title: Particle inflow gun-mediated transformation of multiple-shoot clumps in rhodes grass (Chloris gayana).
abstract: Rhodes grass (Chloris gayana) is one of the most important warm-season forage grasses. It is cultivated in tropical and subtropical parts of the world and is mostly used for grazing and hay production. We have established a particle-bombardment transformation protocol for rhodes grass using multiple-shoot clumps (MSCs) as the target tissue. A vector pAHC25 containing a herbicide-resistance gene (bar) together with the beta-glucuronidase (GUS) gene was used in transformation experiments. The most efficient recovery of bialaphos-resistant tissue was achieved when the bombarded MSCs were first cultured for 15 d on bialaphos-free medium before being subjected to selection pressure. The resistant tissues regenerated transgenic plants that displayed GUS gene expression. Under optimized conditions, 251 target pieces yielded 46 transgenic plants from 4 independent transgenic lines.
authors.affiliation: Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Gondo
authors.forename: Jun
authors.initials: J
authors.lastname: Matsumoto
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tsuruta
authors.forename: Midori
authors.initials: M
authors.lastname: Yoshida
authors.forename: Akira
authors.initials: A
authors.lastname: Kawakami
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Terami
authors.forename: Masumi
authors.initials: M
authors.lastname: Ebina
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Yamada
authors.forename: Ryo
authors.initials: R
authors.lastname: Akashi
mesh_terms: D019470:Biolistics / Q000295:instrumentation* / Q000379:methods*; D018744:DNA, Plant / Q000378:metabolism; D047108:Embryonic Development; D005966:Glucuronidase / Q000378:metabolism; D009928:Organ Specificity; D018520:Plant Shoots / Q000378:metabolism*; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000196:embryology / Q000378:metabolism*; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
chemical_list: D018744:DNA, Plant; D005966:Glucuronidase