The alpha-N-acetyl-glucosaminidase gene is transcriptionally activated in male and female gametes prior to fertilization and is essential for seed development in Arabidopsis.
Sugar residues in proteoglycan complexes carry important signalling and regulatory functions in biology. In humans, heparan sulphate is an example of such a complex polymer containing glucosamine and N-acetyl-glucosamine residues and is present in the extracellular matrix. Although heparan sulphate has not been found in plants, the At5g13690 gene encoding the alpha-N-acetyl-glucosaminidase (NAGLU), an enzyme involved in its catabolism, is present in the Arabidopsis genome. Among our collection of embryo-defective lines, a plant was identified in which the T-DNA had inserted into the AtNAGLU gene. The phenotype of atnaglu is an early arrest of seed development without apparent male or female gametophytic effects. These data demonstrated the essential function in Arabidopsis consistent with the contribution of NAGLU to the Sanfilippo syndrome in human. Expression of AtNAGLU in plants was shown to be prevalent during reproductive development. The presence of AtNAGLU mRNA was observed during early and late male gametogenesis and in each cell of the embryo sac at the time of fertilization. After fertilization, AtNAGLU was expressed in the embryo, suspensor, and endosperm until the cotyledonary stage embryo. This precise pattern of expression identifies the cells and tissues where a remodelling of the N-acetyl-glucosamine residues of proteoglycan complexes is occurring. This work provides original evidence of the important role of N-acetyl-glucosamines in plant reproductive development.
Ronceret
Arnaud
A
Laboratoire Génome et Développement des Plantes, UMR-CNRS-IRD-Université 5096, 52 Avenue Paul Alduy, F-66860 Perpignan-cedex, France.
Gadea-Vacas
Jose
J
Guilleminot
Jocelyne
J
Devic
Martine
M
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005854:Germ Cells / Q000737:chemistry / Q000201:enzymology* / Q000254:growth & development; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D011134:Polysaccharides / Q000378:metabolism; D012098:Reproduction; D012639:Seeds / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D016415:Sequence Alignment; D014158:Transcription, Genetic; D048809:alpha-N-Acetylgalactosaminidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D011134:Polysaccharides; D048809:alpha-N-Acetylgalactosaminidase