RNA aptamers specifically interact with the Fc region of mouse immunoglobulin G.
We have designed the in vitro selection method to obtain some aptamers such as a general antibody-probing agent, which might bind to the constant regions of mouse immunoglobulin G (IgG) subclasses. As a consequence, one of the selected aptamers found to recognize mouse IgG1, 2a, and 3 subclasses. According to the binding assay, it is suggested that this aptamer recognizes the constant regions of mouse IgG subclass. In addition, this aptamer could recognize the only native form of mouse IgGs but the denatured IgGs. These features show the advantage of the aptamer as an antibody-probing agent rather than the usual secondary antibodies.
Sakai
Nobuya
N
VALWAY Technology Center, NEC Soft, Ltd., Tokyo, 136-8627, Japan.
Masuda
Hiromi
H
Akitomi
Joe
J
Yagi
Hirotaka
H
Yoshida
Yoshihito
Y
Horii
Katsunori
K
Furuichi
Makio
M
Waga
Iwao
I
D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015151:Immunoblotting; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism*; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D020349:Surface Plasmon Resonance
D052157:Aptamers, Nucleotide; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G