[Mixture effects to vitellogenin induction by four environmental estrogens in freshwater fish].
Combined effects of estrogenic compounds to high risk life-form were demonstrated, and the study method on combined effects of environmental contaminant mixture and environmental risk assessment were also discussed. A nonlinear aggression was determined on the changes of plasma vitellogenin concentration in crucian carp via the environmental pollutant exposure concentration, and the concentration-response relationship was determined for the single chemical of estradiol, ethynylestradiol, bisphenol A, and octylphenol, and that of the mixed compounds at equipotent concentrations. The combined effects of the four xenoestrogens were tested using indices of mixture toxicity and the biomathematical models of concentration addition or independent action. Weibull function was indicated as the best-fit function (R2 > or = 0.92) with curve estimation, the 95% confidence limits of effect concentration was achieved using the bootstrap method, the media effective concentration (EC50) with 95% confidence interval was 0.007 9 (0.006 8-0.010 0), 0.098 7 (0.090 0-0.111 0), 63.50 (56.58-70.62) and 250.59 (228.46-271.99) Mg x L(-1) respectively. Results suggested that estrogenic chemicals acted together in an additive manner and the combined effects can be accurately predicted in whole range of exposure concentration by the concept of similar joint action or concentration addition.It is proved as a scientific and feasible method to apply the model of joint action whereas the outcome of indices with a great deal of indetermination.
Zhang
Hui
H
Department of Municipal Engineering, Southeast University, Nanjing 210096, China. zh6862@seu.edu.cn
Kong
Fan-Xiang
FX
Wang
Shi-He
SH
Yu
Yang
Y
Zhang
Min
M
Chen
Mei-Jun
MJ
Tan
Xiao
X
Qian
Shan-Qin
SQ
D000818:Animals; D001559:Benzhydryl Compounds; D002347:Carps / Q000378:metabolism*; D004357:Drug Synergism; D004785:Environmental Pollutants / Q000633:toxicity*; D004958:Estradiol / Q000633:toxicity; D004967:Estrogens / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000633:toxicity; D005618:Fresh Water; D008297:Male; D008962:Models, Theoretical; D010636:Phenols / Q000633:toxicity; D018675:Toxicity Tests / Q000379:methods; D014819:Vitellogenins / Q000096:biosynthesis / Q000097:blood*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D004958:Estradiol; D004967:Estrogens; D004997:Ethinyl Estradiol; D010636:Phenols; D014819:Vitellogenins; C474055:octylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds