[Somatic cell nuclear transfer and centrosome inheritance].
The developmental competence of embryos cloned from somatic cells depends on the cellular event and molecular process, such as separation of chromosomes and reorganization of spindle after nuclear transfer. Centrosome, the main microtubule organizing centers in a cell, is crucial for reorganization of spindle and normal separation of chromosomes during mitosis. Aberrant of centrosomes will lead to aneuploidy of blastomere and developmental failure of embryo. This paper expounded the situation of animal somatic cell nuclear transfer (SCNT) and biological functions of centrosome and analyzed the inheritance mechanism of centrosome during gametogenesis and fertilization. Additionally, the study condition of centrosome and its associated proteins in SCNT embryos were introduced, which provided a new clue to study the de-velopmental abnormality of cloned embryos and animals.
Du
Wei-Hua
WH
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China dwh679@sohu.com
Zhu
Hua-Bin
HB
Hao
Hai-Sheng
HS
Wang
Dong
D
D000818:Animals; D018385:Centrosome / Q000502:physiology*; D047108:Embryonic Development / Q000235:genetics*; D006801:Humans; D008938:Mitosis / Q000235:genetics; D053652:Nuclear Transfer Techniques