title: The effects of oxidative stress induced by prolonged low-dose diesel exhaust particle exposure on the generation of allergic airway inflammation differ between BALB/c and C57BL/6 mice.
abstract: We have recently reported that airway inflammatory responses to the oxidative stress induced by prolonged low-dose diesel exhaust particle (DEP) exposure differ markedly between BALB/c and C57BL/6 mice. In the present study, the effects of genetic differences in the response to prolonged low-dose DEP exposure on the generation of ovalbumin-induced allergic airway inflammation were further explored using the same mouse strains. In BALB/c mice, eosinophils and mucous goblet cells in histopathological pulmonary specimens increased significantly after DEP exposure, and were more marked than in C57BL/6 mice. Interleukin (IL)-5 and IL-13 levels in bronchoalveolar lavage (BAL) fluid were increased significantly by DEP exposure only in BALB/c mice. The DEP-induced increases in peribronchial eosinophils and mucous goblet cells in the lung tissues, and of IL-5 and IL-13 in the BAL fluid, were significantly attenuated by the antioxidant N-acetylcysteine. Thus, the effects of prolonged low-dose DEP exposure on the generation of allergic airway inflammation differed markedly between the mouse strains. These differences may be caused by different antioxidant responses to the oxidative stress induced by DEP exposure. Our results contribute more information to the search for genetic susceptibility factors in the response to DEP, and may thus assist in the discovery of new biomarkers for DEP-related disease.
authors.affiliation: Department of Hygiene and Public Health, Nippon Medical School, Tokyo, Japan.
authors.forename: Ying-Ji
authors.initials: YJ
authors.lastname: Li
authors.forename: Hajime
authors.initials: H
authors.lastname: Takizawa
authors.forename: Arata
authors.initials: A
authors.lastname: Azuma
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kohyama
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yamauchi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kawada
authors.forename: Shoji
authors.initials: S
authors.lastname: Kudoh
authors.forename: Isamu
authors.initials: I
authors.lastname: Sugawara
mesh_terms: D000111:Acetylcysteine / Q000627:therapeutic use; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D004804:Eosinophils / Q000187:drug effects / Q000276:immunology; D005260:Female; D020397:Goblet Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D052638:Particulate Matter / Q000633:toxicity*; D012130:Respiratory Hypersensitivity / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D016207:Cytokines; D007074:Immunoglobulin G; D052638:Particulate Matter; D001335:Vehicle Emissions; D007073:Immunoglobulin E; D000111:Acetylcysteine