[Associations of the genotypes of the CYP1A1 gene with predisposition to hydatid disease caused by Echinococcus granulosus strain G1].
DNA samples isolated from peripheral venous blood lymphocytes in 73 children with hydatid disease were studied. The polymorphism of exon 7 (A4889G) of the CYP1A1 gene was analyzed by polymerase chain reaction, followed by hydrolysis with restriction endonuclease HincII. The material for E. granulosus genotypes to be studied was obtained from the germinal layer of larvocysts. The fragment of the mitochondrial gene encoding for the first subunit of cytochome-C-oxidase was as a DNA marker. The amplified E. granulosus DNA fragments underwent direct sequencing and a genotype was identified. The findings have led to the conclusion that carriage of polymorphic allele Val of exon 7 (A4889G) of the CYP1A1 gene in those infested with E. granulosus genotype G1 (common, sheep strain) is a risk factor of the development of the clinical form of echinococcosis granulosus.
Lukmanova
G I
GI
Gumerov
A A
AA
Balalov
F S
FS
Komissarova
M A
MA
Lukmanova
L I
LI
Viktorova
T V
TV
D000293:Adolescent; D000483:Alleles; D000818:Animals; D002648:Child; D002675:Child, Preschool; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D004443:Echinococcosis / Q000235:genetics*; D048209:Echinococcus granulosus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005091:Exons / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D007963:Leukocytes, Mononuclear; D008297:Male; D012307:Risk Factors
D019363:Cytochrome P-450 CYP1A1