[Experimental study of transplanting human mesenchymal stem cells via portal vein to treat acute liver injury of mice induced with acetaminophen].
OBJECTIVE
To evaluate the effects of human mesenchymal stem cells (hMSCs) transplantation via portal vein to treat acute liver injury in mice induced with acetaminophen.


METHODS
A model of acute liver injury was established by acetaminophen gavage with a dose of 500 mg/kg. Twenty severe combined immune deficient mice (SCID mice) were randomly divided into 2 groups; one with hMSCs transplantation via their portal veins, the other group served as controls and only saline was infused into their veins. Liver function tests, fluorescein staining and reticular fiber staining of liver histological preparations and fluorescence- and light-microscopy were applied to observe the biochemical and pathological changes in the mice before and after the transplantation of hMSCs.


RESULTS
Liver function of the hMSCs group was significantly better than that of the controls (P less than 0.05). Fluorescence microscopy revealed that the hMSCs appeared in the areas of the periportal veins at first and then extended to the central vein areas; the reticular fiber staining indicated that hMSCs could repair the architecture of the hepatic acini. No prominent fibrosis and pseudolobules were found.


CONCLUSIONS
hMSCs transplantation via portal vein to SCID mice with acute liver injury induced by acetaminophen can improve their liver function effectively; hMSCs growth in their livers and acinus reconstruction can be affected. We think it is a good method to treat acute liver injury.
Liu
Ying-di
YD
Department of Gastroenterology, Chinese PLA General Hospital, Beijing 100853, China. liuyingdi301@sina.com
Yang
Yun-sheng
YS
Wang
Yun-fang
YF
Nan
Xue
X
Peng
Li-Hua
LH
Sun
Gang
G
Li
Ming-yang
MY
Pei
Xue-tao
XT
D000082:Acetaminophen / Q000009:adverse effects; D000818:Animals; D001854:Bone Marrow Cells; D056486:Chemical and Drug Induced Liver Injury / Q000601:surgery*; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051379:Mice; D016513:Mice, SCID; D011169:Portal Vein / Q000601:surgery
D000082:Acetaminophen