title: Laboratory maintenance of Mycobacterium tuberculosis.
abstract: This unit includes protocols for the laboratory maintenance of Mycobacterium tuberculosis, including growth on liquid and solid media as well as recommendations for long-term strain storage. Considerations for working with M. tuberculosis at Biosafety Level 3 containment are also discussed.
authors.affiliation: Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Michelle H
authors.initials: MH
authors.lastname: Larsen
authors.forename: Karolin
authors.initials: K
authors.lastname: Biermann
authors.forename: William R
authors.initials: WR
authors.lastname: Jacobs
mesh_terms: D001431:Bacteriological Techniques; D019411:Clinical Laboratory Techniques; D003264:Containment of Biohazards / Q000379:methods; D003470:Culture Media; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000254:growth & development*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D003470:Culture Media