High-throughput SPR sensor for food safety.
High-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of nucleic acids identifying specific bacterial pathogens is reported. The biosensor consists of a high-performance SPR imaging sensor with polarization contrast and internal referencing (refractive index resolution 2 x 10(-7) RIU) and an array of DNA probes microspotted on the surface of the SPR sensor. It is demonstrated that short sequences of nucleic acids (20-23 bases) characteristic for bacterial pathogens such as Brucella abortus, Escherichia coli, and Staphylococcus aureus can be detected at 100 pM levels. Detection of specific DNA or RNA sequences can be performed in less than 15 min by the reported SPR sensor.
Piliarik
Marek
M
Institute of Photonics and Electronics, Chaberská 57, 182 51 Prague, Czech Republic.
Párová
Lucie
L
Homola
Jirí
J
D001420:Bacteria, Aerobic / Q000235:genetics / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
D004269:DNA, Bacterial