[The study of apoptosis induction effect of vitamin E succinate on Tca8113 human tongue cancer cells].
OBJECTIVE
To investigate the apoptosis induction effect of vitamin E succinate (VES) on Tca8113 cells and its possible mechanisms.


METHODS
The proliferative activity of Tca8113 was assessed by methyl thiazolyl tetrazolium (MTT) assay. After Tca8113 cells were treated with different concentrations of VES, apoptotic rates were analyzed by flow cytometry (FCM). Fas monoclonal antibody was used for the blocking test. Fas expression was detected by immuocytochemistry(SABC assay) and FCM.


RESULTS
VES demonstrated a significant growth inhibitory effect and apoptosis induced effect on the Tca8113 cells in a dose- and time-dependent manner. Fas neutralizing antibody can block the apoptosis induced by VES. After the administration of VES, the expression of Fas protein increased and the kytoplasm staining enhanced. Proteinum quantitative analysis showed that the mean fluorescence intensity increased.


CONCLUSIONS
VES can induce apoptosis in human tongue cancer cells, and the up-regulation of the cell surface Fas protein may play an important role in the process.
Cao
Xuan-ping
XP
Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Zhengzhou University, Zhengzhou 450052, China.
Wang
Shu-bin
SB
Zhou
Hong
H
Wu
Hao-yang
HY
Zhang
Yan-xi
YX
Liu
Xue-jun
XJ
Zhang
Song-tao
ST
D017209:Apoptosis; D006801:Humans; D013386:Succinates; D014062:Tongue Neoplasms; D014810:Vitamin E
D013386:Succinates; D014810:Vitamin E