Study on the interaction of morphine chloride with deoxyribonucleic acid by fluorescence method.
The mode and mechanism of the interaction of morphine chloride, an important alkaloid compound to calf thymus deoxyribonucleic acid (ct DNA) was investigated from absorption and fluorescence titration techniques. Hypochromic effect was founded in the absorption spectra of morphine when concentration of DNA increased. The decreased fluorescence study revealed non-cooperative binding of the morphine to DNA with an affinity of 3.94x10(3)M(-1), and the stoichiometry of binding was characterized to be about one morphine molecule per nucleotide. Stern-Volmer plots at different temperatures proved that the quenching mechanism was static. Ferrocyanide quenching study showed that the magnitude of K(SV) of the bound morphine was lower than that of the free one. In addition, it was found that ionic strength could affect the binding of morphine and DNA. Fluorescence polarization and denatured DNA studies also applied strong evidences that morphine molecule was partially intercalated between every alternate base pairs of ct DNA. As observed from above experiments, intercalation was well supported as the binding mode of morphine and ct DNA.
Li
J F
JF
Research Center of Environmental Science and Engineering, Department of Chemistry, Shanxi University, Taiyuan 030006, PR China.
Dong
C
C
D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005454:Fluorescence Polarization; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D009020:Morphine / Q000737:chemistry / Q000493:pharmacokinetics*; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009994:Osmolar Concentration; D013050:Spectrometry, Fluorescence; D017186:Titrimetry
D007364:Intercalating Agents; D009020:Morphine; D004247:DNA