[Apoptosis-inducing effect of 5-aminolevulinic acid-mediated photodynamic therapy (5-ALA-PDT) on cervical cancer cell lines].
OBJECTIVE
5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) is a new ablation treatment for tumors, while its function mechanism in cervical cancer is unclear. This study was to investigate the effects of ALA-PDT on cervical cancer cell lines.


METHODS
The effects of ALA-PDT on proliferation of 8 human cervical cancer cell lines were examined by MTT assay to find out the optimal function parameters of ALA-PDT and the most sensitive cell line. The effect of ALA-PDT on apoptosis of cervical cancer cell line Me180 was investigated by using Annexin V-FITC/PI double staining, Hoechst 33342 staining and May-Grunwald-Farbstoff Giemsa staining. Cell cycle of Me180 cells was observed by flow cytometry with PI staining. The effect of ALA-PDT on the expression of survivin in Me180 cells was detected by Western blot and real-time fluorescence reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
Among the 8 cell lines, Me180 cell line was most sensitive to ALA-PDT; 2 mmol/L ALA, 10 J/cm(2) light dose and 3-hour treatment were the optimal function parameters of ALA-PDT, and the 50% inhibition concentration (IC(50)) of ALA-PDT under this situation for Me180 cells was 7.28x10(-4) mmol/L. ALA-PDT induced apoptosis and G0/G1 phase arrest of Me180 cells, and suppressed the mRNA and protein expression of survivin in Me180 cells.


CONCLUSIONS
ALA-PDT can inhibit the proliferation and induce apoptosis of cervical cancer cell line Me180 in vitro, which may relate with the suppression of survivin expression.
He
Gui-Fang
GF
Department of Gynecology & Obstetrics,China-Japan Friendship Hospital, Beijing, 100029, PR China. bianmeilu@hotmail.com.
Bian
Mei-Lu
ML
Zhao
Ya-Wei
YW
Xiang
Qing
Q
Li
Hong-Yan
HY
Xiao
Cheng
C
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D000077022:Survivin; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology*
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D017319:Photosensitizing Agents; D012333:RNA, Messenger; D000077022:Survivin; D000622:Aminolevulinic Acid