Attenuation of mitochondrial injury by L-arginine preconditioning of the liver.
The present study was aimed to evaluate the efficacy of L-arginine on mitochondrial function in ischemic and reperfusion (I/R) induced hepatic injury. Adult Wistar rat were subjected to 1 h of partial liver ischemia followed by 3 hour reperfusion. Eighteen wistar rats were divided into three groups viz. sham-operated control group (I) (n=6), ischemia and reperfusion (I/R) group (II) (n=6), L-arginine treated group (100 mg/kg body weight/daily by oral route for 7 days before induced ischemia reperfusion maneuver) (III) (n=6). Mitochondrial injury was assessed in terms of decreased (P<0.05) activities of mitochondrial antioxidant enzymes (GSH, SOD, CAT), respiratory marker enzymes (NADH dehydrogenase, cytochrome c oxidases) and hepatocytes nitric oxide production. Pre-treatment with L-arginine (10 mg/kg/p.o. for 7 days) significantly counteracted the alternations of hepatic enzymes and mitochondrial respiratory and antioxidant enzymes. In addition, electron microscopy and histopathology study showed the restoration of cellular normalcy and accredits the cytoprotective role of L-arginine against I/R induced hepatocellular injury. On the basis of these findings it may be concluded that L-arginine protects mitochondrial function in hepatic ischemic and reperfused liver.
Chattopadhyay
Pronobesh
P
Cellular and Microbiology Laboratory, College of Pharmacy, IFTM, Moradabad, U.P., India. chatto_pronobesh@rediffmail.com
Shukla
Gunjan
G
Verma
Anurag
A
Wahi
A K
AK
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001120:Arginine / Q000627:therapeutic use*; D003576:Electron Transport Complex IV / Q000378:metabolism; D048550:Hepatic Insufficiency / Q000188:drug therapy*; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000648:ultrastructure; D009245:NADH Dehydrogenase / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D013385:Succinate Dehydrogenase / Q000378:metabolism
D000975:Antioxidants; D009569:Nitric Oxide; D001120:Arginine; D008291:Malate Dehydrogenase; D007655:Ketoglutarate Dehydrogenase Complex; D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV