title: Preparation and characterization of poly(l-phenylalanine) chiral stationary phases with varying peptide length.
abstract: Three new chiral stationary phases with different lengths of l-phenylalanine peptide were prepared by solid-phase synthesis with tert-butoxycarbonyl (Boc)-l-phenylalanine on silica. The effect of phenylalanine peptide length on enantioselectivity was studied. The best separation of R/S-warfarin was achieved by the chiral stationary phase with intermediate peptide length. These stationary phases were found to exist mainly in alpha-helical conformation by using FT-IR spectra. The end-capping reagents for the N-terminus of the peptide were also evaluated.
authors.affiliation: Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.forename: Kana
authors.initials: K
authors.lastname: Oyamada
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ohba
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Wada
authors.forename: Toshihide
authors.initials: T
authors.lastname: Maki
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Nakashima
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010455:Peptides / Q000138:chemical synthesis; D010649:Phenylalanine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism; D014859:Warfarin / Q000302:isolation & purification*
chemical_list: D010455:Peptides; D010649:Phenylalanine; D014859:Warfarin; D012822:Silicon Dioxide