Mutation analysis of the base-pair connecting two functional modules in the DSL ribozyme.
The class DSL ribozyme is one of artificial RNA enzymes generated by module-based molecular design. In the structure of this ribozyme, two most important functional modules are connected by a U-A base-pair. We have examined the possible importance of this base-pair by site-directed mutation experiments using the DSL-1S ribozyme and its derivative possessing altered modular organization. The analysis indicated that the DSL-1S ribozyme preferred U-A pair at the positions whereas the derivative preferred A-U pair.
Ishikawa
Junya
J
Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 819-0395, Fukuoka, Japan.
Furuta
Hiroyuki
H
Ikawa
Yoshiya
Y
D000225:Adenine / Q000737:chemistry; D020029:Base Pairing; D001483:Base Sequence; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016337:RNA, Catalytic / Q000737:chemistry*; D014498:Uracil / Q000737:chemistry
D016337:RNA, Catalytic; D014498:Uracil; D000225:Adenine