The marker SCK13(603) associated with resistance to ascochyta blight in chickpea is located in a region of a putative retrotransposon.
The sequence characterized amplified region (SCAR) marker SCK13(603), associated with ascochyta blight resistance in a chickpea recombinant inbred line (RIL) population, was used as anchored sequence for genome walking. The PCRs performed in the walking steps to walk in the same direction produced eight bands in 5' direction and five bands in 3' direction with a length ranking from 530 to 2,871 bp. The assembly of the bands sequences along with the sequence of SCK13(603) resulted in 7,815 bp contig. Blastn analyses showed stretches of DNA sequence mainly distributed from the nucleotides 1,500 to 4,500 significantly similar to Medicago truncatula genomic DNA. Three open reading frames (ORFs) were identified and blastp analysis of predicted amino acids sequences revealed that ORF1, ORF2 and ORF3 had significant similarity to a CCHC zinc finger protein, to an integrase, and to a precursor of the glucoamylase s1/s2, respectively, from M. truncatula. The high homology of the putative proteins derived from ORF1 and ORF2 with retrotransposon proteins and the prediction of the existence of conserved domains usually present in retrotransposon proteins indicate that the marker SCK13(603) is located in a region of a putative retrotransposon. The information generated in this study has contributed to increase the knowledge of this important region for blight resistance in chickpea.
Iruela
Marta
M
Dpto. Mejora Genética Vegetal, IAS-CSIC, Córdoba, 14080, Córdoba, Spain.
Pistón
Fernando
F
Cubero
José Ignacio
JI
Millán
Teresa
T
Barro
Francisco
F
Gil
Juan
J
D000595:Amino Acid Sequence; D001203:Ascomycota; D016386:Chromosome Walking; D029863:Cicer / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D018626:Retroelements; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D005819:Genetic Markers; D018626:Retroelements