[Advances in the MDCK-MDR1 cell model and its applications to screen drug permeability].
This article introduces the characteristics and establishment of MDCK-MDR1 cell line, and its applications as an in vitro model for the assessment of the membrane permeability properties of drugs. MDCK-MDR1 cell model is widely used in drug screenings, and the study of mechanisms of drug interaction and absorption or transport. The review summarizes the progress and applications of MDCK-MDR1 cell model. Because there is high polarized P-glycoprotein (P-gp) expression in MDCK-MDR1 cells, it could be used as a drug transport model to screen P-gp substrates or inhibitors, as well as models of intestinal mucosa, blood brain barrier and kidney. Although there are some disadvantages in MDCK-MDR1 cell model, it is an effective tool for implementing permeability of drug in an optimal fashion.
Liu
Yao
Y
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Zeng
Su
S
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D001812:Blood-Brain Barrier / Q000378:metabolism*; D018938:Caco-2 Cells; D002460:Cell Line; D002463:Cell Membrane Permeability; D004285:Dogs; D004353:Drug Evaluation, Preclinical; D018432:Drug Resistance, Multiple; D019450:Genes, MDR; D006801:Humans; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004364:Pharmaceutical Preparations