[Studies of betuionic acid on cell cycle and related protein expressions on mice of bearing H22 tumor cells].
OBJECTIVE
To study the betulonic acid on the cell cycle and related protein expressions on mice of bearing H22 tumor cells.


METHODS
Flow cytometray was used to observe the changes of betulonic acid on the cell cycle and P53 of H22 tumor cells. Immunohistochemistry was determined the espressions of PI3K and AKT.


RESULTS
Increasing the doses of betulonic acid, the number of H22 cells in S phase and G2 phase was increasing gradually, it can speculate that when the betulonic acid act on cells, the cells were blocked in S and G2 phase and inhibited the protein expressions of PI3K and AKT.


CONCLUSIONS
Betulonic acid may be up-regulate the activity of P53 and inhibite the expressions of PI3K and AKT, so that it inhibited the survival pathway of tumor cells.
Zhang
Xiu-juan
XJ
Center of Reserch & Development on Life Science and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China. zxj1717@yahoo.com.cn
Han
Lei
L
Ji
Yu-bin
YB
Fang
Gui-zhen
GZ
D000818:Animals; D029662:Betula / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D016159:Tumor Suppressor Protein p53; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt