[Studies on active ingredients in Corydalis, broken cell wall corydalis and its processed products].
OBJECTIVE
To compare the contents of tetrahydropalmatine and dehydrocorydaline in corydalis, broken cell wall corydalis and its different processed products.


METHODS
The broken cell wall technique was used to corydalis, and then both the corydalis and broken cell wall corydalis were processed. The method of chromatography which was used to determine the contents of tetrahydropalmatine and dehydrocorydaline in corydalis broken cell wall corydalis and its different processed products was performed by RP-HPLC with Kromasil ODS-C18 (4.6 mm x 250 mm, 5 microm) column was used at 35 degrees C, acetonitrile-acetate buffer solution (pH 6.0) (30:70) as mobile phase of 1 mL x min(-1) flow rate, detection wavelength was set at 280 nm.


RESULTS
The contents of the two active components in broken cell wall corydalis were higher than that in corydalis, while that in broken cell wall and vinegar-fried corydalis was highest in the products of breaked cell wall corydalis.


CONCLUSIONS
Breaking the cell wall of corydalis can help to dissolute alkaloids.
Cao
Liu
L
Tianjin University of Traditional Chinese Medicine University, Tianjin 300193, China.
Diu
Zhi-Ying
ZY
Wang
Ping
P
Sun
Wei
W
Tian
Yong-Liang
YL
D000470:Alkaloids / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D002473:Cell Wall; D002851:Chromatography, High Pressure Liquid; D031311:Corydalis / Q000737:chemistry* / Q000166:cytology*; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
D000470:Alkaloids; D001600:Berberine Alkaloids; C007232:dehydrocorydalin; C014215:tetrahydropalmatine