Comparison of the immunomodulatory effects of L. donovani and L. major excreted-secreted antigens, particulate and soluble extracts and viable parasites on human dendritic cells.
In an experimental model of human monocyte-derived dendritic cells (DCs), the immunophenotype of mature DCs infected with Leishmania donovani and Leishmania major showed a weak decrease in the cell surface expression of CD40, CD86, HLA-DR and DC-SIGN compared with uninfected control DCs. This immunomodulatory effect was more pronounced after stimulation with excreted-secreted antigens (ESA) of both species but absent after stimulation with particulate and soluble extracts. Infection with viable promastigotes, as well as stimulation with ESA from L. donovani and L. major, decreased IL-10 and IL-12p70 secretion. To our knowledge, this is the first direct demonstration that ESA from Leishmania promastigotes can stimulate DCs in the same manner as viable promastigotes.
Revest
Matthieu
M
Unité INSERM 522, Centre Hospitalier Universitaire Pontchaillou, Rennes, France.
Donaghy
Ludovic
L
Cabillic
Florian
F
Guiguen
Claude
C
Gangneux
Jean-Pierre
JP
D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000276:immunology*; D000954:Antigens, Surface / Q000096:biosynthesis / Q000276:immunology; D019013:CD40 Antigens / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology* / Q000469:parasitology*; D015536:Down-Regulation / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D007893:Leishmania donovani / Q000254:growth & development / Q000276:immunology*; D018320:Leishmania major / Q000254:growth & development / Q000276:immunology*
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D019013:CD40 Antigens; D016207:Cytokines