title: MRI retinovascular studies in humans: research in patients with diabetes.
abstract: OBJECTIVE
  To review existing applications of MRI for detecting blood-retinal barrier (BRB) damage and retinal oxygenation response abnormalities in patients with diabetes and highlight new information available from such applications.
  
  
  METHODS
  BRB studies were accomplished using dynamic contrast-enhanced MRI, and the retinal oxygenation response studies were accomplished by monitoring changes in the MRI signal associated with hyperoxic provocation. Participants were patients with diabetes and macular edema, with either no detectable or mild to moderate background retinopathy, as well as non-diabetic individuals of similar age (controls). Single-slice FLASH images were obtained using a Siemens Sonata, 1.5 T together with a Siemens 'Loop Small' surface coil fixed in place over the eye. Time-dependent changes in image contrast in the pre-retinal vitreous were quantified, and differences between patients and controls were assessed statistically.
  
  
  RESULTS
  The BRB breakdown studies showed a significant difference in the temporal evolution of the MRI signal enhancement post-contrast injection between the controls and the patients with diabetic macular edema. The retinal oxygenation studies revealed a supernormal oxygenation response in the pre-retinal vitreous in patients with diabetes who had no evidence of retinopathy, as well as in patients with background diabetic retinopathy. A nasal-temporal asymmetry in the evolution of retinal oxygenation response was found in patients with diabetes that was not present in healthy subjects.
  
  
  CONCLUSIONS
  These studies show that subtle differences in retinovascular function between patients with diabetes and non-diabetic individuals, including changes that occur in advance of the clinical appearance of diabetic retinopathy, can be detected with MRI. These results, together with previous extensive preclinical data, establish MRI as a powerful non-invasive method for measuring spatial and temporal changes in the same key retinovascular metrics in both animals and humans. Wide application of these techniques for diagnosis and evaluation of treatment efficacy in a variety of human retinopathies, including diabetic retinopathy, is expected.
authors.affiliation: Department of Ophthalmology, Henry Ford Health System, Detroit, MI 48202, USA. gltrick1@aol.com
authors.forename: Gary L
authors.initials: GL
authors.lastname: Trick
authors.forename: Paul A
authors.initials: PA
authors.lastname: Edwards
authors.forename: Uday
authors.initials: U
authors.lastname: Desai
authors.forename: Paula E
authors.initials: PE
authors.lastname: Morton
authors.forename: Zahid
authors.initials: Z
authors.lastname: Latif
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Berkowitz
mesh_terms: D000818:Animals; D001813:Blood-Retinal Barrier / Q000378:metabolism*; D003920:Diabetes Mellitus / Q000378:metabolism / Q000473:pathology; D003930:Diabetic Retinopathy / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008279:Magnetic Resonance Imaging; D010100:Oxygen / Q000378:metabolism; D012160:Retina / Q000033:anatomy & histology* / Q000473:pathology
chemical_list: D010100:Oxygen