Developmental regulation of neuromodulator function in the stomatogastric ganglion of the lobster, Homarus americanus.
Neuromodulatory substances have profound effects on the two motor patterns generated by the adult crustacean stomatogastric ganglion (STG), the gastric mill rhythm and the pyloric rhythm. Developmentally regulated changes in the modulatory functions of neuromodulators could therefore play an important role in the maturation of the output from the developing STG. We compared the effects of neuromodulators on isolated embryonic and adult STG of the lobster, Homarus americanus. Bath application of Val(1)-SIFamide, a peptide whose expression is different in embryos and adults, activated different neuron classes in embryos and adults. Cancer borealis tachykinin-related peptide 1a, a peptide that does not appear in the terminals of modulatory neurons in the STG until after embryonic development, also produced different motor patterns in embryos and adults. In contrast, red pigment concentrating hormone, a peptide with a similar distribution in the STNS across development, produced similar motor patterns in embryonic and adult STG. Proctolin, serotonin, and allatostatin were also physiologically active on the isolated embryonic STG. Together, these results demonstrate that receptors to many neuromodulators are present and functional on STG neurons before the motor patterns of the stomatogastric nervous system are mature. Moreover, neuromodulator responses change during development, perhaps contributing to the maturation of the output from the stomatogastric nervous system.
Rehm
Kristina J
KJ
Volen Center, Brandeis University, Waltham, Massachusetts 02454-9110, USA. Kristina_Rehm@DFCI.Harvard.edu
Deeg
Katherine E
KE
Marder
Eve
E
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal; D004625:Embryo, Nonmammalian; D017952:Ganglia, Invertebrate / Q000166:cytology* / Q000187:drug effects / Q000196:embryology*; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology; D018613:Microscopy, Confocal / Q000379:methods; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009415:Nerve Net / Q000254:growth & development / Q000502:physiology; D018377:Neurotransmitter Agents / Q000494:pharmacology / Q000502:physiology*; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology; D033521:Palinuridae; D011708:Pylorus / Q000294:innervation / Q000502:physiology; D013057:Spectrum Analysis; D013270:Stomach / Q000196:embryology / Q000294:innervation / Q000502:physiology
D037102:Lectins; C102819:NEB-LFamide; D018377:Neurotransmitter Agents; D009842:Oligopeptides; C425303:Tachypleus plasma lectin-1