Controlled release of complexed DNA from polycaprolactone film: comparison of lipoplex and polyplex release.
This report investigates the comparative in vitro controlled release and transfection efficiencies of pDNA-lipofectamine complex (lipoplex) and pDNA-poly(ethylene imine) complex (polyplex), from a biodegradable polycaprolactone (PCL) film. The effect of molecular weight of gelatin used as a porogen on in vitro release and transfection efficiency was also studied. A sustained release profile was obtained for naked pDNA and lipoplex from polymeric films for a month, while the release of polyplexes (PEI/DNA) is simply a burst at day 5, with little or no release thereafter. The release of polyplexes from PCL films is retarded due to interaction between the polyplexes and the polymer. A high burst release was seen for naked pDNA which was suppressed in the presence of gelatin. The extent of suppression of the burst effect by gelatin increased with its molecular weight. For complexed pDNA (lipoplex), the release was slow, but could be accelerated using gelatin; again the acceleration in release is dependant on the molecular weight of the gelatin used. The addition of gelatin as a porogen has no effect on the release of polyplexes from PCL films. The bioactivity of released plasmid DNA and complexes was studied by in vitro transfection using COS-7 cells. Transfection was observed from released lipoplexes samples till day 9 from PCL film with lower MW gelatin and till day 18 in the case of PCL films with higher MW gelatin. The results also showed that the bioactivity of released lipoplexes was superior to that of the naked pDNA.
Ramgopal
Y
Y
Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, Singapore 637819.
Mondal
D
D
Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, Singapore 637819.
Venkatraman
S S
SS
Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, Singapore 637819.
Godbey
W T
WT
Department of Chemical and Biomolecular Engineering, Tulane University, New Orleans, Louisiana 70118.
Yuen
G Y
GY
Department of Natural Sciences and Science Education, National Institute of Education, Nanyang Technological University, Singapore 637616.
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry / Q000378:metabolism; D007202:Indicators and Reagents / Q000737:chemistry; D008055:Lipids; D008081:Liposomes / Q000737:chemistry*; D008422:Materials Testing; D015394:Molecular Structure; D008970:Molecular Weight; D011091:Polyesters / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014162:Transfection
D001672:Biocompatible Materials; D004337:Drug Carriers; D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin; D011094:Polyethyleneimine; D004247:DNA