ROCKs are expressed in brain tumors and are required for glioma-cell migration on myelinated axons.
The interactions between migrating glioma cells and myelinated fiber tracts are poorly understood. We identified that C6 glioma cells can migrate along myelinated chicken retinal axons in a novel coculture, thereby expressing small GTPases of the Rho family and serine/threonine Rho-associated kinases (ROCKs). We found that the ROCK1 isoform is also highly expressed in native human high-grade gliomas. Glioma cells migrated faster in vitro along myelinated axons than on laminin-1, with the former but not the latter being specifically and reversibly blocked by the ROCK inhibitor Y27632. These data suggest that the mechanisms underlying the migration of glioma cells on myelinated axons differ from those underlying the migration on extracellular matrix molecules such as laminin-1.
Oellers
Patrick
P
Department of Experimental Ophthalmology, School of Medicine, University Eye Hospital Münster, Domagkstrasse 15, Münster, Germany.
Schröer
Uwe
U
Senner
Volker
V
Paulus
Werner
W
Thanos
Solon
S
D000199:Actins / Q000378:metabolism; D000577:Amides / Q000494:pharmacology; D000818:Animals; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D001932:Brain Neoplasms / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology*; D002465:Cell Movement; D002642:Chick Embryo; D018920:Coculture Techniques; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005910:Glioma / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D007797:Laminin / Q000378:metabolism; D009367:Neoplasm Staging; D009413:Nerve Fibers, Myelinated / Q000502:physiology* / Q000648:ultrastructure; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D012160:Retina / Q000166:cytology; D020741:rho GTP-Binding Proteins / Q000378:metabolism; D054460:rho-Associated Kinases / Q000378:metabolism*
D000199:Actins; D000577:Amides; D004791:Enzyme Inhibitors; D007797:Laminin; D011725:Pyridines; C121346:laminin 1; C108830:Y 27632; D054460:rho-Associated Kinases; D020741:rho GTP-Binding Proteins