Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis.
BACKGROUND
MicroRNAs (miRNAs) are small (approximately 22 nucleotides) regulatory RNAs which play fundamental roles in many human diseases, including cancer. There is no report on the miRNA expression profile of retinoblastoma.


METHODS
This work was undertaken to identify differentially expressed miRNAs in human retinoblastoma tissues by microRNA microarray technique, and some miRNAs were verified using northern blot analysis and the in situ hybridization method.


RESULTS
A cluster of microRNAs was identified as highly expressed in retinoblastoma, including hsa-miR-494, hsa-let-7e, hsa-miR-513-1, hsa-miR-513-2, hsa-miR-518c*, hsa-miR-129-1, hsa-miR-129-2, hsa-miR-198, hsa-miR-492, hsa-miR-498, hsa-miR-320, hsa-miR-503, and hsa-miR-373*.


CONCLUSIONS
These miRNAs are the first to be reported for human retinoblastoma and may play significant roles in regulating tumor genesis.
Zhao
Jian-Jun
JJ
Peking Union Medical College, Tsinghua University, Beijing, China.
Yang
Juhua
J
Lin
Jianhong
J
Yao
Nan
N
Zhu
Yihua
Y
Zheng
Jianlong
J
Xu
Jianhua
J
Cheng
Jin Q
JQ
Lin
Jian-Yin
JY
Ma
Xu
X
D000328:Adult; D015152:Blotting, Northern; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012175:Retinoblastoma / Q000235:genetics*
C527114:MIRN373 microRNA, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human