title: Application of sandwich ELISA for detecting tag fusion proteins in high throughput.
abstract: Based on a series of mAbs against four frequently used tags--the human Ig Fc fragment, GST, maltose-binding protein, and thioredoxin--we developed corresponding sandwich enzyme-linked immunosorbent assay (ELISA) to detect these tag fusion proteins. As a supplement for Western blot, the successfully established ELISA was specific, sensitive, quantitative, easy to perform, time-saving, and last but not least, suitable for high-throughput screening of tag fusion proteins. Determination of soluble tag fusion proteins expressed by various systems with the sandwich ELISA developed in the present study could be a valuable and promising tool for the wide application of tag-protein fusion systems in the rapidly growing field of proteomics research.
authors.affiliation: Department of Immunology, The Fourth Military Medical University, 17 ChangleWest Road, Xi'an, 710032, Shannxi, China.
authors.forename: Zhu-wei
authors.initials: ZW
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Chao-jun
authors.initials: CJ
authors.lastname: Song
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Ran
authors.initials: R
authors.lastname: Zhuang
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: An-gang
authors.initials: AG
authors.lastname: Yang
authors.forename: Bo-quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins