title: Opisthorchis viverrini: detection by polymerase chain reaction (PCR) in human stool samples.
abstract: A polymerase chain reaction (PCR) assay was evaluated for detection of Opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in Khon Kaen province of Thailand. A total of 75 fecal specimens were analyzed by PCR following DNA extraction. All the microscopically positive samples were positive by PCR, while 23 of 30 (76.6%) microscopically negative samples were also PCR positive. The sensitivity of the assay was 5 eggs/g of stool. This method is potentially useful in the diagnosis of human opisthorchiasis in endemic areas for treatment and in epidemiological investigations.
authors.affiliation: Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Kankanady Post, Yekkur, Mangalore 575 002, India.
authors.forename: Kanasinakatte R
authors.initials: KR
authors.lastname: Umesha
authors.forename: Sanath
authors.initials: S
authors.lastname: Kumar
authors.forename: Ammini
authors.initials: A
authors.lastname: Parvathi
authors.forename: Kunyarat
authors.initials: K
authors.lastname: Duenngai
authors.forename: Paiboon
authors.initials: P
authors.lastname: Sithithaworn
authors.forename: Indrani
authors.initials: I
authors.lastname: Karunasagar
authors.forename: Iddya
authors.initials: I
authors.lastname: Karunasagar
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005260:Female; D006801:Humans; D008297:Male; D009889:Opisthorchiasis / Q000175:diagnosis* / Q000469:parasitology; D009891:Opisthorchis / Q000235:genetics / Q000302:isolation & purification*; D010063:Ovum; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D018509:DNA, Helminth