Impact of surgical peritoneal environment on postoperative tumor growth and dissemination in a preimplanted tumor model.
BACKGROUND
We recently demonstrated that CO(2) pneumoperitoneum at low intraperitoneal pressure (IPP) had few if any short-term effects on peritoneal dissemination when an ovarian cancer cell line was inoculated just prior to surgery. The objective of the present study was to evaluate the impact of surgical peritoneal environment on postoperative tumor growth and dissemination over time when tumors were present before surgery.


METHODS
On day-7, C57BJ6 mice received an intraperitoneal inoculation of a mouse ovarian cancer cell line (ID8). On day 0, mice were randomized into four groups: anesthesia alone, CO(2) pneumoperitoneum at a low (2 mmHg) or high (8 mmHg) IPP, or laparotomy. Groups were further subdivided into four groups of eight animals each and a laparotomy was performed to evaluate dissemination on postoperative day (POD) 1, 2, 7 or 14.


RESULTS
Peritoneal dissemination score was significantly higher in the laparotomy group compared with in the remaining three groups on PODs 2 and 7. We detected no significant differences in the peritoneal dissemination scores among the low-IPP, high-IPP, and anesthesia groups on PODs 2 and 7. However, there were no significant differences in the peritoneal dissemination score among the three surgical groups on POD 14. Histopathological examination demonstrated that the incidence of invasion of cancer cells into the muscle layers was significantly higher in the laparotomy group than in the low-IPP and anesthesia groups on POD 14. There were no significant differences in tumor growth among the four groups.


CONCLUSIONS
The present findings suggest that CO(2) pneumoperitoneum at either high or low IPP has few if any short-term effects on peritoneal dissemination when tumors are well established before surgery.
Centre d'Endoscopie et des Nouvelles Techniques Interventionnelles, Université d'Auvergne-Clermont I, Clermont-Ferrand, France.
Anne-Sophie
AS
Azuar
Sachiko
S
Matsuzaki
Claude
C
Darcha
Pierre J
PJ
Déchelotte
Jean-Luc
JL
Pouly
Gérard
G
Mage
Michel
M
Canis
D034861:Abdominal Wall / Q000473:pathology; D000818:Animals; D002245:Carbon Dioxide / Q000008:administration & dosage; D045744:Cell Line, Tumor / Q000637:transplantation; D003964:Diaphragm / Q000473:pathology; D005260:Female; D007813:Laparotomy / Q000009:adverse effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D009361:Neoplasm Invasiveness; D009366:Neoplasm Seeding; D010051:Ovarian Neoplasms / Q000473:pathology; D010529:Peritoneal Cavity; D010534:Peritoneal Neoplasms / Q000473:pathology / Q000556:secondary*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects* / Q000379:methods; D011312:Pressure; D011897:Random Allocation
D002245:Carbon Dioxide