title: Effects of acid pre-treatment on bio-hydrogen production and microbial communities during dark fermentation.
abstract: Optimal conditions for acid pre-treatment were investigated for the enrichment of hydrogen-producing bacteria (HPB) in a mixed culture using three strong acids: HCl, HNO(3), and H2SO4 x HCl was selected as a suitable acid for the enrichment of HPB in the fermentation process. The volume of bio-hydrogen produced when the mixed culture was pre-treated using HCl at pH 2 was 3.2 times higher than that obtained without acid pre-treatment. Changes in the microbial community during acid pre-treatment were monitored using images obtained by the fluorescent in situ hybridization (FISH) method and the Live/Dead cell viability test. The tests clearly indicated that the Clostridium species of cluster I were the predominant strains involved in bio-H(2) fermentation, and could be selectively enriched by HCl pre-treatment.
authors.affiliation: School of Environment and Applied Chemistry, Center for Environmental Studies, Kyunghee University, Yongin, Republic of Korea.
authors.forename: Myoung-Joo
authors.initials: MJ
authors.lastname: Lee
authors.forename: Ji-Hyeon
authors.initials: JH
authors.lastname: Song
authors.forename: Sun-Jin
authors.initials: SJ
authors.lastname: Hwang
mesh_terms: D000143:Acids / Q000737:chemistry*; D001421:Bacteria, Anaerobic / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D050260:Carbohydrate Metabolism / Q000502:physiology*; D002241:Carbohydrates / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D003013:Clostridium / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*
chemical_list: D000143:Acids; D002241:Carbohydrates; D006859:Hydrogen