Exceptional fluorescence and hybridization properties of a phenylpyrrolocytosine in peptide nucleic acid.
A phenylpyrrolocytosine derivative possessing bis-(ortho-aminoethoxy) substitution has been designed, synthesized and incorporated into PNA resulting in oligomers that demonstrate increased binding affinity for DNA targets. The phenylpyrrolocytosine is an exceptionally fluorescent modified nucleobase possessing a high quantum yield while the fluorescence intensity displays useful environmental sensitivity.
Wojciechowski
Filip
F
Department of Chemistry, The University of Western Ontario, London, Ontario, Canada.
Hudson
Robert H E
RH
D003596:Cytosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D011758:Pyrroles / Q000138:chemical synthesis / Q000737:chemistry*
D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids; D011758:Pyrroles; D003596:Cytosine; D004247:DNA