title: Assessment of diagnostic enzyme-linked immunosorbent assay kit and serological markers in human brucellosis.
abstract: This study was performed to evaluate commercial brucella immunoglobulin G and M enzyme-linked immunosorbent assay (IgG and IgM ELISA) kits for the diagnosis of human brucellosis and to suggest a candidate prognostic marker for human brucellosis. We determined the serum levels of brucella IgG, IgM, C-reactive protein (CRP), soluble CD14 (sCD14), and neopterin in patients with brucellosis and compared them with those of normal healthy persons, patients with tuberculosis, and patients with other diseases. It was found that the sensitivity of ELISA to diagnose brucellosis was high when both IgG and IgM ELISA were used together. This study showed that serum CRP, sCD14, or neopterin levels were significantly high during the course of human brucellosis. The above markers, alone or in combination, might have the potential to evaluate treatment outcomes in human brucellosis. The markers that can predict the variability of agglutination titer was also determined. It was found that the titer value alone does not fully represent disease status.
authors.affiliation: Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.
authors.forename: Gulcin
authors.initials: G
authors.lastname: Cakan
authors.forename: Fatma Betul
authors.initials: FB
authors.lastname: Bezirci
authors.forename: Askin
authors.initials: A
authors.lastname: Kacka
authors.forename: Salih
authors.initials: S
authors.lastname: Cesur
authors.forename: Sebahat
authors.initials: S
authors.lastname: Aksaray
authors.forename: Deniz
authors.initials: D
authors.lastname: Tezeren
authors.forename: Dilek
authors.initials: D
authors.lastname: Saka
authors.forename: Kamruddin
authors.initials: K
authors.lastname: Ahmed
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D002097:C-Reactive Protein / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007162:Immunoproteins / Q000032:analysis*; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008297:Male; D008875:Middle Aged; D019798:Neopterin / Q000097:blood; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D007162:Immunoproteins; D018950:Lipopolysaccharide Receptors; D011933:Reagent Kits, Diagnostic; D019798:Neopterin; D002097:C-Reactive Protein