title: Phenotypic and genetic characterisation of methicillin-resistant Staphylococcus aureus strains isolated from the university hospitals of Debrecen.
abstract: The purpose of this study was to characterise methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 2005 at the university hospitals of Debrecen, Hungary. Three hundred and thirty-nine MRSA strains were isolated from 102 patients at 18 different clinics. Their sensitivity to oxacillin and ten other antibiotics was determined. For genotypic analysis, phage typing and pulsed-field gel electrophoresis (PFGE) was performed. The rate of MRSA strains increased to 7.2% in 2005, especially at the clinics of surgery, pulmonology and paediatrics. No vancomycin- or teicoplanin-resistant strains were found. The resistance to erythromycin, clindamycin and ciprofloxacin was nearly 100% and multi-resistance was very frequent. Fifty-eight percent of the isolates belonged to mixed phage types and 8% was non-typable. One PFGE clone contained 58.2% of all strains and two further major clones were found at a separately located clinical block, indicating intra-hospital spread. We can conclude that MRSA exhibits an increasing nosocomial problem also in Hungary.
authors.affiliation: Department of Medical Microbiology, Medical and Health Centre, University of Debrecen, P. O. B. 17, 4012, Debrecen, Hungary. szabjud@dote.hu
authors.forename: J
authors.initials: J
authors.lastname: Szabó
authors.forename: Z
authors.initials: Z
authors.lastname: Dombrádi
authors.forename: O
authors.initials: O
authors.lastname: Dobay
authors.forename: P
authors.initials: P
authors.lastname: Orosi
authors.forename: J
authors.initials: J
authors.lastname: Kónya
authors.forename: K
authors.initials: K
authors.lastname: Nagy
authors.forename: F
authors.initials: F
authors.lastname: Rozgonyi
mesh_terms: D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D006785:Hospitals, University; D006801:Humans; D006814:Hungary; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D015995:Prevalence; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology