title: Decline of serum HBV DNA and no change apportioned by the same hepatic parenchyma cell volume from hepatic fibrosis stage 1 to stage 4 during the natural history of chronic hepatitis B.
abstract: During the initial phase of chronic hepatitis B virus (HBV) infection, serum HBV DNA levels are high. Contrarily, fibrosis, cirrhosis and hepatocellular carcinoma have been found in patients with lower serum HBV DNA levels. The aim of this study is to clarify HBV DNA level dynamics of serum apportioned by the same hepatic parenchyma cell volume (HPCV) in hepatic fibrosis stages 1-4 during the natural history of chronic hepatitis B. Serum HBV DNA levels were evaluated by real-time polymerase chain reaction. Further, serum HBV DNA levels were apportioned by and compared with the same HPCV in hepatic fibrosis stages 1-4, respectively. Serum HBV DNA levels were 8.91 x 10(6) +/- 4.37 x 10(1), 8.13 x 10(6) +/- 7.41 x 10(1), 9.55 x 10(5) +/- 1.02 x 10(2), and 4.07 x 10(5) +/- 7.24 x 10(1) copies/ml, respectively; there were differences among hepatic fibrosis stages 1-4 (p < 0.021-0.000). However, serum HBV DNA levels apportioned by the same volume of hepatic parenchyma cells in hepatic fibrosis stages 1-4 were 3.47 x 10(10) +/- 8.71 x 10(2), 1.02 x 10(11) +/- 9.55 x 10(2), 1.41 x 10(10) +/- 2.57 x 10(3), and 3.72 x 10(10) +/- 3.02 x 10(3) with HPCV proportions 65.9, 62.7, 58.9, and 53.3%, respectively; there were no differences among hepatic fibrosis stages 1-4 (p > 0.203-0.967).Following the progression of hepatic fibrosis from stage 1 to 4, ongoing decline of HPCV is responsible for a declining trend of serum HBV DNA levels.
authors.affiliation: Department of Infectious Diseases, The Third Affiliated Hospital, Sun Yat-sen University, Shipai, Guangzhou, PR China. kwm1999@163.com
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Ke
authors.forename: Shi-Bin
authors.initials: SB
authors.lastname: Xie
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Yu
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Lai
authors.forename: Da-Qiu
authors.initials: DQ
authors.lastname: He
authors.forename: Xiao-He
authors.initials: XH
authors.lastname: Li
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Gao
authors.forename: Ying
authors.initials: Y
authors.lastname: Ke
authors.forename: Pei-Jia
authors.initials: PJ
authors.lastname: Chen
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006515:Hepatitis B virus / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000473:pathology* / Q000821:virology*; D006801:Humans; D008103:Liver Cirrhosis / Q000473:pathology*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012720:Severity of Illness Index; D019562:Viral Load
chemical_list: D004279:DNA, Viral