Assays of natural killer (NK) cell ligation to target cells.
Assays for natural killer cells have long been established in the flow repertoire, but successful application of a flow-based protocol requires attention to many details. This unit provides these details in a comprehensive manner. In particular, the assay is designed for simple bench-top cytometers, rather than for more complex research instruments. Following this protocol, even a novice user should be able to achieve successful completion of an NK assay.
Renò
F
F
University of Urbino, Urbino, Italy.
Papa
S
S
Vitale
M
M
Zamai
L
L
D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004867:Equipment Design; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology; D013997:Time Factors