Multipotency of human neural stem cells from fetal striatum.
We had reported that neural stem cells from human fetal striatum (hsNSCs) expressed neural stem cell markers, and were capable of differentiation into neurons, astrocytes, and oligodendrocytes in vitro. To examine multipotency of hsNSCs, some experiments of transgerm layer differentiation in vitro were carried out. Our data indicated that hsNSCs could also generate osteocytes, adipocytes, and hepatocyte-like cells in vitro. Meanwhile, we injected hsNSCs into murine blastocysts at embryonic day 3.5 of gestation. Microinjection of hsNSCs led to the generation of chimeric embryos. Embryos at embryonic day 3.5 of gestation were shown to contribute to the hsNSC-derived cells by PCR-southern blot of 17alphamod, a special method to discover human cells from animals. Analysis of the donor distribution in different tissues showed that donor-derived cells seeded to various tissues. The cellular nature of the human donor cells in chimeric tissues is, however, currently unknown, and further work will be done to identify what differentiated phenotypes have developed from the human cells.
Li
Yang
Y
Department of Cell Biology, Peking University Health Science Center, Beijing, China. liyang@bjmu.edu.cn
Chi
Xiao-Chun
XC
Li
Xiao-Xia
XX
Xu
Jin-Chong
JC
D017667:Adipocytes / Q000166:cytology; D000818:Animals; D001253:Astrocytes / Q000166:cytology; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D015139:Blotting, Southern; D002454:Cell Differentiation; D002478:Cells, Cultured; D003342:Corpus Striatum; D005260:Female; D005333:Fetus; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008845:Microinjections; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D018008:Myogenin / Q000235:genetics; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D009836:Oligodendroglia / Q000166:cytology; D010011:Osteocytes / Q000166:cytology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D033581:Stem Cell Transplantation / Q000379:methods; D014183:Transplantation, Heterologous
D018008:Myogenin