Immune-relevant genes expressed in rainbow trout following immunisation with a live vaccine against Ichthyophthirius multifiliis.
Rainbow trout Oncorhynchus mykiss were immunised by intra-peritoneal injection using a live vaccine based on Ichthyophthirius multifiliis (Ich) theronts, which previously has shown protection against white spot disease. Samples were taken pre-vaccination and on Day 1, 7, 21 and 28 post-immunisation (p.i.). Expression of immune relevant genes in the liver, spleen and head kidney was monitored by qPCR. To describe the immune reaction following this immunisation, a series of genes encoding cytokines, complement factors, immunoglobulins and acute phase reactants were studied. Genes encoding acute phase reactants in the liver were up-regulated with serum amyloid A (SAA) as the most pronounced with a 2299-fold increase at 24 h p.i. Hepcidin and pre-cerebellin were also up-regulated in the liver 24 h p.i., by 7- and 4-fold, respectively. Complement factors C3, C5 and factor B (Bf) were up-regulated in the spleen and the head kidney 24 h and 28 d p.i. Genes encoding immunoglobulins were not up-regulated, but a specific low titer IgM response (titer 25) against parasite antigens was detected by a modified ELISA 4 wk p.i.
von Gersdorff Jorgensen
Louise
L
Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen, Stigbøjlen 7, 1870 Frederiksberg C., Denmark. lgj@life.ku.dk
Nemli
Egemen
E
Heinecke
Rasmus D
RD
Raida
Martin K
MK
Buchmann
Kurt
K
D000209:Acute-Phase Proteins / Q000235:genetics; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D016798:Ciliophora / Q000276:immunology* / Q000472:pathogenicity; D016770:Ciliophora Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D003165:Complement System Proteins / Q000235:genetics; D016207:Cytokines / Q000235:genetics; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control*; D005786:Gene Expression Regulation / Q000276:immunology*; D007114:Immunization / Q000379:methods / Q000662:veterinary*; D007136:Immunoglobulins / Q000235:genetics; D007274:Injections, Intraperitoneal / Q000662:veterinary; D017686:Oncorhynchus mykiss / Q000276:immunology* / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D016052:Protozoan Vaccines / Q000008:administration & dosage; D014613:Vaccines, Attenuated / Q000008:administration & dosage
D000209:Acute-Phase Proteins; D000913:Antibodies, Protozoan; D016207:Cytokines; D007136:Immunoglobulins; D016052:Protozoan Vaccines; D014613:Vaccines, Attenuated; D003165:Complement System Proteins