Ability of deoxyribonucleic acid-damaged sperm to withstand freeze-thaw-induced damage during cryopreservation.
OBJECTIVE
To understand the association between sperm DNA damage and the ability of DNA-damaged sperm to withstand the freeze-thaw process during cryopreservation.


METHODS
Experimental prospective study.


METHODS
Embryology research laboratory.


METHODS
Eight- to 12-week-old Swiss strain male albino mice (Mus musculus).


METHODS
Sperm carrying a known amount of DNA damage was subjected to cryopreservation and thereafter evaluated for survival and freeze-thaw-induced DNA damage.


METHODS
Elucidation of association between the amount of initial sperm DNA damage, cryosurvival, and freeze-thaw-induced DNA modification.


RESULTS
A strong correlation (R = -0.87) was observed between the amount of initial sperm DNA damage and postthaw survival. However, no significant enhancement in DNA damage was observed by the cryopreservation of spermatozoa with various amounts of DNA damage.


CONCLUSIONS
Cryopreservation of DNA-damaged sperm does not deteriorate the DNA quality, but sperm survival is compromised.
Adiga
Satish Kumar
SK
Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India. Electronic address: satish.adiga@manipal.edu.
Khan
Zaheer
Z
Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.
Upadhya
Dinesh
D
Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.
Kalthur
Guruprasad
G
Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.
Kumar
Pratap
P
Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.
D000818:Animals; D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects; D015925:Cryopreservation; D004247:DNA / Q000502:physiology* / Q000528:radiation effects; D004249:DNA Damage / Q000502:physiology* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D008297:Male; D051379:Mice; D023421:Models, Animal; D011446:Prospective Studies; D012044:Regression Analysis; D012662:Semen Preservation / Q000009:adverse effects / Q000379:methods; D013094:Spermatozoa / Q000166:cytology* / Q000528:radiation effects; D013737:Testis / Q000502:physiology / Q000528:radiation effects
D004247:DNA