title: The mechanism of acidic hydrolysis of esters explains the HDV ribozyme activity.
abstract: The hepatitis delta virus (HDV) ribozyme is an RNA enzyme that catalyzes the site-specific trans-esterification reaction. Using high hydrostatic pressure (HHP) technique we showed that HDV ribozyme catalyzes the reaction of RNA cleavage in the absence of magnesium ions according to mechanism of acidic hydrolysis of esters. HHP induces changes of water structure, lowering pH and effect ribozyme catalytic site structure formation without magnesium. HHP, similarly to magnesium ion at ambient pressure stabilizes the higher order RNA structure of HDV, but Mg(2+) is not involved in the catalysis. Our results clearly support the new mechanism of HDV hydrolysis and show advantages of using HHP in analysis of macromolecules interaction.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Fedoruk-Wyszomirska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Giel-Pietraszuk
authors.forename: Eliza
authors.initials: E
authors.lastname: Wyszko
authors.forename: Maciej
authors.initials: M
authors.lastname: Szymański
authors.forename: Jerzy
authors.initials: J
authors.lastname: Ciesiołka
authors.forename: Mirosława Z
authors.initials: MZ
authors.lastname: Barciszewska
authors.forename: Jan
authors.initials: J
authors.lastname: Barciszewski
mesh_terms: D000143:Acids / Q000378:metabolism*; D001483:Base Sequence; D055162:Biocatalysis; D004952:Esters / Q000378:metabolism*; D016679:Genome, Viral / Q000235:genetics; D003698:Hepatitis Delta Virus / Q000201:enzymology*; D006868:Hydrolysis; D006874:Hydrostatic Pressure; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000143:Acids; D004952:Esters; D016337:RNA, Catalytic