title: Enhancement of alkaline polygalacturonate lyase production in recombinant Pichia pastoris according to the ratio of methanol to cell concentration.
abstract: Polygalacturonate lyase (PGL) production by Pichia pastoris GS115 was used as a model to study the mechanism and strategy for enhancing heterologous protein production. It was found that the ratio of methanol to cell concentration had a significant influence on PGL production. In this study, an advanced glycerol exponential feeding strategy was developed for biomass accumulation in cell growth phase, by which cell concentration reached 140 g L(-1) after 19 h glycerol feeding. In subsequent production phase, a methanol feeding profile was proposed according to the optimal ratio of methanol to cell concentration at a range of 0.163-0.171 g g(-1), and PGL activity and productivity reached 430 U mL(-1) and 4.34 U mL(-1)h(-1), respectively. The strategy for enhancing PGL production by controlling the optimal ratio may provide an alternative approach to enhance heterologous protein production with P. pastoris.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Wang
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Zhaozhe
authors.initials: Z
authors.lastname: Hua
authors.forename: Liming
authors.initials: L
authors.lastname: Liu
authors.forename: Jianghua
authors.initials: J
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D000468:Alkalies / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods*; D000432:Methanol / Q000378:metabolism*; D010843:Pichia / Q000201:enzymology* / Q000235:genetics; D011133:Polysaccharide-Lyases / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D000468:Alkalies; D011994:Recombinant Proteins; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D000432:Methanol