Epigenetic deregulation of the human Oct4 promoter in mouse cells.
To examine whether the epigenetic status of the human Oct4 promoter is similarly regulated in mouse cells, we engineered a human bacterial artificial chromosome to express green fluorescent protein under the control of the hOct4 promoter and stably integrated it into mouse embryonic stem cells (mESCs), NIH3T3, and 293T cells. The hOct4 promoter is unmethylated in mESCs and it undergoes methylation during retinoic acid-induced differentiation. However, the hOct4 promoter is demethylated in NIH3T3 cells even though it is fully methylated in 293T cells. Methylation status of the hOct4 promoter is associated with green fluorescent protein expression at transcription level. Our findings indicate that the hOct4 promoter is differently regulated in mouse cells.
Cha
Young
Y
Graduate School of Life Science and Biotechnology, CHA Research Institute, College of Medicine, Seoul, South Korea.
Sung
Min-Kyung
MK
Jung
Kyung-Won
KW
Kim
Hwan-Hee
HH
Lee
Su-Man
SM
Park
Kyung-Soon
KS
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D018507:Gene Expression Regulation, Developmental; D005818:Genetic Engineering; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D050814:Octamer Transcription Factor-3 / Q000235:genetics*; D009928:Organ Specificity; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*
D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D049452:Green Fluorescent Proteins