Extraction of amphetamines and methylenedioxyamphetamines from urine using a monolithic silica disk-packed spin column and high-performance liquid chromatography-diode array detection.
To overcome the limitations of solid-phase extraction, we developed a device comprising a spin column packed with octadecyl silane-bonded monolithic silica for extracting amphetamines and methylenedioxyamphetamines from urine. Urine (0.5mL), buffer (0.4mL), and methoxyphenamine (internal standard) were directly put into the preactivated column. The column was centrifuged (3000rpm, 5min) for sample loading and washed. The adsorbed analytes were eluted and analyzed by high-performance liquid chromatography, without evaporation. The results were as follows: linear curves (drug concentrations of 0.2-20microg/mL); correlation coefficients >0.99; detection limit, 0.1microg/mL. The proposed method is not only useful for drugs from biological materials but also highly reproducible for the analysis of these drugs in urine.
Namera
Akira
A
Department of Forensic Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Kaumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan. namera@hiroshima-u.ac.jp
Nakamoto
Akihiro
A
Nishida
Manami
M
Saito
Takeshi
T
Kishiyama
Izumi
I
Miyazaki
Shota
S
Yahata
Midori
M
Yashiki
Mikio
M
Nagao
Masataka
M
D000662:Amphetamines / Q000302:isolation & purification / Q000652:urine*; D053000:Analytic Sample Preparation Methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D012822:Silicon Dioxide / Q000737:chemistry
D000662:Amphetamines; D012822:Silicon Dioxide; C042953:octadecyl silane-bonded silica