title: [The study of dynamic acccumulation of camptothecin in Camptotheca acuminata fruit by TLCS].
abstract: OBJECTIVE
  To establish TLC scanning method for the determination of camptothecin in Camptotheca acuminata fruit, and analysis the dynamic accumulation of camptothecin in Camptotheca acuminata fruit to find out the best collection period.
  
  
  METHODS
  Silica gel H-CMC-Na thin layer plate was adopted in the determination with chloroform-acetone (7 : 3) used as deeloper, Single-wavelength and linear scanning of TLC was used, and the detection wavelength was 360 nm.
  
  
  RESULTS
  There was a good linear relationship for Comptothecin within the range of 0.0542 - 0.3252 microg, the average recovery was 97.13%, RSD was 1.76%.
  
  
  CONCLUSIONS
  The method is accurate, simple and reliable, and can be used for the determination of camptothecin in Camptotheca acuminata fruit and dynamic accumulation research.
authors.affiliation: Nanning Institute for Food and Drug Control, Nanning 530001, China. jnl@nnifdc.com
authors.forename: Ming-Lian
authors.initials: ML
authors.lastname: Jiang
authors.forename: Rui-Song
authors.initials: RS
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.forename: Fang-Li
authors.initials: FL
authors.lastname: Zhou
authors.forename: Qing
authors.initials: Q
authors.lastname: Su
authors.forename: Ai-Yan
authors.initials: AY
authors.lastname: Wei
mesh_terms: D029769:Camptotheca / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D002166:Camptothecin / Q000032:analysis* / Q000378:metabolism; D002855:Chromatography, Thin Layer / Q000379:methods*; D005638:Fruit / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D012621:Seasons; D013997:Time Factors
chemical_list: D002166:Camptothecin