title: Molecular types and genetic profiles of Staphylococcus aureus strains isolated from bovine intramammary infections and extramammary sites.
abstract: Staphylococcus aureus isolates collected from sites of intramammary infection during a 10-month period and from extramammary sites (dairy cow teat skin, teat canals, and skin lesions; milking liners; and hands and nostrils of milking personnel) at two separately managed Finnish dairy herd establishments were analyzed to study the sources and reservoirs of bovine S. aureus intramammary infection. Selected isolates were subjected to pulsed-field gel electrophoresis (PFGE) typing and PCR analysis for genes encoding hemolysins (hla to hlg), leukocidins (lukED and lukM), superantigens (sea, sec, sed, seg to seo, seu, and tst), adhesins (fnbA and fnbB), and penicillin and methicillin resistance (blaZ and mecA). S. aureus was found throughout the herds in 94% of the cows. Nine PFGE types were found, with the herds each having their own predominant type and sharing one type. The degree of diversity of PFGE types in herd II, which integrated foreign heifers, was higher than that in herd I. For both herds, the majority of the PFGE-typed isolates both from milk and from extramammary sites represented the predominant PFGE types. In isolates from herd I, the most prevalent genes were hla-hlg, lukED, and fnbA; in those from herd II, they were hla, hld, hlg, lukED, and fnbA. The other genes were pulsotype linked within the herds. The predominant PFGE types carried both fnbA and fnbB; only fnbA was detected in the other PFGE types. No connection between specific virulence genes and the origins of isolates was found. The results suggest that for the two herds, most S. aureus isolates from extramammary sites were indistinguishable from the isolates infecting the mammary gland and that those sites can thus act as origins and reservoirs of intramammary infections. However, contamination in the opposite direction cannot be excluded.
authors.affiliation: Department of Production Animal Medicine, Faculty of Veterinary Medicine, P.O. Box 57, 00014 University of Helsinki, Finland. maarit.haveri@helsinki.fi
authors.forename: M
authors.initials: M
authors.lastname: Haveri
authors.forename: M
authors.initials: M
authors.lastname: Hovinen
authors.forename: A
authors.initials: A
authors.lastname: Roslöf
authors.forename: S
authors.initials: S
authors.lastname: Pyörälä
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002417:Cattle; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004783:Environmental Microbiology; D005838:Genotype; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013207:Staphylococcal Skin Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D037521:Virulence Factors
references.citation: J Med Microbiol. 2005 Apr;54(Pt 4):401-411
references.pmid: 15770028
references.citation: Clin Infect Dis. 1999 Nov;29(5):1128-32
references.pmid: 10524952
references.citation: Epidemiol Infect. 2002 Oct;129(2):387-95
references.pmid: 12403115
references.citation: J Dairy Sci. 1994 Feb;77(2):446-52
references.pmid: 8182169
references.citation: Microbiology (Reading). 2008 Jul;154(Pt 7):1949-1959
references.pmid: 18599823
references.citation: Infect Immun. 1999 Sep;67(9):4673-8
references.pmid: 10456915
references.citation: Annu Rev Microbiol. 1994;48:585-617
references.pmid: 7826020
references.citation: J Lab Clin Med. 2000 Mar;135(3):225-30
references.pmid: 10711860
references.citation: J Dairy Sci. 2006 Jul;89(7):2542-51
references.pmid: 16772573
references.citation: Trends Microbiol. 2001 Nov;9(11):547-53
references.pmid: 11825715
references.citation: Infect Immun. 1998 Nov;66(11):5238-43
references.pmid: 9784528
references.citation: Vet Microbiol. 2007 Nov 15;125(1-2):128-40
references.pmid: 17614219
references.citation: J Clin Microbiol. 2002 Nov;40(11):3894-902
references.pmid: 12409348
references.citation: J Clin Microbiol. 2005 Mar;43(3):1278-84
references.pmid: 15750096
references.citation: Eur J Clin Microbiol Infect Dis. 2000 Feb;19(2):101-7
references.pmid: 10746495
references.citation: Acta Vet Scand. 1999;40(3):279-86
references.pmid: 10605145
references.citation: J Dairy Sci. 1994 Nov;77(11):3354-64
references.pmid: 7814712
references.citation: J Dairy Sci. 2004 Aug;87(8):2433-41
references.pmid: 15328265
references.citation: APMIS. 1999 Apr;107(4):425-30
references.pmid: 10230698
references.citation: J Dairy Sci. 2000 Feb;83(2):278-84
references.pmid: 10714861
references.citation: J Dairy Sci. 2006 Mar;89(3):1010-23
references.pmid: 16507696
references.citation: J Clin Microbiol. 2000 Mar;38(3):1032-5
references.pmid: 10698991
references.citation: Lett Appl Microbiol. 2004;38(6):493-8
references.pmid: 15130145
references.citation: PLoS One. 2007 Oct 31;2(10):e1120
references.pmid: 17971880
references.citation: Acta Vet Scand. 2003;44(1-2):53-62
references.pmid: 14650544
references.citation: FEMS Microbiol Lett. 2004 Apr 1;233(1):45-52
references.pmid: 15043868
references.citation: Antimicrob Agents Chemother. 1998 Nov;42(11):2817-23
references.pmid: 9797209
references.citation: J Clin Microbiol. 1992 Jul;30(7):1654-60
references.pmid: 1629319
references.citation: J Dairy Sci. 1991 May;74(5):1544-9
references.pmid: 1880263
references.citation: Infect Immun. 2002 Feb;70(2):631-41
references.pmid: 11796592
references.citation: Vet Microbiol. 2005 May 20;107(3-4):295-9
references.pmid: 15863290
references.citation: Vet Microbiol. 2003 Oct 8;96(1):91-102
references.pmid: 14516711
references.citation: J Vet Sci. 2004 Jun;5(2):103-9
references.pmid: 15192336
references.citation: Mol Microbiol. 1995 Sep;17(6):1143-52
references.pmid: 8594333
references.citation: Nord Vet Med. 1974 Mar;26(3):197-204
references.pmid: 4598275
references.citation: Diagn Microbiol Infect Dis. 2004 Jul;49(3):157-62
references.pmid: 15246504
references.citation: Vet Microbiol. 2005 Mar 20;106(1-2):97-102
references.pmid: 15737478
references.citation: Clin Diagn Lab Immunol. 2003 Mar;10(2):272-7
references.pmid: 12626454
references.citation: Infect Immun. 2003 Apr;71(4):2292-5
references.pmid: 12654860
references.citation: J Dairy Sci. 2005 Nov;88(11):3810-7
references.pmid: 16230686
references.citation: J Appl Microbiol. 2007 Oct;103(4):993-1000
references.pmid: 17897203
references.citation: J Bacteriol. 2008 Oct;190(19):6302-17
references.pmid: 18567666
references.citation: Res Vet Sci. 2008 Dec;85(3):439-48
references.pmid: 18358507
references.citation: J Bacteriol. 2005 Jul;187(13):4584-91
references.pmid: 15968069
references.citation: J Appl Microbiol. 2003;95(1):38-43
references.pmid: 12807452
references.citation: J Clin Microbiol. 2003 Apr;41(4):1574-85
references.pmid: 12682148
references.citation: J Dairy Sci. 2006 Dec;89(12):4649-59
references.pmid: 17106097
references.citation: J Clin Microbiol. 1995 Sep;33(9):2233-9
references.pmid: 7494007