Analysis of low molecular weight GTPase activity in endothelial cell cultures.
GTPases control a myriad of cellular processes, including cell migration, proliferation, polarity, and cell adhesion, which includes cell-cell junction regulation. Angiogenesis requires many of these diverse cellular events to occur appropriately. In this chapter, we describe the techniques for in vitro assessment of GTPase activity in endothelial cells grown in monolayer culture as a model system for studying their role during angiogenesis.
Wittchen
Erika S
ES
Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Burridge
Keith
K
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D018919:Neovascularization, Physiologic / Q000502:physiology; D020764:cdc42 GTP-Binding Protein / Q000737:chemistry / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D020827:rap1 GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism
D020558:GTP Phosphohydrolases; D020764:cdc42 GTP-Binding Protein; D020744:rac GTP-Binding Proteins; D020827:rap1 GTP-Binding Proteins