Nutrition, metabolism, and fertility in dairy cows: 2. Dietary fatty acids and ovarian function.
Plasma insulin has important implications for ovarian function in dairy cows. Previous work demonstrated that plasma insulin increased with increasing dietary starch and decreasing dietary fatty acid concentrations. The objective of this experiment was to investigate hormonal and ovarian responses to dietary fatty acid content with no change in other dietary components. Thirty cows were fed a standard diet from calving until 40 d in milk (DIM) and then 6 cows were transferred to each of 5 diets containing 0, 8, 15, 23, and 30 g/kg of dry matter (DM) of calcium salts of palm fatty acids (CaPFA; Megalac) until 70 DIM. Estrus was synchronized at 60 DIM. Between 60 and 70 DIM, energy intake, milk yield, and energy balance were similar among diet groups. Plasma insulin decreased when dietary concentration of CaPFA exceeded 15 g/kg of DM (insulin: 0.46, 0.41, 0.46, 0.33, 0.28 +/- SE 0.034 ng/mL for diets containing 0 to 30 g of CaPFA/kg of DM, respectively). Maximum plasma insulin to glucagon ratio was observed with 15 g of CaPFA/kg of DM (ratios: 3.99, 4.33, 4.67, 3.45, 2.89 +/- SE 0.156 for diets containing 0 to 30 g of CaPFA/kg of DM, respectively). Plasma concentrations of growth hormone, insulin-like growth factor-I and leptin did not vary between diets. The number of small (<5 mm) ovarian follicles was negatively related to plasma insulin concentration (r = -0.328) and was stimulated by CaPFA supplementation at all rates tested compared with cows receiving zero CaPFA (small follicles preovulation: 6.7, 11.2, 11.5, 11.3, 11.9 +/- SE 1.48 for diets containing 0 to 30 g of CaPFA/kg of DM, respectively). The number of medium-sized follicles, and diameters of the ovulatory follicles and corpora lutea, were not affected by CaPFA supplementation. It is concluded that dietary total fat concentration should be below 50 g/kg of DM to avoid depressing plasma insulin concentration in cows at the start of the breeding period.
Garnsworthy
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University of Nottingham, School of Biosciences, Sutton Bonington Campus, Loughborough, UK. Phil.Garnsworthy@nottingham.ac.uk
Lock
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Mann
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Sinclair
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Webb
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D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001835:Body Weight / Q000502:physiology; D002417:Cattle / Q000378:metabolism / Q000502:physiology*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D004435:Eating / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D005298:Fertility / Q000502:physiology*; D006728:Hormones / Q000097:blood; D007774:Lactation / Q000502:physiology; D008892:Milk / Q000378:metabolism; D010053:Ovary / Q000502:physiology*; D011897:Random Allocation; D012044:Regression Analysis
D005227:Fatty Acids; D006728:Hormones