The genetic network controlling the Arabidopsis transcriptional response to Pseudomonas syringae pv. maculicola: roles of major regulators and the phytotoxin coronatine.
Expression profiling of wild-type plants and mutants with defects in key components of the defense signaling network was used to model the Arabidopsis network 24 h after infection by Pseudomonas syringae pv. maculicola ES4326. Results using the Affymetrix ATH1 array revealed that expression levels of most pathogen-responsive genes were affected by mutations in coi1, ein2, npr1, pad4, or sid2. These five mutations defined a small number of different expression patterns displayed by the majority of pathogen-responsive genes. P. syringae pv. tomato strain DC3000 elicited a much weaker salicylic acid (SA) response than ES4326. Additional mutants were profiled using a custom array. Profiles of pbs3 and ndr1 revealed major effects of these mutations and allowed PBS3 and NDR1 to be placed between the EDS1/PAD4 node and the SA synthesis node in the defense network. Comparison of coi1, dde2, and jar1 profiles showed that many genes were affected by coi1 but very few were affected by dde2 or jar1. Profiles of coi1 plants infected with ES4326 were very similar to those of wild-type plants infected with bacteria unable to produce the phytotoxin coronatine, indicating that, essentially, all COI1-dependent gene expression changes in this system are caused by coronatine.
Wang
Lin
L
Department of Plant Biology, Microbial and Plant Genomics Institute, University of Minnesota, 1445 Gortner Avenue, St. Paul 55108, USA.
Mitra
Raka M
RM
Hasselmann
Keegan D
KD
Sato
Masanao
M
Lenarz-Wyatt
Lisa
L
Cohen
Jerry D
JD
Katagiri
Fumiaki
F
Glazebrook
Jane
J
D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks / Q000502:physiology; D018745:Genome, Plant; D054884:Host-Pathogen Interactions; D019751:Intramolecular Transferases / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; D002265:Carboxylic Ester Hydrolases; D019751:Intramolecular Transferases; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C112279:COI1 protein, Arabidopsis; C119963:EIN2 protein, Arabidopsis; C109369:NDR1 protein, Arabidopsis; C523087:At5g13320 protein, Arabidopsis; C400766:PAD4 protein, Arabidopsis; C058486:isochorismate synthase