Intracellular mRNA imaging with a hybridization sensitive fluorescent nucleotide.
We reported a technique for detecting intracellular RNA with a newly developed functional probe. To detect mRNAs, a polyA tail targeting probe was synthesized. This probe showed quite large enhancement in the presence of its complementary RNA. In living cultured cells, the probe also showed strong fluorescence. This technique was effective for intracellular mRNA detection.
Kubota
Takeshi
T
Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Ikeda
Shuji
S
Okamoto
Akimitsu
A
D052160:Benzothiazoles / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D015345:Oligonucleotide Probes / Q000737:chemistry*; D011806:Quinolinium Compounds / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis*; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry
D052160:Benzothiazoles; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011806:Quinolinium Compounds; D012333:RNA, Messenger; D014529:Uridine