Validation of an automated detection platform for use with the roche linear array human papillomavirus genotyping test.
An automated platform (BeeBlot) was evaluated in parallel with the recommended protocol for the hybridization and detection steps of the Roche Linear Array human papillomavirus (HPV) genotyping test using DNA from 143 cervical specimens. Genotyping profiles showed 100% concordance between the methods, suggesting that automation could complement the Roche Linear Array for enhanced speed and reproducibility.
Stevens
Matthew P
MP
Department of Microbiology, The Royal Women's Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia. matthew.stevens@mcri.edu.au
Garland
Suzanne M
SM
Tabrizi
Sepehr N
SN
D001331:Automation; D002584:Cervix Uteri / Q000821:virology; D005260:Female; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification* / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
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