Bioluminescence imaging of hepatitis B virus enhancer and promoter activities in mice.
By bioluminescence imaging and hydrodynamic gene transfer technology, the activities of hepatitis B virus (HBV) promoters and the effects of HBV enhancers on these promoters in mice under true physiological conditions have been assessed. Our studies reveal that either of the two HBV enhancers can stimulate HBV major promoter activity in hepa 1-6 cells (in vitro) and in mouse liver (in vivo), and the enhancer effects on the three promoters (S1, S2 and X promoter) are markedly greater in vivo than in vitro. The two HBV enhancers have no cooperative action on HBV promoters in vitro or in vivo.
Du
Juan
J
Laboratory of Blood-borne Virus, Beijing Institute of Transfusion Medicine, Tai Ping Road 27, Beijing 100850, China.
Zhou
Yong
Y
Fu
Qiu-Xia
QX
Gong
Wei-Li
WL
Zhao
Fang
F
Peng
Jian-Chun
JC
Zhan
Lin-Sheng
LS
D000818:Animals; D045744:Cell Line, Tumor; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006515:Hepatitis B virus / Q000235:genetics*; D008099:Liver / Q000378:metabolism / Q000821:virology*; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011401:Promoter Regions, Genetic
D008156:Luciferases