title: Expression analysis of the exo-beta-1,3-glucanase from the mycoparasitic fungus Trichoderma asperellum.
abstract: The regulation of the gene encoding the extracellular exo-beta-1,3-glucanase (tag83) produced by the mycoparasite Trichoderma asperellum was studied. Enzyme activity was detected in all carbon sources, but the highest levels were found when starch and purified cell walls from Rhizoctonia solani were used. These results are supported by the appearance of one strong band with enzyme activity in non-denaturing PAGE. Experiments using RT-PCR showed that exo-beta-1,3-glucanase induction in T. asperellum occurred at the transcriptional level. We used RT-PCR and real-time PCR analysis to examine the expression of tag83 gene during in vivo assay of T. asperellum against R. solani. We showed that the expression of tag83 is significantly increased by the presence of R. solani.
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, 70910-900 DF, Brazil.
authors.forename: César Marcos
authors.initials: CM
authors.lastname: Marcello
authors.forename: Andrei Stecca
authors.initials: AS
authors.lastname: Steindorff
authors.forename: Silvana Petrofeza
authors.initials: SP
authors.lastname: da Silva
authors.forename: Roberto do Nascimento
authors.initials: Rdo N
authors.lastname: Silva
authors.forename: Luiz Artur
authors.initials: LA
authors.lastname: Mendes Bataus
authors.forename: Cirano José
authors.initials: CJ
authors.lastname: Ulhoa
mesh_terms: D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics* / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D043326:Glucan 1,3-beta-Glucosidase