Tyramide signal amplification (TSA) systems for the enhancement of ISH signals in cytogenetics.
This unit provides a series of techniques that dramatically improve the sensitivity of direct and indirect in situ detection. TSA is a peroxidase-based signal amplification system which is compatible with all in situ hybridization as well as immunocytochemical detection systems. The assay is performed on a glass slide and combines the use of fluorescent probes in either direct or indirect format using either an enzyme-labeled streptavidin or antibody. Protocols are provided for brightfield and fluorescent detection of single DNA targets, DNA or RNA in cultured cells, multitarget detection for DNA/RNA and DNA and RNA. In addition a protocol is included for the TSA plus system, a DNP-based detection system using horseradish peroxidase and alkaline phosphatase.
Bobrow
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MN
NEN Life Science Products, Boston, Massachusetts, USA.
Moen
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PT
D000818:Animals; D002384:Catalysis; D003582:Cytogenetics; D004247:DNA / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods*; D010544:Peroxidases / Q000737:chemistry; D012313:RNA / Q000032:analysis; D019809:Streptavidin / Q000737:chemistry; D014439:Tyramine / Q000032:analysis*
D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA; D019809:Streptavidin; D010544:Peroxidases; D014439:Tyramine