Scrophularia buergeriana regulates cytokine production in vitro.
The Scrophularia buergeriana (SB) has long been used to treat various diseases an account of its antimicrobial and anti-virus activity. However, it is unclear how SB regulates the immune responses. This study investigated the effect of SB on the production of cytokines in a human T-cell line, MOLT-4 cells, and mouse peritoneal macrophages. The MOLT-4 cells were cultured for 24 h in the presence or absence of SB plus concanavalin (con) A. SB plus con A significantly increased the level of interleukin (IL)-2, IL-4 and interferon (IFN)-gamma production compared with that of con A alone (approximately 1.79-fold for IL-2, 2-fold for IL-4, and 1.85-fold for IFN-gamma, p < 0.05). SB plus recombinant IFN-gamma (rIFN-gamma) increased the level of IL-12 and NO production compared with rIFN-gamma alone. In addition, SB plus rIFN-gamma increased the level the iNOS expression on mouse peritoneal macrophages. Overall, SB may have an immune-enhancement effect through cytokine production.
Kim
Su-Jin
SJ
College of Pharmacy, Wonkwang University, Iksan-city, Jeonbuk, Republic of Korea.
Park
Jeong-Su
JS
Myung
Noh-Yil
NY
Moon
Phil-Dong
PD
Choi
In-Young
IY
An
Hyo-Jin
HJ
Kim
Na-Hyung
NH
Na
Ho-Jeong
HJ
Kim
Dong-Hyun
DH
Kim
Min-cheol
MC
An
Nyeon-Hyoung
NH
Kim
Il-Kwang
IK
Lee
Joo-Young
JY
Jeong
Hyun-Ja
HJ
Um
Jae-Young
JY
Kim
Hyung-Min
HM
Hong
Seung-Heon
SH
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000032:analysis / Q000276:immunology; D010936:Plant Extracts / Q000276:immunology / Q000494:pharmacology*; D032266:Scrophularia / Q000276:immunology*
D016207:Cytokines; D010936:Plant Extracts; D003208:Concanavalin A; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II