[Specific aspects of detection of peroxidase isozymes and their genetic control in barley].
Identical specimens were separated by electrophoresis in two gels to detect and fix peroxidase isozymes. Both gels were stained by Coomassie brilliant blue for detecting proteins. One gel was previously incubated for detecting peroxidase activity. The differences in electrophoretic patterns between the gels indicate the zones of peroxidase activity. It has been shown that locus Prx 6H, controlling a low-mobility grain peroxidase (PRX 6H), is localized to barley chromosome 6. Two loci, Alb 4H and Alb 7H, controlling the biosyntheses of water-soluble proteins of barley endosperm, were localized to chromosomes 4 and 7. It has been demonstrated that barley culture is polymorphic at multiple molecular forms of peroxidase.
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Netsvetaev
D032461:Chromosomes, Plant / Q000235:genetics*; D002523:Edible Grain / Q000201:enzymology / Q000235:genetics; D004589:Electrophoresis, Disc / Q000379:methods; D001467:Hordeum / Q000201:enzymology / Q000235:genetics*; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics; D009195:Peroxidase / Q000032:analysis / Q000235:genetics*; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*
D007527:Isoenzymes; D010940:Plant Proteins; D009195:Peroxidase