Absence of association between COL1A1 polymorphisms and high myopia in the Japanese population.
OBJECTIVE
The collagen type I alpha 1 (COL1A1) gene was recently reported to be associated with high myopia in the Japanese population. To validate this positive association, the tag single-nucleotide polymorphism (tSNP) approach was used.


METHODS
Eight tSNPs, including rs2075555 and rs2269336 (previously reported to be high myopia-susceptible SNPs in the Japanese), were selected to tag the linkage disequilibrium blocks harboring the COL1A1. These tSNPs were genotyped by using an SNP assay. A total of 427 unrelated Japanese cases with high myopia (axial length, >or=26.50 mm in both eyes; the refraction of the 644 phakic eyes ranged from -5.0 to -36.0 D, with a mean +/- SD of -13.61+/-4.20 D) and 420 Japanese control subjects were recruited. Genotype and allele distributions were compared between the cases and controls by using the chi(2) test, with multiple testing corrections performed by the permutation test.


RESULTS
There was no association noted between high myopia and rs2075555 (P=0.47, P(c)>0.99) and rs2269336 (P=0.40, P(c)>0.99). Meta-analysis of a previous Japanese study and new data obtained in a fixed-effect model indicated a mild significant association of high myopia with rs2075555 (odds ratio [OR], 1.19; 95% confidence interval [CI], 1.03-1.38, P=0.022) and rs2269336 (OR, 1.18; 95% CI, 1.02-1.36, P=0.026). No significant associations were seen with further tSNPs tests.


CONCLUSIONS
This study did not replicate the previously reported positive association between COL1A1 and high myopia in the Japanese population, and thus the genetic risk associated with this gene, if any, is weaker than originally reported.
Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Sakyo, Kyoto, Japan.
Hideo
H
Nakanishi
Ryo
R
Yamada
Norimoto
N
Gotoh
Hisako
H
Hayashi
Atsushi
A
Otani
Akitaka
A
Tsujikawa
Kenji
K
Yamashiro
Noriaki
N
Shimada
Kyoko
K
Ohno-Matsui
Manabu
M
Mochizuki
Masaaki
M
Saito
Kuniharu
K
Saito
Tomohiro
T
Iida
Fumihiko
F
Matsuda
Nagahisa
N
Yoshimura
D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D024042:Collagen Type I / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007564:Japan / Q000453:epidemiology; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D047728:Myopia, Degenerative / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
C000716127:COL1A1 protein, human; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain