[Anti-allergic effects of Rubus suavissimus extract].
OBJECTIVE
To investigate the effects of Rubus suavissimus S. Lee extract (RSE) on mice, and its influence on release of histamine from rat peritoneal mast cells induced by compound 48/80 in vitro.


METHODS
To establish murine delayed-type-hypersensitive model induced by 2,4-dinitrofluotobenzene (DNFB) and sheep red blood cell (SRBC); murine type I allergic reaction induced by PCA, and then to observe the anti-allergic effect of RSE. HPLC-ECD methods were performed to detect the contents of histamine released from rat peritoneal mast cells induced by compound 48/80 in vitro.


RESULTS
Compared with the model group, RSE could ameliorate the ear swelling and capillary permeability in mice induced by DNFB and PCA. RSE also reduced the thickness of the mice hind paw induced by SRBC in significant dose-dependent manner. Meanwhile, RSE obviously inhibited the release of histamine in rat peritoneal mast in vitro, a dose-dependent manner.


CONCLUSIONS
RSE exerts potential anti-allergy effects and the mechanisms may be partly related to its inhibitory effect on the release of histamine from mast cells.
Fang
Yao-Gao
YG
Dongguan Houjie People's Hospital, Dongguan 511771, China.
Lu
Hui-Wen
HW
Feng
Jin-He
JH
Bao
Li
L
Kurihara
Hiroshi
H
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004139:Dinitrofluorobenzene; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D006636:Histamine Release / Q000187:drug effects; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D051379:Mice; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D027824:Rosaceae / Q000737:chemistry*
D018926:Anti-Allergic Agents; D004365:Drugs, Chinese Herbal; D004139:Dinitrofluorobenzene