Retention of proteins and metalloproteins in open tubular capillary electrochromatography with etched chemically modified columns.
Etched chemically modified capillaries with two different bonded groups (pentyl and octadecyl) are compared for their migration behavior of several common proteins and metalloproteins as well as metalloproteinases. Migration times, efficiency and peak shape are evaluated over the pH range of 2.1-8.1 to determine any effects of the bonded group on the electrochromatographic behavior of these compounds. One goal was to determine if the relative hydrophobicity of the stationary phase has a significant effect on proteins in the open tubular format of capillary electrochromatography as it does in HPLC. Reproducibility of the migration times is also investigated.
Department of Chemistry, San Jose State University, San Jose, CA 95192, USA. pesek@sjsu.edu
Joseph J
JJ
Pesek
Maria T
MT
Matyska
Vasudha
V
Salgotra
D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D019075:Electrophoresis, Capillary; D008667:Metalloproteins / Q000737:chemistry* / Q000302:isolation & purification; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification
D008667:Metalloproteins; D011506:Proteins