High-performance liquid chromatography determination of nitrated polycyclic aromatic hydrocarbons by indirect fluorescence detection.
A high-performance liquid chromatography (HPLC) method for the analysis of nitrated polcyclic aromatic hydrocarbons (NPAHs) is reported. NPAH mixtures were pre-concentrated using solid-phase extraction and well resolved on a C(18) column. They were detected using an indirect method involving the quenching of the emission from the fluorophores 5,6,7,8-tetrahydronaphthol (5,6,7,8-THN-1-OH), 7-amino-4-methyl coumarin (Coumarin 120, COU-120) and 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthylazo)2-naphthalene carboxylic acid (Calcon carboxylic acid, CCA). Linear calibration curves were obtained in the range 1.1 x 10(-9) to 1.1 x 10(-8) mol/L. Using COU 120 as the fluorophore, the detection limit was 2.9 x 10(-10) mol/L for 1-nitronaphthalene and 2.1 x 10(-11) mol/L for 2-nitrofluorene. Recoveries of NPAHs from spiked tap water samples were between 88 and 100%.
Al-Kindy
Salma M
SM
Department of Chemistry College of Science, Sultan Qaboos University PO Box 36, Al-Khod 123, Sultanate of Oman. alkindy@squ.edu.om
Miller
James N
JN
D001391:Azo Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D016014:Linear Models; D009284:Naphthols / Q000737:chemistry; D009574:Nitro Compounds / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D014867:Water / Q000737:chemistry
D001391:Azo Compounds; D003374:Coumarins; D005456:Fluorescent Dyes; D009284:Naphthols; D009574:Nitro Compounds; D011084:Polycyclic Aromatic Hydrocarbons; D014867:Water; C084567:calconcarboxylic acid; C028743:7-amino-4-methylcoumarin