title: Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes.
abstract: Two proteins, YL41 and YL43, were isolated from 80S ribosomes of Saccharomyces cerevisiae by filtration through a Sephacryl S-200 column and by chromatography on a column of carboxymethylcellulose. Their amino acid compositions are presented. Twenty-four proteins including these two proteins were subjected to sequence analyses by automated Edman degradation. Amino-terminal amino acid sequences were determined for 17 proteins,YS3, YS9, YS23, YS24, YS29, YL6, YL8, YLll, YLI5,YL17, YL23, YL28, YL33, YL37, YL39, YL41, and YL43.YL41, which has a 72.7% lysine and arginine content, was found to be particular to eukaryotic ribosomes. The amino-termini of another seven proteins, YS2, YS5, YS8, YS12,YS13, YS20, and YS27, were suggested to be blocked. Comparison of the amino-terminal sequences with all other ribosomal protein sequences so far available indicates that YS9 shows sequence homology to rat liver ribosomal protein S8 (Wittmann-Liebold et al. 1979).
authors.affiliation: Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University, 2-3, Kasumi 1 Chome, Minamiku, Hiroshima, 734 Japan.
authors.forename: E
authors.initials: E
authors.lastname: Otaka
authors.forename: K
authors.initials: K
authors.lastname: Higo
authors.forename: T
authors.initials: T
authors.lastname: Itoh
mesh_terms: D000180:Acrylic Resins; D000595:Amino Acid Sequence; D000818:Animals; D002852:Chromatography, Ion Exchange; D008969:Molecular Sequence Data; D051381:Rats; D012269:Ribosomal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid
chemical_list: D000180:Acrylic Resins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C023049:sephacryl S 200
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