Double immunization strategy with a BoHV-4-vectorialized secreted chimeric peptide BVDV-E2/BoHV-1-gD.
A bovine herpesvirus 4 was isolated from the milk cell fraction of a healthy cow and his full genome cloned as a bacterial artificial chromosome. So cloned viral genome was used as a vector platform to deliver in vitro and in vivo an optimized secreted chimeric peptide obtained by the fusion of the bovine viral diarrhoea virus glycoprotein E2 ectodomain with the bovine herpesvirus 1 glycoprotein D ectodomain. Recombinant virus infected cells robustly expressed and secreted the chimeric peptide into the culture medium and inoculated animals with the recombinant virus successfully responded toward antigens, gE2 and gD. Thus, this work has implications for the development of safe and effective polyvalent vaccines.
Donofrio
G
G
Università di Parma, Facoltà di Medicina Veterinaria, Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali, via del Taglio 10, Parma, Italy. gaetano.donofrio@unipr.it
Sartori
C
C
Franceschi
V
V
Capocefalo
A
A
Cavirani
S
S
Taddei
S
S
Flammini
C F
CF
D000818:Animals; D015153:Blotting, Western; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology / Q000517:prevention & control; D002417:Cattle; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D030241:Diarrhea Virus 1, Bovine Viral / Q000235:genetics* / Q000276:immunology*; D005822:Genetic Vectors; D007242:Herpesvirus 1, Bovine / Q000276:immunology; D026021:Herpesvirus 4, Bovine / Q000235:genetics* / Q000276:immunology*; D007114:Immunization / Q000379:methods*; D017403:In Situ Hybridization; D008156:Luciferases / Q000235:genetics; D016254:Mutagenesis, Insertional; D050506:Mutant Chimeric Proteins / Q000235:genetics* / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology*
D004279:DNA, Viral; D050506:Mutant Chimeric Proteins; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins; C076551:gp53, bovine viral diarrhea virus; D008156:Luciferases