Persistence of human parvovirus B19 in multipotent mesenchymal stromal cells expressing the erythrocyte P antigen: implications for transplantation.
Multipotent mesenchymal stromal cells (MSCs) are used to improve the outcome of hematopoietic stem cell transplantation (HCST) and in regenerative medicine. MSCs may harbor persistent viruses that may compromise their clinical benefit, however. Retrospectively screened, 1 of 20 MSCs from healthy donors contained parvovirus B19 (B19) DNA. MSCs express the B19 receptor (P antigen/globoside) and a co-receptor (Ku 80) and can transmit B19 to bone marrow cells in vitro, suggesting that the virus can persist in the marrow stroma of healthy individuals. Two patients undergoing HSCT received the B19-positive MSCs as treatment for graft-versus-host disease; neither developed viremia nor symptomatic B19 infection. These findings demonstrate for the first time that persistent B19 in MSCs can infect hematopoietic stem cells and underscore the importance of monitoring B19 transmission by MSC products.
Sundin
Mikael
M
Division of Clinical Immunology and Transfusion Medicine, Karolinska Institutet, Stockholm, Sweden. Electronic address: mikael.sundin@ki.se.
Lindblom
Anna
A
Unit of Infectious Diseases, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Solna, Stockholm, Sweden.
Örvell
Claes
C
Division of Clinical Virology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden; Laboratory of Clinical Virology, Karolinska University Hospital Huddinge, Stockholm, Sweden.
Barrett
A John
AJ
Allogeneic Stem Cell Transplantation Section, Hematology Branch, National Institutes of Health, Bethesda, Maryland.
Sundberg
Berit
B
Division of Clinical Immunology and Transfusion Medicine, Karolinska Institutet, Stockholm, Sweden.
Watz
Emma
E
Division of Clinical Immunology and Transfusion Medicine, Karolinska Institutet, Stockholm, Sweden.
Wikman
Agneta
A
Division of Clinical Immunology and Transfusion Medicine, Karolinska Institutet, Stockholm, Sweden.
Broliden
Kristina
K
Unit of Infectious Diseases, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Solna, Stockholm, Sweden.
Le Blanc
Katarina
K
Division of Clinical Immunology and Transfusion Medicine, Karolinska Institutet, Stockholm, Sweden; Hematology Centre, Karolinska University Hospital Huddinge, Stockholm, Sweden.
D018920:Coculture Techniques; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D008403:Mass Screening; D045164:Mesenchymal Stem Cell Transplantation / Q000009:adverse effects; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000821:virology*; D010127:P Blood-Group System / Q000276:immunology*; D010322:Parvoviridae Infections / Q000635:transmission*; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D012189:Retrospective Studies; D014019:Tissue Donors
D004279:DNA, Viral; D010127:P Blood-Group System