title: Cellular uptake of gold nanoparticles directly cross-linked with carrier peptides by osteosarcoma cells.
abstract: Nanoparticles have been extensively used for a variety of biomedical applications and there is a growing need for highly specific and efficient delivery of the nanoparticles into target cells and subcellular location. We attempted to accomplish this goal by modifying gold particles with peptide motif's that are known to deliver a 'cargo' into chosen cellular location specifically, we intended to deliver nanogold particles into cells through chemical cross-linking with different peptides known to carry protein into cells. Our results suggest that specific sequence of such 'carrier peptides' can efficiently deliver gold nanoparticles into cells when chemically cross-linked with the metal particles.
authors.affiliation: Department of Orthopedics, Mayo Clinic and Foundation, Rochester, MN 55905, USA. ddmandal@yahoo.com
authors.forename: Deendayal
authors.initials: D
authors.lastname: Mandal
authors.forename: Avudaippan
authors.initials: A
authors.lastname: Maran
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Yaszemski
authors.forename: Mark E
authors.initials: ME
authors.lastname: Bolander
authors.forename: Gobinda
authors.initials: G
authors.lastname: Sarkar
mesh_terms: D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000737:chemistry / Q000493:pharmacokinetics; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000737:chemistry* / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D003432:Cross-Linking Reagents; D016503:Drug Delivery Systems; D006046:Gold / Q000737:chemistry* / Q000493:pharmacokinetics*; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals / Q000737:chemistry / Q000493:pharmacokinetics; D012516:Osteosarcoma / Q000378:metabolism* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry* / Q000493:pharmacokinetics*; D013347:Subcellular Fractions / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D019913:Nuclear Localization Signals; D010455:Peptides; D006046:Gold