[Influence of products of bacterial origin on the expression of surface molecules in monocyte-derived and endothelial cells].
OBJECTIVE
To study the influence of lypopolysaccharide (LPS) of Gram-negative bacterium (Escherichia coli O55:B5) and lysate of Gram-positive bacteria (Streptococcus pyogenes - group A, type M1, strain 40/58) on the level of expression of important surface molecules of monocyte-derived cells from continuous cell line THP-1 and endothelial cells from continuous cell line EA.hy 926.


METHODS
Expression of surface molecules HLA-DR, CD11b, CD14, CD16, CD32, and CD54 was assessed using FITC- or PE-labeled monoclonal antibodies (Beckman Coulter, USA). Intensity of fluorescence was measured by flow cytometer Epics Altra manufactured by Beckman Coulter (USA).


RESULTS
Studied components of Gram-positive and Gram-negative bacteria stimulated expression of CD14, CD16, CD32, and CD54 molecules on cells from THP-1 line; incubation of cells from EA.hy 926 line in the presence of the same bacterial components increased expression levels of CD54 and HLA-DR molecules.


CONCLUSIONS
Endothelial cells of EA.hy 926 line was less sensitive to LPS of E. coli and lysate of S. pyogenes compared to monocyte-derived cells of THP-1 line. Usage of THP-1 cells allowed to reveal differences between effects of components of Gram-positive and Gram-negative bacteria. The stimulating effect of LPS was more pronounced compared to effect of S. pyogenes lysate in relation to expression of HLA-DR, CD11b, and CD54 molecules, whereas lysate of S. pyogenes better stimulated expression of CD14, CD16, and CD32 molecules.
Chernova
A A
AA
Starikova
E A
EA
Sokolov
D I
DI
Burova
L A
LA
Freĭdlin
I S
IS
D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology*; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D006684:HLA-DR Antigens / Q000096:biosynthesis / Q000276:immunology*; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology* / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D013045:Species Specificity; D013297:Streptococcus pyogenes / Q000276:immunology
D015703:Antigens, CD; D006684:HLA-DR Antigens; D008070:Lipopolysaccharides