Cloning and characterization of alpha-methylacyl coenzyme A racemase from Gordonia polyisoprenivorans VH2.
The mcr gene of Gordonia polyisoprenivorans VH2 is not clustered with genes required for rubber degradation. Its disruption by insertion of a kanamycin resistance cassette impaired growth on methyl-branched isoprenoids but not on linear hydrocarbons. Intact mcr from this bacterium or from Nocardia farcinica IFM 10152 complemented the mutant. Reverse transcription analysis showed similar mcr(VH2) expression results during cultivation with poly(cis-1,4-isoprene) and propionate. Additional genes coding for a putative cytochrome P450 monooxygenase and a short-chain dehydrogenase/reductase involved in beta-oxidation and poly(cis-1,4-isoprene) degradation were also characterized.
Arenskötter
Quyen
Q
Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Correnstrasse 3, D-48149 Münster, Germany.
Heller
Jens
J
Dietz
David
D
Arenskötter
Matthias
M
Steinbüchel
Alexander
A
D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D003065:Coenzyme A / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005816:Genetic Complementation Test; D016254:Mutagenesis, Insertional; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism; D011422:Propionates / Q000378:metabolism; D019998:Racemases and Epimerases / Q000235:genetics* / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism
D005656:Fungal Proteins; D011422:Propionates; D013729:Terpenes; D003577:Cytochrome P-450 Enzyme System; C036543:short chain trans-2-enoyl-CoA reductase; D009247:NADH, NADPH Oxidoreductases; D064429:Fatty Acid Synthases; D019998:Racemases and Epimerases; D003065:Coenzyme A