title: Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are small (approximately 22 nucleotides) regulatory RNAs which play fundamental roles in many human diseases, including cancer. There is no report on the miRNA expression profile of retinoblastoma.
  
  
  METHODS
  This work was undertaken to identify differentially expressed miRNAs in human retinoblastoma tissues by microRNA microarray technique, and some miRNAs were verified using northern blot analysis and the in situ hybridization method.
  
  
  RESULTS
  A cluster of microRNAs was identified as highly expressed in retinoblastoma, including hsa-miR-494, hsa-let-7e, hsa-miR-513-1, hsa-miR-513-2, hsa-miR-518c*, hsa-miR-129-1, hsa-miR-129-2, hsa-miR-198, hsa-miR-492, hsa-miR-498, hsa-miR-320, hsa-miR-503, and hsa-miR-373*.
  
  
  CONCLUSIONS
  These miRNAs are the first to be reported for human retinoblastoma and may play significant roles in regulating tumor genesis.
authors.affiliation: Peking Union Medical College, Tsinghua University, Beijing, China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Juhua
authors.initials: J
authors.lastname: Yang
authors.forename: Jianhong
authors.initials: J
authors.lastname: Lin
authors.forename: Nan
authors.initials: N
authors.lastname: Yao
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jianlong
authors.initials: J
authors.lastname: Zheng
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xu
authors.forename: Jin Q
authors.initials: JQ
authors.lastname: Cheng
authors.forename: Jian-Yin
authors.initials: JY
authors.lastname: Lin
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
mesh_terms: D000328:Adult; D015152:Blotting, Northern; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012175:Retinoblastoma / Q000235:genetics*
chemical_list: C527114:MIRN373 microRNA, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human