title: Efficient PCR amplification by an unnatural base pair system.
abstract: Expansion of the genetic alphabet by an unnatural base pair system enables the site-specific incorporation of extra functional components into nucleic acids and proteins. In this system, PCR amplification of DNA templates containing unnatural base pairs is essential for modern biotechnology. We present a new unnatural base pair system, in which DNA duplexes containing the unnatural base pairs can be efficiently amplified by PCR. The system also provides a method for the site-specific incorporation of functional components into amplified DNA fragments by PCR, using unnatural base substrates linked with functional groups of interest.
authors.affiliation: Systems and Structural Biology Center, Yokohama Institute, RIKEN, Yokohama, Kanagawa, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Kimoto
authors.forename: Rie
authors.initials: R
authors.lastname: Kawai
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Mitsui
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hirao
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011725:Pyridines / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: C528341:7-(2-thienyl)imidazo(4,5-b)pyridine; D007093:Imidazoles; D011725:Pyridines; D011758:Pyrroles; D004247:DNA