Structure of the glycerol phosphate-containing O-specific polysaccharide and serological studies on the lipopolysaccharides of Citrobacter werkmanii PCM 1548 and PCM 1549 (serogroup O14).
The O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of Citrobacter werkmanii PCM 1548 and PCM 1549 (serogroup O14) and found to contain D-glucose, D-glucosamine and glycerol-1-phosphate in molar ratios 2 : 2 : 1. Based on methylation analysis and 1H and 13C nuclear magnetic resonance spectroscopy data, it was established that the O-specific polysaccharides from both strains have the identical branched tetrasaccharide repeating unit with 3,6-disubstituted GlcNAc, followed by 2,4-disubstituted Glc residues carrying at the branching points lateral residues of Glc and GlcNAc at positions 6 and 2, respectively. Glycerol-1-phosphate is linked to position 6 of the chain Glc. All sugars have a beta configuration, except for the side-chain Glc, which is alpha. Serological studies revealed a close relatedness of the lipopolysaccharides of C. werkmanii PCM 1548 and PCM 1549, both belonging to serogroup O14. In immunoblotting, anti-C. werkmanii PCM 1548 serum showed no cross-reactivity with the O-polysaccharide bands of the lipopolysaccharides of Citrobacter youngae PCM 1550 (serogroup O16) and Hafnia alvei PCM 1207, also containing a lateral glycerol phosphate residue.
Katzenellenbogen
Ewa
E
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland. katsenel@iitd.pan.wroc.pl
Kocharova
Nina A
NA
Korzeniowska-Kowal
Agnieszka
A
Bogulska
Maria
M
Rybka
Jacek
J
Gamian
Andrzej
A
Kachala
Vadim V
VV
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002954:Citrobacter / Q000145:classification / Q000276:immunology*; D005994:Glycerophosphates / Q000737:chemistry; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000145:classification / Q000276:immunology*; D008745:Methylation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D012703:Serotyping
D002241:Carbohydrates; D005994:Glycerophosphates; D008070:Lipopolysaccharides; D019081:O Antigens