Characterization of a Bombyx mori nucleopolyhedrovirus with Bmvp80 disruption.
A BmNPV Bacmid with the Bmvp80 gene disrupted was constructed using the ET-recombination system in Escherichia coli to investigate the role of Bmvp80 during the baculovirus life cycle. Disruption of Bmvp80 resulted in single cell infection phenotype, whereas a rescue BmBacmid restored budded virus titers to wild type levels; however, the homologous gene Ac104 (Acvp80) from AcMNPV could not complement the BmBacmid lacking a functional Bmvp80 gene. Electron microscopy of cells transfected with BmNPV lacking functional Bmvp80 revealed that the number of nucleocapsids was markedly lower. These results suggest that Bmvp80 is essential for normal budded virus production and nucleocapsid maturation, and is functionally divergent between baculovirus species.
Tang
Xu-Dong
XD
Institute of Insect Science, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, Zhejiang, China.
Xu
Yi-Peng
YP
Yu
Lin-Lin
LL
Lang
Guo-Jun
GJ
Tian
Cai-Hong
CH
Zhao
Jin-Fang
JF
Zhang
Chuan-Xi
CX
D000818:Animals; D012831:Bombyx; D002460:Cell Line; D020868:Gene Silencing; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication
D014764:Viral Proteins