title: Plasmodium vivax circumsporozoite variants and Duffy blood group genotypes in the Brazilian Amazon region.
abstract: The circumsporozoite protein (CSP) of the Plasmodium vivax infective sporozoite is considered to be a major target for the development of recombinant malaria vaccines. The Duffy blood group molecule acts as the red blood cell receptor for P. vivax. We review the frequency of P. vivax CSP variants and report their association with the Duffy blood group genotypes from Brazilian Amazon patients carrying P. vivax malaria. Peripheral blood samples were collected from 155 P. vivax-infected individuals from five Brazilian malaria-endemic areas. The P. vivax CSP variants and the Duffy blood group genotypes were assessed using PCR/RFLP. In single infections, the VK210 variant was the commonest followed by the P. vivax-like variant. The typing of P. vivax indicated that the frequency of variants among the study areas was significantly different from one to another. This is the first detection of the VK247 and P. vivax-like variant in single infections in endemic areas of Brazil. Association of the CSP P. vivax variants with the heterozygous Duffy blood group system genotype was significant for VK210 single infection. These observations provide additional data on the Plasmodium-host interactions concerning the Duffy blood group and P. vivax capability of causing human malaria.
authors.affiliation: University of São Paulo State Júlio Mesquita Filho, São José do Rio Preto, São Paulo State, Brazil.
authors.forename: Luciane M
authors.initials: LM
authors.lastname: Storti-Melo
authors.forename: Wanessa C
authors.initials: WC
authors.lastname: de Souza-Neiras
authors.forename: Gustavo C
authors.initials: GC
authors.lastname: Cassiano
authors.forename: Ana C P
authors.initials: AC
authors.lastname: Joazeiro
authors.forename: Cor J
authors.initials: CJ
authors.lastname: Fontes
authors.forename: Cláudia R
authors.initials: CR
authors.lastname: Bonini-Domingos
authors.forename: Alvaro A R D'Almeida
authors.initials: AA
authors.lastname: Couto
authors.forename: Marinete M
authors.initials: MM
authors.lastname: Povoa
authors.forename: Luiz C
authors.initials: LC
authors.lastname: de Mattos
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Cavasini
authors.forename: Andréa R B
authors.initials: AR
authors.lastname: Rossit
authors.forename: Ricardo L D
authors.initials: RL
authors.lastname: Machado
mesh_terms: D000818:Animals; D001938:Brazil; D004375:Duffy Blood-Group System / Q000235:genetics*; D005260:Female; D014644:Genetic Variation / Q000502:physiology; D005838:Genotype; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood*; D008297:Male; D010966:Plasmodium vivax; D016133:Polymerase Chain Reaction / Q000379:methods; D015800:Protozoan Proteins / Q000097:blood* / Q000145:classification
chemical_list: D004375:Duffy Blood-Group System; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan