title: [Investigation of viral nucleic acids in middle-ear effusion specimens from children with acute otitis media].
abstract: Acute otitis media with effusion (OME) is one of the major causes of antibiotic use, indication for operation and hearing loss in children. In two third of the cases the etiologic agents are bacteria. Nonetheless, increasing numbers of reports have implicated viruses as etiologic agents that may have some effect on prognosis of OME. The aim of this study was to investigate the presence of nucleic acids of respiratory syncytial virus (RSV) type A and B, influenza type A virus, adenovirus, cytomegalovirus (CMV), herpes simplex virus type-1 (HSV-1), and enteroviruses in the middle ear effusion specimens from children with otitis media by TaqMan real-time PCR. As a result, 18 of 30 (60%) OME samples were found positive in terms of viral nucleic acids by real-time PCR. RSV-A was detected in nine samples (30%), CMV in 3 (10%) samples and HSV-1 in 1 (3.3%) sample. In five of the samples two viruses were detected in the same sample (three were positive for adenovirus and RSV-A, and two were positive for CMV and RSV-A). Our data have supported the importance of viruses as etiologic agents of OME. Additionally, it was thought that TaqMan real-time PCR may be used as a reliable and rapid method for the detection of viruses in the middle ear effusion samples.
authors.affiliation: Kral Hiseyin Tip Merkezi, Urdün.
authors.forename: Muhammed H
authors.initials: MH
authors.lastname: Abu Sitteh
authors.forename: Kenan
authors.initials: K
authors.lastname: Sener
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yapar
authors.forename: Abdullah
authors.initials: A
authors.lastname: Kiliç
authors.forename: Cakir
authors.initials: C
authors.lastname: Güney
authors.forename: Ayhan
authors.initials: A
authors.lastname: Kubar
mesh_terms: D000208:Acute Disease; D000260:Adenoviruses, Human / Q000235:genetics / Q000302:isolation & purification; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D010034:Otitis Media with Effusion / Q000821:virology*; D011379:Prognosis; D012367:RNA, Viral / Q000302:isolation & purification*; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral