An in vivo multimodal imaging study using MRI and PET of stem cell transplantation after myocardial infarction in rats.
OBJECTIVE
The purpose of the study is to track iron-oxide nanoparticle-labelled adult rat bone marrow-derived stem cells (IO-rBMSCs) by magnetic resonance imaging (MRI) and determine their effect in host cardiac tissue using 2-deoxy-2-[F-18]fluoro-D: -glucose-positron emission tomography (FDG-PET).


METHODS
Infarcted rats were randomised to receive (1) live IO-rBMSCs by direct local injection, or (2) dead IO-rBMSCs as controls; (3) sham-operated rats received live IO-rBMSCs. The rats were then imaged from 2 days to 6 weeks post-cell implantation using both MRI at 9.4T and FDG-PET.


RESULTS
Implanted IO-rBMSCs were visible in the heart by MRI for the duration of the study. Histological analysis confirmed that the implanted IO-rBMSCs were present for up to 6 weeks post-implantation. At 1 week post-IO-rBMSC transplantation, PET studies demonstrated an increase in FDG uptake in infarcted regions implanted with live IO-rBMSC compared to controls.


CONCLUSIONS
Noninvasive multimodality imaging allowed us to visualise IO-rBMSCs and establish their affect on cardiac function in a rat model of myocardial infarction (MI).
Chapon
Catherine
C
MRC Clinical Sciences Centre, Imperial College London, UK.
Jackson
Johanna S
JS
Aboagye
Eric O
EO
Herlihy
Amy H
AH
Jones
William A
WA
Bhakoo
Kishore K
KK
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D005290:Ferric Compounds / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D019788:Fluorodeoxyglucose F18; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000000981:diagnostic imaging*; D053758:Nanoparticles; D010316:Particle Size; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*; D013997:Time Factors
D005290:Ferric Compounds; D005456:Fluorescent Dyes; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; C000499:ferric oxide; D016650:Fluorescein-5-isothiocyanate
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