title: Probing osmotic effects on invertase with L-(-)-sucrose.
abstract: L-(-)-sucrose was efficiently synthesized using intramolecular aglycon delivery and used to elucidate osmotic effects on the activity of invertase, which catalyzes the hydrolysis of D-(+)-sucrose. The osmotic effect imposed by L-sucrose was responsible for more than 30% of the activity loss ascribed otherwise to "substrate inhibition."
authors.affiliation: Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907-2084, USA.
authors.forename: Seung-kee
authors.initials: SK
authors.lastname: Seo
authors.forename: Alexander
authors.initials: A
authors.lastname: Wei
mesh_terms: D008954:Models, Biological; D009995:Osmosis; D013379:Substrate Specificity; D013395:Sucrose / Q000737:chemistry*; D043324:beta-Fructofuranosidase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D013395:Sucrose; D043324:beta-Fructofuranosidase