title: Cytogenetic damage in preimplantation mouse embryos generated after paternal and parental gamma-irradiation and the influence of vitamin C.
abstract: Cytogenetic damage expressed as micronuclei (MN) in 4-8-cell embryos generated after irradiation of male or male and female mice in the absence and presence of vitamin C was investigated. Male NMRI mice were whole body exposed to 4 Gy gamma-rays and mated with non-irradiated superovulated female mice in 6 successive weeks after irradiation in a weekly interval. In experiments involving irradiation of both male and female mice, irradiated male mice for 6 weeks post irradiation were mated with female mice irradiated after induction of superovulation. Effect of 100 mg/kg vitamin C (ascorbic acid) on the frequency of MN was also studied. Pregnant animals were euthanized and embryos flushed from the oviducts and fixed on slides. The rate of MN observed in embryos generated from irradiated male compared with control group dramatically increased (P<0.01). Frequency of MN in this group decreased dramatically after vitamin C treatment (P<0.01). Frequency of MN in embryos generated by mating both male and female irradiated mice was higher than that observed for those embryos generated by irradiated male mice alone. However, a considerable modifying effect of vitamin C was observed for this group too (P<0.05). Results indicate that irradiation of gonads during spermatogenesis and preovulatory stage oocytes may lead to unstable chromosomal aberrations and probably stable chromosomal abnormalities affecting pairing and disjunction of chromosomes in successive preimplantation embryos expressed as MN. The way vitamin C reduces clastogenic effects of radiation on germ cells leading to reduced frequency of MN in pre-embryos might be due to its antioxidation and radical scavenging properties.
authors.affiliation: Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran. mozdarah@modares.ac.ir
authors.forename: Hossein
authors.initials: H
authors.lastname: Mozdarani
authors.forename: Elmina
authors.initials: E
authors.lastname: Nazari
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001755:Blastocyst / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D002869:Chromosome Aberrations; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D008297:Male; D018811:Maternal Exposure; D051379:Mice; D008815:Mice, Inbred Strains; D048629:Micronuclei, Chromosome-Defective; D018812:Paternal Exposure; D011247:Pregnancy; D013480:Superovulation; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D014815:Vitamins; D001205:Ascorbic Acid