Molecular epidemiology and antimicrobial susceptibilities of 273 exfoliative toxin-encoding-gene-positive Staphylococcus aureus isolates from patients with impetigo in Japan.
The molecular epidemiology and antimicrobial susceptibilities of 273 Staphylococcus aureus isolates positive for the exfoliative toxin-encoding gene obtained from patients with impetigo in Japan in 2006 were studied. The mecA gene was detected in 74 meticillin-resistant S. aureus (MRSA) and 23 meticillin-susceptible S. aureus (MSSA) isolates. All isolates with the staphylococcal cassette chromosome (SCC) mec were classified into type IV (92.8%, 90/97) or V (7.2%, 7/97). The ET-encoding gene etb was found primarily in strains with mecA (87.7%, 71/81), whilst eta (86.6%, 161/186) was detected mainly in strains without mecA. The chromosomal enterotoxin-encoding gene cluster egc was found in 83.0% of strains with eta, whilst no enterotoxin-encoding gene was detected in strains with only etb. PFGE showed that each strain carrying eta, etb and etd could be classified into distinct groups. The susceptibility profiles of MRSA to antimicrobial agents excluding beta-lactams were similar to those of MSSA. Gentamicin- and clarithromycin-resistant strains were frequently found for both MRSA and MSSA. The aminoglycoside-resistance gene aacA-aphD was detected in 97.3% of MRSA and 85.4% of MSSA. Additionally, the macrolide-resistance gene ermA or ermC was detected in 67.6% of MRSA and 71.4% of MSSA. Therefore, these results suggest that SCCmec types IV or V have spread, particularly in MSSA carrying etb in the community.
Nakaminami
Hidemasa
H
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Noguchi
Norihisa
N
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Ikeda
Masami
M
Takamatsu Dermatological Research Group, 4-1-3 Banchou, Takamatsu, Kagawa 760-0017, Japan.|Department of Dermatology, Takamatsu Red Cross Hospital, 4-1-3 Banchou, Takamatsu, Kagawa 760-0017, Japan.
Hasui
Mikiko
M
Takamatsu Dermatological Research Group, 4-1-3 Banchou, Takamatsu, Kagawa 760-0017, Japan.
Sato
Minoru
M
Takamatsu Dermatological Research Group, 4-1-3 Banchou, Takamatsu, Kagawa 760-0017, Japan.
Yamamoto
Shinji
S
Takamatsu Dermatological Research Group, 4-1-3 Banchou, Takamatsu, Kagawa 760-0017, Japan.
Yoshida
Tomoko
T
Takamatsu Dermatological Research Group, 4-1-3 Banchou, Takamatsu, Kagawa 760-0017, Japan.
Asano
Takashi
T
Takamatsu Dermatological Research Group, 4-1-3 Banchou, Takamatsu, Kagawa 760-0017, Japan.
Senoue
Mitsura
M
Takamatsu Dermatological Research Group, 4-1-3 Banchou, Takamatsu, Kagawa 760-0017, Japan.
Sasatsu
Masanori
M
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002648:Child; D024901:Drug Resistance, Multiple, Bacterial / Q000502:physiology; D005083:Exfoliatins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007169:Impetigo / Q000453:epidemiology* / Q000382:microbiology*; D007564:Japan / Q000453:epidemiology; D017720:Molecular Epidemiology; D010802:Phylogeny; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
D000900:Anti-Bacterial Agents; D005083:Exfoliatins