title: Subsites for substrate recognition by bacterial ribonuclease P.
abstract: We have prepared series of shape variant RNAs of a tRNA precursor and analyzed the substrate shape recognition by bacterial ribonuclease P ribozyme and holoenzyme. The results showed the evidence for the presence of subsites for the recognition of the trna shape. We will discuss a new model for substrate recognition and the role of the protein component.
authors.affiliation: Tempakucho, Toyohashi, Aichi 441-8580, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Fujimoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suwa
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nagai
authors.forename: Yo
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: Terumichi
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D001483:Base Sequence; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D020035:Holoenzymes / Q000737:chemistry; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000737:chemistry / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism; D043262:Ribonuclease P / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D029968:Escherichia coli Proteins; D020035:Holoenzymes; D012322:RNA Precursors; D012343:RNA, Transfer; D043262:Ribonuclease P; C470865:ribonuclease P, E coli