title: Novel caspase-suicide proteins for tamoxifen-inducible apoptosis.
abstract: Taking advantage of a mutant estrogen receptor ligand binding domain (ER(T2)), we developed novel Caspase fusion proteins for inducible apoptosis. We show that Caspase-ER(T2) fusion proteins become specifically activated by the synthetic ligand 4-OH- tamoxifen and rapidly induce apoptotic cell death in human, murine, and zebrafish cells. This novel tool for targeted cell ablation greatly facilitates the generation of disease models as well as developmental and regeneration studies in model organisms.
authors.affiliation: Helmholtz Zentrum München, German Research Center for Environmental Health, Institute for Developmental Genetics, 85764 Munich/Neuherberg, Germany.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Chu
authors.forename: Niklas
authors.initials: N
authors.lastname: Senghaas
authors.forename: Reinhard W
authors.initials: RW
authors.lastname: Köster
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wurst
authors.forename: Ralf
authors.initials: R
authors.lastname: Kühn
mesh_terms: D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001665:Binding Sites / Q000187:drug effects / Q000235:genetics; D020169:Caspases / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002461:Cell Line, Transformed; D041722:Genes, Transgenic, Suicide / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D020845:Selective Estrogen Receptor Modulators / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology*; D015027:Zebrafish
chemical_list: D018931:Antineoplastic Agents, Hormonal; D020845:Selective Estrogen Receptor Modulators; D013629:Tamoxifen; D020169:Caspases