title: The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains.
abstract: We isolated the barley stem rust resistance genes Rpg5 and rpg4 by map-based cloning. These genes are colocalized on a 70-kb genomic region that was delimited by recombination. The Rpg5 gene consists of an unusual structure encoding three typical plant disease resistance protein domains: nucleotide-binding site, leucine-rich repeat, and serine threonine protein kinase. The predicted RPG5 protein has two putative transmembrane sites possibly involved in membrane binding. The gene is expressed at low but detectable levels. Posttranscriptional gene silencing using VIGS resulted in a compatible reaction with a normally incompatible stem rust pathogen. Allele sequencing also validated the candidate Rpg5 gene. Allele and recombinant sequencing suggested that the probable rpg4 gene encoded an actin depolymerizing factor-like protein. Involvement of actin depolymerizing factor genes in nonhost resistance has been documented, but discovery of their role in gene-for-gene interaction would be novel and needs to be further substantiated.
authors.affiliation: Department of Crop and Soil Science, Washington State University, Pullman, WA 99164, USA.
authors.forename: R
authors.initials: R
authors.lastname: Brueggeman
authors.forename: A
authors.initials: A
authors.lastname: Druka
authors.forename: J
authors.initials: J
authors.lastname: Nirmala
authors.forename: T
authors.initials: T
authors.lastname: Cavileer
authors.forename: T
authors.initials: T
authors.lastname: Drader
authors.forename: N
authors.initials: N
authors.lastname: Rostoks
authors.forename: A
authors.initials: A
authors.lastname: Mirlohi
authors.forename: H
authors.initials: H
authors.lastname: Bennypaul
authors.forename: U
authors.initials: U
authors.lastname: Gill
authors.forename: D
authors.initials: D
authors.lastname: Kudrna
authors.forename: C
authors.initials: C
authors.lastname: Whitelaw
authors.forename: A
authors.initials: A
authors.lastname: Kilian
authors.forename: F
authors.initials: F
authors.lastname: Han
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.forename: K
authors.initials: K
authors.lastname: Gill
authors.forename: B
authors.initials: B
authors.lastname: Steffenson
authors.forename: A
authors.initials: A
authors.lastname: Kleinhofs
mesh_terms: D001665:Binding Sites; D003001:Cloning, Molecular; D005658:Fungi; D020868:Gene Silencing; D017343:Genes, Plant; D001467:Hordeum / Q000235:genetics* / Q000382:microbiology; D007930:Leucine / Q000737:chemistry; D009711:Nucleotides / Q000378:metabolism; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D018547:Plant Stems / Q000235:genetics / Q000382:microbiology; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D009711:Nucleotides; D010940:Plant Proteins; D011494:Protein Kinases; D007930:Leucine