title: Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction.
abstract: OBJECTIVE
  Magnetic activated cell sorting (MACS) eliminates apoptotic spermatozoa based on the presence of externalized phosphatidylserine residues. We evaluated the outcome of male fertility treatment when intracytoplasmic sperm injection (ICSI) into human oocytes was performed with non-apoptotic MACS-selected spermatozoa.
  
  
  METHODS
  196 couples were treated by ICSI following spermatozoa preparation by MACS (study group; 122 couples) or density gradient centrifugation (DGC) (control group; 74 couples). Fertilization, cleavage, pregnancy, and implantation rates were analyzed.
  
  
  RESULTS
  The percentage of sperm with normal morphology after MACS selection was improved. Cleavage and pregnancy rates were higher, respectively, in the study group than in control. A slightly higher implantation rate was also observed in the study group.
  
  
  CONCLUSIONS
  MACS selection of human spermatozoa increased cleavage and pregnancy rates in oligoasthenozoospermic ART cases. This novel method for selecting non-apoptotic spermatozoa for ICSI is safe and reliable, and may improve the assisted reproduction outcome.
authors.affiliation: Embryology, MAYA Center for Assisted Reproductive Technologies, Ankara, Turkey. kerem@dirican.tr.tc
authors.forename: Enver Kerem
authors.initials: EK
authors.lastname: Dirican
authors.forename: Osman Denizhan
authors.initials: OD
authors.lastname: Ozgün
authors.forename: Süleyman
authors.initials: S
authors.lastname: Akarsu
authors.forename: Kadir Okhan
authors.initials: KO
authors.lastname: Akin
authors.forename: Ozge
authors.initials: O
authors.lastname: Ercan
authors.forename: Mukaddes
authors.initials: M
authors.lastname: Uğurlu
authors.forename: Cağri
authors.initials: C
authors.lastname: Camsari
authors.forename: Oya
authors.initials: O
authors.lastname: Kanyilmaz
authors.forename: Adnan
authors.initials: A
authors.lastname: Kaya
authors.forename: Ali
authors.initials: A
authors.lastname: Unsal
mesh_terms: D017209:Apoptosis; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D005260:Female; D005298:Fertility; D005306:Fertilization; D006801:Humans; D008280:Magnetics; D008297:Male; D010718:Phosphatidylserines / Q000737:chemistry; D011247:Pregnancy; D018873:Pregnancy Rate; D027724:Reproductive Techniques, Assisted; D012661:Semen / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology*; D016896:Treatment Outcome
chemical_list: D010718:Phosphatidylserines