[The influence of lens and vitreous resection on anterior chamber associated immune deviation in mice model].
OBJECTIVE
To explore the impact of lens and vitreous resection on the induction of the anterior chamber associated immune deviation (ACAID) in the mice, and its relationship with the time duration postoperatively.


METHODS
The current study was an experimental research. Fifty-one Wistar mice were chosen to involve this study. After the surgeries, 50 Wistar mice were randomly divided into 5 groups. Other 5 SD mice and 1 Wistar mouse were used as the providers of epithelium removed corneal grafts to induce ACAID. Group A: corneal grafts from wistar mouse were implanted into the anterior chamber 1 week after the lens and vitreous resection. Group B: the spleen cells were injected into the nape of neck 1 week after the lens and vitreous resection. The corneal grafts from SD mice were implanted into the anterior chamber 1 week, 4 week and 8 week after the lens and vitreous resection in group C, D and E respectively. To induce the delayed type hypersensitivity (DTH), spleen cells were injected into the right ear pinna 1 week after the neck injection in group B, 2 weeks after the corneal grafts anterior chamber implantation in other groups. At the same time point as the DTH inducement, aqueous humor was collected to measure the concentration of transforming growth factor (TGF) -beta2 and interleukin (IL)-10; heart blood was collected to detect the concentration of IL-4 and IL-10. And the spleens were removed to evaluate the expression of the GATA-3 mRNA by RT-PCR. The eyeballs were enucleated to evaluate the histopathologic changes.


RESULTS
(1) The evaluation of DTH. The DTH were found in group B, C, D and E. (2) The analysis of the TGF-beta2 and IL-10 concentrations in aqueous humor and the IL-4, IL-10 concentrations in serum. The concentrations of IL-4 in serum were (4.073 +/- 0.198) ng/L in group A, (5.806 +/- 0.635) ng/L in group B, (5.535 +/- 0.278) ng/L in group C, (4.102 +/- 0.344) ng/L in group D and (5.313 +/- 0.317) ng/L in group E. The concentrations of IL-10 in serum in group A to group E were (7.854 +/- 2.349) ng/L, (25.633 +/- 6.307) ng/L, (40.103 +/- 16.010) ng/L, (14.321 +/- 2.983) ng/L and (28.620 +/- 5.251) ng/L. The IL-10 concentrations in the aqueous humor were (8.857 +/- 0.401) ng/L, (22.882 +/- 3.315) ng/L, (21.548 +/- 0.477) ng/L, (7.742 +/- 0.952) ng/L and (12.119 +/- 0.477) ng/L, respectively. The concentrations of TGF-beta in the aqueous humor were (5.800 +/- 2.899) ng/L in group A, (60.010 +/- 0.000) ng/L in group B, (57.055 +/- 4.179) ng/L in group C, (28.490 +/- 4.144) ng/L in group D and (36.370 +/- 3.169) ng/L in group E. Significant differences were found in concentrations of TGF-beta2 and IL-10 in aqueous humor and the concentrations of IL-4 and IL-10 in serum in group C, D and E. The concentrations of TGF-beta2, IL-4 and IL-10 were significantly lower in group D than those in group C and E. The trend that the concentrations of TGF-beta2, IL-4 and IL-10 increased after one week postoperatively, then decreased, and finally increased again was observed. (3) The expression of GATA-3 mRNA in spleen. The expression values of GATA-3 mRNA were 662.5 +/- 114.4 in group A, 730.7 +/- 53.8 in group B, 881.9 +/- 10.7 in group C, 1288.3 +/- 258.0 in group D and 1129.7 +/- 95.7 in group E. An obvious up-regulation of the GATA-3 mRNA was noted in group D and E.


CONCLUSIONS
The eye after the lens and vitreous resection lost the ability to induce ACAID in the research duration of this study. But with the gradually decrease of the intraocular inflammation, the ability to induce ACAID might recover with the time.
Department of Ophthalmology, Eye, Ear, Nose and Throat Hospital, Shanghai 200031, China.
Jian-jiang
JJ
Xu
Xing-huai
XH
Sun
Qi-hua
QH
Le
Yan
Y
Wang
Wen-tao
WT
Wang
Tian-yu
TY
Zheng
Jia-xu
JX
Hong
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007908:Lens, Crystalline / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014821:Vitrectomy