Curbing rampant cross-contamination and misidentification of cell lines.
A son's challenge started an emeritus professor of biology on a three-year odyssey to get biological researchers to correct a decades-long problem with cross-contaminated and misidentified cell lines. These errors may account for more than 15% of mammalian cultures, wasting resources and undermining the integrity of research.
Nardone
Roland M
RM
Department of Biology, Catholic University of America, Washington, DC, USA. nardone@cua.edu
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods / Q000592:standards*; D002460:Cell Line; D019070:Cell Lineage; D002478:Cells, Cultured; D016172:DNA Fingerprinting / Q000191:economics / Q000379:methods; D004273:DNA, Neoplasm / Q000032:analysis; D020869:Gene Expression Profiling; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007621:Karyotyping; D015203:Reproducibility of Results; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D007527:Isoenzymes