[Emergence of the integron-positive multi-drug resistant strain of Acinetobacter baumanii in Russian hospitals].
OBJECTIVE
To detect the integron-positive strains among nosocomial Acinetobacter spp. and to determine their relationship on the genotype level.


METHODS
Amplification by polymerase chain reaction using primers specific to sequences of the class 1 and 2 intergrons on the genomic DNA template followed by restriction fragments length polymorphism analysis as well as RAPD-genotyping of the integron-positive strains were performed.


RESULTS
Fact of spreading of Acinetobacter baumanii strain containing class 1 integron in medical centers was established. The composition of the integron's gene cassettes was analogous to worldwide (including epidemic) types.


CONCLUSIONS
Molecular genetic analysis of the mobilized structures (integrons) under the standardization of used approaches are suitable for the surveillance for circulation of epidemic strains of nosocomial pathogens.
Solomennyĭ
A P
AP
Maksimov
A Iu
AIu
Saralov
A I
AI
Iafaev
R Kh
RKh
Goncharov
A E
AE
Keropian
E A
EA
Multykh
I G
IG
D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D024901:Drug Resistance, Multiple, Bacterial; D004784:Environmental Monitoring; D062665:Epidemiological Monitoring; D006801:Humans; D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D012426:Russia / Q000453:epidemiology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial