Determination of protein and RNA expression levels of common housekeeping genes in a mouse model of neurodegeneration.
The choice of housekeeping proteins or genes for internal standards should be made carefully, taking into account the cell and tissue type, the experimental conditions, and the healthy/disease state(s) under consideration. Furthermore, as the correlation between transcriptional and translational levels of commonly used housekeeping genes is often discussed, this study shed light on the transcriptional levels of beta-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the translational levels of beta-actin, GAPDH, and beta-tubulin in an amyotrophic lateral sclerosis mouse model.
Calvo
Ana C
AC
LAGENBIO, University of Zaragoza, Zaragoza, Spain.
Moreno-Igoa
María
M
Manzano
Raquel
R
Ordovás
Laura
L
Yagüe
Gema
G
Oliván
Sara
S
Muñoz
María J
MJ
Zaragoza
Pilar
P
Osta
Rosario
R
D000199:Actins / Q000096:biosynthesis; D000690:Amyotrophic Lateral Sclerosis / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D004195:Disease Models, Animal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis; D051379:Mice; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D014158:Transcription, Genetic; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D011506:Proteins; D014404:Tubulin; D012313:RNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases