title: Interactions between M protein and other structural proteins of severe, acute respiratory syndrome-associated coronavirus.
abstract: Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) structural proteins (S, E, M, and NC) localize in different subcellular positions when expressed individually. However, SARS-CoV M protein is co-localized almost entirely with S, E, or NC protein when co-expressed in the cells. On the other hand, only partial co-localization was observed when S and E, S and NC, or E and NC were co-expressed in the cells. Interactions between SARS-CoV M and other structural proteins but not interactions between S and E, S and NC, or E and NC were further demonstrated by co-immunoprecipitation assay. These results indicate that SARS-CoV M protein, similar to the M proteins of other coronaviruses, plays a pivotal role in virus assembly. The cytoplasmic C-terminus domain of SARS-CoV M protein was responsible for binding to NC protein. Multiple regions of M protein interacted with E and S proteins. A model for the interactions between SARS-CoV M protein and other structural proteins is proposed. This study helps us better understand protein-protein interactions during viral assembly of SARS-CoV.
authors.affiliation: Graduate Institute of Molecular and Cellular Biology, Tzu Chi University, 701, Section 3, Chung Yang Road, Hualien, Taiwan.
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Hsieh
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Li
authors.forename: Shih-Chi
authors.initials: SC
authors.lastname: Chen
authors.forename: Shih-Yen
authors.initials: SY
authors.lastname: Lo
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D000086862:Coronavirus M Proteins; D017353:Gene Deletion; D011485:Protein Binding; D025941:Protein Interaction Mapping; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000378:metabolism*; D045169:Severe Acute Respiratory Syndrome / Q000378:metabolism / Q000821:virology*; D014709:Vero Cells; D014763:Viral Matrix Proteins / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000378:metabolism*
chemical_list: D000086862:Coronavirus M Proteins; C000706237:M protein, SARS-CoV; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins
references.citation: Int J Biochem Cell Biol. 2006;38(4):589-99
references.pmid: 16343974
references.citation: Lancet Infect Dis. 2004 Nov;4(11):663-71
references.pmid: 15522678
references.citation: J Virol. 1999 Sep;73(9):7441-52
references.pmid: 10438834
references.citation: J Cell Biol. 1995 Oct;131(2):339-49
references.pmid: 7593163
references.citation: J Virol. 1997 Dec;71(12):9278-84
references.pmid: 9371586
references.citation: J Biomed Sci. 2007 Jan;14(1):31-41
references.pmid: 17237979
references.citation: J Gen Virol. 2002 Dec;83(Pt 12):3085-3092
references.pmid: 12466485
references.citation: J Virol. 2005 Nov;79(22):13848-55
references.pmid: 16254320
references.citation: Lancet. 2003 Apr 19;361(9366):1319-25
references.pmid: 12711465
references.citation: Lancet. 2003 Jul 26;362(9380):263-70
references.pmid: 12892955
references.citation: Adv Exp Med Biol. 1984;173:53-64
references.pmid: 6331128
references.citation: J Biomed Sci. 2003 Nov-Dec;10(6 Pt 2):664-75
references.pmid: 14631105
references.citation: Proc Natl Acad Sci U S A. 1986 Nov;83(21):8122-6
references.pmid: 3095828
references.citation: J Exp Med. 2005 Aug 1;202(3):415-24
references.pmid: 16043521
references.citation: J Biochem Mol Biol. 2005 Jul 31;38(4):381-5
references.pmid: 16053703
references.citation: J Virol Methods. 2005 Nov;129(2):152-61
references.pmid: 16023741
references.citation: J Virol. 1998 Aug;72(8):6838-50
references.pmid: 9658133
references.citation: FEBS Lett. 2006 May 29;580(13):3192-200
references.pmid: 16684538
references.citation: J Virol. 2000 Sep;74(17):8127-34
references.pmid: 10933723
references.citation: Biochemistry. 1986 Mar 25;25(6):1335-9
references.pmid: 3008826
references.citation: EMBO J. 1996 Apr 15;15(8):2020-8
references.pmid: 8617249
references.citation: J Virol. 2000 Feb;74(3):1566-71
references.pmid: 10627571
references.citation: Microbes Infect. 2007 Jan;9(1):119-26
references.pmid: 17142081
references.citation: J Gen Virol. 2005 May;86(Pt 5):1423-1434
references.pmid: 15831954
references.citation: Biochem Biophys Res Commun. 2004 Jun 11;318(4):833-8
references.pmid: 15147946
references.citation: J Virol. 2007 Mar;81(5):2418-28
references.pmid: 17166901
references.citation: Science. 2003 May 30;300(5624):1377-8
references.pmid: 12775826
references.citation: J Biomed Sci. 2008 Jul;15(4):417-25
references.pmid: 18398700
references.citation: J Gen Virol. 2003 Sep;84(Pt 9):2305-2315
references.pmid: 12917450
references.citation: J Biomed Sci. 2008 May;15(3):301-10
references.pmid: 18398701
references.citation: Adv Exp Med Biol. 1998;440:361-5
references.pmid: 9782304
references.citation: Virology. 2007 May 10;361(2):304-15
references.pmid: 17210170
references.citation: Bioinformatics. 1998;14(4):378-9
references.pmid: 9632836
references.citation: Science. 2003 May 30;300(5624):1394-9
references.pmid: 12730500
references.citation: Science. 2003 May 30;300(5624):1351
references.pmid: 12775803
references.citation: J Virol. 2004 Nov;78(22):12557-65
references.pmid: 15507643