Blood culture collection technique and pneumococcal surveillance in Malawi during the four year period 2003-2006: an observational study.
BACKGROUND
Blood culture surveillance will be used for assessing the public health effectiveness of pneumococcal conjugate vaccines in Africa. Between 2003 and 2006 we assessed blood culture outcome and performance in adult patients in the central public hospital in Blantyre, Malawi, before and after the introduction of a dedicated nurse led blood culture team.


METHODS
A prospective observational study.


RESULTS
Following the introduction of a specialised blood culture team in 2005, the proportion of contaminated cultures decreased (19.6% in 2003 to 5.0% in 2006), blood volume cultured increased and pneumococcal recovery increased significantly from 2.8% of all blood cultures to 6.1%. With each extra 1 ml of blood cultured the odds of recovering a pneumococcus increased by 18%.


CONCLUSIONS
Standardisation and assessment of blood culture performance (blood volume and contamination rate) should be incorporated into pneumococcal disease surveillance activities where routine blood culture practice is constrained by limited resources.
Mtunthama
Neema
N
Malawi-Liverpool-Liverpool Wellcome Trust Laboratories, Blantyre, Malawi. nmtunthama@mlw.medcol.mw
Gordon
Stephen B
SB
Kusimbwe
Temwa
T
Zijlstra
Eduard E
EE
Molyneux
Malcolm E
ME
French
Neil
N
D000328:Adult; D001431:Bacteriological Techniques / Q000379:methods / Q000592:standards*; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards*; D006779:Hospitals, Public / Q000458:organization & administration* / Q000592:standards; D006801:Humans; D008295:Malawi / Q000453:epidemiology; D008485:Medical Audit; D009726:Nurses; D011008:Pneumococcal Infections / Q000453:epidemiology; D011159:Population Surveillance; D011446:Prospective Studies; D013296:Streptococcus pneumoniae / Q000302:isolation & purification*