title: Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences.
abstract: Circularizable oligonucleotide probes can detect short DNA sequences with single-base resolution at the site of ligation and can be amplified by rolling circle amplification (RCA) using strand displacing polymerases. A secondary amplification scheme was developed that uses the loop-mediated amplification reaction concurrent with RCA to achieve rapid signal development from the starting circular molecules. This isothermal reaction was found to be significantly faster than the comparable hyperbranching amplification method and could detect 100 circular copies in less than 1 h.
authors.affiliation: Department of Bioengineering, University of California-San Diego, La Jolla, CA, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Marciniak
authors.forename: Andrew
authors.initials: A
authors.lastname: Kummel
authors.forename: Sadik
authors.initials: S
authors.lastname: Esener
authors.forename: Michael
authors.initials: M
authors.lastname: Heller
authors.forename: Bradley
authors.initials: B
authors.lastname: Messmer
mesh_terms: D001483:Base Sequence; D011088:DNA Ligases / Q000378:metabolism; D015342:DNA Probes / Q000235:genetics; D004270:DNA, Circular / Q000737:chemistry / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics*; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D015342:DNA Probes; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D015345:Oligonucleotide Probes; D011088:DNA Ligases