title: Application of liquid-based preparation to fine needle aspiration cytology in breast cancer.
abstract: OBJECTIVE
  To examine the performance of liquid-based cytology (LBC) in breast cytology to confirm the diagnosis of carcinoma.
  
  
  METHODS
  Using cell clusters directly scratched from surgically removed tumor masses, we examined the immunocytochemistry, molecular biology and cytomorphology of the specimens.
  
  
  RESULTS
  LBC was very useful for gene analysis and evaluating the immunocytochemistry. The cytologic features of LBC were slightly different from those ofa conventional aspiration cytology smear.
  
  
  CONCLUSIONS
  LBC is a promising method for improving the standardization ofpreparations in breast cytology, although care should be taken to account for its characteristic cytologic features. The quantitative analysis of HER-2 mRNA correlated with the results of immunohistochemistry.
authors.affiliation: Pathology Laboratory, Nihon University Itabashi Hospital, Tokyo, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Komatsu
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Toshimi
authors.initials: T
authors.lastname: Seki
authors.forename: Atsuko
authors.initials: A
authors.lastname: Yoshino
authors.forename: Fumi
authors.initials: F
authors.lastname: Fuchinoue
authors.forename: Sadao
authors.initials: S
authors.lastname: Amano
authors.forename: Akio
authors.initials: A
authors.lastname: Komatsu
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sugitani
authors.forename: Norimichi
authors.initials: N
authors.lastname: Nemoto
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D044963:Biopsy, Fine-Needle / Q000379:methods; D001943:Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D003581:Cytodiagnosis / Q000379:methods; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human