The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media.
OBJECTIVE
This study was to investigate the effect of sodium selenite (SS) on in vitro maturation of mouse preantral follicles.


METHODS
The isolated preantral follicles were cultured in TCM 199 medium supplemented with different concentrations (0, 5, 10, 15 ng/ml) of SS and 3 mg/ml bovine serum albumin (BSA) or 5% Fetal Bovine Serum (FBS). The ovulation was induced by addition of 1.5 IU/ml human chorionic gonadotropin. The size and development of follicles and oocytes were assessed by calibrated eyepiece.


RESULTS
The survival rates of follicles in FBS supplemented groups containing 5 and 10 ng/ml SS (88.23%, 90.83%) were higher than other groups (P < 0.05 and P < 0.001 respectively). The mean diameter of follicles (199.84 +/- 15.58 microm) and the percentage of MII oocyte (33.08%) were higher in FBS supplemented group containing 10 ng/ml SS (P < 0.001).


CONCLUSIONS
The sodium selenite and FBS improve the in vitro growth and maturation of mouse preantral follicles.
Abedelahi
A
A
Department of Anatomy, Tarbiat Modares University, P. O. BOX: 14115-111, Tehran, Iran.
Salehnia
M
M
Allameh
A A
AA
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D016895:Culture Media, Serum-Free; D005260:Female; D006801:Humans; D051379:Mice; D006080:Ovarian Follicle / Q000166:cytology* / Q000187:drug effects; D010060:Ovulation; D011994:Recombinant Proteins / Q000494:pharmacology; D044967:Serum; D018038:Sodium Selenite / Q000494:pharmacology*; D046509:Tissue Culture Techniques
D006063:Chorionic Gonadotropin; D016895:Culture Media, Serum-Free; D011994:Recombinant Proteins; D018038:Sodium Selenite