title: Rapid biofabrication of tubular tissue constructs by centrifugal casting in a decellularized natural scaffold with laser-machined micropores.
abstract: Centrifugal casting allows rapid biofabrication of tubular tissue constructs by suspending living cells in an in situ cross-linkable hydrogel. We hypothesize that introduction of laser-machined micropores into a decellularized natural scaffold will facilitate cell seeding by centrifugal casting and increase hydrogel retention, without compromising the biomechanical properties of the scaffold. Micropores with diameters of 50, 100, and 200 mum were machined at different linear densities in decellularized small intestine submucosa (SIS) planar sheets and tubular SIS scaffolds using an argon laser. The ultimate stress and ultimate strain values for SIS sheets with laser-machined micropores with diameter 50 mum and distance between holes as low as 714 mum were not significantly different from unmachined control SIS specimens. Centrifugal casting of GFP-labeled cells suspended in an in situ cross-linkable hyaluronan-based hydrogel resulted in scaffold recellularization with a high density of viable cells inside the laser-machined micropores. Perfusion tests demonstrated the retention of the cells encapsulated within the HA hydrogel in the microholes. Thus, an SIS scaffold with appropriately sized microholes can be loaded with hydrogel encapsulated cells by centrifugal casting to give a mechanically robust construct that retains the cell-seeded hydrogel, permitting rapid biofabrication of tubular tissue construct in a "bioreactor-free" fashion.
authors.affiliation: Medical University of South Carolina, Charleston, SC 29426, USA.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Kasyanov
authors.forename: Jason
authors.initials: J
authors.lastname: Hodde
authors.forename: Michael C
authors.initials: MC
authors.lastname: Hiles
authors.forename: Carol
authors.initials: C
authors.lastname: Eisenberg
authors.forename: Leonard
authors.initials: L
authors.lastname: Eisenberg
authors.forename: Luis E F
authors.initials: LE
authors.lastname: De Castro
authors.forename: Iveta
authors.initials: I
authors.lastname: Ozolanta
authors.forename: Modra
authors.initials: M
authors.lastname: Murovska
authors.forename: Robert A
authors.initials: RA
authors.lastname: Draughn
authors.forename: Glenn D
authors.initials: GD
authors.lastname: Prestwich
authors.forename: Roger R
authors.initials: RR
authors.lastname: Markwald
authors.forename: Vladimir
authors.initials: V
authors.lastname: Mironov
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D002460:Cell Line; D002470:Cell Survival; D006801:Humans; D020100:Hydrogels; D007413:Intestinal Mucosa / Q000098:blood supply / Q000737:chemistry; D007834:Lasers; D008422:Materials Testing; D008648:Mesoderm / Q000166:cytology; D011784:Quail; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020100:Hydrogels