Pholasin luminescence is enhanced by addition of dehydrocoelenterazine.
Pholasin is a bioluminescent photoprotein of Pholas dactylus. Pholasin is a commercially available photoprotein used to measure intracellular reactive oxygen species. Although extensive efforts have been carried out to determine the chemical structure of the prosthetic (chromophore) group, it still remains unclear to date. Herein, we report the enhancement of pholasin luminescence by the addition of dehydrocoelenterazine, which is organic substance of luminous squids' photoprotein.
Kuse
Masaki
M
Chemical Instrument division, Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan. kuse@cic.nagoya-u.ac.jp
Tanaka
Eiko
E
Nishikawa
Toshio
T
D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods; D049832:Decapodiformes; D015195:Drug Design; D049454:Firefly Luciferin / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry* / Q000494:pharmacology; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry* / Q000494:pharmacology; D008163:Luminescent Measurements; D008164:Luminescent Proteins; D008956:Models, Chemical; D011719:Pyrazines / Q000737:chemistry* / Q000494:pharmacology; D017382:Reactive Oxygen Species; D013997:Time Factors
D007093:Imidazoles; D049408:Luminescent Agents; D008164:Luminescent Proteins; D011719:Pyrazines; D017382:Reactive Oxygen Species; C116816:dehydrocoelenterazine; D049454:Firefly Luciferin; C052157:pholasin