title: [Immune response induced by vaccination with pseudotyped rAAV1 expressing HPV16 L1 protein].
abstract: To investigate the feasibility of using recombinant adeno-associated virus type 1 vector as prophylactic vaccine against HPV16 infection, rAAV1-mod. HPV16L1, the recombinant AAV1 vector containing codon-modified HPV16 L1 gene, was constructed. C57BL/6 mice were immunized with purified rAAV1 vector through intramuscular and intranasal inoculation routes, and the titer of neutralizing antibody was determined by neutralization assay based on HPV16 pseudovirus. The result shows that the single dose of rAAV1-mod. HPV16L1 can induce specific neutralizing antibody in serum through both inoculation routes. Compared with intranasal group, intramuscular group can induce higher titer of neutralizing antibody. Eliciting strong and prolonged neutralizing antibody in serum, the rAAV1-mod. HPV16L1 is one of promising HPV16 prophylactic vaccine candidates.
authors.affiliation: College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100022, China.
authors.forename: Yu-bai
authors.initials: YB
authors.lastname: Zhou
authors.forename: Ze-lin
authors.initials: ZL
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Wang
authors.initials: W
authors.lastname: Sheng
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Ma
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D000229:Dependovirus / Q000235:genetics*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology*; D053918:Papillomavirus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D009856:Oncogene Proteins, Viral; D053918:Papillomavirus Vaccines; D014614:Vaccines, Synthetic; C063317:L1 protein, Human papillomavirus type 16