Aromatase (P450arom) and 11beta-hydroxylase (P45011beta) genes are differentially expressed during the sex change process of the protogynous rice field eel, Monopterus albus.
Steroids are known to play a crucial role in gonadal sex differentiation in many non-mammalian vertebrates, but also in the gonadal sex change of hermaphroditic teleosts. We investigated the expression of two genes encoding key steroidogenic enzymes, i.e., cytochrome P450 aromatase (P450arom) and cytochrome P45011beta-hydroxylase (P45011beta), during the sex change of the protogynous rice field eel, Monopterus albus. Using RT-PCR with degenerate primers, we cloned rice field eel homologous fragments for both genes (rcP450arom and rcP45011beta) as indicated by the high level of homology with P450arom and P45011beta sequences from various vertebrates. Gonadal expression of rcP450arom and rcP45011beta mRNA levels were then assessed during the sex change by semi-quantitative RT-PCR and a real-time RT-PCR. rcP450arom was predominantly expressed in ovary, much less in ovotestis, and barely in testis. Conversely, P45011beta was markedly up-regulated at the onset of testicular development. These findings underline that regulation of steroidogenesis is an important process in the sex change of protogynous rice field eel, and they clearly indicate that the concomitant down-regulation of P450arom and up-regulation of P45011beta are of pivotal importance to the sex change of this species.
Liu
Ji-Fang
JF
Key Laboratory of Protein Chemistry and Fish Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of China.
Guiguen
Yann
Y
Liu
Shao-Jun
SJ
D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012733:Sex Differentiation / Q000502:physiology*; D023701:Smegmamorpha / Q000378:metabolism*; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
D017931:DNA Primers; D001141:Aromatase; D013252:Steroid 11-beta-Hydroxylase