Comparison of two stationary phases for the separation of five selected polyphenols.
In this study, a conventional TEKNOKROMA, Tr-015605 TRACER EXTRASIL ODS2 (25 cm x 0.4 cm, i.d. 5 microm) column and a new stationary phase TEKNOKROMA, Tr-010065 Mediterranea sea(18) (15 cm x 0.4 cm, i.d. 3 microm) were compared for the separation of five phenolic compounds. Sensitivity, performance characteristics, analysis time and method validation were reported for both columns. A better sensitivity and performance were achieved with the novel chromatographic support. In addition the total analysis time was significantly reduced. The method was applied to the determination of the selected polyphenols in commercial available white wines. LC-MS was used as a confirmatory technique.
Rodríguez-Bernaldo de Quirós
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A
Analytical Chemistry, Nutrition and Bromatology Department, Pharmacy Faculty, Campus Sur s/n, University of Santiago de Compostela, 15782 Santiago de Compostela (La Coruña), Spain. anarbq@usc.es
Lage-Yusty
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MA
López-Hernández
J
J
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D019800:Phenol; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D059808:Polyphenols; D021241:Spectrometry, Mass, Electrospray Ionization
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D019800:Phenol