title: Cross-species bacterial artificial chromosome-fluorescence in situ hybridization painting of the tomato and potato chromosome 6 reveals undescribed chromosomal rearrangements.
abstract: Ongoing genomics projects of tomato (Solanum lycopersicum) and potato (S. tuberosum) are providing unique tools for comparative mapping studies in Solanaceae. At the chromosomal level, bacterial artificial chromosomes (BACs) can be positioned on pachytene complements by fluorescence in situ hybridization (FISH) on homeologous chromosomes of related species. Here we present results of such a cross-species multicolor cytogenetic mapping of tomato BACs on potato chromosomes 6 and vice versa. The experiments were performed under low hybridization stringency, while blocking with Cot-100 was essential in suppressing excessive hybridization of repeat signals in both within-species FISH and cross-species FISH of tomato BACs. In the short arm we detected a large paracentric inversion that covers the whole euchromatin part with breakpoints close to the telomeric heterochromatin and at the border of the short arm pericentromere. The long arm BACs revealed no deviation in the colinearity between tomato and potato. Further comparison between tomato cultivars Cherry VFNT and Heinz 1706 revealed colinearity of the tested tomato BACs, whereas one of the six potato clones (RH98-856-18) showed minor putative rearrangements within the inversion. Our results present cross-species multicolor BAC-FISH as a unique tool for comparative genetic studies across Solanum species.
authors.affiliation: Wageningen-UR Plant Breeding, PB Wageningen, The Netherlands.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Tang
authors.forename: Dóra
authors.initials: D
authors.lastname: Szinay
authors.forename: Chunting
authors.initials: C
authors.lastname: Lang
authors.forename: Munikote S
authors.initials: MS
authors.lastname: Ramanna
authors.forename: Edwin A G
authors.initials: EA
authors.lastname: van der Vossen
authors.forename: Erwin
authors.initials: E
authors.lastname: Datema
authors.forename: René Klein
authors.initials: RK
authors.lastname: Lankhorst
authors.forename: Jan
authors.initials: J
authors.lastname: de Boer
authors.forename: Sander A
authors.initials: SA
authors.lastname: Peters
authors.forename: Christian
authors.initials: C
authors.lastname: Bachem
authors.forename: Willem
authors.initials: W
authors.lastname: Stiekema
authors.forename: Richard G F
authors.initials: RG
authors.lastname: Visser
authors.forename: Hans
authors.initials: H
authors.lastname: de Jong
authors.forename: Yuling
authors.initials: Y
authors.lastname: Bai
mesh_terms: D002869:Chromosome Aberrations; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018551:Solanum lycopersicum / Q000235:genetics*; D020161:Physical Chromosome Mapping / Q000379:methods*; D011198:Solanum tuberosum / Q000235:genetics*; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
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