Chemical constituents isolated from Polygala japonica leaves and their inhibitory effect on nitric oxide production in vitro.
A methanolic extract of dried leaves of Polygala japonica Houtt (Polygalaceae) significantly attenuated nitric oxide production in lipopolysaccharide-simulated BV2 microglia. Five anthraquinones chrysophanol (1), emodin (2), aloe-emodin (3), emodin 8-O-beta-D-glucopyranoside (4) and trihydroxy anthraquinone (5), and four flavonoids kaempferol (6), chrysoeriol (7), kaempferol 3-gentiobioside (8) and isorhamnetin (9) were isolated from the methanolic extract using bioactivity-guided fractionation. Among them, compounds 1-4, 6 and 7 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglia at the concentrations ranging from 1.0 to 100.0 microM.
Kim
Seung Hyun
SH
Institute for Life Science, ElcomScience Co. Ltd., Seoul, Republic of Korea.
Jang
Sin-Duk
SD
Lee
Ki Yong
KY
Sung
Sang Hyun
SH
Kim
Young Choong
YC
D000880:Anthraquinones / Q000302:isolation & purification / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D017628:Microglia; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D031801:Polygala / Q000737:chemistry*
D000880:Anthraquinones; D005419:Flavonoids; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide