title: Transcriptomic responses of European flounder (Platichthys flesus) to model toxicants.
abstract: The temporal transcriptomic responses in liver of Platichthys flesus to model environmental pollutants were studied over a 16-day time span after intraperitoneal injection with cadmium chloride (50 microg/kg in saline), 3-methylcholanthrene (25 mg/kg in olive oil), Aroclor 1254 (50 mg/kg in olive oil), tert-butyl-hydroperoxide (5 mg/kg in saline), Lindane (25mg/kg in olive oil), perfluoro-octanoic acid (100 mg/kg in olive oil) and their vehicles, olive oil (1 ml/kg) or saline (0.9%). Statistical, gene ontology and supervised analysis clearly demonstrated the progression from acute effects, biological responses to and recovery from the treatments. Key biological processes disturbed by the individual treatments were characterised by gene ontology analyses and individual toxicant-responsive genes and pathways were identified by supervised analyses. Responses to the polyaromatic and chlorinated aromatic compounds showed a degree of commonality but were distinguishable and they were clearly segregated from the responses to the pro-oxidants cadmium and the organic hydroperoxide, as well as from the peroxisomal proliferator, perfluoro-octanoic acid. This study demonstrated the utility of the microarray technique in the identification of toxicant-responsive genes and in discrimination between modes of toxicant action.
authors.affiliation: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. t.d.williams@bham.ac.uk
authors.forename: Tim D
authors.initials: TD
authors.lastname: Williams
authors.forename: Amer
authors.initials: A
authors.lastname: Diab
authors.forename: Fernando
authors.initials: F
authors.lastname: Ortega
authors.forename: Victoria S
authors.initials: VS
authors.lastname: Sabine
authors.forename: Rita E
authors.initials: RE
authors.lastname: Godfrey
authors.forename: Francesco
authors.initials: F
authors.lastname: Falciani
authors.forename: J Kevin
authors.initials: JK
authors.lastname: Chipman
authors.forename: Stephen G
authors.initials: SG
authors.lastname: George
mesh_terms: D000818:Animals; D005432:Flounder / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000235:genetics; D008099:Liver / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D025341:Principal Component Analysis; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical