Identification of shark species in seafood products by forensically informative nucleotide sequencing (FINS).
The identification of commercial shark species is a relevant issue to ensure the correct labeling of seafood products, to maintain consumer confidence in seafood, and to enhance the knowledge of the species and volumes that are at present being captured, thus improving the management of shark fisheries. The polymerase chain reaction was employed to obtain a 423 bp amplicon from the mitochondrial cytochrome b gene. The sequences from this fragment, belonging to 63 authentic individuals of 23 species, were analyzed using a genetic distance method. Nine different samples of commercial fresh, frozen, and convenience food were obtained in local and international markets to validate the methodology. These samples were analyzed, and sequences were employed for species identification, showing that forensically informative nucleotide sequencing (FINS) is a suitable technique for identification of processed seafood containing shark as an ingredient. The results also showed that incorrect labeling practices may occur regarding shark products, probably because of incorrect labeling at the production point.
Blanco
M
M
Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Eduardo Cabello 6, 36208 Vigo, Spain. mblanco@iim.csic.es
Pérez-Martín
R I
RI
Sotelo
C G
CG
D000818:Animals; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics; D005515:Food Labeling; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017747:Seafood / Q000032:analysis*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012754:Sharks / Q000145:classification / Q000235:genetics*
D029941:Fish Proteins; D045303:Cytochromes b