title: Epizootology and experimental infection of Yokose virus in bats.
abstract: To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. YOKV belongs to the Entebbe bat virus group of vector unknown group within the genus Flavivirus and family Flaviviridae. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. Serological surveillance was conducted with samples collected in the Philippines and the sera supplied from Malaysia. One of the 36 samples from the Philippines (2.7%) and 5 of the 26 samples from Malaysia (19%) had detectable ELISA antibodies. In the experimental infections, no clinical signs of disease were observed. Moreover, no significant viral genome amplification was detected. These findings revealed that YOKV replicates poorly in the fruit bat, suggesting that fruit bats do not seem to serve as an amplifying host for YOKV.
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Shumpei
authors.initials: S
authors.lastname: Watanabe
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.forename: Mary E G
authors.initials: ME
authors.lastname: Miranda
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Masangkay
authors.forename: Naoya
authors.initials: N
authors.lastname: Ueda
authors.forename: Maiko
authors.initials: M
authors.lastname: Endo
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kato
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Tohya
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002685:Chiroptera / Q000821:virology*; D002522:Chlorocebus aethiops; D005416:Flavivirus / Q000276:immunology* / Q000302:isolation & purification; D018177:Flavivirus Infections / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D016679:Genome, Viral / Q000235:genetics / Q000276:immunology; D008296:Malaysia / Q000453:epidemiology; D010679:Philippines / Q000453:epidemiology; D011817:Rabbits; D014709:Vero Cells; D015047:Zoonoses / Q000453:epidemiology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral
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