[The study of dynamic acccumulation of camptothecin in Camptotheca acuminata fruit by TLCS].
OBJECTIVE
To establish TLC scanning method for the determination of camptothecin in Camptotheca acuminata fruit, and analysis the dynamic accumulation of camptothecin in Camptotheca acuminata fruit to find out the best collection period.


METHODS
Silica gel H-CMC-Na thin layer plate was adopted in the determination with chloroform-acetone (7 : 3) used as deeloper, Single-wavelength and linear scanning of TLC was used, and the detection wavelength was 360 nm.


RESULTS
There was a good linear relationship for Comptothecin within the range of 0.0542 - 0.3252 microg, the average recovery was 97.13%, RSD was 1.76%.


CONCLUSIONS
The method is accurate, simple and reliable, and can be used for the determination of camptothecin in Camptotheca acuminata fruit and dynamic accumulation research.
Jiang
Ming-Lian
ML
Nanning Institute for Food and Drug Control, Nanning 530001, China. jnl@nnifdc.com
Huang
Rui-Song
RS
Wu
Yi
Y
Zhou
Fang-Li
FL
Su
Qing
Q
Wei
Ai-Yan
AY
D029769:Camptotheca / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D002166:Camptothecin / Q000032:analysis* / Q000378:metabolism; D002855:Chromatography, Thin Layer / Q000379:methods*; D005638:Fruit / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D012621:Seasons; D013997:Time Factors
D002166:Camptothecin