[Antitumor effects of raddeanin A on S180, H22 and U14 cell xenografts in mice].
OBJECTIVE
Raddeanin A, a triterpenoid saponin from Anemone raddeana Regel, has good antitumor activity in vitro. This study was to investigate its antitumor effects on tumor cell xenografts in mice.


METHODS
The inhibitory effects of raddeanin A on the proliferation of human nasopharyngeal carcinoma KB cells and ovarian cancer SKOV3 cells were measured by MTT assay. The inhibitory effects of raddeanin A injection on the growth of sarcoma S180, liver cancer H22 and cervical carcinoma U14 cell xenografts in mice and the effect of raddeanin A lavage on the growth of S180 cell xenografts were measured. The acute toxicity of raddeanin A was also measured.


RESULTS
The 50% inhibition concentration (IC(50)) of raddeanin A was 4.64 microg/mL for KB cells and 1.40 microg/mL for SKOV3 cells. When injected with raddeanin A at a dose of 4.5 mg/kg, the growth inhibition rates of S180, H22 and U14 cell xenografts were 60.5%, 36.2% and 61.8%, respectively. When lavaged with raddeanin A at a dose of 200 mg/kg, the growth inhibition rate of S180 cell xenografts was 64.7%. The median lethal dose (LD50) of raddeanin A lavage was 1.1 g/kg and that of raddeanin A injection was 16.1 mg/kg.


CONCLUSIONS
Raddeanin A has good antitumor activity both in vitro and in vivo, and would be a potential antitumor medicine.
Wang
Ming-Kui
MK
Natural Product Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, 610041, PR China. wangmk@cib.ac.cn
Ding
Li-Sheng
LS
Wu
Feng-E
FE
D031883:Anemone / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009303:Nasopharyngeal Neoplasms / Q000473:pathology; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D012510:Sarcoma 180 / Q000473:pathology*; D047368:Tumor Burden / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal