Transcriptomic and metabonomic profiling of obesity-prone and obesity-resistant rats under high fat diet.
Rodents respond to chronic high fat diet in at least two ways: some of them may readily gain body weight and become obese (termed obesity-prone, OP), and others may not (termed obesity-resistant, OR). Transcriptomic and metabonomic profiling of OP and OR rats has been conducted, showing two sets of significantly different phenotypic profiles in response to 16 weeks of high fat diet. We observed significant differences in transcriptional expression of nearly 80 genes, some of which are known to be involved in lipid metabolism, transport, and ketone body production. The different metabolic profiles in liver tissue extracts, serum, and urine between the two phenotypes can be ascribed to the corresponding pathways identified with multivariate statistical analysis, including fatty acid metabolism, Krebs cycle, and amino acid metabolism. The integration of results from transcriptomic and metabonomic studies revealed that the altered metabolic pathways in OP rats may involve the increased activity of sympathetic nervous system and Krebs cycle, an increased production of ketone bodies, and an adaptive regulatory process to store excessive lipids in liver through reverse cholesterol transport process. These biochemical variations at transcriptional and metabolic levels as a result of dietary intervention highlight the significance of combined "omics" strategy in the mechanistic study of obesity and metabolic disorders.
Li
Houkai
H
School of Pharmacy and Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, PRC.
Xie
Zuoquan
Z
Lin
Jingchao
J
Song
Huaiguang
H
Wang
Qi
Q
Wang
Ke
K
Su
Mingming
M
Qiu
Yunping
Y
Zhao
Tie
T
Song
Kai
K
Wang
Xiaoyan
X
Zhou
Mingmei
M
Liu
Ping
P
Zhao
Guoping
G
Zhang
Qinghua
Q
Jia
Wei
W
D000818:Animals; D001835:Body Weight / Q000235:genetics / Q000502:physiology; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D008297:Male; D055442:Metabolome; D008954:Models, Biological; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014158:Transcription, Genetic
D004041:Dietary Fats