The housekeeping gene (GA3PDH) and the long interspersed nuclear element (LINE) in the blood and organs of rats treated with cocaine.
Housekeeping genes are necessary for maintenance of the vital activity of the cells of any phylum of organisms. Transposons or mobile genetic elements are eurysynusic in nature. Thus, the role of these and other genes in the pathogenesis of many diseases and of drug addiction in particular is being investigated. The goal of the work is to determine the influence of cocaine on the activity of GA3PDH and on a representative of the LINE family (L1Rn) in plasma, and in a pellet of blood cells, and in the organs of rats. Gene expression was evaluated by RT-PCR. The GA3PDH (452-bp fragment) was predictably found in plasma, in a pellet of blood cells, and in organs. Its quantity in plasma was greater in the experimental groups than in the control. In a pellet of blood cells and in organs, the GA3PDH activity between the different groups of animals essentially did not differ. The L1Rn fragment (319 bp) in plasma was not found. The expression of L1Rn was much higher in a pellet of blood cells and in organs of experimental animals. These experiments have shown the presence of GA3PDH in the plasma of the controls and an increase in quantity in the plasma of experimental animals. The activation of LINE in a pellet of blood cells of rats and in organs under the influence of cocaine has been demonstrated. Apparently, a recruitment phenomenon of housekeeping genes and transposons is possible in the pathogenesis of drug addiction.
Albert Einstein College Cancer Center, and Department of Oncology, Montefiore Medical Center, Bronx, New York, USA. anatoliyv@yahoo.com
Anatoliy M
AM
Voskresenskiy
Lena S
LS
Sun
D000818:Animals; D000831:Animals, Newborn; D003042:Cocaine / Q000008:administration & dosage / Q000378:metabolism*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000097:blood / Q000235:genetics; D006801:Humans; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D003042:Cocaine