A microfluidic flow injection system for DNA assay with fluids driven by an on-chip integrated pump based on capillary and evaporation effects.
A miniaturized flow injection analysis (FIA) system integrating a micropump on a microfluidic chip based on capillary and evaporation effects was developed. The pump was made by fixing a filter paper plug with a vent tube at the channel end, it requires no peripheral equipment and provides steady flow in the microl min(-1) range for FIA operation. Valve-free sample injection was achieved at nanolitre level using an array of slotted vials. The practical applicability of the system was demonstrated by DNA assay with laser-induced fluorescence (LIF) detection. A precision of 1.6% RSD (10.0 ng microl(-1), n=15) was achieved with a sampling throughput of 76 h(-1) and sample consumption of 95 nl.
Xu
Zhang-Run
ZR
Research Center for Analytical Sciences, Northeastern University, Shenyang, 110004, China.
Zhong
Chong-Hui
CH
Guan
Yan-Xia
YX
Chen
Xu-Wei
XW
Wang
Jian-Hua
JH
Fang
Zhao-Lun
ZL
D004247:DNA / Q000032:analysis*; D017022:Flow Injection Analysis / Q000295:instrumentation*; D044085:Microfluidics / Q000295:instrumentation*; D008904:Miniaturization; D015203:Reproducibility of Results
D004247:DNA