The mechanism of acidic hydrolysis of esters explains the HDV ribozyme activity.
The hepatitis delta virus (HDV) ribozyme is an RNA enzyme that catalyzes the site-specific trans-esterification reaction. Using high hydrostatic pressure (HHP) technique we showed that HDV ribozyme catalyzes the reaction of RNA cleavage in the absence of magnesium ions according to mechanism of acidic hydrolysis of esters. HHP induces changes of water structure, lowering pH and effect ribozyme catalytic site structure formation without magnesium. HHP, similarly to magnesium ion at ambient pressure stabilizes the higher order RNA structure of HDV, but Mg(2+) is not involved in the catalysis. Our results clearly support the new mechanism of HDV hydrolysis and show advantages of using HHP in analysis of macromolecules interaction.
Fedoruk-Wyszomirska
Agnieszka
A
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, Poznan, Poland.
Giel-Pietraszuk
Małgorzata
M
Wyszko
Eliza
E
Szymański
Maciej
M
Ciesiołka
Jerzy
J
Barciszewska
Mirosława Z
MZ
Barciszewski
Jan
J
D000143:Acids / Q000378:metabolism*; D001483:Base Sequence; D055162:Biocatalysis; D004952:Esters / Q000378:metabolism*; D016679:Genome, Viral / Q000235:genetics; D003698:Hepatitis Delta Virus / Q000201:enzymology*; D006868:Hydrolysis; D006874:Hydrostatic Pressure; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D000143:Acids; D004952:Esters; D016337:RNA, Catalytic