Cyanidin 3-O-glucopyranoside protects and rescues SH-SY5Y cells against amyloid-beta peptide-induced toxicity.
The amyloid-beta (A beta) peptide (1-42) aggregation into oligomeric and fibrillar species affects neuronal viability, having a causal role in the development of Alzheimer's disease. Among dietary anthocyanins, cyanidin 3-O-glucoside (Cy-3G) and its metabolites, such as protocatechuic acid (PA), have gained attention as potential neuroprotective agents. In this in-vitro study, we demonstrated that Cy-3G, but not PA, can inhibit A beta1-42 spontaneous aggregation using thioflavin T fluorescence assay and transmission electron microscopy. Furthermore, treatment of human neuronal SH-SY5Y cells with Cy-3G during oligomeric and fibrillar A beta1-42 treatment prevents neuronal viability loss. These protective effects were still evident when Cy-3G treatment was initiated after the appearance of oligomeric A beta1-42 neurotoxicity. Taken together, these results suggest that Cy-3G may protect and rescue the neuronal cells from toxicity induced by A beta1-42.
Tarozzi
Andrea
A
Department of Pharmacology, Alma Mater Studiorum, University of Bologna, Bologna, Italy. andrea.tarozzi@unibo.it
Merlicco
Adriana
A
Morroni
Fabiana
F
Franco
Francesca
F
Cantelli-Forti
Giorgio
G
Teti
Gabriella
G
Falconi
Mirella
M
Hrelia
Patrizia
P
D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000633:toxicity*; D000872:Anthocyanins / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D052160:Benzothiazoles; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence / Q000379:methods; D009474:Neurons / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology; D010446:Peptide Fragments / Q000633:toxicity; D013844:Thiazoles / Q000737:chemistry
D016229:Amyloid beta-Peptides; D000872:Anthocyanins; D052160:Benzothiazoles; D005960:Glucosides; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D013844:Thiazoles; C462279:cyanidin-3-O-beta-glucopyranoside; C009462:thioflavin T