Probing osmotic effects on invertase with L-(-)-sucrose.
L-(-)-sucrose was efficiently synthesized using intramolecular aglycon delivery and used to elucidate osmotic effects on the activity of invertase, which catalyzes the hydrolysis of D-(+)-sucrose. The osmotic effect imposed by L-sucrose was responsible for more than 30% of the activity loss ascribed otherwise to "substrate inhibition."
Seo
Seung-kee
SK
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907-2084, USA.
Wei
Alexander
A
D008954:Models, Biological; D009995:Osmosis; D013379:Substrate Specificity; D013395:Sucrose / Q000737:chemistry*; D043324:beta-Fructofuranosidase / Q000737:chemistry* / Q000378:metabolism
D013395:Sucrose; D043324:beta-Fructofuranosidase