title: Genetic analysis of two porcine reproductive and respiratory syndrome viruses with different virulence isolated in China.
abstract: The S1 and SY0608 strains of porcine reproductive and respiratory syndrome virus (PRRSV) were individually isolated and had different pathogenicity in pigs in 1997 and 2006. In order to understand their genomic characteristics, the full-length genome of S1 and SY0608 isolates were sequenced and analyzed. The results indicated that their genome composition differed significantly and shared only 88.5% nucleotide identity with each other. The genetic variation and amino acid substitutions were not randomly distributed in the genome, and mainly focused on ORF1a, ORF3 and ORF5. The SY0608 strain, with high pathogenicity, had a 30-amino-acid deletion at amino acid positions 480 and 532-560 in comparison with the S1 strain. The alignment of amino acid sequence of Nsp1-Nsp8, GP2-GP5, M and N of S1 and SY0608 with other PRRSV isolates demonstrated that variation was mainly found in the Nsp2, GP3 and GP5 proteins. In comparison with the S1 strain, the SY0608 strain showed some potential glycosylation site mutations in GP5 at amino acid positions between 26 and 39, which might be associated with viral antigenicity. Phylogenetic analysis showed that the two strains belonged to two different branches that do not indicate differences in pathogenicity. Interestingly, the deletion strains isolated recently in China formed a new minor branch, revealing the same evolutionary trend.
authors.affiliation: Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.forename: Xinglong
authors.initials: X
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.forename: Xianwei
authors.initials: X
authors.lastname: Wang
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D002681:China; D016000:Cluster Analysis; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D012367:RNA, Viral / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics; D014774:Virulence
chemical_list: D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins