Cathepsin K regulates adipocyte differentiation: possible involvement of type I collagen degradation.
We previously found that cathepsin K (CTSK) played an important role in adipocyte differentiation. However, the underlying molecular mechanism is not clear. Through the time window study, it was observed that CTSK activities were required mainly in the early phases of adipogenic process. At the same time, the expression of type I collagen disappeared. However, type I collagen can still be observed during the whole process when the CTSK inhibitor-E64 was added. The mRNA levels of peroxisome proliferator-activated receptor gamma (PPAR-gamma) and CCAAT/enhancer binding protein alpha (C/EBP-alpha) was also declining. These imply that CTSK may play a role in adipogenesis in early differentiation phases and produce an effect at least partly by degrading type I collagen, which may provides a basis for developing novel therapeutic approaches to treat obesity and the diseases associated with it.
Endocrinology & Metabolism, Shanghai Jiaotong University affiliated No.6 People Hospital, Shanghai Diabetes Institute, China.
Junfeng
J
Han
Tianhong
T
Luo
Yanyun
Y
Gu
Guo
G
Li
Weiping
W
Jia
Min
M
Luo
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D056657:Cathepsin K; D002403:Cathepsins / Q000037:antagonists & inhibitors / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D024042:Collagen Type I / Q000378:metabolism* / Q000502:physiology; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D024042:Collagen Type I; D015853:Cysteine Proteinase Inhibitors; D047495:PPAR gamma; D002403:Cathepsins; D056657:Cathepsin K; C534132:Ctsk protein, mouse; D007930:Leucine; C024974:E 64