title: [Optimization of the supercritical fluid extraction process of Tuirezhitongsan].
abstract: OBJECTIVE
  To optimize the extraction process of Tuirezhitongsan and extract the pharmacodynamically active fractions.
  
  
  METHODS
  Orthogonal test methods and supercritical-CO(2) fluid extraction (SFE-CO(2)) were used for the extraction, and the the pharmacodynamically active fractions were screened with analgesic experiments.
  
  
  RESULTS
  The optimal extraction of the active fractions was achieved with the extraction pressure at 25 MPa, extraction temperature at 45 degrees celsius, resolution pressure at 6.7 MPa, and resolution temperature at 50 degrees celsius.
  
  
  CONCLUSIONS
  SFE- CO(2) is rapid, efficient, and energy-saving in the extraction of the active fractions in Tuirezhotongsan, and provides a mew method for extraction of traditional Chinese medicine.
authors.affiliation: Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou, China. zhoubj163@163.com
authors.forename: Ben-jie
authors.initials: BJ
authors.lastname: Zhou
authors.forename: Qing-wei
authors.initials: QW
authors.lastname: Huang
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Feng
mesh_terms: D019342:Acetic Acid; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005260:Female; D008297:Male; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; C539568:tuirezhitongsan; D019342:Acetic Acid