title: Novozyme 435-catalyzed efficient acylation of 3-n-butylphthalide in organic medium.
abstract: Novozyme 435 could catalyze efficient acylation of 3-n-butylphthalide in organic medium. The conversion of 3-n-butylphthalide increased with the increase of hydrophobicity of solvent below that of hexane. The more available solvent was hexane. Salt hydride could control fixed water activity. The optimum water activity was 0.62. And the optimum of reaction time, velocity of agitation, dosage of Novozyme 435 and acetic anhydride to 3-n-butylphtrhalide molar ratio were 48 hours, 150 rpm, 8 mg/mL and 8:1, respectively. The conversion of 48.9% could be obtained at a water activity of 0.62 in hexane. Furthermore, Novozyme 435 had an enantioselective acylation of racemic 3-n-butylphthalide by original analysis.
authors.affiliation: Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province, P R China.
authors.forename: Laping
authors.initials: L
authors.lastname: He
authors.forename: Jiong
authors.initials: J
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Sun
authors.forename: Changge
authors.initials: C
authors.lastname: Zheng
mesh_terms: D000215:Acylation; D001572:Benzofurans / Q000737:chemistry*; D002384:Catalysis; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry*
chemical_list: D001572:Benzofurans; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D009930:Organic Chemicals; C027125:3-n-butylphthalide; C093667:Novozyme 435; D008049:Lipase