[The value of loop-mediated isothermal amplification method for rapid diagnosis of EBV DNA].
OBJECTIVE
To establish a rapid method for EBV detection with loop-mediated isothermal amplification (LAMP), and to make it as a clue for early diagnosis of nasopharyngeal carcinoma cancer.


METHODS
EBV DNA was fast extracted from samples after boiling, while the whole detection will be finished within an hour with specific amplification of EBV gene by LAMP.


RESULTS
High specificity was shown from EBV detection of 33 clinical samples. Comparing with PCR, LAMP is more simple and convenient to perform under isothermal conditions, and require no special apparatus, thus, it is more economical and practical.


CONCLUSIONS
LAMP analysis of EBV may be an efficient and easy way for clinical diagnosis of nasopharyngeal carcinoma.
Nie
Guohui
G
Department of Otolaryngology, Second People's Hospital of Shenzhen, Shenzhen, 518035, China. nghui@21cn.com
Dong
Hongsong
H
He
Guihua
G
Xu
Xiaoping
X
Shi
Lei
L
Cao
Yicheng
Y
Chen
Xun
X
D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis*; D021141:Nucleic Acid Amplification Techniques; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004279:DNA, Viral