title: Laser-induced fluorescence imaging of subsurface tissue structures with a volume holographic spatial-spectral imaging system.
abstract: A three-dimensional imaging system incorporating multiplexed holographic gratings to visualize fluorescence tissue structures is presented. Holographic gratings formed in volume recording materials such as a phenanthrenquinone poly(methyl methacrylate) photopolymer have narrowband angular and spectral transmittance filtering properties that enable obtaining spatial-spectral information within an object. We demonstrate this imaging system's ability to obtain multiple depth-resolved fluorescence images simultaneously.
authors.affiliation: Department of Electrical and Computing Engineering, University of Arizona, Tucson, AZ 85721, USA. luo@email.arizona.edu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Luo
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Gelsinger-Austin
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Watson
authors.forename: George
authors.initials: G
authors.lastname: Barbastathis
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Barton
authors.forename: Raymond K
authors.initials: RK
authors.lastname: Kostuk
mesh_terms: D000165:Acridine Orange; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D003106:Colon / Q000166:cytology; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods; D005456:Fluorescent Dyes; D006696:Holography / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D051379:Mice
chemical_list: D005456:Fluorescent Dyes; D000165:Acridine Orange