A novel dynamic heterogeneous phase polymerization reaction for poly-hemoglobin with narrow molecular weight distribution.
A dynamic heterogeneous phase polymerization reaction is found to be efficient for controllable cross-link of hemoglobin with glutaraldehyde. The selective absorption of the immobile phase and asymmetry of protein concentration leads to narrowness of the molecular weight distribution and lowness of the average molecular weight. Using this method, 53% of hemoglobin obtained is intermolecular cross-linked with 12 molecular equivalents of glutaraldehyde. The majority of poly-hemoglobins is in the range of 128 kD to 258 kD.
Wang
Xiang
X
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China. tpuwx2008@gmail.com
Huang
Lei
L
Wang
Jin-Feng
JF
Yang
Cheng-Min
CM
D000042:Absorption; D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004912:Erythrocytes / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D010100:Oxygen / Q000378:metabolism
D001802:Blood Substitutes; D003432:Cross-Linking Reagents; D006454:Hemoglobins; C037158:polyhemoglobin; D010100:Oxygen; D005976:Glutaral