title: Physiological and morphological characterization of tert -butylhydroperoxide tolerant Candida albicans mutants.
abstract: tert -Butylhydroperoxide (t BOOH) tolerant Candida albicans mutants developed from clinical isolates were characterized with increased tolerance of the oxidative stress generating agents t BOOH and H2O2, continuous induction of the antioxidative defence system, reduced pseudohypha and hypha-forming capabilities, decreased phospholipase secretion and delayed growth in Sabouraud dextrose agar and broth media. Changes in antimycotic (fluconazole, voriconazole, amphotericin B, 5-fluorocytosine) tolerances as well as in total and cytochrome c-dependent respirations showed versatile patterns, meanwhile the intensified alternative oxidase-dependent respiration of the mutants indicated that this respiratory pathway was an important element of the antioxidative defence in general. Because the phenotypes of increased oxidative stress tolerance and reduced virulence attribute production always emerged concomitantly in t BOOH-tolerant mutants the natural selection of C. albicans strains more tolerant of oxidative stress is unlikely. Not surprisingly, a screening study failed to detect any C. albicans strains with increased oxidative stress tolerance among 46 randomly selected clinical isolates.
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fekete
authors.forename: Imre
authors.initials: I
authors.lastname: Pócsi
authors.forename: Tamás
authors.initials: T
authors.lastname: Emri
authors.forename: Agnes
authors.initials: A
authors.lastname: Gyetvai
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Gazdag
authors.forename: Miklós
authors.initials: M
authors.lastname: Pesti
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Karányi
authors.forename: László
authors.initials: L
authors.lastname: Majoros
authors.forename: Lajos
authors.initials: L
authors.lastname: Gergely
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
mesh_terms: D002176:Candida albicans / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000378:metabolism; D025301:Hyphae; D009154:Mutation; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D020122:tert-Butylhydroperoxide / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D004271:DNA, Fungal; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide