title: Cell-free protein synthesis: applications in proteomics and biotechnology.
abstract: Protein production is one of the key steps in biotechnology and functional proteomics. Expression of proteins in heterologous hosts (such as in E. coli) is generally lengthy and costly. Cell-free protein synthesis is thus emerging as an attractive alternative. In addition to the simplicity and speed for protein production, cell-free expression allows generation of functional proteins that are difficult to produce by in vivo systems. Recent exploitation of cell-free systems enables novel development of technologies for rapid discovery of proteins with desirable properties from very large libraries. This article reviews the recent development in cell-free systems and their application in the large scale protein analysis.
authors.affiliation: The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK. mingyue.he@bbsrc.ac.uk
authors.forename: Mingyue
authors.initials: M
authors.lastname: He
mesh_terms: D001709:Biotechnology / Q000639:trends*; D002474:Cell-Free System; D015202:Protein Engineering / Q000639:trends*; D040901:Proteomics / Q000639:trends*