Convergent and general one-step DNA-catalyzed synthesis of multiply branched DNA.
We report a deoxyribozyme (DNA enzyme) that catalyzes the convergent and general synthesis of branched DNA. The 15HA9 deoxyribozyme mediates nucleophilic attack of the 2'-hydroxyl group of a ribonucleotide embedded within one DNA substrate into a 5'-adenylate of the second DNA substrate. This approach can be used to synthesize multiply branched DNA with a wide range of DNA sequences.
Mui
Timothy P
TP
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
Silverman
Scott K
SK
D001483:Base Sequence; D002384:Catalysis; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D021881:DNA, Catalytic / Q000378:metabolism
D021881:DNA, Catalytic; D004247:DNA