Directed evolution of streptavidin variants using in vitro compartmentalization.
We have developed and implemented an in vitro compartmentalization (IVC) selection scheme for the identification of streptavidin (SA) variants with altered specificities for the biotin analog desthiobiotin. Wild-type SA and selected variants bind desthiobiotin with similar affinities (approximately 10(-13) M), but the variants have off rates almost 50 times slower and a half-life for dissociation of 24 hr at 25 degrees C. The utility of streptavidin variants with altered specificities and kinetic properties was shown by constructing protein microarrays that could be used to differentially organize and immobilize DNAs bearing these ligands. The methods we have developed should prove to be generally useful for generating a variety of novel SA reagents and for evolving other extremely high-affinity protein:ligand couples.
Levy
Matthew
M
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA. mattlevy@aecom.yu.edu
Ellington
Andrew D
AD
D004247:DNA / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D007700:Kinetics; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding; D019809:Streptavidin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
D004247:DNA; D019809:Streptavidin