title: Gene expression and MR diffusion-weighted imaging after chemoembolization in rabbit liver VX-2 tumor model.
abstract: OBJECTIVE
  To investigate the dynamic characteristics and the correlation between PCNA, Bax, nm23, E-cadherin expression and apparent diffusion coefficient (ADC) on MR diffusion-weighted imaging (DWI) after chemoembolization in rabbit liver VX-2 tumor model.
  
  
  METHODS
  Forty New Zealand rabbit liver VX-2 tumor models were included in the study. DWI was carried out periodically after chemoembolization. All VX-2 tumor samples in each group were examined by histopathology and Strept Avidin-Biotin Complex (SABC) immunohistochemical staining.
  
  
  RESULTS
  The PCNA expression index in VX-2 tumors was higher than in the normal parenchyma around the tumor (P < 0.001). Nm23, Bax or E-caderin expression index in VX-2 tumors were lower than in the normal parenchyma around the tumor (all P < 0.001). PCNA and nm23 expression in the VX-2 tumor periphery first increased and then decreased (P < 0.001 and P = 0.03, respectively), while the expression of Bax and E-cadherin before and after chemoembolization was insignificant. When b-value was 100 s/mm(2), there was a linear correlation between PCNA expression and ADC in the area of VX-2 tumor periphery (P < 0.001), and PCNA expression in VX-2 tumor periphery influenced the ADC.
  
  
  CONCLUSIONS
  The potential of VX-2 tumor infiltrating and metastasizing decreases, while its ability to proliferate increases for a short time after chemoembolization. To some degree, the ADC value indirectly reflects the proliferation of VX-2 tumor cells.
authors.affiliation: Department of Radiology, Hunan Province People's Hospital, Changsha, Hunan Province, China. heyuanyouhong@yahoo.com.cn
authors.forename: You-Hong
authors.initials: YH
authors.lastname: Yuan
authors.forename: En-Hua
authors.initials: EH
authors.lastname: Xiao
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Liu
authors.forename: Zhong
authors.initials: Z
authors.lastname: He
authors.forename: Ke
authors.initials: K
authors.lastname: Jin
authors.forename: Cong
authors.initials: C
authors.lastname: Ma
authors.forename: Jun
authors.initials: J
authors.lastname: Xiang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Xiao
authors.forename: Wei-Jian
authors.initials: WJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D016461:Chemoembolization, Therapeutic; D038524:Diffusion Magnetic Resonance Imaging; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D015820:Cadherins; D054778:NM23 Nucleoside Diphosphate Kinases; D018809:Proliferating Cell Nuclear Antigen; D051028:bcl-2-Associated X Protein