Cohesin and recombination proteins influence the G1-to-S transition in azygotic meiosis in Schizosaccharomyces pombe.
To determine whether recombination and/or sister-chromatid cohesion affect the timing of meiotic prophase events, the horsetail stage and S phase were analyzed in Schizosaccharomyces pombe strains carrying mutations in the cohesin genes rec8 or rec11, the linear element gene rec10, the pairing gene meu13, the double-strand-break formation genes rec6, rec7, rec12, rec14, rec15, and mde2, and the recombination gene dmc1. The double-mutant strains rec8 rec11 and rec8 rec12 were also assayed. Most of the single and both double mutants showed advancement of bulk DNA synthesis, start of nuclear movement (horsetail stage), and meiotic divisions by up to 2 hr. Only mde2 and dmc1 deletion strains showed wild-type timing. Contrasting behavior was observed for rec8 deletions (delayed by 1 hr) compared to a rec8 point mutation (advanced by 1 hr). An hypothesis for the role of cohesin and recombination proteins in the control of the G(1)-to-S transition is proposed. Finally, differences between azygotic meiosis and two other types of fission yeast meiosis (zygotic and pat1-114 meiosis) are discussed with respect to possible control steps in meiotic G(1).
Doll
Eveline
E
Institute of Cell Biology, University of Berne, CH-3012 Berne, Switzerland.
Molnar
Monika
M
Cuanoud
Gabriella
G
Octobre
Guillaume
G
Latypov
Vitaly
V
Ludin
Katja
K
Kohli
Jürg
J
D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002842:Chromatids / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D016193:G1 Phase; D005800:Genes, Fungal; D008540:Meiosis / Q000235:genetics*; D011995:Recombination, Genetic; D016196:S Phase; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D015053:Zygote / Q000378:metabolism; D000097722:Cohesins
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D029702:Schizosaccharomyces pombe Proteins; D000097722:Cohesins
Nature. 1998 Apr 23;392(6678):828-31
9572143
Mol Cell Biol. 1999 May;19(5):3515-28
10207075
Mol Gen Genet. 1977 Feb 28;151(1):77-81
865481
Genes Dev. 2000 Nov 1;14(21):2757-70
11069892
Cell. 2006 Apr 7;125(1):59-69
16615890
Curr Genet. 2005 Nov;48(5):323-33
16252089
Curr Genet. 1985;10(4):297-311
3870979
EMBO J. 1994 Apr 15;13(8):1873-80
8168485
Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10952-7
16043696
Science. 1994 Apr 8;264(5156):270-3
8146661
Genetics. 2003 Nov;165(3):1031-43
14668362
Proc Natl Acad Sci U S A. 2005 Apr 19;102(16):5797-801
15805194
Genetics. 1995 Sep;141(1):61-73
8536990
Methods Enzymol. 1991;194:795-823
2005825
J Cell Sci. 2004 Jul 1;117(Pt 15):3343-51
15226405
J Cell Sci. 2003 Jan 15;116(Pt 2):259-71
12482912
Genetics. 2004 Jun;167(2):593-605
15238514
Genetics. 2001 Dec;159(4):1467-78
11779789
Mol Cell Biol. 2005 Aug;25(15):6330-7
16024772
Nature. 2008 Feb 21;451(7181):1018-21
18256600
Microbiol Mol Biol Rev. 1999 Jun;63(2):349-404
10357855
Genes Dev. 2000 Feb 15;14(4):493-503
10691741
Mol Gen Genet. 1985;198(3):416-21
25864229
Nature. 1997 Mar 27;386(6623):414-7
9121560
Genetics. 1999 May;152(1):117-28
10224247
Genetics. 1999 Sep;153(1):57-68
10471700
Nature. 1996 Oct 31;383(6603):840-3
8893012
EMBO J. 2002 Jun 3;21(11):2807-18
12032093
Genetics. 2000 Apr;154(4):1497-508
10747048
Chromosoma. 1995 Nov;104(3):203-14
8529460
Curr Biol. 2005 Sep 20;15(18):1663-9
16169489
Genetics. 2006 Nov;174(3):1105-14
16980386
Cell. 1997 Feb 7;88(3):375-84
9039264
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8395-402
11459981
Experientia. 1994 Mar 15;50(3):295-306
8143803
EMBO J. 1994 Apr 15;13(8):1881-7
8168486
Genes Dev. 2005 Apr 1;19(7):853-62
15805471
Genes Dev. 1994 Jan;8(2):203-10
8299939
Annu Rev Genet. 2001;35:673-745
11700297
Genetics. 1992 Feb;130(2):251-62
1541389
Exp Cell Res. 1983 Apr 15;145(1):209-17
6343102
Nucleic Acids Res. 2000 Jul 15;28(14):2709-16
10908327
Mol Cell. 2005 May 13;18(4):491-8
15893732
J Cell Sci. 1979 Oct;39:215-33
528581
Curr Top Dev Biol. 2004;61:29-60
15350396
Chromosoma. 2008 Oct;117(5):431-44
18449558
Genetics. 1997 Jun;146(2):481-9
9177999
Genetics. 2005 May;170(1):95-106
15744055
Genetics. 1990 Nov;126(3):563-74
2249756
Mol Cell. 2000 May;5(5):883-8
10882124
Nat Genet. 2002 Sep;32(1):143-7
12161753
Genetics. 1997 Aug;146(4):1253-64
9258671
EMBO J. 2001 Jul 16;20(14):3871-81
11447128
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3850-4
333447
Eukaryot Cell. 2004 Jun;3(3):598-609
15189982
J Cell Sci. 2003 May 1;116(Pt 9):1719-31
12665553
Science. 2000 Oct 27;290(5492):806-9
11052944
Nature. 1981 Aug 6;292(5823):558-60
7254352
Science. 2003 Aug 8;301(5634):785-9
12907787
J Biol Chem. 2006 Jan 20;281(3):1338-44
16286472
Genetics. 2001 Feb;157(2):519-32
11156975
J Cell Biol. 1999 Jun 14;145(6):1233-49
10366596
Nature. 2001 Jan 18;409(6818):359-63
11201746
Exp Cell Res. 1974 Sep;88(1):127-34
4472733
Curr Genet. 2004 Jan;44(6):317-28
12955454
Mol Biol Cell. 1998 Oct;9(10):2739-50
9763441
EMBO J. 2004 Apr 21;23(8):1792-803
14988732
J Cell Biol. 1993 Apr;121(2):241-56
8468345
Mol Cell Biol. 2005 Jun;25(11):4377-87
15899844
Philos Trans R Soc Lond B Biol Sci. 2004 Jan 29;359(1441):99-108
15065662
Genetics. 2003 Jul;164(3):881-93
12871901
EMBO J. 2000 Jul 17;19(14):3831-40
10899136
Curr Top Dev Biol. 2001;52:1-53
11529427
J Cell Sci. 2001 Aug;114(Pt 15):2843-53
11683417
Nature. 2006 Jul 6;442(7098):45-50
16823445
Curr Genet. 1995 Apr;27(5):440-6
7586030
Curr Biol. 2005 Nov 22;15(22):2056-62
16303567
EMBO J. 1988 Mar;7(3):761-7
2840284
Nature. 1999 Jul 29;400(6743):461-4
10440376
Genetics. 1989 Sep;123(1):45-54
2806887
Mol Biol Cell. 2002 Jun;13(6):2080-90
12058071
J Cell Biol. 2006 Aug 14;174(4):499-508
16893973
Cell. 1992 May 1;69(3):439-56
1581960
Mol Cell Biol. 1984 Nov;4(11):2529-31
6392855
Genes Dev. 1997 Apr 1;11(7):876-86
9106659
Chromosome Res. 2007;15(5):523-37
17674143
Cell. 1998 Aug 7;94(3):375-86
9708739
Chromosoma. 2006 Aug;115(4):330-40
16532353
Science. 2003 May 16;300(5622):1152-5
12750522