title: The outcome of phagocytic cell division with infectious cargo depends on single phagosome formation.
abstract: Given that macrophages can proliferate and that certain microbes survive inside phagocytic cells, the question arises as to the post-mitotic distribution of microbial cargo. Using macrophage-like cells we evaluated the post-mitotic distribution of intracellular Cryptococcus yeasts and polystyrene beads by comparing experimental data to a stochastic model. For beads, the post-mitotic distribution was that expected from chance alone. However, for yeast cells the post-mitotic distribution was unequal, implying preferential sorting to one daughter cell. This mechanism for unequal distribution was phagosomal fusion, which effectively reduced the intracellular particle number. Hence, post-mitotic intracellular particle distribution is stochastic, unless microbial and/or host factors promote unequal distribution into daughter cells. In our system unequal cargo distribution appeared to benefit the microbe by promoting host cell exocytosis. Post-mitotic infectious cargo distribution is a new parameter to consider in the study of intracellular pathogens since it could potentially define the outcome of phagocytic-microbial interactions.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, United States of America. yluo@aecom.yu.edu
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Mauricio
authors.initials: M
authors.lastname: Alvarez
authors.forename: Lingchuan
authors.initials: L
authors.lastname: Xia
authors.forename: Arturo
authors.initials: A
authors.lastname: Casadevall
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D003455:Cryptococcus neoformans / Q000276:immunology; D005089:Exocytosis; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D008938:Mitosis; D008954:Models, Biological; D008962:Models, Theoretical; D010586:Phagocytes / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D010588:Phagosomes / Q000276:immunology*; D013045:Species Specificity; D013269:Stochastic Processes
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