PNA based artificial nucleases displaying catalysis with turnover in the cleavage of a leukemia related RNA model.
Several peptide nucleic acid based artificial nucleases (PNAzymes) are designed to create a bulge in the target RNA, which is a short model of the leukemia related bcr/abl mRNA. The target RNA is cleaved by the PNAzymes with a half-life of down to 11 h (using a 1 : 1 ratio of PNA-conjugate to target) and only upon base-pairing with the substrate. The PNA based systems are also shown to act in a catalytic fashion with turnover of substrate and are thus the first reported peptide nucleic acid based artificial RNA-cleaving enzymes.
Murtola
Merita
M
Department of Biosciences and Nutrition, Karolinska Institutet Novum, 14157 Huddinge, Sweden.
Strömberg
Roger
R
D001483:Base Sequence; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D007938:Leukemia / Q000235:genetics*; D020135:Peptide Nucleic Acids / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*
D020135:Peptide Nucleic Acids; D012333:RNA, Messenger; D012260:Ribonucleases