Supercritical fluid chromatography comparison of the poly(trans-1,2-cyclohexanediyl-bis acrylamide) (P-CAP) column with several derivatized polysaccharide-based stationary phases.
The poly(trans-1,2-cyclohexanediyl-bis acrylamide) (P-CAP) column has so far been primarily used with normal phase and polar organic mobile phase chromatography. Its use in supercritical fluid chromatography (SFC) was investigated via the analysis of 40 commercial and 100 proprietary compounds using a 12-min gradient with methanol as a modifier. Results were then compared against those obtained from the popular derivatized polysaccharide-based chiral stationary phases (CSPs) such as Chiralpak AD-H and Chiralpak AS-H as well as Chiralcel OD-H and Chiralcel OJ-H columns. P-CAP demonstrated separation of 25% of the 140 total compounds, while each of the derivatized polysaccharide-based CSPs separated at least 46%. A study that compared the loading of 1,1'-bi-2-naphthol with P-CAP and Chiralpak AS columns indicated a similar trend in resolution vs. amount injected, though AS appeared capable of allowing a greater loading of material. The P-CAP column was found to be beneficial in the separation of a complex mixture of enantiomers and achiral impurities, where the derivatized polysaccharide-based columns did not show as desirable of a separation. A key advantage of this type of chiral stationary phase is the fact that it is available in both enantiomeric forms, allowing manipulation of elution order of enantiomers, which is especially helpful for preparative applications. P-CAP also demonstrated that it could resolve an achiral impurity from the desired compound in a different mixture, while the same impurity co-eluted on the Chiralpak AD-H column. Overall, the synthetic polymer-based P-CAP showed less chiral discrimination power compared to the derivatized polysaccharide-based CSPs under the conditions explored in this study.
Barnhart
Wesley W
WW
Discovery Analytical Sciences, Molecular Structure, Chemistry Research and Discovery, Amgen Inc., One Amgen Center Dr, Thousand Oaks, CA 91320, USA.
Gahm
Kyung H
KH
Hua
Zheng
Z
Goetzinger
Wolfgang
W
D000178:Acrylamides / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D003510:Cyclohexanes / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry*; D013237:Stereoisomerism
D000178:Acrylamides; D003510:Cyclohexanes; D004364:Pharmaceutical Preparations; D011134:Polysaccharides; C502589:poly (trans-1,2-cyclohexanediyl-bis acrylamide)