title: Complex of dipeptidyl peptidase II with adenosine deaminase.
abstract: Dipeptidyl peptidase II (DPPII) from bovine kidney cortex and lung was purified to the electrophoretically homogeneous state. The molecular and catalytic characteristics of the enzyme were determined. It was revealed that DPPII preparations possess adenosine deaminase (ADA) activity at all purification steps. For the first time, the ADA-binding ability of DPPII has been shown similar to the well-known ADA-binding enzyme, DPPIV. The dissociation constant of the DPPII-ADA complex was estimated using a resonant mirror biosensor (80 nM), fluorescence polarization (60 nM), and differential spectroscopy (36 nM) techniques. The data demonstrate that DPPII can form a complex with ADA, but with one order of magnitude higher dissociation constant than that of DPPIV (7.8 nM).
authors.affiliation: Buniatyan Institute of Biochemistry, Armenian National Academy of Sciences, Yerevan, Armenia.
authors.forename: S G
authors.initials: SG
authors.lastname: Sharoyan
authors.forename: A A
authors.initials: AA
authors.lastname: Antonyan
authors.forename: S S
authors.initials: SS
authors.lastname: Mardanyan
authors.forename: G
authors.initials: G
authors.lastname: Lupidi
authors.forename: M
authors.initials: M
authors.lastname: Cuccioloni
authors.forename: M
authors.initials: M
authors.lastname: Angeletti
authors.forename: G
authors.initials: G
authors.lastname: Cristalli
mesh_terms: D000243:Adenosine Deaminase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007672:Kidney Cortex / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D046912:Multiprotein Complexes / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D046912:Multiprotein Complexes; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C100151:dipeptidyl peptidase II; D018819:Dipeptidyl Peptidase 4; D000243:Adenosine Deaminase