Photoresponsive surfaces with two independent wavelength-selective functional levels.
Two photoremovable protecting groups, namely, nitroveratryloxycarbonyl (NVo) and diethylamino-coumarin-4-yl (DEACM), have been tested for wavelength-selective, independent removal. The chromophores were attached to the amine group of aminopropyltriethoxysilane and used for the modification of silica surfaces. A photolytic experiment on the photosensitive layers allowed us to identify the irradiation conditions for the selective cleavage of the chromophores. UV measurements revealed that the photolabile DEACM group can be cleaved off with UV light at 412 nm without damaging the NVo group. The NVo group could then be removed at 365 nm. Masked irradiation of substrates modified with a 1:1 molar mixture of both silanes allowed the generation of bifunctional patterns after the selective cleavage of DEACM and NVo in a sequential irradiation process. The deprotection reaction was confirmed by coupling two different fluorescent dyes to the liberated amine groups. The expected two-color pattern could be observed by fluorescence microscopy.
Stegmaier
Petra
P
Max-Planck-Institut fur Metallforschung, Heisenbergstrasse 3, 70569 Stuttgart, Germany.
Alonso
José María
JM
Campo
Aránzazu Del
AD
D002627:Chemistry, Physical / Q000379:methods; D004867:Equipment Design; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000494:pharmacology; D008027:Light; D008856:Microscopy, Fluorescence / Q000379:methods; D008956:Models, Chemical; D010777:Photochemistry / Q000379:methods*; D012821:Silanes / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013499:Surface Properties; D014466:Ultraviolet Rays
D005456:Fluorescent Dyes; D012821:Silanes