title: Stress response of Nidula niveo-tomentosa to UV-A light.
abstract: Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveotomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.
authors.affiliation: Zentrum Angewandte Chemie, Institut für Lebensmittelchemie der Leibniz Universitiit Hannover, Wunstorfer Strasse 14, D-30453 Hannover, Germany.
authors.forename: Daniela E
authors.initials: DE
authors.lastname: Taupp
authors.forename: Manfred
authors.initials: M
authors.lastname: Nimtz
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Berger
authors.forename: Holger
authors.initials: H
authors.lastname: Zorn
mesh_terms: D000363:Agaricales / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D018533:Biomass; D002074:Butanones / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D014466:Ultraviolet Rays
chemical_list: D002074:Butanones; D005656:Fungal Proteins; C035522:raspberry ketone