Localization and expression of a 70 kDa protein in goat spermatozoa having Na(+),K(+)-ATPase inhibitory and arylsulphatase A activities.
We have previously isolated and purified a goat sperm protein of 70 kDa molecular weight designated as P70 and characterized it as an inhibitor of Na(+),K(+)-ATPase. Our study reveals that the first 10 amino acid residues from the N-terminal end of P70 has high degree of homology with arylsulphatase A from mice, pig and human. Indirect immunofluorescence study shows the presence of the protein on goat sperm surface. Furthermore, live goat sperm and the extract of peripheral sperm plasma membrane proteins exhibit arylsulphatase A's desulphation activity. The P70 remains on the head surface of in vitro capacitated cauda epididymal sperm as shown by positive immunofluorescence staining of cauda sperm. Immunoblot and flow cytometric studies corroborate the above findings. The presence of P70 on capacitated cauda sperm surface suggest a possible role of this protein in sperm zona pellucida binding. In the present report we demonstrate arylsulphatase A like activity in P70 and describe its localization and expression in goat sperm.
Chatterjee
Madhumouli
M
Division of Molecular Medicine, Bose Institute, C.I.T. Scheme VII-M, Kolkata, India.
Das
Pradeep
P
Mazumder
Arindam
A
Nagdas
Subir Kumar
SK
Sen
Parimal Chandra
PC
D020101:Acrosome Reaction; D000818:Animals; D002553:Cerebroside-Sulfatase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism*; D004822:Epididymis / Q000166:cytology; D006041:Goats; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism
D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D002553:Cerebroside-Sulfatase; D000254:Sodium-Potassium-Exchanging ATPase