Self-assembled monolayer for low density lipoprotein detection.
Heparin (HEP) has been covalently immobilized onto 4-aminothiophenol (ATP) self-assembled monolayer (SAM) deposited onto gold (Au)-coated glass plate for low density lipoprotein (LDL) detection. The HEP/ATP/Au and LDL/HEP/ATP/Au electrodes have been characterized using cyclic voltammetry (CV) and scanning electron microscopy (SEM). Surface plasmon resonance (SPR) measurements reveal that HEP/ATP/Au electrode is sensitive to detection of the LDL in the range 0.03 microM (10 mg/dl)-0.39 microM (130 mg/dl). The values of association and dissociation rate constants in the association phase calculated by kinetic analysis have been found to be k(a) = 9.67 x 10(1) M(-1) s(-1) and k(d) = 2.64 x 10(-4) s(-1).
Matharu
Zimple
Z
Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, New Delhi-110012, India.
Arya
Sunil K
SK
Sumana
G
G
Gupta
Vinay
V
Malhotra
B D
BD
D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006493:Heparin / Q000737:chemistry; D008077:Lipoproteins, LDL / Q000032:analysis* / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D020349:Surface Plasmon Resonance
D008077:Lipoproteins, LDL; D006046:Gold; D006493:Heparin