title: Optimization of culture conditions for hydrogen production by Ethanoligenens harbinense B49 using response surface methodology.
abstract: The design of an optimum and cost-efficient medium for high-level production of hydrogen by Ethanoligenens harbinense B49 was attempted by using response surface methodology (RSM). Based on the Plackett-Burman design, Fe(2+) and Mg(2+) were selected as the most critical nutrient salts. Subsequently, the optimum combination of the selected factors and the sole carbon source glucose were investigated by the Box-Behnken design. Results showed that the maximum hydrogen yield of 2.21 mol/mol glucose was predicted when the concentrations of glucose, Fe(2+) and Mg(2+) were 14.57 g/L, 177.28 mg/L and 691.98 mg/L, respectively. The results were further verified by triplicate experiments. The batch reactors were operated under an optimized condition of the respective glucose, Fe(2+) and Mg(2+) concentration of 14.5 g/L, 180 mg/L and 690 mg/L, the initial pH of 6.0 and experimental temperature of 35+/-1(o)C. Without further pH adjustment, the maximum hydrogen yield of 2.20 mol/mol glucose was obtained based on the optimized medium with further verified the practicability of this optimum strategy.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, PR China.
authors.forename: Wan-Qian
authors.initials: WQ
authors.lastname: Guo
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
authors.forename: Xiang-Jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Xiang
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Yang
authors.initials: Y
authors.lastname: You
authors.forename: Bing-Feng
authors.initials: BF
authors.lastname: Liu
mesh_terms: D000465:Algorithms; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D003013:Clostridium / Q000378:metabolism*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D003198:Computer Simulation; D006859:Hydrogen / Q000378:metabolism*; D008954:Models, Biological; D011786:Quality Control
chemical_list: D006859:Hydrogen