First record of Perkinsus olseni, a protozoan parasite infecting the commercial clam Ruditapes decussatus in Spanish Mediterranean waters.
We present the first record in Spanish Mediterranean waters of the protozoan parasite Perkinsus olseni infecting the clam Ruditapes decussatus. Perkinsus infection was detected all year around albeit at a low level of infection intensity. Histological analysis, induction of zoospores and in situ hybridisation assay confirmed the presence of Perkinsus sp. The identity of the parasite was determined by species-specific PCR assay in DNA samples obtained from infected clams. Sequencing of amplified fragments showed 100% identity to the ITS region of P. olseni. We confirmed for the first time the presence of P. olseni in Spanish Mediterranean waters.
Elandaloussi
Laurence M
LM
IRTA-Sant Carles de la Ràpita, Sant Carles de la Ràpita, Spain. laurence.elandaloussi@irta.es
Carrasco
Noèlia
N
Roque
Ana
A
Andree
Karl
K
Furones
M Dolores
MD
D000818:Animals; D049872:Bivalvia / Q000166:cytology / Q000469:parasitology*; D008522:Mediterranean Sea; D010271:Parasites / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000737:chemistry; D017423:Sequence Analysis, RNA; D013030:Spain
D012335:RNA, Ribosomal