Somatic 'soluble' adenylyl cyclase isoforms are unaffected in Sacy tm1Lex/Sacy tm1Lex 'knockout' mice.
BACKGROUND
Mammalian Soluble adenylyl cyclase (sAC, Adcy10, or Sacy) represents a source of the second messenger cAMP distinct from the widely studied, G protein-regulated transmembrane adenylyl cyclases. Genetic deletion of the second through fourth coding exons in Sacy(tm1Lex)/Sacy(tm1Lex) knockout mice results in a male sterile phenotype. The absence of any major somatic phenotype is inconsistent with the variety of somatic functions identified for sAC using pharmacological inhibitors and RNA interference.


RESULTS
We now use immunological and molecular biological methods to demonstrate that somatic tissues express a previously unknown isoform of sAC, which utilizes a unique start site, and which 'escapes' the design of the Sacy(tm1Lex) knockout allele.


CONCLUSIONS
These studies reveal increased complexity at the sAC locus, and they suggest that the known isoforms of sAC play a unique function in male germ cells.
Farrell
Jeanne
J
Department of Pharmacology, Weill Medical College of Cornell University, New York, New York, USA.
Ramos
Lavoisier
L
Tresguerres
Martin
M
Kamenetsky
Margarita
M
Levin
Lonny R
LR
Buck
Jochen
J
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D005821:Genetic Techniques; D007668:Kidney / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D010641:Phenotype; D020033:Protein Isoforms; D034622:RNA Interference
D017931:DNA Primers; D020033:Protein Isoforms; D000242:Cyclic AMP; D000262:Adenylyl Cyclases