[Kinetics of NDV-specific antibodies in chickens. II. Detection, importance and elimination of non-specific inhibitors and agglutinin in chicken sera].
The assay of Newcastle disease virus (NDV)-specific hemagglutinating inhibiting (HI) antibodies may be influenced by non-specific inhibitors (NSI) in chicken sera. The hemagglutination (HA) of various strains of influenza and parainfluenza Viruses is markedly reduced by alpha-NSI, present in chicken sera, whereas the strain Montana of NDV was shown to be resistant against these inhibitors. Therefore the latter strain can be employed for assays of NDV-specific antibodies in untreated sera. The detection of low Hl-titers in chicken sera Is important for answers of epizootological and biometrical questions. These low titers are easily detected by using an alpha-NSI resistant strain of NDV. The results of virus neutralisation- (VN-) tests can be influenced by beta-NSI, also present in chicken sera. The use of a beta-NSI resistant strain of NDV (e. g. Montana) results in specific titers at neutralisation indices of more than 1,0. This little neutralisation lis obviously caused by inhibitors which could not be 'inactivated or eliminated by treatment of sera with potassium meta-perjodate or kaolin.
Kaleta
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Institut fur Geflügelkrankheiten der Tierärztlichen Hochschule Hannover, Hannover, Bundesrepublik Deutschland.
Siegmann
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Lüders
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Janssen
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