[Arg-Gly-Asp-containing peptide combining with the biomimetic and modified PLGA-(ASP-PEG)].
Arg-Gly-Asp-(RGD) containing peptide characterized as the non-viral gene vector was synthesized to modify the surface of PLGA-(ASP-PEG). The Peptide (K16-GRGDSPC) was synthesized. PLGA-(ASP-PEG) was executed into chips A, B and C. Chip C was regarded as control. Chips A and B reacted with the cross-linker, then Chip A reacted with peptide. Mass spectrometry (MS) and high performance liquid chromatography (HPLC) detected the molecular weight and the purity of peptide. Sulphur in the surface of materials was detected by X-ray photoelectron spectrometry (XPS). The peptide content in the residual solution was detected by Spectrometer. HPLC showed the peptide purity was 94.13%; MS showed the molecular weight was 2741.26. XPS revealed the binding energy of the sulphur in reacted Chip A was 164 eV in reacted Chip B, 164eV and 162 eV; the ratios of carbon to sulphur in reacted Chip A and B were 99.746:0.1014 and 99.574:0.4255, respectively. There was no sulphur in Chip C. The optical density value (OD) of the resident solution was 0.069. The peptide density of reacted Chip A was 0.04 mg/mm2. The peptide was manufactured and linked to the surface of the biomimetic PLGA-(ASP-PEG) with the cross-linker.
Song
Yulin
Y
Department of Orthopaedics, Union Hospital, Tongji Medical College of Huazhong Science and Technology University, Wan 430022, China.
Zheng
Qixin
Q
Guo
Xiaodong
X
Hao
Jie
J
D001224:Aspartic Acid / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D005822:Genetic Vectors / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013499:Surface Properties; D023822:Tissue Engineering
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D009842:Oligopeptides; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D001224:Aspartic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid