Direct PCR of intact bacteria (colony PCR).
This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using polymerase chain reaction (PCR). This method is commonly referred to as colony PCR.
Woodman
Michael E
ME
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA.
D001419:Bacteria / Q000235:genetics*; D015169:Colony Count, Microbial / Q000379:methods*; D004586:Electrophoresis; D016133:Polymerase Chain Reaction / Q000379:methods*