Extractive spectrophotometric determination of TRODAT-1 hydrochloride in lyophilized kit.
A simple, sensitive, and accurate spectrophotometric method has been developed for the assay of TRODAT-1 hydrochloride in lyophilized kit. The method is based on the formation of ion-pair association complex of TRODAT-1 with bromothymol blue (BTB) in disodium hydrogen phosphate/citric acid buffer of pH 4.0. The colored product was extracted with chloroform, and measured spectrophotometrically at 414 nm. Beer's law was obeyed in the range of 5-25 microg/ml with molar absorptivity of 2.75 x 10(4) l/mol/cm. Optimization of experimental conditions was described for the method. The proposed method has been successfully applied for the analysis of TRODAT-1 hydrochloride in lyophilized kit. No interference with pharmaceutical excipients was observed.
Li
X M
XM
Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, P.R. China.
Chen
Z P
ZP
Wang
S P
SP
Tang
J
J
Liu
C Y
CY
Zou
M F
MF
D001979:Bromthymol Blue; D004396:Coloring Agents; D005612:Freeze Drying; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015609:Organotechnetium Compounds / Q000032:analysis*; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet; D014326:Tropanes / Q000032:analysis*
D004396:Coloring Agents; D007202:Indicators and Reagents; D015609:Organotechnetium Compounds; D014326:Tropanes; C104196:technetium Tc 99m TRODAT-1; D001979:Bromthymol Blue