[Effect of polysaccharides in crude and processed Cornus officinalis on the immunologic function of mice with immunosuppression induced].
OBJECTIVE
To investigate the effect of polysaccharides in crude and processed Cornus officinalis on the immunologic function of mice with immunosuppression induced.


METHODS
The immunosuppressed mice were induced by Cyclophosphamide. Non-specific immune function was determined by cleaning carbon particle method. Humoral immunity was determined by serum haemolysin formation method. Cellular immunity was determined by proliferation and transformation of spleen lymphocyte method.


RESULTS
The polysaccharides in crude and processed Cornus officinalis both markedly increased the carbon particle clearance index K, phagocytic index alpha, serum HC50 and proliferation and transformation of spleen lymphocyte,and the polysaccharides in processed Cornus officinalis was better than the crude one.


CONCLUSIONS
The polysaccharides in crude and processed Cornus officinalis have an enhanced effect on non-specific immunity, specific humoral immunity and specific cellular immunity in immunodeppressed mice, and after being processed with wine, the action of polysaccharides increased markedly.
Du
Wei-Feng
WF
Jiangsu key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nangjing 210029, China. duweifeng200158@sohu.com
Wang
Ming-Yan
MY
Cai
Bao-Chang
BC
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D029768:Cornus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005638:Fruit / Q000737:chemistry; D006168:Guinea Pigs; D007109:Immunity / Q000187:drug effects*; D007165:Immunosuppression Therapy; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013678:Technology, Pharmaceutical
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides