title: BAG3 protein regulates caspase-3 activation in HIV-1-infected human primary microglial cells.
abstract: BAG3, a member of the BAG co-chaperones family, is expressed in several cell types subjected to stressful conditions, such as exposure to high temperature, heavy metals, drugs. Furthermore, it is constitutively expressed in some tumors. Among the biological activities of the protein, there is apoptosis downmodulation; this appears to be exerted through BAG3 interaction with the heat shock protein (Hsp) 70, that influences cell apoptosis at several levels. We recently reported that BAG3 protein was detectable in the cytoplasm of reactive astrocytes in HIV-1-associated encephalopathy biopsies. Here we report that downmodulation of BAG3 protein levels allows caspase-3 activation by HIV-1 infection in human primary microglial cells. This is the first reported evidence of a role for BAG3 in the balance of death versus survival during viral infection.
authors.affiliation: Department of Pharmaceutical Sciences (DiFarma), University of Salerno, Salerno, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Rosati
authors.forename: Kamel
authors.initials: K
authors.lastname: Khalili
authors.forename: Satish L
authors.initials: SL
authors.lastname: Deshmane
authors.forename: Sujatha
authors.initials: S
authors.lastname: Radhakrishnan
authors.forename: Maria
authors.initials: M
authors.lastname: Pascale
authors.forename: M Caterina
authors.initials: MC
authors.lastname: Turco
authors.forename: Liberato
authors.initials: L
authors.lastname: Marzullo
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins; D001921:Brain / Q000166:cytology / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism*; D002478:Cells, Cultured; D004789:Enzyme Activation; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D017628:Microglia / Q000201:enzymology* / Q000821:virology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C414431:BAG3 protein, human; D053148:Caspase 3