title: Simple enrichment and independent cultures to expand bacterial community analysis from Gedongsongo hot spring.
abstract: Denaturing gradient gel electrophoresis was used to identify bacterial communities from Gedongsongo hot spring, Indonesia, by culture-independent and -dependent procedures. Predominant species were found to be closely related to beta-proteobacteria, particularly from genus Ralstonia and Delftia. Other species present include alpha- and gamma-proteobacteria and Thermus groups. Some of the strains showed unique 16S rDNA sequences.
authors.affiliation: Biochemistry Research Division, Faculty of Mathematics and Natural Science, Institut Teknologi Bandung, Bandung, Indonesia.
authors.forename: Agustina Lulustyaningati Nurul
authors.initials: AL
authors.lastname: Aminin
authors.forename: Fida Madayanti
authors.initials: FM
authors.lastname: Warganegara
authors.forename: Pingkan
authors.initials: P
authors.lastname: Aditiawati
authors.lastname: Akhmaloka
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D045482:Hot Springs; D007214:Indonesia; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial