title: Endogenous and exogenous ghrelin enhance the colonic and gastric manifestations of dextran sodium sulphate-induced colitis in mice.
abstract: Ghrelin is an important orexigenic peptide that not only exerts gastroprokinetic but also immunoregulatory effects. This study aimed to assess the role of endogenous and exogenous ghrelin in the pathogenesis of colitis and in the disturbances of gastric emptying and colonic contractility during this process. Dextran sodium sulphate colitis was induced for 5 days in (i) ghrelin(+/+) and ghrelin(-/-) mice and clinical and histological parameters were monitored at days 5, 10 and 26 and (ii) in Naval Medical Research Institute non-inbred Swiss (NMRI) mice treated with ghrelin (100 nmol kg(-1)) twice daily for 5 or 10 days. Neural contractility changes were measured in colonic smooth muscle strips, whereas gastric emptying was measured with the (14)C octanoic acid breath test. Inflammation increased ghrelin plasma levels. Body weight loss, histological damage, myeloperoxidase activity and IL-1beta levels were attenuated in ghrelin(-/-) mice. Whereas absence of ghrelin did not affect changes in colonic contractility, gastric emptying in the acute phase was accelerated in ghrelin(+/+) but not in ghrelin(-/-) mice. In agreement with the studies in ghrelin knockout mice, 10 days treatment of NMRI mice with exogenous ghrelin enhanced the clinical disease activity and promoted infiltration of neutrophils and colonic IL-1beta levels. Unexpectedly, ghrelin treatment decreased excitatory and inhibitory neural responses in the colon of healthy but not of inflamed NMRI mice. Endogenous ghrelin enhances the course of the inflammatory process and is involved in the disturbances of gastric emptying associated with colitis. Treatment with exogenous ghrelin aggravates colitis, thereby limiting the potential therapeutic properties of ghrelin during intestinal inflammation.
authors.affiliation: Centre for Gastroenterological Research, Catholic University of Leuven, Leuven, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: De Smet
authors.forename: T
authors.initials: T
authors.lastname: Thijs
authors.forename: D
authors.initials: D
authors.lastname: Moechars
authors.forename: B
authors.initials: B
authors.lastname: Colsoul
authors.forename: L
authors.initials: L
authors.lastname: Polders
authors.forename: L
authors.initials: L
authors.lastname: Ver Donck
authors.forename: B
authors.initials: B
authors.lastname: Coulie
authors.forename: T L
authors.initials: TL
authors.lastname: Peeters
authors.forename: I
authors.initials: I
authors.lastname: Depoortere
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D016264:Dextran Sulfate / Q000633:toxicity; D005746:Gastric Emptying / Q000187:drug effects / Q000502:physiology; D054439:Ghrelin / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology
chemical_list: D054439:Ghrelin; D016264:Dextran Sulfate