[Detecting hematolysis of Enterococcus from sheep].
OBJECTIVE
To characterize hematolysis of Enterococcus from sheep.


METHODS
Using plate assay (PA), contact hemolysis (CH), supernatant assay (SA), culture hemolysis (CLH) and PCR, we studied hemolysis characteristics of 11 Enterococcus clinical isolates, 30 isolates from healthy sheep, a standard G-Streptococcus and a standard Enterococcus.


RESULTS
Rabbit blood and sheep blood were not hemolysising in the 11 clinical isolates analyzed by SA and CH. Of the clinical isolates 63.6% had beta-hemolysis with rabbit blood and 36.4% had ALPHA-hemolysis with sheep blood analyzed by PA and CLH assay. Of the cylA gene 63.6% was detected in clinical isolates, the sequence of cylA gene was 99.3% homologous with cylA of plasmid pAD1 (GenBank accession number: L37110). J-hemolysis had 53.3% in rabbit blood, alpha-hemolysis and beta-hemolysis in sheep blood had 53.3% and 43.3% respectively in 30 healthy sheep initial isolates with PA. Only 6% had hemolytic capacity in rabbit blood after second generations. The cylA gene was not detected in 30 healthy sheep isolates by PCR. Standard Enterococcus strain of alpha-hemolysis of sheep blood had no hemolysis of rabbit blood.


CONCLUSIONS
The red blood cells could induce enterococci hemolysis secreting in the bacteria growth. The result was different with the Phenotype and the Genotype.
College ofAnimal Science and technology, Shihezi University, Shihezi 832003, China. klc003@163.com
Lichao
L
Kang
Sujuan
S
Han
Jingmei
J
Wang
Xinwen
X
Bo
Xun
X
Ma
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D016983:Enterococcus / Q000235:genetics / Q000276:immunology*; D006461:Hemolysis; D011817:Rabbits; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000382:microbiology*; D013045:Species Specificity
D001426:Bacterial Proteins