Distinct fractional Abeta release patterns in human mononuclear phagocytes.
The presence of cells of the mononuclear phagocyte lineage surrounding neuritic and vascular beta-amyloid (Abeta) plaques is a hallmark of Alzheimer's disease (AD) neuropathology. The fractional Abeta peptide patterns released by human mononuclear phagocyte (MNP), lymphocyte and PBMC cultures were assessed by Abeta-SDS-PAGE/immunoblot and compared with the Abeta patterns in neuronal supernatants, in cerebrospinal fluid (CSF), and plasma. MNP released substantial amounts of Abeta peptides and the Abeta pattern contrasted with that in neuronal supernatants, CSF and plasma by reduced Abeta(1-42) and increased proportions of N-truncated Abeta species. MNP derived from early AD patients released significantly more total Abeta peptides than age-matched controls. The proportion of Abeta(1-42) was not altered.
Maler
Juan Manuel
JM
Department of Psychiatry and Psychotherapy, University of Erlangen-Nuremberg, Schwabachanlage 6, D-91054 Erlangen, Germany. jmmaler@jm-m.de
Spitzer
Philipp
P
Klafki
Hans-Wolfgang
HW
Esselmann
Hermann
H
Lewczuk
Piotr
P
Kornhuber
Johannes
J
Herrmann
Martin
M
Wiltfang
Jens
J
D000328:Adult; D000368:Aged; D000544:Alzheimer Disease / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D016022:Case-Control Studies; D002478:Cells, Cultured; D002642:Chick Embryo; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012157:Mononuclear Phagocyte System / Q000378:metabolism* / Q000473:pathology*; D009474:Neurons / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D018950:Lipopolysaccharide Receptors; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40)