title: Retrospective study of adenovirus in autopsied pulmonary tissue of pediatric fatal pneumonia in South China.
abstract: BACKGROUND
  Adenovirus are the important pathogen of pediatric severe pneumonia. The aim of this study is to analyze the infection, subtype and distribution of adenovirus in autopsied pulmonary tissue of fatal pneumonia in infants and children, and the relationships between adenovirus infection and respiratory illness in South China.
  
  
  METHODS
  Nested PCR was performed on DNA extracted from autopsied lung tissue from patients who died of severe pneumonia, and the positive nested PCR products were cloned and sequenced. The adenovirus in autopsied pulmonary tissue was also analyzed by immunohistochemistry assay in a blind way.
  
  
  RESULTS
  In the 175 autopsied pulmonary tissues, the positive percentage of adenovirus was 9.14% (16/175) and 2.29% (4/175) detected with nested PCR and immunohistochemistry, respectively. There are three cases of adenovirus serotype 3, twelve cases of adenovirus serotype 4 and one case of serotype 41 determined by sequencing of the cloned positive nested PCR products.
  
  
  CONCLUSIONS
  Adenovirus is an important cause of severe pneumonia, and these data suggest that adenovirus serotype 4 might be an important pathogen responsible for the fatal pneumonia in Guangzhou, South China.
authors.affiliation: Guangzhou Children's Hospital, Guangzhou Medical College, Guangzhou, 510120, Guangdong, PR China. ouzhiying@yahoo.com.cn
authors.forename: Zhi-Ying
authors.initials: ZY
authors.lastname: Ou
authors.forename: Qi-Yi
authors.initials: QY
authors.lastname: Zeng
authors.forename: Feng-Hua
authors.initials: FH
authors.lastname: Wang
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Xia
authors.forename: Jun-Peng
authors.initials: JP
authors.lastname: Lu
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Xia
authors.forename: Si-Tang
authors.initials: ST
authors.lastname: Gong
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.forename: Jian-Tao
authors.initials: JT
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000258:Adenovirus Infections, Human / Q000453:epidemiology* / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics / Q000472:pathogenicity*; D001344:Autopsy; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008168:Lung / Q000473:pathology / Q000821:virology*; D008297:Male; D011024:Pneumonia, Viral / Q000453:epidemiology* / Q000209:etiology* / Q000821:virology; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
chemical_list: D004279:DNA, Viral