[Effect of 2,4-dinitrophenol on the rat liver respiratory activity and ATP content after hypothermic storage and following reperfusion].
The influence of oxidative phosphorylation uncoupler 2,4-dinitrophenol (DNP) presence in preserving solution on the rat liver respiratory activity and ATP content after 18 h of hypothermic storage (HS) and following normothermic reperfusion (NR) was investigated. DNP presence on the HS stage led to decrease of ATP level as compared with the control. After DNP removal during NR the gradual recovery of oxidative phosphorylation coupling occurred. This fact resulted in improvement of mitochondrial functional state (V4 respiration rate decrease, respiratory control and ATP level increase).
Cherkashyna
D V
DV
Tkachova
O M
OM
Somov
O Iu
OIu
Semenchenko
O A
OA
Lebedyns'kyĭ
O S
OS
Petrenko
O Iu
OIu
D019297:2,4-Dinitrophenol / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D005260:Female; D007036:Hypothermia, Induced; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D015424:Reperfusion
D000255:Adenosine Triphosphate; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D019297:2,4-Dinitrophenol