title: Presence of previously undescribed bacterial taxa in non-axenic Chlorella cultures.
abstract: We determined the bacterial community profile in non-axenic cultures of Chlorella (Chlorophyceae, Chlorophyta) isolated from soil. The bacterial composition at the phylum level was different from that of whole soil bacteria, but it was similar to that reported for non-axenic cultures of marine microalgae such as diatoms (Bacillariophyceae, Heterokontophyta). Expected novel bacteria, i.e. those which do not have close relatives among described species, were maintained in the cultures, and these bacteria were chiefly composed of members of the phylum Bacteroidetes. They may have been 'as-yet-uncultured' but in practice unintentionally been cultured in microalgal cultures. They could serve as good bioresources in various fields of biological and ecological studies.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. almond@mail.ecc.u-tokyo.ac.jp
authors.forename: Shigeto
authors.initials: S
authors.lastname: Otsuka
authors.forename: Yuuichi
authors.initials: Y
authors.lastname: Abe
authors.forename: Rie
authors.initials: R
authors.lastname: Fukui
authors.forename: Masaya
authors.initials: M
authors.lastname: Nishiyama
authors.forename: Keishi
authors.initials: K
authors.lastname: Sendoo
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D002708:Chlorella / Q000235:genetics / Q000302:isolation & purification / Q000382:microbiology*; D048028:DNA, Algal / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D020459:Genes, rRNA; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D048028:DNA, Algal; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S