title: Is otospiralin inner ear specific? Evidence for its expression in mouse brain.
abstract: The small protein otospiralin has initially been identified as an inner ear specific molecule. However, compelling evidence from high throughput sequencing projects suggested that otospiralin is likely expressed in the central nervous system. Here, we tested this hypothesis using a combination of molecular biology, immunological, and histological techniques, and found that otospiralin is expressed in numerous regions of the central nervous system in mouse. In situ hybridization and immunohistochemistry revealed that otospiralin is widely expressed in neuronal cell bodies and glia. Ultrastructural observations in the cerebral cortex located the small protein in close proximity to membranous organelles in perikarya, the inner face of post-synaptic neuronal membranes, and in astrocytic processes. These results are in agreement with the predicted structure of the protein which revealed a single N-terminal transmembrane helix domain followed by a C-terminus cytosolic tail. Interestingly, 2 weeks after a mechanical trauma in the cerebral cortex, otospiralin expression increased in reactive astrocytes located within the vicinity of the site of injury, but not in neurons. Collectively, our observations suggest that otospiralin is possibly involved in signaling pathways, and could play a role in repair mechanisms subsequent to an injury in the central nervous system.
authors.affiliation: INSERM U587 and University of Bordeaux 2, Institut des Neurosciences de Bordeaux, Bordeaux F-33076, France.
authors.forename: Boris
authors.initials: B
authors.lastname: Decourt
authors.forename: Dean
authors.initials: D
authors.lastname: Hillman
authors.forename: Yohan
authors.initials: Y
authors.lastname: Bouleau
authors.forename: Didier
authors.initials: D
authors.lastname: Dulon
authors.forename: Aziz
authors.initials: A
authors.lastname: Hafidi
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism / Q000503:physiopathology; D005911:Gliosis / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D009416:Nerve Regeneration / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D017434:Protein Structure, Tertiary / Q000502:physiology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism / Q000648:ultrastructure
chemical_list: C503046:Otos protein, mouse; D011506:Proteins