Treatment and control of mycoplasma contamination in Plasmodium falciparum culture.
A comparative efficacy of four antibiotics, plasmocin (macrolid), Biomyc-1, -2, (tetracycline), and Biomyc-3, and Mycoplasma Removing Agent (quinolone derivatives) was determined for elimination of mycoplasma from Plasmodium falciparum culture. Presence of mycoplasma was detected using enzyme-PCR-based mycoplasma detection kit and survival of malaria parasite was determined in Giemsa's stained smear made from treated and untreated cultures. It was observed that a combination of Biomyc-1 and -2 killed malaria parasites within 24 h, whereas plasmocin and Biomyc-3 caused slow death of malaria parasite stretched over a period of 6 days. The only compound which did not kill malaria parasite and eradicated mycoplasma from P. falciparum culture was observed to be MRA.
Singh
Shubhra
S
Division of Parasitology, Central Drug Research Institute, Lucknow, India.
Puri
S K
SK
Srivastava
Kumkum
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004866:Equipment Contamination; D006863:Hydrogen-Ion Concentration; D018942:Macrolides / Q000494:pharmacology; D009174:Mycoplasma / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D010274:Parasitology / Q000379:methods; D010963:Plasmodium falciparum / Q000187:drug effects / Q000254:growth & development / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D015363:Quinolones / Q000494:pharmacology; D012692:Serial Passage; D013752:Tetracycline / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D003470:Culture Media; D018942:Macrolides; D015363:Quinolones; D013752:Tetracycline