title: [Matrine inhibits the proliferation of prostate cancer cells and the activity of androgen receptor].
abstract: OBJECTIVE
  To study the inhibitory effect of matrine on the proliferation of the prostate cancer cell line LNCaP and the expression of the androgen receptor (AR).
  
  
  METHODS
  LNCaP cells were treated with matrine at the concentration of 0.5, 1.0, 1.5, 2.0 and 3.0 g/L for 12, 24 and 36 hours, the cell growth activity determined by MTT colorimetry and trypan blue staining at 36 hours, the cell cycle changes detected by flow cytometry and the expression of AR by Western blot at 24 hours.
  
  
  RESULTS
  Matrine suppressed the in vitro growth of the androgen-sensitive prostate cancer cell line LNCaP in a time- and dose-dependent manner, blocked the cell cycles in the G2/M phase and decreased the expression of AR in the cell line in a dose-dependent manner (P < 0.01).
  
  
  CONCLUSIONS
  Matrine can significantly inhibit the in vitro growth of NCaP cells by down-regulating the expression of AR and blocking cell cycles.
authors.affiliation: Department of Urology, Tongji Hospital, Wuhan, Hubei 430030, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.forename: Zhi-Quan
authors.initials: ZQ
authors.lastname: Hu
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Guo
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Zhang-Qun
authors.initials: ZQ
authors.lastname: Ye
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D011807:Quinolizines / Q000494:pharmacology*; D011944:Receptors, Androgen / Q000378:metabolism*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D011807:Quinolizines; D011944:Receptors, Androgen; D000093842:Matrines