title: QTL analysis of pasta quality using a composite microsatellite and SNP map of durum wheat.
abstract: Bright yellow color, firmness and low cooking loss are important factors for the production of good-quality pasta products. However, the genetic factors underlying those traits are still poorly understood. To fill this gap we developed a population of 93 recombinant inbred lines (RIL) from the cross between experimental line UC1113 (intermediate pasta quality) with the cultivar Kofa (excellent pasta quality). A total of 269 markers, including 23 SNP markers, were arranged on 14 linkage groups covering a total length of 2,140 cM. Samples from each RIL from five different environments were used for complete pasta quality testing and the results from each year were used for QTL analyses. The combined effect of different loci, environment and their interactions were analyzed using factorial ANOVAs for each trait. We identified major QTLs for pasta color on chromosomes 1B, 4B, 6A, 7A and 7B. The 4B QTL was linked to a polymorphic deletion in the Lpx-B1.1 lipoxygenase locus, suggesting that it was associated with pigment degradation during pasta processing. The 7B QTL for pasta color was linked to the Phytoene synthase 1 (Psy-B1) locus suggesting difference in pigment biosynthesis. QTLs affecting pasta firmness and cooking loss were detected on chromosomes 5A and 7B, and in both cases they were overlapping with QTL for grain protein content and wet gluten content. These last two parameters were highly correlated with pasta firmness (R > 0.71) and inversely correlated to cooking loss (R < -0.37). The location and effect of other QTLs affecting grain size and weight, gluten strength, mixing properties, and ash content are also discussed.
authors.affiliation: Department of Plant Sciences, One Shields Av., University of California, Davis, CA 95616, USA.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Chao
authors.forename: F
authors.initials: F
authors.lastname: Manthey
authors.forename: O
authors.initials: O
authors.lastname: Chicaiza
authors.forename: J C
authors.initials: JC
authors.lastname: Brevis
authors.forename: V
authors.initials: V
authors.lastname: Echenique
authors.forename: J
authors.initials: J
authors.lastname: Dubcovsky
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics; D000704:Analysis of Variance; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D005819:Genetic Markers; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D005983:Glutens / Q000032:analysis; D008084:Lipoxygenase / Q000235:genetics; D018895:Microsatellite Repeats; D010858:Pigmentation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D014908:Triticum / Q000737:chemistry / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D005983:Glutens; D008084:Lipoxygenase; D019883:Alkyl and Aryl Transferases; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase
references.citation: Theor Appl Genet. 2008 Mar;116(5):635-45
references.pmid: 18193186
references.citation: Theor Appl Genet. 2005 Feb;110(3):550-60
references.pmid: 15655666
references.citation: J Exp Bot. 2006;57(11):2785-94
references.pmid: 16831844
references.citation: Theor Appl Genet. 2005 Aug;111(3):573-82
references.pmid: 15912342
references.citation: Theor Appl Genet. 2008 Jan;116(2):213-21
references.pmid: 17943267
references.citation: Proc Natl Acad Sci U S A. 2003 May 13;100(10):6263-8
references.pmid: 12730378
references.citation: Hereditas. 2001;135(2-3):255-61
references.pmid: 12152344
references.citation: Theor Appl Genet. 2003 Nov;107(7):1243-51
references.pmid: 12923624
references.citation: Genomics. 1987 Oct;1(2):174-81
references.pmid: 3692487
references.citation: J Agric Food Chem. 2002 Nov 6;50(23):6663-8
references.pmid: 12405758
references.citation: Genetics. 2008 Jan;178(1):489-511
references.pmid: 18202390
references.citation: Biotechniques. 2001 Sep;31(3):560, 562, 564-8, passim
references.pmid: 11570500
references.citation: Theor Appl Genet. 2006 May;112(7):1195-204
references.pmid: 16453131
references.citation: Theor Appl Genet. 2004 Feb;108(3):401-13
references.pmid: 14676946
references.citation: Theor Appl Genet. 2007 Feb;114(3):525-37
references.pmid: 17131106
references.citation: Genome Res. 2003 Aug;13(8):1818-27
references.pmid: 12902377
references.citation: Theor Appl Genet. 2008 Jun;117(1):103-15
references.pmid: 18437346
references.citation: Plant Physiol. 2004 Jul;135(3):1776-83
references.pmid: 15247400