title: Analysis of acamprosate in beagle dog plasma by LC-MS-MS.
abstract: A rapid, sensitive, and specific analytical method was developed and validated to quantify acamprosate calcium in beagle dog plasma. The method employs a single plasma protein precipitation, and the analytes are separated by chromatography on an Acquity UPLC HSS T3 column and analyzed by mass spectrometry in the multiple reaction monitoring (MRM) mode. The method has a chromatographic run time of 1.25 min and a linear calibration curve over the range 200-10000 ng/mL (r(2)>0.9994). The intra-day and inter-day accuracy and precision were within 10.0% for the analyte. Acamprosate was stable during all sample storage, preparation, and analytical periods. This method was employed in a pharmacokinetic study of an acamprosate 333 mg enteric-coated tablet in 8 male beagle dogs that received single 666 mg doses (333 mg x 2 tablets). The proposed method enables identification and quantification in pharmacokinetic studies of acamprosate in beagle dog plasma.
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Yun-Seok
authors.initials: YS
authors.lastname: Rhee
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Park
authors.forename: Seok
authors.initials: S
authors.lastname: Park
authors.forename: Chun-Woong
authors.initials: CW
authors.lastname: Park
authors.forename: Jeong-Myung
authors.initials: JM
authors.lastname: Ha
authors.forename: Ki-Woo
authors.initials: KW
authors.lastname: Jeong
authors.forename: Dong-Soo
authors.initials: DS
authors.lastname: Lee
authors.forename: Eun-Seok
authors.initials: ES
authors.lastname: Park
mesh_terms: D000077443:Acamprosate; D000427:Alcohol Deterrents / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004285:Dogs; D005615:Freezing; D008297:Male; D013058:Mass Spectrometry; D011786:Quality Control; D012015:Reference Standards; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization; D013608:Tablets, Enteric-Coated; D013654:Taurine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
chemical_list: D000427:Alcohol Deterrents; D012996:Solutions; D013608:Tablets, Enteric-Coated; D013654:Taurine; D000077443:Acamprosate