Chemotaxonomy of bacteria by comprehensive GC and GC-MS in electron impact and chemical ionisation mode.
The analysis of the cellular lipidic fraction of bacteria is described. After hydrolysis and methylation, the fatty acid methyl esters (FAMEs) are determined by 1-D GC using the Sherlock MIDI bacteria identification system, by comprehensive GC (GC x GC) and by GC-MS in electron impact (EI) and positive chemical ionisation (PCI) mode. With GC x GC, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. GC-EI-MS and GC-PCI-MS were helpful for confirmation. The bacteria selected in this study are Brevundimonas diminuta, Chryseobacterium gleum and Stenotrophomonas maltophilia.
David
Frank
F
Research Institute for Chromatography, Kortrijk, Belgium.
Tienpont
Bart
B
Sandra
Pat
P
D000818:Animals; D001419:Bacteria / Q000737:chemistry* / Q000145:classification; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*