title: Dynamics of human respiratory virus-specific CD8+ T cell responses in blood and airways during episodes of common cold.
abstract: We determined the dynamics of CD8(+) T cells specific for influenza virus and respiratory syncytial virus in blood and tracheostoma aspirates of children during the course of respiratory infections. We showed that during localized respiratory infections the ratio of activated effector CD8(+) T cells to resting memory/naive CD8(+) T cells in peripheral blood increased significantly. Furthermore, the number of effector/memory T cells specific for respiratory viruses declined in blood and increased in the airways, suggesting that these T cells redistributed from blood to airways. T cells specific for the infecting virus were present in the airways for longer periods at increased levels than nonspecifically recruited bystander T cells. After clearance of the infection, the ratio of resting memory and naive CD8(+) T cells normalized in peripheral blood and also memory T cell numbers specific for unrelated viruses that declined during the infection due to bystander recruitment were restored. Taken together, these results showed a significant systemic T cell response during relatively mild secondary infections and extensive dynamics of virus-specific and nonspecific Ag-experienced T cells.
authors.affiliation: Division of Paediatrics, Wilhelmina Children's Hospital, Utrecht, The Netherlands.
authors.forename: Jojanneke
authors.initials: J
authors.lastname: Heidema
authors.forename: John W A
authors.initials: JW
authors.lastname: Rossen
authors.forename: Michaël V
authors.initials: MV
authors.lastname: Lukens
authors.forename: Marianne S
authors.initials: MS
authors.lastname: Ketel
authors.forename: Eva
authors.initials: E
authors.lastname: Scheltens
authors.forename: Mariette E G
authors.initials: ME
authors.lastname: Kranendonk
authors.forename: Wendy W C
authors.initials: WW
authors.lastname: van Maren
authors.forename: Anton M
authors.initials: AM
authors.lastname: van Loon
authors.forename: Henny G
authors.initials: HG
authors.lastname: Otten
authors.forename: Jan L L
authors.initials: JL
authors.lastname: Kimpen
authors.forename: Grada M
authors.initials: GM
authors.lastname: van Bleek
mesh_terms: D000293:Adolescent; D000956:Antigens, Viral / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003139:Common Cold / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D007156:Immunologic Memory; D007223:Infant; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology*; D007251:Influenza, Human / Q000097:blood / Q000276:immunology*; D008297:Male; D018357:Respiratory Syncytial Virus Infections / Q000097:blood / Q000276:immunology*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology*; D012137:Respiratory System / Q000276:immunology / Q000378:metabolism; D012229:Rhinovirus / Q000276:immunology*
chemical_list: D000956:Antigens, Viral