title: A two-state electronic antigen and an antibody selected to discriminate between these states.
abstract: Inspired by biology where pathways are triggered and suppressed by specific binding of two molecules, we realize a functional interface between electronics and biology by replacing one of the pair molecules with a two-state "electronic antigen" device comprising a hydroquinone monolayer assembled on gold, and choosing for the pair molecule an antibody that discriminates between the two electrically selected redox states of the monolayer. Application of an oxidative +0.6 V pulse to the antigen switches it to its benzoquinone state where antibodies bind the layer. A subsequent -0.6 V pulse reduces the monolayer back to the unbinding hydroquinone state, releases the specifically bound antibody molecules, and prevents further binding.
authors.affiliation: Department of Physics, Technion, Israel Institute of Technology, Haifa 32000, Israel.
authors.forename: Arbel
authors.initials: A
authors.lastname: Artzy-Schnirman
authors.forename: Elad
authors.initials: E
authors.lastname: Brod
authors.forename: Malki
authors.initials: M
authors.lastname: Epel
authors.forename: Monica
authors.initials: M
authors.lastname: Dines
authors.forename: Tal
authors.initials: T
authors.lastname: Hammer
authors.forename: Itai
authors.initials: I
authors.lastname: Benhar
authors.forename: Yoram
authors.initials: Y
authors.lastname: Reiter
authors.forename: Uri
authors.initials: U
authors.lastname: Sivan
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry*; D015374:Biosensing Techniques; D004563:Electrochemistry / Q000379:methods; D004581:Electronics; D004926:Escherichia coli / Q000378:metabolism; D005098:Exotoxins / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D008956:Models, Chemical; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D010100:Oxygen / Q000737:chemistry; D011549:Pseudomonas / Q000378:metabolism; D013997:Time Factors
chemical_list: D000941:Antigens; D005098:Exotoxins; D006046:Gold; D010100:Oxygen