Neutravidin micropatterning by deep UV irradiation.
We describe a novel approach for directly patterning neutravidin protein by exposure to deep UV irradiation. Neutravidin is physically absorbed onto the glass or quartz substrate, dehydrated in acetone and air-dried. Dry neutravidin-coated samples are patterned either by top-side or back-side exposure to 185 nm UV. Subsequent introduction of fluorescent biotinylated proteins clearly demonstrates binding to the masked neutravidin regions and no binding to exposed areas. Patterned samples retain their protein affinity for at least three months of storage at room temperature.
Huang
Ying-Ming
YM
Department of Electrical Engineering, Penn State University, 218 Electrical Engineering West Building, University Park, PA 16803, USA.
Uppalapati
Maruti
M
Hancock
William Olaf
WO
Jackson
Thomas Nelson
TN
D001360:Avidin / Q000737:chemistry*; D014466:Ultraviolet Rays
C093483:neutravidin; D001360:Avidin