[Analysis of the temporal organization of the synthesis of RNA and protein in blood lymphocytes during the immune response of the body].
The temporal organization of the synthesis of RNA and antibodies in immunocompetent cells has been investigated by fluorecsent spectroscopy. Two fluorochromes: acridine orange and 1-anilino-8-naphthalenesulfonate were used. Temporal changes in the mean values of the characteristic parameters alpha' and beta in blood lymphocytes stained with acridine orange and 1-anilino-8-naphthalenesulfonate in the immune reaction to the introduction of ovalbumin were obtained. It was shown that the maximal increase in RNA and protein synthesis in blood lymphocytes by a factor of 6-8 occurs after the fourth immunization. The simultaneous increase in RNA and protein synthesis in cells was indicative of cell activation. A general response of immunocompetent blood cells to the introduction of the antigen was observed. The results confirm the idea about the cooperative interaction between different lymphocyte subpopulations during the synthesis of antibodies.
Karnaukhova
N A
NA
Sergievich
L A
LA
Karnaukhov
V N
VN
D000165:Acridine Orange / Q000494:pharmacology; D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation / Q000502:physiology*; D000941:Antigens / Q000276:immunology / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000502:physiology*; D012313:RNA / Q000096:biosynthesis* / Q000276:immunology; D011817:Rabbits; D013050:Spectrometry, Fluorescence / Q000379:methods
D000817:Anilino Naphthalenesulfonates; D000941:Antigens; D005456:Fluorescent Dyes; C027132:1-anilino-8-naphthalenesulfonate; D012313:RNA; D000165:Acridine Orange