title: Structural analysis of point mutations in the Hairless gene and their association with the activity of the Hairless protein.
abstract: The Notch signaling pathway (NSP) is an important intercellular communication mechanism that regulates embryo development and adult physiological functions. The Hairless (H) protein is a powerful antagonist of the NSP by its interaction with the Suppressor of Hairless (Su[H]) protein, recruiting the corepressors Gro and CtBP. In the present work, we examined the role of several important amino acids in different H protein domains analyzing four mutant lines of Drosophila melanogaster. The mutant alleles were evaluated by single-strand conformational polymorphism (SSCP) analysis and we located mutated regions at different positions along the sequence of the Hairless gene.
authors.affiliation: Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Km. 9.5, Carretera Morelia-Zinapécuaro, C.P. 58893, La Palma, Tarímbaro, Michoacán, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Paz-Gómez
authors.forename: Víctor Manuel
authors.initials: VM
authors.lastname: Baizabal-Aguirre
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Valdez-Alarcón
authors.forename: Marcos
authors.initials: M
authors.lastname: Cajero-Juárez
authors.forename: Anja C
authors.initials: AC
authors.lastname: Nagel
authors.forename: Anette
authors.initials: A
authors.lastname: Preiss
authors.forename: Dieter
authors.initials: D
authors.lastname: Maier
authors.forename: Alejandro
authors.initials: A
authors.lastname: Bravo-Patiño
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000235:genetics* / Q000502:physiology; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D051880:Receptors, Notch / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; D014157:Transcription Factors; C076560:H protein, Drosophila