m7GTP alphaS is a strong and stable inhibitor of cap-dependent translation.
Two diastereomers of 7-methylguanosine 5'-O-(1-thiotriphosphate) have been synthesized and resolved by RP HPLC. Preliminary studies revealed that these new analogs of mRNA cap are characterized by high affinity for eIF4E, resistance towards DcpS pyrophosphatase and high potency to inhibit cap-dependent translation.
Kowalska
Joanna
J
Division of Biophysics, Institute of Experimental Physics, University of Warsaw, 02-089 Warsaw, Poland.
Lukaszewicz
Maciej
M
Zuberek
Joanna
J
Ziemniak
Marcin
M
Strenkowska
Malwina
M
Darzynkiewicz
Edward
E
Jemielity
Jacek
J
D000818:Animals; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects*; D011500:Protein Synthesis Inhibitors / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D012314:RNA Cap Analogs / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D011817:Rabbits; D013237:Stereoisomerism
C541620:7-methylguanosine 5'-O-(1-thiotriphosphate); D039561:Eukaryotic Initiation Factor-4E; D011500:Protein Synthesis Inhibitors; D012314:RNA Cap Analogs; D006160:Guanosine Triphosphate