Human immunodeficiency viruses: propagation, quantification, and storage.
Described in this unit are basic protocols frequently used in the research of human immunodeficiency viruses (HIVs). Provided are methods for propagating and quantifying HIV, as well as recommendations for long-term storage. Background information about these methods is also provided and includes their advantages, disadvantages, and troubleshooting.
Richards
Kathryn H
KH
University of Massachusetts, Medical School, Worcester, Massachusetts, USA.
Clapham
Paul R
PR
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002460:Cell Line; D002478:Cells, Cultured; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology; D015498:HIV-2 / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000821:virology; D013048:Specimen Handling / Q000379:methods*; D014776:Virus Cultivation / Q000379:methods*