Biodegradation of chlorpyrifos in soil by enriched cultures.
Three aerobic bacterial consortia, AC, BC, and DC, developed from pesticide-contaminated soils of Punjab were able to degrade chlorpyrifos after 21 days of incubation in basal medium by 54, 46, and 61% and chlorpyrifos (50 mg/L) in soil after 30 days by 50, 56, and 64%. Pseudomonas aeruginosa, Bacillus cereus, Klebsiella sp., and Serratia marscecens obtained from these consortia showed 84, 84, 81, and 80% degradation of chlorpyrifos (50 mg/L) in liquid medium after 20 days and 92, 60, 56, and 37% degradation of chlorpyrifos (50 mg/L) in soil after 30 days. Populations of Bacillus cereus, Klebsiella sp., and Serratia marscecens remained steady in soil experiments except for P. aeruginosa, where the population showed a substantial increase. Formation of 3,5,6-trichloro-2-pyridinol, the major metabolite of chlorpyrifos degradation, was observed during the degradation of chlorpyrifos by P. aeruginosa, which disappeared to negligible amounts.
Lakshmi
C Vidya
CV
Department of Biotechnology and Environmental Sciences, Thapar Institute of Engineering and Technology, Patiala, 147004, Punjab, India.
Kumar
Mohit
M
Khanna
Sunil
S
D001420:Bacteria, Aerobic / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004390:Chlorpyrifos / Q000378:metabolism*; D011728:Pyridones / Q000378:metabolism; D012988:Soil Microbiology; D013997:Time Factors
D011728:Pyridones; C012587:3,5,6-trichloro-2-pyridinol; D004390:Chlorpyrifos