Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake.
OBJECTIVE
To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland.


METHODS
Isolates presenting MICs of nalidixic acid or ciprofloxacin > or = 1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids were transferred by transformation, and further analysis of the genetic structures surrounding the qnrS2 gene was carried out by PCR and sequencing.


RESULTS
A qnrS2 gene was identified from a single Aeromonas allosaccharophila isolate (Lugano lake, Lugano), as part of a mobile insertion cassette located on a broad host range IncU-type plasmid. This plasmid co-harboured a class 1 integron containing the aac(6')-Ib-cr, bla(OXA-1), catB3 and arr-3 gene cassettes.


CONCLUSIONS
These findings strengthen further the role of Aeromonas spp. as a reservoir of antimicrobial resistance determinants in the environment.
Picão
Renata Cristina
RC
Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France.
Poirel
Laurent
L
Demarta
Antonella
A
Silva
Carla Sofia Ferreira
CS
Corvaglia
Anna Rita
AR
Petrini
Orlando
O
Nordmann
Patrice
P
D000333:Aeromonas / Q000187:drug effects* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005618:Fresh Water / Q000382:microbiology*; D023061:Gene Order; D005798:Genes, Bacterial; D032023:Integrons; D020071:Interspersed Repetitive Sequences; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D013557:Switzerland
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015363:Quinolones