MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin.
MicroRNAs (miRNAs) may function as oncogenes or tumor suppressors. Here, we show that miR-27a is up-regulated in human gastric adenocarcinoma. Suppression of miR-27a inhibits gastric cancer cell growth. Subsequently, prohibitin is identified as a potential miR-27a target, combining bioinformatics and microarray analysis. EGFP report experiment also confirms that the 3' untranslated region (3'UTR) of prohibitin carries the directly binding site of miR-27a. After knockdown of miR-27a in gastric cancer cells, mRNA level and protein level of prohibitin are both elevated. Down-regulation of prohibitin by miR-27a may explain why suppression of miR-27a can inhibit gastric cancer cell growth, further supporting that miR-27a functions as an oncogene.
Liu
Tao
T
Tianjin Life Science Research Center, Tianjin Medical University, No. 22, Qi-Xiang-Tai Road, Tianjin 300070, PR China.
Tang
Hua
H
Lang
Yuanyuan
Y
Liu
Min
M
Li
Xin
X
D020413:3' Untranslated Regions; D000230:Adenocarcinoma / Q000378:metabolism*; D001665:Binding Sites; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology*; D009857:Oncogenes; D000091402:Prohibitins; D012097:Repressor Proteins / Q000737:chemistry*; D013274:Stomach Neoplasms / Q000378:metabolism*
D020413:3' Untranslated Regions; C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D000091402:Prohibitins; D012097:Repressor Proteins; D049452:Green Fluorescent Proteins