Proper alignment and adjustment of the light microscope.
The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications (e.g., brightfield, phase-contrast, differential interference contrast, and fluorescence epi-illumination), and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The principles of microscopy and step-by-step alignment and adjustment procedures are described in this unit.
Salmon
Edward D
ED
University of North Carolina, Chapel Hill, North Carolina, USA.
von Lackum
Kate
K
Canman
Julie C
JC
D019411:Clinical Laboratory Techniques; D008027:Light; D008853:Microscopy / Q000295:instrumentation* / Q000592:standards*