title: The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI.
abstract: The overproduction, purification, and in vitro characterization of the polyene glycosyltransferases (GTs) AmphDI and NysDI are reported. A novel nucleotidyltransferase mutant (RmlA Q83D) for the chemoenzymatic synthesis of unnatural GDP-sugar donors in conjunction with polyene GT-catalyzed sugar exchange/reverse reactions allowed the donor and acceptor specificities of these novel enzymes to be probed. The evaluation of polyene GT aglycon and GDP-sugar donor specificity revealed some tolerance to aglycon structural diversity, but stringent sugar specificity, and culminated in new polyene analogues in which L-gulose or D-mannose replace the native sugar D-mycosamine.
authors.affiliation: Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, UW-National Cooperative Drug Discovery Group, Program, University of Wisconsin-Madison, Madison, Wisconsin 53705-2222, USA.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Rocco
authors.initials: R
authors.lastname: Moretti
authors.forename: Jiqing
authors.initials: J
authors.lastname: Jiang
authors.forename: Jon S
authors.initials: JS
authors.lastname: Thorson
mesh_terms: D055162:Biocatalysis; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D011090:Polyenes / Q000737:chemistry* / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology
chemical_list: D006027:Glycosides; D011090:Polyenes; D006153:Guanosine Diphosphate; D016695:Glycosyltransferases
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