Expression and polimorphism of IFN-gamma gene in patients with cervical cancer.
BACKGROUND
Cervical cancer, the second most common malignancy in women worldwide, is almost invariably associated with infection by human papillomavirus (HPV). However, although many women are infected with high-risk types of HPV, only a subset of infected women will ever develop cervical cancer. Several studies suggested that immunological components play a key role in the development of cervical cancer. Interferon gamma (IFN-gamma ) is a cytokine produced by activated T cells and natural killer (NK) cells that enhances cellular immune responses by increasing T-cell cytotoxicity and NK cell activity.


OBJECTIVE
To study single nucleotide polymorphism (SNP), T to A, located at the +874 position and measure IFN-gamma messenger RNA (mRNA) at the tumor site.


METHODS
DNA was isolated from peripheral blood of 200 patients with cervical cancer and 200 healthy controls. The allele polymorphism at position +874 in the IFN-gamma gene was studied by ARMS-PCR (Amplification Refractory Mutation System) and measured IFN-gamma mRNA at the tumor site by means of a semi-quantitative polymerase chain reaction (sqRT-PCR) assay.


RESULTS
It was observed that genotypes AT and AA + AT increase the risk of cervical cancer (OR = 3.3, 95% CI - 2.05-5.2, P <or= 0.001 - OR = 2.9, 95% CI - 1.9-4.6, P <or= 0.001, respectively). In case of passive smokers same genotypes showed highly significant increased risk of cervical cancer (OR = 5.55, 95% CI = 2.77-11.19 - OR = 5.25, 95% CI = 2.77-10, respectively). Thus, the sqRT-PCR reflected the similar level of mRNA expression of IFN- gamma gene in patients suffering from cervical carcinoma and healthy controls.


CONCLUSIONS
This is the first study to provide an evidence for effecting of IFN-gamma gene on the risk of cervical cancer in north Indian population.
Kordi Tamandani
M K
MK
Department of Biotechnology, Panjab University, Chandigrah, India. dor_kordi@yahoo.com
Sobti
R C
RC
Shekari
M
M
Mukesh
M
M
Suri
V
V
D000230:Adenocarcinoma / Q000235:genetics / Q000556:secondary; D000293:Adolescent; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000556:secondary; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D007371:Interferon-gamma