Culture of mouse neural stem cell precursors.
Primary neural stem cell cultures are useful for studying the mechanisms underlying central nervous system development. Stem cell research will increase our understanding of the nervous system and may allow us to develop treatments for currently incurable brain diseases and injuries. In addition, stem cells should be used for stem cell research aimed at the detailed study of mechanisms of neural differentiation and transdifferentiation and the genetic and environmental signals that direct the specialization of the cells into particular cell types. This video demonstrates a technique used to disaggregate cells from the embryonic day 12.5 mouse dorsal forebrain. The dissection procedure includes harvesting E12.5 mouse embryos from the uterus, removing the "skin" with fine dissecting forceps and finally isolating pieces of cerebral cortex. Following the dissection, the tissue is digested and mechanically dissociated. The resuspended dissociated cells are then cultured in "stem cell" media that favors growth of neural stem cells.
Currle
D Spencer
DS
Department of Developmental and Cell Biology, University of California, Irvine, CA, USA. dcurrle@uci.edu
Hu
Jia Sheng
JS
Kolski-Andreaco
Aaron
A
Monuki
Edwin S
ES
D000818:Animals; D018929:Cell Culture Techniques; D004210:Dissection / Q000379:methods; D053595:Embryonic Stem Cells / Q000166:cytology*; D051379:Mice; D009474:Neurons / Q000166:cytology*; D016548:Prosencephalon / Q000196:embryology / Q000601:surgery; D020858:Tissue and Organ Harvesting / Q000379:methods