title: The generation and analysis of deficiencies within a small genomic region on the X chromosome of Drosophila melanogaster containing two genes, enhancer of rudimentary and CG15352.
abstract: The enhancer of rudimentary gene, e(r), encodes a 104-amino-acid, highly conserved transcription cofactor. Hypomorphic mutations of e(r) show an enhancement of a hypomorphic rudimentary mutant wing phenotype. These mutants in a wild-type background are viable, fertile, and morphologically wild-type. Since the only mutant alleles were hypomorphic, it was important to isolate null mutations to determine if any other phenotypes might be associated with a loss-of-function of e(r). We utilized a marked P element, P{SUPor-P, y(+)}, located 895 bp upstream of the start of transcription of e(r) to generate nineteen deficiencies in the region. Deficiencies of e(r) enhance the mutant wing phenotype of a hypomorphic rudimentary allele, r(hd1). In a wild-type background, the deficiencies of e(r), unlike the hypomorphic alleles, have a low viability and females have low fertility. The expression of e(r) in the nurse cells of the ovary is consistent with the low fertility, and suggests an ovarian function for e(r). Deficiencies of CG15352, the gene directly upstream of e(r), are not associated with any obvious mutant phenotypes and present the possibility that it encodes a nonvital or redundant function.
authors.affiliation: Department of Biology, Saint Louis University, St Louis, Missouri, USA.
authors.forename: Margarita
authors.initials: M
authors.lastname: Roykhman
authors.forename: Suzanne
authors.initials: S
authors.lastname: Ibach
authors.forename: Deloris
authors.initials: D
authors.lastname: Harding
authors.forename: Brian
authors.initials: B
authors.lastname: Wolff
authors.forename: Melissa
authors.initials: M
authors.lastname: Vowels
authors.forename: Mark E
authors.initials: ME
authors.lastname: Gelsthorpe
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Wojcik
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: Rizzo
authors.forename: Stuart I
authors.initials: SI
authors.lastname: Tsubota
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D019852:Diacylglycerol Kinase; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014921:Wings, Animal / Q000254:growth & development; D014960:X Chromosome
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C105076:e(r) protein, Drosophila; D019852:Diacylglycerol Kinase; C511350:RdgA protein, Drosophila