Identification of key metabolites of tectorigenin in rat urine by HPLC-MS(n).
This is a report about the identification of key metabolites of tectorigenin in rat urine using high-performance liquid chromatography-electrospray ionization ion trap tandem mass spectrometric method (HPLC-ESI-MS(n)). Six healthy rats were administered a single dose (80 mg/kg) of tectorigenin by oral gavage. Urine was sampled for 0-24 h and centrifuged at 12,000 rpm for 10 min to obtain the supernatants, then the supernatants were purified by solid-phase extraction with a C(18) cartridge. The chromatographic separation was carried out on a reversed-phase C(18) column with a gradient elution program whereas acetonitrile-0.1% formic acid water was used as mobile phase. Mass spectra were acquired in negative ionization mode and a data-dependant scan was used for the identification of the key metabolites of tectorigenin in the urine samples. As a result, four phase II metabolites and the parent drug tectorigenin were found and identified in rat urine for the first time.
Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education; Nanjing, 210009, People's Republic of China.
Wei-Dong
WD
Zhang
Xiao-Lin
XL
Yang
Jun
J
Cao
Ping
P
Li
Zhong-Lin
ZL
Yang
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007529:Isoflavones / Q000008:administration & dosage / Q000378:metabolism* / Q000652:urine*; D008297:Male; D053858:Metabolic Networks and Pathways; D051381:Rats; D017207:Rats, Sprague-Dawley; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D007529:Isoflavones; C120039:tectorigenin