title: Usefulness of rNcGRA7- and rNcSAG4-based ELISA tests for distinguishing primo-infection, recrudescence, and chronic bovine neosporosis.
abstract: Bovine reproductive failure caused by the parasite Neospora caninum is a major problem and is responsible for severe economic losses worldwide. Currently, appropriate control measures depend on the predominant transmission route in a particular herd. Therefore, the development of diagnostic tools capable of discriminating between primo-infection, recrudescence, re-infection, and chronic infection is a major challenge in the serodiagnosis of bovine neosporosis. Here, two recombinant protein-based ELISAs utilizing the immunodominant NcGRA7 dense granule protein and the NcSAG4 bradyzoite stage-specific protein were developed and showed good diagnostic performances. Their usefulness for discerning between primo-infection, recrudescence, re-infection, and chronic infection was also studied by analyzing an appropriate panel of serum samples belonging to different groups of experimentally and naturally infected bovines. Our results suggest that anti-rNcGRA7 antibody levels may be indicative of acute infection (primo-infection, re-infection, and recrudescence), whereas the presence of anti-rNcSAG4 antibodies may be associated with chronic infection and could be a good indicator of infection establishment (tachyzoite-bradyzoite conversion). Moreover, primo-infection associated with a Neospora-associated epidemic abortion pattern is characterized by the detection of anti-rNcGRA7 antibodies together with the absence or detection of anti-rNcSAG4 antibody levels around the cut-off point. In contrast, the detection of antibody levels directed against both recombinant proteins may be quite indicative of recrudescence or re-infection associated with abortion and/or vertical transmission in herds with a Neospora-associated endemic abortion pattern. In conclusion, both serological tests developed in the present study offer additional information to conventional avidity tests and, consequently, improve the diagnosis of bovine neosporosis with perspectives for control measures.
authors.affiliation: SALUVET, Animal Health Department, Complutense University of Madrid, Ciudad Universitaria s/n, 28040-Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Aguado-Martínez
authors.forename: G
authors.initials: G
authors.lastname: Alvarez-García
authors.forename: A
authors.initials: A
authors.lastname: Fernández-García
authors.forename: V
authors.initials: V
authors.lastname: Risco-Castillo
authors.forename: I
authors.initials: I
authors.lastname: Arnaiz-Seco
authors.forename: X
authors.initials: X
authors.lastname: Rebordosa-Trigueros
authors.forename: V
authors.initials: V
authors.lastname: Navarro-Lozano
authors.forename: L M
authors.initials: LM
authors.lastname: Ortega-Mora
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology; D002908:Chronic Disease; D003048:Coccidiosis / Q000175:diagnosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D008297:Male; D018688:Neospora; D012008:Recurrence