IFATS collection: Immunomodulatory effects of adipose tissue-derived stem cells in an allergic rhinitis mouse model.
Adipose tissue-derived stem cells (ASCs) exhibit immunosuppressive effects in allogeneic transplantation. However, there is no report that evaluates the in vivo immune-modulating effect of ASCs in an experimental allergic rhinitis (AR) model. We investigated whether ASCs migrate to the nasal mucosa in an AR mouse model and evaluated the immune-modulating effect of ASCs in the AR mouse model. Cultured ASCs (2 x 10(6)) were injected i.v. before the first allergen challenge in the AR mouse model. Migration of ASCs to the nasal mucosa was evaluated by immunofluorescence. The immunomodulatory effects of ASCs were evaluated by nasal symptoms, histology, serum ovalbumin (OVA)-specific antibody, and the cytokine profile of the spleen. ASCs migrated to the nasal mucosa in the AR mouse model. ASCs significantly reduced allergic symptoms and inhibited eosinophilic inflammation in the nasal mucosa. ASCs significantly decreased the serum allergen-specific IgE level and the IgG(1)/IgG(2a) ratio and significantly increased the IgG(2a) level in the AR mouse model. ASCs inhibited interleukin (IL)-4 and IL-5 production from OVA-incubated splenocytes, but enhanced interferon-gamma production. In conclusion, ASCs can migrate to the nasal mucosa in the AR mouse model and inhibit eosinophilic inflammation partly via shifting to a T-helper 1 (Th1) from a Th2 immune response to allergens.
Cho
Kyu-Sup
KS
Department of Otorhinolaryngology, Medical Research Institute, Pusan National University School of Medicine, Busan, Korea.
Park
Hye-Kyung
HK
Park
Hee-Young
HY
Jung
Jin Sup
JS
Jeon
Seong-Gyu
SG
Kim
Yoon-Keun
YK
Roh
Hwan Jung
HJ
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D000917:Antibody Formation; D002452:Cell Count; D002454:Cell Differentiation; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D000939:Epitopes; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007155:Immunologic Factors / Q000276:immunology*; D016130:Immunophenotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000276:immunology / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D012221:Rhinitis, Allergic, Perennial / Q000097:blood / Q000276:immunology*; D013154:Spleen / Q000378:metabolism; D013234:Stem Cells / Q000276:immunology*
D016207:Cytokines; D000939:Epitopes; D007074:Immunoglobulin G; D007155:Immunologic Factors; D007073:Immunoglobulin E; D010047:Ovalbumin