title: Histamine release inhibitory activity of Piper nigrum leaf.
abstract: Oral administration of a methanolic extract of Piper nigrum leaf (PN-ext, 50, 200 and 500 mg/kg) showed a potent dose-dependent inhibition of dinitrofluorobenzene (DNFB)-induced cutaneous reaction at 1 h [immediate phase response (IPR)] after and 24 h [late phase response (LPR)] after DNFB challenge in mice which were passively sensitized with anti-dinitrophenyl (DNP) IgE antibody. Ear swelling inhibitory effect of PN-ext (50, 200 and 500 mg/kg, per os (p.o.)) on very late phase response (vLPR) in the model mice was significant but weaker than that on IPR. Oral administration of PN-ext (50, 200 and 500 mg/kg for 7 d) inhibited picryl chloride (PC)-induced ear swelling in PC sensitized mice. PN-ext exhibited in vitro inhibitory effect on compound 48/80-induced histamine release from rat peritoneal mast cells. Two lignans of PN-ext, (-)-cubebin (1) and (-)-3,4-dimethoxy-3,4-desmethylenedioxycubebin (2), were identified as major active principles having histamine release inhibitory activity.
authors.affiliation: School of Pharmacy, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Hirata
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Naruto
authors.forename: Kazunori
authors.initials: K
authors.lastname: Inaba
authors.forename: Kimihisa
authors.initials: K
authors.lastname: Itoh
authors.forename: Masashi
authors.initials: M
authors.lastname: Tokunaga
authors.forename: Munekazu
authors.initials: M
authors.lastname: Iinuma
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D003877:Dermatitis, Contact / Q000517:prevention & control; D005260:Female; D006636:Histamine Release / Q000187:drug effects*; D006969:Hypersensitivity, Immediate / Q000517:prevention & control; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D010853:Picryl Chloride; D029222:Piper nigrum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: C501790:3,4-dimethoxy-3,4-desmethylenedioxycubebin; D017705:Lignans; D010936:Plant Extracts; D003189:p-Methoxy-N-methylphenethylamine; C433065:cubebin; D010853:Picryl Chloride