Toward understanding the mechanism of improving the production of volatile fatty acids from activated sludge at pH 10.0.
A well-defined fractionation approach for sludge flocs was applied to a better understanding of the underlying mechanism of improving the production of volatile fatty acids (VFA) in the hydrolysis and acidification processes at pH 10.0. Specifically, sludge flocs were fractionated through centrifugation and ultrasound into four fractions: (1) slime, (2) loosely bound extracellular polymeric substances (LB-EPS), (3) tightly bound EPS (TB-EPS) and (4) pellet. Result showed that during 20 days of fermentation, the total VFA production at pH 10.0 was higher, from 2 to 34 times, than that at pH 5.5. At pH 10.0, however, the enzyme activities (i.e. protease, alpha-amylase, alkaline phosphatase and acid phosphatase) in all fractions of sludge flocs were all lower than pH 5.5, which strongly suggests that the biotic effect was not the leading cause of the VFA improvement. Further investigation suggests that pH 10.0 could significantly improve the VFA production through the break of sludge matrix which is usually hydrolyzed by the extracellular enzymes embedded in itself, increase the effective contact between extracellular organic matters and enzymes, and create a favorable environment for microbes to accumulate VFA. Hydrolysis and acidification at pH 10.0 can be considered as part of an appropriate solution for tertiary treatment and contribute to the headway toward the goal of sustainable water treatment technologies.
Yu
Guang-Hui
GH
State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, Siping Road No. 1239, Shanghai, P.R. China.
He
Pin-Jing
PJ
Shao
Li-Ming
LM
He
Pei-Pei
PP
D005232:Fatty Acids, Volatile / Q000737:chemistry* / Q000378:metabolism; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D018508:Water Purification / Q000379:methods
D005232:Fatty Acids, Volatile; D012722:Sewage