[Effect of HBV X gene on mitochondria in HL-7702 cells].
OBJECTIVE
To study the effect of HBx on a new HBx-interacted protein COXIII and mitochondria.


METHODS
A recombinant eukaryotic expression plasmid pcDNA3-X was constructed, then pcDNA3-X and pcDNA3 were steadily transfected in HL-7702 separately, named HL-7702/HBx and HL-7702/pcDNA(3). The expression of COXIII in HL-7702/HBx, HL-7702/pcDNA3 and HL-7702 cells were detected by RT-PCR and western blot. Mitochondrial cytochrome c oxidase activities of three groups were assayed spectrophotometrically at 550 nm by using partially purified mitochondria.


RESULTS
COXIII mRNA expression didn't change, while protein expression is down-regulated in HL-7702/HBx. Cytochrome c oxidase activity was much lower in HL-7702/HBx compared with HL-7702 and HL-7702/pcDNA3 cells.


CONCLUSIONS
HBx can down-regulate COXIII expression through post-transcriptional level and inhibit mitochondrial cytochrome c oxidase activity.
Lin
Na
N
Department of Gastroenterology, Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Li
Dan
D
Chen
Hong-Ying
HY
Chen
Zhi-Xin
ZX
Wang
Xiao-Zong
XZ
D002460:Cell Line; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins
D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D003576:Electron Transport Complex IV