title: Utilization of arylaliphatic nitriles by haloalkaliphilic Halomonas nitrilicus sp. nov. isolated from soda soils.
abstract: An enrichment culture from saline soda soils, using acetate as carbon and energy source and 2-phenylpropionitrile as nitrogen source (PPN) at pH 10, resulted in the isolation of strain ANL-alpha CH3. The strain was identified as a representative of the genus Halomonas in the Gammaproteobacteria. The bacterium was capable of PPN utilization as a nitrogen source only, while phenylacetonitrile (PAN) served both as carbon, energy and nitrogen source. This capacity was not described previously for any other haloalkaliphilic bacteria. Apart from the nitriles mentioned above, resting cells of ANL-alpha CH3 also hydrolyzed mandelonitrile, benzonitrile, acrylonitrile, and phenylglycinonitrile, presumably using nitrilase pathway. Neither nitrile hydratase nor amidase activity was detected. The isolate showed a capacity to grow with benzoate and salicylate as carbon and energy source and demonstrated the ability to completely mineralize PAN. These clearly indicated a potential to catabolize aromatic compounds. On the basis of unique phenotype and distinct phylogeny, strain ANL-alpha CH3 is proposed as a novel species of the genus Halomonas--Halomonas nitrilicus sp. nov.
authors.affiliation: Biocatalysis & Organic Chemistry, Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Chmura
authors.forename: A A
authors.initials: AA
authors.lastname: Shapovalova
authors.forename: S
authors.initials: S
authors.lastname: van Pelt
authors.forename: F
authors.initials: F
authors.lastname: van Rantwijk
authors.forename: T P
authors.initials: TP
authors.lastname: Tourova
authors.forename: G
authors.initials: G
authors.lastname: Muyzer
authors.forename: D Yu
authors.initials: DY
authors.lastname: Sorokin
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000097:Acetonitriles / Q000378:metabolism*; D000181:Acrylonitrile / Q000378:metabolism; D000619:Aminohydrolases / Q000378:metabolism; D019817:Benzoic Acid / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009570:Nitriles / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012459:Salicylates / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D000097:Acetonitriles; D009570:Nitriles; D012336:RNA, Ribosomal, 16S; D012459:Salicylates; C083412:2-phenylpropionitrile; C006725:benzyl cyanide; C045516:mandelonitrile; D002244:Carbon; D019817:Benzoic Acid; C014356:benzonitrile; D000619:Aminohydrolases; C021867:nitrilase; D006836:Hydro-Lyases; C048831:nitrile hydratase; D000181:Acrylonitrile; D009584:Nitrogen; D019342:Acetic Acid
references.citation: Antonie Van Leeuwenhoek. 1998 Jul-Oct;74(1-3):89-97
references.pmid: 10068792
references.citation: Arch Microbiol. 2008 Jul;190(1):89-100
references.pmid: 18437357
references.citation: Appl Microbiol Biotechnol. 2002 Oct;60(1-2):33-44
references.pmid: 12382040
references.citation: Comput Appl Biosci. 1994 Sep;10(5):569-70
references.pmid: 7828077
references.citation: Environ Sci Technol. 2003 Oct 1;37(19):4397-402
references.pmid: 14572091
references.citation: Microbiology (Reading). 2007 Apr;153(Pt 4):1157-1164
references.pmid: 17379725
references.citation: Biochem J. 1979 Aug 15;182(2):609-11
references.pmid: 508302
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713
references.citation: Curr Opin Chem Biol. 2000 Feb;4(1):95-102
references.pmid: 10679370
references.citation: J Mol Biol. 1962 Jul;5:109-18
references.pmid: 14470099
references.citation: Water Res. 2005 Feb;39(4):648-54
references.pmid: 15707638
references.citation: Appl Environ Microbiol. 2007 Sep;73(17):5574-9
references.pmid: 17644641
references.citation: Toxicon. 2000 Jan;38(1):11-36
references.pmid: 10669009
references.citation: Nat Biotechnol. 1998 Aug;16(8):733-6
references.pmid: 9702770
references.citation: Angew Chem Int Ed Engl. 2004 Feb 6;43(7):788-824
references.pmid: 14767950