Protein Standard Absolute Quantification (PSAQ) for improved investigation of staphylococcal food poisoning outbreaks.
Staphylococcal enterotoxins are major causing agents of food-borne diseases. Their detection in food remnants for risk assessment or food poisoning outbreaks investigation suffers from a lack in comprehensive immunological tools. In this study, we demonstrate that the combination of immunocapture and Protein Standard Absolute Quantification (PSAQ) strategy, which uses isotope-labeled enterotoxins as internal standards for MS-based analysis, is powerful to specifically identify and quantify these contaminating agents in food matrices. This approach is believed to significantly improve the elucidation of staphylococcal food poisoning outbreaks.
CEA, DSV, iRTSV, Laboratoire d'Etude de la Dynamique des Protéomes, Grenoble, France.
Alain
A
Dupuis
Jacques-Antoine
JA
Hennekinne
Jérôme
J
Garin
Virginie
V
Brun
D002853:Chromatography, Liquid / Q000379:methods; D004196:Disease Outbreaks; D004768:Enterotoxins / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000378:metabolism; D012015:Reference Standards; D013202:Staphylococcal Food Poisoning / Q000453:epidemiology*; D013211:Staphylococcus aureus / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism
D004768:Enterotoxins; D010455:Peptides; C026066:enterotoxin A, Staphylococcal; D014357:Trypsin