CHD1 assumes a central role during follicle development.
During Bombyx mori follicle development, fine-tuning of chorion gene expression is under the control of bidirectional promoters. In this work, we show that the silkmoth chromo-helicase/ATPase-DNA binding protein 1 (CHD1) ortholog is responsible for repositioning of nucleosomes on chorion promoters, where the factor binds specifically. Chorion genes, occupying a single chromosomal locus, rely on an almost identical set of cis elements for their differential expression. As a direct consequence of remodeling, interaction of C/EBP and TFIID with promoter elements is facilitated and ultimately leads to initiation of transcription. Appending of methylation marks to H3K4 in a temporal-specific manner is dependent on CHD1 binding to cognate cis elements and signifies gene activation. Overall, CHD1 is a critical factor for proper development of the follicular epithelium in terms of whole-cell chromatin arrangement.
Papantonis
Argyris
A
Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, Greece.
Tsatsarounos
Sotiris
S
Vanden Broeck
Jozef
J
Lecanidou
Rena
R
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000196:embryology* / Q000235:genetics; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D002823:Chorion / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D018983:DNA Footprinting; D016373:DNA, Antisense / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D024003:HMGA Proteins / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008745:Methylation; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism; D006080:Ovarian Follicle / Q000196:embryology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D035362:Transcription Factor TFIID / Q000378:metabolism; D015533:Transcriptional Activation
D022762:CCAAT-Enhancer-Binding Proteins; D016373:DNA, Antisense; D024003:HMGA Proteins; D006657:Histones; D019476:Insect Proteins; D009707:Nucleosomes; D035362:Transcription Factor TFIID