[Involvement of bacterial microflora in etiopathogenesis of chronic sinusitis].
We used the method of amplification of RNA 16S genes in combination with discriminating hybridization of amplification products with species-specific probes in DNA-chip format to identify bacterial flora in patients with chronic sinusitis. A total of 34 samples from 33 patients suffering from chronic sinusitis have been tested. Biopsy specimens of sinus mucosa served as material for sinus microflora studies in the absence of sinusitis exacerbation. It was found that in the exacerbation-free period the samples contained no bacterial RNA 16S while human RNA was isolated from all the samples. Moreover, we isolated and typed fragments of 16S RNA E.fecalis genes from tested in parallel sample of the wall of the oroantral fistula. This confirmed significance and effectiveness of the above method. We came to the conclusion that sinus mucosa is sterile in chronic hyperplastic sinusitis.
Turovskiĭ
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D000293:Adolescent; D000328:Adult; D000368:Aged; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology*; D001706:Biopsy; D002908:Chronic Disease; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012329:RNA, Bacterial / Q000032:analysis*; D012852:Sinusitis / Q000382:microbiology*; D055815:Young Adult
D012329:RNA, Bacterial