title: Pholasin luminescence is enhanced by addition of dehydrocoelenterazine.
abstract: Pholasin is a bioluminescent photoprotein of Pholas dactylus. Pholasin is a commercially available photoprotein used to measure intracellular reactive oxygen species. Although extensive efforts have been carried out to determine the chemical structure of the prosthetic (chromophore) group, it still remains unclear to date. Herein, we report the enhancement of pholasin luminescence by the addition of dehydrocoelenterazine, which is organic substance of luminous squids' photoprotein.
authors.affiliation: Chemical Instrument division, Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan. kuse@cic.nagoya-u.ac.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Kuse
authors.forename: Eiko
authors.initials: E
authors.lastname: Tanaka
authors.forename: Toshio
authors.initials: T
authors.lastname: Nishikawa
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods; D049832:Decapodiformes; D015195:Drug Design; D049454:Firefly Luciferin / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry* / Q000494:pharmacology; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry* / Q000494:pharmacology; D008163:Luminescent Measurements; D008164:Luminescent Proteins; D008956:Models, Chemical; D011719:Pyrazines / Q000737:chemistry* / Q000494:pharmacology; D017382:Reactive Oxygen Species; D013997:Time Factors
chemical_list: D007093:Imidazoles; D049408:Luminescent Agents; D008164:Luminescent Proteins; D011719:Pyrazines; D017382:Reactive Oxygen Species; C116816:dehydrocoelenterazine; D049454:Firefly Luciferin; C052157:pholasin