Consequences of in vitro culture conditions on embryo development and quality.
Despite major efforts directed at improving the yield of blastocysts from immature oocytes in vitro, the quality of such blastocysts continually lags behind that of blastocysts produced in vivo. These differences are manifested at the level of morphology, metabolism, gene expression and cryotolerance, and may have a knock-on effect further along the developmental axis. Evidence suggesting that in vitro culture conditions, while capable of producing blastocysts in relatively high numbers, are far from optimal with deficiencies being manifested in terms of abnormally large offspring. It is clear nowadays that modification of the post-fertilization culture environment in vitro can improve blastocyst quality to some extent.
Rizos
D
D
Dpto Reproducción Animal, INIA, Madrid, Spain. drizos@inia.es
Clemente
M
M
Bermejo-Alvarez
P
P
de La Fuente
J
J
Lonergan
P
P
Gutiérrez-Adán
A
A
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D015925:Cryopreservation / Q000662:veterinary; D046149:Embryo Culture Techniques / Q000379:methods / Q000662:veterinary*; D004624:Embryo Transfer / Q000662:veterinary; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D015870:Gene Expression; D012743:Sex Preselection / Q000662:veterinary