title: A perivascular origin for mesenchymal stem cells in multiple human organs.
abstract: Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-Rbeta expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.
authors.affiliation: Department of Pediatrics, Stem Cell Research Center, Children's Hospital of Pittsburgh, Pittsburgh, PA 15213, USA.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Crisan
authors.forename: Solomon
authors.initials: S
authors.lastname: Yap
authors.forename: Louis
authors.initials: L
authors.lastname: Casteilla
authors.forename: Chien-Wen
authors.initials: CW
authors.lastname: Chen
authors.forename: Mirko
authors.initials: M
authors.lastname: Corselli
authors.forename: Tea Soon
authors.initials: TS
authors.lastname: Park
authors.forename: Gabriella
authors.initials: G
authors.lastname: Andriolo
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Cyrille
authors.initials: C
authors.lastname: Norotte
authors.forename: Pang-Ning
authors.initials: PN
authors.lastname: Teng
authors.forename: Jeremy
authors.initials: J
authors.lastname: Traas
authors.forename: Rebecca
authors.initials: R
authors.lastname: Schugar
authors.forename: Bridget M
authors.initials: BM
authors.lastname: Deasy
authors.forename: Stephen
authors.initials: S
authors.lastname: Badylak
authors.forename: Hans-Jörg
authors.initials: HJ
authors.lastname: Buhring
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Giacobino
authors.forename: Lorenza
authors.initials: L
authors.lastname: Lazzari
authors.forename: Johnny
authors.initials: J
authors.lastname: Huard
authors.forename: Bruno
authors.initials: B
authors.lastname: Péault
mesh_terms: D000293:Adolescent; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology*; D000368:Aged; D002465:Cell Movement; D053686:Fetal Stem Cells / Q000166:cytology*; D005333:Fetus; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D024510:Muscle Development; D020286:Pericytes / Q000166:cytology*