title: Effects of high temperature on multiple parameters of broilers in vitro and in vivo.
abstract: The effects of high temperature on multiple parameters of broilers were evaluated both in vitro and in vivo. In the in vitro study, the bilateral musculus fibularis longus muscles of 8 broilers were isolated and incubated at either 41.5 or 44.5 degrees C. The greater incubation temperature increased mitochondrial H2O2 production by 28.0% (P < 0.0001), malondialdehyde concentration by 16.8% (P = 0.0368), and lactate concentration by 33.0% (P < 0.0001) and decreased mitochondrial Ca2+-ATPase activity by 19.6% (P = 0.0001). In the in vivo study, 180 four-week-old broilers were kept in 3 controlled-environment chambers for 3 wk. High temperature increased mitochondrial H2O2 production (P < 0.05) in liver, malondialdehyde concentration in liver and breast muscle, and lactate concentration in breast muscle (P < 0.05). In addition, it inhibited mitochondrial Ca2+-ATPase activity in muscle and liver (P < 0.05). High temperature also significantly decreased initial pH and increased L*, drip loss, and shear force of broiler breast muscle.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Science, State Key Laboratory of Animal Nutrition, Beijing, 100094, China.
authors.forename: J
authors.initials: J
authors.lastname: Feng
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Zheng
authors.forename: P
authors.initials: P
authors.lastname: Xie
authors.forename: A
authors.initials: A
authors.lastname: Ma
mesh_terms: D000064:Acclimatization; D000822:Animal Husbandry; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002645:Chickens / Q000502:physiology*; D018882:Heat Stress Disorders / Q000503:physiopathology / Q000662:veterinary*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology*; D008460:Meat / Q000592:standards; D008931:Mitochondria, Muscle / Q000378:metabolism; D018482:Muscle, Skeletal / Q000503:physiopathology; D011201:Poultry Diseases / Q000503:physiopathology
chemical_list: D006861:Hydrogen Peroxide; D000252:Calcium-Transporting ATPases