title: Relationship between nm23H1 genetic instability and clinical pathological characteristics in Chinese digestive system cancer patients.
abstract: OBJECTIVE
  To study the relationship between nm23H1 gene genetic instability and its clinical pathological characteristics in Chinese digestive system cancer patients.
  
  
  METHODS
  Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to analyze the microsatellite instability (MSI) and loss of heterozygosity (LOH). Immunohistochemistry was employed to detect the expression of nm23H1.
  
  
  RESULTS
  The MSI was higher in TNM stage I + II than in stage III + IV of gastric, colonic and gallbladder carcinomas. The LOH was higher in TNM stage III + IV than in stage I + II of gastric, colonic and hepatocellular carcinomas. Lymphatic metastasis was also observed. The expression of nm23H1 protein was lower in TNM stage III + IV than in stage I + II of these tumors and in patients with lymphatic metastasis.The nm23H1 protein expression was higher in the LOH negative group than in the LOH positive group.
  
  
  CONCLUSIONS
  MSI and LOH may independently control the biological behaviors of digestive system cancers. MSI could serve as an early biological marker of digestive system cancers. Enhanced expression of nm23H1 protein could efficiently inhibit cancer metastasis and improve its prognosis. LOH mostly appears in late digestive system cancer.
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Yang
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.forename: Jin-Xing
authors.initials: JX
authors.lastname: Chen
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Sun
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Li
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Lu
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Su
authors.forename: Xin-Qiu
authors.initials: XQ
authors.lastname: Lin
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Li
mesh_terms: D044466:Asian People / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000208:ethnology / Q000235:genetics; D002681:China; D003110:Colonic Neoplasms / Q000208:ethnology / Q000235:genetics; D004067:Digestive System Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D005706:Gallbladder Neoplasms / Q000208:ethnology / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D006801:Humans; D008113:Liver Neoplasms / Q000208:ethnology / Q000235:genetics; D019656:Loss of Heterozygosity; D008207:Lymphatic Metastasis; D053842:Microsatellite Instability; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*; D009367:Neoplasm Staging; D010641:Phenotype; D013274:Stomach Neoplasms / Q000208:ethnology / Q000235:genetics
chemical_list: D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human