The cyclin-dependent kinase Cdk1 directly regulates vacuole inheritance.
In budding yeast, vacuole inheritance is tightly coordinated with the cell cycle. The movement of vacuoles and several other organelles is actin-based and is mediated by interaction between the yeast myosin V motor Myo2 and organelle-specific adaptors. Myo2 binds to vacuoles via the adaptor protein Vac17, which binds to the vacuole membrane protein Vac8. Here we show that the yeast cyclin-dependent kinase Cdk1 phosphorylates Vac17 and that phosphorylation of Vac17 parallels cell cycle-dependent movement of the vacuole. Substitution of the Cdk1 sites in Vac17 decreases its interaction with Myo2 and causes a partial defect in vacuole inheritance. This defect is enhanced in the presence of Myo2 with mutated phosphorylation sites. Thus, Cdk1 appears to control the timing of vacuole movement. The presence of multiple predicted Cdk1 sites in other organelle-specific myosin V adaptors suggests that the inheritance of other cytoplasmic organelles may be regulated by a similar mechanism.
Peng
Yutian
Y
Department of Cell & Developmental Biology, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA.
Weisman
Lois S
LS
Department of Cell & Developmental Biology, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: lweisman@umich.edu.
D000595:Amino Acid Sequence; D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology*; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D024701:Myosin Type V / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
D008074:Lipoproteins; C077512:MYO2 protein, S cerevisiae; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D029701:Saccharomyces cerevisiae Proteins; C472499:VAC17 protein, S cerevisiae; C111373:VAC8 protein, S cerevisiae; D033921:Vesicular Transport Proteins; D016203:CDC2 Protein Kinase; D024701:Myosin Type V; D018995:Myosin Heavy Chains