title: Nuclear matrix contains novel WD-repeat and disordered-region-rich proteins.
abstract: To find novel proteins predicted to participate in the formation of nuclear bodies, nuclear speckles, and nuclear macro-protein complexes, we applied proteome analysis to a HeLa cell nuclear matrix fraction. Proteins in the fraction were separated by SDS-PAGE, digested with trypsin, and analyzed by nanoflow liquid chromatography-iontrap-tandem mass spectrometry. Three hundred and thirty three proteins including 39 novel ones were identified. Seven WD-repeat proteins and 16 disordered region-rich proteins, which act frequently as scaffolding proteins for macro-protein complexes, were found amongst the novel proteins.
authors.affiliation: Graduate School of Science and Technology, Niigata University, Igarashi-2, Nishi-ku, Niigata 950-2181, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Ishii
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Hirano
authors.forename: Nanase
authors.initials: N
authors.lastname: Araki
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Oda
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kumeta
authors.forename: Kunio
authors.initials: K
authors.lastname: Takeyasu
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Furukawa
authors.forename: Tsuneyoshi
authors.initials: T
authors.lastname: Horigome
mesh_terms: D006367:HeLa Cells; D006801:Humans; D015530:Nuclear Matrix / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D020543:Proteome; D020449:Repetitive Sequences, Amino Acid
chemical_list: D009687:Nuclear Proteins; D020543:Proteome