[Inhibitory effects of hybrid liposomes on the growth of gastric tumor cells established from cotton rats].
We established cell-line (CoRa 622 G6) of gastric carcinoma using cotton rats with spontaneous malignant gastric carcinoma with hypergastrinaemia. Inhibitory effects of hybrid liposomes (HL) composed of dimyristoylphosphatidylcoline (DMPC) and polyoxyethylene (n) dodecyl ether (C(12)(EO)(n): n=21, 23, 25) on the growth of CoRa 622 G6 cells were clarified on the basis of WST-1 assay. Fusion and accumulation of HL including fluorescence probe into CoRa 622 G6 cell membrane were clarified using confocal laser microscopy and total internal reflection fluorescence microscopy. Induction of apoptosis of CoRa 622 G6 cells after the treatment with HL was observed in fluorescence micrographs on the basis of Annexin-V binding assay and TUNEL method using confocal laser microscopy. The results in this study could contribute to the chemotherapy for patients with gastric carcinoma.
Matsuoka
Yusuke
Y
Division of Applied Life Science, Graduate School of Engineering, Sojo University, Japan.
Nagata
Miho
M
Komizu
Yuji
Y
Ichihara
Hideaki
H
Kawase
Shiro
S
Sawada
Yukiharu
Y
Matsumoto
Yoko
Y
Ueoka
Ryuichi
R
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D004134:Dimyristoylphosphatidylcholine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D008081:Liposomes / Q000494:pharmacology*; D008297:Male; D018613:Microscopy, Confocal; D016205:Sigmodontinae; D013274:Stomach Neoplasms / Q000473:pathology* / Q000648:ultrastructure
D008081:Liposomes; D004134:Dimyristoylphosphatidylcholine