Cloning and functional analysis of wheat V-H+-ATPase subunit genes.
The root microsomal proteomes of salt-tolerant and salt-sensitive wheat lines under salt stress were analyzed by two-dimensional electrophoresis and mass spectrum. A wheat V-H(+)-ATPase E subunit protein was obtained whose expression was enhanced by salt stress. In silicon cloning identified the full-length cDNA sequences of nine subunits and partial cDNA sequences of two subunits of wheat V-H(+)-ATPase. The expression profiles of these V-H(+)-ATPase subunits in roots and leaves of both salt-tolerant and salt-sensitive wheat lines under salt and abscisic acid (ABA) stress were analyzed. The results indicate that the coordinated enhancement of the expression of V-H(+)-ATPase subunits under salt and ABA stress is an important factor determining improved salt tolerance in wheat. The expression of these subunits was tissue-specific. Overexpression of the E subunit by transgenic Arabidopsis thaliana was able to enhance seed germination, root growth and adult seedling growth under salt stress.
Zhao
Qian
Q
College of Life Science, Hebei Normal University, Shijiazhuang 050016, People's Republic of China.
Zhao
Yan-Jun
YJ
Zhao
Bao-Cun
BC
Ge
Rong-Chao
RC
Li
Ming
M
Shen
Yin-Zhu
YZ
Huang
Zhan-Jing
ZJ
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D017343:Genes, Plant; D006180:Proton-Translocating ATPases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
D017931:DNA Primers; D006180:Proton-Translocating ATPases