title: The rapid identification of isoflavonoids from Belamcanda chinensis by LC-NMR and LC-MS.
abstract: The use of hyphenated LC-NMR and LC-MS techniques for the purpose of directly identifying the major constituents of Belamcanda chinensis was investigated. Reversed-phase isocratic chromatography was performed using an acetonitrile-water solvent system on a C18 column. The NMR spectrum yielded five main peaks, whose analysis revealed them to be 5, 6, 7, 3'-tetrahydroxy-4'-methoxyisoflavone (1), tectorigenin (2), iristectorigenin A (3), irigenin (4), and irisflorentine (5). The identification of these constituents was confirmed by performing LC-ESI-MS experiment. This study shows that hyphenated LC-NMR and LC-MS can be used for the rapid (70 min) identification of the isoflavonoids.
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Korea.
authors.forename: Suk Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Min Cheol
authors.initials: MC
authors.lastname: Kim
authors.forename: Chul Young
authors.initials: CY
authors.lastname: Kim
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Jung
authors.forename: Byung Hun
authors.initials: BH
authors.lastname: Um
mesh_terms: D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D027621:Iridaceae / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D018517:Plant Roots / Q000737:chemistry; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007529:Isoflavones; D012997:Solvents; D000431:Ethanol