title: Evaluation of an immunomagnetic separation method to capture Candida yeasts cells in blood.
abstract: BACKGROUND
  Candida species have become the fourth most-frequent cause of nosocomial bloodstream infections in immunocompromised patients. Therefore, rapid identification of pathogenic fungi to species level has been considered critical for treatment. Conventional diagnostic procedures such as blood culture or biochemical tests are lacking both sensitivity and species specificity, so development of rapid diagnostic is essential.
  
  
  RESULTS
  An immunomagnetic method involving anti-Candida monoclonal antibodies was developed to capture and concentrate in human blood four different species of Candida cells responsible for invasive yeast infections. In comparison with an automated blood culture, processing time of immunomagnetic separation is shorter, saving at least 24 hours to obtain colonies before identification.
  
  
  CONCLUSIONS
  Thus, this easy to use method provides a promising basis for concentrating all Candida species in blood to improve sensitivity before identification.
authors.affiliation: Groupe d'Etude des Interactions Hôte-Parasite, UPRES EA 3142, UFR des Sciences Pharmaceutiques et d'Ingénierie de la Santé, Angers Cedex, France. veronique.marchais@univ-angers.fr
authors.forename: Véronique
authors.initials: V
authors.lastname: Apaire-Marchais
authors.forename: Marie
authors.initials: M
authors.lastname: Kempf
authors.forename: Corinne
authors.initials: C
authors.lastname: Lefrançois
authors.forename: Agnès
authors.initials: A
authors.lastname: Marot
authors.forename: Patricia
authors.initials: P
authors.lastname: Licznar
authors.forename: Jane
authors.initials: J
authors.lastname: Cottin
authors.forename: Daniel
authors.initials: D
authors.lastname: Poulain
authors.forename: Raymond
authors.initials: R
authors.lastname: Robert
mesh_terms: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D002175:Candida / Q000254:growth & development / Q000302:isolation & purification*; D002177:Candidiasis / Q000097:blood / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal