Structural and functional analysis of SGT1-HSP90 core complex required for innate immunity in plants.
SGT1 (Suppressor of G2 allele of skp1), a co-chaperone of HSP90 (Heat-shock protein 90), is required for innate immunity in plants and animals. Unveiling the cross talks between SGT1 and other co-chaperones such as p23, AHA1 (Activator of HSP90 ATPase 1) or RAR1 (Required for Mla12 resistance) is an important step towards understanding the HSP90 machinery. Nuclear magnetic resonance spectroscopy and mutational analyses of HSP90 revealed the nature of its binding with the CS domain of SGT1. Although CS is structurally similar to p23, these domains were found to non-competitively bind to various regions of HSP90; yet, unexpectedly, full-length SGT1 could displace p23 from HSP90. RAR1 partly shares the same binding site with HSP90 as the CS domain, whereas AHA1 does not. This analysis allowed us to build a structural model of the HSP90-SGT1 complex and to obtain a compensatory mutant pair between both partners that is able to restore virus resistance in vivo through Rx (Resistance to potato virus X) immune sensor stabilization.
Kadota
Yasuhiro
Y
RIKEN Plant Science Center, Suehiro-cho 1-7-22 Tsurumi-ku, Yokohama, Japan.
Amigues
Beatrice
B
Ducassou
Lionel
L
Madaoui
Hocine
H
Ochsenbein
Francoise
F
Guerois
Raphaël
R
Shirasu
Ken
K
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D005964:Glucosyltransferases / Q000737:chemistry* / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism*; D007113:Immunity, Innate / Q000276:immunology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010935:Plant Diseases / Q000276:immunology; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship; D014908:Triticum
D029681:Arabidopsis Proteins; D018841:HSP90 Heat-Shock Proteins; D005964:Glucosyltransferases; C515668:SGT1 protein, Arabidopsis