[Effect of rosiglitazone on tumor necrosis factor-alpha-induced nuclear factor-kappaB and coupling factor 6 expressions in human umbilical vein endothelial cells].
OBJECTIVE
To investigate the effect of rosiglitazone on the expression of nuclear factor-kappaB (NF-kappaB) and coupling factor 6 (CF6) induced by tumor necrosis factor-alpha (TNF-alpha) in cultured human umbilical vein endothelial cells (HUVEC).


METHODS
Cultured HUVEC of passage 3-5 were stimulated with TNF-alpha and then cultured in the presence of rosiglitazone. The expression of CF6 and NF-kappaB subunit p65 were evaluated by immunocytochemistical method.


RESULTS
Pretreatment of HUVECs with rosiglitazone inhibited TNF-alpha-induced expression of CF6 in a dose-dependent manner. The activation of CF6 stimulated by TNF-alpha was suppressed by ROS in a dose-dependent manner.


CONCLUSIONS
TNF-alpha-induced enhancement of the gene expression and release of CF6 is mediated by activation of NF-kappaB signaling pathway. ROS can inhibit the activation of IKK, block NF-kappaB signaling pathway and inhibit the expression of CF6, which may be the mechanism underlying the action of TZDs on hypertension.
Ye
Ze-bing
ZB
Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Li
Zhi-liang
ZL
Song
Shu-dong
SD
Pan
Di-guang
DG
Fu
Qiang
Q
Liu
Ying-feng
YF
D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007150:Immunohistochemistry; D025261:Mitochondrial Proton-Translocating ATPases / Q000096:biosynthesis*; D016328:NF-kappa B / Q000096:biosynthesis*; D010086:Oxidative Phosphorylation Coupling Factors / Q000096:biosynthesis*; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D007004:Hypoglycemic Agents; D016328:NF-kappa B; D010086:Oxidative Phosphorylation Coupling Factors; D045162:Thiazolidinediones; D014409:Tumor Necrosis Factor-alpha; D000077154:Rosiglitazone; C000628217:F(6) ATPase; D025261:Mitochondrial Proton-Translocating ATPases