title: Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum.
abstract: There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.
authors.affiliation: Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, OR 97239, USA.
authors.forename: Andrea E
authors.initials: AE
authors.lastname: DeBarber
authors.forename: Travis
authors.initials: T
authors.lastname: Geraci
authors.forename: Vincent P
authors.initials: VP
authors.lastname: Colasurdo
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Hackenmueller
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Scanlan
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013970:Thyronines / Q000097:blood*
chemical_list: C487948:3-iodothyronamine; D013970:Thyronines