title: Magnetic field affects enzymatic ATP synthesis.
abstract: The rate of ATP synthesis by creatine kinase extracted from V. xanthia venom was shown to depend on the magnetic field. The yield of ATP produced by enzymes with 24Mg2+ and 26Mg2+ ions in catalytic sites increases by 7-8% at 55 mT and then decreases at 80 mT. For enzyme with 25Mg2+ ion in a catalytic site, the ATP yield increases by 50% and 70% in the fields 55 and 80 mT, respectively. In the Earth field the rate of ATP synthesis by enzyme, in which Mg2+ ion has magnetic nucleus 25Mg, is 2.5 times higher than that by enzymes, in which Mg2+ ion has nonmagnetic, spinless nuclei 24Mg or 26Mg. Both magnetic field effect and magnetic isotope effect demonstrate that the ATP synthesis is an ion-radical process, affected by Zeeman interaction and hyperfine coupling in the intermediate ion-radical pair.
authors.affiliation: N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow 119991, Russia. abuchach@chph.ras.ru
authors.forename: Anatoly L
authors.initials: AL
authors.lastname: Buchachenko
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Kuznetsov
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis* / Q000737:chemistry*; D000818:Animals; D002384:Catalysis; D020134:Catalytic Domain; D003402:Creatine Kinase / Q000737:chemistry* / Q000378:metabolism; D007554:Isotopes; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry*; D008280:Magnetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D010766:Phosphorylation; D014757:Viper Venoms / Q000201:enzymology; D017819:Viperidae
chemical_list: D007554:Isotopes; D014757:Viper Venoms; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D008274:Magnesium