title: [DNA polymorphism detection of Papaver somniferum L using fluorescent amplified fragment length polymorphism].
abstract: OBJECTIVE
  To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.
  
  
  METHODS
  Genomic DNA was isolated using the AxyPrep DNA Kit, double-digested by two restrictional endonucleases (Eco RI and Mse I) and ligated to oligonucleotide adapters. After Pre-amplification and selective amplification, the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.
  
  
  RESULTS
  More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.
  
  
  CONCLUSIONS
  The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.
authors.affiliation: Institute of Criminal Science and Technology, Yunnan Province Police Bureau, Kunming 650021, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Lu
authors.forename: Bao-wen
authors.initials: BW
authors.lastname: Cheng
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Hong
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Sun
authors.forename: Wen-song
authors.initials: WS
authors.lastname: Zhao
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Yu-rong
authors.initials: YR
authors.lastname: Zhao
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D005456:Fluorescent Dyes; D053803:Forensic Genetics; D010207:Papaver / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D018744:DNA, Plant; D005456:Fluorescent Dyes