Comparative study of the pln locus of the quorum-sensing regulated bacteriocin-producing L. plantarum J51 strain.
Lactobacillus plantarum J51 strain was isolated from a Rioja red wine and it showed bacteriocin activity against a wide range of lactic acid bacteria of oenological importance. These characteristics conferred L. plantarum J51 a high interest both in wine microbiology and in the study of bacteriocin production. In this work the bacteriocin production regulated under the "quorum-sensing" mechanism is observed and the pln locus of the bacteriocin-producing L. plantarum J51 is fully characterized. A 20,667 bp fragment was completely sequenced (GenBank accession number DQ340868), and showed five operons (plNC8betaalphac, plnLR-like, plnABCD, plnEFI, plnGHSTUVW) and a new region containing a putative operon with three new orfs that could encode a putative two-peptide bacteriocin.
Navarro
Laura
L
Area de Bioquímica y Biología Molecular, Departamento de Agricultura y Alimentación, Universidad de La Rioja, Logroño, Spain.
Rojo-Bezares
Beatriz
B
Sáenz
Yolanda
Y
Díez
Lorena
L
Zarazaga
Myriam
M
Ruiz-Larrea
Fernanda
F
Torres
Carmen
C
D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D053938:DNA Fragmentation; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D005516:Food Microbiology; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009876:Operon; D011498:Protein Precursors; D053038:Quorum Sensing / Q000502:physiology*; D016415:Sequence Alignment; D013045:Species Specificity; D014920:Wine / Q000382:microbiology*
D001430:Bacteriocins; D004269:DNA, Bacterial; D011498:Protein Precursors; C407314:plnA protein, Lactobacillus plantarum