title: Reagents and instruments for multiplexed analysis using microparticles.
abstract: Multiplexed molecular analysis by means of flow cytometry using optically encoded microspheres is a rapidly expanding application that has its roots in the earliest days of flow cytometry. The approach is driven by increasing demand for analytical methods to measure large numbers of biomolecules quantitatively and sensitively in small volumes of sample. Encoded microspheres and flow cytometry have been employed for a wide range of multiplexed molecular analysis, and detailed protocols for many of these have been developed. The goal of this unit is to provide an overview of the concepts, instruments, and reagents that enable these assays.
authors.affiliation: La Jolla Bioengineering Institute, La Jolla, California, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Nolan
authors.forename: Loretta
authors.initials: L
authors.lastname: Yang
authors.forename: Henri C
authors.initials: HC
authors.lastname: van der Heyde
mesh_terms: D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D002614:Chelating Agents; D004396:Coloring Agents; D005434:Flow Cytometry / Q000379:methods*; D008863:Microspheres; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D002614:Chelating Agents; D004396:Coloring Agents