Cellular uptake of gold nanoparticles directly cross-linked with carrier peptides by osteosarcoma cells.
Nanoparticles have been extensively used for a variety of biomedical applications and there is a growing need for highly specific and efficient delivery of the nanoparticles into target cells and subcellular location. We attempted to accomplish this goal by modifying gold particles with peptide motif's that are known to deliver a 'cargo' into chosen cellular location specifically, we intended to deliver nanogold particles into cells through chemical cross-linking with different peptides known to carry protein into cells. Our results suggest that specific sequence of such 'carrier peptides' can efficiently deliver gold nanoparticles into cells when chemically cross-linked with the metal particles.
Mandal
Deendayal
D
Department of Orthopedics, Mayo Clinic and Foundation, Rochester, MN 55905, USA. ddmandal@yahoo.com
Maran
Avudaippan
A
Yaszemski
Michael J
MJ
Bolander
Mark E
ME
Sarkar
Gobinda
G
D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000737:chemistry / Q000493:pharmacokinetics; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000737:chemistry* / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D003432:Cross-Linking Reagents; D016503:Drug Delivery Systems; D006046:Gold / Q000737:chemistry* / Q000493:pharmacokinetics*; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals / Q000737:chemistry / Q000493:pharmacokinetics; D012516:Osteosarcoma / Q000378:metabolism* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry* / Q000493:pharmacokinetics*; D013347:Subcellular Fractions / Q000378:metabolism / Q000648:ultrastructure
D001672:Biocompatible Materials; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D019913:Nuclear Localization Signals; D010455:Peptides; D006046:Gold