title: Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy.
abstract: The aim of this work was to establish the effect of the cis9,trans11 conjugated linoleic acid (CLA) isomer on mucosal immunity during early life in rats, a period when mucosal immunoglobulin production is poorly developed, as is also the case in humans. CLA supplementation was performed during three life periods: gestation, suckling, and early infancy. The immune status of supplemented animals was evaluated at two time points: at the end of the suckling period (21-day-old rats) and 1 week after weaning (28-day-old rats). Secretory IgA was quantified in intestinal washes from 28-day-old rats by ELISA technique. IgA, TGFbeta, and PPARgamma mRNA expression was measured in small intestine and colon by real time PCR, using Taqman specific probes and primers. IgA mucosal production was enhanced in animals supplemented with CLA during suckling and early infancy: in 28-day-old rats, IgA mRNA expression was increased in small intestine and colon by approximately 6- and 4-fold, respectively, and intestinal IgA protein by approximately 2-fold. TGFbeta gene expression was independent of age and type of tissue considered, and was not modified by dietary CLA. Gene expression of PPARgamma, a possible mediator of CLA's effects was also upregulated in animals receiving CLA during early life. In conclusion, dietary supplementation with CLA during suckling and extended to early infancy enhances development of the intestinal immune response in rats.
authors.affiliation: F. J. Pérez-Cano and C. Ramírez-Santana contributed equally to this work; Department of Physiology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain. Electronic address: franciscoperez@ub.edu.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Pérez-Cano
authors.affiliation: F. J. Pérez-Cano and C. Ramírez-Santana contributed equally to this work; Department of Physiology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Ramírez-Santana
authors.affiliation: Department of Physiology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Molero-Luís
authors.affiliation: Department of Physiology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
authors.forename: Margarida
authors.initials: M
authors.lastname: Castell
authors.affiliation: Ordesa Group, Research Department, Scientific Park of Barcelona, Barcelona, Spain CIBER Epidemiología y Salud Pública (CIBERESP), Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Rivero
authors.affiliation: Department of Physiology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Castellote
authors.affiliation: Department of Physiology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
authors.forename: Àngels
authors.initials: À
authors.lastname: Franch
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D003106:Colon / Q000033:anatomy & histology / Q000276:immunology; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D019587:Dietary Supplements; D005260:Female; D015870:Gene Expression; D018928:Immunity, Mucosal / Q000235:genetics; D007071:Immunoglobulin A, Secretory / Q000235:genetics / Q000378:metabolism*; D007421:Intestine, Small / Q000033:anatomy & histology / Q000276:immunology; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D047495:PPAR gamma / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000235:genetics
chemical_list: D004042:Dietary Fats, Unsaturated; D007071:Immunoglobulin A, Secretory; D044243:Linoleic Acids, Conjugated; D047495:PPAR gamma; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C503589:cis-9, trans-11-conjugated linoleic acid