In vitro cell infiltration and in vivo cell infiltration and vascularization in a fibrous, highly porous poly(D,L-lactide) scaffold fabricated by cryogenic electrospinning technique.
One of the obstacles limiting the application of electrospun scaffolds for tissue engineering is the nanoscale pores that inhibit cell infiltration. In this article, we describe a technique that uses ice crystals as templates to fabricate cryogenic electrospun scaffolds (CES) with large three-dimensional and interconnected pores using poly(D,L-lactide) (PLA). Manipulating the humidity of the electrospinning environment the pore sizes are controlled. We are able to achieve pore sizes ranging from 900 +/- 100 microm(2) to 5000 +/- 2000 microm(2) depending on the relative humidity used. Our results show that cells infiltrated the CES up to 50 microm in thickness in vitro under static culture conditions whereas cells did not infiltrate the conventional electrospun scaffolds. In vivo studies demonstrated improved cell infiltration and vascularization in the CES compared with conventionally prepared electrospun scaffolds. In gaining control of the pore characteristics, we can then design CES that are optimized for specific tissue engineering applications.
Leong
Meng Fatt
MF
School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore.
Rasheed
Mohamed Zulfikar
MZ
Lim
Tze Chiun
TC
Chian
Kerm Sin
KS
D000818:Animals; D002452:Cell Count; D005347:Fibroblasts / Q000166:cytology*; D020007:Implants, Experimental; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D018919:Neovascularization, Physiologic; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D011091:Polyesters; C033616:poly(lactide)