Interaction of Mycoplasma genitalium with host cells: evidence for nuclear localization.
Mycoplasma genitalium (Mg) is a mollicute that causes a range of human urogenital infections. A hallmark of these bacteria is their ability to establish chronic infections that can persist despite completion of appropriate antibiotic therapies and intact and functional immune systems. Intimate adherence and surface colonization of mycoplasmas to host cells are important pathogenic features. However, their facultative intracellular nature is poorly understood, partly due to difficulties in developing and standardizing cellular interaction model systems. Here, we characterize growth and invasion properties of two Mg strains (G37 and 1019V). Mg G37 is a high-passage laboratory strain, while Mg 1019V is a low-passage isolate recovered from the cervix. The two strains diverge partially in gene sequences for adherence-related proteins and exhibit subtle variations in their axenic growth. However, with both strains and consistent with our previous studies, a subset of adherent Mg organisms invade host cells and exhibit perinuclear targeting. Remarkably, intranuclear localization of Mg proteins is observed, which occurred as early as 30 min after infection. Mg strains deficient in adherence were markedly reduced in their ability to invade and associate with perinuclear and nuclear sites.
Ueno
Priscilla M
PM
Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Rua Professor Lineu Prestes, 1374 Sao Paulo, Brazil.
Timenetsky
Jorge
J
Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Rua Professor Lineu Prestes, 1374 Sao Paulo, Brazil.
Centonze
Victoria E
VE
Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
Wewer
Jimmy J
JJ
Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
Cagle
Marianna
M
Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
Stein
Murry A
MA
Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
Krishnan
Manickam
M
Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
Baseman
Joel B
JB
Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
D000704:Analysis of Variance; D001422:Bacterial Adhesion; D002467:Cell Nucleus / Q000382:microbiology*; D002584:Cervix Uteri / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009175:Mycoplasma Infections / Q000382:microbiology*; D045704:Mycoplasma genitalium / Q000235:genetics / Q000254:growth & development*; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial