title: Synthesis, biophysical and repair studies of O6-2'-deoxyguanosine adducts by Escherichia coli OGT.
abstract: Oligonucleotides containing modified 2'-deoxyguanosines bearing a seven carbon linker at the O(6)- atom with either a terminal hydroxyl or 2'- deoxyguanosine group have been synthesized as potential intermediates formed during repair of interstrand cross-linked DNA. Repair of these substrates with Escherichia coli OGT was investigated with an assay involving cleavage of the unmodified duplex with the restriction endonuclease PvuII followed by analysis of the products by denaturing polyacrylamide gel electrophoresis. Duplexes containing these modifications were repaired by OGT suggesting that direct repair may play a role, in combination with other repair pathways, in reversing interstrand crosslink DNA damage.
authors.affiliation: Department of Chemistry & Biochemistry, Concordia University, 7141 Sherbooke St. West, Montreal, Canada.
authors.forename: Nadia M
authors.initials: NM
authors.lastname: Schoonhoven
authors.forename: Sebastian P
authors.initials: SP
authors.lastname: Murphy
authors.forename: Derek K
authors.initials: DK
authors.lastname: O'Flaherty
authors.forename: Anne M
authors.initials: AM
authors.lastname: Noronha
authors.forename: M Judith
authors.initials: MJ
authors.lastname: Kornblatt
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Wilds
mesh_terms: D018736:DNA Adducts / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D003849:Deoxyguanosine / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism*; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000378:metabolism*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism
chemical_list: D018736:DNA Adducts; D029968:Escherichia coli Proteins; D009841:Oligonucleotides; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase; C067511:Ogt protein, E coli; D003849:Deoxyguanosine