title: [Effect of porcine relaxin on NO production of human microvascular endothelial cells].
abstract: OBJECTIVE
  To observe effect of porcine relaxin(pRLX) on NO production of human microvascular endothelial cells(HMVECs) and discuss its possible mechanism.
  
  
  METHODS
  iNOS and cNOS expression of HMVECs with or without pRLX were detected using western blotting. NO production of HMVECs with pRLX at different concentration or different time were determined by method of Griess. NO production of pRLX of HMVECs plus Non-selective NOS inhibitor NG-monomethyl-L-arginine(L-NMMA), selective iNOS inhibitor aminoguanidine(AG) or nuclear factors-kappaB (NF-kappaB) inhibitor pyrrolidine dithiocarbamate(PDTC) were also analysed.
  
  
  RESULTS
  pRLX promoted iNOS protein expression of HMVECs, but not cNOS protein expression. NO production of HMVECs was promoted by pRLX on concentration-dependent pattern instead of time-dependent one. AG, L-NMMA and PDTC were showed to block the effect of pRLX on NO production of HMVECs.
  
  
  CONCLUSIONS
  pRLX promote iNOS expression and NO production of HMVECs.
authors.affiliation: Department of Cardiology, Liuhuaqiao Hospital, Guangzhou, China. furuibin@21cn.com
authors.forename: Rui-bin
authors.initials: RB
authors.lastname: Fu
authors.forename: Jian
authors.initials: J
authors.lastname: Qiu
authors.forename: Ding-cheng
authors.initials: DC
authors.lastname: Xiang
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: He
authors.forename: Chang-jiang
authors.initials: CJ
authors.lastname: Hong
authors.forename: Jin-xia
authors.initials: JX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000098:blood supply; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D012065:Relaxin / Q000494:pharmacology*; D013552:Swine; D013997:Time Factors
chemical_list: D009569:Nitric Oxide; D012065:Relaxin; D052247:Nitric Oxide Synthase Type II