Identification of novel p53 target genes by cDNA AFLP in glioblastoma cells.
The p53 plays critical role in cellular functions such as cell cycle arrest and apoptosis. We overexpressed wild-type p53 (wt-p53) in U87 glioblastoma cells via recombinant adenovirus Ad-GFP-P53 which encodes p53 and green fluorescent protein. The transcript profiles were investigated using cDNA amplified fragment length polymorphism approach. Semi-quantitative RT-PCR and DNA sequencing results for the selected genes showed that Cathepsin B and cell cycle associated protein-1 or Caprin-I, genes were suppressed whereas Annexin-II gene overexpressed in response to the overexpression of wt-p53 gene. Our results suggest that these genes could be important mediators of p53-dependent tumor growth suppression in glioblastoma.
Saffari
Mojtaba
M
Department of Medical Genetics, Tehran University of Medical Sciences, Pour Sina Avenue, Tehran, Iran.
Dinehkabodi
Orkideh Saydi
OS
Ghaffari
Seyed Hamid
SH
Modarressi
Mohammad H
MH
Mansouri
Fatemeh
F
Heidari
Mansour
M
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D017306:Annexin A2 / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D002401:Cathepsin B / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D016158:Genes, p53; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017306:Annexin A2; C486562:CAPRIN1 protein, human; D018797:Cell Cycle Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D049452:Green Fluorescent Proteins; D002401:Cathepsin B