A novel genomic constellation (G10P[3]) of group A rotavirus detected from buffalo calves in northern India.
Group A bovine rotaviruses cause gastroenteritis and calf mortality leading to significant economic losses to dairy farmers in India. Due to segmented nature of the RNA genome and wide host range, vast genetic and antigenic diversity exists among different isolates of rotavirus. Molecular characterization of locally prevalent group A rotavirus strains in buffalo population in north India was undertaken. Out of a total of 455 faecal samples, 21 samples (4.61%) were positive for bovine rota virus (BRV) as determined by PAGE and ELISA, whereas of these only 15 isolates yielded specific products for VP4 and VP7 genes by RT-PCR. Genotyping by nested PCR typed G6, G10 and P[11] genotypes but VP4 genes of 11 isolates remained untyped. The phylogenetic and evolutionary analysis of nucleotide and predicted amino acid sequences of the cloned products of VP4 and VP7 genes confirmed typing results obtained by nested PCR for G6, G10 and P[11] and classified the untyped isolates as P[3] genotypes. In this study, it was observed that G6P[11] (26.66%) and G10P[3] (73.34%) group A rotaviruses are circulating in buffalo herds of organized farms in north India. Unusual reassortants G10P[3] of group A rotaviruses isolated from buffalo calves show novel genomic constellations indicative of interspecies reassortment.
Manuja
Balvinder K
BK
Department of Animal Biotechnology, CCS Haryana Agricultural University, Hisar (Haryana) 125004, India. bmanuja@lycos.com
Prasad
Minakshi
M
Manuja
Anju
A
Gulati
Baldev R
BR
Prasad
Gaya
G
D000818:Animals; D002020:Buffaloes / Q000821:virology*; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins