title: Selective recognition of a tetra-amino-acid motif containing phosphorylated tyrosine residue by ribonucleopeptide.
abstract: We describe here a ribonucleopeptide (RNP) receptor targeting a tetra-amino-acid motif containing phosphotyrosine, GpYSR. GpYSR-binding RNP receptors were obtained from an RNA-based RNP library by in vitro selection. These receptors have a higher affinity than those of previously obtained pY-binding RNP receptors. One of these RNP receptors exhibited unique specificity to the target GpYSR peptide over other tetra-amino-acid peptides derived from the tyrosine-phosphorylation sites of native proteins. The GpYSR-binding RNP receptor discriminated not only the phosphorylated tyrosine residue, but also its surrounding three amino acid residues. Thus, RNP receptors could target a defined pY-containing amino-acid sequence by expanding the recognition surface within the ligand-binding pocket of RNP.
authors.affiliation: Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Nakano
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Masatora
authors.initials: M
authors.lastname: Fukuda
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Fujieda
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tainaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Morii
mesh_terms: D020816:Amino Acid Motifs; D010455:Peptides / Q000737:chemistry*; D019000:Phosphotyrosine / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
chemical_list: D010455:Peptides; D019000:Phosphotyrosine; D012313:RNA