Association of MMP-3 5A/6A gene polymorphism with susceptibility to carotid atherosclerosis.
OBJECTIVE
Stromelysin-1 (MMP-3) as a key member of metalloproteinase family could have an important role in atherogenesis. The 5A/6A polymorphism in the promoter of MMP-3 gene affects the level of MMP-3 gene expression. We assessed whether the MMP-3 promoter low- and high-activity genotypes are related to susceptibility for carotid atherosclerosis (CA) in Serbian population.


METHODS
The study group of case-control design consisted of 515 participants. The 265 patients with ultrasonographic evidence of carotid plaque presence were recruited for the study. The 5A/6A polymorphism was typed by RFLP-PCR.


RESULTS
There was significantly higher prevalence of genotypes containing 6A allele in the patients with CA compared to controls (p<0.05). The model of inheritance with the dominant effect of 6A allele gave elevated and significant OR for carotid atherosclerosis (adjusted OR 2.35, CI=1.0-5.5, p=0.048).


CONCLUSIONS
Subjects carrying genotypes with 6A allele had significantly higher susceptibility to carotid atherosclerosis.
Djurić
Tamara
T
VINCA Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, P.O. Box 522, 11001 Belgrade, Serbia. tamariska@vin.bg.ac.yu
Zivković
Maja
M
Radak
Djordje
D
Jekić
Djole
D
Radak
Sandra
S
Stojković
Ljiljana
L
Raicević
Ranko
R
Stanković
Aleksandra
A
Alavantić
Dragan
D
D000328:Adult; D002340:Carotid Artery Diseases / Q000201:enzymology* / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3