title: Detection of respiratory pathogens in air samples from acutely infected pigs.
abstract: Pathogens causing significant respiratory disease in growing pigs include Porcine reproductive and respiratory syndrome virus, Porcine circovirus 2, swine influenza virus, porcine respiratory coronavirus, Mycoplasma hyopneumoniae, and Bordetella bronchiseptica. The objective of this research was to characterize the respiratory excretion of these pathogens by acutely infected pigs. Pigs were inoculated under experimental conditions with 1 pathogen. Samples were collected from the upper respiratory tract and exhaled air. All pathogens were detected in swabs of the upper respiratory tract, but only M. hyopneumoniae and B. bronchiseptica were detected in expired air from individually sampled, acutely infected pigs. These findings suggest either that the acutely infected pigs did not aerosolize the viruses or that the quantity of virus excreted was below the detection threshold of current sampling or assay systems, or both, at the individual-pig level.
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1250, USA.
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Hermann
authors.forename: Susan L
authors.initials: SL
authors.lastname: Brockmeier
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Yoon
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Zimmerman
mesh_terms: D000391:Air Microbiology; D000818:Animals; D016950:Bordetella bronchiseptica / Q000302:isolation & purification / Q000472:pathogenicity; D002353:Carrier State; D017925:Circovirus / Q000302:isolation & purification / Q000472:pathogenicity; D004197:Disease Reservoirs / Q000382:microbiology / Q000662:veterinary / Q000821:virology; D009980:Influenza A virus / Q000302:isolation & purification / Q000472:pathogenicity; D009055:Mouth / Q000382:microbiology* / Q000821:virology; D045705:Mycoplasma hyopneumoniae / Q000302:isolation & purification / Q000472:pathogenicity; D009296:Nasal Cavity / Q000382:microbiology* / Q000821:virology; D045722:Porcine Respiratory Coronavirus / Q000302:isolation & purification / Q000472:pathogenicity; D019316:Porcine respiratory and reproductive syndrome virus / Q000302:isolation & purification / Q000472:pathogenicity; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology* / Q000821:virology
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