title: Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus.
abstract: Lysophosphatidic acid (LPA) modulates prostaglandin (PG) synthesis via LPA receptor 3 (LPAR3) in the murine endometrium. The lack of functional LPAR3 in mice may lead to embryo mortality. In the present study, we examined the role of LPA in the bovine uterus. We confirmed that LPA is locally produced and released from the bovine endometrium. Moreover, there are enzymes involved in LPA synthesis (phospholipase (PL) D(2) and PLA2G1B) in the bovine endometrium during estrous cycle and early pregnancy. Expression of the receptor for LPA (LPAR1) was positively correlated with the expression of PGE(2) synthase (PGES) and negatively correlated with the expression of PGF(2alpha) synthase (aldose reductase with 20 alpha-hydroxysteroid dehydrogenase activity - PGFS) during early pregnancy. In vivo LPA induced P4 and PGE(2) secretion was inhibited by LPAR1 antagonist (Ki16425). The overall results indicate that LPA is locally produced and released from the bovine endometrium. Moreover, LPAR1 gene expression in the endometrium during the estrous cycle and early pregnancy indicates that LPA may play autocrine and/or paracrine roles in the bovine uterus. LPAR1 gene expression is positively correlated with the expression of the enzyme responsible for luteotropic PGE(2) production (PGES) in endometrium. In cow, LPA stimulates P4 and PGE(2) secretion. Thus, LPA in the bovine reproductive tract may indirectly (via endometrium) or directly support corpus luteum action via the increase of P4 synthesis and the increase of PGE(2)/PGF(2)(alpha) ratio. It suggests that LPA may serve as an important factor in the maintenance of early pregnancy in cow.
authors.affiliation: Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Woclawek-Potocka
authors.forename: Junichi
authors.initials: J
authors.lastname: Komiyama
authors.forename: Jean Sebastian
authors.initials: JS
authors.lastname: Saulnier-Blache
authors.forename: Edyta
authors.initials: E
authors.lastname: Brzezicka
authors.forename: Mamadou Moussa
authors.initials: MM
authors.lastname: Bah
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okuda
authors.forename: Dariusz J
authors.initials: DJ
authors.lastname: Skarzynski
mesh_terms: D000818:Animals; D002417:Cattle; D003340:Corpus Luteum Maintenance / Q000187:drug effects*; D015232:Dinoprostone / Q000235:genetics / Q000378:metabolism*; D004717:Endometrium / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D030762:Estrous Cycle / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006911:Hydroxyprostaglandin Dehydrogenases / Q000032:analysis / Q000235:genetics; D019746:Intramolecular Oxidoreductases / Q000032:analysis / Q000235:genetics; D007555:Isoxazoles / Q000494:pharmacology; D008246:Lysophospholipids / Q000032:analysis / Q000097:blood / Q000494:pharmacology*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D011422:Propionates / Q000494:pharmacology; D000071876:Prostaglandin-E Synthases; D012333:RNA, Messenger / Q000032:analysis; D049368:Receptors, Lysophosphatidic Acid / Q000037:antagonists & inhibitors / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: C477898:3-(4-(4-((1-(2-chlorophenyl)ethoxy)carbonyl amino)-3-methyl-5-isoxazolyl) benzylsulfanyl) propanoic acid; D007555:Isoxazoles; D008246:Lysophospholipids; D011422:Propionates; D012333:RNA, Messenger; D049368:Receptors, Lysophosphatidic Acid; D011374:Progesterone; D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone; C032881:lysophosphatidic acid