Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells.
BACKGROUND
RhoA and Rho kinase inhibitors overcome the inhibition of axonal regeneration posed by central nervous system (CNS) substrates.


METHODS
To investigate if inhibition of the Rho pathway augments the neurite extension that naturally occurs in the peripheral nervous system (PNS) following nerve damage, dorsal root ganglion neurons and Schwann cell co-cultures were incubated with culture medium, C3 fusion toxin, and the Rho kinase (ROCK) inhibitors Y27632 and H1152. The longest neurite per neuron were measured and compared. Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells. When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites. This work demonstrates that Rho kinase inhibition augments neurite elongation in the presence of contact with a PNS-like substrate.
Fuentes
Erick O
EO
Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Freiburg, Germany. ckitofdr@gmail.com
Leemhuis
Jost
J
Stark
G Björn
GB
Lang
Eva M
EM