title: Triglyceride-rich lipoprotein lipolysis releases neutral and oxidized FFAs that induce endothelial cell inflammation.
abstract: Triglyceride-rich lipoprotein (TGRL) lipolysis products provide a pro-inflammatory stimulus that can alter endothelial barrier function. To probe the mechanism of this lipolysis-induced event, we evaluated the pro-inflammatory potential of lipid classes derived from human postprandial TGRL by lipoprotein lipase (LpL). Incubation of TGRL with LpL for 30 min increased the saturated and unsaturated FFA content of the incubation solutions significantly. Furthermore, concentrations of the hydroxylated linoleates 9-hydroxy ocatadecadienoic acid (9-HODE) and 13-HODE were elevated by LpL lipolysis, more than other measured oxylipids. The FFA fractions elicited pro-inflammatory responses inducing TNFalpha and intracellular adhesion molecule expression and reactive oxygen species (ROS) production in human aortic endothelial cells (HAECs). The FFA-mediated increase in ROS was blocked by both the cytochrome P450 2C9 inhibitor sulfaphenazole and NADPH oxidase inhibitors. Compared with linoleate, 13-HODE was found to be a more potent inducer of ROS production in HAECs, an activity that was insensitive to both NADPH oxidase and cytochrome P450 inhibitors. Therefore, although the oxidative metabolism of FFA in endothelial cells can produce inflammatory responses, TGRL lipolysis can also release preformed mediators of oxidative stress (e.g., HODEs) that may influence endothelial cell function in vivo by stimulating intracellular ROS production.
authors.affiliation: Division of Endocrinology, Clinical Nutrition, and Vascular Medicine, University of California, Davis, Davis, CA, USA. lwang@ucdavis.edu
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.forename: Rajan
authors.initials: R
authors.lastname: Gill
authors.forename: Theresa L
authors.initials: TL
authors.lastname: Pedersen
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Higgins
authors.forename: John W
authors.initials: JW
authors.lastname: Newman
authors.forename: John C
authors.initials: JC
authors.lastname: Rutledge
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D008066:Lipolysis / Q000502:physiology*; D008071:Lipoprotein Lipase / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism*; D010084:Oxidation-Reduction; D019518:Postprandial Period / Q000502:physiology; D017382:Reactive Oxygen Species; D014280:Triglycerides / Q000097:blood / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D005230:Fatty Acids, Nonesterified; D018836:Inflammation Mediators; D008074:Lipoproteins; D017382:Reactive Oxygen Species; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D008071:Lipoprotein Lipase