title: Nanoparticle self-assembly on a DNA-scaffold written by single-molecule cut-and-paste.
abstract: Self-assembly guided by molecular recognition has in the past been employed to assemble nanoparticle superstructures like hypercrystals or nanoparticle molecules. An alternative approach, the direct molecule-by-molecule assembly of nanoscale superstructures, was demonstrated recently. Here we present a hybrid approach where we first assemble a pattern of binding sites one-by-one at a surface and then allow different nanoparticles to attach by self-assembly. For this approach, biotin bearing DNA oligomers were picked up from a depot using a cDNA strand bound to an AFM tip. These units were deposited in the target area by hybridization, forming a recognition pattern on this surface. Fluorescent semiconductor nanoparticles conjugated with streptavidin were allowed to assemble on this scaffold and to form the final nanoparticle superstructures.
authors.affiliation: Chair for Applied Physics, Ludwig-Maximilians-Universität Munich, Amalienstrasse 54, 80799 Munich.
authors.forename: Elias M
authors.initials: EM
authors.lastname: Puchner
authors.forename: Stefan K
authors.initials: SK
authors.lastname: Kufer
authors.forename: Mathias
authors.initials: M
authors.lastname: Strackharn
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Stahl
authors.forename: Hermann E
authors.initials: HE
authors.lastname: Gaub
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D015342:DNA Probes / Q000737:chemistry; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D015342:DNA Probes; D004247:DNA