Reporters of gene expression: autofluorescent proteins.
This unit on GFP provides clear, detailed, easy-to-follow directions for successful transduction of GFP into appropriate cell lines, as well as carefully detailed strategy and trouble-shooting sections. Instructions for sorting and purification of successfully transduced cells and for analysis and re-analysis of cells together with sample data are all included. The discussion provides data on multiple GFP mutants and lists relevant sources and suggested reading material.
Rasko
J E
JE
Centenary Institute of Cancer Medicine and Cell Biology, Sydney, Australia.
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis*; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis*; D009154:Mutation
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins