Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion.
We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.
Lue
Niyom
N
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Choi
Wonshik
W
Popescu
Gabriel
G
Badizadegan
Kamran
K
Dasari
Ramachandra R
RR
Feld
Michael S
MS
D000465:Algorithms; D000818:Animals; D003584:Cytological Techniques; D003952:Diagnostic Imaging / Q000379:methods; D005583:Fourier Analysis; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods; D015233:Models, Statistical; D009038:Motion; D055095:Optics and Photonics; D012031:Refractometry