Plastinated tissue samples as three-dimensional models for optical instrument characterization.
Histology of biological specimens is largely limited to investigating two-dimensional structure because of the sectioning required to produce optically thin samples for conventional microscopy. With the advent of three-dimensional optical imaging technologies such as optical coherence tomography (OCT), diffuse optical tomography (DOT), and multiphoton microscopy (MPM), methods of tissue preparation that minimally disrupt three-dimensional structure are needed. We propose plastination as a means of transforming tissues into three-dimensional models suitable for optical instrument characterization. Tissues are plastinated by infusing them with transparent polymers, after which they can be safely handled, unlike fresh or fixed tissues. Such models are useful for investigating three-dimensional structure, testing and comparing the performance of optical instruments, and potentially investigating tissue properties not normally observed after the three-dimensional scattering properties of a biological samples are lost. We detail our plastination procedures and show examples of imaging several plastinated tissues from a pre-clinical rat model using optical coherence tomography.
Marks
Daniel L
DL
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Ave., Urbana, IL 61801, USA.
Chaney
Eric J
EJ
Boppart
Stephen A
SA
D000818:Animals; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D007089:Image Enhancement / Q000379:methods; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D008853:Microscopy / Q000295:instrumentation; D055095:Optics and Photonics; D011108:Polymers / Q000737:chemistry; D051381:Rats; D012825:Silicon / Q000737:chemistry; D012828:Silicones / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D014018:Tissue Distribution; D041623:Tomography, Optical Coherence / Q000295:instrumentation*
D004129:Dimethylpolysiloxanes; D011108:Polymers; D012828:Silicones; D012997:Solvents; C013830:baysilon; D012825:Silicon