Formation and spectroscopy of a tryptophan radical containing peptide in the gas phase.
A doubly deprotonated tryptophan containing peptide was electrosprayed and isolated in an ion trap. UV excitation on this peptide leads to electron detachment and to the formation of an indolyl radical. The photogenerated radical was fragmented by a second laser. The visible spectrum of the gas-phase neutral tryptophan radical containing peptide has been recorded and constitutes a benchmark for calculations and optical measurements.
Joly
Laure
L
Université de Lyon, CNRS, UMR 5579, LASIM, Université Lyon 1, Villeurbanne, F-69622 Lyon, France.
Antoine
Rodolphe
R
Allouche
Abdul-Rahman
AR
Dugourd
Philippe
P
D005609:Free Radicals / Q000737:chemistry / Q000528:radiation effects; D005740:Gases / Q000737:chemistry / Q000528:radiation effects; D008027:Light; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry* / Q000528:radiation effects; D014364:Tryptophan / Q000737:chemistry* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014633:Valine / Q000737:chemistry* / Q000528:radiation effects
D005609:Free Radicals; D005740:Gases; D009842:Oligopeptides; D014364:Tryptophan; D014633:Valine