Interleukin-10 is an essential modulator of mucoid metaplasia in a mouse otitis media model.
OBJECTIVE
Inflammatory cytokines are involved in the development of mucous cell metaplasia and hyperplasia (MCM) in otitis media (OM). However, which cytokines play an essential role in the MCM of OM is not clear at the moment.


METHODS
In this study, we hypothesized that interleukin-10 (IL-10) played an indispensable role in the MCM of bacterial OM, and we used IL-10 knockout mice to test this hypothesis.


RESULTS
In wild-type mice, both Streptococcus pneumoniae and Haemophilus influenzae triggered the development of MCM in the middle ear mucosa. In IL-10 knockout mice, the number of goblet cells and mucin-producing cells in the middle ear was significantly reduced after bacterial middle ear infection compared with that in wild-type mice.


CONCLUSIONS
We conclude that IL-10 plays an essential role in the MCM of bacterial OM. Interleukin-10 is a potential target for the treatment of MCM in OM.
Tsuchiya
Katsuyuki
K
University of Minnesota Otitis Media Research Center, Department of Otolaryngology, University of Minnesota Medical School, Minneapolis, Minnesota, USA.
Komori
Masahiro
M
Zheng
Qing Yin
QY
Ferrieri
Patricia
P
Lin
Jizhen
J
D000818:Animals; D004195:Disease Models, Animal; D004428:Ear Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D004432:Ear, Middle / Q000187:drug effects* / Q000473:pathology; D020397:Goblet Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006192:Haemophilus Infections / Q000150:complications; D006193:Haemophilus influenzae; D016753:Interleukin-10 / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009077:Mucins / Q000378:metabolism; D009092:Mucous Membrane / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D010033:Otitis Media / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013290:Streptococcal Infections / Q000150:complications; D013296:Streptococcus pneumoniae
D009077:Mucins; D016753:Interleukin-10
J Assoc Res Otolaryngol. 2003 Sep;4(3):384-93
14690056
Am J Respir Crit Care Med. 1999 Dec;160(6):2040-7
10588626
Ann Otol Rhinol Laryngol. 2002 May;111(5 Pt 1):415-22
12018326
Infect Immun. 2000 Jul;68(7):4024-31
10858218
Laryngoscope. 2007 Jan;117(1):86-91
17135982
J Immunol. 2002 Jan 1;168(1):348-55
11751980
Am J Respir Cell Mol Biol. 1995 Sep;13(3):257-61
7544594
Circ Res. 2000 Sep 15;87(6):448-52
10988235
Nature. 1997 Oct 16;389(6652):737-42
9338786
Laryngoscope. 1991 Feb;101(2):165-9
1992267
J Immunol. 2002 Mar 1;168(5):2383-92
11859129
Curr Opin Microbiol. 2001 Jun;4(3):274-9
11378478
Ann Otol Rhinol Laryngol. 1998 Jun;107(6):501-7
9635460
Annu Rev Immunol. 1993;11:165-90
8386517
Int Immunol. 1995 Apr;7(4):517-23
7547677
J Immunol. 1993 Dec 15;151(12):6853-61
8258695
J Immunol. 2000 Aug 1;165(3):1612-7
10903771
Eur Arch Otorhinolaryngol. 2000;257(6):323-6
10993552
J Histochem Cytochem. 1991 Jan;39(1):71-80
1983875
J Infect Dis. 1999 May;179(5):1145-51
10191216
J Allergy Clin Immunol. 1998 Mar;101(3):293-7
9525442
J Biol Chem. 2002 Sep 20;277(38):35466-74
12107190
J Exp Med. 1991 Nov 1;174(5):1209-20
1940799
Acta Otolaryngol. 2002 Mar;122(2):153-60
11936906
Dig Dis Sci. 2002 Oct;47(10):2286-97
12395902
Adv Immunol. 1994;56:1-26
8073945
J Interferon Cytokine Res. 1995 May;15(5):441-5
7648446
Immunity. 1999 Oct;11(4):443-51
10549626
J Infect Dis. 2000 Sep;182(3):882-7
10950784
APMIS. 2003 Oct;111(10):989-94
14616553