A simple and rapid high-performance liquid chromatographic method for the determination of bisoprolol fumarate and hydrochlorothiazide in a tablet dosage form.
A simple and precise high performance liquid chromatographic method has been developed and validated for the simultaneous determination of bisoprolol fumarate (BF), and hydrochlorothiazide (HCTZ) in a tablet formulation. Chromatography was carried out at 25 degrees C on a 4.6 mm x 250 mm, 5 microm cyano column with the isocratic mobile phase of 0.1M aqueous phosphate buffer, acetonitrile and tetrahydrofuran (85:10:5, v/v/v) at a flow rate of 1.0 ml/min. The UV detection was carried out at 225 nm. HCTZ and BF were separated in less than 10 min with good resolution and minimal tailing, without interference of excipients. The method was validated according to ICH guidelines and the acceptance criteria for accuracy, precision, linearity, specificity and system suitability were met in all cases. The method was linear in the range of 50-150 microg/ml for BF and 125-375 microg/ml for HCTZ.
Shaikh
Saleem
S
Department of Analytical Research & Development, Jamjoom Pharmaceuticals, P.O. Box 6267, Jeddah 21442, Saudi Arabia. salim2001p@hotmail.com
Thusleem
O A
OA
Muneera
M S
MS
Akmal
Javeed
J
Kondaguli
Anand V
AV
Ruckmani
K
K
D000097:Acetonitriles / Q000737:chemistry; D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D017298:Bisoprolol / Q000032:analysis*; D002021:Buffers; D002740:Chlorothiazide / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004232:Diuretics / Q000032:analysis*; D004304:Dosage Forms; D004340:Drug Contamination; D005663:Furans / Q000737:chemistry; D017408:Guidelines as Topic; D006852:Hydrochlorothiazide / Q000032:analysis*; D010710:Phosphates / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D000319:Adrenergic beta-Antagonists; D002021:Buffers; D004232:Diuretics; D004304:Dosage Forms; D005663:Furans; D010710:Phosphates; D013607:Tablets; D014867:Water; D006852:Hydrochlorothiazide; C018674:tetrahydrofuran; D002740:Chlorothiazide; D017298:Bisoprolol; C032159:acetonitrile