Modeling of trabecular bone and lamina dura following selective alveolar decortication in rats.
BACKGROUND
Modifying the balance between resorption and apposition through selectively injuring the cortical plate of the alveolus has been an approach to speed tooth movement and is referred to as periodontally accelerated osteogenic orthodontics. The aim of this study was to investigate the alveolar response to corticotomy as a function of time and proximity to the surgical injury in a rat model.


METHODS
Maxillary buccal and lingual cortical plates were injured in 36 healthy adult rats adjacent to the upper left first molars. Twenty-four animals were euthanized at 3, 7, or 11 weeks. In one group, the maxillae were removed and stripped of soft tissues, and histomorphometric analysis was performed to study alveolar spongiosa and periodontal ligament (PDL) modeling dynamics. Catabolic activity was analyzed with tartrate-resistant acid phosphatase-positive osteoclasts and preosteoclasts. Anabolic actions were measured using a fluorescent vital bone stain series followed by sacrifice at 30 and 51 days. To further analyze the new bone formation, a separate group of animals were fed with calcein fluorescent stain and processed for non-decalcified fluorescent stain histology.


RESULTS
At 3 weeks, the surgery group had significantly (P <0.05) less calcified spongiosa bone surface, greater periodontal ligament surface, higher osteoclast number, and greater lamina dura apposition width. The catabolic activity (osteoclast count) and anabolic activity (apposition rate) were three-fold greater, calcified spongiosa decreased by two-fold, and PDL surface increased by two-fold. Surgical injury to the alveolus that induced a significant increase in tissue turnover by week 3 dissipated to a steady state by postoperative week 11. The impact of the injury was localized to the area immediately adjacent to the decortication injury.


CONCLUSIONS
Selective alveolar decortication induced increased turnover of alveolar spongiosa, and the activity was localized; dramatic escalation of demineralization-remineralization dynamics is the likely biologic mechanism underlying rapid tooth movement following selective alveolar decortication.
Sebaoun
Jean-David
JD
Department of Orthodontics, School of Dental Medicine, Boston University, Boston, MA, USA.
Kantarci
Alpdogan
A
Turner
John W
JW
Carvalho
Roberto S
RS
Van Dyke
Thomas E
TE
Ferguson
Donald J
DJ
D000135:Acid Phosphatase / Q000032:analysis; D000539:Alveolar Process / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D000880:Anthraquinones; D015415:Biomarkers / Q000032:analysis; D016723:Bone Remodeling / Q000502:physiology; D001862:Bone Resorption / Q000378:metabolism / Q000503:physiopathology; D002452:Cell Count; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007527:Isoenzymes / Q000032:analysis; D008437:Maxilla / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D023421:Models, Animal; D010010:Osteoclasts / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology*; D010027:Osteotomy / Q000379:methods*; D010513:Periodontal Ligament / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D000071681:Tartrate-Resistant Acid Phosphatase; D013752:Tetracycline; D013997:Time Factors
D000880:Anthraquinones; D015415:Biomarkers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007527:Isoenzymes; C010078:alizarin; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D013752:Tetracycline; C007740:fluorexon
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