title: Fatty acid profile of human milk of Portuguese lactating women: prospective study from the 1st to the 16th week of lactation.
abstract: OBJECTIVE
  Fatty acid (FA) composition varies over the course of the day and during lactation. The aim of this study was to evaluate FA composition and its compositional stability in human milk, from day 7 to week 16 of lactation.
  
  
  METHODS
  Human milk was collected from all feedings over 24 h at day 7 and weeks 4, 8, 12 and 16 of lactation in 31 lactating women. FAs were analyzed through gas chromatography. Comparisons were made with analysis of variance.
  
  
  RESULTS
  Total monounsaturated FAs decreased from 33.04 +/- 2.58% wt/wt at day 7 to 31.48 +/- 3.32% wt/wt at week 16 of lactation, much at the expenses of the decrease in the major monounsaturated FA found in human milk, oleic acid. Main polyunsaturated FAs n-6 and n-3 showed fluctuations from day 7 up to week 16 of lactation, but with no statistical significance. Arachidonic acid significantly decreased from transitional to mature milk.
  
  
  CONCLUSIONS
  The FA profile obtained throughout the study time points presented very low levels of oleic acid and very high linoleic acid/alpha-linoleic acid ratios which reflect recent changes in Portuguese women's food patterns. Despite this, the arachidonic acid/docosahexaenoic acid ratio [corrected] remained constant during the study, suggesting a protective metabolic mechanism.
authors.affiliation: Department of Paediatrics, Hospital S. João, Faculty of Medicine, Porto, Portugal. mrtribeiro@gmail.com
authors.forename: M
authors.initials: M
authors.lastname: Ribeiro
authors.forename: V
authors.initials: V
authors.lastname: Balcao
authors.forename: H
authors.initials: H
authors.lastname: Guimaraes
authors.forename: G
authors.initials: G
authors.lastname: Rocha
authors.forename: C
authors.initials: C
authors.lastname: Moutinho
authors.forename: C
authors.initials: C
authors.lastname: Matos
authors.forename: C
authors.initials: C
authors.lastname: Almeida
authors.forename: S
authors.initials: S
authors.lastname: Casal
authors.forename: A
authors.initials: A
authors.lastname: Guerra
mesh_terms: D000328:Adult; D001205:Ascorbic Acid / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D003683:Dehydroascorbic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D007774:Lactation / Q000378:metabolism*; D008895:Milk, Human / Q000737:chemistry*; D049590:Postpartum Period; D012016:Reference Values; D013997:Time Factors; D055815:Young Adult
chemical_list: D005227:Fatty Acids; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid