Chemoenzymatic synthesis of GDP-azidodeoxymannoses: non-radioactive probes for mannosyltransferase activity.
GDP-2-, 3-, 4- or 6-azidomannoses can be successfully prepared from the corresponding azidomannose-1-phosphates and GTP using the enzyme GDP-Mannosepyrophosphorylase (GDP-ManPP) from Salmonella enterica and may serve as useful probes for mannosyltransferase activity.
Marchesan
Silvia
S
Department of Chemistry, University College London, 20 Gordon Street, London, UK WC1H 0AJ.
Macmillan
Derek
D
D002236:Carbohydrate Conformation; D004789:Enzyme Activation; D006155:Guanosine Diphosphate Mannose / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D006160:Guanosine Triphosphate / Q000737:chemistry; D008364:Mannosyltransferases / Q000032:analysis / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D019779:Salmonella enterica / Q000201:enzymology
D015335:Molecular Probes; D011994:Recombinant Proteins; D006155:Guanosine Diphosphate Mannose; D006160:Guanosine Triphosphate; D008364:Mannosyltransferases