Immunoaffinity chromatography.
Immunonaffinity chromatography is a powerful technique for rapid purification of proteins. In a single-step purification, it is possible to purify proteins for testing in model systems and for conducting enzyme kinetic studies. Because the immunoaffinity-purified proteins are typically > 90-95% pure, depending on the starting material, interference from remaining contaminants is rare. This method describes an immunoaffinity chromatography technique for purifying proteins from over-expression in mammalian cell culture. The immobilization of the monoclonal antibody or polyclonal antiserum is presented. Conditions for purifying up to milligram quantities of protein are given, including a representative chromatogram.
Gallant
Stuart R
SR
Process Sciences Department, BioMarin Pharmaceutical Inc, Novato, CA, USA.
Koppaka
Vish
V
Zecherle
Nick
N
D000818:Animals; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins