title: Extractive spectrophotometric determination of TRODAT-1 hydrochloride in lyophilized kit.
abstract: A simple, sensitive, and accurate spectrophotometric method has been developed for the assay of TRODAT-1 hydrochloride in lyophilized kit. The method is based on the formation of ion-pair association complex of TRODAT-1 with bromothymol blue (BTB) in disodium hydrogen phosphate/citric acid buffer of pH 4.0. The colored product was extracted with chloroform, and measured spectrophotometrically at 414 nm. Beer's law was obeyed in the range of 5-25 microg/ml with molar absorptivity of 2.75 x 10(4) l/mol/cm. Optimization of experimental conditions was described for the method. The proposed method has been successfully applied for the analysis of TRODAT-1 hydrochloride in lyophilized kit. No interference with pharmaceutical excipients was observed.
authors.affiliation: Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, P.R. China.
authors.forename: X M
authors.initials: XM
authors.lastname: Li
authors.forename: Z P
authors.initials: ZP
authors.lastname: Chen
authors.forename: S P
authors.initials: SP
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Tang
authors.forename: C Y
authors.initials: CY
authors.lastname: Liu
authors.forename: M F
authors.initials: MF
authors.lastname: Zou
mesh_terms: D001979:Bromthymol Blue; D004396:Coloring Agents; D005612:Freeze Drying; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015609:Organotechnetium Compounds / Q000032:analysis*; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet; D014326:Tropanes / Q000032:analysis*
chemical_list: D004396:Coloring Agents; D007202:Indicators and Reagents; D015609:Organotechnetium Compounds; D014326:Tropanes; C104196:technetium Tc 99m TRODAT-1; D001979:Bromthymol Blue