Molecular analysis of recombinase-mediated cassette exchange reactions catalyzed by integrase of coliphage HK022.
The integrase (Int) protein of coliphage HK022 can catalyze in Escherichia coli as well as in in vitro integrative and excisive recombinase-mediated cassette exchange reactions between plasmids as substrates. Atomic force microscopy images have revealed that in the protein-DNA complexes that are formed, the plasmid substrates are connected via one and not two pairs of attachment sites. This observation, together with the elucidation of intermediate co-integrates between the two circular plasmids, suggest that a sequential mechanism of the RMCE reaction is possible.
Department of Biochemistry, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.
Natalia
N
Malchin
Tatiana
T
Molotsky
Ezra
E
Yagil
Alexander B
AB
Kotlyar
Mikhail
M
Kolot
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001287:Attachment Sites, Microbiological; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D025523:Bacteriophage HK022 / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D055162:Biocatalysis; D002701:Chloramphenicol / Q000494:pharmacology; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics; D048168:Escherichia coli K12 / Q000187:drug effects / Q000235:genetics / Q000821:virology*; D005821:Genetic Techniques; D019426:Integrases / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D016662:Virus Integration
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002701:Chloramphenicol; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C077570:Site-specific recombinase