title: Advanced preparative techniques to establish probes for molecular cytogenetics.
abstract: The methods covered in this unit include flow cytometry of metaphase chromosomes, chromosome dissection, and the DOP-PCR amplification methods for reverse chromosome painting. Successful application in these areas requires care and attention to methodological details, and this unit is particularly comprehensive.
authors.affiliation: University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Stap
authors.forename: J A
authors.initials: JA
authors.lastname: Aten
authors.forename: D
authors.initials: D
authors.lastname: Lillington
authors.forename: A
authors.initials: A
authors.lastname: Shelling
authors.forename: B D
authors.initials: BD
authors.lastname: Young
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure; D003582:Cytogenetics / Q000379:methods*; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008677:Metaphase; D015345:Oligonucleotide Probes / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes