title: Calcium ion cyclotron resonance (ICR) transfers information to living systems: effects on human epithelial cell differentiation.
abstract: The specific aim of the present work concerns the effectiveness of low-frequency electromagnetic fields treatment to modify biochemical properties of human keratinocytes (HaCaT). Cells exposed to a 7 Hz electromagnetic field, tuned to calcium ion cyclotron resonance (ICR), showed modifications in the cytoskeleton. These modifications were related to different actin distributions as revealed by phalloidin fluorescence analysis. Indirect immunofluorescence with fluorescent antibodies against involucrin and beta catenin, both differentiation and adhesion markers, revealed an increase in involucrin and beta-catenin expression, indicating that exposure to electromagnetic field carries keratinocytes to an upper differentiation level. This study confirms our previous observation and supports the hypothesis that a 7 Hz calcium ICR electromagnetic field may modify cell biochemistry and interfere in the differentiation and cellular adhesion of normal keratinocytes, suggesting the possibility to use ICR electromagnetic therapy for the treatment of undifferentiated diseases.
authors.affiliation: Istituto di Neurobiologia e Medicina Molecolare CNR, Rome, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Lisi
authors.forename: Mario
authors.initials: M
authors.lastname: Ledda
authors.forename: Flavia
authors.initials: F
authors.lastname: De Carlo
authors.forename: Alberto
authors.initials: A
authors.lastname: Foletti
authors.forename: Livio
authors.initials: L
authors.lastname: Giuliani
authors.forename: Enrico
authors.initials: E
authors.lastname: D'Emilia
authors.forename: Settimio
authors.initials: S
authors.lastname: Grimaldi
mesh_terms: D000199:Actins / Q000378:metabolism; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation / Q000528:radiation effects*; D017357:Cyclotrons; D004599:Electric Stimulation Therapy; D004574:Electromagnetic Fields; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D011498:Protein Precursors / Q000378:metabolism; D015203:Reproducibility of Results; D051176:beta Catenin / Q000378:metabolism
chemical_list: D000199:Actins; D011498:Protein Precursors; D051176:beta Catenin; C032253:involucrin; D002118:Calcium