Purification, physicochemical properties, thermal analysis and antinociceptive effect of atranorin extracted from Cladina kalbii.
Lichens and their secondary metabolites have attracted the interest of many researchers. Some species have been shown to contain substances with remarkable biologic activity, as antimicrobial, mainly against Gram positive bacteria; antineoplasic acting on solid and ascetic tumors, or in culture cells; antiviral; hypotensive; and spasmolytic effects. The aim of this study was to isolate and characterize atranorin, one of the major constituents which presents in Cladina kalbii (DES ABB.) AHTI., and analyze its antinociceptive effect. The antinociceptive activity was verified in acetic acid-induced writhing test and formalin test with mice. In this work it was observed that atranorin was effective in significant reducing (p<0.05) abdominal writhing at doses of 200 and 400 mg/kg (p.o.) by 52.6 and 61.3%, respectively, when compared to control group (vehicle). The formalin test showed in 200 and 400 mg/kg (p.o.) that atranorin injection was able to inhibit the inflammatory processes (second phase) dose dependently.
Melo
Marcélia Garcez Dória
MG
Laboratório de Ensaios Farmacêuticos e Toxicidade, Universidade Federal de Sergipe (LeFT/UFS), São Cristóvão-SE, CEP 49000-000, Brazil.
Araújo
Adriano Antunes Souza
AA
Rocha
Carla Paula Leite
CP
Almeida
Emyle Mayra Santana Alves
EM
Siqueira
Rosana de Souza
Rde S
Bonjardim
Leonardo Rigoldi
LR
Quintans
Lucindo José
LJ
D019342:Acetic Acid; D000700:Analgesics / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D001241:Aspirin / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004055:Differential Thermal Analysis; D005557:Formaldehyde; D062385:Hydroxybenzoates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008011:Lichens / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D010147:Pain Measurement / Q000187:drug effects; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
D000700:Analgesics; D062385:Hydroxybenzoates; D005557:Formaldehyde; C026304:atranorin; D019342:Acetic Acid; D001241:Aspirin