On-chip screening method for cell migration genes based on a transfection microarray.
Cell migration plays a major role in a variety of biological processes and a detailed understanding of associated mechanisms should lead to advances in the medical sciences, for example, in drug discovery for cancer therapy. However, the traditional methods used for analysis of cell migration cannot easily be scaled up for high-throughput screening. In this study, we have attempted to develop a novel simple method for high-throughput phenotypic screening for the identification of genes that are required for cell migration. As the appropriate cell line for the method, we found NBT-L2b cells that would be suitable for screening of migration-related genes in our method without influence by other cellular processes. Moreover, the idea for printing both the labeled fibronectin, for identification of the starting region of a cell, and the green fluorescent protein (GFP) expression vector, for identification of cells that had been transfected with siRNA and of the end point of migration, brings a rapid and efficient high-throughput screening procedure. Our new method will lead to an enhanced understanding of cell migration.
Onuki-Nagasaki
Reiko
R
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tokyo, 135-0064, Japan.
Nagasaki
Akira
A
Hakamada
Kazumi
K
Uyeda
Taro Q P
TQ
Fujita
Satoshi
S
Miyake
Masato
M
Miyake
Jun
J
D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010641:Phenotype; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D019076:Transgenes / Q000235:genetics*
D034741:RNA, Small Interfering