Analysis of intracellular organelles by flow cytometry or microscopy.
Functional analysis of cellular organelles can be accomplished by staining cells with suitable organelle-specific dyes and analyzing the fluorescence of the stained cells with a flow cytometer. With this methodology it is possible to resolve suspected heterogeneity in organelle function or content within a population of cells. Morphological information can be provided by quantitative microscopy (confocal microscope or video microscope with digital image-analysis system). The thirteen protocols cover flow cytometry and microscopy for both live and fixed cells.
Poot
M
M
University of Washington, Seattle, Washington, USA.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D018613:Microscopy, Confocal / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D015388:Organelles / Q000737:chemistry*
D005456:Fluorescent Dyes