title: CYP17 polymorphism and hot flushes in postmenopausal women.
abstract: OBJECTIVE
  To investigate the association between the CYP17alpha gene polymorphism and hot flushes in postmenopausal women.
  
  
  METHODS
  Ninety-three non-hysterectomized, postmenopausal women were enrolled in this study. Vasomotor symptoms were assessed at the baseline visit and based on information provided by each participant. The genotypic polymorphism of CYP17alpha gene was analyzed by PCR-RFLP assay using genomic DNA isolated from peripheral blood lymphocytes.
  
  
  RESULTS
  Thirty-six women reported hot flushes of mild intensity, 25 reported hot flushes of moderate intensity and 32 of severe intensity. There was no significant difference between the severity of hot flushes and the CYP17 genotype or allele frequencies, 0.58 and 0.67 respectively. No association was found between hot flush severity and the CYP17 allele (odds ratio = 1.17, p = 0.61).
  
  
  CONCLUSIONS
  The results of this study suggest that the CYP17 MspAI polymorphism was not significantly associated with an increased risk of reporting hot flushes.
authors.affiliation: Federal University of Sao Paulo, Sao Paulo.
authors.forename: A M
authors.initials: AM
authors.lastname: Massad-Costa
authors.forename: N C
authors.initials: NC
authors.lastname: Nogueira-de-Souza
authors.forename: C V
authors.initials: CV
authors.lastname: de Carvalho
authors.forename: I D
authors.initials: ID
authors.lastname: da Silva
authors.forename: C
authors.initials: C
authors.lastname: Guindalini
authors.forename: R C
authors.initials: RC
authors.lastname: Nogueira
authors.forename: J M
authors.initials: JM
authors.lastname: Soares
authors.forename: M A
authors.initials: MA
authors.lastname: Haidar
authors.forename: E C
authors.initials: EC
authors.lastname: Baracat
mesh_terms: D005260:Female; D005787:Gene Frequency; D005838:Genotype; D019584:Hot Flashes / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017698:Postmenopause; D012720:Severity of Illness Index; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics*
chemical_list: D013254:Steroid 17-alpha-Hydroxylase