Hepatic iron content corresponds with the susceptibility of lymphocytes to oxidative stress in neonatal pigs.
The pig is born with limited iron supplies. If not supplemented, piglets dramatically loose their body iron stores during the first few days of postnatal life. The aim of this study was to investigate the influence of hepatic iron content on susceptibility of blood cells to oxidative stress. Four 1-day-old and three 7-days-old animals were used in this study. The alkaline version of the comet assay was used to measure DNA damage. As expected, iron body stores of non-supplemented animals decrease significantly during the first 4 days of life. However, no difference in background DNA damage was found between untreated lymphocytes from these two groups of animals, despite the difference in their hepatic iron content. Interestingly, DNA damage induced by H2O2 and X-radiation in lymphocytes taken from 1-day-old piglets was significantly higher than in those taken from 7-days-old animals. In contrast, NaOCl or tert-butyl-hydroxide also induced significant amounts of DNA damage, but no differences between the two groups of piglets were found. Our data show that decreased hepatic iron content corresponds with decreased susceptibility of blood lymphocytes to oxidative stressors.
Kruszewski
Marcin
M
Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, ul. Dorodna 16, 03-195 Warszawa, Poland. marcinkr@orange.ichtj.waw.pl
Iwaneńko
Teresa
T
Bartłomiejczyk
Teresa
T
Woliński
Jarosław
J
Starzyński
Rafał R
RR
Gralak
Mikołaj A
MA
Zabielski
Romuald
R
Lipiński
Paweł
P
D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000097:blood / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress; D013552:Swine; D014965:X-Rays / Q000009:adverse effects; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D007501:Iron