title: Induction of apoptosis by KI0477959 through activation of caspase-3 in human leukemia cell line, HL-60 cells.
abstract: KI0477959 (Herbkines) has been used for the purpose of development of physical strength in wasting diseases, like cancer. In the present study, apoptosis-inducing activities of butanol fraction of KI0477959 were studied in human leukemia cell line, HL-60 cells. KI0477959 increased cytotoxicity but had less effect on human peripheral blood mononuclear cells. KI0477959-induced apoptosis was accompanied by activation of caspase-3 and specific proteolytic cleavage of poly-ADP-ribose polymerase. Increased apoptosis was reduced by treatment with p38 and extracellular signal-regulated protein kinase (ERK) inhibitors. These results suggest that KI0477959 induces apoptosis through activation of caspase-3, p38, and ERK in HL-60 cells.
authors.affiliation: Biochip Research Center, Hoseo University, Chungnam, South Korea.
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Joon-Bae
authors.initials: JB
authors.lastname: Kim
authors.forename: Hoyeon
authors.initials: H
authors.lastname: Go
authors.forename: Seong-Gyu
authors.initials: SG
authors.lastname: Ko
authors.forename: Young-Wan
authors.initials: YW
authors.lastname: Seo
authors.forename: Sejin
authors.initials: S
authors.lastname: Jeong
authors.forename: Jinhan
authors.initials: J
authors.lastname: Park
authors.forename: Ho-Jeong
authors.initials: HJ
authors.lastname: Na
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
mesh_terms: D000704:Analysis of Variance; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024161:Collagen Type XI / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology; D055670:Medicine, Korean Traditional; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors
chemical_list: C499319:COL11A2 protein, human; D024161:Collagen Type XI; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C488392:Herbkines; D010936:Plant Extracts; D020929:Mitogen-Activated Protein Kinase Kinases; D053148:Caspase 3