title: Estimating kinetic and thermodynamic parameters from single molecule enzyme-inhibitor interactions.
abstract: We report the application of recently developed microscopic models to estimate the apparent kinetic and thermodynamic parameters in a single molecule force spectroscopy study of the carbonic anhydrase enzyme and a complementary sulfonamide inhibitor. The most probable rupture force for the enzyme-inhibitor interaction shows a nonlinear dependency on the log-loading rate. Estimates for the kinetic and thermodynamic parameters were obtained by fitting the nonlinear dependency to linear cubic potential and cusp potential models and compared to the standard Bell-Evans model. The reliability of the estimated parameters was verified by modeling the experimental rupture force distributions by the theoretically predicted distributions at rupture. We also report that linkers that are attached to the enzyme and inhibitor show appreciable effects on the apparent kinetic and thermodynamic parameters.
authors.affiliation: Department of Chemistry & Biochemistry, Calvin College, Grand Rapids, Michigan 49546, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Porter-Peden
authors.forename: Sarah G
authors.initials: SG
authors.lastname: Kamper
authors.forename: Mark Vander
authors.initials: MV
authors.lastname: Wal
authors.forename: Ronald
authors.initials: R
authors.lastname: Blankespoor
authors.forename: Kumar
authors.initials: K
authors.lastname: Sinniah
mesh_terms: D000818:Animals; D001703:Biophysics / Q000379:methods; D024402:Carbonic Anhydrase II / Q000737:chemistry*; D002417:Cattle; D004791:Enzyme Inhibitors / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D007700:Kinetics; D018625:Microscopy, Atomic Force / Q000379:methods*; D008956:Models, Chemical; D015233:Models, Statistical; D015394:Molecular Structure; D055672:Static Electricity; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D004791:Enzyme Inhibitors; D004798:Enzymes; D004800:Enzymes, Immobilized; D024402:Carbonic Anhydrase II