Regulation of the expression of GA-insensitive gene homolog by light in Oryza sativa c.v. DongJin.
To understand the mechanism by which light regulates a gibberellin (GA)-insensitive gene in DongJinByeo (Oryza sativa cv DongJin), both green and etiolated DongJinByeo seedlings were submerged in water and treated with GA. Total RNA from the seedlings was isolated and hybridized with cDNA of a GA-insensitive gene homolog. The amount of transcript for the GA-insensitive gene homolog was higher in green seedlings than in etiolated seedlings in the absence of GA. However upon the addition of GA, greater accumulations of the gene transcript occurred in etiolated seedlings than in green seedlings. This result indicates the possibility that the expression of the GA-insensitive gene homolog transcript may be inhibited by light in the presence of GA. Light seems to regulate multilaterally the accumulation of the transcript of the GA-insensitive gene homolog in DongJinByeo (Oryza sativa cv DongJin).
Kim
Jae Hong
JH
Department of Biotechnology, Soonchunhyang University, Asan PO Box 97, Chungnam 336 600, Korea.
Lee
Mi Young
MY
D015152:Blotting, Northern; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D005875:Gibberellins; D008027:Light; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000145:classification / Q000235:genetics* / Q000528:radiation effects*; D036226:Seedlings / Q000235:genetics / Q000528:radiation effects; D013997:Time Factors
D018076:DNA, Complementary; D005875:Gibberellins