A new strain of Streptomyces avermitilis produces high yield of oligomycin A with potent anti-tumor activity on human cancer cell lines in vitro.
A new actinomycete strain, isolated from soil in China, strongly inhibited in vitro proliferation of human hepatoma, chronic myelogenous leukemia, and colonic carcinoma cell lines. The strain, designated L033, was identified as a strain of Streptomyces avermitilis based on cultural property, morphology, carbon source utilization, 16s rRNA gene analysis, and DNA-DNA relatedness studies. The anticancer component from L033 was purified to homogeneity by preparative positive-phase high-performance liquid chromatography and crystallization. Nuclear magnetic resonance and mass spectrometric analysis showed that this compound had the same structure as oligomycin A. Different with other reported naturally occurring strains of S. avermitilis, L033 produced high quantity of oligomycin A (maximal 1,461 microg/ml). Therefore, L033 was considered of great potential as an industrial oligomycin-A-producing strain.
Lin
Xiuping
X
State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
Wen
Ying
Y
Li
Meng
M
Chen
Zhi
Z
Guo
Jia
J
Song
Yuan
Y
Li
Jilun
J
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D050260:Carbohydrate Metabolism; D045744:Cell Line, Tumor; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009840:Oligomycins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D000970:Antineoplastic Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009840:Oligomycins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C031004:oligomycin A