The impact of staphylococcal mastitis on the level of milk IL-6, lysozyme and nitric oxide.
Mammary gland secretions derived from secretory cows infected with coagulase +ve Staphylococcus spp. was examined for the expression of IL-6, production of lysozyme and NO(x). The examined cows reflected 25 cases of subclinical mastitis and 15 cases of clinically mastitic animals. The IL-6 concentration in the subclinical animals was significantly higher (30.8 ng/ml) than the clinically manifested animals (18.0 ng/ml) and the normal cows (5.2n g/ml). On the other hand the level of lysozyme although significantly higher than the normal cows (6.9 microg/ml) yet its level in the subclinical animals (11.2 microg/ml) was lower than that estimated in the clinical animals (15.6 microg/ml). Similarly, the level of NO(x) in the normal animals was found to be 5.6 microM/ml to increase to 6.2 microM/ml in the subclinical mastitic animals and to significantly increase further to 11.5 microM/ml in the clinically affected cows. These results suggest the promising use of whey IL-6, lysozyme or/and NO concentration variabilities as prognostic parameters on the degree of the commencement of mastitis in cows.
Osman
Kamelia M
KM
Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt. s_mougy@hotmail.com
Hassan
Hany M
HM
Ibrahim
Ihab M
IM
Mikhail
Magda M S
MM
D000818:Animals; D002417:Cattle; D005260:Female; D015850:Interleukin-6 / Q000032:analysis*; D007774:Lactation / Q000276:immunology; D008321:Mammary Glands, Animal / Q000276:immunology / Q000382:microbiology; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D008892:Milk / Q000276:immunology* / Q000382:microbiology; D009113:Muramidase / Q000032:analysis*; D009569:Nitric Oxide / Q000032:analysis*; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D013210:Staphylococcus / Q000276:immunology
D015850:Interleukin-6; D009569:Nitric Oxide; D009113:Muramidase