title: Structural variants of biodegradable polyesterurethane in vivo evoke a cellular and angiogenic response that is dictated by architecture.
abstract: The aim of this study was to investigate an in vivo tissue response to a biodegradable polyesterurethane, specifically the cellular and angiogenic response evoked by varying implant architectures in a subcutaneous rabbit implant model. A synthetic biodegradable polyesterurethane was synthesized and processed into three different configurations: a non-porous film, a porous mesh and a porous membrane. Glutaraldehyde cross-linked bovine pericardium was used as a control. Sterile polyesterurethane and control samples were implanted subcutaneously in six rabbits (n=12). The rabbits were killed at 21 and 63 days and the implant sites were sectioned and histologically stained using haemotoxylin and eosin (H&E), Masson's trichrome, picosirius red and immunostain CD31. The tissue-implant interface thickness was measured from the H&E slides. Stereological techniques were used to quantify the tissue reaction at each time point that included volume fraction of inflammatory cells, fibroblasts, fibrocytes, collagen and the degree of vascularization. Stereological analysis inferred that porous scaffolds with regular topography are better tolerated in vivo compared to non-porous scaffolds, while increasing scaffold porosity promotes angiogenesis and cellular infiltration. The results suggest that this biodegradable polyesterurethane is better tolerated in vivo than the control and that structural variants of biodegradable polyesterurethane in vivo evoke a cellular and angiogenic response that is dictated by architecture.
authors.affiliation: Department of Mechanical and Biomedical Engineering, National University of Ireland, Galway, University Road, Galway, Ireland.
authors.forename: Jerome A
authors.initials: JA
authors.lastname: Henry
authors.forename: Krishna
authors.initials: K
authors.lastname: Burugapalli
authors.forename: Peter
authors.initials: P
authors.lastname: Neuenschwander
authors.forename: Abhay
authors.initials: A
authors.lastname: Pandit
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D003094:Collagen / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry; D008297:Male; D008954:Models, Biological; D009389:Neovascularization, Pathologic; D010496:Pericardium / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D011091:Polyesters / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011091:Polyesters; D011140:Polyurethanes; D003094:Collagen; D005976:Glutaral