title: Role of Ebola virus VP30 in transcription reinitiation.
abstract: VP30 is a phosphoprotein essential for the initiation of Ebola virus transcription. In this work, we have studied the effect of mutations in VP30 phosphorylation sites on the ebolavirus replication cycle by using a reverse genetics system. We demonstrate that VP30 is involved in reinitiation of gene transcription and that this activity is affected by mutations at the phosphorylation sites.
authors.affiliation: INSERM, U758, Filovirus Laboratory, 21 Av. Tony Garnier, 69365 Lyon, Cedex 07, France.
authors.forename: Miguel J
authors.initials: MJ
authors.lastname: Martínez
authors.forename: Nadine
authors.initials: N
authors.lastname: Biedenkopf
authors.forename: Valentina
authors.initials: V
authors.lastname: Volchkova
authors.forename: Bettina
authors.initials: B
authors.lastname: Hartlieb
authors.forename: Nathalie
authors.initials: N
authors.lastname: Alazard-Dany
authors.forename: Olivier
authors.initials: O
authors.lastname: Reynard
authors.forename: Stephan
authors.initials: S
authors.lastname: Becker
authors.forename: Viktor
authors.initials: V
authors.lastname: Volchkov
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D029043:Ebolavirus / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D014157:Transcription Factors; C479897:VP30 protein, ebola virus; D014764:Viral Proteins