Layer-by-layer buildup of poly(L-glutamic acid)/chitosan film for biologically active coating.
A new biocompatible film based on chitosan and poly(L-glutamic acid) (CS/PGA), created by alternate deposition of CS and PGA, was investigated. FT-IR spectroscopy, UV-vis spectroscopy and QCM were used to analyze the build-up process. The growth of CS and PGA deposition are both exponential to the deposition steps at first. After about 9 (CS/PGA) depositions, the exponential to linear transition takes place. QCM measurements combined with UV-vis spectra revealed the increase in the multilayer film growth at different pH (4.4, 5.0 and 5.5). The build-up of the multilayer stops after a few depositions at pH = 6.5. A muscle myoblast cell (C2C12) assay showed that (CS/PGA)(n) multilayer films obviously promote C2C12 attachment and growth.
Department of Polymer Materials, Shanghai University, 20 Chengzhong Street, Jiading, Shanghai 201800, China.
Zhijiang
Z
Song
Jingbo
J
Yin
Kun
K
Luo
Yanzhen
Y
Zheng
Yan
Y
Yang
Qiong
Q
Li
Shifeng
S
Yan
Xuesi
X
Chen
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D051379:Mice; D032446:Myoblasts / Q000166:cytology*; D011099:Polyglutamic Acid / Q000737:chemistry*; D013057:Spectrum Analysis; D013499:Surface Properties
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D011099:Polyglutamic Acid; D048271:Chitosan