title: [Study on the interaction between vincristine and bovine serum albumin].
abstract: The interaction between vincristine (VCR) and bovine serum albumin (BSA) was investigated by UV-Vis absorption, fluorescence and circular dichroism (CD) spectra at 296, 303 and 310 K, respectively. With fluorescence quenching method, the binding constants Ka were determined to be 1.5 x 10(4) L x mol(-1), 9.5 x 10(3) L x mol(-1), 4.9 x 10(3) L x mol(-1) and the number of binding site was 1 at three temperatures, respectively. The conformation of BSA was altered (CD data) with the reductions of alpha-helices from 33.5% for free BSA to 29.7%, and with increases of beta-sheet from 13.6% for free BSA to 18.4% in the presence of VCR. The thermodynamic parameters, enthalpy change (deltaH) and entropy change (deltaS), were calculated to be -62.07 kJ x mol(-1) and -129.38 J x (mol x K)(-1) respectively, according to van't Hoff equation, which indicated that hydrogen bonds and van der walls interactions played major roles in the binding process.
authors.affiliation: College of Bioengineering, Dalian University, Dalian 116622, China. ckh20055@163.com
authors.forename: Ke-hai
authors.initials: KH
authors.lastname: Chen
authors.forename: Yu-lian
authors.initials: YL
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Guo
authors.forename: Xue-fang
authors.initials: XF
authors.lastname: Zheng
authors.forename: Qian
authors.initials: Q
authors.lastname: Tang
authors.forename: Jun-yan
authors.initials: JY
authors.lastname: Ma
authors.forename: Da-bin
authors.initials: DB
authors.lastname: Gao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D002942:Circular Dichroism; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014750:Vincristine / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012710:Serum Albumin, Bovine; D014750:Vincristine