Redmond Red as a redox probe for the DNA-mediated detection of abasic sites.
Redmond Red, a fluoropore containing a redox-active phenoxazine core, has been explored as a new electrochemical probe for the detection of abasic sites in double-stranded DNA. The electrochemical behavior of Redmond Red-modified DNA at gold surfaces exhibits stable, quasi-reversible voltammetry with a midpoint potential centered around -50 mV versus NHE. Importantly, with Redmond Red positioned opposite an abasic site within the DNA duplex, the electrochemical response is significantly enhanced compared to Redmond Red positioned across from a base. Redmond Red, reporting only if well-stacked in the duplex, represents a sensitive probe to detect abasic sites electrochemically in a DNA-mediated reaction.
Buzzeo
Marisa C
MC
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Barton
Jacqueline K
JK
D020029:Base Pairing; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage; D004563:Electrochemistry; D004566:Electrodes; D010078:Oxazines / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction
D010078:Oxazines; D004247:DNA; C039203:phenoxazine