Salmonella enterica serovar Enteritidis genes induced during oviduct colonization and egg contamination in laying hens.
Salmonella enterica serovar Enteritidis is the predominant serovar associated with salmonellosis worldwide, which is in part due to its ability to contaminate the internal contents of the hen's egg. It has been shown that S. enterica serovar Enteritidis has an unusual tropism for the avian reproductive tract and an ability to persist in the oviduct and ovary. Factors allowing S. enterica serovar Enteritidis strains to contaminate eggs could be a specific interaction with the oviduct tissue, leading to persisting oviduct colonization. In vivo expression technology, a promoter-trap strategy, was used to identify genes expressed during oviduct colonization and egg contamination with S. enterica serovar Enteritidis. A total of 25 clones with in vivo-induced promoters were isolated from the oviduct tissue and from laid eggs. Among the 25 clones, 7 were isolated from both the oviducts and the eggs. DNA sequencing of the cloned promoters revealed that genes involved in amino acid and nucleic acid metabolism, motility, cell wall integrity, and stress responses were highly expressed in the reproductive tract tissues of laying hens.
Gantois
I
I
Department of Pathology, Bacteriology and Avian Diseases, Research Group Veterinary Public Health and Zoonoses, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Ducatelle
R
R
Pasmans
F
F
Haesebrouck
F
F
Van Immerseel
F
F
D000818:Animals; D002645:Chickens / Q000382:microbiology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D010057:Oviducts / Q000382:microbiology*; D010063:Ovum / Q000382:microbiology*; D011401:Promoter Regions, Genetic; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000502:physiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial
Vaccine. 2007 Jun 15;25(25):4837-44
17485152
Mol Microbiol. 2003 Jun;48(5):1183-93
12787348
J Mol Biol. 2003 Jul 4;330(2):277-86
12823967
EMBO J. 1995 Jun 1;14(11):2551-60
7781608
Environ Microbiol. 2002 Sep;4(9):538-45
12220411
Avian Dis. 1990 Jul-Sep;34(3):721-8
2146944
EMBO J. 1996 Nov 15;15(22):6122-31
8947034
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
10829079
J Biol Chem. 2004 Nov 12;279(46):47543-54
15347649
Microbiology (Reading). 2003 Feb;149(Pt 2):525-535
12624214
Nat Rev Mol Cell Biol. 2001 May;2(5):350-6
11331909
Mol Microbiol. 1999 Oct;34(1):134-45
10540292
Int J Food Microbiol. 2006 Dec 1;112(3):253-60
16822571
Vet Rec. 1996 Mar 9;138(10):223-6
8686137
Int Microbiol. 1999 Sep;2(3):177-84
10943411
J Bacteriol. 2008 Jan;190(2):602-12
17981960
Avian Pathol. 2004 Jun;33(3):314-20
15223560
Avian Dis. 1990 Oct-Dec;34(4):991-3
2282024
J Med Microbiol. 2000 Aug;49(8):691-696
10933252
J Hyg (Lond). 1980 Jun;84(3):479-88
7052226
Mol Microbiol. 2003 Jun;48(5):1357-76
12787362
Vet Microbiol. 2005 Nov 30;111(1-2):99-105
16242866
Avian Dis. 2001 Oct-Dec;45(4):962-71
11785900
Infect Immun. 1993 Feb;61(2):504-11
8423080
J Bacteriol. 1998 Dec;180(23):6140-7
9829921
Infect Immun. 1996 Jan;64(1):61-8
8557375
Zentralbl Veterinarmed B. 1995 Oct;42(8):459-69
8578920
Appl Environ Microbiol. 2006 Feb;72(2):1055-64
16461649
Infect Immun. 2005 Jun;73(6):3358-66
15908362
Appl Environ Microbiol. 1998 Jun;64(6):2166-72
9603830
Vet Rec. 1993 Oct 16;133(16):391-3
8310606
Cell. 1999 Feb 5;96(3):341-52
10025400
Appl Environ Microbiol. 1997 Apr;63(4):1588-93
9097453
J Med Microbiol. 1999 Aug;48(8):771-780
10451001
FEMS Microbiol Rev. 1999 Jan;23(1):1-11
10077851
Vet Microbiol. 2003 May 29;93(3):223-33
12695046
Avian Dis. 2001 Jan-Mar;45(1):61-9
11332500
Nature. 1989 Apr 20;338(6217):652-5
2649795
Avian Dis. 1997 Jul-Sep;41(3):535-9
9356697
Infect Immun. 2001 Apr;69(4):2659-65
11254632
J Biol Chem. 2002 Oct 25;277(43):40309-23
12130640
Infect Immun. 2003 Dec;71(12):6734-41
14638758
Appl Environ Microbiol. 1987 Sep;53(9):2165-70
3118808
Microb Pathog. 2007 Jan;42(1):2-10
17081727
Infect Immun. 1995 Jul;63(7):2443-9
7790055
Genome Res. 2008 Oct;18(10):1624-37
18583645
J Bacteriol. 1996 Nov;178(22):6443-50
8932299
J Food Prot. 2007 Mar;70(3):623-8
17388050
Microbiol Rev. 1994 Sep;58(3):466-90
7968922
J Food Prot. 2008 Jan;71(1):19-26
18236658
Microbiology (Reading). 2004 Apr;150(Pt 4):1063-1071
15073315
Environ Microbiol. 2001 May;3(5):332-42
11422320
J Microbiol Methods. 2000 Aug;41(3):195-9
10958964
Mol Microbiol. 2000 Feb;35(4):728-42
10692151
Appl Environ Microbiol. 2005 Aug;71(8):4388-99
16085829
Gene. 1997 Mar 25;188(1):53-61
9099859
Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):934-9
9023360
J Bacteriol. 1997 Jan;179(2):317-22
8990281
Cell. 1976 Sep;9(1):91-9
788919
Clin Infect Dis. 2006 Aug 15;43(4):512-7
16838242
J Mol Biol. 2005 Jan 14;345(2):251-64
15571719
J Bacteriol. 2001 Jan;183(1):139-44
11114910