In vitro pharmacogenomic database and chemosensitivity predictive genes in gastric cancer.
Gastric cancer is one of the most common cancers worldwide, and there are clinical caveats in predicting tumor response to chemotherapy. This study describes the construction of an in vitro pharmacogenomic database, and the selection of genes associated with chemosensitivity in gastric cancer cell lines. Gene expression and chemosensitivity databases were integrated using the Pearson correlation coefficient to give the GC-matrix. The 85 genes were selected that were commonly associated with chemosensitivity of the major anticancer drugs. We then focused on the genes that were highly correlated with each specific drug. Classification of cell lines based on the set of genes associated with each drug was consistent with the division into resistant or sensitive groups according to the chemosensitivity results. The GC-matrix of the gastric cancer cell line database was used to identify different sets of chemosensitivity-related genes for specific drugs or multiple drugs.
Jung
Jae-Joon
JJ
Cancer Metastasis Research Center, Yonsei University College of Medicine, Seoul, 120-752, Korea.
Jeung
Hei-Cheul
HC
Chung
Hyun Cheol
HC
Lee
Jung Ok
JO
Kim
Tae Soo
TS
Kim
Yong Tai
YT
Noh
Sung Hoon
SH
Rha
Sun Young
SY
D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D030541:Databases, Genetic; D019008:Drug Resistance, Neoplasm; D020869:Gene Expression Profiling; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011237:Predictive Value of Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics*
D000970:Antineoplastic Agents; D009363:Neoplasm Proteins