title: Haematological changes in Nile tilapia experimentally infected with Enterococcus sp.
abstract: This study evaluated the haematological changes in Nile tilapia experimentally infected with 1 x 10(3) and 1 x 10(6) colony-forming units (CFU)/mL of Enterococcus sp. in the swim bladder. The experiment consisted of four treatments in triplicates: non-injected fish (NI); fish injected with 1 mL of sterile saline solution 0.65% (SAL); fish injected with 1 x 10(3) and 1 x 10(6) CFU/mL of Enterococcus diluted in 1 mL sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected. The haematological tests included red blood cell (RBC) and white blood cell (WBC) counts, hematocrit, number of total thrombocytes, and differential counting of WBC. Fish injected with 1 x 10(6) CFU/mL of Enterococcus showed a higher number of thrombocytes than the other treatments. White blood cell and lymphocyte numbers increased significantly in fish injected with 1 x 10(6) CFU/mL of Enterococcus when compared to non-injected control. There was significant increase in the number of neutrophils in saline injected fish and reduced number of monocytes after injections with 1 x 10(6) CFU/mL of Enterococcus. Hematocrit increased in fish injected with 1 x 10(3) and 1 x 10(6) CFU/mL of Enterococcus.
authors.affiliation: Departamento de Aqüicultura, Laboratório de Pesquisas em Sanidade de Organismos Aquáticos, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. mlaterca@cca.ufsc.br
authors.forename: M L
authors.initials: ML
authors.lastname: Martins
authors.forename: J L P
authors.initials: JL
authors.lastname: Mouriño
authors.forename: G V
authors.initials: GV
authors.lastname: Amaral
authors.forename: F N
authors.initials: FN
authors.lastname: Vieira
authors.forename: G
authors.initials: G
authors.lastname: Dotta
authors.forename: A M B
authors.initials: AM
authors.lastname: Jatobá
authors.forename: F S
authors.initials: FS
authors.lastname: Pedrotti
authors.forename: G T
authors.initials: GT
authors.lastname: Jerônimo
authors.forename: C C
authors.initials: CC
authors.lastname: Buglione-Neto
authors.forename: G
authors.initials: G
authors.lastname: Pereira
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D023681:Cichlids / Q000097:blood / Q000382:microbiology; D016983:Enterococcus; D016908:Gram-Positive Bacterial Infections / Q000097:blood / Q000382:microbiology