Boosting tandem affinity purification of plant protein complexes.
Protein-interaction mapping based on the tandem affinity purification (TAP) approach has been successfully established for several systems, such as yeast and mammalian cells. However, relatively few protein complex purifications have been reported for plants. Here, we highlight solutions for the pitfalls and propose a major breakthrough in the quest for a better TAP tag in plants.
Van Leene
Jelle
J
Department of Plant Systems Biology, Flanders Institute for Biotechnology and Department of Molecular Genetics, Ghent University, Technologiepark 927, 9052 Gent, Belgium.
Witters
Erwin
E
Inzé
Dirk
D
De Jaeger
Geert
G
D001426:Bacterial Proteins / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry; D010450:Endopeptidases / Q000737:chemistry; D010940:Plant Proteins / Q000302:isolation & purification*; D025941:Protein Interaction Mapping / Q000379:methods*
D001426:Bacterial Proteins; D002352:Carrier Proteins; C034284:IgG Fc-binding protein, Streptococcus; D010940:Plant Proteins; C452700:streptavidin-binding peptide; D010450:Endopeptidases; C099488:TEV protease