title: Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
abstract: An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction map focused on various aspects of chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component of the RNA interference (RNAi) machinery (rsh1) and linked the RNAi pathway to several other biological processes. Comparison of the S. pombe E-MAP to an analogous genetic map from the budding yeast revealed that, whereas negative interactions were conserved between genes involved in similar biological processes, positive interactions and overall genetic profiles between pairs of genes coding for physically associated proteins were even more conserved. Hence, conservation occurs at the level of the functional module (protein complex), but the genetic cross talk between modules can differ substantially.
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
authors.forename: Assen
authors.initials: A
authors.lastname: Roguev
authors.forename: Sourav
authors.initials: S
authors.lastname: Bandyopadhyay
authors.forename: Martin
authors.initials: M
authors.lastname: Zofall
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.forename: Tamas
authors.initials: T
authors.lastname: Fischer
authors.forename: Sean R
authors.initials: SR
authors.lastname: Collins
authors.forename: Hongjing
authors.initials: H
authors.lastname: Qu
authors.forename: Michael
authors.initials: M
authors.lastname: Shales
authors.forename: Han-Oh
authors.initials: HO
authors.lastname: Park
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Hayles
authors.forename: Kwang-Lae
authors.initials: KL
authors.lastname: Hoe
authors.forename: Dong-Uk
authors.initials: DU
authors.lastname: Kim
authors.forename: Trey
authors.initials: T
authors.lastname: Ideker
authors.forename: Shiv I
authors.initials: SI
authors.lastname: Grewal
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Weissman
authors.forename: Nevan J
authors.initials: NJ
authors.lastname: Krogan
mesh_terms: D004260:DNA Repair; D004261:DNA Replication; D004843:Epistasis, Genetic; D015966:Gene Expression Regulation, Fungal; D053263:Gene Regulatory Networks; D005800:Genes, Fungal; D006657:Histones / Q000378:metabolism; D009154:Mutation; D034622:RNA Interference; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D006657:Histones; D029702:Schizosaccharomyces pombe Proteins