Following the biocatalytic activities of glucose oxidase by electrochemically cross-linked enzyme-Pt nanoparticles composite electrodes.
An integrated platinum nanoparticles (NPs)/glucose oxidase (GOx) composite film associated with a Au electrode is used to follow the biocatalytic activities of the enzyme. The film is assembled on a Au electrode by the electropolymerization of thioaniline-functionalized Pt NPs and thioaniline-modified GOx. The resulting enzyme/Pt NPs-functionalized electrode stimulates the O 2 oxidation of glucose to gluconic acid and H 2O 2. The modified electrode is then implemented to follow the activity of the enzyme by the electrochemical monitoring of the generated H 2O 2. The effect of the composition of the Pt NPs/GOx cross-linked nanostructures and the optimal conditions for the preparation of the electrodes are discussed.
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Lily
L
Bahshi
Marco
M
Frasconi
Ran
R
Tel-Vered
Omer
O
Yehezkeli
Itamar
I
Willner
D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D005949:Glucose Oxidase / Q000032:analysis* / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D010984:Platinum / Q000737:chemistry*
D003432:Cross-Linking Reagents; D010984:Platinum; D005949:Glucose Oxidase