title: Identification of Fructose-1,6-bisphosphate aldolase cytosolic class I as an NMH7 MADS domain associated protein.
abstract: We are interested in identifying proteins that interact with the MADS domain protein NMH7 of Medicago sativa. We use an affinity column with a synthetic peptide derived from the MADS domain of NMH7 which has been reported to mediate protein-protein interaction with non-MADS domain interacting proteins. We identified approximately 40 and approximately 80kDa specifically bound proteins as the monomeric and dimeric forms of Fructose-1,6-bisphosphate aldolase cytosolic class I. NiNTA pull down assays revealed that K- and C-terminus regions of NMH7 are not required for the interaction with aldolase. Aldolase enzymatic activity is not required for the interaction with NMH7. NMH7 and aldolase were coimmunoprecipitated from non-inoculated seed and seedlings extracts. Colocalization studies using confocal microscopy showed that aldolase and NMH7 are localized in the cytoplasm and the nucleus of the cortical cells. These data together show that M. sativa aldolase is a novel MADS domain binding protein, and suggest a broader functional repertory for this enzyme, as has been proposed for other glycolytic enzymes.
authors.affiliation: Departamento de Ecología Funcional, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad Universitaria 04510, México DF, Mexico.
authors.forename: Julio
authors.initials: J
authors.lastname: Páez-Valencia
authors.forename: Pedro
authors.initials: P
authors.lastname: Valencia-Mayoral
authors.forename: Concepción
authors.initials: C
authors.lastname: Sánchez-Gómez
authors.forename: Alejandra
authors.initials: A
authors.lastname: Contreras-Ramos
authors.forename: Ismael
authors.initials: I
authors.lastname: Hernández-Lucas
authors.forename: Eleazar
authors.initials: E
authors.lastname: Martínez-Barajas
authors.forename: Alicia
authors.initials: A
authors.lastname: Gamboa-DeBuen
mesh_terms: D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006019:Glycolysis; D026161:MADS Domain Proteins / Q000378:metabolism*; D000455:Medicago sativa / Q000201:enzymology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism*; D036226:Seedlings / Q000201:enzymology; D012639:Seeds / Q000201:enzymology
chemical_list: D026161:MADS Domain Proteins; D010940:Plant Proteins; D005634:Fructose-Bisphosphate Aldolase