title: Occurrence of autoantibodies to annexin I, 14-3-3 theta and LAMR1 in prediagnostic lung cancer sera.
abstract: OBJECTIVE
  We have implemented a high throughput platform for quantitative analysis of serum autoantibodies, which we have applied to lung cancer for discovery of novel antigens and for validation in prediagnostic sera of autoantibodies to antigens previously defined based on analysis of sera collected at the time of diagnosis.
  
  
  METHODS
  Proteins from human lung adenocarcinoma cell line A549 lysates were subjected to extensive fractionation. The resulting 1,824 fractions were spotted in duplicate on nitrocellulose-coated slides. The microarrays produced were used in a blinded validation study to determine whether annexin I, PGP9.5, and 14-3-3 theta antigens previously found to be targets of autoantibodies in newly diagnosed patients with lung cancer are associated with autoantibodies in sera collected at the presymptomatic stage and to determine whether additional antigens may be identified in prediagnostic sera. Individual sera collected from 85 patients within 1 year before a diagnosis of lung cancer and 85 matched controls from the Carotene and Retinol Efficacy Trial (CARET) cohort were hybridized to individual microarrays.
  
  
  RESULTS
  We present evidence for the occurrence in lung cancer sera of autoantibodies to annexin I, 14-3-3 theta, and a novel lung cancer antigen, LAMR1, which precede onset of symptoms and diagnosis.
  
  
  CONCLUSIONS
  Our findings suggest potential utility of an approach to diagnosis of lung cancer before onset of symptoms that includes screening for autoantibodies to defined antigens.
authors.affiliation: Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. jiqiu@fhcrc.org
authors.forename: Ji
authors.initials: J
authors.lastname: Qiu
authors.forename: Gina
authors.initials: G
authors.lastname: Choi
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Pitteri
authors.forename: Sandra R
authors.initials: SR
authors.lastname: Pereira-Faca
authors.forename: Alexei L
authors.initials: AL
authors.lastname: Krasnoselsky
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Randolph
authors.forename: Gilbert S
authors.initials: GS
authors.lastname: Omenn
authors.forename: Cim
authors.initials: C
authors.lastname: Edelstein
authors.forename: Matt J
authors.initials: MJ
authors.lastname: Barnett
authors.forename: Mark D
authors.initials: MD
authors.lastname: Thornquist
authors.forename: Gary E
authors.initials: GE
authors.lastname: Goodman
authors.forename: Dean E
authors.initials: DE
authors.lastname: Brenner
authors.forename: Ziding
authors.initials: Z
authors.lastname: Feng
authors.forename: Samir M
authors.initials: SM
authors.lastname: Hanash
mesh_terms: D048948:14-3-3 Proteins / Q000276:immunology*; D000230:Adenocarcinoma / Q000175:diagnosis / Q000276:immunology*; D017305:Annexin A1 / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D042241:Early Diagnosis; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000276:immunology*; D011237:Predictive Value of Tests; D040081:Protein Array Analysis; D017474:Receptors, Laminin / Q000276:immunology; D015203:Reproducibility of Results; D012269:Ribosomal Proteins; D043222:Ubiquitin Thiolesterase / Q000276:immunology
chemical_list: D048948:14-3-3 Proteins; D017305:Annexin A1; D001323:Autoantibodies; C510624:RPSA protein, human; D017474:Receptors, Laminin; D012269:Ribosomal Proteins; C482860:UCHL1 protein, human; D043222:Ubiquitin Thiolesterase
references.citation: Cancer. 1988 Jul 15;62(2):283-90
references.pmid: 3289728
references.citation: Nat Immunol. 2002 Nov;3(11):991-8
references.pmid: 12407406
references.citation: J Immunol. 1995 Mar 1;154(5):2266-80
references.pmid: 7868899
references.citation: Clin Cancer Res. 1997 Feb;3(2):227-31
references.pmid: 9815677
references.citation: FEBS Lett. 2005 May 23;579(13):2873-7
references.pmid: 15876430
references.citation: Nature. 1971 Sep 17;233(5316):194-5
references.pmid: 4329470
references.citation: Proteomics. 2003 Nov;3(11):2108-15
references.pmid: 14595809
references.citation: Proteomics. 2001 Oct;1(10):1279-87
references.pmid: 11721639
references.citation: Cancer Res. 2001 Nov 1;61(21):7908-12
references.pmid: 11691811
references.citation: J Immunol. 1999 Jun 1;162(11):6880-92
references.pmid: 10352310
references.citation: Nature. 2003 Mar 13;422(6928):226-32
references.pmid: 12634796
references.citation: Mol Biol Evol. 1998 Aug;15(8):1017-25
references.pmid: 9718729
references.citation: Int J Cancer. 1996 Aug 22;69(4):283-9
references.pmid: 8797869
references.citation: Proteomics Clin Appl. 2007 May;1(5):494-505
references.pmid: 21136701
references.citation: J Cell Biol. 1983 May;96(5):1475-9
references.pmid: 6302102
references.citation: Cancer Sci. 2007 Aug;98(8):1234-40
references.pmid: 17506794
references.citation: J Natl Cancer Inst. 1985 Sep;75(3):527-44
references.pmid: 2411994
references.citation: J Clin Invest. 2001 Nov;108(10):1411-5
references.pmid: 11714730
references.citation: J Immunol. 1994 Jan 15;152(2):754-64
references.pmid: 7904290
references.citation: J Natl Cancer Inst. 1991 Jan 2;83(1):29-36
references.pmid: 1824600
references.citation: Nat Biotechnol. 2003 Jan;21(1):57-63
references.pmid: 12496764
references.citation: Cancer Res. 2007 Dec 15;67(24):12000-6
references.pmid: 18089831
references.citation: J Cell Biochem. 1997 Nov 1;67(2):155-65
references.pmid: 9328821
references.citation: J Proteome Res. 2007 Feb;6(2):751-8
references.pmid: 17269731
references.citation: J Exp Med. 1998 Apr 20;187(8):1163-7
references.pmid: 9547328
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Apr 5;787(1):11-8
references.pmid: 12659729
references.citation: Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9824-9
references.pmid: 11504947
references.citation: Nat Med. 1995 Jul;1(7):701-2
references.pmid: 7585154
references.citation: J Cell Biochem. 1998 Jun 1;69(3):244-51
references.pmid: 9581863
references.citation: Nat Med. 1997 Dec;3(12):1383-8
references.pmid: 9396609
references.citation: Dis Markers. 2004;20(3):149-53
references.pmid: 15502247
references.citation: Mol Cell Proteomics. 2008 Feb;7(2):268-81
references.pmid: 17916591
references.citation: J Immunol. 1971 Aug;107(2):526-33
references.pmid: 4328010
references.citation: Breast Cancer Res Treat. 1998;52(1-3):137-45
references.pmid: 10066078
references.citation: Nature. 1971 Oct 1;233(5318):321-4
references.pmid: 4330069
references.citation: J Exp Med. 1998 Apr 20;187(8):1349-54
references.pmid: 9547346
references.citation: Cancer Lett. 2006 May 8;236(1):64-71
references.pmid: 15992994
references.citation: J Proteome Res. 2004 Mar-Apr;3(2):261-7
references.pmid: 15113102
references.citation: Int J Cancer. 2002 Dec 1;102(4):372-8
references.pmid: 12402307
references.citation: J Natl Cancer Inst. 1992 Apr 15;84(8):602-9
references.pmid: 1556771
references.citation: Int J Oncol. 2001 Nov;19(5):1035-9
references.pmid: 11605006
references.citation: N Engl J Med. 1996 May 2;334(18):1150-5
references.pmid: 8602180
references.citation: EMBO J. 1983;2(6):861-5
references.pmid: 16453457
references.citation: Immunol Today. 1998 Sep;19(9):405-8
references.pmid: 9745203
references.citation: J Immunol. 2006 Jun 1;176(11):6935-44
references.pmid: 16709854
references.citation: Arch Otolaryngol Head Neck Surg. 1993 Nov;119(11):1257-66
references.pmid: 8217085
references.citation: J Immunol. 2006 Mar 1;176(5):2844-56
references.pmid: 16493041
references.citation: Biostatistics. 2003 Jul;4(3):449-63
references.pmid: 12925511
references.citation: Nat Biotechnol. 2003 Jan;21(1):37-8
references.pmid: 12511908
references.citation: Am J Pathol. 1988 Jan;130(1):136-46
references.pmid: 3337209
references.citation: Proc Natl Acad Sci U S A. 1992 May 15;89(10):4368-72
references.pmid: 1374897
references.citation: Cancer Res. 1987 Nov 1;47(21):5691-8
references.pmid: 2959357
references.citation: Int J Cancer. 2004 Feb 10;108(4):558-63
references.pmid: 14696120
references.citation: Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4198-203
references.pmid: 10760287
references.citation: Proc Natl Acad Sci U S A. 1988 Sep;85(17):6394-8
references.pmid: 2970639
references.citation: N Engl J Med. 2005 Sep 22;353(12):1288-90
references.pmid: 16177255
references.citation: J Immunol. 1995 Dec 15;155(12):5719-27
references.pmid: 7499859
references.citation: Lung Cancer. 2005 May;48(2):217-21
references.pmid: 15829321
references.citation: Cancer Res. 1998 Mar 1;58(5):1034-41
references.pmid: 9500467
references.citation: Clin Cancer Res. 1999 Jun;5(6):1393-400
references.pmid: 10389924
references.citation: Clin Cancer Res. 2002 Nov;8(11):3369-76
references.pmid: 12429623
references.citation: J Natl Cancer Inst. 2004 Dec 1;96(23):1743-50
references.pmid: 15572756
references.citation: Anticancer Res. 1999 Nov-Dec;19(6C):5535-42
references.pmid: 10697612
references.citation: J Proteome Res. 2007 Sep;6(9):3558-65
references.pmid: 17696519
references.citation: Br J Cancer. 1995 Oct;72(4):934-8
references.pmid: 7547243
references.citation: Biochemistry. 1989 Sep 5;28(18):7476-86
references.pmid: 2531008