[Establishment of a mouse model of biliary obstruction and its dynamic observations].
OBJECTIVE
To establish a mouse model of biliary obstruction.


METHODS
Sixty-four Balb/c mice were divided into experimental group and control group. Obstructive jaundice was induced in the mice in the experimental group by common bile duct ligation. The level of the common bile duct diameter, WBC, LYM MID, LYM%, MID% and ALT, AST, TBIL, DBIL, IBIL, ALP and CHOL were measured 12 h and 1, 2 ,3, 4, 5, and 7 days after the ligation. The morphological changes in the liver were also observed.


RESULTS
The level of common bile duct diameter, WBC, LYM, MID, LYM%, MID% and ALT, AST, TBIL, DBIL, ALP and CHOL all underwent changes with time following certain patterns.


CONCLUSIONS
The jaundice manifestation of this model is similar to that of patients with biliary obstruction, and this model may provide a reliable model for studying the mechanism of obstructive jaundice.
Zhang
Shou-hua
SH
Department of Hepatobiliary Surgery, Nanfang Hospital.
Liao
Cai-xian
CX
Zhang
Chun-xing
CX
Deng
Hong
H
Zhu
Hang
H
Lei
Lei
L
Yao
Chen
C
D000818:Animals; D001651:Cholestasis, Extrahepatic / Q000473:pathology*; D003135:Common Bile Duct / Q000473:pathology* / Q000601:surgery; D004195:Disease Models, Animal; D005260:Female; D008026:Ligation; D008099:Liver / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C