title: The antiinflammatory, gastroprotective and antioxidant activities of Hieracium gymnocephalum extract.
abstract: The present study investigated the antiinflammatory, gastroprotective and antioxidant activities of a CH(2)Cl(2) extract of western Balkan endemic Hieracium gymnocephalum Griseb. ex Pant. (Compositae). The carrageenan-induced rat paw oedema test was used as an experimental model for screening the antiinflammatory activity. The extract was administrated p.o. in doses of 25, 50, 100 and 200 mg/kg to rats and its effects compared with indomethacin, used p.o. as a reference drug. The results showed that the investigated extract reduced the oedema in a concentration-dependent manner. The obtained antiinflammatory effect was 5.9%, 11.7%, 31.2% and 44.1% at doses of 25, 50, 100 and 200 mg/kg, respectively, being statistically significant at a dose of 100 mg/kg. Indomethacin had a strong antiinflammatory effect of 73.4% at a dose of 8 mg/kg, but caused large gastric lesions. When the plant extract in the highest tested dose (200 mg/kg) was concomitantly given with indomethacin, the antiinflammatory effect was slightly enhanced, but the gastric lesions were significantly reduced. The antioxidant activity of the H. gymnocephalum extract, investigated using DPPH radical assay, OH-radical assay and TBA-test, was not substantial.
authors.affiliation: Institute of Pharmacognosy, Faculty of Pharmacy, Vojvode Stepe 450, 11221 Belgrade, Serbia. silvana@pharmacy.bg.ac.yu
authors.forename: Silvana
authors.initials: S
authors.lastname: Petrović
authors.forename: Silva
authors.initials: S
authors.lastname: Dobrić
authors.forename: Neda
authors.initials: N
authors.lastname: Mimica-Dukić
authors.forename: Natasa
authors.initials: N
authors.lastname: Simin
authors.forename: Jelena
authors.initials: J
authors.lastname: Kukić
authors.forename: Marjan
authors.initials: M
authors.lastname: Niketić
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy*; D007213:Indomethacin / Q000633:toxicity; D007249:Inflammation / Q000188:drug therapy; D015227:Lipid Peroxidation; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy
chemical_list: D000893:Anti-Inflammatory Agents; D000897:Anti-Ulcer Agents; D000975:Antioxidants; D010936:Plant Extracts; D007213:Indomethacin