title: Expanding substrate specificity of nucleoside 2'-deoxyribosyltransferase.
abstract: Nucleoside 2'-deoxyribosyltransferase (NDT) is used to synthesize unnatural 2'-deoxyribonucleosides, modified mostly on the heterocyclic base. Here we describe a strategy for improving 2,3-dideoxyribosyl(ddR) transfer activity of NDT by combining mutagenesis and in vivo selection in E. coli.
authors.affiliation: Institut Pasteur, Unité de Chimie Organique, CNRS, URA2128, 28, rue du Dr Roux, 75724 Paris cedex 15, France. akaminsk@pasteur.fr
authors.forename: Pierre Alexandre
authors.initials: PA
authors.lastname: Kaminski
authors.forename: Laurence
authors.initials: L
authors.lastname: Dugué
authors.forename: Sylvie
authors.initials: S
authors.lastname: Pochet
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D052197:Limosilactobacillus fermentum / Q000201:enzymology; D052199:Lactobacillus leichmannii / Q000201:enzymology; D016296:Mutagenesis; D010430:Pentosyltransferases / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D010430:Pentosyltransferases; C021888:nucleoside deoxyribosyltransferase