Photodynamic activity of C70 caged within surface-cross-linked liposomes.
[70]Fullerene (C(70)) encapsulated into a surface-cross-linked liposome, a so-called cerasome, was prepared by an exchange reaction incorporating C(70)gamma-cyclodextrin complexes into lipid membranes. Fullerene exchange in a cerasome-incorporated C(70) (CIC(70)), as well as in a lipid-membrane-incorporated C(70) (LMIC(70)), was completed within 1 min with stirring at 25 degrees C. CIC(70) was more resistant to lysis than LMIC(70) towards lysing agents such as surfactants. Furthermore, the photodynamic activity of CIC(70) in HeLa cells was similar to that of LMIC(70), indicating that C(70) can act as a photosensitizing drug (PS) without release from cerasome membranes. Thus, in contrast with general drug-delivery systems (DDSs), which require the drug to be released from the interior of liposomes, carriers for PSs for use in photodynamic therapy (PDT) do not necessarily need to release the drug. These results indicate that DDSs with high morphological stability can increase the residence time in blood and achieves tumor-selective drug delivery by the enhanced permeability and retention (EPR) effect.
Ikeda
Atsushi
A
Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan. aikeda@ms.naist.jp
Nagano
Mai
M
Akiyama
Motofusa
M
Matsumoto
Masashi
M
Ito
Sayuri
S
Mukai
Masaru
M
Hashizume
Mineo
M
Kikuchi
Jun-ichi
J
Katagiri
Kiyofumi
K
Ogawa
Takuya
T
Takeya
Tatsuo
T
D003505:Cyclodextrins / Q000737:chemistry; D016503:Drug Delivery Systems; D004705:Endocytosis; D037741:Fullerenes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size; D010539:Permeability; D010778:Photochemotherapy; D012833:Siloxanes / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D003505:Cyclodextrins; D037741:Fullerenes; D008081:Liposomes; D012833:Siloxanes; C069836:fullerene C70