Isolation, characterization and genome sequencing of phage MZTP02 from Bacillus thuringiensis MZ1.
A lysogenic phage, MZTP02, was produced via induction by mitomycin C from Bacillus thuringiensis (B. thuringiensis) strain MZ1. Plaques were about 3 mm in diameter with a small inner zone consisting of new B. thuringiensis colonies. Electron microscopic analysis showed that MZTP02 had a long tail (220 nm x 18 nm) and an icosahedral head (82 nm x 85 nm). MZTP02 was insensitive to organic solvents such as chloroform, and infected six B. thuringiensis strains. Its complete genome contained 15,717 base pairs (bp) with 37.55% G + C content. Two inverted terminal repeats consisting of 40 bp were 65% identical. Twenty putative open reading frames (ORFs) were found in the MZTP02 genome, and nine predicted proteins, including two terminase subunits, portal protein, minor head protein, scaffold protein, two putative membrane proteins, tail component, and minor structural protein, showed similarity to other phage proteins. But six ORFs were unique. The presence of a terminal protein at the 5'-terminus was demonstrated using proteinase K, lambda exonuclease and E. coli exonuclease III to digest the genome DNA. A TMP phylogenetic tree was constructed based on amino acid sequences from ten phages.
Liao
Wei
W
State Key Laboratory of Biocontrol, School of Life Sciences, Institute of Entomology, Sun Yat-sen Zhongshan University, Guangzhou, People's Republic of China. laoweimail@163.com
Song
Shaoyun
S
Sun
Fan
F
Jia
Yanhua
Y
Zeng
Wenhui
W
Pang
Yi
Y
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017103:Bacillus Phages / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D001413:Bacillus thuringiensis / Q000821:virology*; D001482:Base Composition; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D023061:Gene Order; D016679:Genome, Viral; D008242:Lysogeny; D046529:Microscopy, Electron, Transmission; D016685:Mitomycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D020079:Terminal Repeat Sequences; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure
D000900:Anti-Bacterial Agents; D004279:DNA, Viral; D014764:Viral Proteins; D016685:Mitomycin