Cytokine response to Escherichia coli in gnotobiotic pigs.
One-week-old-germ-free pigs were inoculated with 10(8) CFU of E.coli bacteria-either commensal 086 strain or virulent 055 strain for 1 d. Bacteria were counted in the small intestine, mesenteric lymph nodes, blood and lungs. The O55 strain reached higher levels in circulation and lungs. IL-8, IL-10 and TNF-alpha concentrations were determined by ELISA in plasma and intestinal washes . No difference in cytokine levels was found between control germ-free pigs and their counterparts associated with commensal O86 strain in spite of its high concentration in the gut and circulation.
Division of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Czechia. splichal@gnotobio.cz
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Splichal
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Splichalova
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Trebichavsky
D000818:Animals; D016207:Cytokines / Q000032:analysis* / Q000097:blood; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000276:immunology* / Q000382:microbiology; D005856:Germ-Free Life; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D013552:Swine
D016207:Cytokines