title: Evaluation of adhesion, proliferation, and functional differentiation of dermal fibroblasts on glycosaminoglycan-coated polysulfone membranes.
abstract: It may be hypothesized that wound healing will benefit from polymer membranes coated with extracellular matrix macromolecules. Here we describe the behavior of dermal fibroblasts on polysulfone (PSU) membranes, and PSU membranes covered with chitosan (Ch), chondroitin sulfate (CS), or hyaluronan, using an additional intermediary ionic charge modification with poly-(acrylonitrile-co-methallyl sulfonate) (AN69), which allows binding of the polysaccharidic macromolecules. Cell adhesion, proliferation, cell signaling, and collagen gene expression were investigated. Ch and CS were found unable to support cell adhesion and proliferation. In contrast, both PSU and hyaluronic acid-coated PSU membranes appeared as suitable materials to culture fibroblasts and support their matrix synthesis capacity. Moreover, they induce type III collagen expression in addition to type I, suggesting that they promote a fetal-like environment that could be beneficial for wound healing.
authors.affiliation: Laboratory of Extracellular Matrix and Pathology, "IFR 146 ICORE" Faculty of Medicine, University of Caen Basse Normandie, Caen Cedex, France.
authors.forename: Joan
authors.initials: J
authors.lastname: Attia
authors.forename: Florence
authors.initials: F
authors.lastname: Legendre
authors.forename: Quang Trong
authors.initials: QT
authors.lastname: Nguyen
authors.forename: Catherine
authors.initials: C
authors.lastname: Baugé
authors.forename: Karim
authors.initials: K
authors.lastname: Boumediene
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Pujol
mesh_terms: D015153:Blotting, Western; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D024043:Collagen Type II / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D006025:Glycosaminoglycans / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011108:Polymers; D012867:Skin / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D013450:Sulfones; D046509:Tissue Culture Techniques / Q000295:instrumentation / Q000379:methods
chemical_list: D024043:Collagen Type II; D006025:Glycosaminoglycans; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700