Toluene and P-xylene mixture exerts antagonistic effect on lipid peroxidation in vitro.
OBJECTIVE
Previous research on a group of workers occupationally exposed to styrene, ethylene glycol, toluene, p-xylene and their mixture showed elevated levels of the main products of lipid peroxidation: malondialdehyde and 4-hydroxynonenal (MDA+,4-HNE) in plasma [1]. Moreover, an earlier in vitro study indicated a synergistic interaction between styrene and ethylene glycol on lipid peroxidation [2]. Therefore, it seemed interesting to investigate the effect of combined exposure to toluene and p-xylene on lipid peroxidation and define the type of the interaction.


METHODS
An in vitro model of human placenta mitochondria was used in the study. The concentration of TBARS (thiobarbituric active reagent species) was measured by spectrophotometry, and of hydroxyl radical (.OH) by assessment of deoxyribose degradation. It was investigated whether the administration of coenzyme Q10 (CoQ10) could have a protective function (if given before solvent exposure) or a reparatory function (if given after exposure) in solvent-induced oxidative stress.


RESULTS
Exposure to p-xylene at concentrations ranging from 5.3 to 265 microg/ml produced an increase in TBARS concentration. The results showed that p-xylene had a stronger influence on lipid peroxidation than toluene. The mixture of toluene and p-xylene induced an antagonistic effect on lipid peroxidation, measured as TBARS concentration. The mechanism connected with .OH generation was found to play an important role in the oxidative damage to lipids resulting from p-xylene exposure. Administration of coenzyme Q10 at the doses of 3.0 and 12.0 microg/ml successfully decreased the TBARS level that was elevated after solvent exposure.


CONCLUSIONS
In contrast to the synergistic effect that the mixture of styrene and ethylene glycol had on lipid peroxidation (previous study), an antagonism between toluene and p-xylene could be observed. The coenzyme Q10 can be considered a protective agent against lipid peroxidation.
Department of Toxicology, Wrocław Medical University, Wrocław, Poland. esawicka@tox.am.wroc.pl
Ewa
E
Sawicka
Anna
A
Długosz
D004357:Drug Synergism; D005260:Female; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010920:Placenta / Q000187:drug effects / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014050:Toluene / Q000494:pharmacology*; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000494:pharmacology; D014992:Xylenes / Q000494:pharmacology*
D017392:Thiobarbituric Acid Reactive Substances; D014992:Xylenes; D014451:Ubiquinone; D017665:Hydroxyl Radical; D014050:Toluene; C024989:coenzyme Q10