title: A novel septin-associated protein, Syp1p, is required for normal cell cycle-dependent septin cytoskeleton dynamics in yeast.
abstract: Septins are a family of GTP-binding proteins whose heterooligomeric complex is the basic structural element of the septin filaments found in many eukaryotic organisms. In budding yeast, septins are mainly confined at the mother-daughter junction and are required for cell morphogenesis and division. Septins undergo assembly and disassembly in accordance with the progression of the cell cycle. In this report, we identified the yeast protein Syp1p as a new regulator of septin dynamics. Syp1p colocalizes with septins throughout most of the cell cycle. Syp1p interacts with the septin subunit Cdc10p and can be precipitated by Cdc10p and Cdc12p. In the syp1Delta mutant, both formation of a complete septin ring at the incipient bud site and disassembly of the septin ring in later stages of cell division are significantly delayed. In addition, overexpression of Syp1p causes marked acceleration of septin disassembly. The fluorescence recovery after photobleaching (FRAP) assay further showed that Syp1p promotes septin turnover in different cell cycle stages. These results suggest that Syp1p is involved in the regulation of cell cycle-dependent dynamics of the septin cytoskeleton in yeast.
authors.affiliation: Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Qiu
authors.forename: Suat Peng
authors.initials: SP
authors.lastname: Neo
authors.forename: Xianwen
authors.initials: X
authors.lastname: Yu
authors.forename: Mingjie
authors.initials: M
authors.lastname: Cai
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002455:Cell Division; D022082:Cellular Structures; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D003599:Cytoskeleton / Q000502:physiology*; D036681:Fluorescence Recovery After Photobleaching; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics; D020798:Two-Hybrid System Techniques
chemical_list: C099504:CDC12 protein, S cerevisiae; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; C512892:CDC10 protein, S cerevisiae; D020558:GTP Phosphohydrolases
references.citation: Mol Cell Biol. 1997 Jun;17(6):3242-53
references.pmid: 9154823
references.citation: Trends Cell Biol. 1999 Oct;9(10):387-94
references.pmid: 10481176
references.citation: Trends Cell Biol. 2005 Aug;15(8):414-24
references.pmid: 16009555
references.citation: Mol Biol Cell. 2001 Dec;12(12):3759-72
references.pmid: 11739778
references.citation: J Cell Sci. 2001 Apr;114(Pt 7):1379-86
references.pmid: 11257003
references.citation: Oncogene. 1999 Nov 25;18(50):7046-54
references.pmid: 10597305
references.citation: J Cell Biol. 2002 Jan 21;156(2):315-26
references.pmid: 11807094
references.citation: Mol Biol Cell. 2003 Jan;14(1):26-39
references.pmid: 12529424
references.citation: J Cell Biol. 1998 Nov 2;143(3):719-36
references.pmid: 9813093
references.citation: Science. 2004 Jul 16;305(5682):393-6
references.pmid: 15256669
references.citation: Microbiology (Reading). 2001 Jun;147(Pt 6):1437-1450
references.pmid: 11390675
references.citation: J Cell Biol. 1998 Dec 28;143(7):1947-60
references.pmid: 9864366
references.citation: J Cell Biol. 2004 Mar 1;164(5):701-15
references.pmid: 14993234
references.citation: Mol Cell Biol. 1996 Apr;16(4):1857-70
references.pmid: 8657162
references.citation: Nature. 2007 Sep 20;449(7160):311-5
references.pmid: 17637674
references.citation: Exp Cell Res. 1971 Dec;69(2):265-76
references.pmid: 4950437
references.citation: Mol Biol Cell. 1997 Jun;8(6):987-98
references.pmid: 9201710
references.citation: J Cell Biol. 1993 Jul;122(2):373-86
references.pmid: 8320260
references.citation: Mol Biol Cell. 2002 Oct;13(10):3477-92
references.pmid: 12388751
references.citation: J Cell Biol. 2005 Jun 20;169(6):897-908
references.pmid: 15967812
references.citation: Mol Biol Cell. 2004 Dec;15(12):5329-45
references.pmid: 15371547
references.citation: Mol Cell Biol. 2000 Jun;20(11):4049-61
references.pmid: 10805747
references.citation: Nat Cell Biol. 2000 Oct;2(10):677-85
references.pmid: 11025657
references.citation: Science. 2000 Oct 13;290(5490):341-4
references.pmid: 11030653
references.citation: Genetics. 2000 Oct;156(2):579-92
references.pmid: 11014808
references.citation: Genes Dev. 1999 Jan 15;13(2):176-87
references.pmid: 9925642
references.citation: Mol Biol Cell. 2004 Oct;15(10):4568-83
references.pmid: 15282341
references.citation: Cell Struct Funct. 2001 Dec;26(6):529-37
references.pmid: 11942606
references.citation: Nature. 2006 Sep 28;443(7110):466-9
references.pmid: 17006515
references.citation: Mol Biol Cell. 2003 Oct;14(10):4051-66
references.pmid: 14517318
references.citation: Dev Cell. 2003 Mar;4(3):345-57
references.pmid: 12636916
references.citation: FEMS Yeast Res. 2007 Sep;7(6):782-95
references.pmid: 17425670
references.citation: Mol Cell Biol. 2001 Jan;21(2):488-500
references.pmid: 11134337
references.citation: J Cell Biol. 1997 Oct 6;139(1):75-93
references.pmid: 9314530
references.citation: Curr Opin Cell Biol. 1996 Feb;8(1):106-19
references.pmid: 8791410
references.citation: Curr Opin Cell Biol. 2003 Dec;15(6):648-53
references.pmid: 14644188
references.citation: J Cell Sci. 2006 Jan 1;119(Pt 1):4-10
references.pmid: 16371649
references.citation: Mol Cell. 2000 May;5(5):841-51
references.pmid: 10882120
references.citation: Genes Dev. 1995 Aug 1;9(15):1817-30
references.pmid: 7649470
references.citation: Mol Biol Cell. 2006 Mar;17(3):1110-25
references.pmid: 16371506
references.citation: Cell Cycle. 2002 Jan;1(1):42-9
references.pmid: 12429908