title: Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil.
abstract: E. faecium was the first reported VRE species, carrying the vanA gene in Brazil. In spite of this, vancomycin-resistant E. faecalis has become the predominant species in Brazilian hospitals. The aim of this study was to evaluate the genetic relatedness of VREs isolated in a Brazilian teaching hospital eight years apart from its first isolation. We analyzed 38 VRE strains obtained from 81 surveillance cultures of patients admitted to the four largest intensive care units in Hospital São Paulo in February, 2006. Presence of the vanA gene was assayed by PCR and PFGE analysis was used for molecular characterization. All VRE strains carried the vanA gene. Two distinct clonal groups were observed among vancomycin-resistant E. faecalis. Vancomycin-resistant E. faecium belonged to five distinct clones were demonstrated by molecular typing. All of these clones were different from the first vancomycin-resistant enterococci clone isolated eight years ago in our hospital.
authors.affiliation: Laboratório Especial de Microbiologia Clínica (LEMC) da Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brasil. pedro_dazevedo@yahoo.com.br
authors.forename: Pedro Alves
authors.initials: PA
authors.lastname: d'Azevedo
authors.forename: Guilherme H C
authors.initials: GH
authors.lastname: Furtado
authors.forename: Eduardo A S
authors.initials: EA
authors.lastname: Medeiros
authors.forename: Kelly Aline
authors.initials: KA
authors.lastname: Santiago
authors.forename: Suzane
authors.initials: S
authors.lastname: Silbert
authors.forename: Antonio C C
authors.initials: AC
authors.lastname: Pignatari
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006801:Humans; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020713:Vancomycin Resistance / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C071437:VanA ligase, Bacteria; D019729:Carbon-Oxygen Ligases