Immunohistochemical analysis of low-temperature methylmethacrylate resin-embedded goat tissues.
Goats are frequently used as a suitable animal model for tissue engineering. Immunohistochemistry can be helpful in improving the understanding and evaluation of the in vivo tissue responses at a molecular level. Several commercially available antibodies (KI67, vimentin, CD31, core-binding factor alpha-1, osteocalcin, alkaline phosphatase, MAC387, CD3, CD20, CD20cy, CD79 and CD45) were evaluated on Technovit 9100 New embedded goat tissues. Only vimentin, osteocalcin, MAC387 and CD3 revealed positive staining. These antibodies can be routinely used to evaluate goat tissues at molecular level. The use and development of alternative antibodies might further supplement and complete the possibilities for immunohistochemical analysis of goat tissue samples.
Vertenten
G
G
Department of Surgery and anaesthesiology of domestic animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. geert.vertenten@ugent.be
Vlaminck
L
L
Ducatelle
R
R
Lippens
E
E
Cornelissen
M
M
Gasthuys
F
F
D000180:Acrylic Resins; D000818:Animals; D000906:Antibodies / Q000032:analysis; D015415:Biomarkers; D003080:Cold Temperature; D006041:Goats; D016591:Histocytological Preparation Techniques / Q000379:methods / Q000662:veterinary*; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D020366:Methylmethacrylate / Q000494:pharmacology*; D016610:Tissue Embedding / Q000379:methods / Q000662:veterinary*
D000180:Acrylic Resins; D000906:Antibodies; D015415:Biomarkers; D020366:Methylmethacrylate