title: Callogenic studies of Achyranthes aspera leaf explant at different hormonal combinations.
abstract: With the objective to promote in vitro callus induction, leaf segments of Achyranthes aspera were inoculated on basal MS medium supplemented with 3.0% sucrose and 0.8% agar with different concentrations of 2,4-D alone and in combination with NAA, BAP, IAA, IBA and Zeatin. The explants were maintained in growth room at 25 +/- 1 degrees C and 16 h light cycle. The best callus induction was obtained with 2,4-D (1.0 and 2.0 mg L(-l)) in combination with NAA (0.5 mg L(-1)). Callus induction and good texture from leaf explant was also observed at 2,4-D with BAP. On these combinations morphologically, light green, soft, compact and non-embryogenic callus (Type III callus) was observed. While morphology of callus and callogenic response was poor at 2,4-D alone or in combination with other hormones at different concentrations.
authors.affiliation: Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Sara
authors.initials: S
authors.lastname: Kayani
authors.forename: Muhammad
authors.initials: M
authors.lastname: Zia
authors.forename: Sadia
authors.initials: S
authors.lastname: Sarwar
authors.lastname: Riaz-ur-Rehman
authors.forename: M Fayyaz
authors.initials: MF
authors.lastname: Chaudhary
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D029044:Achyranthes / Q000378:metabolism*; D000362:Agar / Q000737:chemistry; D006728:Hormones / Q000737:chemistry*; D010936:Plant Extracts / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D013395:Sucrose / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D046509:Tissue Culture Techniques; D015026:Zeatin / Q000737:chemistry
chemical_list: D006728:Hormones; D010936:Plant Extracts; D015084:2,4-Dichlorophenoxyacetic Acid; D013395:Sucrose; D015026:Zeatin; D000362:Agar