Isolation and molecular detection of Ehrlichia from vertebrate animals.
Human monocytic ehrlichiosis (HME), caused by Ehrlichia chaffeensis, was first recognized in 1986. Infection with this pathogen can be fatal in immune compromised and elderly humans. E. chaffeensis can also infect dogs and several wild animals. The clinical symptoms of HME include fever, headache, malaise, myalgia, confusion, rash, lymphadenopathy, and nausea. White-tailed deer serve as the major reservoir host for the natural maintenance of E. chaffeensis. E. canis is primarily responsible for the canine monocytic ehrlichiosis and is endemic throughout the world. It has a significant impact on the health of dogs. The isolation and growth of Ehrlichia species from vertebrate host samples is difficult and time consuming. In this unit, methods to recover E. chaffeensis and E. canis from infected blood samples collected from dogs, deer, and human patients are described. PCR and RT-PCR methods for sensitive detection of Ehrlichia infection are also discussed.
Cheng
Chuanmin
C
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.
Sirigireddy
Kamesh R
KR
Ganta
Roman R
RR
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology; D003670:Deer; D004285:Dogs; D004536:Ehrlichia / Q000235:genetics / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000382:microbiology* / Q000662:veterinary*; D006801:Humans; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification
D012329:RNA, Bacterial
J Clin Microbiol. 1997 Jul;35(7):1681-6
9196173
J Clin Microbiol. 1998 Sep;36(9):2645-51
9705408
Clin Microbiol Rev. 2003 Jan;16(1):37-64
12525424
Am J Vet Res. 1996 Aug;57(8):1175-9
8836370
J Infect Dis. 1974 Oct;130(4):357-67
4140878
J Clin Microbiol. 1997 Oct;35(10):2496-502
9316896
Cell. 1986 Jan 17;44(1):107-15
2866848
Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):2145-2165
11760958
J Clin Microbiol. 2001 Feb;39(2):460-3
11158090
Am J Vet Res. 1980 Aug;41(8):1266-71
7447122
Infect Immun. 1998 Aug;66(8):3892-9
9673277
J Mol Diagn. 2005 May;7(2):308-16
15858156
J Clin Microbiol. 1994 Mar;32(3):589-95
8195363
Microbes Infect. 1999 Apr;1(5):367-76
10602669
Infect Immun. 2003 Jan;71(1):187-95
12496165
J Clin Microbiol. 1994 Nov;32(11):2725-8
7852563
Vet Parasitol. 1998 Nov 27;79(4):325-39
9831955
Curr Protoc Mol Biol. 2001 May;Chapter 24:Unit 24.2
18265209
Infect Immun. 2007 Jan;75(1):135-45
17060466
J Exp Med. 1996 Jan 1;183(1):271-5
8551231
J Infect Dis. 1991 Mar;163(3):564-7
1995730
J Clin Microbiol. 1991 Dec;29(12):2741-5
1757543
Infect Immun. 2002 Mar;70(3):1230-4
11854205
Clin Infect Dis. 2007 Jul 15;45 Suppl 1:S45-51
17582569
N Engl J Med. 1993 Oct 14;329(16):1164-7
8377780
J Wildl Dis. 1997 Jul;33(3):466-73
9249691
Infect Immun. 2002 Jan;70(1):380-8
11748204
Infect Immun. 2004 Jan;72(1):159-67
14688093