title: A putative lipoprotein of Sphingomonas sp. strain A1 binds alginate rather than a lipid moiety.
abstract: Gram-negative Sphingomonas sp. strain A1 accumulates alginate in the cell surface pit and directly incorporates the polysaccharide into its cytoplasm through a 'superchannel'. A cell surface protein Algp7 (27 kDa) is inducibly expressed in the presence of alginate. Although the protein Algp7 was initially classified as a lipoprotein based on its primary structure, Algp7 purified from strain A1 cells did not possess a lipid moiety. Algp7 bound alginate efficiently at a neutral pH with a K(d) of 3.6 x 10(-8) M, suggesting that the cell surface protein contributed to accumulation of alginate in the pit.
authors.affiliation: Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan.
authors.forename: Jinshan
authors.initials: J
authors.lastname: He
authors.forename: Akihito
authors.initials: A
authors.lastname: Ochiai
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Fukuda
authors.forename: Wataru
authors.initials: W
authors.lastname: Hashimoto
authors.forename: Kousaku
authors.initials: K
authors.lastname: Murata
mesh_terms: D000464:Alginates / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D008074:Lipoproteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020578:Sphingomonas / Q000235:genetics / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D008074:Lipoproteins; D008565:Membrane Proteins; D020723:Glucuronic Acid