title: A combined AFLP and microsatellite linkage map and pilot comparative genomic analysis of European sea bass Dicentrarchus labrax L.
abstract: European sea bass (Dicentrarchus labrax L., Moronidae, Teleostei) sustains a regional fishery and is commonly farmed in the Mediterranean basin, but has not undergone much long-term genetic improvement. An updated genetic linkage map of the European sea bass was constructed using 190 microsatellites, 176 amplified fragment length polymorphisms and two single nucleotide polymorphisms. From the 45 new microsatellite markers (including 31 type I markers) reported in this study, 28 were mapped. A total of 368 markers were assembled into 35 linkage groups. Among these markers, 28 represented type I (coding) markers, including those located within the peptide Y, SOX10, PXN1, ERA and TCRB genes (linkage groups 1, 7, 16, 17 and 27 respectively). The sex-averaged map spanned 1373.1 centimorgans (cM) of the genome. The female map measured 1380.0 cM, whereas the male map measured 1046.9 cM, leading to a female-to-male (F:M) recombination rate ratio of 1.32:1. The intermarker spacing of the second-generation linkage map of the European sea bass was 3.67 cM, which is smaller than that of the first-generation linkage map (5.03 cM). Comparative mapping of microsatellite flanking regions was performed with five model teleosts and this revealed a high percentage (33.6%) of evolutionarily conserved regions with the three-spined stickleback.
authors.affiliation: Laboratory of Animal Diversity and Systematics, Katholieke Universiteit Leuven, Ch. Deberiotstraat 32, B-3000 Leuven, Belgium. dimitry.chistiakov@eudoramail.com
authors.forename: D A
authors.initials: DA
authors.lastname: Chistiakov
authors.forename: C S
authors.initials: CS
authors.lastname: Tsigenopoulos
authors.forename: J
authors.initials: J
authors.lastname: Lagnel
authors.forename: Y-M
authors.initials: YM
authors.lastname: Guo
authors.forename: B
authors.initials: B
authors.lastname: Hellemans
authors.forename: C S
authors.initials: CS
authors.lastname: Haley
authors.forename: F A M
authors.initials: FA
authors.lastname: Volckaert
authors.forename: G
authors.initials: G
authors.lastname: Kotoulas
mesh_terms: D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics*; D017931:DNA Primers; D008040:Genetic Linkage; D023281:Genomics; D018895:Microsatellite Repeats / Q000235:genetics*; D010865:Pilot Projects; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers