title: Genetic divergence among marine and lagoon Atherina boyeri populations in Greece using mtDNA analysis.
abstract: Genetic differentiation and phylogenetic relationships among 15 Atherina boyeri populations from several marine and lagoon or lake sites in Greece were investigated using mtDNA analysis. PCR-RFLP analysis of 12s, 16s rRNA genes and D-loop revealed 23 haplotypes. All the lake or lagoon populations, as well as the Kymi and Kalymnos populations that originated from sites with lagoonlike environmental conditions, showed haplotypes 1-6, clearly distinguishable from the marine populations, which exhibited types 7-23. The genetic divergence values estimated between the lagoon and the marine populations ranged from 5.55 to 10.45%. The high genetic differentiation observed between these two types of populations is also highlighted by the dendrograms obtained using UPGMA and maximum parsimony methods.
authors.affiliation: Division of Genetics, Cell Biology and Development, Department of Biology, University of Patras, Patras, 26500, Greece.
authors.forename: Spiridoula
authors.initials: S
authors.lastname: Kraitsek
authors.forename: Elena
authors.initials: E
authors.lastname: Klossa-Kilia
authors.forename: Vasilis
authors.initials: V
authors.lastname: Papasotiropoulos
authors.forename: Stamatis N
authors.initials: SN
authors.lastname: Alahiotis
authors.forename: George
authors.initials: G
authors.lastname: Kilias
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry*; D014644:Genetic Variation; D005828:Genetics, Population; D006115:Greece; D006239:Haplotypes; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D023701:Smegmamorpha / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal