title: Cultivation, LD(50) determination and experimental model of Streptococcus suis serotype 2 strain HA9801.
abstract: The effects of nutritional components and submerged culture conditions on colony-forming unit (CFU) counts by Streptococcus suis serotype 2 strain HA9801 in flask culture was investigated, and the optimal medium and cultivation conditions was confirmed by using a 50l bioreactor. The LD(50) values of HA9801 in pigs before and after fermentation were 1.8 x 10(7)CFU, which indicated that the virulence of HA9801 was very stable in the fermentation process. In addition, an experimental model that closely mimics naturally occurring disease in conventional pigs was established.
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200232, China.
authors.forename: Zhanzhong
authors.initials: Z
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Peihong
authors.initials: P
authors.lastname: Liu
authors.forename: Suhua
authors.initials: S
authors.lastname: Zhang
authors.forename: Jianpei
authors.initials: J
authors.lastname: Gong
authors.forename: Xiqin
authors.initials: X
authors.lastname: Huang
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Feiqun
authors.initials: F
authors.lastname: Xue
mesh_terms: D000818:Animals; D019149:Bioreactors; D015169:Colony Count, Microbial / Q000662:veterinary; D007928:Lethal Dose 50; D008297:Male; D011897:Random Allocation; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D017011:Streptococcus suis / Q000254:growth & development* / Q000472:pathogenicity*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014774:Virulence