Inhibition of HBV infection by bovine lactoferrin and iron-, zinc-saturated lactoferrin.
In this study, we investigated the effect of bovine lactoferrin (BLf), lactoferrin hydrolysate, or iron-, zinc-saturated lactoferrin on hepatitis B virus (HBV)-infected HepG2 cells. Fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to quantify HBV-DNA copies. BLf, iron- or zinc-saturated lactoferrin significantly inhibited the amplification of HBV-DNA in a dose-dependent manner in HBV-infected HepG2 cells. However, the inhibitive effect of lactoferrin hydrolysate on HBV-DNA copies was insignificant. These findings suggest that BLf inhibits the function of HBV by integrated structure. In conclusion, BLf, iron- or zinc-saturated BLf is one of the candidates for anti-HBV reagents in treatment of patients with hepatitis.
Li
Songtao
S
Northeast Agricultural University, 150030, Harbin, Heilongjiang, China.
Zhou
Haibo
H
Huang
Guirong
G
Liu
Ning
N
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D006515:Hepatitis B virus / Q000187:drug effects* / Q000502:physiology; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D007781:Lactoferrin / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004279:DNA, Viral; D007781:Lactoferrin