Sesamin increases alpha-linolenic acid conversion to docosahexaenoic acid in atlantic salmon (Salmo salar L.) hepatocytes: role of altered gene expression.
In vitro cultivated Atlantic salmon (Salmo salar L.), hepatocytes were incubated without or with a mixture of sesamin and episesamin in order to test for possible effects on lipid metabolism. Sesamin/episesamin exposure (0.05 mM, final concentration) led to increased elongation and desaturation of (14)C 18:3n-3 to docosahexaenoic acid ((14)C 22:6n-3, DHA, P < 0.01) and down regulated gene expression of Delta6 and Delta5 desaturases compared to control treatment. Sesamin/episesamin further increased the hepatocytes capacity for fatty acid beta-oxidation of (14)C 18:3n-3 (P < 0.01) to the (14)C acid soluble products, acetate, malate and oxaloacetate, in agreement with an increased gene expression of carnitine palmitoyltransferase I. Also the gene expression of cluster of differentiation 36 was upregulated and the expression of scavenger receptor type B, peroxisome proliferator-activated receptors alpha and gamma were downregulated. The amount of triacylglycerols secreted by the cells tended to be lower in the sesamin/episesamin incubated hepatocytes than the control cells. This study shows that sesamin has favourable effects on lipid metabolism leading to increased level of DHA, which may be of interest for aquaculture use.
Trattner
Sofia
S
Department of Food Science, Swedish University of Agricultural Sciences, P.O. Box 7051, 75007, Uppsala, Sweden. Sofia.Trattner@lmv.slu.se
Ruyter
B
B
Østbye
T K
TK
Gjøen
T
T
Zlabek
V
V
Kamal-Eldin
A
A
Pickova
J
J
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004149:Dioxoles / Q000494:pharmacology*; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000201:enzymology / Q000378:metabolism*; D017705:Lignans / Q000494:pharmacology*; D008954:Models, Biological; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D020317:Salmo salar / Q000235:genetics / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000378:metabolism*
D000975:Antioxidants; D004149:Dioxoles; D017705:Lignans; D047493:PPAR alpha; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids; D013230:Stearoyl-CoA Desaturase; C054125:sesamin
Metabolism. 1999 Oct;48(10):1303-13
10535395
Endocrinology. 2003 Jun;144(6):2201-7
12746275
Biochem J. 1985 Apr 1;227(1):205-10
3994682
Biochim Biophys Acta. 2008 Mar;1781(3):112-22
18222184
J Biol Chem. 1957 May;226(1):497-509
13428781
Comp Biochem Physiol B. 1985;82(4):683-8
2419024
J Agric Food Chem. 2005 Dec 28;53(26):10166-78
16366711
Gene. 2000 Sep 19;255(2):411-8
11024302
J Biol Chem. 1989 Jun 25;264(18):10347-50
2732225
J Biol Chem. 1991 Oct 25;266(30):19995-20000
1834642
Methods Cell Biol. 1976;13:29-83
177845
Lipids. 2008 Nov;43(11):989-97
18781351
J Nutr Biochem. 2002 May;13(5):289-295
12015159
Alcohol Clin Exp Res. 2005 Nov;29(11 Suppl):116S-120S
16344595
Biochim Biophys Acta. 2004 Jun 1;1682(1-3):80-91
15158759
Anal Biochem. 1977 Dec;83(2):346-56
603028
Lipids. 2000 Apr;35(4):427-35
10858028
Anal Biochem. 1978 Oct 1;90(1):420-6
727482
Diabetes. 2004 Feb;53 Suppl 1:S43-50
14749265
J Chromatogr. 1988 Sep 2;448(2):249-64
3225301
Biochim Biophys Acta. 2008 Jun-Jul;1781(6-7):326-35
18503782
Lipids. 2004 Jul;39(7):649-58
15588022
Lipids. 1998 Jun;33(6):567-72
9655371
Biochim Biophys Acta. 1997 Oct 18;1348(3):331-8
9366249
J Nutr Sci Vitaminol (Tokyo). 1995 Apr;41(2):217-25
7562115
FEBS Lett. 1976 Mar 1;62(3):313-7
1278375
J Biol Chem. 1951 Nov;193(1):265-75
14907713