Determination of cardiac troponin I in the blood and heart of calves with foot-and-mouth disease.
The current study was designed to determine the changes of the cardiac troponin I (cTnI) expression in blood and tissue during the myocardial degeneration in calves with foot-and-mouth disease (FMD). Seventeen crossbred calves presenting pathological signs for FMD confirmed by viral analysis were studied. A biochemistry panel and immunohistochemistry were performed on 17 diseased calves and 7 calves used as controls. Creatine kinase (CK), CK-myocardial band (CK-MB), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities were analyzed for both groups. Cardiac troponin I levels were measured by a commercially available enzyme-linked immunosorbent assay kit. Mean cTnI (14.8 +/- 1.9 ng/ml) concentration and CK (573 +/- 407 U/l), CK-MB (238 +/- 37 U/l), AST (84 +/- 7), and LDH (298 +/- 29 U/l) activities were higher in FMD cases compared with controls. Immunohistochemistry revealed loss or depletion of cTnI expression in myocardium of all cases. None of the 7 controls showed loss of cTnI expression. Increased serum cTnI concentration correlated with myocardial injury and loss of cTnI immunolabeling in cardiomyocytes of calves with FMD.
Tunca
Recai
R
Department of Pathology, Faculty of Veterinary Medicine, University of Adnan Menderes, Isikli, Aydin, Turkey. rtunca26@hotmail.com
Sozmen
Mahmut
M
Erdogan
Hidayet
H
Citil
Mehmet
M
Uzlu
Erdogan
E
Ozen
Hasan
H
Gokçe
Erhan
E
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000453:epidemiology / Q000473:pathology*; D004196:Disease Outbreaks; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D009205:Myocarditis / Q000473:pathology / Q000662:veterinary*; D009206:Myocardium / Q000473:pathology*; D019210:Troponin I / Q000097:blood* / Q000378:metabolism*; D014421:Turkey / Q000453:epidemiology
D019210:Troponin I