title: Local effects of TL01 phototherapy in psoriasis.
abstract: The mechanisms whereby narrowband ultraviolet B (UVB) (311-313 nm, TL01) phototherapy are effective in psoriasis may differ from those occurring in broadband UVB phototherapy. In the present study, changes in epidermal cells as a result of TL01 therapy were assessed in the skin of patients with psoriasis. The non-lesional skin of five subjects with plaque psoriasis was biopsied before and after a series of 12 whole-body TL01 treatments. Following appropriate staining of skin sections, the numbers of p53-positive keratinocytes, sunburn cells and Langerhans cells in the epidermis were counted. TL01 therapy induced a threefold increase in the number of p53-positive epidermal cells, a 12-fold increase in sunburn cells and a twofold decrease in Langerhans cells. The increase in epidermal p53 expression and apoptosis of keratinocytes together with the depletion of Langerhans cells in the non-lesional skin of psoriasis patients are likely to contribute to the effectiveness of TL01 phototherapy.
authors.affiliation: Department of Dermatology, University of Edinburgh, Edinburgh, UK.
authors.forename: Bernadette
authors.initials: B
authors.lastname: DeSilva
authors.forename: Roddie C
authors.initials: RC
authors.lastname: McKenzie
authors.forename: John A A
authors.initials: JA
authors.lastname: Hunter
authors.forename: Mary
authors.initials: M
authors.lastname: Norval
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000528:radiation effects*; D002452:Cell Count; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000473:pathology; D007801:Langerhans Cells / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014467:Ultraviolet Therapy / Q000379:methods
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53