Immunocytochemical detection.
This unit provides detailed descriptions for direct and indirect in situ hybridization methods. Protocols are designed for bright-field microscopy using a precipitating chromogenic substrate and for immunofluorescence detection using a hapten-labeled probe. Several alternative procedures are provided together with an excellent discussion of the strategy used for selecting a detection method.
Wiegant
J
J
Leiden University, Leiden, The Netherlands.
D000818:Animals; D005456:Fluorescent Dyes / Q000494:pharmacology; D006241:Haptens; D006801:Humans; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods*; D017403:In Situ Hybridization / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy; D008856:Microscopy, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D006241:Haptens