title: In vitro and in vivo validation of ligA and tarI as essential targets in Staphylococcus aureus.
abstract: A conditional expression system has been developed using the isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible Pspac promoter to validate essential genes of Staphylococcus aureus in vivo. The system has been applied to prove the essentiality of ligA and to evaluate the function of tarI, which was found to be essential in vitro but not in vivo.
authors.affiliation: Universität Würzburg, Institut für Molekulare Infektionsbiologie, Röntgenring 11, 97070 Würzburg, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Streker
authors.forename: Tina
authors.initials: T
authors.lastname: Schäfer
authors.forename: Christoph
authors.initials: C
authors.lastname: Freiberg
authors.forename: Heike
authors.initials: H
authors.lastname: Brötz-Oesterhelt
authors.forename: Jörg
authors.initials: J
authors.lastname: Hacker
authors.forename: Harald
authors.initials: H
authors.lastname: Labischinski
authors.forename: Knut
authors.initials: K
authors.lastname: Ohlsen
mesh_terms: D000038:Abscess / Q000382:microbiology; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D003470:Culture Media; D011088:DNA Ligases / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D020043:Genes, Essential; D006801:Humans; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D051379:Mice; D009154:Mutation; D013207:Staphylococcal Skin Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D007544:Isopropyl Thiogalactoside; D011088:DNA Ligases