title: [Expression and enzymatic activity of glutathione s-transferase in tissues of Boophilus microplus females].
abstract: Cellular detoxification and excretion enzymes are important to the maintenance of cellular homeostasis. In this work mRNA transcription, protein expression and enzymatic activity of Glutathione S-transferases (GSTs), enzymes involved in the excretion of endo and xenobiotic compounds were analyzed. These parameters are elements believed to protect cells against chemical toxicity and oxidative stress in different tissues (salivary gland, ovary and synganglion) from partially engorged females and engorged females of Boophilus microplus. The results presented showed elevated GST activity in partially engorged females. The enzymatic activity decreased during the preoviposition period in engorged females. GST mRNA transcription was detected in salivary glands and synganglion from partially engorged and engorged females, but not in ovary. The results of this work help to elucidate the role of GST in tick development and assist in the understanding of the importance of GST in tick females during the preparation for oviposition.
authors.affiliation: Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, Campus do Vale, Porto Alegre, RS, Brazil.
authors.forename: Daniela R J
authors.initials: DR
authors.lastname: de Freitas
authors.forename: Itabajara da S
authors.initials: Ida S
authors.lastname: Vaz
authors.forename: Aoi
authors.initials: A
authors.lastname: Masuda
mesh_terms: D000818:Animals; D005260:Female; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000378:metabolism*; D013987:Ticks / Q000201:enzymology*
chemical_list: D005982:Glutathione Transferase