A novel biosensor for bovine serum albumin based on fluorescent self-assembled sandwich bilayers.
Fluorescent dyes butyl rhodamine B were assembled via a DL-cystenine intermediate onto quartz wafers whose surface had first adsorbed gold nanoparticles. Hence self-assembled sandwich bilayers with nanocomposite structure were constructed which can be used as a biosensor for bovine serum albumin. The biosensor-based self-assembled monolayers (SAMs) are regenerable and have high sensitivity, five orders of magnitude higher than that of bulk solution phase sensing. The effects of existing forms of dyes on the fluorescence spectra of bilayers in the presence of bovine serum albumin were investigated.
Sun
Xiangying
X
College of Materials Science and Engineering, Huaqiao University, Quanzhou 362021, People's Republic of China. liumy@hqu.edu.cn
Liu
Bin
B
He
Fei
F
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D053768:Metal Nanoparticles / Q000737:chemistry; D015394:Molecular Structure; D012235:Rhodamines / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods*
D012235:Rhodamines; D012996:Solutions; D012710:Serum Albumin, Bovine; C029773:rhodamine B