title: Giant magnetoresistance sensors. 1. Internally calibrated readout of scanned magnetic arrays.
abstract: This paper describes efforts aimed at setting the stage for the application of giant magnetoresistance sensor (GMRs) networks as readers for quantification of biolytes selectively captured and then labeled with superparamagnetic particles on a scanned chip-scale array. The novelty and long-range goal of this research draws from the potential development of a card-swipe instrument through which an array of micrometer-sized, magnetically tagged addresses (i.e., a sample stick) can be interrogated in a manner analogous to a credit card reader. This work describes the construction and testing of a first-generation instrument that uses a GMR sensor network to read the response of a "simulated" sample stick. The glass sample stick is composed of 20-nm-thick films of permalloy that have square or rectangular lateral footprints of up to a few hundred micrometers. Experiments were carried out to gain a fundamental understanding of the dependence of the GMR response on the separation between, and planarity of, the scanned sample stick and sensor. Results showed that the complex interplay between these experimentally controllable variables strongly affect the shape and magnitude of the observed signal and, ultimately, the limit of detection. This study also assessed the merits of using on-sample standards as internal references as a facile means to account for small variations in the gap between the sample stick and sensor. These findings were then analyzed to determine various analytical figures of merit (e.g., limit of detection in terms of the amount of magnetizable material on each address) for this readout strategy. An in-depth description of the first-generation test equipment is presented, along with a brief discussion of the potential widespread applicability of the concept.
authors.affiliation: Department of Chemistry, Ames Laboratory--USDOE, and Institute for Combinatorial Discovery, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: John
authors.initials: J
authors.lastname: Nordling
authors.forename: Rachel L
authors.initials: RL
authors.lastname: Millen
authors.forename: Heather A
authors.initials: HA
authors.lastname: Bullen
authors.forename: Marc D
authors.initials: MD
authors.lastname: Porter
authors.forename: Mark
authors.initials: M
authors.lastname: Tondra
authors.forename: Michael C
authors.initials: MC
authors.lastname: Granger