Anandamide effects on 5-HT(3) receptors in vivo.
Studies in knockout mouse strains have shown that some cannabimimetic effects persist in animals lacking cannabinoid CB(1) and CB(2) receptors. These residual effects are thought to result, in part, from a cannabinoid-modulation of ion channels. This study investigates the role of 5-HT(3) receptors as a potential in vivo target for cannabinoids. Mice deficient in CB(1) and CB(2) receptors were treated with Delta(9)-tetrahydrocannabinol and anandamide, in the presence of the 5-HT(3) antagonist ondansetron. We show that the cannabinoid receptor-independent anandamide analgesia, but not catalepsy, is completely blocked by ondansetron. Thus, 5-HT(3) receptors seem to be involved in cannabinoid analgesia.
Rácz
Ildikó
I
Institute of Molecular Psychiatry, Life & Brain Center, University of Bonn, Sigmund-Freud-Strasse 25, Bonn, Germany.
Bilkei-Gorzo
Andras
A
Markert
Astrid
A
Stamer
Friederike
F
Göthert
Manfred
M
Zimmer
Andreas
A
D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D013759:Dronabinol / Q000494:pharmacology; D063388:Endocannabinoids; D046352:Freezing Reaction, Cataleptic / Q000187:drug effects; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009043:Motor Activity / Q000187:drug effects; D017294:Ondansetron / Q000494:pharmacology; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D043884:Receptor, Cannabinoid, CB1 / Q000235:genetics*; D043885:Receptor, Cannabinoid, CB2 / Q000235:genetics*; D044406:Receptors, Serotonin, 5-HT3 / Q000502:physiology*; D058831:Serotonin 5-HT3 Receptor Antagonists
D000701:Analgesics, Opioid; D001095:Arachidonic Acids; D063388:Endocannabinoids; D053284:Polyunsaturated Alkamides; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D044406:Receptors, Serotonin, 5-HT3; D058831:Serotonin 5-HT3 Receptor Antagonists; D017294:Ondansetron; D013759:Dronabinol; C078814:anandamide