[Ulinastatin's early effects on kidney's expression of HSP70 and IL-6 after mechanical injury of kidney].
OBJECTIVE
To investigate the significance of ulinastatin's effects on kidney's expression of IL-6, HSP70 after mechanical injury.


METHODS
Sixity-six adult female Wistar rats were randomly divided into three groups: 6 in the group of control, 30 in the group of trauma, 30 in the group injected ulinastatin after trauma. The model of trauma rats was made with free-fall bio-impactor striking the spinal column and ribs of rats. To detect the IL-6 and HSP70 expression of each group's renal tissue at 1 h, 6 h, 12 h, 24 h and 36 h after trauma with tissue chip and immunohistochemical stain.


RESULTS
IL-6 and HSP70 expression of renal tissue was low with group of control. Compared with group of control, the expression of IL-6 strengthened at 1 h, 6 h, 12 h, 24 h after trauma (P < 0.05),the expression of HSP70 with group of trauma strengthened at 6 h after trauma (P < 0. 05). The expression of IL-6 with group injected ulinastatin after trauma were more lower than group of trauma, the expression of HSP70 at 1 h, 24 h, 36 h after trauma was higher than group of trauma especially at 1 h (P< 0.05).


CONCLUSIONS
Ulinastatin can reduce the risk of renal injury in early times after trauma especially at 1 h. The protective effects of ulinastatin may be associated with the restrict the expression of IL-6, up-regulation of HSP70 expression, and change the proportion between IL-6 and HSP70 expression.
Mao
Wei-dong
WD
Institute of Pathology, The First Hospital of Lanzhou University, Lanzhou 730000, China.
Sun
Shao-hua
SH
D000818:Animals; D005260:Female; D006023:Glycoproteins / Q000494:pharmacology*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D007150:Immunohistochemistry; D015848:Interleukin-5 / Q000096:biosynthesis*; D007668:Kidney / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D006023:Glycoproteins; D018840:HSP70 Heat-Shock Proteins; D015848:Interleukin-5; C028665:urinastatin