Phosphorescent thymidine triphosphate sensor based on a donor-acceptor ensemble system using intermolecular energy transfer.
An ensemble sensor system that exhibited selective luminescence enhancement upon binding to thymidine 5'-triphosphate (TTP) in HEPES buffer over other nucleotides was developed. The ensemble system consisted of an energy acceptor (FIrpic-bis(Zn2+-dipicolylamine conjugate, FIrpic=bis[(4,6-difluorophenyl)-pyridinato-N,C2+]picolinate) derivative) and an energy donor (mCP-Zn2+-cyclen, mCP=N,N'-dicarbazolyl-3,5-benzene). Among the nucleotides, the selective recognition and luminescence enhancement for TTP was achieved by the strong binding of the thymine unit to Zn2+-cyclen (cyclen=1,4,7,10-tetraazacyclododecane) and intermolecular energy transfer between the mCP and FIrpic moieties.
Kwon
Tae-Hyuk
TH
Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747 (Korea).
Kim
Hee Jin
HJ
Hong
Jong-In
JI
D000478:Alkylation; D004735:Energy Transfer; D007783:Lactones / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D010758:Phosphorus / Q000737:chemistry*; D010777:Photochemistry; D013237:Stereoisomerism; D013942:Thymine Nucleotides / Q000737:chemistry*
D007783:Lactones; D013942:Thymine Nucleotides; D010758:Phosphorus; C024157:thymidine 5'-triphosphate