title: Determination of (+)-3,3',5,5'-Tetraiodo-L-thyronine (L-T4) in Serum and pharmaceutical formulations using a sequential injection analysis/immunosensor system.
abstract: A sequential injection analysis/immunosensor system is proposed for the analysis of thyroxine (L-T4) in serum and in pharmaceutical formulations with a rate of 75 samples/hour. The immunosensor design is based on the physical immobilization of anti-L-T4 in carbon paste. The working concentration range of the immunosensor in the sequential injection analysis system is between 36 and 1080 ng/mL with a limit of detection of 24.6 ng/mL. The system is very reliable and very easy to design and operate.
authors.affiliation: Laboratory of Electrochemistry Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, Bucharest, Romania. iustinavanstaden@yahoo.com
authors.forename: Raluca-Ioana
authors.initials: RI
authors.lastname: Stefan-van Staden
authors.forename: Jacobus Frederick
authors.initials: JF
authors.lastname: van Staden
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
authors.forename: Marius Constantin
authors.initials: MC
authors.lastname: Mirica
authors.forename: Ionel
authors.initials: I
authors.lastname: Balcu
authors.forename: Nicolae
authors.initials: N
authors.lastname: Mirica
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015374:Biosensing Techniques; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D007118:Immunoassay; D004364:Pharmaceutical Preparations / Q000032:analysis; D013974:Thyroxine / Q000032:analysis / Q000097:blood* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D004364:Pharmaceutical Preparations; D013974:Thyroxine