Separation index: an easy-to-use metric for evaluation of different configurations on the same flow cytometer.
A quick and understandable method, using material readily available in a biology laboratory, for evaluating the measurement sensitivity of flow cytometers is presented in detail. Two examples of its use, in determining an optimal emission filter and in evaluating compensation, are also presented.
Bigos
Martin
M
Gladstone Institute of Virology and Immunology, San Francisco, California, USA.
D001695:Biology / Q000295:instrumentation / Q000379:methods; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity