Isolation and identification of a new hypocrellin A-producing strain Shiraia sp. SUPER-H168.
A new hypocrellin A-producing strain, Shiraia sp. SUPER-H168, was isolated from tissues of bamboo, Brachystachyum densiflorum. The morphology of this strain was characterized with a light microscope and a scanning electronic microscope. The mycelia, conidia, pycnidia of fungus were observed. Small pycnidia (10-20 microm in length) full of small conidia appeared on the mycelia, which were first reported in this study. The 18S rDNA region of this strain was amplified and sequenced. Then a neighbor-joining tree of 18S rDNA was constructed. According to the result of analysis, the strain SUPER-H168 was proved to belong to the genus Shiraia. Hypocrellin A was produced by solid-state fermentation, extracted by acetone, isolated by preparative RP-HPLC, and identified by RP-HPLC, ESI-MS and ultraviolet-visible absorbing scanning with diode array detection. The HA production could reach 2.02 mg/g dry solid substrate.
Liang
Xiao-Hui
XH
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Road 1800, Wuxi 214122, Jiangsu, China.
Cai
Yu-Jie
YJ
Liao
Xiang-Ru
XR
Wu
Kang
K
Wang
Lei
L
Zhang
Da-Bing
DB
Meng
Qiang
Q
D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D031723:Bambusa / Q000382:microbiology*; D002681:China; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005285:Fermentation; D008969:Molecular Sequence Data; D010569:Perylene / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D019800:Phenol; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D011809:Quinones; D012337:RNA, Ribosomal, 18S; D019800:Phenol; D010569:Perylene; C048388:hypocrellin A