title: Expression and localization of the spermatogenesis-related gene, Znf230, in mouse testis and spermatozoa during postnatal development.
abstract: Znf230, the mouse homologue of the human spermatogenesis-related gene, ZNF230, has been cloned by rapid amplification of cDNA ends (RACE). This gene is expressed predominantly in testis, but its expression in different testicular cells and spermatogenic stages has not been previously analyzed in detail. In the present study, the cellular localization of the Znf230 protein in mouse testis and epididymal spermatozoa was determined by RT-PCR, immunoblotting, immunohistochemistry and immunofluorescence. It is primarily expressed in the nuclei of spermatogonia and subsequently in the acrosome system and the entire tail of developing spermatids and spermatozoa. The results indicate that Znf230 may play an important role in mouse spermatogenesis, including spermatogenic cell proliferation and sperm maturation, as well as motility and fertilization.
authors.affiliation: Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University, Chengdu, PR China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Song
authors.forename: Dan
authors.initials: D
authors.lastname: Su
authors.forename: Pan
authors.initials: P
authors.lastname: Lu
authors.forename: Jiyun
authors.initials: J
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
authors.forename: Yunqiang
authors.initials: Y
authors.lastname: Liu
authors.forename: Sizhong
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000502:physiology*; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000906:Antibodies; D004268:DNA-Binding Proteins; C486693:Rnf141 protein, mouse; D014157:Transcription Factors