[Studies on chemical constituents from Euonymus alatus II].
OBJECTIVE
To study the chemical constituents and antitumor activities of Euonymus alatus.


METHODS
Compounds were isolated and purified repeatedly by silica gel, Sephadex LH-20 column chromatography, and their chemical structures were elucidated by their physicochemical properties and spectral data, such as 1H-NMR, 13C-NMR and EI-MS. The trypan blue experiments in vitro were used to observe the antitumor activity of several compounds.


RESULTS
Ten compounds were obtained and identified as (+)-usnic acid (1), benzoic acid (2), 2-hydroxy-4-methoxy-3, 6-dimethyl benzoic acid (3), lupeol (4), 3 beta-hydroxy-30-norlupan-20-one (5), (2R, 3R)-3, 5, 7, 4'-tetrahydroxy flavanone (6), kaempferol (7), 5, 7, 4'-trihydroxy flavanone (8), quercetin (9) and daucosterol (10).


CONCLUSIONS
Compounds 1, 2, 5 and 8 were isolated from this genus for the first time. The trypan blue experiments in vitro showed that compounds 1, 4 and 5 had growth inhibitory effects on HL-60 cells.
Fang
Zhen-feng
ZF
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Li
Zhan-lin
ZL
Wang
Yu
Y
Hua
Hui-ming
HM
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D029756:Euonymus / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans
D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal