Effects of 2-deoxy-D-glucose on proliferation of vascular smooth muscle cells and endothelial cells.
2-Deoxy-D-glucose (2-DG) is a glucose analogue that has been proposed for cancer therapy due to its cytostatic properties. Its effect on the proliferation of smooth muscle cells and endothelial cells has not been fully clarified. The aims of this study were to investigate the effects of 2-DG on the proliferation of porcine aortic endothelial cells (PAEC) and porcine smooth muscle cells (PSMC), to establish an overview of its dose-dependent inhibitory capacity and to examine whether the short-term incubation of cells with 2-DG has an impact on cell proliferation in culture. Our results showed a dose-dependent significant inhibitory effect on proliferation, which was more pronounced in PSMC than in PAEC. Even after short-term incubation of cells with 2-DG, relevant inhibition of proliferation was documented. The clinical application of 2-DG might be a promising concept by inhibiting cells that show a potentially rapid proliferation in response to non-malignant stimuli, such as smooth muscle cells after intracoronary stenting.
Nef
H M
HM
Kerckhoff Heart Centre, Department of Cardiology, Bad Nauheim, Germany. holger.nef@mpi-bn.mpg.de
Möllmann
H
H
Joseph
A
A
Troidl
C
C
Voss
S
S
Vogt
A
A
Weber
M
M
Hamm
C W
CW
Elsässer
A
A
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology*; D001011:Aorta / Q000166:cytology; D049109:Cell Proliferation / Q000187:drug effects*; D003847:Deoxyglucose / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000502:physiology; D013552:Swine
D000963:Antimetabolites; D003847:Deoxyglucose