title: [Construction of siRNA expression vector of NgR and its inhibition on the expression of NgR].
abstract: OBJECTIVE
  To construct the expressing vector of siRNA in order to inhibit NgR expression, which provides a new gene therapy approach to the optic nerve impairment.
  
  
  METHODS
  It was a experimental study. Specific short chain oligonucleotides was designed by using the siRNA software according to the mRNA sequence provided by genebank, the double chain DNA sequence was gained through annealing after chemosynthesis and was inserted to the pSUPER-EGFP vector linearized with Bgl II and Hind II enzyme, the recombinant expression vector was evaluated by using EcoR I and Hind II enzyme cutting and sequencing. At last, the constructed vectors were transfected into wistar rat retinal ganglion cells and the expression level of NgR was observed.
  
  
  RESULTS
  Identification by enzyme cutting and sequencing showed that the expression vector was constructed successfully, and it was proved that the NgR expression level was effectively inhibited.
  
  
  CONCLUSIONS
  The constructed siRNA expression vectors can block the NgR gene expression, it will be the foundation for the research of gene therapy to optic nerve regeneration.
authors.affiliation: Research Institute of Field Surgery, Da Ping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Chen
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Chen
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Yin
authors.forename: Jian
authors.initials: J
authors.lastname: Ye
mesh_terms: D000818:Animals; D015870:Gene Expression; D005822:Genetic Vectors; D009185:Myelin Proteins / Q000235:genetics*; D034741:RNA, Small Interfering; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000235:genetics*; D014162:Transfection
chemical_list: D009185:Myelin Proteins; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface