title: Probe labeling and fluorescence in situ hybridization.
abstract: This unit describes in detail basic protocols for probe labeling, denaturing of in situ target DNA, in situ hybridization, and post-hybridization washes. Support protocols for probe labeling cover probe purification and quality control.
authors.affiliation: Leiden University, Leiden, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Wiegant
authors.forename: A K
authors.initials: AK
authors.lastname: Raap
mesh_terms: D003582:Cytogenetics; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006801:Humans; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D016133:Polymerase Chain Reaction
chemical_list: D005456:Fluorescent Dyes; D004247:DNA