Calpain inhibitory flavonoids isolated from Orostachys japonicus.
The n-butanol (n-BuOH) fraction of Orostachys japonicus A. Berger (Crassulaceae) significantly inhibited calpain activity. Through the activity-guided isolation from the n-BuOH fraction, herbacetin 8-O-alpha-D-ribopyranoside (1), kaempferol (2), quercetin (3), afzelin (4), astragalin (5), isoquercetin (6) and quercitrin (7) were obtained. Their structures were determined by spectroscopic techniques. Among them, compound 3 and 5 had significant calpain inhibitory activities.
Je Ma
Choong
C
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, 151-742, Republic of Korea.
Jung
Won Joo
WJ
Lee
Ki Yong
KY
Kim
Young Choong
YC
Sung
Sang Hyun
SH
D020001:1-Butanol / Q000737:chemistry; D002154:Calpain / Q000037:antagonists & inhibitors* / Q000378:metabolism; D029627:Crassulaceae / Q000737:chemistry*; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011794:Quercetin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D015853:Cysteine Proteinase Inhibitors; D005419:Flavonoids; D044949:Kaempferols; D018696:Neuroprotective Agents; D010936:Plant Extracts; D020001:1-Butanol; D011794:Quercetin; C001579:astragalin; D002154:Calpain