DNA sequencing-based typing of HPA-1 to HPA-17w systems.
Although several DNA-based human platelet antigens (HPA) typing techniques, such as PCR-SSP and PCR-SSO, have been established, the typing errors and the lack of interlaboratory reproducibility are still the issues of concerns. In the present study, polymerase chain reaction primers were designed for identification of all the phenotypically different HPA-1 to HPA-17w types by sequencing-based typing (SBT) method using genomic DNA samples. No discrepancies were observed between PCR-SSP typing and SBT typing in typing a panel of HPA-typed platelet donors that included all common HPA types and the rare HPA-1b, 2b, 3b, and 6bw homozygous donors.
Wu
Guo-Guang
GG
Nan-Ning Institute of Transfusion Medicine, 18 Keyuan Avenue, 530003, Nanning, China. guangwu@szonline.net.
Tang
Qiu-Ming
QM
Nan-Ning Institute of Transfusion Medicine, 18 Keyuan Avenue, 530003, Nanning, China.
Shen
Wei-Dong
WD
Nan-Ning Institute of Transfusion Medicine, 18 Keyuan Avenue, 530003, Nanning, China.
Liao
Yan
Y
Nan-Ning Institute of Transfusion Medicine, 18 Keyuan Avenue, 530003, Nanning, China.
Li
Heng-Cong
HC
Nan-Ning Institute of Transfusion Medicine, 18 Keyuan Avenue, 530003, Nanning, China.
Zhao
Tong-Mao
TM
National Institutes of Health, Bethesda, USA.
D016824:Antigens, Human Platelet / Q000235:genetics*; D005260:Female; D006801:Humans; D019520:Living Donors; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
D016824:Antigens, Human Platelet