title: The transcriptional terminator sequences downstream of the covR gene terminate covR/S operon transcription to generate covR monocistronic transcripts in Streptococcus pyogenes.
abstract: CovR/S is an important two component regulatory system, which regulates about 15% of the gene expression in Streptococcus pyogenes. The covR/S locus was identified as an operon generating an RNA transcript around 2.5-kb in size. In this study, we found the covR/S operon produced three RNA transcripts (around 2.5-, 1.0-, and 0.8-kb in size). Using RNA transcriptional terminator sequence prediction and transcriptional terminator analysis, we identified two atypical rho-independent terminator sequences downstream of the covR gene and showed these terminator sequences terminate RNA transcription efficiently. These results indicate that covR/S operon generates covR/S transcript and monocistronic covR transcripts.
authors.affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chuan
authors.initials: C
authors.lastname: Chiang-Ni
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Tsou
authors.forename: Yee-Shin
authors.initials: YS
authors.lastname: Lin
authors.forename: Woei-Jer
authors.initials: WJ
authors.lastname: Chuang
authors.forename: Ming-T
authors.initials: MT
authors.lastname: Lin
authors.forename: Ching-Chuan
authors.initials: CC
authors.lastname: Liu
authors.forename: Jiunn-Jong
authors.initials: JJ
authors.lastname: Wu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D005798:Genes, Bacterial; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D010957:Plasmids / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics*; D013297:Streptococcus pyogenes / Q000235:genetics*; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C432143:CsrR protein, Streptococcus pyogenes; D012097:Repressor Proteins; D012313:RNA