Screening of medicinal plants from Reunion Island for antimalarial and cytotoxic activity.
OBJECTIVE
Nine plants from Reunion Island, selected using ethnopharmacology and chemotaxonomy, were investigated for their potential antimalarial value.


METHODS
Thirty-eight extracts were prepared by maceration using CH(2)Cl(2) and MeOH, and were tested for in vitro activity against the 3D7 and W2 strain of Plasmodium falciparum. The most active extracts were then tested for in vitro cytotoxicity on human WI-38 fibroblasts to determine the selectivity index. Those extracts were also investigated in vivo against Plasmodium berghei infected mice.


RESULTS
Most active of the extracts tested were the dichloromethane leaves extracts of Nuxia verticillata Lam. (Buddlejaceae), Psiadia arguta Voigt. (Asteraceae), Lantana camara L. (Verbenaceae), the methanol extracts from Aphloia theiformis (Vahl) Benn. (Aphloiaceae) bark, and Terminalia bentzoe L. (Combretaceae) leaves displaying in vitro IC(50) values ranging from 5.7 to 14.1mug/ml. Extracts from Psiadia, Aphloia at 200mg/(kgday) and Teminalia at 50mg/(kgday) also exhibited significant (p<0.0005) parasite inhibition in mice: 75.5%, 65.6% and 83.5%, respectively.


CONCLUSIONS
Two plants showed interesting antimalarial activity with good selectivity: Aphloia theiformis and Terminalia bentzoe. Nuxia verticillata still needs to be tested in vivo, with a new batch of plant material.
Université de Liège, CIRM, Laboratoire de Pharmacognosie (B36), Av de l'Hôpital, 1, 4000 Liège, Belgium. MC.Jonville@ulg.ac.be
M C
MC
Jonville
H
H
Kodja
L
L
Humeau
J
J
Fournel
P
P
De Mol
M
M
Cao
L
L
Angenot
M
M
Frédérich
D000818:Animals; D000962:Antimalarials / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D002478:Cells, Cultured; D002738:Chloroquine; D004353:Drug Evaluation, Preclinical; D004352:Drug Resistance, Microbial; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry; D008288:Malaria / Q000188:drug therapy*; D051379:Mice; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry*; D010962:Plasmodium berghei / Q000187:drug effects*; D010963:Plasmodium falciparum / Q000187:drug effects*; D011897:Random Allocation; D017508:Reunion
D000962:Antimalarials; D010936:Plant Extracts; D002738:Chloroquine