title: Novel SNPs of the bovine LEPR gene and their association with growth traits.
abstract: In this study, polymorphism in the bovine LEPR gene exon 4 was detected by PCR-SSCP and DNA sequencing methods in 653 individuals from five Chinese cattle breeds. Two haplotypes (M and N), three observed genotypes (MM, MN, and NN), and five single nucleotide polymorphisms (SNPs) (NC_007301:g.26767T>C, NC_007301:g.26805C>T, NC_007301:g.27050A>G, NC_007301:g.27063G>A, NC_007301:g.27079G>A) were detected. The frequencies of haplotypes M and N in the five breeds were 0.661-0.747 and 0.253-0.339, respectively. The SNP locus was in Hardy-Weinberg equilibrium in Nanyang, Jiaxian red, Angus, and Jinnan cattle (P > 0.05) and was in Hardy-Weinberg disequilibrium in Qinchuan cattle (P < 0.05). Polymorphism of the LEPR gene was shown to be associated with growth traits in the Nanyang breed. The SNP in the bovine LEPR gene had significant effects on body height, body length, body weight, heart girth, and average daily gain at 6 and 12 months old (P < 0.01 or P < 0.05). Therefore, these results suggest that the LEPR gene is a strong candidate gene that affects growth traits in cattle.
authors.affiliation: College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, PR China.
authors.forename: Yikun
authors.initials: Y
authors.lastname: Guo
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Chuanying
authors.initials: C
authors.lastname: Pan
authors.forename: Liangzhi
authors.initials: L
authors.lastname: Zhang
authors.forename: Cunfang
authors.initials: C
authors.lastname: Zhang
authors.forename: Miao
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001483:Base Sequence; D001823:Body Composition / Q000235:genetics; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D005091:Exons; D006239:Haplotypes; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D054411:Receptors, Leptin / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D054411:Receptors, Leptin