Design, synthesis and evaluation of D-galactose-beta-cyclodextrin conjugates as drug-carrying molecules.
Several kinds of D-galactose-beta-cyclodextrin conjugates having a phenyl group in the spacers between the D-galactose and beta-cyclodextrin were designed and synthesized as drug-carrying molecules. Their evaluation as drug-carrying molecules was done by measuring the molecular interactions with the anticancer agent, doxorubicin, and with the d-galactose-binding peanut lectin using an SPR optical biosensor. The SPR analyses showed that these conjugates had remarkably high inclusion associations of 10(5) approximately 10(7)M(-1) levels for the immobilized doxorubicin. Their association constants for immobilized peanut lectin were at the level of 10(4) approximately 10(5)M(-1), as we expected. These conjugates will be useful drug-carrying models which can site-specifically carry doxorubicin to the cells containing D-galactose-binding lectin.
Oda
Yoshiki
Y
The Noguchi Institute, 1-8-1 Kaga, Itabashi-ku, Tokyo 173-0003, Japan.
Yanagisawa
Hironari
H
Maruyama
Machiko
M
Hattori
Kenjiro
K
Yamanoi
Takashi
T
D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods; D004317:Doxorubicin / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000138:chemical synthesis / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods*; D015195:Drug Design; D005690:Galactose / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000494:pharmacology*; D019887:Peanut Agglutinin / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D020349:Surface Plasmon Resonance / Q000379:methods; D047392:beta-Cyclodextrins / Q000138:chemical synthesis / Q000494:pharmacology*
D000970:Antineoplastic Agents; D004337:Drug Carriers; D019887:Peanut Agglutinin; D047392:beta-Cyclodextrins; D004317:Doxorubicin; D005690:Galactose