Comparison of 3T3 fibroblasts growth on alginate and polyasparagine (PAA) scaffolds in mouse model.
BACKGROUND
The aim of this study was to evaluate polyasparagine (PAA), a new, promising scaffold. PAA was compared with alginate, which is used in cell transplantations and may be regarded as a standard.


METHODS
In vitro cell viability on both scaffolds was assessed. C57B1 mice were injected s. c. alginate or PAA with or without 3T3 cells. After two months specimens from sites of injection were examined and blood samples were taken for enzymatic activity estimation. Cathepsin D activity and alpha1-antitrypsin levels were measured.


RESULTS
In vitro cell viability was lowest on PAA and highest in control group. Increase in levels of enzymes measured was observed in response to PAA and alginate and was lower in case of polymer seeded with cells. Increase in alpha1-antitrypsin levels was lower in case of PAA in comparison to alginate. Scaffold degradation in histopathological specimens was visible.


CONCLUSIONS
The results indicate that PAA implants undergo biodegradation and nonspecific inflammatory response is comparable to alginate.
Drewa
Tomasz
T
Department of Tissue Engineering, Chair of Medical Biology, Nicolaus Copernicus University, Bydgoszcz, Poland. tomaszdrewa@wp.pl
Jasiński
Miłosz
M
Polaczek
Jolanta
J
Pielichowski
Jan
J
Kierzenkowska-Mila
Celestyna
C
Łysik-Miśkurska
Joanna
J
Krawczyk
Agnieszka
A
D016475:3T3 Cells / Q000378:metabolism / Q000473:pathology; D000464:Alginates; D000818:Animals; D002402:Cathepsin D / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides; D054457:Tissue Scaffolds; D014357:Trypsin / Q000378:metabolism
D000464:Alginates; D006603:Hexuronic Acids; D010455:Peptides; C045164:polyasparagine; D020723:Glucuronic Acid; D014357:Trypsin; D002402:Cathepsin D