A novel prophenoloxidase 2 exists in shrimp hemocytes.
The prophenoloxidase (proPO)-activating system in crustaceans and other arthropods is regarded as a constituent of the immune system and plays an important role in defense against pathogens. Hitherto in crustaceans, only one proPO gene per species has been identified. Here we report the identification of a novel proPO-2 (LvproPO-2) from the hemocytes of Litopenaeus vannamei, which shows 72% identity to proPO-1 (LvproPO-1) cloned previously. Northern blotting analysis and quantitative real-time PCR reveal that LvproPO-2 is mainly expressed in the hemocytes, and its expression is down-regulated in shrimp challenged with white spot syndrome virus (WSSV). Western blotting analysis shows that most LvproPO-2/LvPO-2 (L. vannamei phenoloxidase-2) exists in the hemocytes, but not in plasma of L. vannamei. LvproPO-2/LvPO-2 could be detected on the hemocyte surface and the nucleus of hemocytes by indirect immunofluorescence assay (IFA). These findings provide insight into the molecular biological basis for further studying on the defense mechanism of shrimp innate immunity, especially on the proPO-activating system and melanization cascade of shrimp.
Ai
Hua-Shui
HS
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen (Zhongshan) University, 135 Xingang Road West, Guangzhou 510275, PR China.
Liao
Ji-Xiang
JX
Huang
Xian-De
XD
Yin
Zhi-Xin
ZX
Weng
Shao-Ping
SP
Zhao
Zhi-Ying
ZY
Li
Se-Dong
SD
Yu
Xiao-Qiang
XQ
He
Jian-Guo
JG
D000595:Amino Acid Sequence; D000818:Animals; D004156:Catechol Oxidase / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism*; D006434:Hemocytes / Q000378:metabolism* / Q000821:virology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D010802:Phylogeny; D046848:White spot syndrome virus 1 / Q000502:physiology
D004792:Enzyme Precursors; C038451:pro-phenoloxidase; D004156:Catechol Oxidase