The effect of levamisole on sheep trachea alone and with trichlorfon combination.
The aim of this study is to determine the effect of levamisole and levamisole-trichlorfon combination on isolated sheep trachea. Contraction was achieved with levamisole concentrations (10(-8)-10(-3) M) on tracheal strips of adult sheep (> 1 year of age). Pretreatment with trichlorfon (10(-7) M)-levamisole (10(-7) M) decreased the pD2 and E(max) of Ach when compared to levamisole pretreatment (p < or = 0.01). Pretreatment with levamisole (10(-7) M), decreased the E(max) (p < or = 0.01) and pD2 (p < 0.05) of bethanechol concentrations (10(-8)-10(-3) M) significantly. Pretreatment with atropine (10(-6) M) decreased the E(max) of levamisole (10(-4) M) significantly (p < 0.05). To conclude, levamisole acted mainly on the muscarinic receptors of the sheep trachea and this effect was partly inhibited by atropine. Adverse interaction was present between levamisole and trichlorfon.
Baydan
Emine
E
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
Yildirim
Ebru
E
Ince
Sinan
S
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D018723:Bethanechol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D066298:In Vitro Techniques; D007978:Levamisole / Q000494:pharmacology*; D008464:Mecamylamine / Q000494:pharmacology; D009538:Nicotine / Q000494:pharmacology; D034561:Sheep, Domestic; D014132:Trachea / Q000187:drug effects*; D014236:Trichlorfon / Q000494:pharmacology*
D018723:Bethanechol; D007978:Levamisole; D008464:Mecamylamine; D009538:Nicotine; D014236:Trichlorfon; D000109:Acetylcholine