Evaluation of brushing cytology in the diagnosis of Helicobacter pylori gastritis.
OBJECTIVE
To evaluate clinical utility of rapid urease test (RUT), brash cytology and histology for detecting Helicobacter pylori.


METHODS
Brush cytology materials were obtained from the antrum of the stomach in 109 patients who suffered from dyspepsia and were candidates for endoscopy. RUT and histology with hematoxylin-eosin staining were performed. Infection status was established by observation of typical HP in cytology or biopsy.


RESULTS
A total of 78% ofpatients were diagnosed as positivefor HP organisms using brush cytology; 66% had histologic results positive for HP and 59% for RUT. Observation of typical organism by cytology or histology was the gold standard; 3 tests results were compared. Sensitivity of brush cytology (95%) was higher than that of histology (80.5%) and RUT (72%).


CONCLUSIONS
Gastric brushing cytology provides a sensitive, inexpensive, accurate and easy technique for rapid detection of HP infection. When additional information on severity of mucosal damage or presence of cell atypias is not necessary, histologic examination can be omitted; a cost-effective strategy for assessing HP status might consist of taking antral biopsies, the former for RUT, and performing brush cytology slides, which should be stained and examined only when the RUT result is negative.
Mostaghni
Amir Ahmad
AA
Department of Internal Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. mostaghnia@sums.ac.ir
Afarid
Mohammad
M
Eghbali
Sajjad
S
Kumar
Perikala
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D003581:Cytodiagnosis / Q000379:methods; D005753:Gastric Mucosa / Q000201:enzymology* / Q000473:pathology; D005756:Gastritis / Q000175:diagnosis* / Q000201:enzymology / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000201:enzymology; D006801:Humans; D008875:Middle Aged; D011706:Pyloric Antrum / Q000201:enzymology* / Q000473:pathology; D014510:Urease / Q000378:metabolism*
D014510:Urease