Monitoring demyelination and remyelination by magnetization transfer imaging in the mouse brain at 9.4 T.
OBJECTIVE
The aim of this study was to assess quantitatively structural changes in myelin content occurring during demyelination and remyelination by magnetization transfer imaging (MTI).


METHODS
In a reversible model of demyelination with no axonal loss, mice intoxicated by cuprizone were studied by MTI in vivo at 9.4 T. MRI data were compared to histopathological examinations.


RESULTS
Data revealed that the magnetization transfer ratio (MTR) decreased significantly during demyelination and increased during remyelination with strong correlation to the myelin content (r=0.79, P=0.01).


CONCLUSIONS
This study demonstrated that MTR is a sensitive and reproducible quantitative marker to assess myelin loss and repair. This may lead to in vivo monitoring of therapeutic strategies promoting remyelination.
Zaaraoui
Wafaa
W
Résonance Magnétique des Systèmes Biologiques, UMR5536 CNRS/Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat Case 93, 33076, Bordeaux Cedex, France. wzaaraoui@hotmail.com
Deloire
Mathilde
M
Merle
Michel
M
Girard
Céline
C
Raffard
Gérard
G
Biran
Marc
M
Inglese
Matilde
M
Petry
Klaus G
KG
Gonen
Oded
O
Brochet
Bruno
B
Franconi
Jean-Michel
JM
Dousset
Vincent
V
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D003471:Cuprizone / Q000633:toxicity; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D005904:Glial Fibrillary Acidic Protein; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D051379:Mice; D008810:Mice, Inbred C57BL; D004676:Myelin Basic Protein / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; C528900:glial fibrillary astrocytic protein, mouse; D003471:Cuprizone
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