A subcortical maternal complex essential for preimplantation mouse embryogenesis.
We have identified a subcortical maternal complex (SCMC) that assembles during oocyte growth and is essential for zygotes to progress beyond the first embryonic cell divisions. At least four maternally encoded proteins contribute to this MDa complex: FLOPED, MATER, and TLE6 interact with each other while Filia binds independently to MATER. Although the transcripts encoding these proteins are degraded during meiotic maturation and ovulation, the SCMC proteins persist in the early embryo. The SCMC, located in the subcortex of eggs, is excluded from regions of cell-cell contact in the cleavage-stage embryo and segregates to the outer cells of the morulae and blastocyst. Floped(tm/tm) and/or Mater(tm/tm) eggs lack the SCMC but can be fertilized. However, these embryos do not progress beyond cleavage stage development and female mice are sterile. The proteins are conserved in humans, and similar maternal effect mutations may result in recurrent embryonic loss.
Li
Lei
L
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: lile@mail.nih.gov.
Baibakov
Boris
B
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA.
Dean
Jurrien
J
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA.
D000818:Animals; D000941:Antigens / Q000235:genetics / Q000378:metabolism*; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D056970:Co-Repressor Proteins; D004527:Egg Proteins / Q000235:genetics / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D005306:Fertilization; D006801:Humans; D007247:Infertility, Female / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D046912:Multiprotein Complexes / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology*; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D011247:Pregnancy; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
D000941:Antigens; D056970:Co-Repressor Proteins; D004527:Egg Proteins; D046912:Multiprotein Complexes; C416503:Nalp5 protein, mouse; D011506:Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C434948:Tle6 protein, mouse; C532243:filia protein, mouse