Molecular determinants of matrix metalloproteinase-12 covalent modification by a photoaffinity probe: insights into activity-based probe development and conformational variability of matrix metalloproteinases.
Mass spectroscopy, microsequencing, and site-directed mutagenesis studies have been performed to identify in human matrix metalloelastase (hMMP-12) residues covalently modified by a photoaffinity probe, previously shown to be able to covalently label specifically the active site of matrix metalloproteinases (MMPs). Results obtained led us to conclude that photoactivation of this probe in complex with hMMP-12 affects a single residue in human MMP-12, Lys(241), through covalent modification of its side chain epsilon NH(2) group. Because x-ray and NMR studies of hMMP-12 indicate that Lys(241) side chain is highly flexible, our data reveal the existence of particular Lys(241) side-chain conformation in which the epsilon NH(2) group points toward the photolabile group of the probe, an event explaining the high levels of cross-linking yield between hMMP-12 and the probe. Lys(241) is not conserved in MMPs, thus differences in cross-linking yields observed with this probe between MMP members may be linked to the residue variability observed at position 241 in this family.
Dabert-Gay
Anne-Sophie
AS
Service d'Ingénierie Moléculaire de Protéines, CE-Saclay, Gif/Yvette 91191, Cedex, France.
Czarny
Bertrand
B
Devel
Laurent
L
Beau
Fabrice
F
Lajeunesse
Evelyne
E
Bregant
Sarah
S
Thai
Robert
R
Yiotakis
Athanasios
A
Dive
Vincent
V
D020134:Catalytic Domain / Q000502:physiology; D006801:Humans; D008239:Lysine / Q000737:chemistry* / Q000235:genetics; D013058:Mass Spectrometry; D053508:Matrix Metalloproteinase 12 / Q000737:chemistry* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D019920:Photoaffinity Labels / Q000737:chemistry*
D019920:Photoaffinity Labels; C507122:MMP12 protein, human; D053508:Matrix Metalloproteinase 12; D008239:Lysine
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