RNA containing pyrrolocytidine base analogs: increased binding affinity and fluorescence that responds to hybridization.
6-Phenylpyrrolocytidine and 6-methoxymethylene-pyrrolocytidine are base-modified nucleosides with remarkable fluorescence properties. These analogs produce increased binding affinity to both RNA and DNA targets when incorporated into oligoribonucleotides. The fluorescence observed for the single-stranded oligomers is quenched upon duplex formation with either RNA or DNA targets. The fluorescence response depends on the nature of the 6-substituent and the sequence position of the modified nucleoside.
Wahba
Alexander S
AS
Department of Chemistry, McGill University, Montreal, QC, Canada.
Damha
Masad J
MJ
Hudson
Robert H E
RH
D003562:Cytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D015347:RNA Probes / Q000737:chemistry*
C541629:6-methoxymethylenepyrrolocytidine; C541628:6-phenylpyrrolocytidine; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011758:Pyrroles; D015347:RNA Probes; D003562:Cytidine; D012313:RNA; D004247:DNA