New eximer-based tandem systems for SNP detection.
We report here the design and synthesis of new mono- and bis-pyrene-labeled oligo(2'-O-methylribonucleotide) tandems as perspective probes for SNP detection. The detection strategy is based on the eximer formation when two or more pyrene groups are brought into close proximity upon hybridization of the tandem components with DNA. The potential of SNP detection with tandems of pyrene-labeled oligo(2'-O-methylribonucleotides) by duplex melting curve analysis based on excimer fluorescence registration was demonstrated.
Novopashina
Daria S
DS
Institute of Chemical Biology and Fundamental Medicine SB RAS, 8 Lavrentiev ave., Novosibirsk 630090, Russia. danov@niboch.nsc.ru
Meschaninova
Maria I
MI
Kholodar
Svetlana A
SA
Lomzov
Alexander A
AA
Venyaminova
Alya G
AG
D005456:Fluorescent Dyes / Q000737:chemistry*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; D042965:Methylenetetrahydrofolate Reductase (NADPH2)