title: Integrin binding immunoglobulin type filamin domains have variable stability.
abstract: Filamin, a large modular protein composed mainly of many immunoglobulin-like domains, is a potent cross-linker of actin filaments. The region containing immunoglobulin type modules 19-21 makes up the binding site for the cytoplasmic tails of the integrin adhesion receptors. Here we investigate the stability of the Ig-like filamin domains using NMR studies over a range of pH and temperature. We show that the 21st Ig-like module (FLNa21) is partly unfolded even under physiological conditions and when attached to FLNa20. It is, however, appreciably stabilized upon binding to integrins. FLNa21 is noticeably less stable than neighboring homologous modules, such as FLNa19 and FLNa17. This variability in stability could be related to the known sensitivity of filamin to cell-mediated mechanical forces.
authors.affiliation: Biochemistry Department, University of Oxford, Oxford, UK.
authors.forename: Pengju
authors.initials: P
authors.lastname: Jiang
authors.forename: Iain D
authors.initials: ID
authors.lastname: Campbell
mesh_terms: D001665:Binding Sites; D003285:Contractile Proteins / Q000737:chemistry* / Q000378:metabolism; D004355:Drug Stability; D064448:Filamins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000737:chemistry / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D008840:Microfilament Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D013816:Thermodynamics
chemical_list: D003285:Contractile Proteins; D064448:Filamins; D007136:Immunoglobulins; D016023:Integrins; D008840:Microfilament Proteins; D010446:Peptide Fragments