title: Collagen XVI expression is upregulated in glioblastomas and promotes tumor cell adhesion.
abstract: The poor prognosis of glioblastoma patients is related to diffuse brain invasion and interaction of tumor cells with extracellular matrices (ECM). We describe expression and function of the FACIT-collagen XVI in glioblastomas. We found upregulation of collagen XVI mRNA as well as protein in glioblastomas as compared to normal cortex. SiRNA knockdown resulted in decreased cell adhesion whereas increased adhesion was observed on surfaces coated with collagen XVI. The migration of glioblastoma cells on this substrate remained unchanged. Our results demonstrate de-novo expression of collagen XVI in glioblastomas as part of the tumor specific remodeling of the ECM.
authors.affiliation: Institute of Neuropathology, University Hospital Muenster, Domagkstrasse 19, D-48149 Muenster, Germany. senner@uni-muenster.de
authors.forename: Volker
authors.initials: V
authors.lastname: Senner
authors.forename: Sabine
authors.initials: S
authors.lastname: Ratzinger
authors.forename: Sonja
authors.initials: S
authors.lastname: Mertsch
authors.forename: Susanne
authors.initials: S
authors.lastname: Grässel
authors.forename: Werner
authors.initials: W
authors.lastname: Paulus
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D005109:Extracellular Matrix / Q000378:metabolism; D024021:Fibril-Associated Collagens / Q000096:biosynthesis* / Q000235:genetics; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D015854:Up-Regulation
chemical_list: C481130:COL16A1 protein, human; D024021:Fibril-Associated Collagens; D003094:Collagen