title: Heat shock effect upon dengue virus replication into U937 cells.
abstract: The molecules involved in dengue virus entry into human cells are currently unknown. We have previously shown that two surface heat shock proteins (Hsps), Hsp90 and Hsp70 are part of a receptor complex in monocytic cells. In the present report, the effect of heat shock (HS) on dengue virus infection is analyzed. We have documented a more than twofold increase in dengue virus infectivity after HS treatment in monocytic cells U937; this effect correlates mainly with an increase in viral entry due to a major presence of both Hsps on the surface of monocytic cells, particularly in membrane microdomains. Interestingly, since heat shock treatment at 6h post-infection also increased viral yields, it is likely that HS also modulates positively dengue virus replication.
authors.affiliation: Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del IPN, Av. I.P.N. 2508, Col. San Pedro Zacatenco, México D.F. C.P. 07360, Mexico.
authors.forename: Salvador
authors.initials: S
authors.lastname: Chavez-Salinas
authors.forename: Ivonne
authors.initials: I
authors.lastname: Ceballos-Olvera
authors.forename: Jorge
authors.initials: J
authors.lastname: Reyes-Del Valle
authors.forename: Fernando
authors.initials: F
authors.lastname: Medina
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Del Angel
mesh_terms: D002462:Cell Membrane / Q000378:metabolism / Q000821:virology; D003715:Dengue / Q000378:metabolism / Q000503:physiopathology* / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response; D006801:Humans; D009000:Monocytes / Q000502:physiology* / Q000821:virology; D020298:U937 Cells; D053586:Virus Internalization; D014779:Virus Replication
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins