Development of a novel selective detection method for 5-formyl-2'-deoxyuridine by fluorescent derivatization.
5-Formyl-2'-deoxyuridine (fdUrd) is derived from the oxidation of thymidine and is known to induce mutation (A:T to G:C) in DNA. We planned to develop a novel selective detection method for fdUrd in oligodeoxynucleotide (ODN) based on specific fluorescent derivatization of fdUrd using 2-aminothiophenol derivatives (ATs) as fluorogenic reagents. To achieve this goal, we first synthesized 5-(benzothiazol-2-yl)-2'-deoxyuridine derivatives (btdUrds) via the reaction between fdUrd and 5-substituted ATs. We report herein the synthesis and UV absorbance and fluorescence properties of btdUrds in different pH conditions.
Hirose
Wataru
W
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Sato
Kousuke
K
Matsuda
Akira
A
D000814:Aniline Compounds / Q000737:chemistry*; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011741:Pyrimidine Nucleosides / Q000138:chemical synthesis / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D011741:Pyrimidine Nucleosides; C005464:2-aminothiophenol; C061519:5-formyl-2'-deoxyuridine; D003857:Deoxyuridine