Multilocus genetic analysis of Cryptosporidium isolates from Saudi Arabia.
A total of 35 Cryptosporidium positive samples were collected from children in Jeddah city. The samples were microscopically examined with Ziehl Neelsen (ZN) and Auramin phenol (AP) staining methods. Cryptosporidium antigen was detected in the faecal samples by using the Cryptosporidium ELISA kit. Cryptosporidium sp. were identified by targeting an 840 bp of the hyper-variable region of the 18S rRNA gene and about 550 of the first domain (N terminal) of the COWP gene. The sub-genotypic identification of C. parvum and C. hominis isolates was done by targeting the sporozoite antigen gp15/45/60 gene. Four sp. were identified; C. hominis 13/35 (37%), C. parvum 15/35 (42.9%), C. meleagridis 1/35(2.9 %), & C. muris 1/35 (2.9 %). One isolate was a mixed infection of C. parvum & C. hominis.
Department of Medical Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Faten A
FA
Al-Brikan
Hala S
HS
Salem
Nick
N
Beeching
Nabeel
N
Hilal
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D016054:DNA, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D006801:Humans; D010802:Phylogeny; D016053:RNA, Protozoan / Q000032:analysis; D012337:RNA, Ribosomal, 18S / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D012529:Saudi Arabia; D013045:Species Specificity
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S