title: Genotoxic effect of Physalis angulata L. (Solanaceae) extract on human lymphocytes treated in vitro.
abstract: Physalis angulata L (Solanaceae) is a medicinal plant from North of Brazil, whose different extracts and infusions are commonly used in the popular medicine for the treatment of malaria, asthma, hepatitis, dermatitis and rheumatism. However, the genotoxic effects of P. angulata on human cells is not well known. The main purpose of the present study was to evaluate the in vitro genotoxic effects of aqueous extract of P. angulata using the comet assay and the micronucleus assay in human lymphocytes provided from 6 healthy donors. Treatments with P. angulata extracts were performed in vitro in order to access the extent of DNA damage. The comet assay has shown that treatments with P. angulata at 0.5, 1.0, 2.0, 3.0 and 6.0 microg/mL in culture medium were genotoxic. Lymphocytes treated with P. angulata at the concentrations of 3.0 and 6.0 microg/mL in culture medium showed a statistically significant increase in the frequency of micronucleus (p<0.05), however, the cytokinesis blocked proliferation index (CBPI) was not decreased after P. angulata treatment. In conclusion, the present work demonstrated the genotoxic effects of P. angulata extract on human lymphocytes in vitro.
authors.affiliation: Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: Alves dos Santos
authors.forename: Teresinha Rosa
authors.initials: TR
authors.lastname: Cabral
authors.forename: Isabel Rosa
authors.initials: IR
authors.lastname: Cabral
authors.forename: Lusânia Maria
authors.initials: LM
authors.lastname: Antunes
authors.forename: Cristiane
authors.initials: C
authors.lastname: Pontes Andrade
authors.forename: Plínio
authors.initials: P
authors.lastname: Cerqueira dos Santos Cardoso
authors.forename: Marcelo
authors.initials: M
authors.lastname: de Oliveira Bahia
authors.forename: Claudia
authors.initials: C
authors.lastname: Pessoa
authors.forename: José Luis
authors.initials: JL
authors.lastname: Martins do Nascimento
authors.forename: Rommel
authors.initials: R
authors.lastname: Rodríguez Burbano
authors.forename: Catarina Satie
authors.initials: CS
authors.lastname: Takahashi
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D020552:Comet Assay; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D009153:Mutagens / Q000494:pharmacology*; D032321:Physalis / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity*
chemical_list: D009153:Mutagens; D010936:Plant Extracts