[Effect of porcine relaxin on NO production of human microvascular endothelial cells].
OBJECTIVE
To observe effect of porcine relaxin(pRLX) on NO production of human microvascular endothelial cells(HMVECs) and discuss its possible mechanism.


METHODS
iNOS and cNOS expression of HMVECs with or without pRLX were detected using western blotting. NO production of HMVECs with pRLX at different concentration or different time were determined by method of Griess. NO production of pRLX of HMVECs plus Non-selective NOS inhibitor NG-monomethyl-L-arginine(L-NMMA), selective iNOS inhibitor aminoguanidine(AG) or nuclear factors-kappaB (NF-kappaB) inhibitor pyrrolidine dithiocarbamate(PDTC) were also analysed.


RESULTS
pRLX promoted iNOS protein expression of HMVECs, but not cNOS protein expression. NO production of HMVECs was promoted by pRLX on concentration-dependent pattern instead of time-dependent one. AG, L-NMMA and PDTC were showed to block the effect of pRLX on NO production of HMVECs.


CONCLUSIONS
pRLX promote iNOS expression and NO production of HMVECs.
Fu
Rui-bin
RB
Department of Cardiology, Liuhuaqiao Hospital, Guangzhou, China. furuibin@21cn.com
Qiu
Jian
J
Xiang
Ding-cheng
DC
Ma
Jun
J
He
Jian-xin
JX
Hong
Chang-jiang
CJ
Zhang
Jin-xia
JX
D000818:Animals; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000098:blood supply; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D012065:Relaxin / Q000494:pharmacology*; D013552:Swine; D013997:Time Factors
D009569:Nitric Oxide; D012065:Relaxin; D052247:Nitric Oxide Synthase Type II