[Investigation of the mechanisms of crystallin aggregation induced by pulsed laser UV irradiation at 308 nm].
The results of the investigations of photoaggregation of the main eye lens proteins alpha-, beta- and gamma-crystallins and the model protein carbonic anhydrase in response to pulsed irradiation by a XeCI laser at 308 nm in the wide range of pulse energy densities (w) and pulse repetition rates (F) have been reviewed. A nonlinear dependence of aggregation efficiency on the values of w, F, and the concentration of protein solution was found. A theoretical model that qualitatively describes the experimental results was developed. The aggregation of N-amino-arm truncated beta A3-crystallin was analyzed. It was found that the loss of the N-amino-arm as a result of mutation or eye lens aging increases the probability of UV-induced beta-crystallin aggregation, thereby increasing the predisposition of eye lens to senile cataract. The influence of some short-chain peptides on the aggregation efficiency of beta-crystallin and beta-crystallin in solution with alpha-crystallin was investigated. Based on the results obtained, a combination of peptides (called "a new preparation") was found that most effectively delays the crystallin aggregation. The preparation has been probed on experimental animals. The trials showed that the preparation increases the delay in the development of UV-induced cataract in rats. The possibility of designing a drug for the prophylaxis of the development of cataract in humans based on this preparation is discussed.
Soustov
L V
LV
Chelnokov
E V
EV
Sapogova
N V
NV
Bitiurin
N M
NM
Nemov
V V
VV
Sergeev
Iu V
IuV
Ostrovskiĭ
M A
MA
D000818:Animals; D002386:Cataract / Q000209:etiology / Q000235:genetics / Q000517:prevention & control; D003459:Crystallins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007834:Lasers; D007908:Lens, Crystalline / Q000737:chemistry; D008956:Models, Chemical; D009154:Mutation; D010455:Peptides / Q000737:chemistry* / Q000627:therapeutic use; D051381:Rats; D014466:Ultraviolet Rays / Q000009:adverse effects
D003459:Crystallins; D010455:Peptides