[Establishment of a tight tetracycline-controlled HCV-C double transgenic mouse model].
OBJECTIVE
To develop a tight tetracycline-controlled HCV-C double transgenic mouse model.


METHODS
By crossbreeding of ApoE-rtTA-tTS transgenic mice with TRE-HCV-C transgenic mice, the double transgenic mice were produced in the F1 generation. The presence of HCV-C and tTS gene in the F1 generation was confirmed by PCR, followed by further identification and quantification of the transgene using Southern blot hybridization. The expression of HCV-C in the liver of the mouse model was detected immunohistochemically.


CONCLUSIONS
Two transgenic mice were obtained, which contained ApoE-rtTA-tTS and TRE-HCV-C genes in the genome. Five founders contained HCV-C gene as confirmed by PCR and Southern blot hybridization. The tight tetracycline-controlled system may facilitate further study of HCV-C gene expression and gene therapy of hepatic cellular carcinoma.
Shuai
Li-fang
LF
Center for Disease Control and Prevention, Guangzhou, China.
Tang
Bo-heng
BH
Zhang
Ruo-shuang
RS
Zhao
Yong
Y
Yang
Guo-zhu
GZ
Cheng
Xi-gu
XG
D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics*; D015139:Blotting, Southern; D001947:Breeding; D003433:Crosses, Genetic; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology; D018936:Hepatitis C Antigens / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction; D013752:Tetracycline / Q000494:pharmacology*; D015534:Trans-Activators / Q000235:genetics*; D014758:Viral Core Proteins / Q000235:genetics*
D001057:Apolipoproteins E; D018936:Hepatitis C Antigens; D015534:Trans-Activators; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus; D013752:Tetracycline