title: Dissecting T cell lineage relationships by cellular barcoding.
abstract: T cells, as well as other cell types, are composed of phenotypically and functionally distinct subsets. However, for many of these populations it is unclear whether they develop from common or separate progenitors. To address such issues, we developed a novel approach, termed cellular barcoding, that allows the dissection of lineage relationships. We demonstrate that the labeling of cells with unique identifiers coupled to a microarray-based detection system can be used to analyze family relationships between the progeny of such cells. To exemplify the potential of this technique, we studied migration patterns of families of antigen-specific CD8(+) T cells in vivo. We demonstrate that progeny of individual T cells rapidly seed independent lymph nodes and that antigen-specific CD8(+) T cells present at different effector sites are largely derived from a common pool of precursors. These data show how locally primed T cells disperse and provide a technology for kinship analysis with wider utility.
authors.affiliation: Division of Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
authors.forename: Koen
authors.initials: K
authors.lastname: Schepers
authors.forename: Erwin
authors.initials: E
authors.lastname: Swart
authors.forename: Jeroen W J
authors.initials: JW
authors.lastname: van Heijst
authors.forename: Carmen
authors.initials: C
authors.lastname: Gerlach
authors.forename: Maria
authors.initials: M
authors.lastname: Castrucci
authors.forename: Daoud
authors.initials: D
authors.lastname: Sie
authors.forename: Mike
authors.initials: M
authors.lastname: Heimerikx
authors.forename: Arno
authors.initials: A
authors.lastname: Velds
authors.forename: Ron M
authors.initials: RM
authors.lastname: Kerkhoven
authors.forename: Ramon
authors.initials: R
authors.lastname: Arens
authors.forename: Ton N M
authors.initials: TN
authors.lastname: Schumacher
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D009369:Neoplasms / Q000276:immunology / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods*; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
chemical_list: D015415:Biomarkers