title: Albinism in the American mink (Neovison vison) is associated with a tyrosinase nonsense mutation.
abstract: Albino phenotypes are documented in various species including the American mink. In other species the albino phenotypes are associated with tyrosinase (TYR) gene mutations; therefore TYR was considered the candidate gene for albinism in mink. Four microsatellite markers were chosen in the predicted region of the TYR gene. Genotypes at the markers Mvi6025 and Mvi6034 were found to be associated with the albino phenotype within an extended half-sib family. A BAC clone containing Mvi6034 was mapped to chromosome 7q1.1-q1.3 by fluorescent in situ hybridization. Subsequent analysis of genomic TYR sequences from wild-type and albino mink samples identified a nonsense mutation in exon 1, which converts a TGT codon encoding cysteine to a TGA stop codon (c.138T>A, p.C46X; EU627590). The mutation truncates more than 90% of the normal gene product including the putative catalytic domains. The results indicate that the nonsense mutation is responsible for the albino phenotype in the American mink.
authors.affiliation: Division of Animal Genetics and Bioinformatics, Department of Basic Animal and Veterinary Sciences, The Faculty of Life Sciences, University of Copenhagen, Groennegaardsvej 3, DK-1870 Frederiksberg C, Denmark. ran@life.ku.dk
authors.forename: R
authors.initials: R
authors.lastname: Anistoroaei
authors.forename: M
authors.initials: M
authors.lastname: Fredholm
authors.forename: K
authors.initials: K
authors.lastname: Christensen
authors.forename: T
authors.initials: T
authors.lastname: Leeb
mesh_terms: D000417:Albinism / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial; D018389:Codon, Nonsense; D017931:DNA Primers; D005260:Female; D005838:Genotype; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008907:Mink / Q000235:genetics*; D014442:Monophenol Monooxygenase / Q000235:genetics*; D010375:Pedigree; D016133:Polymerase Chain Reaction
chemical_list: D018389:Codon, Nonsense; D017931:DNA Primers; D014442:Monophenol Monooxygenase