Immunohistochemical evidence of ubiquitous distribution of the metalloendoprotease insulin-degrading enzyme (IDE; insulysin) in human non-malignant tissues and tumor cell lines.
Immunohistochemical evidence of ubiquitous distribution of the metalloprotease insulin-degrading enzyme (IDE; insulysin) in human non-malignant tissues and tumor cells is presented. Immunohistochemical staining was performed on a multi-organ tissue microarray (pancreas, lung, kidney, central/peripheral nervous system, liver, breast, placenta, myocardium, striated muscle, bone marrow, thymus, and spleen) and on a cell microarray of 31 tumor cell lines of different origin, as well as trophoblast cells and normal blood lymphocytes and granulocytes. IDE protein was expressed in all the tissues assessed and all the tumor cell lines except for Raji and HL-60. Trophoblast cells and granulocytes, but not normal lymphocytes, were also IDE-positive.
Weirich
Gregor
G
Institute of Pathology, Klinikum rechts der Isar, Technical University of Munich, Ismaninger Str. 22, D-81675 Munich, Germany.
Mengele
Karin
K
Yfanti
Christina
C
Gkazepis
Apostolos
A
Hellmann
Daniela
D
Welk
Anita
A
Giersig
Cecylia
C
Kuo
Wen-Liang
WL
Rosner
Marsha Rich
MR
Tang
Wei-Jen
WJ
Schmitt
Manfred
M
D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007339:Insulysin / Q000032:analysis / Q000097:blood / Q000276:immunology* / Q000378:metabolism*; D009928:Organ Specificity
D007339:Insulysin
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