Conducting polymer-based impedimetric aptamer biosensor for in situ detection.
We have successfully developed the first prototype of an electrochemical protein biosensor using polypyrrole as the substrate and doped aptamer as the probe. The sensitivity for a specific target is 10 ng/ml. Two targets, platelet-derived growth factor and immunoglobulin E, have been tested. In both cases the sensor exhibited high specificity and the signal was found to increase as the target concentration increased.
Liao
Wei
W
Department of Bioengineering, University of Pittsburgh, 5063 Bioscience Tower 3, 3501 Fifth Ave, Pittsburgh, PA 15260, USA.
Randall
Brad A
BA
Alba
Nicolas A
NA
Cui
Xinyan Tracy
XT
D052157:Aptamers, Nucleotide / Q000032:analysis* / Q000737:chemistry; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry; D007073:Immunoglobulin E / Q000032:analysis*; D017403:In Situ Hybridization / Q000379:methods*; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D052157:Aptamers, Nucleotide; D010982:Platelet-Derived Growth Factor; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D007073:Immunoglobulin E