Development of protein delivery microsphere system by a novel S/O/O/W multi-emulsion.
A novel method has been developed to protect protein drugs in poly (lactic-co-glycolic acid) (PLGA) microspheres using S/O/O/W multi-emulsion method. This method develops a novel protein drug sustained-release system, which is based on the combination of protein-loaded dextran glassy microparticles (protein-loaded AqueSpheres) and PLGA microspheres. The protein molecules are encapsulated in the dextran glassy particles and the drug-containing dextran glassy particles are further dispersed in the PLGA microspheres. The protein-loaded AqueSpheres based PLGA composite microspheres have spherical shape and a smooth surface. They possess a normal size distribution and a mean diameter of 67.08 microm. The method may decrease protein aggregations and incomplete release due to avoiding protein contacting with oil/water interfaces and hydrophobic PLGA. The dextran glassy particles can stabilize proteins in the PLGA matrix, which is the major advantage of this novel protein sustained-release system over preparation for the PLGA microspheres using W/O/W double-emulsion method.
Yuan
Weien
W
Shanghai Jiaotong University, School of Pharmacy, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
Wu
Fei
F
Guo
Meiyan
M
Jin
Tuo
T
D000818:Animals; D001682:Biological Availability; D002417:Cattle; D002850:Chromatography, Gel; D003692:Delayed-Action Preparations / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D004655:Emulsions; D005990:Glycerol / Q000737:chemistry; D006736:Horses; D019344:Lactic Acid / Q000737:chemistry; D008752:Methylene Chloride / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009211:Myoglobin / Q000008:administration & dosage / Q000737:chemistry; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011142:Polyvinyl Alcohol / Q000737:chemistry; D019946:Propylene Glycol / Q000737:chemistry; D011506:Proteins / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000493:pharmacokinetics; D013499:Surface Properties
D003692:Delayed-Action Preparations; D003911:Dextrans; D004655:Emulsions; D009211:Myoglobin; D011506:Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D008752:Methylene Chloride; D019946:Propylene Glycol; D011142:Polyvinyl Alcohol; D005990:Glycerol