title: Expression and polimorphism of IFN-gamma gene in patients with cervical cancer.
abstract: BACKGROUND
  Cervical cancer, the second most common malignancy in women worldwide, is almost invariably associated with infection by human papillomavirus (HPV). However, although many women are infected with high-risk types of HPV, only a subset of infected women will ever develop cervical cancer. Several studies suggested that immunological components play a key role in the development of cervical cancer. Interferon gamma (IFN-gamma ) is a cytokine produced by activated T cells and natural killer (NK) cells that enhances cellular immune responses by increasing T-cell cytotoxicity and NK cell activity.
  
  
  OBJECTIVE
  To study single nucleotide polymorphism (SNP), T to A, located at the +874 position and measure IFN-gamma messenger RNA (mRNA) at the tumor site.
  
  
  METHODS
  DNA was isolated from peripheral blood of 200 patients with cervical cancer and 200 healthy controls. The allele polymorphism at position +874 in the IFN-gamma gene was studied by ARMS-PCR (Amplification Refractory Mutation System) and measured IFN-gamma mRNA at the tumor site by means of a semi-quantitative polymerase chain reaction (sqRT-PCR) assay.
  
  
  RESULTS
  It was observed that genotypes AT and AA + AT increase the risk of cervical cancer (OR = 3.3, 95% CI - 2.05-5.2, P <or= 0.001 - OR = 2.9, 95% CI - 1.9-4.6, P <or= 0.001, respectively). In case of passive smokers same genotypes showed highly significant increased risk of cervical cancer (OR = 5.55, 95% CI = 2.77-11.19 - OR = 5.25, 95% CI = 2.77-10, respectively). Thus, the sqRT-PCR reflected the similar level of mRNA expression of IFN- gamma gene in patients suffering from cervical carcinoma and healthy controls.
  
  
  CONCLUSIONS
  This is the first study to provide an evidence for effecting of IFN-gamma gene on the risk of cervical cancer in north Indian population.
authors.affiliation: Department of Biotechnology, Panjab University, Chandigrah, India. dor_kordi@yahoo.com
authors.forename: M K
authors.initials: MK
authors.lastname: Kordi Tamandani
authors.forename: R C
authors.initials: RC
authors.lastname: Sobti
authors.forename: M
authors.initials: M
authors.lastname: Shekari
authors.forename: M
authors.initials: M
authors.lastname: Mukesh
authors.forename: V
authors.initials: V
authors.lastname: Suri
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000556:secondary; D000293:Adolescent; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000556:secondary; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D007371:Interferon-gamma