title: [Determination of plasma protein binding rate of methyl protodioscin with ultrafiltration].
abstract: OBJECTIVE
  To study the plasma protein binding rate of methyl protodioscin.
  
  
  METHODS
  The ultrafiltration was employed to determine the plasma protein binding rate of methyl protodioscin. The plasma concentrations of methyl protodioscin were measured by HPLC-MS-MS.
  
  
  RESULTS
  The plasma protein binding rate of methyl protodioscin with rat plasma at the concentration of 20.0, 100 and 200 microg x mL(-1) were (94.6 +/- 0.16)%, (91.6 +/- 0.35)% and (86.10 +/- 0.60)%, respectively, while the plasma protein binding rate of methyl protodioscin with normal human plasma at the above concentrations were (82.11 +/- 5.12)%, (84.54 +/- 0.32)% and (88.52 +/- 1.02)%, respectively.
  
  
  CONCLUSIONS
  The binding rate of methyl protodioscin with plasma protein is high.
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Yao
authors.forename: Xiu-Zhen
authors.initials: XZ
authors.lastname: Cao
authors.forename: Meng
authors.initials: M
authors.lastname: Shao
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Pan
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Ye
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Yao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D011485:Protein Binding; D051381:Rats; D012503:Saponins / Q000378:metabolism*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D014462:Ultrafiltration
chemical_list: D000970:Antineoplastic Agents; D001798:Blood Proteins; D012503:Saponins; C425324:methyl protodioscin; D004144:Diosgenin