Regulation of expression of Sertoli cell glucose transporters 1 and 3 by FSH, IL1 beta, and bFGF at two different time-points in pubertal development.
Sertoli cells are necessary to provide adequate levels of lactate for germ cell development. Lactate production is hormonally regulated by follicle-stimulating hormone (FSH) and by a large set of intratesticular regulators such as interleukin-1 beta (IL1 beta) and basic fibroblast growth factor (bFGF). Little is known regarding the critical step in the production of this metabolite, viz., the entrance of glucose into the cell as mediated by GLUTs. The aim of the present study was to investigate the expression of the glucose transporters GLUT1 and GLUT3 and its possible regulation by FSH, IL1 beta, and bFGF in Sertoli cells at two different time-points in sexual development. Sertoli cells retaining the ability to undergo mitosis (obtained from 8-day-old rats) and in the process of terminal differentiation (obtained from 20-day-old rats) were examined. Testicular tissue sections and Sertoli cell monolayers obtained from 8- and 20-day-old rats showed positive immunostaining for GLUT1 and GLUT3 proteins. GLUT1 and GLUT3 mRNA levels were detected at the two ages analyzed. Treatment of Sertoli cells obtained from 8- and 20-day-old rats with FSH, IL1 beta, and bFGF for various periods of time (12, 24, and 48 h) increased GLUT1 without changing GLUT3 mRNA levels. Our results thus show that Sertoli cells express GLUT1 and GLUT3 throughout pubertal development, and that, in Sertoli cells, only GLUT1 is regulated by hormones during pubertal development. Hormonal regulation of GLUT1 expression and consequently glucose uptake and lactate production may be a key molecular event in the regulation of spermatogenesis by hormones.
Galardo
María Noel
MN
Centro de Investigaciones Endocrinológicas (CEDIE-CONICET), Hospital de Niños Ricardo Gutiérrez, Gallo 1330, C1425EDF, Buenos Aires, Argentina.
Riera
María Fernanda
MF
Pellizzari
Eliana Herminia
EH
Chemes
Héctor Edgardo
HE
Venara
Marcela Cristina
MC
Cigorraga
Selva Beatriz
SB
Meroni
Silvina Beatriz
SB
D000818:Animals; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology / Q000502:physiology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology / Q000502:physiology; D051272:Glucose Transporter Type 1 / Q000096:biosynthesis* / Q000187:drug effects; D051274:Glucose Transporter Type 3 / Q000096:biosynthesis* / Q000187:drug effects; D053583:Interleukin-1beta / Q000494:pharmacology / Q000502:physiology; D019344:Lactic Acid / Q000096:biosynthesis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D053583:Interleukin-1beta; D016222:Fibroblast Growth Factor 2; D019344:Lactic Acid; D005640:Follicle Stimulating Hormone