title: Effects of antalarmin, a CRF receptor 1 antagonist, on fright reaction and endocrine stress response in crucian carp (Carassius carassius).
abstract: The corticotrophin-releasing factor (CRF) receptors show striking homogeneity throughout the vertebrate subphylum. In mammals, the CRF(1) receptor (CRFR(1)) plays an important role in mediating behavioral and endocrine responses to fear and stress. The specific roles of this receptor subtype in fear and stress reactions in non-mammalian vertebrates are largely unknown. Crucian carp displays the olfactory-mediated fright reaction, a stereotypic behavioral response to waterborne cues from damaged skin of conspecifics. This reaction shows several similarities to basic components of avoidance behavior in mammals. In the present study, we applied the non-peptide CRFR(1) antagonist, antalarmin, to crucian carp 1 h before exposure to conspecific skin extract. This treatment resulted in a suppression of the fright reaction. After skin extract exposure, antalarmin treatment also lead to lower plasma cortisol values, as compared to vehicle treatment. This suppression of the behavioral fright reaction and the stress induced rise in plasma cortisol in crucian carp suggests that the functions of the CRFR(1) are conserved by evolution.
authors.affiliation: Department of Molecular Biosciences, University of Oslo, Blindern, 0316, Oslo, Norway.
authors.forename: Stine
authors.initials: S
authors.lastname: Lastein
authors.forename: Erik
authors.initials: E
authors.lastname: Höglund
authors.forename: Oyvind
authors.initials: O
authors.lastname: Overli
authors.forename: Kjell B
authors.initials: KB
authors.lastname: Døving
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D002347:Carps / Q000502:physiology*; D005239:Fear; D006854:Hydrocortisone / Q000097:blood*; D009043:Motor Activity / Q000187:drug effects; D011743:Pyrimidines / Q000494:pharmacology*; D011758:Pyrroles / Q000494:pharmacology*; D018019:Receptors, Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors* / Q000502:physiology; D012903:Smell; D013239:Stereotyped Behavior / Q000187:drug effects; D013312:Stress, Physiological / Q000187:drug effects*; D000099052:CRF Receptor, Type 1
chemical_list: D011743:Pyrimidines; D011758:Pyrroles; D018019:Receptors, Corticotropin-Releasing Hormone; C103016:antalarmin; D000099052:CRF Receptor, Type 1; D006854:Hydrocortisone