[Construction of a stable genetically engineered microorganism for degrading HCH & methyl parathion and its characteristics].
A GEM designated as BHC-A-mpd, capable of simultaneously degrading of methyl parathion (MP) and HCH was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into chromosome of a HCH-degrading strain BHC-A with the mini-Tn-transposon system. The growth and degrading characteristics of BHC-A-mpd was compared with the original strain BHC-A, and the result showed that there was no difference in this two aspects, A600 nm of BHC-A-mpd in LB medium could reach 2.5 in logarithmic period, which was the same as that of the original strain BHC-A.BHC-A-mpd showed the same HCH-degrading ability as BHC-A and could degrade 5 mg/L of gamma-HCH in 10 h. BHC-A-mpd showed high genetica stability and could degrade many kinds of organophosphorus pesticides. All these results indicated that BHC-A-mpd was a promising GEM in bioremediation of MP and HCH co-contaminated environment.
Lu
Peng
P
Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China.
Hong
Yuan-Fan
YF
Hong
Qing
Q
Jiang
Xin
X
Li
Shun-Peng
SP
D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D004785:Environmental Pollutants / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods; D001556:Hexachlorocyclohexane / Q000737:chemistry / Q000378:metabolism*; D008743:Methyl Parathion / Q000737:chemistry / Q000378:metabolism*
D004785:Environmental Pollutants; D008743:Methyl Parathion; D001556:Hexachlorocyclohexane