Targeted antimicrobial activity of a specific IgG-SMAP28 conjugate against Porphyromonas gingivalis in a mixed culture.
Antimicrobial peptides coupled to a ligand, receptor or antibody for a specific pathogenic bacteria could be used to develop narrow-spectrum pharmaceuticals with 'targeted' antimicrobial activity void of adverse reactions often associated with the use of broad-spectrum antibiotics. To assess the feasibility of this approach, in this study sheep myeloid antimicrobial peptide (SMAP) 28 was linked to affinity- and protein G-purified rabbit immunoglobulin G (IgG) antibodies specific to the outer surface of Porphyromonas gingivalis strain 381. The selective activity of the P. gingivalis IgG-SMAP28 conjugate was then assessed by adding it to an artificially generated microbial community containing P. gingivalis, Aggregatibacter actinomycetemcomitans and Peptostreptococcus micros. The specificity of the P. gingivalis IgG-SMAP28 conjugate in this mixed culture was concentration-dependent. The conjugate at 50 microg protein/mL lacked specificity and killed P. gingivalis, A. actinomycetemcomitans and P. micros. The conjugate at 20 microg protein/mL was more specific and killed P. gingivalis. This is an initial step to develop a selective antimicrobial agent that can eliminate a specific periodontal pathogen, such as P. gingivalis, from patients with periodontal disease without harming the normal commensal flora.
Franzman
Michael R
MR
Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
Burnell
Kindra K
KK
Dehkordi-Vakil
Farideh H
FH
Guthmiller
Janet M
JM
Dawson
Deborah V
DV
Brogden
Kim A
KA
D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D000918:Antibody Specificity; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003470:Culture Media; D017753:Ecosystem; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology*; D008301:Maleimides / Q000737:chemistry; D008826:Microbial Sensitivity Tests / Q000379:methods; D015661:Pasteurellaceae / Q000254:growth & development; D010462:Peptostreptococcus / Q000254:growth & development; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D012756:Sheep
D000907:Antibodies, Bacterial; D023181:Antimicrobial Cationic Peptides; D003432:Cross-Linking Reagents; D003470:Culture Media; D007074:Immunoglobulin G; D008301:Maleimides; C043592:maleimide
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