Enhancement of natural killer cell activity in vitro against human tumor cells by some plants from Jordan.
The effect of different concentrations (0, 0.01, 0.1, and 0.5 mg/ml) of plant aqueous extracts on the anti-tumor activity of natural killer (NK) cells isolated from human blood was examined. Plant extracts induced significant enhancement of (26.6-67.7%) of NK cell activity against K562 tumor cells. This increase in NK cell cytotoxicity was found to be due to the enhancement of NK cell production of interferon-gamma (87-337%), and on tumor necrosis factor-alpha (60-200%). Furthermore, the release of both granzyme A and N-acetyl-beta-D-glucosaminidase was increased significantly when compared with controls. Activation of granzyme A and N-acetyl-beta-D-glucosaminidase was clearly observed ranging from 24.2-106.4% to 26.8-110.7%, respectively. Lastly, in the absence of IL-2, plant extracts caused a significant increase in NK-cell-induced cytotoxicity (256%) against K562 tumor cells, and in the presence of IL-2 stimulated cells plant extracts caused an increase in NK cell-cytotoxicity (112%).
Shabsoug
B
B
Department of Chemical Sciences, Jordan University of Science and Technology, Irbid, Jordan. bma1961@yahoo.com
Khalil
R
R
Abuharfeil
N
N
D000118:Acetylglucosaminidase / Q000378:metabolism; D000293:Adolescent; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004306:Dose-Response Relationship, Immunologic; D004789:Enzyme Activation; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D007597:Jordan; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D008297:Male; D009369:Neoplasms / Q000276:immunology*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D055815:Young Adult
D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D000118:Acetylglucosaminidase; D053804:Granzymes