title: Analysis of donor- and isolation-related variables from non-heart-beating donors (NHBDs) using the Kyoto islet isolation method.
abstract: Recently, we demonstrated that islet transplantation from non-heart-beating donors (NHBDs) using the Kyoto islet isolation method (KIIM) successfully reversed patients' diabetes state. In this study, we evaluated the effects of donor- and isolation-related variables on islet isolation results from NHBDs by KIIM. Twenty-one islet preparations from the pancreata of NHBDs were isolated by KIIM. Islet preparations that met transplantation criteria and achieved improved patient diabetes control after transplantation were defined as successful isolations. Potential risk factors deemed to affect islet isolation results, such as age, gender, body mass index, hospital stay, donors' blood biochemical tests, a modified pancreata procurement method, and isolation and purification procedure-related variables, were analyzed. Seventeen out of 21 islet isolations (81%) were successful isolations. Postpurification islet yield was 447,639 +/- 39,902 islet equivalents (IE) in the successful isolation group and 108,007 +/- 31,532 IE in the failure group. Donor age was significantly younger in the success group (41.9 +/- 4.0 years old in the success group vs. 57.5 +/- 2.2 years old in the failure group, p = 0.003). Chronic pancreatitis significantly decreased islet yields (p = 0.006). Phase I time was significantly shorter (p = 0.010) and undigested tissue volume was significantly smaller (p = 0.020) in the success group. Purity was in positive correlation to postpurification islet yield, while donor age was in reverse correlation to postpurification islet yield. KIIM enables us to perform islet transplantation from NHBDs; however, the decision to use pancreata from older donors or those with chronic pancreatitis requires careful consideration.
authors.affiliation: General Surgery, First Clinical College of Harbin Medical University, Harbin, PR China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.forename: Shinichi
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Teru
authors.initials: T
authors.lastname: Okitsu
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Iwanaga
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Noguchi
authors.forename: Yukihide
authors.initials: Y
authors.lastname: Yonekawa
authors.forename: Hideo
authors.initials: H
authors.lastname: Nagata
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kamiya
authors.forename: Michiko
authors.initials: M
authors.lastname: Ueda
authors.forename: Nobuyo
authors.initials: N
authors.lastname: Hatanaka
authors.forename: Shuichi
authors.initials: S
authors.lastname: Miyakawa
authors.forename: Naoya
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Chunfang
authors.initials: C
authors.lastname: Song
mesh_terms: D000328:Adult; D015992:Body Mass Index; D001835:Body Weight; D002469:Cell Separation / Q000379:methods; D003922:Diabetes Mellitus, Type 1 / Q000628:therapy*; D005260:Female; D006801:Humans; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000379:methods*