Genetic Manipulation of Mycobacterium tuberculosis.
This unit includes protocols for the genetic manipulation of Mycobacterium tuberculosis, including nucleic acid extraction (plasmid DNA, genomic DNA, and mRNA), and methods for electroporation (transformation), transduction (including allelic exchange), and transposon mutagenesis. Considerations for working with M. tuberculosis at Biosafety Level 3 containment are also discussed.
Larsen
Michelle H
MH
Albert Einstein College of Medicine, Bronx, New York, USA.
Biermann
Karolin
K
Tandberg
Steven
S
Hsu
Tsugunda
T
Jacobs
William R
WR
D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D018274:Electroporation; D005821:Genetic Techniques; D006801:Humans; D016296:Mutagenesis; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; D004269:DNA, Bacterial