title: Thrombin-activatable fibrinolysis inhibitor polymorphisms and recurrent pregnancy loss.
abstract: OBJECTIVE
  To investigate the possible association between selected thrombin-activatable fibrinolysis inhibitor (TAFI) single nucleotide polymorphisms (SNPs) and recurrent pregnancy loss (RPL).
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  One hundred fifty-eight women (86 cases and 72 controls).
  
  
  METHODS
  Determination of TAFI SNPs -438A/G, +505A/G, +1040T/C, +1542C/G, and +1583A/T by polymerase chain reaction (PCR) reactions and sequencing analysis performed on peripheral blood samples.
  
  
  METHODS
  Analysis of the genotype and allele frequencies of TAFI SNPs -438A/G, +505A/G, +1040T/C, +1542C/G, and +1583A/T in women with and without RPL.
  
  
  RESULTS
  Genotype and allele frequencies of TAFI +505 and +1583 SNPs were significantly different in women with RPL compared with control women. The frequencies of the +505A/A and +505G/G genotypes were 1.2% and 61.6% in women with RPL and 13.9% and 43.1% in control women, respectively. The frequencies of the +1583A/A and +1583T/T genotypes were 1.2% and 61.6% in women with RPL and 13.9% and 45.8% in control women, respectively. The genotype and allele frequencies at TAFI position -438, +1040, and +1542 were not significantly different between RPL and control women.
  
  
  CONCLUSIONS
  The SNPs leading to increased TAFI levels are associated with a reduced risk of RPL. It is possible that TAFI is involved in RPL.
authors.affiliation: Department of Internal Medicine, University Tor Vergata, Rome, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Masini
authors.forename: Carlo
authors.initials: C
authors.lastname: Ticconi
authors.forename: Paolo
authors.initials: P
authors.lastname: Gravina
authors.forename: Monica
authors.initials: M
authors.lastname: Tomassini
authors.forename: Adalgisa
authors.initials: A
authors.lastname: Pietropolli
authors.forename: Vittorio
authors.initials: V
authors.lastname: Forte
authors.forename: Giorgio
authors.initials: G
authors.lastname: Federici
authors.forename: Emilio
authors.initials: E
authors.lastname: Piccione
authors.forename: Sergio
authors.initials: S
authors.lastname: Bernardini
mesh_terms: D000026:Abortion, Habitual / Q000453:epidemiology*; D000328:Adult; D025901:Carboxypeptidase B2 / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D006801:Humans; D015994:Incidence; D007558:Italy / Q000453:epidemiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012008:Recurrence; D018570:Risk Assessment; D012307:Risk Factors; D055815:Young Adult
chemical_list: D025901:Carboxypeptidase B2