title: Multiplexed screening of cellular uptake of gold nanoparticles using laser desorption/ionization mass spectrometry.
abstract: Gold nanoparticles (AuNPs) are highly promising candidates as drug delivery agents into cells of interest. We describe for the first time the multiplexed analysis of nanoparticle uptake by cells using mass spectrometry. We demonstrate that the cellular uptake of functionalized gold nanoparticles with cationic or neutral surface ligands can be readily determined using laser desorption/ionization mass spectrometry of cell lysates. The surface ligands have "mass barcodes" that allow different nanoparticles to be simultaneously identified and quantified at levels as low as 30 pmol. Using this method, we find that subtle changes to AuNP surface functionalities can lead to measurable changes in cellular uptake propensities.
authors.affiliation: Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors.forename: Zheng-Jiang
authors.initials: ZJ
authors.lastname: Zhu
authors.forename: Partha S
authors.initials: PS
authors.lastname: Ghosh
authors.forename: Oscar R
authors.initials: OR
authors.lastname: Miranda
authors.forename: Richard W
authors.initials: RW
authors.lastname: Vachet
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
mesh_terms: D000818:Animals; D019556:COS Cells; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D016503:Drug Delivery Systems; D006046:Gold / Q000737:chemistry / Q000493:pharmacokinetics*; D008024:Ligands; D053768:Metal Nanoparticles / Q000032:analysis / Q000737:chemistry*; D008970:Molecular Weight; D010316:Particle Size; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
chemical_list: D008024:Ligands; D006046:Gold