title: [Transmission and molecular characteristics of carbapenem-resistant Acinetobacter baumannii].
abstract: OBJECTIVE
  To study the mode of transmission and molecular characteristics on carbapenem-resistant Acinetobacter baumannii strain. Strains were isolated from different parts of samples in various patients.
  
  
  METHODS
  Clinical information of carbapenem-resistant Acinetobacter baumannii isolates were stored and analyzed by WHONET 5.4 software. The transmission and pathopoiesis of the strains were learned through case file review. Genotypes of isolates were identified by pulse-field gel electrophoresis (PFGE) and genes of carbapenemase were detected by multiple PCR, in order to find molecular characteristics and relatedness between strains.
  
  
  RESULTS
  29 stains of Acinetobacter baumannii resistant to carbapenem were isolated from 2 or more kinds of samples among 13 patients'. Two genotypes were identified by PFGE: genotype A was obtained from 22 isolates in 11 patients and genotype B was obtained from 7 isolates in 4 patients. PCR amplification showed that all strains possessed OXA-23 gene except 1, and all strains possessed Integrase gene I except 3.
  
  
  CONCLUSIONS
  There were 2 different genotypes from 29 strains of carbapenem-resistant Acinetobacter baumannii with Genotype A as the main type. OXA-23 carbapenemase gene and integrase gene I were detected from most of the isolates. All the strains could be easily transmitted in the body of the patients and among patients, hence becoming the epidemic pathogen of iatrogenic infection.
authors.affiliation: Department of Microbiology, PLA General Hospital, Beijing, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.forename: Ding-Xia
authors.initials: DX
authors.lastname: Shen
authors.forename: Zhong-Qiang
authors.initials: ZQ
authors.lastname: Yan
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Luo
authors.forename: Jing-Rong
authors.initials: JR
authors.lastname: Cao
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology* / Q000635:transmission*; D040981:Acinetobacter baumannii / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology; D003428:Cross Infection / Q000382:microbiology / Q000635:transmission; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans
chemical_list: D015780:Carbapenems