Role of Ebola virus VP30 in transcription reinitiation.
VP30 is a phosphoprotein essential for the initiation of Ebola virus transcription. In this work, we have studied the effect of mutations in VP30 phosphorylation sites on the ebolavirus replication cycle by using a reverse genetics system. We demonstrate that VP30 is involved in reinitiation of gene transcription and that this activity is affected by mutations at the phosphorylation sites.
Martínez
Miguel J
MJ
INSERM, U758, Filovirus Laboratory, 21 Av. Tony Garnier, 69365 Lyon, Cedex 07, France.
Biedenkopf
Nadine
N
Volchkova
Valentina
V
Hartlieb
Bettina
B
Alazard-Dany
Nathalie
N
Reynard
Olivier
O
Becker
Stephan
S
Volchkov
Viktor
V
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D029043:Ebolavirus / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D011994:Recombinant Proteins; D014157:Transcription Factors; C479897:VP30 protein, ebola virus; D014764:Viral Proteins