The late-stage foetal liver microenvironment is essential for later sensitivity of B-lymphopoiesis to suppression by oestrogens.
B-lymphopoiesis in FL differs notably from that of adult B-lymphopoiesis in being resistant to suppression by oestrogens due to the lack of expression of oestrogen receptors in B-cell progenitors and precursors. We have transplanted middle-stage FL cells (E14.5) to adult male mice and demonstrated that B-lymphopoiesis derived from FL cells remained resistant to suppression by exogenous oestrogen for several months compared to adult BM cells. This significant difference strongly suggests that the latestage FL environment exerts an inductive action on the haematopoietic stem cells and is mandatory for later sensitivity of B-lymphopoiesis to suppression by oestrogens. The results also provide the first in vivo functional confirmation of a differential responsiveness of FL- and adult BM-derived B-lymphopoiesis to suppression by oestrogens.
Pelichovská
T
T
Charles University in Prague, First Faculty of Medicine, Institute of Pathophysiology, Centre of Experimental Haematology, Prague, Czech Republic.
Chang
K T
KT
Sefc
L
L
Savvulidi
F
F
Broulík
P
P
Necas
E
E
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D004777:Environment; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005333:Fetus / Q000033:anatomy & histology / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000502:physiology*; D038041:Lymphopoiesis / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D011828:Radiation Chimera; D033581:Stem Cell Transplantation
D004967:Estrogens