Effect of thymoquinone on ethylene glycol-induced kidney calculi in rats.
BACKGROUND
The aim of this study was to investigate the effects of thymoquinone, a major component of Nigella Sativa seeds on ethylene glycol-induced kidney calculi in rats.


METHODS
Sixty male Wistar rats were randomly divided into 6 groups (intact control, ethylene glycol control, and 4 experimental groups) and treated for 28 days according to the protocol of the study. The rats in experimental groups received ethylene glycol and intraperitoneal injection of thymoquinone either from the first day of the study or the 15th day, with either doses of 5 mg/kg or 10 mg/kg. Blood and 24-hour urine samples were collected at baseline and on day 28. Urine oxalate and citrate and serum electrolytes were also measured. On day 29, all rats were decapitated and their kidney specimens were studied.


RESULTS
On day 28, urine oxalate concentration significantly decreased in the experimental groups compared to the ethylene glycol group (P < .001). Also, serum calcium levels were significantly higher in the experimental groups (P = .001). Calcium oxalate deposits were smaller in the experimental groups than the ethylene glycol group. The mean number of deposits was lower in these groups, too (P < .001). Treatment with the lower dose of thymoquinone was associated with fewer deposits.


CONCLUSIONS
Thymoquinone significantly decreased the number and size of calcium oxalate deposits in the renal tubules. The dose and duration of treatment, however, does not have a linear relation with the outcomes. Further studies on thymoquinone as a preventive and therapeutic drug for kidney calculi are suggested.
Hadjzadeh
Mousa-Al-Reza
MA
Department of Physiology, Mashhad University of Medical Sciences, Mashhad, Iran. hajzadehmr@mums.ac.ir
Mohammadian
Nama
N
Rahmani
Zeynab
Z
Rassouli
Fatemeh Behnam
FB
D000818:Animals; D016227:Benzoquinones / Q000008:administration & dosage / Q000627:therapeutic use*; D002129:Calcium Oxalate; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D019855:Ethylene Glycol; D007274:Injections, Intraperitoneal; D007669:Kidney Calculi / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D008297:Male; D031881:Nigella sativa; D051381:Rats; D017208:Rats, Wistar
D016227:Benzoquinones; D002129:Calcium Oxalate; D019855:Ethylene Glycol; C003466:thymoquinone