Division protein interaction web: identification of a phylogenetically conserved common interactome between Streptococcus pneumoniae and Escherichia coli.
The ability of each of the 11 Streptococcus pneumoniae division proteins to interact with itself and with each of the remaining proteins was studied in 66 combinations of protein pairs, using a bacterial two-hybrid system. Interactions (homo- or hetero-dimerizations) were detected between 37 protein pairs, whereas 29 protein pairs did not interact. In some cases, positive interactions of the S. pneumoniae proteins were confirmed by co-immunoprecipitation experiments in Escherichia coli. Comparison between the S. pneumoniae division protein interaction web and that of E. coli, the only micro-organisms for which the whole division interactome has been described systematically, was also performed. At least nine division proteins, ZapA, FtsZ, FtsA, FtsK, FtsQ/DivIB, FtsB/DivIC, FtsL, FtsI and FtsW, are believed to have a conserved function between these bacteria and thus we may say that a significant part of the interactions are conserved. Out of 45 protein pairs tested in both bacteria, 30 showed the same behaviour: 23 interacted while seven did not. In agreement with these results, cross-interactions between S. pneumoniae proteins and the corresponding E. coli orthologues were observed. Taken together, these results suggest a phylogenetically conserved minimal common interactome of the division proteins.
Maggi
Silvia
S
Dipartimento di Biologia, Università Tor Vergata, Roma, Italy.
Massidda
Orietta
O
Dipartimento di Scienze e Tecnologie Biomediche, Sez. Microbiologia Medica, Cagliari, Italy.
Luzi
Giuseppe
G
Dipartimento di Medicina Interna, Facoltà di Medicina, Università La Sapienza, Roma, Italy.
Fadda
Daniela
D
Dipartimento di Scienze e Tecnologie Biomediche, Sez. Microbiologia Medica, Cagliari, Italy.
Paolozzi
Luciano
L
Dipartimento di Biologia, Università Tor Vergata, Roma, Italy.
Ghelardini
Patrizia
P
Istituto di Biologia e Patologia Molecolare del CNR, Roma, Italy.|Dipartimento di Biologia, Università Tor Vergata, Roma, Italy.
D001426:Bacterial Proteins / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D002455:Cell Division; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D047468:Immunoprecipitation; D010802:Phylogeny; D010957:Plasmids; D025941:Protein Interaction Mapping; D020543:Proteome / Q000235:genetics; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000235:genetics*; D020798:Two-Hybrid System Techniques
D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D020543:Proteome