New insight into the surface denaturation of proteins: electronic sum frequency generation study of cytochrome c at water interfaces.
In situ characterization of surface denaturation of a protein was realized by newly developed interface-selective multiplex electronic sum frequency generation spectroscopy. The observed electronic spectra of cytochrome c at the air/water interface exhibited a broad feature, which demonstrated coexistence of the nativelike and denatured protein at the interface. This situation of the mixed conformation at the air/water interface did not change in the acidic condition of pH=2 where the protein was completely denatured in the bulk water. In sharp contrast, only native spectrum was observed at the silica/water interface.
Pratik
P
Sen
Shoichi
S
Yamaguchi
Tahei
T
Tahara
D000818:Animals; D045304:Cytochromes c / Q000737:chemistry*; D004583:Electrons; D006736:Horses; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D011489:Protein Denaturation; D013057:Spectrum Analysis; D014867:Water / Q000737:chemistry
D014867:Water; D045304:Cytochromes c