title: Muscimol dialysis into the caudal aspect of the Nucleus tractus solitarii of conscious rats inhibits chemoreception.
abstract: We studied the effects on chemoreception of bilateral focal inhibition of the caudal Nucleus tractus solitarii (cNTS) by microdialysis of muscimol (0.5 mM) in rats during wakefulness and NREM sleep at two temperatures, 24 degrees C and 30 degrees C, just below and within the thermoneutral zone, respectively. Body temperature and VO2 did not differ at these two temperatures. The CO2 response (% increase in V(E)/VO2) did not differ at 24 degrees C vs. 30 degrees C and muscimol inhibited the CO2 response equally at both temperatures. In contrast, the hypoxic response (% increase in V(E)/VO2) was greater at 30 degrees C than at 24 degrees C and muscimol inhibited it only at 30 degrees C. These effects were similar in wakefulness and NREM sleep. We conclude that: (1) ambient temperature can affect the V(E)/VO2 response to hypoxia but not hypercapnia and (2) at 24 degrees C muscimol in the cNTS affects the CO2 response but not the hypoxic response providing indirect support for the presence of chemoreception within the NTS.
authors.affiliation: Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, United States. Eugene.nattie@dartmouth.edu
authors.forename: Eugene
authors.initials: E
authors.lastname: Nattie
authors.forename: Aihua
authors.initials: A
authors.lastname: Li
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology; D002245:Carbon Dioxide / Q000494:pharmacology; D003956:Dialysis / Q000379:methods; D004569:Electroencephalography; D004576:Electromyography; D018755:GABA Agonists / Q000494:pharmacology*; D000860:Hypoxia / Q000503:physiopathology; D008297:Male; D009118:Muscimol / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D012123:Pulmonary Ventilation / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep / Q000187:drug effects / Q000502:physiology; D017552:Solitary Nucleus / Q000187:drug effects*; D014851:Wakefulness / Q000187:drug effects / Q000502:physiology
chemical_list: D018755:GABA Agonists; D002245:Carbon Dioxide; D009118:Muscimol