title: Various spatiotemporal expression profiles of anther-expressed genes in rice.
abstract: The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation.
authors.affiliation: Bioscience and Biotechnology Center, Nagoya University, Furocho, Chikusa, Nagoya, 464-8601 Japan.
authors.forename: Tokunori
authors.initials: T
authors.lastname: Hobo
authors.forename: Keita
authors.initials: K
authors.lastname: Suwabe
authors.forename: Koichiro
authors.initials: K
authors.lastname: Aya
authors.forename: Go
authors.initials: G
authors.lastname: Suzuki
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yano
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ishimizu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fujita
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Kazuki
authors.initials: K
authors.lastname: Hamada
authors.forename: Masumi
authors.initials: M
authors.lastname: Miyano
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Fujioka
authors.forename: Fumi
authors.initials: F
authors.lastname: Kaneko
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Kazama
authors.forename: Yoko
authors.initials: Y
authors.lastname: Mizuta
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Takahashi
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Shiono
authors.forename: Mikio
authors.initials: M
authors.lastname: Nakazono
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Tsutsumi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nagamura
authors.forename: Nori
authors.initials: N
authors.lastname: Kurata
authors.forename: Masao
authors.initials: M
authors.lastname: Watanabe
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsuoka
mesh_terms: D016000:Cluster Analysis; D005718:Gametogenesis / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D007834:Lasers; D042282:Microdissection / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D011058:Pollen / Q000235:genetics* / Q000254:growth & development; D018749:RNA, Plant / Q000235:genetics
chemical_list: D018749:RNA, Plant
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