Production of phenylpropanoid amides in recombinant Escherichia coli.
Plant-specific phenylpropanoid amides were produced in a recombinant Escherichia coli that expressed 4-coumarate:coenzyme A ligase from Arabidopsis and serotonin N-hydroxycinnamoyltransferase from pepper plants. Upon exogenous treatment with several precursors, high concentrations of the following phenylpropanoid amides were produced abundantly in the culture medium in a few hours: 4-coumaroylserotonin (215 mg/l), 4-coumaroyloctopamine (208 mg/l), and 4-coumaroyltyramine (187 mg/l).
Kang
Kiyoon
K
Department of Biotechnology, Agricultural Plant Stress Research Center, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
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Kyoungwhan
K
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D002212:Capsicum / Q000201:enzymology* / Q000235:genetics; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011422:Propionates
D003373:Coumaric Acids; D010940:Plant Proteins; D011422:Propionates; D003066:Coenzyme A Ligases; C495469:p-coumaric acid