Cultivation, LD(50) determination and experimental model of Streptococcus suis serotype 2 strain HA9801.
The effects of nutritional components and submerged culture conditions on colony-forming unit (CFU) counts by Streptococcus suis serotype 2 strain HA9801 in flask culture was investigated, and the optimal medium and cultivation conditions was confirmed by using a 50l bioreactor. The LD(50) values of HA9801 in pigs before and after fermentation were 1.8 x 10(7)CFU, which indicated that the virulence of HA9801 was very stable in the fermentation process. In addition, an experimental model that closely mimics naturally occurring disease in conventional pigs was established.
Zhao
Zhanzhong
Z
Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200232, China.
Wang
Jian
J
Liu
Peihong
P
Zhang
Suhua
S
Gong
Jianpei
J
Huang
Xiqin
X
Li
Bin
B
Xue
Feiqun
F
D000818:Animals; D019149:Bioreactors; D015169:Colony Count, Microbial / Q000662:veterinary; D007928:Lethal Dose 50; D008297:Male; D011897:Random Allocation; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D017011:Streptococcus suis / Q000254:growth & development* / Q000472:pathogenicity*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014774:Virulence