Optimal microbial adaptation routes for the rapid degradation of high concentration of phenol.
Pseudomonas fluorescence KNU417 was able to degrade up to 700 mg/L of phenol in 65 h but could not degrade 1,000 mg/L of phenol. Phenol degradation rate was noticeably enhanced by pre-adaptation. In addition, the cell was able to degrade up to 1,300 mg/L of phenol by pre-adapting to 700 mg/L of phenol. Repeated adaptations to the same concentration of phenol showed negligible increase in degradation rate. Also, relatively low concentration of phenol (100-700 mg/L) required only one pre-adaptation while high concentration (1,000 mg/L) did two consecutive stepwise pre-adaptations for rapid degradation. Optimal adaptation routes were suggested for the fast phenol degradation. For example, 1,000 mg/L of phenol was degraded as fast as in 48 h when the cell was pre-adapted to 100 and 300 mg/L of phenol sequentially. The mechanism of adaptation was explained in terms of catechol 1,2-dioxygenase induction, related to aromatic ring cleavage.
Kwon
Kyung Han
KH
Department of Environmental and Applied Chemical Engineering, Kangnung National University, Gangneung, 210-702, South Korea.
Yeom
Sung Ho
SH
D000222:Adaptation, Physiological; D001673:Biodegradation, Environmental; D001698:Biomedical Engineering; D050548:Catechol 1,2-Dioxygenase / Q000096:biosynthesis; D004785:Environmental Pollutants / Q000378:metabolism; D004790:Enzyme Induction; D007700:Kinetics; D019800:Phenol / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism
D004785:Environmental Pollutants; D019800:Phenol; D050548:Catechol 1,2-Dioxygenase