[Biotransformation of furannoligularenone by hairy root cultures of Polygonum multiflorum].
OBJECTIVE
To investigate the biotransformation of furannoligularenone by hairy roots of Polygonum multiflorum.


METHODS
Furannoligularenone was added to the medium of hairy roots of P. multiflorum after precultured for 9 days, then they were co-cultured. The products were isolated and identified on the basis of their physical chemical properties and spectroscopic data. The T-C curve of biotransformation was investigated with HPLC.


RESULTS
The hairy roots of P. multiflorum transformed the substrate to two products, 3-oxo-eremophila-1,7(11)-dien-12,8-olide(II) and 3-oxo-8-hydroxy-eremophila-1,7(11)-dien-12,8-olide(III). After co-cultured for 3 days, the mole conversion ratio of the substrate reached the highest (27.2%).


CONCLUSIONS
It's possible to biotransform furannoligularenone by hairy roots of P. multiflorum.
Yan
Chun-Yan
CY
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. ycybridge@163.com
Ma
Wei-Li
WL
Yan
Wen-Wen
WW
Yu
Rong-Min
RM
D001711:Biotransformation; D003470:Culture Media; D005663:Furans / Q000302:isolation & purification / Q000378:metabolism*; D015394:Molecular Structure; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism; D028502:Polygonum / Q000254:growth & development / Q000378:metabolism*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D046509:Tissue Culture Techniques / Q000379:methods
D003470:Culture Media; D005663:Furans; D012717:Sesquiterpenes; C544118:furannoligularenone