Analysis of cationic comb-type copolymers/DNA interaction by the single molecular observation and intermolecular force measurement.
We have reported that poly(L-lysine)-graft-dextran (PLL-g-Dex) accelerates DNA hybridization and increases stability of a double stranded DNA (dsDNA). Furthermore, PLL-g-Dex was found to stimulate the DNA strand exchange reaction between dsDNA and its complementary single stranded DNA (ssDNA). In order to better understand these phenomena, we evaluate PLL-g-Dex/DNA interaction by the single molecular observation and intermolecular force measurement at single molecular level. We have been able to observe PLL-g-Dex/DNA complex at single molecular level. PLL-g-Dex was found to increases the dissociation force of a dsDNA.
Hirano
Masanori
M
Institute for Materials Chemistry and Engineerng, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-8581, Japan.
Shimada
Naohiko
N
Kano
Arihiro
A
Kidoaki
Satoru
S
Maruyama
Atsushi
A
D004247:DNA / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry
D003911:Dextrans; C113090:poly(lysine)-graft-dextran; D011107:Polylysine; D004247:DNA