title: Brugmansia suaveolens mottle virus, a novel potyvirus causing leaf mottling of Brugmansia suaveolens in Brazil.
abstract: A potyvirus was isolated from Brugmansia suaveolens showing leaf mottling and tentatively named Brugmansia suaveolens mottle virus (BsMoV). The virus (isolate Bs-Campinas) could infect some solanaceous plants and two Chenopodium species, and was transmitted by aphids. Symptomatic leaves contained flexuous particles and cylindrical inclusions. RT-PCR amplification using potyvirus universal primers produced a DNA fragment of 1851 nt (3' terminal genomic region), which shared 71% nucleotide identity with Pepper mottle virus, the best-matched potyvirus sequence. Since this identity value is below the threshold currently used to discriminate Potyvirus species, Brugmansia suaveolens mottle virus most likely represents a new Potyvirus species.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Católica de Brasília, Brasília, DF, Brazil.
authors.forename: N
authors.initials: N
authors.lastname: Lucinda
authors.forename: T
authors.initials: T
authors.lastname: Nagata
authors.forename: A K
authors.initials: AK
authors.lastname: Inoue-Nagata
authors.forename: R B
authors.initials: RB
authors.lastname: Salaroli
authors.forename: E W
authors.initials: EW
authors.lastname: Kitajima
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology; D001938:Brazil; D004199:Disease Vectors; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D017800:Potyvirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D019657:Solanaceae / Q000821:virology*
chemical_list: D012367:RNA, Viral