Thin-layer chromatography.
TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (R(f)). Candidate compounds can be co-spotted for identification without relying on R(f) values.
Meyers
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CL
Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Meyers
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DJ
D000327:Adsorption; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D004396:Coloring Agents; D009696:Nucleic Acids / Q000302:isolation & purification*; D012997:Solvents
D004396:Coloring Agents; D009696:Nucleic Acids; D012997:Solvents