Affinity precipitation of proteins using metal chelates.
Metal affinity precipitation has been successfully developed as a simple purification process for the proteins that have affinity for the metal ions. The copolymers of vinylimidazole with N-isopropylacrylamide are easily synthesized by radical polymerization. When loaded with Cu(II) and Ni(II) ions, these copolymers are capable of selectively precipitating proteins with natural metal-binding groups or histidine-tagged recombinant proteins.
Kumar
Ashok
A
Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, India.
Galaev
Igor Y
IY
Mattiasson
Bo
B
D002614:Chelating Agents / Q000737:chemistry*; D011232:Chemical Precipitation; D002846:Chromatography, Affinity / Q000379:methods*; D008670:Metals / Q000737:chemistry*
D002614:Chelating Agents; D008670:Metals