Optimization of diagnostic ELISA-based tests for the detection of auto-antibodies against tumor antigens in human serum.
Colorectal cancer is one of the most common cancer types worldwide and it continues to be a serious public health problem. Early detection and diagnosis are of great importance in cancer management. At present, diagnostic blood tests are based on the detection of tumor-associated markers such as carcino-embryonic antigen (CEA), the cancer antigen CA19-9 for gastrointestinal cancer, CA15-3 for breast cancer or CA125 for ovarian cancer. The lack of sensitivity and specificity of these markers prevents their general use in cancer screening of an average risk population. Therefore, new cancer biomarkers or better screening methods are necessary to improve the diagnostics of the disease. This study was directed to the optimization of a diagnostic, enzyme linked immunosorbent assay (ELISA) based test to identify and validate new serum markers, such as extracellular Protein Kinase A (ecPKA) and Nicotinamide N-Methyltransferase (NNMT). In this type of assay, the cancer antigens are quantified indirectly - by detecting the presence of auto-antibodies against tumor proteins in human serum. The result of the optimization and validation process was in the case of ecPKA a reproducible and stable assay. In case of NNMT the assay was probably not sensitive enough.
Stefatić
Daria
D
Division of Oncology, Department of Internal Medicine, Medical University of Graz, Graz, Austria.
Riederer
Monika
M
Balić
Marija
M
Dandachi
Nadia
N
Stanzer
Stefanie
S
Janesch
Birgit
B
Resel
Margit
M
Ler
Darko
D
Samonigg
Hellmut
H
Bauernhofer
Thomas
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D050903:Nicotinamide N-Methyltransferase / Q000276:immunology*; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; C494406:NNMT protein, human; D050903:Nicotinamide N-Methyltransferase; D017868:Cyclic AMP-Dependent Protein Kinases