A chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix.
One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult.
Toprak
U
U
Molecular Genetics Section, Agriculture and Agri-Food Canada, Saskatoon, SK, Canada.
Baldwin
D
D
Erlandson
M
M
Gillott
C
C
Hou
X
X
Coutu
C
C
Hegedus
D D
DD
D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D030541:Databases, Genetic; D015180:Electrophoresis, Gel, Two-Dimensional; D020224:Expressed Sequence Tags; D015971:Gene Expression Regulation, Enzymologic; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007422:Intestines / Q000201:enzymology*; D007915:Lepidoptera / Q000201:enzymology* / Q000235:genetics; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010802:Phylogeny; D021381:Protein Transport; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment
D019476:Insect Proteins; D011994:Recombinant Proteins; D008049:Lipase; D000581:Amidohydrolases; C019866:chitin deacetylase