Novel base-functionalized DNA. Efficient methodology for construction and bioanalytical applications.
A novel efficient two-step methodology for the construction of base-functionalized DNA is based on direct aqueous cross-coupling reactions of unprotected nucleoside triphosphates followed by polymerase incorporation. Preliminary applications of the modified DNA in electrochemical detection and bioanalysis are outlined.
Hocek
Michal
M
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, CZ-16610 Prague 6, Czech Republic. hocek@uochb.cas.cz
Vrábel
Milan
M
Cahová
Hana
H
Riedl
Jan
J
Kalachová
Lubica
L
Pivonková
Hana
H
Horáková
Petra
P
Havran
Ludek
L
Fojta
Miroslav
M
D015374:Biosensing Techniques; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D010084:Oxidation-Reduction
D003854:Deoxyribonucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase