Development of a photosystem II-based optical microfluidic sensor for herbicide detection.
Herbicides are highly toxic for both human and animal health. The increased application of herbicides in agriculture during the last decades has resulted in the contamination of both soil and water. Herbicides, under illumination, can inhibit photosystem II electron transfer. Photosynthetic membranes isolated from higher plants and photosynthetic micro-organisms, immobilized and stabilized, can serve as a biorecognition element for a biosensor. The inhibition of photosystem II causes a reduced photoinduced production of hydrogen peroxide, which can be measured by a chemiluminescence reaction with luminol and the enzyme horseradish peroxidase. In the present work, a compact and portable sensing device that combines the production and detection of hydrogen peroxide in a single flow assay is proposed for herbicide detection.
Varsamis
Dimitrios G
DG
Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK.
Touloupakis
Eleftherios
E
Morlacchi
Pietro
P
Ghanotakis
Demetrios F
DF
Giardi
Maria Teresa
MT
Cullen
David C
DC
D006540:Herbicides / Q000032:analysis* / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008280:Magnetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D018724:Spinacia oleracea / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism
D006540:Herbicides; D045332:Photosystem II Protein Complex; D006861:Hydrogen Peroxide