Characterization of two Campylobacter jejuni strains for use in volunteer experimental-infection studies.
The development of vaccines against Campylobacter jejuni would be facilitated by the ability to perform phase II challenge studies. However, molecular mimicry of the lipooligosaccharide (LOS) of most C. jejuni strains with human gangliosides presents safety concerns about the development of Guillain-Barré syndrome. Clinical isolates of C. jejuni that appeared to lack genes for the synthesis of ganglioside mimics were identified by DNA probe analyses. Two clinical isolates from Southeast Asia (strains BH-01-0142 and CG8421) were determined to express the LOS type containing N-acetyl quinovosamine. No ganglioside structures were observed to be present in the LOSs of these strains, and pyrosequence analyses of the genomes of both strains confirmed the absence of genes involved in ganglioside mimicry. The capsule polysaccharide (CPS) of BH-01-0142 was determined to be composed of galactose (Gal), 6-deoxy-ido-heptose, and, in smaller amounts, D-glycero-D-ido-heptose, and the CPS of CG8421 was observed to contain Gal, 6-deoxy-altro-heptose, N-acetyl-glucosamine, and minor amounts of 6-deoxy-3-O-Me-altro-heptose. Both CPSs were shown to carry O-methyl-phosphoramidate. The two genomes contained strain-specific zones, some of which could be traced to a plasmid origin, and both contained a large chromosomal insertion related to the CJEI3 element of C. jejuni RM1221. The genomes of both strains shared a high degree of similarity to each other and, with the exception of the capsule locus of CG8421, to the type strain of the HS3 serotype, TGH9011.
Poly
Frédéric
F
Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
Read
Timothy D
TD
Chen
Yu-Han
YH
Monteiro
Mario A
MA
Serichantalergs
Oralak
O
Pootong
Piyarat
P
Bodhidatta
Ladaporn
L
Mason
Carl J
CJ
Rockabrand
David
D
Baqar
Shahida
S
Porter
Chad K
CK
Tribble
David
D
Darsley
Michael
M
Guerry
Patricia
P
D001483:Base Sequence; D002169:Campylobacter Infections / Q000235:genetics / Q000276:immunology / Q000517:prevention & control; D016123:Campylobacter jejuni / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology; D008401:Gas Chromatography-Mass Spectrometry; D006805:Human Experimentation; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*
D005732:Gangliosides; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; C023023:lipid-linked oligosaccharides