Molecular phylogeny of pneumocystis based on 5.8S rRNA gene and the internal transcribed spacers of rRNA gene sequences.
To clarify the phylogenetic relationships and species status of Pneumocystis, the 5.8S rRNA gene and the internal transcribed spacers (ITS, 1 and 2) of Pneumocystis rRNA derived from rat, gerbil and human were amplified, cloned and sequenced. The genetic distance matrix of six Pneumocystis species compared with other fungi like Taphrina and Saccharomyces indicated that the Pneumocystis genus contained multiple species including Pneumocystis from gerbil. The phylogenetic tree also showed that Pneumocystis from human and monkey formed one group and four rodent Pneumocystis formed another group. Among the four members, Pneumocystis wakefieldiae was most closely related to Pneumocystis murina and Pneumocystis carinii, and was least related to gerbil Pneumocystis.
Li
ZiHui
Z
Department of Pathogenic Biology, Capital Medical University, Beijing 100069, China.
Feng
XianMin
X
Lu
SiQi
S
Zhang
Fan
F
Wang
FengYun
F
Huang
Song
S
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005849:Gerbillinae; D006801:Humans; D010802:Phylogeny; D011010:Pneumocystis / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
D017931:DNA Primers; D012340:RNA, Ribosomal, 5.8S