Deglycosylation altered the gating properties of rNav1.3: glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel.
OBJECTIVE
To examine the effect of deglycosylation on gating properties of rNav1.3.


METHODS
rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control.


RESULTS
Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV.


CONCLUSIONS
Glycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.
School of Life Science, Shanghai University, Shanghai 200444, China.
Qing
Q
Xu
Hui-Wen
HW
Cheng
Hui-Qiong
HQ
He
Zhi-Rui
ZR
Liu
Ming
M
He
Hong-Tian
HT
Yang
Zhi-Lei
ZL
Zhou
Yong-Hua
YH
Ji
D000818:Animals; D004553:Electric Conductivity; D004558:Electric Stimulation; D018014:Gene Transfer Techniques; D006031:Glycosylation / Q000187:drug effects; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology*; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D062552:NAV1.3 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins / Q000502:physiology*; D009865:Oocytes; D018408:Patch-Clamp Techniques; D015222:Sodium Channels / Q000502:physiology*; D055672:Static Electricity; D014415:Tunicamycin / Q000494:pharmacology; D014981:Xenopus
D062552:NAV1.3 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins; C478638:Scn3a protein, rat; D015222:Sodium Channels; D014415:Tunicamycin