The pharmacology study of a new recombinant human VEGF receptor-fc fusion protein on experimental choroidal neovascularization.
OBJECTIVE
KH902, a recombinant fusion protein, is designed for treatment of neovascular age-related macular degeneration. The study is to investigate the prevention efficacy of KH902 on experimental choroidal neovascularization (CNV) in a monkey model.


METHODS
Binding assay and endothelial cell proliferation assay were used to evaluate activity and bioactivity of KH902 in vitro while an initial comparison of bioactivity was made between KH902 and Ranizumab (Lucentis). Ocular and systemic levels of KH902 were analyzed by enzyme-linked immunosorbent assay (ELISA) method after single intravitreal administration to evaluate its availability to ocular fundus. In vivo pharmacological study, CNV was induced by laser in monkeys and KH902 prevention efficacy on CNV was evaluated by incidence of CNV and several ophthalmic examinations.


RESULTS
KH902 is a unique fusion protein with high affinity to VEGF and good availability to target tissue, beneficial to good bioactivity in vivo. In vivo pharmacological study, the incidence of CNV formation was largely reduced in KH902 treatment groups. Furthermore, the leakage of CNV in control group which crossed over to KH902 treatment 40 days after laser was much less than that before KH902 treatment.


CONCLUSIONS
KH902 was effective to prevent the formation of experimental CNV and also to treat pre-existed CNV without evidence of toxicity. This study suggests that KH902 has promise as a local anti-angiogenic treatment of CNV-related diseases.
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Ming
M
Zhang
Dechao
D
Yu
Chun
C
Yang
Qingjie
Q
Xia
Wang
W
Li
Bin
B
Liu
Hong
H
Li
D020533:Angiogenesis Inhibitors / Q000493:pharmacokinetics / Q000494:pharmacology*; D000792:Angiography; D000818:Animals; D019540:Area Under Curve; D049109:Cell Proliferation / Q000187:drug effects; D002829:Choroid / Q000473:pathology; D020256:Choroidal Neovascularization / Q000473:pathology / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005123:Eye / Q000473:pathology; D005451:Fluorescein Angiography; D007839:Functional Laterality / Q000502:physiology; D007141:Immunoglobulin Fc Fragments / Q000494:pharmacology*; D007267:Injections; D008024:Ligands; D008253:Macaca mulatta; D040262:Receptors, Vascular Endothelial Growth Factor / Q000187:drug effects* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000009:adverse effects / Q000493:pharmacokinetics / Q000494:pharmacology*
D020533:Angiogenesis Inhibitors; D007141:Immunoglobulin Fc Fragments; D008024:Ligands; D011993:Recombinant Fusion Proteins; C527363:KH902 fusion protein; D040262:Receptors, Vascular Endothelial Growth Factor