title: Quantitative fluorescent polymerase chain reaction to detect chromosomal anomalies in spontaneous abortion.
abstract: OBJECTIVE
  To evaluate the value of short tandem repeats (microsatellites) in the study of numerical chromosomal anomalies in spontaneous abortion.
  
  
  METHODS
  Multiplex quantitative fluorescent polymerase chain reaction (QF-PCR) was carried out on 61 spontaneous abortion samples and 48 controls using microsatellite markers from 8 chromosomes where aneuploids are commonly found.
  
  
  RESULTS
  Of the 61 samples, 65.6% were successfully karyotyped, and the call rate of the QF-PCR was 98.3%. The correspondence between PCR and karyotyping was 95%. The success rate of karyotyping in the inevitable abortion group was 79.6%, higher than for the missed abortion group (8.3%), P<0.001. The call rate of QF-PCR showed no difference between these 2 groups (100% vs 91.7%, P=0.197).
  
  
  CONCLUSIONS
  Microsatellite-based QF-PCR is a helpful and reliable tool to diagnose numerical chromosomal anomalies in spontaneous abortion. It also provides a diagnosis for necrotic tissue.
authors.affiliation: Shanghai First Maternity and Infant Hospital of Tongji University, Shanghai, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zou
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Xing W
authors.initials: XW
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Guang
authors.initials: G
authors.lastname: He
authors.forename: Tony
authors.initials: T
authors.lastname: Duan
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics*; D000328:Adult; D000782:Aneuploidy; D016022:Case-Control Studies; D025063:Chromosome Disorders / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D005453:Fluorescence; D005819:Genetic Markers; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D007621:Karyotyping / Q000379:methods; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D011247:Pregnancy; D015203:Reproducibility of Results
chemical_list: D005819:Genetic Markers