In vitro invasion assays: phagocytosis of the extracellular matrix.
Degradation of the extracellular matrix is an essential component of phagocytosis by tumor cells and can be correlated with their invasive capacity. This unit presents flow cytometry based assays for rapid quantitative assessment of both proteolysis and internalization or internalization alone. These assays provide high-throughput, low-cost methods to compare cell lines and test the efficacy of treatments designed to stimulate or inhibit invasion.
Bowden
E T
ET
Georgetown University Medical Center, Washington, DC, USA.
Mueller
S
S
Coopman
P J
PJ
D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002469:Cell Separation; D005109:Extracellular Matrix / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000378:metabolism*; D010587:Phagocytosis