title: Detergent-associated solution conformations of helical and beta-barrel membrane proteins.
abstract: Membrane proteins present major challenges for structural biology. In particular, the production of suitable crystals for high-resolution structural determination continues to be a significant roadblock for developing an atomic-level understanding of these vital cellular systems. The use of detergents for extracting membrane proteins from the native membrane for either crystallization or reconstitution into model lipid membranes for further study is assumed to leave the protein with the proper fold with a belt of detergent encompassing the membrane-spanning segments of the structure. Small-angle X-ray scattering was used to probe the detergent-associated solution conformations of three membrane proteins, namely bacteriorhodopsin (BR), the Ste2p G-protein coupled receptor from Saccharomyces cerevisiae, and the Escherichia coli porin OmpF. The results demonstrate that, contrary to the traditional model of a detergent-associated membrane protein, the helical proteins BR and Ste2p are not in the expected, compact conformation and associated with detergent micelles, while the beta-barrel OmpF is indeed embedded in a disk-like micelle in a properly folded state. The comparison provided by the BR and Ste2p, both members of the 7TM family of helical membrane proteins, further suggests that the interhelical interactions between the transmembrane helices of the two proteins differ, such that BR, like other rhodopsins, can properly refold to crystallize, while Ste2p continues to prove resistant to crystallization from an initially detergent-associated state.
authors.affiliation: Center for Structural Molecular Biology, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Mo
authors.forename: Byung-Kwon
authors.initials: BK
authors.lastname: Lee
authors.forename: John F
authors.initials: JF
authors.lastname: Ankner
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Becker
authors.forename: William T
authors.initials: WT
authors.lastname: Heller
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry; D002942:Circular Dichroism; D003902:Detergents / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D018272:Porins / Q000737:chemistry; D017433:Protein Structure, Secondary; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D053838:Scattering, Small Angle; D012996:Solutions; D014961:X-Ray Diffraction
chemical_list: D003902:Detergents; D008565:Membrane Proteins; C109237:OmpF protein; D018272:Porins; D043562:Receptors, G-Protein-Coupled; D029701:Saccharomyces cerevisiae Proteins; D012996:Solutions; D001436:Bacteriorhodopsins