Antibacterial effects of green tea polyphenols on clinical isolates of methicillin-resistant Staphylococcus aureus.
The antibacterial effects of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were evaluated. Characterization of the minimal inhibitory concentration (MIC) of oxacillin for 30 S. aureus strains isolated from patients treated with oxacillin identified 13 strains with an oxacillin MIC >or= 4 microg/mL as methicillin-resistant Staphylococcus aureus (MRSA) (range: 8 to 512 microg/mL), while 17 strains were methicillin-susceptible Staphylococcus aureus (MSSA) (range: 0.25-0.5 microg/mL). The MICs of TPP ranged from 50 to 180 microg/mL for both the MSSA and the MRSA strains. The MICs of oxacillin for each of the 13 MRSA strains were reduced between 8- and 128-fold when these strains were coincubated with sub-MIC (<or= 0.5x MIC) levels of TPP, demonstrating that the combination of TPP plus oxacillin was synergistic for all of the clinical MRSA isolates. Two-dimensional polyacrylamide gel electrophoresis identified 14 extracellular proteins of MRSA-13 down-regulated and 3 proteins up-regulated by exposure to TPP. These studies demonstrate that TPP can differentially stimulate the expression of various proteins in these bacteria and synergize the bactericidal activity of oxacillin for MRSA.
Cho
Yun-Seok
YS
Department of Biotechnology, Soonchunhyang University, P.O. Box 97, Asan, Chung-Nam 336-600, Republic of Korea.
Schiller
Neal L
NL
Oh
Kye-Heon
KH
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000032:analysis; D028241:Camellia sinensis / Q000737:chemistry*; D015169:Colony Count, Microbial; D015180:Electrophoresis, Gel, Two-Dimensional; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D010068:Oxacillin / Q000494:pharmacology / Q000627:therapeutic use; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols; D020543:Proteome / Q000032:analysis; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D020543:Proteome; D010068:Oxacillin
Biochim Biophys Acta. 1993 Apr 8;1147(1):132-6
8466924
Appl Environ Microbiol. 1995 Aug;61(8):2863-72
16535093
Proteomics. 2006 Feb;6(4):1301-18
16470664
Int J Antimicrob Agents. 2004 May;23(5):462-7
15120724
Electrophoresis. 1999 Dec;20(18):3551-67
10612281
Antimicrob Agents Chemother. 2002 Jul;46(7):2266-8
12069986
Curr Microbiol. 2002 Dec;45(6):415-22
12402082
Antimicrob Agents Chemother. 2004 Feb;48(2):546-55
14742208
Antimicrob Agents Chemother. 2001 Jun;45(6):1737-42
11353619
Curr Microbiol. 2004 Nov;49(5):346-52
15486709
Antimicrob Agents Chemother. 2005 Jul;49(7):2914-20
15980368
Proteomics. 2002 Jun;2(6):740-6
12112857
Antimicrob Agents Chemother. 2005 Oct;49(10):4339-43
16189116
Int J Antimicrob Agents. 2004 Oct;24(4):374-80
15380264
J Antimicrob Chemother. 2002 Dec;50(6):1051-4
12461032
J Antimicrob Chemother. 2001 Sep;48(3):361-4
11533000
Appl Microbiol Biotechnol. 2004 Aug;65(3):323-9
15309341
Curr Opin Infect Dis. 2006 Apr;19(2):161-8
16514341
Antimicrob Agents Chemother. 2002 Feb;46(2):558-60
11796378
Electrophoresis. 2001 May;22(9):1705-11
11425226
J Bacteriol. 1997 Dec;179(23):7595-9
9393730
Curr Microbiol. 2007 Dec;55(6):501-6
17874165
Antimicrob Agents Chemother. 2005 Feb;49(2):549-55
15673731