title: Metagenomic screening for bleomycin resistance genes.
abstract: A metagenomic library of activated sludge was screened for bleomycin resistance genes. Two genes were identified that differed greatly from each other, from the genes of bleomycin-producing actinomycetes, and from those of clinical isolates. Therefore, the nonclinical environment is a rich reservoir of new resistance elements, and metagenomics can be used to sample the resistome rapidly.
authors.affiliation: Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Mori
authors.forename: Shiori
authors.initials: S
authors.lastname: Mizuta
authors.forename: Hikaru
authors.initials: H
authors.lastname: Suenaga
authors.forename: Kentaro
authors.initials: K
authors.lastname: Miyazaki
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001419:Bacteria / Q000235:genetics*; D001761:Bleomycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D017385:Sequence Homology; D012722:Sewage / Q000382:microbiology*
chemical_list: D000903:Antibiotics, Antineoplastic; D012722:Sewage; D001761:Bleomycin
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