Synthesis and biochemical studies of tetraphosphate 5' mRNA cap analogs bearing bisphosphonate modification.
Dinucleotide cap analogs containing tetraphosphate bridge modified by replacing one of the bridging oxygen atoms with methylene group were synthesized. The analogs have been examined for their stability towards enzymatic hydrolysis by human DcpS and binding affinity for eIF4E. Their inhibitory properties in rabbit reticulocyte lysate (RRL) translational system were studied.
Rydzik
Anna
A
Division of Biophysics, Institute of Experimental Physics, University of Warsaw, 02-089 Warsaw, Poland.
Zuberek
Joanna
J
Kowalska
Joanna
J
Lukaszewicz
Maciej
M
Darzynkiewicz
Edward
E
Jemielity
Jacek
J
D000818:Animals; D004164:Diphosphonates / Q000737:chemistry*; D004722:Endoribonucleases / Q000378:metabolism; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D006801:Humans; D014176:Protein Biosynthesis; D012314:RNA Cap Analogs / Q000138:chemical synthesis / Q000737:chemistry*; D011817:Rabbits
D004164:Diphosphonates; D039561:Eukaryotic Initiation Factor-4E; D012314:RNA Cap Analogs; D004722:Endoribonucleases; C485332:DcpS protein, human