[Analysis of expressed sequence tags (EST) from Panax quinquefolium root].
To investigate the profile of gene expression in American ginseng (Panax quinquefolium L.) and discover its functional genes, for the first time, expressed sequence tags (EST) library of four-year-old American ginseng roots has been established. According to BLAST and Gene Ontology analysis, eleven genes, encoding cytochrome P450, glucosyltransferase, farnesyltransferase and cyclase family protein, are found to be associated with ginsenosides biosynthesis. Six other genes are obtained encoding auxin-regulated protein, auxin response factor 4 and auxin-repressed protein in the roots of American ginseng. In addition, thirteen expressed transcripts are stress-connected proteins and twelve expressed other transcripts are closely related to plant defense in four-year-old American ginseng roots. Furthermore, 62 genes no hit in BLAST and in Interproscan may be new genes. These results indicate EST is an useful tool for research on functional genomics of P. quinquefolium and it can be applied to the molecular modification of the ginsenosides biosynthetic pathway ultimately for improving the quality of American ginseng germplasm.
Chen
Shi-Lin
SL
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100094, China.
Sun
Yong-Qiao
YQ
Song
Jing-Yuan
JY
Li
Ying
Y
Li
Chen-Ji
CJ
Hu
Song-Nian
SN
Li
Xi-Wen
XW
Yao
Hui
H
Zhang
Xiao-Wei
XW
D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D017343:Genes, Plant; D036145:Ginsenosides / Q000096:biosynthesis; D005894:Panax / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics
D018744:DNA, Plant; D036145:Ginsenosides