Distribution and structural behavior of hemoglobin between the two phases in SDS/n-C5H11OH/H2O system.
In the sodium dodecyl sulfate (SDS)/n-pentanol (n-C5H11OH)/water (H2O) system, LL is the coexisting region of W/O and O/W or bicontinuous microemulsions. In the LL region, the system separates into two phases (the upper phase and the lower phase) spontaneously. The distribution and structural behavior of hemoglobin (Hb) between the upper and lower phases in SDS/n-C5H11OH/H2O system were studied by UV-vis and circular dichroism (CD) spectroscopy. The results indicate that different structures of the two phases bring the conformational change of Hb. The addition of Hb results in the redistribution of SDS between the upper and lower phases.
Chen
Yuan
Y
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, People's Republic of China.
Guo
Rong
R
D002942:Circular Dichroism; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification; D000439:Pentanols; D012967:Sodium Dodecyl Sulfate; D013056:Spectrophotometry, Ultraviolet; D014867:Water
D006454:Hemoglobins; D000439:Pentanols; D014867:Water; D012967:Sodium Dodecyl Sulfate