Functional implications of HNK-1 expression on invasive behaviour of melanoma cells.
HNK-1 epitope, also known as CD57, is expressed on a wide range of cells and has been related to cell to cell and cell to extracellular matrix interactions. Expression of the HNK-1 epitope has been considered a prognostic factor in several tumours, as it has been associated with the risk of metastasis. HNK-1 has been found to be expressed on uveal and cutaneous melanoma and proposed as a useful marker of the risk of metastasis. We have analysed the HNK-1 expression on a large panel of melanoma cell lines, the involvement of this epitope in melanoma cell adhesion, as well as its migrative and invasive behaviour. HNK-1 was highly expressed in 12.9% of melanoma cell lines, and in vitro experiments using invasive melanoma cell lines demonstrated that an HNK-1 blockade reduces cell adhesion to extracellular matrix as well as their migrative and invasive ability. These data support the functional relevance of HNK-1 expression in metastatic melanoma.
Casado
Javier G
JG
Department of Physiology, Immunology Unit, Faculty of Veterinary Science, University of Extremadura, Caceres, Spain. jgarcas@unex.es
Delgado
Elena
E
Patsavoudi
Evangelia
E
Durán
Esther
E
Sanchez-Correa
Beatriz
B
Morgado
Sara
S
Solana
Rafael
R
Tarazona
Raquel
R
D014408:Biomarkers, Tumor / Q000378:metabolism*; D018959:CD57 Antigens / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D010641:Phenotype; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D018959:CD57 Antigens