Immunosuppressive pregnane glycosides from Periploca sepium and Periploca forrestii.
Nine pregnane glycosides containing peroxy functions in their sugar moieties (1-5 and 11-14), five oligosaccharides (6-10), six pregnane glycosides (15-20), and five cardiac glycosides (21-25) were isolated from the root barks of Periploca sepium Bge. (Asclepiadaceae) and the roots of Periploca forrestii Schltr. (Asclepiadaceae), two traditional Chinese medicines used for the treatment of rheumatoid arthritis. Among them, 1-8 are hitherto unknown. Their structures were characterized on the basis of spectroscopic analyses. In pharmacological testing, compounds 1-5 and 11-14 were found to exhibit inhibitory activity against the proliferation of T lymphocyte in vitro with IC50 values ranging from 0.29microM to 1.97microM, while the other components showed no significant inhibitory activity.
Feng
Jiaquan
J
Department of Natural Products Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
Zhang
Rujun
R
Zhou
Yu
Y
Chen
Zhenhua
Z
Tang
Wei
W
Liu
Qunfang
Q
Zuo
Jian-Ping
JP
Zhao
Weimin
W
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D031109:Periploca / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D011278:Pregnanes / Q000737:chemistry*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
D006027:Glycosides; D007166:Immunosuppressive Agents; D011278:Pregnanes