title: A simple and rapid CZE method for the analysis of mycophenolic acid and its phenol glucuronide metabolite in human serum.
abstract: A simple and rapid capillary electrophoretic method was developed for simultaneous determination of mycophenolic acid and its metabolite, phenol glucuronide, in human serum. The sample preparation in the proposed method required only the precipitation by acetonitrile. Separation was performed by capillary zone electrophoresis using 75 mM phosphate buffer (pH 7.5) as running buffer, an applied voltage of 10 kV, and UV detection at 217 nm. Each serum sample analysis was completed within 15 min. The optimized method demonstrated good performance concerning specificity, linearity (r>0.9955), accuracy (95.9-113%), precision (<6.4%) and enough sensitivity for therapeutic drug monitoring. This method was successfully applied to measurements of mycophenolic acid and mycophenolic acid glucuronide in renal transplant patient samples and was a useful alternative to high-performance liquid chromatography-based methods.
authors.affiliation: Department of Environmental and Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan. k-ohyama@nagasaki-u.ac.jp
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Kinoshita
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
mesh_terms: D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D020719:Glucuronides / Q000097:blood*; D006801:Humans; D009173:Mycophenolic Acid / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D020719:Glucuronides; D009173:Mycophenolic Acid