title: Identification of novel p53 target genes by cDNA AFLP in glioblastoma cells.
abstract: The p53 plays critical role in cellular functions such as cell cycle arrest and apoptosis. We overexpressed wild-type p53 (wt-p53) in U87 glioblastoma cells via recombinant adenovirus Ad-GFP-P53 which encodes p53 and green fluorescent protein. The transcript profiles were investigated using cDNA amplified fragment length polymorphism approach. Semi-quantitative RT-PCR and DNA sequencing results for the selected genes showed that Cathepsin B and cell cycle associated protein-1 or Caprin-I, genes were suppressed whereas Annexin-II gene overexpressed in response to the overexpression of wt-p53 gene. Our results suggest that these genes could be important mediators of p53-dependent tumor growth suppression in glioblastoma.
authors.affiliation: Department of Medical Genetics, Tehran University of Medical Sciences, Pour Sina Avenue, Tehran, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Saffari
authors.forename: Orkideh Saydi
authors.initials: OS
authors.lastname: Dinehkabodi
authors.forename: Seyed Hamid
authors.initials: SH
authors.lastname: Ghaffari
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Modarressi
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mansouri
authors.forename: Mansour
authors.initials: M
authors.lastname: Heidari
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D017306:Annexin A2 / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D002401:Cathepsin B / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D016158:Genes, p53; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017306:Annexin A2; C486562:CAPRIN1 protein, human; D018797:Cell Cycle Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D049452:Green Fluorescent Proteins; D002401:Cathepsin B