title: Alumina sol-gel/sonogel-carbon electrode based on acetylcholinesterase for detection of organophosphorus pesticides.
abstract: Two new amperometric biosensors based on immobilization of acetylcholinesterase on a sonogel-carbon electrode for detection of organophosphorous compounds are proposed. The electrodes were prepared applying high-energy ultrasounds directly to the precursors. The first biosensor was obtained by simple entrapping acetylcholinesterase in Al(2)O(3) sol-gel matrix on the sonogel-carbon. The second biosensor was produced in a sandwich configuration. Its preparation involved adsorption of the enzyme and modification via a polymeric membrane such as polyethylene glycol and the ion-exchanger Nafion. The optimal enzyme loading was found to be 0.7 mIU. Both biosensors showed optimal activity in 0.2 M phosphate buffer, pH 7.0, at an operating potential of 210 mV. The detection limit achieved for chlorpyriphos-ethyl-oxon was 2.5x10(-10)M at a 10-min incubation time.
authors.affiliation: Centre de phytopharmacie, Université de Perpignan-Biomem, Perpignan Cedex, France.
authors.forename: Hanane
authors.initials: H
authors.lastname: Zejli
authors.forename: José Luis
authors.initials: JL
authors.lastname: Hidalgo-Hidalgo de Cisneros
authors.forename: Ignacio
authors.initials: I
authors.lastname: Naranjo-Rodriguez
authors.forename: Baohong
authors.initials: B
authors.lastname: Liu
authors.forename: Khalid Riffi
authors.initials: KR
authors.lastname: Temsamani
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Marty
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000537:Aluminum Oxide / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000737:chemistry; D010575:Pesticides / Q000032:analysis* / Q000737:chemistry*; D044367:Phase Transition; D015203:Reproducibility of Results
chemical_list: D009943:Organophosphorus Compounds; D010575:Pesticides; D002244:Carbon; D000110:Acetylcholinesterase; D000537:Aluminum Oxide