On the measurement of the functional properties of the CFTR.
A number of methods are currently employed to assess the functional properties of CFTR channels and their response to pharmacological potentiators, correction of the defective CFTR trafficking, and vectorial introduction of new proteins. Here we review the most common methods used to assess CFTR channel function. The suitability of each technique to various experimental conditions is discussed.
Moran
Oscar
O
Istituto di Biofisica, CNR, 16149 Genova, Italy. moran@ge.ibf.cnr.it
Zegarra-Moran
Olga
O
D003550:Cystic Fibrosis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000502:physiology*; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology
C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator