Construction, expression, purification and immunology effect of an anti-atherosclerosis chimeric enzyme vaccine in Escherichia coli.
In order to prevent atherosclerosis, a chimeric enzyme vaccine of AnsB-TTP-PADRE-CETPC was successfully constructed, expressed and purified to immunize New Zealand white rabbits for inducing high titers of anti-CETP antibodies to improve lipid abnormality. The protein was expressed as soluble protein in Escherichia coli and purified by anion exchange column and Sephadex G-100 size-exclusion chromatography. After immunizing rabbits with the purified protein, high titer anti-CETP antibodies were induced and lasted more than nineteen weeks in vivo; High density lipoprotein cholesterol (HDL-C) content in the serum was elevated to 61% while decreased low density lipoprotein cholesterol (LDL-C) to 37.2% compared with control rabbits in the presence of Al(OH) (3).
Yunxiao
Sun
S
Minigene Pharmacy Laboratory, Biopharmaceutical College, China Pharmaceutical University, Nanjing, People's Republic of China. Liujingj@public1.ptt.js.cn.
Zhufang
Li
L
Xin
Yang
Y
Jun
Long
L
Qiyan
Xiong
X
Gaofu
Qi
Q
Rongyue
Cao
C
Jie
Wu
W
Jingjing
Liu
L
Hao
Fan
F
Taiming
Li
L
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001215:Asparaginase / Q000235:genetics / Q000276:immunology; D050197:Atherosclerosis / Q000097:blood / Q000276:immunology* / Q000517:prevention & control*; D001483:Base Sequence; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D018985:Epitopes, B-Lymphocyte / Q000235:genetics; D018984:Epitopes, T-Lymphocyte / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008055:Lipids / Q000097:blood; D017780:Malaria Vaccines / Q000235:genetics / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D013744:Tetanus Toxin / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology
C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins; D017931:DNA Primers; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D008055:Lipids; D017780:Malaria Vaccines; C401223:PADRE 45; D010446:Peptide Fragments; D013744:Tetanus Toxin; D014614:Vaccines, Synthetic; D001215:Asparaginase