[Actinobacterial diversity of a sediment sample from Xiaoerkule Lake].
OBJECTIVE
The aim of study was to investigate Actinobacterial diversity in Xiaoerkule salt lake, to lay a foundation for furthering to tap.


METHODS
Actinobacterial diversity in this sediment from Xiaoerkule Lake was investigated by culture-independent method and phylogenetic analysis based on 16S rRNA gene sequences. Total DNA of sediment sample was extracted using SDS-CTAB method. The primers for the class Actinobacteria were used for actinobacterial 16S rRNA gene amplification and then a clone library was constructed for the sediment sample.


RESULTS
Fifty-one clones screened from 160 clones on the basis of Hae III digestion patterns were sequenced, and their sequences were deposited in the GenBank. Clone sequences (52.9%) belonged to Acidimicrobidae and 5 suborders of Actinobacteridae. The other clone sequences (47.1%), which formed one large distinct clade in phylogenetic tree among phylum Actinobacteria, may represent one new suborder or new class.


CONCLUSIONS
There was abundant actinobacterial diversity in the sediment of Xiaoerkule Lake, and the result implied that there were large numbers of unknown actionobacterial groups here.
Key Laboratory of Protection and Utilzation of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, China. guantongweily@163.com
Tongwei
T
Guan
Jinyuan
J
Wu
Xiaoyang
X
Zhi
Shukun
S
Tang
Lihua
L
Xu
Wenjun
W
Li
Lili
L
Zhang
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D002681:China; D003247:Conservation of Natural Resources; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S