title: TRPing on the lung endothelium: calcium channels that regulate barrier function.
abstract: Rises in cytosolic calcium are sufficient to initiate the retraction of endothelial cell borders and to increase macromolecular permeability. Although endothelial cell biologists have recognized the importance of shifts in cytosolic calcium for several decades, only recently have we gained a rudimentary understanding of the membrane calcium channels that change cell shape. Members of the transient receptor potential family (TRP) are chief among the molecular candidates for permeability-coupled calcium channels. Activation of calcium entry through store-operated calcium entry channels, most notably TRPC1 and TRPC4, increases lung endothelial cell permeability, as does activation of calcium entry through the TRPV4 channel. However, TRPC1 and TRPC4 channels appear to influence the lung extraalveolar endothelial barrier most prominently, whereas TRPV4 channels appear to influence the lung capillary endothelial barrier most prominently. Thus, phenotypic heterogeneity in ion channel expression and function exists within the lung endothelium, along the arterial-capillary-venous axis, and is coupled to discrete control of endothelial barrier function.
authors.affiliation: Center for Lung Biology, University of South Alabama, Mobile, Alabama 36688, USA.
authors.forename: Donna L
authors.initials: DL
authors.lastname: Cioffi
authors.forename: Kevin
authors.initials: K
authors.lastname: Lowe
authors.forename: Diego F
authors.initials: DF
authors.lastname: Alvarez
authors.forename: Christina
authors.initials: C
authors.lastname: Barry
authors.forename: Troy
authors.initials: T
authors.lastname: Stevens
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002463:Cell Membrane Permeability; D004727:Endothelium / Q000378:metabolism / Q000502:physiology*; D006333:Heart Failure / Q000378:metabolism / Q000503:physiopathology; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D050051:Transient Receptor Potential Channels / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*
chemical_list: D050051:Transient Receptor Potential Channels; D002118:Calcium
references.citation: Eur J Pharmacol. 2005 Mar 14;510(3):217-22
references.pmid: 15763245
references.citation: Am J Physiol Lung Cell Mol Physiol. 2000 Sep;279(3):L419-22
references.pmid: 10956614
references.citation: Am J Respir Crit Care Med. 2005 Nov 1;172(9):1153-60
references.pmid: 16051904
references.citation: Eur J Biochem. 1984 Oct 15;144(2):375-9
references.pmid: 6541575
references.citation: Biochim Biophys Acta. 1982 Jun 28;688(3):691-701
references.pmid: 6889438
references.citation: J Biol Chem. 2003 Oct 3;278(40):39014-9
references.pmid: 12857742
references.citation: Verh K Acad Geneeskd Belg. 1998;60(3):215-50
references.pmid: 9803881
references.citation: Trends Biochem Sci. 2005 Jul;30(7):378-87
references.pmid: 15951181
references.citation: Proc Natl Acad Sci U S A. 1990 Apr;87(7):2466-70
references.pmid: 2138778
references.citation: Physiol Rev. 2005 Apr;85(2):757-810
references.pmid: 15788710
references.citation: Am J Physiol Lung Cell Mol Physiol. 2003 Jan;284(1):L100-7
references.pmid: 12471013
references.citation: Am J Physiol. 1999 Jan;276(1):L41-50
references.pmid: 9887054
references.citation: Mol Biol Cell. 1996 Mar;7(3):419-34
references.pmid: 8868470
references.citation: Nature. 2003 Jul 24;424(6947):434-8
references.pmid: 12879072
references.citation: Cell Biochem Biophys. 2002;37(1):53-70
references.pmid: 12398417
references.citation: Biochem Soc Trans. 2003 Oct;31(Pt 5):912-5
references.pmid: 14505447
references.citation: Biochim Biophys Acta. 2006 Nov;1763(11):1147-60
references.pmid: 17034882
references.citation: J Cell Biol. 2001 Sep 17;154(6):1225-33
references.pmid: 11564759
references.citation: Semin Thromb Hemost. 1996;22(4):309-15
references.pmid: 8944415
references.citation: Novartis Found Symp. 2004;258:18-30; discussion 30-43, 98-102, 263-6
references.pmid: 15104174
references.citation: Cell Calcium. 2003 May-Jun;33(5-6):323-36
references.pmid: 12765679
references.citation: Microvasc Res. 2004 Mar;67(2):139-51
references.pmid: 15020205
references.citation: Nat Cell Biol. 2001 Feb;3(2):121-7
references.pmid: 11175743
references.citation: Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2696-700
references.pmid: 7708708
references.citation: J Biol Chem. 1987 May 5;262(13):6365-72
references.pmid: 3571263
references.citation: Endothelium. 2003;10(1):5-15
references.pmid: 12699072
references.citation: Novartis Found Symp. 2004;258:140-9; discussion 149-59, 263-6
references.pmid: 15104180
references.citation: Neuron. 1994 Jun;12(6):1257-67
references.pmid: 8011336
references.citation: J Cell Biol. 1995 Nov;131(3):655-67
references.pmid: 7593187
references.citation: Curr Biol. 2005 Jul 12;15(13):R493-5
references.pmid: 16005278
references.citation: Gen Physiol Biophys. 1990 Apr;9(2):89-111
references.pmid: 1694153
references.citation: Cell Calcium. 1994 Oct;16(4):259-68
references.pmid: 7820845
references.citation: Nature. 1992 Jul 23;358(6384):325-7
references.pmid: 1322496
references.citation: Am J Physiol. 1998 Aug;275(2):L203-22
references.pmid: 9700080
references.citation: Pflugers Arch. 1995 Jul;430(3):315-22
references.pmid: 7491254
references.citation: J Invest Dermatol. 1989 Aug;93(2 Suppl):62S-67S
references.pmid: 2546995
references.citation: Proc Natl Acad Sci U S A. 2002 May 28;99(11):7461-6
references.pmid: 12032305
references.citation: Semin Hematol. 1983 Oct;20(4):243-60
references.pmid: 6316555
references.citation: J Cell Sci. 2005 Mar 1;118(Pt 5):917-28
references.pmid: 15713749
references.citation: Nature. 1992 Jan 23;355(6358):353-6
references.pmid: 1309940
references.citation: Am J Physiol. 1994 Sep;267(3 Pt 1):L223-41
references.pmid: 7943249
references.citation: Am J Physiol. 1997 Jul;273(1 Pt 1):L22-30
references.pmid: 9252536
references.citation: Trends Mol Med. 2007 Mar;13(3):103-7
references.pmid: 17267286
references.citation: Science. 1985 Nov 29;230(4729):1040-3
references.pmid: 3933112
references.citation: J Biol Chem. 1986 Mar 5;261(7):3310-5
references.pmid: 3949771
references.citation: Microcirculation. 2006 Dec;13(8):693-708
references.pmid: 17085428
references.citation: J Lab Clin Med. 1992 Oct;120(4):513-9
references.pmid: 1402326
references.citation: Circ Res. 2007 Apr 13;100(7):934-6
references.pmid: 17431193
references.citation: Neuron. 1996 Jun;16(6):1189-96
references.pmid: 8663995
references.citation: Vascul Pharmacol. 2002 Nov;39(4-5):173-85
references.pmid: 12747958
references.citation: Am J Physiol. 1999 Jul;277(1):L119-26
references.pmid: 10409238
references.citation: Pharmacol Rev. 1996 Jun;48(2):213-29
references.pmid: 8804104
references.citation: Circ Res. 2005 Oct 28;97(9):853-63
references.pmid: 16254217
references.citation: J Biol Chem. 2002 Apr 19;277(16):13569-77
references.pmid: 11827975
references.citation: Circ Res. 2002 Jul 12;91(1):70-6
references.pmid: 12114324
references.citation: J Biol Chem. 1979 Sep 10;254(17):8620-7
references.pmid: 468843
references.citation: Circ Res. 2005 Nov 25;97(11):1164-72
references.pmid: 16254212
references.citation: Biochim Biophys Acta. 2004 Dec 6;1742(1-3):9-20
references.pmid: 15590052
references.citation: J Biol Chem. 2002 Dec 13;277(50):48303-10
references.pmid: 12377790
references.citation: Am J Physiol Lung Cell Mol Physiol. 2000 Nov;279(5):L815-24
references.pmid: 11053015
references.citation: Nature. 1979 Aug 30;280(5725):811-4
references.pmid: 471052
references.citation: Pflugers Arch. 1998 Jun;436(1):69-74
references.pmid: 9560448
references.citation: J Cell Biol. 1998 Jan 26;140(2):325-34
references.pmid: 9442108
references.citation: Am J Physiol. 1998 May;274(5):L810-9
references.pmid: 9612297
references.citation: J Cell Physiol. 2005 Nov;205(2):262-9
references.pmid: 15880447
references.citation: Am J Physiol Cell Physiol. 2001 Nov;281(5):C1422-33
references.pmid: 11600404
references.citation: Circ Res. 2006 Oct 27;99(9):988-95
references.pmid: 17008604
references.citation: J Physiol. 1993 Jun;465:359-86
references.pmid: 8229840
references.citation: Circ Res. 2005 Apr 29;96(8):856-63
references.pmid: 15790951
references.citation: J Recept Signal Transduct Res. 2006;26(4):225-40
references.pmid: 16818374
references.citation: Physiol Rev. 2006 Jan;86(1):279-367
references.pmid: 16371600
references.citation: Nature. 2007 Mar 15;446(7133):284-7
references.pmid: 17361175
references.citation: Chest. 1992 Aug;102(2):592-607
references.pmid: 1322813
references.citation: J Surg Res. 2007 Nov;143(1):70-7
references.pmid: 17950075
references.citation: Trends Biochem Sci. 1998 Jan;23(1):10-4
references.pmid: 9478128
references.citation: Am J Physiol. 1998 Sep;275(3):L574-82
references.pmid: 9728053
references.citation: J Cell Biol. 1997 Feb 24;136(4):833-44
references.pmid: 9049249
references.citation: J Bioenerg Biomembr. 1994 Oct;26(5):487-93
references.pmid: 7896764
references.citation: FASEB J. 2001 Aug;15(10):1704-1710
references.pmid: 11481220
references.citation: Neuron. 1992 Apr;8(4):643-51
references.pmid: 1314617
references.citation: Physiol Rev. 2001 Oct;81(4):1415-59
references.pmid: 11581493
references.citation: Annu Rev Biochem. 2007;76:387-417
references.pmid: 17579562
references.citation: J Cell Biol. 1980 May;85(2):361-76
references.pmid: 6892816
references.citation: Cell Calcium. 1986 Feb;7(1):1-12
references.pmid: 2420465
references.citation: Neuron. 1992 Apr;8(4):631-42
references.pmid: 1314616
references.citation: J Cell Biol. 1994 Sep;126(5):1183-94
references.pmid: 8063855
references.citation: Trends Pharmacol Sci. 1998 Apr;19(4):131-5
references.pmid: 9612087
references.citation: J Biol Chem. 2007 Nov 30;282(48):34801-8
references.pmid: 17921144
references.citation: Pflugers Arch. 2006 Dec;453(3):303-11
references.pmid: 16944196
references.citation: Neuron. 1989 Apr;2(4):1313-23
references.pmid: 2516726