title: Comparison of a solid phase human- versus porcine- thyrotropin receptor-based immunoassay for the measurement of thyrotropin receptor antibodies in patients with thyroid diseases.
abstract: Thyrotropin receptor (TSHR) antibody (TRAb) assays based on human or porcine TSHR which are coated to a solid phase are the most commonly used detection methods in clinical practice for patients with thyroid diseases. Yet the difference of the diagnostic values of the two TRAb assays is largely unclear. The aim of our present study was to evaluate the clinical perfomance of a solid phase porcine TRAb assay based on an ELISA technique (TRAb-porcine) and a human TRAb assay based on a chemiluminescence signal detection procedure (TRAb-human). Of 158 patients enrolled in the study, 84 suffered from Graves' disease (GD), 34 had Hashimoto's thyroiditis (HT) and 40 had euthyroid nodular thyroid disease (NTD) without signs of autoimmunity. TRAb measurements were performed according to the manufacturer's instructions. The mean values of TRAb titers detected by the TRAb-human and TRAb-porcine assays in patients with GD were 12.14+/-10.80 IU/L and 15.27+/-13.65 IU/L, respectively. TRAb were detected in 80 and 78 out of 84 GD patients by the TRAb-human and TRAb-porcine assay, respectively. The diagnostic sensitivity of the TRAb-human and TRAb-porcine immunoassay was 95.2 and 92.9% respectively, by 100% specificity of both methods. TRAb values in GD patients detected by the TRAb-human and TRAb-porcine assays were significantly correlated (r=0.929, p<0.0001). Our results indicate that the second generation TRAb-porcine assay based on solid phase technology had a slightly lower diagnostic sensitivity compared to the TRAb-human assay.
authors.affiliation: Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Duesseldorf, Duesseldorf, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Hermsen
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Domberg
authors.forename: C
authors.initials: C
authors.lastname: Graeber
authors.forename: J
authors.initials: J
authors.lastname: Feldkamp
authors.forename: Y
authors.initials: Y
authors.lastname: Duan
authors.forename: K
authors.initials: K
authors.lastname: Xu
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Mao
authors.forename: W A
authors.initials: WA
authors.lastname: Scherbaum
authors.forename: M
authors.initials: M
authors.lastname: Schott
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D006044:Goiter, Nodular / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D006111:Graves Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D050031:Hashimoto Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D018828:Immunoglobulins, Thyroid-Stimulating / Q000032:analysis* / Q000097:blood; D007453:Iodide Peroxidase / Q000276:immunology; D008163:Luminescent Measurements / Q000379:methods*; D011988:Receptors, Thyroid Hormone / Q000276:immunology; D011989:Receptors, Thyrotropin / Q000276:immunology*; D013552:Swine; D013959:Thyroid Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D018828:Immunoglobulins, Thyroid-Stimulating; D011988:Receptors, Thyroid Hormone; D011989:Receptors, Thyrotropin; D007453:Iodide Peroxidase