Probing fibronectin-surface interactions: a multitechnique approach.
The development of adhesive as well as antiadhesive surfaces is essential in various biomaterial applications. In this study, we have used a multidisciplinary approach that combines biological and physicochemical methods to progress in our understanding of cell-surface interactions. Four model surfaces have been used to investigate fibronectin (Fn) adsorption and the subsequent morphology and adhesion of preosteoblasts. Such experimental conditions lead us to distinguish between anti- and proadhesive substrata. Our results indicate that Fn is not able to induce cell adhesion on antiadhesive materials. On adhesive substrata, Fn did not increase the number of adherent cells but favored their spreading. This work also examined Fn-surface interactions using ELISA immunoassays, fluorescent labeling of Fn, and force spectroscopy with Fn-modified tips. The results provided clear evidence of the advantages and limitations of each technique. All of the techniques confirmed the important adsorption of Fn on proadhesive surfaces for cells. By contrast, antiadhesive substrata for cells avoided Fn adsorption. Furthermore, ELISA experiments enabled us to verify the accessibility of cell binding sites to adsorbed Fn molecules.
Velzenberger
Elodie
E
Université de Technologie de Compiègne (UTC), BP 20529, 60205 Compiègne Cedex, France. elodie.velzenberger@utc.fr
Pezron
Isabelle
I
Legeay
Gilbert
G
Nagel
Marie-Danielle
MD
El Kirat
Karim
K
D016475:3T3 Cells; D000268:Adhesiveness; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D051379:Mice; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods; D010006:Osteoblasts / Q000166:cytology; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D005353:Fibronectins; D014867:Water