title: [The expression of COX-2 mRNA and protein in human laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the expression of COX-2 in both molecule and protein levels in laryngeal squamous cell carcinoma and the relationship between its intensity and histological grade, clinical stage and lymphoma metastasis.
  
  
  METHODS
  By using emi-quantify RT-PCR and Immunohistochemistry, the expression of the COX-2 mRNA and COX-2 protein in laryngeal squamous cell carcinoma and polyp of vocal cord were examined.
  
  
  RESULTS
  Compared with the polyp of vocal cord tissue, the expression level of COX-2 mRNA and COX-2 protein in human laryngeal squamous cell carcinoma were significantly increased (P < 0.01). The intensity of COX-2 mRNA and protein expression increased as the laryngeal squamous cell carcinoma progresses developed.
  
  
  CONCLUSIONS
  COX-2 is high expressed in human laryngeal squamous cell carcinoma, and it may play an significant role in the growth, invasion and metastasis of human laryngeal squamous cell carcinoma. A combination of emi-quantify RT-PCR and immunohistochemistry is sensitive and specific for laryngeal neoplasms detection.
authors.affiliation: Department of Otorhinolaryngology, Chinese and Western Medicine Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430000, China.
authors.forename: Wangyan
authors.initials: W
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Deng
authors.forename: Qi
authors.initials: Q
authors.lastname: Yao
authors.forename: Chengzhang
authors.initials: C
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human