[Relationship between soil fungistasis and bacterial community structure].
As a natural attribute of clean and healthy soil, fungistasis is an important indicator of soil quality, and has positive ecological significance to the inhibition of plant disease eruption caused by soil-borne fungal pathogens. In this study, soil samples (0-15 cm) were collected from the abandoned land at Shenyang Experimental Station of Ecology, Chinese Academy of Sciences, to which pesticides and fertilizers have never been applied for nearly 10 years. A series of soil samples with gradient fungistasis was obtained by heating (CK, 100 degrees C, 110 degrees C, and 121 degrees C for 4 min, respectively), and bacterial community structure was analyzed by Polymerase Chain Reaction coupled with Denaturing Gradient Gel Electrophoresis method (PCR-GGGE). The results showed that there was a significant correlation between soil fungistasis and bacterial community composition. Treatment CK showed the strongest capacity to control the growth of target soil-borne pathogenic fungi. The further the bacterial community structure of treated soil deviated from that of CK, the lower the soil fungistasis was. Sequencing and the following phylogenetic analysis of special bands in DGGE indicated that Sphingomonas asaccharolytica, Nitrospira sp., Hyphomicrobiaceae sp., Bacillus megaterium, and Micrococcus sp. could be involved in soil fungistasis.
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. happy_minzi@163.com
Min-Na
MN
Wu
Hui-Wen
HW
Zhang
Xin-Yu
XY
Li
Yan
Y
Zhang
Zhen-Cheng
ZC
Su
Cheng-Gang
CG
Zhang
D000898:Antibiosis / Q000187:drug effects / Q000502:physiology; D000935:Antifungal Agents / Q000032:analysis* / Q000494:pharmacology; D001419:Bacteria / Q000254:growth & development*; D005658:Fungi / Q000187:drug effects / Q000254:growth & development*; D041921:Hyphomicrobiaceae / Q000254:growth & development; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D020578:Sphingomonas / Q000254:growth & development
D000935:Antifungal Agents; D012987:Soil