title: Intracellular mRNA imaging with a hybridization sensitive fluorescent nucleotide.
abstract: We reported a technique for detecting intracellular RNA with a newly developed functional probe. To detect mRNAs, a polyA tail targeting probe was synthesized. This probe showed quite large enhancement in the presence of its complementary RNA. In living cultured cells, the probe also showed strong fluorescence. This technique was effective for intracellular mRNA detection.
authors.affiliation: Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kubota
authors.forename: Shuji
authors.initials: S
authors.lastname: Ikeda
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D015345:Oligonucleotide Probes / Q000737:chemistry*; D011806:Quinolinium Compounds / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis*; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011806:Quinolinium Compounds; D012333:RNA, Messenger; D014529:Uridine