Antioxidative and cytoprotective effects of Artemisia capillaris fractions.
The antioxidant effects of Artemisia capillaris fractions against reactive oxygen species (ROS) were evaluated by measuring scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide (O_2(-)), hydroxyl (HO.) and nitric oxide (NO.) radical. Among five solvent fractions, ethyl acetate fraction showed the highest total polyphenol and total flavonoid contents as 648.75 and 89.09 microg/mg, respectively. Also, the ethyl acetate fraction showed the highest scavenging activity; the 50% inhibitory concentration (IC50, microg/mg) value for DPPH, O_2(-), HO. and NO. radical scavenging were 4.76, 31.54, 69.34 and 74.63, respectively. Additionally, the highest inhibition of rat liver microsomal lipid peroxidation was observed by ethyl acetate fraction. Except for free radical-mediated protein damage, ethyl acetate fraction showed the highest scavenging activity. The effect of Artemisia capillaris fractions on cell viability and DNA damage induced by H2O2 in Raw 264.7 cell were also evaluated by MTT and comet assay, respectively. The protective effect of ethyl acetate fraction, as indicated by cell viability increasing 71% and DNA breakage decreasing 51% as compared with H2O2-treated positive control. These results suggest that ethyl acetate fraction possess significant ROS scavenging and protective effect against oxidative DNA damage.
Hong
Jung-Hee
JH
The Center for Traditional Microorganism Resources, Keimyung University, 1000 Shindang-dong, Dalseo-gu, Daegu, Korea.
Lee
Ji-Won
JW
Park
Jung-Hyun
JH
Lee
In-Seon
IS
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry*; D001713:Biphenyl Compounds; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D019610:Cytoprotection; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017665:Hydroxyl Radical; D008264:Macrophages; D051379:Mice; D009000:Monocytes; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D013481:Superoxides / Q000037:antagonists & inhibitors
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D013481:Superoxides; D009569:Nitric Oxide; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl