Protein profiling of human endometrial tissues in the midsecretory and proliferative phases of the menstrual cycle.
OBJECTIVE
To identify the proteins displaying differential expression in midsecretory phase endometrium as compared with proliferative phase endometrium.


METHODS
Prospective study with two groups of women in the midsecretory or proliferative phase.


METHODS
Clinical research outpatient department.


METHODS
Healthy, regularly cycling women of proven fertility.


METHODS
Collection of endometrial biopsy samples.


METHODS
Image analysis software was used to compare two-dimensional protein maps of midsecretory phase endometrial tissues (MSE) with maps of proliferative phase endometrial tissues (PROE) and midsecretory phase uterine fluids (MSU). Matrix-assisted laser desorption/ionization time of flight in tandem (MALDI-TOF-TOF) analysis was carried out to identify eight proteins that were differentially expressed between the two phases and also to identify the spots that shared similar coordinates in the two-dimensional maps of MSE and MSU.


RESULTS
Densitometry analysis and subsequent MALDI-TOF-TOF analysis revealed up-regulation of calreticulin, the beta chain of fibrinogen, adenylate kinase isoenzyme 5, and transferrin in the PROE and of annexin V, alpha1-antitrypsin, creatine kinase, and peroxidoxin 6 in MSE compared with the other phase. Superimposition of the two-dimensional maps of MSE on those of MSU revealed the presence of heat-shock protein 27, transferrin, and alpha1-antitrypsin precursor in both endometrial tissues and uterine secretions.


CONCLUSIONS
Differentially expressed proteins identified in the present study could be of relevance in endowing the endometrium with receptivity.
Parmar
Tanu
T
Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
Gadkar-Sable
Sushama
S
Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
Savardekar
Lalita
L
Division of Clinical Research, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
Katkam
Rajendra
R
Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
Dharma
Shalmali
S
Proteomics Facility, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
Meherji
Pervin
P
Division of Clinical Research, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
Puri
Chander Parkash
CP
Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
Sachdeva
Geetanjali
G
Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India. Electronic address: sachdevag@nirrh.res.in.
D000263:Adenylate Kinase / Q000378:metabolism; D000328:Adult; D017304:Annexin A5 / Q000378:metabolism; D037282:Calreticulin / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D005498:Follicular Phase / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D008183:Luteal Phase / Q000378:metabolism*; D011446:Prospective Studies; D040901:Proteomics; D014168:Transferrin / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000378:metabolism
D017304:Annexin A5; C115499:BBeta fibrinogen; D037282:Calreticulin; D014168:Transferrin; D000515:alpha 1-Antitrypsin; D005340:Fibrinogen; D003402:Creatine Kinase; D000263:Adenylate Kinase; C118782:adenylate kinase 5