title: Solid surface chemical and physical effects on the adsorption of recombinant Factor VIII.
abstract: The adsorption of a recombinant Factor VIII to silica surfaces coated to present different hydrophilic/hydrophobic, electrostatic and steric characteristics was monitored in situ, with ellipsometry. rFVIII adsorption affinity was high for hydrophobic surfaces and hydrophilic, charged surfaces. A lower affinity was recorded between rFVIII and a neutral hydrophilic surface, with substantial reduction in rFVIII adsorption to electronically neutral surfaces presenting pendant poly[ethylene oxide] chains. We conclude that steric repulsion is a requirement for minimizing rFVIII adsorption at solid surfaces.
authors.affiliation: Department of Chemical Engineering, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Yuan-Ching
authors.initials: YC
authors.lastname: Tai
authors.forename: Joseph
authors.initials: J
authors.lastname: McGuire
authors.forename: Omkar
authors.initials: O
authors.lastname: Joshi
authors.forename: D Q
authors.initials: DQ
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D055598:Chemical Phenomena; D005169:Factor VIII / Q000737:chemistry* / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D011092:Polyethylene Glycols / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D055672:Static Electricity; D013237:Stereoisomerism; D013499:Surface Properties
chemical_list: D011092:Polyethylene Glycols; D012822:Silicon Dioxide; C078147:F8 protein, human; D005169:Factor VIII