title: Validation of HPLC method for quantitative determination of Tinosorb S and three other sunscreens in a high protection cosmetic product.
abstract: A chromatographic method (high performance liquid chromatography) with a diode array detector was developed for simultaneous assay of Tinosorb S (bis-ethylhexyloxyphenol methoxyphenyl triazine) with three other sunscreen agents [benzophenone-3, butyl methoxydibenzoylmethane (avobenzone) and ethylhexyl methoxycinnamate] in high protection sunscreen. Separations were performed on a RP-18 Nucleodur Gravity column (150 x 4.6 mm, 5 mum) eluted with a ternary gradient mixture constituted of tetrahydrofuran, acetonitrile and an aqueous solution of acetic acid. The quantitative analysis was achieved with internal calibration performed with octyl dimethyl para-aminobenzoate (PABA) at 330 nm. In accordance with the analytical references (SFSTP, ICH, ISO...), the accuracy of the method was evaluated using a statistical approach of the validation parameters (specificity, response function, linearity, precision and trueness). For each studied ultraviolet filter, an accuracy profile was determined on a predicted range. These profiles show a graphical representation of the recovery percentage and confidence limits centred on 100%. The method is validated and can be used for analysis in cosmetic sunscreen products.
authors.affiliation: Laboratoires ASEPTA, Recherche et Développement Analytique, 1-3, avenue Prince Albert II, B.P. 649, MC 98013, Monaco Cedex, France. ldencausse@asepta.mc
authors.forename: L
authors.initials: L
authors.lastname: Dencausse
authors.forename: A
authors.initials: A
authors.lastname: Galland
authors.forename: J L
authors.initials: JL
authors.lastname: Clamou
authors.forename: J
authors.initials: J
authors.lastname: Basso
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003358:Cosmetics / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013473:Sunscreening Agents / Q000032:analysis*; D014227:Triazines / Q000032:analysis*
chemical_list: D003358:Cosmetics; D010636:Phenols; D013473:Sunscreening Agents; D014227:Triazines; C439083:bis-ethylhexyloxyphenol methoxyphenyl triazine