title: [Study on determination of acetamiprid and interaction between acetamiprid and deoxyribonucleic acid by resonance light scattering].
abstract: The interaction between acetamiprid and deoxyribonucleic acid (DNA) was used to determine acetamiprid by resonance light scattering (RLS). The RLS signals of DNA were greatly enhanced by acetamiprid in the spectrum region of 300-600 nm. The spectrum peak is around 316.0 nm. The optimum conditions: pH is 1.73; the concentration of DNA is 2.0 microg x mL(-1)bration curve is 0-2. 25 pg * mLU , with the detection2limit of 0. 2 ig * mL '. The acetamiprid in river water sample was determined. The results were satisfactory, and the recovery rates were in the range of 98%-106%. The interaction mechanism of acetamiprid and DNA was discussed: the interactions between acetamiprid and nucleic acid base include electrostatic effect and Tr-r cumulate effect.
authors.affiliation: College of Science, China Agricultural University, Beijing 100094, China. dufp@cau.edu.cn
authors.forename: Feng-pei
authors.initials: FP
authors.lastname: Du
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Luo
authors.forename: Shi-cong
authors.initials: SC
authors.lastname: Hou
authors.forename: Ke-an
authors.initials: KA
authors.lastname: Li
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Ren
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
authors.forename: Zhao-shu
authors.initials: ZS
authors.lastname: Mao
authors.forename: Nian-qin
authors.initials: NQ
authors.lastname: Jie
mesh_terms: D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008027:Light; D000073943:Neonicotinoids; D011725:Pyridines / Q000032:analysis* / Q000737:chemistry; D012542:Scattering, Radiation; D055672:Static Electricity
chemical_list: D000073943:Neonicotinoids; D011725:Pyridines; C464485:acetamiprid; D004247:DNA