Fluorescence fluctuations analysis in nanoapertures: physical concepts and biological applications.
During the past years, nanophotonics has provided new approaches to study the biological processes below the optical diffraction limit. How single molecules diffuse, bind and assemble can be studied now at the nanometric level, not only in solutions but also in complex and crowded environments such as in live cells. In this context fluorescence fluctuations spectroscopy is a unique tool since it has proven to be easy to use in combination with nanostructures, which are able to confine light in nanometric volumes. We review here recent advances in fluorescence fluctuations' analysis below the optical diffraction limit with a special focus on nanoapertures milled in metallic films. We discuss applications in the field of single-molecule detection, DNA sequencing and membrane organization, and underscore some potential perspectives of this new emerging technology.
Lenne
Pierre-François
PF
Institut Fresnel, UMR 6133 CNRS, Aix-Marseille University and Ecole Centrale Marseille, Domaine Universitaire de Saint Jérôme, 13397, Marseille Cedex 20, France. lenne@fresnel.fr
Rigneault
Hervé
H
Marguet
Didier
D
Wenger
Jérôme
J
D000818:Animals; D004798:Enzymes / Q000032:analysis; D004867:Equipment Design; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D015336:Molecular Probe Techniques / Q000295:instrumentation; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D017422:Sequence Analysis, DNA; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
D004798:Enzymes; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D008565:Membrane Proteins
Biophys J. 2005 Jun;88(6):L43-5
15821167
Opt Express. 2006 Jan 23;14(2):956-69
19503416
Nature. 2007 Jan 4;445(7123):39-46
17203054
Biopolymers. 2007 Feb 5;85(2):131-43
17103421
J Cell Biol. 1987 Aug;105(2):755-60
3624308
Nature. 2006 Jul 27;442(7101):387-93
16871206
Annu Rev Biophys Biomol Struct. 2005;34:351-78
15869394
J Biol Phys. 2006 Jan;32(1):SN1-4
19669430
Anal Biochem. 2005 Feb 15;337(2):171-94
15691498
Opt Express. 2005 Sep 5;13(18):7035-44
19498725
Science. 2003 Jan 31;299(5607):682-6
12560545
Phys Rev Lett. 2005 Sep 9;95(11):117401
16197045
Nano Lett. 2007 Jun;7(6):1749-56
17503872
Appl Opt. 2001 Aug 20;40(24):3969-83
18360431
Biophys J. 2007 Feb 1;92(3):913-9
17085499
Nat Methods. 2008 Jun;5(6):539-44
18488034
Nat Chem Biol. 2008 Sep;4(9):538-47
18641634
Biophys J. 2005 Mar;88(3):2145-53
15613638
EMBO J. 2006 Aug 9;25(15):3446-57
16900097
Biophys J. 2005 Dec;89(6):4029-42
16199500
Annu Rev Biophys Biomol Struct. 2007;36:151-69
17477838
Opt Express. 2006 Jan 23;14(2):832-46
19503403
Nature. 1997 Jun 5;387(6633):569-72
9177342
Curr Pharm Biotechnol. 2006 Feb;7(1):51-66
16472133
Nat Methods. 2006 Feb;3(2):83-9
16432516
Nat Methods. 2006 Oct;3(10):793-5
16896339
Opt Express. 2008 Mar 3;16(5):3008-20
18542387
J Opt Soc Am A Opt Image Sci Vis. 2006 Sep;23(9):2342-8
16912764
Opt Express. 2006 Dec 11;14(25):12206-16
19529650
Nat Rev Mol Cell Biol. 2003 May;4(5):414-8
12728275
Opt Lett. 1999 Jul 15;24(14):954-6
18073907
Annu Rev Biophys. 2008;37:265-88
18573082
Biophys J. 2006 May 1;90(9):3288-99
16461393
Science. 2006 Sep 15;313(5793):1642-5
16902090
J Cell Biol. 2002 Jun 10;157(6):1071-81
12058021
Opt Lett. 2008 May 1;33(9):1026-8
18451975
Biophys J. 1997 Apr;72(4):1878-86
9083691
Proc Natl Acad Sci U S A. 2008 Jan 29;105(4):1176-81
18216253
EMBO J. 2006 Jul 26;25(14 ):3245-56
16858413