Phylogenetic relationships of Phytophthora andina, a new species from the highlands of Ecuador that is closely related to the Irish potato famine pathogen Phytophthora infestans.
Phylogenetic relationships of Phytophthora infestans sensu lato in the Andean highlands of South America were examined. Three clonal lineages (US-1, EC-1, EC-3) and one heterogeneous lineage (EC-2) were found in association with different host species in genus Solanum. The EC-2 lineage includes two mitochondrial (mtDNA) haplotypes, Ia and Ic. Isolates of P. infestans sensu lato EC-2 fit the morphological description of P. infestans but are different from any genotypes of P. infestans described to date. All isolates of P. infestans sensu lato from Ecuador were amplified by a P. infestans specific primer (PINF), and restriction fragment length patterns were identical in isolates amplified with ITS primers 4 and 5. The EC-1 clonal lineage of P. infestans sensu lato from S. andreanum, S. columbianum, S. paucijugum, S. phureja, S. regularifolium, S. tuberosum and S. tuquerense was confirmed to be P. infestans based on sequences of the cytochrome oxidase I (cox I) gene and intron 1 of ras gene. The EC-2 isolates with the Ic haplotype formed a distinct branch in the same clade with P. infestans and P. mirabilis, P. phaseoli and P. ipomoeae for both cox I and ras intron 1 phylogenies and were identified as the newly described species P. andina. Ras intron 1 sequence data suggests that P. andina might have arisen via hybridization between P. infestans and P. mirabilis.
Gómez-Alpizar
Luis
L
Agronomic Research Center, Box 2060, University of Costa Rica, San Pedro, Montes de Oca, Costa Rica.
Hu
Chia-Hui
CH
Oliva
Ricardo
R
Forbes
Gregory
G
Ristaino
Jean Beagle
JB
D004272:DNA, Mitochondrial / Q000235:genetics; D004484:Ecuador; D003576:Electron Transport Complex IV / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010838:Phytophthora / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D032322:Solanum / Q000382:microbiology*; D018631:ras Proteins / Q000235:genetics
D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D003576:Electron Transport Complex IV; D018631:ras Proteins