[Two-dimensional electrophoresis analysis of the protein expression of dental pulp in different conditions].
OBJECTIVE
To analyze the different responses of dental pulp to moderate caries and thermal stimulation by proteomics.


METHODS
Two-dimensional electrophoresis(2-DE) was performed to obtain the 2-D gel electrophoresis patterns of dental pulp. Mass spectrometry (MS) was used to analyze several different selected spots in the expression proteins.


RESULTS
No significant difference in protein expression was found between normal and moderate carious dental. Two protein spots were absent in heat-damaged group and 8 spots showed significantly down-regulated. Seven proteins were identified by MS.


CONCLUSIONS
In the present study, no significant difference in the pulp protein expression was detected between the healthy and moderate carious pulp tissues. However, down-regulation of pulp protein in thermal stimulation was observed.
Lin
Chen
C
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Nie
Min
M
Zhang
Lu
L
Chen
Zhi
Z
D000328:Adult; D003731:Dental Caries / Q000378:metabolism; D003782:Dental Pulp / Q000378:metabolism*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D020543:Proteome / Q000032:analysis / Q000096:biosynthesis*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome