title: Sex- and depot-specific lipolysis regulation in human adipocytes: interplay between adrenergic stimulation and glucocorticoids.
abstract: The purpose of this investigation was to explore interactions between adrenergic stimulation, glucocorticoids, and insulin on the lipolytic rate in isolated human adipocytes from subcutaneous and omental fat depots, and to address possible sex differences. Fat biopsies were obtained from 48 nondiabetic subjects undergoing elective abdominal surgery. Lipolysis rate was measured as glycerol release from isolated cells and proteins involved in lipolysis regulation were assessed by immunoblots. Fasting blood samples were obtained and metabolic and inflammatory variables were analyzed. In women, the rate of 8-bromo-cAMP- and isoprenaline-stimulated lipolysis was approximately 2- and 1.5-fold higher, respectively, in subcutaneous compared to omental adipocytes, whereas there was no difference between the two depots in men. Dexamethasone treatment increased the ability of 8-bromo-cAMP to stimulate lipolysis in the subcutaneous depot in women, but had no consistent effects in fat cells from men. Protein kinase A, Perilipin A, and hormone sensitive lipase content in adipocytes was not affected by adipose depot, sex, or glucocorticoid treatment. In conclusion, catecholamine and glucocorticoid regulation of lipolysis in isolated human adipocytes differs between adipose tissue depots and also between sexes. These findings may be of relevance for the interaction between endogenous stress hormones and adipose tissue function in visceral adiposity and the metabolic syndrome.
authors.affiliation: Department of Medicine, Umeå University Hospital, Umeå, Sweden. magdalena.lundgren@medicin.umu.se
authors.forename: M
authors.initials: M
authors.lastname: Lundgren
authors.forename: J
authors.initials: J
authors.lastname: Burén
authors.forename: P
authors.initials: P
authors.lastname: Lindgren
authors.forename: T
authors.initials: T
authors.lastname: Myrnäs
authors.forename: T
authors.initials: T
authors.lastname: Ruge
authors.forename: J W
authors.initials: JW
authors.lastname: Eriksson
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D017667:Adipocytes / Q000378:metabolism*; D000293:Adolescent; D000322:Adrenergic Agonists / Q000494:pharmacology*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002352:Carrier Proteins; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis / Q000502:physiology*; D008297:Male; D024821:Metabolic Syndrome / Q000378:metabolism; D008875:Middle Aged; D000070778:Perilipin-1; D010529:Peritoneal Cavity / Q000166:cytology; D010750:Phosphoproteins / Q000378:metabolism; D012727:Sex Characteristics; D002787:Sterol Esterase / Q000378:metabolism; D050151:Subcutaneous Fat / Q000166:cytology; D055815:Young Adult
chemical_list: D000322:Adrenergic Agonists; D000318:Adrenergic beta-Agonists; D002352:Carrier Proteins; D005938:Glucocorticoids; D000070778:Perilipin-1; D010750:Phosphoproteins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D003907:Dexamethasone; D017868:Cyclic AMP-Dependent Protein Kinases; D002787:Sterol Esterase; D007545:Isoproterenol