Use of ProteinChip technology for identifying biomarkers of parasitic diseases: the example of porcine cysticercosis (Taenia solium).
Taenia solium cysticercosis is a significant public health problem in endemic countries. The current serodiagnostic techniques are not able to differentiate between infections with viable cysts and infections with degenerated cysts. The objectives of this study were to identify specific novel biomarkers of these different disease stages in the serum of experimentally infected pigs using ProteinChip technology (Bio-Rad) and to validate these biomarkers by analyzing serum samples from naturally infected pigs. In the experimental sample set 30 discriminating biomarkers (p<0.05) were found, 13 specific for the viable phenotype, 9 specific for the degenerated phenotype and 8 specific for the infected phenotype (either viable or degenerated cysts). Only 3 of these biomarkers were also significant in the field samples; however, the peak profiles were not consistent among the two sample sets. Five biomarkers discovered in the sera from experimentally infected pigs were identified as clusterin, lecithin-cholesterol acyltransferase, vitronectin, haptoglobin and apolipoprotein A-I.
Deckers
N
N
Department of Animal Health, Institute of Tropical Medicine, Nationalestraat 155, B-2000, Antwerp, Belgium.
Dorny
P
P
Kanobana
K
K
Vercruysse
J
J
Gonzalez
A E
AE
Ward
B
B
Ndao
M
M
D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D002853:Chromatography, Liquid; D051152:Clusterin / Q000097:blood; D003551:Cysticercosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D005260:Female; D006242:Haptoglobins / Q000032:analysis; D008297:Male; D010568:Peru; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood; D040081:Protein Array Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D041201:Taenia solium; D053719:Tandem Mass Spectrometry; D019096:Vitronectin / Q000097:blood; D015024:Zambia
D016632:Apolipoprotein A-I; D015415:Biomarkers; D051152:Clusterin; D006242:Haptoglobins; D019096:Vitronectin; D007862:Phosphatidylcholine-Sterol O-Acyltransferase