Histopathological lesions following intramuscular administration of saline in laboratory rodents and rabbits.
This review was undertaken to assess the nature and incidence of procedure-related changes in mice, rats and rabbits which received saline solution by intramuscular injection. Data were collected on the injection sites from 7 studies representing 152 animals. The original observations by the different study pathologists from both control and treated animals were evaluated in order to create a glossary of preferred terms to be used in toxicology studies. These standardized terms were then applied to changes observed in the saline-treated animals. The review showed that the most severe of the procedure-related lesions were only of a slight level. Two days post-injection, the local reactions were mainly composed of minimal infiltration by mononuclear cells (lymphocytes and macrophages) with occasional degeneration of myofibres. From 10 to 42 days post-injection, lesions showed regeneration of myofibres and some fibrosis. In rats, the number of injections at each site influenced inflammatory infiltrate and degenerative lesions.
Thuilliez
Celine
C
MDS Pharma Services, Drug Safety Assessment, Les Oncins, Saint-Germain-Sur-L'Arbresle, France.
Dorso
Laetitia
L
Howroyd
Paul
P
Gould
Sarah
S
Chanut
Franck
F
Burnett
Roger
R
D000818:Animals; D005260:Female; D007249:Inflammation / Q000139:chemically induced* / Q000473:pathology; D007273:Injections, Intramuscular; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000473:pathology*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000008:administration & dosage / Q000633:toxicity*
D012965:Sodium Chloride