Affinity chromatography of phosphorylated proteins.
This chapter covers the use of immobilized metal ion affinity chromatography (IMAC) for enrichment of phosphorylated proteins. Some requirements for successful enrichment of these types of proteins are discussed. An experimental protocol and a set of application data are included to enable the scientist to obtain high-yield results in a very short time with pre-packed phospho-specific metal ion affinity resin (PMAC).
Tchaga
Grigoriy S
GS
Clontech Laboratories, Inc., Mountain View, CA, USA.
D015153:Blotting, Western; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry*
D011506:Proteins