title: Determination of bergenin in human plasma after oral administration by HPLC-MS/MS method and its pharmacokinetic study.
abstract: A highly sensitive, simple and selective high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and applied to the determination of bergenin concentration in human plasma. Bergenin and the internal standard (IS) thiamphenicol in plasma were extracted with ethyl acetate, separated on a C(18 )reversed-phase column, eluted with mobile phase of acetonitrile-water, ionized by negative ion pneumatically assisted electrospray and detected in the multi-reaction monitoring mode using precursor --> product ions of m/z 327.1 --> 192 for bergenin and 354 --> 185.1 for the IS, respectively. The linear range of the calibration curve for bergenin was 0.25-60 ng mL(-1), with the lowest limit of quantification of 0.25 ng mL(-1), and the intra/inter-day relative standard deviation (RSD) was less than 10%. The method is suitable for the determination of low bergenin concentration in human plasma after therapeutic oral doses, and has been first and successfully used for its pharmacokinetic studies in healthy Chinese volunteers.
authors.affiliation: Institute of Pharmacology, Medicine College of Shandong University, Jinan 250012, the People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Ben-Jie
authors.initials: BJ
authors.lastname: Wang
authors.forename: Chun-Min
authors.initials: CM
authors.lastname: Wei
authors.forename: Gui-Yan
authors.initials: GY
authors.lastname: Yuan
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Rui-Chen
authors.initials: RC
authors.lastname: Guo
mesh_terms: D000284:Administration, Oral; D001578:Benzopyrans / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D016014:Linear Models; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013839:Thiamphenicol / Q000032:analysis
chemical_list: D001578:Benzopyrans; D013839:Thiamphenicol; C006741:bergenin