title: Resistance to Magnaporthe grisea in transgenic rice with suppressed expression of genes encoding allene oxide cyclase and phytodienoic acid reductase.
abstract: Linolenic acid (18:3) and its derivative jasmonic acid (JA) are important molecules in disease resistance in many dicotyledonous plants. We have previously used 18:3- and JA-deficient rice (F78Ri) to investigate the roles of fatty acids and their derivatives in resistance to the blast fungus Magnaporthe grisea [A. Yara, T. Yaeno, J.-L. Montillet, M. Hasegawa, S. Seo, K. Kusumi, K. Iba, Enhancement of disease resistance to Magnaporthe grisea in rice by accumulation of hydroxy linoleic acid, Biochem. Biophys. Res. Commun. 370 (2008) 344-347; A. Yara, T. Yaeno, M. Hasegawa, H. Seto, J.-L. Montillet, K. Kusumi, S. Seo, K. Iba, Disease resistance against Magnaporthe grisea is enhanced in transgenic rice with suppression of omega-3 fatty acid desaturases, Plant Cell Physiol. 48 (2007) 1263-1274]. However, because F78Ri plants are suppressed in the first step of the JA biosynthetic pathway, we could not confirm the specific contribution of JA to disease resistance. In this paper, we generated two JA-deficient rice lines (AOCRi and OPRRi) with suppressed expression of the genes encoding allene oxide cyclase (AOC) and 12-oxo-phytodienoic acid reductase (OPR), which catalyze late steps in the JA biosynthetic pathway. The levels of disease resistance in the AOCRi and OPRRi lines were equal to that in wild-type plants. Our data suggest that resistance to M. grisea is not dependent on JA synthesis.
authors.affiliation: Department of Biology, Faculty of Sciences, Kyushu University, 6-10-1 Hakozaki, Higash-ku, Fukuoka 812-8581, Japan.
authors.forename: Asanori
authors.initials: A
authors.lastname: Yara
authors.forename: Takashi
authors.initials: T
authors.lastname: Yaeno
authors.forename: Morifumi
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Hideharu
authors.initials: H
authors.lastname: Seto
authors.forename: Shigemi
authors.initials: S
authors.lastname: Seo
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kusumi
authors.forename: Koh
authors.initials: K
authors.lastname: Iba
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D020082:Magnaporthe; D012275:Oryza / Q000235:genetics / Q000382:microbiology*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics*; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000382:microbiology
chemical_list: D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D054883:Oxylipins; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; D044925:Oxidoreductases Acting on CH-CH Group Donors; C106360:12-oxophytodienoate reductase; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase