title: Development of a novel capillary electrophoresis chemiluminescence system for amino acid analysis.
abstract: In the present study, a novel peroxyoxalate CE-CL system was developed to achieve high signal stability and sensitivity based on a design of a new interface including a new mixing mode and a new grounding electrode mode. Amino acids fluorescently tagged with dansyl chloride and naphthalene-2,3-dicarboxaldehyde(NDA) were used for the study. Experiment results show this new system is quite effective to separate and detect amine acid with high stability and resolute. The detection limits were 1.1 nmol/L for dansyl-leucine (Leu) and 2.0 nmol/L for dansyl-aspartic acid (Asp). The relative standard deviations of peak height and migration time were in the ranges of 2.3-3.8% and 1.2-1.5%, respectively.
authors.affiliation: College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, People's Republic of China. dongguang_yin@yahoo.com
authors.forename: Dongguang
authors.initials: D
authors.lastname: Yin
authors.forename: Chunjuan
authors.initials: C
authors.lastname: Xie
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Binghu
authors.initials: B
authors.lastname: Liu
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Zhou
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Minghong
authors.initials: M
authors.lastname: Wu
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D001224:Aspartic Acid / Q000032:analysis; D003619:Dansyl Compounds / Q000737:chemistry*; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D007930:Leucine / Q000032:analysis; D008163:Luminescent Measurements / Q000379:methods*; D009281:Naphthalenes / Q000737:chemistry*; D010070:Oxalates / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000596:Amino Acids; D003619:Dansyl Compounds; D009281:Naphthalenes; D010070:Oxalates; D001224:Aspartic Acid; C044622:peroxyoxalate; C027717:2,3-naphthalenedicarboxaldehyde; D007930:Leucine; C029038:dansyl chloride