title: N-(3-triethoxysilylpropyl)-4-(isothiocyanatomethyl)-cyclohexane-1-carboxamide (TPICC): a heterobifunctional reagent for immobilization of biomolecules on glass surface.
abstract: An efficient heterobifunctional reagent, N-(3-triethoxysilylpropyl)-4-(isothiocyanatomethyl)cyclohexane-1-carboxamide (TPICC) has been developed by a simple 'two step reaction' for the preparation of bioconjugates and immobilization of biomolecules such as oligonucleotides, peptides and proteins on the glass surface. The isothiocyanate functionality at one end of the reagent, TPICC was found specific for the ligands having either aminoalkyl (RNH(2)) or mercaptoalkyl (R-SH) functionality. The synthesis of bioconjugates with the reagent was achieved through its isothiocyanate functionality at one end via the formation of stable thiourea linkage with aminoalkyl and dithiocarbamate linkage with mercaptoalkyl derivatives. The triethoxysilyl functionality of the reagent has been utilized for specific coupling with the virgin glass surface by a very simple methodology.
authors.affiliation: Nucleic Acids Research Laboratory, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221 005, India. arvindmisra2003@yahoo.com
authors.forename: Arvind
authors.initials: A
authors.lastname: Misra
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shahid
authors.forename: Pratibha
authors.initials: P
authors.lastname: Dwivedi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003432:Cross-Linking Reagents; D003510:Cyclohexanes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D005453:Fluorescence; D005898:Glass / Q000737:chemistry; D007202:Indicators and Reagents; D017879:Isothiocyanates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D015394:Molecular Structure; D015345:Oligonucleotide Probes / Q000737:chemistry; D013890:Thiourea / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D003510:Cyclohexanes; D007202:Indicators and Reagents; D017879:Isothiocyanates; C532456:N-(3-triethoxysilylpropyl)-4-(isothiocyanatomethyl)cyclohexane-1-carboxamide; D015345:Oligonucleotide Probes; D013890:Thiourea