Transcriptional profiling of the PDR gene family in rice roots in response to plant growth regulators, redox perturbations and weak organic acid stresses.
The role of plant pleiotropic drug resistance (PDR) type ATP-binding cassette (ABC) transporters remains poorly understood. We characterized the expression of the rice pleiotropic drug resistance (PDR) gene family in roots, where PDR transporters are believed to have major functions. A prototypical oligonucleotide array was developed containing 70-mers chosen in the gene-specific 3' untranslated regions of the rice PDR genes, other full-molecule rice ABC transporter genes and relevant marker genes. Jasmonates, which are involved in plant defense and secondary metabolism, proved major inducers of PDR gene expression. Over half of the PDR genes were JA-induced in roots of rice; OsPDR9 to the highest level. Salicylic acid, involved in plant pathogen defense, markedly induced the expression of OsPDR20. OsPDR20 was cDNA cloned and characterized. Abscisic acid, typically involved in water deficit responses, particularly induced OsPDR3 in roots and shoot and OsPDR6 in rice leaves. OsPDR9 and OsPDR20 were furthermore up-regulated in response to dithiothreitol- or glutathione-induced redox perturbations. Exogenous application of the weak organic acids lactic acid, malic acid, and citric acid differentially induced the expression of OsPDR3, OsPDR8, OsPDR9 and OsPDR20 in rice seedling roots. This transcriptional survey represents a guide for the further functional analysis of individual PDR transporters in roots of rice.
Moons
Ann
A
Montreal General Hospital, 1650 Cedar Ave, L5-312, Montreal, QC H3G 1A4, Canada. ann.moons@mail.mcgill.ca
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000040:Abscisic Acid / Q000494:pharmacology; D000143:Acids / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology; D003583:Cytokinins / Q000494:pharmacology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000187:drug effects / Q000235:genetics*; D010084:Oxidation-Reduction / Q000187:drug effects; D054883:Oxylipins / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics*; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics; D020156:Salicylic Acid / Q000494:pharmacology; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D013312:Stress, Physiological / Q000187:drug effects; D013997:Time Factors
D018528:ATP-Binding Cassette Transporters; D000143:Acids; D003517:Cyclopentanes; D003583:Cytokinins; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C011006:jasmonic acid; D000040:Abscisic Acid; D020156:Salicylic Acid