A rapid serological assay for prediction of Salmonella infection status in slaughter pigs using surface plasmon resonance.
We present a rapid surface plasmon resonance-based serological assay for the detection of Salmonella Typhimurium infection in pigs using the Plasmonic((R)) SPR device. Lipopolysaccharide (LPS, 10 microg mL(-1)) from Salmonella Typhimurium was immobilised by self-assembly on a hydrophobic SPR chip. Using this LPS-coated chip, it was possible to bind and detect the anti-Salmonella Typhimurium antibodies in serum of pigs infected with the bacteria. The developed SPR assay is able to differentiate between sera obtained from pigs having low, medium, and high levels of Salmonella infection. A commercial ELISA kit was used to classify the sera for levels of Salmonella infection on the basis of optical density (OD%). A strong positive correlation was observed between the SPR-based assay and the ELISA (n=38, r=0.90, p<0.01). The sensitivity and specificity of the assay are 0.93 and 0.87, respectively. The SPR-based assay is label-free and does not require any sample preparation or dilution steps. The total analysis time is 45 min for each serum sample. The assay was found to be specific for Salmonella Typhimurium and shows no cross-reactivity to Salmonella Choleraesuis or Escherichia coli antibodies. As no sample preparation is required the developed assay has the potential to be used as a reliable tool for Salmonella monitoring programmes in pork production.
Mazumdar
Saikat Datta
SD
Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Marbacher Weg 6, Marburg, Germany.
Barlen
Benjamin
B
Kramer
Thomas
T
Keusgen
Michael
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology; D012698:Serologic Tests / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000382:microbiology
D000907:Antibodies, Bacterial