Opisthorchis viverrini: detection by polymerase chain reaction (PCR) in human stool samples.
A polymerase chain reaction (PCR) assay was evaluated for detection of Opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in Khon Kaen province of Thailand. A total of 75 fecal specimens were analyzed by PCR following DNA extraction. All the microscopically positive samples were positive by PCR, while 23 of 30 (76.6%) microscopically negative samples were also PCR positive. The sensitivity of the assay was 5 eggs/g of stool. This method is potentially useful in the diagnosis of human opisthorchiasis in endemic areas for treatment and in epidemiological investigations.
Umesha
Kanasinakatte R
KR
Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Kankanady Post, Yekkur, Mangalore 575 002, India.
Kumar
Sanath
S
Parvathi
Ammini
A
Duenngai
Kunyarat
K
Sithithaworn
Paiboon
P
Karunasagar
Indrani
I
Karunasagar
Iddya
I
D000818:Animals; D018509:DNA, Helminth / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005260:Female; D006801:Humans; D008297:Male; D009889:Opisthorchiasis / Q000175:diagnosis* / Q000469:parasitology; D009891:Opisthorchis / Q000235:genetics / Q000302:isolation & purification*; D010063:Ovum; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D018509:DNA, Helminth