Expression analysis of the exo-beta-1,3-glucanase from the mycoparasitic fungus Trichoderma asperellum.
The regulation of the gene encoding the extracellular exo-beta-1,3-glucanase (tag83) produced by the mycoparasite Trichoderma asperellum was studied. Enzyme activity was detected in all carbon sources, but the highest levels were found when starch and purified cell walls from Rhizoctonia solani were used. These results are supported by the appearance of one strong band with enzyme activity in non-denaturing PAGE. Experiments using RT-PCR showed that exo-beta-1,3-glucanase induction in T. asperellum occurred at the transcriptional level. We used RT-PCR and real-time PCR analysis to examine the expression of tag83 gene during in vivo assay of T. asperellum against R. solani. We showed that the expression of tag83 is significantly increased by the presence of R. solani.
Marcello
César Marcos
CM
Departamento de Biologia Celular, Universidade de Brasília, 70910-900 DF, Brazil.
Steindorff
Andrei Stecca
AS
da Silva
Silvana Petrofeza
SP
Silva
Roberto do Nascimento
Rdo N
Mendes Bataus
Luiz Artur
LA
Ulhoa
Cirano José
CJ
D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics* / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
D005656:Fungal Proteins; D043326:Glucan 1,3-beta-Glucosidase