[Protective activity of secreted proteins of Streptococcus pneumoniae and Klebsiella pneumoniae].
Protective efficacy of secreted proteins of Streptococcus pneumoniae and Klebsiella pneumoniae cultivated on cardiocerebral broth and semisynthetic growth medium respectively was studied in vivo. Fraction with molecular weight 30 - 50 kDa obtained by the method of membrane fractionation had high protective efficacy. Two-dose immunization of mice with this fraction provided 80 - 100% protection from infection by homologous strains of S. pneumoniae and K. pneumoniae. Cross-protective activity of the fraction was revealed when infecting immunized mice by different K-types of K. pneumoniae. Blood sera of mice immunized with 30 - 50 kDa fraction possessed preventive features protecting from infection 90% of animals while 100% of death in the control group. It was determined that protective efficacy of the mentioned fraction was determined by protein-containing antigens because proteolytic disruption of the protein component resulted in loss of protective properties of the preparation.
Trishin
A V
AV
Donenko
F V
FV
Kurbatova
E A
EA
Voiushin
K E
KE
Kiselevskiĭ
M V
MV
Egorova
N B
NB
Gruber
I M
IM
Semenova
I B
IB
Semenov
B F
BF
D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D003470:Culture Media; D007106:Immune Sera / Q000008:administration & dosage; D007116:Immunization, Passive; D007710:Klebsiella Infections / Q000517:prevention & control*; D007711:Klebsiella pneumoniae / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D011008:Pneumococcal Infections / Q000517:prevention & control*; D013296:Streptococcus pneumoniae / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D007106:Immune Sera