Differential mass spectrometry of rat plasma reveals proteins that are responsive to 17beta-estradiol and a selective estrogen receptor modulator PPT.
Estrogens are a class of steroid hormones that interact with two related but distinct nuclear receptors, estrogen receptor (ER) alpha and beta. To identify potential ER biomarkers, we profiled the rat plasma glycoproteome after treatment with vehicle or 17beta-estradiol (E2) or an ERalpha-selective agonist PPT by differential mass spectrometry. Our comparative proteomic experiment identifies novel E2- and PPT-responsive proteins, such as serine protease inhibitor family members.
Zhao
Xuemei
X
Department of Proteomics, Merck Research Laboratories, Rahway, New Jersey 07065, USA.
Deyanova
Ekaterina G
EG
Lubbers
Laura S
LS
Zafian
Pete
P
Li
Jenny J
JJ
Liaw
Andy
A
Song
Qinghua
Q
Du
Yi
Y
Settlage
Robert E
RE
Hickey
Gerry J
GJ
Yates
Nathan A
NA
Hendrickson
Ronald C
RC
D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D009929:Organ Size; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D010949:Plasma / Q000737:chemistry*; D011720:Pyrazoles / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000819:agonists / Q000378:metabolism; D020845:Selective Estrogen Receptor Modulators / Q000378:metabolism*; D014599:Uterus / Q000033:anatomy & histology
D001798:Blood Proteins; D006023:Glycoproteins; D010455:Peptides; D010636:Phenols; D011720:Pyrazoles; D011960:Receptors, Estrogen; D020845:Selective Estrogen Receptor Modulators; C486184:4,4',4''-(4-propyl-((1)H)-pyrazole-1,3,5-triyl) tris-phenol; D004958:Estradiol