title: Microsphere surface protein determination using flow cytometry.
abstract: This unit describes an extrinsic staining protocol using the amine-reactive CBQCA dye to measure the amount of protein on the surface of a microsphere. This approach is novel in that it allows microspheres bearing proteins without known binding partners to be accurately quantified on a flow cytometer.
authors.affiliation: Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
authors.forename: Travis A
authors.initials: TA
authors.lastname: Woods
authors.forename: Steven W
authors.initials: SW
authors.lastname: Graves
authors.forename: John P
authors.initials: JP
authors.lastname: Nolan
mesh_terms: D001565:Benzoates; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D008565:Membrane Proteins / Q000737:chemistry*; D008863:Microspheres; D011804:Quinolines; D013499:Surface Properties
chemical_list: D001565:Benzoates; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D011804:Quinolines; C069617:3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde