title: The role of Pm-fortilin in protecting shrimp from white spot syndrome virus (WSSV) infection.
abstract: Crustacean fortilin or the product of the translationally controlled tumor protein (TCTP) gene isolated from Penaeus monodon, is well conserved and has a Ca(++) binding domain. Pm-fortilin has anti-apoptotic properties and is present at high levels during the onset of viral infections in P. monodon. The possibility of using rFortilin to protect against white spot syndrome virus (WSSV) infection was tested. Injection of shrimp with rFortilin, after infection with WSSV, resulted in 80-100% survival and detection of very low levels of WSSV by PCR, whereas in moribund samples WSSV levels were very high. This result implies that injection of recombinant rFortilin decreases viral infection by an unknown mechanism, but probably by inhibiting viral replication. Using a yeast two-hybrid screen for cellular protein partners to rFortilin we identified an unknown protein that bound to fortilin. This is a novel polypeptide of 93 amino acids with a number of XPPX signature sequences that are often reported to have a function in antiviral peptides.
authors.affiliation: Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.
authors.forename: Moltira
authors.initials: M
authors.lastname: Tonganunt
authors.forename: Benjamas
authors.initials: B
authors.lastname: Nupan
authors.forename: Manasawan
authors.initials: M
authors.lastname: Saengsakda
authors.forename: Sawitree
authors.initials: S
authors.lastname: Suklour
authors.forename: Warapond
authors.initials: W
authors.lastname: Wanna
authors.forename: Saengchan
authors.initials: S
authors.lastname: Senapin
authors.forename: Wilaiwan
authors.initials: W
authors.lastname: Chotigeat
authors.forename: Amornrat
authors.initials: A
authors.lastname: Phongdara
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D004266:DNA Virus Infections / Q000517:prevention & control / Q000662:veterinary*; D033561:Penaeidae; D000090324:Tumor Protein, Translationally-Controlled 1; D046848:White spot syndrome virus 1
chemical_list: D014408:Biomarkers, Tumor; D000090324:Tumor Protein, Translationally-Controlled 1