Role of immunoexpression of nitric oxide synthases by Hodgkin and Reed-Sternberg cells on apoptosis deregulation and on clinical outcome of classical Hodgkin lymphoma.
Hodgkin and Reed-Sternberg (H-RS) cells of classical Hodgkin lymphoma (cHL) present an impaired expression of immunoglobulin genes, but escape apoptotic death. We investigated whether nitric oxide synthases (NOS) are expressed by H-RS cells, studied their association with EBV status and the expression of apoptotic proteins, and investigated their relationship to the clinical outcome of 171 patients. NOS1 and NOS2 were expressed in a large number of cases, whereas NOS3 expression was not detected. Positive associations were found between NOS1 and p53, bax and NOS2, bcl-2 and NOS2, bax and p53, and between bax and fasL. Inverse correlations were established between EBV and NOS2 and between EBV and bcl-2. A shorter overall survival (OS) was associated with strong expression of NOS2. In conclusion, NOS are expressed by H-RS cells of cHL.
Campos
Antônio H J F M
AH
Department of Pathology, A. C. Camargo Cancer Treatment and Research Center, Sao Paulo, Brazil. ahcampos@hcancer.org.br
Aldred
Vera L
VL
Ribeiro
Karina C B
KC
Vassallo
José
J
Soares
Fernando A
FA
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006689:Hodgkin Disease / Q000276:immunology* / Q000503:physiopathology; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D016539:Reed-Sternberg Cells / Q000166:cytology / Q000201:enzymology* / Q000276:immunology*; D012189:Retrospective Studies; D016019:Survival Analysis; D055815:Young Adult
D007527:Isoenzymes; D019001:Nitric Oxide Synthase