title: Higher levels of salivary MUC5B and MUC7 in individuals with gastric diseases who harbor Helicobacter pylori.
abstract: OBJECTIVE
  The aim of the present study was to assess the salivary levels of MUC5B and MUC7 in individuals with dyspeptic disease and Helicobacter pylori (H. pylori) in the stomach, compared to individuals without dyspeptic disease.
  
  
  METHODS
  30 individuals with dyspeptic disease, who underwent endoscopy for upper gastrointestinal complaints at Hospital Pedro Ernesto-RJ, Brasil and tested positive for H. pylori, and 23 controls with no dyspeptic disease, with mean age 53.5+/-4.4 years, were included in the study. Saliva samples and 3 antral biopsy were taken for PCR analysis and histologic examination. In addition, saliva samples were tested by ELISA with F2 monoclonal antibody and EU7A antibody against MUC7, to determine MUC5B and MUC7 levels, prior to endoscopic examination. The expression pattern of the proteins was quantified by comparison to a pooled saliva sample of 19 healthy volunteers.
  
  
  RESULTS
  MUC5B and MUC7 salivary levels were higher in the individuals with dyspeptic disease than in controls (p<0.0001). 33.3% (9/30) of the dyspeptic individuals and 0% of the controls had H. pylori in the oral cavity.
  
  
  CONCLUSIONS
  Individuals with gastric diseases, with H. pylori in the stomach, showed higher levels of salivary H. pylori receptors-MUC5B and MUC7-than individuals without gastric diseases. These results suggest that higher levels of specific salivary mucins could be useful as risk indicators for infection by H. pylori.
authors.affiliation: Escola de Odontologia, UNIGRANRIO, Duque de Caxias, Brazil. denisegomes@globo.com
authors.forename: Denise G
authors.initials: DG
authors.lastname: Silva
authors.forename: Roy H
authors.initials: RH
authors.lastname: Stevens
authors.forename: Jacyara M B
authors.initials: JM
authors.lastname: Macedo
authors.forename: Raphael
authors.initials: R
authors.lastname: Hirata
authors.forename: Amanda C
authors.initials: AC
authors.lastname: Pinto
authors.forename: Luciano M
authors.initials: LM
authors.lastname: Alves
authors.forename: Enno C I
authors.initials: EC
authors.lastname: Veerman
authors.forename: Eduardo M B
authors.initials: EM
authors.lastname: Tinoco
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001938:Brazil; D016022:Case-Control Studies; D015169:Colony Count, Microbial; D004415:Dyspepsia / Q000378:metabolism / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D055257:Mucin-5B / Q000032:analysis*; D009077:Mucins / Q000032:analysis*; D012463:Saliva / Q000737:chemistry* / Q000382:microbiology; D012471:Salivary Proteins and Peptides / Q000032:analysis*
chemical_list: D015415:Biomarkers; C524578:MUC5B protein, human; C083238:MUC7 protein, human; D055257:Mucin-5B; D009077:Mucins; D012471:Salivary Proteins and Peptides