A bioconjugated polyglycerol dendrimer with glucose sensing properties.
In this work, the biological and electrochemical properties of glucose biosensor based on polyglycerol dendrimer (PGLD) is presented. Streptokinase (SK), glucose oxidase (GOx) and phosphorylcholine (PC) were immobilized onto PGLD to obtain a blood compatible bioconjugate with glucose sensing properties. The bioconjugated PGLD was entrapped in polyaniline nanotubes (PANINT's) through template electrochemical polymerization of aniline. PANINT's were used as electron mediator due to their high ability to promote electron-transfer reactions involving GOx. Platelet adhesion, fibrinolytic activity and protein adsorption were studied by in vitro experiments to examine the interaction of blood with PGLD biosensor. The PGLD biosensor exhibits a strong and stable amperometric response to glucose. The enzyme affinity for the substrate (K (M) (app) ) indicates that the enzyme activity was not significantly altered after the bioconjugation of GOx with PGLD dendrimer. The bioelectrochemical properties suggest that the bioconjugated PGLD developed in this work appears to be a good candidate for providing interfaces for implantable biosensors, especially oxidoreductase-based sensors.
Departamento de Física e Química, Instituto de Ciências Exatas, Universidade Federal de Itajubá (UNIFEI), Av. BPS, 1303, Bairro Pinheirinho, CEP 37500-903 Itajubá, MG, Brazil.
Edson Giuliani Ramos
EG
Fernandes
Alvaro Antonio Alencar
AA
De Queiroz
D015374:Biosensing Techniques / Q000379:methods*; D001774:Blood Chemical Analysis / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D050091:Dendrimers / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005949:Glucose Oxidase / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013300:Streptokinase / Q000737:chemistry*
D001786:Blood Glucose; D050091:Dendrimers; D004800:Enzymes, Immobilized; D011108:Polymers; C043941:polyglycerol; D005949:Glucose Oxidase; D013300:Streptokinase; D005990:Glycerol