Plasmid-borne armA methylase gene, together with blaCTX-M-15 and blaTEM-1, in a Klebsiella oxytoca isolate from China.
An armA-producing Klebsiella oxytoca isolate, strain 157, was detected after screening of 447 extended-spectrum beta-lactamase-producing Enterobacteriaceae isolates in China. K. oxytoca 157 was resistant to aminoglycosides, ciprofloxacin and most beta-lactams. Resistance to aminoglycosides and beta-lactams could be transferred to recipient Escherichia coli by conjugation. armA, blaCTX-M-15 and blaTEM-1 genes were detected in K. oxytoca 157 and transconjugant E. coli strain 600(pEC157). Mutation of aa 87 in GyrA was found in K. oxytoca 157. A plasmid of approximately 55 kb was extracted from K. oxytoca 157(pKO157) and E. coli 600(pEC157). Southern blot hybridization confirmed that the armA, blaCTX-M-15 and blaTEM-1 genes were all located on this conjugative plasmid (pEC157). PCR mapping was also performed to investigate the genetic environment of armA. The armA gene was found to be flanked by the same putative transposable elements as reported previously in E. coli, Klebsiella pneumoniae and Citrobacter freundii isolates from different countries.
Zhang
Ying
Y
State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qing Chun Road, Hangzhou, Zhejiang 310003, PR China.
Zhou
Hua
H
State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qing Chun Road, Hangzhou, Zhejiang 310003, PR China.
Shen
Xiao-Qiang
XQ
Department of Respiratory Disease, Hospital of Hangzhou Red Cross Association, No. 208, Huan Cheng Dong Road, Hangzhou, Zhejiang 310006, PR China.
Shen
Ping
P
State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qing Chun Road, Hangzhou, Zhejiang 310003, PR China.
Yu
Yun-Song
YS
State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qing Chun Road, Hangzhou, Zhejiang 310003, PR China.
Li
Lan-Juan
LJ
State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qing Chun Road, Hangzhou, Zhejiang 310003, PR China.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D041121:Klebsiella oxytoca / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S; D008780:Methyltransferases; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1