Biodegradable nanogels prepared by self-assembly of poly(L-lactide)-grafted dextran: entrapment and release of proteins.
We showed previously that poly(L-lactide)-grafted dextran could form biodegradable nanogels in water. In this paper, various properties of Dex-g-PLLA nanogels were compared with Dex-Chol (dextran-cholesterol conjugate) nanogels to investigate the effects of hydrophobic units. Dex-g-PLLA nanogels exhibited significantly lower CAC and higher colloidal stability, indicating a strong tendency to form nanogels. We prepared lysozyme-loaded Dex-g-PLLA nanogels, and they exhibited a sustained release of lysozyme for 1 week without denaturation in PBS at 37 degrees C. The Dex-g-PLLA nanogels therefore have great potential as a delivery vehicle for therapeutic protein.
Nagahama
Koji
K
Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, and High Technology Research Center, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
Ouchi
Tatsuro
T
Ohya
Yuichi
Y
D002784:Cholesterol; D003102:Colloids; D003911:Dextrans / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D009113:Muramidase / Q000008:administration & dosage / Q000493:pharmacokinetics; D049329:Nanostructures / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*
D003102:Colloids; D003911:Dextrans; D004337:Drug Carriers; D005782:Gels; D011091:Polyesters; C033616:poly(lactide); D002784:Cholesterol; D009113:Muramidase