A new family of small (4kDa) neurotoxins from the venoms of spiders of the genus Phoneutria.
A family of 4kDa neurotoxic peptides was purified from venoms of Phoneutria spiders. All have six cysteine residues, and low similarity with other neurotoxins. Three toxins caused moderate inhibition of L-type Ca(2+) channels. The structure of toxin PRTx27C3 was modeled and compared with toxin ADO1. The importance of four residues is suggested.
Lúcio
A D
AD
Dept. of Biochemistry and Immunology; Instituto de Ciências Biológicas, Brazil.
Campos
F V
FV
Richardson
M
M
Cordeiro
M N
MN
Mazzoni
M S C
MS
de Lima
M E
ME
Pimenta
M C
MC
Bemquerer
M P
MP
Figueiredo
S G
SG
Gomes
P C
PC
Beirão
P S L
PS
D000595:Amino Acid Sequence; D000818:Animals; D002121:Calcium Channel Blockers / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D020746:Calcium Channels, L-Type / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009498:Neurotoxins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D021241:Spectrometry, Mass, Electrospray Ionization; D013111:Spider Venoms / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity; D013112:Spiders / Q000737:chemistry* / Q000235:genetics
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D009498:Neurotoxins; D013111:Spider Venoms