Matrix metalloproteinases.
Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods--cell-mediated dissolution of type-1 collagen fibrils, direct and reverse zymography, enzyme capture based on alpha2-macroglobulin and TIMP-1 and -2, and demonstration of cryptic thiol groups in metalloproteinase precursors--that are used to characterize the functions of matrix metalloproteinases and their inhibitors.
Birkedal-Hansen
Henning
H
National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, USA.
Yamada
Susan
S
Windsor
Jack
J
Pollard
Anne Havernose
AH
Lyons
Guy
G
Stetler-Stevenson
William
W
Birkedal-Hansen
Bente
B
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D003094:Collagen / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling; D013710:Tendons / Q000378:metabolism; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism
D061965:Matrix Metalloproteinase Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D000511:alpha-Macroglobulins; D003094:Collagen; D020782:Matrix Metalloproteinases