title: [Development of a molecular beacon real-time PCR for the rapid detection of Mycobacterium tuberculosis].
abstract: OBJECTIVE
  To develop a molecular beacon real-time PCR for rapid detection of Mycobacterium tuberculosis.
  
  
  METHODS
  One set of primers was selected from the IS6110 gene in GenBank and the corresponding molecular beacon probe was designed. The specificity and sensitivity of the developed method were evaluated by tested with 10 different bacteria species. The developed assay were also applied to the diagnosis of tuberculosis.
  
  
  RESULTS
  Only Mycobacterium tuberculosis strains possessing IS6110 gene generated fluorescent signals, and no cross reaction was observed with other 9 bacteria. The detection limit was 4 copies/PCR reaction. 100 Mycobacterium tuberculosis strains were positive tested by Real-time PCR.
  
  
  CONCLUSIONS
  The established molecular beacon real-time PCR is a rapid, specific and sensitive method, and is a beneficial supplement of traditional methods for the tuberculosis diagnosis.
authors.affiliation: Department of Medical Technology, West China School of Public Health, West China Medical Center, Sichuan University, Chengdu 610041, China.
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Xu
authors.forename: Zi-ren
authors.initials: ZR
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Qu
authors.initials: Q
authors.lastname: Liu
authors.forename: Heng-chuan
authors.initials: HC
authors.lastname: Liu
mesh_terms: D015342:DNA Probes / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D015342:DNA Probes