title: Method and quantity of liquid formulation of phosphobacteria required for seed inoculation.
abstract: The survival of vegetative and sporulated cells of the Bacillus cultures on the seeds of the crop plants was tried in different combinations. One milliliter inoculum with 1 mL adhesive combination or sterile water showed better results followed by 1.5 mL inoculum with 0.5 mL adhesive or sterile water. The population of 5.5x10(5) cfu seed(-1) on black gram, 10.5x10(5) cfu seed(-1) on soybean and 6.5x10(5) cfu seed(-1) on maize were observed after 12 h of incubation in 1 mL sporulated inoculum mixed with 1 mL of rice gruel. The sporulated inoculum along with rice gruel favoured the adherence of the regenerated cells as rice gruel is rich in nutrient content.
authors.affiliation: Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Tamil Nadu, Coimbatore-641003, India.
authors.forename: M
authors.initials: M
authors.lastname: Gomathy
authors.forename: M
authors.initials: M
authors.lastname: Thangaraju
authors.forename: S
authors.initials: S
authors.lastname: Gunasekaran
authors.forename: N O
authors.initials: NO
authors.lastname: Gopal
authors.forename: H
authors.initials: H
authors.lastname: Gopal
mesh_terms: D001410:Bacillus megaterium / Q000302:isolation & purification*; D012639:Seeds / Q000382:microbiology*; D013025:Glycine max / Q000196:embryology