Proteome analysis of multidrug resistance of human oral squamous carcinoma cells using CD147 silencing.
There is a correlation between the multidrug-resistance (MDR) of cancer cells and their enhanced invasive or metastatic potential. We studied the expression of CD147, a plasma membrane glycoprotein that plays a key role in tumor metastasis by stimulating the production of matrix metalloproteinases (MMPs), in sensitive human oral squamous KB and MDR derivative KB/V cells. Reverse transcription-PCR and flow cytometric analysis revealed that KB/V cells expressed CD147 at significantly higher levels than their parental KB cells. Using stable RNA interference, we succeeded in establishing a CD147 knock-down KB/V cell line (KB/VsiCD147). MTT colorimetric assay showed an increase in the chemosensitivity to vincristine (VCR), all transretinoic acid (ATRA), taxol, and 5-fluorouracil (5-Fu) of KB/VsiCD147 cells. Proteome analysis of KB, KB/V, and KB/VsiCD147 cell lines identified 21 differently expressed proteins. The enhanced expression of representative active proteins, GRP75 and CyPA, was confirmed by Western blotting and RT-PCR. In addition, pretreatment of KB/V cells with a CyPA-binding immunosuppressive drug, cyclosporine A (CsA), enhanced their chemosensitivity to VCR and 5-Fu. We document an abundance of molecules that interact with CD147 in the MDR of human oral squamous carcinoma cells. Additional studies are needed to investigate these novel target proteins of CD147.
Kuang
Ye-Hong
YH
Department of Dermatology, Xiang Ya Hospital, Central South University, Hunan, 410008, China, Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, 890-8520, Japan.
Chen
Xiang
X
Su
Juan
J
Wu
Li-Sha
LS
Li
Ji
J
Chang
Jing
J
Qiu
Ying
Y
Chen
Zhe-Sheng
ZS
Kanekura
Takuro
T
D051926:Basigin / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D004396:Coloring Agents / Q000378:metabolism; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005562:Formazans / Q000378:metabolism; D006801:Humans; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D014162:Transfection
D004396:Coloring Agents; D005562:Formazans; D020543:Proteome; D034741:RNA, Small Interfering; D013778:Tetrazolium Salts; D051926:Basigin; C079782:MTT formazan