Analysis of urinary andrographolides and antioxidant status after oral administration of Andrographis paniculata leaf extract in rats.
A simple high-performance liquid chromatography (HPLC) method was developed to determine the content of andrographolide (AP) and 14-deoxy-11,12-dideoxyandrographolide (DIAP) in a pooled urine of rat obtained within 24h after an oral dose of Andrographis paniculata leaf extract at 1g/kg body weight. Cumulative urinary excretion of AP and DIAP in 24h after oral administration of the extract was 0.88% and 1.61% of oral dose administered, respectively. The extract showed significant reduction (p<0.05) of MDA levels and elevation of total antioxidant status in rat urine samples collected in 24 after oral administration.
Akowuah
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GA
University College Sedaya International, Taman Connaught, 56000 Cheras, Kuala Lumpur, Malaysia. wuahmy@yahoo.com
Zhari
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I
Mariam
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A
D000284:Administration, Oral; D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000652:urine*; D015227:Lipid Peroxidation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results
D000975:Antioxidants; D004224:Diterpenes; D010936:Plant Extracts; C030419:andrographolide