title: Inhibition of microsomal triglyceride transfer protein expression and atherogenic risk factor apolipoprotein B100 secretion by tanshinone IIA in HepG2 cells.
abstract: Salvia miltiorrhiza Bunge is known to be effective for the treatment of cardiovascular diseases. Here, we have isolated tanshinone IIA (T-IIA) from S. miltiorrhiza Bunge. The aim of this study is to address the mechanisms where apolipoprotein B-100 (ApoB) regulation is associated with T-IIA, since T-IIA regulates the lipoprotein metabolism in liver cells. Human HepG2 cells treated with T-IIA for 24 h exerted a dose-dependent inhibitory effect on ApoB secretion together with triglyceride. However, another secretory protein, albumin, was unaffected by T-IIA treatment, indicating that the effect of T-IIA is specific for ApoB secretion. T-IIA decreased the transcription level of microsomal triglyceride transfer protein gene, suggesting that lipoprotein assembly is likely to be involved in the inhibited ApoB secretion. Interestingly, T-IIA inhibited ApoB secretion via a proteasome-dependent pathway. Our results suggest that T-IIA is an influential inhibitor of ApoB secretion and triglyceride secretion in liver cells.
authors.affiliation: Department of Biological Science, Sungkyunkwan University, Jangan-Gu, Suwon City, Kyunggi-Do 440-746, Korea.
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Kang
authors.forename: Un-Ho
authors.initials: UH
authors.lastname: Jin
authors.forename: Hyen-Wook
authors.initials: HW
authors.lastname: Chang
authors.forename: Jong-Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Seung Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Son
authors.forename: Young-Chae
authors.initials: YC
authors.lastname: Chang
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
authors.forename: Cheorl-Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D045784:Abietanes; D000418:Albumins / Q000378:metabolism; D053299:Apolipoprotein B-100 / Q000187:drug effects* / Q000378:metabolism; D002352:Carrier Proteins / Q000187:drug effects* / Q000378:metabolism; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D010616:Phenanthrenes / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism; D013379:Substrate Specificity; D014158:Transcription, Genetic; D014280:Triglycerides / Q000378:metabolism
chemical_list: D045784:Abietanes; D000418:Albumins; D053299:Apolipoprotein B-100; D002352:Carrier Proteins; D010616:Phenanthrenes; C497387:SREBF1 protein, human; D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides; C106092:microsomal triglyceride transfer protein; C021751:tanshinone; D046988:Proteasome Endopeptidase Complex