[Fluorescence of age in serum in detecting liver cirrhosis and hepatocellular carcinoma among patients with anti-HCV antibodies].
OBJECTIVE
To evaluate usefulness of total fluorescence of advanced glycation end products (AGE) and haptoglobin (Hp) measurement in human serum as parameters in liver cirrhosis and hepatocellular carcinoma development among patients with anti-HCV antibodies.


METHODS
43 patients (20 women and 23 men) with chronic hepatitis HCV were examined (14 individuals with liver cirrhosis and 9 with primary liver cancer). The control group numbers 20. As reflection of AGE concentration, total fluorescence in serum samples was measured with spectrofluorimetric method and haptoglobin with microplate guaiacol test.


RESULTS
We affirmed that total fluorescence in examined groups was higher than in healthy subjects, but increase was not statistically significant. Fluorescence of AGE in serum from patients with hepatocellular carcinoma was lower in comparison to patients with cirrhosis and anti-HCV carriers. Haptoglobin was significantly decreased in serum of cirrhosis patients as compared to HCV carriers of patients with hepatocellular carcinoma (p < 0.001).


CONCLUSIONS
The measurement of total fluorescence AGE is not differentiating for liver cirrhosis and hepatocellular carcinoma among anty-HCV carriers. We confirmed that haptoglobin, parameter included in fibrotest, is very useful, in detecting liver cirrhosis.
Górka
Joanna
J
Katedra i Zakład Biochemii Farmaceutycznej AM we Wrocławiu. jgorka@op.pl
Zuwała-Jagiełło
Jolanta
J
Pazgan-Simon
Monika
M
Simon
Krzysztof
K
Warwas
Maria
M
D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000821:virology; D016022:Case-Control Studies; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood*; D018937:Hepatitis C Antibodies / Q000097:blood*; D019698:Hepatitis C, Chronic / Q000097:blood* / Q000150:complications; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000821:virology; D008113:Liver Neoplasms / Q000097:blood* / Q000821:virology; D008297:Male; D008875:Middle Aged; D011044:Poland; D013050:Spectrometry, Fluorescence / Q000379:methods
D017127:Glycation End Products, Advanced; D018937:Hepatitis C Antibodies