3,4-Dihydroxyphenylalanine gel diffusion assay for polyphenol oxidase quantification.
We have developed a simple, inexpensive plate assay to detect and quantify polyphenol oxidase (PPO) activity from different origins. The logarithm of enzyme activity is linearly correlated with the diameter of the dark, l-3,4-dihydroxyphenylalanine (l-DOPA) oxidized circles produced in the gel, thereby allowing quantification of PPO. Moreover, precision and high reproducibility of the assay were confirmed by statistical analysis.
Zocca
Federico
F
Dipartimento di Biotecnologie Agrarie, Università di Padova, Viale dell' Universita 16, Agripolis, 35020 Legnaro, Padova, Italy.
Lomolino
Giovanna
G
Lante
Anna
A
D004156:Catechol Oxidase / Q000378:metabolism*; D004058:Diffusion; D004295:Dihydroxyphenylalanine / Q000378:metabolism*; D005782:Gels; D016014:Linear Models; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005782:Gels; D004295:Dihydroxyphenylalanine; D004156:Catechol Oxidase