title: Molecular characterization of VP6-encoding gene of group A human rotavirus samples from central west region of Brazil.
abstract: Group A rotaviruses are the main cause of acute gastroenteritis in children worldwide. The intermediate capsid protein VP6 encoded by segment 6 of the dsRNA genome is the major structural component of the virus and it is highly antigenic and immunogenic. VP6 is responsible for group and subgroup (SG) specificities, allowing classification of group A rotavirus into SG I, SG II, SG I + II, and SG non-I-non-II. VP6-encoding gene of 154 group A human rotavirus samples of different G and P genotypes recovered from children in three cities of Central West region of Brazil was amplified by reverse transcription-polymerase chain reaction followed by sequencing and phylogenetic analysis. Two distinct genetic groups could be recognized: VP6 genogroups I and II. Sequences analysis also revealed that all samples identified as VP6 genogroup I were associated with NSP4 genotype A, whereas samples identified as VP6 genogroup II were associated with NSP4 genotype B. This is the first study in Central West region regarding genetic variability of the VP6 gene. Further molecular surveillance of rotavirus strains is needed to understand better the occurrence of VP6 gene diversity in Brazil and the significance of VP6 for the control and prevention of rotavirus gastroenteritis.
authors.affiliation: Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Brazil.
authors.forename: Talissa de Moraes
authors.initials: Tde M
authors.lastname: Tavares
authors.forename: Wilia Marta Elsner Diederichsen
authors.initials: WM
authors.lastname: de Brito
authors.forename: Fabíola Souza
authors.initials: FS
authors.lastname: Fiaccadori
authors.forename: Juliana Alves
authors.initials: JA
authors.lastname: Parente
authors.forename: Paulo Sérgio Sucasas
authors.initials: PS
authors.lastname: da Costa
authors.forename: Loreny Gimenes
authors.initials: LG
authors.lastname: Giugliano
authors.forename: Márcia Sueli Assis
authors.initials: MS
authors.lastname: Andreasi
authors.forename: Célia Maria Almeida
authors.initials: CM
authors.lastname: Soares
authors.forename: Divina das Dôres de Paula
authors.initials: Dd
authors.lastname: Cardoso
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics*; D001938:Brazil / Q000453:epidemiology; D036022:Capsid Proteins / Q000235:genetics*; D002675:Child, Preschool; D016000:Cluster Analysis; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C061332:VP6 protein, Rotavirus