[Role of lipid raft in assembly of human herpesvirus 6].
To explore the role of lipid raft in assembly of human herpesvirus 6, the HHV-6 GS strain was applied to infect the HSB2 cells and then the lipid raft composition was extracted from the cells with non-ionic detergent Triton-X 100. The relationship between the HHV-6 envelope glycoprotein and lipid raft was analyzed by Western Blot. Immunofluorescence double-staining was used to study the colocalization of the HHV-6 glycoprotein B(gB) with GPI anchored protein CD59 and ganglioside GM respectively. HHV-6 envelope glycoprotein B, H, L, Q1 and Q2 (gB, gH, gL, gQ1 and gQ2) were all existed in the lipid raft. Moreover, CD59 and HHV-6 envelope glycoprotein B showed the same localization through the confocal microscope. We concluded the lipid raft provided the platform for HHV-6 assembly. This is the first report concerning to the role of lipid raft in assembly of human Herpesvirus 6.
Huang
Hong-lan
HL
Dept. of Pathogenobiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
Li
Fan
F
D018957:CD59 Antigens / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005677:G(M1) Ganglioside / Q000032:analysis; D015654:Herpesvirus 6, Human / Q000502:physiology*; D021962:Membrane Microdomains / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000032:analysis; D019065:Virus Assembly
D018957:CD59 Antigens; D014759:Viral Envelope Proteins; C077311:glycoprotein B, human herpesvirus-6; D005677:G(M1) Ganglioside