[Study on quality evaluation of Radix Isatis based on analysis of biothermic activity].
OBJECTIVE
To establish a novel pattern and method to evaluate the quality of Radix Isatis based on analysis of biothermic activity.


METHODS
Chemical and biothermodynamic methods were used and compared to investigate the quality of different Radix Isatis samples. And a mathematic model was established by computer aided pattern recognition to evaluate the quality of Radix Isatis.


RESULTS
The quality of Radix Isatis was partially related to the content of organic acids and polycose, but it could not be correctly recognized by both chemical determination and HPLC fingerprint. On the other hand, the mathematic model based on the main four parameters of biothermodynamic analysis was very correct (misjudgment ratio of 1.39%) to recognize the quality of Radix Isatis.


CONCLUSIONS
The established model in this paper based on analysis of biothermic activity is more accurate and reliable than that of chemical methods to evaluate the quality of Radix Isatis.
Zhao
Yan-Ling
YL
PLA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039, China.
Shan
Li-Mei
LM
Jin
Cheng
C
Zhou
Xu
X
Xiao
Xiao-He
XH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002151:Calorimetry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D031219:Isatis / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008962:Models, Theoretical; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000592:standards; D011786:Quality Control; D015203:Reproducibility of Results; D013816:Thermodynamics
D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides