Urinary evaluation of the balance between soluble interferon-gamma receptor (IFN-gammaR1) and interleukin-4 receptor (IL-4Ralpha).
To elucidate the usefulness of the simultaneous analysis of multiple kinds of soluble cytokine receptors in urine specimens, we determined the levels of both the soluble interferon-gamma receptor alpha chain (sIFN-gammaR1, Th1-type cytokine receptor) and the soluble interleukin 4-receptor alpha chain (sIL-4Ralpha, Th2-type cytokine receptor) in the urine of healthy subjects as reference values and preliminarily applied this method to evaluate patients with diarrhea positive (D+) hemolytic uremic syndrome (HUS) as the diagnostic parameters. The urinary sIFN-gammaR levels of children were significantly lower than those of adults (p < 0.01, n = 107). On the other hand, there was no significant difference between the urine sIL-4R levels of adults and children. Statistical correlation between sIFN-gammaR and sIL-4R values was not observed (p = 0.705). On the day of onset of HUS, the urine sIFN-gammaR levels of the patients (n = 6) with HUS were higher than those of the healthy control group (n = 67) (p < 0.01); however, there was no significant difference in the sIL-4R levels between both groups. The urine evaluation of the balance between the soluble cytokine receptors might be informative for the immune states of HUS patients.
Okamoto
Yoshihiro
Y
Laboratory of Immunology and Microbiology, Faculty of Pharmacy, Chiba Institute of Science, 3 Shiomi-cho, Choshi, Chiba 288-0025, Japan. yokamoto@cis.ac.jp
Nakajo
Izumi
I
Seta
Kyoko
K
Gotoh
Yoshimitsu
Y
Nagai
Takuhito
T
Fujita
Naoya
N
Fukui
Takashi
T
Masuzawa
Toshiyuki
T
D000328:Adult; D002648:Child; D005260:Female; D006463:Hemolytic-Uremic Syndrome / Q000276:immunology* / Q000652:urine*; D006801:Humans; D008297:Male; D017471:Receptors, Interferon / Q000032:analysis*; D019948:Receptors, Interleukin-4 / Q000032:analysis*; D016482:Urinalysis; D000096743:Interferon gamma Receptor
D017471:Receptors, Interferon; D019948:Receptors, Interleukin-4; D000096743:Interferon gamma Receptor