title: Laboratory maintenance of Borrelia burgdorferi.
abstract: This unit describes the propagation of Borrelia burgdorferi as well as other "cultivable" Borrelia species in Barbour-Stoenner-Kelly-II (BSK-II) medium. It supplies a detailed recipe for BSK-II, as well as protocols for standard liquid culture, the generation of frozen Borrelia stocks, and the plating of B. burgdorferi cells in solid BSK-II medium.
authors.affiliation: University of Kansas Medical Center, Kansas City, Kansas, USA.
authors.forename: Wolfram R
authors.initials: WR
authors.lastname: Zückert
mesh_terms: D001431:Bacteriological Techniques; D025065:Borrelia burgdorferi / Q000254:growth & development*; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans
chemical_list: D003470:Culture Media