title: Involvement of glyceraldehyde-3-phosphate dehydrogenase in the X-Ray resistance of HeLa cells.
abstract: We investigated changes in the sub-cellular distribution of glycelaldehyde-3-phosphate dehydrogenase (GAPDH) after X-ray irradiation in HeLa cells. Twenty-four h after irradiation at 5 Gy, nuclear GAPDH levels increased 2.6-fold, whereas total GAPDH levels increased only 1.2-fold. Knockdown of GAPDH using specific small interfering RNA (siRNA) led to sensitization to X-ray-induced cell death. These results suggest that GAPDH plays a role in the radioresponse.
authors.affiliation: Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, Chiba, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Sa
authors.initials: S
authors.lastname: Lu
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D002470:Cell Survival / Q000528:radiation effects; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D034741:RNA, Small Interfering / Q000494:pharmacology; D011836:Radiation Tolerance / Q000187:drug effects / Q000235:genetics*; D014162:Transfection; D014965:X-Rays
chemical_list: D034741:RNA, Small Interfering; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases