Aquasomes--a nanoparticulate approach for the delivery of antigen.
The development of compound that enhances immune responses to recombinant or synthetic epitopes is of considerable importance in vaccine research. Of the many different types of immunopotentiating compounds that have been researched, aquasomes are of considerable promise, because of their potency and adjuvanticity. Aquasomes were prepared by self-assembling of hydroxyapatite by co-precipitation method and thereafter preliminary coated with polyhydroxyl oligomers (cellobiose and trehalose) and subsequently adsorbed with bovine serum albumin (BSA) as a model antigen. The prepared systems were characterized for size, shape, antigen-loading efficiency, in vitro antigen stability, and in vivo performance. BSA-immobilized aquasomes were around 200 nm in diameter and spherical in shape and had approximately 20-30% BSA-loading efficiency. The immunological activity of the formulated aquasomes was compared with plain BSA and better results were observed. Studies also indicated that aquasome formulations could elicit combined T-helper 1 (Th1) and Th2 immune response.
Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. Harisingh Gour Vishwavidyalaya, Sagar, Madhya Pradesh, India.
Amit K
AK
Goyal
Kapil
K
Khatri
Neeraj
N
Mishra
Abhinav
A
Mehta
Bhuvaneshwar
B
Vaidya
Shailja
S
Tiwari
Suresh Prasad
SP
Vyas
D000818:Animals; D002475:Cellobiose / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D016503:Drug Delivery Systems; D017886:Durapatite / Q000737:chemistry*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000276:immunology; D014199:Trehalose / Q000737:chemistry*
D002475:Cellobiose; D012710:Serum Albumin, Bovine; D017886:Durapatite; D014199:Trehalose