Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus.
A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised five genes including cathepsin L-like cysteine peptidase, hypothetical proteins and unknown genes, was of particular interest because the transcripts increased rapidly (< or = 0.25 hours) and reached high expression levels in response to YHV injection. Microarray data were validated by realtime RT-PCR analyses of selected differentially expressed transcripts. In addition, comparative analysis of the hemocyte transcription levels of three of these genes between surviving and non-surviving shrimp revealed significantly higher expression levels in surviving shrimp.
Pongsomboon
Siriporn
S
Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Tang
Sureerat
S
Boonda
Suleeporn
S
Aoki
Takashi
T
Hirono
Ikuo
I
Yasuike
Motoshige
M
Tassanakajon
Anchalee
A
D000818:Animals; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006434:Hemocytes / Q000502:physiology / Q000821:virology; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D033561:Penaeidae / Q000166:cytology / Q000502:physiology / Q000821:virology; D046828:Roniviridae / Q000235:genetics / Q000378:metabolism*