[Temporospatial expression of cyclin G1 in normal human endometria].
OBJECTIVE
To study the temporospatial expression of cyclin G1 protein and mRNA in normal human endometrium and its physiological implications.


METHODS
Human endometria samples were taken from 15 women in proliferative phase, 11 in early secretory phase, and 15 in mid-late secretary phase. Cyclin G1 protein and mRNA in the normal human endometria were measured by immunohistochemistry and in situ hybridization, respectively.


RESULTS
The expression of cyclin G1 protein in the epithelium cells was low in proliferative phase. The expression increased at early secretary phase and reached the highest at mid-late secretary phase. The expression of cyclin G1 protein in stromal cells only appeared at mid-late secretary phase. The cyclin G1 protein was localized in nucleus. The expression of cyclin G1 mRNA was localized in cytoplasma, but had similar cycles as the expression of cyclin G1 protein.


CONCLUSIONS
The expression of Cyclin G1 in normal human endometria is progesterone dependence.
Baima
Kang-zhuo
KZ
Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Tian
Ji-Mei
JM
Yang
Kai-xuan
KX
Qie
Ming-rong
MR
Zhang
Jin-hu
JH
He
Ya-ping
YP
Yue
Li-min
LM
D000328:Adult; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins / Q000096:biosynthesis* / Q000235:genetics; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008597:Menstrual Cycle / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013997:Time Factors
C534702:CCNG1 protein, human; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins; D012333:RNA, Messenger