title: Effects of grape seed proanthocyanidin extracts on peripheral nerves in streptozocin-induced diabetic rats.
abstract: Diabetic peripheral neuropathy (DPN) is one of the most common diabetic chronic complications. The aim of this study was to clarify whether grape seed proanthocyanidins extracts (GSPE) are therapeutic agents against DPN. In this study, we used streptozocin (STZ) to induce diabetic rats. GSPEs (250 mg/kg body weight/d) were administrated to diabetic rats for 24 wk. Motor nerve conductive velocity (MNCV) and mechanical hyperalgesia were determined in the rats. Serum glucose, glycated hemoglobin, advanced glycation end products (AGEs), and tissue malondialdehyde (MDA) and superoxide dismutase (SOD) were determined. Light and electron microscopy were used to observe the changes of nerval ultrastructure.GSPE significantly increased the MNCV, mechanical hyperalgesia and SOD of diabetic rats (p<0.05) and reduced the AGEs and MDA of diabetic rats (p<0.05). After being treated by GSPE, the severe segmental demyelination was decreased and Schwann cells were improved. In conclusion, GSPE plays an important role against DPN. With the decreasing of AGEs and MDA, it can ameliorate oxidation-associated nerval damage. This study may provide a new recognition of natural medicine for the treatment of DPN.
authors.affiliation: Department of Geriatrics, Qi-Lu Hospital of Shandong University, Jinan, Shandong Province 250012, the People's Republic of China.
authors.forename: Xiao-pei
authors.initials: XP
authors.lastname: Cui
authors.forename: Bao-ying
authors.initials: BY
authors.lastname: Li
authors.forename: Hai-qing
authors.initials: HQ
authors.lastname: Gao
authors.forename: Na
authors.initials: N
authors.lastname: Wei
authors.forename: Wei-ling
authors.initials: WL
authors.lastname: Wang
authors.forename: Mei
authors.initials: M
authors.lastname: Lu
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000473:pathology; D003929:Diabetic Neuropathies / Q000188:drug therapy* / Q000473:pathology; D056604:Grape Seed Extract; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D017288:Pain Threshold / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects* / Q000473:pathology; D027843:Vitis
chemical_list: D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D010936:Plant Extracts; D044945:Proanthocyanidins