Construction of SSR-based chromosome map in bunching onion (Allium fistulosum).
We have constructed a linkage map of bunching onion (Allium fistulosum L., 2n = 16) using an F(2) population of 225 plants. The map consists of 17 linkage groups with 212 bunching onion SSR markers and 42 bulb onion (A. cepa L.) SSR, InDel, CAPS or dCAPS markers, covering 2,069 cM. This is the first report of a linkage map mainly based on SSR markers in the genus Allium. With the 103 anchor markers [81 bunching onion SSRs, 11 bulb onion SSRs and 11 bulb onion non-SSRs (1 InDel, 9 CAPSs and 1 dCAPS)] whose chromosome assignments were identified in A. cepa and/or A. fistulosum, via the use of several kinds of Allium alien addition lines, 16 of the 17 linkage groups were connected to the 8 basic chromosomes of A. cepa.
Tsukazaki
Hikaru
H
National Institute of Vegetable and Tea Science (NIVTS), NARO, 360 Ano-Kusawa, Tsu, Mie, 514-2392, Japan.
Yamashita
Ken-Ichiro
K
Yaguchi
Shigenori
S
Masuzaki
Shinichi
S
Fukuoka
Hiroyuki
H
Yonemaru
Junichi
J
Kanamori
Hiroyuki
H
Kono
Izumi
I
Hang
Tran Thi Minh
TT
Shigyo
Masayoshi
M
Kojima
Akio
A
Wako
Tadayuki
T
D000490:Allium / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D054643:INDEL Mutation; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018744:DNA, Plant