Morin protects acute liver damage by carbon tetrachloride (CCl(4)) in rat.
The purpose of this study was to investigate possible beneficial effects of morin on CCl(4)-induced acute hepatotoxicity in rats. Rats received a single dose of CCl(4) (150 microL/100 g 1:1 in corn oil). Morin treatment (20 mg/kg) was given at 48, 24, and 2 h before CCl(4) administration. CCl(4) challenge elevated serum alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) levels, but these effects were prevented by the pretreatment of rats with morin. To identify the mechanism of protective activity of morin in CCl(4)-induced hepatotoxicity in rats, we investigated expressions of tumor necrosis factor alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and inducible nitric oxide (iNOS). The expressions of TNF-alpha, IL-1beta, IL-6, and iNOS were increased by CCl(4) treatment and increased expressions of those were decreased by morin. These findings suggest that morin prevents acute liver damage by inhibiting the production of TNF-alpha, IL-1beta, IL-6, and iNOS.
Lee
Hee Seung
HS
Clinical Research Center, College of Medicine, Inha University, Incheon, 400-712, Korea.
Jung
Kyung-Hee
KH
Hong
Sang-Won
SW
Park
In-Suh
IS
Lee
Chongmu
C
Han
Hyo-Kyung
HK
Lee
Don-Haeng
DH
Hong
Soon-Sun
SS
D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002252:Carbon Tetrachloride Poisoning / Q000473:pathology / Q000517:prevention & control*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D007249:Inflammation / Q000473:pathology; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors; D015850:Interleukin-6 / Q000096:biosynthesis; D008099:Liver / Q000473:pathology; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors; D047309:Flavones
D000975:Antioxidants; D004791:Enzyme Inhibitors; D005419:Flavonoids; D053583:Interleukin-1beta; D015850:Interleukin-6; D052247:Nitric Oxide Synthase Type II; D012313:RNA; D014409:Tumor Necrosis Factor-alpha; C008548:morin; D047309:Flavones