title: Simple and safe method for simultaneous isolation of microbial RNA and DNA from problematic populations.
abstract: We describe a novel, rapid, and safe method for extracting RNA and DNA from refractory microbes, which avoids the use of phenol or chloroform. It has been used successfully to isolate high-quality nucleic acids from pure cultures and environmental populations known to resist widely used extraction protocols.
authors.affiliation: Biotechnology Research Centre, La Trobe University, Bendigo, Victoria, Australia.
authors.forename: Simon
authors.initials: S
authors.lastname: McIlroy
authors.forename: Kate
authors.initials: K
authors.lastname: Porter
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Seviour
authors.forename: Daniel
authors.initials: D
authors.lastname: Tillett
mesh_terms: D004269:DNA, Bacterial / Q000302:isolation & purification*; D004783:Environmental Microbiology; D008967:Molecular Biology / Q000379:methods*; D012329:RNA, Bacterial / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial
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