Effects of nicergoline on corneal epithelial wound healing in rat eyes.
OBJECTIVE
To investigate the effect of nicergoline on corneal epithelial wound healing in rats.


METHODS
One hundred Sprague-Dawley male rats were divided into two groups, the control group and the nicergoline-treated group, for 2 weeks. Corneal wound healing was evaluated by fluorescein staining after epithelial debridement. Nerve growth factor (NGF) protein and NGF mRNA were measured in rat corneas by ELISA and RT-PCR. NGF concentration of lacrimal gland was also evaluated by means of ELISA. Immunofluorescent staining was performed in rat corneas.


RESULTS
The corneal wound healing rate was increased in nicergoline-treated rats compared with control rats after debridement. Twenty-four hours after epithelial debridement, corneal NGF protein and NGF mRNA levels were higher in the nicergoline-treated group than in the control group. Immunofluorescent staining showed that NGF staining was stronger in nicergoline-treated corneas than in control corneas 24 hours after epithelial debridement. In addition, NGF concentrations in lacrimal glands of the nicergoline-treated group were significantly higher than in the control group 24 hours after epithelial debridement.


CONCLUSIONS
Nicergoline accelerated wound healing in rat corneas. The promoting effect of nicergoline in corneal wound healing is likely to be related to increased NGF in corneas and lacrimal glands.
Department of Ophthalmology and Visual Science, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Su-Young
SY
Kim
Jun-Sub
JS
Choi
Choun-Ki
CK
Joo
D000818:Animals; D003646:Debridement; D004797:Enzyme-Linked Immunosorbent Assay; D019573:Epithelium, Corneal / Q000166:cytology / Q000293:injuries / Q000502:physiology*; D019084:Fluorescent Antibody Technique, Indirect; D007765:Lacrimal Apparatus / Q000378:metabolism; D008297:Male; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D009530:Nicergoline / Q000494:pharmacology*; D018697:Nootropic Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014945:Wound Healing / Q000187:drug effects*
D018697:Nootropic Agents; D012333:RNA, Messenger; D020932:Nerve Growth Factor; D009530:Nicergoline