title: Functional implications of HNK-1 expression on invasive behaviour of melanoma cells.
abstract: HNK-1 epitope, also known as CD57, is expressed on a wide range of cells and has been related to cell to cell and cell to extracellular matrix interactions. Expression of the HNK-1 epitope has been considered a prognostic factor in several tumours, as it has been associated with the risk of metastasis. HNK-1 has been found to be expressed on uveal and cutaneous melanoma and proposed as a useful marker of the risk of metastasis. We have analysed the HNK-1 expression on a large panel of melanoma cell lines, the involvement of this epitope in melanoma cell adhesion, as well as its migrative and invasive behaviour. HNK-1 was highly expressed in 12.9% of melanoma cell lines, and in vitro experiments using invasive melanoma cell lines demonstrated that an HNK-1 blockade reduces cell adhesion to extracellular matrix as well as their migrative and invasive ability. These data support the functional relevance of HNK-1 expression in metastatic melanoma.
authors.affiliation: Department of Physiology, Immunology Unit, Faculty of Veterinary Science, University of Extremadura, Caceres, Spain. jgarcas@unex.es
authors.forename: Javier G
authors.initials: JG
authors.lastname: Casado
authors.forename: Elena
authors.initials: E
authors.lastname: Delgado
authors.forename: Evangelia
authors.initials: E
authors.lastname: Patsavoudi
authors.forename: Esther
authors.initials: E
authors.lastname: Durán
authors.forename: Beatriz
authors.initials: B
authors.lastname: Sanchez-Correa
authors.forename: Sara
authors.initials: S
authors.lastname: Morgado
authors.forename: Rafael
authors.initials: R
authors.lastname: Solana
authors.forename: Raquel
authors.initials: R
authors.lastname: Tarazona
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D018959:CD57 Antigens / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D010641:Phenotype; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D018959:CD57 Antigens