Gold nanoparticles conjugated to [Tyr3]octreotide peptide.
A multifunctional system of gold nanoparticles (AuNP) capped by the [Tyr(3)]Octreotide (TOC) peptide was prepared and characterized by transmission electron microscopy (TEM) and UV-Vis, infrared and fluorescence spectroscopy. AuNP and AuNP-TOC fluorescence emission spectra were obtained both in solution and in murine AR42J-tumor tissues. Results suggest that AuNP were functionalized with TOC through interactions with the N-terminal amine of the phenylalanine, the amide groups and possibly with the indole group of the tryptophan residue. The fluorescence analyses in tissue revealed a recognition of the AuNP-TOC conjugate for the neuroendocrine tumor because of the lower energy position of the fluorescence resonance (692 nm) with respect to that of the AuNP in the same tumoral tissue (684 nm). The emission band observed in the near-infrared region (692 nm) opens the possibility for AuNP-TOC use in bioimaging.
Surujpaul
P P
PP
Instituto Nacional de Investigaciones Nucleares, Carretera México-Toluca S/N., La Marquesa, Ocoyoacac, Estado de México. C.P 52750, Mexico.
Gutiérrez-Wing
C
C
Ocampo-García
B
B
Ramírez
F de M
Fde M
Arteaga de Murphy
C
C
Pedraza-López
M
M
Camacho-López
M A
MA
Ferro-Flores
G
G
D000818:Animals; D045744:Cell Line, Tumor; D004339:Drug Compounding; D006046:Gold / Q000737:chemistry*; D007668:Kidney / Q000378:metabolism; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism; D015282:Octreotide / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D011485:Protein Binding; D017481:Receptors, Somatostatin / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties; D014018:Tissue Distribution
D017481:Receptors, Somatostatin; C045210:3-Tyr-octreotide; D006046:Gold; D015282:Octreotide