[Xiaojin Wan inhibits the expression of COX-2 in prostate tissues of prostatitis pain rats].
OBJECTIVE
To study the effect of Xiaojin Wan on the expression of COX-2 in the prostate tissues of rats with prostatitis pain, and the action mechanism of the drug alleviating the prostatitis pain.


METHODS
Sixty male Wistar rats were randomized into two groups, 10 as blank controls, injected with aqua pro injection into the ventral part of prostate, and the other 50 as prostatitis pain models, given complete Freund's adjuvant (CFA). Three days later, the pain model rats were again equally divided into 5 subgroups: model control, Celecoxib Capsules, high-, median- and low-dose Xiaojin Wan, receiving intragastric administration of distilled water, Celecoxib Capsules and different doses of Xiaojin Wan respectively for 4 weeks. Then they were killed, the harvested tissues fixed with 10% paraformaldehyde and the changes of the COX-2 expression in the prostate detected with the immunohistochemical technique and graphics video analysis system.


RESULTS
The expression of COX-2 was strong in the model group, significantly lower in the high- and median-dose and the Celecoxib Capsules groups than in the model control (P < 0.01) as well as in the high-dose than in the median- and low-dose groups (P < 0.01).


CONCLUSIONS
Xiaojin Wan may alleviate prostatitis pain by inhibiting the expression of COX-2 in prostate tissues.
Qu
Xiao-Wei
XW
School ofpostgratuate, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, China. doc521@sina.com
Zhang
Shu-Wu
SW
Zhang
Pei-Hai
PH
Yin
Jing
J
D000818:Animals; D002908:Chronic Disease; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D007150:Immunohistochemistry; D008297:Male; D010146:Pain / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology; D008517:Phytotherapy; D011467:Prostate / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D011472:Prostatitis / Q000150:complications / Q000188:drug therapy* / Q000201:enzymology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; C539009:xiaojin wan; D051546:Cyclooxygenase 2