Stress response of Nidula niveo-tomentosa to UV-A light.
Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveotomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.
Taupp
Daniela E
DE
Zentrum Angewandte Chemie, Institut für Lebensmittelchemie der Leibniz Universitiit Hannover, Wunstorfer Strasse 14, D-30453 Hannover, Germany.
Nimtz
Manfred
M
Berger
Ralf G
RG
Zorn
Holger
H
D000363:Agaricales / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D018533:Biomass; D002074:Butanones / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D014466:Ultraviolet Rays
D002074:Butanones; D005656:Fungal Proteins; C035522:raspberry ketone