Immunogenicity and protective efficacy of tuberculosis subunit vaccines expressing PPE44 (Rv2770c).
In this study we have evaluated the vaccine potential of a Mycobacterium tuberculosis antigen of the PPE protein family, namely PPE44 (Rv2770c). PPE44-specific immune responses could be detected in mice acutely, chronically and latently infected with M. tuberculosis. Vaccination of mice with a plasmid DNA vaccine coding for PPE44 or recombinant PPE44 protein formulated in adjuvant generated strong cellular and humoral immune responses; immunodominant T cell epitopes were identified. Most importantly, vaccination of mice with both subunit vaccines followed by an intratracheal challenge with M. tuberculosis resulted in a protective efficacy comparable to the one afforded by BCG. Taken together these results indicate that PPE44 of M. tuberculosis is a protective antigen that could be included in novel subunit TB vaccines and that warrants further analysis.
Romano
Marta
M
Mycobacterial Immunology, Pasteur Institute, Wetenschappelijk Instituut voor Volksgezondheid, Brussels, Belgium.
Rindi
Laura
L
Korf
Hannelie
H
Bonanni
Daniela
D
Adnet
Pierre-Yves
PY
Jurion
Fabienne
F
Garzelli
Carlo
C
Huygen
Kris
K
D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000917:Antibody Formation / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001500:BCG Vaccine / Q000276:immunology / Q000627:therapeutic use; D016207:Cytokines / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D016219:Immunotherapy, Adoptive; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D032581:Tuberculosis Vaccines / Q000235:genetics* / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D037521:Virulence Factors / Q000276:immunology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D016207:Cytokines; D000939:Epitopes; C524728:PPE44 protein, Mycobacterium tuberculosis; D012333:RNA, Messenger; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D037521:Virulence Factors; D004247:DNA