Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA.
dATP derivatives bearing Br, Me or Ph groups in position 8 were prepared and tested as substrates for DNA polymerases to show that 8-Br-dATP and 8-Me-dATP were efficiently incorporated, while 8-Ph-dATP was a poor substrate due to its bulky Ph group.
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead & IOCB Research Center, Flemingovo nam. 2, CZ-16610, Prague 6, Czech Republic.
Hana
H
Cahová
Radek
R
Pohl
Lucie
L
Bednárová
Katerina
K
Nováková
Josef
J
Cvacka
Michal
M
Hocek
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D044366:Transition Temperature
D003838:Deoxyadenine Nucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase; C026600:2'-deoxyadenosine triphosphate