Alterations of peripheral blood CD5+ B cells in inflammatory bowel disease.
OBJECTIVE
CD5(+) B cells comprise a unique subset of B cells that modulates innate as well as autoimmune systems. The aim of this study was to investigate alterations of the circulating CD5(+) B-cell subset in patients with inflammatory bowel disease (IBD) by evaluating various clinical parameters, including therapeutic regimens.


METHODS
Thirty-four patients with ulcerative colitis (UC), 19 patients with Crohn's disease (CD), and 46 healthy control subjects were enrolled in this study. CD5(+) B cells in peripheral blood collected from each subject were analyzed by flow cytometry. Multiple regression analysis was carried out to evaluate the factors related to the circulating CD5(+) B-cell subset in the IBD patients. In an in vitro examination, dexamethasone-induced apoptosis in peripheral blood B cells was examined by detecting cell surface binding of the annexin-V antibody.


RESULTS
Age and gender in the control subjects did not influence the circulating CD5(+) B-cell subset. Multiple regression analysis showed that the presence of UC, corticosteroid therapy, and number of white blood cells in peripheral blood each had a significant influence in decreasing the number of circulating CD5(+) B cells in the IBD patients. Furthermore, in vitro results showed that dexamethasone treatment significantly induced apoptosis in CD5(+) B cells, though apoptosis was similarly observed in CD5(-) B cells.


CONCLUSIONS
CD5(+) B cells may be involved in the pathogenesis of UC, and modulation of this subset by corticosteroid therapy may play a role in the treatment of IBD patients.
Mishima
Yoshiyuki
Y
Department of Internal Medicine II, Shimane University School of Medicine, Japan.
Ishihara
Shunji
S
Amano
Yuji
Y
Oshima
Naoki
N
Kadota
Chikara
C
Otani
Aya
A
Moriyama
Ichiro
I
Li
Yong-Yu
YY
Aziz
M M
MM
Kinoshita
Yoshikazu
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D016175:B-Lymphocyte Subsets / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism*; D018956:CD5 Antigens / Q000097:blood* / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood* / Q000188:drug therapy / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D018956:CD5 Antigens; D005938:Glucocorticoids