Characterisation of lipoxygenase isoforms from olive callus cultures.
Two lipoxygenase isoforms from olive callus cultures were separated from each other. Acetone powders were made to stabilise activity and remove lipids. Separation was then achieved by salt precipitation and ion-exchange chromatography. Both isoforms had comparable activity with linoleic and alpha-linolenic acid substrates, a basic pH optimum and had molecular masses of around 95 kDa. The callus extracts preferentially formed the 13-hydroperoxy products, in keeping with the pattern of volatile derivatives found in olive tissues and oils derived therefrom.
Williams
Mark
M
School of Biosciences, Cardiff University, Cardiff CF10 3AX, Wales, UK.
Harwood
John L
JL
D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes; D019787:Linoleic Acid / Q000378:metabolism; D008084:Lipoxygenase / Q000302:isolation & purification* / Q000378:metabolism*; D008970:Molecular Weight; D031658:Olea / Q000201:enzymology*; D013379:Substrate Specificity; D017962:alpha-Linolenic Acid / Q000378:metabolism
D007527:Isoenzymes; D017962:alpha-Linolenic Acid; D019787:Linoleic Acid; D008084:Lipoxygenase