title: Megakaryocytic expression of miRNA 10a, 17-5p, 20a and 126 in Philadelphia chromosome-negative myeloproliferative neoplasm.
abstract: Micro RNA (miRNA) are small non-coding RNA molecules which have a post-transcriptional inhibitory regulation function, e.g. in megakaryopoiesis. A characteristic of Philadelphia chromosome-negative myeloproliferative neoplasm (Ph(-) MPN) is the abundance of morphologically aberrant megakaryocytes. Based on previously published in vitro megakaryocytic differentiation assay data, we selected miRNA 10a, 17-5p, 20a and 126 and potential target proteins (HOXA1, RUNX1) for analysis of laser-microdissected bone marrow megakaryocytes from Ph(-) MPN and controls (n=66). Furthermore, we tested a potential influence of cytoreductive treatment on miRNA expression in bone marrow cells during the course of Ph(-) MPN (n=18). In summary, miRNA 17-5p, 20a and 126 are constitutively expressed in Ph(-) MPN megakaryopoiesis while low or absent miRNA 10a appeared to correlate with strong megakaryocytic HOXA1 protein expression. No association to thrombocytosis, JAK2(V617F) mutations or cytoreductive treatment (bone marrow cells) were observed.
authors.affiliation: Institute of Pathology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30615 Hanover, Germany. Hussein.Kais@MH-Hannover.de
authors.forename: Kais
authors.initials: K
authors.lastname: Hussein
authors.forename: Wiebke
authors.initials: W
authors.lastname: Dralle
authors.forename: Katharina
authors.initials: K
authors.lastname: Theophile
authors.forename: Hans
authors.initials: H
authors.lastname: Kreipe
authors.forename: Oliver
authors.initials: O
authors.lastname: Bock
mesh_terms: D001854:Bone Marrow Cells; D050676:Core Binding Factor Alpha 2 Subunit; D015870:Gene Expression; D018398:Homeodomain Proteins; D006801:Humans; D054438:Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative; D008533:Megakaryocytes / Q000378:metabolism*; D035683:MicroRNAs / Q000032:analysis*; D009196:Myeloproliferative Disorders / Q000235:genetics* / Q000473:pathology; D014157:Transcription Factors; D000099261:Homeobox A1 Protein
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D018398:Homeodomain Proteins; D035683:MicroRNAs; D014157:Transcription Factors; D000099261:Homeobox A1 Protein; C493728:RUNX1 protein, human