title: Platelet storage in Fresenius/NPBI polyolefin and BTHC-PVC bags: a direct comparison.
abstract: New platelet storage systems, such as changes in the plastic of the storage bags, require validation. In this study, pooled buffy coat platelets stored in Fresenius/NPBI polyolefin bags were compared with those stored in Fresenius/NPBI butyryl-trihexyl citrate (BTHC) plasticized polyvinyl chloride (PVC). The CompoSelect thrombocyte polishing filter system (1000 mL polyolefin bag) and the CompoStop F730 system (1300 mL BTHC-PVC bag) were used to prepare paired, plasma-suspended, buffy coat platelet concentrates. Samples were taken up to day 7 for in vitro analysis. In a separate experiment, 12 units were prepared using the CompoStop F730 system and samples taken after leucofiltration for FXIIa assay. By day 7, platelet concentrates stored in BTHC-PVC demonstrated significantly higher pH levels (7.32 +/- 0.05 vs. 7.26 +/- 0.05) and a greater degree of cell lysis as shown by increased lactate dehydrogenase levels (497 +/- 107 vs. 392 +/- 81 U L(-1)). The supernatants contained higher concentrations of soluble P-selectin and the chemokine 'regulated on activation, normal T-cell expressed and presumably secreted', which are released from the alpha-granules during activation. The ATP concentrations were significantly lower in BTHC-PVC. Platelet counts, mean platelet volume and hypotonic shock response were similar for both bags. FXIIa antigen concentrations were 0.6 +/- 0.2 ng mL(-1) indicating that activation of the contact factor pathway had not occurred. Although the CompoStop F730 leucoreduction filter did not activate the contact system, platelets stored in 100% plasma in BTHC-PVC bags demonstrated different in vitro characteristics from those stored in polyolefin. Further work is required to demonstrate whether these differences will affect in vivo recovery and survival.
authors.affiliation: Products and Components R&D Group, National Science Laboratory, Scottish National Blood Transfusion Service, Edinburgh, UK. valerie.hornsey@snbts.csa.scot.nhs.uk
authors.forename: V S
authors.initials: VS
authors.lastname: Hornsey
authors.forename: K
authors.initials: K
authors.lastname: McColl
authors.forename: O
authors.initials: O
authors.lastname: Drummond
authors.forename: I R
authors.initials: IR
authors.lastname: Macgregor
authors.forename: C V
authors.initials: CV
authors.lastname: Prowse
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D002087:Butyrates; D002245:Carbon Dioxide / Q000378:metabolism*; D006801:Humans; D010100:Oxygen / Q000378:metabolism*; D011090:Polyenes; D011143:Polyvinyl Chloride; D019064:Product Packaging; D013997:Time Factors
chemical_list: D002087:Butyrates; D011090:Polyenes; D002245:Carbon Dioxide; C066757:butyryl-n-trihexylcitrate; C035051:PL 732; D011143:Polyvinyl Chloride; D010100:Oxygen