title: Synthesis of N-4-butylamine acridone and its use as fluorescent probe for ctDNA.
abstract: A novel N-4-butylamine acridone (BAA), which is an acridone derivative was synthesized and characterized by IR, MS and (1)H NMR. The fluorescent characteristics of BAA was investigated in detail and used as the fluorescent probe for detection of calf thymus DNA (ctDNA). It was found that DNA was able to quench the fluorescence of BAA at 426 nm with the excitation at 254 nm. Under optimal conditions, the corresponding linear response range was from 1.0 to 20.0mg/L and the limit of detections (LOD) was 0.020 mg/L (defined as S/N=3). Moreover, the interaction between BAA and ctDNA was investigated by fluorescence, absorption and viscosity measurements. The results suggested that the interaction between BAA and ctDNA is groove binding in nature.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Fuzhou 350002, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Longhua
authors.initials: L
authors.lastname: Guo
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Chen
authors.forename: Yuwu
authors.initials: Y
authors.lastname: Chi
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D054831:Acridones / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D054831:Acridones; D015342:DNA Probes; D005456:Fluorescent Dyes; D004247:DNA; C427203:calf thymus DNA