Cloning, genomic organization and functionality of 5-HT(7) receptor splice variants from mouse brain.
The serotonin (5-HT) 5-HT(7) receptors are expressed in both the central nervous system and in peripheral tissues. Receptor distribution studies and pharmacological studies have established that 5-HT(7) receptors play an important role in the control of circadian rhythms and thermoregulation. Selective 5-HT(7) receptor ligands have potential therapeutic applications for the treatment of pain and migraine, schizophrenia, anxiety, cognitive disturbances and inflammation. We have cloned two novel C-terminal splice variants of the 5-HT(7) receptor from mouse brain. These two new splice variants have almost identical sequences as the rat 5-HT(7(b)) and 5-HT(7(c)) splice variants and so were given the same name. Ligand binding assays ([(3)H]5-CT), membrane localization and functional studies in transiently transfected cells indicated that all three splice variants are well expressed on the membrane and no major differences in their respective pharmacology and their ability to activate adenylyl cyclase were observed. This is in analogy with previous reports comparing either the rat or the human variants.
Gellynck
Evelien
E
Laboratory for Eukaryotic Gene Expression and Signal Transduction (LEGEST), Ghent University, K.L. Ledeganckstraat 35, 9000 Gent, Belgium.
Laenen
Koen
K
Andressen
Kjetil Wessel
KW
Lintermans
Béatrice
B
De Martelaere
Kim
K
Matthys
Anne
A
Levy
Finn Olav
FO
Haegeman
Guy
G
Vanhoenacker
Peter
P
Van Craenenbroeck
Kathleen
K
D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism; D017398:Alternative Splicing; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D016466:CHO Cells; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D007668:Kidney / Q000166:cytology; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011869:Radioligand Assay; D051381:Rats / Q000235:genetics*; D011985:Receptors, Serotonin / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D011985:Receptors, Serotonin; C104425:serotonin 7 receptor; D000262:Adenylyl Cyclases