[Changes in the lipid composition of embryonic cells of the mussel Mytilus trossulus during cryopreservation].
A comparative analysis of the lipid composition of embryonic cells of the mussel Mytilus trossulus prior to, and after, cryopreservation in liquid nitrogen was carried out in the presence of two types of cryoprotectors: 1) dimethylsulfoxide and trehalose; and 2) dimethylsulfoxide, trehalose, total lipid extract from the mussel Crenomytilus grayanus, alpha-tocopherol and ascorbic acid. It was found that not only the cell viability but also the fatty acid composition of cell lipids after cryopreservation depend on the composition of cryoprotectors used. The content of saturated fatty acids, monoenic and polyenic fatty acids, the omega 3/omega 6 ratio, and the index of nonsaturation in the fatty acid composition of lipids was shown to change remarkably after cryopreservation. Possible reasons of these changes are discussed.
Korostetskiĭ
E Ia
EIa
Boroda
A V
AV
Odintsova
N A
NA
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000737:chemistry* / Q000166:cytology; D008055:Lipids / Q000032:analysis*; D049878:Mytilus / Q000737:chemistry* / Q000166:cytology; D014199:Trehalose / Q000494:pharmacology
D003451:Cryoprotective Agents; D008055:Lipids; D014199:Trehalose; D004121:Dimethyl Sulfoxide