Anti-free radical activities of kaempferol isolated from Acacia nilotica (L.) Willd. Ex. Del.
In the present study the polyphenolic compound has been isolated from methanol extract of Acacia nilotica Willd. Ex. Del. which has been identified as kaempferol (AN-5) by NMR and mass spectroscopy. The antioxidant potential of the AN-5 was demonstrated in several in vitro assays: measuring the proton radical scavenging activity (DPPH scavenging assay), hydroxyl radical scavenging activity (deoxyribose degradation assay), metal chelating activity, reducing power and inhibition of lipid peroxidation. It was found that the effect of the compound AN-5 was strongly dose dependent up to the concentrations 1-50 microg/ml in DPPH assay and 1-100 microg/ml in deoxyribose degradation assay but did not show further change above the highest concentrations.
Singh
Rajbir
R
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar-143 005, Punjab, India.
Singh
Bikram
B
Singh
Sukhpreet
S
Kumar
Neeraj
N
Kumar
Subodh
S
Arora
Saroj
S
D000045:Acacia / Q000737:chemistry*; D001713:Biphenyl Compounds; D002614:Chelating Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D003849:Deoxyguanosine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D044949:Kaempferols / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010851:Picrates; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology
D001713:Biphenyl Compounds; D002614:Chelating Agents; D016166:Free Radical Scavengers; D044949:Kaempferols; D010851:Picrates; D010936:Plant Extracts; C006552:kaempferol; C004931:1,1-diphenyl-2-picrylhydrazyl; D003849:Deoxyguanosine