title: Apoptosis and anti-apoptotic heat shock proteins in canine cutaneous infundibular keratinizing acanthomas and squamous cell carcinomas.
abstract: Cell stress and death are linked in the neoplastic process, and heat shock proteins appear to play an important role by inhibiting apoptotic pathways. The apoptotic rates in 9 canine infundibular keratinizing acanthomas (IKAs) and 17 canine squamous cell carcinomas (SCCs) were correlated with the immunohistochemical expression of caspase-3 and the antiapoptotic heat shock proteins Hsp27, 72 and 73. Apoptosis was evaluated using the terminal deoxynucleotidyl transferase biotin-dUTP nick end labelling (TUNEL) method. The absence of a correlation between the TUNEL index and active-caspase-3 expression, a paucity of active-caspase-3-positive cells and Hsp72 over-expression were considered to be indicative of inhibition of apoptosis, and suggestive that inhibition of cell death plays a key role in oncogenesis and tumour growth of some canine skin neoplasms.
authors.affiliation: Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro, 45-64100 Teramo, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Bongiovanni
authors.forename: Mariarita
authors.initials: M
authors.lastname: Romanucci
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Fant
authors.forename: Marie
authors.initials: M
authors.lastname: Lagadic
authors.forename: Leonardo
authors.initials: L
authors.lastname: Della Salda
mesh_terms: D049309:Acanthoma / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary; D020287:In Situ Nick-End Labeling
chemical_list: D006360:Heat-Shock Proteins