title: Co-up-regulation of three P450 genes in response to permethrin exposure in permethrin resistant house flies, Musca domestica.
abstract: BACKGROUND
  Insects may use various biochemical pathways to enable them to tolerate the lethal action of insecticides. For example, increased cytochrome P450 detoxification is known to play an important role in many insect species. Both constitutively increased expression (overexpression) and induction of P450s are thought to be responsible for increased levels of detoxification of insecticides. However, unlike constitutively overexpressed P450 genes, whose expression association with insecticide resistance has been extensively studied, the induction of P450s is less well characterized in insecticide resistance. The current study focuses on the characterization of individual P450 genes that are induced in response to permethrin treatment in permethrin resistant house flies.
  
  
  RESULTS
  The expression of 3 P450 genes, CYP4D4v2, CYP4G2, and CYP6A38, was co-up-regulated by permethrin treatment in permethrin resistant ALHF house flies in a time and dose-dependent manner. Comparison of the deduced protein sequences of these three P450s from resistant ALHF and susceptible aabys and CS house flies revealed identical protein sequences. Genetic linkage analysis located CYP4D4v2 and CYP6A38 on autosome 5, corresponding to the linkage of P450-mediated resistance in ALHF, whereas CYP4G2 was located on autosome 3, where the major insecticide resistance factor(s) for ALHF had been mapped but no P450 genes reported prior to this study.
  
  
  CONCLUSIONS
  Our study provides the first direct evidence that multiple P450 genes are co-up-regulated in permethrin resistant house flies through the induction mechanism, which increases overall expression levels of P450 genes in resistant house flies. Taken together with the significant induction of CYP4D4v2, CYP4G2, and CYP6A38 expression by permethrin only in permethrin resistant house flies and the correlation of the linkage of the genes with resistance and/or P450-mediated resistance in resistant ALHF house flies, this study sheds new light on the functional importance of P450 genes in response to insecticide treatment, detoxification of insecticides, the adaptation of insects to their environment, and the evolution of insecticide resistance.
authors.affiliation: Department of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, USA. fangzhudy@uky.edu
authors.forename: Fang
authors.initials: F
authors.lastname: Zhu
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Lee
authors.initials: L
authors.lastname: Zhang
authors.forename: Nannan
authors.initials: N
authors.lastname: Liu
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D006793:Houseflies / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D008658:Inactivation, Metabolic / Q000235:genetics; D007305:Insecticide Resistance / Q000187:drug effects / Q000235:genetics; D007306:Insecticides / Q000493:pharmacokinetics / Q000633:toxicity*; D008297:Male; D008969:Molecular Sequence Data; D026023:Permethrin / Q000493:pharmacokinetics / Q000633:toxicity*; D015854:Up-Regulation / Q000187:drug effects* / Q000235:genetics*
chemical_list: D007306:Insecticides; D026023:Permethrin; D003577:Cytochrome P-450 Enzyme System