Functional expression of the glycine transporter 1 on bullfrog retinal cones.
Using patch clamp techniques, we characterized glycine-induced currents from cones in bullfrog retinal slices. Application of glycine to cone terminals induced an inward current, which was in part suppressed by strychnine. The remaining strychnine-resistant current component, which did not show polarity reversion in a range of -120 mV to +40 mV, was blocked by N[3-(4'-fluorophenyl)-3-(4'-phenylphenoxy)propyl] sarcosine, an antagonist of glycine transporter 1 (GlyT1), but not affected by amoxapine, an inhibitor of glycine transporter 2. Application of sarcosine, an agonist of GlyT1, to cone terminals induced an inward current that was completely suppressed by N[3-(4'-fluorophenyl)-3-(4'-phenylphenoxy)propyl] sarcosine or when external Na in Ringer's was replaced by choline. All these results show for the first time the functional expression of GlyT1 on bullfrog cones.
Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China.
Xin-Jun
XJ
Zhang
Li-Hao
LH
Ge
Jian
J
Liu
Xiong-Li
XL
Yang
D000657:Amoxapine / Q000494:pharmacology; D000818:Animals; D005998:Glycine / Q000494:pharmacology; D018684:Glycine Agents / Q000494:pharmacology; D050487:Glycine Plasma Membrane Transport Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011892:Rana catesbeiana; D017949:Retinal Cone Photoreceptor Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012521:Sarcosine / Q000494:pharmacology; D013331:Strychnine / Q000494:pharmacology
D018684:Glycine Agents; D050487:Glycine Plasma Membrane Transport Proteins; D014179:Neurotransmitter Uptake Inhibitors; D013331:Strychnine; D000657:Amoxapine; D005998:Glycine; D012521:Sarcosine