Intratubular transplantation as a strategy for establishing animal models of testicular germ cell tumours.
Testicular germ cell tumours (TGCTs) are prevalent cancers among young men. Currently, there is no reliable animal model for TGCTs. To establish such animal models, we have explored the possibility of intratubular testicular transplantation as means to deliver tumour cells into the seminiferous tubules of host animals. Our results demonstrated that transplanted cells could effectively populate the testis of a recipient mouse and develop into TGCTs. In addition, the donor cells could be transfected with a specific transgene before transplantation, thereby providing an approach to evaluate the specific effects of gene functions in the oncogenic processes. Hence, depending on selection of specific donor cells or mixtures of donor cells, transplantation models of TGCTs could be significant for studies on the pathogenesis, diagnosis and therapies of such a prevalent and important cancer in men.
Li
Yunmin
Y
Division of Cell and Developmental Genetics, Department of Medicine, University of California, San Francisco, San Francisco, CA 94121, USA.
Kido
Tatsuo
T
Luo
Jinping
J
Fukuda
Michiko
M
Dobrinski
Ina
I
Lau
Yun-Fai Chris
YF
D000328:Adult; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D023421:Models, Animal; D009368:Neoplasm Transplantation / Q000379:methods*; D012671:Seminiferous Tubules; D018239:Seminoma; D013736:Testicular Neoplasms
D014408:Biomarkers, Tumor