Effects of dietary meals containing different levels of phytoestrogens on reproductive function in Bilgoraj ganders.
The aim of this study was to investigate how long-term feeding of ganders with diets containing different levels of phytoestrogens affects the morphometry of testes, plasma steroid levels, sperm parameters and reproductive performance. Male Bilgoraj geese were fed diets containing grass meal (with low phytoestrogen levels) and those containing alfalfa meal and soybean meal (with higher levels of phytoestrogens). Testes were obtained from ganders at the peak of mating and the ongoing laying period as well as at the beginning of photorefractoriness. The morphometric parameters were measured in frozen testicular sections. Blood was collected monthly from December to June, and plasma testosterone, androstenedione and corticosterone concentrations were determined. During the mating season, semen was collected and its quantity and quality were investigated. In the flocks (ganders with geese) reproductive output was noted. Seasonal changes were observed in the morphology of testes and in plasma testosterone, androstenedione and corticosterone concentrations in ganders. The feeding of ganders with dietary meals containing higher levels of phytoestrogens did not affect the plasma steroid contents or the majority of the morphometric parameters of testes, except for the height of the seminiferous epithelium, which was lowered. Moreover, diets with higher levels of phytoestrogens decreased the volume of ejaculates and the percentage of normal spermatozoa. However, these changes were not reflected in the fertilisation rates.
Opalka
Marek
M
Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland. mareko@uwm.edu.pl
Kugla-Owczarska
Justyna
J
Kaminska
Barbara
B
Puchajda-Skowronska
Helena
H
Hryniewicka
Wioletta
W
Dusza
Luiza
L
D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology*; D000818:Animals; D005777:Geese / Q000502:physiology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D048789:Phytoestrogens / Q000008:administration & dosage* / Q000494:pharmacology; D011897:Random Allocation; D012098:Reproduction / Q000502:physiology*; D012621:Seasons; D012661:Semen / Q000166:cytology / Q000502:physiology*; D013737:Testis / Q000033:anatomy & histology / Q000502:physiology; D013739:Testosterone / Q000032:analysis / Q000378:metabolism*
D048789:Phytoestrogens; D013739:Testosterone