Relationship between nm23H1 genetic instability and clinical pathological characteristics in Chinese digestive system cancer patients.
OBJECTIVE
To study the relationship between nm23H1 gene genetic instability and its clinical pathological characteristics in Chinese digestive system cancer patients.


METHODS
Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to analyze the microsatellite instability (MSI) and loss of heterozygosity (LOH). Immunohistochemistry was employed to detect the expression of nm23H1.


RESULTS
The MSI was higher in TNM stage I + II than in stage III + IV of gastric, colonic and gallbladder carcinomas. The LOH was higher in TNM stage III + IV than in stage I + II of gastric, colonic and hepatocellular carcinomas. Lymphatic metastasis was also observed. The expression of nm23H1 protein was lower in TNM stage III + IV than in stage I + II of these tumors and in patients with lymphatic metastasis.The nm23H1 protein expression was higher in the LOH negative group than in the LOH positive group.


CONCLUSIONS
MSI and LOH may independently control the biological behaviors of digestive system cancers. MSI could serve as an early biological marker of digestive system cancers. Enhanced expression of nm23H1 protein could efficiently inhibit cancer metastasis and improve its prognosis. LOH mostly appears in late digestive system cancer.
Yang
Yue-Qin
YQ
Institute of Cell Biology, Zhejiang University, Hangzhou, Zhejiang Province, China.
Wu
Liang
L
Chen
Jin-Xing
JX
Sun
Jian-Zhong
JZ
Li
Meng
M
Li
Dong-Mei
DM
Lu
Hai-Ying
HY
Su
Zhi-Hong
ZH
Lin
Xin-Qiu
XQ
Li
Ji-Cheng
JC
D044466:Asian People / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000208:ethnology / Q000235:genetics; D002681:China; D003110:Colonic Neoplasms / Q000208:ethnology / Q000235:genetics; D004067:Digestive System Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D005706:Gallbladder Neoplasms / Q000208:ethnology / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D006801:Humans; D008113:Liver Neoplasms / Q000208:ethnology / Q000235:genetics; D019656:Loss of Heterozygosity; D008207:Lymphatic Metastasis; D053842:Microsatellite Instability; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*; D009367:Neoplasm Staging; D010641:Phenotype; D013274:Stomach Neoplasms / Q000208:ethnology / Q000235:genetics
D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human