[Optimization of the supercritical fluid extraction process of Tuirezhitongsan].
OBJECTIVE
To optimize the extraction process of Tuirezhitongsan and extract the pharmacodynamically active fractions.


METHODS
Orthogonal test methods and supercritical-CO(2) fluid extraction (SFE-CO(2)) were used for the extraction, and the the pharmacodynamically active fractions were screened with analgesic experiments.


RESULTS
The optimal extraction of the active fractions was achieved with the extraction pressure at 25 MPa, extraction temperature at 45 degrees celsius, resolution pressure at 6.7 MPa, and resolution temperature at 50 degrees celsius.


CONCLUSIONS
SFE- CO(2) is rapid, efficient, and energy-saving in the extraction of the active fractions in Tuirezhotongsan, and provides a mew method for extraction of traditional Chinese medicine.
Zhou
Ben-jie
BJ
Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou, China. zhoubj163@163.com
Huang
Qing-wei
QW
Feng
Li-juan
LJ
D019342:Acetic Acid; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005260:Female; D008297:Male; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; C539568:tuirezhitongsan; D019342:Acetic Acid