MSX-3D: a tool to validate 3D protein models using mass spectrometry.
BACKGROUND
The technique of chemical cross-linking followed by mass spectrometry has proven to bring valuable information about the protein structure and interactions between proteic subunits. It is an effective and efficient way to experimentally investigate some aspects of a protein structure when NMR and X-ray crystallography data are lacking.


RESULTS
We introduce MSX-3D, a tool specifically geared to validate protein models using mass spectrometry. In addition to classical peptides identifications, it allows an interactive 3D visualization of the distance constraints derived from a cross-linking experiment.


BACKGROUND
Freely available at http://proteomics-pbil.ibcp.fr
Heymann
Michaël
M
Institut de Biologie et Chimie des Protéines (IBCP) UMR5086 CNRS, Université Lyon 1, France.
Paramelle
David
D
Subra
Gilles
G
Forest
Eric
E
Martinez
Jean
J
Geourjon
Christophe
C
Deléage
Gilbert
G
D003198:Computer Simulation; D030562:Databases, Protein; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D012984:Software
D011506:Proteins