[Possible inhibitory effect of nitric oxide donor, dipropylenetriamine nonoate (DPTA/NO), on oxygen consumption of isolated rats' testis interstitial cells].
BACKGROUND
The understanding of testicular physiology, pathology, and male fertility issues requires knowledge of male germ cell death, energy production and oxygen consumption. The aim of this study was to examine the effect of a new donor of exogenous nitric oxide, di-propylen-three amin-NONOate (DPTA/NO), on the oxygen consumption by freshly isolated rats' testis interstitial cells.


METHODS
Rats' testis interstitial cells (ISC) were isolated according to Anakwe method and oxygen consumption was measured in an ex vivo bath. The ISC were stimulated with glutamate (0.5 M, 20 microl). DPTA (10(-3) - 10(-6) M) was added and its effect was tested in the absence of ADP (state 4 of respiration) and after that in the presence of ADP (state 3). The reaction was followed for 10 minutes and for each concentration the measurement was done in duplicate. The same measurements without treatment of DPTA/NO were done for control group.


RESULTS
DPTA/NO (10(-4) - 10(-6) M) reduced the oxygen consumption rate compared with the control group. The inhibition of respiration was not noticed in concentration of 10(-3) M. In V3 respiratory phase, all tested concentrations of DPTA/NO (10(-3) - l0(-6) M) decreased the oxygen consumption rate in comparison with the control group.


CONCLUSIONS
Our results demonstrate that a NO donor, DPTA/NO (10(-3) - 10(-6) M) inhibits oxygen consumption in isolated rat testis interstitial cells in a manner that could be concentration dependent. Because of limited number of measurements this was not fully proved and additional experiments are needed.
Kojić
Zvezdana
Z
Institut za fiziologiju, Medicinski fakultet Beograd. zvezdanak@med.bg.ac.yu
Delić
Marina
M
Bosnić
Olivera
O
Sćepanović
Ljiljana
L
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000475:Alkenes / Q000494:pharmacology*; D000818:Animals; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D000475:Alkenes; D020030:Nitric Oxide Donors; C453079:dipropylenetriamine-NONOate; D000244:Adenosine Diphosphate