title: [Studies on the possible application of molecular methods in diagnosing carriers and in similarity analysis of group B streptococci (Streptococcus agalactiae)].
abstract: The most popular method of GBS identification in Poland currently is by culturing on enriched agar and verifying the Lancefield Group using special latex agglutination kits. However, the classical methods are time-consuming and their sensitivity is insufficient therefore it is becoming more common to try and apply molecular methods which are characterized by high sensitivity and rapid results. Moreover, molecular methods give us the possibility to carry out epidemiological investigations and gene detection, for instance for antibiotic resistance. It was confirmed that PCR and FISH procedures may be effective in rapid detection of GBS. Thanks to RAPD methods we showed that newborns born to colonized mothers were colonized by GBS strains which originated from the mother, irrespective of the way and the course of labour. Additionally, we detected GBS colonization in children who were born to mothers who were not colonized by GBS. These children were probably colonized with strains coming from hospital environment. More studies are needed to elucidate the route of transmission and the role of colonization of the medical staff. Using multiplex PCR we showed the presence of ermA, ermB and ermC genes in phenotypically confirmed MLS, GBS strains.
authors.affiliation: Katedra Mikrobiologii Collegium Medicum UJ Kraków. mbrzych@cm-uj.krakow.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Brzychczy-Włoch
authors.forename: Magdalena
authors.initials: M
authors.lastname: Strus
authors.forename: Dorota
authors.initials: D
authors.lastname: Pawlik
authors.forename: Tomasz
authors.initials: T
authors.lastname: Gosiewski
authors.forename: Rytlewski
authors.initials: R
authors.lastname: Krzysztof
authors.forename: Artur
authors.initials: A
authors.lastname: Drzewiecki
authors.forename: Ryszard
authors.initials: R
authors.lastname: Lauterbach
authors.forename: Piotr B
authors.initials: PB
authors.lastname: Heczko
mesh_terms: D000328:Adult; D003428:Cross Infection / Q000382:microbiology* / Q000635:transmission*; D004352:Drug Resistance, Microbial; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D017757:Infectious Disease Transmission, Professional-to-Patient / Q000379:methods; D018445:Infectious Disease Transmission, Vertical / Q000145:classification*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013290:Streptococcal Infections / Q000382:microbiology* / Q000635:transmission*; D013292:Streptococcus agalactiae / Q000145:classification* / Q000235:genetics