Nuclear matrix contains novel WD-repeat and disordered-region-rich proteins.
To find novel proteins predicted to participate in the formation of nuclear bodies, nuclear speckles, and nuclear macro-protein complexes, we applied proteome analysis to a HeLa cell nuclear matrix fraction. Proteins in the fraction were separated by SDS-PAGE, digested with trypsin, and analyzed by nanoflow liquid chromatography-iontrap-tandem mass spectrometry. Three hundred and thirty three proteins including 39 novel ones were identified. Seven WD-repeat proteins and 16 disordered region-rich proteins, which act frequently as scaffolding proteins for macro-protein complexes, were found amongst the novel proteins.
Ishii
Kohei
K
Graduate School of Science and Technology, Niigata University, Igarashi-2, Nishi-ku, Niigata 950-2181, Japan.
Hirano
Yasuhiro
Y
Araki
Nanase
N
Oda
Toshiyuki
T
Kumeta
Masahiro
M
Takeyasu
Kunio
K
Furukawa
Kazuhiro
K
Horigome
Tsuneyoshi
T
D006367:HeLa Cells; D006801:Humans; D015530:Nuclear Matrix / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D020543:Proteome; D020449:Repetitive Sequences, Amino Acid
D009687:Nuclear Proteins; D020543:Proteome