title: Role of linoleic Acid hydroperoxide preformed by cyclooxygenase-1 or -2 on the regulation of prostaglandin formation from arachidonic Acid by the respective enzyme.
abstract: Linoleic acid (LA) preincubated with cyclooxygenase (COX)-1 or -2 inhibited prostaglandin (PG) formation from arachidonic acid (AA) catalyzed by the respective enzyme, but LA without the preincubation did not. 13S-Hydroperoxy-9Z,11E-octadecadienoic acid (13-HPODE) a hydroperoxy adduct of LA inhibited PG formation catalyzed by COX-1 or -2. 13S-Hydroxy-9Z,11E-octadecadienoic acid had no effect on both COX-1 and -2 activities. These results suggest that 13-HPODE which is preformed from LA by COX reaction under normal physiological conditions can be a basal suppressor of PG formation from AA.
authors.affiliation: Department of Physiological Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Yohko
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Yonemura
authors.forename: Satoru
authors.initials: S
authors.lastname: Sakuma