CD36 is significantly correlated with adipophilin in human carotid lesions and inversely correlated with plasma ApoAI.
OxLDL uptake and cholesterol efflux inhibition in macrophages play a key role in atherosclerotic plaque formation, rupture, and thrombotic ischemia. This study investigates genes implicated in OxLDL uptake (CD36, SRA), cholesterol efflux inhibition (adipophilin, ADFP), and inflammatory recruitments of leukocytes (IL-8) in plaque lesion areas (PLAs) compared to nonplaque lesion areas (NPLAs) in human carotid endarterectomy specimens. Gene and protein expressions were assayed using quantitative PCR and quantitative immunohistochemistry. Pearson tests were used to investigate potential correlation between (a) different gene expressions and (b) gene expression and patient's plasma constituents. CD36, SRA, ADFP, and IL-8 were shown to be significantly more expressed in PLA compared to NPLA. In PLA, a significant correlation was observed between CD36, SRA, ADFP, and IL-8 mRNA levels. Moreover, CD36 expression level was significantly inversely correlated to plasma marker ApoAI. The above investigated genes/proteins may play a key role in the maturation of atherosclerotic lesions.
Collot-Teixeira
Sophie
S
Cardiovascular Division, King's College London, University of London, London, UK. sophie.collot@kcl.ac.uk
Barbatis
Calypso
C
Bultelle
Florence
F
Koutouzis
Michael
M
Pasterkamp
Gerard
G
Fraser
Paul
P
Kyriakides
Zenon S
ZS
Poston
Robin N
RN
Ristagno
Angelique
A
McGregor
Lilian
L
Boulanger
Chantal M
CM
Leseche
Guy
G
McGregor
John L
JL
D016632:Apolipoprotein A-I / Q000097:blood*; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D016893:Carotid Stenosis / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D008076:Cholesterol, HDL / Q000097:blood; D015536:Down-Regulation / Q000187:drug effects; D016894:Endarterectomy, Carotid; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D000070780:Perilipin-2; D012333:RNA, Messenger / Q000032:analysis; D051117:Scavenger Receptors, Class A / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D016632:Apolipoprotein A-I; D018955:CD36 Antigens; D008076:Cholesterol, HDL; D016209:Interleukin-8; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; C000602982:PLIN2 protein, human; D000070780:Perilipin-2; D012333:RNA, Messenger; D051117:Scavenger Receptors, Class A; C099252:oxidized low density lipoprotein