Simultaneous doping analysis of main urinary metabolites of anabolic steroids in horse by ion-trap gas chromatography-tandem mass spectrometry.
The use of anabolic steroids in racehorses is strictly regulated. We have developed a method for the simultaneous analysis of 11 anabolic steroids: fluoxymesterone, 17alpha-methyltestosterone, mestanolone, methandienone, methandriol, oxymetholone, boldenone, furazabol, methenolone, nandrolone, and stanozolol, for possible application to a doping test in racehorses. We selected 15 kinds of target substances for a doping test from the main metabolites of these anabolic steroids, and established a method for simultaneous analysis. Urine was hydrolyzed and subjected to solid-phase extraction. Then, the residue from the extracts was derivatized by trimethylsilylation. The derivatized samples were subjected to ion-trap gas chromatography-tandem mass spectrometry, and their mass chromatograms and product ion spectra were obtained. The limit of detection of the target substances was 5-50 ng/mL, and the mean recovery and coefficient of variation were 71.3-104.8% and 1.1-9.5%, respectively.
Yamada
Masayuki
M
Laboratory of Racing Chemistry, Utsunomiya, Tochigi 320-0851, Japan. m-yamada@lrc.or.jp
Aramaki
Sugako
S
Kurosawa
Masahiko
M
Kijima-Suda
Isao
I
Saito
Koichi
K
Nakazawa
Hiroyuki
H
D045930:Anabolic Agents / Q000378:metabolism* / Q000652:urine*; D000818:Animals; D002849:Chromatography, Gas; D004300:Doping in Sports; D005260:Female; D006736:Horses / Q000378:metabolism* / Q000652:urine*; D008297:Male; D015203:Reproducibility of Results; D013256:Steroids / Q000378:metabolism* / Q000652:urine*; D053719:Tandem Mass Spectrometry; D013997:Time Factors
D045930:Anabolic Agents; D013256:Steroids