Age-related difference of site-specific histone modifications in rat liver.
Aging is associated with decrease in activities of the transcription, replication and DNA repair that can result in deterioration of cellular and tissue functions. Changes of chromatin structures with age are likely major underling mechanisms for the functional decline. Chromatin consists of DNA and histones as well as non-histone proteins. While age-associated change of DNA methylation is well documented, little information is available on site-specific histone modifications in aging. We studied here age-related change of selected modifications of rat liver histone, i.e., histone H3 Lys9 acetylation (H3K9ac), H3 Lys9 methylation (H3K9me), H3 Ser10 phosphorylation (H3S10ph) and H3 Lys14 acetylation (H3K14ac). H3K9ac was decreased and H3S10ph was increased with age significantly. In view of reports indicating that decrease in acetylation and increase in phosphorylation of H3 histones can suppress gene activity, our findings suggest that a mechanism of decreased chromatin functions with age is due to such epigenetic changes.
Kawakami
Kyojiro
K
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba, Japan.
Nakamura
Akihiro
A
Ishigami
Akihito
A
Goto
Sataro
S
Takahashi
Ryoya
R
D000107:Acetylation; D000367:Age Factors; D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D042002:Chromatin Assembly and Disassembly; D044127:Epigenesis, Genetic; D006657:Histones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008239:Lysine; D008297:Male; D008745:Methylation; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D051381:Rats; D011916:Rats, Inbred F344; D012694:Serine
D006657:Histones; D012694:Serine; D008239:Lysine