Suppressive effects of cadmium on neurons and affected proteins in cultured developing cortical cells.
The purpose of the present study was to examine the in vitro effects of low-dose cadmium (Cd) on developing cortical cells. The cortical cells removed from fetuses (embryonic day 15) were treated with 10nM of Cd for 24h. The effects of Cd on dendritic and synaptic development were immunocytochemically observed with anti-microtubule associated protein-2 (MAP2) and anti-synapsin I antibodies, respectively. Administration of Cd suppressed dendritic as well as synaptic development at 10nM. By two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectrometric (LC/MS/MS) analysis, we identified three proteins with different expression after Cd-treatment; dihydropyrimidinase-related protein 2 (DRP-2/CRMP-2), 14-3-3-epsillon and calmodulin (CaM). Though the number of identified proteins was small, these proteins are known to be involved in neuronal development. The present study demonstrated the morphological effects as well as affected proteins in Cd-treated cortical cells, providing tools and insights in elucidating mechanisms how low-dose Cd distorts brain development.
Department of Life Sciences, Toyo University, 1-1-1 Itakura, Oura, Gunma 374-0193, Japan. r-kaneko@toyonet.toyo.ac.jp
Ritsuko
R
Ohtani-Kaneko
Hidekatsu
H
Tazawa
Makoto
M
Yokosuka
Minoru
M
Yoshida
Masahiko
M
Satoh
Chiho
C
Watanabe
D048948:14-3-3 Proteins / Q000378:metabolism; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D002147:Calmodulin / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002853:Chromatography, Liquid; D003712:Dendrites / Q000187:drug effects / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D033042:Fetal Research; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000473:pathology*; D011247:Pregnancy; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013569:Synapses / Q000187:drug effects / Q000648:ultrastructure; D053719:Tandem Mass Spectrometry
D048948:14-3-3 Proteins; D002147:Calmodulin; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; C095114:collapsin response mediator protein-2; D002104:Cadmium