title: Microarray-based genomic profiling reveals novel genomic aberrations in follicular lymphoma which associate with patient survival and gene expression status.
abstract: Follicular lymphoma (FL) is characterized by a large number of chromosomal aberrations. However, their exact genomic extension and involved target genes remain to be determined. For this purpose, we used array-based intermediate-high resolution genomic profiling in combination with Affymetrix gene expression analysis. Tumor specimens from 128 FL patients were analyzed for the presence of genomic aberrations and the results were correlated to clinical data sets and mRNA expression levels. In 114 (89%) of the 128 analyzed cases, a total of 688 genomic aberrations (384 gains/amplifications and 304 losses) were detected. Frequent genomic aberrations were: -1p36 (18%), +2p15 (24%), -3q (14%), -6q (25%), +7p (19%), +7q (23%), +8q (14%), -9p (16%), -11q (15%), +12q (20%), -13q (11%), -17p (16%), +18p (18%), and +18q (28%). Critical segments of these imbalances were delineated to genomic fragments with a minimum size down to 0.2 Mb. By comparison of these with mRNA gene expression data, putative candidate genes were identified. Moreover, we found that deletions affecting the tumor suppressor gene CDKN2A/B on 9p21 were detected in nontransformed FL grade I-II. For this aberration as well as for -6q25 and -6q26, an association with inferior survival was observed.
authors.affiliation: Abt Innere Medizin III, Zentrum für Innere Medizin der Universität Ulm, Ulm, Germany. c.schwaenen@klinikum-esslingen.de
authors.forename: Carsten
authors.initials: C
authors.lastname: Schwaenen
authors.forename: Andreas
authors.initials: A
authors.lastname: Viardot
authors.forename: Hilmar
authors.initials: H
authors.lastname: Berger
authors.forename: Thomas F E
authors.initials: TF
authors.lastname: Barth
authors.forename: Stefan
authors.initials: S
authors.lastname: Bentink
authors.forename: Hartmut
authors.initials: H
authors.lastname: Döhner
authors.forename: Martina
authors.initials: M
authors.lastname: Enz
authors.forename: Alfred C
authors.initials: AC
authors.lastname: Feller
authors.forename: Martin-Leo
authors.initials: ML
authors.lastname: Hansmann
authors.forename: Michael
authors.initials: M
authors.lastname: Hummel
authors.forename: Hans A
authors.initials: HA
authors.lastname: Kestler
authors.forename: Wolfram
authors.initials: W
authors.lastname: Klapper
authors.forename: Markus
authors.initials: M
authors.lastname: Kreuz
authors.forename: Dido
authors.initials: D
authors.lastname: Lenze
authors.forename: Markus
authors.initials: M
authors.lastname: Loeffler
authors.forename: Peter
authors.initials: P
authors.lastname: Möller
authors.forename: Hans-Konrad
authors.initials: HK
authors.lastname: Müller-Hermelink
authors.forename: German
authors.initials: G
authors.lastname: Ott
authors.forename: Maciej
authors.initials: M
authors.lastname: Rosolowski
authors.forename: Andreas
authors.initials: A
authors.lastname: Rosenwald
authors.forename: Sandra
authors.initials: S
authors.lastname: Ruf
authors.forename: Reiner
authors.initials: R
authors.lastname: Siebert
authors.forename: Rainer
authors.initials: R
authors.lastname: Spang
authors.forename: Harald
authors.initials: H
authors.lastname: Stein
authors.forename: Lorenz
authors.initials: L
authors.lastname: Truemper
authors.forename: Peter
authors.initials: P
authors.lastname: Lichter
authors.forename: Martin
authors.initials: M
authors.lastname: Bentz
authors.forename: Swen
authors.initials: S
authors.lastname: Wessendorf
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D002648:Child; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D053208:Kaplan-Meier Estimate; D008224:Lymphoma, Follicular / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D055815:Young Adult