The beneficial effect of coenzyme Q10 and lipoic acid on obstructive bladder dysfunction in the rabbit.
OBJECTIVE
Recent evidence indicates that ischemia and reperfusion are major etiological factors in the bladder dysfunction that occurs after partial bladder outlet obstruction. Coenzyme Q10 and alpha-lipoic acid are found naturally in mitochondria and act as potent antioxidants. We investigated the beneficial effects of coenzyme Q10 plus alpha-lipoic acid in a rabbit model of bladder outlet obstruction.


METHODS
Twenty male rabbits were divided into 5 groups. Group 1 served as control and group 2 received three weeks of coenzyme Q10 plus alpha-lipoic acid supplementation. Rabbits in group 3 underwent surgical partial bladder outlet obstruction for duration of four weeks and groups 4 and 5 were obstructed for seven weeks. In group 5, coenzyme Q10 plus alpha-lipoic acid supplementation was given following 4 weeks obstruction and continued till the end of the seven weeks. The contractile responses to various agents were determined. The protein nitration and carbonylation levels were studied by immunoblotting. Nerve function was determined by choline acetyltransferase activity and nerve density.


RESULTS
The contractile responses to different forms of stimulations, including field stimulation, ATP, carbachol and KCl all showed decreases following 4 and 7 weeks obstruction. Treatment with coenzyme Q10 plus alpha-lipoic acid significantly restored contractile responses to all forms of stimulation. Treatment also had mitochondrial and neuronal effects and reduced protein nitration and carbonylation. Histologically there was less detrusor muscle hypertrophy.


CONCLUSIONS
The current study clearly demonstrates that coenzyme Q10 and alpha-lipoic acid supplementation can improve bladder function after outlet obstruction.
Juan
Yung-Shun
YS
Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China.
Levin
Robert M
RM
Chuang
Shu Mien
SM
Hydery
Tasmina
T
Li
Suning
S
Kogan
Barry
B
Schuler
Catherine
C
Huang
Chun-Hsiung
CH
Mannikarottu
Anita
A
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D007150:Immunohistochemistry; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D011336:Probability; D011817:Rabbits; D011897:Random Allocation; D012680:Sensitivity and Specificity; D008063:Thioctic Acid / Q000494:pharmacology*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D001748:Urinary Bladder Neck Obstruction / Q000188:drug therapy* / Q000473:pathology*
D014451:Ubiquinone; D008063:Thioctic Acid; C024989:coenzyme Q10