title: Purification, physicochemical properties, thermal analysis and antinociceptive effect of atranorin extracted from Cladina kalbii.
abstract: Lichens and their secondary metabolites have attracted the interest of many researchers. Some species have been shown to contain substances with remarkable biologic activity, as antimicrobial, mainly against Gram positive bacteria; antineoplasic acting on solid and ascetic tumors, or in culture cells; antiviral; hypotensive; and spasmolytic effects. The aim of this study was to isolate and characterize atranorin, one of the major constituents which presents in Cladina kalbii (DES ABB.) AHTI., and analyze its antinociceptive effect. The antinociceptive activity was verified in acetic acid-induced writhing test and formalin test with mice. In this work it was observed that atranorin was effective in significant reducing (p<0.05) abdominal writhing at doses of 200 and 400 mg/kg (p.o.) by 52.6 and 61.3%, respectively, when compared to control group (vehicle). The formalin test showed in 200 and 400 mg/kg (p.o.) that atranorin injection was able to inhibit the inflammatory processes (second phase) dose dependently.
authors.affiliation: Laboratório de Ensaios Farmacêuticos e Toxicidade, Universidade Federal de Sergipe (LeFT/UFS), São Cristóvão-SE, CEP 49000-000, Brazil.
authors.forename: Marcélia Garcez Dória
authors.initials: MG
authors.lastname: Melo
authors.forename: Adriano Antunes Souza
authors.initials: AA
authors.lastname: Araújo
authors.forename: Carla Paula Leite
authors.initials: CP
authors.lastname: Rocha
authors.forename: Emyle Mayra Santana Alves
authors.initials: EM
authors.lastname: Almeida
authors.forename: Rosana de Souza
authors.initials: Rde S
authors.lastname: Siqueira
authors.forename: Leonardo Rigoldi
authors.initials: LR
authors.lastname: Bonjardim
authors.forename: Lucindo José
authors.initials: LJ
authors.lastname: Quintans
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D001241:Aspirin / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004055:Differential Thermal Analysis; D005557:Formaldehyde; D062385:Hydroxybenzoates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008011:Lichens / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D010147:Pain Measurement / Q000187:drug effects; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
chemical_list: D000700:Analgesics; D062385:Hydroxybenzoates; D005557:Formaldehyde; C026304:atranorin; D019342:Acetic Acid; D001241:Aspirin