title: Response regulator YycF essential for bacterial growth: X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif.
abstract: A response regulator YycF and its cognate sensor kinase YycG constitute the two-component signal transduction system essential for growth of Gram-positive bacteria with a low GC content. We have determined the X-ray crystal structure of the effector domain of Bacillus subtilis YycF involved in DNA binding. The structure, containing a winged helix-turn-helix motif, was found to be very similar to that of the response regulator PhoB from Escherichia coli. Specific binding of YycF to the PhoB-regulated alkaline phosphatase promoter was also demonstrated.
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan. tokajima@sanken.osaka-u.ac.jp
authors.forename: Toshihide
authors.initials: T
authors.lastname: Okajima
authors.forename: Akihiro
authors.initials: A
authors.lastname: Doi
authors.forename: Ario
authors.initials: A
authors.lastname: Okada
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Gotoh
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Tanizawa
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Utsumi
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D019077:Helix-Turn-Helix Motifs / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; C119939:YycF protein, Bacteria; C061278:PhoB protein, Bacteria; D004247:DNA