[Influence of chronic exposure to low doses of gamma-radiation and 90Sr on the level of DNA breaks and cell sensitivity to hydrogen peroxide in the mouse spleen].
Using comet assay, a statistically significant increase (p < 0.05) in the level of DNA breaks in spleen cells was revealed in male CBA/lac mice exposed to gamma-radiation (1.7 cGy/day) or 90Sr (150-250 Bq/day) for 210 days. The level of DNA breaks also increased under combined exposure to both gamma-radiation and 90Sr (p < 0.05), but to a lesser degree than under exposure to each of these factors alone. Upon additional in vitro treatment of spleen cells with hydrogen peroxide, the relative increase in the level of DNA breaks was smaller in cells of irradiated mice than in the control. The ratio of the level of DNA breaks after hydrogen peroxide treatment to that before this treatment in control mice was 4.2 +/- 0.9, compared to 1.4 +/- 0.6 in gamma-irradiated mice, 1.9 +/- 0.8 in 90Sr-irradiated mice, and 2.3 +/- 0.8 in mice exposed to both gamma- and 90Sr-irradiation.
Lizunova
E Iu
EIu
Vorob'eva
N Iu
NIu
Osipov
A N
AN
D000818:Animals; D020552:Comet Assay / Q000379:methods; D053960:DNA Breaks / Q000187:drug effects* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology*; D013326:Strontium Radioisotopes / Q000737:chemistry*
D013326:Strontium Radioisotopes; D006861:Hydrogen Peroxide