Clonal relationship among Vibrio cholerae O1 El Tor strains isolated in Somalia.
One hundred and three Vibrio cholerae O1 strains, selected to represent the cholera outbreaks which occurred in Somalia in 1998-1999, were characterized by random amplified polymorphic DNA patterns, ribotyping, and antimicrobial susceptibility. All strains showed a unique amplified DNA pattern and 2 closely related ribotypes (B5a and B8a), among which B5a was the more frequently identified. Ninety-one strains were resistant to ampicillin, chloramphenicol, spectinomycin, streptomycin, sulfamethoxazole, and trimethoprim, conferred, except for spectinomycin, by a conjugative plasmid IncC. These findings indicated that the group of strains active in Somalia in the late 1990s had a clonal origin.
Scrascia
Maria
M
Dipartimento di Genetica e Microbiologia, Università di Bari, Italy.
Pugliese
Nicola
N
Maimone
Francesco
F
Mohamud
Kadigia A
KA
Grimont
Patrick A D
PA
Materu
Sadiki F
SF
Pazzani
Carlo
C
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D016000:Cluster Analysis; D003227:Conjugation, Genetic; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids; D019105:Random Amplified Polymorphic DNA Technique; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D012998:Somalia / Q000453:epidemiology; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial