[Isolation, purification and biological activities of Arnebia euchroma glycosaminoglycans].
OBJECTIVE
To study the methods of extraction, isolation, purification and biological activities of Arnebia euchroma glycosaminoglycans (AEG).


METHODS
AEG was purified by distilled water extraction, ethanol fractionation, Sephadex column chromatography. The purity and molecular weight and concentration of AEG were measured by HPLC; We divided the experiment into Physiological Saline group and the other eight groups with different doses of AEG, established RT-PRC method to observe the anti-HPV effect after their actions on the verruca tissues.


RESULTS
Using HPLC, the group of AEG was divided into two glycosaminoglycans with different molecular weight as 27336 and 1152. Bioassay results showed that AEG had anti-HPV-DNA activity, the lowest effective concentration was 0.781 mg/ml.


CONCLUSIONS
AEG extracted by this method is a mixture with two molecular weight glycosaminoglycans, and has obvious anti-HPV effect.
Deng
Yuan-Hui
YH
The Second Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510120, China. yhdeng62@126.com
Wang
Hai-Lan
HL
Han
Ling
L
D028082:Boraginaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004279:DNA, Viral / Q000187:drug effects / Q000302:isolation & purification; D006025:Glycosaminoglycans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D052163:Human papillomavirus 6 / Q000187:drug effects* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010946:Plants, Medicinal / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods
D004279:DNA, Viral; D006025:Glycosaminoglycans