title: Derivation of cranial neural crest-like cells from human embryonic stem cells.
abstract: The neural crest is a transient population of multipotent progenitors contributing to a diverse array of tissues throughout the vertebrate embryo. Embryonic stem (ES) cells are able to form embryoid body and spontaneously differentiate to various lineages, following a reproducible temporal pattern of development that recapitulates early embryogenesis. Embryoid bodies were triturated and the dissociated cells were processed for fluorescence-activated cell sorting (FACS), and more than 1% of cells were identified as frizzled-3(+)/cadherin-11(+). Expression of marker genes associated with various terminal fates was detected for chondrocytes, glia, neurons, osteoblasts and smooth muscles, indicating that the FACS-sorted frizzled-3(+)/cadherin-11(+) cells were multipotent progenitor cells capable of differentiating to fates associated with cranial neural crest. Moreover, the sorted cells were able to self-renew and maintain multipotent differentiation potential. The derivation of cranial neural crest-like multipotent progenitor cells from ES cells provides a new tool for cell lineage analysis of neural crest in vitro.
authors.affiliation: University of Southern California School of Dentistry, Center for Craniofacial Molecular Biology, 2250 Alcazar Street, CSA Room 103, Los Angeles, CA 90033, USA. yzhou@usc.edu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Malcolm L
authors.initials: ML
authors.lastname: Snead
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D015820:Cadherins / Q000032:analysis / Q000096:biosynthesis; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics; D053595:Embryonic Stem Cells / Q000378:metabolism / Q000502:physiology*; D005434:Flow Cytometry; D051157:Frizzled Receptors / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000032:analysis / Q000096:biosynthesis; D012886:Skull / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D015820:Cadherins; C105574:FZD3 protein, human; D051157:Frizzled Receptors; D043562:Receptors, G-Protein-Coupled; C086960:osteoblast cadherin