title: Comparative evaluation of enzyme-linked immunosorbent assay based on crude and purified antigen in the diagnosis of canine visceral leishmaniasis in symptomatic and oligosymptomatic dogs.
abstract: This study evaluated the performance of crude total antigen (CTA) and fucose-mannose ligand antigen (FML) in an enzyme-linked immunosorbent assay for diagnosis of canine visceral leishmaniasis (CVL). The assays used sera from known negative controls (n=30), clinically symptomatic (n=30) and oligosymptomatic (n=30) parasitologically proven infection (by microscopy). Aspirates of popliteal lymph node from infected canines were colleted to score parasitism and compared with the ELISA results. The study indicated that FML used in ELISA provided high sensitivity for detecting oligosymptomatic dogs (90%) and CTA showed greater sensitivity than FML for symptomatic canines (90%). In oligosymptomatic dogs, specificity was 100% for CTA-ELISA, but in symptomatic dogs, FML specificity was higher (96.7%) than CTA-ELISA (93.3%). A significant correlation was observed between the degree of parasitism and the results obtained in CTA-ELISA. Since no available antigen offers 100% specificity and sensitivity for CVL diagnosis, the choice of antigen used must depend on the aim of the investigation.
authors.affiliation: Departamento de Clinica, Cirurgia e Reprodução Animal, Medicina Veterinária, Universidade Estadual Paulista, Araçatuba, SP, Brazil. vmflima@fmva.unesp.br
authors.forename: Teresinha Cristina
authors.initials: TC
authors.lastname: Cândido
authors.forename: Sílvia Helena Venturoli
authors.initials: SH
authors.lastname: Perri
authors.forename: Tatiana de Oliveira
authors.initials: Tde O
authors.lastname: Gerzoschkwitz
authors.forename: Maria Cecília Rui
authors.initials: MC
authors.lastname: Luvizotto
authors.forename: Valéria Marçal Felix
authors.initials: VM
authors.lastname: de Lima
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000662:veterinary*; D008198:Lymph Nodes / Q000469:parasitology; D012680:Sensitivity and Specificity
chemical_list: D000953:Antigens, Protozoan