Reversible pH lability of cross-linked vault nanocapsules.
Vaults are ubiquitous, self-assembled protein nanocapsules with dimension in the sub-100 nm range that are conserved across diverse phyla from worms to humans. Their normal presence in humans at a copy number of over 10,000/cell makes them attractive as potential drug delivery vehicles. Toward this goal, bifunctional amine-reactive reagents are shown to be useful for the reversible cross-linking of recombinant vaults such that they may be closed and opened in a controllable manner.
Yu
Marcella
M
Chemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, California 90095, USA.
Ng
Benny C
BC
Rome
Leonard H
LH
Tolbert
Sarah H
SH
Monbouquette
Harold G
HG
D000588:Amines / Q000737:chemistry; D000818:Animals; D001709:Biotechnology / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D016503:Drug Delivery Systems; D004583:Electrons; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D008968:Molecular Conformation; D053769:Nanocapsules / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*
D000588:Amines; D003432:Cross-Linking Reagents; D053769:Nanocapsules