Influence of the glycosylation of human salivary proline-rich proteins on their interactions with condensed tannins.
Binding of condensed tannins to salivary proteins is supposed to be involved in their astringency. First, complexes arising from the interaction of saliva from two individuals and tannins were studied. Then interaction mixture models containing purified saliva proteins were developed. The highest polymerized tannins predominantly precipitated together with the salivary proteins. Electrophoresis of proteins in combination with thiolysis analysis of tannins indicated proline-rich protein (PRP)-polyphenol complexes in precipitated fractions and also in the soluble ones with individual differences. Individual salivas exhibiting different protein patterns were discriminated with regard to their ability to interact with tannins. From binding studies with purified classes of salivary proteins, interactions were shown to depend on the nature of the protein, in particular on their glycosylation state. For low concentrations of tannins, glycosylated PRP-tannin interactions led to complexes that remained soluble, whereas those arising from nonglycosylated PRP-tannin interactions were precipitated. This finding could indicate that under physiological conditions, complexes involving glycosylated proteins maintain part of the lubrication of the oral cavity, whereas tannin trapping leads to a lower astringency perception.
Sarni-Manchado
Pascale
P
Unité Mixte de Recherche 1083 Sciences Pour l'Oenologie, INRA, Bât 28, 2 place Viala, F-34060 Montpellier, France. sarni@supagro.inra.fr
Canals-Bosch
Joan-Miguel
JM
Mazerolles
Gérard
G
Cheynier
Véronique
V
D005260:Female; D006031:Glycosylation; D006801:Humans; D008970:Molecular Weight; D044945:Proanthocyanidins / Q000737:chemistry / Q000378:metabolism*; D011392:Proline / Q000378:metabolism*; D011485:Protein Binding; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D027843:Vitis / Q000737:chemistry
D044945:Proanthocyanidins; D012471:Salivary Proteins and Peptides; D011392:Proline