title: Chiral separation of the plant lignan matairesinol by capillary electrophoresis.
abstract: Lignans are dimeric phenylpropanoid compounds in plants that enjoy increasing medicinal interest because of their phytoestrogen activity. Lignans are chiral compounds and for most natural occurring lignans, chirality is not known. Separation of racemic matairesinol by CE in a non-coated silica capillary with carboxymethyl-beta-cyclodextrin as chiral selector in phosphate buffer was successful. Electrolyte and selector concentrations and pH were systematically optimized in order to obtain baseline separation and short analysis times. Matairesinol from safflower fruit was determined as (-)-enantiomer. Quantitation results for matairesinol with the optimized method after calibration with authentic lignan were very similar to those by HPLC. The limit of detection is 2 microg/mL sample by DAD detection.
authors.affiliation: Institute of Pharmacy, Biosciences, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Müller
authors.forename: Yahya
authors.initials: Y
authors.lastname: Mrestani
authors.forename: Reinhard
authors.initials: R
authors.lastname: Neubert
authors.forename: Birgit
authors.initials: B
authors.lastname: Dräger
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D005663:Furans; D017705:Lignans; C068935:matairesinol