Multilocus genotyping and molecular phylogenetics resolve a novel head blight pathogen within the Fusarium graminearum species complex from Ethiopia.
A survey of Fusarium head blight (FHB)-contaminated wheat in Ethiopia recovered 31 isolates resembling members of the Fusarium graminearum species complex. Results of a multilocus genotyping (MLGT) assay for FHB species and trichothecene chemotype determination suggested that 22 of these isolates might represent a new species within the Fg complex. Phylogenetic analyses of multilocus DNA sequence data resolved the 22 Ethiopian isolates as a novel, phylogenetically distinct species. The new species also appears to be novel in that MLGT probe data and sequence analysis of both ends of the TRI-cluster identified 15ADON and NIV recombination blocks, documenting inter-chemotype recombination involving the chemotype-determining genes near the ends of the TRI-cluster. Results of pathogenicity experiments and analyses of trichothecene mycotoxins demonstrated that this novel Fg complex species could induce FHB on wheat and elaborate 15ADON in planta. Herein the FHB pathogen from Ethiopia is formally described as a novel species.
O'Donnell
Kerry
K
Microbial Genomics Research Unit, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, 1815 N. University St., Peoria, IL 61604, USA. kerry.odonnell@ars.usda.gov
Ward
Todd J
TJ
Aberra
Dereje
D
Kistler
H Corby
HC
Aoki
Takayuki
T
Orwig
Nathane
N
Kimura
Makoto
M
Bjørnstad
Smund
S
Klemsdal
Sonja S
SS
D004271:DNA, Fungal / Q000235:genetics; D005002:Ethiopia; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D005838:Genotype; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D009183:Mycotoxins / Q000378:metabolism; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D014908:Triticum / Q000382:microbiology*
D004271:DNA, Fungal; D005656:Fungal Proteins; D009183:Mycotoxins