T6092C polymorphism of SLC22A12 gene is associated with serum uric acid concentrations in Korean male subjects.
OBJECTIVE
It has been suggested that the SLC22A12 gene polymorphism may be involved in the mechanism of renal urate handling. The purpose of the current study was to identify the effect of the T6092C polymorphism of the SLC22A12 gene on serum uric acid concentrations in the Korean population.


METHODS
We examined 196 healthy subjects (141 males and 55 females) in this study. Among the SLC22A12 gene polymorphism, the T6092C polymorphism in intron 4 at rs1529909 of the SLC22A12 gene was evaluated using denaturing high performance liquid chromatography analysis. Diverse clinical parameters of renal urate handling derived from fasting blood and urine samples, such as the serum uric acid concentration and the fractional excretion of uric acid (FEUA), were also assessed for identification of the relationship between genotypic variations. Statistical analysis was performed using the chi-square test, ANOVA test, and the Pearson correlation coefficient analysis to determine which indicators involved serum uric acid. And the significance of these indicators was then confirmed by multivariate regression analysis.


RESULTS
The prevalence of the T6092C polymorphism (TT, TC, and CC) was 58.2%, 37.2%, and 4.6%, respectively. Only the concentration of serum uric acid and the FEUA in male subjects differed significantly among each genotype (p=0.038 and p=0.013, respectively). Serum uric acid concentrations in male subjects with the TT genotype were increased compared with those with the TC as well as the TC or CC genotypes (6.2+/-1.2 vs. 5.7+/-1.3, p=0.039 and 6.2+/-1.2 vs. 5.6+/-1.3, p=0.019, respectively), whereas there was no significant difference between CC genotype and TT genotype in serum uric acid (p=0.066). No significant difference between clinical indicators and genotypes existed in females. The T6092C polymorphism of the SLC22A12 gene, serum creatinine, and the FEUA were significantly associated with the concentration of serum uric acid.


CONCLUSIONS
This study showed that the T6092C polymorphism of the SLC22A12 gene may be involved in renal urate handling and the concentration of serum uric acid, in male subjects.
Jang
Won Cheoul
WC
Department of Chemistry, School of Advanced Science, Dankook University, Cheonan, South Korea.
Nam
Yun-Hyoung
YH
Park
Su-Min
SM
Ahn
Young-Chang
YC
Park
Sung-Hoon
SH
Choe
Jung-Yoon
JY
Shin
Im-Hee
IH
Kim
Seong-Kyu
SK
D000328:Adult; D000704:Analysis of Variance; D000886:Anthropometry; D001794:Blood Pressure / Q000502:physiology; D015992:Body Mass Index; D002851:Chromatography, High Pressure Liquid; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008297:Male; D027361:Organic Anion Transporters / Q000235:genetics*; D027701:Organic Cation Transport Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D012015:Reference Standards; D012044:Regression Analysis; D014527:Uric Acid / Q000097:blood* / Q000652:urine
D027361:Organic Anion Transporters; D027701:Organic Cation Transport Proteins; C491241:SLC22A12 protein, human; D014527:Uric Acid