Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages.
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).
Beltran
S
S
UMR 5244 CNRS-EPHE, Parasitologie Fonctionnelle et Evolutive, Biologie et Ecologie Tropical et Méditerranéene, Université de Perpignan, Perpignan, France.
Galinier
R
R
Allienne
J F
JF
Boissier
J
J
D000818:Animals; D001700:Biomphalaria / Q000469:parasitology; D018509:DNA, Helminth / Q000302:isolation & purification*; D005838:Genotype; D008018:Life Cycle Stages; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000191:economics* / Q000379:methods; D015203:Reproducibility of Results; D012550:Schistosoma mansoni / Q000235:genetics*; D013997:Time Factors
D018509:DNA, Helminth