title: [Interaction of signal cascades induced by cAMP and prolactin in bovine oocyte-cumulus complexes].
abstract: Prolactin (PRL) is one of the pituitary hormones participating in the control of mammalian folliculo- and oogenesis. In the present study, the joint effect of PRL (50 ng/ml) and dibutyryl cAMP (dbcAMP, 1 mM) on oocyte maturation and the morphologic-functional state of surrounding cumulus cells was investigated in vitro. It has been shown that PRL totally suppresses the braking impact of dbcAMP on meiosis reinitiation and the completion of oocyte nuclear maturation. Furthermore, PRL partly inhibited cumulus expansion induced by dbcAMP, although it exerted the opposite effect in the control medium. In the presence of PRL, the inhibitory impact of dbcAMP on the proliferative activity of cumulus cells and on the PRL-elicited braking of destructive processes in the cells has been found. In cumulus cells, mRNA expression of PRL receptor long isoform was revealed by the RT-PCR method. The data obtained suggest an interaction of signal cascades induced by PRL and cAMP in bovine oocyte-cumulus complexes, with the coupling site of these cascades in oocytes being apparently different from that in cumulus cells.
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Lebedeva
authors.forename: G N
authors.initials: GN
authors.lastname: Singina
authors.forename: N A
authors.initials: NA
authors.lastname: Volkova
authors.forename: A N
authors.initials: AN
authors.lastname: Mormyshev
authors.forename: A K
authors.initials: AK
authors.lastname: Golubev
authors.forename: N A
authors.initials: NA
authors.lastname: Zinov'eva
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009866:Oogenesis / Q000187:drug effects*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D011981:Receptors, Prolactin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D015290:Second Messenger Systems / Q000187:drug effects; D013806:Theophylline / Q000037:antagonists & inhibitors / Q000494:pharmacology
chemical_list: D010726:Phosphodiesterase Inhibitors; D012333:RNA, Messenger; D011981:Receptors, Prolactin; D003994:Bucladesine; D011388:Prolactin; D013806:Theophylline