Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor microarrays.
We describe the development and operation of a two-laser, large-field hyperspectral scanner for analysis of multicolor genotyping microarrays. In contrast to confocal microarray scanners, in which wavelength selectivity is obtained by positioning band-pass filters in front of a photomultiplier detector, hyperspectral microarray scanners collect the complete visible emission spectrum from the labeled microarrays. Hyperspectral scanning permits discrimination of multiple spectrally overlapping fluorescent labels with minimal use of optical filters, thus offering important advantages over standard filter-based multicolor microarray scanners. The scanner uses two-sided oblique line illumination of microarrays. Two lasers are used for the excitation of dyes in the visible and near-infrared spectral regions. The hyperspectral scanner was evaluated with commercially available two-color calibration slides and with in-house-printed four-color microarrays containing dyes with spectral properties similar to their commercial genotyping array counterparts.
Erfurth
Florian
F
Department of Photonics and Sensors, Innovative Bio-, Medical- and Environmental Technologies (GMBU e.V.), Felsbachstrasse 7 D-07745 Jena, Germany. erfurth@gmbu-jena.de
Tretyakov
Alexander
A
Nyuyki
Berla
B
Mrotzek
Grit
G
Schmidt
Wolf-Dieter
WD
Fassler
Dieter
D
Saluz
Hans Peter
HP
D002138:Calibration; D003116:Color; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D005838:Genotype; D007834:Lasers; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D013499:Surface Properties
D005456:Fluorescent Dyes