Multiplexed screening of cellular uptake of gold nanoparticles using laser desorption/ionization mass spectrometry.
Gold nanoparticles (AuNPs) are highly promising candidates as drug delivery agents into cells of interest. We describe for the first time the multiplexed analysis of nanoparticle uptake by cells using mass spectrometry. We demonstrate that the cellular uptake of functionalized gold nanoparticles with cationic or neutral surface ligands can be readily determined using laser desorption/ionization mass spectrometry of cell lysates. The surface ligands have "mass barcodes" that allow different nanoparticles to be simultaneously identified and quantified at levels as low as 30 pmol. Using this method, we find that subtle changes to AuNP surface functionalities can lead to measurable changes in cellular uptake propensities.
Zhu
Zheng-Jiang
ZJ
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Ghosh
Partha S
PS
Miranda
Oscar R
OR
Vachet
Richard W
RW
Rotello
Vincent M
VM
D000818:Animals; D019556:COS Cells; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D016503:Drug Delivery Systems; D006046:Gold / Q000737:chemistry / Q000493:pharmacokinetics*; D008024:Ligands; D053768:Metal Nanoparticles / Q000032:analysis / Q000737:chemistry*; D008970:Molecular Weight; D010316:Particle Size; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
D008024:Ligands; D006046:Gold