title: Toward a prostate specific antigen-based prostate cancer diagnostic assay: preparation of keyhole limpet hemocyanin-conjugated normal and transformed prostate specific antigen fragments.
abstract: Prostate specific antigen (PSA) molecules secreted by cancerous and normal prostate cells differ in their N-linked glycan composition, while the peptide backbone appears to be conserved. Antibodies selectively recognizing such differentially glycosylated PSA structures could form a basis for a new diagnostic assay for prostate cancer. Twenty-amino acid PSA fragments carrying di-, tri-, and tetrabranched complex-type glycans were prepared by total synthesis and conjugated to maleimide-modified keyhole limpet hemocyanin (KLH) carrier protein through backbone Cys residues. These glycopeptide/KLH conjugates were then used for antibody generation.
authors.affiliation: Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA.
authors.forename: Vadim Y
authors.initials: VY
authors.lastname: Dudkin
authors.forename: Justin S
authors.initials: JS
authors.lastname: Miller
authors.forename: Anna S
authors.initials: AS
authors.lastname: Dudkina
authors.forename: Christophe
authors.initials: C
authors.lastname: Antczak
authors.forename: David A
authors.initials: DA
authors.lastname: Scheinberg
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Danishefsky
mesh_terms: D000906:Antibodies / Q000276:immunology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006433:Hemocyanins / Q000737:chemistry*; D006801:Humans; D008297:Male; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D010446:Peptide Fragments; D006433:Hemocyanins; D017430:Prostate-Specific Antigen
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