Polyclonal antibody to porcine p53 protein: a new tool for studying the p53 pathway in a porcine model.
Although the tumor suppressor protein p53 is important in the control of various cellular activities, the analysis of p53 in the porcine model has been hampered by a lack of a suitable antibody that is specific for porcine p53. Using a recombinant porcine p53, we generated a rabbit polyclonal antibody (designated SH0797) that is directed against porcine p53. The results of the study show that the antibody is capable of detecting recombinant p53 protein expressed in Escherichia coli, as well as FLAG-tagged p53 that is expressed in the transfected cells. This demonstrates that the antibody is specific for the porcine p53 protein. The antibody also showed the ability to immunoprecipitate p53 protein from extracts of porcine cells and to cross-react with human p53 protein. In addition, expression of porcine p53 could be induced readily in porcine cells and detected using this new tool. This antibody is a useful tool for use in studies of the cellular pathways that involve p53 in the porcine model.
Qiu
Yafeng
Y
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No. 518, Ziyue Road, Shanghai, 200241, PR China.
Shen
Yang
Y
Li
Xiangdong
X
Liu
Qingwei
Q
Ma
Zhiyong
Z
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000906:Antibodies / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D004317:Doxorubicin / Q000494:pharmacology; D047468:Immunoprecipitation; D023421:Models, Animal; D015398:Signal Transduction; D013552:Swine / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D000906:Antibodies; D016159:Tumor Suppressor Protein p53; D004317:Doxorubicin