Phenotypic evaluation of the effect of anaerobiosis on some virulence attributes of Candida albicans.
The current assumption that Candida albicans is a facultatively anaerobic organism has been widely accepted since its recovery from anoxic sites became common. However, the link between anaerobiosis and virulence remains uncertain. This study investigated the differential cell-surface hydrophobicity (CSH) using a hydrocarbon/water partition technique and analysed the differential secretion rates of secretory aspartyl proteases (Saps), esterase, chondroitinase and haemolysins of C. albicans strains recovered from periodontal pockets and non-periodontium-related intra-oral sites. For the enzymic tests, all strains from both sets were grown under aerobic and anaerobic conditions and the harvested cells were inoculated onto suitable normal or pre-reduced culture media in the presence or absence of molecular oxygen, respectively. The results showed that no variations were perceptible for CSH and chondroitinase (P>0.05). The secretion rates of esterase and haemolysins strongly decreased in an anoxic environment (P<0.0001). However, a consistent increment (P<0.0001) in Sap secretion was detected when cultures were grown under anaerobic conditions. Based on these results, it is suggested that the oxygen concentration in the atmosphere surrounding cells exerts a variable influence on the virulence attributes of C. albicans.
Rosa
Edvaldo Antonio Ribeiro
EAR
Oral Biosciences Unit, Faculty of Dentistry, Prince Philip Dental Hospital, University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR.|Laboratory of Stomatology, Faculty of Dentistry, Biological and Health Sciences Centre, Pontifical Catholic University of Paraná, Rua Imaculada Conceição 1155, Curitiba PR 80215-901, Brazil.
Rached
Rodrigo Nunes
RN
Laboratory of Stomatology, Faculty of Dentistry, Biological and Health Sciences Centre, Pontifical Catholic University of Paraná, Rua Imaculada Conceição 1155, Curitiba PR 80215-901, Brazil.
Ignácio
Sérgio Aparecido
SA
Laboratory of Stomatology, Faculty of Dentistry, Biological and Health Sciences Centre, Pontifical Catholic University of Paraná, Rua Imaculada Conceição 1155, Curitiba PR 80215-901, Brazil.
Rosa
Rosimeire Takaki
RT
Laboratory of Stomatology, Faculty of Dentistry, Biological and Health Sciences Centre, Pontifical Catholic University of Paraná, Rua Imaculada Conceição 1155, Curitiba PR 80215-901, Brazil.
José da Silva
Wander
W
Department of Prosthetic Dentistry, Faculty of Dentistry of Piracicaba, State University of Campinas, Avenida Limeira 901, Piracicaba SP 13414-903, Brazil.|Oral Biosciences Unit, Faculty of Dentistry, Prince Philip Dental Hospital, University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR.
Yau
Joyce Yick Yee
JYY
Oral Biosciences Unit, Faculty of Dentistry, Prince Philip Dental Hospital, University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR.
Samaranayake
Lakshman Perera
LP
Oral Biosciences Unit, Faculty of Dentistry, Prince Philip Dental Hospital, University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR.
D000693:Anaerobiosis / Q000502:physiology; D002176:Candida albicans / Q000378:metabolism* / Q000472:pathogenicity*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D006801:Humans; D010514:Periodontal Pocket / Q000382:microbiology; D010641:Phenotype; D014774:Virulence
D005656:Fungal Proteins