Role of class I human leukocyte antigen molecules in early steps of echovirus infection of rhabdomyosarcoma cells.
Several echoviruses use decay accelerating factor (DAF) as a cell surface receptor. However, most of them require additional cell surface coreceptors. We investigated the respective roles of DAF and class I human leukocyte antigen (HLA) molecules in the early steps of the echovirus 11 (EV11) lifecycle in rhabdomyosarcoma (RD) cells. EV11 infection was inhibited at an early stage by anti-beta2-microglobulin (beta2m) and anti-HLA monoclonal antibodies and by a soluble monochain HLA class I molecule. Expression of class I HLA molecules restored the early steps of the EV11 lifecycle, but its expression was not sufficient for EV11 replication and particle production. Expression of HLA class I molecules was associated with leukocyte cell line permissiveness to EV11 infection. In conclusion, HLA class I molecules are involved in the early steps of EV11 infection of RD cells and appear to participate in a complex interplay of surface molecules acting as coreceptors, including DAF.
Chevaliez
Stéphane
S
Unité de Biologie des Virus Entériques, Institut Pasteur, Paris, France. stephane.chevaliez@hmn.aphp.fr
Balanant
Jean
J
Maillard
Patrick
P
Lone
Yu-Chun
YC
Lemonnier
Francois A
FA
Delpeyroux
Francis
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018958:CD55 Antigens / Q000378:metabolism; D016466:CHO Cells; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D004457:Echovirus Infections / Q000378:metabolism*; D029822:Enterovirus B, Human / Q000378:metabolism* / Q000502:physiology; D005786:Gene Expression Regulation; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism / Q000821:virology; D011485:Protein Binding / Q000187:drug effects; D012208:Rhabdomyosarcoma / Q000378:metabolism*; D014779:Virus Replication; D001613:beta 2-Microglobulin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D018958:CD55 Antigens; D015395:Histocompatibility Antigens Class I; D001613:beta 2-Microglobulin