title: Computational search for over-represented 8-mers within the 5'-regulatory regions of 634 mouse testis-specific genes.
abstract: Accumulation of microarray data has enabled the computational analysis of gene expressions in various tissues. Although the genes showing testis-specific expression are most abundant among the genes exhibiting tissue-specific expression, no systematic study has been conducted for over-represented motifs within their regulatory regions. We have identified 117 over-represented 8-mers that appeared 2648 times within the regulatory regions of 634 testis-specific genes. Of these, 64 over-represented 8-mers were significantly more frequent in the regulatory regions of testis-specific genes than in those of non-testis-specific genes. In this group of 8-mers, 4 8-mers differed from the canonical cAMP response element (CRE) 8-mer by 1 letter, but the canonical CRE was not included in this group. We consider that CRE-like 8-mers participate in the regulatory expression of testis-specific genes to a greater extent than the canonical CRE 8-mer.
authors.affiliation: Department of Genome Informatics, Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Yamashita
authors.forename: Naohisa
authors.initials: N
authors.lastname: Goto
authors.forename: Seiji
authors.initials: S
authors.lastname: Nishiguchi
authors.forename: Kazunori
authors.initials: K
authors.lastname: Shimada
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yamanishi
authors.forename: Teruo
authors.initials: T
authors.lastname: Yasunaga
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D000242:Cyclic AMP / Q000378:metabolism*; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D008954:Models, Biological; D008957:Models, Genetic; D015233:Models, Statistical; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D020218:Response Elements; D013737:Testis / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000242:Cyclic AMP