Microbead device for isolating biotinylated oligonucleotides for use in mass spectrometric analysis.
We describe a prototypical device for isolating biotinylated oligonucleotides for use in mass spectrometric analysis. It consists of monomeric avidin-coated microbeads trapped in a pipette tip and has been used for genotyping single nucleotide polymorphisms (SNPs) with the previously developed solid phase capture-single base extension (SPC-SBE) method. The device reduces processing time for genotyping by SPC-SBE and allows direct spotting of sample for rapid analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In addition, it allows simultaneous processing of multiple samples and can be reused after regeneration of beads with no carryover effects. These results indicate that the microbead device is a low-cost tool that enhances sample cleanup prior to MS for SNP genotyping.
Misra
Ashish
A
Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
Kim
Sobin
S
D019921:Biotinylation; D005838:Genotype; D013058:Mass Spectrometry / Q000379:methods*; D008863:Microspheres; D008954:Models, Biological; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D009841:Oligonucleotides