Berberine inhibits SDF-1-induced AML cells and leukemic stem cells migration via regulation of SDF-1 level in bone marrow stromal cells.
Berberine plays a prominent role on the control of tumor cell invasion and migration. SDF-1 is a homeostatic chemokine that signals through CXCR4 which is expressed by hematopoietic tumor cells. The SDF-1/CXCR4 axis is involved in the migration process of leukemic cells. In this study, we investigated the effects of berberine on the SDF-1-induced HL-60 cells, primary acute myeloid leukemia (AML) cells and leukemic stem cells (LSCs) migration. Transwell migration chambers (8 microm) were used to assess the role of berberine on leukemic cell migration; Flow cytometry was used to analyze the role of berberine on the CXCR4 expression; SDF-1 protein level secreted by bone marrow stromal cells (BMSCs) was evaluated by ELISA. Results demonstrated that berberine could partly inhibit SDF-1-induced AML cells as well as LSCs migration. Berberine could reduce SDF-1 protein level secreted by BMSCs in the microenvironment but not affect CXCR4 expression on HL-60 cell membrane, and we hypothesized that berberine could inhibit AML cells migration partly by reducing the secreting of SDF-1 by BMSCs and inhibiting HERG1 K(+) channels of leukemic cells. Therefore, it is speculated that berberine might be a potentially effective agent for prevention of leukemia.
Li
Huiyu
H
Center for Stem Cell Research and Application, Union Hospital of Huazhong University of Science and Technology, 1277# Jiefang Avenue, Wuhan, Hubei 430022, PR China.
Guo
Linlin
L
Jie
Shenghua
S
Liu
Wei
W
Zhu
Jiang
J
Du
Wen
W
Fan
Lingling
L
Wang
Xiaojuan
X
Fu
Bingjie
B
Huang
Shiang
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001599:Berberine / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000502:physiology; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000378:metabolism*; D002648:Child; D003864:Depression, Chemical; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000502:physiology
D016588:Anticarcinogenic Agents; D054377:Chemokine CXCL12; D001599:Berberine