GPR12 selections of the metabolites from an endophytic Streptomyces sp. associated with Cistanches deserticola.
An endophytic Streptomyces sp. (AC-2) was isolated from the root of Cistanches deserticola Y.C.Ma.. Chemical investigations of the culture broth of AC-2 afforded fifteen compounds including K1115 A (1), tyrosol (2), phenylethylamine derivatives (3, 4), cyclic dipeptides (5-8), nucleosides and their aglycones (9-13), N-acetyltryptamine (14), and pyrrole-2-carboxylic acid (15). Only tyrosol can promote an increase of intracellular cAMP special on GPR12 transfected cells, such as CHO and HEK293, which means it may be a possible ligand for GPR12.
Lin
Zhen-Jian
ZJ
Key laboratory of Marine Drugs, Chinese Ministry of Education, Qingdao, 266003, People's Republic of China.
Lu
Xiao-Ming
XM
Zhu
Tian-Jiao
TJ
Fang
Yu-Chun
YC
Gu
Qian-Qun
QQ
Zhu
Weiming
W
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004121:Dimethyl Sulfoxide; D005285:Fermentation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000187:drug effects*; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000737:chemistry*; D014162:Transfection
C097350:GPR12 protein, human; D010936:Plant Extracts; D012336:RNA, Ribosomal, 16S; D043562:Receptors, G-Protein-Coupled; C011867:4-hydroxyphenylethanol; D004247:DNA; D000242:Cyclic AMP; D010626:Phenylethyl Alcohol; D004121:Dimethyl Sulfoxide