title: A novel amperometric biosensor based on NiO hollow nanospheres for biosensing glucose.
abstract: NiO hollow nanospheres were synthesized by controlled precipitation of metal ions with urea using carbon microspheres as templates, which were for the first time adopted to construct a novel amperometric glucose biosensor. Glucose oxidase was immobilized on the surface of hollow nanospheres through chitosan-assisted cross-linking technique. Due to the high specific active sites and high electrocatalytic activity of NiO hollow nanospheres, the constructed glucose biosensors exhibited a high sensitivity of 3.43 microA/mM. The low detection limit was estimated to be 47 microM (S/N=3), and the Michaelis-Menten constant was found to be 7.76 mM, indicating the high affinity of enzyme on NiO hollow nanospheres to glucose. These results show that the NiO hollow nanospheres are a promising material to construct enzyme biosensors.
authors.affiliation: Key Laboratory for Micro-Nano Optoelectronic Devices of Ministry of Education, and State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Li
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.forename: Limiao
authors.initials: L
authors.lastname: Li
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Du
authors.forename: Shoujiang
authors.initials: S
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yin
authors.forename: Taihong
authors.initials: T
authors.lastname: Wang
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D005949:Glucose Oxidase / Q000378:metabolism; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D009569:Nitric Oxide / Q000737:chemistry*; D015203:Reproducibility of Results; D014961:X-Ray Diffraction
chemical_list: D009569:Nitric Oxide; D005949:Glucose Oxidase; D005947:Glucose