Multi-residue analysis of steroids at sub-ng/L levels in surface and ground-waters using liquid chromatography coupled to tandem mass spectrometry.
Most analytical methodologies currently available for the determination of steroids in water only identify a few representative compounds (mainly estrogens). In this context, a multi-residue methodology based on liquid chromatography-tandem mass spectrometry was developed for the determination of 26 steroids including natural and synthetic estrogens, progestagens and androgens. The method described involves limited sample preparation as it includes a filtration followed by a single solid-phase extraction step using a C18 cartridge. The analytical procedure allows the determination of the target analytes in the lower ng/L range, with recoveries above 80%. The methodology was successfully applied to the analysis of steroids in several surface and ground-waters. In all the waters, estrogens, androgens and/or progestagens were determined.
Vulliet
Emmanuelle
E
Service Central d'Analyse du CNRS-USR59, Chemin du Canal, B.P. 22, F-69360 Solaize, France. e.vulliet@sca.cnrs.fr
Wiest
Laure
L
Baudot
Robert
R
Grenier-Loustalot
Marie-Florence
MF
D002853:Chromatography, Liquid / Q000379:methods*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D013256:Steroids / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry*
D013256:Steroids; D014874:Water Pollutants, Chemical