Determination of azelnidipine by LC-ESI-MS and its application to a pharmacokinetic study in healthy Chinese volunteers.
A simple, rapid and sensitive high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) assay for determination of azelnidipine in human plasma using perospirone as the internal standard (IS) was established. After adjustment to a basic pH with sodium hydroxide solution, plasma samples were extracted with diethyl ether and separated on a C18 column with a mobile phase of methanol-5 mM ammonium acetate solution (90:10, v/v). The lower limit of quantification (LLOQ) was 0.20 ng/ml. After administration of a single dose of azelnidipine 8mg and 16 mg, respectively; the area under the plasma concentration versus time curve from time 0 h to 96 h (AUC(0-96) were (186 +/- 47) ng ml(-1) h, (429 +/- 145) ng ml(-1) h, respectively; clearance rate (CL/F) were (45.94 +/- 11.61), (42.11 +/- 14.23) L/h, respectively; peak plasma concentration Cmax were (8.66 +/- 1.15), (19.17 +/- 4.13) ng/ml, respectively; apparent volume of distribution (Vd) were (1749 +/- 964), (2480 +/- 2212) L, respectively; time to Cmax (Tmax) were (2.8 +/- 1.2), (3.0 +/- 0.9) h, respectively; elimination half-life (t(1/2beta)) were (22.8 +/- 2.4), (23.5 +/- 4.2) h, respectively; and MRT were (25.7 +/- 1.3), (26.2 +/- 2.2) h, respectively; The essential pharmacokinetic parameters after oral multiple doses (8 mg, q.d.) were as follows: (Cmax) ss, (15.04 +/- 2.27) ng/ml; (Tmax) ss, (2.38 +/- 0.92) h; (Cmin) ss, (3.83 +/- 0.94) ng/ml; C(av), (7.05 +/- 1.54) ng/ml; DF, (1.62 +/- 0.26); AUCss, (169.19 +/- 36.87) ng ml(-1) h.
Zou
Jian-Jun
JJ
Nanjing First Hospital Affiliate To Nanjing Medical University, Nanjing City, China.
Ji
Hong-Jian
HJ
Zhou
Xiao-Hua
XH
Zhu
Yu-Bin
YB
Fan
Hong-Wei
HW
Xiao
Da-Wei
DW
Hu
Qin
Q
D000328:Adult; D014150:Antipsychotic Agents / Q000097:blood; D001383:Azetidinecarboxylic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics; D002121:Calcium Channel Blockers / Q000032:analysis* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004095:Dihydropyridines / Q000032:analysis* / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D054833:Isoindoles / Q000097:blood; D008297:Male; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013844:Thiazoles / Q000097:blood
D014150:Antipsychotic Agents; D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D054833:Isoindoles; D013844:Thiazoles; D001383:Azetidinecarboxylic Acid; C065533:perospirone; C061679:azelnidipine