title: Semliki Forest virus strongly reduces mosquito host defence signaling.
abstract: The Alphavirus genus within the Togaviridae family contains several important mosquito-borne arboviruses. Other than the antiviral activity of RNAi, relatively little is known about alphavirus interactions with insect cell defences. Here we show that Semliki Forest virus (SFV) infection of Aedes albopictus-derived U4.4 mosquito cells reduces cellular gene expression. Activation prior to SFV infection of pathways involving STAT/IMD, but not Toll signaling reduced subsequent virus gene expression and RNA levels. These pathways are therefore not only able to mediate protective responses against bacteria but also arboviruses. However, SFV infection of mosquito cells did not result in activation of any of these pathways and suppressed their subsequent activation by other stimuli.
authors.affiliation: The Roslin Institute & Royal (Dick) School of Veterinary Studies, College of Medicine & Veterinary Medicine, The University of Edinburgh, Scotland, UK.
authors.forename: R
authors.initials: R
authors.lastname: Fragkoudis
authors.forename: Y
authors.initials: Y
authors.lastname: Chi
authors.forename: R W C
authors.initials: RW
authors.lastname: Siu
authors.forename: G
authors.initials: G
authors.lastname: Barry
authors.forename: G
authors.initials: G
authors.lastname: Attarzadeh-Yazdi
authors.forename: A
authors.initials: A
authors.lastname: Merits
authors.forename: A A
authors.initials: AA
authors.lastname: Nash
authors.forename: J K
authors.initials: JK
authors.lastname: Fazakerley
authors.forename: A
authors.initials: A
authors.lastname: Kohl
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli; D005786:Gene Expression Regulation; D008156:Luciferases; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050791:STAT Transcription Factors / Q000378:metabolism; D012672:Semliki forest virus; D015398:Signal Transduction / Q000276:immunology*
chemical_list: D017931:DNA Primers; D050791:STAT Transcription Factors; D008156:Luciferases