Impairment of germline transmission after blastocyst injection with murine embryonic stem cells cultured with mouse hepatitis virus and mouse minute virus.
The aim of this study was to determine the susceptibility of murine embryonic stem (mESCs) to mouse hepatitis virus (MHV-A59) and mouse minute virus (MMVp) and the effect of these viruses on germline transmission (GLT) and the serological status of recipients and pups. When recipients received 10 blastocysts, each injected with 10(0) TCID(50) MHV-A59, three out of five recipients and four out of 14 pups from three litters became seropositive. When blastocysts were injected with 10(-5) TCID(50) MMVp, all four recipients and 14 pups from four litters remained seronegative. The mESCs replicated MHV-A59 but not MMVp, MHV-A59 being cytolytic for mESCs. Exposure of mESCs to the viruses over four to five passages but not for 6 h affected GLT. Recipients were seropositive for MHV-A59 but not for MMVp when mESCs were cultured with the virus over four or five passages. The data show that GLT is affected by virus-contaminated mESCs.
Mahabir
E
E
Department of Comparative Medicine, Helmholtz Center Munich, Germany. mahabir@helmholtz-muenchen.de
Reindl
K
K
Mysliwietz
J
J
Needham
J
J
Bulian
D
D
Markoullis
K
K
Scherb
H
H
Schmidt
J
J
D000818:Animals; D001755:Blastocyst / Q000821:virology*; D018352:Coronavirus Infections / Q000821:virology*; D004624:Embryo Transfer; D053595:Embryonic Stem Cells / Q000821:virology*; D005260:Female; D005307:Fertilization in Vitro; D005434:Flow Cytometry; D005854:Germ Cells / Q000502:physiology*; D006524:Hepatitis, Viral, Animal / Q000276:immunology / Q000635:transmission*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008915:Minute Virus of Mice / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*; D006517:Murine hepatitis virus / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*; D010322:Parvoviridae Infections / Q000821:virology*; D011991:Receptors, Virus / Q000378:metabolism; D016036:Seroepidemiologic Studies; D014779:Virus Replication
D011991:Receptors, Virus
Comp Med. 2000 Aug;50(4):410-1
11020160
Biol Reprod. 2007 Feb;76(2):189-97
17021342
Hum Reprod. 2002 Mar;17(3):760-4
11870132
J Virol. 2002 Apr;76(8):3697-708
11907209
Lab Anim. 2003 Oct;37(4):341-51
14609003
J Gene Med. 2004 Feb;6 Suppl 1:S193-202
14978762
J Virol. 1972 Oct;10(4):586-90
4673484
Lab Anim Sci. 1977 Apr;27(2):173-9
192942
J Cell Physiol. 1977 Jun;91(3):393-401
558990
Int J Cancer. 1979 Mar 15;23(3):402-9
437922
J Virol. 1979 Aug;31(2):537-45
113559
J Gen Virol. 1981 Oct;56(Pt 2):457-60
6171615
J Reprod Fertil. 1982 Sep;66(1):161-8
7120180
J Virol. 1983 Jun;46(3):937-43
6602221
J Virol. 1983 Jun;46(3):944-55
6602222
J Leukoc Biol. 1986 May;39(5):559-65
3009672
Proc Natl Acad Sci U S A. 1986 Dec;83(23):9065-9
3024164
Vet Res Commun. 1988;12(2-3):199-217
2847406
J Infect Dis. 1993 Jun;167(6):1288-95
8388900
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8424-8
8378314
Lab Anim. 1994 Jan;28(1):1-12
8158962
Lab Anim. 1994 Jan;28(1):31-4
8158966
J Virol. 1996 Jun;70(6):4146-9
8648758
Exp Anim. 1997 Jan;46(1):11-6
9027466
Exp Anim. 1997 Oct;46(4):311-3
9353641
Lab Anim. 2002 Jan;36(1):20-42
11831737