title: Identification of Inonotus obliquus and analysis of antioxidation and antitumor activities of polysaccharides.
abstract: Inonotus obliquus, a wild wood-decay fungus which grows on Betula trees in cool climates, has a variety of biological activities that the scientific community is paying more and more attention to. However, the research work is moving at a snail's pace. The methods of strain identification and the hypha microstructure have not been reported. We isolated one strain of filamentous molds from fruit body which was collected from birch wood on Changbai Mountain, cultivated mycelia on an inclined plane, and examined its micromorphology based on macroscopic examination. The strain was identified as I. obliquus by sequencing its ITS (internal transcribed spacer) domain. We subsequently investigated some of the mycelium polysaccharides' biological activities. The strain used in this study as the producers of antioxidation and anticancer polysaccharides was LNUF008. After fermentation in a 30-L fermenter, mycelia were obtained. The polysaccharides were extracted by transonic recirculation and ethanol precipitation. In order to identify the antioxidation effect, we designed an assay to test the inhibition of endogenous and Fe(2+)-Cys-induced lipid peroxidation as well as ferrous sulfate/ascorbate (Fe(2+)-VC)-induced mitochondrial swelling. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] method was used to study the antiproliferation activity of the polysaccharides on SMMC7721 hepatoma cells. The results indicate that I. obliquus polysaccharides exhibit high antitumor and antioxidation effects. The submerged culture method of growing I. obliquus will enable large-scale production of the polysaccharides.
authors.affiliation: School of Life Science, Liaoning University, Chongshan Middle Road 66, Shenyang, 110036, People's Republic of China.
authors.forename: Yana
authors.initials: Y
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Hui
authors.forename: Wei
authors.initials: W
authors.lastname: Kou
authors.forename: Ru
authors.initials: R
authors.lastname: Xin
authors.forename: Fei
authors.initials: F
authors.lastname: Jia
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Fengqing
authors.initials: F
authors.lastname: Hu
authors.forename: Huili
authors.initials: H
authors.lastname: Zhang
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001487:Basidiomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D045744:Cell Line, Tumor; D006801:Humans; D015227:Lipid Peroxidation / Q000528:radiation effects; D051379:Mice; D008933:Mitochondrial Swelling / Q000187:drug effects; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D011134:Polysaccharides
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