The intracellular uptake ability of chitosan-coated Poly (D,L-lactide-co-glycolide) nanoparticles.
In this study, we prepared chitosan-coated Poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles. Specifically, we utilized a double emulsion-solvent evaporation technique to formulate nanoparticles containing paclitaxel as a model macromolecule and 6-coumarin as a fluorescent marker. SEM images verified that all nanoparticles were spherical in shape with smooth surfaces. Chitosan coating slightly increased the size distribution of the PLGA/PVA nanoparticles, from 202.2+/-3.2 nm to 212.2+/-2.9 nm, but the encapsulation efficiency was not significantly different. In contrast, coating with chitosan slowed the in vitro drug release rate and significantly changed the zeta potential from negative (-30.1+/-0.6 mV) to positive (26+/-1.2 mV). At the initial burst time, the drug release rate from chitosancoated nanoparticles was slightly slower than that of the uncoated nanoparticles. Chitosan-coated nanoparticles were also taken up much more efficiently than uncoated nanoparticles. This study demonstrated the efficacy of chitosancoated PLGA nanoparticles as an efficient delivery system.
Kim
Beom-Su
BS
Laboratory of Enzyme Technology, College of Natural Science, Chonbuk National University, Chonju 561-756, Korea.
Kim
Cheol-Sang
CS
Lee
Kang-Min
KM
D000269:Adhesives; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D048271:Chitosan; D003692:Delayed-Action Preparations; D004563:Electrochemistry; D005079:Excipients; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D017239:Paclitaxel / Q000008:administration & dosage / Q000494:pharmacology; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
D000269:Adhesives; D000972:Antineoplastic Agents, Phytogenic; D003692:Delayed-Action Preparations; D005079:Excipients; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan; D017239:Paclitaxel