[Screening of anti-tumor parts from the seeds of Livistona chinensis and its anti-angiogenesis effect].
OBJECTIVE
To screen the anti-tumor active parts from the seeds of Livistona chinensis R. Br. and its mechanism of anti-angiogenesis effect.


METHODS
MTT assay and ELISA were used to detect the proliferation and the secretion of vascular endothelial growth factor (VEGF) of chronic myelogenous leukemia K562, ovarian neoplasm SKOV3, colon carcinoma HT-29 and bladder cancer T24 cell lines respectively. The expression of fetal liver kinase (Flk-1) protein and mRNA in endothelial cells induced by VEGF was analyzed by Western blotting and RT-PCR.


RESULTS
The ethyl acetate part of alcohol extract from seeds of Livistona chinensis R. Br. could selectively inhibit the proliferation of HT-29 and T24 cell lines in a dose-dependent manner, the maximum inhibitory rate were 74.66% and 86.52%, and could significantly inhibit the secretion of VEGF protein of the four tumor cell lines. The petroleum ether part had similar inhibitory effect as well as the ethyl acetate part, but its potency was inferior to the ethyl acetate part, the maximum inhibitory rate were 43.80% and 38.67%, the water-soluble and n-butanol parts had no effect on different tumor cell lines. The ethyl acetate part could reduce significantly the expression of Flk-1 protein and mRNA of HUVEC induced by VEGF.


CONCLUSIONS
The ethyl acetate part, which is considered as active part of alcohol extract from seeds of Livistona chinensis R. Br., has fine anti-tumor activity, and its mechanism might be associated with reducing VEGF protein secretion and inhibiting the expression of Flk-1 mRNA and protein.
Wang
Hui
H
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Li
Ao
A
Dong
Xiao-Ping
XP
Xu
Xiao-Yu
XY
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D028023:Arecaceae / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol; D019073:HT29 Cells; D006801:Humans; D020014:K562 Cells; D009389:Neovascularization, Pathologic; D010946:Plants, Medicinal / Q000737:chemistry; D011244:Pregnadienediols; D012333:RNA, Messenger / Q000378:metabolism; D040262:Receptors, Vascular Endothelial Growth Factor / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D020533:Angiogenesis Inhibitors; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D011244:Pregnadienediols; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D000431:Ethanol; D040262:Receptors, Vascular Endothelial Growth Factor; C418047:anecortave acetate