The influence of different hormone concentration and combination on callus induction and regeneration of Rauwolfia serpentina L. Benth.
The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L(-1) BA and 2.0 mg L(-1) NAA. The callus differentiated into adventitious shoots when it was subcultured on MS medium supplemented with 2.0 mg L(-1) BA with 0.2 mg L(-1) NAA. Regenerated shoots were best rooted on half-strength MS medium with 1.0 mg L(-1) each of IBA and IAA.
Salma
U
U
Department of RDDR, Modern Herbal Group, Dhaka-1217, Bangladesh.
Rahman
M S M
MS
Islam
S
S
Haque
N
N
Jubair
T A
TA
Haque
A K M F
AK
Mukti
I J
IJ
D000470:Alkaloids / Q000737:chemistry; D002450:Cell Communication / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D005838:Genotype; D006863:Hydrogen-Ion Concentration; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D011926:Rauwolfia / Q000187:drug effects* / Q000502:physiology; D012038:Regeneration / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D046509:Tissue Culture Techniques
D000470:Alkaloids; D010937:Plant Growth Regulators