title: [Study on pharmacokinetics of glycyrrhetic acid in rats by RP-HPLC].
abstract: OBJECTIVE
  To develop a HPLC for the determination of plasma concentration of glycyrrhetic acid (GA) and study the pharmacokinetics of GA in rats.
  
  
  METHODS
  To sample blood from rat which were injected GA by 1.0 mg x kg(-1) at 0.08, 0.17, 0.25, 0.33, 0.5, 0.75, 1, 2, 3, 4, 6 h and use HPLC to determine the concentration of GA in it, the pharmaeokinetie parameters were accessed by DAS 1.0.
  
  
  RESULTS
  The calibration curve was linear (r = 0.999 7) within the range of 50-2 000 ng x mL(-1) for GA in plasma. The average recovery was (105.2 +/- 2.23)%, (102.5 +/- 2.95)%, (98.4 +/- 2.32)%. The within-day and between-day derivation (RSD) were less than 4%. GA was fitted to a two compartment open pharmaeokinetie model in rats. The mainly pharmaeokinetie parameters were: t1/2alpha = (0.153 +/- 0.023) h, t1/2beta = (2.365 +/- 0.866) h, C(max) = (2.074 +/- 0.100) mg x L(-1), CL = (0.715 +/- 0.082) L x h(-1) x kg(-1), V(d) = (2.427 +/- 0.872) L x kg(-1), AUC(0-6 h) = (1.302 +/- 0.151) mg x h(-1) x L(-1).
  
  
  CONCLUSIONS
  The method can be used to determine the concentration and to investigate the pharmacokinetics of GA in rat. GA was disposed extensively and rapidly in rats.
authors.affiliation: Beijing University of TCM, Beijing 100102, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Shou-Ying
authors.initials: SY
authors.lastname: Du
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006034:Glycyrrhetinic Acid / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006034:Glycyrrhetinic Acid