title: Protein-facilitated ribozyme folding and catalysis.
abstract: In vivo, large RNAs rely on proteins to fold to their native conformation. In the case of the S. cerevisiae group II intron ai5 gamma, the DEAD-box protein Mss116 has been shown to promote the formation of the catalytically active structure. However, it is a matter of debate whether it does this by stabilizing on-pathway intermediates or by disrupting misfolded structures. Here we present the available experimental evidence to distinguish between those mechanisms and discuss the possible interpretations.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA. nora.zingler@yale.edu
authors.forename: Nora
authors.initials: N
authors.lastname: Zingler
authors.forename: Amanda
authors.initials: A
authors.lastname: Solem
authors.forename: Anna Marie
authors.initials: AM
authors.lastname: Pyle
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D016337:RNA, Catalytic / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D016337:RNA, Catalytic; D029701:Saccharomyces cerevisiae Proteins; C058150:MSS116 protein, S cerevisiae; D053487:DEAD-box RNA Helicases