Chromosome-scale genetic mapping using a set of 16 conditionally stable Saccharomyces cerevisiae chromosomes.
We have created a resource to rapidly map genetic traits to specific chromosomes in yeast. This mapping is done using a set of 16 yeast strains each containing a different chromosome with a conditionally functional centromere. Conditional centromere function is achieved by integration of a GAL1 promoter in cis to centromere sequences. We show that the 16 yeast chromosomes can be individually lost in diploid strains, which become hemizygous for the destabilized chromosome. Interestingly, most 2n - 1 strains endoduplicate and become 2n. We also demonstrate how chromosome loss in this set of strains can be used to map both recessive and dominant markers to specific chromosomes. In addition, we show that this method can be used to rapidly validate gene assignments from screens of strain libraries such as the yeast gene disruption collection.
Reid
Robert J D
RJ
Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84112, USA.
Sunjevaric
Ivana
I
Voth
Warren P
WP
Ciccone
Samantha
S
Du
Wendy
W
Olsen
Aileen E
AE
Stillman
David J
DJ
Rothstein
Rodney
R
D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics*; D004171:Diploidy; D019656:Loss of Heterozygosity; D008540:Meiosis; D008957:Models, Genetic; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins