title: Growth enhancement of effective microorganisms for bioremediation of crude oil contaminated waters.
abstract: The bioremediation of polluted groundwater, wastewater aeration pond and biopond sites was investigated using bacteria isolated from these sites located at the oil refinery Terengganu Malaysia. Out of 62 isolates, only 16 isolates from groundwater (8) and wastewater aeration pond (3) and biopond (5) were chosen based on growth medium containing 1% (v/v) Tapis crude oil. Only four isolates; Acinetobacter faecalis, Staphylococcus sp., Pseudomonas putida and Neisseria elongata showed percentage biodegradation of crude oil more than 50% after 5 days using Mineral Salts Medium (MSM). The effect of physical parameters (temperature, pH and agitation) on growth by all four strains showed a maximum growth in MSM medium with 1% Tapis crude oil at 37 degrees C with pH 7 and agitation of 130 rpm.
authors.affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM, 43600 Bangi, Selangor, Malaysia.
authors.forename: Abdualdaim Mohammed
authors.initials: AM
authors.lastname: Mukred
authors.forename: Aidil
authors.initials: A
authors.lastname: Abd-Hamid
authors.forename: Ainon
authors.initials: A
authors.lastname: Hamzah
authors.forename: Wan Mohtar Wan
authors.initials: WM
authors.lastname: Yusoff
mesh_terms: D001673:Biodegradation, Environmental; D006863:Hydrogen-Ion Concentration; D010578:Petroleum / Q000378:metabolism* / Q000382:microbiology*; D013696:Temperature; D014871:Water Microbiology; D014877:Water Pollution, Chemical / Q000032:analysis*
chemical_list: D010578:Petroleum