title: Functionalization of DNA G-Wires for patterning and nanofabrication.
abstract: DNA structures made of guanine tetrads present remarkable properties and are thus first choice candidates for applications in nanofabrication. Starting from the work of Kotlyar et al., we report here that the klenow exo(-) fragment of DNA polymerase I can extend poly(dG)-poly(dC) from various 5'-modified (dG)(10(-))(dC)(10) templates. This allows the production of end-functionalized four-stranded wires (G-Wires) assembled from the folding of poly(dG) strands. G-Wires bearing thiol moieties can be easily combed on Au and Pt surfaces, whereas a 5' single-stranded overhang of a random sequence provides the unique possibility to assemble complex structures for nanoconstruction purposes.
authors.affiliation: Muséum national d'Histoire naturelle USM 503, INSERM U565, CNRS UMR 5153, 43 rue Cuvier 75005 Paris, France. slyonnais@mnhn.fr
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lyonnais
authors.forename: Olivier
authors.initials: O
authors.lastname: Piétrement
authors.forename: Alexei
authors.initials: A
authors.lastname: Chepelianski
authors.forename: Sophie
authors.initials: S
authors.lastname: Guéron
authors.forename: Laurent
authors.initials: L
authors.lastname: Lacroix
authors.forename: Eric
authors.initials: E
authors.lastname: Le Cam
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mergny
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D004256:DNA Polymerase I / Q000378:metabolism; D054856:G-Quadruplexes; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D011068:Poly G / Q000737:chemistry; D011089:Polydeoxyribonucleotides / Q000096:biosynthesis
chemical_list: D011089:Polydeoxyribonucleotides; D011068:Poly G; C016681:poly(dG).poly(dC); C026628:poly(dG); D004247:DNA; D004256:DNA Polymerase I