Association of cytokine gene polymorphisms with bronchial asthma in Macedonians.
Bronchial asthma is a multifactorial disease whereby both environmental and genetic factors contribute to its aetiology and/or clinical severity. The aim of this study was to examine the association of 22 cytokine gene polymorphism in the Macedonian population with bronchial asthma (BA). The sample of the population comprised of 301 normal unrelated individuals and 74 patients with BA. Cytokine genotyping was performed by PCR. Susceptible cytokine polymorphisms for BA for ten genotypes (IL-4 -1098/T:T, TNF-alpha -238/A:G, IL-4 -590/C:C, IL-2 +166/T:T, IL-2 -330/T:T, IL-10 -1082/G:G, IFNgamma utr5644/T:T, IL-10 -1082/A:A, IL-1beta +3962/T:T, IL-6 -174/G:G), six diplotypes, four haplotypes, and two alleles were found. Protective cytokine polymorphisms for BA for seven cytokine genotypes (IL-4 -1098/G:T, TNF- alpha -238/G:G, IL-2 -330/G:T, IL-4 -590/C:T, IFNgamma utr5644/A:T, IL-1beta +3962/C:T, IL-10 -1082/A:G), six cytokine diplotypes, four cytokine haplotypes, and four cytokine alleles were found. We concluded that several cytokine polymorphisms are protective, or susceptible associated with BA in population of Macedonians.
Trajkov
Dejan
D
Institute of Immunobiology and Human Genetics, Faculty of Medicine, University "St. Kiril and Metodij", Skopje, Republic of Macedonia.
Mirkovska-Stojkovikj
Jagoda
J
Arsov
Todor
T
Petlichkovski
Aleksandar
A
Strezova
Ana
A
Efinska-Mladenovska
Olivija
O
Sandevska
Emilija
E
Gogusev
Jean
J
Spiroski
Mirko
M
D000328:Adult; D001249:Asthma / Q000235:genetics* / Q000276:immunology; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D005260:Female; D005787:Gene Frequency / Q000276:immunology; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D018843:Republic of North Macedonia
D016207:Cytokines; D007376:Interleukin-2