[Calixarene-dependent hydrolysis of ATP. I. Kinetics and complexation of the calixarene C-107 with nucleoside triphosphate].
It was shown that calix[4]arene bis-aminomethylphosphonic acid C-107 can hydrolyze ATP. The kinetic curve of the ATP hydrolysis induced by calixarene C-107 was nonhyperbolic and had a tendency to plateau (in the course of time) observing from 45-60 minutes of the incubation period when the reaction practically came to the end. The empirical kinetic characteristics of this reaction were calculated. The velocity of calixarene-dependent hydrolysis of ATP exceeds the velocity of spontaneous hydrolysis of ATP at least 14-15 times. The "Host-Guest" complexation of the calixarene C-107 with adenosinetriphosphate in acetonitrile/water (47/53 v/v) solution was investigated by the reversed-phase high performance liquid chromatography. The dissociation constants of the 1:1 "Host-Guest" complexes ofATP-Guest with the calixarene-Host when using two columns Zorbax CN and LiChrosorb RP 18 within 197-231 microM were determined from the capacity factor of the Guest and concentration of the calixarene-Host in the mobile phase. The electrostatic, ion-dipole, dipole-dipole C-H-pi and other weak interactions in the "Host-Guest" complexes were discussed. Obtained data can be a basis for designing the synthetical ATP-hydrolyzing catalysts and also for subsequent investigation of both enzymatic and nonenzymatic ATP hydrolysis reaction,processes of ATP-dependent Ca2+ -transporting in subcellular membrane structures.
Shkrabak
O A
OA
Kal'chenko
O I
OI
Rodik
R V
RV
Veklich
T O
TO
Kal'chenko
V I
VI
Kosterin
S O
SO
D000255:Adenosine Triphosphate / Q000737:chemistry*; D047250:Calixarenes / Q000138:chemical synthesis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D007700:Kinetics; D008958:Models, Molecular; D015394:Molecular Structure
C533334:calixarene C-107; D047250:Calixarenes; D000255:Adenosine Triphosphate