End-functionalized phosphorylcholine methacrylates and their use in protein conjugation.
Polymer-protein conjugation was performed using N-hydroxysuccinimide and aldehyde-terminated zwitterionic polymers, and the resulting polymer-protein conjugates were characterized by gel electrophoresis and fast protein liquid chromatography. Methacryloyloxyethyl phosphorylcholine (MPC) polymers were prepared by atom transfer radical polymerization in which the requisite functional end-groups for protein conjugation were embedded within the polymerization initiators. These phosphorylcholine polymers were conjugated to lysozyme as a model protein, as well as two therapeutic proteins, granulocyte colony stimulating factor (G-CSF) and erythropoietin (EPO). These MPC polymer-protein conjugates represent alternatives to PEGylated proteins, with the potential to provide improved efficacy in a therapeutic treatment relative to the protein itself.
Samanta
Debasis
D
Polymer Science and Engineering Department, University of Massachusetts, 120 Governors Drive, Amherst, Massachusetts 01003, USA.
McRae
Samantha
S
Cooper
Beth
B
Hu
Yunxia
Y
Emrick
Todd
T
Pratt
Jeanne
J
Charles
Stephen A
SA
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D004921:Erythropoietin / Q000737:chemistry; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry*; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry; D010767:Phosphorylcholine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
D001672:Biocompatible Materials; D046911:Macromolecular Substances; D008689:Methacrylates; D011108:Polymers; D011506:Proteins; D010767:Phosphorylcholine; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D009113:Muramidase