Simplified validation of borderline hits of database searches.
Along with unequivocal hits produced by matching multiple MS/MS spectra to database sequences, LC-MS/MS analysis often yields a large number of hits of borderline statistical confidence. To simplify their validation, we propose to use rapid de novo interpretation of all acquired MS/MS spectra and, with the help of a simple software tool, display the candidate sequences together with each database search hit. We demonstrate that comparing hit database sequences and independent de novo interpretations of the same MS/MS spectra assists in rapid examination of ambiguous matches.
Thomas
Henrik
H
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Shevchenko
Andrej
A
D030541:Databases, Genetic; D030562:Databases, Protein; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods; D020539:Sequence Analysis, Protein; D012984:Software; D053719:Tandem Mass Spectrometry / Q000379:methods*; D054928:Validation Studies as Topic