Innovative method for determining glycogen content in the Pacific oyster (Crassostrea gigas) by ion chromatography.
A precise analytical assay was developed and validated for the determination of Pacific oyster glycogen using ion chromatography. The Pacific oyster glycogen was quantified by the determination of glucose-6-phosphate (Glc-6-P), which was derived from glucose that was hydrolyzed from glycogen. Glc-6-P, adenosine triphosphate, and adenosine diphosphate were separated by ion chromatography. The method was validated over the curve range 0.5-100mg/L for the abovementioned analytes. The recoveries were between 95% and 102%. The relative standard deviations (RSDs, c=10mg/L, n=9) were less than 4.37%. Unlike a traditional method, this validated method was inexpensive and stable.
Gao
Rui-Chang
RC
College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China.
Yuan
Li
L
Wang
Qi
Q
Xue
Yong
Y
Feng
Hui
H
Yin
Li-Ang
LA
Wang
Jing-Feng
JF
Xue
Chang-Hu
CH
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods*; D049896:Crassostrea / Q000737:chemistry*; D019298:Glucose-6-Phosphate / Q000032:analysis*; D006003:Glycogen / Q000032:analysis*
D019298:Glucose-6-Phosphate; D006003:Glycogen