Development of a liquid chromatography-tandem mass spectrometry method for an euthanasic veterinarian drug: Tanax.
A development of a rapid and sensitive LC-MS/MS method for the simultaneous detection of active ingredients of the euthanasic veterinarian drug Tanax mixture is described. The method proposed, with a retention time of few minutes (6 min) was developed for an equine serum sample with solid-phase extraction (S.P.E). This S.P.E. procedure has been revealed useful for the determination of very low concentrations of Tanax analytes (0.05-1 ng/ml). The method was validated in terms of specificity/selectivity, sensitivity, recovery and precision.
Fidani
M
M
U.N.I.R.E. Lab. S.r.l., Via Gramsci 70, 20019 Settimo Milanese (MI), Italy.
Gamberini
M C
MC
Pasello
E
E
Palazzoli
F
F
Dimasi
T
T
Montana
M
M
D000577:Amides / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D002853:Chromatography, Liquid / Q000662:veterinary*; D003510:Cyclohexanes / Q000032:analysis / Q000633:toxicity; D004338:Drug Combinations; D004355:Drug Stability; D005065:Euthanasia; D017022:Flow Injection Analysis / Q000379:methods; D006736:Horses; D015394:Molecular Structure; D000644:Quaternary Ammonium Compounds / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000662:veterinary*; D013748:Tetracaine / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity*; D013997:Time Factors
D000577:Amides; D003510:Cyclohexanes; D004338:Drug Combinations; D000644:Quaternary Ammonium Compounds; C535255:mebenzonium iodide; D013748:Tetracaine; C059324:embutramide; C015591:T 61