title: Isolation and characterization of phenolic compounds from the leaves of Salix matsudana.
abstract: A bioassay-guided in vitro screen has revealed that a 70% methanol extract of the leaves of Salix matsudana shows considerable inhibitory activity against cyclooxygenases (COX-1 and COX-2). A subsequent phytochemical study led to the isolation of a new flavonoid, matsudone A (1), together with five known flavonoids--luteolin (2), isoquercitrin (3), 7-methoxyflavone (4), luteolin 7-O-glucoside (5), 4',7-dihydroxyflavone (6)--and two phenolic glycosides, leonuriside A (7) and piceoside (8). Their structures were elucidated on the basis of extensive 1D- and 2D-NMR studies, high resolution ESI mass spectroscopic analyses and comparisons with literature data. The isolated compounds 1-8 were tested for their inhibitory activities against COX-1 and COX-2. Compounds 1, 5 and 6 were found to have potent inhibitory effect on COX-2 and compounds 3-5 exhibited moderate inhibition against COX-1.
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, P.R. China. lixiang@bjmu.edu
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Xin-feng
authors.initials: XF
authors.lastname: Zhang
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Wang
authors.forename: Yi-nan
authors.initials: YN
authors.lastname: Zheng
authors.forename: Chang-chun
authors.initials: CC
authors.lastname: Yuan
authors.forename: Guang-zhi
authors.initials: GZ
authors.lastname: Sun
mesh_terms: D051545:Cyclooxygenase 1 / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000187:drug effects; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D032108:Salix / Q000737:chemistry*
chemical_list: D016861:Cyclooxygenase Inhibitors; D005419:Flavonoids; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2
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