Optimization of fluorophore-assisted carbohydrate electrophoresis.
Fluorophore-assisted carbohydrate electrophoresis (FACE) is a simple and inexpensive method for separating saccharides. Oligosaccharides were tagged with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulfonate (ANTS), and the reductive amination reactions were essentially complete after approximately 16 h under the given experimental conditions. Saccharide-ANTS adducts were then separated by electrophoresis on 32% C(ACR)/2.4% C(BIS) polyacrylamide gel at alkaline pH. This technique doesn't require sophisticated instrumentation and highly trained personnel.
Huang
Gang L
GL
College of Chemistry, Chongqing Normal University, Chongqing 400047, China. huangdoctor226@163.com
D002241:Carbohydrates / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006868:Hydrolysis
D002241:Carbohydrates; D005456:Fluorescent Dyes