Genomic instability demonstrates similarity between DCIS and invasive carcinomas.
OBJECTIVE
To assess telomere DNA content (TC) and the number of sites of allelic imbalance (AI) as a function of breast cancer progression.


METHODS
TC and AI were determined in 54 histologically normal tissues, 10 atypical ductal hyperplasias (ADH), 122 in situ ductal carcinomas (DCIS) and 535 invasive carcinomas (Stage I-IIIA).


RESULTS
TC was altered in ADH lesions (20%), DCIS specimens (53%) and invasive carcinomas (51%). The mean number of sites of AI was 0.26 in histologically normal group tissue, increased to 1.00 in ADH, 2.94 in DCIS, and 3.07 in invasive carcinomas. All groups were statistically different from the histologically normal group (P < 0.001 for each); however, there was no difference between DCIS and the invasive groups.


CONCLUSIONS
Genomic instability increases in ADH and plateaus in DCIS without further increase in the invasive carcinomas, supporting the notion that invasive carcinomas evolve from or in parallel with DCIS.
Heaphy
Christopher M
CM
Department of Biochemistry and Molecular Biology, University of New Mexico, Albuquerque, 87131-0001, USA.
Bisoffi
Marco
M
Joste
Nancy E
NE
Baumgartner
Kathy B
KB
Baumgartner
Richard N
RN
Griffith
Jeffrey K
JK
D000293:Adolescent; D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002278:Carcinoma in Situ / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000473:pathology; D005260:Female; D042822:Genomic Instability; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D016615:Telomere / Q000235:genetics; D055815:Young Adult
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