title: First record of Perkinsus olseni, a protozoan parasite infecting the commercial clam Ruditapes decussatus in Spanish Mediterranean waters.
abstract: We present the first record in Spanish Mediterranean waters of the protozoan parasite Perkinsus olseni infecting the clam Ruditapes decussatus. Perkinsus infection was detected all year around albeit at a low level of infection intensity. Histological analysis, induction of zoospores and in situ hybridisation assay confirmed the presence of Perkinsus sp. The identity of the parasite was determined by species-specific PCR assay in DNA samples obtained from infected clams. Sequencing of amplified fragments showed 100% identity to the ITS region of P. olseni. We confirmed for the first time the presence of P. olseni in Spanish Mediterranean waters.
authors.affiliation: IRTA-Sant Carles de la Ràpita, Sant Carles de la Ràpita, Spain. laurence.elandaloussi@irta.es
authors.forename: Laurence M
authors.initials: LM
authors.lastname: Elandaloussi
authors.forename: Noèlia
authors.initials: N
authors.lastname: Carrasco
authors.forename: Ana
authors.initials: A
authors.lastname: Roque
authors.forename: Karl
authors.initials: K
authors.lastname: Andree
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Furones
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000166:cytology / Q000469:parasitology*; D008522:Mediterranean Sea; D010271:Parasites / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000737:chemistry; D017423:Sequence Analysis, RNA; D013030:Spain
chemical_list: D012335:RNA, Ribosomal