Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium.
Epidemiological and experimental data point to involvement of oxygen derived radicals in the pathogenesis of gynecological disorders, as well as in cancer development. The objective of the present study was to examine changes in activities and levels of copper/zinc superoxide dismutase (CuZnSOD) and lipid hydroperoxides (LOOH) in blood and endometrial tissue of patients diagnosed with uterine myoma, endometrial polypus, hyperplasia simplex, hyperplasia complex and adenocarcinoma endometrii. The results of our study have shown decreased SOD activities and unchanged SOD protein level in blood of all examined patients in comparison to healthy subjects. Decrease of both SOD activity and level was found in endometrium of patients with hyperplasia simplex, hyperplasia complex and adenocarcinoma in comparison to women with polypus or myoma. LOOH level was elevated in both tissues of patients with hyperplasia or adenocarcinoma in comparison to healthy subjects or patients with benign diagnosis. Our findings suggest that the decrease in SOD activity and level, as well as the increase in LOOH level, in patients with gynecological disorders, render these patients more susceptible to oxidative damage caused by reactive oxygen species (ROS). An imbalance in ROS formation and SOD level may be important in the pathogenesis and/or perpetuation of tissue damage in gynecological patients. Since evidence suggests that SOD may be a therapy target for cancer treatment, our findings provide a basis for further research and options for clinical applications.
Pejić
Snezana
S
Laboratory of Molecular Biology and Endocrinology, Vinca Institute of Nuclear Sciences, Belgrade, Serbia. snezana@vin.bg.ac.yu
Todorović
Ana
A
Stojiljković
Vesna
V
Cvetković
Dragana
D
Lucić
Nenad
N
Radojicić
Ratko M
RM
Saicić
Zorica S
ZS
Pajović
Snezana B
SB
D000230:Adenocarcinoma / Q000097:blood / Q000201:enzymology; D014408:Biomarkers, Tumor / Q000032:analysis; D004714:Endometrial Hyperplasia / Q000097:blood / Q000201:enzymology; D016889:Endometrial Neoplasms / Q000097:blood / Q000201:enzymology; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000097:blood / Q000201:enzymology; D008054:Lipid Peroxides / Q000032:analysis*; D008875:Middle Aged; D011127:Polyps / Q000097:blood / Q000201:enzymology; D013482:Superoxide Dismutase / Q000032:analysis*; D014594:Uterine Neoplasms / Q000097:blood / Q000201:enzymology
D014408:Biomarkers, Tumor; D008054:Lipid Peroxides; D013482:Superoxide Dismutase