title: Immunohistoblot analysis on whole human hemispheres from normal and Alzheimer diseased brains.
abstract: We demonstrate the feasibility and usefulness of the histoblot immunostaining of cryosections of whole hemispheres of healthy and Alzheimer diseased (AD) human brains by localizing a neuron-specific marker, the anti-neuronal nuclei (NeuN) antigen. As expected, cortical NeuN-immunopositive regions were generally thinner and lighter in the AD brains than in the controls. The advantages of using whole hemisphere histoblots: (1) they provide a low-resolution overview/outline of the antigen distribution in a large surface area, (2) large, thick, and/or unfixed tissue sections from post-mortem samples (perhaps of inferior tissue quality) can be compared, and (3) subsequent immunohistochemistry can be performed on the tissue sections used for the histoblots.
authors.affiliation: Department of Cell Biology and Molecular Medicine, University of Szeged, Szeged H-6720, Hungary.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Beliczai
authors.forename: Szilvia
authors.initials: S
authors.lastname: Varszegi
authors.forename: Balazs
authors.initials: B
authors.lastname: Gulyas
authors.forename: Christer
authors.initials: C
authors.lastname: Halldin
authors.forename: Peter
authors.initials: P
authors.lastname: Kasa
authors.forename: Karoly
authors.initials: K
authors.lastname: Gulya
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D034961:Antigens, Nuclear / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002540:Cerebral Cortex / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D003101:Collodion; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008567:Membranes, Artificial; D008867:Microtomy; D008875:Middle Aged; D009410:Nerve Degeneration / Q000378:metabolism / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D034961:Antigens, Nuclear; D015415:Biomarkers; D008567:Membranes, Artificial; D009419:Nerve Tissue Proteins; C506808:neuronal nuclear antigen NeuN, human; D003101:Collodion