A near-infrared fluorescent probe for lipid hydroperoxides in living cells.
An NIR (near-infrared) fluorescent probe TCP (tricarbocyanine diphenylphosphine) including a non-conjugated 'pre-tricarbocyanine' was designed and synthesized for visualizing lipid hydroperoxides (ROOH) in living cells. The excitation and emission spectra of tricarbocyanine in the NIR region can effectively avoid background fluorescence interference in biological systems. The probe exhibited a rapid fluorescence response to ROOH and high selectivity for ROOH over other ROS (reactive oxygen species) and some biological compounds, and the limit of detection was 38 pM. In addition, the probe was stable, and less cytotoxic, which indicated that it has potential application in detecting lipid hydroperoxides in living biological systems.
Li
Ping
P
College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Shandong Normal University, Jinan, PR China.
Tang
Bo
B
Xing
Yanlong
Y
Li
Puming
P
Yang
Guiwen
G
Zhang
Liang
L
D002232:Carbocyanines; D002477:Cells / Q000737:chemistry*; D005456:Fluorescent Dyes; D006801:Humans; D008054:Lipid Peroxides / Q000032:analysis*; D018613:Microscopy, Confocal; D010720:Phosphines; D017382:Reactive Oxygen Species / Q000032:analysis; D019265:Spectroscopy, Near-Infrared / Q000379:methods
D002232:Carbocyanines; D005456:Fluorescent Dyes; D008054:Lipid Peroxides; D010720:Phosphines; D017382:Reactive Oxygen Species