Glycoarrays--tools for determining protein-carbohydrate interactions and glycoenzyme specificity.
Carbohydrate arrays (glycoarrays) have recently emerged as a high-throughput tool for studying carbohydrate-binding proteins and carbohydrate-processing enzymes. A number of sophisticated array platforms that allow for qualitative and quantitative analysis of carbohydrate binding and modification on the array surface have been developed, including analysis by fluorescence spectroscopy, mass spectrometry and surface plasmon resonance spectroscopy. These platforms, together with examples of biologically-relevant applications are reviewed in this Feature Article.
Laurent
Nicolas
N
Manchester Interdisciplinary Biocentre and School of Chemistry, The University of Manchester, 131 Princess Street, Manchester, UK M1 7DN.
Voglmeir
Josef
J
Flitsch
Sabine L
SL
D002241:Carbohydrates / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry*; D013379:Substrate Specificity
D002241:Carbohydrates; D004798:Enzymes; D011506:Proteins