Click chemistry and oligonucleotides: how a simple reaction can do so much.
Copper catalyzed Alkyne Azide 1,3-dipolar cycloaddition (CuAAC) "click reaction" was applied for the construction of oligonucleotide conjugates, circular objects and phosphodiester glyco-clusters. To this end, several strategies were developed to introduce either alkyne or azide functions into an oligonucleotide.
Morvan
François
F
IBMM, UMR5247 CNRS Université Montpellier 1, Université Montpellier 2, Place E. Bataillon, CC1704, 34095 Montpellier Cedex 5, France. morvan@univ-montp2.fr
Meyer
Albert
A
Pourceau
Gwladys
G
Vidal
Sébastien
S
Chevolot
Yann
Y
Souteyrand
Eliane
E
Vasseur
Jean-Jacques
JJ
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010455:Peptides / Q000737:chemistry
D000480:Alkynes; D001386:Azides; D002241:Carbohydrates; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D010455:Peptides; C434331:phosphoramidite; D003300:Copper