Putting the model to the test: are APC proteins essential for neuronal polarity, axon outgrowth, and axon targeting?
The highly polarized architecture of neurons is important for their function. Experimental data based on dominant-negative approaches suggest that the tumor suppressor adenomatous polyposis coli (APC), a regulator of Wnt signaling and the cytoskeleton, regulates polarity of neuroectodermal precursors and neurons, helping specify one neurite as the axon, promoting its outgrowth, and guiding axon pathfinding. However, such dominant-negative approaches might affect processes in which APC is not essential. We completely removed both APCs from Drosophila melanogaster larval neural precursors and neurons, testing whether APCs play universal roles in neuronal polarity. Surprisingly, APCs are not essential for asymmetric cell division or the stereotyped division axis of central brain (CB) neuroblasts, although they do affect cell cycle progression and spindle architecture. Likewise, CB, lobular plug, and mushroom body neurons do not require APCs for polarization, axon outgrowth, or, in the latter two cases, axon targeting. These data suggest that proposed cytoskeletal roles for APCs in mammals should be reassessed using loss of function tools.
Rusan
Nasser M
NM
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Akong
Kathryn
K
Peifer
Mark
M
D025601:Adenomatous Polyposis Coli Protein / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000502:physiology; D001921:Brain / Q000166:cytology; D002455:Cell Division; D016764:Cell Polarity; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism; D007814:Larva / Q000166:cytology; D008954:Models, Biological; D024521:Mushroom Bodies / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D008941:Spindle Apparatus / Q000378:metabolism
D025601:Adenomatous Polyposis Coli Protein