Protein kinase C regulates the expression of M1 receptors and BDNF in rat retinal cells.
Protein kinase C (PKC) plays a key role in cellular events including proliferation, survival and differentiation. Our previous study showed the effect of phorbol 12-myristate 13-acetate (PMA), a PKC activator, inducing a decrease in retinal cells proliferation. This effect was mediated by muscarinic type 1 receptors (M1) activation and brain derived neurotrophic factor (BDNF) treatment also induced a decrease in cell proliferation. Based on these results we analyzed the expression of either M1 receptors or BDNF following PMA treatment of retinal cell cultures. Our data demonstrated that PMA induced a decrease in both protein expressions after 48 h in culture. However, after 45 min, PMA induced a transient increase in BDNF expression and a decrease in M1 receptors expression. Analyzing the expression of M1 receptors and BDNF during the postnatal development in vivo, we observed a decrease in both proteins. Taken together our results suggest the involvement of PKC in the control of M1 expression in retinal cells.
dos Santos
Aline Araujo
AA
Departamento de Neurobiologia, Programa de Neuroimunologia, Instituto de Biologia, Centro de Estudos Gerais, Universidade Federal Fluminense, Niterói, Rio de Janeiro,Brazil.
Medina
Simone Vidal
SV
de Araújo Martins
Leandro
L
de Araujo
Elizabeth Giestal
EG
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D043643:Receptor, Muscarinic M1 / Q000096:biosynthesis*; D012160:Retina / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D019208:Brain-Derived Neurotrophic Factor; D043643:Receptor, Muscarinic M1; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate