title: [Impact of different emulated acupuncture-needle manipulations on blood pressure and myocardial angiotensin II content in spontaneous hypertension rats].
abstract: OBJECTIVE
  To observe the effect of different emulated acupuncture-needle manipulations on blood pressure and myocardial angiotensin (Ang) II content in spontaneously hypertensive rats (SHR), so as to seek a better acupuncture parameter for clinical treatment of hypertension.
  
  
  METHODS
  Thirty-three SHR were randomly divided into mild-stimulation group (n=8), moderate-stimulation group (n=8), heavy-stimulation group (n=8) and model group (n=9). Additionally, other 8 normal rats were made up of control group. Blood pressure (BP) was determined by using a non-invasive BP detection system. Myocardial Ang II content was assayed by radioimmunoassay. The emulated acupuncture-needle twirling angles and frequencies of 144 degrees, 75 r/min; 255 degrees, 111 r/min; and 360 degrees, 140 r/min in mild, moderate and heavy manipulations were applied to bilateral "Zusanli" (ST 36) and "Taichong" (LR 3) for 3 min, once daily for 3 weeks, with an interval of 2 days between every two weeks.
  
  
  RESULTS
  Compared with pre-therapy, BP values of both heavy-stimulation and model groups increased significantly (P<0.01); and compared with model group, BP values of mild-stimulation and moderate-stimulation groups decreased significantly after the therapy (P<0.05), but were still higher than that of control group (P<0.05). In addition, myocardial Ang II contents of mild-stimulation, moderate-stimulation, heavy-stimulation and model groups were significantly higher than that of control group (P<0.05). No significant differences were found between mild-stimulation and moderate-stimulation groups, and between heavy-stimulation and model groups after therapy in BP, and among mild-stimulation, moderate-stimulation, heavy-stimulation and model groups in myocardial Ang II contents (P>0.05).
  
  
  CONCLUSIONS
  Mild and moderate manipulations of ST36 and LR3 have apparent influence on BP, but no apparent influence on myocardial Ang II content.
authors.affiliation: College of Acu-moxibustion and Massage, Shanghai University of Chinese Medicine, Shanghai 201203, China. yhyabcd@sina.com
authors.forename: Hua-Yuan
authors.initials: HY
authors.lastname: Yang
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Zhong
authors.forename: Tang-Yi
authors.initials: TY
authors.lastname: Liu
authors.forename: Le
authors.initials: L
authors.lastname: Kuai
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
mesh_terms: D015669:Acupuncture Points; D015670:Acupuncture Therapy / Q000379:methods*; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001794:Blood Pressure; D006973:Hypertension / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D000804:Angiotensin II