title: Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage.
abstract: Polymeric medical devices widely used in orthopedic surgery play key roles in fracture fixation and orthopedic implant design. Topographical modification and surface micro-roughness of these devices regulate cellular adhesion, a process fundamental in the initiation of osteoinduction and osteogenesis. Advances in fabrication techniques have evolved the field of surface modification; in particular, nanotechnology has allowed the development of nanoscale substrates for the investigation into cell-nanofeature interactions. In this study human osteoblasts (HOBs) were cultured on ordered nanoscale pits and random nano "craters" and "islands". Adhesion subtypes were quantified by immunofluorescent microscopy and cell-substrate interactions investigated via immuno-scanning electron microscopy. To investigate the effects of these substrates on cellular function 1.7 k microarray analysis was used to establish gene profiles of enriched STRO-1+ progenitor cell populations cultured on these nanotopographies. Nanotopographies affected the formation of adhesions on experimental substrates. Adhesion formation was prominent on planar control substrates and reduced on nanocrater and nanoisland topographies; nanopits, however, were shown to inhibit directly the formation of large adhesions. STRO-1+ progenitor cells cultured on experimental substrates revealed significant changes in genetic expression. This study implicates nanotopographical modification as a significant modulator of osteoblast adhesion and cellular function in mesenchymal populations.
authors.affiliation: Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G128QQ, United Kingdom. m.biggs.1@research.gla.ac.uk
authors.forename: Manus J P
authors.initials: MJ
authors.lastname: Biggs
authors.forename: R Geoff
authors.initials: RG
authors.lastname: Richards
authors.forename: Nikolaj
authors.initials: N
authors.lastname: Gadegaard
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: McMurray
authors.forename: Stanley
authors.initials: S
authors.lastname: Affrossman
authors.forename: Chris D W
authors.initials: CD
authors.lastname: Wilkinson
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
authors.forename: Mathew J
authors.initials: MJ
authors.lastname: Dalby
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D051177:Catenins / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D051177:Catenins; D054377:Chemokine CXCL12; D051153:Wnt Proteins