[The effect of basic fibroblast growth factor on the gene expression of decorin by periodontal ligament fibroblasts in culture].
OBJECTIVE
To study the effect of basic fibroblast growth factor (bFGF) on the gene expression of decorin by periodontal ligament fibroblasts (PLFs) in culture, and discuss the effect of bFGF in periodontal regeneration.


METHODS
Human PLFs were cultured and stimulated by exogenous bFGF. Gene expression of decorin was assessed by semi-quantitive RT-PCR.


RESULTS
The mRNA expression of decorin was suppressed by bFGF and the effect was dose-dependent. When the dose of bFGF increased, the inhibitive effect decreased.


CONCLUSIONS
Decorin has many biological effects. The inhibitive effect may be one of important factors which participate in the healing process of periodontitis, and provide partly theoretical basis of bFGF in periodontal regeneration.
Wang
Si-cong
SC
Dept. of Periodontology, Stomatology Hospital of Jilin University, Changchun 130041, China.
Lin
Chong-tao
CT
Nie
Dai-bang
DB
Ouyang
Hong-sheng
HS
D058575:Decorin; D016222:Fibroblast Growth Factor 2; D005347:Fibroblasts; D006801:Humans; D010513:Periodontal Ligament; D012333:RNA, Messenger; D012038:Regeneration
C546878:DCN protein, human; D058575:Decorin; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2