Genotoxic effect of Physalis angulata L. (Solanaceae) extract on human lymphocytes treated in vitro.
Physalis angulata L (Solanaceae) is a medicinal plant from North of Brazil, whose different extracts and infusions are commonly used in the popular medicine for the treatment of malaria, asthma, hepatitis, dermatitis and rheumatism. However, the genotoxic effects of P. angulata on human cells is not well known. The main purpose of the present study was to evaluate the in vitro genotoxic effects of aqueous extract of P. angulata using the comet assay and the micronucleus assay in human lymphocytes provided from 6 healthy donors. Treatments with P. angulata extracts were performed in vitro in order to access the extent of DNA damage. The comet assay has shown that treatments with P. angulata at 0.5, 1.0, 2.0, 3.0 and 6.0 microg/mL in culture medium were genotoxic. Lymphocytes treated with P. angulata at the concentrations of 3.0 and 6.0 microg/mL in culture medium showed a statistically significant increase in the frequency of micronucleus (p<0.05), however, the cytokinesis blocked proliferation index (CBPI) was not decreased after P. angulata treatment. In conclusion, the present work demonstrated the genotoxic effects of P. angulata extract on human lymphocytes in vitro.
Alves dos Santos
Raquel
R
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Cabral
Teresinha Rosa
TR
Cabral
Isabel Rosa
IR
Antunes
Lusânia Maria
LM
Pontes Andrade
Cristiane
C
Cerqueira dos Santos Cardoso
Plínio
P
de Oliveira Bahia
Marcelo
M
Pessoa
Claudia
C
Martins do Nascimento
José Luis
JL
Rodríguez Burbano
Rommel
R
Takahashi
Catarina Satie
CS
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D020552:Comet Assay; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D009153:Mutagens / Q000494:pharmacology*; D032321:Physalis / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity*
D009153:Mutagens; D010936:Plant Extracts