TGFA/TAQ I polymorphism in nonsyndromic cleft lip and palate patients from Rio Grande Do Sul, Brazil.
OBJECTIVE
To test the TGFA/Taq I polymorphism in the development of nonsyndromic cleft lip and palate.


METHODS
The research was based on a case-control study, including nonsyndromic cleft lip and palate patients (140 individuals) and a control sample of unaffected individuals (142) to ascertain the absence or presence of genic mutation at the TGFA locus.


METHODS
The DNA of carriers of nonsyndromic cleft lip with or without cleft palate was obtained by buccal swab, and the DNA of the control group was extracted from peripheral blood leucocytes. TGFA/Taq I polymorphism was determined genetically by polymerase chain reaction using specific primers and fragment digestion with Taq I restriction enzyme.


RESULTS
No significant association was detected when patients and controls were compared with the genotype for TGFA/Taq I polymorphism.


CONCLUSIONS
Mutations in TGFA gene have no association with nonsyndromic cleft lip and palate in the sample from Rio Grande do Sul. Therefore, based on this study, it is not possible to determine the role played by TGFA in the expression of cleft lip and palate.
Ehlers Bertoja
Angela
A
Odontology (Buccomaxilofacial Surgery and Traumatology) at the Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil. angelabertoja@hotmail.com
Sampaio Alho
Clarice
C
De França
Everaldo
E
Menegotto
Beatriz
B
Miriam Robinson
Wanyce
W
D000225:Adenine; D000293:Adolescent; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007438:Introns / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D017384:Sequence Deletion / Q000235:genetics; D019914:Taq Polymerase / Q000235:genetics; D013941:Thymine; D016211:Transforming Growth Factor alpha / Q000235:genetics*; D055815:Young Adult
D016211:Transforming Growth Factor alpha; D004247:DNA; D019914:Taq Polymerase; D000225:Adenine; D013941:Thymine