Comparative analysis of ESTs in response to drought stress in chickpea (C. arietinum L.).
Chickpea (Cicer arietinum L.) is an important pulse crop grown mainly in the arid and semi-arid regions. To identify the water-stress-induced genes, two non-normalized cDNA libraries were constructed from the seedling leaves of a drought-tolerant chickpea cultivar under PEG-treated and -nontreated conditions. About 2500 clones from each library were selected randomly for sequencing analysis. Based on IDEG6 online software analysis, 92 genes were differentially expressed, and these genes were involved in diverse biological progresses, such as metabolism, transcription, signal transduction, protein synthesis and others. Most of the up-regulated genes were related to drought tolerance, and the down-regulated genes were mainly involved in photosynthesis. The differential expression patterns of five functional unigenes were confirmed by quantitative real-time PCR (qPCR). The results will help in understanding the molecular basis of drought tolerance in chickpea.
Gao
Wen-Rui
WR
State Key Lab of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Department of Agriculture, Nanjing Agricultural University, Xuanwu District, Weigang No. 1, Nanjing 210095, China.
Wang
Xian-Sheng
XS
Liu
Qing-You
QY
Peng
Hui
H
Chen
Chen
C
Li
Jian-Gui
JG
Zhang
Ju-Song
JS
Hu
Song-Nian
SN
Ma
Hao
H
D029863:Cicer / Q000235:genetics* / Q000502:physiology; D016000:Cluster Analysis; D004190:Disasters; D015536:Down-Regulation; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D005810:Multigene Family; D010788:Photosynthesis / Q000235:genetics; D018526:Plant Transpiration / Q000235:genetics*; D015854:Up-Regulation; D014867:Water / Q000378:metabolism*
D014867:Water