[Multiple amplification of 16S rRNA gene and Cytb gene in mitochondrial DNA for species identification].
OBJECTIVE
To establish a fluorescent multiple amplification system of 16S rRNA and Cytb genes located in mitochondrial DNA for species identification.


METHODS
A pair of primers of 16S rRNA gene and Cytb gene of the mitochondrial DNA was designed with the software Primer 5.0 to construct a multiple amplification system. The amplified products from human and five species of animals, including cattle, pig, dog, chicken and grass carp were analyzed by 310 Genetic Analyzer.


RESULTS
The amplified products of these samples showed two peaks. The common one was 358bp and the specific one different in unique species was between 231bp and 256bp.


CONCLUSIONS
The multiplex amplification system can exactly distinguish the species of human from five common animals.
Ye
Yi
Y
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. yeyiconan@sina.com
Wu
Jin
J
Luo
Hai-bo
HB
Wang
Zhuo
Z
Li
Ying-bi
YB
D000818:Animals; D001483:Base Sequence; D002347:Carps; D002417:Cattle; D002645:Chickens; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004285:Dogs; D005260:Female; D053803:Forensic Genetics; D005784:Gene Amplification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013045:Species Specificity; D013552:Swine
D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S; D045303:Cytochromes b