title: Clinical severity correlates with impaired barrier in filaggrin-related eczema.
abstract: Mutations in the gene-encoding filaggrin (FLG), a key molecule involved in skin barrier function, have been shown to be a major predisposing factor for atopic dermatitis (AD; eczema). To elucidate the pathomechanisms underlying filaggrin-related AD, we investigated stratum corneum (SC) hydration and transepidermal water loss (TEWL) as parameters of barrier function in AD patients harboring FLG mutations compared to AD patients without any FLG mutation. In filaggrin-related AD, SC hydration was both significantly reduced (P<0.01-0.05) and thicker (P<0.01-0.05) than that in healthy controls. TEWL was demonstrably increased in non-filaggrin AD compared to healthy controls (P<0.01-0.05). The objective score of atopic dermatitis (OSCORAD), a disease clinical severity index, significantly correlated with TEWL (r=0.81, P<0.005), SC hydration (r=-0.65, P<0.05), and SC thickness (r=0.59, P<0.05) in filaggrin-related AD. On the contrary, there was no correlation between these parameters and the OSCORAD in non-filaggrin AD. Furthermore, a significant correlation was obtained between the OSCORAD and specific IgE for house dust (r=0.66, P<0.05), mite allergen (r=0.53, P<0.05), and cat dander (r=0.64, P<0.05) in filaggrin-related AD, but not in non-filaggrin AD. All these data suggest that experimentally demonstrable skin barrier defects due to FLG mutations may play a crucial role in the pathogenesis of AD.
authors.affiliation: Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Ikue
authors.initials: I
authors.lastname: Nemoto-Hasebe
authors.forename: Masashi
authors.initials: M
authors.lastname: Akiyama
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Nomura
authors.forename: Aileen
authors.initials: A
authors.lastname: Sandilands
authors.forename: W H Irwin
authors.initials: WH
authors.lastname: McLean
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D016022:Case-Control Studies; D002648:Child; D004485:Eczema / Q000378:metabolism*; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D000091344:Filaggrin Proteins; D005838:Genotype; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000737:chemistry* / Q000378:metabolism*; D008297:Male; D051379:Mice; D009154:Mutation
chemical_list: C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D007073:Immunoglobulin E