title: Transport of glucose by Bifidobacterium animalis subsp. lactis occurs via facilitated diffusion.
abstract: Two strains of Bifidobacterium animalis subsp. lactis were indistinguishable by several nucleic acid-based techniques; however, the type strain DSMZ 10140 was glucose utilization positive, while RB 4825, an industrially employed strain, was unable to grow rapidly on glucose as the principal carbon source. This difference was attributed to the presence of a low-affinity facilitated-diffusion glucose transporter identified in DSMZ 10140 but lacking in RB 4825. Uptake of D-[U-(14)C]glucose in DSMZ 10140 was stimulated by monovalent cations (ammonium, sodium, potassium, and lithium) and inhibited by divalent cations (calcium and magnesium). When competitor carbohydrates were included in the uptake assays, stereospecific inhibition was exhibited, with greater competition by methyl-beta-glucoside than methyl-alpha-glucoside. Significant inhibition (>30%) was observed with phloretin, an inhibitor of facilitated diffusion of glucose, whereas there was no inhibition by sodium fluoride, iodoacetate, sodium arsenate, sodium azide, 2,4-dinitrophenol, monensin, or valinomycin, which typically reduce energy-driven transport. Based on kinetic analyses, the mean values for K(t) and V(max) were 14.8 +/- 3.4 mM D-glucose and 0.13 +/- 0.03 micromol glucose/min/mg cell protein, respectively. Glucose uptake by several glucose-utilizing commercial strains of B. animalis subsp. lactis was also inhibited by phloretin, indicating the presence of facilitated diffusion glucose transporters in those strains. Since DSMZ 10140 has been previously reported to lack a functional glucose phosphoenolpyruvate phosphotransferase system, the glucose transporter identified here is responsible for much of the organism's glucose uptake.
authors.affiliation: Department of Food Science, 421 Food Science Building, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors.forename: E P
authors.initials: EP
authors.lastname: Briczinski
authors.forename: A T
authors.initials: AT
authors.lastname: Phillips
authors.forename: R F
authors.initials: RF
authors.lastname: Roberts
mesh_terms: D001644:Bifidobacterium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002250:Carbon Radioisotopes / Q000378:metabolism; D002413:Cations, Divalent / Q000494:pharmacology; D002414:Cations, Monovalent / Q000494:pharmacology; D003067:Coenzymes / Q000494:pharmacology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004058:Diffusion; D016521:Electrophoresis, Gel, Pulsed-Field; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism; D010693:Phloretin / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: D002250:Carbon Radioisotopes; D002413:Cations, Divalent; D002414:Cations, Monovalent; D003067:Coenzymes; D004269:DNA, Bacterial; D004791:Enzyme Inhibitors; D051246:Glucose Transport Proteins, Facilitative; D008757:Methylglucosides; C027020:methylglucoside; D005947:Glucose; D007785:Lactose; D010693:Phloretin
references.citation: Int J Food Microbiol. 1998 Sep 8;43(3):185-93
references.pmid: 9801194
references.citation: Appl Environ Microbiol. 2002 Dec;68(12):6429-34
references.pmid: 12450873
references.citation: J Pharm Pharmacol. 1979 Mar;31(3):168-70
references.pmid: 34692
references.citation: Appl Environ Microbiol. 1998 Feb;64(2):659-64
references.pmid: 9464404
references.citation: Appl Environ Microbiol. 1985 Jan;49(1):151-7
references.pmid: 16346694
references.citation: Curr Microbiol. 1996 Oct;33(4):216-9
references.pmid: 8824165
references.citation: J Dairy Sci. 1992 May;75(5):1192-6
references.pmid: 1317893
references.citation: J Biotechnol. 2003 Sep 4;104(1-3):77-85
references.pmid: 12948631
references.citation: J Bacteriol. 2006 Feb;188(4):1260-5
references.pmid: 16452407
references.citation: Curr Microbiol. 1996 Jun;32(6):301-7
references.pmid: 8640105
references.citation: Curr Microbiol. 1997 Sep;35(3):175-9
references.pmid: 9236301
references.citation: J Microbiol Methods. 2000 Dec 15;43(2):127-32
references.pmid: 11121611
references.citation: Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14422-7
references.pmid: 12381787
references.citation: Appl Environ Microbiol. 1997 Feb;63(2):543-6
references.pmid: 9023935
references.citation: Biochemistry. 1989 Jul 25;28(15):6410-7
references.pmid: 2506926
references.citation: Lett Appl Microbiol. 2006 Mar;42(3):189-94
references.pmid: 16478503
references.citation: J Bacteriol. 1981 Apr;146(1):10-7
references.pmid: 7012112
references.citation: Int J Syst Evol Microbiol. 2002 Nov;52(Pt 6):1945-1951
references.pmid: 12508852
references.citation: J Dairy Sci. 2006 Jul;89(7):2424-7
references.pmid: 16772558
references.citation: J Biol Chem. 1968 Jun 10;243(11):3128-35
references.pmid: 4871203
references.citation: J Physiol. 1978 May;278:389-401
references.pmid: 671319
references.citation: J Gen Physiol. 1976 Apr;67(4):381-97
references.pmid: 5575
references.citation: J Mol Microbiol Biotechnol. 2007;12(1-2):9-19
references.pmid: 17183207
references.citation: Appl Environ Microbiol. 2004 Apr;70(4):1923-30
references.pmid: 15066781
references.citation: J Bacteriol. 1997 Mar;179(5):1805-8
references.pmid: 9045844
references.citation: Int J Food Microbiol. 2005 Jul 15;102(2):221-30
references.pmid: 15992621
references.citation: Folia Microbiol (Praha). 2002;47(5):477-80
references.pmid: 12503390
references.citation: J Nutr. 1995 Jun;125(6):1401-12
references.pmid: 7782892
references.citation: J Dairy Sci. 1990 Oct;73(10):2996-3012
references.pmid: 2283425
references.citation: Antonie Van Leeuwenhoek. 2007 May;91(4):351-72
references.pmid: 17072531
references.citation: J Appl Microbiol. 2001 Feb;90(2):268-78
references.pmid: 11168730
references.citation: Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1808-12
references.pmid: 10677538
references.citation: Biochim Biophys Acta. 1971 Mar 9;233(1):189-200
references.pmid: 4931395
references.citation: Arch Microbiol. 1993;160(2):144-51
references.pmid: 8216508
references.citation: J Bacteriol. 1989 Dec;171(12):6808-14
references.pmid: 2687256
references.citation: J Biol Chem. 1959 Nov;234:3022-6
references.pmid: 14415272