Preventive role of magnesium on skeletal muscle ischemia-reperfusion injury-an experimental study.
The present study aims to explore whether Mg infusion has a preventive effect on ischemia-reperfusion injury in rats. A total of 20 Sprague-Dawley-type adult male rats were used. In group 1 (control), 0.9% isotonic solution was administered. In group 2 (experiment), magnesium sulfate (0.5 mg per 100 g) was administered. Ischemia was induced for 15 min for the two groups. Magnesium (Mg), interleukin 8 (IL-8), and malondialdehyde levels were analyzed in blood, while edema, neutrophil infiltration, eosinophilia, loss of striation, and nucleolization were evaluated in histopathological examination. Mg levels in the experiment group were higher than those in the control group after ischemia-reperfusion (p < 0.05). In the control group, postischemia and postreperfusion IL-8 values were higher than preoperative values (p < 0.05). As for eosinophilia and loss of striation values, these were higher in the experiment group after ischemia-reperfusion than the values in the control group (p < 0.05). Histopathologically, Mg infusion cannot prevent the tissue injury triggered in ischemia-reperfusion periods. Eosinophilia can be one of the major and earliest markers of ischemia-reperfusion injury.
Gormus
Zulfikare Isik
ZI
Department of Physiology, Meram Medical School, Selcuk University, 42080, Konya, Turkey.
Ergene
Neyhan
N
Toy
Hatice
H
Baltaci
Abdulkerim Kasim
AK
Mogulkoc
Rasim
R
D000818:Animals; D004487:Edema / Q000473:pathology; D004802:Eosinophilia / Q000473:pathology; D016209:Interleukin-8 / Q000097:blood; D008274:Magnesium / Q000097:blood / Q000494:pharmacology*; D008278:Magnesium Sulfate / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D015233:Models, Statistical; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000293:injuries* / Q000473:pathology; D054792:Muscle, Striated / Q000473:pathology; D020556:Neutrophil Infiltration; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*
D016209:Interleukin-8; D008315:Malondialdehyde; D008278:Magnesium Sulfate; D008274:Magnesium