title: Antitumor effect of adriamycin-encapsulated nanoparticles of poly(DL-lactide-co-glycolide)-grafted dextran.
abstract: In this study, we prepared adriamycin (ADR)-encapsulated core-shell type nanoparticles of a poly(DL-lactide-co-glycolide) (PLGA) grafted-dextran (DexLG) copolymer and evaluated its antitumor activity in vitro and in vivo. The particle size of ADR-encapsulated DexLG nanoparticles was around 50-200 nm and the morphology was spherical shapes at transmission electron microscopy (TEM) observation. Since reconstitution of lyophilized nanoparticles is essential to practical use in vivo, ADR-encapsulated DexLG nanoparticles were lyophilized and reconstituted them into deionized water. Although reconstitution process caused increase of particle size, drug release behavior of nanoparticles was not significantly changed before and after reconstitution process. The ADR-encapsulated DexLG nanoparticles were less cytotoxic than free ADR plus empty nanoparticles at in vitro, while empty DexLG nanoparticles did not significantly affect cell viability. Even if free ADR plus empty nanoparticles are most effective to inhibit tumor growth at tumor-induced animal model using CT-26 cells, ADR-encapsulated DexLG nanoparticles showed increased survivability of mice. These results indicated that ADR-encapsulated DexLG nanoparticles are promising vehicles for antitumor drug delivery.
authors.affiliation: Department of Pet Science, Seojeong College, Gyeonggi-do 482-777, South Korea.
authors.forename: Ki-Choon
authors.initials: KC
authors.lastname: Choi
authors.forename: Je-Yong
authors.initials: JY
authors.lastname: Bang
authors.forename: Chan
authors.initials: C
authors.lastname: Kim
authors.forename: Pyoung-Il
authors.initials: PI
authors.lastname: Kim
authors.forename: Sang-Rak
authors.initials: SR
authors.lastname: Lee
authors.forename: Wan-Tae
authors.initials: WT
authors.lastname: Chung
authors.forename: Woo-Dae
authors.initials: WD
authors.lastname: Park
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Yeon Soo
authors.initials: YS
authors.lastname: Lee
authors.forename: Chae-Eun
authors.initials: CE
authors.lastname: Song
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Lee
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000627:therapeutic use / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D003911:Dextrans; D004317:Doxorubicin / Q000008:administration & dosage* / Q000627:therapeutic use / Q000633:toxicity; D004337:Drug Carriers; D005612:Freeze Drying; D006801:Humans; D019344:Lactic Acid; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles; D010316:Particle Size; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000903:Antibiotics, Antineoplastic; D003911:Dextrans; D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004317:Doxorubicin