title: High fractions of exogenous DNA in human buccal samples reduce the quality of large-scale genotyping.
abstract: Buccal cell samples are considered a reliable source of DNA for genotyping studies. However, a potential drawback is the presence of exogenous DNA that is coextracted with human genomic DNA. A set of saliva and cheek swab samples, in which the fraction of human DNA varies from 10 to 96%, was genotyped using the Affymetrix Mapping 500 K Array. Samples containing less than 30% human DNA performed poorly in terms of accuracy and reliability. Therefore, we recommend quantitating the amount of human DNA in buccal samples to be used for large-scale genotyping and eliminating samples with less than 30% human DNA.
authors.affiliation: Max Planck Institute for Evolutionary Anthropology, D-04103 Leipzig, Germany. david_lopez@eva.mpg.de
authors.forename: David López
authors.initials: DL
authors.lastname: Herráez
authors.forename: Mark
authors.initials: M
authors.lastname: Stoneking
mesh_terms: D016477:Artifacts; D002610:Cheek; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification*; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004247:DNA