Active component of Fatsia japonica enhances the transduction efficiency of Tat-SOD fusion protein both in vitro and in vivo.
It has been reported that Tat-SOD can be directly transduced into mammalian cells and skin and acts as a potential therapeutic protein in various diseases. To isolate the compound that can enhance the transduction efficiency of Tat-SOD, we screened a number of natural products. 3-O-[beta-D-Glucopyranosyl(1-->4)-alpha-L-arabinopyranosyl]- hederagenin (OGAH) was identified as an active component of Fatsia japonica and is known as triterpenoid glycosides (hederagenin saponins). OGAH enhanced the transduction efficiencies of Tat-SOD into HeLa cells and mice skin. The enzymatic activities in the presence of OGAH were markedly increased in vitro and in vivo when compared with the controls. Although the mechanism is not fully understood, we suggest that OGAH, the active component of Fatsia japonica, might change the conformation of the membrane structure and it may be useful as an ingredient in antiaging cosmetics or as a stimulator of therapeutic proteins that can be used in various disorders related to reactive oxygen species (ROS).
Lee
Sun Hwa
SH
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea.
Kim
So Young
SY
Kim
Dae Won
DW
Jang
Sang Ho
SH
Lim
Soon-Sung
SS
Kwon
Hyung Joo
HJ
Kang
Tae-Cheon
TC
Won
Moo Ho
MH
Kang
Il Jun
IJ
Lee
Kil Soo
KS
Park
Jinseu
J
Eum
Won Sik
WS
Choi
Soo Young
SY
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects* / Q000502:physiology; D015696:Gene Products, tat / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D019684:Magnoliopsida; D051379:Mice; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism*; D012503:Saponins / Q000494:pharmacology*; D012869:Skin Absorption / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism*
C098366:3-O-(glucopyranosyl-1-4-arabinopyranosyl)hederagenin; D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D012503:Saponins; D009828:Oleanolic Acid; D013482:Superoxide Dismutase