title: Collagen-binding motif peptide, a cleavage product of osteopontin, stimulates human neutrophil chemotaxis via pertussis toxin-sensitive G protein-mediated signaling.
abstract: The collagen-binding motif (CBM) peptide, a cleavage product of osteopontin (OPN), stimulated intracellular calcium increase in human neutrophils. CBM peptide-stimulated calcium was inhibited by pertussis toxin (PTX), suggesting the influence of PTX-sensitive G-proteins. In addition CBM peptide stimulated the chemotactic migration of human neutrophils and human monocytes. CBM peptide-induced neutrophil chemotaxis was completely inhibited by PTX, once again indicating the influence of Gi proteins. CBM peptide was also found to induce mitogen activated protein kinase activation. CBM peptide-induced neutrophil chemotaxis was mediated by p38 kinase as well as an extracellular signal-regulated protein kinase. Taken together, the results suggest that a cleavage product of OPN, CBM peptide, initiates immune responses by inducing neutrophil trafficking via certain PTX-sensitive cell surface receptors.
authors.affiliation: Department of Biochemistry, College of Medicine, Dong-A University, 3-1 Dongdaesindong Seogu, Busan 602-714, Republic of Korea.
authors.forename: Mi-Kyoung
authors.initials: MK
authors.lastname: Kim
authors.forename: Sang Doo
authors.initials: SD
authors.lastname: Kim
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Shim
authors.forename: Jeanho
authors.initials: J
authors.lastname: Yun
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Do Sik
authors.initials: do S
authors.lastname: Min
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Yoo
authors.forename: Yoe-Sik
authors.initials: YS
authors.lastname: Bae
mesh_terms: D020816:Amino Acid Motifs; D002478:Cells, Cultured; D002633:Chemotaxis; D003094:Collagen / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D053495:Osteopontin / Q000378:metabolism / Q000494:pharmacology*; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology*; D037342:Pertussis Toxin / Q000494:pharmacology
chemical_list: D010446:Peptide Fragments; D053495:Osteopontin; D003094:Collagen; D037342:Pertussis Toxin; D048049:Extracellular Signal-Regulated MAP Kinases; D019204:GTP-Binding Proteins