Three dimensional morphometric analyses of axon terminals early changes induced by methylmercury intoxication in the adult cat striate cortex.
The aim of the present report is to investigate in detail morphometric changes of axon terminals of area 17 of adult cat induced by methylmercury intoxication. Six adult male cats (Felix catus), with 12 h day-light cycle and ad libitum water and food regimen, received a single dose of MeHgCl (6.4 mg/kg) dissolved in milk, whereas control subjects (n=6) received only milk. After 30 days, biocytin iontophoretic injections were done into the area 17, (Horsley-Clark coordinates between AP 3.0-6.0) on the crown of the lateral gyrus, near the border with area 18. MeHg and inorganic Hg (Hgi) concentrations were measured in the brain parenchyma of intoxicated cats and corresponded on average to 1.39+/-0.3 and 6.79+/-0.6 ppm (mean+/-s.e.m.) respectively. Twenty four hours after iontophoresis, aldehyde fixed brain sections (200 microm thick), were processed to reveal biocytin labeled terminals. Axonal microscopic 3D reconstructions using Neurolucida software (Microbright Systems Inc.) allowed estimations of boutons, branching points and segment densities for each terminal. Cluster analysis of morphometric axonal features from control and intoxicated group revealed, two distinct axon families (Type I and II) as described elsewhere. Total density values of boutons, branching points and segment densities of intoxicated group, decreased 81, 59 and 91% respectively, as compared to the control group (ANOVA two-way, Bonferroni a priori test p<0.05). Altered axonal morphology associated with MeHg, appeared early in the disease (30 days after contamination), revealing new aspects of the neuronal pathology of the methylmercury intoxication in the visual cortex.
Laboratório de Bioprospecção e Biologia Experimental, Campus de Santarém, Universidade Federal do Pará, Brazil. rbo@ufpa.br
Ricardo Bezerra
RB
de Oliveira
Wallace
W
Gomes Leal
Domingos Luis Wanderley
DL
Picanço-Diniz
João Bento
JB
Torres Neto
Nara
N
Lins
Olaf
O
Malm
Cristovam W
CW
Picanço-Diniz
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D002415:Cats; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D008767:Methylmercury Compounds / Q000008:administration & dosage / Q000633:toxicity*; D018613:Microscopy, Confocal; D017729:Presynaptic Terminals / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors; D014793:Visual Cortex / Q000187:drug effects* / Q000473:pathology
D008767:Methylmercury Compounds; C004925:methylmercuric chloride