Generation of monoclonal antibody against canine neural-cell adhesion molecule.
A monoclonal antibody, K9BYU, was generated using Escherichia coli recombinant extracellular domain of canine neural-cell adhesion molecule (N-CAM) as an antigen. Immunoreactivity of K9BYU to insect cell recombinant canine N-CAM was demonstrated by Western blotting using Sf9 insect cells transfected with the canine N-CAM gene. In Western blotting against canine brain tissue, K9BYU detected three isoforms of N-CAM that correspond to three major isoforms of human and mouse N-CAM (N-CAM-120, -140, and -180). From these results, K9BYU was considered to be a useful tool for research of canine N-CAM.
Uematsu
Yosuke
Y
Canine-Lab. Inc, Kanda-Ogawa-machi, Chiyoda-ku, Tokyo, Japan.
Yamaguchi
Tomohiro
T
Koike
Ayumi
A
Yagihara
Hiroko
H
Hasegawa
Daisuke
D
Matsuki
Naoaki
N
Ono
Kenichiro
K
Washizu
Tsukimi
T
Arai
Toshiro
T
Bonkobara
Makoto
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000276:immunology*; D017931:DNA Primers; D004285:Dogs; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D017931:DNA Primers; D011994:Recombinant Proteins