Involvement of platelet-derived growth factor receptor-beta in maintenance of mesenchyme and sensory epithelium of the neonatal mouse inner ear.
Platelet-derived growth factor receptor (PDGFR) signaling has been demonstrated to play a pivotal role in early embryonic development. Although the expression of PDGF in the inner ear has been studied by RT-PCR, how PDGFR is involved there remains largely unclear. In the current study, we used the antagonistic anti-PDGFR-beta antibody, APB5, to investigate the role of PDGFR-beta in the neonatal mouse inner ear. PDGFR-beta was detected immunohistochemically in the mesenchymal tissue adjacent to the sensory epithelium of the inner ear, and a ligand for PDGFR-beta was detected around the sensory epithelium. To determine whether this expression plays a functional role, we injected APB5 into neonates to block the function of PDGFR-beta. Mesenchymal tissue defects and abnormal capillaries with irregular shapes, especially in the cochlear lateral wall, were detected in APB5-treated mice. The results of a TUNEL assay revealed that not only the adjacent mesenchymal cells but also the sensory epithelial cells underwent cell death. These results indicate that PDGFR-beta signals are required for the survival of the capillary and mesenchymal cells in the neonatal mouse inner ear and also indirectly implicate these signals in the survival of the sensory epithelium.
Hayashi
Hisamitsu
H
Department of Otolaryngology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu-shi, Gifu 501-1194, Japan. hh@gifu-u.ac.jp
Kunisada
Takahiro
T
Takakura
Nobuyuki
N
Aoki
Mitsuhiro
M
Mizuta
Keisuke
K
Ito
Yatsuji
Y
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D017209:Apoptosis; D002196:Capillaries / Q000033:anatomy & histology; D002470:Cell Survival; D007758:Ear, Inner / Q000033:anatomy & histology* / Q000098:blood supply / Q000502:physiology*; D004848:Epithelium / Q000033:anatomy & histology / Q000473:pathology; D005260:Female; D007150:Immunohistochemistry; D008648:Mesoderm / Q000033:anatomy & histology / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D015398:Signal Transduction
D000911:Antibodies, Monoclonal; D020797:Receptor, Platelet-Derived Growth Factor beta