title: Characterization and membrane gene-based phylogenetic analysis of avian infectious bronchitis virus Chinese strain HH06.
abstract: A Chinese isolate of avian infectious bronchitis virus (IBV) designated HH06 was isolated from the kidney tissues of a chicken flock experiencing an outbreak of nephritis. In vivo pathogenicity of the IBV isolate HH06 was determined by inoculating specific pathogen-free (SPF) chickens. The clinical signs and related gross lesions of HH06 infected chickens were similar with those of the field-infected chickens. SPF embryonated eggs were inoculated with virus suspension for serial passage and their genomic RNA was extracted. RT-PCR technique was utilized to amplify the M gene sequence encoding membrane protein of IBV. Recombinant plasmid named T-vector-M was constructed via inserting the M gene into the TA cloning vector, pMD 18-T. The sequenced M gene and its deduced amino acid (aa) sequences were compared with the published sequences of reference strains. The M gene is of 687 bp in length encoding the M protein of 228 amino acids with a predicted molecular weight of 25.4 kDa. The sequences of the M gene and M protein share 83.9-97.9% and 83.6-96.5% homologous identities, respectively, compared with 29 IBV reference strains derived from different regions or countries, which revealed that there are still significant variations between strains. Furthermore, a phylogenetic tree based on these M DNA sequences was generated, and the tree topology suggests that some Chinese IBV strains may have a common ancestor; however, HH06 is a new local IBV isolate that is responsible for the field outbreak of nephritis.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030, Harbin, People's Republic of China. rxfemail@yahoo.com.cn
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
authors.forename: Jiechao
authors.initials: J
authors.lastname: Yin
authors.forename: Dexing
authors.initials: D
authors.lastname: Ma
authors.forename: Guangxing
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D002681:China; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D000086862:Coronavirus M Proteins; D004196:Disease Outbreaks; D001351:Infectious bronchitis virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009393:Nephritis / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D000086862:Coronavirus M Proteins; C000706230:M protein, Avian infectious bronchitis virus; D012367:RNA, Viral; D014763:Viral Matrix Proteins
references.citation: Adv Virus Res. 1997;48:1-100
references.pmid: 9233431
references.citation: J Gen Virol. 2005 Mar;86(Pt 3):719-725
references.pmid: 15722532
references.citation: J Gen Virol. 1988 Mar;69 ( Pt 3):621-9
references.pmid: 2832526
references.citation: Avian Pathol. 2004 Jun;33(3):321-7
references.pmid: 15223561
references.citation: J Clin Microbiol. 2005 Apr;43(4):1982-4
references.pmid: 15815038
references.citation: Avian Dis. 2001 Apr-Jun;45(2):416-24
references.pmid: 11417821
references.citation: Avian Pathol. 2005 Dec;34(6):439-48
references.pmid: 16537157
references.citation: Adv Exp Med Biol. 1990;276:369-72
references.pmid: 1966424
references.citation: Virus Res. 2004 Mar 15;100(2):191-8
references.pmid: 15019237
references.citation: Avian Dis. 2007 Jun;51(2):527-33
references.pmid: 17626478
references.citation: Virology. 1992 Aug;189(2):792-4
references.pmid: 1322604
references.citation: J Virol. 1988 Jan;62(1):8-11
references.pmid: 2824858
references.citation: J Gen Virol. 1983 May;64(Pt 5):1187-91
references.pmid: 6302214
references.citation: Semin Virol. 1997 Feb;8(1):33-47
references.pmid: 32288441
references.citation: Arch Virol. 2006 Jun;151(6):1133-48
references.pmid: 16397751
references.citation: Gene. 1988 Dec 15;73(1):237-44
references.pmid: 3243435
references.citation: J Virol. 1998 Nov;72(11):8636-43
references.pmid: 9765403
references.citation: J Virol. 2003 Aug;77(16):9084-9
references.pmid: 12885925
references.citation: Arch Virol. 2004 Oct;149(10):2069-78
references.pmid: 15290359
references.citation: J Virol Methods. 2005 Feb;123(2):203-11
references.pmid: 15620403
references.citation: J Virol. 2008 Feb;82(4):2013-24
references.pmid: 18045937
references.citation: Vet Microbiol. 1993 Nov;37(3-4):285-97
references.pmid: 8116187
references.citation: J Virol. 1992 Feb;66(2):743-9
references.pmid: 1309909
references.citation: Adv Exp Med Biol. 1993;342:305-10
references.pmid: 8209748
references.citation: Virus Genes. 2007 Jun;34(3):327-41
references.pmid: 16927130
references.citation: Vaccine. 2007 Jul 26;25(30):5558-62
references.pmid: 17416443
references.citation: J Virol. 2005 Jul;79(13):8065-78
references.pmid: 15956552
references.citation: J Biol Chem. 1995 Apr 14;270(15):8815-21
references.pmid: 7721788
references.citation: J Vet Med B Infect Dis Vet Public Health. 2004 May;51(4):147-52
references.pmid: 15228547
references.citation: J Biol Chem. 1992 Jul 15;267(20):14094-101
references.pmid: 1629209
references.citation: Avian Pathol. 2006 Oct;35(5):394-9
references.pmid: 16990149
references.citation: J Virol. 2000 May;74(9):4319-26
references.pmid: 10756047