title: Diversity in Paracoccidioides brasiliensis. The PbGP43 gene as a genetic marker.
abstract: Paracoccidioides brasiliensis is a temperature-dependent dimorphic fungus and the agent of paracoccidioidomycosis (PCM), which is prevalent in rural workers of Latin American countries. Until a decade ago, most of the studies involving P. brasiliensis used clinical isolates, since environmental samples from soil are difficult to obtain. More recently, P. brasiliensis has been isolated from infected wild and domestic animals, especially from the nine-banded armadillo Dasypus novemcinctus in Brazil. Over the years, diversity within the species has been observed at several phenotypic levels. The present review will discuss the reports focusing on genetic polymorphism, which culminated with the detection of P. brasiliensis phylogenetic species as a result of a multilocus study. Polymorphism in the PbGP43 gene is detailed. This gene encodes fungal glycoprotein gp43, a dominant P. brasiliensis antigen largely studied in the last two decades for its importance in diagnosis, immune protection, and adhesive properties to extracellular matrix-associated proteins. Fungal traits associated with genetic groups are discussed.
authors.affiliation: Cell Biology Division, Federal University of São Paulo, UNIFESP, Rua Botucatu, 862, oitavo andar, São Paulo, SP 04023-062, Brazil. rpuccia@unifesp.br
authors.forename: Rosana
authors.initials: R
authors.lastname: Puccia
authors.forename: Juan G
authors.initials: JG
authors.lastname: McEwen
authors.forename: Patrícia S
authors.initials: PS
authors.lastname: Cisalpino
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D010228:Paracoccidioides / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: C103180:43 kDa protein, Paracoccidioides; D000946:Antigens, Fungal; D005656:Fungal Proteins; D005819:Genetic Markers; D006023:Glycoproteins
references.citation: Antimicrob Agents Chemother. 2006 Aug;50(8):2814-9
references.pmid: 16870776
references.citation: Proc Natl Acad Sci U S A. 1997 May 13;94(10):5478-82
references.pmid: 9144263
references.citation: Microbiology (Reading). 1994 May;140 ( Pt 5):1189-94
references.pmid: 8025684
references.citation: Mol Biol Evol. 2006 Jan;23(1):65-73
references.pmid: 16151188
references.citation: J Clin Microbiol. 1994 Oct;32(10):2377-81
references.pmid: 7814469
references.citation: J Med Vet Mycol. 1997 May-Jun;35(3):205-11
references.pmid: 9229337
references.citation: J Med Vet Mycol. 1996 Jun-Jul;34(3):181-6
references.pmid: 8803798
references.citation: Clin Exp Immunol. 1994 Jul;97(1):113-9
references.pmid: 8033408
references.citation: J Med Vet Mycol. 1995 Nov-Dec;33(6):379-83
references.pmid: 8683406
references.citation: Mycopathologia. 1990 Jan;109(1):13-7
references.pmid: 2139178
references.citation: Science. 1972 Jul 28;177(4046):368-9
references.pmid: 5035491
references.citation: Mol Ecol. 2003 Dec;12(12):3383-401
references.pmid: 14629354
references.citation: Mol Med. 2003 Sep-Dec;9(9-12):209-19
references.pmid: 15208742
references.citation: Med Mycol. 2003 Apr;41(2):89-96
references.pmid: 12964840
references.citation: Glycobiology. 1996 Jul;6(5):507-15
references.pmid: 8877371
references.citation: Microbiology (Reading). 1997 Jan;143 ( Pt 1):197-202
references.pmid: 9025294
references.citation: Med Mycol. 2000 Jun;38(3):185-91
references.pmid: 10892985
references.citation: Vaccine. 2000 Jul 1;18(26):3050-8
references.pmid: 10825609
references.citation: Med Mycol. 1999 Jun;37(3):219-22
references.pmid: 10421855
references.citation: J Med Vet Mycol. 1986 Dec;24(6):445-54
references.pmid: 3572679
references.citation: Microbes Infect. 2005 Jan;7(1):55-65
references.pmid: 15716071
references.citation: J Clin Microbiol. 2003 Dec;41(12):5735-7
references.pmid: 14662970
references.citation: Genome Res. 2000 Dec;10(12):1865-77
references.pmid: 11116083
references.citation: Rev Inst Med Trop Sao Paulo. 2005 Sep-Oct;47(5):257-62
references.pmid: 16302108
references.citation: Infect Genet Evol. 2006 Sep;6(5):344-51
references.pmid: 16473563
references.citation: Hybridoma. 1996 Dec;15(6):415-22
references.pmid: 8985752
references.citation: Yeast. 2001 Jul;18(10):981-8
references.pmid: 11447604
references.citation: Mycopathologia. 1985 Dec;92(3):173-8
references.pmid: 4088290
references.citation: Infect Immun. 1986 Jul;53(1):199-206
references.pmid: 2424841
references.citation: J Clin Microbiol. 1995 Feb;33(2):505-7
references.pmid: 7714219
references.citation: Fungal Genet Biol. 2007 May;44(5):347-56
references.pmid: 17166750
references.citation: J Biol Chem. 1996 Feb 23;271(8):4553-60
references.pmid: 8626811
references.citation: Fungal Genet Biol. 2003 Jun;39(1):60-9
references.pmid: 12742064
references.citation: Mycopathologia. 1987 Aug;99(2):119-28
references.pmid: 3657902
references.citation: Mycopathologia. 1990 Sep;111(3):173-80
references.pmid: 2233986
references.citation: J Clin Microbiol. 1998 Mar;36(3):742-7
references.pmid: 9508305
references.citation: J Gen Microbiol. 1980 Mar;117(1):147-54
references.pmid: 7391815
references.citation: Fungal Genet Biol. 2007 May;44(5):357-67
references.pmid: 17331762
references.citation: Mem Inst Oswaldo Cruz. 2006 May;101(3):331-4
references.pmid: 16862332
references.citation: J Med Vet Mycol. 1991;29(4):243-53
references.pmid: 1719178
references.citation: J Clin Microbiol. 1998 Jun;36(6):1733-6
references.pmid: 9620409
references.citation: Genetics. 2004 Nov;168(3):1231-48
references.pmid: 15579682
references.citation: J Clin Microbiol. 2003 Jul;41(7):2849-54
references.pmid: 12843011
references.citation: Mycopathologia. 1997;140(1):19-27
references.pmid: 9608721
references.citation: J Biol Chem. 1997 Feb 7;272(6):3161-7
references.pmid: 9013549
references.citation: Trends Microbiol. 1993 Dec;1(9):338-42
references.pmid: 8162423
references.citation: Am J Trop Med Hyg. 1990 Aug;43(2):200-6
references.pmid: 2389824
references.citation: J Med Vet Mycol. 1987 Jun;25(3):165-75
references.pmid: 3612432
references.citation: Fungal Genet Biol. 2006 Jun;43(6):401-9
references.pmid: 16631397
references.citation: Rev Inst Med Trop Sao Paulo. 1992 Nov-Dec;34(6):511-6
references.pmid: 1342119
references.citation: FEMS Immunol Med Microbiol. 2002 Jul 12;33(3):151-7
references.pmid: 12110476
references.citation: Fungal Genet Biol. 2007 Dec;44(12):1387-98
references.pmid: 17512227
references.citation: J Clin Microbiol. 2001 Jul;39(7):2683-6
references.pmid: 11427596
references.citation: J Clin Microbiol. 2006 Jun;44(6):2153-7
references.pmid: 16757613
references.citation: J Biol Chem. 2005 Jul 1;280(26):24706-14
references.pmid: 15849188
references.citation: J Clin Microbiol. 2004 Nov;42(11):5007-14
references.pmid: 15528688
references.citation: Fungal Genet Biol. 1997 Apr;21(2):223-7
references.pmid: 9228790
references.citation: J Med Vet Mycol. 1997 Jan-Feb;35(1):13-8
references.pmid: 9061580
references.citation: Rev Inst Med Trop Sao Paulo. 1999 Sep-Oct;41(5):279-83
references.pmid: 10602541
references.citation: Infect Immun. 1998 Feb;66(2):786-93
references.pmid: 9453642
references.citation: Sabouraudia. 1984;22(3):255-7
references.pmid: 6463809
references.citation: Nat Rev Genet. 2004 Jun;5(6):435-45
references.pmid: 15153996
references.citation: Mycopathologia. 1998-1999;143(3):165-9
references.pmid: 10353214
references.citation: J Clin Microbiol. 2000 Nov;38(11):3960-6
references.pmid: 11060052
references.citation: Microbes Infect. 2006 May;8(6):1550-9
references.pmid: 16698299
references.citation: J Clin Microbiol. 1991 Aug;29(8):1610-5
references.pmid: 1722220
references.citation: Eukaryot Cell. 2003 Feb;2(1):34-48
references.pmid: 12582121
references.citation: Med Mycol. 1999 Aug;37(4):269-76
references.pmid: 10421862
references.citation: J Mol Biol. 1999 Dec 3;294(3):771-83
references.pmid: 10610795
references.citation: Med Mycol. 2000 Dec;38(6):437-41
references.pmid: 11204881
references.citation: FEMS Microbiol Rev. 2007 Mar;31(2):168-92
references.pmid: 17313520
references.citation: Mycopathologia. 1998-1999;144(2):61-5
references.pmid: 10481285
references.citation: J Med Vet Mycol. 1995 Jul-Aug;33(4):223-7
references.pmid: 8531019
references.citation: Med Mycol. 2000 Aug;38(4):317-22
references.pmid: 10975700
references.citation: Science. 1967 Apr 28;156(3774):528-9
references.pmid: 6021679
references.citation: Fungal Genet Biol. 2007 Jan;44(1):25-31
references.pmid: 16879998
references.citation: Yeast. 2003 Feb;20(3):263-71
references.pmid: 12557278
references.citation: J Med Vet Mycol. 1995 Sep-Oct;33(5):281-5
references.pmid: 8544079
references.citation: Mutat Res. 2006 Nov-Dec;613(2-3):76-102
references.pmid: 16979375