title: Ultrahigh-resolution spectral domain optical coherence tomography imaging of the lamina cribrosa.
abstract: Study of the structure of the lamina cribrosa is critical in glaucoma research. The purpose of this study is to determine the optimal spectral domain optical coherence tomography imaging protocol for the digital isolation and display of the lamina cribrosa. Three-dimensional datasets centered on the lamina cribrosa were obtained with 200 X 200 to 512 X 512 A-scan densities. The effect of scan density and c-mode slab thickness was subjectively compared. Increasing slab thickness reduced the sharpness of visible prelamina and lamina cribrosa structures. In retrolamina structures, thin slabs provided good visualization, but increased slab size increased the visibility of deeper structures. Scan times as short as 2.3 seconds (256 X 256 A-scans) degraded visualization of the shape of the optic nerve head. The optical scan protocol for lamina cribrosa imaging appears to be a 3 x 3 mm 200 X 200 A-scan volume with the lamina cribrosa positioned near direct current.
authors.affiliation: UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, University of Pittsburgh, Pennsylvania 15213, USA.
authors.forename: Larry
authors.initials: L
authors.lastname: Kagemann
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Gadi
authors.initials: G
authors.lastname: Wollstein
authors.forename: Peter M
authors.initials: PM
authors.lastname: Brennen
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Townsend
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Gabriele
authors.forename: Joel S
authors.initials: JS
authors.lastname: Schuman
mesh_terms: D000328:Adult; D002452:Cell Count; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009898:Optic Disk / Q000166:cytology*; D012016:Reference Values; D015203:Reproducibility of Results; D041623:Tomography, Optical Coherence / Q000379:methods*
references.citation: Arch Ophthalmol. 1981 Jan;99(1):137-43
references.pmid: 7458737
references.citation: Eye (Lond). 2004 Nov;18(11):1207-13
references.pmid: 15534606
references.citation: Trans Am Acad Ophthalmol Otolaryngol. 1974 Mar-Apr;78(2):OP290-7
references.pmid: 4825057
references.citation: Invest Ophthalmol Vis Sci. 1980 Feb;19(2):137-52
references.pmid: 6153173
references.citation: Opt Lett. 2002 Aug 15;27(16):1415-7
references.pmid: 18026464
references.citation: Opt Lett. 2007 Oct 15;32(20):2918-20
references.pmid: 17938652
references.citation: Opt Lett. 2006 Aug 15;31(16):2426-8
references.pmid: 16880844
references.citation: Opt Lett. 2000 Jan 15;25(2):111-3
references.pmid: 18059799
references.citation: Opt Lett. 2002;27(20):1803-5
references.pmid: 18033369
references.citation: Invest Ophthalmol Vis Sci. 2004 Dec;45(12):4388-99
references.pmid: 15557447
references.citation: Am J Ophthalmol. 1977 Sep;84(3):358-70
references.pmid: 900230
references.citation: Invest Ophthalmol Vis Sci. 1978 Sep;17(9):838-46
references.pmid: 81192
references.citation: Opt Lett. 2007 Jul 15;32(14):2049-51
references.pmid: 17632639
references.citation: Opt Lett. 2003 Nov 1;28(21):2067-9
references.pmid: 14587817
references.citation: Invest Ophthalmol Vis Sci. 2007 Nov;48(11):5068-84
references.pmid: 17962459
references.citation: Science. 1991 Nov 22;254(5035):1178-81
references.pmid: 1957169
references.citation: Ophthalmologica. 1964;148:431-41
references.pmid: 14265349
references.citation: Invest Ophthalmol Vis Sci. 2007 Jul;48(7):3195-208
references.pmid: 17591889
references.citation: Ophthalmologica. 1958 Aug;136(2):90-8
references.pmid: 13578338
references.citation: Prog Retin Eye Res. 2005 Jan;24(1):39-73
references.pmid: 15555526