Production of fructosyltransferase by Aureobasidium sp. ATCC 20524 in batch and two-step batch cultures.
A comparison of fructosyltransferase (EC 2.4.1.9) production by Aureobasidium sp. ATCC 20524 in batch and two step batch cultures was investigated in a 1-l stirred tank reactor using a sucrose supply of 200 g/l. Results showed that the innovative cultivation in two step of Aureobasidium sp. produced more fructosyltransferase (FFase) than the single batch culture at the same sucrose concentration with a maximal enzyme production of 523 U/ml, which was 80.5% higher than the one obtained in the batch culture. The production of fructooligosaccharides (FOSs) was also analyzed; their concentration reached a maximum value of 160 g/l the first day in the two-step culture and 127 g/l in the single-batch mode. The use of the two-step batch culture with Aureobasidium sp. ATCC 20524 in allowing the microorganism to grow up prior to the induction of sucrose (second step), proved to be a powerful method for producing fructosyltransferase and FOSs.
Salinas
Martín A
MA
PROIMI-CONICET (Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Tecnológicas), Av. Belgrano y Pje. Caseros, San Miguel de Tucumán, CP, 4000, Argentina. msalinas@proimi.org.ar
Perotti
Nora I
NI
D001203:Ascomycota / Q000737:chemistry / Q000201:enzymology*; D019149:Bioreactors / Q000382:microbiology*; D046508:Culture Techniques / Q000379:methods*; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D006602:Hexosyltransferases / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D001426:Bacterial Proteins
D005656:Fungal Proteins; D006602:Hexosyltransferases; C021451:inulosucrase; D001426:Bacterial Proteins