RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model.
A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C 8 column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity ( R (2), 0.994-1.000), limit of detection (0.08-1.28 microg/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory. The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression.
Hu
Chunxiu
C
Division of Analytical Biosciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
van Dommelen
Judith
J
van der Heijden
Rob
R
Spijksma
Gerwin
G
Reijmers
Theo H
TH
Wang
Mei
M
Slee
Elizabeth
E
Lu
Xin
X
Xu
Guowang
G
van der Greef
Jan
J
Hankemeier
Thomas
T
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D017357:Cyclotrons; D008055:Lipids / Q000097:blood*; D051379:Mice; D008817:Mice, Mutant Strains; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D016159:Tumor Suppressor Protein p53 / Q000097:blood*
D008055:Lipids; D016159:Tumor Suppressor Protein p53