Cardiac commitment of primate embryonic stem cells.
Primate nonhuman and human embryonic stem (ES) cells provide a powerful model of early cardiogenesis. Furthermore, engineering of cardiac progenitors or cardiomyocytes from ES cells offers a tool for drug screening in toxicology or to search for molecules to improve and scale up the process of cardiac differentiation using high-throughput screening technology, as well as a source of cell therapy of heart failure. Spontaneous differentiation of ES cells into cardiomyocytes is, however, limited. Herein, we describe a simple protocol to commit both rhesus and human ES cells toward a cardiac lineage and to sort out early cardiac progenitors. Primate ES cells are challenged for 4 d with the cardiogenic morphogen bone morphogenetic protein 2 (BMP2) and sorted out using anti-SSEA-1 antibody-conjugated magnetic beads. Cardiac progenitor cells can be generated and isolated in 4 d using this protocol.
Leschik
Julia
J
INSERM-Evry University UMR861, 5 Rue Desbruères, 91030 Evry, France.
Stefanovic
Sonia
S
Brinon
Benjamin
B
Pucéat
Michel
M
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology*; D006321:Heart / Q000196:embryology*; D006801:Humans; D008253:Macaca mulatta; D023822:Tissue Engineering / Q000379:methods*
D055396:Bone Morphogenetic Protein 2