Zinc finger protein-based detection system of PCR products for pathogen diagnosis.
A novel detection system of PCR products from bacterial genomes using Zinc finger proteins was developed. Zinc finger proteins are DNA-binding proteins that can bind to dsDNA with high affinity and specificity. Since Zinc finger proteins can directly detect PCR products and can double-check the specific PCR amplification and sequence specificity of the PCR products, this novel method would be quick and highly accurate. In this study, we tried to construct the detection system for three pathogen, Legionella pneumophila, Salmonella spp. and Influenza A virus using well-characterized Zinc finger proteins. As a result, we succeeded in detecting the PCR products from Legionella pneumophila, Salmonella spp. and Influenza A virus using Sp1 and Zif268. Therefore, this methodology can be applied to the detection of most pathogen using various Zinc finger proteins.
Osawa
Yuko
Y
Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Motoki
Hiroaki
H
Matsuo
Takafumi
T
Horiuchi
Michio
M
Sode
Koji
K
Ikebukuro
Kazunori
K
D001419:Bacteria / Q000302:isolation & purification*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification; D016952:Legionella pneumophila / Q000235:genetics / Q000302:isolation & purification; D025202:Molecular Diagnostic Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014780:Viruses / Q000302:isolation & purification*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D016329:Sp1 Transcription Factor