title: Analysis of cationic comb-type copolymers/DNA interaction by the single molecular observation and intermolecular force measurement.
abstract: We have reported that poly(L-lysine)-graft-dextran (PLL-g-Dex) accelerates DNA hybridization and increases stability of a double stranded DNA (dsDNA). Furthermore, PLL-g-Dex was found to stimulate the DNA strand exchange reaction between dsDNA and its complementary single stranded DNA (ssDNA). In order to better understand these phenomena, we evaluate PLL-g-Dex/DNA interaction by the single molecular observation and intermolecular force measurement at single molecular level. We have been able to observe PLL-g-Dex/DNA complex at single molecular level. PLL-g-Dex was found to increases the dissociation force of a dsDNA.
authors.affiliation: Institute for Materials Chemistry and Engineerng, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-8581, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Hirano
authors.forename: Naohiko
authors.initials: N
authors.lastname: Shimada
authors.forename: Arihiro
authors.initials: A
authors.lastname: Kano
authors.forename: Satoru
authors.initials: S
authors.lastname: Kidoaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Maruyama
mesh_terms: D004247:DNA / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D003911:Dextrans; C113090:poly(lysine)-graft-dextran; D011107:Polylysine; D004247:DNA