Structural analysis of point mutations in the Hairless gene and their association with the activity of the Hairless protein.
The Notch signaling pathway (NSP) is an important intercellular communication mechanism that regulates embryo development and adult physiological functions. The Hairless (H) protein is a powerful antagonist of the NSP by its interaction with the Suppressor of Hairless (Su[H]) protein, recruiting the corepressors Gro and CtBP. In the present work, we examined the role of several important amino acids in different H protein domains analyzing four mutant lines of Drosophila melanogaster. The mutant alleles were evaluated by single-strand conformational polymorphism (SSCP) analysis and we located mutated regions at different positions along the sequence of the Hairless gene.
Paz-Gómez
Daniel
D
Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Km. 9.5, Carretera Morelia-Zinapécuaro, C.P. 58893, La Palma, Tarímbaro, Michoacán, Mexico.
Baizabal-Aguirre
Víctor Manuel
VM
Valdez-Alarcón
Juan José
JJ
Cajero-Juárez
Marcos
M
Nagel
Anja C
AC
Preiss
Anette
A
Maier
Dieter
D
Bravo-Patiño
Alejandro
A
D000595:Amino Acid Sequence; D000818:Animals; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000235:genetics* / Q000502:physiology; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D051880:Receptors, Notch / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
D029721:Drosophila Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; D014157:Transcription Factors; C076560:H protein, Drosophila