Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells.
We examined a novel human feeder cell layer of mesenchymal stem cells harvested from human adipose tissues. Gene expression analyses and colony-forming assay with human primary epithelial cells showed that the adipose tissue-derived mesenchymal stem cells produced various factors to support epithelial stem/progenitor maintenance and cell growth. Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces.
Sugiyama
Hiroaki
H
Department of Ophthalmology, Osaka University Medical School, Osaka 565-0871, Japan.
Maeda
Kazuhisa
K
Yamato
Masayuki
M
Hayashi
Ryuhei
R
Soma
Takeshi
T
Hayashida
Yasutaka
Y
Yang
Joseph
J
Shirakabe
Masayuki
M
Matsuyama
Akifumi
A
Kikuchi
Akihiko
A
Sawa
Yoshiki
Y
Okano
Teruo
T
Tano
Yasuo
Y
Nishida
Kohji
K
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008858:Microscopy, Phase-Contrast / Q000379:methods; D008954:Models, Biological; D041681:NIH 3T3 Cells; D013234:Stem Cells; D023822:Tissue Engineering / Q000379:methods