title: Multiplexed multicolor Raman imaging of live cells with isotopically modified single walled carbon nanotubes.
abstract: We show that single walled carbon nanotubes (SWNTs) with different isotope compositions exhibit distinct Raman G-band peaks and can be used for multiplexed multicolor Raman imaging of biological systems. Cancer cells with specific receptors are selectively labeled with three differently "colored" SWNTs conjugated with various targeting ligands including Herceptin (anti-Her2), Erbitux (anti-Her1), and RGD peptide, allowing for multicolor Raman imaging of cells in a multiplexed manner. SWNT Raman signals are highly robust against photobleaching, allowing long-term imaging and tracking. With narrow peak features, SWNT Raman signals are easily differentiated from the autofluorescence background. The SWNT Raman excitation and scattering photons are in the near-infrared region, which is the most transparent optical window for biological systems in vitro and in vivo. Thus, SWNTs are novel Raman tags promising for multiplexed biological detection and imaging.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California 94305, USA.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Li
authors.forename: Scott M
authors.initials: SM
authors.lastname: Tabakman
authors.forename: Kaili
authors.initials: K
authors.lastname: Jiang
authors.forename: Shoushan
authors.initials: S
authors.lastname: Fan
authors.forename: Hongjie
authors.initials: H
authors.lastname: Dai
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D003116:Color; D015394:Molecular Structure; D037742:Nanotubes, Carbon / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D037742:Nanotubes, Carbon