title: [Protective activity of secreted proteins of Streptococcus pneumoniae and Klebsiella pneumoniae].
abstract: Protective efficacy of secreted proteins of Streptococcus pneumoniae and Klebsiella pneumoniae cultivated on cardiocerebral broth and semisynthetic growth medium respectively was studied in vivo. Fraction with molecular weight 30 - 50 kDa obtained by the method of membrane fractionation had high protective efficacy. Two-dose immunization of mice with this fraction provided 80 - 100% protection from infection by homologous strains of S. pneumoniae and K. pneumoniae. Cross-protective activity of the fraction was revealed when infecting immunized mice by different K-types of K. pneumoniae. Blood sera of mice immunized with 30 - 50 kDa fraction possessed preventive features protecting from infection 90% of animals while 100% of death in the control group. It was determined that protective efficacy of the mentioned fraction was determined by protein-containing antigens because proteolytic disruption of the protein component resulted in loss of protective properties of the preparation.
authors.forename: A V
authors.initials: AV
authors.lastname: Trishin
authors.forename: F V
authors.initials: FV
authors.lastname: Donenko
authors.forename: E A
authors.initials: EA
authors.lastname: Kurbatova
authors.forename: K E
authors.initials: KE
authors.lastname: Voiushin
authors.forename: M V
authors.initials: MV
authors.lastname: Kiselevskiĭ
authors.forename: N B
authors.initials: NB
authors.lastname: Egorova
authors.forename: I M
authors.initials: IM
authors.lastname: Gruber
authors.forename: I B
authors.initials: IB
authors.lastname: Semenova
authors.forename: B F
authors.initials: BF
authors.lastname: Semenov
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D003470:Culture Media; D007106:Immune Sera / Q000008:administration & dosage; D007116:Immunization, Passive; D007710:Klebsiella Infections / Q000517:prevention & control*; D007711:Klebsiella pneumoniae / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D011008:Pneumococcal Infections / Q000517:prevention & control*; D013296:Streptococcus pneumoniae / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D007106:Immune Sera