title: Genetic diversity among isolates of Paenibacillus larvae from Austria.
abstract: Genetic diversity of 214 Paenibacillus larvae strains from Austria was studied. Genotyping of isolates was performed by polymerase chain reaction (PCR) with primers corresponding to enterobacterial repetitive intergenic consensus (ERIC), BOX repetitive and extragenic palindromic (REP) elements (collectively known as rep-PCR) using ERIC primers, BOX A1R and MBO REP1 primers. Using ERIC-PCR technique two genotypes could be differentiated (ERIC I and II), whereas using combined typing by BOX- and REP-PCR, five different genotypes were detected (ab, aB, Ab, AB and alphab). Genotypes aB and alphab are new and have not been reported in other studies using the same techniques.
authors.affiliation: Institute for Apiculture, Austrian Agency for Health and Food Safety (AGES), Vienna, Austria.
authors.forename: Igor
authors.initials: I
authors.lastname: Loncaric
authors.forename: Irmgard
authors.initials: I
authors.lastname: Derakhshifar
authors.forename: Josua T
authors.initials: JT
authors.lastname: Oberlerchner
authors.forename: Hemma
authors.initials: H
authors.lastname: Köglberger
authors.forename: Rudolf
authors.initials: R
authors.lastname: Moosbeckhofer
mesh_terms: D001317:Austria; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D005838:Genotype; D016133:Polymerase Chain Reaction