title: Construction of SSR-based chromosome map in bunching onion (Allium fistulosum).
abstract: We have constructed a linkage map of bunching onion (Allium fistulosum L., 2n = 16) using an F(2) population of 225 plants. The map consists of 17 linkage groups with 212 bunching onion SSR markers and 42 bulb onion (A. cepa L.) SSR, InDel, CAPS or dCAPS markers, covering 2,069 cM. This is the first report of a linkage map mainly based on SSR markers in the genus Allium. With the 103 anchor markers [81 bunching onion SSRs, 11 bulb onion SSRs and 11 bulb onion non-SSRs (1 InDel, 9 CAPSs and 1 dCAPS)] whose chromosome assignments were identified in A. cepa and/or A. fistulosum, via the use of several kinds of Allium alien addition lines, 16 of the 17 linkage groups were connected to the 8 basic chromosomes of A. cepa.
authors.affiliation: National Institute of Vegetable and Tea Science (NIVTS), NARO, 360 Ano-Kusawa, Tsu, Mie, 514-2392, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Tsukazaki
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Yamashita
authors.forename: Shigenori
authors.initials: S
authors.lastname: Yaguchi
authors.forename: Shinichi
authors.initials: S
authors.lastname: Masuzaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fukuoka
authors.forename: Junichi
authors.initials: J
authors.lastname: Yonemaru
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kanamori
authors.forename: Izumi
authors.initials: I
authors.lastname: Kono
authors.forename: Tran Thi Minh
authors.initials: TT
authors.lastname: Hang
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Shigyo
authors.forename: Akio
authors.initials: A
authors.lastname: Kojima
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Wako
mesh_terms: D000490:Allium / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D054643:INDEL Mutation; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant