title: [Establishment of a nude mouse model of nasopharyngeal carcinoma lymph node metastasis and screening of the metastasis-related signature genes].
abstract: OBJECTIVE
  To establish a nude mouse model of nasopharyngeal carcinoma (NPC) lymph node metastasis and screen the signature genes associated with the metastasis.
  
  
  METHODS
  The NPC 5-8F-EGFP cells were inoculated into nude mice, from which a 5-8F-LN cell line with lymph node metastasis potential was obtained. The lymphatic metastasis-related signature genes of breast cancer and head and neck squamous cell carcinoma were screened by data mining method.
  
  
  RESULTS
  The NPC cell lines 5-8F and 6-10B showed 307 differentially expressed genes by microarray analysis, from which 20 overlapping genes were identified, and 3 overexpressed genes were found with probable metastasis potential, namely the ADM, IRF1, and CAV1 genes. Quantitative RT-PCR validated the data mining results in the 5-8F-EGFP, 6-10B-EGFP, NP69, and 5-8F-LN cell lines. The 3 NPC cell lines 5-8F-EGFP, 6-10B-EGFP and 5-8F-LN showed significantly higher expressions of IRF1 than NP69 cells (P=0.008, 0.022, and 0.006, respectively. The expression level of CAV1 in 5-8F-EGFP cells was significantly higher than that in 6-10B-EGFP cells (P=0.014), but ADM expression showed no significant difference between the 4 cell lines.
  
  
  CONCLUSIONS
  IRF1 may play an important role in the progression of NPC. The overexpression of CAV1 in 5-8F-EGFP cells can be associated with the high metastatic potential of the cells.
authors.affiliation: Cancer Institute, Southern Medical University, Guangzhou, China. lenglei_1981@126.com
authors.forename: Lei
authors.initials: L
authors.lastname: Leng
authors.forename: Teng-fei
authors.initials: TF
authors.lastname: Liu
authors.forename: Zhong-xi
authors.initials: ZX
authors.lastname: Huang
authors.forename: Wei-bing
authors.initials: WB
authors.lastname: Xie
authors.forename: Kai-tai
authors.initials: KT
authors.lastname: Yao
mesh_terms: D053607:Adrenomedullin / Q000235:genetics; D000818:Animals; D051242:Caveolin 1 / Q000235:genetics; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006801:Humans; D050856:Interferon Regulatory Factor-1 / Q000235:genetics; D008207:Lymphatic Metastasis; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D009368:Neoplasm Transplantation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous
chemical_list: C495541:Cav1 protein, mouse; D051242:Caveolin 1; D050856:Interferon Regulatory Factor-1; C494258:Irf1 protein, mouse; D053607:Adrenomedullin