Protective effects of TREK-1 against oxidative injury induced by SNP and H2O2.
OBJECTIVE
TREK-1 (TWIK-related K+ channel-1) is a 2-pore-domain K+ channel subtype. The present study investigated the role of TREK-1 in cell death induced by oxidative stress.


METHODS
The cell viability of wild-type Chinese hamster ovary (CHO) and TREK-1-transfected CHO cells (TREK-1/CHO cells) was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in the presence of sodium nitroprusside (SNP) or hydrogen peroxide (H2O2). Apoptosis of wild-type CHO and TREK-1/CHO cells was detected using Hoechst33342 staining.


RESULTS
Both SNP and H2O2 caused dose- and time-dependent growth inhibition of wild-type CHO and TREK-1/ CHO cells. Following a 12 h exposure to SNP, the 50% inhibition (IC(50)) values for wild-type CHO and TREK-1/CHO cells were calculated as 0.69 mmol/L and 1.14 mmol/L, respectively. The IC(50) values were 0.07 mmol/L and 0.09 mmol/L in H2O2-treated wild-type CHO and TREK-1/CHO cells, respectively, following 12 h exposure to H2O2. Moreover, SNP/H2O2 induced less apoptosis in TREK-1/ CHO cells than that in wild-type CHO cells (P<0.05).


CONCLUSIONS
The results demonstrated that TREK-1 played a protective role against oxidative injury.
Sun
Li-na
LN
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Li
Liao-liao
LL
Li
Zheng-bin
ZB
Wang
Ling
L
Wang
Xiao-liang
XL
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004594:Electrophysiology; D006861:Hydrogen Peroxide / Q000633:toxicity*; D009599:Nitroprusside / Q000633:toxicity*; D016877:Oxidants / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D024683:Potassium Channels, Tandem Pore Domain / Q000502:physiology*
D016877:Oxidants; D024683:Potassium Channels, Tandem Pore Domain; C103742:potassium channel protein TREK-1; D009599:Nitroprusside; D006861:Hydrogen Peroxide