title: Juxtamembrane basic residues in glycoprotein Ibbeta cytoplasmic domain are required for assembly and surface expression of glycoprotein Ib-IX complex.
abstract: Platelet glycoprotein (GP) Ib-IX complex requires all its three subunits for efficient expression on the cell surface, but the underlying molecular basis is not fully clear. Using transfected Chinese hamster ovary cells as the model system, we demonstrate that juxtamembrane residues 149-154 in the cytoplasmic domain of the GPIbbeta subunit is required for assembly and surface expression of the GPIb-IX complex. The complex, or GPIbbeta by itself, lacking these residues is retained in the endoplasmic reticulum. Our results thus have illustrated an important role of the GPIbbeta cytoplasmic domain in biosynthesis of the GPIb-IX complex.
authors.affiliation: Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Mo
authors.forename: Shi-Zhong
authors.initials: SZ
authors.lastname: Luo
authors.forename: José A
authors.initials: JA
authors.lastname: López
authors.forename: Renhao
authors.initials: R
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D003593:Cytoplasm / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000235:genetics; D014162:Transfection
chemical_list: D019038:Platelet Glycoprotein GPIb-IX Complex
references.citation: Nature. 1989 Oct 5;341(6241):456-8
references.pmid: 2677744
references.citation: Blood. 2004 Nov 15;104(10):3161-8
references.pmid: 15271795
references.citation: Blood. 1998 Jun 15;91(12):4397-418
references.pmid: 9616133
references.citation: Science. 2006 Dec 1;314(5804):1458-61
references.pmid: 17095657
references.citation: Circulation. 2005 Nov 8;112(19):2993-3000
references.pmid: 16260637
references.citation: Blood. 2002 Jun 15;99(12):4428-33
references.pmid: 12036872
references.citation: Blood. 1996 Feb 15;87(4):1377-84
references.pmid: 8608227
references.citation: J Biol Chem. 1992 Jun 25;267(18):12851-9
references.pmid: 1618785
references.citation: Exp Hematol. 2002 Apr;30(4):352-60
references.pmid: 11937271
references.citation: J Thromb Haemost. 2007 Dec;5(12):2494-502
references.pmid: 17922811
references.citation: Proc Natl Acad Sci U S A. 2007 May 22;104(21):9024-8
references.pmid: 17494758
references.citation: Blood. 1999 May 1;93(9):2968-75
references.pmid: 10216092
references.citation: Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16900-5
references.pmid: 17075060
references.citation: J Biol Chem. 1994 Sep 23;269(38):23716-21
references.pmid: 8089142
references.citation: Mol Cell Endocrinol. 2001 May 25;177(1-2):13-8
references.pmid: 11377815
references.citation: Blood. 2007 Jan 15;109(2):603-9
references.pmid: 17008541
references.citation: Am J Hematol. 2002 Dec;71(4):279-84
references.pmid: 12447957
references.citation: Curr Opin Cell Biol. 2002 Aug;14(4):417-22
references.pmid: 12383791
references.citation: J Biol Chem. 1998 Nov 20;273(47):31449-54
references.pmid: 9813057
references.citation: J Biol Chem. 2006 Aug 11;281(32):23050-9
references.pmid: 16757483
references.citation: Blood. 1995 Mar 1;85(5):1254-8
references.pmid: 7532036
references.citation: J Thromb Haemost. 2006 Jan;4(1):217-28
references.pmid: 16409472
references.citation: Biochemistry. 1997 Oct 14;36(41):12421-7
references.pmid: 9376345