Effects of Coenzyme Q10 on TNF-alpha secretion in human and murine monocytic cell lines.
Studies in humans and cell culture as well as bioinformatics suggested that Coenzyme Q(10) (CoQ10) functions as an anti-inflammatory molecule. Here we studied the influence of CoQ10 (Kaneka Q10) on secretion of the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) by using the human and murine monocytic cell lines THP-1 and RAW264.7 expressing human apolipoprotein E3 (apoE3) or pro-inflammatory apoE4. Incubation of cells with physiological (0.1-10 microM) and supra-physiological (> 10 to < 100 microM) concentrations of CoQ10 led to an intracellular accumulation of its reduced form without any cytotoxic effects. Stimulation of cell models with lipopolysaccharide (LPS) resulted in a substantially release of TNF-alpha. When THP-1 cells were pre-incubated with 10 microM CoQ10, the LPS-induced TNF-alpha release was significantly decreased to 72 +/- 32%. This effect is similar to those obtained by 10 microM N-Acetyl-Cysteine, a well known reference antioxidant. In RAW264.7-apoE3 and -apoE4 cells, significant reductions of LPS-induced TNF-alpha secretion to 73.3 +/- 2.8% and 74.7 +/- 8.9% were found with 2.5 microM and 75 microM CoQ10, respectively. In conclusion, CoQ10 has moderate anti-inflammatory effects in two monocytic cell lines which could be mediated by its antioxidant activity.
Schmelzer
Constance
C
Institute of Human Nutrition and Food Science, Molecular Nutrition, Christian-Albrechts-University of Kiel, Heinrich-Hecht-Platz 10, Kiel, Germany. schmelzer@molnut.uni-kiel.de
Lorenz
Gerti
G
Lindner
Inka
I
Rimbach
Gerald
G
Niklowitz
Petra
P
Menke
Thomas
T
Döring
Frank
F
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D053318:Apolipoprotein E3 / Q000502:physiology; D053327:Apolipoprotein E4 / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D053318:Apolipoprotein E3; D053327:Apolipoprotein E4; D014409:Tumor Necrosis Factor-alpha; D014451:Ubiquinone; C024989:coenzyme Q10