Site-specific gene manipulation of fluorescent proteins using artificial restriction DNA cutter.
Two of three amino acid residues, which compose the chromophore of the enhanced green fluorescent protein (EGFP), were converted to others by using artificial restriction DNA cutter (ARCUT). The vector prepared by ARCUT was easily connected with the insert by using oligonucleotide additive and resultant fluorescent protein such as blue fluorescent protein (BFP) was successfully expressed in cells.
Katada
Hitoshi
H
Research Center for Advanced Science and Technology, The University of Tokyo, Meguro, Tokyo 153-8904, Japan.
Ren
Yi
Y
Shigi
Narumi
N
Komiyama
Makoto
M
D019943:Amino Acid Substitution; D002563:Cerium / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics*; D020135:Peptide Nucleic Acids / Q000737:chemistry
D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D002563:Cerium; D004492:Edetic Acid; D004262:DNA Restriction Enzymes