Engineering of avermectin biosynthetic genes to improve production of ivermectin in Streptomyces avermitilis.
Two new recombinants of avermectin polyketide synthases were constructed by domain and module swapping in Streptomyces avermitilis 73-12. However, only the strain, S. avermitilis OI-31, formed by domain substitution could produce ivermectin. Analysis of the ivermectin synthesized gene cluster showed that decreased amount of aveC transcripts was one of the factors causing low yield of ivermectin. Overexpression of aveC could improve ivermectin yield.
Li
Meng
M
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, Beijing 100193, PR China.
Chen
Zhi
Z
Lin
Xiuping
X
Zhang
Xuan
X
Song
Yuan
Y
Wen
Ying
Y
Li
Jilun
J
D000595:Amino Acid Sequence; D000977:Antiparasitic Agents / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D020650:Combinatorial Chemistry Techniques; D015195:Drug Design; D005818:Genetic Engineering / Q000379:methods*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000737:chemistry*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D005810:Multigene Family; D009711:Nucleotides / Q000737:chemistry; D013302:Streptomyces / Q000378:metabolism*
D000977:Antiparasitic Agents; D009711:Nucleotides; D007559:Ivermectin; C019264:avermectin