[Genetic polymorphism of 15 STR loci in Tibetan population].
The polymorphism distributions of 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, and FGA) were investigated in a Tibetan population by multiplex PCR amplification using five fluorochromes (6FAM, VIC, NED, PET, LIZ). Gene frequency, discrimination power (DP), heterozygosity (H), polymorphism information content (PIC) and probability of paternity exclusion (EPP) were calculated, and all loci were tested for Hardy-Weinberg equilibrium. Results indicate that the gene frequency of these 15 STR loci is in Hardy-Weinberg equilibrium. The DP is at 0.7555-0.9602, H is at 0.5651-0.8530, PIC is at 0.5528-0.8456, and EPP is at 0.3811-0.8549. Cumulative DP of the 15 STR is 0.99999999, and cumulative EPP is 0.999999997. Therefore, these 15 STR loci can be used as genetic markers of Tibetan populations in anthropological studies, linkage analysis of genetic diseases, individual identification and paternity testing in forensic medicine.
Li
Ning
N
College of Basic Science, Liaoning Medical College, Jinzhou 121001, China. lining197859@yahoo.com.cn
Su
Yu-Hong
YH
Xi
Huan-Jiu
HJ
Ren
Fu
F
Huang
Ke-Qiang
KQ
Xiao
Yan-Jie
YJ
Wen
You-Feng
YF
Li
Chun-Shan
CS
Li
Chang-Yong
CY
Wang
Kun
K
Cheng
Jian-Guo
JG
D005787:Gene Frequency / Q000235:genetics; D008040:Genetic Linkage / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D018609:Tibet