[Analysis of primary metabolites of alpha-tocopherol in human urine by liquid chromatography-mass spectrometry].
To investigate primary metabolites of alpha-tocopherol in human urine, the urine samples of five healthy volunteers after oral administration of 250 mg vitamin E once a day for seven days were collected within 0 -6 h in the seventh day. The samples were purified through C18 solid-phase extraction cartridge and analyzed by liquid chromatography-tandem mass spectrometry. alpha-Tocopheronic acid, 2,5,7, 8-tetramethyl-2-(2'-carboxyethyl) -6-suphate-chroman (alpha-CEHC-sulphate), gamma-tocopheronolactone, and 2, 5, 7, 8-tetramethyl-2-(4', 8', 12'-trimethyl-12'-carboxy dodecanyl) -6-suphate-chroman were found in urine of volunteers as four primary metabolites of alpha-tocopherol. The method has shown to be promising for alpha-tocopherol detection with many desirable properties including high sensitivity and selectivity, thus providing a reliable pathway for further study in metabolism of alpha-tocopherol.
Pan
Ying-Yu
YY
Department of Chemistry, South China Normal University, Guangzhou 510006, China.
Yu
Ying
Y
Lai
Gu-Xian
GX
Zhu
Li-Cai
LC
D000284:Administration, Oral; D000975:Antioxidants / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014810:Vitamin E / Q000493:pharmacokinetics*; D024502:alpha-Tocopherol / Q000378:metabolism* / Q000652:urine*
D000975:Antioxidants; D014810:Vitamin E; D024502:alpha-Tocopherol