title: Histological and haematological disturbance caused by arsenic toxicity in mice model.
abstract: Histological and hematological disturbance caused by Arsenic containing water were studied in mice model. Animal were divided into four groups. Control group exposed to arsenic free distilled water and 3 treatmental group exposed to the arsenic containing water with 30, 150 and 300 ppb. Blood samples and organs were collected after 40 days. Histopathological results revealed mild to severe type of necrosis and degenerative changes in kidney and liver of arsenic feed animals. Kidney of the 300 ppb group showed severe type of necrosis and degenerative changes in distal and proximal tubules. The renocytes of proximal and distal tubules were showing hydropic and fatty degeneration. Due to degenerative changes cells were showing cytoplasmic vacoulation and cytoplasmic and nuclear blebbing. Glomeruli cells were contracted and increased the bowman's spaces. Varied degrees of changes were also observed in 30 and 150 ppb exposed group. Necrosis of hepatocytes and cytoplasmic blebbing were also observed. The sinusoidal spaces were expanded due to shrinkage and necrosis of hepatocytes. Spleenocytosis occurred in spleen and the parenchymal and mesenchymal cells were replaced by connective tissue. The lymphocytes were severely damaged by arsenic toxicity. White Blood Cells (WBCs), Red Blood Cells (RBCs) and hemoglobin level in control groups were in normal range where as level were significantly decreased with the increase dose of arsenic in the respective treatmental groups. The data was analyzed statistically and was found that significant was found among the group (p<0.05).
authors.affiliation: Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan.
authors.forename: Rubina
authors.initials: R
authors.lastname: Ferzand
authors.forename: Javaid Ali
authors.initials: JA
authors.lastname: Gadahi
authors.forename: Shamim
authors.initials: S
authors.lastname: Saleha
authors.forename: Qurban
authors.initials: Q
authors.lastname: Ali
mesh_terms: D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D001772:Blood Cell Count; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D051379:Mice; D013154:Spleen / Q000187:drug effects / Q000473:pathology
chemical_list: D001151:Arsenic