A possible extended family of regulators of sigma factor activity in Streptomyces coelicolor.
SCO4677 is one of a large number of similar genes in Streptomyces coelicolor that encode proteins with an HATPase_c domain resembling that of anti-sigma factors such as SpoIIAB of Bacillus subtilis. However, SCO4677 is not located close to genes likely to encode a cognate sigma or anti-anti-sigma factor. SCO4677 was found to regulate antibiotic production and morphological differentiation, both of which were significantly enhanced by the deletion of SCO4677. Through protein-protein interaction screening of candidate sigma factor partners using the yeast two-hybrid system, SCO4677 protein was found to interact with the developmentally specific sigma(F), suggesting that it is an antagonistic regulator of sigma(F). Two other proteins, encoded by SCO0781 and SCO0869, were found to interact with the SCO4677 anti-sigma(F) during a subsequent global yeast two-hybrid screen, and the SCO0869-SCO4677 protein-protein interaction was confirmed by coimmunoprecipitation. The SCO0781 and SCO0869 proteins resemble well-known anti-anti-sigma factors such as SpoIIAA of B. subtilis. It appears that streptomycetes may possess an extraordinary abundance of anti-sigma factors, some of which may influence diverse processes through interactions with multiple partners: a novel feature for such regulatory proteins.
Kim
Eun Sook
ES
School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
Song
Ju Yeon
JY
Kim
Dae Wi
DW
Chater
Keith F
KF
Lee
Kye Joon
KJ
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005816:Genetic Complementation Test; D047468:Immunoprecipitation; D009154:Mutation; D011485:Protein Binding; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D001426:Bacterial Proteins; D012808:Sigma Factor
Mol Microbiol. 1995 Jul;17(1):37-48
7476207
Cell Mol Biol (Noisy-le-grand). 1995 Nov;41(7):883-905
8595368
Genetics. 1996 Dec;144(4):1425-36
8978031
Mol Microbiol. 2003 Jun;48(5):1289-303
12787356
Mol Cell Biol. 1995 Oct;15(10):5820-9
7565735
Genes Dev. 1996 Sep 15;10(18):2348-58
8824593
J Bacteriol. 2008 Feb;190(3):894-904
18065550
Annu Rev Microbiol. 1993;47:685-713
7504906
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2325-9
8460142
Appl Environ Microbiol. 2006 Nov;72(11):7132-9
16950896
BMC Genomics. 2007 Aug 02;8:261
17678549
J Bacteriol. 2000 Aug;182(16):4606-16
10913095
Mol Microbiol. 2007 May;64(3):719-37
17462019
J Bacteriol. 1998 May;180(9):2515-21
9573206
Cold Spring Harb Symp Quant Biol. 1985;50:643-50
3938367
Mol Microbiol. 2003 Oct;50(2):475-86
14617172
Genes Dev. 1994 Nov 1;8(21):2653-63
7958923
Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1541-6
12563033
Cell. 1989 Oct 6;59(1):133-43
2507166
EMBO J. 1999 Aug 2;18(15):4292-8
10428967
J Mol Biol. 1996 Jul 12;260(2):147-64
8764397
J Bacteriol. 2001 Oct;183(20):5991-6
11566999
J Bacteriol. 2002 Oct;184(20):5583-9
12270815
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2330-4
8460143
Mol Microbiol. 2005 Sep;57(5):1252-64
16101999
J Mol Biol. 1983 Jun 5;166(4):557-80
6345791
Mol Microbiol. 2004 Jul;53(2):555-72
15228534
J Bacteriol. 1995 May;177(10):2912-3
7751305
J Bacteriol. 1994 Apr;176(7):1813-20
8144446
Mol Microbiol. 1996 Aug;21(3):593-603
8866481
FEMS Microbiol Lett. 2002 Apr 9;209(2):229-35
12007810
J Bacteriol. 2003 Dec;185(24):7291-6
14645292
Nature. 2002 May 9;417(6885):141-7
12000953
Mol Microbiol. 1996 Feb;19(4):901-7
8820658
J Mol Microbiol Biotechnol. 2002 Jul;4(4):427-52
12125823
Proc Natl Acad Sci U S A. 1990 Dec;87(23):9221-5
2123551
Arch Microbiol. 2001 Dec;177(1):98-106
11797050
J Biol Chem. 2007 Oct 26;282(43):31812-20
17766240
Mol Microbiol. 2001 May;40(4):804-14
11401688
Cell. 1993 Nov 19;75(4):805-16
8242751
Gene. 1992 Jun 15;115(1-2):119-25
1612425
J Bacteriol. 2004 Dec;186(24):8490-8
15576799
J Bacteriol. 1992 May;174(9):2958-67
1569025
J Bacteriol. 1996 Jul;178(13):3846-53
8682789
Mol Microbiol. 2003 Feb;47(3):699-714
12535070
Mol Microbiol. 2001 Oct;42(1):205-14
11679079
Cell. 1993 Aug 27;74(4):735-42
8358793