title: The very-long-chain hydroxy fatty acyl-CoA dehydratase PASTICCINO2 is essential and limiting for plant development.
abstract: Very-long-chain fatty acids (VLCFAs) are synthesized as acyl-CoAs by the endoplasmic reticulum-localized elongase multiprotein complex. Two Arabidopsis genes are putative homologues of the recently identified yeast 3-hydroxy-acyl-CoA dehydratase (PHS1), the third enzyme of the elongase complex. We showed that Arabidopsis PASTICCINO2 (PAS2) was able to restore phs1 cytokinesis defects and sphingolipid long chain base overaccumulation. Conversely, the expression of PHS1 was able to complement the developmental defects and the accumulation of long chain bases of the pas2-1 mutant. The pas2-1 mutant was characterized by a general reduction of VLCFA pools in seed storage triacylglycerols, cuticular waxes, and complex sphingolipids. Most strikingly, the defective elongation cycle resulted in the accumulation of 3-hydroxy-acyl-CoA intermediates, indicating premature termination of fatty acid elongation and confirming the role of PAS2 in this process. We demonstrated by in vivo bimolecular fluorescence complementation that PAS2 was specifically associated in the endoplasmic reticulum with the enoyl-CoA reductase CER10, the fourth enzyme of the elongase complex. Finally, complete loss of PAS2 function is embryo lethal, and the ectopic expression of PHS1 led to enhanced levels of VLCFAs associated with severe developmental defects. Altogether these results demonstrate that the plant 3-hydroxy-acyl-CoA dehydratase PASTICCINO2 is an essential and limiting enzyme in VLCFA synthesis but also that PAS2-derived VLCFA homeostasis is required for specific developmental processes.
authors.affiliation: Laboratoire Biologie Cellulaire, Institut National de la Recherche Agronomique, 78000 Versailles Cedex, France.
authors.forename: Liên
authors.initials: L
authors.lastname: Bach
authors.forename: Louise V
authors.initials: LV
authors.lastname: Michaelson
authors.forename: Richard
authors.initials: R
authors.lastname: Haslam
authors.forename: Yannick
authors.initials: Y
authors.lastname: Bellec
authors.forename: Lionel
authors.initials: L
authors.lastname: Gissot
authors.forename: Jessica
authors.initials: J
authors.lastname: Marion
authors.forename: Marco
authors.initials: M
authors.lastname: Da Costa
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Boutin
authors.forename: Martine
authors.initials: M
authors.lastname: Miquel
authors.forename: Frédérique
authors.initials: F
authors.lastname: Tellier
authors.forename: Frederic
authors.initials: F
authors.lastname: Domergue
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Markham
authors.forename: Frederic
authors.initials: F
authors.lastname: Beaudoin
authors.forename: Johnathan A
authors.initials: JA
authors.lastname: Napier
authors.forename: Jean-Denis
authors.initials: JD
authors.lastname: Faure
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D006836:Hydro-Lyases / Q000235:genetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D012639:Seeds / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D029681:Arabidopsis Proteins; D005227:Fatty Acids; C477363:PEPINO protein, Arabidopsis; D029701:Saccharomyces cerevisiae Proteins; D006836:Hydro-Lyases; C529568:Phs1 protein, S cerevisiae
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