A physical map of the 1-gigabase bread wheat chromosome 3B.
As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.
Paux
Etienne
E
Institut National de la Recherche Agronomique, Université Blaise Pascal (INRA-UBP), UMR 1095, Genetics Diversity and Ecophysiology of Cereals, Clermont-Ferrand, France.
Sourdille
Pierre
P
Salse
Jérôme
J
Saintenac
Cyrille
C
Choulet
Frédéric
F
Leroy
Philippe
P
Korol
Abraham
A
Michalak
Monika
M
Kianian
Shahryar
S
Spielmeyer
Wolfgang
W
Lagudah
Evans
E
Somers
Daryl
D
Kilian
Andrzej
A
Alaux
Michael
M
Vautrin
Sonia
S
Bergès
Hélène
H
Eversole
Kellye
K
Appels
Rudi
R
Safar
Jan
J
Simkova
Hana
H
Dolezel
Jaroslav
J
Bernard
Michel
M
Feuillet
Catherine
C
D001939:Bread; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics*; D020451:Contig Mapping; D016172:DNA Fingerprinting; D020224:Expressed Sequence Tags; D015723:Gene Library; D018745:Genome, Plant; D018895:Microsatellite Repeats; D012275:Oryza / Q000235:genetics; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic; D011123:Polyploidy; D021101:Radiation Hybrid Mapping; D014908:Triticum / Q000235:genetics*