title: Distribution of secretory inhibitor of platelet microbicidal protein among anaerobic bacteria isolated from stool of children with diarrhea.
abstract: OBJECTIVE
  To study the secretory inhibitor of platelet microbicidal protein (SIPMP) phenotypes of faecal anaerobic isolates from patients with diarrhea.
  
  
  METHODS
  Faecal isolates of anaerobic bacteria (B. fragilis, n = 42; B. longum, n = 70; A. israelii, n = 21; E. lentum, n = 12) from children with diarrhea were tested. SIPMP production was tested by inhibition of platelet microbicidal protein (PMP) bioactivity against B. subtilis and was expressed as percentage of inhibition of PMP bactericidal activity.
  
  
  RESULTS
  Among anaerobic isolates 80% of B. longum strains, 85.7% of A. israelii strains, 50% of E. lentum strains and 92.86% of B. fragilis strains were SIPMP-positive. The isolated anaerobic organisms demonstrated SIPMP production at a mean level of 13.8% +/- 0.7%, 14.7% +/- 1.8%, 3.9% +/- 0.9% (P < 0.05) and 26.8% +/- 7.5% (P < 0.05) for bifidobacteria, A. israelii, E. lentum and B. fragilis, respectively.
  
  
  CONCLUSIONS
  Data from the present study may have significant implications in understanding the pathogenesis of microecological disorders in the intestine, as well as for future improvement in the prevention and therapy of anaerobe-associated infections.
authors.affiliation: Department of Human Microbiology, Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Pionerskaya st., 11, Orenburg, Russia. mil-for@rambler.ru
authors.forename: Iuri B
authors.initials: IB
authors.lastname: Ivanov
authors.forename: Viktor A
authors.initials: VA
authors.lastname: Gritsenko
mesh_terms: D000190:Actinomyces / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D001421:Bacteria, Anaerobic / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology*; D001424:Bacterial Infections / Q000382:microbiology; D001426:Bacterial Proteins / Q000502:physiology; D001441:Bacteroides fragilis / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D001644:Bifidobacterium / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D005051:Eubacterium / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D014774:Virulence / Q000502:physiology; D001620:beta-Thromboglobulin / Q000037:antagonists & inhibitors*
chemical_list: D001426:Bacterial Proteins; C061615:PPBP protein, human; D001620:beta-Thromboglobulin