Mismatch recognition in PNA double-duplex invasion.
Double-duplex invasion of pseudo-complementary PNA (pcPNA) to double-stranded DNA is promising for recognition of a specific sequence in double-stranded DNA. In order to apply this process for various purposes such as gene suppression, one base-pair change at the target site in DNA must be strictly distinguished by the pcPNA additives. In this study, mismatch-recognizing activity of double-duplex invasion was investigated under various salt conditions. It has been found that the mismatch-recognition of the invasion is strict enough to distinguish one base-pair alternation, as long as the invasion is achieved in the media of appropriate ionic strength (e.g., [NaCl] = 20 mM).
Miyajima
Yoshitaka
Y
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 komaba, Meguro-ku, Tokyo, 153-8904, Japan.
Ishizuka
Takumi
T
Komiyama
Makoto
M
D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D024202:Electrophoretic Mobility Shift Assay; D009994:Osmolar Concentration; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D020135:Peptide Nucleic Acids; D004247:DNA; D004492:Edetic Acid