Detection of mismatched DNAs via the binding affinity of MutS using a gold nanoparticle-based competitive colorimetric method.
A gold nanoparticle-based competitive colorimetric assay can detect mismatched DNAs using MutS, a mismatch binding protein, and determine their relative binding affinities for this protein by a simple color change and the melting temperature of DNA-functionalized nanoparticle assemblies.
Cho
Minseon
M
Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784, Republic of Korea. ciban@postech.ac.kr.
Han
Min Su
MS
Ban
Changill
C
D020137:Base Pair Mismatch; D001483:Base Sequence; D001667:Binding, Competitive; D003116:Color; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D006046:Gold / Q000737:chemistry / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051719:MutS DNA Mismatch-Binding Protein / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D011485:Protein Binding; D044366:Transition Temperature
D006046:Gold; D004247:DNA; D051719:MutS DNA Mismatch-Binding Protein