title: Imaging individual mRNA molecules using multiple singly labeled probes.
abstract: We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons.
authors.affiliation: Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. arjunraj@mit.edu
authors.forename: Arjun
authors.initials: A
authors.lastname: Raj
authors.forename: Patrick
authors.initials: P
authors.lastname: van den Bogaard
authors.forename: Scott A
authors.initials: SA
authors.lastname: Rifkin
authors.forename: Alexander
authors.initials: A
authors.lastname: van Oudenaarden
authors.forename: Sanjay
authors.initials: S
authors.lastname: Tyagi
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008322:Mammals; D015335:Molecular Probes; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics
chemical_list: D015335:Molecular Probes; D012333:RNA, Messenger