Interactions between M protein and other structural proteins of severe, acute respiratory syndrome-associated coronavirus.
Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) structural proteins (S, E, M, and NC) localize in different subcellular positions when expressed individually. However, SARS-CoV M protein is co-localized almost entirely with S, E, or NC protein when co-expressed in the cells. On the other hand, only partial co-localization was observed when S and E, S and NC, or E and NC were co-expressed in the cells. Interactions between SARS-CoV M and other structural proteins but not interactions between S and E, S and NC, or E and NC were further demonstrated by co-immunoprecipitation assay. These results indicate that SARS-CoV M protein, similar to the M proteins of other coronaviruses, plays a pivotal role in virus assembly. The cytoplasmic C-terminus domain of SARS-CoV M protein was responsible for binding to NC protein. Multiple regions of M protein interacted with E and S proteins. A model for the interactions between SARS-CoV M protein and other structural proteins is proposed. This study helps us better understand protein-protein interactions during viral assembly of SARS-CoV.
Hsieh
Yi-Ching
YC
Graduate Institute of Molecular and Cellular Biology, Tzu Chi University, 701, Section 3, Chung Yang Road, Hualien, Taiwan.
Li
Hui-Chun
HC
Chen
Shih-Chi
SC
Lo
Shih-Yen
SY
D000818:Animals; D002522:Chlorocebus aethiops; D000086862:Coronavirus M Proteins; D017353:Gene Deletion; D011485:Protein Binding; D025941:Protein Interaction Mapping; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000378:metabolism*; D045169:Severe Acute Respiratory Syndrome / Q000378:metabolism / Q000821:virology*; D014709:Vero Cells; D014763:Viral Matrix Proteins / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000378:metabolism*
D000086862:Coronavirus M Proteins; C000706237:M protein, SARS-CoV; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins
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