Separation and characterization of deoxyribonucleases from hepatopancreas of freshwater snail in normality and under in vivo model intoxication.
Deoxyribonucleases (DNases) differing in the subcellular localization and specificity towards native and denatured substrates and in products of cleavage of endogenous DNA were isolated for the first time from the hepatopancreas of freshwater snail by differential sedimentation and preparative isoelectrofocusing. It was defined found that treatment with phenol leads to activation of most of the investigated DNases, especially in lysosomes, and induces two new DNases of lysosomal origin. The possible different participation of certain DNases of the snail hepatopancreas in the regulation of DNA degradation under intoxication is discussed.
Popov
A P
AP
Moscow State Regional University, Moscow, Russia. alexpopoff@rambler.ru
Tsvetkov
I L
IL
Konichev
A S
AS
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000633:toxicity; D002458:Cell Fractionation; D003851:Deoxyribonucleases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D005618:Fresh Water; D043143:Hepatopancreas / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D019800:Phenol / Q000633:toxicity; D012908:Snails / Q000033:anatomy & histology* / Q000187:drug effects / Q000201:enzymology*
D000891:Anti-Infective Agents, Local; D019800:Phenol; D003851:Deoxyribonucleases