[Expression characteristics of prolactin gene in Eastern Zhejiang white geese].
This study was conducted to clone the prolactin gene (PRL) in Eastern Zhejiang White Geese and to investigate the PRL gene expression characteristics during egg-laying, out-of-lay and incubating periods by real time PCR. Comparisons were made respectively of concentration of prolactin mRNA in the hypothalamus, pituitary gland and ovary of the adult female geese at different reproductive periods. The result indicated that there were significant differences (P<0.05) in PRL mRNA expression between different reproductive periods of the geese. The lowest level of PRL expression was found in out-of-lay geese, higher in the egg-laying geese, and the highest in incubating geese. Furthermore, the analysis of PRL expression in different tissues indicated that the highest levels of PRL was expressed in the pituitary gland, followed in hypothalamus, and the least in ovary of the geese. There were significant difference (P<0.01) expression of PRL between the pituitary gland/hypothalamus and ovary of the geese, whereas no any difference was observed between the pituitary gland and hypothalamus (P>0.05). In summary, the PRL mRNA expression had variance in different reproductive periods of the geese.
Chu
Xiao-Hong
XH
College of Animal Science, Zhejiang University, Hangzhou 310029, China. chuxh@yahoo.cn
Xu
Ning-Ying
NY
Hu
Jin-Ping
JP
Lu
Li-Zhi
LZ
Chen
Wei-Hu
WH
Wang
Ya-Qin
YQ
D000818:Animals; D005260:Female; D005777:Geese; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D007031:Hypothalamus / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011388:Prolactin / Q000235:genetics*
D011388:Prolactin