Resolution of (R,S)-(+/-)-glycidyl butyrate with immobilized Y-11 Trichosporon capitatum lipase in gelatin-containing microemulsion-based organogels.
An extracellular lipase produced by the strain of Y-11 Trichosporon capitatum was purified to homogeneity by ammonium sulfate precipitation and four chromatographic steps. The purified lipase showed enhanced activity when it was immobilized in gelatin-containing microemulsion-based organogels. Furthermore, the resolution of (R,S)-(+/-)-glycidyl butyrate by this immobilized lipase gave a product of (S)-(-)-glycidol with approximately 98% ee (E=96). By using chiral HPLC separation, (S)-(-)-glycidol was obtained in enantiopure form. Scaled-up reaction in 2l shake flask was also performed and the repeated use of 15 times of immobilized Y-11 T. capitatum lipase resulted in little loss in its activity (4.8%).
Song
Xin
X
State Key Laboratory of Microbial Technology, Shandong University, No. 27 Shanda nan Road, Jinan, Shandong 250100, China. songx@sdu.edu.cn
Qi
Xiaoyu
X
Qu
Yinbo
Y
D002087:Butyrates / Q000302:isolation & purification*; D004655:Emulsions; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds / Q000302:isolation & purification; D005780:Gelatin; D006863:Hydrogen-Ion Concentration; D008049:Lipase; D020005:Propanols / Q000302:isolation & purification; D014250:Trichosporon / Q000201:enzymology*
D002087:Butyrates; D004655:Emulsions; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D020005:Propanols; D005780:Gelatin; D008049:Lipase; C004312:glycidol