Potent specific immune responses induced by prime-boost-boost strategies based on DNA, adenovirus, and Sendai virus vectors expressing gag gene of Chinese HIV-1 subtype B.
To study the immune responses elicited by multiple vectors and develop vaccines strategies against prevalent HIV-1 strains in China, we have examined the potency of vaccine regimens of plasmid DNA, adenovirus, and Sendai virus vectors expressing HIV-1 gag consensus sequence of HIV-1 isolates from China for inducing specific immune responses. In BALB/c mice, combination of these vectors induced higher Gag-specific cellular immune response than any regimen using single vector alone. The prime-boost-boost regimen consisting of the triple heterologous vectors induced Gag-specific T-cell responses the most efficiently. In rhesus macaques, the prime-boost-boost regimen induced potent Gag-specific cellular immune responses as well as long lasting humoral immune response, and each booster resulted in rapid and efficient expansion of Gag-specific T cells. These results indicate that this prime-boost-boost regimen using triple heterologous vectors is a promising AIDS vaccine candidate for efficiently inducing HIV-1-specific cellular and humoral immune responses. Its further studies as a promising scheme for therapeutic and/or prophylactic HIV-1 vaccines should be grounded.
Yu
Shuangqing
S
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
Feng
Xia
X
Shu
Tsugumine
T
Matano
Tetsuro
T
Hasegawa
Mamoru
M
Wang
Xiaoli
X
Ma
Hongtao
H
Li
Hongxia
H
Li
Zelin
Z
Zeng
Yi
Y
D016915:AIDS Vaccines / Q000235:genetics* / Q000276:immunology*; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015683:Gene Products, gag / Q000096:biosynthesis; D015750:Genes, gag / Q000235:genetics* / Q000276:immunology*; D005822:Genetic Vectors / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D029082:Sendai virus / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology
D016915:AIDS Vaccines; D015683:Gene Products, gag; D019444:Vaccines, DNA; D007371:Interferon-gamma