Cleavage sites of the "P3 region" in the nonstructural polyprotein precursor of a dicistrovirus.
Plautia stali intestine virus (PSIV) is a member of the family Dicistroviridae. Dicistroviruses, like picornaviruses, are thought to encode a 3C-like protease; however, the cleavage sites of dicistroviral nonstructural polyprotein precursors are unknown except for those in genome-linked virus protein (VPg) regions. We expressed part of the PSIV polyprotein in Escherichia coli and detected autoprocessed viral proteins. N-terminal sequence analysis of the autoprocessed proteins showed that Q/GWLSW and Q/NGVFD in the PSIV sequence correspond to 2C/3A and 3C/3D cleavage sites in picornaviruses. Alignments of deduced amino acid sequences of the "P3 region" suggest that these cleavage sites can be predicted.
Nakashima
Nobuhiko
N
National Institute of Agrobiological Sciences, Owashi, Tsukuba, Ibaraki, Japan. nakaji@affrc.go.jp
Nakamura
Yuri
Y
D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000378:metabolism*; D010849:Picornaviridae / Q000502:physiology*; D020815:Polyproteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D020815:Polyproteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D010447:Peptide Hydrolases