Two homologous Agr-like quorum-sensing systems cooperatively control adherence, cell morphology, and cell viability properties in Lactobacillus plantarum WCFS1.
A two-component regulatory system of Lactobacillus plantarum, encoded by genes designated lamK and lamR (hpk10 and rrp10), was studied. The lamK and lamR genes encode proteins which are highly homologous to the quorum-sensing histidine kinase LamC and the response regulator LamA, respectively. Transcription analysis of the lamKR operon and the lamBDCA operon and liquid chromatography-mass spectrometry analysis of production of the LamD558 autoinducing peptide were performed for DeltalamA, DeltalamR, DeltalamA DeltalamR deletion mutants and a wild-type strain. The results suggested that lamA and lamR are cooperating genes. In addition, typical phenotypes of the DeltalamA mutant, such as reduced adherence to glass surfaces and filamentous cell morphology, were enhanced in the DeltalamA DeltalamR mutant. Microarray analysis suggested that the same cell wall polysaccharide synthesis genes, stress response-related genes, and cell wall protein-encoding genes were affected in the DeltalamA and DeltalamA DeltalamR mutants. However, the regulation ratio was more significant for the DeltalamA DeltalamR mutant, indicating the cooperative effect of LamA and LamR.
Fujii
Toshio
T
Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands. tfujii@kirin.co.jp
Ingham
Colin
C
Nakayama
Jiro
J
Beerthuyzen
Marke
M
Kunuki
Ryoko
R
Molenaar
Douwe
D
Sturme
Mark
M
Vaughan
Elaine
E
Kleerebezem
Michiel
M
de Vos
Willem
W
D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002470:Cell Survival; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D048191:Lactiplantibacillus plantarum / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D053038:Quorum Sensing / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
D001426:Bacterial Proteins; D015534:Trans-Activators