Evaluation of carrier ampholyte-based capillary electrophoresis for separation of peptides and peptide mimetics.
Carrier ampholyte-based capillary electrophoresis (CABCE) has recently been introduced as an alternative to CE (CZE) in the classical buffers. In this study, isoelectric BGEs were obtained by fractionation of Servalyt pH 4-9 carrier ampholytes to cuts of typical width of 0.2 pH unit. CABCE feasibility was examined on a series of insect oostatic peptides, i.e. proline-rich di- to decapeptides, and phosphinic pseudopeptides--tetrapeptide mimetics synthesized as a mixture of four diastereomers having the -P(O)(OH)-CH(2)- moiety embedded into the peptide backbone. With identical selectivity, the separation efficiency of CABCE proved to be as good as classical CE for the insect oostatic peptides and better for diastereomers of the phosphinic pseudopeptides. In addition, despite the numerous species present in the narrow pH cuts of carrier ampholytes, CABCE seems to be free of system zones that could hamper the analysis. Peak symmetry was good for moderately to low mobile peptides, whereas some peak distortion due to electromigration dispersion, was observed for short peptides of rather high mobility.
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Dusan
D
Koval
Jean-Marc
JM
Busnel
Jan
J
Hlavácek
Jirí
J
Jirácek
Václav
V
Kasicka
Gabriel
G
Peltre
D000665:Ampholyte Mixtures / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification*; D010721:Phosphinic Acids / Q000737:chemistry
D000665:Ampholyte Mixtures; D010455:Peptides; D010721:Phosphinic Acids