Expression of stilbene synthase gene in transgenic tomato using salicylic acid-inducible Cre/loxP recombination system with self-excision of selectable marker.
A plant transformation vector, pCLKSCLA25 (EU327498), was developed to contain eight cloning sites and the inducible self-excision system which provided an effective approach to eliminate the selectable marker gene(s) from transgenic plants. Upon induction by salicylic acid, the cre gene produced a recombinase that eliminated sequences encoding the selectable marker neomycin phosphotransferase and cre itself. The excision efficiency was 41% in transgenic tomato regenarants. The stilbene synthase gene (vst1) from Vitis vinifera L. was cloned into pCLKSCLA25. The expression of vst1 gene contributed to the accumulation of trans-reveratrol from 3.4 to 8.7 mug/g fresh wt in different marker-free transgenic tomato lines.
Ma
B G
BG
Key Oasis Eco-agriculture Laboratory of Xinjiang Production and Construction Group, Agricultural College, Shihezi University, Shihezi 832000, People's Republic of China. mbg_agr@shzu.edu.cn
Duan
X Y
XY
Niu
J X
JX
Ma
C
C
Hao
Q N
QN
Zhang
L X
LX
Zhang
H P
HP
D000217:Acyltransferases / Q000235:genetics*; D015139:Blotting, Southern; D018744:DNA, Plant / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005819:Genetic Markers; D019426:Integrases / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011995:Recombination, Genetic / Q000187:drug effects*; D020156:Salicylic Acid / Q000494:pharmacology*
D018744:DNA, Plant; D005819:Genetic Markers; D010940:Plant Proteins; D000217:Acyltransferases; C022674:stilbene synthase; C045073:Cre recombinase; D019426:Integrases; D020156:Salicylic Acid