title: Efficacy and safety of blue-light scleral cross-linking.
abstract: OBJECTIVE
  To evaluate the efficacy of blue-light scleral cross-linking as well as its safety in preventing retinal damage beneath the treated sclera.
  
  
  METHODS
  Six rabbits were unilaterally treated with topical riboflavin (0.5%) and blue light (465 nm) on the equatorial sclera using a light emitting diode source with an exposure area of 9 mm in diameter. Four weeks after the treatment, the animals were euthanized and the exposed sclera and contralateral eye sclera excised for comparative testing of biomechanical rigidity and histologic retinal cellular damage. Extensiometry was performed to evaluate the stress-strain curve of treated versus untreated sclera, and light microscopy of the treated sclera and underlying retina were also comparatively evaluated.
  
  
  RESULTS
  Blue-light scleral cross-linking showed a three-fold increased stiffening in all tested animals in the stress-strain curve. Histological investigation revealed no retinal damage in any of the treated eyes.
  
  
  CONCLUSIONS
  Scleral cross-linking with riboflavin and blue light (465 nm) has a stiffening effect on the sclera without histological tissue damage to the retina.
authors.affiliation: IROC - Institute of Refractive and Ophthalmic Surgery, Zurich, Switzerland. info@iroc.ch
authors.forename: Hans Peter
authors.initials: HP
authors.lastname: Iseli
authors.forename: Eberhard
authors.initials: E
authors.lastname: Spoerl
authors.forename: Peter
authors.initials: P
authors.lastname: Wiedemann
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Krueger
authors.forename: Theo
authors.initials: T
authors.lastname: Seiler
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D004548:Elasticity; D008027:Light; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use* / Q000633:toxicity; D011817:Rabbits; D012160:Retina / Q000187:drug effects / Q000473:pathology; D012256:Riboflavin / Q000627:therapeutic use* / Q000633:toxicity; D012590:Sclera / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D013552:Swine
chemical_list: D017319:Photosensitizing Agents; D003094:Collagen; D012256:Riboflavin