Q000503:physiopathology; D006801:Humans; D007037:Hypothyroidism / Q000209:etiology / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood*; D007453:Iodide Peroxidase / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013961:Thyroid Gland / Q000276:immunology / Q000503:physiopathology*; D013972:Thyrotropin / Q000097:blood
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D007074:Immunoglobulin G; D013972:Thyrotropin; D007453:Iodide Peroxidase
references.citation: Horm Res. 2003;59(3):118-24
references.pmid: 12637791
references.citation: Clin Endocrinol (Oxf). 1995 Jul;43(1):55-68
references.pmid: 7641412
references.citation: Eur J Endocrinol. 1995 Jan;132(1):69-74
references.pmid: 7850011
references.citation: Mol Immunol. 1985 Mar;22(3):161-206
references.pmid: 3889592
references.citation: Clin Endocrinol (Oxf). 1987 Mar;26(3):335-46
references.pmid: 3652478
references.citation: Scand J Immunol. 1989 Jul;30(1):73-82
references.pmid: 2756340
references.citation: J Clin Endocrinol Metab. 1997 Mar;82(3):925-31
references.pmid: 9062508
references.citation: N Engl J Med. 1996 Jul 11;335(2):99-107
references.pmid: 8649497
references.citation: J Exp Med. 1987 Nov 1;166(5):1351-61
references.pmid: 3500259
references.citation: J Immunol. 1994 Dec 1;153(11):4948-58
references.pmid: 7963558
references.citation: Nature. 1988 Apr 21;332(6166):738-40
references.pmid: 3258649
references.citation: J Clin Endocrinol Metab. 1996 Jul;81(7):2595-600
references.pmid: 8675583
references.citation: Neuroimmunomodulation. 2004;11(4):209-13
references.pmid: 15249726
references.citation: Ann Allergy. 1987 Feb;58(2):89-96, 99
references.pmid: 3544971
references.citation: J Clin Endocrinol Metab. 1993 Dec;77(6):1700-5
references.pmid: 7903315
references.citation: Clin Exp Immunol. 1986 Jan;63(1):73-9
references.pmid: 3485488
references.citation: Autoimmunity. 1989;4(4):247-54
references.pmid: 2491649
references.citation: J Clin Endocrinol Metab. 2002 Apr;87(4):1754-61
references.pmid: 11932312
references.citation: Annu Rev Immunol. 1989;7:407-44
references.pmid: 2469440
references.citation: Clin Exp Immunol. 1984 Jul;57(1):239-43
references.pmid: 6430614
references.citation: Mol Immunol. 1992 Mar;29(3):319-26
references.pmid: 1557042
references.citation: J Clin Endocrinol Metab. 1994 Aug;79(2):395-400
references.pmid: 8045954
references.citation: J Clin Endocrinol Metab. 1986 May;62(5):945-9
references.pmid: 2420816
references.citation: Eur J Endocrinol. 2003 Apr;148(4):383-8
references.pmid: 12656657