Fluctuation of annexin-A1 positive mast cells in chronic granulomatous inflammation.
OBJECTIVE
We have applied here a model of chronic granulomatous inflammation to study the profile of mast cell activation and their expression of annexin-A1 in the nodular lesion.


METHODS
Granulomatous inflammation was induced by injection of croton oil and Freund's complete adjuvant (CO/FCA) into the dorsal air-pouches of mice. Skin tissue samples were collected from control group (24 h time-point; i. e. before disease development) and 7, 14, 21, 28 and 42 days post-CO/FCA treatment.


RESULTS
Histopathological analyses revealed an on-going inflammation characterized by an increased number of activated mast cells at sites of the chronic inflammatory reaction in all experimental groups. Immunohistochemical analysis showed skin mast cells highly immunoreactive for annexin-A1, both at an initial (day 7) and a delayed (day 28) phase of the inflammatory reaction.


CONCLUSIONS
The observed time-dependent modulation of mast cell activation, during the granulomatous injury, indicates that multiple pathways centred on annexin-A1 might become activated at different stages of this chronic inflammatory response, including the delayed and pro-resolving phase.
Oliani
S M
SM
Department of Biology, Instituto de Biociências, Letras e Ciências Exatas (IBILCE), São Paulo State University - UNESP, Rua Cristóvão Colombo, 2265, São José do Rio Preto, SP, 15054-000, Brazil. smoliani@ibilce.unesp.br
Ciocca
G A Panin
GA
Pimentel
T A
TA
Damazo
A S
AS
Gibbs
L
L
Perretti
M
M
D000818:Animals; D017305:Annexin A1 / Q000378:metabolism*; D002908:Chronic Disease; D003436:Croton Oil / Q000494:pharmacology; D003879:Dermatologic Agents / Q000494:pharmacology; D005260:Female; D006099:Granuloma / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology*; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D012867:Skin / Q000033:anatomy & histology / Q000378:metabolism / Q000473:pathology
D017305:Annexin A1; D003879:Dermatologic Agents; D003436:Croton Oil