title: Dual role of FtsH in regulating lipopolysaccharide biosynthesis in Escherichia coli.
abstract: In Escherichia coli, FtsH (HflB) is a membrane-bound, ATP-dependent metalloendoprotease belonging to the AAA family (ATPases associated with diverse cellular activities). FtsH has a limited spectrum of known substrates, including the transcriptional activator sigma32. FtsH is the only known E. coli protease that is essential, as it regulates the concentration of LpxC, which carries out the first committed step in the synthesis of lipid A. Here we identify a new FtsH substrate--3-deoxy-D-manno-octulosonate (KDO) transferase--which carries out the attachment of two KDO residues to the lipid A precursor (lipid IVA) to form the minimal essential structure of the lipopolysaccharide (LPS) (KDO2-lipid A). Thus, FtsH regulates the concentration of the lipid moiety of LPS (lipid A) as well as the sugar moiety (KDO-based core oligosaccharides), ensuring a balanced synthesis of LPS.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Life Sciences, Tel Aviv University, Tel Aviv, Israel 69978.
authors.forename: Chen
authors.initials: C
authors.lastname: Katz
authors.forename: Eliora Z
authors.initials: EZ
authors.lastname: Ron
mesh_terms: D049069:ATP-Dependent Proteases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008050:Lipid A / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D013400:Sugar Acids / Q000737:chemistry / Q000378:metabolism; D014166:Transferases / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D008050:Lipid A; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; D014166:Transferases; C019917:3-deoxy-D-manno-octulosonate transferase; D049069:ATP-Dependent Proteases; C513383:FtsH protein, E coli
references.citation: FEBS Lett. 1999 Nov 5;460(3):554-8
references.pmid: 10556534
references.citation: Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3516-20
references.pmid: 7724592
references.citation: Genes Dev. 1998 May 1;12(9):1348-55
references.pmid: 9573051
references.citation: Trends Biochem Sci. 2006 Dec;31(12):647-53
references.pmid: 17074491
references.citation: J Bacteriol. 2005 Jun;187(11):3807-13
references.pmid: 15901705
references.citation: J Bacteriol. 2004 Nov;186(22):7474-80
references.pmid: 15516558
references.citation: Experientia. 1992 Feb 15;48(2):178-201
references.pmid: 1740190
references.citation: J Biol Chem. 1989 Apr 25;264(12):6956-66
references.pmid: 2651435
references.citation: Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15264-9
references.pmid: 11742086
references.citation: Annu Rev Pathol. 2006;1:467-96
references.pmid: 18039123
references.citation: J Bacteriol. 1997 Jan;179(2):358-63
references.pmid: 8990286
references.citation: J Bacteriol. 1998 Mar;180(6):1573-7
references.pmid: 9515930
references.citation: Mol Microbiol. 2008 Feb;67(3):633-48
references.pmid: 18093093
references.citation: EMBO J. 1995 Jun 1;14(11):2551-60
references.pmid: 7781608
references.citation: Proc Natl Acad Sci U S A. 1995 May 9;92(10):4532-6
references.pmid: 7753838
references.citation: FEBS Lett. 2001 Mar 23;493(1):17-20
references.pmid: 11277997
references.citation: Mol Microbiol. 2006 Feb;59(3):1025-36
references.pmid: 16420369
references.citation: Annu Rev Microbiol. 2005;59:211-31
references.pmid: 15910274
references.citation: Mol Cell. 2003 Mar;11(3):659-69
references.pmid: 12667449
references.citation: Mol Microbiol. 2000 Sep;37(6):1436-43
references.pmid: 10998174
references.citation: Biochem Soc Trans. 2008 Feb;36(Pt 1):68-71
references.pmid: 18208388
references.citation: Mol Microbiol. 1999 Feb;31(3):833-44
references.pmid: 10048027
references.citation: Biochem Soc Trans. 2008 Feb;36(Pt 1):72-7
references.pmid: 18208389
references.citation: J Biol Chem. 1992 May 15;267(14):9988-97
references.pmid: 1577828
references.citation: Annu Rev Biochem. 2002;71:635-700
references.pmid: 12045108
references.citation: Microbiology (Reading). 2007 Aug;153(Pt 8):2560-2571
references.pmid: 17660420
references.citation: Arch Biochem Biophys. 2000 Aug 1;380(1):103-7
references.pmid: 10900138
references.citation: Semin Cell Biol. 1990 Dec;1(6):423-32
references.pmid: 2103893
references.citation: J Lipid Res. 2006 May;47(5):1097-111
references.pmid: 16479018
references.citation: Mol Microbiol. 1997 Jun;24(6):1303-10
references.pmid: 9218777
references.citation: J Biol Chem. 1996 Dec 6;271(49):31196-201
references.pmid: 8940120
references.citation: J Bacteriol. 1993 Dec;175(23):7720-3
references.pmid: 8244945
references.citation: J Biol Chem. 1999 Jul 30;274(31):22002-7
references.pmid: 10419524
references.citation: J Mol Biol. 2007 Sep 14;372(2):485-96
references.pmid: 17651755
references.citation: Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10861-5
references.pmid: 8248182
references.citation: Biochem Soc Trans. 2008 Feb;36(Pt 1):120-5
references.pmid: 18208398