Methods for the purification of HQ-tagged proteins.
The HQ (H = histidine, Q = glutamine) tag is a small fusion tag that can be isolated using immobilized metal affinity columns. HQ-tagged proteins can be expressed and purified from bacterial cells under native and denaturing conditions, mammalian cells, insect cells, wheat germ and rabbit reticulocyte. Furthermore, HQ-tagged proteins can be purified using magnetic or non-magnetic resins in multiple formats from small to large-scale and manual or automated. In this chapter, we have described various protocols for the purification of HQ-tagged proteins.
Godat
Becky
B
Proteomics R&D, Promega Corporation, Fitchburg, WI, USA.
Engel
Laurie
L
Betz
Natalie A
NA
Johnson
Tonny M
TM
D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D002846:Chromatography, Affinity / Q000379:methods*; D005973:Glutamine / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D013058:Mass Spectrometry; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification*
D011993:Recombinant Fusion Proteins; D005973:Glutamine; D006639:Histidine