Flow cytometers for characterization of microorganisms.
The measurement of microbes is an increasingly important application in flow cytometry. The small size of these organisms can create special difficulties for instruments designed to analyze much larger eukaryotic cells. This unit describes the problems encountered in making microbiological measurements, the instrument requirements, and some approaches that can be taken to optimize the instrument. This material is essential background for the protocols found in chapter 11, Microbiological Applications.
Steen
H B
HB
Norsk Hydro Institute for Cancer Research, Oslo, Norway.
D001419:Bacteria / Q000166:cytology*; D002453:Cell Cycle; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes; D007834:Lasers; D008027:Light; D015233:Models, Statistical; D015203:Reproducibility of Results; D012542:Scattering, Radiation
D005456:Fluorescent Dyes