Serological and molecular evidence of hepatitis E virus in swine in Brazil.
Active hepatitis E virus (HEV) infections in two Brazilian swine herds were investigated. In study 1, 26 piglets born to five anti-HEV positive sows were monitored from birth to post-partum week 22. Serum samples were screened for the detection of anti-HEV antibodies and a nested RT-PCR used to examine the HEV genome. Passive transfer of immunity was confirmed. At week 22, 23/26 (88.4%) of the piglets had seroconverted. Genome amplification was achieved in a feces pool from one holding pen and in one serum sample, both from 13-week-old animals. Histology was suggestive of a potential HEV infection. In the second study, 47 piglets born to six anti-HEV-positive sows were monitored after weaning. Seroconversion was determined in eight animals at 6-8 weeks of age. HEV RNA was detected in two pools from a holding pen for 12-16-week-old animals. Brazilian isolates were classified as genotype 3. This is the first molecular evidence of HEV infection in Brazilian pig herds.
dos Santos
Debora Regina Lopes
DR
Laboratório de Desenvolvimento Tecnológico, Instituto Oswaldo Cruz-Fiocruz, Avenida Brasil 4365, Pavilhão Helio e Peggy Pereira, 2 degrees andar, 21040-900 Rio de Janeiro, RJ, Brazil. santosdl@ioc.fiocruz.br
Vitral
Claudia Lamarca
CL
de Paula
Vanessa Salete
VS
Marchevsky
Renato Sergio
RS
Lopes
Juliana Freitas
JF
Gaspar
Ana Maria Coimbra
AM
Saddi
Telma Michella
TM
Júnior
Nilton Cecílio de Mesquita
NC
Guimarães
Flavio de Rezende
Fde R
Júnior
João Garcia Caramori
JG
Ximenes
Lia Laura Lewis
LL
Souto
Francisco José Dutra
FJ
Pinto
Marcelo Alves
MA
D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood*; D001938:Brazil / Q000453:epidemiology; D004197:Disease Reservoirs / Q000662:veterinary / Q000821:virology; D005260:Female; D005784:Gene Amplification; D005838:Genotype; D016751:Hepatitis E / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D011247:Pregnancy; D011634:Public Health; D012367:RNA, Viral / Q000032:analysis*; D012189:Retrospective Studies; D016036:Seroepidemiologic Studies; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000635:transmission / Q000821:virology; D015047:Zoonoses
D000914:Antibodies, Viral; D012367:RNA, Viral