title: Splenic CD8+ T cells secrete TGF-beta1 to exert suppression in mice with anterior chamber-associated immune deviation.
abstract: Background CD8+ regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8+ T cells in ACAID remain only poorly understood. TGF-beta1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-beta1 by CD8+ T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-beta1, is unknown. Methods The suppressive effect of CD8+ T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-beta1 by CD8+ T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-beta1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8+ T cells. Results CD8+ T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-beta1, and their suppression could partially be blocked by anti-TGF-beta1 antibodies. Conclusions Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-beta1.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangzhou, People's Republic of China.
authors.forename: Liqiong
authors.initials: L
authors.lastname: Jiang
authors.forename: Hao
authors.initials: H
authors.lastname: He
authors.forename: Peizeng
authors.initials: P
authors.lastname: Yang
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Lin
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhou
authors.forename: Xiangkun
authors.initials: X
authors.lastname: Huang
authors.forename: Aize
authors.initials: A
authors.lastname: Kijlstra
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000867:Anterior Chamber / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000378:metabolism; D007108:Immune Tolerance / Q000276:immunology*; D016753:Interleukin-10 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D053773:Transforming Growth Factor beta1; D016753:Interleukin-10
references.citation: J Immunol. 2005 Mar 15;174(6):3247-55
references.pmid: 15749855
references.citation: Annu Rev Immunol. 1998;16:137-61
references.pmid: 9597127
references.citation: Cell Mol Immunol. 2005 Feb;2(1):11-9
references.pmid: 16212906
references.citation: Int Rev Immunol. 2002 Mar-Jun;21(2-3):123-52
references.pmid: 12424840
references.citation: Immunology. 2007 Jan;120(1):112-9
references.pmid: 17052246
references.citation: J Immunol. 2004 May 1;172(9):5213-21
references.pmid: 15100259
references.citation: Annu Rev Immunol. 1994;12:809-37
references.pmid: 8011298
references.citation: Invest Ophthalmol Vis Sci. 2003 Dec;44(12):5242-51
references.pmid: 14638723
references.citation: J Immunol. 1998 Nov 15;161(10):5382-90
references.pmid: 9820512
references.citation: J Immunol. 1994 Oct 1;153(7):2962-73
references.pmid: 8089480
references.citation: J Immunol. 2004 May 1;172(9):5149-53
references.pmid: 15100250
references.citation: Blood. 2003 Dec 1;102(12):4107-14
references.pmid: 12893750
references.citation: J Immunol. 2005 Jun 15;174(12):7625-32
references.pmid: 15944263
references.citation: J Immunol. 2006 Nov 1;177(9):6091-7
references.pmid: 17056535
references.citation: J Immunol. 2006 Dec 15;177(12):8331-7
references.pmid: 17142729
references.citation: Cell. 2000 May 26;101(5):455-8
references.pmid: 10850488
references.citation: Immunology. 1990 Nov;71(3):383-9
references.pmid: 1702748
references.citation: J Immunol. 2005 Dec 1;175(11):7728-37
references.pmid: 16301683
references.citation: J Immunol. 1998 Mar 1;160(5):2089-98
references.pmid: 9498745
references.citation: J Exp Med. 2002 Mar 18;195(6):695-704
references.pmid: 11901196
references.citation: Invest Ophthalmol Vis Sci. 2000 Jun;41(7):1803-11
references.pmid: 10845601
references.citation: Ocul Immunol Inflamm. 2003 Mar;11(1):39-52
references.pmid: 12854026
references.citation: J Immunol. 2005 Dec 1;175(11):7093-7
references.pmid: 16301610
references.citation: J Immunol. 2003 Aug 1;171(3):1266-71
references.pmid: 12874214
references.citation: Invest Ophthalmol Vis Sci. 2000 May;41(6):1410-21
references.pmid: 10798657
references.citation: Int Immunol. 2006 Nov;18(11):1549-62
references.pmid: 16966495
references.citation: J Immunol. 2001 Oct 15;167(8):4245-53
references.pmid: 11591746
references.citation: Mol Vis. 2007 Jun 19;13:968-74
references.pmid: 17653037
references.citation: J Exp Med. 2005 Apr 4;201(7):1061-7
references.pmid: 15809351
references.citation: J Exp Med. 2003 Dec 15;198(12):1875-86
references.pmid: 14676299
references.citation: Eur J Immunol. 2006 Feb;36(2):397-410
references.pmid: 16402405
references.citation: Annu Rev Immunol. 2006;24:99-146
references.pmid: 16551245
references.citation: J Immunol. 2001 Apr 1;166(7):4327-33
references.pmid: 11254685