title: Comparative pathogenicity of early and recent isolates of avian metapneumovirus subtype C in turkeys.
abstract: The objective of the present study was to compare the pathogenicity of early and recent isolates of avian metapneumovirus subtype-C (aMPV-C) in turkeys. Two-week-old turkeys were inoculated with early and recent isolates of aMPV-C. Clinical signs were monitored. Tissues were examined for viral ribonucleic acid (RNA), lesions, and viral antigen by reverse transcription-polymerase chain reaction (RT-PCR), histopathology and immunohistochemistry, respectively. Birds infected with the recent isolate had higher clinical sign scores than those infected with the early isolate. Only the recent isolate produced a multifocal loss of cilia in the nasal turbinate of infected birds. Immunohistochemistry revealed intense staining of aMPV antigen in turbinate and trachea of birds infected with the recent isolate. The findings indicate that the recent isolate produced more severe clinical signs and lesions in turkeys compared to the early isolate. The recent isolate could be ideal for the development of a challenge model for aMPV infection in turkeys.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, Minnesota 55108, USA.
authors.forename: Binu T
authors.initials: BT
authors.lastname: Velayudhan
authors.forename: Sally L
authors.initials: SL
authors.lastname: Noll
authors.forename: Anil J
authors.initials: AJ
authors.lastname: Thachil
authors.forename: David A
authors.initials: DA
authors.lastname: Halvorson
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Shaw
authors.forename: Sagar M
authors.initials: SM
authors.lastname: Goyal
authors.forename: Kakambi V
authors.initials: KV
authors.lastname: Nagaraja
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D029121:Metapneumovirus / Q000472:pathogenicity*; D018184:Paramyxoviridae Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology / Q000473:pathology* / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014422:Turkeys
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral
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