Inhibition of G1P3 expression found in the differential display study on respiratory syncytial virus infection.
BACKGROUND
Respiratory syncytial virus (RSV) is the leading viral pathogen associated with bronchiolitis and lower respiratory tract disease in infants and young children worldwide. The respiratory epithelium is the primary initiator of pulmonary inflammation in RSV infections, which cause significant perturbations of global gene expression controlling multiple cellular processes. In this study, differential display reverse transcription polymerase chain reaction amplification was performed to examine mRNA expression in a human alveolar cell line (SPC-A1) infected with RSV.


RESULTS
Of the 2,500 interpretable bands on denaturing polyacrylamide gels, 40 (1.6%) cDNA bands were differentially regulated by RSV, in which 28 (70%) appeared to be upregulated and another 12 (30%) appeared to be downregulated. Forty of the expressed sequence tags (EST) were isolated, and 20 matched homologs in GenBank. RSV infection upregulated the mRNA expression of chemokines CC and CXC and interfered with type alpha/beta interferon-inducible gene expression by upregulation of MG11 and downregulation of G1P3.


CONCLUSIONS
RSV replication could induce widespread changes in gene expression including both positive and negative regulation and play a different role in the down-regulation of IFN-alpha and up-regulation of IFN-gamma inducible gene expression, which suggests that RSV interferes with the innate antiviral response of epithelial cells by multiple mechanisms.
Pediatrics Department, Zhongnan Hospital of Wuhan University Medical School, Donghu Road 169, Wuhan 430071, PR China. zhaodong@public.wh.hb.cn
Dongchi
D
Zhao
Dan
D
Peng
Lei
L
Li
Qiwei
Q
Zhang
Chuyu
C
Zhang
D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000821:virology*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D020559:Monomeric GTP-Binding Proteins / Q000096:biosynthesis; D012136:Respiratory Syncytial Viruses / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000076106:SAM Domain and HD Domain-Containing Protein 1
C468762:IFI6 protein, human; D024101:Mitochondrial Proteins; D000076106:SAM Domain and HD Domain-Containing Protein 1; C484280:SAMHD1 protein, human; D020559:Monomeric GTP-Binding Proteins