Collagen-binding motif peptide, a cleavage product of osteopontin, stimulates human neutrophil chemotaxis via pertussis toxin-sensitive G protein-mediated signaling.
The collagen-binding motif (CBM) peptide, a cleavage product of osteopontin (OPN), stimulated intracellular calcium increase in human neutrophils. CBM peptide-stimulated calcium was inhibited by pertussis toxin (PTX), suggesting the influence of PTX-sensitive G-proteins. In addition CBM peptide stimulated the chemotactic migration of human neutrophils and human monocytes. CBM peptide-induced neutrophil chemotaxis was completely inhibited by PTX, once again indicating the influence of Gi proteins. CBM peptide was also found to induce mitogen activated protein kinase activation. CBM peptide-induced neutrophil chemotaxis was mediated by p38 kinase as well as an extracellular signal-regulated protein kinase. Taken together, the results suggest that a cleavage product of OPN, CBM peptide, initiates immune responses by inducing neutrophil trafficking via certain PTX-sensitive cell surface receptors.
Kim
Mi-Kyoung
MK
Department of Biochemistry, College of Medicine, Dong-A University, 3-1 Dongdaesindong Seogu, Busan 602-714, Republic of Korea.
Kim
Sang Doo
SD
Lee
Ha Young
HY
Lee
Sun Young
SY
Shim
Jae Woong
JW
Yun
Jeanho
J
Kim
Jong-Min
JM
Min
Do Sik
do S
Yoo
Young Hyun
YH
Bae
Yoe-Sik
YS
D020816:Amino Acid Motifs; D002478:Cells, Cultured; D002633:Chemotaxis; D003094:Collagen / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D053495:Osteopontin / Q000378:metabolism / Q000494:pharmacology*; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology*; D037342:Pertussis Toxin / Q000494:pharmacology
D010446:Peptide Fragments; D053495:Osteopontin; D003094:Collagen; D037342:Pertussis Toxin; D048049:Extracellular Signal-Regulated MAP Kinases; D019204:GTP-Binding Proteins