Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities.
This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH(4))(2)SO(4) precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity.
Shahid
Muhammad
M
Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan.
Tayyab
Muhammad
M
Naz
Farah
F
Jamil
Amer
A
Ashraf
Muhammad
M
Gilani
Anwarul Hassan
AH
D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D029802:Croton / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D010936:Plant Extracts / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010936:Plant Extracts