The use of spent brewery grains for Pleurotus ostreatus cultivation and enzyme production.
In the brewing industry, spent brewery grains (SBGs) are byproducts with a low economic value. The potential use of this leftover as a substrate ingredient for Pleurotus ostreatus fruiting body cultivation and enzyme production was evaluated. The best substrate mixture for P. ostreatus mycelium growth comprised 30% wheat bran (WB), 68% beech sawdust (BS) and 2% CaCO3. On the substrates containing SBG, the fastest mycelium growth was observed on the substrate composed of 10% SBG, 20% WB, 68% BS and 2% CaCO3. The highest biological efficiency (51%) of fruiting bodies was determined on the mixtures containing 20% WB, 10% SBG and 2% CaCO3. The SBGs with the addition of WB were also shown to be suitable as a substrate for enzyme production. However, the supplementation levels designate which enzymes are produced and in what amounts.
Gregori
Andrej
A
Scientific Research Centre Bistra Ptuj, Slovenski trg 6, 2250 Ptuj, Slovenia. andrej.gregori@gmail.com
Svagelj
Mirjan
M
Pahor
Bojan
B
Berovic
Marin
M
Pohleven
Franc
F
D001515:Beer / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002523:Edible Grain / Q000382:microbiology*; D007220:Industrial Waste / Q000517:prevention & control; D010544:Peroxidases / Q000378:metabolism*; D020076:Pleurotus / Q000201:enzymology* / Q000254:growth & development*
D007220:Industrial Waste; D010544:Peroxidases