[Effect of the stem cell factor on the morphology and functional state of mouse embryonic stem cells].
The effect of the stem cell factor on the state of membranes and functional activity of mouse embryonic stem cells cultivated in LIF (Leukemia Inhibitory Factor) cytokine-free and LIF-containing media has been studied. It was shown that the stem cell factor induces changes in the viscosity of membrane lipid bilayer and increases the respiration rate, the ATP level, and the proliferation activity of embryonic stem cells. An intricate character of the LIF-dependent modification of biological effects of the stem cell factor was revealed.
Lobanok
E S
ES
Belianovich
L M
LM
Mezhevikina
L M
LM
Volotovskiĭ
I D
ID
Fesenko
E E
EE
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology; D008051:Lipid Bilayers / Q000378:metabolism*; D008560:Membrane Fluidity / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D019089:Stem Cell Factor / Q000494:pharmacology*
D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008051:Lipid Bilayers; D019089:Stem Cell Factor; D000255:Adenosine Triphosphate