title: Genetic, genomic and physiological state studies on single-needle bio-electrosprayed human cells.
abstract: Bio-electrospraying, a non-contact jet-based direct cell engineering approach, was recently pioneered and demonstrated for handling a wide range of primary living cells. In those studies, post-treated cells were biologically assessed in comparison to several controls by way of flow cytometry. Although flow cytometry accurately assesses those viable populations of cells, subtle effects at a sub-cellular level could have been missed. Therefore, in the present study we demonstrate metaphase chromosome breakage studies carried out on single-needle bio-electrosprayed human T-lymphocytes, which are compared with several controls. The results indicate that post-treated T-lymphocytes do not demonstrate any increase in chromosome damage in comparison to control cells. These studies further validate bio-electrospraying as a technique with potential for clinical utility.
authors.affiliation: Cytogenetics Department, Guy's Hospital, 5th Floor Tower Wing, Great Maze Pond, London, UKSE1 9RT.
authors.forename: Richard P
authors.initials: RP
authors.lastname: Hall
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Ogilvie
authors.forename: Emma
authors.initials: E
authors.lastname: Aarons
authors.forename: Suwan N
authors.initials: SN
authors.lastname: Jayasinghe
mesh_terms: D002452:Cell Count; D002470:Cell Survival; D019457:Chromosome Breakage; D005434:Flow Cytometry; D006801:Humans; D008677:Metaphase; D013601:T-Lymphocytes / Q000648:ultrastructure*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*