Induction of fibroblast growth factor-2 by electrical stimulation in cultured retinal Mueller cells.
It has been demonstrated that retinal Mueller cells, the predominant glial cells, produce neurotrophic factors including basic fibroblast growth factor (FGF-2), and that electrical stimulation enhances the transcription of FGF-2 in the central nervous system. In this study, the effect of electrical stimulation on the induction of FGF-2 in cultured rat Mueller cells was investigated by quantitative real-time polymerase chain reaction and western blotting. Both the messenger RNA and protein of FGF-2 were significantly upregulated after electrical stimulation compared with that of controls. These results suggest that electrical stimulation may directly induce the production of FGF-2 in the retina.
Sato
Tatsuhiko
T
Department of Applied Visual Science, Osaka University Medical School, Suita, Osaka, Japan.
Lee
Tong-Sheng
TS
Takamatsu
Fumihiko
F
Fujikado
Takashi
T
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004558:Electric Stimulation / Q000379:methods; D016222:Fibroblast Growth Factor 2 / Q000235:genetics* / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2