title: The effects of nanoparticles on mouse testis Leydig cells in vitro.
abstract: We have indicated the possibility that nanoparticles such as diesel exhaust particles (DEP) and titanium dioxide (TiO(2)) may impair the male mouse reproductive system. In this study, to evaluate the direct effect of nanoparticles on testis-constituent cells, we examined the effect of DEP, TiO(2) and carbon black (CB) on mouse Leydig TM3 cells, the testosterone-producing cells of the testis. The uptake of three nanoparticles into Leydig cells was detected using transmission electron microscopy (TEM) or field emission type scanning electron microscopy/energy-dispersive X-ray spectroscopy (FE-SEM/EDS). We examined the cytotoxicity and the effect on gene expression by treatment with nanoparticles. TiO(2) was more cytotoxic to Leydig cells than other nanoparticles. The proliferation of Leydig cells was suppressed transiently by treatment with TiO(2) or DEP. The expression of heme oxygenase-1 (HO-1), a sensitive marker for oxidative stress, was induced remarkably by treatment with DEP. Furthermore, CB and DEP slightly increased the gene expression of the steroidogenic acute regulatory (StAR) protein, the factor that controls mitochondrial cholesterol transfer. In this study, we found that DEPs, TiO(2) and CB nanoparticles were taken up by Leydig cells, and affected the viability, proliferation and gene expression. The patterns were unique for each nanoparticle.
authors.affiliation: Department of Hygiene-Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki Noda-shi, Chiba 278-8510, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Komatsu
authors.forename: Masako
authors.initials: M
authors.lastname: Tabata
authors.forename: Miyoko
authors.initials: M
authors.lastname: Kubo-Irie
authors.forename: Takahisa
authors.initials: T
authors.lastname: Shimizu
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Nihei
authors.forename: Ken
authors.initials: K
authors.lastname: Takeda
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000187:drug effects / Q000378:metabolism; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D053758:Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D010750:Phosphoproteins / Q000187:drug effects / Q000378:metabolism; D053260:Soot / Q000633:toxicity*; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D014025:Titanium / Q000633:toxicity*; D018675:Toxicity Tests; D001335:Vehicle Emissions / Q000633:toxicity*; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D010750:Phosphoproteins; D053260:Soot; D001335:Vehicle Emissions; D000098682:Steroidogenic Acute Regulatory Protein; C009495:titanium dioxide; D014025:Titanium; D051547:Heme Oxygenase-1