title: Division protein interaction web: identification of a phylogenetically conserved common interactome between Streptococcus pneumoniae and Escherichia coli.
abstract: The ability of each of the 11 Streptococcus pneumoniae division proteins to interact with itself and with each of the remaining proteins was studied in 66 combinations of protein pairs, using a bacterial two-hybrid system. Interactions (homo- or hetero-dimerizations) were detected between 37 protein pairs, whereas 29 protein pairs did not interact. In some cases, positive interactions of the S. pneumoniae proteins were confirmed by co-immunoprecipitation experiments in Escherichia coli. Comparison between the S. pneumoniae division protein interaction web and that of E. coli, the only micro-organisms for which the whole division interactome has been described systematically, was also performed. At least nine division proteins, ZapA, FtsZ, FtsA, FtsK, FtsQ/DivIB, FtsB/DivIC, FtsL, FtsI and FtsW, are believed to have a conserved function between these bacteria and thus we may say that a significant part of the interactions are conserved. Out of 45 protein pairs tested in both bacteria, 30 showed the same behaviour: 23 interacted while seven did not. In agreement with these results, cross-interactions between S. pneumoniae proteins and the corresponding E. coli orthologues were observed. Taken together, these results suggest a phylogenetically conserved minimal common interactome of the division proteins.
authors.affiliation: Dipartimento di Biologia, Università Tor Vergata, Roma, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Maggi
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Sez. Microbiologia Medica, Cagliari, Italy.
authors.forename: Orietta
authors.initials: O
authors.lastname: Massidda
authors.affiliation: Dipartimento di Medicina Interna, Facoltà di Medicina, Università La Sapienza, Roma, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Luzi
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Sez. Microbiologia Medica, Cagliari, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fadda
authors.affiliation: Dipartimento di Biologia, Università Tor Vergata, Roma, Italy.
authors.forename: Luciano
authors.initials: L
authors.lastname: Paolozzi
authors.affiliation: Istituto di Biologia e Patologia Molecolare del CNR, Roma, Italy.|Dipartimento di Biologia, Università Tor Vergata, Roma, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Ghelardini
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D002455:Cell Division; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D047468:Immunoprecipitation; D010802:Phylogeny; D010957:Plasmids; D025941:Protein Interaction Mapping; D020543:Proteome / Q000235:genetics; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000235:genetics*; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D020543:Proteome