Simple enrichment and independent cultures to expand bacterial community analysis from Gedongsongo hot spring.
Denaturing gradient gel electrophoresis was used to identify bacterial communities from Gedongsongo hot spring, Indonesia, by culture-independent and -dependent procedures. Predominant species were found to be closely related to beta-proteobacteria, particularly from genus Ralstonia and Delftia. Other species present include alpha- and gamma-proteobacteria and Thermus groups. Some of the strains showed unique 16S rDNA sequences.
Aminin
Agustina Lulustyaningati Nurul
AL
Biochemistry Research Division, Faculty of Mathematics and Natural Science, Institut Teknologi Bandung, Bandung, Indonesia.
Warganegara
Fida Madayanti
FM
Aditiawati
Pingkan
P
Akhmaloka
D001419:Bacteria / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D045482:Hot Springs; D007214:Indonesia; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014871:Water Microbiology
D004269:DNA, Bacterial