Cellulose degradation by micromonosporas recovered from freshwater lakes and classification of these actinomycetes by DNA gyrase B gene sequencing.
A number of Micromonospora strains isolated from the water column, sediment, and cellulose baits placed in freshwater lakes were shown to be able to degrade cellulose in lake water without any addition of nutrients. A selective isolation method was also developed to demonstrate that CFU arose from both spores and hyphae that inhabit the lake environment. Gyrase B gene sequencing performed on the isolates identified a number of new centers of variation within Micromonospora, but the most actively cellulolytic strains were recovered in a single cluster that equated with the type species of the genus, M. chalcea.
de Menezes
Alexandre B
AB
School of Biological Sciences, Biosciences Building, University of Liverpool, Crown Street, Liverpool, Merseyside L69 7ZB, United Kingdom.
Lockhart
Robert J
RJ
Cox
Michael J
MJ
Allison
Heather E
HE
McCarthy
Alan J
AJ
D002482:Cellulose / Q000378:metabolism*; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D002482:Cellulose; D027081:DNA Gyrase