title: Demonstration of improvements to the bioluminescence resonance energy transfer (BRET) technology for the monitoring of G protein-coupled receptors in live cells.
abstract: The bioluminescence resonance energy transfer (BRET) technique has become extremely popular for studying protein-protein interactions in living cells and real time. Of particular interest is the ability to monitor interactions between G protein-coupled receptors, such as the thyrotropin-releasing hormone receptor (TRHR), and proteins critical for regulating their function, such as beta-arrestin. Using TRHR/beta-arrestin interactions, we have demonstrated improvements to all 3 generations of BRET (BRET(1), BRET(2), and eBRET) by using the novel forms of luciferase, Rluc2 and Rluc8, developed by the Gambhir laboratory. Furthermore, for the 1st time it was possible to use the BRET2 system to detect ligand-induced G protein-coupled receptor/beta-arrestin interactions over prolonged periods (on the scale of hours rather than seconds) with a very stable signal. As demonstrated by our Z'-factor data, these luciferases increase the sensitivity of BRET to such an extent that they substantially increase the potential applicability of this technology for effective drug discovery high-throughput screening.
authors.affiliation: Laboratory for Molecular Endocrinology-GPCRs, Western Australian Institute for Medical Research (WAIMR), Centre for Medical Research, University of Western Australia, Perth, Western Australia, Australia.
authors.forename: Martina
authors.initials: M
authors.lastname: Kocan
authors.forename: Heng B
authors.initials: HB
authors.lastname: See
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Seeber
authors.forename: Karin A
authors.initials: KA
authors.lastname: Eidne
authors.forename: Kevin D G
authors.initials: KD
authors.lastname: Pfleger
mesh_terms: D002460:Cell Line; D015195:Drug Design; D055808:Drug Discovery; D004735:Energy Transfer; D006801:Humans; D007295:Inositol Phosphates / Q000737:chemistry; D007700:Kinetics; D008156:Luciferases / Q000737:chemistry; D008164:Luminescent Proteins / Q000737:chemistry; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry*; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013997:Time Factors
chemical_list: D007295:Inositol Phosphates; D008164:Luminescent Proteins; D043562:Receptors, G-Protein-Coupled; D018025:Receptors, Thyrotropin-Releasing Hormone; D011993:Recombinant Fusion Proteins; D008156:Luciferases