title: Polymorphisms of the endothelial nitric oxide synthase gene in premenopausal women with polycystic ovary syndrome.
abstract: OBJECTIVE
  To investigate the association of two common genetic polymorphisms of the gene encoding for endothelial nitric oxide synthase (Nos3), the enzyme catalyzing the production of nitric oxide (NO), with occurrence of the polycystic ovary syndrome (PCOS).
  
  
  METHODS
  In a prospective case-control study, we analyzed 2 polymorphisms of the Nos3 gene cluster (Nos 3 exon 7 Glu298Asp and 27-base pair repeat in intron 4 of Nos3) in a series of 210 premenopausal Caucasian women with PCOS and 171 healthy controls using pyrosequencing and PCR, respectively. Women completed a detailed questionnaire and underwent a peripheral venous puncture, ultrasonography, and a standardized oral glucose tolerance test (OGTT).
  
  
  RESULTS
  Genotype frequencies were not significantly different among women with PCOS and controls for the exon 7Nos3 and the intron 4Nos3 polymorphism (p=0.3 and 0.2, respectively).
  
  
  CONCLUSIONS
  In our series, two common polymorphisms of the Nos3 gene cluster were not associated with occurrence of PCOS.
authors.affiliation: Elisabeth Vytiska-Binstorfer, Johannes Huber, Konstantin Baumühlner, René Wenzl, Fritz Nagele, Lukas Hefler, Austria. katharina.walch@meduniwien.ac.at
authors.forename: Katharina
authors.initials: K
authors.lastname: Walch
authors.forename: Andrea
authors.initials: A
authors.lastname: Kolbus
authors.forename: Katrin
authors.initials: K
authors.lastname: Hefler-Frischmuth
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D005951:Glucose Tolerance Test; D006801:Humans; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011085:Polycystic Ovary Syndrome / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D018709:Statistics, Nonparametric
chemical_list: D004247:DNA; D052250:Nitric Oxide Synthase Type III