A perivascular origin for mesenchymal stem cells in multiple human organs.
Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-Rbeta expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.
Crisan
Mihaela
M
Department of Pediatrics, Stem Cell Research Center, Children's Hospital of Pittsburgh, Pittsburgh, PA 15213, USA.
Yap
Solomon
S
Casteilla
Louis
L
Chen
Chien-Wen
CW
Corselli
Mirko
M
Park
Tea Soon
TS
Andriolo
Gabriella
G
Sun
Bin
B
Zheng
Bo
B
Zhang
Li
L
Norotte
Cyrille
C
Teng
Pang-Ning
PN
Traas
Jeremy
J
Schugar
Rebecca
R
Deasy
Bridget M
BM
Badylak
Stephen
S
Buhring
Hans-Jörg
HJ
Giacobino
Jean-Paul
JP
Lazzari
Lorenza
L
Huard
Johnny
J
Péault
Bruno
B
D000293:Adolescent; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology*; D000368:Aged; D002465:Cell Movement; D053686:Fetal Stem Cells / Q000166:cytology*; D005333:Fetus; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D024510:Muscle Development; D020286:Pericytes / Q000166:cytology*