title: ATP-binding cassette transporters ABCA1, ABCA7, and ABCG1 in mouse spermatozoa.
abstract: Mammalian spermatozoa lose plasma membrane cholesterol during their maturation in the epididymis and during their capacitation in the female reproductive tract. While acceptors such as high-density lipoproteins (HDL) and apolipoproteins A-I (apoA-I) and J have been found in male and female reproductive tracts, transporters that mediate cholesterol efflux from plasma membranes of spermatozoa to such acceptors have not yet been defined. Candidate transporters are members of the ATP-binding cassette (ABC) transporter superfamily including ABCA1, ABCA7, ABCG1 and ABCG4, which have all been implicated in the transport of sterols and phospholipids to apolipoproteins and HDL. Here we show that mouse spermatozoa in the seminiferous tubules and epididymis express ABCA1, ABCA7 and ABCG1, but not ABCG4. Moreover, we show that ABCA1, ABCA7, and ABCG1 antibodies decrease cholesterol efflux from spermatozoa to lipid acceptors apoA-I and albumin and inhibit in vitro fertilization.
authors.affiliation: Department of Anatomy and Cell Biology, McGill University, Montreal, Que., Canada H3A2B2.
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Morales
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Marat
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ni
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Richard
authors.initials: R
authors.lastname: Oko
authors.forename: Brian T
authors.initials: BT
authors.lastname: Smith
authors.forename: W Scott
authors.initials: WS
authors.lastname: Argraves
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D000070996:ATP Binding Cassette Transporter, Subfamily G; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000418:Albumins / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D016632:Apolipoprotein A-I / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism*; D004822:Epididymis / Q000166:cytology / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D008074:Lipoproteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism
chemical_list: C487540:ABCG1 protein, mouse; D064286:ATP Binding Cassette Transporter 1; D000070996:ATP Binding Cassette Transporter, Subfamily G; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; C485856:Abca7 protein, mouse; C486685:Abcg4 protein, mouse; D000418:Albumins; D000906:Antibodies; D016632:Apolipoprotein A-I; D008074:Lipoproteins; D002784:Cholesterol
references.citation: Biochem Biophys Res Commun. 1979 May 28;88(2):613-8
references.pmid: 465058
references.citation: Biol Reprod. 1991 Jul;45(1):195-207
references.pmid: 1878433
references.citation: J Androl. 1997 Jan-Feb;18(1):32-42
references.pmid: 9089066
references.citation: J Reprod Fertil. 1982 Sep;66(1):161-8
references.pmid: 7120180
references.citation: Biochem J. 2005 Jul 15;389(Pt 2):577-85
references.pmid: 15810880
references.citation: J Lipid Res. 2004 Jun;45(6):1040-50
references.pmid: 15026428
references.citation: Biol Reprod. 1991 Jun;44(6):1113-31
references.pmid: 1873386
references.citation: Biol Reprod. 1984 Dec;31(5):1087-101
references.pmid: 6394059
references.citation: Arterioscler Thromb Vasc Biol. 1997 Aug;17(8):1605-13
references.pmid: 9301642
references.citation: Mol Reprod Dev. 2004 Dec;69(4):419-27
references.pmid: 15457546
references.citation: Biol Reprod. 1988 Feb;38(1):235-44
references.pmid: 3365471
references.citation: Mol Reprod Dev. 2003 Feb;64(2):129-35
references.pmid: 12506344
references.citation: J Lipid Res. 2006 Nov;47(11):2433-43
references.pmid: 16902247
references.citation: J Biol Chem. 1990 Aug 5;265(22):13240-7
references.pmid: 2376594
references.citation: Biochem Biophys Res Commun. 2003 Nov 14;311(2):313-8
references.pmid: 14592415
references.citation: Gamete Res. 1988 Jun;20(2):145-57
references.pmid: 3235032
references.citation: J Cell Biol. 1986 Apr;102(4):1372-7
references.pmid: 3958052
references.citation: J Biol Chem. 1999 Jan 29;274(5):3235-42
references.pmid: 9915865
references.citation: Biol Reprod. 1996 Sep;55(3):676-83
references.pmid: 8862787
references.citation: Nat Genet. 1999 Aug;22(4):347-51
references.pmid: 10431237
references.citation: Mol Reprod Dev. 1990 Feb;25(2):195-204
references.pmid: 2310569
references.citation: Nat Genet. 1999 Aug;22(4):336-45
references.pmid: 10431236