Serum levels of osteopontin are increased in SIRS and sepsis.
OBJECTIVE
In sepsis, dysregulation of the immune response leads to rapid multiorgan failure and death. Accurate and timely diagnosis is lifesaving and should discriminate sepsis from the systemic inflammatory response syndrome (SIRS) caused by non-infectious agents. Osteopontin acts as an extracellular matrix component or a soluble cytokine in inflamed tissues. Its exact role in immune response and sepsis remains to be elucidated. Therefore, we investigated the role of osteopontin in SIRS and sepsis.


METHODS
Prospective, observational study.


METHODS
Intensive care unit of a university hospital.


METHODS
Fifty-six patients with SIRS or sepsis and 56 healthy subjects were enrolled.


METHODS
We analyzed the serum levels of osteopontin and TH1-TH2 cytokines and investigated the role of osteopontin on interleukin 6 secretion by monocytes.


RESULTS
Serum osteopontin levels were strikingly higher in patients than in controls and in sepsis than in SIRS, and decreased during the resolution of both the disorders. Receiver operating characteristic curves showed that osteopontin levels have discriminative power between SIRS and sepsis with an area under the curve of 0.796. Osteopontin levels directly correlated with those of interleukin 6 and in vitro, recombinant osteopontin increased interleukin 6 secretion by monocytes in both the absence and presence of high doses of lipopolysaccharide.


CONCLUSIONS
These data suggest that osteopontin might be a mediator involved in the pathogenesis of SIRS and sepsis, possibly by supporting interleukin 6 secretion.


BACKGROUND
45. SIRS/Sepsis: clinical studies.
Vaschetto
Rosanna
R
Department of Clinical and Experimental Medicine, SCDU Anaesthesia and Intensive Care 1, Maggiore della Carità Hospital, "A. Avogadro" University of Eastern Piedmont, Novara, Italy.
Nicola
Stefania
S
Olivieri
Carlo
C
Boggio
Elena
E
Piccolella
Fabio
F
Mesturini
Riccardo
R
Damnotti
Federica
F
Colombo
Davide
D
Navalesi
Paolo
P
Della Corte
Francesco
F
Dianzani
Umberto
U
Chiocchetti
Annalisa
A
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007362:Intensive Care Units; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000097:blood*; D011446:Prospective Studies; D012372:ROC Curve; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood* / Q000175:diagnosis
D015415:Biomarkers; D015850:Interleukin-6; D053495:Osteopontin