[Production of ethanol and isoflavones from steam-pretreated Radix Puerariae by solid state fermentation].
The gelatinization process of the starch is replaced by unpolluted steam-pretreatment on the base of the Radix Puerariae rich in fiber and isoflavones. The production of ethanol and isoflavones by simultaneous saccharification and solid state fermentation (SSF) of steam-pretreatment Radix Puerariae is presented. The optimal technological conditions were obtained: Radix Puerariae being steam-pretreated at a saturated vapor pressure of 0.8 MPa for 3.5 min, glucoamylase(65 u/g), cellulase(1.5 u/g), 0.1%(NH4)2SO4, 0.1%KH2PO4 and activated yeasts being added in, and fermentation at 35-37 degrees C for 60 h. Under these conditions, the yield of ethanol and isoflavones from 100 g Radix Pureriae (dry basis) were 27.47 g and 4.43 g, respectively, the starch utilization rate was 95%. In comparison with the traditional fermentation technology, the simultaneous saccharification and SSF of steam-pretreatment Radix Puerariae is clean and energy-saving. It provides new way of the production of ethanol from the non-food starch material, and worthwhile to be explored and implemented in industry.
Fu
Xiaoguo
X
Key State Laboratory of Biochemical Engineering Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Chen
Hongzhang
H
Wang
Weidong
W
D002480:Cellulase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology*; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D007529:Isoflavones / Q000096:biosynthesis*; D029908:Pueraria / Q000378:metabolism*; D013227:Steam; D015003:Yeasts / Q000378:metabolism*
D007529:Isoflavones; D013227:Steam; D000431:Ethanol; D005087:Glucan 1,4-alpha-Glucosidase; D002480:Cellulase