Antioxidant activity of Sanionia uncinata, a polar moss species from King George Island, Antarctica.
Antioxidant agents counter reactive oxygen species (ROS) and can be used in cosmetic and medicinal applications. The goal of this study was to evaluate the antioxidant activity of an Antarctic moss species from King George Island (Antarctica), tentatively designated as KSJ-M5. On the basis of morphological characteristics, KSJ-M5 was identified as Sanionia uncinata (Hedw.) Loeske (Amblystegiaceae). The identification was confirmed by comparing the partial sequence of the ITS (internal transcribed spacer) region with that in GenBank. The antioxidant activity of an ethanol extract of KSJ-M5 was evaluated by analyzing its reducing power, superoxide scavenging activity, ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)] cation scavenging activity, and DPPH (1,1-diphenyl-2-picrylhydrazyl) free-radical scavenging activity. The reducing power of 1 mg of KSJ-M5 extract was equivalent to 31.9 +/- 0.9 microg (Mean +/- SD, n = 3) of the commercial standard, BHT (butylated hydroxytoluene). IC(50) values of the KSJ-M5 extract for DPPH free-radical scavenging activity, superoxide scavenging activity, and ABTS cation scavenging activity were found as 356 +/- 26.8 microg/mL, 466.2 +/- 43.4 microg/mL, and 181.3 +/- 12.2 microg/mL, respectively. The total phenolic content in 1 mg of KSJM5 extract was equivalent to 12.7 +/- 2.7 microg of pyrocatechol. These results clearly showed that KSJ-M5 could be an important source of natural antioxidant agents for improved medicinal and cosmetic applications.
Bhattarai
Hari Datta
HD
Polar BioCenter, Korea Polar Research Institute, KOPRI Songdo Technopark, Yeonsu-gu, Incheon 406-840, South Korea.
Paudel
Babita
B
Lee
Hyoung Seok
HS
Lee
Yoo Kyung
YK
Yim
Joung Han
JH
D000864:Antarctic Regions; D052160:Benzothiazoles / Q000378:metabolism; D001713:Biphenyl Compounds; D019068:Bryopsida / Q000737:chemistry*; D002084:Butylated Hydroxytoluene / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D010636:Phenols / Q000032:analysis; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; D013481:Superoxides; D002084:Butylated Hydroxytoluene; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl