title: Genetic characterization of Staphylococcus aureus isolates carrying Panton-Valentine leukocidin genes in Bangladesh.
abstract: To assess the spread and genetic characteristics of Panton-Valentine leukocidin (PVL) gene-carrying Staphylococcus aureus in Bangladesh, we investigated 59 strains (49 isolates from clinical specimens and 10 isolates colonized in the nasal cavities of medical staff), including 26 methicillin-resistant S. aureus (MRSA) strains. The PVL gene was detected only in methicillin-susceptible S. aureus (MSSA) strains (7 clinical strains and 2 colonizing strains). PVL gene-positive MSSA strains were found to belong to coagulase serotypes III or VI and were classified into sequence types ST88 (CC88), ST772, and ST573 (CC1) by multilocus sequence typing, and agr types 2 or 3. These types were different from those determined for MRSA (coagulase serotypes I and IV, ST240 and ST361, and agr type 1). PVL gene-positive MSSA possessed a larger number of virulence factor genes than MRSA, although they were susceptible to more antimicrobials. These findings suggest that the PVL gene is distributed to limited populations of S. aureus clones with specific genetic traits that are distinct from MRSA in Bangladesh, but genetically close to CA-MRSA clones in the CC1 lineage reported in the United States and European countries.
authors.affiliation: Department of Microbiology, Sir Salimullah Medical College, Dhaka, Bangladesh.
authors.forename: Sadia
authors.initials: S
authors.lastname: Afroz
authors.forename: Nobumichi
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nagashima
authors.forename: M Mahbub
authors.initials: MM
authors.lastname: Alam
authors.forename: A B M Bayezid
authors.initials: AB
authors.lastname: Hossain
authors.forename: M Abdur
authors.initials: MA
authors.lastname: Rahman
authors.forename: M Rafiqul
authors.initials: MR
authors.lastname: Islam
authors.forename: Afzalunnessa Binte
authors.initials: AB
authors.lastname: Lutfor
authors.forename: Naima
authors.initials: N
authors.lastname: Muazzam
authors.forename: M Abul Hossain
authors.initials: MA
authors.lastname: Khan
authors.forename: Shyamal Kumar
authors.initials: SK
authors.lastname: Paul
authors.forename: A K M
authors.initials: AK
authors.lastname: Shamsuzzaman
authors.forename: M Chan
authors.initials: MC
authors.lastname: Mahmud
authors.forename: A K M
authors.initials: AK
authors.lastname: Musa
authors.forename: M Akram
authors.initials: MA
authors.lastname: Hossain
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics* / Q000302:isolation & purification; D001459:Bangladesh / Q000453:epidemiology; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D003030:Coagulase / Q000378:metabolism; D005098:Exotoxins / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007956:Leukocidins / Q000235:genetics* / Q000302:isolation & purification; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013207:Staphylococcal Skin Infections / Q000453:epidemiology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003030:Coagulase; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D008712:Methicillin