title: Methods for the purification of HQ-tagged proteins.
abstract: The HQ (H = histidine, Q = glutamine) tag is a small fusion tag that can be isolated using immobilized metal affinity columns. HQ-tagged proteins can be expressed and purified from bacterial cells under native and denaturing conditions, mammalian cells, insect cells, wheat germ and rabbit reticulocyte. Furthermore, HQ-tagged proteins can be purified using magnetic or non-magnetic resins in multiple formats from small to large-scale and manual or automated. In this chapter, we have described various protocols for the purification of HQ-tagged proteins.
authors.affiliation: Proteomics R&D, Promega Corporation, Fitchburg, WI, USA.
authors.forename: Becky
authors.initials: B
authors.lastname: Godat
authors.forename: Laurie
authors.initials: L
authors.lastname: Engel
authors.forename: Natalie A
authors.initials: NA
authors.lastname: Betz
authors.forename: Tonny M
authors.initials: TM
authors.lastname: Johnson
mesh_terms: D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D002846:Chromatography, Affinity / Q000379:methods*; D005973:Glutamine / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D013058:Mass Spectrometry; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011993:Recombinant Fusion Proteins; D005973:Glutamine; D006639:Histidine