title: DCUN1D3, a novel UVC-responsive gene that is involved in cell cycle progression and cell growth.
abstract: DCUN1D3 (DCN1, defective in cullin neddylation 1, domain containing 3) was found during the process of high throughput screening of novel human genes associated with serum response element (SRE) pathway activation. The DCUN1D3 gene is highly conserved among vertebrates. Human DCUN1D3 complementary DNA (cDNA) encodes 304 amino acids with an apparent molecular mass of 34 kDa. However, there has been no report about the function of DCUN1D3. This study detected that DCUN1D3 was broadly expressed in several tumor tissues and cultured cell lines; however, UVC irradiation of different doses significantly increased DCUN1D3 expression level in these cancer cell lines. Over-expression of the DCUN1D3 inhibits cell growth in HeLa. When the DCUN1D3 gene was silenced by siRNA in UVC-treated HeLa, the cell cycle in S phase was remarkably blocked; furthermore, the UVC-induced cell death was inhibited. In addition, DCUN1D3 localized mainly in the cytoplasm and perinuclear, but after UVC treatment, the DCUN1D3 gradually entered the nucleus. All the results above indicate that DCUN1D3 is a novel UVC-response gene involved in cell cycle regulation and cell survival.
authors.affiliation: Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Ma
authors.forename: Taiping
authors.initials: T
authors.lastname: Shi
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Lina
authors.initials: L
authors.lastname: Wu
authors.forename: Fanlei
authors.initials: F
authors.lastname: Hu
authors.forename: PengFei
authors.initials: P
authors.lastname: He
authors.forename: WeiWei
authors.initials: W
authors.lastname: Deng
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Quansheng
authors.initials: Q
authors.lastname: Song
authors.forename: Dalong
authors.initials: D
authors.lastname: Ma
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics* / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D018797:Cell Cycle Proteins; C534519:DCUN1D3 protein, human; D018076:DNA, Complementary; D015513:Oncogene Proteins; D034741:RNA, Small Interfering