title: Galectin-3 regulates integrin alpha2beta1-mediated adhesion to collagen-I and -IV.
abstract: Galectins are a taxonomically widespread family of galactose-binding proteins of which galectin-3 is known to modulate cell adhesion. Using single cell force spectroscopy, the contribution of galectin-3 to the adhesion of Madin-Darby canine kidney (MDCK) cells to different extracellular matrix proteins was investigated. When adhering to collagen-I or -IV, some cells rapidly entered an enhanced adhesion state, marked by a significant increase in the force required for cell detachment. Galectin-3-depleted cells had an increased probability of entering the enhanced adhesion state. Adhesion enhancement was specific to integrin alpha(2)beta(1), as it was not observed when cells adhered to extracellular matrix substrates by other integrins. The adhesion phenotype of galectin-3-depleted cells was mimicked in a galactoside-deficient MDCK cell line and could be complemented by the addition of recombinant galectin-3. We propose that galectin-3 influences integrin alpha(2)beta(1)-mediated adhesion complex formation by altering receptor clustering.
authors.affiliation: Biotechnology Center, University of Technology Dresden, 01307 Dresden Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Friedrichs
authors.forename: Aki
authors.initials: A
authors.lastname: Manninen
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Muller
authors.forename: Jonne
authors.initials: J
authors.lastname: Helenius
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D024042:Collagen Type I / Q000378:metabolism*; D024141:Collagen Type IV / Q000378:metabolism*; D004285:Dogs; D005109:Extracellular Matrix / Q000378:metabolism; D037502:Galectin 3 / Q000378:metabolism / Q000502:physiology*; D005786:Gene Expression Regulation; D038982:Integrin alpha2beta1 / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D011994:Recombinant Proteins / Q000737:chemistry; D013314:Stress, Mechanical
chemical_list: D024042:Collagen Type I; D024141:Collagen Type IV; D037502:Galectin 3; D038982:Integrin alpha2beta1; D011994:Recombinant Proteins