title: Cancer/testis genes expression in human melanoma cell lines.
abstract: We analyzed the expression of 15 cancer/testis and four melanoma differentiation antigens in 21 metastatic melanoma cell lines using reverse transcriptase-polymerase chain reaction (RT-PCR) assay. On the basis of morphological characteristics, tumor cell lines were divided into three groups with high, moderate, and low grade of differentiation. Evaluation of gene expression and melanoma cell morphology has revealed a correlation between increased expression of cancer/testis genes and differentiation grade of cancer cells. The gene expression pattern for lymph node metastases and primary tumors exhibits the distribution of expression level and frequency similar to that found for established cell lines. Nevertheless, only 60% lymph node metastases or primary tumor tissue of randomly selected patients show marked expression of the most prominent cancer/testis genes, and almost 90% lesion tissue expresses at least one of 15 cancer/testis genes.
authors.affiliation: Russian N.N. Blokhin Memorial Cancer Research Center bNational Cardiology Research Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: Irina N
authors.initials: IN
authors.lastname: Mikhaylova
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Kovalevsky
authors.forename: Lidia F
authors.initials: LF
authors.lastname: Morozova
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Golubeva
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Cheremushkin
authors.forename: Marina I
authors.initials: MI
authors.lastname: Lukashina
authors.forename: Ekaterina S
authors.initials: ES
authors.lastname: Voronina
authors.forename: Olga S
authors.initials: OS
authors.lastname: Burova
authors.forename: Igor A
authors.initials: IA
authors.lastname: Utyashev
authors.forename: Sergei L
authors.initials: SL
authors.lastname: Kiselev
authors.forename: Lev V
authors.initials: LV
authors.lastname: Demidov
authors.forename: Robert Sh
authors.initials: RSh
authors.lastname: Beabealashvilli
authors.forename: Anatoly Y
authors.initials: AY
authors.lastname: Baryshnikov
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology / Q000556:secondary; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013737:Testis
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm