The scaffold protein c-Jun NH2-terminal kinase-associated leucine zipper protein regulates cell migration through interaction with the G protein G(alpha 13).
Scaffold proteins for MAP kinase (MAPK) signalling modules play an important role in the specific and efficient signal transduction of the relevant MAPK cascades. Here, we investigated the function of the scaffolding protein c-Jun NH(2)-terminal kinase (JNK)-associated leucine zipper protein (JLP) by depleting it in cultured cells using a short hairpin RNA (shRNA) against human JLP. HeLa and DLD-1 cells stably expressing the shRNA showed a defect in cell migration. The re-expression of full-length shRNA-resistant mouse JLP rescued the impaired cell migration of the JLP-depleted HeLa cells; whereas, a C-terminal deletion mutant of mouse JLP, which failed to bind the G protein G(alpha13), showed little or no effect on the cell migration defect. Furthermore, although a constitutively active G(alpha13) enhanced the migration of control HeLa cells, the G(alpha13)-induced cell migration was significantly suppressed in the JLP-depleted HeLa cells. Taken together, these results suggest that JLP regulates cell migration through an interaction with G(alpha13).
Gantulga
Davaakhuu
D
Department of Molecular and Cellular Biology, Cancer Research Institute, Kanazawa University, Kanazawa, 920-0934, Japan.
Tuvshintugs
Baljinnyam
B
Endo
Yoshio
Y
Takino
Takahisa
T
Sato
Hiroshi
H
Murakami
Seishi
S
Yoshioka
Katsuji
K
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D044365:GTP-Binding Protein alpha Subunits, G12-G13 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D015398:Signal Transduction; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; C111047:SPAG9 protein, human; C468928:Spag9 protein, mouse; D044365:GTP-Binding Protein alpha Subunits, G12-G13; D020830:rac1 GTP-Binding Protein