Optimized fluoride ions treatment for release of base-sensitive 2'-O-modified oligoribonucleotides from various solid supports.
To synthesize oligoribonucleotides containing base-sensitive 2'-O-modifications, we used the Q-linker or a silyl linker anchored to CPG or polystyrene resin. A fluoride ions treatment was optimized to release oligoribonucleotides from the solid-support and to remove TBDMS groups without affecting 2'-O-acyloxymethyl groups.
Lavergne
Thomas
T
IBMM, UMR 5247 CNRS-UM1-UM2, CC 1704, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
Debart
Françoise
F
Vasseur
Jean-Jacques
JJ
D000577:Amides / Q000737:chemistry; D005459:Fluorides / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
C522070:5-taurinomethyluridine; D000577:Amides; D007477:Ions; D009843:Oligoribonucleotides; D010756:Phosphoric Acids; C011067:phosphoramidic acid; D005459:Fluorides; D014529:Uridine