title: In vitro anti-bacterial and anti-adherence effects of natural polyphenolic compounds on oral bacteria.
abstract: OBJECTIVE
  To investigate the action of different polyphenolic compounds, extracted from red wine, grape marc and pine bark, on oral bacteria.
  
  
  RESULTS
  The anti-microbial activity of extracts was examined by determining the Minimal Inhibitory Concentration and Minimal Bactericidal Concentration using the macro dilution broth technique. Their effect on the adherence was tested on growing cells of Streptococcus mutans on a glass surface and on a multi-species biofilm grown on saliva-coated hydroxyapatite discs. The effect on glucosyltransferase activity was analysed through the reductions in the overall reaction rate and the quantity of insoluble glucan (ISG) synthesized. Pine bark and grape marc extracts were the most effective inhibitors of the multi-species biofilm formation and of the ISG synthesis.
  
  
  CONCLUSIONS
  The tested components showed an interesting anti-plaque activity in vitro.
  
  
  CONCLUSIONS
  This is, to our knowledge, the first and the most complete report on the properties of wine and pine bark extracts that could be used for oral disease prevention purpose.
authors.affiliation: Laboratoire de Microbiologie, UFR d'Odontologie, Bordeaux Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Furiga
authors.forename: A
authors.initials: A
authors.lastname: Lonvaud-Funel
authors.forename: G
authors.initials: G
authors.lastname: Dorignac
authors.forename: C
authors.initials: C
authors.lastname: Badet
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D003773:Dental Plaque / Q000517:prevention & control; D005898:Glass; D005936:Glucans / Q000032:analysis / Q000037:antagonists & inhibitors; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000187:drug effects; D006882:Hydroxyapatites; D050296:Microbial Viability / Q000187:drug effects; D009055:Mouth / Q000382:microbiology*; D028223:Pinus; D024301:Plant Bark; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development; D027843:Vitis; D014920:Wine
chemical_list: D000900:Anti-Bacterial Agents; D005936:Glucans; D006882:Hydroxyapatites; D010936:Plant Extracts; C475044:hydroxyapatite cement; D005964:Glucosyltransferases