[Construction and characterization of a novel somatostatin prokaryotic expression].
In the current work, the fusion gene including somatostatin (SS) and the hepatitis B surface antigen gene was cloned into a balanced lethal system plasmid (pYA3493), and then transformed into asd- attenuated Salmonella choleraesuis C500 strain, the positive transformant without antibiotic resistance gene was confirmed by restriction analysis and DNA sequencing, designated as pYA-SS. The expression and immunogenicity of fusion protein were detected by SDS-PAGE and Western blot analysis. These results show that the recombinant prokaryotic expression plasmid pYA-SS could express the SS fusion protein with good immunogenicity in C500 strain. In above all, this study could provide reliable materials to develop novel, good and safe vaccine in enhancing the growth of animals.
Liang
Aixin
A
College of Animal Science &Technology, Huazhong Agricultural University, Wuhan 430070, China.
Feng
Xigang
X
Han
Li
L
Hua
Guohua
G
Sang
Lei
L
Liu
Xingbin
X
Liu
Yun
Y
Yang
Liguo
L
D000818:Animals; D019677:Artificial Gene Fusion; D003001:Cloning, Molecular; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011387:Prokaryotic Cells / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D012476:Salmonella arizonae / Q000235:genetics* / Q000378:metabolism; D013004:Somatostatin / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*
D006514:Hepatitis B Surface Antigens; D011993:Recombinant Fusion Proteins; D013004:Somatostatin