Effect of porcine brain growth factor on primary cell cultures and BHK-21 [C-13] cell line.
Growth factors from neural tissues have been described as potent mitogens for a wide variety of mesoderm- and ectoderm-derived cells in vitro. We used porcine brain extract for in vitro testing of proliferation properties on primary ovarian cells, uterine cells, and cardiomyocytes in culture as well as for BHK-21 [C-13] cell line. The addition of this extract accelerates proliferation in all examined cultures. It also lowers serum requirement and shortens the cultivation period for BHK-21 [C-13] cells. Fibroblast growth factors from brain of different species, but not porcine, are already characterized and their proliferative effect proved. Therefore, we purified, determined, and confirmed the presence of basic fibroblast growth factor in porcine brain extract by Western blot analysis and showed its biological activity on BHK-21 [C-13] cells.
Gaurina Srcek
Visnja
V
Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotti St. 6, 10000 Zagreb, Croatia.
Radosević
Kristina
K
Kniewald
Hrvoje
H
Slivac
Igor
I
Kmetic
Ivana
I
Kniewald
Zlatko
Z
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D013552:Swine
D036341:Intercellular Signaling Peptides and Proteins