[1, 3-propanediol production under salt stress].
Through studying the process of glycerol fermentation to 1, 3-propanediol(1, 3-PD) by Klebsiella pneumoniae, it was found that the cell growth and product (or by-product) production were under salt stress. Cell growth and product formation kept high rate at low salt concentration. High salt concentration led to low growth of cells, final concentration of 1, 3-PD and conversion from glycerol to 1, 3-PD, and, 1, 3-propanediol oxidoreductase activity decreased. When the salt concentration in 5 m3 bioreactor was controlled under appropriate manner, the concentration of 1, 3-PD production was markedly enhanced. The final 1, 3-PD concentration ,the conversion of glycerol to 1, 3-PD and productivity were 64 g/L, 61% and 2.1 g/(L x h).
Xu
Jiajie
J
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Liu
Pengbo
P
Tao
Chunping
C
Cheng
Cheng
C
Fu
Shuilin
S
Gong
Heng
H
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D011409:Propylene Glycols / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological
D003470:Culture Media; D011409:Propylene Glycols; D012965:Sodium Chloride; C041787:1,3-propanediol; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; C052370:1,3-propanediol dehydrogenase; D005990:Glycerol