Dynamic alterations of cerebral pial microcirculation during experimental subarachnoid hemorrhage.
The study aimed to investigate the involvement of cerebral microcirculation turbulence after subarachnoid hemorrhage (SAH). Wistar rats were divided into non-SAH and SAH groups. Autologous arterial hemolysate was injected into rat's cisterna magna to induce SAH. Changes of pial microcirculation within 2 h were observed. It was found that there were no obvious changes of the diameters, flow velocity, and fluid state of microvessels in non-SAH group. With the exception of rare linear-granular flow in A4 arteriole, linear flow was observed in most of the arterioles. There was no blood agglutination in any of the arterioles. After SAH, abnormal cerebral pial microcirculation was found. Spasm of microvessels, decreased blood flow, and agglutination of red blood cells occurred. Five minutes following the induction of SAH, the diameters of the arterioles and venules significantly decreased. The decreased diameters persisted for 2 h after cisternal injection. Decreased flow velocity of venules was found from 5 to 90 min after induction of SAH. Spasm of the basilar artery and increased brain malondialdehyde were also found after SAH. We concluded that cerebral microcirculation turbulence plays an important role in the development of secondary cerebral ischemia following SAH.
Sun
Bao-Liang
BL
Department of Neurology & Institute of Microcirculation, Affiliated Hospital, Taishan Medical College, No.706, Taishan Avenue, Taian, Shandong 271000, China. blsun@tsmc.edu.cn
Zheng
Cheng-Bi
CB
Yang
Ming-Feng
MF
Yuan
Hui
H
Zhang
Su-Ming
SM
Wang
Le-Xin
LX
D000818:Animals; D001160:Arterioles / Q000503:physiopathology; D002545:Brain Ischemia / Q000209:etiology / Q000503:physiopathology; D002536:Cerebral Arteries / Q000503:physiopathology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004195:Disease Models, Animal; D005260:Female; D006439:Hemodynamics / Q000502:physiology; D008297:Male; D008833:Microcirculation / Q000502:physiology*; D010841:Pia Mater / Q000098:blood supply*; D051381:Rats; D017208:Rats, Wistar; D013345:Subarachnoid Hemorrhage / Q000150:complications / Q000503:physiopathology*; D013346:Subarachnoid Space / Q000503:physiopathology; D014661:Vasoconstriction / Q000502:physiology; D020301:Vasospasm, Intracranial / Q000209:etiology / Q000503:physiopathology*; D014699:Venules / Q000503:physiopathology