Deoxyribonuclease activity and circulating DNA concentration in blood plasma of patients with prostate tumors.
The DNase activity and circulating DNA (cirDNA) concentration in blood plasma of healthy donors, patients with chronic prostatitis, and patients with prostate tumors were analyzed. The concentration of the cirDNA from plasma was determined by PicoGreen fluorescent assay. DNase activity in blood was measured using the immunoassay based on the cleavage of a hapten-labeled 974-bp DNA substrate. The mean cirDNA concentration in the plasma of healthy donors was low (21 +/- 4 ng/mL total blood) and was accompanied by high DNase activity (0.17 +/- 0.04 U/mL blood). The mean cirDNA concentration was 90 ng/mL blood (10-234 ng/mL) in the patients with nonmalignant prostate tumors and 115 ng/mL blood (13-339 ng/mL) in those with prostate cancer. The mean DNase activity in blood plasma of the patients with prostate tumors was 0.06 U/mL blood (0-0.12 U/mL). The results obtained demonstrate that increased concentrations of cirDNA in blood of the patients with prostate tumors is accompanied by a decreased DNase activity, confirming our previous data that a low DNase activity in blood plasma of cancer patients is one reason for a high cirDNA concentration.
Cherepanova
Anna V
AV
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Tamkovich
Svetlana N
SN
Bryzgunova
Olga E
OE
Vlassov
Valentin V
VV
Laktionov
Pavel P
PP
D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D004247:DNA / Q000097:blood*; D004273:DNA, Neoplasm / Q000097:blood; D003851:Deoxyribonucleases / Q000097:blood*; D006801:Humans; D008297:Male; D011470:Prostatic Hyperplasia / Q000097:blood / Q000201:enzymology / Q000235:genetics; D011471:Prostatic Neoplasms / Q000097:blood / Q000201:enzymology / Q000235:genetics
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D004247:DNA; D003851:Deoxyribonucleases