Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168.
Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.
Ohtani
Naoto
N
Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
Sato
Mitsuru
M
Tomita
Masaru
M
Itaya
Mitsuhiro
M
D001412:Bacillus subtilis / Q000235:genetics*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping
D004269:DNA, Bacterial; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific