title: Corticotropin-releasing factor decreases IL-18 in the monocyte-derived dendritic cell.
abstract: Recent evidence suggests that crosstalk between mast cells, nerves and keratinocytes might be involved in the exacerbation of inflammatory conditions by stress, but the mechanism by which this occurs remains unclear. Corticotropin-releasing factor (CRF), which activates the hypothalamo-pituitary-adrenal (HPA) axis under stress, also has pro-inflammatory peripheral effects. However, there have been no reports about CRF receptor expression and the functional role of CRF in the dendritic cell (DC), which is considered to be the link between allergen uptake and the clinical manifestations of allergic diseases, such as atopic dermatitis. The purpose of this study was to investigate the expression of CRF receptors and the functional role of CRF in the monocyte-derived DC (MoDC) of atopic dermatitis patients and non-atopic healthy controls. In this study, mRNAs for CRF-R1alpha and 1beta, as well as the CRF-R1 protein, were detected in MoDCs. CRF-R2alpha (but not R2beta or R2gamma) mRNA and the CRF-R2 protein were present in MoDCs. Exposure of DCs to CRF resulted in a decrease of IL-18 in both atopic dermatitis patients and non-atopic healthy controls. However, CRF did not alter the expression of IL-6, CCL17, CCL18, and CCL22. Therefore, our results demonstrate that CRF could modulate immune responses by acting directly upon DCs.
authors.affiliation: Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Yeon Sook
authors.initials: YS
authors.lastname: Kwon
authors.forename: Chang Ook
authors.initials: CO
authors.lastname: Park
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Oh
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Wen Hao
authors.initials: WH
authors.lastname: Wu
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Chang
authors.forename: Min-Geol
authors.initials: MG
authors.lastname: Lee
authors.forename: Kwang Hoon
authors.initials: KH
authors.lastname: Lee
mesh_terms: D016022:Case-Control Studies; D002478:Cells, Cultured; D054414:Chemokine CCL17 / Q000378:metabolism; D054422:Chemokine CCL22 / Q000378:metabolism; D019742:Chemokines, CC / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000502:physiology*; D003713:Dendritic Cells / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000378:metabolism / Q000473:pathology; D006801:Humans; D020382:Interleukin-18 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018019:Receptors, Corticotropin-Releasing Hormone / Q000378:metabolism
chemical_list: C111792:CCL18 protein, human; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D019742:Chemokines, CC; D020382:Interleukin-18; D015850:Interleukin-6; D020033:Protein Isoforms; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D003346:Corticotropin-Releasing Hormone