Collection and cryopreservation of preimplantation embryos of Cavia porcellus.
Individual differences and a rather long-lasting reproductive cycle, as well as the relatively small number of oocytes that mature during one reproductive cycle makes it difficult to establish a cryopreserved stock of preimplantation embryos of the guineapig (Cavia porcellus) when compared with other laboratory rodents. Only a few data for superovulation protocols that can be used for routine laboratory use in guineapigs are available. However, a huge number of different strains exist for many purposes and the establishment of a frozen repository makes sense. Here, we describe the successful freezing of preimplatation embryos of the strain 2BS with a two-step freezing protocol in a freezing medium containing 1,2-propanediol as cryoprotectant. Human menopausal gonodotrophin induced superovulation in the embryo donors.
Dorsch
M M
MM
Institute for Laboratory Animal Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. dorsch.martina@mh-hannover.de
Glage
S
S
Hedrich
H J
HJ
D000818:Animals; D000830:Animals, Laboratory; D001755:Blastocyst; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents; D005260:Female; D006168:Guinea Pigs / Q000502:physiology*; D008297:Male; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary; D011247:Pregnancy
D003451:Cryoprotective Agents