title: Expression of ammonia transporters, Rhbg and Rhcg, in chronic cyclosporine nephropathy in rats.
abstract: OBJECTIVE
  Cyclosporine (CsA)-induced renal injury causes renal tubular acidosis. The current study was performed to evaluate the influence of CsA-induced renal injury on the ammonia transporter family members, Rh B-glycoprotein (Rhbg) and Rh C-glycoprotein (Rhcg).
  
  
  METHODS
  Rats were treated daily for 1 or 4 weeks with vehicle (VH) or CsA. Induction of chronic CsA-induced nephropathy was confirmed by demonstrating impaired renal function and characteristic histopathology. Rhbg and Rhcg expression was evaluated with immunoblot, immunohistochemistry, real-time RT-PCR and electron microscopy.
  
  
  RESULTS
  CsA treatment for 4 weeks developed mild metabolic acidosis and decreased urinary ammonia excretion. Rhcg mRNA expression was unchanged in both the cortex and outer medulla, but Rhcg protein expression in the CsA group was significantly reduced in the cortex and outer medulla. There were no significant differences in Rhbg mRNA and protein expression between the CsA and VH group.
  
  
  CONCLUSIONS
  Long-term treatment with CsA in rats results in decreased urinary ammonia excretion accompanied by decreased expression of Rhcg; these changes are likely to mediate the CsA-induced defect in ammonium excretion in the collecting duct.
authors.affiliation: Department of Internal Medicine, Catholic University of Korea, Seoul, Korea.
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Lim
authors.forename: Kyung Ohk
authors.initials: KO
authors.lastname: Ahn
authors.forename: Wan Young
authors.initials: WY
authors.lastname: Kim
authors.forename: Dong He
authors.initials: DH
authors.lastname: Han
authors.forename: Can
authors.initials: C
authors.lastname: Li
authors.forename: Jung Yeon
authors.initials: JY
authors.lastname: Ghee
authors.forename: Ki Hwan
authors.initials: KH
authors.lastname: Han
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Mary E
authors.initials: ME
authors.lastname: Handlogten
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.forename: Chul Woo
authors.initials: CW
authors.lastname: Yang
authors.forename: I David
authors.initials: ID
authors.lastname: Weiner
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins / Q000096:biosynthesis* / Q000187:drug effects*; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology*; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D027682:Cation Transport Proteins; D008562:Membrane Glycoproteins; C508142:Rh type B glycoprotein, rat; C508143:Rh type C glycoprotein, rat; D016572:Cyclosporine
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