title: A small-angle X-ray scattering study of the structure of lysozyme-sodium dodecyl sulfate complexes.
abstract: The structure of lysozyme-sodium dodecyl sulfate (SDS) complexes in solution is studied using small-angle X-ray scattering (SAXS). The SAXS data cannot be explained by the necklace and bead model for unfolded polypeptide chain interspersed with surfactant micelles. For the protein and surfactant concentrations used in the study, there is only marginal growth of SDS micelles as they complex with the protein. Being a small and rather rigid protein, lysozyme can penetrate the micellar core which is occupied by flexible and disordered paraffin chains and also the shell occupied by the hydrated head groups. A partially embedded swollen micellar model seems appropriate and describes well the scattering data. The SAXS intensity profiles are analyzed by considering the change in the electron scattering length density of the micellar core and shell due to complexation with protein and treating the intermicellar interaction using rescaled mean spherical approximation (RMSA) for charged spheres.
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
authors.forename: Janaky
authors.initials: J
authors.lastname: Narayanan
authors.forename: A S
authors.initials: AS
authors.lastname: Abdul Rasheed
authors.forename: Jayesh R
authors.initials: JR
authors.lastname: Bellare
mesh_terms: D000818:Animals; D002645:Chickens; D008823:Micelles; D009113:Muramidase / Q000737:chemistry*; D053838:Scattering, Small Angle; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D008823:Micelles; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D009113:Muramidase