title: Preparation and characterization of hexyl methacrylate monolithic columns for CEC.
abstract: The preparation of hexyl methacrylate (HMA) monolithic columns for CEC separations has been investigated with two initiation systems: (i) ammonium peroxodisulphate and TEMED to activate the polymerization reaction, and (ii) by thermal initiation with AIBN. For both initiators, the influence of composition of porogenic solvent on morphological and chromatographic properties of monoliths was investigated. Two porogenic solvent systems, aqueous and non-aqueous media, were also studied for monolithic beds polymerized with AIBN. Under optimal conditions, low minimum plate heights (9.6 mum for peroxodisulphate, 8.4 and 10.0 mum for AIBN in aqueous and non-aqueous porogenic solvents, respectively) were obtained. A comparison in terms of chromatographic performance of HMA monoliths with butyl methacrylate columns polymerized with both initiators was also performed. The resulting HMA-based stationary phases also exhibited a good repeatability and column-to-column reproducibility, with RSD values below 5.6% in the studied electrochromatographic parameters. The potential of HMA-based columns was demonstrated by the analysis of complex mixtures of polyaromatic hydrocarbons and anabolic steroids.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Valencia, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Cantó-Mirapeix
authors.forename: José M
authors.initials: JM
authors.lastname: Herrero-Martínez
authors.forename: Carlos
authors.initials: C
authors.lastname: Mongay-Fernández
authors.forename: Ernesto F
authors.initials: EF
authors.lastname: Simó-Alfonso
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D005029:Ethylenediamines / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D015203:Reproducibility of Results
chemical_list: D000180:Acrylic Resins; D005029:Ethylenediamines; D008689:Methacrylates; C005798:N,N,N',N'-tetramethylethylenediamine; C533795:hexyl methacrylate; C016284:butyl methacrylate