Comparative study of interleukin-1beta expression by peripheral blood mononuclear cells and purified monocytes experimentally infected with Burkholderia pseudomallei and Burkholderia thailandensis.
Burkholderia pseudomallei is a causative agent of melioidosis. The present study investigated IL-1beta mRNA and protein expression by peripheral blood mononuclear cells and purified monocytes (n = 10) in response to infection with B. pseudomallei and B. thailandensis. Similarly increased IL-1beta mRNA and protein expression was found in both PBMC and purified monocytes stimulated with B. pseudomallei and B. thailandensis. Thus, this study suggests that IL-1beta response does not differ between infections with B. pseudomallei and its non-virulent counterpart and other mechanisms may be involved in their distinct virulence in causing the disease.
Pongcharoen
Sutatip
S
Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand. sutatipp@nu.ac.th
Niumsup
Pannika Ritvirool
PR
Butkhamchot
Puntharee
P
D019117:Burkholderia / Q000276:immunology*; D016957:Burkholderia pseudomallei / Q000276:immunology* / Q000472:pathogenicity; D002478:Cells, Cultured; D006801:Humans; D053583:Interleukin-1beta / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D012333:RNA, Messenger / Q000378:metabolism
D053583:Interleukin-1beta; D012333:RNA, Messenger