title: Electrochemical biosensor featuring a two-enzyme pathway and DNA for screening toxic reactive metabolites of arylamines.
abstract: We demonstrate for the first time a biosensor featuring a sequential two-enzyme pathway suitable to screen potentially toxic reactive metabolites generated during metabolism.
authors.affiliation: Department of Chemistry, University of Connecticut, USA.
authors.forename: Minjeong
authors.initials: M
authors.lastname: So
authors.forename: John B
authors.initials: JB
authors.lastname: Schenkman
authors.forename: James F
authors.initials: JF
authors.lastname: Rusling
mesh_terms: D000105:Acetyl Coenzyme A / Q000737:chemistry*; D000123:Acetyltransferases / Q000737:chemistry*; D015374:Biosensing Techniques; D002384:Catalysis; D019388:Cytochrome P-450 CYP1A2 / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D004563:Electrochemistry; D007093:Imidazoles / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity; D008567:Membranes, Artificial; D015394:Molecular Structure; D010084:Oxidation-Reduction; D013997:Time Factors
chemical_list: D007093:Imidazoles; D008567:Membranes, Artificial; D000105:Acetyl Coenzyme A; D004247:DNA; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; D019388:Cytochrome P-450 CYP1A2; D000123:Acetyltransferases