[Effects of trichostatin A on the interaction between HBx and histone deacetylase protein 1].
OBJECTIVE
To study the effects of trichostatin A (TSA) on protein-protein interaction between HBx and histone deacetylase protein 1 (HDAC1).


METHODS
Both HBx and HDAC1 expressing vectors were constructed by the method of routine molecular cloning. The expression of HBx and HDAC1 were observed by Western blot assay. The protein-protein interaction was tested between HBx and HDAC1 by GST pull-down in vitro as well as co-immunoprecipitation in vivo.


RESULTS
Both HBx and HDAC1 expressing vectors were successfully constructed. Protein-protein interaction between HBx and HDAC1 existed both in vitro and in vivo. TSA, an inhibitor of HDAC1, had no effect on the interaction between HBx and HDAC1.


CONCLUSIONS
HBx interacts with HDAC1 in vivo and in vitro in a non- TSA dependent way.
Han
Ju-qiang
JQ
Institute of Hepatology, Beijing Military General Hospital, Beijing 100700, China.
Ye
Qi-nong
QN
Ding
Li-Hua
LH
Li
Jie-zhi
JZ
Yang
Xiao
X
Huang
Cui-fen
CF
D056284:Histone Deacetylase 1 / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000378:metabolism*; D047468:Immunoprecipitation; D010957:Plasmids; D025941:Protein Interaction Mapping; D015534:Trans-Activators / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins
D006877:Hydroxamic Acids; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; C012589:trichostatin A; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1