title: Tracking the structural dynamics of proteins in solution using time-resolved wide-angle X-ray scattering.
abstract: We demonstrate tracking of protein structural changes with time-resolved wide-angle X-ray scattering (TR-WAXS) with nanosecond time resolution. We investigated the tertiary and quaternary conformational changes of human hemoglobin under nearly physiological conditions triggered by laser-induced ligand photolysis. We also report data on optically induced tertiary relaxations of myoglobin and refolding of cytochrome c to illustrate the wide applicability of the technique. By providing insights into the structural dynamics of proteins functioning in their natural environment, TR-WAXS complements and extends results obtained with time-resolved optical spectroscopy and X-ray crystallography.
authors.affiliation: European Synchrotron Radiation Facility, Grenoble Cedex 38043, BP 220, France. marco.cammarata@esrf.eu
authors.forename: Marco
authors.initials: M
authors.lastname: Cammarata
authors.forename: Matteo
authors.initials: M
authors.lastname: Levantino
authors.forename: Friedrich
authors.initials: F
authors.lastname: Schotte
authors.forename: Philip A
authors.initials: PA
authors.lastname: Anfinrud
authors.forename: Friederike
authors.initials: F
authors.lastname: Ewald
authors.forename: Jungkweon
authors.initials: J
authors.lastname: Choi
authors.forename: Antonio
authors.initials: A
authors.lastname: Cupane
authors.forename: Michael
authors.initials: M
authors.lastname: Wulff
authors.forename: Hyotcherl
authors.initials: H
authors.lastname: Ihee
mesh_terms: D018360:Crystallography, X-Ray; D045304:Cytochromes c / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D011487:Protein Conformation; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D014965:X-Rays
chemical_list: D006454:Hemoglobins; D009211:Myoglobin; D045304:Cytochromes c