title: Complete nucleotide sequence analysis of a Dengue-1 virus isolated on Easter Island, Chile.
abstract: Dengue-1 viruses responsible for the dengue fever outbreak in Easter Island in 2002 were isolated from acute-phase sera of dengue fever patients. In order to analyze the complete genome sequence, we designed primers to amplify contiguous segments across the entire sequence of the viral genome. RT-PCR products obtained were cloned, and complete nucleotide and deduced amino acid sequences were determined. This report constitutes the first complete genetic characterization of a DENV-1 isolate from Chile. Phylogenetic analysis shows that an Easter Island isolate is most closely related to Pacific DENV-1 genotype IV viruses.
authors.affiliation: Genética Molecular, Instituto de Salud Pública de Chile, Santiago, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Cáceres
authors.forename: V
authors.initials: V
authors.lastname: Yung
authors.forename: P
authors.initials: P
authors.lastname: Araya
authors.forename: J
authors.initials: J
authors.lastname: Tognarelli
authors.forename: E
authors.initials: E
authors.lastname: Villagra
authors.forename: L
authors.initials: L
authors.lastname: Vera
authors.forename: J
authors.initials: J
authors.lastname: Fernández
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics* / Q000302:isolation & purification*; D004196:Disease Outbreaks; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011114:Polynesia / Q000453:epidemiology; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D012367:RNA, Viral; D014764:Viral Proteins