129/SvJ mice have mutated CD23 and hyper IgE.
CD23, the low affinity IgE receptor, is hypothesized to function as a negative regulator of IgE production. Upon discovering reduced CD23 surface levels in 129/SvJ inbred mice, we sought to further investigate 129/SvJ CD23 and to examine its influence on IgE levels. Five amino acid substitutions were found in 129/SvJ CD23. Identical mutations were also observed in CD23 from New Zealand Black and 129P1/ReJ mice. 129/SvJ B cells proliferated more rapidly than those from BALB/c after stimulation with IL-4 and CD40 ligand trimer. However, in vitro IgE levels in supernatants from stimulated 129/SvJ B cells were significantly reduced. Contrary to the in vitro findings, the 129/SvJ CD23 mutations correlated with a hyper IgE phenotype in vivo and 129/SvJ were able to clear Nippostrongylus brasiliensis infection more rapidly than either BALB/c or C57BL/6. Overall, this study further suggests that CD23 is an important regulatory factor for IgE production.
Ford
Jill W
JW
Virginia Commonwealth University, Department of Microbiology and Immunology, P.O. Box 980678, MCV Station, Richmond, VA 23298, USA.
Sturgill
Jamie L
JL
Conrad
Daniel H
DH
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001665:Binding Sites; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D017124:Conserved Sequence; D005786:Gene Expression Regulation; D007073:Immunoglobulin E / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017455:Receptors, IgE / Q000097:blood / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D016415:Sequence Alignment
D012333:RNA, Messenger; D017455:Receptors, IgE; D007073:Immunoglobulin E