Efficient induction of oral tolerance by fusing cholera toxin B subunit with allergen-specific T-cell epitopes accumulated in rice seed.
Cholera toxin B (CTB) subunit is an efficient mucosal carrier molecule for induction of oral tolerance to antigens and allergens. Here, T-cell epitopes of Cry j 1 and Cry j 2, major allergens in Japanese cedar pollen, were expressed in rice seed as a fusion protein with either CTB or rice glutelin as a control. Feeding mice with rice seed containing CTB-fused T-cell epitopes suppressed allergen-specific IgE responses and pollen-induced clinical symptoms at 50-fold lower doses of T-cell epitopes than required when using control seed. Our findings present a novel potential strategy for immunotherapy of type-I allergy.
Takagi
Hidenori
H
Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, Japan. hikatagi@affrc.go.jp
Hiroi
Takachika
T
Yang
Lijun
L
Takamura
Kaoru
K
Ishimitsu
Ryotaro
R
Kawauchi
Hideyuki
H
Takaiwa
Fumio
F
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000485:Allergens / Q000235:genetics* / Q000494:pharmacology*; D000818:Animals; D002772:Cholera Toxin / Q000737:chemistry* / Q000494:pharmacology*; D032485:Cryptomeria / Q000235:genetics* / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D018984:Epitopes, T-Lymphocyte / Q000187:drug effects / Q000276:immunology*; D006636:Histamine Release / Q000187:drug effects; D007108:Immune Tolerance / Q000187:drug effects*; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012275:Oryza / Q000235:genetics* / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D011058:Pollen / Q000276:immunology; D012639:Seeds / Q000276:immunology; D014170:Transformation, Genetic
D000276:Adjuvants, Immunologic; D000485:Allergens; D018984:Epitopes, T-Lymphocyte; D007073:Immunoglobulin E; D002772:Cholera Toxin