Two distinct T cell subsets, CD4+ and CD8+CD60+, and their cytokines are required for in vitro induction of human ragweed-specific memory IgE responses.
CD8(+)CD60(+) T cells (80-98% CD45RO(+); 20% CD23(+)) are significantly increased in the blood of serum IgE(+) ragweed-sensitized (RS) compared with serum IgE-nonatopic humans (p = 0.001). CD8(+)CD60(+) T cells of the RS patients produced IL-2, IL-4, IL-10, IL-12, IFN-alpha. and IFN-gamma, but not IL-6 or IL-13. When their PBMC were cultured with ragweed Ag (RA), peak IgE responses occurred on day 10; none was induced with non-cross-reacting or without Ag; nonatopic PBMC did not respond to any stimulant. When either CD4(+) or CD8(+)CD60(+) T cells were depleted from RS PBMC before culture with RA, no IgE responses were induced. If purified CD4(+) T cells or low numbers of CD8(+)CD60(+) T cells were added back to the depleted PBMC, IgE responses were restored. However, higher numbers of CD8(+)CD60(+) T cells totally suppressed IgE responses. Total suppression also was obtained when RS PBMC were cultured with RA and either anti-IL-2, IL-4, IL-10, IL-12, IFN-gamma (all concentrations), or IFN-alpha (low concentrations), but not anti-IL-6 or IL-13. Higher concentrations of anti-IFN-alpha potentiated IgE responses.
Smith-Norowitz
Tamar A
TA
Center for Allergy and Asthma Research at State University of New York Downstate, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA.
Silverberg
Jonathan
J
Norowitz
Kevin B
KB
Bluth
Martin H
MH
Chice
Seto
S
Joks
Rauno
R
Nowakowski
Maja
M
Durkin
Helen G
HG
D000328:Adult; D031164:Ambrosia / Q000276:immunology*; D015703:Antigens, CD / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000502:physiology*; D018984:Epitopes, T-Lymphocyte / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology / Q000378:metabolism; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood; D007156:Immunologic Memory / Q000276:immunology; D007165:Immunosuppression Therapy; D016898:Interferon-alpha / Q000502:physiology; D017493:Leukocyte Common Antigens / Q000096:biosynthesis / Q000097:blood; D008297:Male; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; C064741:CDw60 antigen; D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D016898:Interferon-alpha; D007073:Immunoglobulin E; D017493:Leukocyte Common Antigens