title: Single nucleotide polymorphism (SNP) in prion protein gene (PRNP) exon-3 in Gaddi sheep.
abstract: Nine PCR-SSCP conformations in PRNPthe exon-3 genomic DNA segment covering codons 136, 154 and 171 were observed. DNA sequences revealed 9 unique variants (DQ885789-DQ885796). At codon 171 of exon-3, two alleles, Q and R, had a frequency of 0.336 and 0.664, respectively. Genotypes QQ, QR and RR were observed in 25.86, 15.51 and 58.62% of animals, respectively, showing deviation from Hardy-Weinberg equilibrium. At codon 154, all genotypes were RR. At codon 136 AA, AV and W genotypes were 86.21, 10.34 and 3.44%, respectively, and frequencies of the alanine (A) and valine (V) alleles were 0.914 and 0.086, respectively, where the W genotype had lower frequency than expected. Polymorphisms were also observed at codons 137, 143 and 173 of exon-3.
authors.forename: N
authors.initials: N
authors.lastname: Gupta
authors.forename: S
authors.initials: S
authors.lastname: Choudhary
authors.forename: G
authors.initials: G
authors.lastname: Malik
authors.forename: A
authors.initials: A
authors.lastname: Pandey
authors.forename: S C
authors.initials: SC
authors.lastname: Gupta
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004247:DNA / Q000235:genetics; D005091:Exons; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D011328:Prions / Q000235:genetics*; D012756:Sheep
chemical_list: D011328:Prions; D004247:DNA