title: Magnetic resonance imaging assessment of regional cerebral blood flow after asphyxial cardiac arrest in immature rats.
abstract: Cerebral blood flow (CBF) alterations after asphyxial cardiac arrest (CA) are not defined in developmental animal models or humans. We characterized regional and temporal changes in CBF from 5 to 150 mins after asphyxial CA of increasing duration (8.5, 9, 12 min) in postnatal day (PND) 17 rats using the noninvasive method of arterial spin-labeled magnetic resonance imaging (ASL-MRI). We also assessed blood-brain barrier (BBB) permeability, and evaluated the relationship between CBF and mean arterial pressure after resuscitation. After all durations of asphyxia CBF alterations were region dependent. After 8.5- and 9-min asphyxia, intense subcortical hyperemia at 5 min was followed by return of CBF to baseline values by 10 mins. After 12-min asphyxia, hyperemia was absent and hypoperfusion reached a nadir of 38% to 65% of baselines with the lowest values in the cortex. BBB was impermeable to gadoteridol 150 mins after CA. CBF in the 12-min CA group was blood pressure passive at 60 min assessed via infusion of epinephrine. ASL-MRI assessment of CBF after asphyxial CA in PND 17 rats reveals marked duration and region-specific reperfusion patterns and identifies possible new therapeutic targets.
authors.affiliation: Division of Pediatric Emergency Medicine, Department of Pediatrics, Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213-2583, USA. mioara.manole@chp.edu
authors.forename: Mioara D
authors.initials: MD
authors.lastname: Manole
authors.forename: Lesley M
authors.initials: LM
authors.lastname: Foley
authors.forename: T Kevin
authors.initials: TK
authors.lastname: Hitchens
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Kochanek
authors.forename: Robert W
authors.initials: RW
authors.lastname: Hickey
authors.forename: Hülya
authors.initials: H
authors.lastname: Bayir
authors.forename: Henry
authors.initials: H
authors.lastname: Alexander
authors.forename: Chien
authors.initials: C
authors.lastname: Ho
authors.forename: Robert S B
authors.initials: RS
authors.lastname: Clark
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001237:Asphyxia / Q000473:pathology* / Q000503:physiopathology*; D001794:Blood Pressure; D001812:Blood-Brain Barrier; D001835:Body Weight; D002560:Cerebrovascular Circulation; D006323:Heart Arrest / Q000473:pathology* / Q000503:physiopathology*; D008279:Magnetic Resonance Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley