title: An immunocytochemical, histochemical and ultrastructural study of the nervous system of the tapeworm Cyathocephalus truncatus (Cestoda, Spathebothriidea).
abstract: This study is the first detailed study of the organisation of the neuromuscular system of Cyathocephalus truncatus (Cestoda, Spathebothriidea). Five techniques have been used: (1) immunocytochemistry, (2) staining with TRITC-conjugated phalloidin, (3) NADPHdiaphorase histochemistry, (4) confocal scanning laser microscopy and (5) transmission electron microscopy. The patterns of nerves immunoreactive (IR) to antibodies towards serotonin (5-HT) and the invertebrate neuropeptide FMRFamide are described in relation to the musculature. The patterns of NADPHdiaphorase positive nerves and 5-HT-IR nerves are compared. The fine structure of the nervous system (NS) is described. The organisation of NS in the non-segmented, polyzoic C. truncatus differs clearly from that in the non-segmented, monozoic Caryophyllaeus laticeps and shows distinct similarities with the NS in pseudophyllidean cestodes. This supports the hypothesis that taxon Caryophyllidea and Spatheobothriidea form independent lineages within Eucestoda.
authors.affiliation: Centre of Parasitology of A. N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Lenin Avenue 33, 119071 Moscow, Russia.
authors.forename: Nadezhda B
authors.initials: NB
authors.lastname: Terenina
authors.forename: Larisa G
authors.initials: LG
authors.lastname: Poddubnaya
authors.forename: Oleg O
authors.initials: OO
authors.lastname: Tolstenkov
authors.forename: Margaretha K S
authors.initials: MK
authors.lastname: Gustafsson
mesh_terms: D000818:Animals; D002589:Cestoda / Q000648:ultrastructure*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D009132:Muscles / Q000648:ultrastructure*; D009252:NADPH Dehydrogenase / Q000032:analysis; D009420:Nervous System / Q000648:ultrastructure*
chemical_list: D009252:NADPH Dehydrogenase