title: Cerebrospinal fluid phospholipid changes following traumatic brain injury.
abstract: Traumatic brain injury (TBI) is a leading cause of morbidity and mortality, with approximately 1.4 million people suffering a TBI each year. With TBI, a cascade of events is initiated including the activation of phospholipases, which leads to the disruption of the lipid bilayer of the membrane of neurons and neuroglia. The purpose of this study is to describe phospholipid changes following TBI. A total of 39 cerebrospinal fluid samples were obtained from the ventricular catheter system of 10 participants who received a TBI as a result of a motor vehicle crash, being struck by a vehicle as a pedestrian, or a fall. Phospholipids were extracted from samples and measured by normal-phase high-performance liquid chromatography with ultraviolet detector at a wavelength of 206 nm. The highest mean concentration of lysophosphatidylcholine occurred on Day 1 after injury. The concentration of phosphatidylserine was variable, with the highest mean concentration occurring on Day 2 after injury. The highest mean concentrations of phosphatidylethanolamine, phosphatidylcholine, and sphingomyelin occurred on Day 4 after injury. Findings provide preliminary evidence for disruption of central nervous system membrane phospholipids following TBI.
authors.affiliation: College of Nursing, The University of Arizona, Tucson, Arizona 85721, USA. pasvogel@nursing.arizona.edu
authors.forename: Alice E
authors.initials: AE
authors.lastname: Pasvogel
authors.forename: Petra
authors.initials: P
authors.lastname: Miketova
authors.forename: Ida M Ki
authors.initials: IM
authors.lastname: Moore
mesh_terms: D001930:Brain Injuries / Q000134:cerebrospinal fluid*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008137:Longitudinal Studies; D010743:Phospholipids / Q000134:cerebrospinal fluid*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010743:Phospholipids