title: Collagen remodeling and suburethral inflammation might account for preserved anti-incontinence effects of cut polypropylene sling in rat model.
abstract: OBJECTIVE
  To determine whether the inflammatory responses or collagen remodeling in suburethral tissue could have contributed to the preserved anti-incontinence effects of a cut polypropylene sling.
  
  
  METHODS
  Stress urinary incontinence was created in 60 age-matched female Sprague-Dawley rats that were subsequently randomized into 3 equal-size groups according to surgical procedure: placement of a vaginal suburethral sling, placement of a vaginal suburethral sling in which the suburethral portion of the sling was immediately cut, and sham surgery without placement of the sling. In a previous study, the leak point pressure measurements were obtained on these rats 6 weeks after surgery. The rats were then killed, the urethrovaginal tissue was harvested (cross-section of the entire urethra and anterior vagina) from 30 animals (10 from each experimental group), and the tissue was stained with hematoxylin-eosin and Masson's trichrome for histopathologic studies and picrosirius red for collagen fibers.
  
  
  RESULTS
  As previously published, the median leak point pressures were similarly and significantly increased in the rats in the intact and cut sling groups compared with those in the sham surgery group. The inflammatory mediators and interstitial edema were similarly increased in the intact and cut sling specimens compared with the sham surgery specimens. Under polarized light, picrosirius red-stained specimens from the sham surgery animals appeared to be composed of collagen that predominately birefringed red to yellow (typical of type I collagen). The picrosirius red-stained cut and intact sling specimens appeared to contain collagen fibers that predominately birefringed green (typical of type III collagen).
  
  
  CONCLUSIONS
  Histologic changes, including inflammation, localized edema, and differential collagen remodeling, might contribute to the preserved anti-incontinence mechanisms of cut or intact polypropylene slings observed clinically.
authors.affiliation: Department of Obstetrics and Gynecology, Cleveland Clinic, Cleveland, Ohio, USA.
authors.forename: Chi Chiung Grace
authors.initials: CC
authors.lastname: Chen
authors.forename: Adonis
authors.initials: A
authors.lastname: Hijaz
authors.forename: Judith A
authors.initials: JA
authors.lastname: Drazba
authors.forename: Margot S
authors.initials: MS
authors.lastname: Damaser
authors.forename: Firouz
authors.initials: F
authors.lastname: Daneshgari
mesh_terms: D000818:Animals; D003094:Collagen / Q000502:physiology*; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation; D011126:Polypropylenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D053825:Suburethral Slings; D016896:Treatment Outcome; D014521:Urethra / Q000502:physiology*; D014550:Urinary Incontinence, Stress / Q000601:surgery*
chemical_list: D011126:Polypropylenes; D003094:Collagen