Characterization of rotavirus specific B cells and their relation with serological memory.
We quantified circulating total, rotavirus (RV) and Tetanus toxin (TT) memory B cells (mBc) in healthy adults using a limiting dilution assay (LDA) and a flow cytometry assay (FCA) that permit evaluation of both CD27+ and CD27- mBc. RV mBc were enriched in the CD27-, IgG+ and in the CD27+, IgM+ subsets. The numbers of RV mBc were higher by FCA than by LDA and results of the two assays did not correlate. TT IgGmBc and RV IgA mBc determined by FCA and by LDA correlated with TT plasma IgG and RV plasma IgA, respectively. The mean ratio of specific mBc/mug/ml of the corresponding plasma immunoglobulin was lower for TT IgG than for RV IgA mBc. Our studies contribute to understand the relationship between circulating mBc and serological memory, and enhance our capacity to develop better correlates of protection against RV disease.
Rojas
Olga Lucía
OL
Instituto de Genética Humana, Pontificia Universidad Javeriana, Carrera 7 # 40-62, Bogotá, Colombia.
Narváez
Carlos Fernando
CF
Greenberg
Harry B
HB
Angel
Juana
J
Franco
Manuel A
MA
D000328:Adult; D000914:Antibodies, Viral / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000097:blood; D007156:Immunologic Memory; D016131:Lymphocyte Subsets / Q000276:immunology*; D012401:Rotavirus / Q000276:immunology*; D013743:Tetanus Antitoxin / Q000097:blood; D013744:Tetanus Toxin / Q000276:immunology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000032:analysis
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013743:Tetanus Antitoxin; D013744:Tetanus Toxin; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
Eur J Immunol. 2005 Oct;35(10):2789-92
16180256
J Immunol. 2004 Oct 1;173(7):4479-91
15383579
J Exp Med. 1990 Jan 1;171(1):19-34
2153188
J Biol Chem. 2001 Aug 3;276(31):29361-7
11356839
Immunity. 2007 Feb;26(2):205-13
17306569
Clin Exp Immunol. 2005 Aug;141(2):279-87
15996192
J Virol. 2004 Oct;78(20):10967-76
15452217
Vaccine. 2006 Apr 5;24(15):2718-31
16446014
J Immunol Methods. 2004 Mar;286(1-2):111-22
15087226
Eur J Immunol. 2001 Jan;31(1):243-9
11265640
Eur J Immunol. 2005 Dec;35(12):3433-41
16259010
J Immunol. 2005 Mar 15;174(6):3454-60
15749880
Curr Opin Gastroenterol. 2007 Jan;23(1):39-43
17133083
J Virol. 2004 Nov;78(22):12489-96
15507636
J Exp Med. 1998 Nov 2;188(9):1679-89
9802980
Eur J Immunol. 1983 Sep;13(9):733-8
6604634
Clin Exp Immunol. 1994 May;96(2):356-63
8187345
Eur J Immunol. 1999 Apr;29(4):1406-17
10229109
Viral Immunol. 2007 Summer;20(2):300-11
17603846
Nat Rev Microbiol. 2007 Jul;5(7):529-39
17571094
J Immunol. 2008 Mar 15;180(6):3980-9
18322207
Mol Immunol. 2007 Mar;44(9):2173-83
17196657
Immunol Rev. 2006 Jun;211:303-9
16824137
Virology. 2003 Jan 5;305(1):93-105
12504544
J Exp Med. 2008 Jun 9;205(6):1331-42
18519648
Science. 2002 Dec 13;298(5601):2199-202
12481138
J Virol. 2007 Jan;81(1):215-28
17050593
Immunol Rev. 2006 Jun;211:295-302
16824136
N Engl J Med. 2007 Nov 8;357(19):1903-15
17989383
J Immunol. 2002 Mar 1;168(5):2173-81
11859103
Vaccine. 2001 Nov 12;20(3-4):498-504
11672915
Vaccine. 2007 Jul 26;25(30):5467-84
17227687
Nat Rev Immunol. 2006 Mar;6(3):231-43
16498452
J Immunol. 2008 Mar 1;180(5):3279-88
18292552
J Immunol. 2006 May 15;176(10):5749-59
16670280
Emerg Infect Dis. 2006 Feb;12(2):304-6
16494759
PLoS Med. 2007 Apr;4(4):e121
17439294
Eur J Immunol. 1983 Oct;13(10):820-4
6227488
J Immunol. 2006 Sep 15;177(6):3728-36
16951333
J Immunol. 2007 May 15;178(10):6624-33
17475894
J Immunol Methods. 2006 Dec 20;317(1-2):175-85
17055526