title: Mutation analysis of the base-pair connecting two functional modules in the DSL ribozyme.
abstract: The class DSL ribozyme is one of artificial RNA enzymes generated by module-based molecular design. In the structure of this ribozyme, two most important functional modules are connected by a U-A base-pair. We have examined the possible importance of this base-pair by site-directed mutation experiments using the DSL-1S ribozyme and its derivative possessing altered modular organization. The analysis indicated that the DSL-1S ribozyme preferred U-A pair at the positions whereas the derivative preferred A-U pair.
authors.affiliation: Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 819-0395, Fukuoka, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Ishikawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Furuta
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Ikawa
mesh_terms: D000225:Adenine / Q000737:chemistry; D020029:Base Pairing; D001483:Base Sequence; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016337:RNA, Catalytic / Q000737:chemistry*; D014498:Uracil / Q000737:chemistry
chemical_list: D016337:RNA, Catalytic; D014498:Uracil; D000225:Adenine