[The mechanism of resveratrol on anti-hepatoma Bel-7402 and modulating IL-8 in tumor model mice].
OBJECTIVE
To explore the anti-tumor effect of resveratrol on the hepatocellular carcinoma cell line Bel-7402 both in vitro and in vivo.


METHODS
MTT colorimetry was used to measure the tumor cell growth repression rate. The level of Bcl-2 was measured by Western blotting. The cell cycle of Bel-7402 and intracellular IL-8 were analyzed by flow cytometry. The expression of IL-8 mRNA was determined by semiquantitative RT-PCR and quantification assay of IL-8 was determined by ELISA.


RESULTS
Our findings indicated that resveratrol inhibited proliferation, induced apoptosis, and influenced cell cycle of Bel-7402 cells at a dose-and time-dependent manner in vitro. Furthermore, resveratrol could exert a dose-related down-regulatory effect on IL-8 in Bel-7402 bearing mice.


CONCLUSIONS
It suggestes resveratrol might have chemotherapeutic potential and immunomodulation action to Bel-7402 to cellular carcinoma both in vitro and in vivo.
Li
Tan
T
Department of Immunology, Medical College of the Chinese People's Armed Police Force, Tianjin 300162, China. litan2002@eyou.com
Wang
Wei
W
Li
Tong
T
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005434:Flow Cytometry; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D016209:Interleukin-8; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D013267:Stilbenes; D000077185:Resveratrol