[DNA polymorphism detection of Papaver somniferum L using fluorescent amplified fragment length polymorphism].
OBJECTIVE
To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.


METHODS
Genomic DNA was isolated using the AxyPrep DNA Kit, double-digested by two restrictional endonucleases (Eco RI and Mse I) and ligated to oligonucleotide adapters. After Pre-amplification and selective amplification, the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.


RESULTS
More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.


CONCLUSIONS
The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.
Lu
Fan
F
Institute of Criminal Science and Technology, Yunnan Province Police Bureau, Kunming 650021, China.
Cheng
Bao-wen
BW
Li
Hong
H
Hong
Jun-jie
JJ
Sun
Hai-Yan
HY
Zhao
Wen-song
WS
Yang
Hong-mei
HM
Li
Jin
J
Zhao
Yu-rong
YR
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D005456:Fluorescent Dyes; D053803:Forensic Genetics; D010207:Papaver / Q000235:genetics*; D011110:Polymorphism, Genetic
D018744:DNA, Plant; D005456:Fluorescent Dyes