D-Glucose sensing by a plasma membrane regulator of G signaling protein, AtRGS1.
Plants use sugars as signaling molecules and possess mechanisms to detect and respond to changes in sugar availability, ranging from the level of secondary signaling molecules to altered gene transcription. G-protein-coupled pathways are involved in sugar signaling in plants. The Arabidopsis thaliana regulator of G-protein signaling protein 1 (AtRGS1) combines a receptor-like seven transmembrane domain with an RGS domain, interacts with the Arabidopsis Galpha subunit (AtGPA1) in a d-glucose-regulated manner, and stimulates AtGPA1 GTPase activity. We determined that AtRGS1 interacts with additional components, genetically defined here, to serve as a plasma membrane sensor for d-glucose. This interaction between AtRGS1 and AtGPA1 involves, in part, the seven-transmembrane domain of AtRGS1.
Grigston
Jeffrey C
JC
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA.
Osuna
Daniel
D
Scheible
Wolf-Rüdiger
WR
Liu
Chenggang
C
Stitt
Mark
M
Jones
Alan M
AM
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D020710:RGS Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; C429765:GPA1 protein, Arabidopsis; D044385:GTP-Binding Protein alpha Subunits; D008565:Membrane Proteins; D020710:RGS Proteins; C477755:RGS1 protein, Arabidopsis; C512069:THF1 protein, Arabidopsis; D005947:Glucose