[Effects of glucose and its modulation by insulin on proliferation and differentiation of rat dental follicle cells in vitro].
OBJECTIVE
To investigate the effects of glucose and its modulation by insulin on proliferation and differentiation of rat dental follicle cells (DFCs) in vitro.


METHODS
The rat dental follicle cells from 7-day neonatal rat were cultured. The effects of different doses of glucose concentration (5.5, 16.5, and 49.5mmol/L), alone with insulin(6mug/ml), on rat dental follicle cells was examined in vitro. The DFCs proliferation and alkaline phosphatase (ALP) were examined after 1,3,7,9 days and 1-7 days of culture, respectively. The data were analyzed using SPSS13.0 software package.


RESULTS
At 1,3 day, DFCs proliferation greatly enhanced at 16.5mmol/L glucose, and decreased at 49.5mmol/L glucose. Insulin treatment greatly decreased the proliferation of 16.5mmol/L glucose and increased the proliferation of 49.5mmol/L glucose. With high concentrations of glucose, cell proliferation greatly increased at 7,9 day, insulin treatment greatly increased the proliferation of DFCs. High concentrations of glucose greatly increased the DFCs ALP activity in 1-7 days. Insulin treatment greatly decreased DFCs ALP activity with high concentrations of glucose (P<0.05).


CONCLUSIONS
This study suggests that insulin contains the deleterious effect of acute high concentrations of glucose on rat DFCs proliferation and differentiation.
E
Ling-ling
LL
Research Institute of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China. elingling@sohu.com
Liu
Hong-chen
HC
Xu
Lu-lu
LL
Wang
Dong-sheng
DS
Lv
Jiao
J
D000469:Alkaline Phosphatase; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003795:Dental Sac / Q000502:physiology*; D005947:Glucose / Q000502:physiology*; D066298:In Vitro Techniques; D007328:Insulin; D051381:Rats
D007328:Insulin; D000469:Alkaline Phosphatase; D005947:Glucose