Plant regeneration from cell suspension culture of potato (Solanum tuberosum L.).
In the present study, for callus production leaf and stem segments of potato cultivar White Desiree were cultured on MS medium supplemented with 2,4-D, NAA and Kinetin (callus production medium). Calli then were transferred in the same liquid medium for cell suspension production. In the next step cell suspensions were transferred back to the callus production medium. Finally, calli derived from cell suspension were cultured on 6 different shoot initiation media (S1-S6). However, on S6 medium with combination of GA3 and BAP more than 80% of the calli produced shoot buds and shoots. Fully grown shoots then were rooted and produced whole plants. Chromosome and morphological analysis showed no somaclonal variation among regenerated plants.
Torabi
Firozeh
F
Department of Biology, Faculty of Science, University of Tarbiat Moalem, Tehran, Iran.
Majad
Ahmad
A
Ehsanpour
Ali Akbar
AA
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D001901:Botany / Q000379:methods*; D018929:Cell Culture Techniques; D002875:Chromosomes / Q000648:ultrastructure; D003470:Culture Media / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007701:Kinetin / Q000494:pharmacology; D009154:Mutation; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D018521:Plant Physiological Phenomena; D010944:Plants / Q000187:drug effects; D012038:Regeneration / Q000187:drug effects*; D011198:Solanum tuberosum / Q000166:cytology / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D009280:Naphthaleneacetic Acids; D015084:2,4-Dichlorophenoxyacetic Acid; D007701:Kinetin