Diterpenoid production in hairy root culture of Salvia sclarea L.
Growth and diterpenoid accumulation (salvipisone, ferruginol, aethiopinone and 1-oxoaethiopinone) during the growth cycle of a Salvia sclarea hairy root culture are described. The roots transformed by Agrobacterium rhizogenes (LBA 9402) were cultured in half-strength B5 liquid medium supplemented with 30 g L(-1) sucrose under light (16 h/8 h light/dark). A culture period of 30 days was optimal for both biomass and diterpenoid production. The total content of four diterpenoids in the hairy roots [(27.3 +/- 0.6) mg g(-1) dry weight] was higher than that of roots of field-grown S. sclarea plants [(3.15 +/- 0.15) mg g(-1) dry weight]. In transformed roots, aethiopinone was the main diterpenoid, whereas the principal diterpenoid of natural roots was salvipisone.
Kuźma
Lukasz
L
Department of Biology and Pharmaceutical Botany, Medical University of Lódź, Muszyńskiego 1, 90-151 Lódź, Poland. lukikuzma@wp.pl
Bruchajzer
Elzbieta
E
Wysokińska
Halina
H
D046508:Culture Techniques; D004224:Diterpenes / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D012231:Rhizobium; D027544:Salvia / Q000378:metabolism* / Q000382:microbiology
D004224:Diterpenes