Genetic, genomic and physiological state studies on single-needle bio-electrosprayed human cells.
Bio-electrospraying, a non-contact jet-based direct cell engineering approach, was recently pioneered and demonstrated for handling a wide range of primary living cells. In those studies, post-treated cells were biologically assessed in comparison to several controls by way of flow cytometry. Although flow cytometry accurately assesses those viable populations of cells, subtle effects at a sub-cellular level could have been missed. Therefore, in the present study we demonstrate metaphase chromosome breakage studies carried out on single-needle bio-electrosprayed human T-lymphocytes, which are compared with several controls. The results indicate that post-treated T-lymphocytes do not demonstrate any increase in chromosome damage in comparison to control cells. These studies further validate bio-electrospraying as a technique with potential for clinical utility.
Hall
Richard P
RP
Cytogenetics Department, Guy's Hospital, 5th Floor Tower Wing, Great Maze Pond, London, UKSE1 9RT.
Ogilvie
Caroline M
CM
Aarons
Emma
E
Jayasinghe
Suwan N
SN
D002452:Cell Count; D002470:Cell Survival; D019457:Chromosome Breakage; D005434:Flow Cytometry; D006801:Humans; D008677:Metaphase; D013601:T-Lymphocytes / Q000648:ultrastructure*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*