Substrate preferences of a lysophosphatidylcholine acyltransferase highlight its role in phospholipid remodeling.
An important enzyme involved in phospholipid turnover is the acyl-CoA: lysophosphatidylcholine acyltransferase (LPCAT). Here, we report characterization of a newly discovered human LPCAT (LPCAT3), which has distinct substrate preferences strikingly consistent with a role in phosphatidylcholine (PtdCho) remodeling and modulating fatty acid composition of PtdCho. LPCAT3 prefers lysophosphatidylcholine (lysoPtdCho) with saturated fatty acid at the sn-1 position and exhibits acyl donor preference towards linoleoyl-CoA and arachidonoyl-CoA. Furthermore, LPCAT3 is active in mediating 1-O-alkyl-sn-glycero-3-phosphocholine acylation with long chain fatty acyl-CoAs to generate 1-O-alkyl-phosphatidylcholine, another very important constitute of mammalian membrane systems. These properties are precisely the known attributes of LPCAT previously ascribed to the isoform involved in Lands' cycle, and thus strongly suggest that LPCAT3 is involved in phospholipids remodeling to achieve appropriate membrane lipid fatty acid composition.
Kazachkov
Michael
M
Plant Biotechnology Institute, National Research Council Canada, 110 Gymnasium Place, Saskatoon, SK, S7N OW9, Canada. Michael.Kazachkov@nrc-cnrc.gc.ca
Chen
Qilin
Q
Wang
Liping
L
Zou
Jitao
J
D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000235:genetics / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D010713:Phosphatidylcholines / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D013379:Substrate Specificity
D000214:Acyl Coenzyme A; D005227:Fatty Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; C026563:arachidonyl-coenzyme A; C026562:linoleoyl-coenzyme A; D008243:1-Acylglycerophosphocholine O-Acyltransferase; C543069:LPCAT3 protein, human; C000600794:LPCAT3 protein, mouse
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