Lymphatic delivery of 99mTc-labeled dextran acetate particles including cyclosporine A.
Biodistribution and lymphoscintigraphy of cyclosporine A (CyA) and technetium-99m (99mTc) were studied using 99mTc-labeled dextran acetate (DxA) including CyA. DxA particles were prepared from dextran with acetic anhydride, and CyA was loaded into them. Lymphatic delivery of 99mTc-labeled DxA particles containing CyA was evaluated after subcutaneous injection into the foot pad of rats and compared with those of 99mTc-labeled human serum albumin (HSA). The labeling efficiency of CyA-loaded 99mTc-DxA particles was about 95% at 30 min. The labeling efficiency maintained stably above 80% for 12 h. The percent injected dose (%ID) of CyA-loaded 99mTc-DxA was similar to that of 99mTc-HSA at the inguinal lymph node after 40 min. The CyA-loaded 99mTc-DxA could be as well distributed as 99mTc-HSA through the lymph node. The DxA particles could steadily distribute the CyA as well as the 99mTc radiolabeling through the lymph node.
Department of Advanced Chemicals Graduate School, Chonnam National University, Gwangju 500-757, Korea.
Jin
J
Kim
Kyong-Hwan
KH
Chung
Chang-Moon
CM
Lee
Young-Soon
YS
Seo
Ho-Chun
HC
Song
Ki-Young
KY
Lee
D000818:Animals; D016572:Cyclosporine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D003911:Dextrans / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems / Q000379:methods; D004355:Drug Stability; D005260:Female; D042601:Lymphatic Vessels / Q000000981:diagnostic imaging / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D011877:Radionuclide Imaging / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000008:administration & dosage / Q000493:pharmacokinetics; D017550:Spectroscopy, Fourier Transform Infrared; D013667:Technetium / Q000493:pharmacokinetics*; D013668:Technetium Tc 99m Aggregated Albumin / Q000493:pharmacokinetics; D014018:Tissue Distribution
D003911:Dextrans; D012709:Serum Albumin; D013668:Technetium Tc 99m Aggregated Albumin; D013667:Technetium; D016572:Cyclosporine