title: Serotype-specificity of recombinant fusion proteins containing domain III of dengue virus.
abstract: Here, the antigenic specificity of the recombinant fusion proteins containing aa 286-426 of the dengue envelope protein fused to P64k from Neisseria meningitidis and the cross-reactive antibody response induced in immunized mice and monkeys were evaluated. The anti-dengue mice antibodies showed a higher reactivity to the homologous recombinant proteins compared to the wide cross-reactivity observed by dot blot to the viral antigens. The immune response induced by the recombinant proteins in mice and monkeys, was highly serotype specific. The serotype-specificity associated with these recombinant proteins in addition to the high antigenicity, immunogenicity and protecting capacity suggest their advantages as possible vaccine candidates.
authors.affiliation: Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía, km 6 1/2 P.O. Box: 601 Marianao 13, Havana, Cuba.
authors.forename: Alienys
authors.initials: A
authors.lastname: Izquierdo
authors.forename: Lidice
authors.initials: L
authors.lastname: Bernardo
authors.forename: Jorge
authors.initials: J
authors.lastname: Martin
authors.forename: Emidalys
authors.initials: E
authors.lastname: Santana
authors.forename: Lisset
authors.initials: L
authors.lastname: Hermida
authors.forename: Gerardo
authors.initials: G
authors.lastname: Guillén
authors.forename: María G
authors.initials: MG
authors.lastname: Guzmán
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D003715:Dengue / Q000276:immunology* / Q000821:virology; D053059:Dengue Vaccines / Q000737:chemistry / Q000276:immunology; D003716:Dengue Virus / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D008252:Macaca fascicularis; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis / Q000276:immunology; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology; D014709:Vero Cells
chemical_list: D053059:Dengue Vaccines; D000939:Epitopes; D011993:Recombinant Fusion Proteins