[Dysregulation of cooperative interactions of immunocytes and eosinophils in the mechanism of development of eosinophilia in Opisthorhis felineus invasion].
The authors studied the levels of mononuclear cell production of eosinophil-specific cytokines (IL-3, IL-5), the serum levels of eotaxin by enzyme immunoassay; the expression of the eosinophilic cell receptor apparatus by flow cytofluorometry in patients with acute and chronic Opisthorchis invasion. Eosinophilia-associated Opisthorchis invasion was found to be accompanied by a pronounced change in the serum production of the key cytokine regulators of eosinophilic homeostasis (elevated IL-3 and IL-5 levels) and eotaxin by peripheral blood mononuclear leukocytes. There was an increase in the number of receptor structures to eosinophil-specific cytokines (IL-5R-, IL-3R-, and CCR3-positive cells) in patients with opisthorchiasis. In vitro incubation of the eosinophils, obtained from patients with opisthorchiasis, with the recombinant forms of cytokines (IL-5, IL-3, and eotaxin) demonstrated the decreased expression of IL-5 and IL-3 receptors with the normal presentation of CCR3. With the developed acute helminthiasis, the revealed changes were more pronounced than those observed in chronic Opisthorchis invasion.
Litvinova
L S
LS
Riazantseva
N V
NV
Novitskiĭ
V V
VV
D000293:Adolescent; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D019742:Chemokines, CC / Q000097:blood / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D004802:Eosinophilia / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007377:Interleukin-3 / Q000096:biosynthesis / Q000097:blood / Q000494:pharmacology; D015848:Interleukin-5 / Q000096:biosynthesis / Q000097:blood / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D009889:Opisthorchiasis / Q000097:blood / Q000150:complications / Q000276:immunology*; D009891:Opisthorchis; D054397:Receptors, CCR3 / Q000276:immunology; D016185:Receptors, Interleukin-3 / Q000378:metabolism; D053649:Receptors, Interleukin-5 / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
D019742:Chemokines, CC; D007377:Interleukin-3; D015848:Interleukin-5; D054397:Receptors, CCR3; D016185:Receptors, Interleukin-3; D053649:Receptors, Interleukin-5; D011994:Recombinant Proteins