title: Entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis.
abstract: Upon host infection, the protozoan parasite Entamoeba histolytica is confronted with reactive oxygen and nitrogen species and must survive these stresses in order to cause invasive disease. We analysed the parasite's response to oxidative and nitrosative stresses, probing the transcriptional changes of trophozoites of a pathogenic strain after a 60 min exposure to H2O2 (1 mM) or a NO donor (dipropylenetriamine-NONOate, 200 microM), using whole-genome DNA microarrays. Genes encoding reactive oxygen and nitrogen species detoxification enzymes had high transcriptional levels under basal conditions and upon exposure to both stresses. On a whole-genome level, there was significant modulation of gene expression by H2O2 (286 genes regulated) and dipropylenetriamine-NONOate (1036 genes regulated) with a significant overlap of genes modulated under both conditions (164 genes). A number of transcriptionally regulated genes were in signalling/regulatory and repair/metabolic pathways. However, the majority of genes with altered transcription encode unknown proteins, suggesting as yet unraveled response pathways in E. histolytica. Trophozoites of a non-pathogenic E. histolytica strain had a significantly muted transcriptional response to H2O2 compared with the pathogenic strain, hinting that differential response to oxidative stress may be one factor that contributes to the pathogenic potential of E. histolytica.
authors.affiliation: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5107, USA.
authors.forename: João B
authors.initials: JB
authors.lastname: Vicente
authors.forename: Gretchen M
authors.initials: GM
authors.lastname: Ehrenkaufer
authors.forename: Lígia M
authors.initials: LM
authors.lastname: Saraiva
authors.forename: Miguel
authors.initials: M
authors.lastname: Teixeira
authors.forename: Upinder
authors.initials: U
authors.lastname: Singh
mesh_terms: D000475:Alkenes / Q000494:pharmacology*; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D004748:Entamoeba histolytica / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017125:Genes, Protozoan; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015538:Nitrosation; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress
chemical_list: D000475:Alkenes; D000981:Antiprotozoal Agents; C453079:dipropylenetriamine-NONOate; D006861:Hydrogen Peroxide
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