title: A novel and rapid method for determining integration frequency catalyzed by integron integrase intI1.
abstract: We developed a faster and more convenient method to determine integration frequency mediated by integron integrase intI1. This method based on real-time fluorescent quantitative PCR. By using this method, we revealed that the integration frequency of aadA2 gene cassette was 1.87x10(-4) when integrase intI1 was present, and the background frequency was less than 5.23x10(-8) without integrase intI1.
authors.affiliation: Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Yang
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Jiang
authors.forename: Quhao
authors.initials: Q
authors.lastname: Wei
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005453:Fluorescence; D018628:Gene Dosage; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D032023:Integrons; D016133:Polymerase Chain Reaction / Q000379:methods*; D011995:Recombination, Genetic
chemical_list: D019426:Integrases; C115006:integron integrase IntI1