title: Overexpression of CyclinD1 and underexpression of p16 correlate with lymph node metastases in laryngeal squamous cell carcinoma in Chinese patients.
abstract: BACKGROUND
  CyclinD1 and p16 are involved in the regulation of G1 checkpoint and may play an important role in the tumorgenesis of laryngeal squamous cell carcinoma (LSCC). Previous studies have examined the level of expression of cyclinD1 or p16 in LSCC but no such information is available for their relation and their correlation with lymph node metastasis in Chinese patients.
  
  
  METHODS
  A total of 58 patients underwent surgical resection of laryngeal tumours in the Department of Otolaryngology, Qilu Hospital Shandong University between January 2001 and December 2002. All pathologic specimens were available for immunohistochemical study using antibodies against cyclinD1 and p16.
  
  
  RESULTS
  Compared with normal epithelium, expression of CyclinD1 in the LSCC was significantly higher (62.1% vs. 10.0%, P < 0.05), expression of p16 was significantly lower (48.3% vs. 90.0%, P < 0.05); CyclinD1 expression in LSCC was up-regulated in TNM classification (r (s) = 0.409, P < 0.05) as well as with cervical lymph node metastases (r (s) = 0.294, P < 0.05); p16 expression in LSCC was down-regulated with cervical lymph node metastases (r (s) = -0.275, P < 0.05); negative significant correlation between p16 immunostaining and CyclinD1 was observed in LSCC (r (s) = -0.331, P < 0.05).
  
  
  CONCLUSIONS
  There was a negative correlation between CyclinD1 expression and p16 expression in LSCC. The over-expression of CyclinD1 and the under-expression of p16 may play a significant role in the incidence and development of LSCC and may be important indicators for cervical lymph node metastases in Chinese patients of LSCC.
authors.affiliation: Department of Otorhinolaryngology, Shandong Provincial Qianfoshan Hospital, Clinical Medical College of Shandong University, Jinan, China.
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Fu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Ma
authors.forename: Qi-rong
authors.initials: QR
authors.lastname: Wang
authors.forename: Da-peng
authors.initials: DP
authors.lastname: Lei
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Chun-xi
authors.initials: CX
authors.lastname: Liu
authors.forename: Xin-liang
authors.initials: XL
authors.lastname: Pan
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000556:secondary; D002681:China / Q000453:epidemiology; D019938:Cyclin D1 / Q000378:metabolism*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: C530104:CCND1 protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D019938:Cyclin D1
references.citation: CA Cancer J Clin. 2001 Jan-Feb;51(1):15-36
references.pmid: 11577478
references.citation: Int J Cancer. 2001 Jul 20;95(4):209-15
references.pmid: 11400112
references.citation: Cancer Res. 1994 Sep 1;54(17):4813-7
references.pmid: 8062283
references.citation: Genes Dev. 1999 Jun 15;13(12):1501-12
references.pmid: 10385618
references.citation: Cell. 1995 May 5;81(3):323-30
references.pmid: 7736585
references.citation: Science. 1994 Apr 15;264(5157):436-40
references.pmid: 8153634
references.citation: Arch Otolaryngol Head Neck Surg. 1997 Apr;123(4):412-9
references.pmid: 9109790
references.citation: J Laryngol Otol. 2004 Sep;118(9):676-80
references.pmid: 15509364
references.citation: Clin Cancer Res. 1997 Dec;3(12 Pt 1):2229-36
references.pmid: 9815619
references.citation: World J Surg Oncol. 2006 Sep 05;4:62
references.pmid: 16953893
references.citation: J Laryngol Otol. 1997 Jul;111(7):622-6
references.pmid: 9282199
references.citation: J Natl Cancer Inst. 1997 Aug 6;89(15):1161-3
references.pmid: 9262255
references.citation: Eur J Cancer B Oral Oncol. 1996 Sep;32B(5):293-305
references.pmid: 8944832
references.citation: Hum Pathol. 1999 Sep;30(9):1013-8
references.pmid: 10492034
references.citation: Nature. 1993 Dec 16;366(6456):704-7
references.pmid: 8259215
references.citation: Oncogene. 1995 Feb 16;10(4):775-8
references.pmid: 7862456
references.citation: N Engl J Med. 2001 Dec 27;345(26):1890-900
references.pmid: 11756581
references.citation: Lab Invest. 1999 Mar;79(3):255-60
references.pmid: 10092061
references.citation: J Clin Oncol. 1998 Sep;16(9):3069-77
references.pmid: 9738577
references.citation: Laryngoscope. 2002 Mar;112(3):472-81
references.pmid: 12148857
references.citation: Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6289-93
references.pmid: 7603984
references.citation: Virchows Arch. 2003 Jan;442(1):1-7
references.pmid: 12536308
references.citation: Cancer Res. 1995 Nov 1;55(21):4818-23
references.pmid: 7585513
references.citation: J Histochem Cytochem. 1997 Nov;45(11):1455-9
references.pmid: 9358847