title: Survival rate of preantral follicles derived from vitrified neonate mouse ovarian tissue by Cryotop and conventional methods.
abstract: The aim of this study was to investigate the growth and survival rate of preantral follicles isolated from vitrified ovarian tissue by Cryotop and conventional methods. The ovaries of 14-day-old mice were separated and divided into four groups as following: Cryotop group, vitrified by Cryotop; CV (Conventional; CV) group, vitrified by conventional straw; toxicity test group and control group. After warming the vitrified ovaries, isolated preantral follicles from four groups were cultured for 4 days to compare survival rate and follicular growth between above-mentioned groups. Survival rate (97.3%) in toxicity test group alike the control group (98.7%) were significantly higher (P<0.05) than the Cryotop (92.7%) and CV (47.7%) groups. Increase in follicle diameters after 4 days in Cryotop and CV groups was significantly lower (P<0.05) than the control and toxicity test groups, but growth and survival rate of follicles in Cryotop group was significantly higher (P<0.05) than the CV group. These results demonstrated that ovarian tissue vitrification by Cryotop highly preserves the viability rate of preantral follicles.
authors.affiliation: Department of Embryology, Reproductive Medicine Center, Royan Institute, Tehran, Iran. Eimanih@yahoo.com
authors.forename: H
authors.initials: H
authors.lastname: Eimani
authors.forename: S S
authors.initials: SS
authors.lastname: Mamzoji
authors.forename: M M
authors.initials: MM
authors.lastname: Soleimani
authors.forename: M H
authors.initials: MH
authors.lastname: Abnosi
authors.forename: M Rezazadeh
authors.initials: MR
authors.lastname: Valojerdi
authors.forename: P Eftekhari
authors.initials: PE
authors.lastname: Yazdi
authors.forename: A
authors.initials: A
authors.lastname: Shahverdi
authors.forename: H
authors.initials: H
authors.lastname: Guorabi
mesh_terms: D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D051379:Mice; D009924:Organ Culture Techniques / Q000379:methods; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development*