title: Self-assembly of quantum dots and carbon nanotubes for ultrasensitive DNA and antigen detection.
abstract: A highly selective, ultrasensitive, fluorescence detection method for DNA and antigen based on self-assembly of multiwalled carbon nanotubes (CNTs) and CdSe quantum dots (QDs) via oligonucleotide hybridization is reported. Mercaptoalkyloligonucleotide molecules bind to the quantum dots, while amineoalkyloligonucleotides bind to CNTs with -COCl surface groups. QDs and CNTs further assemble into nanohybrids through DNA hybridization in the presence of target complementary oligonucleotides. The method is achieved with good repeatability with the detection limit of 0.2 pM DNA molecules and 0.01 nM antigen molecules. This novel detection system can also be used for multicomponent detection and antigen-antibody immunoreaction. The novel system has great potential in applications such as ultrasensitive pathogen DNA or antigen or antibody detection, molecular imaging, and photoelectrical biosensors.
authors.affiliation: Department of Institute of Micro-Nano Science and Technology, Shanghai JiaoTong University, PR China. dxcui@sjtu.edu.cn
authors.forename: Daxiang
authors.initials: D
authors.lastname: Cui
authors.forename: Bifeng
authors.initials: B
authors.lastname: Pan
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Rina
authors.initials: R
authors.lastname: Wu
authors.forename: Jingping
authors.initials: J
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Toru
authors.initials: T
authors.lastname: Asahi
mesh_terms: D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D045663:Quantum Dots; D013379:Substrate Specificity
chemical_list: D000906:Antibodies; D000941:Antigens; D037742:Nanotubes, Carbon; D004247:DNA