Handling, storage, and preparation of human tissues.
Human tissue for flow cytometry must be prepared as an adequate single-cell suspension. The appropriate methods for tissue collection, transport, storage, and dissociation depend on the cell parameters being measured and the localization of the markers. This unit includes a general method for collecting and transporting human tissue and preparing a tissue imprint. Protocols are supplied for tissue disaggregation by either mechanical or enzymatic means and for preparation of single-cell suspensions of whole cells from fine-needle aspirates, pleural effusions, abdominal fluids, or other body fluids. Other protocols detail preparation of intact nuclei from fresh, frozen, or paraffin-embedded tissue. Support protocols cover fixation, cryospin preparation, cryopreservation, and removal of debris.
Dressler
L G
LG
University of North Carolina, Chapel Hill, North Carolina, USA.
Visscher
D
D
D001826:Body Fluids; D002467:Cell Nucleus / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D010232:Paraffin / Q000737:chemistry; D013048:Specimen Handling
D010232:Paraffin