title: Immunohistochemical localization of glycogen phosphorylase isozymes in the rat gastrointestinal muscle layers and enteric nervous system.
abstract: Glycogen represents the major brain energy reserve though its precise functions are still under debate. Glycogen has also been found in different cell types of the enteric nervous system (ENS), the largest and most complex component of the peripheral nervous system. In the present work we have demonstrated, by application of isozyme-specific antibodies, the presence of isozymes of glycogen phosphorylase (GP), one of the major control sites in glycogen metabolism, in the rat ENS. Immunohistochemistry revealed that isoform BB (brain) is the predominant isozyme expressed in enteric glial cells (EGC) and rare neurons of the myenteric and submucosal plexuses. Isoform MM (muscle) appears in cells which are, according to their location and morphology, probably interstitial cells of Cajal (ICC). In addition, both GP isoforms are expressed in longitudinal and circular intestinal smooth muscle layers. As GP BB is mainly regulated by the cellular AMP level, a special function of glycogen in the energy supply of neural gut functions is suggested.
authors.affiliation: Interfaculty Institute for Biochemistry, University of Tuebingen, Tuebingen, Germany.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Pfeiffer-Guglielmi
authors.forename: Mike
authors.initials: M
authors.lastname: Francke
authors.forename: Christian
authors.initials: C
authors.lastname: Roski
authors.forename: Menachem
authors.initials: M
authors.lastname: Hanani
authors.forename: Andreas
authors.initials: A
authors.lastname: Reichenbach
authors.forename: Bernd
authors.initials: B
authors.lastname: Hamprecht
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017615:Enteric Nervous System / Q000201:enzymology*; D005260:Female; D041981:Gastrointestinal Tract / Q000201:enzymology* / Q000294:innervation; D025002:Glycogen Phosphorylase, Brain Form / Q000378:metabolism*; D024982:Glycogen Phosphorylase, Muscle Form / Q000378:metabolism*; D007150:Immunohistochemistry; D007420:Intestine, Large / Q000201:enzymology / Q000294:innervation; D007421:Intestine, Small / Q000201:enzymology / Q000294:innervation; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D009130:Muscle, Smooth / Q000201:enzymology*; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000201:enzymology / Q000294:innervation
chemical_list: D007527:Isoenzymes; D025002:Glycogen Phosphorylase, Brain Form; D024982:Glycogen Phosphorylase, Muscle Form
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