title: PtdIns5P regulation through evolution: roles in membrane trafficking?
abstract: Phosphoinositides are lipid second messengers that are essential for many cellular processes, including signal transduction and cell compartmentalization. Among them, phosphatidylinositol 5-phosphate (PtdIns5P) is the least characterized, although several proteins involved in its regulation are implicated in human diseases. We studied the distribution of 32 PtdIns5P-metabolizing proteins in 39 eukaryotic genomes. Phylogenetic profiles identify four groups of co-evolving proteins, confirming known protein complexes and revealing new ones. The complexes comprise a phosphatase, a kinase and a regulator; this indicates that physical interactions between the three partners are necessary for the acute spatial regulation of PtdIns5P turnover. By examining PtdIns5P metabolism in this new perspective, we propose a role for PtdIns5P in membrane trafficking from late endosomal compartments to the plasma membrane.
authors.affiliation: Department of Structural Biology and Genomics, rue Laurent Fries, Illkirch, F-67400 France.
authors.forename: Odile
authors.initials: O
authors.lastname: Lecompte
authors.forename: Olivier
authors.initials: O
authors.lastname: Poch
authors.forename: Jocelyn
authors.initials: J
authors.lastname: Laporte
mesh_terms: D000818:Animals; D019143:Evolution, Molecular; D023281:Genomics; D006801:Humans; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism / Q000502:physiology*; D010716:Phosphatidylinositols / Q000502:physiology; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D010802:Phylogeny; D015290:Second Messenger Systems; D015398:Signal Transduction
chemical_list: D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; C113567:phosphatidylinositol 5-phosphate; D010770:Phosphotransferases