Biodegradation of insecticide carbofuran by Paracoccus sp. YM3.
A bacterium (Paracoccus sp. YM3) capable of degrading carbofuran was isolated from carbofuran-contaminated sludge. The strain was shown to metabolize carbofuran (50 mg L(-1)) to carbofuran-7-phenol in minimal salt medium within 6 days in which the pesticide was the only source of carbon. Carbofuran and its main metabolite were analyzed by high performance liquid chromatography (HPLC). The addition of an other carbon source led to accelerated biodegradation. The relevant degrading-enzyme was intracellular and inducible. A tobacco hypersensitivity experiment showed that YM3 could eliminate carbofuran in soils effectively and safely. This is the first report of a Paracoccus sp. that could degrade carbofuran. The present study may provide a basis for biotreatment of wastewaters and bioremediation of carbofuran-contaminated soils.
Peng
Xiang
X
College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, PR China.
Zhang
Jing S
JS
Li
Ying Y
YY
Li
Wen
W
Xu
Gang M
GM
Yan
Yan C
YC
D001426:Bacterial Proteins / Q000235:genetics; D001673:Biodegradation, Environmental / Q000187:drug effects; D002235:Carbofuran / Q000737:chemistry*; D005786:Gene Expression Regulation; D007306:Insecticides / Q000737:chemistry*; D042541:Intracellular Space / Q000201:enzymology; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000378:metabolism; D010230:Paracoccus / Q000145:classification / Q000378:metabolism* / Q000472:pathogenicity / Q000502:physiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000032:analysis; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D014026:Nicotiana / Q000382:microbiology
D001426:Bacterial Proteins; D007306:Insecticides; D009930:Organic Chemicals; D012336:RNA, Ribosomal, 16S; D012987:Soil; D013395:Sucrose; D002235:Carbofuran