title: Genotoxicity of Achillea millefolium essential oil in diploid cells of Aspergillus nidulans.
abstract: The essential oil of Achillea millefolium is commonly used in folk medicine for the treatment of several diseases and has been demonstrated previously to exert an in vitro antimicrobial activity against human pathogens. Current study investigates the genotoxic activity of A. millefolium oil. The oil's major constituents are: chamazulene (42.15%), sabinene (19.72%), terpin-4-ol (5.22%), beta-caryophyllene (4.44%) and eucalyptol (3.10%), comprising 74.63% of the total. The oil's genotoxic evaluation was performed at concentrations of 0.13 microL/mL, 0.19 microL/mL and 0.25 microL/mL with a heterozygous diploid strain of Aspergillus nidulans, named A757//UT448, with green conidia. A statistically significant increasing number of yellow and white mitotic recombinants, per colony, of the diploid strain was reported after oil treatment with 0.19 microL/mL and 0.25 microL/mL concentrations. The genotoxicity of the oil was associated with the induction of mitotic non-disjunction or crossing-over by oil.
authors.affiliation: Universidade Estadual de Maringá, Departamento de Biologia Celular e Genética, Avenida Colombo 5790, Maringá PR Brazil 87020-900.
authors.forename: Juliane Rocha
authors.initials: JR
authors.lastname: de Sant'anna
authors.forename: Claudinéia Conationi da Silva
authors.initials: CC
authors.lastname: Franco
authors.forename: Claudia Tiemi
authors.initials: CT
authors.lastname: Miyamoto
authors.forename: Miriam Machado
authors.initials: MM
authors.lastname: Cunico
authors.forename: Obdulio Gomes
authors.initials: OG
authors.lastname: Miguel
authors.forename: Lílian Cristina
authors.initials: LC
authors.lastname: Côcco
authors.forename: Carlos Itsuo
authors.initials: CI
authors.lastname: Yamamoto
authors.forename: Cirino Corrêa
authors.initials: CC
authors.lastname: Junior
authors.forename: Marialba Avezum Alves
authors.initials: MA
authors.lastname: de Castro-Prado
mesh_terms: D031162:Achillea / Q000737:chemistry*; D001233:Aspergillus nidulans / Q000187:drug effects* / Q000235:genetics; D003434:Crossing Over, Genetic / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D008519:Medicine, Traditional; D009152:Mutagenicity Tests; D009630:Nondisjunction, Genetic / Q000187:drug effects; D009822:Oils, Volatile / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*
chemical_list: D009822:Oils, Volatile; D010938:Plant Oils