Enantioselective analysis of primaquine and its impurity quinocide by capillary electrophoresis.
A capillary electrophoretic (CE) method for the baseline separation of the enantiomers of primaquine diphosphate (PQ) and quinocide (QC) (a major contaminant) in pharmaceutical formulations is proposed. Both components were separated under the following conditions: 50 mm tris phosphate buffer (pH 3.0) containing 15 mm hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD) as background electrolyte; applied voltage, 16 kV; capillary temperature, 25 degrees C; detection wavelength, 254 nm; hydrostatic injection, 10 s. The separations were conducted using a 35 cm length and 50 microm i.d. uncoated fused silica capillary column. Under the optimized conditions, the components were successfully separated in about 5 min. Intraday precision of migration time and corrected peak areas when expressed as relative standard deviation ranged from 0.17 to 0.45 and 2.60 to 3.94%, respectively, while the interday precision ranged from 2.59 to 4.20 and 3.15 to 4.21%, respectively. After the validation exercise, the proposed method was applied for the determination of QC impurity in PQ formulations.
Elbashir
Abdalla A
AA
Universiti Sains Malaysia, Penang.
Saad
Bahruddin
B
Ali
Abdussalam Salhin Mohamed
AS
Saleh
Muhammad Idiris
MI
Aboul-Enein
Hassan Y
HY
D000634:Aminoquinolines / Q000737:chemistry*; D000962:Antimalarials / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry; D004340:Drug Contamination; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008956:Models, Chemical; D011319:Primaquine / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013607:Tablets / Q000737:chemistry; D013696:Temperature
D000634:Aminoquinolines; D000962:Antimalarials; D003505:Cyclodextrins; D013607:Tablets; C010775:quinocide; D011319:Primaquine