title: Processing and staining of cell and tissue material for interphase cytogenetics.
abstract: This unit describes in detail the protocols for preparation of biological specimens (fresh or fixed) for the purposes of establishing in situ hybridization procedures using probes for specific regions of the genome, allowing targeted detection of numerical and structural chromosome aberrations in the interphase nucleus. The authors present a series of comprehensive protocols from the simplest to the most complex.
authors.affiliation: University of Maastricht, Maastricht, The Netherlands.
authors.forename: A H
authors.initials: AH
authors.lastname: Hopman
authors.forename: F C
authors.initials: FC
authors.lastname: Ramaekers
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D003582:Cytogenetics; D003584:Cytological Techniques; D016678:Genome; D006801:Humans; D017403:In Situ Hybridization; D007399:Interphase; D015345:Oligonucleotide Probes / Q000032:analysis
chemical_list: D015345:Oligonucleotide Probes