title: Comparative immunohistochemistry of L19 and F16 in non-small cell lung cancer and mesothelioma: two human antibodies investigated in clinical trials in patients with cancer.
abstract: The antibody-mediated targeted delivery of therapeutics to tumor sites is an attractive avenue for combating cancer while sparing normal tissues. Indeed, five derivatives of the human monoclonal antibodies L19 and F16, specific to splice isoforms of fibronectin and tenascin-C, are currently being investigated in clinical trials in patients with malignancies. Until now, a comparative immunohistochemical analysis of these antibodies, which recognize components of the modified extracellular matrix, was missing. Here, we report that the majority of NSCLC and mesothelioma specimens are stained with both antibodies in the stroma, while non-tumoral lung and mesothelium samples rarely exhibit reactivity with either L19 or F16. In our analysis, the anti-tenascin F16 antibody was found to generally exhibit a stronger staining of desmoplastic stroma surrounding tumor. This superior performance was found to be particularly striking in the case of low-grade non-small cell lung cancer.
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zurich, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.
authors.forename: Marta
authors.initials: M
authors.lastname: Pedretti
authors.forename: Alex
authors.initials: A
authors.lastname: Soltermann
authors.forename: Stephan
authors.initials: S
authors.lastname: Arni
authors.forename: Walter
authors.initials: W
authors.lastname: Weder
authors.forename: Dario
authors.initials: D
authors.lastname: Neri
authors.forename: Sven
authors.initials: S
authors.lastname: Hillinger
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000556:secondary / Q000628:therapy; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology / Q000556:secondary / Q000628:therapy*; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000556:secondary / Q000628:therapy; D016022:Case-Control Studies; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D005353:Fibronectins / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D008297:Male; D008654:Mesothelioma / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D009367:Neoplasm Staging; D011379:Prognosis; D055752:Small Cell Lung Carcinoma / Q000276:immunology / Q000556:secondary / Q000628:therapy; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D019063:Tenascin / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D005353:Fibronectins; D019063:Tenascin