Arsenic induced toxicity on testicular tissue of mice.
Effect of arsenic was studied on the testicular tissue of Swiss albino mice. Sodium-meta-arsenite (NaAsO2) was administered to adult mice (25 +/- 30 g) at a dose level of 30 mg/L and 40 mg/L through drinking water for 30, 45 and 60 days. After the treatment, the testicular organ was removed, weighed and processed for histopathological observation. No change in the body weight was recorded in treated groups after arsenic exposure but significant decrease in the relative testicular weight was observed in comparison with the control. The result showed that arsenic-treated mice exhibited dose dependent gradual reductions in seminiferous tubular diameter and various gametogenic cell population i.e. resting spermatocyte, pachytene spermatocyte and step-7-spermatid except spermatogonia. Leydig cell atrophy was significantly increased in dose dependent manner indicating a definite effect of arsenic on the spermatogenesis in mice. These observations were supported by gradual reduction in Leydig cell population in the above treated groups. In conclusion, the above results confirm the toxic effect of arsenic in testis of mice.
Sanghamitra
Sarkar
S
Industrial Toxicology Division, Regional Occupational Health Centre (E), Indian Council of Medical Research, Block DP, Sector V, Salt Lake City, Kolkata--700 091.
Hazra
J
J
Upadhyay
S N
SN
Singh
R K
RK
Amal
Roy Chowdhury
RC
D000818:Animals; D018053:Arsenites / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D007985:Leydig Cells / Q000187:drug effects; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D017670:Sodium Compounds / Q000633:toxicity*; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects; D013093:Spermatogonia / Q000187:drug effects; D013733:Testicular Diseases / Q000139:chemically induced* / Q000473:pathology*; D013737:Testis / Q000473:pathology; D016707:Tissue Fixation
D018053:Arsenites; D017670:Sodium Compounds; C017947:sodium arsenite