The role of Mac-1 (CD11b/CD18) in osteoclast differentiation induced by receptor activator of nuclear factor-kappaB ligand.
Multinuclear osteoclasts are derived from CD11b-positive mononuclear cells in bone marrow and in circulation. FACS sorting experiments showed impaired osteoclastogenesis in RAW264.7 cells with low CD11b expression. Neutralizing antibodies and siRNA against CD11b inhibited osteoclastogenesis induced by RANKL. Although primary cultured mouse bone marrow macrophages expressed CD11a and CD11b, osteoclastogenesis induced by M-CSF and RANKL was inhibited in the presence of anti-CD11b or anti-CD18 but not anti-CD11a antibodies. Furthermore, anti-CD11b antibodies inhibited NFATc1 expression induced by M-CSF and RANKL in BMMs. These findings suggest, at least partly, an important role of CD11b in osteoclastogenesis.
Hayashi
Hidetaka
H
Department of Cellular Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Tokyo 113-8549, Japan.
Nakahama
Ken-ichi
K
Sato
Takahiro
T
Tuchiya
Takehiko
T
Asakawa
Yasuyuki
Y
Maemura
Toshimitu
T
Tanaka
Masanobu
M
Morita
Mineto
M
Morita
Ikuo
I
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology; D039481:CD11b Antigen / Q000235:genetics / Q000502:physiology; D018821:CD18 Antigens / Q000235:genetics / Q000502:physiology; D002452:Cell Count; D002454:Cell Differentiation; D002460:Cell Line; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology / Q000502:physiology; D016177:Macrophage-1 Antigen / Q000235:genetics / Q000502:physiology*; D008264:Macrophages / Q000166:cytology; D008297:Male; D051379:Mice; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D053245:RANK Ligand / Q000494:pharmacology / Q000502:physiology; D012342:RNA, Small Nuclear / Q000235:genetics
D000906:Antibodies; D039481:CD11b Antigen; D018821:CD18 Antigens; D016177:Macrophage-1 Antigen; D053245:RANK Ligand; D012342:RNA, Small Nuclear; D016173:Macrophage Colony-Stimulating Factor