Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria.
OBJECTIVE
The design and evaluation of an oligonucleotide microarray in order to detect and identify viable bacterial species that play a significant role in beer spoilage. These belong to the species of the genera Lactobacillus, Megasphaera, Pediococcus and Pectinatus.


RESULTS
Oligonucleotide probes specific to beer spoilage bacteria were designed. In order to detect viable bacteria, the probes were designed to target the intergenic spacer regions (ISR) between 16S and 23S rRNA. Prior to hybridization the ISR were amplified by combining reverse transcriptase and polymerase chain reactions using a designed consenus primer. The developed oligonucleotide microarrays allows the detection of viable beer spoilage bacteria.


CONCLUSIONS
This method allows the detection and discrimination of single bacterial species in a sample containing complex microbial community. Furthermore, microarrays using oligonucleotide probes targeting the ISR allow the distinction between viable bacteria with the potential to grow and non growing bacteria.


CONCLUSIONS
The results demonstrate the feasibility of oligonucleotide microarrays as a contamination control in food industry for the detection and identification of spoilage micro-organisms within a mixed population.
Weber
D G
DG
Institute of Technical Microbiology, Hamburg University of Technology, Hamburg, Germany.
Sahm
K
K
Polen
T
T
Wendisch
V F
VF
Antranikian
G
G
D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D001515:Beer / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D019649:Food Industry; D005516:Food Microbiology; D020869:Gene Expression Profiling / Q000379:methods*; D007778:Lactobacillus / Q000235:genetics; D045851:Megasphaera / Q000235:genetics; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D045852:Pectinatus / Q000235:genetics; D010376:Pediococcus / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004269:DNA, Bacterial; D021901:DNA, Intergenic