[Effect of enalapil on renal interstitial fibrosis in rats with unilateral ureteral obstruction].
OBJECTIVE
To investigate the effect of enalapril on renal interstitial fibrosis in rats with unilateral ureteral obstruction(UUO).


METHODS
UUO model was induced by ligating the left ureter in rats. Male Sprague-Dawley(SD) rats were randomly divided into a sham-operated group(n=16), a UUO model group(n=24), and an enalapril treated group(n=24). The rats were treated with 10 mg/kg.d by gastric gavage in the enalapril treated group from 24 h before the operation, and the rats were treated with the identical dose of normal saline in the other 2 groups. The rats were sacrificed at 3,7,14, and 21 days after UUO. Pathological changes of the renal tissue were observed by HE and Masson staining, the mRNA expression of collagen I (Col I) was detected by real-time PCR, and the protein expression of connective tissue growth factor (CTGF) was detected by Western blot.


RESULTS
The renal interstitial damage index, relative collagen area and the expression of Col I mRNA and CTGF in the renal tissues in the model group increased with the prolongation of obstruction. Enalapril significantly reduced the renal interstitial damage index and relative collagen area, and inhibted the expression of Col I mRNA and CTGF. There was significant difference on day 3,7,and 14 (P<0.05), but not on day 21 (P>0.05).


CONCLUSIONS
Enalapril significantly attenuates renal interstitial fibrosis by supressing the expression of Col I mRNA and CTGF.
Wang
Lin-na
LN
Department of Nephrology, Xiangya Hospital,Central South University, Changsha 410008, China.
Tao
Li-jian
LJ
Ning
Wang-bin
WB
D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D055513:Connective Tissue Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D004656:Enalapril / Q000627:therapeutic use*; D008297:Male; D009395:Nephritis, Interstitial / Q000209:etiology / Q000517:prevention & control*; D009400:Nephrosclerosis / Q000209:etiology / Q000517:prevention & control*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014517:Ureteral Obstruction / Q000150:complications
C526316:CCN2 protein, rat; D024042:Collagen Type I; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor; D004656:Enalapril