title: A pilot study on the distribution of human papillomavirus genotypes and HPV-16 variants in cervical neoplastic lesions from Ecuadorian women.
abstract: Human papillomaviruses (HPVs) are the cause of cervical intraepithelial neoplasia and invasive carcinomas of the uterine cervix. The distribution of specific HPV genotypes varies greatly across populations and HPV surveys have been performed in different geographical regions in order to apply appropriate vaccine strategies. The aim of this study was to determine the spectrum of HPV genotypes and HPV-16 variants among women with cervical lesions living in Ecuador. A total of 71 cases have been analyzed, including 32 chronic cervicitis, 29 cervical intraepithelial neoplasia grade 1, and 10 cervical intraepithelial neoplasia grade 2-3. HPV sequences were detected by broad spectrum consensus-primer-pairs MY09/MY11 and GP5+/GP6+-based polymerase chain reaction and characterized by nucleotide sequence analysis. Overall, 31 (43.7%) cases were HPV positive with prevalence rates of 37.5%, 44.8%, and 60% in patients with chronic cervicitis, cervical intraepithelial neoplasia grade 1 and cervical intraepithelial neoplasia grade 2-3, respectively. Among the positive cases, the most common genotypes were HPV 16 (64.5%) and HPV 81 (29%) followed by HPV 31, 53, 56, and 58, in descending order of prevalence. Seventeen (85%) HPV-16 isolates were classified as European and three (15%) as African-1 variant on the basis of nucleotide signature present within the MY09/MY11 L1 sequence. The results suggest that HPV 16 has a very high prevalence among women with cervical lesions in Ecuador; therefore, an effective HPV-16 based vaccine should prevent the development of cervical cancer in a large proportion of Ecuadorian women.
authors.affiliation: Molecular Biology and Viral Oncology and AIDS Reference Centre, National Cancer Institute, Fond. Pascale, Naples, Italy.
authors.forename: Maria Lina
authors.initials: ML
authors.lastname: Tornesello
authors.forename: Luigi
authors.initials: L
authors.lastname: Buonaguro
authors.forename: Sergio
authors.initials: S
authors.lastname: Izzo
authors.forename: Guillermo
authors.initials: G
authors.lastname: Lopez
authors.forename: Ximena
authors.initials: X
authors.lastname: Vega
authors.forename: Claudio Francisco
authors.initials: CF
authors.lastname: Maldonado Reyes
authors.forename: Franco Maria
authors.initials: FM
authors.lastname: Buonaguro
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004484:Ecuador / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D010802:Phylogeny; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D002583:Uterine Cervical Neoplasms / Q000821:virology*
chemical_list: D004279:DNA, Viral