title: Expression and alternative splicing of N-RAP during mouse skeletal muscle development.
abstract: N-RAP alternative splicing and protein localization were studied in developing skeletal muscle tissue from pre- and postnatal mice and in fusing primary myotubes in culture. Messages encoding N-RAP-s and N-RAP-c, the predominant isoforms of N-RAP detected in adult skeletal muscle and heart, respectively, were present in a 5:1 ratio in skeletal muscle isolated from E16.5 embryos. N-RAP-s mRNA levels increased three-fold over the first 3 weeks of postnatal development, while N-RAP-c mRNA levels remained low. N-RAP alternative splicing during myotube differentiation in culture was similar to the pattern observed in embryonic and neonatal muscle, with N-RAP-s expression increasing and N-RAP-c mRNA levels remaining low. In both developing skeletal muscle and cultured myotubes, N-RAP protein was primarily associated with developing myofibrillar structures containing alpha-actinin, but was not present in mature myofibrils. The results establish that N-RAP-s is the predominant spliced form of N-RAP present throughout skeletal muscle development.
authors.affiliation: Laboratory of Muscle Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892-8024, USA.
authors.forename: Shajia
authors.initials: S
authors.lastname: Lu
authors.forename: Diane E
authors.initials: DE
authors.lastname: Borst
authors.forename: Robert
authors.initials: R
authors.lastname: Horowits
mesh_terms: D000185:Actinin / Q000378:metabolism; D017398:Alternative Splicing; D000818:Animals; D002478:Cells, Cultured; D005091:Exons / Q000235:genetics; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D024510:Muscle Development / Q000235:genetics*; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D009210:Myofibrils / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; C491567:Nrap protein, mouse; D020033:Protein Isoforms; D000185:Actinin
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