Hydroxysafflor yellow A protects neuron against hypoxia injury and suppresses inflammatory responses following focal ischemia reperfusion in rats.
Previous data demonstrated that hydroxysafflor yellow A (HSYA), a yellow color pigments extracted from the safflower, was an effective agent against focal cerebral ischemia. In the present study we demonstrated that HSYA prevented the injury in cultured cerebral cortical neurons induced by oxygen-glucose deprivation and increased the cell viability, as shown by the inhibition of both LDH and NO efflux. Further, HSYA administered orally 3 d before middle cerebral artery occlusion has the capacity to reduce cerebral infarct size and edema after 2 h cerebral ischemia followed by 24 h reperfusion in rats, and to significantly improve neurological behavior scores. Mean while, treatment with HSYA significantly decreased both mRNA and protein levels of IL-1beta, TNF-alpha in ischemic brain tissue. These results suggested that the protection of HSYA results from, at least in part, suppression of inflammatory responses following focal ischemia reperfusion.
Ye
Sheng-Ying
SY
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Gao
Wen-Yuan
WY
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D015153:Blotting, Western; D001929:Brain Edema / Q000473:pathology / Q000517:prevention & control; D015687:Cell Hypoxia / Q000187:drug effects*; D002478:Cells, Cultured; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000172:deficiency; D007202:Indicators and Reagents; D007249:Inflammation / Q000473:pathology* / Q000517:prevention & control*; D053583:Interleukin-1beta / Q000096:biosynthesis; D002546:Ischemic Attack, Transient / Q000473:pathology* / Q000517:prevention & control* / Q000523:psychology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents; D009569:Nitric Oxide / Q000378:metabolism; D011247:Pregnancy; D011809:Quinones / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology* / Q000517:prevention & control*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D007202:Indicators and Reagents; D053583:Interleukin-1beta; D018696:Neuroprotective Agents; D011809:Quinones; D014409:Tumor Necrosis Factor-alpha; C085278:hydroxysafflor yellow A; D009569:Nitric Oxide; D002599:Chalcone; D007770:L-Lactate Dehydrogenase; D005947:Glucose