GermSAGE: a comprehensive SAGE database for transcript discovery on male germ cell development.
GermSAGE is a comprehensive web-based database generated by Serial Analysis of Gene Expression (SAGE) representing major stages in mouse male germ cell development, with 150,000 sequence tags in each SAGE library. A total of 452,095 tags derived from type A spermatogonia (Spga), pachytene spermatocytes (Spcy) and round spermatids (Sptd) were included. GermSAGE provides web-based tools for browsing, comparing and searching male germ cell transcriptome data at different stages with customizable searching parameters. The data can be visualized in a tabulated format or further analyzed by aligning with various annotations available in the UCSC genome browser. This flexible platform will be useful for gaining better understanding of the genetic networks that regulate spermatogonial cell renewal and differentiation, and will allow novel gene discovery. GermSAGE is freely available at http://germsage.nichd.nih.gov/
Lee
Tin-Lap
TL
Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Cheung
Hoi-Hung
HH
Claus
Janek
J
Sastry
Chandan
C
Singh
Sumeeta
S
Vu
Loc
L
Rennert
Owen
O
Chan
Wai-Yee
WY
D000818:Animals; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D023281:Genomics; D008297:Male; D051379:Mice; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism*