In vitro and in vivo validation of ligA and tarI as essential targets in Staphylococcus aureus.
A conditional expression system has been developed using the isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible Pspac promoter to validate essential genes of Staphylococcus aureus in vivo. The system has been applied to prove the essentiality of ligA and to evaluate the function of tarI, which was found to be essential in vitro but not in vivo.
Streker
Karin
K
Universität Würzburg, Institut für Molekulare Infektionsbiologie, Röntgenring 11, 97070 Würzburg, Germany.
Schäfer
Tina
T
Freiberg
Christoph
C
Brötz-Oesterhelt
Heike
H
Hacker
Jörg
J
Labischinski
Harald
H
Ohlsen
Knut
K
D000038:Abscess / Q000382:microbiology; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D003470:Culture Media; D011088:DNA Ligases / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D020043:Genes, Essential; D006801:Humans; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D051379:Mice; D009154:Mutation; D013207:Staphylococcal Skin Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*
D001426:Bacterial Proteins; D003470:Culture Media; D007544:Isopropyl Thiogalactoside; D011088:DNA Ligases