title: A physical map of the 1-gigabase bread wheat chromosome 3B.
abstract: As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.
authors.affiliation: Institut National de la Recherche Agronomique, Université Blaise Pascal (INRA-UBP), UMR 1095, Genetics Diversity and Ecophysiology of Cereals, Clermont-Ferrand, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Paux
authors.forename: Pierre
authors.initials: P
authors.lastname: Sourdille
authors.forename: Jérôme
authors.initials: J
authors.lastname: Salse
authors.forename: Cyrille
authors.initials: C
authors.lastname: Saintenac
authors.forename: Frédéric
authors.initials: F
authors.lastname: Choulet
authors.forename: Philippe
authors.initials: P
authors.lastname: Leroy
authors.forename: Abraham
authors.initials: A
authors.lastname: Korol
authors.forename: Monika
authors.initials: M
authors.lastname: Michalak
authors.forename: Shahryar
authors.initials: S
authors.lastname: Kianian
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Spielmeyer
authors.forename: Evans
authors.initials: E
authors.lastname: Lagudah
authors.forename: Daryl
authors.initials: D
authors.lastname: Somers
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kilian
authors.forename: Michael
authors.initials: M
authors.lastname: Alaux
authors.forename: Sonia
authors.initials: S
authors.lastname: Vautrin
authors.forename: Hélène
authors.initials: H
authors.lastname: Bergès
authors.forename: Kellye
authors.initials: K
authors.lastname: Eversole
authors.forename: Rudi
authors.initials: R
authors.lastname: Appels
authors.forename: Jan
authors.initials: J
authors.lastname: Safar
authors.forename: Hana
authors.initials: H
authors.lastname: Simkova
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Dolezel
authors.forename: Michel
authors.initials: M
authors.lastname: Bernard
authors.forename: Catherine
authors.initials: C
authors.lastname: Feuillet
mesh_terms: D001939:Bread; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics*; D020451:Contig Mapping; D016172:DNA Fingerprinting; D020224:Expressed Sequence Tags; D015723:Gene Library; D018745:Genome, Plant; D018895:Microsatellite Repeats; D012275:Oryza / Q000235:genetics; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic; D011123:Polyploidy; D021101:Radiation Hybrid Mapping; D014908:Triticum / Q000235:genetics*