title: Electrocatalytic activity of horseradish peroxidase/chitosan/carbon microsphere microbiocomposites to hydrogen peroxide.
abstract: Colloidal carbon microspheres (CMS) are dispersed in chitosan (CHIT) solution to form an organic-inorganic hybrid with excellent micro-environment for the immobilization of biomolecules. A novel amperometric biosensor for the determination of hydrogen peroxide (H(2)O(2)) has been constructed by entrapping horseradish peroxidase (HRP) in as-synthesized CMS/CHIT hybrid. The modification of glassy carbon electrode is made by a simple solution-evaporation method. The electrochemical properties of the biosensor are characterized in electrochemical methods. The proposed biosensor shows high sensitive determination and fast response to H(2)O(2) at -0.15 V. The constructed HRP/CHIT/CMS/GC electrode also exhibits a fine linear correlation with H(2)O(2) concentration. The calculated value of the apparent Michaelis-Menten constant, 2.33 mM, suggests that the HRP in CMS/CHIT hybrid keeps its native bioactivity and has high affinity for H(2)O(2).
authors.affiliation: Key Laboratory for Micro-Nano Optoelectronic Devices of Ministry of Education, Hunan University, Changsha 410082, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Chen
authors.forename: Chengchao
authors.initials: C
authors.lastname: Li
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Du
authors.forename: Shoujiang
authors.initials: S
authors.lastname: Xu
authors.forename: Limiao
authors.initials: L
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Taihong
authors.initials: T
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D002384:Catalysis; D048271:Chitosan / Q000737:chemistry*; D003102:Colloids; D004563:Electrochemistry; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003102:Colloids; D002244:Carbon; D048271:Chitosan; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase