title: Genetically engineered rice containing larger amounts of nicotianamine to enhance the antihypertensive effect.
abstract: Nicotianamine (NA), a metal chelator ubiquitous in higher plants, serves as an antihypertensive substance in humans. To engineer a novel antihypertensive rice that contains larger amounts of NA, the barley NA synthase gene, HvNAS1, was introduced into rice via Agrobacterium-mediated transformation. The introduced HvNAS1 was driven by pGluB-1, which induces strong gene expression in the endosperm of rice seeds. The NA content in transgenic rice seeds was up to fourfold greater than that in non-transgenic rice seeds. The Cre/loxP DNA excision (CLX) system was used to remove the selectable marker gene for antibiotic resistance. Furthermore, the transgenic rice was crossed with a cleistogamous mutant to prevent gene transfer via pollen dispersal. These two modifications may minimize public concern with regard to the use of this transgenic rice.
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Usuda
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Wada
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ishimaru
authors.forename: Takanori
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Michiko
authors.initials: M
authors.lastname: Takahashi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Nagato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Mori
authors.forename: Naoko K
authors.initials: NK
authors.lastname: Nishizawa
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D000959:Antihypertensive Agents / Q000378:metabolism*; D001383:Azetidinecarboxylic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005818:Genetic Engineering; D005819:Genetic Markers; D001467:Hordeum / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D000959:Antihypertensive Agents; D018744:DNA, Plant; D005819:Genetic Markers; D010940:Plant Proteins; C082893:nicotianamine; D001383:Azetidinecarboxylic Acid; D019883:Alkyl and Aryl Transferases; C118230:nicotianamine synthase