title: Viability and function in lymphocytes cultured from the horse, chicken, and mouse: effects of different leukocyte enrichment techniques.
abstract: Methods of lymphocyte enrichment tend to vary across species, with the most common techniques employed being density-gradient separation and erythrocyte lysis buffer enrichment. In this study, we assessed lymphocyte viability and proliferation of avian, equine, and murine lymphocytes enriched by a commercial density-gradient technique and under identical, standardized culture conditions. The results of this study clearly show that, under identical enrichment and culture conditions, lymphocyte viability and function can be quite different among the equine, bird, and mouse species. Secondly, the type of enrichment technique employed in the mouse can impact the quality of the immune data generated.
authors.affiliation: Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute, Blacksburg, VA 24061-0342, USA.
authors.forename: A
authors.initials: A
authors.lastname: Mustafa
authors.forename: L
authors.initials: L
authors.lastname: Gillmeister
authors.forename: W P
authors.initials: WP
authors.lastname: Hernandez
authors.forename: C T
authors.initials: CT
authors.lastname: Larsen
authors.forename: S
authors.initials: S
authors.lastname: Witonsky
authors.forename: S D
authors.initials: SD
authors.lastname: Holladay
authors.forename: R P
authors.initials: RP
authors.lastname: Kerr
authors.forename: S A
authors.initials: SA
authors.lastname: Ahmed
authors.forename: A
authors.initials: A
authors.lastname: Santo
authors.forename: R M
authors.initials: RM
authors.lastname: Gogal
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D002645:Chickens; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006736:Horses; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000166:cytology* / Q000502:physiology*; D008297:Male; D051379:Mice
chemical_list: D003208:Concanavalin A