title: Expression and secretion of N-acylethanolamine-hydrolysing acid amidase in human prostate cancer cells.
abstract: N-acylethanolamines (NAEs) are a class of bioactive lipid molecules in animal tissues, including the endocannabinoid anandamide and the anti-inflammatory substance N-palmitoylethanolamine. Enzymatic hydrolysis of NAEs is considered to be an important step to regulate their endogenous levels. Lysosomal NAE-hydrolysing acid amidase (NAAA) as well as fatty acid amide hydrolase (FAAH) is responsible for this reaction. Here, we report relatively high expression of NAAA in human prostate cancer cells (PC-3, DU-145 and LNCaP) and prostate epithelial cells (PrEC), with the highest mRNA level in LNCaP cells. FAAH and the NAE-forming enzyme N-acylphosphatidylethanolamine-hydrolysing phospholipase D (NAPE-PLD) were also detected in these cells. NAAA activity in LNCaP cells could be distinguished from coexisting FAAH activity, based on their different pH dependency profiles and specific inhibition of FAAH activity by URB597. These results showed that both the enzymes were functionally active. We also found that NAAA was partly secreted from LNCaP cells, which underlined possible usefulness of this enzyme as a biomarker of prostate cancer.
authors.affiliation: Department of Biochemistry, Kagawa University School of Medicine, Kagawa, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Zhao
authors.forename: Toru
authors.initials: T
authors.lastname: Uyama
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tsuboi
authors.forename: Xiu-Xian
authors.initials: XX
authors.lastname: Wu
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kakehi
authors.forename: Natsuo
authors.initials: N
authors.lastname: Ueda
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D063385:Cannabinoid Receptor Modulators / Q000378:metabolism; D045744:Cell Line, Tumor; D063388:Endocannabinoids; D004983:Ethanolamines / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D053284:Polyunsaturated Alkamides / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000378:metabolism; D014018:Tissue Distribution; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000581:Amidohydrolases; D001095:Arachidonic Acids; D063385:Cannabinoid Receptor Modulators; D063388:Endocannabinoids; D004983:Ethanolamines; D053284:Polyunsaturated Alkamides; D000099252:Fatty Acid Amide Hydrolases; C022203:N-acylethanolamines; C523108:NAAA protein, human; C078814:anandamide