The rapid identification of isoflavonoids from Belamcanda chinensis by LC-NMR and LC-MS.
The use of hyphenated LC-NMR and LC-MS techniques for the purpose of directly identifying the major constituents of Belamcanda chinensis was investigated. Reversed-phase isocratic chromatography was performed using an acetonitrile-water solvent system on a C18 column. The NMR spectrum yielded five main peaks, whose analysis revealed them to be 5, 6, 7, 3'-tetrahydroxy-4'-methoxyisoflavone (1), tectorigenin (2), iristectorigenin A (3), irigenin (4), and irisflorentine (5). The identification of these constituents was confirmed by performing LC-ESI-MS experiment. This study shows that hyphenated LC-NMR and LC-MS can be used for the rapid (70 min) identification of the isoflavonoids.
Kang
Suk Woo
SW
Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Korea.
Kim
Min Cheol
MC
Kim
Chul Young
CY
Jung
Sang Hoon
SH
Um
Byung Hun
BH
D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D027621:Iridaceae / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D018517:Plant Roots / Q000737:chemistry; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization
D007529:Isoflavones; D012997:Solvents; D000431:Ethanol