title: Trafficking of chlamydial antigens to the endoplasmic reticulum of infected epithelial cells.
abstract: Confinement of the obligate intracellular bacterium Chlamydia trachomatis to a membrane-bound vacuole, termed an inclusion, within infected epithelial cells neither prevents secretion of chlamydial antigens into the host cytosol nor protects chlamydiae from innate immune detection. However, the details leading to chlamydial antigen presentation are not clear. By immunoelectron microscopy of infected endometrial epithelial cells and in isolated cell secretory compartments, chlamydial major outer membrane protein (MOMP), lipopolysaccharide (LPS) and the inclusion membrane protein A (IncA) were localized to the endoplasmic reticulum (ER) and co-localized with multiple ER markers, but not with markers of the endosomes, lysosomes, Golgi nor mitochondria. Chlamydial LPS was also co-localized with CD1d in the ER. Since the chlamydial antigens, contained in everted inclusion membrane vesicles, were found within the host cell ER, these data raise additional implications for antigen processing by infected uterine epithelial cells for classical and non-classical T cell antigen presentation.
authors.affiliation: Department of Microbiology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA.
authors.forename: David K
authors.initials: DK
authors.lastname: Giles
authors.forename: Priscilla B
authors.initials: PB
authors.lastname: Wyrick
mesh_terms: D017951:Antigen Presentation; D000942:Antigens, Bacterial / Q000378:metabolism*; D045744:Cell Line, Tumor; D002692:Chlamydia trachomatis / Q000276:immunology* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D004847:Epithelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis; D016253:Microscopy, Immunoelectron
chemical_list: D000942:Antigens, Bacterial; D008070:Lipopolysaccharides