A novel and rapid method for determining integration frequency catalyzed by integron integrase intI1.
We developed a faster and more convenient method to determine integration frequency mediated by integron integrase intI1. This method based on real-time fluorescent quantitative PCR. By using this method, we revealed that the integration frequency of aadA2 gene cassette was 1.87x10(-4) when integrase intI1 was present, and the background frequency was less than 5.23x10(-8) without integrase intI1.
Yang
Zehua
Z
Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China.
Jiang
Xiaofei
X
Wei
Quhao
Q
Chen
Nan
N
Lu
Yuan
Y
D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005453:Fluorescence; D018628:Gene Dosage; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D032023:Integrons; D016133:Polymerase Chain Reaction / Q000379:methods*; D011995:Recombination, Genetic
D019426:Integrases; C115006:integron integrase IntI1