Reconfiguration of genomic anchors upon transcriptional activation of the human major histocompatibility complex.
The folding of chromatin into topologically constrained loop domains is essential for genomic function. We have identified genomic anchors that define the organization of chromatin loop domains across the human major histocompatibility complex (MHC). This locus contains critical genes for immunity and is associated with more diseases than any other region of the genome. Classical MHC genes are expressed in a cell type-specific pattern and can be induced by cytokines such as interferon-gamma (IFNG). Transcriptional activation of the MHC was associated with a reconfiguration of chromatin architecture resulting from the formation of additional genomic anchors. These findings suggest that the dynamic arrangement of genomic anchors and loops plays a role in transcriptional regulation.
Ottaviani
Diego
D
Cancer Research UK London Research Institute, Lincoln's Inn Fields, London WC2A 3PX, United Kingdom.
Lever
Elliott
E
Mitter
Richard
R
Jones
Tania
T
Forshew
Tim
T
Christova
Rossitza
R
Tomazou
Eleni M
EM
Rakyan
Vardhman K
VK
Krawetz
Stephen A
SA
Platts
Adrian E
AE
Segarane
Badmavady
B
Beck
Stephan
S
Sheer
Denise
D
D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology; D005802:Genes, MHC Class II; D015894:Genome, Human; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008285:Major Histocompatibility Complex; D045170:Matrix Attachment Regions / Q000187:drug effects; D008957:Models, Genetic; D011994:Recombinant Proteins; D015533:Transcriptional Activation
D002843:Chromatin; D006680:HLA Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma