Effect of intraoperative mitomycin-C on healthy corneal endothelium after laser-assisted subepithelial keratectomy.
OBJECTIVE
To evaluate the effect of mitomycin-C (MMC) on corneal endothelial cells after laser-assisted subepithelial keratectomy (LASEK).


METHODS
Department of Ophthalmology, Changzheng Hospital, Shanghai, China.


METHODS
One hundred seventy-four eyes of 89 patients who did not previously wear contact lenses were treated with LASEK with intraoperative use of topical MMC 0.02% (15 seconds). Noncontact corneal specular microscopy was performed in all eyes preoperatively and 1, 3, and 6 months after surgery. Preoperative pachymetry and ablation depth were measured in all eyes. Repeated-measures analysis of variance was used to compare the changes in the endothelial central cell density (CCD), coefficient of variation in cell size (CV), and percentage of hexagram cells (HEX) over time. Linear regression analysis was conducted to analyze the correlation between the change in the 3 corneal endothelium indices over time and the ablation depth and residual stroma bed (RSB) thickness.


RESULTS
Preoperatively, the mean CCD was 2755 cells/mm(2)+/-373 (SD), the mean CV was 31.45+/-8.26, and the mean HEX was 66.03%+/-25.83%. After LASEK, there were no statistically significant changes in CCD, CV, or HEX (P>.05). Multiple linear regression did not identify ablation depth or RSB thickness as being predictive of a change in CCD, CV, or HEX (P>.05).


CONCLUSIONS
The use of intraoperative topical MMC 0.02% for 15 seconds after LASEK did not affect the corneal endothelium.
Zhao
Li-Quan
LQ
Department of Ophthalmology, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai, China.
Wei
Rui-Li
RL
Ma
Xiao-Ye
XY
Zhu
Huang
H
D000293:Adolescent; D000328:Adult; D000477:Alkylating Agents / Q000494:pharmacology*; D001251:Astigmatism / Q000601:surgery; D002452:Cell Count; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007430:Intraoperative Care; D049008:Keratectomy, Subepithelial, Laser-Assisted; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D009216:Myopia / Q000601:surgery*; D011446:Prospective Studies; D055815:Young Adult
D000477:Alkylating Agents; D016685:Mitomycin