Molecular weight and monosaccharide composition of Astragalus polysaccharides.
Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLC-GPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH(2) column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1:3.45 and 1:6.25:17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.
Xu
Du-Juan
DJ
Department of Pharmacy, First Affiliated Hospital of Anhui Medical University, Hefei, 230022. PR China. xudujuan6365@yahoo.com.cn
Xia
Quan
Q
Wang
Jia-Jia
JJ
Wang
Pei-Pei
PP
D027883:Astragalus Plant / Q000737:chemistry*; D002845:Chromatography; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis*; D010936:Plant Extracts; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
D009005:Monosaccharides; D010936:Plant Extracts; D011134:Polysaccharides