[Monosaccharide composition analysis and its content determination of polysaccharides from Rhaponticum uniforum].
OBJECTIVE
To analyze the monosaccharide composition in the polysaccharides from Rhaponticum uniforum, determine the content of monosaccharide, and provide some references for further research.


METHODS
The monosaccharide composition was determined by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Phenol-sulfuric acid method was used for the determination of the content of polysaccharide.


RESULTS
The monosaccharides composition in polysaccharides from R. uniforum are glucose, arabonose and fructose. Their molar ratios are 1 : 1.61 : 2.21. The content of polysaccharide is 95.78%, taking the mixture of monosaccharide compositions as reference substances.


CONCLUSIONS
HPAEC-PAD can be used to analyze the monosaccharide composition in the polysaccharide with high precision, and the method of phenol-sulfuric acid is simple, convenient and reliable.
Li
Fa-Sheng
FS
College of Clinical Laboratory, Dalian Medical University, Dalian 116044, China. lfs920@sohu.com
Xu
Heng-Gui
HG
Yan
Xiao-Mei
XM
Li
Ming-Yang
MY
Liu
Hui
H
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D043843:Leuzea / Q000737:chemistry*; D009005:Monosaccharides / Q000032:analysis* / Q000302:isolation & purification; D011134:Polysaccharides / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004365:Drugs, Chinese Herbal; D009005:Monosaccharides; D011134:Polysaccharides