title: Chromosomal alterations detected by fluorescence in situ hybridization in urothelial carcinoma and rarer histologic variants of bladder cancer.
abstract: Fluorescence in situ hybridization (FISH) with the UroVysion probe set (Abbott Molecular, Des Plaines, IL) was used to assess 31 bladder cancers for chromosomal abnormalities, including 4 adenocarcinomas, 5 urachal adenocarcinomas, 6 small cell carcinomas, 7 squamous cell carcinomas, and 9 typical urothelial carcinomas. FISH was also used to assess the benign urothelium in 4 cases. There was a significant increase (P < .001) in the mean number of chromosome 3 (2.64 vs 1.51), chromosome 7 (2.61 vs 1.48), and chromosome 17 (2.41 vs 1.41) centromeric signals observed in cells from patients with cancer compared with patients without cancer. Of the 31 tumors, 29 (94%) demonstrated polysomic signal patterns in more than 10% of cells. In the 2 remaining tumor specimens, there was a high percentage of cells (>75%) demonstrating homozygous 9p21 deletion. The data from this study suggest that chromosomal abnormalities detectable by FISH in urothelial carcinoma are also common in rarer histologic variants of bladder cancer.
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: Kipp
authors.forename: Harmony L
authors.initials: HL
authors.lastname: Tyner
authors.forename: Michael B
authors.initials: MB
authors.lastname: Campion
authors.forename: Jesse S
authors.initials: JS
authors.lastname: Voss
authors.forename: R Jeffrey
authors.initials: RJ
authors.lastname: Karnes
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Sebo
authors.forename: Kevin C
authors.initials: KC
authors.lastname: Halling
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*