title: The effect of blastomere biopsy on preimplantation mouse embryo development and global gene expression.
abstract: The blastomere biopsy procedure does not affect preimplantation embryo development or global patterns of gene expression in a mouse model of Preimplantation Genetic Testing (PGT). However, zona breaching, which is inherent to the blastomere biopsy procedure, causes significant premature and sometimes abnormal hatching.
authors.affiliation: Center for Research on Reproduction and Women's Health, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Francesca E
authors.initials: FE
authors.lastname: Duncan
authors.forename: Paula
authors.initials: P
authors.lastname: Stein
authors.forename: Carmen J
authors.initials: CJ
authors.lastname: Williams
authors.forename: Richard M
authors.initials: RM
authors.lastname: Schultz
mesh_terms: D000818:Animals; D001706:Biopsy; D001757:Blastomeres / Q000473:pathology*; D004624:Embryo Transfer / Q000379:methods*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D023421:Models, Animal; D019836:Preimplantation Diagnosis / Q000379:methods; D020554:Sperm Injections, Intracytoplasmic; D013997:Time Factors
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