title: Reversible pH lability of cross-linked vault nanocapsules.
abstract: Vaults are ubiquitous, self-assembled protein nanocapsules with dimension in the sub-100 nm range that are conserved across diverse phyla from worms to humans. Their normal presence in humans at a copy number of over 10,000/cell makes them attractive as potential drug delivery vehicles. Toward this goal, bifunctional amine-reactive reagents are shown to be useful for the reversible cross-linking of recombinant vaults such that they may be closed and opened in a controllable manner.
authors.affiliation: Chemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Marcella
authors.initials: M
authors.lastname: Yu
authors.forename: Benny C
authors.initials: BC
authors.lastname: Ng
authors.forename: Leonard H
authors.initials: LH
authors.lastname: Rome
authors.forename: Sarah H
authors.initials: SH
authors.lastname: Tolbert
authors.forename: Harold G
authors.initials: HG
authors.lastname: Monbouquette
mesh_terms: D000588:Amines / Q000737:chemistry; D000818:Animals; D001709:Biotechnology / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D016503:Drug Delivery Systems; D004583:Electrons; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D008968:Molecular Conformation; D053769:Nanocapsules / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*
chemical_list: D000588:Amines; D003432:Cross-Linking Reagents; D053769:Nanocapsules