Properties of polyethylene glycol supported tetraarylporphyrin in aqueous solution and its interaction with liposomal membranes.
5,10,15,20-Tetrakis(4-hydroxyphenyl)porphyrin was functionalized by covalent attachment of poly(ethylene glycol) (PEG) chains of various molecular weights, 350, 2000, and 5000 Da. The properties of PEG-functionalized tetraarylporphyrins in aqueous solution and their interactions with liposomes have been studied. Electronic absorption spectroscopy, dynamic light scattering, atomic force microscopy, and fluorescence quenching were used to monitor aggregation of porphyrin chromophores and behavior of the attached PEG chains in the aqueous solution. The tendency for aggregation of porphyrin chromophores in aqueous solution and the efficiency of fluorescence quenching by KI decrease with increasing length of PEG chain linked to the porphyrin ring. The experimental results indicate that polymer clusters are present in aqueous solution of all pegylated porphyrins. The interactions between the pegylated porphyrins and phosphatidylcholine liposomes in the aqueous solution were studied using the fluorescence methods. The apparent binding constants of porphyrin chromophores to liposomes were determined. The degree of binding was found to be dependent on the molecular weight of the attached polymer.
Nawalany
Kinga
K
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
Kozik
Bartlomiej
B
Kepczynski
Mariusz
M
Zapotoczny
Szczepan
S
Kumorek
Marta
M
Nowakowska
Maria
M
Jachimska
Barbara
B
D000042:Absorption; D004337:Drug Carriers / Q000737:chemistry; D008027:Light; D008081:Liposomes / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D011092:Polyethylene Glycols / Q000737:chemistry*; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions; D014867:Water / Q000737:chemistry*
D004337:Drug Carriers; D008081:Liposomes; D008567:Membranes, Artificial; D011166:Porphyrins; D012996:Solutions; D014867:Water; D011092:Polyethylene Glycols; C051232:5,10,15,20-tetra(4-hydroxyphenyl)porphyrin