title: The detection of sLFA-3 in plasma of patients with hemorrhagic fever with renal syndrome.
abstract: Hemorrhagic fever with renal syndrome (HFRS) is an acute viral disease characterized by endothelial dysfunction. The cellular immune response, especially the virus-specific CD8+ T lymphocytes, is known to attack vascular endothelial cells (VEC) and to contribute to the diffuse damage and penetrability increasing of VEC. Lymphocyte function associated antigen 3 (LFA-3) is expressed on T lymphocytes and VEC, which is contributed to the activation of T lymphocytes. The expression of LFA-3 on the activated T lymphocytes and VEC is highly increased, which can exfoliate into plasma to increase the level of soluble LFA-3 (sLFA-3) in plasma. So the change of sLFA-3 levels is correlated with the activation of T lymphocytes. In this study we detected the levels of sLFA-3 in plasma of patients with HFRS. We examined the levels of sLFA-3 in plasma samples collected from 53 HFRS patients by double antibody sandwich ELISA. We found variable, but persistently elevated levels of sLFA-3 throughout the various phases and types of the HFRS disease, which suggest that sLFA-3 levels have correlation with disease stages. Moreover, elevated sLFA-3 levels are closely correlated to the severity of HFRS and the degree of kidney damage.
authors.affiliation: Department of Pathogenic Microbiology and Immunology, Medical School of Xi'an Jiaotong University, 710061, Xi'an, China. xieming@mail.xjtu.edu.cn
authors.forename: Ming
authors.initials: M
authors.lastname: Xie
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Lin-Jing
authors.initials: LJ
authors.lastname: He
authors.forename: Bao-Tai
authors.initials: BT
authors.lastname: Qi
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Wang
authors.forename: Hui-Xun
authors.initials: HX
authors.lastname: Ren
mesh_terms: D001806:Blood Urea Nitrogen; D018968:CD58 Antigens / Q000097:blood*; D003404:Creatinine / Q000652:urine; D042783:Endothelial Cells / Q000502:physiology; D006480:Hemorrhagic Fever with Renal Syndrome / Q000097:blood* / Q000276:immunology; D006801:Humans
chemical_list: D018968:CD58 Antigens; D003404:Creatinine