title: Decreased lipin 1 beta expression in visceral adipose tissue is associated with insulin resistance in polycystic ovary syndrome.
abstract: OBJECTIVE
  In polycystic ovary syndrome (PCOS), insulin resistance (IR) appears with high prevalence and represents the major cause of cardiometabolic complications. Lipin 1beta regulates lipid metabolism and augments insulin sensitivity. The impact of lipin 1beta expression in visceral and subcutaneous adipose tissue of PCOS patients on IR was studied for the first time.
  
  
  METHODS
  Eighty-five PCOS patients and 44 controls were enrolled for subcutaneous tissue biopsy, of whom 25 patients and 30 controls also underwent visceral adipose tissue biopsy. Gene expression of lipin 1beta was measured, together with that of peroxisome proliferator-activated receptor gamma, lipoprotein lipase, hormone-sensitive lipase, adiponectin and glucose transporter 4 in subcutaneous and visceral adipose tissue. Markers of obesity, IR and PCOS were also measured.
  
  
  RESULTS
  In PCOS patients, lipin 1beta expression in both adipose depots was lower than in controls: 0.76 (0.67-0.84) vs 1.16 (0.90-1.43) for visceral and 0.91 (0.73-1.10) vs 1.30 (1.03-1.57) for s.c. depot (both P<10(-4)). The difference remained significant after adjustment for body mass index (BMI) and also when comparing only lean patients with lean controls. In PCOS patients, visceral adipose lipin 1beta expression correlated negatively with homeostasis model assessment-IR (r=-0.474, P=0.017), BMI (r=-0.511, P=0.009) and waist circumference (r=-0.473, P=0.017), waist circumference remaining significant (P=0.027) in multiple regression. Subcutaneous lipin 1beta expression in PCOS correlated negatively with BMI, waist circumference and plasma triglycerides, and positively with high density lipoprotein-cholesterol. Subcutaneous, but not visceral lipin 1beta expression, correlated positively with the studied genes.
  
  
  CONCLUSIONS
  Lipin 1beta appears to be involved in the pathogenesis of IR in PCOS.
authors.affiliation: Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, SI-1000 Ljubljana, Slovenia.
authors.forename: Barbara
authors.initials: B
authors.lastname: Mlinar
authors.forename: Marija
authors.initials: M
authors.lastname: Pfeifer
authors.forename: Eda
authors.initials: E
authors.lastname: Vrtacnik-Bokal
authors.forename: Mojca
authors.initials: M
authors.lastname: Jensterle
authors.forename: Janja
authors.initials: J
authors.lastname: Marc
mesh_terms: D000328:Adult; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology*; D050152:Intra-Abdominal Fat / Q000502:physiology*; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics; D010711:Phosphatidate Phosphatase; D011085:Polycystic Ovary Syndrome / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D009687:Nuclear Proteins; C485999:LPIN1 protein, human; D010711:Phosphatidate Phosphatase