The HIV-2 TAR RNA domain as a potential source of viral-encoded miRNA. A reconnaissance study.
Recently, it has been reported that HIV-1 TAR RNA element releases functionally competent miRNAs upon processing by Dicer enzyme. Here, we extend the analysis of miRNA viral-encoding potential to the TAR RNA of the HIV-2. Using in vitro Dicer cleavages and computer-aided analysis we have found that the 124-mer TAR RNA domain, present at the 5' end of HIV-2 mRNAs, putatively encodes pre-miRNAs. When deduced sequences of the viral-encoded miRNAs were matched against the database of human mRNA 3'-UTRs, it appeared that two miRNA candidates may target a large number of cellular transcripts.
Purzycka
Katarzyna J
KJ
Laboratory of Structural Chemistry of Nucleic Acids, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.
Adamiak
Ryszard W
RW
D001483:Base Sequence; D016325:HIV Long Terminal Repeat; D015498:HIV-2 / Q000235:genetics*; D035683:MicroRNAs / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D034622:RNA Interference; D012367:RNA, Viral / Q000737:chemistry*; D043244:Ribonuclease III / Q000378:metabolism
D035683:MicroRNAs; D012367:RNA, Viral; D043244:Ribonuclease III