title: Improvement of differentiation and mineralization of pre-osteoblasts on composite nanofibers of poly(lactic acid) and nanosized bovine bone powder.
abstract: The effect of NBM incorporation in PLA nanofibers on their mechanical properties and the differentiation and mineralization of osteoblasts was studied. At 20% NBM, the Young's modulus of the nanofibers was 37.78 +/- 4.23, significantly larger than that of pure PLA nanofibers. MC3T3-E1 pre-osteoblasts attached to both types of nanofibers and developed full osteogenic phenotypes. A profound effect of NBM on the mineralization of MC3T3-E1 pre-osteoblasts was confirmed, suggesting that NBM/PLA composite nanofibers exhibit properties similar to those of the native collagen-rich mineralized bone matrix, and could therefore serve as a temporary substrate for facilitating the differentiation and mineralization of bone-forming cells.
authors.affiliation: Department of Bioengineering, Hanyang University, Seongdong-gu, Seoul, Republic of Korea.
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Ko
authors.forename: Sung In
authors.initials: SI
authors.lastname: Jeong
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Lee
authors.forename: Heungsoo
authors.initials: H
authors.lastname: Shin
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D001842:Bone and Bones / Q000737:chemistry*; D002113:Calcification, Physiologic; D002417:Cattle; D017931:DNA Primers; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D010006:Osteoblasts / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011208:Powders; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering
chemical_list: D017931:DNA Primers; D011091:Polyesters; D011108:Polymers; D011208:Powders; D019344:Lactic Acid; C033616:poly(lactide)