Dietary trans-resveratrol bioavailability and effect on CCl4-induced liver lipid peroxidation.
OBJECTIVE
Several in vitro studies have demonstrated the ability of pure trans-resveratrol (t-Res) to act as an anti-oxidant, but the scientific literature is lacking in in vivo studies dealing with dietary t-Res bioavailability in oxidative stress models. Our aim was to investigate the bioavailability of t-Res from dietary sources and its effect on an animal model of carbon tetrachloride (CCl4)-induced liver lipid peroxidation.


METHODS
Ten rats were intragastrically administered for 14 days with a grape-stalk extract determining a daily t-Res dosage of 3 mg/kg. The control group (10 rats) was daily injected with the vehicle solvent without the t-Res extract. After 1 week, the induction of liver lipid peroxidation by CCl4 injection was carried out. Serum and liver samples, at different time intervals, were collected to evaluate t-Res content, by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectometry-mass spectometry (LC-MS-MS). Liver malondialdehyde (MDA) as marker of oxidative stress was measured.


RESULTS
t-Res accumulates in the liver reaching 49.8 +/- 10.2 ng/g after 7 days and 191.8 +/- 15.3 ng/g after 14 days. No t-Res was detected in serum. The increase of MDA liver concentration due to CCl4 injection after 24 h and 1 week was reduced by 38% and a 63%, respectively, by the treatment with the t-Res extract.


CONCLUSIONS
A moderate consumption of t-Res from a dietary source resulted in a time-dose-dependent liver accumulation. It was able to counteract in vivo CCl4-induced liver lipid peroxidation thus demonstrating the hepatoprotective property of t-Res.
Vitaglione
Paola
P
Department of Food Science, Gastroenterology Unit, Federico II University of Naples, Naples, Italy. paola.vitaglione@unina.it
Ottanelli
Barbara
B
Milani
Stefano
S
Morisco
Filomena
F
Caporaso
Nicola
N
Fogliano
Vincenzo
V
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000302:isolation & purification / Q000493:pharmacokinetics; D001682:Biological Availability; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury; D019610:Cytoprotection; D004032:Diet; D004195:Disease Models, Animal; D007441:Intubation, Gastrointestinal; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018547:Plant Stems; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage* / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics; D027843:Vitis / Q000737:chemistry
D000975:Antioxidants; D013267:Stilbenes; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D000077185:Resveratrol