[Study of Scutellaria baicalensis and Baicalin against antimicrobial susceptibility of Helicobacter pylori strains in vitro].
OBJECTIVE
To compare the antimicrobial activities of Scutellaria baicalensis and Baicalin against Helicobacter pylori (H. pylori) in vitro.


METHODS
The crude alcohol extraction of Scutellaria baicalensis was obtained by successive extractions with ethanol. Baicalin was extracted by using organic extraction methods and the purity was analyzed by high-performance liquid chromatography (HPLC). The crude alcohol extraction of Scutellaria baicalensis and Baicalin were used in broth dilution assays to test for antibacterial properties.


RESULTS
The content of Baicalin was 5.01 g from 100 g Scutellaria baicalensis. The purity of Baicalin was 96.8%. The crude alcohol extraction of Scutellaria baicalensis and Baicalin were tested for their ability to inhibit H. pylori in vitro by using broth dilution assays. The MIC50 and MIC90 of Baicalin against ten strains of H. pylori were 1.04 and 1.30 mg/ml respectively. The MIC50 and MIC90 of the crude product against ten strains of H. pylori were 2.60 and 3.26 mg/ml respectively.


CONCLUSIONS
The Baicalin and Scutellaria baicalensis are bactericidal against H. pylori. The antimicrobial activity of Baicalin is greater than that of Scutellaria baicalensis.
Wu
Jing
J
Medical College, Anhui University of Science and Technology, Huainan 232001, China.
Hu
Dong
D
Wang
Ke-Xia
KX
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D024881:Drug Resistance, Bacterial; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods
D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C038044:baicalin; D000431:Ethanol