[Technological process of cell disruption for extracting astaxanthin from Phaffia rhodozyma by acid method under autoclave conditions].
Phaffia rhodozyma is one of the organisms for production of astaxanthin, and the key process for extracting intracellular astaxanthin is cell disruption. In this work, cell disruption for extracting astaxanthin from Phaffia rhodozyma was studied with autoclave method at low acid concentration. The optimum disrupting conditions were: autoclave pressure 0.1 MPa, 121 degrees C; hydrochloric acid concentration 0.5 mol/L; liquid to material ratio (V/W) 30 mL/g dry cell weight and disruption time 2 min. Under the optimum conditions, medium scale experiment showed that astaxanthin and total carotenoids recovery from Phaffia rhodozyma were (84.8 +/- 3.2)% and (93.3 +/- 2)%, respectively. This new method can lead to no poisonous residues and get high extraction yield, which have good prospects to be put into industrial production.
Lu
Baoju
B
College of Bioengineering, Jimei University, Xiamen 361021, China.
Xiao
Anfeng
A
Lil
Lijun
L
Ni
Hui
H
Cai
Huinong
H
Su
Wenjin
W
D001487:Basidiomycota / Q000737:chemistry*; D002338:Carotenoids / Q000302:isolation & purification; D002473:Cell Wall / Q000378:metabolism; D006358:Hot Temperature; D006851:Hydrochloric Acid; D024341:Xanthophylls / Q000302:isolation & purification
D024341:Xanthophylls; D002338:Carotenoids; C005948:astaxanthine; D006851:Hydrochloric Acid