Lanthanide-based protease activity sensors for time-resolved fluorescence measurements.
Lanthanide-based luminescent sensors are noteworthy because their long-lifetime luminescence enables time-resolved fluorescence measurements. After exploring suitable antenna groups, we designed and synthesized lanthanide-based luminogenic sensors detecting protease activities. This sensor yielded strong luminescence on addition of proteases such as calpain I and leucine aminopeptidase (LAP). Since the luminescence lifetimes of the probes were very long, the sensors could be applied to time-resolved measurements that exclude background fluorescence signals derived from proteins or other impurities. This sensing principle could be applicable to general time-resolved fluorescence assays for other proteases.
Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Shin
S
Mizukami
Kazuhiro
K
Tonai
Masahiro
M
Kaneko
Kazuya
K
Kikuchi
D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002154:Calpain / Q000032:analysis / Q000378:metabolism; D002614:Chelating Agents / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D007931:Leucyl Aminopeptidase / Q000032:analysis / Q000378:metabolism; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry; D010447:Peptide Hydrolases / Q000032:analysis* / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013725:Terbium / Q000737:chemistry*
D000814:Aniline Compounds; D002614:Chelating Agents; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D013725:Terbium; D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase; D002154:Calpain