title: [Effects of trichostatin A on the interaction between HBx and histone deacetylase protein 1].
abstract: OBJECTIVE
  To study the effects of trichostatin A (TSA) on protein-protein interaction between HBx and histone deacetylase protein 1 (HDAC1).
  
  
  METHODS
  Both HBx and HDAC1 expressing vectors were constructed by the method of routine molecular cloning. The expression of HBx and HDAC1 were observed by Western blot assay. The protein-protein interaction was tested between HBx and HDAC1 by GST pull-down in vitro as well as co-immunoprecipitation in vivo.
  
  
  RESULTS
  Both HBx and HDAC1 expressing vectors were successfully constructed. Protein-protein interaction between HBx and HDAC1 existed both in vitro and in vivo. TSA, an inhibitor of HDAC1, had no effect on the interaction between HBx and HDAC1.
  
  
  CONCLUSIONS
  HBx interacts with HDAC1 in vivo and in vitro in a non- TSA dependent way.
authors.affiliation: Institute of Hepatology, Beijing Military General Hospital, Beijing 100700, China.
authors.forename: Ju-qiang
authors.initials: JQ
authors.lastname: Han
authors.forename: Qi-nong
authors.initials: QN
authors.lastname: Ye
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Ding
authors.forename: Jie-zhi
authors.initials: JZ
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Cui-fen
authors.initials: CF
authors.lastname: Huang
mesh_terms: D056284:Histone Deacetylase 1 / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000378:metabolism*; D047468:Immunoprecipitation; D010957:Plasmids; D025941:Protein Interaction Mapping; D015534:Trans-Activators / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D006877:Hydroxamic Acids; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; C012589:trichostatin A; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1