[The effect of portal blood stasis on lung and renal injury induced by hepatic ischemia reperfusion in a rabbit model].
OBJECTIVE
To investigate the effect and mechanism of portal blood stasis on lung and renal injury induced by hepatic ischemia reperfusion.


METHODS
A rabbit hepatic ischemia reperfusion injury model was established by hepatic portal occlusion and in situ hypothermic irrigation for 30 min. Twenty-four New Zealand white rabbits were employed and randomly divided into 3 groups equally by different dosage of portal blood stasis removal: group A5 (5 ml blood removal), group A10 (10 ml blood removal),and group B (no blood removal). Eight rabbits were served as controls with no hepatic portal occlusion and hypothermic irrigation. After reperfusion 4 h serum endotoxin content, tumor necrosis factor-alpha (TNF-alpha), urea nitrogen (BUN), and creatinine (Cr) were examined respectively, meantime lung and kidney tissues were sampled to determine the content of malondialdehyde (MDA), superoxide dismutase (SOD), the pathology, and wet to dry weight ratio, broncho-alveolar lavage fluid protein content in lung tissues.


RESULTS
Removing portal blood stasis ameliorated lung and renal injury as shown by decreasing the level of serum endotoxin, TNF-alpha, BUN, Cr, wet to dry weight ratio, broncho-alveolar lavage fluid protein content, MDA, SOD. TNF-alpha, Cr, broncho-alveolar lavage fluid protein content in lung tissues and MDA in kidney tissue in group A5 were significantly reduced compared with those in group B (P < 0.05), while in lung tissue in group A10 were also markedly reduced (P < 0.05). The activation of SOD in group A5 were significantly increased (P < 0.05).


CONCLUSIONS
Removal of portal blood stasis before the resume of splanchnic circulation may ameliorate the lung and renal injury induced by hepatic ischemia reperfusion. The possible mechanism may be that portal blood stasis removal reduces endotoxin absorption, and further decreases production of serum TNF-alpha.
Wang
Ye
Y
Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China. qdwangye@163.com
Yang
Jia-mei
JM
Hou
Yuan-kai
YK
Li
Dian-qi
DQ
Hu
Ming-hua
MH
Liu
Peng
P
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007511:Ischemia / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D008099:Liver / Q000098:blood supply; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011169:Portal Vein / Q000473:pathology*; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*