title: NMR metabolic profiling of organic and aqueous sea bass extracts: implications in the discrimination of wild and cultured sea bass.
abstract: The nuclear magnetic resonance (NMR) technique was used as analytical tool to determine the complete metabolic profiling of sea bass extracts: water-soluble metabolites belonging to different classes such as sugars, amino acids, dipeptides and organic acids as well as metabolites soluble in organic solvent such as lipids, sterols and fatty acids were identified. The metabolite profiling together with a suitable statistical analysis were used to discriminate between wild and cultured sea bass samples. Preliminary results show that discrimination between wild and cultured sea bass was obtained not only using fatty acid composition but also cholesterol and phosphatidylethanolamine and some water-soluble metabolites such as choline, trimethylamine oxide, glutamine, fumaric and malic acids.
authors.affiliation: Dipartimento STAAM, Università degli Studi del Molise, Via De Sanctis, 86100 Campobasso, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Mannina
authors.forename: A P
authors.initials: AP
authors.lastname: Sobolev
authors.forename: D
authors.initials: D
authors.lastname: Capitani
authors.forename: N
authors.initials: N
authors.lastname: Iaffaldano
authors.forename: M P
authors.initials: MP
authors.lastname: Rosato
authors.forename: P
authors.initials: P
authors.lastname: Ragni
authors.forename: A
authors.initials: A
authors.lastname: Reale
authors.forename: E
authors.initials: E
authors.lastname: Sorrentino
authors.forename: I
authors.initials: I
authors.lastname: D'Amico
authors.forename: R
authors.initials: R
authors.lastname: Coppola
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001492:Bass / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D005398:Fisheries; D050356:Lipid Metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010455:Peptides / Q000378:metabolism; D014020:Tissue Extracts / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D010455:Peptides; D014020:Tissue Extracts