Control of the blue fluorescent protein with advanced evolutionary pulse shaping.
We demonstrate optical coherent control of the two-photon fluorescence of the blue fluorescent protein (BFP), which is of interest in investigations of protein-protein interactions. In addition to biological relevance, BFP represents an interesting target for coherent control from a chemical perspective due to its many components of highly nonexponential fluorescence decay and low quantum yield resulting from excited state isomerization. Using a genetic algorithm with a multiplicative (rather than ratiometric) fitness parameter, we are able to control the ratio of BFP fluorescence to second-harmonic generation without a considerable drop in the maximized signal. The importance of linear chirp and power-scaling on the discrimination process is investigated in detail.
Tkaczyk
Eric R
ER
Center for Ultrafast Optical Science, University of Michigan, 2200 Bonisteel Boulevard, Ann Arbor, MI 48109-2099, USA. etkaczyk@umich.edu
Mauring
Koit
K
Tkaczyk
Alan H
AH
Krasnenko
Veera
V
Ye
Jing Yong
JY
Baker
James R
JR
Norris
Theodore B
TB
D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics; D017785:Photons
D008164:Luminescent Proteins