Interactions between antitumor drugs and vault RNA.
It is supposed that ribonucleoprotein particle vault is involved in detoxification processes and thus is related to multidrug resistance. The vault is composed of three proteins and three vault RNAs, hvg-1, -2 and -3. The direct interactions between vault components and drugs were not reported. Recently, we revealed the interactions between vault RNAs and mitoxantrone. Here, we examined the interactions between hvg-2 and six antitumor drugs by a chemical shift perturbation method of NMR. It was found that in addition to mitoxantrone, hvg-2 can interact with two drugs basically in the same way using the same site. The difference in the affinity was also noticed among three drugs. Hvg-2 did not bind to the other three drugs. It is suggested that the common or closely related chemical structure of the positive three drugs is recognized by vault RNA.
Mashima
Tsukasa
T
Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Kudo
Michiko
M
Takada
Yusuke
Y
Matsugami
Akimasa
A
Gopinath
Subash C B
SC
Kumar
Penmetcha K R
PK
Katahira
Masato
M
D000970:Antineoplastic Agents / Q000737:chemistry*; D001483:Base Sequence; D008942:Mitoxantrone / Q000737:chemistry; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D012313:RNA / Q000737:chemistry*; D020394:Vault Ribonucleoprotein Particles / Q000737:chemistry*
D000970:Antineoplastic Agents; D020394:Vault Ribonucleoprotein Particles; D012313:RNA; D008942:Mitoxantrone