Polyaniline-carbon nanotube composite film for cholesterol biosensor.
Nanocomposite film composed of polyaniline (PANI) and multiwalled carbon nanotubes (MWCNT), prepared electrophoretically onto indium tin oxide (ITO)-coated glass plate, was used for covalent immobilization of cholesterol oxidase (ChOx) via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry. Results of linear sweep voltammetric measurements reveal that ChOx/PANI-MWCNT/ITO bioelectrode can detect cholesterol in the range of 1.29 to 12.93 mM with high sensitivity of 6800 nA mM(-1) and a fast response time of 10 s. Photometric studies for ChOx/PANI-MWCNT/ITO bioelectrode indicate that it is thermally stable up to 45 degrees C and has a shelf life of approximately 12 weeks when stored at 4 degrees C. The results of these studies have implications for the application of this interesting matrix (PANI-MWCNT) toward the development of other biosensors.
Dhand
Chetna
C
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India.
Arya
Sunil K
SK
Datta
Monika
M
Malhotra
B D
BD
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002784:Cholesterol / Q000032:analysis* / Q000378:metabolism; D002789:Cholesterol Oxidase / Q000378:metabolism; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D004586:Electrophoresis; D005898:Glass / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011551:Pseudomonas fluorescens / Q000201:enzymology; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D017971:Tin Compounds / Q000737:chemistry
D000814:Aniline Compounds; D037742:Nanotubes, Carbon; D017971:Tin Compounds; C416807:polyaniline; C109984:indium tin oxide; D002784:Cholesterol; D002789:Cholesterol Oxidase