title: [Global expression profiling of Saccharomyces cerevisiae: metabolic remodeling in post-log phase].
abstract: For the purpose of revealing the mechanism of the reduction of yeasts ethanol production rate after entrance of post-log phase, we used microarray to study expression profiles of the yeast Saccharomyces cerevisiae during the transition from mid-log growth phase to post-log growth. The results demonstrate that the global pattern of gene expression is very stable during the mid-log phase. However, a dramatic metabolic remodeling was found when the yeast entries post-log phase, during which many of amino acid synthesis and metabolism related genes are up-regulated, moreover, ion transport, energy generation and storage related genes are also up regulated during this phase, while a large number of genes involved in transposition and DNA recombination are repressed. Central metabolic pathways also engage in metabolic remodeling, within which the genes involved in succinate and a-ketoglutarate synthesis pathways are up regulated, accordance with those of amino acid synthesis and metabolism. These results demonstrate that the increasing demand for amino acids in post-log phase lead to a metabolic transition into TCA cycle and glyoxylate cycle, which subsequently reduce the ethanol production rate. This suggests a global insight into the process of yeast ethanol fermentation.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Yanrui
authors.initials: Y
authors.lastname: Ye
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Tang
authors.forename: Hongyun
authors.initials: H
authors.lastname: Chen
authors.forename: Suiping
authors.initials: S
authors.lastname: Zheng
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis; D000431:Ethanol / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D007656:Ketoglutaric Acids / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D000596:Amino Acids; D007656:Ketoglutaric Acids; D000431:Ethanol; D019802:Succinic Acid