Cellular protection of morin against the oxidative stress induced by hydrogen peroxide.
Flavonoids are a class of secondary metabolites abundantly found in fruits and vegetables. In addition, flavonoids have been reported as potent antioxidants with beneficial effects against oxidative stress-related diseases such as cancer, aging, and diabetes. The present study was carried out to investigate the cytoprotective effects of morin (2',3,4',5,7-pentahydroxyflavone), a member of the flavonoid group, against hydrogen peroxide (H(2)O(2))-induced DNA and lipid damage. Morin was found to prevent the cellular DNA damage induced by H(2)O(2) treatment, which is shown by the inhibition of 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation (a modified form of DNA base), inhibition of comet tail (a form of DNA strand breakage), and decrease of nuclear phospho histone H2A.X expression (a marker for DNA strand breakage). In addition, morin inhibited membrane lipid peroxidation, which is detected by inhibition of thiobarbituric acid reactive substance (TBARS) formation. Morin was found to scavenge the intracellular reactive oxygen species (ROS) generated by H(2)O(2) treatment in cells, which is detected by a spectrofluorometer, flow cytometry, and confocal microscopy after staining of 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA). Morin also induces an increase in the activity of catalase and protein expression. The results of this study suggest that morin protects cells from H(2)O(2)-induced damage by inhibiting ROS generation and by inducing catalase activation.
Zhang
Rui
R
School of Medicine, Cheju National University, Jeju-si 690-756, Republic of Korea.
Kang
Kyoung Ah
KA
Piao
Mei Jing
MJ
Maeng
Young Hee
YH
Lee
Keun Hwa
KH
Chang
Weon Young
WY
You
Ho Jin
HJ
Kim
Ju Sun
JS
Kang
Sam Sik
SS
Hyun
Jin Won
JW
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D019610:Cytoprotection / Q000187:drug effects*; D004249:DNA Damage; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D047309:Flavones
D002374:Catalase; D005419:Flavonoids; D006861:Hydrogen Peroxide; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; C008548:morin; D047309:Flavones