title: [Effects of Pollen Typhae total flavones on mRNA expressions of peroxisome proliferator-activated receptors in 3T3-L1 adipocytes].
abstract: OBJECTIVE
  To explore the effects of Pollen Typhae total flavones (PTF) on the mRNA expressions of peroxisome proliferator-activated receptors (PPARs) alpha,gamma, beta/delta so as to analyze its possible mechanism in improving the insulin sensitivity of 3T3-L1 adipocytes.
  
  
  METHODS
  Adipocytes were treated with PTF, and expressions of PPARalpha, PPARgamma, PPARbeta/delta mRNAs relating to the adipocyte glucose and lipid metabolism were determined by reverse transcription polymerase chain reaction.
  
  
  RESULTS
  PTF obviously up-regulated the expressions of PPARalpha and PPARgamma mRNAs, all showing significant differences as compared with those in the normal control group (P<0.01).
  
  
  CONCLUSIONS
  With its function as an insulin sensitizer, PTF may enhance the PPARalpha and PPARgamma mRNA expressions in 3T3-L1 adipocytes.
authors.affiliation: Institute of Chinese Integrative Medicine, Fudan University, Shanghai 200040, China.
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: He
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Wang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Ying
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D011058:Pollen / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D032401:Typhaceae / Q000737:chemistry*
chemical_list: D047309:Flavones; D047493:PPAR alpha; D047495:PPAR gamma; D012333:RNA, Messenger