Simultaneous determination of three carbapenem antibiotics in plasma by HPLC with ultraviolet detection.
A simple, precise and accurate high-performance liquid chromatography (HPLC) method using ultraviolet (UV) detection has been developed for simultaneous determination of carbapenem antibiotics: imipenem, meropenem and ertapenem in human plasma. Samples were spiked with ceftazidime as internal standard and proteins were precipitated by acetonitrile. Separation was achieved on a C8 column with a mobile phase composed of phosphate buffer 0.1M (pH 6.8) and methanol in gradient elution mode. Detection was performed at 298 nm. Calibration curves were linear from 0.5 to 80 mg/L for each compound, with correlation coefficients over 0.997. Intra- and inter-day validation studies showed accuracy between -4.5 and 8.1% and precision below 10.4%. Mean recoveries were 82.2, 90.8 and 87.7% for imipenem, meropenem and ertapenem, respectively. This method provides a useful tool for the therapeutic drug monitoring of carbapenems.
Université Paris Descartes, Inserm, U663, Service de Pharmacologie Clinique, Groupe Hospitalier Cochin-Saint-Vincent de Paul, AP-HP, 75014 Paris, France.
Tiphaine
T
Legrand
Stéphanie
S
Chhun
Elisabeth
E
Rey
Benoît
B
Blanchet
Jean-Ralph
JR
Zahar
Fanny
F
Lanternier
Gérard
G
Pons
Vincent
V
Jullien
D000900:Anti-Bacterial Agents / Q000097:blood; D015780:Carbapenems / Q000097:blood*; D002442:Ceftazidime / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D000077727:Ertapenem; D006801:Humans; D015378:Imipenem / Q000097:blood; D000077731:Meropenem; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013845:Thienamycins / Q000097:blood; D047090:beta-Lactams / Q000097:blood
D000900:Anti-Bacterial Agents; D015780:Carbapenems; D013845:Thienamycins; D047090:beta-Lactams; D015378:Imipenem; D002442:Ceftazidime; D000077731:Meropenem; D000077727:Ertapenem