[Cloning and characterization of the chloramphenicol resistant gene of Serratia marcescens strain KMR-3].
Chloramphenicol-resistant gene was cloned and analyzed by constructing genomic DNA library of Serratia marcescens KMR-3. It showed that cloned chloramphenicol-resistant gene encoded a protein product of 397 amino acids. The protein belonged to PRK10473 protein, and it showed 92% similarity to drug resistance transporter, Bcr/CflA subfamily of Serratia proteamaculans 568. Regulation elements including promoter, terminator, Shine-Dalgarno (SD) sequence and transcription start site also were identified.
Ji
Xiuling
X
Biotechnology Research Center of Kunming University of Science and Technology, Kunming 650224, China.
Lin
Lianbing
L
Jing
Shenrong
S
Wei
Yunlin
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D002702:Chloramphenicol Resistance / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D012706:Serratia marcescens / Q000145:classification / Q000235:genetics*