Effect of 2,4-dichlorophenoxyacetic acid on growth, protein and chlorophyll-a content of Chlorella vulgaris and Spirulina platensis cells.
In this study, effect of different 2,4 -dichlorophenoxyacetic acid (2,4-D) concentrations (0.0, 9.10(-5), 9.10(-4), 9.10(-3) and 9.10(-2) mM) on growth rate, content of protein and chlorophyll-a in Chlorella vulgaris and Spirulina platensis cells was investigated. The most stimulatory effect on growth rate, protein and pigment ratio of C. vulgaris and S. platensis was observed at 9.10(-4) mM concentrations of 2,4-D. The results show that low concentrations of 2,4-D have hormonal effect due to being a synthetic auxin. Cell number protein and pigment rates were inhibited at 9.10(-2) mM concentration in C. vulgaris. Such parameters were inhibited in S. platensis, both at 9.10(-3) and 9.10(-2) mM 2,4-D concentrations. This is due to herbicidal effect of high concentrations of 2,4-D. S. platensis was found to be more sensitive than S. vulgaris to 2,4-D applications. The use of algae as bio-indicators in herbicide contaminated fresh water habitats, was discussed.
Saygideger
Saadet Demirors
SD
Department of Biology, Faculty of Arts and Sciences, University of Gaziantep, Gaziantep, Turkey. saygideger@gantep.edu.tr
Okkay
Ozlem
O
D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism / Q000633:toxicity*; D048408:Chlorella vulgaris / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D004305:Dose-Response Relationship, Drug; D005618:Fresh Water; D006540:Herbicides / Q000378:metabolism / Q000633:toxicity*; D007700:Kinetics; D009687:Nuclear Proteins / Q000378:metabolism*; D010860:Pigments, Biological / Q000037:antagonists & inhibitors / Q000378:metabolism; D053545:Spirulina / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D006540:Herbicides; D009687:Nuclear Proteins; D010860:Pigments, Biological; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D015084:2,4-Dichlorophenoxyacetic Acid; D000077194:Chlorophyll A