Taxol and 10-deacetylbaccatinIII induce distinct changes in the dynamics of caveolae.
Taxol treatment of HeLa cells resulted in a transient recruitment of Caveolin-1 to the cell surface followed by internalization. Interestingly, 20min after 10-deacetylbaccatinIII (10-DAB) treatment, the caveolae displayed faster 'kiss and run' dynamics while BaccatinIII (BacIII) did not induce any change. Sustained phosphorylation of Caveolin-1 is observed upon treatment and between Taxol and 10-DAB, the former shows phosphorylated Raf-1, ERK1/2 and hyperphosphorylated Bcl-2 while the later showed much less magnitude of the same. BacIII treatment did not induce phosphorylation of Raf-1 or Bcl-2. It is possible that Taxol might act on multiple targets and the side chain may be crucial.
Ahmed
Neesar
N
National Centre for Cell Science, Ganeshkhind Road, University of Pune Campus, Pune 411 007, India.
Dasari
Sreekanth
S
Srivastava
Saumya S
SS
Sneh
Amita
A
Ahmad
Absar
A
Islam Khan
M
M
Krishnasastry
M V
MV
D000970:Antineoplastic Agents / Q000494:pharmacology*; D021941:Caveolae / Q000187:drug effects* / Q000378:metabolism; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D017239:Paclitaxel / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D019908:Proto-Oncogene Proteins c-raf / Q000378:metabolism; D043823:Taxoids / Q000494:pharmacology*
D000970:Antineoplastic Agents; D051242:Caveolin 1; D043823:Taxoids; D049452:Green Fluorescent Proteins; C042213:10-deacetylbaccatine III; D019908:Proto-Oncogene Proteins c-raf; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D017239:Paclitaxel