[Influence of different penetration enhancers on Lappaconitine transcutaneous permeation].
OBJECTIVE
To investigate the effects of different kinds and concentration of transdermal enhancers on Lappaconitine transcutaneous permeation when used individually or together.


METHODS
Using modified Franz-type diffusion cell and excised human body skin as an in vitro transdermal model, the concentration of lappaconitine was determined by HPLC, then cumulative permeation quantity (Q) and stability rate (J) of progesterone were calculated.


RESULTS
Penetration enhancers such as propylene glycol, dodecanol, IPM, and particularly 3% OA and Azone, can significantly enhance the penetration rate of lappaconitine. Concentration effect of penetration enhancers concentration on lappaconitine transcutaneous permeation were found in experiments, the permeation effect of Azone was better than Azone + OA and Azone + propylene glycol.


CONCLUSIONS
The transdermal rate of lappaconitine from batch which contains 3% OA or Azone is higher than others. Combination of Azone with other penetration enhancers is not recommended for Lappaconitine transcutaneous permeation.
Han
Min
M
Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, China.
Zhong
Yun-Wei
YW
Li
Xin-Ping
XP
Wu
Zheng
Z
Liang
Wen-Quan
WQ
Gao
Jian-Qing
JQ
D000157:Aconitine / Q000031:analogs & derivatives* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000378:metabolism; D006801:Humans; D010539:Permeability / Q000187:drug effects; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D000157:Aconitine; C022150:lappaconitine