title: Impairment of germline transmission after blastocyst injection with murine embryonic stem cells cultured with mouse hepatitis virus and mouse minute virus.
abstract: The aim of this study was to determine the susceptibility of murine embryonic stem (mESCs) to mouse hepatitis virus (MHV-A59) and mouse minute virus (MMVp) and the effect of these viruses on germline transmission (GLT) and the serological status of recipients and pups. When recipients received 10 blastocysts, each injected with 10(0) TCID(50) MHV-A59, three out of five recipients and four out of 14 pups from three litters became seropositive. When blastocysts were injected with 10(-5) TCID(50) MMVp, all four recipients and 14 pups from four litters remained seronegative. The mESCs replicated MHV-A59 but not MMVp, MHV-A59 being cytolytic for mESCs. Exposure of mESCs to the viruses over four to five passages but not for 6 h affected GLT. Recipients were seropositive for MHV-A59 but not for MMVp when mESCs were cultured with the virus over four or five passages. The data show that GLT is affected by virus-contaminated mESCs.
authors.affiliation: Department of Comparative Medicine, Helmholtz Center Munich, Germany. mahabir@helmholtz-muenchen.de
authors.forename: E
authors.initials: E
authors.lastname: Mahabir
authors.forename: K
authors.initials: K
authors.lastname: Reindl
authors.forename: J
authors.initials: J
authors.lastname: Mysliwietz
authors.forename: J
authors.initials: J
authors.lastname: Needham
authors.forename: D
authors.initials: D
authors.lastname: Bulian
authors.forename: K
authors.initials: K
authors.lastname: Markoullis
authors.forename: H
authors.initials: H
authors.lastname: Scherb
authors.forename: J
authors.initials: J
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000821:virology*; D018352:Coronavirus Infections / Q000821:virology*; D004624:Embryo Transfer; D053595:Embryonic Stem Cells / Q000821:virology*; D005260:Female; D005307:Fertilization in Vitro; D005434:Flow Cytometry; D005854:Germ Cells / Q000502:physiology*; D006524:Hepatitis, Viral, Animal / Q000276:immunology / Q000635:transmission*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008915:Minute Virus of Mice / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*; D006517:Murine hepatitis virus / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*; D010322:Parvoviridae Infections / Q000821:virology*; D011991:Receptors, Virus / Q000378:metabolism; D016036:Seroepidemiologic Studies; D014779:Virus Replication
chemical_list: D011991:Receptors, Virus
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