title: Alpha-tocopherol protects against oxidative damage to lipids of the rod outer segments of the equine retina.
abstract: Oxidative stress is a possible risk factor for eye diseases. Lipid peroxidation is one of the major events induced by oxidative stress and is particularly active in polyunsaturated fatty acid (PUFA)-rich biomembranes. This work evaluated endogenous lipid antioxidants, in vitro non-enzymatic lipid peroxidation of rod outer segment membranes (ROS), the fatty acid composition during oxidative damage of total lipids from equine retina and ROS, and the protective action of alpha-tocopherol (alpha-Toc). The major lipid soluble antioxidant was alpha-Toc followed by retinoids and carotenoids. The retina contained a high percentage of PUFAs, mainly docosahexaenoic acid (22:6n-3) and arachidonic acid (20:4n-6). Lipid peroxidation of the equine ROS, induced by Fe(2+)-ascorbate, was monitored using chemiluminescence (CL) with or without pre-treatment with alpha-Toc. With alpha-Toc pre-treatment, CL values were significantly decreased. The most abundant fatty acid was 22:6n-3. After 3h incubation, 95% of total PUFAs were destroyed by peroxidation, whereas in alpha-Toc pre-treated ROS the percentage was significantly decreased. The results show that the retina has an endogenous lipid soluble antioxidant system. ROS were highly sensitive to oxidative damage, since their fatty acid composition was markedly modified during the lipid peroxidation process. The protective role of alpha-Toc as an antioxidant was evident and it could be used in the treatment of equine ocular diseases in which free radicals are involved.
authors.affiliation: Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118, 1900 La Plata, Buenos Aires, Argentina. aterrasa@fcv.unlp.edu.ar
authors.forename: Ana M
authors.initials: AM
authors.lastname: Terrasa
authors.forename: Margarita H
authors.initials: MH
authors.lastname: Guajardo
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Marra
authors.forename: Gustavo
authors.initials: G
authors.lastname: Zapata
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D016166:Free Radical Scavengers; D006736:Horses / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008163:Luminescent Measurements; D018384:Oxidative Stress / Q000187:drug effects*; D012374:Rod Cell Outer Segment / Q000187:drug effects* / Q000378:metabolism; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005231:Fatty Acids, Unsaturated; D016166:Free Radical Scavengers; D024502:alpha-Tocopherol