title: Unaltered D1, D2, D4, and D5 dopamine receptor mRNA expression and distribution in the spinal cord of the D3 receptor knockout mouse.
abstract: Dopamine (DA) acts through five receptor subtypes (D1-D5). We compared expression levels and distribution patterns of all DA mRNA receptors in the spinal cord of wild-type (WT) and loss of function D3 receptor knockout (D3KO) animals. D3 mRNA expression was increased in D3KO, but no D3 receptor protein was associated with cell membranes, supporting the previously reported lack of function. In contrast, mRNA expression levels and distribution patterns of D1, D2, D4, and D5 receptors were similar between WT and D3KO animals. We conclude that D3KO spinal neurons do not compensate for the loss of function of the D3 receptor with changes in the other DA receptor subtypes. This supports use of D3KO animals as a model to provide insight into D3 receptor dysfunction in the spinal cord.
authors.affiliation: Department of Physiology, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.forename: Stefan
authors.initials: S
authors.lastname: Clemens
authors.forename: Michael
authors.initials: M
authors.lastname: Sawchuk
authors.forename: Shawn
authors.initials: S
authors.lastname: Hochman
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000235:genetics* / Q000378:metabolism; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3 / Q000172:deficiency*; D050638:Receptors, Dopamine D4; D050641:Receptors, Dopamine D5; D013116:Spinal Cord / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D050638:Receptors, Dopamine D4; D050641:Receptors, Dopamine D5
references.citation: J Clin Invest. 1998 Aug 1;102(3):493-8
references.pmid: 9691085
references.citation: J Neurosci. 2003 Jul 16;23(15):6245-54
references.pmid: 12867509
references.citation: J Neurosci. 2002 Dec 15;22(24):10801-10
references.pmid: 12486173
references.citation: Pharmacol Ther. 1994;64(2):291-370
references.pmid: 7878079
references.citation: Cell Mol Life Sci. 2003 Jul;60(7):1267-80
references.pmid: 12943217
references.citation: Sleep Med. 2004 May;5(3):293-9
references.pmid: 15165538
references.citation: J Neurosci. 2007 Nov 28;27(48):13192-204
references.pmid: 18045913
references.citation: J Neurosci Res. 2007 Apr;85(5):1065-76
references.pmid: 17342757
references.citation: Neurology. 2006 Jul 11;67(1):125-30
references.pmid: 16832090
references.citation: Ann Med. 1998 Aug;30(4):390-6
references.pmid: 9783838
references.citation: Physiol Behav. 1997 Dec 31;63(1):137-41
references.pmid: 9402626
references.citation: Eur J Neurosci. 2004 Mar;19(5):1325-35
references.pmid: 15016090
references.citation: Eur J Neurol. 2000 May;7 Suppl 1:27-31
references.pmid: 11054156
references.citation: Genes Brain Behav. 2002 Aug;1(3):135-41
references.pmid: 12884969
references.citation: Psychopharmacology (Berl). 2005 Nov;183(1):20-30
references.pmid: 16136298
references.citation: Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11465-70
references.pmid: 15272078
references.citation: Brain Res. 1982 Jun 24;242(2):247-54
references.pmid: 6126249
references.citation: Prog Neurobiol. 2005 Oct;77(3):139-65
references.pmid: 16300874
references.citation: Eur Neuropsychopharmacol. 2008 Apr;18(4):271-7
references.pmid: 17804207
references.citation: Brain Res Bull. 2003 Jun 30;61(1):109-15
references.pmid: 12788214
references.citation: J Neurosci. 2004 Dec 15;24(50):11337-45
references.pmid: 15601940
references.citation: Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1945-9
references.pmid: 8700864
references.citation: Brain Res. 2002 Jul 26;945(1):9-19
references.pmid: 12113946
references.citation: Dev Neurosci. 1998;20(2-3):188-203
references.pmid: 9691193
references.citation: Neuroscience. 1999;91(3):911-24
references.pmid: 10391470
references.citation: Dev Biol. 1999 Dec 15;216(2):622-34
references.pmid: 10642797
references.citation: Neuropharmacology. 2002 Mar;42(3):396-413
references.pmid: 11897118
references.citation: Neurology. 1999 Mar 23;52(5):938-43
references.pmid: 10102409
references.citation: Neurology. 2000 Apr 25;54(8):1609-16
references.pmid: 10762502
references.citation: Neuroreport. 1996 Jan 31;7(2):513-6
references.pmid: 8730818
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Neuroscience. 2005;133(2):353-7
references.pmid: 15878801
references.citation: J Mol Neurosci. 2004;22(3):167-78
references.pmid: 14997010
references.citation: Physiol Rev. 1998 Jan;78(1):189-225
references.pmid: 9457173
references.citation: Neuropharmacology. 1996;35(11):1503-19
references.pmid: 9025098
references.citation: Neurosci Biobehav Rev. 1983 Summer;7(2):227-56
references.pmid: 6136014
references.citation: Sleep Med. 2007 Jun;8(4):344-8
references.pmid: 17468045
references.citation: Eur J Pharmacol. 2000 Jul 7;399(2-3):171-81
references.pmid: 10884517
references.citation: Brain Res. 2001 Mar 16;894(2):311-5
references.pmid: 11251207
references.citation: Neuroscience. 2007 Nov 23;149(4):885-97
references.pmid: 17936519
references.citation: Eur J Neurol. 2002 Nov;9 Suppl 3:59-67
references.pmid: 12464123
references.citation: Psychopharmacology (Berl). 2002 Feb;159(4):407-14
references.pmid: 11823893
references.citation: Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):2117-22
references.pmid: 15684065