Comparison of five culture methods for Salmonella isolation from swine fecal samples of known infection status.
The current study was conducted to evaluate 5 bacteriologic culture methods (methods 1, 2, 3, 4, and 5) for recovery of Salmonella enterica from swine feces, both for sensitivity of detection (ability to recover Salmonella from a positive sample) and for specificity (not to inadvertently identify an organism as a Salmonella species in a negative sample). Fifty-six negative samples and 46 positive samples were processed using each of the 5 methods, which differed primarily in the combinations of enrichment media used. All negative samples were negative for Salmonella when cultured by all 5 methods (100% specificity). Two of the methods (methods 1 and 4) resulted in the recovery of significantly less (P < 0.05) Salmonella when compared with the remaining 3 methods (methods 2, 3, and 5). No one method was successful in recovering Salmonella from all positive samples, although recovery with method 2 was statistically similar to the total number of positive samples analyzed (42 vs. 46 Salmonella-positive samples, P > 0.05). This study shows that culture methods differ significantly in their performance regarding the isolation of Salmonella from swine fecal samples.
Love
Brenda C
BC
Department of Veterinary Biosciences, Oklahoma Animal Disease Diagnostic Laboratory, Oklahoma State University, Stillwater, OK 74078, USA. brenda.love@okstate.edu
Rostagno
Marcos H
MH
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D005243:Feces / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000175:diagnosis* / Q000382:microbiology; D019779:Salmonella enterica / Q000254:growth & development / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*