[Studies on fingerprints of Bupleurum chinense and vinegar-baked Bupleurum chinense from Hubei province].
OBJECTIVE
To establish the HPLC Fingerprint of Bupleurum chinense from GAP provenance of Hubei province Baokang, and discuss the changes before and after processing.


METHODS
Using HPLC detection method equipped with Hypersil ODS column (5 microm, 4.6 mm x 250 mm), using acetonitrile-water as mobile phase gradient elution, flow as 1.0 ml/min, UV detector set at 203 nm, column temperature was at 25 degrees C.


RESULTS
Similarity of processed Bupleurum chinense and Bupleurum chinense exceeded 0.95, and there were 13 characteristic fingerprints of processed Bupleurum chinense, 15 characteristic fingerprints of Bupleurum chinense.


CONCLUSIONS
The HPLC Fingerprint of Bupleurum chinense from GAP provenance of Hubei province Baokang are established, and the difference between Bupleurum chinense before and after processing is studied initially.
Nie
Sa
S
College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China. niesa163@163.com
Liu
Xiao-Ping
XP
Chen
Shao-Wen
SW
Li
Kai
K
D029972:Bupleurum / Q000737:chemistry* / Q000254:growth & development; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011208:Powders; D011786:Quality Control; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013678:Technology, Pharmaceutical / Q000379:methods
D004365:Drugs, Chinese Herbal; D011208:Powders