title: Hyperbaric oxygen treatment improves GFR in rats with ischaemia/reperfusion renal injury: a possible role for the antioxidant/oxidant balance in the ischaemic kidney.
abstract: BACKGROUND
  Ischaemic kidney injury continues to play a dominant role in the pathogenesis of acute renal failure (ARF) in many surgical and medical settings. A major event in the induction of renal injury is related to the generation of oxygen-free radicals. Hyperbaric oxygen therapy (HBO) is indicated for treatment of many ischaemic events but not for ARF. Therefore, the present study examined the effects of HBO on kidney function and renal haemodynamics in rats with ischaemic ARF.
  
  
  METHODS
  Renal ischaemia was induced by unilateral renal artery clamping (45 min) in rats. Within 24 h following ischaemia, rats were treated twice with HBO of 100% O(2) at 2.5 absolute atmospheres for 90 min each (+HBO). Untreated rats (-HBO) served as a control. Forty-eight hours later, GFR, RBF and endothelial-dependent vasorelaxation were measured. In addition, the immunoreactive staining of 4-hydroxy-2-noneal (4-HNE), a major product of endogenous lipid peroxidation, and superoxide dismutase (SOD) were assessed.
  
  
  RESULTS
  In the -HBO group, GFR was reduced by 94% compared with the untouched normal kidney (ischaemic: 0.06 +/- 0.03 ml/min, normal: 1.02 +/- 0.13 ml). In contrast, in the +HBO group, GFR of the ischaemic kidney (0.36 +/- 0.07 ml/min) was reduced only by 68% compared with the contralateral normal kidney (1.12 +/- 0.12 ml/min). In line with these findings, HBO improved the vasodilatory response to ACh as expressed in enhancement of both total and regional renal blood flow. In addition, HBO reduced the formation of 4-HNE by 33% and 76% and increased SOD by 30% and 70% in the cortex and outer stripe region of the medulla of the ischaemic kidney, respectively.
  
  
  CONCLUSIONS
  HBO attenuates the decline in GFR following renal ischaemia, and improves endothelial-dependent vasorelaxation, suggesting that treatment with HBO may be beneficial in the setting of ischaemic ARF.
authors.affiliation: Department of Physiology, the B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology and Rambam Medical Center, Haifa, 31096, Israel.
authors.forename: Irit
authors.initials: I
authors.lastname: Rubinstein
authors.forename: Zaid
authors.initials: Z
authors.lastname: Abassi
authors.forename: Felix
authors.initials: F
authors.lastname: Milman
authors.forename: Elena
authors.initials: E
authors.lastname: Ovcharenko
authors.forename: Rymond
authors.initials: R
authors.lastname: Coleman
authors.forename: Joseph
authors.initials: J
authors.lastname: Winaver
authors.forename: Ori S
authors.initials: OS
authors.lastname: Better
mesh_terms: D058186:Acute Kidney Injury / Q000503:physiopathology / Q000628:therapy; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004730:Endothelium, Vascular / Q000503:physiopathology; D005919:Glomerular Filtration Rate; D006931:Hyperbaric Oxygenation; D007511:Ischemia / Q000378:metabolism / Q000503:physiopathology; D007668:Kidney / Q000098:blood supply* / Q000293:injuries* / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D016877:Oxidants / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000503:physiopathology / Q000628:therapy*; D013482:Superoxide Dismutase / Q000378:metabolism; D014664:Vasodilation
chemical_list: D000975:Antioxidants; D016877:Oxidants; D013482:Superoxide Dismutase
references.citation: J Bone Joint Surg Am. 1986 Oct;68(8):1218-24
references.pmid: 3021776
references.citation: Kidney Int. 1995 Mar;47(3):927-34
references.pmid: 7752594
references.citation: Cardiovasc Res. 2006 Oct 1;72(1):143-51
references.pmid: 16930572
references.citation: Eur J Appl Physiol. 2001 Nov;86(1):24-7
references.pmid: 11820317
references.citation: Br J Pharmacol. 1998 Oct;125(4):817-25
references.pmid: 9831920
references.citation: J Cereb Blood Flow Metab. 2003 Oct;23(10):1219-26
references.pmid: 14526232
references.citation: Acta Neurol Scand. 1986 Aug;74(2):156-60
references.pmid: 3776462
references.citation: QJM. 2004 Jul;97(7):385-95
references.pmid: 15208426
references.citation: Neurochem Int. 2004 Jun;44(8):585-94
references.pmid: 15016473
references.citation: Gut. 1998 Oct;43(4):512-8
references.pmid: 9824579
references.citation: Kidney Int. 2004 May;65(5):1714-23
references.pmid: 15086910
references.citation: Am J Physiol Renal Physiol. 2003 Mar;284(3):F532-7
references.pmid: 12556364
references.citation: Klin Wochenschr. 1955 Aug 1;33(29-30):729-30
references.pmid: 13264515
references.citation: Nephron Exp Nephrol. 2006;104(1):e15-22
references.pmid: 16699289
references.citation: J Am Soc Nephrol. 2006 Jun;17(6):1503-20
references.pmid: 16707563
references.citation: Acta Neurochir Suppl. 2000;76:285-90
references.pmid: 11450026
references.citation: Lancet. 2005 Jan 29-Feb 4;365(9457):417-30
references.pmid: 15680458
references.citation: N Engl J Med. 1996 Jun 20;334(25):1642-8
references.pmid: 8628361
references.citation: Nephron. 1998 Dec;80(4):458-67
references.pmid: 9832646
references.citation: Am J Physiol Renal Physiol. 2001 Nov;281(5):F948-57
references.pmid: 11592952
references.citation: Surg Today. 2005;35(8):653-61
references.pmid: 16034546
references.citation: J Am Soc Nephrol. 2003 Aug;14(8):2199-210
references.pmid: 12874476
references.citation: J Bone Joint Surg Br. 2005 Apr;87(4):450-3
references.pmid: 15795190
references.citation: Semin Nephrol. 2004 Jul;24(4):316-23
references.pmid: 15252771
references.citation: Nat Clin Pract Nephrol. 2006 Jul;2(7):364-77
references.pmid: 16932465
references.citation: Aviat Space Environ Med. 1979 Mar;50(3):256-63
references.pmid: 454325
references.citation: Hypertension. 1998 Oct;32(4):746-52
references.pmid: 9774374
references.citation: Basic Clin Pharmacol Toxicol. 2006 Feb;98(2):150-4
references.pmid: 16445587
references.citation: Am J Physiol Regul Integr Comp Physiol. 2002 Feb;282(2):R335-42
references.pmid: 11792641
references.citation: Plast Reconstr Surg. 1993 May;91(6):1110-23
references.pmid: 8479978
references.citation: Curr Opin Pediatr. 2005 Apr;17(2):193-9
references.pmid: 15800411
references.citation: Ren Fail. 2002 Nov;24(6):735-46
references.pmid: 12472196
references.citation: Med Hypotheses. 1999 Mar;52(3):259-63
references.pmid: 10362286
references.citation: Physiol Res. 2003;52(1):111-6
references.pmid: 12625815
references.citation: Am J Physiol Heart Circ Physiol. 2003 Apr;284(4):H1230-9
references.pmid: 12505879
references.citation: Biochim Biophys Acta. 1983 Jul 12;752(2):233-9
references.pmid: 6860699
references.citation: Biochim Biophys Acta. 1985 Sep 25;819(1):29-36
references.pmid: 3840035
references.citation: J Neurophysiol. 2000 Apr;83(4):2022-9
references.pmid: 10758112
references.citation: Circulation. 1997 Nov 18;96(10):3655-64
references.pmid: 9396468
references.citation: Urol Int. 2007;78(1):82-5
references.pmid: 17192739