[Effect of Jagged1 on the morphology of DC differentiation observed by atomic force microscopy].
OBJECTIVE
To explore the effect of Jagged1 on the morphology of dendritic cell(DC) differentiation.


METHODS
Mouse bone marrow cells were cultured in the presence of rmIL-4 and rmGM-CSF. Atomic force microscopy (AFM) was used to observe the ultrastructure of these DC.


RESULTS
Similar to the control, the cells in Jagged1 group displayed irregular shape with spiny or sheet-like dendrites and the cellular surface was rough. In contrast, the cells in DAPT group exhibited little dendritic processes and most of them were of round shape and with smooth surface. Furthermore, the height of the cells in Jagged1 group was higher than that in control and DAPT group, while the diameter of the cells in both was roughly equivalent.


CONCLUSIONS
Jagged1 has no significant effect on the morphology of DC, but significant changes in the morphology of DC in DAPT group have occurred.
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China.
Jiong-Kun
JK
Wang
Ming-Qian
MQ
Hu
Fei-Yue
FY
Xing
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D000072100:Jagged-1 Protein; D008297:Male; D008565:Membrane Proteins / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018625:Microscopy, Atomic Force / Q000379:methods*; D000072099:Serrate-Jagged Proteins; D014280:Triglycerides / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606274:Jag1 protein, mouse; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D000072099:Serrate-Jagged Proteins; D014280:Triglycerides; D005680:gamma-Aminobutyric Acid; C057077:1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol