title: Factors affecting the removal of aflatoxin M1 from food model by Lactobacillus and Bifidobacterium strains.
abstract: This paper describes the ability of six dairy strains of Lactobacillus and Bifidobacterium to remove aflatoxin M1 (AFM 1) from phosphate-buffered saline (PBS) and reconstituted milk. Bacteria were incubated in both PBS and reconstituted milk containing 5, 10 and 20 ng mL(-1) for 0, 4 and 24 h at 37 degrees C. After centrifugation the concentration of AFM 1 was determined in the supernatant fraction using high-performance liquid chromatography. The binding abilities of AFM 1 by viable (10(8) CFU mL(-1)) and heat-killed Lactobacillus and Bifidobacterium strains in PBS ranged from 10.22 to 26.65% and 14.04 to 28.97%, respectively. Similarly, AFM 1-binding capacity in reconstituted milk was found to range from 7.85 to 25.94% and from 12.85 to 27.31% for viable and heat-killed bacteria, respectively within 4 h. While B. bifidum Bb 13 was the best binder, the poorest removal was achieved by L. acidophilus NCC 68. Binding was reversible, and a small proportion of AFM 1 was released back into the solution. The toxin concentration and incubation period had no effect on the removal of AFM 1 by bacteria both in PBS and reconstituted milk.
authors.affiliation: Department of Food Engineering, Hitit University, Corum, Turkey. bulentkabak@hitit.edu.tr
authors.forename: Bulent
authors.initials: B
authors.lastname: Kabak
authors.forename: Isil
authors.initials: I
authors.lastname: Var
mesh_terms: D016607:Aflatoxin M1 / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000517:prevention & control; D057927:Hydrophobic and Hydrophilic Interactions; D007778:Lactobacillus / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D012965:Sodium Chloride; D016607:Aflatoxin M1