[The influence of lysozyme dimmer on chosen parameters of blood in healthy rabbits].
The natural compounds which affect defensive mechanisms of organism are important in prophylaxis and therapy of diseases in human and animals. Lysozyme is an enzyme which originates from chicken egg proteins. It modulates non-specific and humoral immunological mechanisms. A higher bioactivity has a lysozyme dimmer. The aim of study was assessment of influence of the lysozyme dimmer on chosen blood parameters, morphology and functions of internal organs and healing processes of experimental wounds in rabbits. Lysozyme dimmer (KLP-602) was used obtained process polymerisations enzyme lyzosyme from chicken egg white. The experimental group were on New Zealand White rabbits. Systemic reactions were investigated in animals after two injections of lysozyme dimer in dose 0,02 mg/kg b.w. during 21 days. Blood was collected before and after administration of lysozyme dimer in 4, 6, 24 h and in 3, 7, 21 day after first and in 4, 24 h and 3, 7 day after second injection. The following parameters were evaluated of red blood cells number (RBC), hemoglobin concentration (HGB), haematocrit value (HCT), red blood cell indices (MCV, MCH, MCHC), white blood cells number (WBC) and leukogram. Total serum proteins, components C3 and C4 of complement, immunoglobulins G and M, concentration were determined in the serum. Activated partial thromboplastin time (APTT), prothrombin time (PT) and fibrinogen concentration were evaluated in the plasma. Simultaneously, the activity of alkaline phosphatate, GPT and GOT were assessed. Administration rabbits with lysozyme dimmer caused slight decrease in RBC number, Hb concentration, HCT and the neutrophils percentage and increase in the lymphocytes percentage. The concentrations of TSP, immunoglobulins, the components of complement ware increased too. APTT an PT were normal but the fibrinogen concentration was increased. The activity of GOT and GPT were unchanged. Changes are in range of value normal and step out more quickly after two injection than after firste injection. The morphology of organs (liver, lungs, heart and kidneys) was normal.
Szymonowicz
Maria
M
Zakład Chirurgii Eksperymentalnej i Badania Biomaterial'ow6w Wydzial Lekarsko-Stomatologiczny Akademii Medycznej we Wrocławiu. biochem@cheksp.am.wroc.pl
D000818:Animals; D004906:Erythrocyte Count; D005260:Female; D006454:Hemoglobins / Q000187:drug effects; D007958:Leukocyte Count; D018655:Lymphocyte Count; D008297:Male; D009113:Muramidase / Q000378:metabolism* / Q000494:pharmacology; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects* / Q000276:immunology / Q000502:physiology*
D006454:Hemoglobins; C488571:KLP602; D009113:Muramidase