title: The influence of different hormone concentration and combination on callus induction and regeneration of Rauwolfia serpentina L. Benth.
abstract: The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L(-1) BA and 2.0 mg L(-1) NAA. The callus differentiated into adventitious shoots when it was subcultured on MS medium supplemented with 2.0 mg L(-1) BA with 0.2 mg L(-1) NAA. Regenerated shoots were best rooted on half-strength MS medium with 1.0 mg L(-1) each of IBA and IAA.
authors.affiliation: Department of RDDR, Modern Herbal Group, Dhaka-1217, Bangladesh.
authors.forename: U
authors.initials: U
authors.lastname: Salma
authors.forename: M S M
authors.initials: MS
authors.lastname: Rahman
authors.forename: S
authors.initials: S
authors.lastname: Islam
authors.forename: N
authors.initials: N
authors.lastname: Haque
authors.forename: T A
authors.initials: TA
authors.lastname: Jubair
authors.forename: A K M F
authors.initials: AK
authors.lastname: Haque
authors.forename: I J
authors.initials: IJ
authors.lastname: Mukti
mesh_terms: D000470:Alkaloids / Q000737:chemistry; D002450:Cell Communication / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D005838:Genotype; D006863:Hydrogen-Ion Concentration; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D011926:Rauwolfia / Q000187:drug effects* / Q000502:physiology; D012038:Regeneration / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D000470:Alkaloids; D010937:Plant Growth Regulators