High expression of Foxp3, IL-23p19 and survivin mRNA in colorectal carcinoma.
BACKGROUND
Cytokines have been suggested to both modulate anti-tumor responses and promote tumor growth.


METHODS
We analyzed the expression of pro-inflammatory IL-12p35, IL-12p40, IL-23p19, anti-inflammatory IL-10, antiapoptotic factor survivin, and transcription factors-RelA, c-Jun, and Foxp3 mRNA in patients' blood, colon carcinoma tissue, and in normal mucosal tissue by real-time polymerase chain reaction. The quantity determination of serum IL-12p40, IL-23, and IL-10 was performed by enzyme-linked immunosorbent assay.


RESULTS
We observed significantly higher levels in patients for all three analyzed cytokines, with IL-23 concentration change being the highest. We detected the greatest upregulation of IL-23p19, Foxp3 and survivin mRNA in colorectal carcinomas than normal mucosa. A statistically significant upregulation of IL-12p40, IL-10, and c-Jun mRNA but not for IL-12p35 and RelA mRNA in tumor tissue comparing to normal tissue was also established.


CONCLUSIONS
In conclusion, we show a characteristic gene expression profile combining markers associated with inhibition of anti-tumor immune response (Foxp3, IL-10), inhibition of apoptosis (survivin), and induction of the cytokines with protumoral activity as IL-12p40 and IL-23p19 (IL-23) in the colorectal tumor tissue but not in peripheral blood of patients.
Stanilov
Noyko
N
Department of Neurosurgery, Surgery and Urology, 2nd Surgery clinic, University Hospital, Faculty of Medicine, Trakia University, Stara Zagora, Bulgaria.
Miteva
Lyuba
L
Mintchev
Nikolay
N
Stanilova
Spaska
S
D015179:Colorectal Neoplasms / Q000097:blood / Q000235:genetics* / Q000601:surgery; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics; D053765:Interleukin-12 Subunit p35 / Q000235:genetics / Q000378:metabolism; D053762:Interleukin-12 Subunit p40 / Q000097:blood / Q000235:genetics; D053760:Interleukin-23 Subunit p19 / Q000097:blood / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D000077022:Survivin; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
C107489:BIRC5 protein, human; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D051034:Inhibitor of Apoptosis Proteins; D053765:Interleukin-12 Subunit p35; D053762:Interleukin-12 Subunit p40; D053760:Interleukin-23 Subunit p19; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D000077022:Survivin; D014157:Transcription Factors; D016753:Interleukin-10