2'-Methylseleno modified oligoribonucleotides synthesized for X-ray crystallography.
Site-specifically modified 2'-methylseleno RNA represents a valuable derivative for phasing of X-ray crystallographic data. Several successful applications in three-dimensional structure determination of nucleic acids, such as the Diels-Alder ribozyme,(1) have relied on these modifications.(2) Currently, preparation of 2'-methylseleno modified oligoribonucleotides is restricted to the 2'-O-[(triisopropylsilyl)oxy]methyl (TOM)(3,4,5) and 2'-O-tert.-butyldimethylsilyl (TBDMS) RNA synthesis methods. Recently, we have introduced synthetic routes to 2'-methylseleno phosphoramidite building blocks of all four standard nucleosides, adenosine, uridine, guanosine, cytidine, that are tailored for the 2'-O-bis(acetoxyethoxy)methyl (ACE) RNA solid-phase synthesis method.(6).
Moroder
Holger
H
Institute of Organic Chemistry, Center for Molecular Biosciences Innsbruck, Leopold Franzens University Innsbruck, Innrain 52a, Innsbruck, Austria.
Puffer
Barbara
B
Micura
Ronald
R
D018360:Crystallography, X-Ray; D006151:Guanosine / Q000031:analogs & derivatives / Q000737:chemistry; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016566:Organoselenium Compounds / Q000737:chemistry*
C522827:2'-methylseleno guanosine; D009843:Oligoribonucleotides; D016566:Organoselenium Compounds; D006151:Guanosine