Analyses of differential proteome of human synovial fibroblasts obtained from arthritis.
There is mounting evidence indicating that the synovial fibroblasts (SFs) contribute to the pathogenesis of rheumatoid arthritis (RA). The present study showed the differential proteins expression pattern of SFs from patients with RA or osteoarthritis (OA) and healthy control. Cellular proteins of cultured SFs were subjected to 2-DE and visualized by silver nitrate staining. A total of 49 spots that were statistically and differentially overexpressed in RA or OA in comparison to healthy ones were identified by MALDI-TOF-MS, and 25 proteins were successfully identified. Western blot was used to further verify some of the differential proteins. These proteins included enzymatic and structural proteins, signal transduction proteins, calcium binding protein, etc. From all of the identified proteins, a number of proteins have been implicated that involved in the healthy or pathological SFs function (e.g., S100A4, S100A10, cathepsin D) or that have potential diagnostic and prognostic value for RA (alpha-enolase and TPI) or that may be the new therapeutic targets (Annexin, SOD, PRX).
Bo
Gan-Ping
GP
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Zhou
Li-Na
LN
He
Wei-Feng
WF
Luo
Gao-Xing
GX
Jia
Xiong-Fei
XF
Gan
Cheng-Jun
CJ
Chen
Guang-Xing
GX
Fang
Yong-Fei
YF
Larsen
Peter Mose
PM
Wu
Jun
J
D017306:Annexin A2 / Q000378:metabolism; D017302:Annexins / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000378:metabolism* / Q000473:pathology; D001706:Biopsy; D016022:Case-Control Studies; D002402:Cathepsin D / Q000378:metabolism; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D010003:Osteoarthritis / Q000378:metabolism* / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013583:Synovial Membrane / Q000473:pathology*; D000099248:S100 Calcium Binding Protein A10
D017306:Annexin A2; D017302:Annexins; D000954:Antigens, Surface; D002402:Cathepsin D; D019204:GTP-Binding Proteins; D010751:Phosphopyruvate Hydratase; D020543:Proteome; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10; C077591:S100A4 protein, human; C483942:SPAG1 protein, human