title: Transpeptidation and reverse proteolysis and their consequences for immunity.
abstract: Reverse proteolysis and transpeptidation lead to the generation of polypeptide sequences that cannot be inferred directly from genome sequences as they are post-translational phenomena. These phenomena have so far received little attention although the physiological consequences may reach far. The protease-mediated synthesis of several immunodominant MHC class I antigens was recently reported, underscoring its importance to immunity. Reverse proteolytic and transpeptidation mechanisms as well as conditions that favor successful protease-catalyzed synthetic events are discussed here.
authors.affiliation: Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
authors.forename: Celia R
authors.initials: CR
authors.lastname: Berkers
authors.forename: Annemieke
authors.initials: A
authors.lastname: de Jong
authors.forename: Huib
authors.initials: H
authors.lastname: Ovaa
authors.forename: Boris
authors.initials: B
authors.lastname: Rodenko
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D002384:Catalysis; D010450:Endopeptidases / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007109:Immunity / Q000502:physiology; D008954:Models, Biological; D010455:Peptides / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D011499:Protein Processing, Post-Translational
chemical_list: D015395:Histocompatibility Antigens Class I; D010455:Peptides; D010450:Endopeptidases