Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of Aspergillus niger.
Chitosan and its derivatives have been used as affinity matrices for purification of lipase from Aspergillus niger NCIM 1207. Trimellitic anhydride (TMA)-crosslinked deacetylated chitin adsorbed lipase selectively, yielding approximately 5-fold purification of the crude lipase with 70% yield. Further 9-fold purification occurred on eluting through Sephacryl-100. These results suggest that chitosan derivatives can be used as inexpensive biopolymer matrices for the purification of lipases for industrial applications.
Trimukhe
K D
KD
Polymer Science and Engineering Division, National Chemical Laboratory, Pune 411008, India.
Mahadik
N D
ND
Gokhale
D V
DV
Varma
A J
AJ
D000107:Acetylation; D000327:Adsorption; D001234:Aspergillus niger / Q000201:enzymology*; D048271:Chitosan / Q000031:analogs & derivatives; D003432:Cross-Linking Reagents / Q000493:pharmacokinetics; D004777:Environment; D008049:Lipase / Q000302:isolation & purification* / Q000493:pharmacokinetics*
D003432:Cross-Linking Reagents; D048271:Chitosan; D008049:Lipase