Differing expression of genes involved in non-transferrin iron transport across plasma membrane in various cell types under iron deficiency and excess.
We studied the effect of iron deficiency, i.e., 24-h preincubation in iron-free medium, and the effect of high level of non-transferrin iron, i.e., the preincubation in ferric citrate medium containing 500 microM ferric citrate, on the expression of DMT1, Dcytb, ferroportin, hephaestin, and ceruloplasmin in various functional types of human cells. The expression of these proteins potentially involved in non-transferrin iron transport across cell membranes was tested on mRNA level by quantitative real-time PCR as well as on protein level by western blot analysis in Caco-2 (colorectal carcinoma), K562 (erythroleukemia), and HEP-G2 (hepatocellular carcinoma) cells. We found that changes in non-transferrin iron availability, i.e., iron deficiency and high level of non-transferrin iron, affect the expression of tested proteins in a cell type-specific manner. We also demonstrated that changes in the expression on mRNA level do not often correlate with relevant changes on protein level.
Balusikova
Kamila
K
Department of Cell and Molecular Biology & Center for Research of Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
Neubauerova
Jitka
J
Dostalikova-Cimburova
Marketa
M
Horak
Jiri
J
Kovar
Jan
J
D000818:Animals; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002570:Ceruloplasmin / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D003573:Cytochrome b Group / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism; D000098742:Ferroportin; D000099236:Solute Carrier Family 11, Member 2
D027682:Cation Transport Proteins; D002570:Ceruloplasmin; D003470:Culture Media; D003573:Cytochrome b Group; D007501:Iron; D008565:Membrane Proteins; D010088:Oxidoreductases; D014168:Transferrin; D000098742:Ferroportin; D000099236:Solute Carrier Family 11, Member 2; C492164:HEPH protein, human; C422926:CYBRD1 protein, human