Development of an immunochromatographic assay for rapid detection of 1-Aminohydantoin in urine specimens.
A rapid immunochromatographic assay was developed and validated for detection of 1-aminohydantoin (AHD) in urine specimens. Colloidal gold-labeled polyclonal antibody specific to AHD derivative was used as the marker; based on the competitive reactivity theory, the metabolite of nitrofurantoin after derivatization with benzaldehyde would compete with carboxyphenyl AHD derivative-conjugated ovalbumin. The test strip could efficaciously detect the novel analyte with a visual detection limit of 10 ng mL(-1) and high specificity. The reliability of the assay was determined by testing 80 standard samples comparing with enzyme-linked immunosorbent assay. The semi-quantitative detection was accomplished in less than 15 min with low cost, especially for requirements of rapid and simple screening. This is the first publication of an immunochromatographic assay for detection of nitrofuran residues.
Xu
Yi Ping
YP
Jiangnan University, Wuxi, Jiangsu, People's Republic of China.
Liu
Li Qiang
LQ
Li
Qiu Shen
QS
Peng
Chi Fang
CF
Chen
Wei
W
Xu
Chuan Lai
CL
D000906:Antibodies / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D017979:Gold Colloid / Q000737:chemistry; D006801:Humans; D006827:Hydantoins / Q000378:metabolism / Q000652:urine*; D007118:Immunoassay / Q000379:methods*; D008956:Models, Chemical; D009581:Nitrofurans / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
C504350:1-aminohydantoin; D000906:Antibodies; D017979:Gold Colloid; D006827:Hydantoins; D009581:Nitrofurans