title: The WAVE2 complex regulates T cell receptor signaling to integrins via Abl- and CrkL-C3G-mediated activation of Rap1.
abstract: WAVE2 regulates T cell receptor (TCR)-stimulated actin cytoskeletal dynamics leading to both integrin clustering and affinity maturation. Although WAVE2 mediates integrin affinity maturation by recruiting vinculin and talin to the immunological synapse in an Arp2/3-dependent manner, the mechanism by which it regulates integrin clustering is unclear. We show that the Abl tyrosine kinase associates with the WAVE2 complex and TCR ligation induces WAVE2-dependent membrane recruitment of Abl. Furthermore, we show that WAVE2 regulates TCR-mediated activation of the integrin regulatory guanosine triphosphatase Rap1 via the recruitment and activation of the CrkL-C3G exchange complex. Moreover, we demonstrate that although Abl does not regulate the recruitment of CrkL-C3G into the membrane, it does affect the tyrosine phosphorylation of C3G, which is required for its guanine nucleotide exchange factor activity toward Rap1. This signaling node regulates not only TCR-stimulated integrin clustering but also affinity maturation. These findings identify a previously unknown mechanism by which the WAVE2 complex regulates TCR signaling to Rap1 and integrin activation.
authors.affiliation: Department of Immunology, College of Medicine, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Nolz
authors.forename: Lucas P
authors.initials: LP
authors.lastname: Nacusi
authors.forename: Colin M
authors.initials: CM
authors.lastname: Segovis
authors.forename: Ricardo B
authors.initials: RB
authors.lastname: Medeiros
authors.forename: Jason S
authors.initials: JS
authors.lastname: Mitchell
authors.forename: Yoji
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Daniel D
authors.initials: DD
authors.lastname: Billadeau
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D004789:Enzyme Activation; D005353:Fibronectins / Q000378:metabolism; D020735:Guanine Nucleotide-Releasing Factor 2 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D019169:Jurkat Cells; D046912:Multiprotein Complexes / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D016315:Proto-Oncogene Proteins c-abl / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000235:genetics / Q000378:metabolism*; D020827:rap1 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C082869:CRKL protein; D005353:Fibronectins; D020735:Guanine Nucleotide-Releasing Factor 2; D016023:Integrins; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D011948:Receptors, Antigen, T-Cell; D051300:Wiskott-Aldrich Syndrome Protein Family; D018799:Intercellular Adhesion Molecule-1; D016315:Proto-Oncogene Proteins c-abl; D020827:rap1 GTP-Binding Proteins