Comparative study of genes expressed from rice fungus-resistant and susceptible lines during interactions with Magnaporthe oryzae.
Rice blast disease caused by Magnaporthe oryzae is the most important fungal disease of rice. To understand the molecular basis of interaction between the fungus and rice, we constructed a cDNA library from a rice-resistant line inoculated with M. oryzae. One hundred and fifty-three cDNA clones were sequence analyzed, of which 129 exhibited significant nucleotide sequence homology to known genes, 21 were homologous to unknown genes, while three clones did not match to any database. However, these three unmatched clones showed sequence homology at protein level in the protein databases and one of them encoded a disease resistance-related protein kinase and was abundant in the EST collection. Northern analysis showed that this disease resistance-related protein kinase gene was induced by inoculation and only expressed in the rice-resistant, but not susceptible, lines. Southern analysis showed that this gene was present in a single copy in the rice genome and co-segregated with the M. oryzae resistance in the cross of the resistant and susceptible lines. This study illustrates that sequencing of ESTs from inoculated resistant plants can reveal genes responsive to pathogen infection, which could help understand plant defense mechanisms.
Australian Centre for Plant Functional Genomics, the University of Adelaide, Urrbrae, SA 5064, Australia. bujun.shi@adelaide.edu.au <bujun.shi@adelaide.edu.au>
Bu-Jun
BJ
Shi
Guo-Liang
GL
Wang
D015152:Blotting, Northern; D015139:Blotting, Southern; D002118:Calcium / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D020022:Genetic Predisposition to Disease; D007113:Immunity, Innate; D020082:Magnaporthe / Q000235:genetics* / Q000378:metabolism*; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology
D018076:DNA, Complementary; D002118:Calcium