Microbial community in the rhizosphere of young maize seedlings is susceptible to the impact of introduced pseudomonads as indicated by FAME analysis.
Two species of Pseudomonas (i.e. P. chlororaphis or P. putida) derived from a maize rhizosphere were studied for their impact on the structure of the microbial community in the rhizosphere of young maize seedlings after inoculation. The culturable bacteria and total microbial communities were analyzed based on profiles of whole-cell fatty acid methyl esters (MIDI-FAME). The introduction of Pseudomonas species resulted in the shift from the Gram-positive dominated culturable community in the rhizosphere of uninoculated maize to more Gram-negative populations in the rhizospheres of the inoculated plants. For the total rhizosphere communities, 43, 47 and 42 FAMEs were detected in the uninoculated maize and the samples inoculated with P. chlororaphis or P. putida, respectively. In contrast to the culturable communities, low concentrations of marker FAMEs for Gram-positives (i15:0, a15:0, i16:0) were found in the profiles of the total rhizosphere communities. The maize inoculations resulted in an enrichment of some Gram-negative isolates; however, Gram-positive bacteria, Cytophaga/Flavobacterium and saprophytic fungi were found in the uninoculated rhizosphere.
Kozdrój
Jacek
J
Institute of Engineering and Environmental Protection, University of Bielsko-Biala, Bielsko-Biala, Poland. jkozdroj@ath.bielsko.pl
D018556:Crops, Agricultural / Q000382:microbiology; D017753:Ecosystem; D005227:Fatty Acids / Q000737:chemistry; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000382:microbiology*; D016958:Pseudomonas putida / Q000737:chemistry / Q000254:growth & development*; D036226:Seedlings / Q000382:microbiology*; D012988:Soil Microbiology; D003313:Zea mays / Q000382:microbiology*
D005227:Fatty Acids