title: Comparative analysis between Chamomilla recutita and corticosteroids on wound healing. An in vitro and in vivo study.
abstract: The comparison of chamomile and corticosteroids for treating ulcers was done in vitro and in vivo. The experimental groups were: control; chamomile recutita; triamcinolone acetonide and clobetasol propionate. For the in vitro study the cell viability of fibroblasts cultured for 24 h in media conditioned by the substances was obtained by the MTT reduction analysis. For the in vivo study, 125 male rats were submitted to experimental ulcers treated or not (control) by the substances tested. At 1, 3, 5, 7 and 14 days later 5 animals of each group were sacrificed. The lesions were analyzed by means of clinical observation and histological wound-healing grading. Data were compared by ANOVA (p <or= 0.05). All experimental groups presented positive cell viability in 24 h. The cultures treated with chamomile presented the smallest cell viability. All animals of the chamomile group exhibited complete wound healing 9 days before the other groups. Complete repaired lesions were observed after 5 days of treatment only in the chamomile group. Animals treated with chamomile presented significantly faster wound healing in comparison to those treated with corticosteroids. Based on the conditions of this study, we concluded that chamomile in comparison to corticosteroids promotes faster wound healing process.
authors.affiliation: Rehabilitation Science Post-Graduation Program, Nove de Julho University - UNINOVE, São Paulo - SP, Brazil. manomartins@gmail.com
authors.forename: Manoela Domingues
authors.initials: MD
authors.lastname: Martins
authors.forename: Márcia Martins
authors.initials: MM
authors.lastname: Marques
authors.forename: Sandra Kalil
authors.initials: SK
authors.lastname: Bussadori
authors.forename: Marco Antonio Trevizani
authors.initials: MA
authors.lastname: Martins
authors.forename: Vanessa Christina Santos
authors.initials: VC
authors.lastname: Pavesi
authors.forename: Raquel Agnelli
authors.initials: RA
authors.lastname: Mesquita-Ferrari
authors.forename: Kristianne Porta Santos
authors.initials: KP
authors.lastname: Fernandes
mesh_terms: D000305:Adrenal Cortex Hormones / Q000627:therapeutic use*; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002990:Clobetasol / Q000627:therapeutic use; D008297:Male; D031168:Matricaria / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D014222:Triamcinolone Acetonide / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000305:Adrenal Cortex Hormones; D002990:Clobetasol; D014222:Triamcinolone Acetonide