title: A protein nuclear extract from D. melanogaster larval tissues.
abstract: Preparation of protein nuclear extracts is often the first step to study in vitro biological processes occurring in the nucleus of the eukaryotic cell. Nuclear extracts have been extensively used in different model organisms to identify and study protein function in nuclei. Drosophila embryos can be collected in large quantities and have been the source of choice for the production of protein nuclear extracts. However, most of Drosophila in vivo studies on protein function are conducted in larval tissues. Here we report a new method to produce highly stable large-scale protein nuclear extracts from whole Drosophila larvae that are suited for a variety of biochemical analyses.
authors.affiliation: Istituto Telethon Dulbecco, Universita' degli Studi di Palermo, Palermo, Italy.
authors.forename: Gaspare
authors.initials: G
authors.lastname: La Rocca
authors.forename: Giosalba
authors.initials: G
authors.lastname: Burgio
authors.forename: Davide F V
authors.initials: DF
authors.lastname: Corona
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000737:chemistry; D029721:Drosophila Proteins / Q000302:isolation & purification*; D004331:Drosophila melanogaster / Q000737:chemistry*; D007814:Larva / Q000737:chemistry; D008856:Microscopy, Fluorescence; D009687:Nuclear Proteins / Q000302:isolation & purification*
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins