Electron beam triggered, free radical polymerization-derived monolithic capillary columns for high-performance liquid chromatography.
Monolithic capillary columns were prepared via electron beam triggered free radical polymerization within the confines of 0.2 and 0.1mm I.D. capillary columns using ethyl methacrylate and trimethylolpropane triacrylate as monomers as well as 2-propanol, 1-dodecanol and toluene as porogenic system. The influence of column diameter on reproducibility and separation performance was investigated. For evaluation, a protein standard consisting of five proteins in the range of 5800-66,000 g mol(-1) was used. Reproducibility was checked by determining the relative standard deviations in retention times, peak widths at half height, asymmetry and resolution. Excellent run-to-run reproducibility was found for both 0.2 and 0.1mm I.D. columns; batch-to-batch reproducibility was good for both column types. In order to enhance the non-polar character of the monolithic columns, lauryl methacrylate-based capillary columns were prepared. These were successfully used for the separation of proteins and a cytochrome c digest.
Schlemmer
Bettina
B
Leibniz-Institut für Oberflächenmodifizierung e.V., Permoserstrasse 15, D-04318 Leipzig, Germany.
Bandari
Rajendar
R
Rosenkranz
Lutz
L
Buchmeiser
Michael R
MR
D019840:2-Propanol / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D007851:Dodecanol / Q000737:chemistry; D004583:Electrons; D005609:Free Radicals / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis*; D011506:Proteins / Q000302:isolation & purification; D015203:Reproducibility of Results
D000179:Acrylates; D005609:Free Radicals; D008689:Methacrylates; D011108:Polymers; D011506:Proteins; D007851:Dodecanol; C027993:trimethylolpropane triacrylate; D019840:2-Propanol