Cross-species utility of microsatellite markers in trichostrongyloid nematodes.
The development of microsatellite markers for parasitic nematodes has been hampered by technical difficulties in isolation and PCR amplification. We have investigated the potential for circumventing these problems using microsatellites from 3 trichostrongyloid species on a panel of 7 species. Ten of the 22 PCR primer pairs tested amplified in species other than the target species, usually in closely related species, and 2 new variable loci were discovered in the sheep parasite Trichostrongylus vitrinus. This study provides evidence that cross-species testing of microsatellite primers can be an effective alternative to isolation de novo.
Temperley
Nicholas D
ND
Division of Genetics and Genomics, Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, UK. nick.temperley@bbsrc.ac.uk
Webster
Lucy M I
LM
Adam
Aileen
A
Keller
Lukas F
LF
Johnson
Paul C D
PC
D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry; D014644:Genetic Variation; D008297:Male; D018895:Microsatellite Repeats / Q000502:physiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012756:Sheep; D014251:Trichostrongyloidea / Q000145:classification / Q000235:genetics*; D014254:Trichostrongylus / Q000145:classification / Q000235:genetics
D018509:DNA, Helminth