title: Flavobacterium sp. strain 4221 and Pedobacter sp. strain 4236 beta-1,3-glucanases that are active at low temperatures.
abstract: Secretion of beta-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of beta-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hydrolyzed fungal cell walls and retained activity at low temperatures.
authors.affiliation: Bioneer A/S, Kogle Allé 2, DK-2970 Hørsholm, Denmark.
authors.forename: Mikkel A
authors.initials: MA
authors.lastname: Rasmussen
authors.forename: Søren M
authors.initials: SM
authors.lastname: Madsen
authors.forename: Peter
authors.initials: P
authors.lastname: Stougaard
authors.forename: Mads G
authors.initials: MG
authors.lastname: Johnsen
mesh_terms: D000595:Amino Acid Sequence; D041963:Bacteroidetes / Q000201:enzymology*; D001483:Base Sequence; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D003080:Cold Temperature; D004926:Escherichia coli / Q000235:genetics; D005417:Flavobacterium / Q000201:enzymology*; D015870:Gene Expression; D043326:Glucan 1,3-beta-Glucosidase / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics; D008969:Molecular Sequence Data; D013696:Temperature
chemical_list: D043326:Glucan 1,3-beta-Glucosidase