title: Three-dimensional ingrowth of bone cells within biodegradable cryogel scaffolds in bioreactors at different regimes.
abstract: Three-dimensional cell ingrowth within biodegradable cryogel scaffolds made of cross-linked 2-hydroxyethyl methacrylate (HEMA)-lactate-dextran with interconnected macropores was studied in bioreactors at different regimes (static, perfusion, and compression-perfusion). An osteoblast-like cell line (MG63) was used in these studies. The samples taken after selected times from the bioreactors were examined by microscopy techniques (light, SEM, TEM, and laser scanning confocal). The cell culture conditions were found to have a significant impact not only on the cell morphology, such as the extent of cell attachment and ingrowth, but also on cellular activities. Dynamic conditions (perfusion and/or compression) greatly improved cell ingrowth and extracellular matrix (ECM) synthesis. Alkaline phosphatase activity results confirmed the positive effect of dynamic conditions on bone cells.
authors.affiliation: Bioengineering Division, Chemical Engineering Department, Hacettepe University, Ankara, Turkey.
authors.forename: Nimet
authors.initials: N
authors.lastname: Bölgen
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Peter
authors.initials: P
authors.lastname: Korkusuz
authors.forename: Elif
authors.initials: E
authors.lastname: Güzel
authors.forename: Alicia J
authors.initials: AJ
authors.lastname: El Haj
authors.forename: Erhan
authors.initials: E
authors.lastname: Pişkin
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D019149:Bioreactors; D001798:Blood Proteins / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D059985:Cryogels; D003911:Dextrans / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels; D007773:Lactates / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D059985:Cryogels; D003911:Dextrans; D005353:Fibronectins; D020100:Hydrogels; D007773:Lactates; D008689:Methacrylates; D011108:Polymers; C005044:hydroxyethyl methacrylate