[Expression of ER, bcl-2, and p53 mRNA in early hybrid embryos of chicken-quail].
Using artificial insemination, 100 female quails were crossed with 10 male chickens. The eggs were collected and hatched in the same incubator. The sex of live hybrid embryos from 66 to 120 hatch hours was determined using multiply PCR of Wpkci. Total 300 male and female embryos at various hatch times were sampled and the relative mRNA abundance of ER, bcl-2, and p53 in the embryos was detected by RT-PCR using beta-actin as the internal standard. The effects of ER, bcl-2, and p53 on the early embryonic development for hybrids between chicken and quail were analyzed. The results showed ER mRNA expression of female hybrids were higher than male hybrids from 66 to 84 hatch hours with a highly significant difference (P<0.01), which indicated that the sex differentiation of hybrids was perhaps happened between 66 to 84 h of embryo stage. The obvious sequential expression of bcl-2 and p53 in the embryonic development indicated that the bcl-2 and p53 genes had an important effect on the development of the hybrid embryos.
Liao
He-Rong
HR
1. College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. liaoherong@tom.com
Li
Yan
Y
Guo
Xiao-Ling
XL
Qiao
Ai-Jun
AJ
Ma
Wen-Xia
WX
Zhao
Zong-Sheng
ZS
Zhao
Xiao-Feng
XF
Li
Da-Quan
DQ
Xu
Ning-Ying
NY
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002678:Chimera / Q000196:embryology / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D011784:Quail / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D016159:Tumor Suppressor Protein p53