title: Genetic diversity in the C-terminal 42 kDa region of merozoite surface protein-1 of Plasmodium vivax (PvMSP-1(42)) among Indian isolates.
abstract: Plasmodium vivax merozoite surface protein 1 (PvMSP-1) is a leading malaria vaccine candidate. This protein is processed to give rise to various sized fragments during merozoite maturation. Here, we describe the analysis of genetic diversity in the 42 kDa C-terminal part of this protein among 33 Indian P. vivax isolates. A total of 27 haplotypes with 72 mutations and 0.0212+/-0.0005S.D. over all pi nucleotide diversity were observed among the isolates. Twenty-six of 27 haplotypes reported here were new as they have not been reported so far from any other country. The difference between non-synonymous (dN) and synonymous (dS) mutations was found to be positive (0.0081+/-0.0051) for the entire 42 kDa region. Further analysis revealed that 33 kDa (MSP-1(33)) fragment of the MSP-1(42) was highly polymorphic with pi nucleotide diversity 0.0290+/-0.0007S.D. The dN-dS for this region of MSP-1 was also positive (0.0114+/-0.0071S.E.). On the other hand, there was no non-synonymous mutation in the 19 kDa (MSP-1(19)) fragment of the MSP-1(42) and thus it was highly conserved. In conclusion, MSP-1(33) fragment was highly polymorphic and appeared to be under diversifying selection whereas there was no selection at MSP-1(19) region among the isolates. Present study will be helpful for the development of PvMSP-1 based vaccine against P. vivax malaria.
authors.affiliation: Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India.
authors.forename: Ankur
authors.initials: A
authors.lastname: Thakur
authors.forename: Mohammad Tauqeer
authors.initials: MT
authors.lastname: Alam
authors.forename: Yagya D
authors.initials: YD
authors.lastname: Sharma
mesh_terms: D000818:Animals; D006239:Haplotypes; D006801:Humans; D007194:India; D016780:Malaria, Vivax / Q000469:parasitology*; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D020125:Mutation, Missense; D010966:Plasmodium vivax / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic; D012641:Selection, Genetic
chemical_list: D020066:Merozoite Surface Protein 1