Dissection of 6.5 dpc mouse embryos.
Analysis of gene expression patterns during early stages of mammalian embryonic development can provide important clues about gene function, cell-cell interaction and signaling mechanisms that guide embryonic patterning. However, dissection of the mouse embryo from the decidua shortly after implantation can be a challenging procedure, and detailed step-by-step documentation of this process is lacking. Here we demonstrate how post-implantation (6.5 dpc) embryos are isolated by first dissecting the uterus of a pregnant mouse (detection of the vaginal plug was designated day 0.5 poist coitum) and subsequently dissecting the embryo from maternal decidua. The dissection of Reichert's membrane is described as well as the removal of the ectoplacental cone.
Shea
Kelly
K
Massachusetts General Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA, USA.
Geijsen
Niels
N
D000818:Animals; D004210:Dissection / Q000379:methods*; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000601:surgery*; D005260:Female; D051379:Mice; D011247:Pregnancy