Evaluation of endothelial mucin layer thickness after phacoemulsification with next generation ophthalmic irrigating solution.
OBJECTIVE
To evaluate human corneal endothelial mucin layer thickness and ultrastructure after phacoemulsification and irrigation-aspiration with either next generation ophthalmic irrigating solution (NGOIS) or BSS PLUS.


METHODS
Paired human corneas were mounted in an artificial anterior chamber, exposed to 3 minutes of continuous ultrasound (US) at 80% power using the Alcon SERIES 20000 LEGACY surgical system (n = 9) or to 2 minutes of pulsed US at 50% power, 50% of the time at 20 pps using the Alcon INFINITI Vision System (n = 5), and irrigated with 250 mL of either NGOIS or BSS PLUS. A control group of paired corneas did not undergo phacoemulsification or irrigation-aspiration (n = 5). Corneas were divided and fixed for mucin staining or transmission electron microscopy. Mucin layer thickness was measured on the transmission electron microscopy prints.


RESULTS
The mucin layer thickness in the continuous phaco group was 0.77 +/- 0.02 microm (mean +/- SE) with NGOIS and 0.51 +/- 0.01 microm with BSS PLUS (t test, P < 0.001). The mucin layer thickness in the pulsed phaco group was 0.79 +/- 0.02 microm with NGOIS and 0.54 +/- 0.01 microm with BSS PLUS (P < 0.001). The mucin layer thickness in the untreated control group was 0.72 +/- 0.02 microm. The endothelial ultrastructure was normal in all corneas.


CONCLUSIONS
In this in vitro corneal model, NGOIS, due to its lower surface tension and higher viscosity, preserved endothelial mucin layer thickness better than BSS PLUS with both the INFINITI Vision System (pulsed US) and the LEGACY surgical system (continuous US).
Ghate
Deepta A
DA
Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Holley
Glenn
G
Dollinger
Harli
H
Bullock
Joseph P
JP
Markwardt
Kerry
K
Edelhauser
Henry F
HF
D000368:Aged; D003315:Cornea / Q000601:surgery* / Q000648:ultrastructure; D004728:Endothelium, Corneal / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D009077:Mucins / Q000378:metabolism* / Q000648:ultrastructure; D009883:Ophthalmic Solutions / Q000008:administration & dosage* / Q000494:pharmacology; D018918:Phacoemulsification / Q000379:methods*; D007507:Therapeutic Irrigation
D009077:Mucins; D009883:Ophthalmic Solutions