A reassessment of the association between azulene and [60]fullerene. Possible pitfalls in the determination of binding constants through fluorescence spectroscopy.
We show here that the recently reported surprisingly large association constant (K = 7.6 x 10(4) M(-1)) between azulene and [60]fullerene is due to experimental artifacts, pointing out potential errors in the characterization of association equilibria by fluorescence spectroscopy, and suggesting the best experimental practices.
Stella
Lorenzo
L
Dipartimento di Scienze e Tecnologie Chimiche, Università "Tor Vergata", via della Ricerca Scientifica 1, 00133, Roma, Italy. stella@stc.uniroma2.it
Capodilupo
Agostina L
AL
Bietti
Massimo
M
D052176:Azulenes / Q000737:chemistry*; D001665:Binding Sites; D001667:Binding, Competitive; D037741:Fullerenes / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012995:Solubility; D012996:Solutions / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D014050:Toluene / Q000737:chemistry
D052176:Azulenes; D037741:Fullerenes; D012996:Solutions; D014050:Toluene; C005525:azulene; C069837:fullerene C60