Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis.
Ser acetyltransferase (SERAT), which catalyzes O-acetyl-Ser (OAS) formation, plays a key role in sulfur assimilation and Cys synthesis. Despite several studies on SERATs from various plant species, the in vivo function of multiple SERAT genes in plant cells remains unaddressed. Comparative genomics studies with the five genes of the SERAT gene family in Arabidopsis thaliana indicated that all three Arabidopsis SERAT subfamilies are conserved across five plant species with available genome sequences. Single and multiple knockout mutants of all Arabidopsis SERAT gene family members were analyzed. All five quadruple mutants with a single gene survived, with three mutants showing dwarfism. However, the quintuple mutant lacking all SERAT genes was embryo-lethal. Thus, all five isoforms show functional redundancy in vivo. The developmental and compartment-specific roles of each SERAT isoform were also demonstrated. Mitochondrial SERAT2;2 plays a predominant role in cellular OAS formation, while plastidic SERAT2;1 contributes less to OAS formation and subsequent Cys synthesis. Three cytosolic isoforms, SERAT1;1, SERAT3;1, and SERAT3;2, may play a major role during seed development. Thus, the evolutionally conserved SERAT gene family is essential in cellular processes, and the substrates and products of SERAT must be exchangeable between the cytosol and organelles.
Watanabe
Mutsumi
M
Graduate School of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263-8522, Japan.
Mochida
Keiichi
K
Kato
Tomohiko
T
Tabata
Satoshi
S
Yoshimoto
Naoko
N
Noji
Masaaki
M
Saito
Kazuki
K
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D019143:Evolution, Molecular; D023281:Genomics / Q000379:methods*; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012694:Serine / Q000031:analogs & derivatives / Q000378:metabolism; D051050:Serine O-Acetyltransferase / Q000145:classification / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D020033:Protein Isoforms; D012694:Serine; D051050:Serine O-Acetyltransferase; C043943:O-acetylserine
Plant J. 2000 May;22(4):335-43
10849350
Eur J Biochem. 1998 Jul 1;255(1):235-45
9692924
Physiol Plant. 2008 Feb;132(2):190-8
18251860
Curr Opin Plant Biol. 2000 Jun;3(3):188-95
10837270
Plant Mol Biol. 2000 Dec;44(6):759-75
11202438
Plant Physiol. 2008 Jan;146(1):310-20
18024555
Science. 2000 Dec 15;290(5499):2114-7
11118139
Plant J. 1999 Oct;20(2):237-43
10571883
Plant J. 2005 Jan;41(1):15-30
15610346
Plant Physiol. 2000 Jul;123(3):1163-71
10889265
Mitochondrion. 2002 Nov;2(1-2):71-86
16120310
J Exp Bot. 2004 Aug;55(404):1785-98
15258168
Amino Acids. 2002;22(3):231-43
12083067
J Plant Physiol. 2006 Feb;163(3):273-86
16386330
Bioinformatics. 2006 Jul 1;22(13):1658-9
16731699
Methods Enzymol. 1985;113:548-55
4088074
Dev Biol. 1989 Jan;131(1):161-7
2909401
Plant Cell. 2002 Oct;14(10):2339-51
12368490
Nature. 2000 Dec 14;408(6814):796-815
11130711
Plant Physiol. 1996 Oct;112(2):659-667
12226419
Eur J Biochem. 1995 Jan 15;227(1-2):500-9
7851429
J Exp Bot. 2000 Jun;51(347):985-93
10948226
J Biol Chem. 1966 Nov 10;241(21):4955-65
5332668
Plant Physiol. 2005 Jan;137(1):220-30
15579666
Plant Physiol. 2004 Sep;136(1):2443-50
15375200
J Biol Chem. 1999 Jan 1;274(1):397-402
9867856
Plant Physiol. 1990 Nov;94(3):1345-52
16667839
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536-40
16593964
Methods Enzymol. 1987;143:85-96
3657565
Brief Bioinform. 2004 Jun;5(2):150-63
15260895
J Exp Bot. 2002 Aug;53(375):1689-98
12147719
Plant Physiol. 1994 Nov;106(3):887-95
7824657
Amino Acids. 2003;24(1-2):195-203
12624753
Amino Acids. 2002;22(3):245-57
12083068
Science. 2003 Aug 1;301(5633):653-7
12893945
Plant Cell. 2008 Jan;20(1):168-85
18223034
Plant Physiol. 2006 Jan;140(1):59-66
16339799
J Exp Bot. 2002 May;53(372):1283-304
11997376
Plant Physiol. 2006 Jun;141(2):446-55
16531482
Plant Cell. 2000 Jul;12(7):1093-101
10899976
Plant Physiol. 1996 Sep;112(1):273-80
8819326
Plant Physiol. 1999 Jul;120(3):637-44
10398698
Amino Acids. 2001;20(3):243-59
11354602
Amino Acids. 2006 Mar;30(2):173-83
16552493
Plant J. 2002 Dec;32(6):879-89
12492831
Annu Rev Plant Physiol Plant Mol Biol. 2000 Jun;51:141-165
15012189
Curr Opin Chem Biol. 2001 Oct;5(5):541-9
11578927
Eur J Biochem. 2000 Sep;267(18):5657-64
10971575
Plant J. 2008 Mar;53(6):999-1012
18088327
BMC Evol Biol. 2008 Feb 04;8:39
18248682
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5833-7
3475704
J Biol Chem. 1998 Dec 4;273(49):32739-45
9830017