Method for visualizing aerosol contamination in flow sorters.
The routine operation of a flow cytometer generates aerosols, and most commercially available instrument are not designed to be 100% effective in their elimination. Aerosols are a concern for operators as well as for others in the facility because of the potential for exposure to contamination, especially during sorting of biohazardous samples. This unit describes a visual method for examining aerosol containment using a commercial resin that fluoresces under UV or black light illumination. The method is rapid, inexpensive, and simple. Results are immediate and potentially quantitative.
Oberyszyn
Andrew S
AS
The Ohio State University Comprehensive Cancer Center, Analytical Cytometry Shared Resource Laboratory, Ohio State University, Columbus, Ohio, USA.
D000336:Aerosols; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design / Q000379:methods; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D015386:Hazardous Substances / Q000032:analysis; D008027:Light; D015203:Reproducibility of Results; D012449:Safety; D014466:Ultraviolet Rays
D000336:Aerosols; D015386:Hazardous Substances