title: Effect of iron-quercetin complex on reduction of nitrite in in vitro and in vivo systems.
abstract: This study investigated whether reducing agents such as quercetin and iron(II) facilitate formation of nitric oxide (NO) gas from orally ingested nitrite in an vivo study. When 3 mg/kg Na (15)NO2 was orally administered to rats with or without iron(II) or quercetin, Hb (15)NO, which is indicative of systemic (15)NO, was detected in the blood, with the maximum blood concentration of Hb (15)NO at 15 min after nitrite or nitrite plus quercetin treatment, whereas after administration of nitrite plus iron(II) or nitrite plus iron(II) and quercetin, the time was shortened to 10 min. Interestingly, iron(II), quercetin, or iron(II) plus quercetin did not affect the total amount of Hb (15)NO generated from orally administered Na (15)NO2. However, the systemic nitrite concentration was significantly decreased in the presence of iron(II) or iron(II) plus quercetin. These results may indicate that iron(II) is critical to the generation of NO gas from nitrite, whereas quercetin contributed little under the in vivo experimental conditions.
authors.affiliation: Department of Pharmacology, The University of Tokushima Graduate School, 1-78-1 Sho-machi, Tokushima 770-8505, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Iwanaga
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawazoe
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ishizawa
authors.forename: Sakiko
authors.initials: S
authors.lastname: Orino
authors.forename: Shuhei
authors.initials: S
authors.lastname: Tomita
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Kanematsu
authors.forename: Nagakatsu
authors.initials: N
authors.lastname: Harada
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Mori
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tsuchihashi
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tamaki
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009566:Nitrates / Q000737:chemistry / Q000378:metabolism; D009569:Nitric Oxide / Q000737:chemistry / Q000378:metabolism; D009573:Nitrites / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011794:Quercetin / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; D011794:Quercetin; D007501:Iron