title: Increase in mitochondrial density within axons and supporting cells in response to demyelination in the Plp1 mouse model.
abstract: We used the Plp1-overexpressing transgenic mouse model to investigate whether progressive demyelination of axons results in adaptive changes involving mitochondria within the axons. These models have myelinated axons from birth but gradually lose myelin and develop axonal loss associated with progressive neurological disability analogous to patients with secondary progressive mulltiple sclerosis (SPMS). At 1 and 2 months, electron microscopy demonstrated a significant increase in intraaxonal mitochondrial density in the homozygous line 72 Plp1-overexpressing mice compared with wild type (1.43 +/- 0.31 vs. 0.84 +/- 0.16 microm(-3), P = 0.031; 1.66 +/- 0.11 vs. 0.92 +/- 0.43 microm(-3), P = 0.02) and a significant increase at 1 and 4 months in the density of mitochondria in the surrounding cells in the same mice (1.86 +/- 0.31 vs. 0.81 +/- 0.30 microm(-3), P = 0.006; 2.77 +/- 0.44 vs. 1.37 +/- 0.42 microm(-3), P = 0.016). At both 1 and 4 months, COX histochemistry and time-lapse histochemistry demonstrated a significant increase in mitochondrial activity and rate of mitochondrial activity in the homozygous Plp1-overexpressing mouse optic nerve compared with the wild type (112.37 +/- 11.9 vs. 136.89 +/- 9.1 MeanD, P = 0.006; 128.02 +/- 3.0 vs. 188.77 +/- 9.7 MeanD P < 0.001; Rate -0.78 +/- 0.25 vs. -0.58 +/- 0.15 MeanD min(-1), P < 0.001; -1.48 +/- 0.15 vs. 0.51 +/- 0.17 MeanD min(-1), P < 0.001, respectively). We propose that adaptive changes involving mitochondria occur within CNS axons in Plp1-overexpressing mice, which may be detrimental to long-term viability. Analogous changes occurring in chronically demyelinated axons in MS lesions would be one mechanism increasing axonal vulnerability in SPMS.
authors.affiliation: Department of Neurology, Medical School, Newcastle Upon Tyne, United Kingdom.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Hogan
authors.forename: Kathryn
authors.initials: K
authors.lastname: White
authors.forename: Julia
authors.initials: J
authors.lastname: Edgar
authors.forename: Alison
authors.initials: A
authors.lastname: McGill
authors.forename: Saadia
authors.initials: S
authors.lastname: Karim
authors.forename: Mark
authors.initials: M
authors.lastname: McLaughlin
authors.forename: Ian
authors.initials: I
authors.lastname: Griffiths
authors.forename: Doug
authors.initials: D
authors.lastname: Turnbull
authors.forename: Philip
authors.initials: P
authors.lastname: Nichols
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000473:pathology / Q000648:ultrastructure*; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure*; D003711:Demyelinating Diseases / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D051379:Mice; D008822:Mice, Transgenic; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure*; D009103:Multiple Sclerosis / Q000378:metabolism / Q000473:pathology; D018991:Myelin Proteolipid Protein / Q000235:genetics*; D009900:Optic Nerve / Q000378:metabolism / Q000648:ultrastructure; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D018991:Myelin Proteolipid Protein; C047516:Plp1 protein, mouse; D011451:Prostaglandin-Endoperoxide Synthases