title: Immunomodulatory activity of methanolic extract of Murraya koenigii (L) Spreng. leaves.
abstract: Immunomodulatory activity of methanolic extract of M. koenigii leaves was evaluated on humoral and cell mediated immune response to ovalbumin, phagocytic activity by carbon clearance test, nitric oxide (NO) release from murine peritoneal macrophages and cyclophosphamide induced myelosuppression. Significant increase in the NO production by mouse peritoneal macrophages was detected in culture supernatants indicated increased phagocytic activity of macrophages. The extract showed significant increase in phagocytic index by rapid removal of carbon particles from blood stream. The extract also increased the antibody titre against the ovalbumin and protection towards the cyclophosphamide induced myelosuppression. However, the extract did not show any significant increase in delayed type hypersensitivity reaction which indicated the inability of the extract to stimulate T cells. Present study thus reveals that the extract holds promise as immunomodulatory agent, which acts by stimulating humoral immunity and phagocytic function.
authors.affiliation: Department of Pharmaceutical Sciences and Technology, Mumbai University Institute of Chemical Technology Nathalal Parikh Marg, Matunga, Mumbai 400 019, India.
authors.forename: Abhishek S
authors.initials: AS
authors.lastname: Shah
authors.forename: Alok S
authors.initials: AS
authors.lastname: Wakade
authors.forename: Archana R
authors.initials: AR
authors.lastname: Juvekar
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D007109:Immunity / Q000187:drug effects* / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D000432:Methanol; D051379:Mice; D032101:Murraya / Q000737:chemistry*; D009573:Nitrites / Q000378:metabolism; D010586:Phagocytes / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D009573:Nitrites; D010936:Plant Extracts; D000432:Methanol