[Inhibitory effects of transcription factor E2F-1 siRNA on invasion and proliferation of gastric cancer cell line MGC803].
OBJECTIVE
As an important regulatory factor of cell cycle, transcription factor E2F-1 is closely related to tumorigenesis. This study was to investigate the effects of E2F-1 small interfering RNA (siRNA) on invasion and proliferation of human gastric cancer MGC803 cells.


METHODS
E2F-1 siRNA vector containing short hairpin structure was transfected into MGC803 cells. Untransfected and pSilencer4.1-negative-transfected cells were used as controls. The expression of E2F-1 was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Cell matrigel invasion assay and cloning assay were used to detect the invasion and proliferation of MGC803 cells after E2F-1 siRNA transfection.


RESULTS
At 48 h after E2F-1 siRNA transfection, the mRNA level of E2F-1 was down-regulated by over 90.0% of controls; the protein level of E2F-1 was down-regulated by 79.6% of negative control and by 81.5% of empty control. The number of migrated cells was significantly smaller in E2F-1 siRNA group than in negative and empty control groups (18.0+/-2.6 vs. 48.0+/-4.6 and 54.0+/-5.6, P<0.05). The number of cell clones was also significantly smaller in E2F-1 siRNA group than in negative and empty control groups (46.0+/-2.0 vs. 122.3+/-1.5 and 128.7+/-2.1, P<0.05)


CONCLUSIONS
E2F-1 siRNA could down-regulate E2F-1 expression in human gastric cancer MGC803 cells and suppress its invasion and proliferation to some extent.
Yin
Yong-Shuo
YS
Department of Gastrointestinal and Gland Surgery, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, 530027, PR China .
Xiao
Qiang
Q
Xie
Yu-Bo
YB
Li
Lei
L
Wang
Chang-Qing
CQ
Ma
Yu-Lin
YL
Tang
Zheng-Yong
ZY
D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D050687:E2F1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness; D010957:Plasmids; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014162:Transfection
D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011994:Recombinant Proteins