title: Rationale for reading fluconazole MICs at 24 hours rather than 48 hours when testing Candida spp. by the CLSI M27-A2 standard method.
abstract: We investigated if CLSI M27-A2 Candida species breakpoints for fluconazole MIC are valid when read at 24 h. Analysis of a data set showed good correlation between 48- and 24-h MICs, as well as similar outcomes and pharmacodynamic efficacy parameters, except for isolates in the susceptible dose-dependent category, such as Candida glabrata.
authors.affiliation: University of Texas Medical School at Houston, Texas 77030, USA. Luis.Ostrosky-Zeichner@uth.tmc.edu
authors.forename: Luis
authors.initials: L
authors.lastname: Ostrosky-Zeichner
authors.forename: John H
authors.initials: JH
authors.lastname: Rex
authors.forename: Michael A
authors.initials: MA
authors.lastname: Pfaller
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Diekema
authors.forename: Barbara D
authors.initials: BD
authors.lastname: Alexander
authors.forename: David
authors.initials: D
authors.lastname: Andes
authors.forename: Steven D
authors.initials: SD
authors.lastname: Brown
authors.forename: Vishnu
authors.initials: V
authors.lastname: Chaturvedi
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Ghannoum
authors.forename: Cindy C
authors.initials: CC
authors.lastname: Knapp
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Sheehan
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walsh
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology; D016208:Databases, Factual; D015725:Fluconazole / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards* / Q000706:statistics & numerical data; D008954:Models, Biological; D013997:Time Factors
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole