[Determination of plasma protein binding rate of methyl protodioscin with ultrafiltration].
OBJECTIVE
To study the plasma protein binding rate of methyl protodioscin.


METHODS
The ultrafiltration was employed to determine the plasma protein binding rate of methyl protodioscin. The plasma concentrations of methyl protodioscin were measured by HPLC-MS-MS.


RESULTS
The plasma protein binding rate of methyl protodioscin with rat plasma at the concentration of 20.0, 100 and 200 microg x mL(-1) were (94.6 +/- 0.16)%, (91.6 +/- 0.35)% and (86.10 +/- 0.60)%, respectively, while the plasma protein binding rate of methyl protodioscin with normal human plasma at the above concentrations were (82.11 +/- 5.12)%, (84.54 +/- 0.32)% and (88.52 +/- 1.02)%, respectively.


CONCLUSIONS
The binding rate of methyl protodioscin with plasma protein is high.
Yao
Zhi-Hong
ZH
Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China.
Cao
Xiu-Zhen
XZ
Shao
Meng
M
Pan
Yu-Ming
YM
Ye
Wen-Cai
WC
Yao
Xin-Sheng
XS
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D011485:Protein Binding; D051381:Rats; D012503:Saponins / Q000378:metabolism*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D014462:Ultrafiltration
D000970:Antineoplastic Agents; D001798:Blood Proteins; D012503:Saponins; C425324:methyl protodioscin; D004144:Diosgenin