Accumulation of yolk in a caecilian (Gegeneophis ramaswamii) oocyte: a light and transmission electron microscopic study.
There is a paucity of information on the female reproductive biology of the caecilian amphibians when compared with the other vertebrate groups. Hence, the accumulation of nutrient reserves in the form of yolk and formation of yolk platelets were studied in Gegeneophis ramaswamii, adopting light microscopic histological and transmission electron microscopy analysis. Previtellogenic as well as vitellogenic follicles were observed in appropriate preparations. On the basis of the source and the routes of entry, we identified four types of yolk precursor materials, precursors 1 to 4. The earliest material appearing in the oocyte consists of abundant lipid vesicles during the previtellogenic phase, i.e., much before the follicular epithelium is fully established. This is a contribution from the oocyte mitochondria, which we identified as yolk precursor material 1, and it is autosynthetic. Once the follicle cell-oocyte interface is fully established, there is an accumulation of the principal component of the heterosynthetic yolk by sequestration from the blood through the intercellular spaces between follicle cells in a pinocytic process. This we identified as yolk precursor material 2. There was also an indication of a lipidic yolk material synthesis in the follicle cells sequestered from maternal blood through the follicle cells in an endocytic process in which the macrovilli of follicle cells and the microvilli of the oocyte play a role. This we identified as yolk precursor material 3. Contribution to the yolk of peptidic, glycosidic, and/or lipidic material synthesized in the vitellogenic oocyte was also indicated. This we identified as yolk precursor material 4. The sequential development of intercellular associations and indications of synthesis/sequestration of the yolk have been traced. Thus, we report the mechanistic details of synthesis/sequestration of the yolk materials in a caecilian.
Beyo
Reston S
RS
Department of Zoology, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala 695581, India.
Divya
Lekha
L
Oommen
Oommen V
OV
Akbarsha
Mohammad A
MA
D000663:Amphibians / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D022162:Cytoplasmic Vesicles / Q000648:ultrastructure; D004530:Egg Yolk / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D018870:Endoplasmic Reticulum, Rough / Q000648:ultrastructure; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D007365:Intercellular Junctions / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000648:ultrastructure; D008954:Models, Biological; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D009866:Oogenesis / Q000502:physiology; D006080:Ovarian Follicle / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D014818:Vitellogenesis / Q000502:physiology*