title: IFATS collection: Immunomodulatory effects of adipose tissue-derived stem cells in an allergic rhinitis mouse model.
abstract: Adipose tissue-derived stem cells (ASCs) exhibit immunosuppressive effects in allogeneic transplantation. However, there is no report that evaluates the in vivo immune-modulating effect of ASCs in an experimental allergic rhinitis (AR) model. We investigated whether ASCs migrate to the nasal mucosa in an AR mouse model and evaluated the immune-modulating effect of ASCs in the AR mouse model. Cultured ASCs (2 x 10(6)) were injected i.v. before the first allergen challenge in the AR mouse model. Migration of ASCs to the nasal mucosa was evaluated by immunofluorescence. The immunomodulatory effects of ASCs were evaluated by nasal symptoms, histology, serum ovalbumin (OVA)-specific antibody, and the cytokine profile of the spleen. ASCs migrated to the nasal mucosa in the AR mouse model. ASCs significantly reduced allergic symptoms and inhibited eosinophilic inflammation in the nasal mucosa. ASCs significantly decreased the serum allergen-specific IgE level and the IgG(1)/IgG(2a) ratio and significantly increased the IgG(2a) level in the AR mouse model. ASCs inhibited interleukin (IL)-4 and IL-5 production from OVA-incubated splenocytes, but enhanced interferon-gamma production. In conclusion, ASCs can migrate to the nasal mucosa in the AR mouse model and inhibit eosinophilic inflammation partly via shifting to a T-helper 1 (Th1) from a Th2 immune response to allergens.
authors.affiliation: Department of Otorhinolaryngology, Medical Research Institute, Pusan National University School of Medicine, Busan, Korea.
authors.forename: Kyu-Sup
authors.initials: KS
authors.lastname: Cho
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Park
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Park
authors.forename: Jin Sup
authors.initials: JS
authors.lastname: Jung
authors.forename: Seong-Gyu
authors.initials: SG
authors.lastname: Jeon
authors.forename: Yoon-Keun
authors.initials: YK
authors.lastname: Kim
authors.forename: Hwan Jung
authors.initials: HJ
authors.lastname: Roh
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D000917:Antibody Formation; D002452:Cell Count; D002454:Cell Differentiation; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D000939:Epitopes; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007155:Immunologic Factors / Q000276:immunology*; D016130:Immunophenotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000276:immunology / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D012221:Rhinitis, Allergic, Perennial / Q000097:blood / Q000276:immunology*; D013154:Spleen / Q000378:metabolism; D013234:Stem Cells / Q000276:immunology*
chemical_list: D016207:Cytokines; D000939:Epitopes; D007074:Immunoglobulin G; D007155:Immunologic Factors; D007073:Immunoglobulin E; D010047:Ovalbumin