SSCP analysis of scnDNA for genetic profiling of Aedes aegypti.
We characterized genetic profiling markers for Aedes aegypti using single-strand conformation polymorphism (SSCP) analysis of single copy nuclear genes (scnDNA). Nucleotide variations at 18 loci were evaluated in 173 wild Ae. aegypti collected from a single population in northwestern Thailand. We identified seven scnDNAs with polymorphisms sufficient to determine a unique genetic profile for each mosquito examined. Six markers were derived from previously mapped cDNA loci. One marker was developed from a non-coding region of a gene. The number of alleles at each scnDNA locus ranged from 3 to 9. The described scnDNAs can be used to quickly fingerprint large numbers of Ae. aegypti to track the behavior of individual mosquitoes in the field.
Department of Entomology, University of California, Davis, California 95616, USA. jacwong@ucdavis.edu
Jacklyn
J
Wong
Frédéric
F
Tripet
Jason L
JL
Rasgon
Gregory C
GC
Lanzaro
Thomas W
TW
Scott
D000330:Aedes / Q000235:genetics* / Q000502:physiology; D000483:Alleles; D000818:Animals; D004247:DNA / Q000235:genetics*; D018628:Gene Dosage; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010058:Oviposition; D018807:Polymorphism, Single-Stranded Conformational
D005819:Genetic Markers; D004247:DNA