Upregulation of CD94 on CD8+T cells in anterior chamber-associated immune deviation.
BACKGROUND
CD8+ regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The phenotype and characteristics of CD8+Treg in ACAID remain only poorly understood. Recent studies have reported that the CD94-Qa-1 system is implicated in the induction of ACAID CD8+Treg, but the functions and characteristics of CD8+CD94+T cells remain unclear.


RESULTS
Both mRNA and protein of CD94 and NKG2A were markedly up-regulated on splenic CD8+T cells of ACAID mice compared with controls. Flow cytometric analysis showed that very few CD8+CD94+T cells express granzyme B, perforin and Foxp3. CD8+CD94+T cells, but not CD8+CD94-T cells, magnetically isolated from the spleens of ACAID mice, produced large amounts of TGF-beta1 and exhibited suppressive activity in vitro. Neutralization of TGF-beta1 caused reversal of suppression mediated by CD8+CD94+T cells.


CONCLUSIONS
CD8+CD94+T cells from ACAID mice exhibited suppressive activity in association with enhanced expression of TGF-beta1, suggesting that CD8+Treg are mainly distributed in CD94+T cell subpopulations.
He
Hao
H
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangzhou, PR China. hehao3000@qq.com
Yang
Peizeng
P
Jiang
Liqiong
L
Zhang
Junfeng
J
Zhao
Changlin
C
Chen
Lina
L
Lin
Xiaomin
X
Zhou
Hongyan
H
Kijlstra
Aize
A
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D053804:Granzymes / Q000378:metabolism; D007108:Immune Tolerance; D007165:Immunosuppression Therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D051920:NK Cell Lectin-Like Receptor Subfamily D / Q000276:immunology*; D054353:Perforin / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation
D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D051920:NK Cell Lectin-Like Receptor Subfamily D; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D016212:Transforming Growth Factor beta; D054353:Perforin; D053804:Granzymes