Lavandulyl flavanones from Sophora flavescens protect mouse hippocampal cells against glutamate-induced neurotoxicity via the induction of heme oxygenase-1.
Lavandulyl flavanones of Sophora flavescens roots are anti-malarial, anti-inflammatory, and cytotoxic. Here, we examined whether four lavandulyl flavanones, (2S)-2'-methoxykurarinone (1), sophoraflavanone G (2), leachianone A (3), and (-)-kurarinone (4), isolated from S. flavescens could protect HT22 immortalized hippocampal cells against glutamate-induced oxidative stress. Compounds 1 and 2 induced the expression of heme oxygenase (HO)-1 and increased HO activity dose- and time-dependently. These two compounds also suppressed glutamate-induced reactive oxygen species generation in HT22 cells, whereas compounds 3 and 4 were not protective. These two lavandulyl flavanones (compounds 1, 2) may protect against glutamate-induced neurotoxicity via HO-1 induction.
Jeong
Gil-Saeng
GS
Institute for Radiological Imaging Science, Wonkwang University, Iksan 570-749, Korea.
Li
Bin
B
Lee
Dong-Sung
DS
Byun
Erisa
E
An
Ren-Bo
RB
Pae
Hyun-Ock
HO
Chung
Hun-Taeg
HT
Youn
Kwon-Ha
KH
Kim
Youn-Chul
YC
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity*; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology*; D018698:Glutamic Acid / Q000633:toxicity*; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D029909:Sophora / Q000737:chemistry*; D015854:Up-Regulation / Q000187:drug effects
D018690:Excitatory Amino Acid Agonists; D044950:Flavanones; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D018698:Glutamic Acid; D051547:Heme Oxygenase-1