Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans.
Under carbon starvation, Aspergillus nidulans produced a fungal/bacterial type chitinase, ChiB. The chiB gene was cloned and subcloned into pJC40 expression vector containing a 10XHis fusion tag, and the ChiB protein was expressed heterologously in Escherichia coli. Recombinant and native ChiB enzymes shared the same optimal pH ranges and showed similar substrate specificities with endo-acting cleavage patterns.
Erdei
Eva
E
Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology, University of Debrecen, H-4010 Debrecen, Hungary.
Pusztahelyi
Tünde
T
Miskei
M
M
Barna
Teréz
T
Pócsi
I
I
D001233:Aspergillus nidulans / Q000201:enzymology*; D015153:Blotting, Western; D002688:Chitinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D011994:Recombinant Proteins; D002688:Chitinases