African trypanosomes contain 5-methylcytosine in nuclear DNA.
It is currently unclear if there are modified DNA bases in Trypanosoma brucei other than J-base. We identify herein a cytosine-5 DNA methyltransferase gene and report the presence and location of 5-methylcytosine in genomic DNA. Our data demonstrate that African trypanosomes contain a functional cytosine DNA methylation pathway.
Militello
Kevin T
KT
Department of Biology, State University of New York at Geneseo, Geneseo, NY 14454, USA. militello@geneseo.edu
Wang
Ping
P
Jayakar
Sangeeta K
SK
Pietrasik
Rebecca L
RL
Dupont
Christopher D
CD
Dodd
Kristi
K
King
Anthony M
AM
Valenti
Paul R
PR
D044503:5-Methylcytosine / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D016054:DNA, Protozoan / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D044503:5-Methylcytosine; D004248:DNA (Cytosine-5-)-Methyltransferases
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