title: [Effects of minimal persistent inflammation on nasal mucosa of experimental allergic rhinitis in guinea pigs].
abstract: OBJECTIVE
  To establish an animal model of minimal persistent inflammation (MPI) of allergic rhinitis in guinea pigs and to investigate the changes of nasal mucosa. The expression of transforming growth factor-beta1 (TGF-beta1) and matrix metalloproteinases-9 (MMP-9) were discussed.
  
  
  METHODS
  Thirty male Hartley guinea pigs were randomly divided into two groups: MPI model group and control group randomly, with fifteen animals in each group. Guinea pigs from MPI model group were sensitized intraperitoneally by injection of suspension of ovalbumin (OVA) and aluminum hydroxide in 0.9% physiological saline. Then, repeated local booster sensitization with low concentration of OVA suspension into the nasal cavity was performed to establish MPI models. Alcian blue-periodic acid-Schiff (AB-PAS) staining and Masson's trichrome (MT) staining were used to determine the number of goblet cells and collagen deposition within the basement membrane of epithelium. The expression and distribution of TGF-beta1 and MMP-9 in nasal mucosa were estimated by double immunofluorescence under a confocal laser scan microscopy system.
  
  
  RESULTS
  Compared with the control group, the increased goblet cells (t = 13.720, P < 0.05) in nasal epithelium together with the increased collagen fibrils (t = 4.542, P <0.05) within the basement membrane of epithelium were observed in the MPI model group. There was nearly no expression of TGF-beta1, in the control group and the expression of MMP-9 was only found in the epithelium cell. In contrast, there was significantly higher expression of TGF-beta1 and MMP-9 (t = 25.218, P <0.05) in nasal mucosa of MPI model group than that in control group. TGF-beta1 mainly expressed in the epithelium cell, the infiltrated inflammatory cell and extracellular matrix, while MMP-9 expressed in the epithelium cell and the infiltrated inflammatory cell.
  
  
  CONCLUSIONS
  Long time MPI in allergic rhinitis resulted in some changes of tissue remodeling in nasal mucosa. TGF-beta1 and MMP-9 may play an important role in disease progression.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Lei
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Cui
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Jiang
authors.forename: Hong
authors.initials: H
authors.lastname: Kong
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Zhu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Dong
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007249:Inflammation; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009297:Nasal Mucosa / Q000378:metabolism*; D006255:Rhinitis, Allergic, Seasonal / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D053773:Transforming Growth Factor beta1; D020780:Matrix Metalloproteinase 9