title: Manufacture of stable palladium and gold nanoparticles on native and genetically engineered flagella scaffolds.
abstract: The use of bacterial flagella as templates for the immobilization of Pd and Au nanoparticles is described. Complete coverage of D. desulfuricans flagellar filaments by Pd(0) nanoparticles was obtained via the H(2)-mediated reduction of Pd(NH3)4]Cl2 but similar results were not obtained using HAuCl4. The introduction of additional cysteine-derived thiol residues in the E. coli FliC protein increased Au(III) sorption and reduction onto the surface of the flagellar filament and resulted in the production of stabilized Au(0) nanoparticles of approximately 20-50 nm diameter. We demonstrate the application of molecular engineering techniques to manufacture biologically passivated Au(0) nanoparticles of a size suitable for catalytic applications.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B152TT, UK.
authors.forename: Kevin
authors.initials: K
authors.lastname: Deplanche
authors.forename: Richard D
authors.initials: RD
authors.lastname: Woods
authors.forename: Iryna P
authors.initials: IP
authors.lastname: Mikheenko
authors.forename: R Elizabeth
authors.initials: RE
authors.lastname: Sockett
authors.forename: Lynne E
authors.initials: LE
authors.lastname: Macaskie
mesh_terms: D002384:Catalysis; D003460:Crystallization / Q000379:methods; D003545:Cysteine / Q000737:chemistry / Q000235:genetics; D045285:Desulfovibrio desulfuricans / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005407:Flagella / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005408:Flagellin / Q000235:genetics / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006864:Hydrogenase / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010084:Oxidation-Reduction; D010165:Palladium / Q000737:chemistry*; D015202:Protein Engineering / Q000379:methods; D013499:Surface Properties
chemical_list: D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D005408:Flagellin; D010165:Palladium; D006046:Gold; D006864:Hydrogenase; D003545:Cysteine