Mass spectral imaging of glycophospholipids, cholesterol, and glycophorin a in model cell membranes.
Time of flight secondary ion mass spectrometry (ToF-SIMS) and the Langmuir-Blodgett (LB) technique have been used to create and analyze reproducible membrane mimics of the inner and outer leaflets of a cellular membrane to investigate lipid-protein and lipid-lipid interactions. Films composed of phospholipids, cholesterol and an integral membrane protein were utilized. The results show the outer membrane leaflet mimic (DPPC/cholesterol/glycophorin A LB film) consisting of a single homogeneous phase whereas the inner membrane leaflet mimic (DPPE/cholesterol/glycophorin A LB film) displays heterogeneity in the form of two separate phases. A DPPE/cholesterol phase and a glycophorin A phase. This points to differences in membrane domain formation based upon the different chemical composition of the leaflets of a cell membrane. The reliability of the measurements was enhanced by establishing the influence of the matrix effect upon the measurement and by utlilizing PCA to enhance the contrast of the images.
Baker
Matthew J
MJ
Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, M1 7DN, U.K. M.J.Baker@manchester.ac.uk
Zheng
Leiliang
L
Winograd
Nicholas
N
Lockyer
Nicholas P
NP
Vickerman
John C
JC
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism*; D002784:Cholesterol / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry*; D006021:Glycophorins / Q000737:chemistry*; D007477:Ions; D008055:Lipids / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008566:Membranes / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D025341:Principal Component Analysis; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties
D006017:Glycolipids; D006021:Glycophorins; D007477:Ions; D008055:Lipids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D011506:Proteins; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; D002784:Cholesterol
Science. 1977 Feb 25;195(4280):743-53
402030
Anal Chem. 2005 Oct 1;77(19):6190-6
16194078
Rapid Commun Mass Spectrom. 1998;12(18):1246-52
9772767
Langmuir. 2005 Feb 1;21(3):807-13
15667151
Anal Chem. 2006 Jun 1;78(11):3651-8
16737220
Biomaterials. 2007 May;28(15):2412-23
17335898
J Clin Invest. 2002 Sep;110(5):597-603
12208858
Neurology. 2001 Sep 25;57(6):1089-93
11571339
Appl Surf Sci. 2006 Jul;252(19):6716-6718
18079999
Science. 1972 Feb 18;175(4023):720-31
4333397
Anal Chem. 2002 Nov 15;74(22):5711-6
12463353
Langmuir. 2007 May 8;23(10):5645-50
17417886
Chem Phys Lipids. 1994 Sep 6;73(1-2):139-58
8001179
Biophys J. 2000 Jul;79(1):357-69
10866961
Biophys J. 2006 Aug 15;91(4):1347-56
16632503
Anal Chem. 2002 Apr 15;74(8):1824-35
11985314