The function of rhamnose-binding lectin in innate immunity by restricted binding to Gb3.
L-rhamnose-binding lectins (RBLs) have been isolated from various kinds of fish and invertebrates and interact with various kinds of bacteria, suggesting RBLs are involved in various inflammatory reactions. We investigated the effect of RBLs from chum salmon (Oncorhynchus keta), named CSL1, 2 and 3, on the peritoneal macrophage cell line from rainbow trout (Oncorhynchus mykiss) (RTM5) and an established fibroblastic-like cell line derived from gonadal tissue of rainbow trout (RTG-2). CSLs were bound to the surface of RTM5 and RTG-2 cells and induced proinflammatory cytokines, including IL-1beta1, IL-1beta2, TNF-alpha1, TNF-alpha2 and IL-8 in both cells by recognizing globotriaosylceramide (Gb3). In addition, CSLs had an opsonic effect on RTM5 cells and this effect was significantly inhibited by L-rhamnose, indicating that CSLs enhanced their phagocytosis by binding to Gb3 on cell surfaces. This is the first finding that Gb3 plays a role in innate immunity by cooperating with natural ligands, RBLs.
Watanabe
Yasuharu
Y
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
Tateno
Hiroaki
H
Nakamura-Tsuruta
Sachiko
S
Kominami
Junko
J
Hirabayashi
Jun
J
Nakamura
Osamu
O
Watanabe
Tasuku
T
Kamiya
Hisao
H
Naganuma
Takako
T
Ogawa
Tomohisa
T
Naudé
Ryno J
RJ
Muramoto
Koji
K
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects* / Q000276:immunology*; D037102:Lectins / Q000378:metabolism* / Q000494:pharmacology*; D017685:Oncorhynchus keta / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010586:Phagocytes; D011485:Protein Binding; D012210:Rhamnose / Q000378:metabolism*; D013379:Substrate Specificity
D000906:Antibodies; D015295:Antigens, Tumor-Associated, Carbohydrate; D016207:Cytokines; C472766:Gb3 antigen; D037102:Lectins; D012210:Rhamnose