LacZ-based detection of acyl-homoserine lactone quorum-sensing signals.
Many bacteria use quorum-sensing regulatory systems to monitor their population density and to coordinate their genetic expression under certain conditions. Acyl-homoserine lactones are often produced by many Gram-negative bacteria and serve as quorum-sensing signal molecules. This unit describes two commonly used methods to detect acyl-homoserine lactones from laboratory or environmental bacterial cultures.
Joelsson
Adam C
AC
University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.
Zhu
Jun
J
D054742:Acyl-Butyrolactones / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003470:Culture Media; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007763:Lac Operon; D053038:Quorum Sensing; D015398:Signal Transduction; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D054742:Acyl-Butyrolactones; D003470:Culture Media; D001616:beta-Galactosidase