[The influence on the growth of endothelial cells onto titanium-oxide films modified by poly-L-lysine and immobilized with fibronectin].
In this work, a surface biological and chemical modification method was used for improving the biological behavior of endothelial cells onto titanium-oxide films. The titanium-oxide films were first activated by HCl and H2O2 to produce hydroxyl group, then coated with poly-L-lysine and further immobilized with fibronectin. The surface characteristics of samples were analyzed by Fourier Transfer Infrared Spectrum, X-ray Photoelectron Spectroscopy and contact angle method. The biological behavior of cultured human umbilical vein endothelial cell (HUVEC) seeding onto different samples surface was evaluated by the in vitro HUVEC original cultured experiment. The results showed that the method of coating with poly-L-lysine and immobilizing with fibronectin can promote the adhesion and growth of endothelial cells onto titanium-oxide film.
Wang
Lu
L
School of Materials Science and Engineering, Southwest Jiaotong University, Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Chengdu 610031, China.
Chen
Junying
J
Huang
Nan
N
D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology*; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D011107:Polylysine / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D014471:Umbilical Veins / Q000166:cytology
D020099:Coated Materials, Biocompatible; D005353:Fibronectins; C009495:titanium dioxide; D011107:Polylysine; D014025:Titanium