Vero cells infected with vaccinia viruses expressing Japanese encephalitis virus envelope protein induce polykaryocyte formation under neutral conditions.
Flavivirus membranes fuse with cellular membranes by low pH-induced mechanisms. Although Japanese encephalitis virus (JEV) has similar mechanisms, fusion induced under neutral conditions has been observed. We report herein polykaryocyte formation using Vero cells infected with recombinant vaccinia viruses, vP829 expressing JEV premembrane (prM) and envelope (E), or vP555 expressing prM, E, and the nonstructural proteins NS1 and NS2A. Polykaryocytes were detected under pH 7.0 as early as 9 h after infection. Higher fusion indices were shown with vP555 than vP829. A monoclonal antibody to E suppressed vP829/vP555-induced polykaryocyte formation. Polykaryocytes were also formed under alkaline conditions (pH 8.0).
Konishi
Eiji
E
Department of International Health, Kobe University Graduate School of Health Sciences, Kobe 654-0142, Japan. ekon@kobe-u.ac.jp
Yagawa
Kyoko
K
Yamanaka
Atsushi
A
D000818:Animals; D002459:Cell Fusion; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D004664:Encephalitis Virus, Japanese / Q000378:metabolism / Q000472:pathogenicity*; D015726:Giant Cells / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008561:Membrane Fusion; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
D014759:Viral Envelope Proteins; C074932:prM protein, Flavivirus