Reduced acute inflammatory responses to microgel conformal coatings.
Implantation of synthetic materials into the body elicits inflammatory host responses that limit medical device integration and biological performance. This inflammatory cascade involves protein adsorption, leukocyte recruitment and activation, cytokine release, and fibrous encapsulation of the implant. We present a coating strategy based on thin films of poly(N-isopropylacrylamide) hydrogel microparticles (i.e. microgels) cross-linked with poly(ethylene glycol) diacrylate. These particles were grafted onto a clinically relevant polymeric material to generate conformal coatings that significantly reduced in vitro fibrinogen adsorption and primary human monocyte/macrophage adhesion and spreading. These microgel coatings also reduced leukocyte adhesion and expression of pro-inflammatory cytokines (TNF-alpha, IL-1beta, MCP-1) in response to materials implanted acutely in the murine intraperitoneal space. These microgel coatings can be applied to biomedical implants as a protective coating to attenuate biofouling, leukocyte adhesion and activation, and adverse host responses for biomedical and biotechnological applications.
Bridges
Amanda W
AW
Petit Institute for Bioengineering and Bioscience, 315 Ferst Drive, 2314 IBB, Atlanta, GA 30332-0363, USA.
Singh
Neetu
N
Burns
Kellie L
KL
Babensee
Julia E
JE
Andrew Lyon
L
L
García
Andrés J
AJ
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000009:adverse effects; D006801:Humans; D020100:Hydrogels / Q000009:adverse effects; D018836:Inflammation Mediators / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D018836:Inflammation Mediators; D014409:Tumor Necrosis Factor-alpha