title: Patterns of cellular gene expression in swine macrophages infected with highly virulent classical swine fever virus strain Brescia.
abstract: Experimental exposure of swine to highly virulent classical swine fever virus (CSFV) strain Brescia causes an invariably fatal disease of all infected animals by 8-14 days post-infection. Host mechanisms involved in this severe outcome of infection have not been clearly established. To understand these mechanisms, we analyzed the response of primary cultured swine macrophages, a CSFV primary target cell, to infection with Brescia strain. Steady state levels of mRNA accumulation were assessed for 58 genes involved in modulation of the host immune response, at 24 and 48 h post-infection (hpi), by means of quantitative reverse transcription real-time PCR analysis (qrt-PCR). Eighteen genes showed altered expression upon infection with CSFV strain Brescia including: cytokines (IL-1alpha, IL-1beta, IL-6, and IL-12p35); cytokine receptors (IL-2Ralpha, IL-12Rbeta, and TGF-betaIIIR); chemokines (IL-8, AMCF-1, AMCF-2, MCP-2, and RANTES); interferons (INFalpha and INFbeta); and toll-like receptors (TLR3, TLR5, TLR9, and TLR10). Although these genes are associated with mechanisms of innate immune response and antiviral activity, their altered expression does not curtail CSFV Brescia growth kinetics and virus yield in swine macrophages. Data gathered here suggests that the observed gene expression profile might explain immunological and pathological changes associated with virulent CSFV infections.
authors.affiliation: Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, United States.
authors.forename: Manuel V
authors.initials: MV
authors.lastname: Borca
authors.forename: Ingigerdur
authors.initials: I
authors.lastname: Gudmundsdottir
authors.forename: Ignacio J
authors.initials: IJ
authors.lastname: Fernández-Sainz
authors.forename: Lauren G
authors.initials: LG
authors.lastname: Holinka
authors.forename: Guillermo R
authors.initials: GR
authors.lastname: Risatti
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006691:Classical Swine Fever / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000472:pathogenicity* / Q000502:physiology; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D008264:Macrophages / Q000276:immunology / Q000821:virology*; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism; D013552:Swine; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology; D014774:Virulence
chemical_list: D016207:Cytokines; D018121:Receptors, Cytokine; D051197:Toll-Like Receptor 4