[Optimization of bacterial cellulose fermentation medium and observation of bacterial cellulose ultra-micro-structure].
In order to improve the yield of bacterial cellulose (BC), the fermentation medium of BC-producing strain J2 (Gluconobacter) was optimized, and BC ultra-micro-structure was observed. Initially, Plackett-Burman design was employed to evaluate eight variables which were relevant to BC production. Three statistically significant parameters including yeast extract, ZnSO4, ethanol were selected and other 5 variables were not significant (P > 0.05). The optimized levels of three variables were defined by Box-Behnken design and response surface methodology (RSM). BC ultra-micro-structure was observed by scanning electron microscope (SEM) with cotton cellulose as comparison. The results indicated that the BC yield under the optimum fermentation medium was 11.52 g/100 mL, which was as 1.35 times as that under the original fermentation medium. The SEM photos manifested that bacterial cellulose ribbon, with a diameter less than 0.1 microm, was less than cotton cellulose ribbon. The bacteria inside the cellulose net were eliminated after the NaOH treatment.
Wu
Ruiqin
R
College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China.
Du
Shuangkui
S
Li
Zhixi
Z
Xing
Xiaohui
X
Shao
Dongyan
D
Fan
Yanli
Y
Li
Bo
B
Zhang
Xueliang
X
Bu
Lijun
L
D018929:Cell Culture Techniques; D002482:Cellulose / Q000096:biosynthesis* / Q000648:ultrastructure*; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D020576:Gluconobacter / Q000166:cytology / Q000378:metabolism*
D003470:Culture Media; D002482:Cellulose