Mouse adrenal chromaffin cell isolation.
Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.
Kolski-Andreaco
Aaron
A
Department of Physiology and Biophysics, University of California, Irvine, CA, USA. aaron.kolski-andreaco@jove.com
Cai
Haijiang
H
Currle
D Spencer
DS
Chandy
K George
KG
Chow
Robert H
RH
D000311:Adrenal Glands / Q000166:cytology*; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D019439:Chromaffin Cells; D004210:Dissection; D051379:Mice