Selective enzymatic labeling to detect packing-induced denaturation of double-stranded DNA at interfaces.
The adsorption of DNA on surfaces is a widespread procedure and is a common way for fabrication of biosensors, DNA chips, and nanoelectronic devices. Although the biologically relevant and prevailing in vivo structure of DNA is its double-stranded (dsDNA) conformation, the characterization of DNA on surfaces has mainly focused on single-stranded DNA (ssDNA). Studying the structure of dsDNA on surfaces is of invaluable importance to microarray performance since their effectiveness relies on the ability of two DNA molecules to hybridize and remain stable. In addition, many of the enzymatic transactions performed on DNA require dsDNA, rather than ssDNA, as a substrate. However, it is not established that adsorbed dsDNA remains in its structure and does not denature. Here, two methodologies have been developed for distinguishing between surface-adsorbed single- and double-stranded DNA. We demonstrate that, upon formation of a dense monolayer, the nonthiolated strand comprising the dsDNA is released and the monolayer consists of mostly ssDNA. The fraction of dsDNA within the ssDNA monolayer depends on the length of the oligomers. A likely mechanism leading to this rearrangement is discussed.
Peled
Dana
D
Department of Chemical Physics and Chemical Research Support, Weizmann Institute, Rehovot 76100, Israel.
Daube
Shirley S
SS
Naaman
Ron
R
D000327:Adsorption; D001483:Base Sequence; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D004256:DNA Polymerase I / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004581:Electronics; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D036103:Nanotechnology / Q000379:methods; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D011119:Polynucleotides / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
D004277:DNA, Single-Stranded; D011119:Polynucleotides; D004247:DNA; D004256:DNA Polymerase I; D012825:Silicon