A comparative study of inhibitory effect of human calprotectin on the growth of human gingival fibroblast and foreskin fibroblast.
In the present study, cytotoxicity effects of calprotectin on Human Gingival Fibroblast (HGF) and Human Foreskin Fibroblast (HFFF) were compared. For these evaluations, both cells were exposed to the different concentrations of calprotectin, for 24, 48 and 72 h. Cell proliferation was assessed using MTT assay. Our results revealed that growth inhibition of calprotectin on HGF and HFFF occur in a dose- and time-dependent manner. Results of this investigation showed that sensitivity of HGF cells to cytotoxic effect of human calprotectin was more than HFFF. The results indicate that drug resistance process is different for the two kinds of fibroblast cells.
Rezaei-Tavirani
Mostafa
M
Medical Proteomics Research Center, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
Zali
Hakimeh
H
Nabai
Layla
L
Toossi
Parviz
P
Zad
Farhad Malek
FM
Yousof
Pourkhoshbakht
P
D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism*; D052816:Foreskin / Q000378:metabolism*; D005881:Gingiva / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D039841:Leukocyte L1 Antigen Complex / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D009504:Neutrophils / Q000378:metabolism; D012680:Sensitivity and Specificity; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors
D039841:Leukocyte L1 Antigen Complex; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue