Oropouche virus entry into HeLa cells involves clathrin and requires endosomal acidification.
Oropouche virus (ORO), family Bunyaviridae, is the second most frequent cause of arboviral febrile illness in Brazil. Studies were conducted to understand ORO entry in HeLa cells. Chlorpromazine inhibited early steps of ORO replication cycle, consistent with entry/uncoating. The data indicate that ORO enters HeLa cells by clathrin-coated vesicles, by a mechanism susceptible to endosomal acidification inhibitors. Transmission electron microscopy and immunofluorescence indicated that ORO associates with clathrin-coated pits and can be found in association with late endosomes in a time shorter than 1h.
Department of Cell and Molecular Biology, University of São Paulo School of Medicine at Ribeirão Preto, Brazil. risantos@usp.br
Rodrigo I M
RI
Santos
Alcir H
AH
Rodrigues
Maria Lúcia
ML
Silva
Renato A
RA
Mortara
Marcos A
MA
Rossi
Maria Célia
MC
Jamur
Constance
C
Oliver
Eurico
E
Arruda
D000143:Acids / Q000378:metabolism*; D001938:Brazil; D002044:Bunyaviridae Infections / Q000378:metabolism* / Q000821:virology; D022163:Clathrin-Coated Vesicles / Q000378:metabolism* / Q000648:ultrastructure / Q000821:virology; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure / Q000821:virology; D006367:HeLa Cells; D006801:Humans; D016851:Orthobunyavirus / Q000502:physiology* / Q000648:ultrastructure; D053586:Virus Internalization
D000143:Acids