Effect of iron-quercetin complex on reduction of nitrite in in vitro and in vivo systems.
This study investigated whether reducing agents such as quercetin and iron(II) facilitate formation of nitric oxide (NO) gas from orally ingested nitrite in an vivo study. When 3 mg/kg Na (15)NO2 was orally administered to rats with or without iron(II) or quercetin, Hb (15)NO, which is indicative of systemic (15)NO, was detected in the blood, with the maximum blood concentration of Hb (15)NO at 15 min after nitrite or nitrite plus quercetin treatment, whereas after administration of nitrite plus iron(II) or nitrite plus iron(II) and quercetin, the time was shortened to 10 min. Interestingly, iron(II), quercetin, or iron(II) plus quercetin did not affect the total amount of Hb (15)NO generated from orally administered Na (15)NO2. However, the systemic nitrite concentration was significantly decreased in the presence of iron(II) or iron(II) plus quercetin. These results may indicate that iron(II) is critical to the generation of NO gas from nitrite, whereas quercetin contributed little under the in vivo experimental conditions.
Ohnishi
Hideki
H
Department of Pharmacology, The University of Tokushima Graduate School, 1-78-1 Sho-machi, Tokushima 770-8505, Japan.
Iwanaga
Satoshi
S
Kawazoe
Kazuyoshi
K
Ishizawa
Keisuke
K
Orino
Sakiko
S
Tomita
Shuhei
S
Tsuchiya
Koichiro
K
Kanematsu
Yasuhisa
Y
Harada
Nagakatsu
N
Mori
Kazuhiro
K
Tsuchihashi
Tomoko
T
Ishikawa
Yasuko
Y
Tamaki
Toshiaki
T
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009566:Nitrates / Q000737:chemistry / Q000378:metabolism; D009569:Nitric Oxide / Q000737:chemistry / Q000378:metabolism; D009573:Nitrites / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011794:Quercetin / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; D011794:Quercetin; D007501:Iron