title: [Genetic effects of MC4R and POU1F1 gene on the growth performance in Jinghai yellow chicken].
abstract: Melanocortin-4 receptor (MC4R) gene and pituitary specific transcription factor 1 (POU1F1) gene were studied as candidate genes on the Jinghai yellow chicken in this study. Polymorphisms of MC4R and POU1F1 gene had been analyzed by PCR-SSCP and DNA sequencing method. The relationship between two candidate genes and the growth performance of Jinghai yellow chicken had also been analyzed by GLM model. The frequency of A/B alleles for MC4R gene in the population was 0.929 and 0.071, and that of C/D alleles for POU1F1 gene was 0.500 and 0.500. The results showed that AA genotype of MC4R gene had higher bodyweight at 4, 8, 12 weeks compared to AB and BB genotype (P<0.05); CD genotype of POU1F1 gene had significantly higher bodyweight at all weeks compared to CC and DD genotype (P<0.01). So, it could be preliminarily deduced that MC4R and POU1F1 gene were probably the major gene on growth performance or a QTL linked gene which associated with growth performance in chicken.
authors.affiliation: Animal Science and Technology Department of Yangzhou University, Yangzhou 225009, China. tyrsm1975tg@126.com
authors.forename: Yong
authors.initials: Y
authors.lastname: Tao
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Li
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Hu
authors.forename: M Dafalla
authors.initials: MD
authors.lastname: Mekki
authors.forename: Kuan-Wei
authors.initials: KW
authors.lastname: Chen
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Wang
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics; D002645:Chickens; D005838:Genotype; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics*; D050817:Transcription Factor Pit-1 / Q000235:genetics*
chemical_list: D044105:Receptor, Melanocortin, Type 4; D050817:Transcription Factor Pit-1