Supercritical fluid extraction of aflatoxin B(1) from soil.
This research describes the development of a supercritical fluid extraction (SFE) method to recover aflatoxin B(1) from fortified soil. The effects of temperature, pressure, modifier (identity and percentage), and extraction type were assessed. Using the optimized SFE conditions, the mean recovery from air dried soil was 72%. The variables associated with changes in recovery of aflatoxin were co-solvents, static extraction, and temperature. Acetonitrile-2% acetic acid, used both in-cell and on-line, provided the most efficient recovery. The results indicate that desorption from the soil was the limiting factor in recovery and that the static phase was more important than the dynamic.
Starr
James M
JM
US Environmental Protection Agency, National Exposure Research Laboratory, USEPA MD D205-05, 109 T.W. Alexander Drive, Research Triangle Park, NC 27711, USA. starr.james@epa.gov
Selim
Mustafa I
MI
D016604:Aflatoxin B1 / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D015203:Reproducibility of Results; D012989:Soil Pollutants / Q000032:analysis / Q000302:isolation & purification*
D012989:Soil Pollutants; D016604:Aflatoxin B1