[Effect of mesenteric lymphatic duct ligation on the system inflammation during the intestinal ischemia-reperfusion].
OBJECTIVE
To estimate the effect of the lymph duct ligation on systemic inflammatory factors and endotoxins during intestinal ischemia-reperfusion (I/R).


METHODS
Male SD rats underwent occlusion of superior mesenteric artery for 60 min followed by reperfusion for 120 min plus lymph duct ligation or not. Forty rats were randomly divided into 4 groups: group A (blank); group B (sham); group C (intestinal I/R); group D (intestinal I/R plus lymph duct ligation). Mesenteric lymph nodes were harvested for standard bacteriologic cultures. The endotoxin, D-lactate, diamine oxidase (DAO), and cytokines in serum were detected.


RESULTS
The rates of bacterial translocation to mesenteric lymph nodes were 40% in group C and 20% in group D. No positive lymph node cultures were encountered in any of group A and B. The serum cytokines (except for sICAM-1) , D-lactate, DAO and endotoxin levels were lower in group D than those in group C (P<0.05), but both were higher than those in group A and B (P<0.05).


CONCLUSIONS
During intestinal I/R injury, blockage the lymph flow from gut into bloodstream decreases the levels of cytokines, and significantly attenuates the increase in intestinal permeability.
He
Gui-zhen
GZ
Department of Enteral and Parenteral Nutrition, PUMC Hospital, CAMS and PUMC, Beijing, China. hgzpumc@163.com
Dong
Liang-guang
LG
Cui
Xiao-yu
XY
Chen
Xue-feng
XF
Shu
Hong
H
D000818:Animals; D004195:Disease Models, Animal; D007249:Inflammation; D007410:Intestinal Diseases / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D007422:Intestines / Q000098:blood supply / Q000473:pathology*; D008026:Ligation; D008198:Lymph Nodes / Q000473:pathology*; D008208:Lymphatic System / Q000601:surgery; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*