Mycosporine-like amino acids (MAAs) profile of a rice-field cyanobacterium Anabaena doliolum as influenced by PAR and UVR.
The mycosporine-like amino acid (MAA) profile of a rice-field cyanobacterium, Anabaena doliolum, was studied under PAR and PAR + UVR conditions. The high-performance liquid chromatographic analysis of water-soluble compounds reveals the biosynthesis of three MAAs, mycosporine-glycine (lambda (max) = 310 nm), porphyra-334 (lambda (max) = 334 nm) and shinorine (lambda (max) = 334 nm), with retention times of 4.1, 3.5 and 2.3 min, respectively. This is the first report for the occurrence of mycosporine-glycine and porphyra-334 in addition to shinorine in Anabaena strains studied so far. The results indicate that mycosporine-glycine (monosubstituted) acts as a precursor for the biosynthesis of the bisubstituted MAAs shinorine and porphyra-334. Mycosporine-glycine was under constitutive control while porphyra-334 and shinorine were induced by UV-B radiation, indicating the involvement of UV-regulated enzymes in the biotransformation of MAAs. It seems that A. doliolum is able to protect its cell machinery from UVR by synthesizing a complex set of MAAs and thus is able to survive successfully during the summer in its natural brightly lit habitats.
Singh
Shailendra P
SP
Department of Biology, Chair of Plant Ecophysiology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstrasse 5, 91058 Erlangen, Germany.
Sinha
Rajeshwar P
RP
Klisch
Manfred
M
Häder
Donat-P
DP
D017033:Anabaena / Q000528:radiation effects*; D002851:Chromatography, High Pressure Liquid; D003511:Cyclohexanols / Q000032:analysis*; D003512:Cyclohexanones / Q000032:analysis*; D003514:Cyclohexylamines / Q000032:analysis*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D012275:Oryza / Q000382:microbiology*; D010936:Plant Extracts / Q000032:analysis; D013057:Spectrum Analysis; D014466:Ultraviolet Rays
D003511:Cyclohexanols; D003512:Cyclohexanones; D003514:Cyclohexylamines; D010936:Plant Extracts; C463482:mycosporine-2-glycine; C421025:porphyra-334; C494891:shinorine; D005998:Glycine