[Effect of petroleum ether extract from Melilotus suaveolens Ledeb on the expression of NF-kappaB and Heme oxygenase 1].
OBJECTIVE
To study the anti-inflammatory effect of petroleum ether extract from Melilotus suaveolens Ledeb.


METHODS
Inflammatory cell model was constructed by LPS acting on the RAW264.7 cell line. The expression and distribution of NF-kappaB were detected using immunocytochemical method. The expression of mRNA and protein of Heme oxygenase 1(HO-1) were detected by real-time PCR and Western blot.


RESULTS
The immunocytochemical analysis showed that the cytoplasm stained to brown presented NF-kappaB inactivation after the intervention of petroleum ether extract while the cell nucleus stained to brown presented NF-kappaB activation after the only intervention of LPS. The expression of HO-1 mRNA was significantly enhanced by the extract in a dose-dependent manner, and the expression of HO-1 protein was markedly enhanced too.


CONCLUSIONS
The petroleum ether extract from Melilotus suaveolens Ledeb can resist inflammation by inhibiting the activation of proinflammatory factor NF-kappaB and enhancing the expression of anti-inflammatory factor HO-1.
Pang
Ran
R
Department of Infectious Diseases, Union Hospital; Huazhong University of Science and Technology, Wuhan 430022, China.
Zhang
Shu-ling
SL
Zhao
Lei
L
Liu
Shuang-lin
SL
Dong
Ji-hua
JH
Tao
Jun-yan
JY
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004986:Ether / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects*; D051547:Heme Oxygenase-1 / Q000235:genetics* / Q000276:immunology; D007249:Inflammation / Q000188:drug therapy / Q000235:genetics / Q000276:immunology; D029903:Melilotus / Q000737:chemistry*; D051379:Mice; D016328:NF-kappa B / Q000235:genetics* / Q000276:immunology; D010936:Plant Extracts / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D016328:NF-kappa B; D010936:Plant Extracts; D004986:Ether; D051547:Heme Oxygenase-1