[Study on the distribution of sub-genotype B on hepatitis B virus in patients with chronic HBV infection from 4 cities of China].
OBJECTIVE
To investigate the distribution of sub-genotype B on hepatitis B virus (HBV) in patients with HBV chronic infection from 4 cities (Beijing, Shijiazhuang, Wenzhou and Shenzhen) of China.


METHODS
A type-specific nested polymerase chain reaction(PCR) with multiplex pairs of primers was used for HBV genotyping,and Ba and Bj sub-genotypes were identified by a PCR-restriction fragment length polymorphism (PCR-RFLP) method. A total of 101 serum samples collected from patients with chronic HBV/B infection were detected. Among them, 18 were collected from Beijing, 22 from Shijiazhuang, 34 from Wenzhou and 27 from Shenzhen. Thirty from the 101 serum samples were randomly selected and analyzed by PCR product sequencing.


RESULTS
All of the 101 serum samples were identified as sub-genotype Ba,and none of them was sub-genotype Bj.


CONCLUSIONS
Sub-genotype Ba was a predominant strain of HBV/B in patients with chronic HBV infection from these 4 cities.
Wang
Jie
J
Department of Microbiology, Peking University Health Science Center, Beijing 100083, China. jieli69@263.net
Gao
Jun-wei
JW
Zhuang
Hui
H
Li
Jie
J
Wang
Jia
J
Li
Ya-juan
YJ
Jin
Hui
H
D002681:China; D005838:Genotype; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics