title: Accelerated wound healing by smad3 antisense oligonucleotides-impregnated chitosan/alginate polyelectrolyte complex.
abstract: Smad3 mediates the intracellular signaling of TGF-beta1 superfamily and plays a critical role in the cellular proliferation, differentiation and elaboration of matrix pivotal to cutaneous wound healing. Smad3 antisense oligonucleotides (ASOs) impregnated polyelectrolyte complex (PEC) containing chitosan and sodium alginate was prepared for accelerated wound healing. Physicochemical properties of PEC were characterized by zeta potential, scanning electron microscopy and bioadhesive test. Full-thickness, excisional wounds were made on the dorsum of C57BL6 mice. Then, smad3 ASOs-PEC, PEC alone, smad3 ASOs and gauze dressing were applied to determine concentration of TGF-beta1 and collagen in tissues and observe the wound contraction and histology of tissues. Zeta potentials and bioadhesive strengths of ASOs-PEC were increased as the chitosan ratio in PEC. In smad3 ASOs-PEC, the healing process suggested by wound closure and histological observation was faster than other groups because collagen contents increased and level of TGF-beta1 decreased. These results demonstrate that the smad3 ASOs-PEC composed of chitosan and sodium alginate could be applied for accelerated wound healing.
authors.affiliation: Laboratory of Excellency for Drug and Gene Delivery, College of Pharmacy, Seoul National University, 599 Kwanangno, Kwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Hyo-Jeong
authors.initials: HJ
authors.lastname: Hong
authors.forename: Su-Eon
authors.initials: SE
authors.lastname: Jin
authors.forename: Jeong-Sook
authors.initials: JS
authors.lastname: Park
authors.forename: Woong Shick
authors.initials: WS
authors.lastname: Ahn
authors.forename: Chong-Kook
authors.initials: CK
authors.lastname: Kim
mesh_terms: D000268:Adhesiveness / Q000187:drug effects; D000464:Alginates / Q000378:metabolism*; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D004573:Electrolytes / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D016376:Oligonucleotides, Antisense / Q000494:pharmacology* / Q000648:ultrastructure; D051900:Smad3 Protein / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000464:Alginates; D004573:Electrolytes; D006603:Hexuronic Acids; D016376:Oligonucleotides, Antisense; D051900:Smad3 Protein; D053773:Transforming Growth Factor beta1; D020723:Glucuronic Acid; D003094:Collagen; D048271:Chitosan