title: Optimized fluoride ions treatment for release of base-sensitive 2'-O-modified oligoribonucleotides from various solid supports.
abstract: To synthesize oligoribonucleotides containing base-sensitive 2'-O-modifications, we used the Q-linker or a silyl linker anchored to CPG or polystyrene resin. A fluoride ions treatment was optimized to release oligoribonucleotides from the solid-support and to remove TBDMS groups without affecting 2'-O-acyloxymethyl groups.
authors.affiliation: IBMM, UMR 5247 CNRS-UM1-UM2, CC 1704, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lavergne
authors.forename: Françoise
authors.initials: F
authors.lastname: Debart
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Vasseur
mesh_terms: D000577:Amides / Q000737:chemistry; D005459:Fluorides / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: C522070:5-taurinomethyluridine; D000577:Amides; D007477:Ions; D009843:Oligoribonucleotides; D010756:Phosphoric Acids; C011067:phosphoramidic acid; D005459:Fluorides; D014529:Uridine