title: Gluconobacter sphaericus (Ameyama 1975) comb. nov., a brown pigment-producing acetic acid bacterium in the Alphaproteobacteria.
abstract: Strain NBRC 12467T was examined genetically, phylogenetically, phenotypically, and chemotaxonomically. The DNA G+C content of the strain was 59.5 mol%. The strain represented low levels of DNA-DNA hybridization of 49-9% to the type strains of eight Gluconobacter species. The strain formed a cluster along with the type strains of G. albidus and G. kondonii in phylogenetic trees based on 16S rRNA gene sequences. In a phylogenetic tree based on 16S-23S rRNA gene ITS sequences, however, the strain formed an independent cluster from the type strains of the eight Gluconobacter species. Such phylogenetic relationships were supported by the calculated pair-wise 16S rRNA gene and 16S-23S rRNA gene ITS sequence similarities. The strain was distinguished from the type strains of the eight Gluconobacter species by 16S-23S rRNA gene ITS restriction analysis using five restriction endonucleases. The strain produced a water-soluble brown pigment and 2,5-diketo-D-gluconate from D-glucose, differing from the type strains of the eight Gluconobacter species, and acid from meso-erythritol very weakly, differing from the type strains of the remaining seven Gluconobacter species except for the type strain of G. roseus, but not from maltose, differing from the type strain of G. oxydans, and had Q-10. For the strain, which was once classified as G. oxydans subsp. sphaericus, Gluconobacter sphaericus (Ameyama 1975) comb. nov. is proposed. The type strain is NBRC 12467T, which is also deposited as BCC 14448T.
authors.affiliation: BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand.
authors.forename: Taweesak
authors.initials: T
authors.lastname: Malimas
authors.forename: Pattaraporn
authors.initials: P
authors.lastname: Yukphan
authors.forename: Mai
authors.initials: M
authors.lastname: Takahashi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Muramatsu
authors.forename: Mika
authors.initials: M
authors.lastname: Kaneyasu
authors.forename: Wanchern
authors.initials: W
authors.lastname: Potacharoen
authors.forename: Somboon
authors.initials: S
authors.lastname: Tanasupawat
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Morakot
authors.initials: M
authors.lastname: Tanticharoen
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Yamada
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D005798:Genes, Bacterial; D020459:Genes, rRNA; D020576:Gluconobacter / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S