Effect of antiplaque compounds and mouthrinses on the activity of glucosyltransferases from Streptococcus sobrinus and insoluble glucan production.
BACKGROUND
The development of therapeutic agents inhibiting the activity of glucosyltransferases (GTF) and their production of glucans is a potential strategy to reduce dental decay. The aim of this study was first to characterize a GTF preparation from Streptococcus sobrinus ATCC 33478 and then to evaluate the effects of select compounds and mouthrinses on insoluble glucan (ISG) formation by combined GTFs.


METHODS
The purity of the crude GTF mixture was assessed by electrophoresis. The effects of pH, temperature, sucrose, and dextran T10 concentrations on GTF activity were analyzed and the chemical structure of the products was investigated. Finally, the inhibition of GTF by commercial mouthrinses used in oral hygiene and their active components (chlorhexidine, polyphenolic compounds, fluoride derivatives, polyols, cetylpyridinium chloride, and povidone iodine) was analyzed through the reductions in the overall reaction rate and the quantity of ISG synthesized.


RESULTS
The S. sobrinus ATCC 33478 crude GTF preparation obtained contains a mixture of four different GTFs known for this species. For optimal adherent ISG formation, the reaction parameters were 37 degrees C, pH 6.5, sucrose 50 g/l, and dextran T10 2 g/l. Under these conditions, the most effective agents were chlorhexidine, cetylpyridinium chloride, and tannic acid. Eludril, Elmex, and Betadine were the most effective inhibitors of all the mouthrinses tested.


CONCLUSIONS
As the formulation of commercial products considerably influences the efficiency of active components, the fast representative ISG inhibition test developed in this study should be of great interest.
Furiga
A
A
Laboratoire Odontologique de Recherche, UFR d'Odontologie, Université Victor Segalen Bordeaux 2, Bordeaux, France.
Dols-Lafargue
M
M
Heyraud
A
A
Chambat
G
G
Lonvaud-Funel
A
A
Badet
C
C
D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002327:Cariostatic Agents / Q000494:pharmacology; D002594:Cetylpyridinium / Q000494:pharmacology; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D003773:Dental Plaque / Q000517:prevention & control*; D003911:Dextrans / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D005936:Glucans / Q000037:antagonists & inhibitors* / Q000737:chemistry; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors* / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D009067:Mouthwashes / Q000494:pharmacology*; D053210:Pharmaceutical Preparations, Dental / Q000494:pharmacology*; D010636:Phenols / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology; D059808:Polyphenols; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000187:drug effects*; D011206:Povidone-Iodine / Q000494:pharmacology; D012995:Solubility; D016986:Streptococcus sobrinus / Q000187:drug effects / Q000201:enzymology*; D013395:Sucrose / Q000494:pharmacology; D013634:Tannins / Q000494:pharmacology; D013696:Temperature
D000891:Anti-Infective Agents, Local; D002327:Cariostatic Agents; D003911:Dextrans; D004791:Enzyme Inhibitors; D005419:Flavonoids; D005936:Glucans; D009067:Mouthwashes; D053210:Pharmaceutical Preparations, Dental; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D011135:Polysaccharides, Bacterial; D013634:Tannins; C024617:polyol; D013395:Sucrose; D011206:Povidone-Iodine; D002594:Cetylpyridinium; D005964:Glucosyltransferases; C010882:chlorhexidine gluconate; D005459:Fluorides; D002710:Chlorhexidine