title: Collagen and myosin characterization by orientation field second harmonic microscopy.
abstract: Collagen and myosin fibrils are endogenous harmonophores that both give rise to Second Harmonic Generation (SHG). By combining four polarization SHG images provided by a scanning microscope, we show that the orientation of the principal axis of the nonlinear susceptibility tensor chi(2) can be determined for each pixel of the image. The ratio rho = chi33/chi15 of the principal components of chi(2) of collagen and myosin was obtained with the same method, and found within the range 1.6-1.8 and 0.5-0.6 respectively. The orientation of the principal axis of chi(2) is shown to be correlated to the orientation of the fibrils themselves. This provides a straightforward method, which we call Orientation Field-Second Harmonic Microscopy (OF-SHM), to reconstruct orientation fields of fibrils at various scales and resolutions in different biological systems (from muscle sarcomere to the whole embryo).
authors.affiliation: Institut of Physics of Rennes IPR/UMR CNRS 6251, University of Rennes I, Campus deBeaulieu, Bat 11A, 35042 Rennes Cedex, France. christophe.odin@univ-rennes1.fr
authors.forename: Christophe
authors.initials: C
authors.lastname: Odin
authors.forename: Thomas
authors.initials: T
authors.lastname: Guilbert
authors.forename: Alia
authors.initials: A
authors.lastname: Alkilani
authors.forename: Olena P
authors.initials: OP
authors.lastname: Boryskina
authors.forename: Vincent
authors.initials: V
authors.lastname: Fleury
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Grand
mesh_terms: D000465:Algorithms; D000818:Animals; D002645:Chickens; D003094:Collagen / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008853:Microscopy / Q000379:methods*; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods; D009218:Myosins / Q000737:chemistry*; D025341:Principal Component Analysis; D012518:Sarcomeres / Q000378:metabolism; D012756:Sheep; D013710:Tendons / Q000473:pathology
chemical_list: D003094:Collagen; D009218:Myosins