Dexamethasone increases angiopoietin-1 and quiescent hematopoietic stem cells: a novel mechanism of dexamethasone-induced hematoprotection.
Angiopoietin-1 (Ang-1) is known to have hematoprotective effects by increasing the quiescence of hematopoietic stem cells. However, it remains to be determined if the upregulation of Ang-1 and the subsequent increase in the quiescence of hematopoietic stem cells are also involved in the dexamethasone (Dex)-mediated bone marrow protection. Here Western blotting and flow cytometric analyses demonstrate that Dex increases the levels of Ang-1 in mouse bone marrow and the quiescence of hematopoietic stem cells. Our data for the first time suggest that the increased quiescence of hematopoietic stem cells provides a novel mechanism of Dex-induced hematoprotection.
Kim
Hyongbum
H
Department of Biomedical Sciences, National Institute of Health, Seoul, South Korea.
Choi
Ji-Young
JY
Lee
Jung Min
JM
Park
Yoon Shin
YS
Suh
Hwal
H
Song
Hae-Ryong
HR
Jo
Sangmee Ahn
SA
Jo
Inho
I
D042683:Angiopoietin-1 / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D019610:Cytoprotection; D003907:Dexamethasone / Q000494:pharmacology*; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D023461:Myeloid Progenitor Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020871:RNA Stability / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis / Q000235:genetics; D020218:Response Elements
D042683:Angiopoietin-1; D005938:Glucocorticoids; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone