title: Metabolism of 5-isopropyl-6-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(2-methyl-1H-pyrrolo[2,3-b]pyridin-5-yl)pyrrolo[2,1-f][1,2,4]triazin-4-amine (BMS-645737): identification of an unusual N-acetylglucosamine conjugate in the cynomolgus monkey.
abstract: 5-Isopropyl-6-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(2-methyl-1H-pyrrolo[2,3-b]pyridin-5-yl)pyrrolo[2,1-f][1,2,4]triazin-4-amine (BMS-645737) is a potent and selective vascular endothelial growth factor receptor-2 antagonist. In this study, liquid chromatography/tandem mass spectrometry and NMR were used to investigate the biotransformation of BMS-645737 in vitro and in the cynomolgus monkey, dog, mouse, and rat. Metabolic pathways for BMS-645737 included multistep processes involving both oxidation and conjugation reactions. For example, the 2-methyl-1H-pyrrolo moiety underwent cytochrome P450-catalyzed hydroxylation followed by oxidation to a carboxylic acid and then conjugation with taurine. Alternatively, the 5-methyl-1,3,4-oxadiazol-2-yl moiety was metabolized by hydroxylation and then conjugation with sulfate. The pyridin-5-yl group underwent direct glucuronidation in hepatocytes (dog, monkey, human) and conjugation with N-acetylglucosamine in the monkey. Conjugation with glutathione and processing along the mercapturic acid pathway was a minor metabolic pathway in vivo, although BMS-645737 did not form conjugates in the presence of glutathione-supplemented liver microsomes. Other minor biotransformation pathways included oxidative dehydrogenation, dihydroxylation, and hydrolytic opening of the oxadiazole ring followed by either deacetylation or hydrolysis of the resulting diacyl hydrazide. Whereas previous studies have shown the formation of N-acetylglucosamine conjugates of alcohols, arylamines, and other small molecules, this report describes the biotransformation of a heterocyclic aromatic amine via direct conjugation with N-acetylglucosamine.
authors.affiliation: Pharmaceutical Candidate Optimization, Bristol-Myers Squibb Company, 5 Research Parkway, Wallingford, CT 06492, USA. benjamin.m.johnson@bms.com
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Johnson
authors.forename: Amrita V
authors.initials: AV
authors.lastname: Kamath
authors.forename: John E
authors.initials: JE
authors.lastname: Leet
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
authors.forename: Rajeev S
authors.initials: RS
authors.lastname: Bhide
authors.forename: Ravindra W
authors.initials: RW
authors.lastname: Tejwani
authors.forename: Yueping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ligang
authors.initials: L
authors.lastname: Qian
authors.forename: Donna D
authors.initials: DD
authors.lastname: Wei
authors.forename: Louis J
authors.initials: LJ
authors.lastname: Lombardo
authors.forename: Yue-Zhong
authors.initials: YZ
authors.lastname: Shu
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D001646:Bile / Q000378:metabolism; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004285:Dogs; D006031:Glycosylation; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D006900:Hydroxylation; D008252:Macaca fascicularis; D009682:Magnetic Resonance Spectroscopy; D053858:Metabolic Networks and Pathways; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000378:metabolism; D015394:Molecular Structure; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D011758:Pyrroles / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014227:Triazines / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000037:antagonists & inhibitors
chemical_list: C529910:5-isopropyl-6-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(2-methyl-1H-pyrrolo(2,3-b)pyridin-5-yl)pyrrolo(2,1-f)(1,2,4)triazin-4-amine; D047428:Protein Kinase Inhibitors; D011758:Pyrroles; D011994:Recombinant Proteins; D014227:Triazines; D003577:Cytochrome P-450 Enzyme System; D040301:Vascular Endothelial Growth Factor Receptor-2; D000117:Acetylglucosamine