title: Near-infrared fluorescence detection of ATP-biotin-mediated phosphoprotein labeling.
abstract: Detection of phosphoproteins plays an important role in understanding protein function in cellular signalling pathways. Improved methods for identification and quantification of phosphoproteins are research priorities. Near-infrared (NIR) fluorescence detection of a gamma-modified ATP-biotin analog was used to detect protein phosphorylation, using both model kinase substrates and mammalian cell lysates. NIR signal intensity was dependent on substrate and ATP-biotin concentrations.
authors.affiliation: Center for Infectious Disease Research and Vaccinology, South Dakota State University, Box 2175, 1119 North Campus Drive, Brookings, SD 57007, USA.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Gao
authors.forename: Amy
authors.initials: A
authors.lastname: Schutz-Geschwender
authors.forename: Philip R
authors.initials: PR
authors.lastname: Hardwidge
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D002457:Cell Extracts; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D010455:Peptides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism; D019265:Spectroscopy, Near-Infrared; D013194:Staining and Labeling; D013379:Substrate Specificity
chemical_list: D002457:Cell Extracts; D010455:Peptides; D010750:Phosphoproteins; D001710:Biotin; D000255:Adenosine Triphosphate; D016315:Proto-Oncogene Proteins c-abl; D017868:Cyclic AMP-Dependent Protein Kinases