title: Conserved properties of a urochordate estrogen receptor-related receptor (ERR) with mammalian ERRalpha.
abstract: Estrogen receptor-related receptors (ERRs) were the first orphan nuclear receptors identified on the basis of their sequence similarity to the estrogen receptors. Although unique ERRs were found in some marine invertebrates, the molecular functions of these receptors are not well understood. In the present study, we identified three transcript variants of the tunicate Halocynthia roretzi ERR (Hr-ERR), varying in their 3' untranslated regions, and putatively encoding a unique receptor deriving from an ancestor protein common to vertebrate ERRalpha/beta/gamma. Maternal mRNA of Hr-ERR was detected throughout the entire egg cytoplasm and early embryos. Zygotic Hr-ERR was predominantly expressed in the heart, and at lower levels in muscle, stomach, gonad and digestive glands. Electrophoretic mobility shift assay demonstrated that Hr-ERR directly binds to the estrogen-response element (ERE) and ERR-response element (ERRE). Gene reporter assays also showed that Hr-ERR activates transcription through ERE and ERRE. Hr-ERR-mediated transactivation was modulated by various cofactors for mammalian ERRs, such as peroxisome proliferator-activated receptor gamma coactivator 1-alpha and small heterodimer partner. In addition, the ERR antagonists 4-hydroxytamoxifen and diethylstilbestrol inhibited the Hr-ERR-mediated transactivation, whereas Hr-ERR activity on ERE was further induced by genistein, an ERRalpha agonist. Taken together, our results show that Hr-ERR is an unduplicated ERR that however, possesses functional properties common to ERRalpha and not to ERRbeta/gamma.
authors.affiliation: Faculty of Marine Bioscience and Technology, Kangnung National University, Gangneung 210-702, Republic of Korea.
authors.forename: WooDong
authors.initials: W
authors.lastname: Park
authors.forename: Gil Jung
authors.initials: GJ
authors.lastname: Kim
authors.forename: Hueng-sik
authors.initials: HS
authors.lastname: Choi
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Vanacker
authors.forename: Young Chang
authors.initials: YC
authors.lastname: Sohn
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D024202:Electrophoretic Mobility Shift Assay; D006367:HeLa Cells; D006801:Humans; D008322:Mammals / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011960:Receptors, Estrogen / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014561:Urochordata / Q000378:metabolism*; D000097983:ERRalpha Estrogen-Related Receptor
chemical_list: D011960:Receptors, Estrogen; D000097983:ERRalpha Estrogen-Related Receptor