[Matrine inhibits the proliferation of prostate cancer cells and the activity of androgen receptor].
OBJECTIVE
To study the inhibitory effect of matrine on the proliferation of the prostate cancer cell line LNCaP and the expression of the androgen receptor (AR).


METHODS
LNCaP cells were treated with matrine at the concentration of 0.5, 1.0, 1.5, 2.0 and 3.0 g/L for 12, 24 and 36 hours, the cell growth activity determined by MTT colorimetry and trypan blue staining at 36 hours, the cell cycle changes detected by flow cytometry and the expression of AR by Western blot at 24 hours.


RESULTS
Matrine suppressed the in vitro growth of the androgen-sensitive prostate cancer cell line LNCaP in a time- and dose-dependent manner, blocked the cell cycles in the G2/M phase and decreased the expression of AR in the cell line in a dose-dependent manner (P < 0.01).


CONCLUSIONS
Matrine can significantly inhibit the in vitro growth of NCaP cells by down-regulating the expression of AR and blocking cell cycles.
Chen
Ke
K
Department of Urology, Tongji Hospital, Wuhan, Hubei 430030, China.
Hu
Zhi-Quan
ZQ
Wang
Tao
T
Guo
Jun-Li
JL
Guo
Hui
H
Ye
Zhang-Qun
ZQ
D000470:Alkaloids / Q000494:pharmacology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D011807:Quinolizines / Q000494:pharmacology*; D011944:Receptors, Androgen / Q000378:metabolism*; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; D011944:Receptors, Androgen; D000093842:Matrines