title: Quantification of sunitinib in human plasma by high-performance liquid chromatography-tandem mass spectrometry.
abstract: A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sunitinib in human plasma. Sample preparation involved a liquid-liquid extraction by the addition of 0.2mL of plasma with 4.0mL tert-butyl-methyl-ether extraction solution containing 25ng/mL of the internal standard clozapine. Separation of compounds was achieved on a C18 (50mmx2.1mm i.d., 3.5microm) analytical column using a mobile phase consisting of acetonitrile/H20 (65:35, v/v) containing 0.1% formic acid and isocratic flow at 0.150mL/min for 3min. The analytes were monitored by tandem-mass spectrometry with electrospray positive ionization. Linear calibration curves in human plasma were generated over the range of 0.2-500ng/mL with values for the coefficient of determination of >0.9950. Within- and between day precision and accuracy were < or =10%. The method was applied to the quantitation of sunitinib in plasma samples from a patient receiving daily oral therapy with sunitinib.
authors.affiliation: Pharmaceutical Sciences Department, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors.forename: Patton
authors.initials: P
authors.lastname: Minkin
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Zhaoyuan
authors.initials: Z
authors.lastname: Chen
authors.forename: Jan
authors.initials: J
authors.lastname: Ouwerkerk
authors.forename: Hans
authors.initials: H
authors.lastname: Gelderblom
authors.forename: Sharyn D
authors.initials: SD
authors.lastname: Baker
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007211:Indoles / Q000097:blood* / Q000737:chemistry; D015394:Molecular Structure; D011758:Pyrroles / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D000077210:Sunitinib; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007211:Indoles; D011758:Pyrroles; D000077210:Sunitinib