[Biological effect of gamma-rays and LPS on the murine macrophage cell line RAW264.7 in mice].
OBJECTIVE
To explore the effect of activating the murine macrophage cell line RAW264.7 by both gamma-rays and lipopolysaccharide (LPS) and to study the expression of calcium-binding protein S100A8 induced by gamma-rays and LPS.


METHODS
The RAW264.7 cells were observed by phase contrast microscope. The cell cycle and the level of reactive oxygen intermediates (ROIs) were detected by flow cytometry (FCM). The production of NO was measured by colorimetric Griess reaction. The mRNA expression of S100A8 was recorded by real-time quantitative RT-PCR method.


RESULTS
The exposure of RAW264.7 cells to gamma-rays and LPS resulted in the morphological change of cells, the rise of cells number of aneuploid and apoptosis, and the rise of the level of ROI, NO and S100A8 mRNA. The effect of using both gamma-rays and LPS was stronger than that of single gamma-rays or LPS treatment.


CONCLUSIONS
The mechanism of using both gamma-rays and LPS for activating macrophages is owing to the various biological effects including the change of cell cycle, the change of the level of messenger molecules and the expression of inflammation factor such as S100A8. The expression of S100A8 gene is closely correlated with the function and state of macrophages.
Rao
Ya-lan
YL
Institute of Radiation Medicine, Academy of Military Medicine Sciences, Beijing 100850, China.
Cong
Yue
Y
Chen
Xiao-hua
XH
Dong
Bo
B
Li
Feng-sheng
FS
Zhang
Jun-quan
JQ
Gao
Ling
L
Mao
Bing-zhi
BZ
D000818:Animals; D040501:Calgranulin A; D002460:Cell Line; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects; D008070:Lipopolysaccharides / Q000276:immunology*; D008262:Macrophage Activation / Q000528:radiation effects; D008264:Macrophages / Q000276:immunology* / Q000528:radiation effects*; D051379:Mice; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism
D040501:Calgranulin A; D008070:Lipopolysaccharides; D009418:S100 Proteins; C086145:S100a8 protein, mouse