title: [Contents of furanocoumarins in grapefruit juice and health foods].
abstract: Interactions between grapefruit juice and medications have long been recognized. In recent years, several furanocoumarins (FCs) that inhibit P450 activity in intestinal microsomes have been isolated from grapefruit juice. FCs, i.e., bergamottin (BG), bergapten (BP), bergaptol (BT) and 6',7'-dihydroxybergamottin (DHB), in samples was extracted with acetonitrile, and separated on a Phenyl column using 0.1% phosphoric acid-acetonitrile gradient as a mobile phase, with monitoring at 311 nm. The recoveries of BG, BP, BT and DHB from lemon juice spiked at the level of 5.0 microg/g were 103+/-0.7%, 99.5+/-0.2%, 96.5+/-0.2% and 90.1+/-0.2%, respectively. The quantification limits were 1.0 microg/g in samples. The contents of BG, BP and DHB in grapefruit juice (n=13), citrus fruit of 20 species and health food (n=16) were measured. The contents of BG were 0-16 microg/g, 0-16 microg/g and 0-5.6 microg/g, BT were 0-39 microg/g, 0-13 microg/g and 0-28 microg/g, DHB were 0-10 microg/g, 0-35 microg/g and 0-6.2 microg/g, respectively. BP was not detected. These results suggest that patients prescribed calcium antagonists or antiallergic agents should be cautions about their intake of FCs from grapefruit juice, citrus and health foods.
authors.affiliation: Tama Branch Institute, Tokyo Metropolitan Institute of Public Health. Toyko, Japan.
authors.forename: Narue
authors.initials: N
authors.lastname: Sakamaki
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Nakazato
authors.forename: Hiroko
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Kayo
authors.initials: K
authors.lastname: Hagino
authors.forename: Keiko
authors.initials: K
authors.lastname: Hirata
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ushiyama
mesh_terms: D000078223:5-Methoxypsoralen; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry; D032083:Citrus paradisi / Q000737:chemistry*; D004347:Drug Interactions; D058870:Food, Organic / Q000032:analysis*; D011564:Furocoumarins / Q000032:analysis*; D008730:Methoxsalen / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D011564:Furocoumarins; D000078223:5-Methoxypsoralen; C068337:bergamottin; C080771:bergaptol; D008730:Methoxsalen