title: [Establishment of a tight tetracycline-controlled HCV-C double transgenic mouse model].
abstract: OBJECTIVE
  To develop a tight tetracycline-controlled HCV-C double transgenic mouse model.
  
  
  METHODS
  By crossbreeding of ApoE-rtTA-tTS transgenic mice with TRE-HCV-C transgenic mice, the double transgenic mice were produced in the F1 generation. The presence of HCV-C and tTS gene in the F1 generation was confirmed by PCR, followed by further identification and quantification of the transgene using Southern blot hybridization. The expression of HCV-C in the liver of the mouse model was detected immunohistochemically.
  
  
  CONCLUSIONS
  Two transgenic mice were obtained, which contained ApoE-rtTA-tTS and TRE-HCV-C genes in the genome. Five founders contained HCV-C gene as confirmed by PCR and Southern blot hybridization. The tight tetracycline-controlled system may facilitate further study of HCV-C gene expression and gene therapy of hepatic cellular carcinoma.
authors.affiliation: Center for Disease Control and Prevention, Guangzhou, China.
authors.forename: Li-fang
authors.initials: LF
authors.lastname: Shuai
authors.forename: Bo-heng
authors.initials: BH
authors.lastname: Tang
authors.forename: Ruo-shuang
authors.initials: RS
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Guo-zhu
authors.initials: GZ
authors.lastname: Yang
authors.forename: Xi-gu
authors.initials: XG
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics*; D015139:Blotting, Southern; D001947:Breeding; D003433:Crosses, Genetic; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology; D018936:Hepatitis C Antigens / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction; D013752:Tetracycline / Q000494:pharmacology*; D015534:Trans-Activators / Q000235:genetics*; D014758:Viral Core Proteins / Q000235:genetics*
chemical_list: D001057:Apolipoproteins E; D018936:Hepatitis C Antigens; D015534:Trans-Activators; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus; D013752:Tetracycline