title: Performance of indirect immunofluorescence assay, immunochromatography assay and reverse transcription-polymerase chain reaction for detecting human respiratory syncytial virus in nasopharyngeal aspirate samples.
abstract: Comparison of the use of indirect immunofluorescence assay (IFA), immunochromatography assay (ICA-BD) and reverse transcription-polymerase chain reaction (RT-PCR) for detecting human respiratory syncytial virus (HRSV) in 306 nasopharyngeal aspirates samples (NPA) was performed in order to assess their analytical performance. By comparing the results obtained using ICA-BD with those using IFA, we found relative indices of 85.0% for sensitivity and 91.2% for specificity, and the positive (PPV) and negative (NPV) predictive values were 85.0% and 91.2%, respectively. The relative indices for sensitivity and specificity as well as the PPV and NPV for RT-PCR were 98.0%, 89.0%, 84.0% and 99.0%, respectively, when compared to the results of IFA. In addition, comparison of the results of ICA-BD and those of RT-PCR yielded relative indices of 79.5% for sensitivity and 95.4% for specificity, as well as PPV and NPV of 92.9% and 86.0%, respectively. Although RT-PCR has shown the best performance, the substantial agreement between the ICA-BD and IFA results suggests that ICA-BD, also in addition to being a rapid and facile assay, could be suitable as an alternative diagnostic screening for HRSV infection in children.
authors.affiliation: Divisão de Biologia Médica, Rede Diversidade Genômica de Vírus, Instituto Adolfo Lutz, São Paulo, Brasil.
authors.forename: L R A
authors.initials: LR
authors.lastname: Vaz-de-Lima
authors.forename: M C O
authors.initials: MC
authors.lastname: Souza
authors.forename: Tk
authors.initials: T
authors.lastname: Matsumoto
authors.forename: M A
authors.initials: MA
authors.lastname: Hong
authors.forename: M M
authors.initials: MM
authors.lastname: Salgado
authors.forename: M L
authors.initials: ML
authors.lastname: Barbosa
authors.forename: N S
authors.initials: NS
authors.lastname: Sato
authors.forename: H I
authors.initials: HI
authors.lastname: Requejo
authors.forename: C A F
authors.initials: CA
authors.lastname: Oliveira
authors.forename: R
authors.initials: R
authors.lastname: Pecchini
authors.forename: E
authors.initials: E
authors.lastname: Berezin
authors.forename: S D
authors.initials: SD
authors.lastname: Passos
authors.forename: C
authors.initials: C
authors.lastname: Schvartsman
authors.forename: A
authors.initials: A
authors.lastname: Pasmanick
authors.forename: E L
authors.initials: EL
authors.lastname: Durigon
authors.forename: M
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000208:Acute Disease; D002675:Child, Preschool; D002845:Chromatography / Q000379:methods; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D017817:Nasal Lavage Fluid / Q000821:virology; D009305:Nasopharynx / Q000821:virology; D011237:Predictive Value of Tests; D012367:RNA, Viral / Q000235:genetics; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral