Production, partial purification and characterization of feruloyl esterase by Aspergillus awamori in submerged fermentation.
Microbial feruloyl esterases acting on plant cell wall polymers represent key tools for the degradation of plant cell wall. In this paper, we describe in detail the microbial production, partial purification and characterization of feruloyl esterase from a culture medium of Aspergillus awamori strain IFO4033 obtained from a crude hemicellulose preparation of wheat straw, corncobs and wheat germ. Feruloyl esterase was extracted using centrifugation and dialysis, and then purified by ion exchange chromatography and microfiltration to homogeneity, which was checked by SDSPAGE and isoelectric focusing-PAGE. Protein content and activity of the enzyme were measured in each step of extraction and purification. Biomass was determined by the dry weight method. pH and temperature optima of feruloyl esterase enzyme were also determined. The effects of culturing time, and carbon and nitrogen sources on enzyme production were systematically investigated. Finally, enzyme activities under different storage conditions were examined.
Fazary
Ahmed Eid
AE
Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
Ju
Yi-Hsu
YH
D001230:Aspergillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009584:Nitrogen / Q000494:pharmacology
D005656:Fungal Proteins; D002244:Carbon; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D009584:Nitrogen