Liquid chromatographic analysis of guanidino compounds using furoin as a new fluorogenic reagent.
Furoin, a benzoin analogue, was examined as novel fluorogenic reagent for the selective and sensitive LC determination of various guanidines after pre-column derivatization. The derivatization reaction was carried out at 100 degrees C for 5 min to give adducts that were separated on a Phenomenex Synergi MAX-RP column and detected at lambda(em)=410 nm with lambda(ex)=325 nm. The reagent showed to be useful both for determining together arginine (Arg) and creatine (CT) in dietary supplements under elution isocratic conditions and for the simultaneous analysis of a variety of guanidines in biological samples (human plasma and urine) under elution gradient conditions. The detection limits ranged from 7 to 25 fmol. Recovery studies showed good results for all determined guanidino compounds (85.6-106.2%; R.S.D.=1.1-6.2%).
Gatti
R
R
Dipartimento di Scienze Farmaceutiche, Alma Mater Studiorum-Università di Bologna, Via Belmeloro 6, 40126 Bologna, Italy. rita.gatti2@unibo.it
Gioia
M G
MG
D001573:Benzoin / Q000031:analogs & derivatives* / Q000737:chemistry*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis; D005663:Furans; D006146:Guanidines / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D015394:Molecular Structure; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013997:Time Factors
C515387:3,3'-furoin; D005663:Furans; D006146:Guanidines; D007202:Indicators and Reagents; D001573:Benzoin