Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia.
We evaluated the Plasmodium vivax polymorphism by studying the Pvmsp-3alpha gene's polymorphic region by PCR-RFLP in 55 samples from patients living in Tierralta, Colombia. Three different sizes of the Pvmsp-3 alpha gene were found, type A (1,900 bp), type B (1,500 bp) and type C (1,100 bp); most of the samples were type A (96.4 %). The Pvmsp-3alpha gene exhibited high polymorphism. Seven restriction patterns were found when using Alu I, and nine were found with Hha I; 12 different alleles were obtained when these patterns were combined. The findings suggest that this gene could be used in Colombia as a molecular epidemiologic marker for genotyping P. vivax.
Cristiano
Fabio Aníbal
FA
Grupo de Bioquímica y Biología Celular, Instituto Nacional de Salud, Bogotá, DC, Colombia.
Pérez
Manuel Alberto
MA
Nicholls
Rubén Santiago
RS
Guerra
Angela Patricia
AP
D000818:Animals; D003105:Colombia; D016054:DNA, Protozoan / Q000235:genetics*; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D010966:Plasmodium vivax / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D016054:DNA, Protozoan; D005819:Genetic Markers