title: DNA sequencing-based typing of HPA-1 to HPA-17w systems.
abstract: Although several DNA-based human platelet antigens (HPA) typing techniques, such as PCR-SSP and PCR-SSO, have been established, the typing errors and the lack of interlaboratory reproducibility are still the issues of concerns. In the present study, polymerase chain reaction primers were designed for identification of all the phenotypically different HPA-1 to HPA-17w types by sequencing-based typing (SBT) method using genomic DNA samples. No discrepancies were observed between PCR-SSP typing and SBT typing in typing a panel of HPA-typed platelet donors that included all common HPA types and the rare HPA-1b, 2b, 3b, and 6bw homozygous donors.
authors.affiliation: Nan-Ning Institute of Transfusion Medicine, 18 Keyuan Avenue, 530003, Nanning, China. guangwu@szonline.net.
authors.forename: Guo-Guang
authors.initials: GG
authors.lastname: Wu
authors.affiliation: Nan-Ning Institute of Transfusion Medicine, 18 Keyuan Avenue, 530003, Nanning, China.
authors.forename: Qiu-Ming
authors.initials: QM
authors.lastname: Tang
authors.affiliation: Nan-Ning Institute of Transfusion Medicine, 18 Keyuan Avenue, 530003, Nanning, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Shen
authors.affiliation: Nan-Ning Institute of Transfusion Medicine, 18 Keyuan Avenue, 530003, Nanning, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Nan-Ning Institute of Transfusion Medicine, 18 Keyuan Avenue, 530003, Nanning, China.
authors.forename: Heng-Cong
authors.initials: HC
authors.lastname: Li
authors.affiliation: National Institutes of Health, Bethesda, USA.
authors.forename: Tong-Mao
authors.initials: TM
authors.lastname: Zhao
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics*; D005260:Female; D006801:Humans; D019520:Living Donors; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D016824:Antigens, Human Platelet