Preparation of F0F1 -ATPase nanoarray by dip-pen nanolithography and its application as biosensors.
Dip-pen nanolithography (DPN) is a widely used technique to create nanoscopic patterns of many different materials. F(0)F(1) -ATPase is a nanoscale rotary molecular motor, and could be used as a biosensor or an ideal motor device in a micro-/nanosystem. In this paper, the DPN technique was used to create nanoarrays of F(0)F(1) -ATPase within chromatophore on a gold surface. The feature size of our F(0)F(1) -ATPase patterns was an average of 130 nm, and mathematically, there were no more than ten F(0)F(1) -ATPases in each dot. The biological activity of patterned F(0)F(1) -ATPase was demonstrated by its adenosine triphosphate synthesis, which was indicated by the fluorescence change of labeled F1300. The patterned F(0)F(1) -ATPase nanoarrays were further constructed as biosensors to detect H9 influenza A virus. The results showed that the biosensor arrays had a remarkable S/N ratio and excellent specificity. This type of biosensor arrays can be further used in high-throughput, high-sensitive detection in future. Meanwhile, the precise patterning of F(0)F(1) -ATPase with desired size, position, and biological activity would accelerate its application in many fields.
Li
Zheng
Z
Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China. james.lizheng@gmail.com
Liu
Xiaolong
X
Zhang
Zhenxi
Z
D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D053127:Influenza A Virus, H9N2 Subtype / Q000302:isolation & purification*; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D010781:Photography / Q000295:instrumentation / Q000379:methods; D006180:Proton-Translocating ATPases / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D006046:Gold; D006180:Proton-Translocating ATPases