title: In situ detection of live cancer cells by using bioprobes based on Au nanoparticles.
abstract: We fabricate the high-performance probes based on Au nanoparticles (AuNP) for detection of live cancer cell. AuNP were synthesized with narrow sized distribution (ca. 10 nm) by Au salt reduction method and deposited onto the aminated substrate as a cross-linker and hot spot. Herein, AuNP has enabled the easy and efficient immobilization of the antibody (Cetuximab), which can selectively interact with epidermal growth factor receptor (EGFR) on the surface of epidermal cancer, as detecting moiety onto the AuNP-deposited substrate without nanolithography process. After conjugation of Cetuximab with AuNP-deposited substrate, Cetuximab-conjugated probe as a live cancer cell detector (LCCD) could detect EGFR-highexpressed A431 cells related to epithelial cancer with 54-times larger specificity and sensitivity in comparison with EGFR-deficient MCF7 cells. This implies that AuNP-based probes demonstrate abundant potentials for detection and separation of small biomolecules, cells and other chemicals.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Republic of Korea.
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
authors.forename: Kilho
authors.initials: K
authors.lastname: Eom
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Lim
authors.forename: Jinsung
authors.initials: J
authors.lastname: Park
authors.forename: Yoonah
authors.initials: Y
authors.lastname: Kang
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Yoon
authors.forename: Sungsoo
authors.initials: S
authors.lastname: Na
authors.forename: Eui Kwan
authors.initials: EK
authors.lastname: Koh
authors.forename: Jin-Suck
authors.initials: JS
authors.lastname: Suh
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
authors.forename: Tae Yun
authors.initials: TY
authors.lastname: Kwon
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
mesh_terms: D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D015335:Molecular Probes; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D015335:Molecular Probes; D006046:Gold