title: Comparative study of the effects of gentamicin, neomycin, streptomycin and ofloxacin antibiotics on sperm parameters and testis apoptosis in rats.
abstract: The aim of this study was to investigate the comparative effects of aminoglycosides and fluoroquinolones on testis apoptosis and sperm parameters in rats. Fifty male Wistar rats were randomly divided into control (n = 10) and experimental (n = 40) groups. The experimental groups subdivided into four groups often. Each received 5 mg kg(-1) (IP) gentamicin, 50 mg kg(-1) (IP) neomycin, 40 mg kg(-1) (IP) streptomycin and 72 mg kg(-1) (IP) ofloxacin daily for 14 days, respectively; however, the control group just received vehicle (IP). In the fourteenth day, rats were killed and sperm analyzed for sperm parameters. Testis tissues were also prepared for TUNEL assay for detection of apoptosis. There was a significant decrease in sperm count, viability and motility in all of experimental groups when compared with control group. Although in streptomycin group these parameters were less decreased than in the other experimental groups. The apoptotic cells were significantly increased in all experimental groups when compared with those seen in the controlled group. Gentamicin, neomycin and streptomycin and ofloxacin have negative effects on sperm parameters and testis apoptosis in rats. However, these side effects are less seen in the streptomycin group. Therefore, it is recommended that usage of this drug have fewer side effects on male fertility.
authors.affiliation: Department of Veterinary Pathology, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Khaki
authors.forename: Marefat Ghaffari
authors.initials: MG
authors.lastname: Novin
authors.forename: Amir Afshin
authors.initials: AA
authors.lastname: Khaki
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nouri
authors.forename: Ehsan
authors.initials: E
authors.lastname: Sanati
authors.forename: Mahdad
authors.initials: M
authors.lastname: Nikmanesh
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D005839:Gentamicins / Q000494:pharmacology; D008297:Male; D009355:Neomycin / Q000494:pharmacology; D015242:Ofloxacin / Q000494:pharmacology; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects*; D013307:Streptomycin / Q000494:pharmacology; D013737:Testis / Q000166:cytology* / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D015242:Ofloxacin; D009355:Neomycin; D013307:Streptomycin