title: Microscope objectives.
abstract: The objective is the most crucial image-forming component of a microscope. A knowledge of the many types of objectives available and their characteristics is critical to the selection of appropriate objectives for image cytometry. This unit discusses aberrations in image formation and their correction, construction and types of objectives, and objectives for other microscopy applications, explaining the advantages and limitations of each one.
authors.affiliation: Olympus America Inc., Melville, New York, USA.
authors.forename: M
authors.initials: M
authors.lastname: Abramowitz
authors.forename: M M
authors.initials: MM
authors.lastname: Friedman
mesh_terms: D000818:Animals; D004867:Equipment Design; D006801:Humans; D007259:Infrared Rays; D008027:Light; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000379:methods; D008860:Microscopy, Ultraviolet / Q000379:methods; D055585:Physical Phenomena; D010825:Physics; D015203:Reproducibility of Results