Efficient PCR amplification by an unnatural base pair system.
Expansion of the genetic alphabet by an unnatural base pair system enables the site-specific incorporation of extra functional components into nucleic acids and proteins. In this system, PCR amplification of DNA templates containing unnatural base pairs is essential for modern biotechnology. We present a new unnatural base pair system, in which DNA duplexes containing the unnatural base pairs can be efficiently amplified by PCR. The system also provides a method for the site-specific incorporation of functional components into amplified DNA fragments by PCR, using unnatural base substrates linked with functional groups of interest.
Kimoto
Michiko
M
Systems and Structural Biology Center, Yokohama Institute, RIKEN, Yokohama, Kanagawa, Japan.
Kawai
Rie
R
Mitsui
Tsuneo
T
Yokoyama
Shigeyuki
S
Hirao
Ichiro
I
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011725:Pyridines / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
C528341:7-(2-thienyl)imidazo(4,5-b)pyridine; D007093:Imidazoles; D011725:Pyridines; D011758:Pyrroles; D004247:DNA