Determination of two metabolites of calycosin-7-O-beta-D-glucopyranoside in rat urine by HPLC.
A simple and specific analytical method for the simultaneous determination of the two metabolites of calycosin-7-O-beta-D-glucopyranoside, calycosin-7-O-beta-D-glucuronic acid methyl ester (M-1) and calycosin (M-2), in rat urine was developed using high-performance liquid chromatography. Quercetin was employed as an internal standard. The correlation coefficients of the calibration curves were higher than 0.999; both intra- and inter-day precisions of two metabolites were determined and their RSD did not exceed 10%. The accuracy and linear range were investigated in detail. The cumulative urinary excretions of two metabolites were measured.
Yang
Yan-Ling
YL
Department of Physiology, Medical College of Yan'an University, Yan'an, People's Republic of China. yyl8889@sina.com
Xia
Guang-Xin
GX
Ye
Guan
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D007529:Isoflavones / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
D005960:Glucosides; D007529:Isoflavones; C488311:calycosin-7-O-glucopyranoside