Simple and safe method for simultaneous isolation of microbial RNA and DNA from problematic populations.
We describe a novel, rapid, and safe method for extracting RNA and DNA from refractory microbes, which avoids the use of phenol or chloroform. It has been used successfully to isolate high-quality nucleic acids from pure cultures and environmental populations known to resist widely used extraction protocols.
McIlroy
Simon
S
Biotechnology Research Centre, La Trobe University, Bendigo, Victoria, Australia.
Porter
Kate
K
Seviour
Robert J
RJ
Tillett
Daniel
D
D004269:DNA, Bacterial / Q000302:isolation & purification*; D004783:Environmental Microbiology; D008967:Molecular Biology / Q000379:methods*; D012329:RNA, Bacterial / Q000302:isolation & purification*
D004269:DNA, Bacterial; D012329:RNA, Bacterial
Appl Environ Microbiol. 2000 Dec;66(12):5488-91
11097934
Microbiology (Reading). 2006 Feb;152(Pt 2):419-429
16436430
Appl Environ Microbiol. 2007 Apr;73(7):2257-70
17293509
Appl Environ Microbiol. 2000 Mar;66(3):1175-82
10698788
Syst Appl Microbiol. 1999 Sep;22(3):434-44
10553296
Syst Appl Microbiol. 2002 Apr;25(1):84-99
12086193
Microbiology (Reading). 2004 Nov;150(Pt 11):3741-3748
15528660
Appl Environ Microbiol. 1997 Nov;63(11):4223-31
9361408
Appl Environ Microbiol. 1993 Mar;59(3):695-700
7683183
FEMS Microbiol Rev. 1997 Nov;21(3):213-29
9451814
Microbiol Rev. 1995 Mar;59(1):143-69
7535888
Anal Biochem. 1983 Aug;133(1):79-84
6356981
Appl Environ Microbiol. 2002 Jun;68(6):2763-9
12039731