title: A limited contribution of Ca2+ current facilitation to paired-pulse facilitation of transmitter release at the rat calyx of Held.
abstract: Recent studies have suggested that transmitter release facilitation at synapses is largely mediated by presynaptic Ca(2+) current facilitation, but the exact contribution of Ca(2+) current facilitation has not been determined quantitatively. Here, we determine the contribution of Ca(2+) current facilitation, and of an increase in the residual free Ca(2+) concentration ([Ca(2+)](i)) in the nerve terminal, to paired-pulse facilitation of transmitter release at the calyx of Held. Under conditions of low release probability imposed by brief presynaptic voltage-clamp steps, transmitter release facilitation at short interstimulus intervals (4 ms) was 227 +/- 31% of control, Ca(2+) current facilitation was 113 +/- 4% of control, and the peak residual [Ca(2+)](i) was 252 +/- 18 nm over baseline. By inferring the 'local' [Ca(2+)](i) transients that drive transmitter release during these voltage-clamp stimuli with the help of a kinetic release model, we estimate that Ca(2+) current facilitation contributes to approximately 40% to paired-pulse facilitation of transmitter release. The remaining component of facilitation strongly depends on the build-up, and on the decay of the residual free [Ca(2+)](i), but cannot be explained by linear summation of the residual free [Ca(2+)](i), and the back-calculated 'local' [Ca(2+)](i) signal, which only accounts for approximately 10% of the total release facilitation. Further voltage-clamp experiments designed to compensate for Ca(2+) current facilitation demonstrated that about half of the observed transmitter release facilitation remains in the absence of Ca(2+) current facilitation. Our results indicate that paired-pulse facilitation of transmitter release at the calyx of Held is driven by at least two distinct mechanisms: Ca(2+) current facilitation, and a mechanism independent of Ca(2+) current facilitation that closely tracks the time course of residual free [Ca(2+)](i).
authors.affiliation: Laboratory of Synaptic Mechanisms, Ecole Polytechnique Fédérale de Lausanne, Brain-Mind Institute, 1015 Lausanne, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Müller
authors.forename: Felix
authors.initials: F
authors.lastname: Felmy
authors.forename: Ralf
authors.initials: R
authors.lastname: Schneggenburger
mesh_terms: D000200:Action Potentials; D000818:Animals; D001306:Auditory Pathways / Q000502:physiology*; D001933:Brain Stem / Q000378:metabolism / Q000502:physiology; D020864:Calcium Channels, N-Type / Q000502:physiology; D020908:Calcium Channels, R-Type / Q000502:physiology; D020013:Calcium Signaling; D004594:Electrophysiology; D066298:In Vitro Techniques; D008959:Models, Neurological; D018377:Neurotransmitter Agents / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000378:metabolism
chemical_list: D020864:Calcium Channels, N-Type; D020908:Calcium Channels, R-Type; D018377:Neurotransmitter Agents