Synthesis and silencing properties of siRNAs possessing lipophilic groups at their 3'-termini.
Short interfering RNAs (siRNAs) conjugated with lipophilic groups at their 3'-termini were synthesized. The silencing activities of these siRNAs were examined by a dual-luciferase assay. It was found that the '-end of the passenger strand (sense strand) was better than the guide strand (antisense strand) for connecting bulky lipophilic molecules, particularly a rigid molecule such as cholesterol.
Ueno
Yoshihito
Y
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501-1193, Japan. uenoy@gifu-u.ac.jp
Kawada
Koshi
K
Shibata
Aya
A
Yoshikawa
Kayo
K
Wataya
Yusuke
Y
Kitade
Yukio
Y
D002784:Cholesterol / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008156:Luciferases / Q000235:genetics; D019301:Oleic Acid / Q000737:chemistry; D019308:Palmitic Acid / Q000737:chemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000737:chemistry*
D008055:Lipids; D034741:RNA, Small Interfering; D019301:Oleic Acid; D019308:Palmitic Acid; D002784:Cholesterol; D008156:Luciferases