Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes.
Two proteins, YL41 and YL43, were isolated from 80S ribosomes of Saccharomyces cerevisiae by filtration through a Sephacryl S-200 column and by chromatography on a column of carboxymethylcellulose. Their amino acid compositions are presented. Twenty-four proteins including these two proteins were subjected to sequence analyses by automated Edman degradation. Amino-terminal amino acid sequences were determined for 17 proteins,YS3, YS9, YS23, YS24, YS29, YL6, YL8, YLll, YLI5,YL17, YL23, YL28, YL33, YL37, YL39, YL41, and YL43.YL41, which has a 72.7% lysine and arginine content, was found to be particular to eukaryotic ribosomes. The amino-termini of another seven proteins, YS2, YS5, YS8, YS12,YS13, YS20, and YS27, were suggested to be blocked. Comparison of the amino-terminal sequences with all other ribosomal protein sequences so far available indicates that YS9 shows sequence homology to rat liver ribosomal protein S8 (Wittmann-Liebold et al. 1979).
Otaka
E
E
Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University, 2-3, Kasumi 1 Chome, Minamiku, Hiroshima, 734 Japan.
Higo
K
K
Itoh
T
T
D000180:Acrylic Resins; D000595:Amino Acid Sequence; D000818:Animals; D002852:Chromatography, Ion Exchange; D008969:Molecular Sequence Data; D051381:Rats; D012269:Ribosomal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid
D000180:Acrylic Resins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C023049:sephacryl S 200
Biochem Biophys Res Commun. 1974 Oct 23;60(4):1482-90
4424298
Nucleic Acids Res. 1982 Oct 11;10(19):5869-78
6292856
Mol Gen Genet. 1983;191(3):519-24
6355773
Mol Gen Genet. 1982;185(2):239-44
6806564
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4403-7
6308621
Nucleic Acids Res. 1983 Nov 25;11(22):7759-68
6196722
Eur J Biochem. 1983 Nov 2;136(2):275-81
6414812
Annu Rev Biochem. 1979;48:719-54
382996
J Supramol Struct. 1979;12(4):425-33
398910
Nucleic Acids Res. 1983 May 25;11(10):3123-35
6304624
Eur J Biochem. 1979 Dec 17;102(2):573-82
393511
Biochemistry. 1979 Sep 18;18(19):4191-6
385049
Biochemistry. 1979 Dec 25;18(26):5787-91
391277
Biochem Biophys Res Commun. 1981 Feb 12;98(3):717-26
6784726
Biochemistry. 1982 Sep 14;21(19):4545-50
6814480