A whole base-labile strategy for RNA synthesis with 2'-O-acetalester protections.
The use of base-labile groups for 2'-OH protection is a major challenge for RNA synthesis. A new method with acyloxymethyl groups has been developed. These groups were fully compatible with standard base-labile protections for nucleobases and phosphates and were removed in a short two-step all-base deprotection. Oligoribonucleotides were synthesized efficiently, rapidly and in high purity without chain rupture or isomerisation via this new whole base-labile strategy.
Lavergne
Thomas
T
IBMM, UMR 5247 CNRS-UM1-UM2, CC 1704, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
Martin
Anthony
A
Debart
Françoise
F
Vasseur
Jean-Jacques
JJ
D001671:Biochemistry / Q000379:methods; D004952:Esters / Q000737:chemistry; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis* / Q000737:chemistry; D012263:Ribonucleosides / Q000138:chemical synthesis / Q000737:chemistry
D004952:Esters; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; D034741:RNA, Small Interfering; D012263:Ribonucleosides; C434331:phosphoramidite