Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I.
Sybr Green I efficiently discriminates mercury-specific DNA and mercury-specific DNA/Hg(2+) complex, which provides a label-free, fast, fluorescence turn on assay for Hg(2+) detection with high sensitivity and selectivity.
Wang
Jing
J
Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117576.
Liu
Bin
B
D052160:Benzothiazoles; D015374:Biosensing Techniques; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D003959:Diamines; D004566:Electrodes; D005453:Fluorescence; D008628:Mercury / Q000032:analysis*; D009930:Organic Chemicals / Q000737:chemistry*; D011804:Quinolines; D012996:Solutions / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D014867:Water / Q000737:chemistry
D052160:Benzothiazoles; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; D012996:Solutions; D014867:Water; C098022:SYBR Green I; D004247:DNA; D008628:Mercury