Proteomic analysis of mammary tissues from healthy cows and clinical mastitic cows for identification of disease-related proteins.
To investigate the disease-related proteins and understand molecular mechanism of mastitis at the protein level, this project presents the protein changes in the mammary gland between healthy cows and clinical mastitic cows using two-dimensional gel electrophoresis (2-DE), after stained with colloidal Coomassie Bright Blue, six spots of differentially expressed protein were detected by PDQuest software and subjected to ion trap mass spectrometer equipped with a HPLC system, and five proteins were identified. Hemoglobin beta, kappa-casein and tryptophanyl-tRNA-synthetase (TrpRS) in healthy dairy cows, while hemoglobin beta, cytochrome C oxidase and annexin V in clinical mastitic cows were identified, they were involved in binding, transport and catalytic activity. The results may provide valuable information for the investigating of the host mammary immune system response to defense against pathogens at the protein level and potential protein targets for treatment.
Yang
Yong-Xin
YX
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China.
Zhao
Xing-Xu
XX
Zhang
Yong
Y
D000818:Animals; D017304:Annexin A5 / Q000032:analysis; D002364:Caseins / Q000032:analysis; D002417:Cattle / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003576:Electron Transport Complex IV / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D008414:Mastitis, Bovine / Q000097:blood / Q000201:enzymology / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000662:veterinary; D014369:Tryptophan-tRNA Ligase / Q000032:analysis
D017304:Annexin A5; D002364:Caseins; D006454:Hemoglobins; D003576:Electron Transport Complex IV; D014369:Tryptophan-tRNA Ligase