Association of DRB1 and DQB1 HLA class II polymorphisms in high-grade and neoplastic cervical lesions of women from Argentina.
OBJECTIVE
To investigate associations between HLA-DRB1 and HLA-DQB1 polymorphisms with low- and high-grade cervical lesions in Argentine population and the role human papillomavirus status in these associations.


METHODS
Cervical biopsies and peripheral blood samples were taken from 32 patients with cervical intraepithelial neoplasia grade 1 (CIN 1) and 44 patients with cervical CIN 3 or invasive squamous cell carcinomas. Cervical cells and peripheral blood samples from 40 healthy women were included as control group. Human papillomavirus detection and typing were done by polymerase chain reaction (PCR) MY09, 11-restriction fragment length polymorphisms, or PCR 5+, 6+ dot-blot hybridization, and HLA DR/DQ typing by the PCR-sequence-specific oligonucleotide probes method.


RESULTS
HLA-DRB1*04 and HLA-DQB1*0302 were found to be positive associated with the CIN 3/invasive squamous cell carcinomas subgroup, whereas HLA-DRB1*13 and HLA-DQB1*02 were negatively associated with the same group, when comparing to the control group.


CONCLUSIONS
The data support the hypothesis that HLA-DRB1*04 and HLA-DQB1*0302 may be considered risk factors for malignant progression, whereas HLA-DRB1*13 and HLA-DQB1*02 may have a protective role. Further studies with a larger group are needed to confirm these susceptibility and protective roles in disease progression in Argentine population.
Eiguchi
Kumiko
K
Cátedra de Bioquímica e Inmunología, Facultad de Medicina, Universidad del Salvador, Buenos Aires, Argentina. keiguchi@salvador.edu.ar
Tatti
Silvio
S
Alonio
L Virginia
LV
González
Joaquín V
JV
Leirós
Gustavo J
GJ
Fleider
Laura
L
Vighi
Susana
S
Padros
Karin
K
Raimondi
Eduardo
E
Teyssié
Angélica
A
Picconi
M Alejandra
MA
D000328:Adult; D000483:Alleles; D001118:Argentina; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002584:Cervix Uteri / Q000821:virology; D018450:Disease Progression; D005260:Female; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000235:genetics; D011110:Polymorphism, Genetic; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology; D044465:White People / Q000235:genetics; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000821:virology
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains