title: Determination of flurbiprofen enantiomers in plasma using a single-isomer amino cyclodextrin derivative in nonaqueous capillary electrophoresis.
abstract: A nonaqueous capillary electrophoresis (NACE) assay was developed for the separation and determination of flurbiprofen enantiomers in plasma samples using 6-monodeoxy-6-mono(3-hydroxy)propylamino-beta-cyclodextrin as chiral selector. The nonaqueous background electrolyte was made up of 40 mM ammonium acetate in methanol (MeOH), and flufenamic acid was employed as internal standard. Solid-phase extraction was used for sample cleanup prior to the NACE separation. The NACE method reproducibility was optimized by evaluating different capillary washing sequences between runs. After having tested various conditions, trifluoroacetic acid (1 M) in MeOH was finally selected. Concerning the solid-phase extraction procedure, good and reproducible analyte recoveries were obtained using MeOH for protein denaturation and a polymeric phase combining hydrophobic interactions with anion exchange properties (Oasis) MAX) was selected as extraction sorbent. The method selectivity was not only demonstrated toward a blank plasma sample but also toward other non-steroidal anti-inflammatory drugs. The method was then successfully validated with respect to response function, trueness, precision, accuracy, linearity and limit of quantification.
authors.affiliation: Department of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, CHU, B36, Liège, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Rousseau
authors.forename: Matteo
authors.initials: M
authors.lastname: Pedrini
authors.forename: Patrice
authors.initials: P
authors.lastname: Chiap
authors.forename: Robert
authors.initials: R
authors.lastname: Ivanyi
authors.forename: Jacques
authors.initials: J
authors.lastname: Crommen
authors.forename: Marianne
authors.initials: M
authors.lastname: Fillet
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Servais
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000737:chemistry; D011232:Chemical Precipitation; D019075:Electrophoresis, Capillary / Q000379:methods*; D005480:Flurbiprofen / Q000097:blood* / Q000737:chemistry; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005480:Flurbiprofen