title: Antibodies play a greater role than immune cells in heterologous protection against secondary dengue virus infection in a mouse model.
abstract: The four serotypes of dengue virus (DENV1-4) are causative agents of dengue fever and dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). Previous DENV infection is a risk factor for DHF/DSS during subsequent infection by a different serotype. Nonetheless, most primary and secondary DENV infections are asymptomatic. To investigate the possible mechanisms of immune protection in vivo, 129/Pas mice lacking IFN-alpha/beta and -gamma receptors (AG129) were used to model secondary infection using both DENV1-DENV2 and DENV2-DENV4 sequences. At intervals between sequential infections of 4 to 52 weeks, protection against secondary heterologous DENV infection was observed. Passive transfer of DENV-immune serum was protective against replication of heterologous challenge virus in all tissues tested, whereas adoptive transfer of DENV-immune cells significantly protected mice from replication of the challenge virus only when a lower inoculum was administered. These findings are relevant for understanding both natural and vaccine-induced immunity to DENV.
authors.affiliation: Division of Infectious Diseases, School of Public Health, University of California, Berkeley, 1 Barker Hall #424, Berkeley, CA 94720-7354, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Kyle
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Balsitis
authors.forename: Luhua
authors.initials: L
authors.lastname: Zhang
authors.forename: P Robert
authors.initials: PR
authors.lastname: Beatty
authors.forename: Eva
authors.initials: E
authors.lastname: Harris
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D007116:Immunization, Passive; D051379:Mice; D018345:Mice, Knockout; D017471:Receptors, Interferon / Q000172:deficiency; D019595:Severe Dengue / Q000276:immunology* / Q000517:prevention & control*; D010948:Viral Plaque Assay
chemical_list: D000914:Antibodies, Viral; D017471:Receptors, Interferon
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