Proteomic analysis of proteins associated with tt-DDE induced toxicity in BEAS-2B cells.
Trans, trans-2,4-decadienal (tt-DDE), a specific type of dienaldehyde, is abundant in heated oils or cooking oil fumes. Ingestion of heated oils and exposure to cooking oil fumes has been suggested to have a great health impact in a variety of organs, including the lungs. Previous studies have demonstrated that acute exposures to high doses of tt-DDE have induced oxidative stress, genotoxicity, and cytotoxicity in human lung cells. The objective in utilizing proteomic techniques of this study was to identify protein biomarkers associated with tt-DDE-induced oxidative stress and cytotoxicity in human bronchial epithelial cells BEAS-2B. Experimental results suggested that DJ-1 and cofilin proteins were protein biomarkers for tt-DDE-induced cytotoxicity and oxidative stress in lung cells. DJ-1 was especially an early biomarker for tt-DDE exposure.
Lin
Pin-Pin
PP
Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Taiwan.
Yang
Ming-Hui
MH
Liao
Pao-Chi
PC
Wu
Hsin-Yi
HY
Chang
Louis W
LW
Tsai
Hui-Ti
HT
Tyan
Yu-Chang
YC
D000111:Acetylcysteine / Q000494:pharmacology; D051339:Actin Depolymerizing Factors / Q000032:analysis / Q000378:metabolism*; D000447:Aldehydes / Q000633:toxicity*; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001980:Bronchi / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism*; D015513:Oncogene Proteins / Q000032:analysis / Q000378:metabolism*; D018384:Oxidative Stress; D000071617:Protein Deglycase DJ-1; D040901:Proteomics
D051339:Actin Depolymerizing Factors; D000447:Aldehydes; D015415:Biomarkers; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; C057349:2,4-decadienal; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1; D000111:Acetylcysteine