title: RNA intramolecular dynamics by single-molecule FRET.
abstract: Investigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynamic and thermodynamic aspects of the folding process of a given RNA. Its application requires interdisciplinary work from the fields of chemistry, biochemistry, and physics. The present work gives detailed instructions on the synthesis of RNA molecules labeled with two fluorescent dyes interacting by FRET, as well as on their investigation by single-molecule fluorescence spectroscopy.
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hengesbach
authors.forename: Andrei
authors.initials: A
authors.lastname: Kobitski
authors.forename: Felix
authors.initials: F
authors.lastname: Voigts-Hoffmann
authors.forename: Carina
authors.initials: C
authors.lastname: Frauer
authors.forename: G Ulrich
authors.initials: GU
authors.lastname: Nienhaus
authors.forename: Mark
authors.initials: M
authors.lastname: Helm
mesh_terms: D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D007834:Lasers; D009690:Nucleic Acid Conformation; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D005456:Fluorescent Dyes; D012313:RNA