title: Negative association between plasma levels of adiponectin and polychlorinated biphenyl 153 in obese women under non-energy-restrictive regime.
abstract: The aim of this study was to reveal whether accumulation of the persistent organic pollutants (POPs), especially polychlorinated biphenyl (2,2',4,4',5,5'-hexachlorobiphenyl, PCB 153), affects plasma levels of adiponectin in obese patients. The study was designed as a longitudinal intervention trial with a control group, where 27 obese women (body mass index (BMI)>30 kg/m(2); age 21-74 years) were studied before (OB) and after (OB-LCD) a 3-month low-calorie-diet intervention (LCD; 5 MJ daily). As the control group, 9 female volunteers without LCD intervention were used (C; BMI=19-25 kg/m(2); age 21-64 years). Plasma levels of PCB 153 were measured by high-resolution gas chromatography with electron capture detection; total adiponectin and insulin plasma levels were quantified by immunoassays; and adiponectin multimeric complexes were quantified by immunoblotting. Plasma levels of total adiponectin, high and medium molecular weight multimers significantly negatively correlated with plasma levels of PCB 153 in OB, but not in C or in OB-LCD, whereas the LCD intervention lowered BMI by 3.3+/-3.0 kg/m(2). Our results may suggest suppression of adiponectin by PCB 153 in obese women under non-energy-restrictive regime, which may contribute to the known association of PCB 153 and other POPs with type 2 diabetes.
authors.affiliation: 1st Medical Department, Charles University Medical School and Teaching Hospital at Plzen, Plzen, Czech Republic. dana.mullerova@lfp.cuni.cz
authors.forename: D
authors.initials: D
authors.lastname: Mullerova
authors.forename: J
authors.initials: J
authors.lastname: Kopecky
authors.forename: D
authors.initials: D
authors.lastname: Matejkova
authors.forename: L
authors.initials: L
authors.lastname: Muller
authors.forename: J
authors.initials: J
authors.lastname: Rosmus
authors.forename: J
authors.initials: J
authors.lastname: Racek
authors.forename: F
authors.initials: F
authors.lastname: Sefrna
authors.forename: S
authors.initials: S
authors.lastname: Opatrna
authors.forename: O
authors.initials: O
authors.lastname: Kuda
authors.forename: M
authors.initials: M
authors.lastname: Matejovic
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D031204:Caloric Restriction; D016022:Case-Control Studies; D018153:Czech Republic; D004785:Environmental Pollutants / Q000097:blood*; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D008137:Longitudinal Studies; D008875:Middle Aged; D009765:Obesity / Q000097:blood*; D011078:Polychlorinated Biphenyls / Q000097:blood*; D055815:Young Adult
chemical_list: D052242:Adiponectin; D015415:Biomarkers; D004785:Environmental Pollutants; D007328:Insulin; D011078:Polychlorinated Biphenyls; C014024:2,4,5,2',4',5'-hexachlorobiphenyl