[Role of silencing PRL-3 expression by miRNA interference in the growth of gastric cancer].
OBJECTIVE
To investigate the role of silencing PRL-3 expression by miRNA interference in gastric cancer growth.


METHODS
RNA interference mediated by recombinant lentivirus expressing artificial PRL-3 miRNA was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay and tumor implantation experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and the tumor growth.


RESULTS
Transfection of recombinant lentivirus expressing artificial PRL-3 miRNA significantly suppressed the proliferation of SGC7901 cells in vitro. The implanted tumor size of the PRL-3 transfection group was (1.92 +/- 0.18) cm3, significantly smaller than those in control groups [(4.74 +/- 0.39) cm3] (P < 0.05).


CONCLUSIONS
Silencing of PRL-3 significantly suppressed the proliferation of SGC7901 cells and tumor growth in vivo. PRL-3 could be a potential therapeutic target in gastric cancer.
Cai
Shi-rong
SR
Department of Gastrointestinal Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Wang
Zhao
Z
Chen
Chuang-qi
CQ
Cui
Ji
J
Zhang
Chang-hua
CH
He
Yu-long
YL
Zhan
Wen-hua
WH
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D034622:RNA Interference; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
D035683:MicroRNAs; D009363:Neoplasm Proteins; D017027:Protein Tyrosine Phosphatases