Novel caspase-suicide proteins for tamoxifen-inducible apoptosis.
Taking advantage of a mutant estrogen receptor ligand binding domain (ER(T2)), we developed novel Caspase fusion proteins for inducible apoptosis. We show that Caspase-ER(T2) fusion proteins become specifically activated by the synthetic ligand 4-OH- tamoxifen and rapidly induce apoptotic cell death in human, murine, and zebrafish cells. This novel tool for targeted cell ablation greatly facilitates the generation of disease models as well as developmental and regeneration studies in model organisms.
Chu
Yuanyuan
Y
Helmholtz Zentrum München, German Research Center for Environmental Health, Institute for Developmental Genetics, 85764 Munich/Neuherberg, Germany.
Senghaas
Niklas
N
Köster
Reinhard W
RW
Wurst
Wolfgang
W
Kühn
Ralf
R
D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001665:Binding Sites / Q000187:drug effects / Q000235:genetics; D020169:Caspases / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002461:Cell Line, Transformed; D041722:Genes, Transgenic, Suicide / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D020845:Selective Estrogen Receptor Modulators / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology*; D015027:Zebrafish
D018931:Antineoplastic Agents, Hormonal; D020845:Selective Estrogen Receptor Modulators; D013629:Tamoxifen; D020169:Caspases