Electrophysiological properties of embryonic stem cells during differentiation into cardiomyocyte-like cell types.
The method described here to differentiate mouse embryonic stem (ES) cells into cardiomyocytes is adapted from Maltsev et al. and results in a high percentage of spontaneously beating cardiomyocyte-like cells. In order to determine to what extent the differentiating ES cells resemble true cardiomyocytes, the cells were electrophysiologically characterized during differentiation, using the whole-cell variant of the patch-clamp technique. Action potentials (APs) and membrane currents were recorded and analyzed off-line to determine electrophysiological changes during development.
van Ginneken
Antoni C G
AC
Department of Experimental Cardiology and Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, The Netherlands.
Fijnvandraat
Arnoud C
AC
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D055724:Electrophysiological Phenomena; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology*