title: Mouse adrenal chromaffin cell isolation.
abstract: Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.
authors.affiliation: Department of Physiology and Biophysics, University of California, Irvine, CA, USA. aaron.kolski-andreaco@jove.com
authors.forename: Aaron
authors.initials: A
authors.lastname: Kolski-Andreaco
authors.forename: Haijiang
authors.initials: H
authors.lastname: Cai
authors.forename: D Spencer
authors.initials: DS
authors.lastname: Currle
authors.forename: K George
authors.initials: KG
authors.lastname: Chandy
authors.forename: Robert H
authors.initials: RH
authors.lastname: Chow
mesh_terms: D000311:Adrenal Glands / Q000166:cytology*; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D019439:Chromaffin Cells; D004210:Dissection; D051379:Mice