Solid surface chemical and physical effects on the adsorption of recombinant Factor VIII.
The adsorption of a recombinant Factor VIII to silica surfaces coated to present different hydrophilic/hydrophobic, electrostatic and steric characteristics was monitored in situ, with ellipsometry. rFVIII adsorption affinity was high for hydrophobic surfaces and hydrophilic, charged surfaces. A lower affinity was recorded between rFVIII and a neutral hydrophilic surface, with substantial reduction in rFVIII adsorption to electronically neutral surfaces presenting pendant poly[ethylene oxide] chains. We conclude that steric repulsion is a requirement for minimizing rFVIII adsorption at solid surfaces.
Tai
Yuan-Ching
YC
Department of Chemical Engineering, Oregon State University, Corvallis, OR 97331, USA.
McGuire
Joseph
J
Joshi
Omkar
O
Wang
D Q
DQ
D000327:Adsorption; D055598:Chemical Phenomena; D005169:Factor VIII / Q000737:chemistry* / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D011092:Polyethylene Glycols / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D055672:Static Electricity; D013237:Stereoisomerism; D013499:Surface Properties
D011092:Polyethylene Glycols; D012822:Silicon Dioxide; C078147:F8 protein, human; D005169:Factor VIII