Antioxidative and anti-inflammatory protection of oleanolic acid and ursolic acid in PC12 cells.
PC12 cells were used to examine the in vitro antioxidative and anti-inflammatory effects of oleanolic acid (OA) and ursolic acid (UA). PC12 cells were pretreated with OA or UA at 20 and 40 microM and followed by exposure of hydrogen peroxide (H(2)O(2)) or 1-methyl-4-phenylpyridinium ion (MPP(+)) to induce cell injury. Results showed that H(2)O(2)- or MPP(+)-treatment significantly decreased cell viability and increased lactate dehydrogenase (LDH) release (P < 0.05). The pretreatment from OA or UA significantly and concentration-dependently reduced subsequent H(2)O(2)- or MPP(+)-induced cell death and LDH release (P < 0.05). Either H(2)O(2)- or MPP(+)-treatment significantly increased malonyldialdehyde (MDA) formation, decreased glutathione (GSH) content, and diminished glutathione peroxidase (GPX), catalase, and superoxide dismutase (SOD) activities (P < 0.05). The pretreatment from OA or UA significantly retained GSH, and reversed H(2)O(2)- and MPP(+)-induced impairment in catalase and SOD activities (P < 0.05), and decreased MDA formation (P < 0.05). Either H(2)O(2)- or MPP(+)-treatment significantly elevated interleukin-6 (IL-6) and tumor necrosis factor (TNF)-alpha levels (P < 0.05). The pretreatments from OA or UA significantly attenuated subsequent H(2)O(2)- or MPP(+)-induced release of IL-6 and TNF-alpha (P < 0.05). Based on the observed antioxidative and anti-inflammatory activities from OA and UA, these 2 compounds were potent agents against neurodegenerative disorder.
Tsai
Shih-Jei
SJ
Dept of Neurology, Chung Shan Medical Univ Hospital, Taiwan.
Yin
M-C
MC
D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D009828:Oleanolic Acid / Q000494:pharmacology*; D018384:Oxidative Stress; D016716:PC12 Cells; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000097245:Ursolic Acid
D015655:1-Methyl-4-phenylpyridinium; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D006861:Hydrogen Peroxide; D015850:Interleukin-6; D007770:L-Lactate Dehydrogenase; D008315:Malondialdehyde; D009828:Oleanolic Acid; D013482:Superoxide Dismutase; D014315:Triterpenes; D014409:Tumor Necrosis Factor-alpha; D000097245:Ursolic Acid