title: Schistosoma mansoni: Microarray analysis of gene expression induced by host sex.
abstract: Schistosoma mansoni is a digenetic trematode and a human parasite responsible for high social and economic impact. Although some authors have studied the effect of host hormones on parasites, not much is known about the effects of host sex on gene expression in Schistosomes. In order to study gene transcripts associated with the host sex, we compared the gene expression profiles of both male and female unisexual adult S. mansoni parasites raised on either male or female hosts, using DNA microarrays. Our results show that host sex caused differential expression of at least 11 genes in female parasites and of 134 in male parasites. Of the differentially expressed genes in female worms, 10 were preferentially expressed in female worms from male mice, while of the 134 differentially expressed genes in male parasites, 79 (59%) were preferentially expressed in worms from female mice. Further investigation of the role of each of those genes will help understand better their importance in the pathogenesis of Schistosomiasis.
authors.affiliation: Laboratório de Genética Bioquímica, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Waisberg
authors.forename: F P
authors.initials: FP
authors.lastname: Lobo
authors.forename: G C
authors.initials: GC
authors.lastname: Cerqueira
authors.forename: L K J
authors.initials: LK
authors.lastname: Passos
authors.forename: O S
authors.initials: OS
authors.lastname: Carvalho
authors.forename: N M
authors.initials: NM
authors.lastname: El-Sayed
authors.forename: G R
authors.initials: GR
authors.lastname: Franco
mesh_terms: D000818:Animals; D001700:Biomphalaria; D005260:Female; D015870:Gene Expression; D006790:Host-Parasite Interactions; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D018510:RNA, Helminth / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000235:genetics*; D012555:Schistosomiasis mansoni / Q000469:parasitology*; D012737:Sex Factors
chemical_list: D018510:RNA, Helminth