title: Technical viability of the production, partial purification and characterisation of inulinase using pretreated agroindustrial residues.
abstract: The present work aimed to study the viability of the use of sugarcane molasses and corn steep liquor (CSL) in a sequential inulinase production performing an up-stream pretreatment of these agroindustrial residues. A sequential strategy was used applying three central composite rotatable designs (CCRDs) to optimise medium composition, followed by a down-stream step. The medium containing 150 g L(-1) molasses, 50 g L(-1) CSL and 6 g L(-1) yeast extract, yielded a maximum inulinase production of 1,294 +/- 7 U mL(-1), after 72 h of fermentation. A down-stream evaluation was carried out using an expanded bed of Streamline DAE resin (Pharmacia), with and without the up-stream treatment. The results showed that the enzyme could not be recovered from the non-pretreated medium, whereas a yield of 91% was obtained in the adsorption stage from the medium prepared with the up-stream treatment, showing the viability of producing the enzyme inulinase from agroindustrial residues using the integrated process.
authors.affiliation: Department of Food Engineering, URI, Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, RS, 99700-000, Brazil. helen@uricer.edu.br
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.forename: Marcio A
authors.initials: MA
authors.lastname: Mazutti
authors.forename: Francisco
authors.initials: F
authors.lastname: Maugeri Filho
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Rodrigues
mesh_terms: D001698:Biomedical Engineering; D019149:Bioreactors; D001709:Biotechnology; D003470:Culture Media; D004795:Enzyme Stability; D005285:Fermentation; D005524:Food Technology; D005632:Fructose / Q000096:biosynthesis; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007444:Inulin / Q000378:metabolism; D007700:Kinetics; D007716:Kluyveromyces / Q000201:enzymology / Q000254:growth & development; D008965:Molasses; D009844:Oligosaccharides / Q000096:biosynthesis; D013816:Thermodynamics; D003313:Zea mays
chemical_list: D003470:Culture Media; D009844:Oligosaccharides; C116580:fructooligosaccharide; D005632:Fructose; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase