title: Berberine inhibits SDF-1-induced AML cells and leukemic stem cells migration via regulation of SDF-1 level in bone marrow stromal cells.
abstract: Berberine plays a prominent role on the control of tumor cell invasion and migration. SDF-1 is a homeostatic chemokine that signals through CXCR4 which is expressed by hematopoietic tumor cells. The SDF-1/CXCR4 axis is involved in the migration process of leukemic cells. In this study, we investigated the effects of berberine on the SDF-1-induced HL-60 cells, primary acute myeloid leukemia (AML) cells and leukemic stem cells (LSCs) migration. Transwell migration chambers (8 microm) were used to assess the role of berberine on leukemic cell migration; Flow cytometry was used to analyze the role of berberine on the CXCR4 expression; SDF-1 protein level secreted by bone marrow stromal cells (BMSCs) was evaluated by ELISA. Results demonstrated that berberine could partly inhibit SDF-1-induced AML cells as well as LSCs migration. Berberine could reduce SDF-1 protein level secreted by BMSCs in the microenvironment but not affect CXCR4 expression on HL-60 cell membrane, and we hypothesized that berberine could inhibit AML cells migration partly by reducing the secreting of SDF-1 by BMSCs and inhibiting HERG1 K(+) channels of leukemic cells. Therefore, it is speculated that berberine might be a potentially effective agent for prevention of leukemia.
authors.affiliation: Center for Stem Cell Research and Application, Union Hospital of Huazhong University of Science and Technology, 1277# Jiefang Avenue, Wuhan, Hubei 430022, PR China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Li
authors.forename: Linlin
authors.initials: L
authors.lastname: Guo
authors.forename: Shenghua
authors.initials: S
authors.lastname: Jie
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.forename: Wen
authors.initials: W
authors.lastname: Du
authors.forename: Lingling
authors.initials: L
authors.lastname: Fan
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.forename: Bingjie
authors.initials: B
authors.lastname: Fu
authors.forename: Shiang
authors.initials: S
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001599:Berberine / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000502:physiology; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000378:metabolism*; D002648:Child; D003864:Depression, Chemical; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000502:physiology
chemical_list: D016588:Anticarcinogenic Agents; D054377:Chemokine CXCL12; D001599:Berberine