Efficacy of common laboratory disinfectants and heat on killing trypanosomatid parasites.
The disinfectants TriGene, bleach, ethanol and liquid hand soap, and water and temperature were tested for their ability to kill bloodstream forms of Trypanosoma brucei, epimastigotes of Trypanosoma rangeli and promastigotes of Leishmania major. A 5-min exposure to 0.2% TriGene, 0.1% liquid hand soap and 0.05% bleach (0.05% NaOCl) killed all three trypanosomatids. Ethanol and water destroyed the parasites within 5 min at concentrations of 15-17.5% and 80-90%, respectively. All three organisms were also killed when treated for 5 min at 50 degrees C. The results indicate that the disinfectants, water and temperature treatment (i.e. autoclaving) are suitable laboratory hygiene measures against trypanosomatid parasites.
Wang
Xia
X
BioMedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, NR4 7TJ, UK. dsteverding@hotmail.com.
Jobe
Momodou
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Tyler
Kevin M
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Steverding
Dietmar
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