title: Total glutathione and glutathione reductase in bovine erythrocytes and liver biopsy.
abstract: The goal of the present study was to measure the total glutathione level and glutathione reductase activity in bovine erythrocytes and liver biopsy. Five cows were injected intraperitoneally with DL-ethionine (12.5 mg/kg B.W.), and two control cows were injected with normal saline (0.9% NaCl). Ultrasonography guided liver biopsy, and blood samples were collected at 0, 4, 7 and 10 days after injection. The hepatic total glutathione level was significantly increased on Days 7 (p<0.05) and 10 (p<0.01), and hepatic glutathione reductase activity was significantly increased on Days 4 (p<0.05), 7 (p<0.01) and 10 (p<0.01). There were insignificant changes in the erythrocytic total glutathione level. The present study demonstrated that liver biopsy is a valuable tool for detecting oxidative stress and for diagnosing hepatic dysfunction in cattle from the viewpoint of the status of glutathione and glutathione reductase.
authors.affiliation: Veterinary Teaching Hospital, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: Mahmoud Rushdi
authors.initials: MR
authors.lastname: Abd Ellah
authors.forename: Keiji
authors.initials: K
authors.lastname: Okada
authors.forename: Masanobu
authors.initials: M
authors.lastname: Goryo
authors.forename: Saori
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Oishi
authors.forename: Jun
authors.initials: J
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D001706:Biopsy; D002417:Cattle; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005001:Ethionine / Q000494:pharmacology; D005978:Glutathione / Q000097:blood / Q000378:metabolism*; D005980:Glutathione Reductase / Q000097:blood / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008112:Liver Glycogen / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D008112:Liver Glycogen; D005980:Glutathione Reductase; D005978:Glutathione; D005001:Ethionine