Protein-facilitated ribozyme folding and catalysis.
In vivo, large RNAs rely on proteins to fold to their native conformation. In the case of the S. cerevisiae group II intron ai5 gamma, the DEAD-box protein Mss116 has been shown to promote the formation of the catalytically active structure. However, it is a matter of debate whether it does this by stabilizing on-pathway intermediates or by disrupting misfolded structures. Here we present the available experimental evidence to distinguish between those mechanisms and discuss the possible interpretations.
Zingler
Nora
N
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA. nora.zingler@yale.edu
Solem
Amanda
A
Pyle
Anna Marie
AM
D001483:Base Sequence; D002384:Catalysis; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D016337:RNA, Catalytic / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D016337:RNA, Catalytic; D029701:Saccharomyces cerevisiae Proteins; C058150:MSS116 protein, S cerevisiae; D053487:DEAD-box RNA Helicases