Discrimination analysis of mass spectrometry proteomics for ovarian cancer detection.
OBJECTIVE
A discrimination analysis has been explored for the probabilistic classification of healthy versus ovarian cancer serum samples using proteomics data from mass spectrometry (MS).


METHODS
The method employs data normalization, clustering, and a linear discriminant analysis on surface-enhanced laser desorption ionization (SELDI) time-of-flight MS data. The probabilistic classification method computes the optimal linear discriminant using the complex human blood serum SELDI spectra. Cross-validation and training/testing data-split experiments are conducted to verify the optimal discriminant and demonstrate the accuracy and robustness of the method.


RESULTS
The cluster discrimination method achieves excellent performance. The sensitivity, specificity, and positive predictive values are above 97% on ovarian cancer. The protein fraction peaks, which significantly contribute to the classification, can be available from the analysis process.


CONCLUSIONS
The discrimination analysis helps the molecular identities of differentially expressed proteins and peptides between the healthy and ovarian patients.
Hong
Yan-jun
YJ
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Wang
Xiao-dan
XD
Shen
David
D
Zeng
Su
S
D000465:Algorithms; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics*; D040901:Proteomics; D012016:Reference Values; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009363:Neoplasm Proteins