[Optimization for extraction technology of polysaccharide in root of Adenophora potaninii from Taibai mountain in China by orthogonal experimental design].
OBJECTIVE
This study is to develop excellent extraction technology of the polysaccharides in the root of the A. potaninii which live in the Taibai Mountain in China.


METHODS
Based on the extraction with water, the polysaccharides were deposited with alcohol. With the content of polysaccharides was as the index, extraction conditions were investigated systemly. Employed the solid-liquid ratio, extraction temperature, extraction time and extraction number of times were as levels of single factor, the optimal extraction technology of the polysaccharides from the root of the A. potaninii was determined by L9 (3(4)) orthogonal experimental design L9 (3(4)).


RESULTS
The optimal technology conditions were that the solid-liquid ratio was 1 : 30, the extracting temperature was 60 degrees C, the extracting time was 3 h and extracting number of times was 3 times.


CONCLUSIONS
The optimized extraction technology is simple, reliable and extraction efficiency of polysaccharide is higher.
Li
Peng-Cheng
PC
College of Chemistry and Material Science, Shanxi Normal University, Xi'an 710062, China.
Zhang
Ben-Cai
BC
Chen
Zhan-Guo
ZG
D029422:Campanulaceae / Q000737:chemistry*; D002681:China; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D014867:Water