Expression and localisation of claudin-1, -2, -3, -4, -5, -7 and -10 proteins in the normal canine mammary gland.
The recently identified claudins are dominant components of tight junctions, responsible for cell adhesion, polarity and paracellular permeability. Certain claudins have been shown to have relevance in tumour development. The aim of the present study was to analyse the expression of claudin-1, -2, -3, -4, -5, -7 and -10 in normal canine mammary glands. Samples from the inguinal mammary regions of 20 non-castrated, 1-13 years old female dogs were studied. Immunohistochemical analysis was performed on conventional specimens and tissue microarrays. The results of the immunohistochemical reactions detecting claudins in tissue sections were photodocumented. The immunoreactivity of claudins was quantitatively analysed on digital images using Leica QWin morphometry software. Intense membranous immunolabelling was found for claudin-1, -3 and -7, intense membranous with non-granular cytoplasmic immunolabelling for claudin-2, moderate membranous immunolabelling for claudin-4 and -5, and weak membranous immunolabelling for claudin-10. The occurrence of tight junctions was confirmed by ultrathin section electron microscopy. The available data suggested that claudins might be proteins preserved throughout the evolution of mammals. The results of our study support the concept that they are indeed preserved, since the same type of claudins, in identical distribution, could be detected in our canine mammary tissue samples as could be found in human mammary tissue.
Jakab
Csaba
C
Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, Szent István University, H-1078 Budapest, István u. 2, Hungary. Jakab.Csaba@aotk.szie.hu
Halász
Judit
J
Szász
Attila Marcell
AM
Batmunkh
Enkhjargal
E
Kiss
András
A
Schaff
Zsuzsa
Z
Rusvai
Miklós
M
Gálfi
Péter
P
Kulka
Janina
J
D000818:Animals; D004285:Dogs; D005260:Female; D020869:Gene Expression Profiling; D007150:Immunohistochemistry / Q000662:veterinary; D008321:Mammary Glands, Animal / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008854:Microscopy, Electron; D019108:Tight Junctions / Q000502:physiology* / Q000648:ultrastructure; D046888:Tissue Array Analysis / Q000662:veterinary
D008565:Membrane Proteins