Toxoplasma gondii: effects of neuwiedase, a metalloproteinase from Bothrops neuwiedi snake venom, on the invasion and replication of human fibroblasts in vitro.
The major aim to the present study was to determine the effects of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi snake venom, on invasion and replication of Toxoplasma gondii in human fibroblasts in vitro. Neuwiedase treatment was done on host cells previously infected with T. gondii or on parasite before fibroblast infection. When treatments were done after or before infection, infection rates were inhibited in 71% and 61%, respectively. Considering that therapy protocols currently used in T. gondii infection cause considerable side effects, particularly in immunocompromised individuals and pregnant women, the results of neuwiedase treatment described herein could be taken into account for the development of new synthetic therapeutic agents, mainly due to the capacity of this enzyme to degrade extracellular matrix components, such as laminin, fibronectin and type I collagen, which is important to interfere in T. gondii host cell invasion.
Bastos
Luciana Machado
LM
Laboratory of Protein Chemistry and Natural Products, Institute of Genetic and Biochemistry, Federal University of Uberlândia, Av. Pará, 1720, 38400-902 Uberlândia, MG, Brazil.
Júnior
Robson J Oliveira
RJ
Silva
Deise Aparecida Oliveira
DA
Mineo
José Roberto
JR
Vieira
Carlos Ueira
CU
Teixeira
David Nascimento Silva
DN
Homsi-Brandeburgo
Maria Inês
MI
Rodrigues
Veridiana Melo
VM
Hamaguchi
Amélia
A
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000469:parasitology; D006801:Humans; D020128:Inhibitory Concentration 50; D016209:Interleukin-8 / Q000096:biosynthesis / Q000187:drug effects; D008297:Male; D008666:Metalloendopeptidases / Q000633:toxicity*; D051379:Mice; D014122:Toxoplasma / Q000187:drug effects* / Q000502:physiology; D014757:Viper Venoms / Q000633:toxicity*
D016209:Interleukin-8; D014757:Viper Venoms; D008666:Metalloendopeptidases; C414740:neuwiedase