Evaluation of rapid assays for the detection of bovine coronavirus, rotavirus A and Cryptosporidium parvum in faecal samples of calves.
Rapid immunochromatographic assays for detecting infections with bovine coronavirus (BCV), rotavirus A and Cryptosporidium parvum in calf faeces were evaluated using as gold standards a reverse transcriptase polymerase chain reaction (BCV and rotavirus) and a sedimentation-flotation technique (C. parvum). Rapid tests for the detection of BCV and rotavirus showed a high specificity (96.4% and 95.3%, respectively), but a relatively low sensitivity (60.0% and 71.9%, respectively). Sensitivity and specificity for detection of C. parvum were high (100% and 94.6%, respectively).
Klein
Daniela
D
Clinic for Ruminants, Department for Farm Animals and Herd Management, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria. Daniela.Klein@vu-wien.ac.at
Kern
Angela
A
Lapan
Gudrun
G
Benetka
Viviane
V
Möstl
Karin
K
Hassl
Andreas
A
Baumgartner
Walter
W
D000818:Animals; D000831:Animals, Newborn / Q000469:parasitology / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000821:virology; D002845:Chromatography; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary; D017938:Coronavirus, Bovine / Q000302:isolation & purification*; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000662:veterinary; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology* / Q000821:virology*; D007118:Immunoassay / Q000379:methods / Q000592:standards / Q000662:veterinary; D010270:Parasite Egg Count / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000662:veterinary; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000662:veterinary; D012680:Sensitivity and Specificity; D013997:Time Factors
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