Recombinant collagen for animal product-free dextran microcarriers.
Manufacturers of vaccines and other biologicals are under increasing pressure from regulatory agencies to develop production methods that are completely animal-component-free. In order to comply with this demand, alternative cell culture substrates to those now on the market, primarily collagen or gelatin, must be found. In this paper, we have tested a number of possible substitutes including recombinant collagen, a 100-kDa recombinant gelatin fragment and a peptide derived from a cell-binding region of type I collagen. The small 15-amino acid peptide did not support attachment of human fibroblasts in monolayer culture. The 100-kDa gelatin fragment supported cell attachment in monolayer culture, but was significantly less active than intact porcine gelatin. Recombinant type I collagen was as successful in promoting cell attachment as native collagen, and both were more effective than porcine gelatin. Based on these data, dextran microspheres were treated with the same attachment proteins--porcine gelatin, native collagen, or recombinant collagen. The same trends were observed as in monolayer culture. Concentrations of the recombinant collagen (as well as native collagen) supported cell attachment on dextran microspheres at concentrations as low as 0.01 microg/cm(2). Treatment of the dextran with a low level of polyethylenimine, a cationic moiety, further enhanced attachment when used in conjunction with the low concentration of recombinant collagen. Where there was increased cell attachment, increased proliferation followed. We are confident, based on these findings, that a fully recombinant substitute could replace gelatin in current microcarrier preparations without losing the cell growth benefits provided by the native protein.
Dame
Michael K
MK
Department of Pathology, The University of Michigan, Ann Arbor, MI 48109, USA. mdame@med.umich.edu
Varani
James
J
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000378:metabolism* / Q000494:pharmacology; D003911:Dextrans / Q000378:metabolism*; D004337:Drug Carriers / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008863:Microspheres; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism; D011094:Polyethyleneimine; D011137:Polystyrenes; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism* / Q000494:pharmacology; D034421:Sus scrofa
D003911:Dextrans; D004337:Drug Carriers; D010455:Peptides; D011137:Polystyrenes; D011994:Recombinant Proteins; D011094:Polyethyleneimine; D003094:Collagen