title: Overview of functional cell assays.
abstract: Flow cytometry can measure a variety of functional parameters of increasing interest to cell biologists and clinicians. The distinguishing feature of these assays is that the information of interest is the functional behavior of the cell itself, rather than the phenotype. This unit discusses cell viability, oxidative metabolism, membrane potential, intracellular ionized calcium, intracellular pH, intracellular organelles, and gene reporter assays.
authors.affiliation: University of Washington, Seattle, Washington, USA.
authors.forename: Peter S
authors.initials: PS
authors.lastname: Rabinovitch
authors.forename: J Paul
authors.initials: JP
authors.lastname: Robinson
mesh_terms: D001681:Biological Assay / Q000379:methods*; D002118:Calcium / Q000378:metabolism; D002468:Cell Physiological Phenomena; D002470:Cell Survival; D005434:Flow Cytometry; D017930:Genes, Reporter; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000378:metabolism; D008564:Membrane Potentials; D015388:Organelles / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D002118:Calcium