Expression of the CXCR6 on polymorphonuclear neutrophils in pancreatic carcinoma and in acute, localized bacterial infections.
The chemokine receptor CXCR6 has been described on lymphoid cells and is thought to participate in the homing of activated T-cells to non-lymphoid tissue. We now provide evidence that the chemokine receptor CXCR6 is also expressed by activated polymorphonuclear neutrophils (PMN) in vivo: Examination of biopsies derived from patients with pancreatic carcinoma by confocal laser scan microscopy revealed a massive infiltration of PMN that expressed CXCR6, while PMN of the peripheral blood of these patients did not. To answer the question whether CXCR6 expression is a property of infiltrated and activated PMN, leucocytes were collected from patients with localized soft tissue infections in the course of the wound debridement. By cytofluorometry, the majority of these cells were identified as PMN. Up to 50% of these PMN were also positive for CXCR6. Again, PMN from the peripheral blood of these patients were nearly negative for CXCR6, as were PMN of healthy donors. In a series of in vitro experiments, up-regulation of CXCR6 on PMN of healthy donors by a variety of cytokines was tested. So far, a minor, although reproducible, effect of tumour necrosis factor (TNFalpha) was seen: brief exposure with low-dose TNFalpha induced expression of CXCR6 on the surface of PMN. Furthermore, we could show an increased migration of PMN induced by the axis CXCL16 and CXCR6. In summary, our data provide evidence that CXCR6 is not constitutively expressed on PMN, but is up-regulated under inflammatory conditions and mediates migration of CXCR6-positive PMN.
Gaida
M M
MM
Department of Surgery, University of Heidelberg, Heidelberg, Germany.
Günther
F
F
Wagner
C
C
Friess
H
H
Giese
N A
NA
Schmidt
J
J
Hänsch
G M
GM
Wente
M N
MN
D000208:Acute Disease; D001424:Bacterial Infections / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D006801:Humans; D008024:Ligands; D018613:Microscopy, Confocal / Q000379:methods; D009363:Neoplasm Proteins / Q000378:metabolism*; D018375:Neutrophil Activation / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D010019:Osteomyelitis / Q000276:immunology; D010190:Pancreatic Neoplasms / Q000276:immunology*; D016459:Prosthesis-Related Infections / Q000276:immunology; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D018461:Soft Tissue Infections / Q000276:immunology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000276:immunology
C106330:CXCR6 protein, human; D008024:Ligands; D009363:Neoplasm Proteins; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine; D011991:Receptors, Virus
Shock. 2003 Dec;20(6):503-10
14625473
Cytokine Growth Factor Rev. 2005 Dec;16(6):593-609
16046180
Clin Lab. 2005;51(3-4):117-26
15819166
Mol Cancer Res. 2008 Apr;6(4):546-54
18344492
Cancer Sci. 2007 Nov;98(11):1652-8
17894551
Gut. 2002 Feb;50(2):150-2
11788551
J Infect Dis. 2004 Sep 1;190(5):989-97
15295707
Arch Immunol Ther Exp (Warsz). 2005 Nov-Dec;53(6):505-17
16407783
Immunology. 2007 May;121(1):1-14
17386080
J Immunol. 2004 May 15;172(10):6362-72
15128827
J Hepatol. 2003 Jan;38(1):67-75
12480562
Nat Rev Cancer. 2006 Jan;6(1):24-37
16397525
J Immunol. 2004 Jun 1;172(11):7136-43
15153537
J Immunol. 2005 Jan 15;174(2):1055-62
15634930
Br J Cancer. 2007 Oct 22;97(8):1106-15
17876328
J Leukoc Biol. 2006 Oct;80(4):682-4
16849612
J Immunol. 2005 Jan 1;174(1):277-83
15611250
Arthritis Rheum. 2005 Oct;52(10):3004-14
16200580
Clin Exp Allergy. 2005 Dec;35(12):1572-80
16393323
Blood. 2000 May 15;95(10):3032-43
10807766
Mediators Inflamm. 1998;7(1):19-23
9839694
Ann Rheum Dis. 2005 Oct;64(10):1436-42
15778239
N Engl J Med. 2001 Sep 13;345(11):833-5
11556308
J Hematother Stem Cell Res. 2001 Dec;10(6):739-48
11798500
J Clin Pathol. 2004 Jun;57(6):630-6
15166270
Cancer Lett. 2008 Feb 8;259(2):209-17
18054154
Am J Pathol. 2007 May;170(5):1485-96
17456756
Am J Respir Cell Mol Biol. 1999 Apr;20(4):769-76
10101010
Am J Respir Crit Care Med. 2005 Nov 15;172(10):1290-8
16100013
J Leukoc Biol. 2006 Oct;80(4):685-90
16864602
Clin Exp Immunol. 2006 Jan;143(1):70-7
16367936
Blood. 2005 Jul 15;106(2):419-27
15784733
Shock. 2004 Aug;22(2):108-15
15257082
Blood. 2006 Nov 1;108(9):2906-13
16849637
Cell Immunol. 2003 May;223(1):1-12
12914753
N Engl J Med. 2006 Feb 9;354(6):610-21
16467548
Ann N Y Acad Sci. 2006 Aug;1072:276-87
17057207
Eur J Clin Invest. 2004 Feb;34(2):149-55
14764079
Int J Oncol. 2002 Sep;21(3):649-54
12168113
Trends Immunol. 2004 Apr;25(4):201-9
15039047
J Immunol. 2004 Mar 15;172(6):3678-85
15004171
Pancreatology. 2005;5(6):514-29
16110250
Eur J Clin Invest. 1993 Nov;23(11):693-8
7905828