Molecular characterization of VP6-encoding gene of group A human rotavirus samples from central west region of Brazil.
Group A rotaviruses are the main cause of acute gastroenteritis in children worldwide. The intermediate capsid protein VP6 encoded by segment 6 of the dsRNA genome is the major structural component of the virus and it is highly antigenic and immunogenic. VP6 is responsible for group and subgroup (SG) specificities, allowing classification of group A rotavirus into SG I, SG II, SG I + II, and SG non-I-non-II. VP6-encoding gene of 154 group A human rotavirus samples of different G and P genotypes recovered from children in three cities of Central West region of Brazil was amplified by reverse transcription-polymerase chain reaction followed by sequencing and phylogenetic analysis. Two distinct genetic groups could be recognized: VP6 genogroups I and II. Sequences analysis also revealed that all samples identified as VP6 genogroup I were associated with NSP4 genotype A, whereas samples identified as VP6 genogroup II were associated with NSP4 genotype B. This is the first study in Central West region regarding genetic variability of the VP6 gene. Further molecular surveillance of rotavirus strains is needed to understand better the occurrence of VP6 gene diversity in Brazil and the significance of VP6 for the control and prevention of rotavirus gastroenteritis.
Tavares
Talissa de Moraes
Tde M
Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Brazil.
de Brito
Wilia Marta Elsner Diederichsen
WM
Fiaccadori
Fabíola Souza
FS
Parente
Juliana Alves
JA
da Costa
Paulo Sérgio Sucasas
PS
Giugliano
Loreny Gimenes
LG
Andreasi
Márcia Sueli Assis
MS
Soares
Célia Maria Almeida
CM
Cardoso
Divina das Dôres de Paula
Dd
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics*; D001938:Brazil / Q000453:epidemiology; D036022:Capsid Proteins / Q000235:genetics*; D002675:Child, Preschool; D016000:Cluster Analysis; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C061332:VP6 protein, Rotavirus