title: The predictive value of CD38 positive hepatic stellate cell count for assessing disease activity and fibrosis in patients with chronic hepatitis.
abstract: The activation of hepatic stellate cells (HSCs) is a critical event in hepatic fibrosis. The objectives of this study were to find out if cluster of differentiation 38 (CD38) can be demonstrated immunohistochemically on HSCs in liver biopsies from patients with chronic liver disease and if CD38 immunopositive HSC count is correlated with METAVIR inflammatory and fibrosis scores. Immunohistochemical labelling for CD38 was performed on 100 liver biopsies from patients with chronic liver disease. The CD38 immunopositive HSCs were identified and counted. The CD38 immunopositive HSC count was found to be associated with both the METAVIR score and the fibrosis scores. The CD38 immunopositive HSC count was able to discriminate between no fibrosis and stages 2, 3 or 4 fibrosis, but could not discriminate between no fibrosis and stage 1 fibrosis. Using receiver operating characteristic (ROC) curves, a cut-off point of 10 HSCs per 10 high power field (hpf), or 25 per 100 hepatocytes, is 80% sensitive and 70% specific for predicting fibrosis. The specificity rose to 100% in patients with hepatitis C viral (HCV) infection. We conclude that CD38 positive HSCs can be demonstrated immunohistochemically and that the count is highly predictive of moderate to severe hepatic fibrosis.
authors.affiliation: Department of Pathology, Faculty of Medicine, Health Science Centre, Kuwait University, P.O. Box. 24923, 13110 Safat, Kuwait. suadabdeen@hsc.edu.kw
authors.forename: Suad M
authors.initials: SM
authors.lastname: Abdeen
authors.forename: Samuel Olusoji
authors.initials: SO
authors.lastname: Olusi
authors.forename: Hifa A
authors.initials: HA
authors.lastname: Askar
authors.forename: Lukman
authors.initials: L
authors.lastname: Thalib
authors.forename: Aysha
authors.initials: A
authors.lastname: Al-Azemi
authors.forename: Sunila
authors.initials: S
authors.lastname: George
mesh_terms: D051997:ADP-ribosyl Cyclase 1; D000328:Adult; D005260:Female; D005355:Fibrosis / Q000175:diagnosis* / Q000209:etiology*; D055166:Hepatic Stellate Cells / Q000166:cytology* / Q000276:immunology*; D006521:Hepatitis, Chronic / Q000150:complications* / Q000175:diagnosis*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests
chemical_list: D051997:ADP-ribosyl Cyclase 1