Production and accumulation of xylooligosaccharides with long chains by growing culture and xylanase of a mutant strain of Bacillus pumilus X-6-19.
Bacillus pumilus X-6-9 isolated from soil and subsequently identified, produced xylooligosaccharides with long chains from xylan and accumulated them in the culture. By improving the culture conditions and mutating the bacterium, a 3.2-fold increase in the production of the xylooligosaccharides was established, when compared to the original culture conditions of B. pumilus X-6-19. The addition of D-glucose to the culture of the mutant strain U-3 of B. pumilus X-6-9 repressed the synthesis of beta-xylosidase, but not xylanase. Thus, it was revealed that strain U-3 was a good organism for the production and accumulation of xylooligosaccharides with long chains from xylan by a microbial culture. Xylanase produced by strain U-3 was purified to homogeneity and characterized. The hydrolyzates generated by the purified xylanase contained xylobiose, xylotriose, xylotetraose, and xylopentaose, but not xylose.
Division of Life Science, Graduate School of Science and Technology, Kobe University, Rokko, Kobe 657-8501, Japan.
Qingzhu
Q
Yuan
Tsuyoshi
T
Adachi
Shinji
S
Takenaka
Shuichiro
S
Murakami
Machiko
M
Tanaka
Kenji
K
Aoki
D001407:Bacillus / Q000235:genetics* / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D009154:Mutation; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012988:Soil Microbiology
D009844:Oligosaccharides; D011994:Recombinant Proteins; D043364:Endo-1,4-beta Xylanases; D005947:Glucose