Recognition of phospho sugars and nucleotides with an array of boronic acid appended bipyridinium salts.
The solution-phase sensor array of three cationic bis-boronic acid appended benzyl viologens (BBV) and the anionic fluorescent dye, 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS), is able to discriminate among five phospho sugars, four nucleotides and three neutral saccharides in aqueous buffered solution at low mM concentrations. Linear discriminant analysis, principal component analysis, and hierachical cluster analysis studies showed the "discrimination limit" (lowest analyte concentration where the discrimination is still 100%) to be 4mM. Calculated K(b) and F(max)/F(0) values from binding curves of the three BBVs with 1-12 were also used to perform multi-variate analyses with very good discrimination results.
Schiller
Alexander
A
Empa - Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Protection and Physiology, Lerchenfeldstr. 5, 9014 St. Gallen, Switzerland.
Vilozny
Boaz
B
Wessling
Ritchie A
RA
Singaram
Bakthan
B
D001190:Arylsulfonates / Q000737:chemistry*; D015374:Biosensing Techniques; D001897:Boronic Acids / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D009711:Nucleotides / Q000032:analysis* / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013403:Sugar Phosphates / Q000032:analysis* / Q000737:chemistry; D014755:Viologens / Q000737:chemistry*
D001190:Arylsulfonates; D001897:Boronic Acids; D005456:Fluorescent Dyes; D009711:Nucleotides; D012996:Solutions; D013403:Sugar Phosphates; D014755:Viologens; C005047:pyranine