Rapid nanoparticle-mediated monitoring of bacterial metabolic activity and assessment of antimicrobial susceptibility in blood with magnetic relaxation.
Considering the increased incidence of bacterial infections and the emergence of multidrug resistant bacteria at the global level, we designed superparamagnetic iron oxide nanoparticles as nanosensors for the assessment of antimicrobial susceptibility through magnetic relaxation. In this report, we demonstrate that iron oxide nanosensors, either dextran-coated supplemented with Con A or silica-coated conjugated directly to Con A, can be used for the fast (1) quantification of polysaccharides, (2) assessment of metabolic activity and (3) determination of antimicrobial susceptibility in blood. The use of these polysaccharide nanosensors in the determination of antimicrobial susceptibility in the clinic or the field, and the utilization of these nanoprobes in pharmaceutical R&D are anticipated.
Kaittanis
Charalambos
C
Nanoscience Technology Center, University of Central Florida, Orlando, Florida, USA.
Nath
Sudip
S
Perez
J Manuel
JM
D000890:Anti-Infective Agents / Q000494:pharmacology*; D003208:Concanavalin A / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D018432:Drug Resistance, Multiple; D004867:Equipment Design; D005290:Ferric Compounds / Q000737:chemistry; D007700:Kinetics; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry; D053758:Nanoparticles; D011134:Polysaccharides / Q000737:chemistry; D011522:Protons; D012542:Scattering, Radiation; D012822:Silicon Dioxide / Q000737:chemistry
D000890:Anti-Infective Agents; D003911:Dextrans; D005290:Ferric Compounds; D011134:Polysaccharides; D011522:Protons; D003208:Concanavalin A; C000499:ferric oxide; D012822:Silicon Dioxide