title: Mechanisms of myocyte cytotoxicity induced by the multiple receptor tyrosine kinase inhibitor sunitinib.
abstract: The anticancer tyrosine kinase inhibitor sunitinib has been shown recently to be cardiotoxic. Using a neonatal rat myocyte model, we investigated various mechanisms that might be responsible for its cardiotoxicity. Sunitinib potently inhibited the enzyme activity of both AMP-activated protein kinase (AMPK) and the ribosomal S6 kinase RSK1 at therapeutically relevant concentrations. Heart tissue with its high energy needs might be particularly sensitive to inhibition of AMPK because of its role as an energy sensor regulating ATP levels. As measured by lactate dehydrogenase release, sunitinib treatment of myocytes caused dose-dependent damage at therapeutic levels. Sunitinib treatment also caused a dose-dependent reduction in myocyte protein levels of the phosphorylated alpha and beta isoforms of the AMPK phosphorylation target acetyl-Coenzyme A carboxylase. However, myocytes were not protected from sunitinib treatment by pretreating them with the AMPK-activating antidiabetic drug metformin. Sunitinib treatment of myocytes also did not affect cellular ATP levels. Together, these last two results do not suggest a major role for inhibition of AMPK in sunitinib-induced myocyte damage. Dexrazoxane, which is a clinically approved doxorubicin cardioprotective agent, also did not protect myocytes from damage, which suggests that sunitinib did not induce oxidative damage. In conclusion, even though sunitinib potently inhibits AMPK and RSK1, given the extreme lack of kinase selectivity that sunitinib exhibits, it is likely that inhibition of other kinases or combinations of kinases are responsible for the cardiotoxic effects of sunitinib.
authors.affiliation: Faculty of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, Manitoba, Canada. b_hasinoff@umanitoba.ca
authors.forename: Brian B
authors.initials: BB
authors.lastname: Hasinoff
authors.forename: Daywin
authors.initials: D
authors.lastname: Patel
authors.forename: Kimberley A
authors.initials: KA
authors.lastname: O'Hara
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D000831:Animals, Newborn; D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis; D002478:Cells, Cultured; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007211:Indoles / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008687:Metformin / Q000494:pharmacology; D032342:Muscle Cells / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress; D010629:Phenformin / Q000494:pharmacology; D010766:Phosphorylation; D011758:Pyrroles / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020794:Receptor Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D038744:Ribosomal Protein S6 Kinases, 90-kDa / Q000037:antagonists & inhibitors; D000077210:Sunitinib
chemical_list: D000970:Antineoplastic Agents; D007004:Hypoglycemic Agents; D007211:Indoles; D011758:Pyrroles; D000255:Adenosine Triphosphate; D008687:Metformin; D010629:Phenformin; D007770:L-Lactate Dehydrogenase; D020794:Receptor Protein-Tyrosine Kinases; D038744:Ribosomal Protein S6 Kinases, 90-kDa; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase; D000077210:Sunitinib