Hydrophobic thickness, lipid surface area and polar region hydration in monounsaturated diacylphosphatidylcholine bilayers: SANS study of effects of cholesterol and beta-sitosterol in unilamellar vesicles.
The influence of a mammalian sterol cholesterol and a plant sterol beta-sitosterol on the structural parameters and hydration of bilayers in unilamellar vesicles made of monounsaturated diacylphosphatidylcholines (diCn:1PC, n=14-22 is the even number of acyl chain carbons) was studied at 30 degrees C using small-angle neutron scattering (SANS). Recently published advanced model of lipid bilayer as a three-strip structure was used with a triangular shape of polar head group probability distribution (Kucerka et al., Models to analyze small-angle neutron scattering from unilamellar lipid vesicles, Physical Review E 69 (2004) Art. No. 051903). It was found that 33 mol% of both sterols increased the thickness of diCn:1PC bilayers with n=18-22 similarly. beta-sitosterol increased the thickness of diC14:1PC and diC16:1PC bilayers a little more than cholesterol. Both sterols increased the surface area per unit cell by cca 12 A(2) and the number of water molecules located in the head group region by cca 4 molecules, irrespective to the acyl chain length of diCn:1PC. The structural difference in the side chain between cholesterol and beta-sitosterol plays a negligible role in influencing the structural parameters of bilayers studied.
Gallová
J
J
Department of Physical Chemistry of Drugs, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, Slovakia. jana.gallova@fpharm.uniba.sk
Uhríková
D
D
Kucerka
N
N
Teixeira
J
J
Balgavý
P
P
D002784:Cholesterol / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry*; D033363:Neutron Diffraction; D010713:Phosphatidylcholines / Q000737:chemistry; D053838:Scattering, Small Angle; D012855:Sitosterols / Q000737:chemistry*; D013499:Surface Properties; D053835:Unilamellar Liposomes / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D012855:Sitosterols; D053835:Unilamellar Liposomes; D014867:Water; C025473:gamma-sitosterol; D002784:Cholesterol