Helicobacter pylori neutrophil-activating protein promotes myeloperoxidase release from human neutrophils.
Helicobacter pylori infection induces acute and chronic inflammation and plays a key role in gastric mucosal diseases. H. pylori neutrophil-activating protein (HP-NAP), one of its virulence factors, induces not only chemotactic but also oxidative burst responses of neutrophils. Activated neutrophils use myeloperoxidase (MPO) to generate many cytotoxic oxidants, which might result in gastric mucosal injury. In this study, we evaluated whether HP-NAP could promote MPO release from human neutrophils. Recombinant HP-NAP expressed in Escherichia coli was purified by two sequential gel filtration chromatographies and then subjected to syringe filtration for endotoxin removal. The purified recombinant HP-NAP assembles into a multimer with a secondary structure of the typical alpha-helix. In addition to stimulating the production of reactive oxygen species, HP-NAP is able to induce the secretion of MPO in human neutrophils. The increased MPO release from neutrophils induced by HP-NAP may be related to the pathogenesis of H. pylori-associated gastritis.
Wang
Chung-An
CA
Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan, ROC.
Liu
Yen-Chun
YC
Du
Shin-Yi
SY
Lin
Chio-Wen
CW
Fu
Hua-Wen
HW
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D004926:Escherichia coli / Q000235:genetics; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D009195:Peroxidase / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology
D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; C092412:neutrophil-activating protein A, Helicobacter pylori; D009195:Peroxidase