title: [Detection of infectious agents in heart valves during endocarditis using PCR technique].
abstract: Infectious endocarditis can be caused by various microorganisms. Diagnostics of local infection by microbiological methods is not always effective. For that reason we performed a study aimed for direct detection of potential infectious agents by polymerase chain reaction in patients' heart valve tissue. DNA of infectious agents was revealed in 72% of heart valve tissue samples from patients with septic endocarditis; in studied samples, along with bacterial DNA, herpesviruses' DNA was detected. Obtained results confirm the presence of infection, which allows to perform specific diagnostics of infectious complications after implantation of prosthetic cardiac valves.
authors.forename: O V
authors.initials: OV
authors.lastname: Zhirekhina
authors.forename: A B
authors.initials: AB
authors.lastname: Chukhlovin
authors.forename: K A
authors.initials: KA
authors.lastname: Sysoev
authors.forename: V V
authors.initials: VV
authors.lastname: Gritsenko
authors.forename: A A
authors.initials: AA
authors.lastname: Totolian
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D004696:Endocarditis / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D019918:Heart Valve Prosthesis Implantation / Q000009:adverse effects; D006351:Heart Valves / Q000382:microbiology* / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral