Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP.
The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.
Biteen
Julie S
JS
Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, USA.
Thompson
Michael A
MA
Tselentis
Nicole K
NK
Bowman
Grant R
GR
Shapiro
Lucy
L
Moerner
W E
WE
D016935:Caulobacter crescentus / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D017319:Photosensitizing Agents / Q000032:analysis* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D008164:Luminescent Proteins; D017319:Photosensitizing Agents
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