Pdr12p-dependent and -independent fluorescein extrusion from baker's yeast cells.
Fluorescein efflux from S. cerevisiae cells was measured to study the peculiarities of fluorescein transport system, which is important for yeast resistance to certain drugs and weak organic acid preservatives. Glucose-independent and glucose-stimulated fluorescein effluxes were characterized using iodoacetate, cyanide and orthovanadate, inhibitors of glycolysis, electron transport chain, and ATPases, respectively. It is supposed that in glucose-free medium fluorescein extrusion is ATP-dependent and the energy for this efflux is mainly provided by respiration. In glucose-containing medium, glycolysis plays a critical role for extrusion of fluorescein. The results indicate that acetic acid inhibits the fluorescein efflux from yeast cells. The inhibition constant of glucose-stimulated fluorescein efflux is significantly lower in parental strain than in two mutants defective in PDR12 (ABC-transporter Pdr12p) or WAR1 (transcription factor of Pdr12p). It can be suggested that the membrane protein Pdr12 is involved in fluorescein extrusion from the yeast cells, but component(s) other than Pdr12p is (are) also important.
Lushchak
Volodymyr
V
Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine. lushchak@pu.if.ua
Abrat
Oleksandra
O
Miedzobrodzki
Jacek
J
Semchyshyn
Halyna
H
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D019342:Acetic Acid / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D003486:Cyanides / Q000494:pharmacology; D004734:Energy Metabolism; D019793:Fluorescein / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology
D018528:ATP-Binding Cassette Transporters; D003486:Cyanides; D005456:Fluorescent Dyes; D007461:Iodoacetates; C472673:Pdr12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C472674:War1 protein, S cerevisiae; D014638:Vanadates; D005947:Glucose; D019342:Acetic Acid; D019793:Fluorescein