title: Synthesis and biochemical studies of tetraphosphate 5' mRNA cap analogs bearing bisphosphonate modification.
abstract: Dinucleotide cap analogs containing tetraphosphate bridge modified by replacing one of the bridging oxygen atoms with methylene group were synthesized. The analogs have been examined for their stability towards enzymatic hydrolysis by human DcpS and binding affinity for eIF4E. Their inhibitory properties in rabbit reticulocyte lysate (RRL) translational system were studied.
authors.affiliation: Division of Biophysics, Institute of Experimental Physics, University of Warsaw, 02-089 Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Rydzik
authors.forename: Joanna
authors.initials: J
authors.lastname: Zuberek
authors.forename: Joanna
authors.initials: J
authors.lastname: Kowalska
authors.forename: Maciej
authors.initials: M
authors.lastname: Lukaszewicz
authors.forename: Edward
authors.initials: E
authors.lastname: Darzynkiewicz
authors.forename: Jacek
authors.initials: J
authors.lastname: Jemielity
mesh_terms: D000818:Animals; D004164:Diphosphonates / Q000737:chemistry*; D004722:Endoribonucleases / Q000378:metabolism; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D006801:Humans; D014176:Protein Biosynthesis; D012314:RNA Cap Analogs / Q000138:chemical synthesis / Q000737:chemistry*; D011817:Rabbits
chemical_list: D004164:Diphosphonates; D039561:Eukaryotic Initiation Factor-4E; D012314:RNA Cap Analogs; D004722:Endoribonucleases; C485332:DcpS protein, human