Generation of plants resistant to tomato yellow leaf curl virus by using artificial zinc-finger proteins.
Previously, we designed an artificial zinc-finger protein (AZP) for blocking a replication protein (Rep) of beet severe curly top virus (BSCTV) from binding to its replication origin and demonstrated that transgenic Arabidopsis plants expressing the AZP are completely resistant to the virus infection. Here we applied the AZP technology to tomato yellow leaf curl virus (TYLCV) infective to an important agricultural crop, tomato. We designed and constructed an AZP binding to the direct repeat to block the TYLCV Rep binding. In gel shift assays, we confirmed that the designed AZP has a higher affinity to the replication origin than that of Rep and that the AZP effectively inhibited the Rep binding to its replication origin in vitro. The AZP gene was then introduced into a plant genome with the help of Agrobacterium tumefaciens to generate the transgenic plants. We will discuss properties of the AZP-transgenic plants against TYLCV infection.
Koshino-Kimura
Yoshihiro
Y
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyotodaigaku-Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Takenaka
Kosuke
K
Domoto
Fumiya
F
Aoyama
Yasuhiro
Y
Sera
Takashi
T
D017360:Arabidopsis / Q000235:genetics / Q000821:virology; D053839:Begomovirus / Q000235:genetics*; D001667:Binding, Competitive; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified / Q000821:virology; D011994:Recombinant Proteins / Q000378:metabolism; D018741:Replication Origin; D014764:Viral Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins