Application of liquid-based preparation to fine needle aspiration cytology in breast cancer.
OBJECTIVE
To examine the performance of liquid-based cytology (LBC) in breast cytology to confirm the diagnosis of carcinoma.


METHODS
Using cell clusters directly scratched from surgically removed tumor masses, we examined the immunocytochemistry, molecular biology and cytomorphology of the specimens.


RESULTS
LBC was very useful for gene analysis and evaluating the immunocytochemistry. The cytologic features of LBC were slightly different from those ofa conventional aspiration cytology smear.


CONCLUSIONS
LBC is a promising method for improving the standardization ofpreparations in breast cytology, although care should be taken to account for its characteristic cytologic features. The quantitative analysis of HER-2 mRNA correlated with the results of immunohistochemistry.
Komatsu
Kyoko
K
Pathology Laboratory, Nihon University Itabashi Hospital, Tokyo, Japan.
Nakanishi
Yoko
Y
Seki
Toshimi
T
Yoshino
Atsuko
A
Fuchinoue
Fumi
F
Amano
Sadao
S
Komatsu
Akio
A
Sugitani
Masahiko
M
Nemoto
Norimichi
N
D002288:Adenocarcinoma, Mucinous / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D044963:Biopsy, Fine-Needle / Q000379:methods; D001943:Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D003581:Cytodiagnosis / Q000379:methods; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human