Risk genotypes and haplotypes of the GLUT1 gene for type 2 diabetic nephropathy in the Tunisian population.
OBJECTIVE
Diabetic nephropathy (DN) is a long-term complication of both type 1 and type 2 diabetes. Genetic studies on DN have been of little help so far, since several genetic association studies have shown conflicting results. Here we report the findings of a case-control study on five SNPs in the glucose transporter 1 (GLUT1) gene. The study investigated the association of five GLUT1 genotypes and haplotypes with DN.


METHODS
All subjects, 126 DN (cases) and 273 type 2 diabetes (controls), were genotyped using the polymerase chain reaction restriction fragment length polymorphism.


RESULTS
The TT and the AA genotypes of the Haell and Enh2 SNP1, increased the risk of DN. The study also identified CGT as the highest risk haplotype (4.4-fold) followed by CAT with an increased risk of DN of 2.6-fold.


CONCLUSIONS
The GLUT1 gene confers susceptibility to DN in type 2 diabetes patients in the Tunisian population.
Makni
K
K
Research Unit Target for Diagnosis and Therapeutic in the Human Pathology, Center of Biotechnology of Sfax, Tunisia. kaouthar@lycos.com
Jarraya
F
F
Rebaï
M
M
Mnif
F
F
Boudawara
M
M
Hamza
N
N
Rekik
N
N
Abid
M
M
Hachicha
J
J
Granier
C
C
Rebaï
A
A
Ayadi
H
H
D000368:Aged; D044383:Black People / Q000235:genetics*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000235:genetics*; D003928:Diabetic Nephropathies / Q000150:complications / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D051272:Glucose Transporter Type 1 / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D014416:Tunisia
D051272:Glucose Transporter Type 1; C495677:SLC2A1 protein, human