Increased degradation rate of nitrososureas in media containing carbonate.
The stability of two nitrosoureas, tauromustine and lomustine, has been investigated in different media and buffers. All media tested, except Leibovitz's L-15 medium, significantly increased the degradation rate of the investigated nitrosoureas at pH 7.4. Sodium bicarbonate seems to be the cause of the observed increase of the degradation rate, since it provides the main buffering capacity of all the media except for Leibovitz's L-15 medium, which is based on phosphate buffer. Other ingredients in the media, such as amino acids, vitamins, and inorganic salts, or the ionic strength of a buffer, did not have any major effect on the degradation rate of the nitrosoureas. These results suggest that media containing carbonated buffer should be avoided when the anti-tumor effect of nitrosoureas is to be investigated in different cell cultures.
Department of Preclinical Development, Active Biotech Research AB, P.O. Box 724, 220 07 Lund, Sweden.
Janeric
J
Seidegård
Lena
L
Grönquist
Helen
H
Tuvesson
Per-Olov
PO
Gunnarsson
D002021:Buffers; D002254:Carbonates / Q000378:metabolism*; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D006531:HEPES; D008130:Lomustine / Q000378:metabolism; D009607:Nitrosourea Compounds / Q000378:metabolism*; D013654:Taurine / Q000031:analogs & derivatives / Q000378:metabolism
D002021:Buffers; D002254:Carbonates; D003470:Culture Media; D009607:Nitrosourea Compounds; D013654:Taurine; C052991:1-(2-chloroethyl)-3-(2-(dimethylaminosulfonyl)ethyl)-1-nitrosourea; D008130:Lomustine; D006531:HEPES