title: Impaired intestinal barrier integrity in the colon of patients with irritable bowel syndrome: involvement of soluble mediators.
abstract: BACKGROUND
  Growing evidence suggests that patients with irritable bowel syndrome (IBS) have increased intestinal permeability. In addition, mucosal soluble mediators are involved in the pathophysiology of pain in IBS. We aimed to investigate (1) paracellular permeability in colonic biopsies of patients with IBS; and (2) the ability of soluble factors from colonic biopsies to reproduce these alterations in vitro.
  
  
  METHODS
  Paracellular permeability in colonic biopsies of healthy subjects and patients with IBS was measured by mounting the biopsies in Ussing chambers. Cleared supernatant (SUP) of the culture from colonic biopsies was collected and applied to Caco-2 cells for 48 h. Paracellular permeability and transepithelial resistance (TER) were evaluated. mRNA expression of the tight junction proteins, zonula occludens (ZO)-1 and occludin, was assessed in colonic biopsies. Abdominal pain was assessed using a validated questionnaire.
  
  
  RESULTS
  Permeability of colonic biopsies was significantly higher in patients with IBS compared to healthy subjects. These changes were associated with significantly lower expression of ZO-1 mRNA in biopsies of IBS as compared to healthy subjects. Compared to healthy subjects, SUP of IBS markedly reduced TER and significantly increased permeability in Caco-2 cells. SUP of IBS patients induced a significant decrease of ZO-1 mRNA in Caco-2 as compared to healthy subjects. SUP-induced increased paracellular permeability correlated with the severity of abdominal pain.
  
  
  CONCLUSIONS
  Our study shows that colonic soluble mediators are able to reproduce functional (permeability) and molecular (ZO-1 mRNA expression) alterations observed in IBS patients. These findings might pave the way both to identify novel biomarkers as well as new therapeutic targets in IBS.
authors.affiliation: INSERM U913, Nantes, France.
authors.forename: T
authors.initials: T
authors.lastname: Piche
authors.forename: G
authors.initials: G
authors.lastname: Barbara
authors.forename: P
authors.initials: P
authors.lastname: Aubert
authors.forename: S
authors.initials: S
authors.lastname: Bruley des Varannes
authors.forename: R
authors.initials: R
authors.lastname: Dainese
authors.forename: J L
authors.initials: JL
authors.lastname: Nano
authors.forename: C
authors.initials: C
authors.lastname: Cremon
authors.forename: V
authors.initials: V
authors.lastname: Stanghellini
authors.forename: R
authors.initials: R
authors.lastname: De Giorgio
authors.forename: J P
authors.initials: JP
authors.lastname: Galmiche
authors.forename: M
authors.initials: M
authors.lastname: Neunlist
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001706:Biopsy; D018938:Caco-2 Cells; D016022:Case-Control Studies; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability; D003106:Colon; D017097:Electric Impedance; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D043183:Irritable Bowel Syndrome / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008875:Middle Aged; D062793:Occludin; D010750:Phosphoproteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D055815:Young Adult; D062826:Zonula Occludens-1 Protein
chemical_list: D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; D010750:Phosphoproteins; D012333:RNA, Messenger; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein