Efficient preparation of xanthine-containing oligodeoxynucleotide from oxanine-containing oligodeoxynucleotide, catalyzed by N alpha-acetyl-L-histidine.
Xanthine (Xan) and oxanine (Oxa) are major damage products from guanine (Gua) by NO- or HNO(2)-induced nitrosative deamination. Xan- and Oxa-containing oligodeoxynucleotides are essential substrates for the biochemical studies to reveal genotoxicity or mutagenesis raised by nitrosative oxidation. In previous study, we have developed chemical synthesis method for obtaining Oxa-ODN. Here, we proposed an efficient preparation method of Xan-ODN by incubating of Oxa-ODN in N(alpha)-acetyl-(L)-histidine (Ac-His) for 3 days at mild condition.
Doi
Akihiro
A
Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
Pack
Seung Pil
SP
Kodaki
Tsutomu
T
Makino
Keisuke
K
D002384:Catalysis; D006639:Histidine / Q000031:analogs & derivatives* / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011684:Purine Nucleosides / Q000737:chemistry*; D019820:Xanthine / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D011684:Purine Nucleosides; C468766:oxanine; D019820:Xanthine; D006639:Histidine; C023495:N-acetylhistidine