A SEC-HPLC-ICP MS hyphenated technique for identification of sulfur-containing arsenic metabolites in biological samples.
The present study describes the synthesis and characterization of thioarsenicals using electro-spray ionization-MS and time of flight-MS. Separation of thioarsenicals was found to be better by size-exclusion column compared to anion exchange column coupled with HPLC-inductively coupled argon plasma mass spectrometer (ICP MS). Although four thioarsenicals were confirmed as dimethylthioarsinous acid (m/z=138), methylmonothioarsonous acid (m/z=122), dimethyldithioarsinic acid (m/z=170) and methyltrithioarsonic acid (m/z=188), it is noted that HPLC-ICP MS alone were not sufficient for their identification. Also, none of them was stable with time. This is the first report detailing the synthesis and identification of methyltrithioarsonic acid. Both dimethyldithioarsinic acid and dimethylthioarsinous acid were detected in human nail samples while dimethyldithioarsinic acid was found in urine samples. So, the above technique could be applicable to the identification of sulfur-containing biomolecules in the biological samples.
Mandal
Badal Kumar
BK
Environmental and Analytical Chemistry Division, School of Science and Humanities, VIT University, Vellore 632014, India. badalmandal@vit.ac.in
Suzuki
Kazuo T
KT
Anzai
Kazunori
K
Yamaguchi
Kentaro
K
Sei
Yoshihisa
Y
D001152:Arsenicals / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D002101:Cacodylic Acid / Q000031:analogs & derivatives / Q000378:metabolism / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013455:Sulfur / Q000737:chemistry*
D001152:Arsenicals; C518668:dimethyldithioarsinic acid; D013455:Sulfur; D002101:Cacodylic Acid