Effects of entomopathogenic bacterium Photorhabdus temperata infection on the digestive enzymes of Diatraea saccharalis (Lepidoptera: Crambidae) larvae.
This study investigated the effects of Photorhabdus temperata infection on the activities of digestive enzymes of the sugarcane stalk borer Diatraea saccharalis. Non-infected D. saccharalis larvae present a major alpha-amylase, several proteinases, three sucrose hydrolases and two alpha-glucosidases in their midgut. Analysis of these hydrolases by electrophoresis and "in gel" assays showed that the activities of all enzymes decreased following infection, with an initial decline observed 12 h after infection. The activities of alpha-glucosidases decreased by 50% twelve hours after infection, whereas, at this time, the alpha-galactosidase activities decreased by 70%. Interestingly, the animals died 48 h after infection, but approximately 5% of all the enzymes tested remained active in the midgut following host death. At this time, most of the cultivable native intestinal bacteria had died.
Carneiro
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Departamento de Bioquímica, Universidade Federal de Santa Catarina, CEP 88040-900, Florianópolis, Brasil. capsilva@ccb.ufsc.br.
Damatta
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Samuels
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Silva
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D000818:Animals; D004064:Digestive System / Q000201:enzymology / Q000382:microbiology; D006026:Glycoside Hydrolases / Q000378:metabolism; D054884:Host-Pathogen Interactions; D007814:Larva / Q000201:enzymology / Q000382:microbiology; D007915:Lepidoptera / Q000201:enzymology* / Q000382:microbiology*; D010447:Peptide Hydrolases / Q000378:metabolism; D020637:Photorhabdus / Q000472:pathogenicity*; D031786:Saccharum / Q000469:parasitology; D000516:alpha-Amylases / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
D006026:Glycoside Hydrolases; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D010447:Peptide Hydrolases