[Effect of garlicin on the serum levels of interleukin 4 and interferon gamma in allergic rhinitis model in rats].
OBJECTIVE
To investigate the effect of garlicin on the levels of interferon gamma (INF-gamma) and interleukin 4 (IL-4) in blood of allergic rhinitis rat model.


METHODS
Thirty healthy female SD rats were randomly divided into 3 groups: control group, negative control group and experimental group, 10 rats for each group. Ten rats (experimental group) were sensitized and intranasally challenged by ovalbumin, aluminium hydroxide hydrate gel and Bordetella pertussis inactive microorganism suspension adjuvants, as allergic rhinitis models, and then injection of garlicin(0.4 ml) intraperitoneally per day for 10 days. Control group rats were immunized as experimental group, and then injection of physiological saline as equal volume as garlicin. Negative control group rats were investigated using physiological saline. Blood of intrajugular vein of rat was extracted for separated plasma Enzyme liked immunosorbent assay (ELISA) was utilized to detect the serum levels of IL-4 and IFN-gamma.


RESULTS
The serum levels (x +/- s) of IL4 were (22.81 +/- 8.79) pg/L, (41.43 +/- 4.93) pg/L, (9.93 +/- 2.07) pg/L, and those of IFN-gamma were (22.32 +/- 11.20) pg/L, (11.35 +/- 2.45) pg/L and (21.69 +/- 5.93) pg/L, respectively, among experimental group, control group and negative control group. The serum level of IL-4 in experimental group rats was lower than value of control group rats (t = 3.22, P < 0.05), while higher than negative control group (t = 4.17, P < 0.05). The serum level of IFN-gamma was increased significantly in experimental group rats with significant difference when compared with value of control group rats (t = 3.84, P < 0.05), while no difference was shown between experimental group and negative control group (t = 1.47, P > 0.05).


CONCLUSIONS
Garlicin could increase serum level of INF-gamma and decrease serum level of IL4 significantly in allergic rhinitis rat model. It played an important role on regulating serum levels of cytokines of Thl and Th2.
Li
Yu-Xiao
YX
Otorhinolarygology Hospital, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
Chen
Dong
D
Li
Tian-Ying
TY
Feng
Lian-Qiang
LQ
Xu
Geng
G
Wen
Wei-Ping
WP
D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D004195:Disease Models, Animal; D004220:Disulfides / Q000494:pharmacology*; D005260:Female; D007371:Interferon-gamma / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D006255:Rhinitis, Allergic, Seasonal / Q000097:blood*; D018417:Th1 Cells / Q000187:drug effects; D018418:Th2 Cells / Q000187:drug effects
D000498:Allyl Compounds; D004220:Disulfides; D015847:Interleukin-4; C028009:diallyl disulfide; D007371:Interferon-gamma