Lipase-catalyzed reactions at interfaces of two-phase systems and microemulsions.
This work describes the influence of two polar lipids, Sn-1/3 and Sn-2 monopalmitin, on the activity of lipase in biphasic systems and in microemulsions. In previous communications, we have shown that Sn-2 monoglycerides can replace Sn-1,3 regiospecific lipases at the oil-water interface, causing a drastically reduced rate of lipolysis. We here demonstrate that even if the lipase is expelled from the interface, it can catalyze esterification of the Sn-2 monoglyceride with fatty acids in both macroscopic oil-water systems and in microemulsions, leading to formation of di- and triglycerides.
Reis
P
P
Nestlé Research Center, 1000 Lausanne 26, Switzerland. pedro_esb@hotmail.com
Miller
R
R
Leser
M
M
Watzke
H
H
D002384:Catalysis; D004655:Emulsions; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D050178:Monoglycerides / Q000737:chemistry; D009821:Oils / Q000737:chemistry*; D044367:Phase Transition; D014867:Water / Q000737:chemistry*
D004655:Emulsions; D050178:Monoglycerides; D009821:Oils; D014867:Water; D008049:Lipase
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