title: Basic preparative techniques for fluorescence in situ hybridization.
abstract: This unit presents protocols for preparing human metaphase chromosome slides from peripheral blood lymphocytes, isolating interphase nuclei from lymphocytes and paraffin-embedded tissues, and preparing DNA fibers. The protocols are designed so that the resulting preparations are amenable to FISH. The methods correspond to a selection of the specimens that can be analyzed with FISH techniques, and the choice of sample preparation method is highly dependent on the molecular cytogenetics question being addressed.
authors.affiliation: Leiden University, Leiden, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Wiegant
authors.forename: A K
authors.initials: AK
authors.lastname: Raap
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003582:Cytogenetics; D003584:Cytological Techniques; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008214:Lymphocytes / Q000166:cytology*
chemical_list: D005456:Fluorescent Dyes