Radiolytic ligation of oligodeoxynucleotides possessing disulfide bond.
Hypoxic X-radiolysis of diluted aqueous solutions was performed to generate hydrated electrons that induced one-electron reduction of oligodeoxynucleotides (ODNs) possessing a disulfide bond. Upon hypoxic irradiation, reductive ligation reaction between two types of ODNs occurred in the presence of a template ODN strand, resulting in the formation of a prescribed ODN in substantial amounts.
Tanabe
Kazuhito
K
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan. tanabeka@scl.kyoto-u.ac.jp
Kuraseko
Emi
E
Matsumoto
Eiji
E
Yamada
Hisatsugu
H
Ito
Takeo
T
Nishimoto
Sei-ichi
S
D004220:Disulfides / Q000737:chemistry*; D004583:Electrons; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000528:radiation effects; D010084:Oxidation-Reduction; D014965:X-Rays
D004220:Disulfides; D009838:Oligodeoxyribonucleotides