title: New triarylamine-based far-red DNA stainers with high two-photon absorption properties.
abstract: A series of red emitting vinyl-triphenyamines (TP) have been synthesized and evaluated for their two photon absorption (2PA) properties. These compounds are virtually non fluorescent in the free state but exhibit a bright red fluorescence upon binding to double-stranded DNA, both in one- and two-photon absorption. This feature allows one- and two-photon confocal imaging in cells of nuclear DNA with an excellent contrast. Derivatizable analogues for covalent bioconjugation to oligonucleotides are described and variation on the structure is discussed.
authors.affiliation: Institut Curie, CNRS UMR-176, Centre Universitaire, 91405 Orsay, France.
authors.forename: G
authors.initials: G
authors.lastname: Bordeau
authors.forename: E
authors.initials: E
authors.lastname: Faurel
authors.forename: C
authors.initials: C
authors.lastname: Allain
authors.forename: R
authors.initials: R
authors.lastname: Lartia
authors.forename: F
authors.initials: F
authors.lastname: Schmidt
authors.forename: C
authors.initials: C
authors.lastname: Fiorini-Debuisschert
authors.forename: F
authors.initials: F
authors.lastname: Charra
authors.forename: G
authors.initials: G
authors.lastname: Metge
authors.forename: P
authors.initials: P
authors.lastname: Tauc
authors.forename: M-P
authors.initials: MP
authors.lastname: Teulade-Fichou
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal; D017785:Photons; D011726:Pyridinium Compounds / Q000737:chemistry*
chemical_list: D000814:Aniline Compounds; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D004247:DNA