title: Determination of protein and RNA expression levels of common housekeeping genes in a mouse model of neurodegeneration.
abstract: The choice of housekeeping proteins or genes for internal standards should be made carefully, taking into account the cell and tissue type, the experimental conditions, and the healthy/disease state(s) under consideration. Furthermore, as the correlation between transcriptional and translational levels of commonly used housekeeping genes is often discussed, this study shed light on the transcriptional levels of beta-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the translational levels of beta-actin, GAPDH, and beta-tubulin in an amyotrophic lateral sclerosis mouse model.
authors.affiliation: LAGENBIO, University of Zaragoza, Zaragoza, Spain.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Calvo
authors.forename: María
authors.initials: M
authors.lastname: Moreno-Igoa
authors.forename: Raquel
authors.initials: R
authors.lastname: Manzano
authors.forename: Laura
authors.initials: L
authors.lastname: Ordovás
authors.forename: Gema
authors.initials: G
authors.lastname: Yagüe
authors.forename: Sara
authors.initials: S
authors.lastname: Oliván
authors.forename: María J
authors.initials: MJ
authors.lastname: Muñoz
authors.forename: Pilar
authors.initials: P
authors.lastname: Zaragoza
authors.forename: Rosario
authors.initials: R
authors.lastname: Osta
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000690:Amyotrophic Lateral Sclerosis / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D004195:Disease Models, Animal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis; D051379:Mice; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D014158:Transcription, Genetic; D014404:Tubulin / Q000378:metabolism
chemical_list: D000199:Actins; D011506:Proteins; D014404:Tubulin; D012313:RNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases