Plasma cortisol changes and body composition in Stizostedion lucioperca exposed to handling stress.
Stizostedion lucioperca aquaculture/stoking, remains a restrained industry due to several factors such as the paucity of freshwater resources and studies on the physiological responses of this species under environmental changes. The fish were subjected to handling stress by holding them out of the water in a hand-held dip net for 30 sec and netting the fish from the rearing tanks and transferring them to a small confinement tank. Sufficient aeration was supplied to the confinement tank to revert additional stress from oxygen depletion. Then measured changes in plasma cortisol levels and the growth ability (body composition) in Stizostedion lucioperca subjected to handling stress. Blood samples were collected from the fish after exposure to the handling stress. Crude protein (Nx6.25) was determined according to the Kjeldahl method, moisture content was determined by oven drying at 105+/-2 degrees C to constant weight and ash by heating in a muffle furnace at 550 degrees C to constant weight. Total lipids were extracted according to the Bligh and Dyer method. The results indicated that, handling stress significantly increased the plasma levels of cortisol 59.04 ng mL(-1) versus 40.83 ng mL(-1) in control group. Also the decrease of the level of protein and lipid concentrations show a significant difference between treatment and control (p<0.05). As protein and lipid decreased, moisture increased from 78.19% in control to 80.40% in treatment groups. According to the results, there was no significant change in ash content in control and treatment groups which was about 9%. In other words, it could be emphasized that nutrition-related behavior of Stizostedion lucioperca resulting from the activation of the hypothalamic/inter-renal axis in response to stress despite of different reactions bear resemblance to that of other fishes. Present data indicate that cortisol appears to be adequate to assess stress in Stizostedion lucioperca.
Fatemeh
Abbasi
A
Department of Biology, University of Alzahra, Tehran, Iran.
Sanaz
Ghafori
G
Shahla
Jamili
J
D000818:Animals; D001823:Body Composition; D006234:Handling, Psychological; D006854:Hydrocortisone / Q000097:blood*; D010471:Perches / Q000033:anatomy & histology / Q000097:blood / Q000502:physiology*; D012149:Restraint, Physical; D013312:Stress, Physiological
D006854:Hydrocortisone