[Molecular and biologic characteristics of attenuated rubella viruses].
OBJECTIVE
To study stability/variability of rubella virus vaccine strain "Orlov-B" during its adaptation to other tissue substrate.


METHODS
Vaccine strains of rubella virus Wistar 27/3 and "Orlov-B" as well as wild type strains "Orlov-D" and "Lebedev" were used. Rhesus monkeys were used as laboratory animals. Standard virological, molecular and statistical methods were applied.


RESULTS
Obtained as a result of adaptation to other tissue substrate - diploid human cell line M-22 - strain "Orlov-D" demonstrated stability on RCT40 sign in in vitro experiments. Comparative genotyping of "Orlov-B" and "Orlov-D" strains on gene E1 showed identity of nucleotide sequences of both variants. Genetic stability of virus on the gene coding the most immunogenic protein E1 was confirmed in vivo: the stable high immunogenic and protective activity of both "Orlov-B" and "Orlov- D" strains was demonstrated in experiments on rhesus macaques.


CONCLUSIONS
New data on stability of attenuated rubella virus vaccine strains have practical significance for the development of new vaccines.
Lavrent'eva
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D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D005814:Genes, Viral; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007273:Injections, Intramuscular; D008253:Macaca mulatta; D008297:Male; D011817:Rabbits; D012409:Rubella / Q000276:immunology / Q000821:virology*; D012411:Rubella Vaccine / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D012412:Rubella virus / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D012692:Serial Passage; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics; D014774:Virulence; D014776:Virus Cultivation
D000914:Antibodies, Viral; D012411:Rubella Vaccine; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; C061826:E1 envelope protein, Rubella virus