Use of flow cytometry to monitor Legionella viability.
Legionella viability was monitored during heat shock treatment at 70 degrees C by a flow cytometric assay (FCA). After 30 min of treatment, for 6 of the 12 strains tested, the FCA still detected 10 to 25% of cells that were viable but nonculturable (VBNC). These VBNC cells were able to produce ATP and to be resuscitated after culture on amoebae.
Groupe Immunité des Muqueuses et Agents Pathogènes, EA3064, Faculté de Médecine J. Lisfranc, Université Jean Monnet, Saint-Etienne, France.
Séverine
S
Allegra
Françoise
F
Berger
Philippe
P
Berthelot
Florence
F
Grattard
Bruno
B
Pozzetto
Serge
S
Riffard
D000656:Amoeba / Q000382:microbiology; D000818:Animals; D005434:Flow Cytometry / Q000379:methods*; D006358:Hot Temperature; D007875:Legionella / Q000254:growth & development / Q000502:physiology* / Q000528:radiation effects; D050296:Microbial Viability