title: Cardiac isoenzymes in healthy Holstein calves and calves with experimentally induced endotoxemia.
abstract: This paper describes a controlled study designed to establish normal values for cardiac troponins I and T (cTnI and cTnT) and CK-MB mass in healthy newborn Holstein calves, and to compare values for cTnI, cTnT, CK-MB and total creatine kinase (CK) with age-matched calves experiencing experimentally induced endotoxemia. Nineteen healthy Holstein bull calves, 48 to 72 h of age were used. Baseline cTnI, cTnT, CK-MB and total CK measurements were obtained from control (n = 9) and experimental (n = 10) calves. Controls then received physiological saline and experimental calves received endotoxin (O55:B5 Escherichia coli LPS) intravenously after which cardiac biomarkers and total CK were measured at 3 h, 6 h, 12 h, and 24 h post-initiation of infusion. Measured values were analyzed and compared using analysis of variance (ANOVA) by repeated measure design, with statistical significance set at P < 0.05. The cardiac biomarker cTnT was not detected in any calf at any time point, and CK-MB was only detected in 5 of 95 samples. The cTnI was significantly increased compared to baseline and controls, 3 h post lipopolysaccharide (LPS) infusion. Total CK was significantly increased in LPS administered calves at 18 and 24 h post infusion. The mean, standard deviation, and range for cTnI in healthy controls were 0.023 ng/mL (s = 0.01), and 0.01 to 0.05 ng/mL, respectively. In conclusion, LPS administration was associated with rapid and significant increases in cTnI but CK-MB and cTnT were not detected in the plasma of healthy calves. Total CK values increased significantly following LPS administration. Biochemical evidence of myocardial injury occurs within 3 h following LPS administration to neonatal Holstein calves.
authors.affiliation: Large Animal Internal Medicine, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin 53706, USA. peeks@svm.vetmed.wisc.edu
authors.forename: Simon F
authors.initials: SF
authors.lastname: Peek
authors.forename: Fred S
authors.initials: FS
authors.lastname: Apple
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Murakami
authors.forename: Peter M
authors.initials: PM
authors.lastname: Crump
authors.forename: Susan D
authors.initials: SD
authors.lastname: Semrad
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000201:enzymology; D003402:Creatine Kinase / Q000097:blood*; D052279:Creatine Kinase, MB Form / Q000097:blood; D019446:Endotoxemia / Q000097:blood / Q000201:enzymology / Q000662:veterinary*; D007527:Isoenzymes / Q000097:blood; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009203:Myocardial Infarction / Q000097:blood / Q000662:veterinary; D011897:Random Allocation; D012016:Reference Values; D019210:Troponin I / Q000097:blood*; D020107:Troponin T / Q000097:blood*
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D008070:Lipopolysaccharides; D019210:Troponin I; D020107:Troponin T; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form
references.citation: Am J Physiol. 1988 Feb;254(2 Pt 2):R310-9
references.pmid: 2964208
references.citation: Am J Vet Res. 2002 Feb;63(2):247-50
references.pmid: 11843125
references.citation: Arch Pathol Lab Med. 2004 Apr;128(4):430-4
references.pmid: 15043464
references.citation: Intensive Care Med. 2003 Sep;29(9):1598-600
references.pmid: 12879237
references.citation: Clin Chem. 1997 Dec;43(12):2333-8
references.pmid: 9439451
references.citation: Vet J. 1997 May;153(3):321-7
references.pmid: 9232121
references.citation: Cornell Vet. 1991 Apr;81(2):129-35
references.pmid: 2029837
references.citation: Can J Vet Res. 1995 Jan;59(1):34-9
references.pmid: 7704840
references.citation: Can J Comp Med. 1975 Jan;39(1):1-6
references.pmid: 1089461
references.citation: Vet Rec. 2005 May 28;156(22):714-5
references.pmid: 15923556
references.citation: Clin Chem. 2003 Aug;49(8):1331-6
references.pmid: 12881449
references.citation: J Am Coll Cardiol. 2000 Sep;36(3):970-1062
references.pmid: 10987629
references.citation: CMAJ. 2005 Nov 8;173(10):1191-202
references.pmid: 16275971
references.citation: Am J Vet Res. 1985 Mar;46(3):711-8
references.pmid: 3922266
references.citation: Am J Vet Res. 1994 Feb;55(2):278-87
references.pmid: 8172420
references.citation: Shock. 1999 Nov;12(5):391-401
references.pmid: 10565614
references.citation: J Vet Intern Med. 1996 Jul-Aug;10(4):252-7
references.pmid: 8819051
references.citation: Int J Cardiol. 2004 May;95(1):13-7
references.pmid: 15159032
references.citation: Clin Chem. 1996 Nov;42(11):1770-6
references.pmid: 8906075
references.citation: J Thromb Haemost. 2005 Mar;3(3):508-13
references.pmid: 15748241
references.citation: Vet Clin North Am Food Anim Pract. 2005 Nov;21(3):595-614, v
references.pmid: 16243625
references.citation: Intensive Care Med. 2001 Jun;27(6):965-9
references.pmid: 11497154
references.citation: J Vet Intern Med. 2006 Mar-Apr;20(2):277-83
references.pmid: 16594583
references.citation: Lancet. 2005 Jan 1-7;365(9453):63-78
references.pmid: 15639681
references.citation: Am J Vet Res. 1993 Dec;54(12):2121-7
references.pmid: 8116949
references.citation: Pediatr Crit Care Med. 2004 Nov;5(6):533-8
references.pmid: 15530188
references.citation: Ann Emerg Med. 2004 Jul;44(1):12-9
references.pmid: 15226704
references.citation: Cornell Vet. 1975 Oct;65(4):457-75
references.pmid: 1104259
references.citation: Crit Care Med. 2000 Aug;28(8):2979-83
references.pmid: 10966282
references.citation: Res Vet Sci. 1998 Sep-Oct;65(2):135-7
references.pmid: 9839891