Assays of transendothelial migration in vitro.
The inflammatory response is critical for our ability to heal wounds and fight off foreign microorganisms. Uncontrolled inflammation is also at the root of most pathologic conditions. Recruitment of leukocytes to the site of inflammation plays a defining role in the inflammatory response, and migration of leukocytes across endothelium is arguably the point of no return of the inflammatory response. Assays to study the transmigration of leukocytes have and will continue to shed light on the regulation of this vital response. Assays of transendothelial migration in vitro allow the controlled observation of this phenomenon, as well as experiments to study its regulation. In this chapter, we describe in vitro assays of transendothelial migration that have been used successfully in the authors' laboratories for decades and have proven to be reproducible, reliable, and predictive of the behavior of leukocytes and endothelial cells in models of inflammation in vivo.
Muller
William A
WA
Department of Pathology, Northwestern University, Feinberg School of Medicine, Chicago, Illinois, USA.
Luscinskas
F William
FW
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D003094:Collagen / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000166:cytology* / Q000502:physiology; D008954:Models, Biological
D003094:Collagen
Circ Res. 2007 Aug 3;101(3):234-47
17673684
J Immunol. 2007 Jan 15;178(2):1136-43
17202377
J Exp Med. 2005 Sep 19;202(6):865-76
16172263
J Exp Med. 1995 Nov 1;182(5):1337-43
7595204
Biophys J. 1994 Jun;66(6):2202-9
7521229
J Exp Med. 1997 Apr 7;185(7):1349-57
9104821
J Exp Med. 2001 Dec 17;194(12):1699-709
11748272
Circ Res. 1999 Sep 17;85(6):504-14
10488053
Immunity. 2007 Jun;26(6):784-97
17570692
Nat Immunol. 2004 Apr;5(4):393-400
15021878
J Cell Biol. 1997 Apr 21;137(2):509-19
9128259
J Exp Med. 2004 Dec 20;200(12):1571-80
15611287
Blood. 2004 Nov 15;104(10):3205-13
15280198
J Immunol. 1999 Nov 15;163(10):5640-8
10553094
Am J Pathol. 2003 May;162(5):1591-601
12707043
Cell Immunol. 2000 Jul 10;203(1):39-46
10915560
Immunity. 1999 Dec;11(6):753-61
10626897
Blood. 2007 Jun 15;109(12):5327-36
17344467
J Cell Sci. 1987 Sep;88 ( Pt 2):161-75
2961775
J Exp Med. 1992 Sep 1;176(3):819-28
1512545
J Cell Biol. 1994 Jun;125(6):1417-27
7515891
Nat Immunol. 2002 Feb;3(2):143-50
11812991
Blood. 1987 Nov;70(5):1284-90
3663936
Methods Mol Biol. 1999;96:137-45
10098131
J Exp Med. 1993 Aug 1;178(2):449-60
8340753
Science. 1998 Oct 16;282(5388):480-3
9774276
J Exp Med. 2006 Nov 27;203(12):2569-75
17116736
J Exp Med. 1989 Aug 1;170(2):399-414
2666561
BMC Immunol. 2001;2:9
11580861
Nat Immunol. 2001 Jun;2(6):515-22
11376338
Circ Res. 2006 Feb 17;98(3):394-402
16385081
Biophys J. 1992 Jul;63(1):35-57
1384734
Cell. 1991 Dec 20;67(6):1033-6
1760836
Trends Immunol. 2003 Jun;24(6):327-34
12810109
J Immunol. 2001 Aug 15;167(4):2323-30
11490021
J Exp Med. 2004 Sep 20;200(6):713-24
15381727
Microcirculation. 2001 Jun;8(3):181-93
11498781