Differential invasion of Trypanosoma brucei brucei and lymphocytes into the brain of C57BL/6 and 129Sv/Ev mice.
Trypanosoma brucei subspecies invade the brain parenchyma at late stages of human and experimental rodent infections. In this study, we compared the outcome of infection with T. b. brucei in MHC-matched (H-2b) C57BL/6 (B6) and 129Sv/Ev (Sv-129). Sv-129 showed higher parasitaemia and lower specific IgM (but not IgG) antibody levels than B6 mice. The number of trypanosomes, CD4+ and CD8+ T cells in the brain parenchyma was higher in B6 mice. B6 mice lost weight and showed higher cumulative mortality when compared with Sv-129 mice. Higher levels of IL-1beta, IL-6, IL-10, TNF-alpha, IFN-gamma, ICAM-1 and E-selectin, but low levels of TGF-beta mRNA were present in brains of B6 when compared with Sv-129-infected mice. Thus, host genetics differentially determine the invasion of T. b. brucei into the brain parenchyma, which is paralleled by the severity of inflammation in the brain and course of the disease, but not by parasitaemia nor by antibody titres.
Masocha
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W
Department of Applied Therapeutics, Faculty of Pharmacy, Kuwait University, Safat, Kuwait. masocha@hsc.edu.kw
Amin
D N
DN
Kristensson
K
K
Rottenberg
M E
ME
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001927:Brain Diseases / Q000276:immunology* / Q000469:parasitology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D005904:Glial Fibrillary Acidic Protein; D006649:Histocompatibility Antigens / Q000276:immunology; D054884:Host-Pathogen Interactions; D007150:Immunohistochemistry; D016177:Macrophage-1 Antigen / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D018512:Parasitemia / Q000276:immunology / Q000469:parasitology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014346:Trypanosoma brucei brucei / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000235:genetics / Q000276:immunology* / Q000469:parasitology
D000913:Antibodies, Protozoan; D015815:Cell Adhesion Molecules; D016207:Cytokines; D005904:Glial Fibrillary Acidic Protein; D006649:Histocompatibility Antigens; D016177:Macrophage-1 Antigen; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C528900:glial fibrillary astrocytic protein, mouse