title: Synthesis and immunological evaluation of self-adjuvanting glycolipopeptide vaccine candidates.
abstract: Synthesis of four glycolipids with different number of lauroyl groups on glucose or cellobiose as scaffolds is described. Their immunological evaluations either admixed with or covalently linked to J8, a peptide antigen derived from the C-terminus of the antiphagocytic M-protein of group A streptococcus, are also investigated. Administration of mixtures of J8 and glycolipids to B10BR (H-2(k)) mice induced low-levels of J8-specific IgG antibodies. While glycolipopeptides, in which J8 was covalently linked to the synthetic glycolipids, demonstrated high-levels of antibody titers comparable with the co-administration of these glycolipopeptides with complete Freund's adjuvant, suggesting clearly the strong potency of the synthesized glycolipids as self-adjuvanting moieties.
authors.affiliation: School of Molecular and Microbial Sciences (SMMS), The University of Queensland, St. Lucia 4072, Qld, Australia.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Fujita
authors.forename: Abu-Baker M
authors.initials: AB
authors.lastname: Abdel-Aal
authors.forename: Norbert
authors.initials: N
authors.lastname: Wimmer
authors.forename: Michael R
authors.initials: MR
authors.lastname: Batzloff
authors.forename: Michael F
authors.initials: MF
authors.lastname: Good
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000378:metabolism; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology / Q000494:pharmacology; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage* / Q000276:immunology / Q000494:pharmacology; D006017:Glycolipids / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000494:pharmacology; D006020:Glycopeptides / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000494:pharmacology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010587:Phagocytosis; D013291:Streptococcus / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D006017:Glycolipids; D006020:Glycopeptides; D007074:Immunoglobulin G; C014689:streptococcal M protein; D005620:Freund's Adjuvant