title: Contribution of secreted proteases to the pathogenesis of postoperative Enterococcus faecalis endophthalmitis.
abstract: OBJECTIVE
  To determine how a secreted protease contributes to the pathogenesis of post-cataract endophthalmitis caused by Enterococcus faecalis using an aphakic rabbit endophthalmitis model.
  
  
  METHODS
  Department of Ophthalmology, Ehime University School of Medicine, Ehime, Japan.
  
  
  METHODS
  The pathogenesis of E faecalis OG1S (secreted protease-positive) and E faecalis OG1X (secreted protease-negative derivative of OG1S) was compared. After lens removal by phacoemulsification, either strain was inoculated into the lens bag. Changes in bacterial growth, electroretinography (ERG), and pathology of eyes were comparatively monitored throughout the course of the infection. Alternatively, culture fluid from either strain was injected into the vitreous body and ERG and pathology of the eyes were also examined.
  
  
  RESULTS
  The levels of growth in the anterior chamber and vitreous cavity were similar for both strains. However, infection with OG1S resulted in a significantly greater reduction in ERG b-wave amplitude than OG1X. Histological examination showed that the posterior lens capsules were severely affected in eyes infected with OG1S, and inflammatory cells and cocci were found in the anterior vitreous cavity 24 hours after the infection. By 48 hours, the retina architecture was profoundly affected in eyes infected with OG1S. In contrast, few pathological changes were noted in the posterior lens capsules and retina of eyes infected with OG1X. Culture fluid in which OG1S had grown decreased ERG b-wave amplitude and caused morphological changes of the posterior capsule and retina similar to those in the infected eye.
  
  
  CONCLUSIONS
  An extracellular protease plays a major role in the pathogenesis of E faecalis-induced postoperative endophthalmitis.
authors.affiliation: Department of Ophthalmology, Ehime University School of Medicine, Shitsukawa, Toon-shi, Ehime, Japan. takashi.suzuki@schepens.harvard.edu
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Wada
authors.forename: Seiko
authors.initials: S
authors.lastname: Kozai
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Ike
authors.forename: Michael S
authors.initials: MS
authors.lastname: Gilmore
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ohashi
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000382:microbiology / Q000473:pathology; D001426:Bacterial Proteins / Q000502:physiology*; D004195:Disease Models, Animal; D004596:Electroretinography; D009877:Endophthalmitis / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D013293:Enterococcus faecalis / Q000472:pathogenicity*; D015818:Eye Infections, Bacterial / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D005260:Female; D018093:Gelatinases / Q000502:physiology*; D016908:Gram-Positive Bacterial Infections / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D007903:Lens Capsule, Crystalline / Q000382:microbiology / Q000473:pathology; D008297:Male; D018918:Phacoemulsification; D020419:Photoreceptor Cells, Vertebrate / Q000382:microbiology / Q000473:pathology; D011183:Postoperative Complications; D011817:Rabbits; D012697:Serine Endopeptidases / Q000502:physiology*; D014774:Virulence; D014822:Vitreous Body / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D012697:Serine Endopeptidases; D018093:Gelatinases