Cytokine response to Escherichia coli in gnotobiotic pigs.
One-week-old-germ-free pigs were inoculated with 10(8) CFU of E.coli bacteria-either commensal 086 strain or virulent 055 strain for 1 d. Bacteria were counted in the small intestine, mesenteric lymph nodes, blood and lungs. The O55 strain reached higher levels in circulation and lungs. IL-8, IL-10 and TNF-alpha concentrations were determined by ELISA in plasma and intestinal washes . No difference in cytokine levels was found between control germ-free pigs and their counterparts associated with commensal O86 strain in spite of its high concentration in the gut and circulation.
Splichal
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Division of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Czechia. splichal@gnotobio.cz
Splichalova
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Trebichavsky
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D000818:Animals; D016207:Cytokines / Q000032:analysis* / Q000097:blood; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000276:immunology* / Q000382:microbiology; D005856:Germ-Free Life; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D013552:Swine
D016207:Cytokines