[Effect of electroacupuncture of different acupoints on plasma and cerebral endothelin and CGRP contents in acute cerebral ischemia rats].
OBJECTIVE
To investigate the underlying mechanism of electroacupuncture (EA) of "Shuigou" (GV 26) and "Baihui" (GV 20), "Ganshu" (BL 18) and "Shenshu" (BL 23) in resisting cerebral ischemia (CI).


METHODS
Sixty male SD rats were randomly divided into pseudo-operation group, model group, GV26-GV20 group, and BL18-BL23 group, with 15 cases in each. CI model was established by middle cerebral artery occlusion (MCAO). EA (2/30 Hz,2 mA) was applied to GV26, GV20, BL18 and BL23 for 30 min every time and for 6 times. Neuroethologic scores of the rats were evaluated, plasma and cerebral endothelin (ET) and calcitonin gene-related peptide (CGRP) contents were determined by radioimmunorassay.


RESULTS
1) After MCAO for 72 hours, the rats' neuroethologic score, plasma and cerebral ET contents in model group increased significantly in comparison with those of pseudo-operation group (P<0.05, 0.01), and plasma and cerebral CGRP levels decreased considerably (P<0.01). Compared with model group, the neuroethologic scores, cerebral ET contents of GV26-GV20 and BL18-BL23 groups decreased significantly after CI (P<0.01); and plasma and cerebral CGRP levels in two EA groups increased significantly (P<0.05, 0.01). No significant differences were found between two EA groups in these 3 indexes (P>0.05).


CONCLUSIONS
EA of GV26-GV20 and BL18-BL23 can improve CI rats' behavior activity, which may be related to its effects in lowering cerebral ET and raising blood and cerebral CGRP levels.
Hu
Rong
R
Hunan High Training School of Chinese Medicine,Zhuzhou 412012, China. ronghu780915@126.com
Yan
Jie
J
Li
Tie-Lang
TL
D015669:Acupuncture Points; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism / Q000628:therapy*; D015740:Calcitonin Gene-Related Peptide / Q000097:blood / Q000378:metabolism*; D015671:Electroacupuncture; D016232:Endothelins / Q000097:blood / Q000378:metabolism*; D008297:Male; D010949:Plasma / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D016232:Endothelins; D015740:Calcitonin Gene-Related Peptide