[Phenotypic and genotypic identification of bacteria from the Burkholderia cepacia complex].
OBJECTIVE
Evaluation of the diagnostic value of pheno- and genotypic characteristics of B. cepacia strains collection.


METHODS
Phenotypic and genetic methods of identification and differentiation of 25 strains of the B. cepacia complex.


RESULTS
Polyphasic taxonomic approach utilizing multiple diagnostic tests was used for accurate identification of Burkholderia species. Algorithm for identification of microorganisms from the B. cepacia complex was developed.


CONCLUSIONS
Combined use of phenotypic and molecular genetic tests, such as recA gene PCR, is recommended for differentiation of the B. cepacia complex genomovars.
Antonov
V A
VA
Iliukhin
V I
VI
Senina
T V
TV
Loboĭko
A D
AD
Tkachenko
G A
GA
Zinchenko
O V
OV
Mazurova
I Iu
IIu
D000465:Algorithms; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D019121:Burkholderia Infections / Q000175:diagnosis* / Q000382:microbiology; D042602:Burkholderia cepacia complex / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D006801:Humans; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011938:Rec A Recombinases / Q000235:genetics
D001426:Bacterial Proteins; D011938:Rec A Recombinases