title: In vitro examination of degenerative evolution of adrenergic nerve endings in pulmonary inflammatory [corrected] processes in newborns.
abstract: Morphological aspect of tracheal preparations and pulmonary tissue was studied in vitro. The material was obtained from autopsy of newborns that died from different causes. Examinations were made in different gestational periods (immature 23-29 weeks; premature 30-37 weeks; mature >38 weeks). Material for examination was obtained up to 6 hours after death. Pulmonary and tracheal tissue was incubated for fixation in buffered formalin (10%). Special histochemical and histoenzymatic methods were used for coloring of pulmonary and tracheal tissue and the activity of ATP-ase and dopaoxidase was monitored. Cut out models were made in series of 7 micro, 10 micro and 20 micro. In peripheral axons of tracheobronchial pathways, degenerative alterations of adrenergic nerve endings in lung inflammatory processes were documented. These morphologic neuronal changes were described: Walerians degeneration, neuro-axonal degeneration and segment demyelinisation. These changes are well seen with argentafine coloring (Sevier-Munger modification for nerve endings) and with dopaoxidase reaction. In mature newborns that died from respiratory distress syndrome, we found different forms of metabolic and toxic degenerative damage in peripheral axons, such as: segment demyelinisation, neurotubular fragmentation, Schwann cell proliferation, fragmentation and bulging out of axonal neurotubules and neurofilaments. In tracheo-bronchial tissue, chromafine granules are homogeneously distributed on Lamina propria layer and through glandular structures. This gives as a contradiction, according to some authors, that adrenergic nerve fibers for muscle tissue are absent and that adrenaline and noradrenaline diffuse in muscle tissue from interstice.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Prishtina, Clinical Centre N.N. 10000, Pristhina, Kosovo.
authors.forename: Hilmi
authors.initials: H
authors.lastname: Islami
authors.forename: Ragip
authors.initials: R
authors.lastname: Shabani
authors.forename: Naim
authors.initials: N
authors.lastname: Haliti
authors.forename: Sadi
authors.initials: S
authors.lastname: Bexheti
authors.forename: Rozafa
authors.initials: R
authors.lastname: Koliqi
authors.forename: Denis
authors.initials: D
authors.lastname: Raka
authors.forename: Aziz
authors.initials: A
authors.lastname: Sukalo
authors.forename: Rusmi
authors.initials: R
authors.lastname: Izairi
authors.forename: Hilmi
authors.initials: H
authors.lastname: Dauti
authors.forename: Nazim
authors.initials: N
authors.lastname: Qehaja
mesh_terms: D000320:Adrenergic Fibers / Q000473:pathology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008168:Lung / Q000294:innervation / Q000473:pathology; D009410:Nerve Degeneration / Q000473:pathology*; D012127:Respiratory Distress Syndrome, Newborn / Q000473:pathology*; D014132:Trachea / Q000294:innervation / Q000473:pathology