Isoenergetic microarray mapping--the advantages of this method in studying the structure of Saccharomyces cerevisiae tRNAPhe.
Isoenergetic microarrays were applied to study the structure of processed S. cerevisiae tRNA(Phe) and its unmodified transcript. Results of hybridization experiments demonstrate significant differences in binding of both RNAs. The microarray mapping approach provides similar structural information as traditional chemical or enzymatic mapping, but is superior in several ways.
Jenek
Michal
M
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
Kierzek
Elzbieta
E
D001483:Base Sequence; D001665:Binding Sites; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D012322:RNA Precursors / Q000737:chemistry; D012331:RNA, Fungal / Q000737:chemistry; D012360:RNA, Transfer, Phe / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D015345:Oligonucleotide Probes; D012322:RNA Precursors; D012331:RNA, Fungal; D012360:RNA, Transfer, Phe