title: Stb1 collaborates with other regulators to modulate the G1-specific transcriptional circuit.
abstract: G(1)-specific transcription in the budding yeast Saccharomyces cerevisiae depends upon SBF and MBF. Whereas inactivation of SBF-regulated genes during the G(1)/S transition depends upon mitotic B-type cyclins, inactivation of MBF has been reported to involve multiple regulators, Nrm1 and Stb1. Nrm1 is a transcriptional corepressor that inactivates MBF-regulated transcription via negative feedback as cells exit G(1) phase. Cln/cyclin-dependent kinase (CDK)-dependent inactivation of Stb1, identified via its interaction with the histone deacetylase (HDAC) component Sin3, has also been reported to inactivate MBF-regulated transcription. This report shows that Stb1 is a stable component of both SBF and MBF that binds G(1)-specific promoters via Swi6 during G(1) phase. It is important for the growth of cells in which SBF or MBF is inactive. Although dissociation of Stb1 from promoters as cells exit G(1) correlates with Stb1 phosphorylation, phosphorylation is only partially dependent upon Cln1/2 and is not involved in transcription inactivation. Inactivation depends upon Nrm1 and Clb/CDK activity. Stb1 inactivation dampens maximal transcriptional induction during late G(1) phase and also derepresses gene expression in G(1)-phase cells prior to Cln3-dependent transcriptional activation. The repression during G(1) also depends upon Sin3. We speculate that the interaction between Stb1 and Sin3 regulates the Sin3/HDAC complex at G(1)-specific promoters.
authors.affiliation: Department of Molecular Biology, MB-3 The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Robertus A M
authors.initials: RA
authors.lastname: de Bruin
authors.forename: Tatyana I
authors.initials: TI
authors.lastname: Kalashnikova
authors.forename: Curt
authors.initials: C
authors.lastname: Wittenberg
mesh_terms: D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D016193:G1 Phase / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D006655:Histone Deacetylases; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C082719:CLN1 protein, S cerevisiae; C082721:CLN2 protein, S cerevisiae; D016213:Cyclins; C083209:MBP1 protein, S cerevisiae; C513403:Nrm1 protein, S cerevisiae; D012097:Repressor Proteins; C081540:SBF protein, S cerevisiae; C071731:SIN3 protein, S cerevisiae; C120525:STB1 protein, S cerevisiae; C075111:SWI6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D018844:Cyclin-Dependent Kinases; D006655:Histone Deacetylases
references.citation: Genes Dev. 1996 Jan 15;10(2):129-41
references.pmid: 8566747
references.citation: Genetics. 2005 Sep;171(1):49-61
references.pmid: 15965243
references.citation: Cell. 2001 Nov 16;107(4):537-48
references.pmid: 11719193
references.citation: EMBO J. 1993 May;12(5):1955-68
references.pmid: 8387915
references.citation: Science. 1993 Sep 17;261(5128):1551-7
references.pmid: 8372350
references.citation: J Cell Sci. 2004 May 1;117(Pt 11):2173-81
references.pmid: 15126619
references.citation: Oncogene. 2005 Apr 18;24(17):2796-809
references.pmid: 15838516
references.citation: Mol Cell Biol. 1996 Jun;16(6):2647-55
references.pmid: 8649372
references.citation: Nucleic Acids Res. 1996 Feb 15;24(4):558-65
references.pmid: 8604294
references.citation: Genes Dev. 1995 Nov 15;9(22):2780-94
references.pmid: 7590253
references.citation: Nat Biotechnol. 1999 Oct;17(10):1030-2
references.pmid: 10504710
references.citation: Mol Biol Cell. 2003 Aug;14(8):3230-41
references.pmid: 12925759
references.citation: Cell. 2001 Sep 21;106(6):697-708
references.pmid: 11572776
references.citation: Curr Genet. 2005 Jan;47(1):1-17
references.pmid: 15565322
references.citation: Oncogene. 2006 Aug 28;25(38):5228-32
references.pmid: 16936741
references.citation: Yeast. 1998 Jul;14(10):953-61
references.pmid: 9717241
references.citation: Cell. 1993 Sep 24;74(6):993-1007
references.pmid: 8402888
references.citation: Mol Cell. 2006 Aug;23(4):483-96
references.pmid: 16916637
references.citation: Nature. 2001 Jan 25;409(6819):533-8
references.pmid: 11206552
references.citation: Mol Cell Biol. 2003 Jul;23(14):5064-77
references.pmid: 12832490
references.citation: Anal Chem. 2001 Dec 1;73(23):5683-90
references.pmid: 11774908
references.citation: Proc Natl Acad Sci U S A. 1989 Aug;86(16):6255-9
references.pmid: 2569741
references.citation: Cell. 2004 Jun 25;117(7):899-913
references.pmid: 15210111
references.citation: Mol Cell. 2001 Jun;7(6):1213-20
references.pmid: 11430824
references.citation: Mol Biol Cell. 1998 Dec;9(12):3273-97
references.pmid: 9843569
references.citation: Mol Cell Biol. 1999 Aug;19(8):5267-78
references.pmid: 10409718
references.citation: Science. 1997 Oct 17;278(5337):455-60
references.pmid: 9334303
references.citation: EMBO J. 1995 Oct 2;14(19):4803-13
references.pmid: 7588610
references.citation: Mol Gen Genet. 1997 Oct;256(4):376-86
references.pmid: 9393435
references.citation: Cell. 2004 Jun 25;117(7):887-98
references.pmid: 15210110
references.citation: Oncogene. 2005 Apr 18;24(17):2746-55
references.pmid: 15838511