Interaction of grape ASR proteins with a DREB transcription factor in the nucleus.
ASR proteins (abscissic acid, stress, ripening induced) are involved in plant responses to developmental and environmental signals but their biological functions remain to be elucidated. Grape ASR gene (VvMSA) encodes a new transcription factor regulating the expression of a glucose transporter. Here, we provide evidence for some polymorphism of grape ASRs and their identification as chromosomal non-histone proteins. By the yeast two-hybrid approach, a protein partner of VvMSA is isolated and characterized as an APETALA2 domain transcription factor. Interaction of the two proteins is further demonstrated by the BiFC approach and the exclusive nuclear localization of the heterodimer is visualized.
Saumonneau
Amélie
A
Université de Poitiers, FRE CNRS 3091 "Physiologie moléculaire du transport des sucres", Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
Agasse
Alice
A
Bidoyen
Marie-Thérèse
MT
Lallemand
Magali
M
Cantereau
Anne
A
Medici
Anna
A
Laloi
Maryse
M
Atanassova
Rossitza
R
D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D019281:Dimerization; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D027843:Vitis / Q000235:genetics / Q000378:metabolism*
D009687:Nuclear Proteins; D010940:Plant Proteins; D014157:Transcription Factors