The adhesion and spreading of thrombocyte vesicles on electrode surfaces.
The interaction of thrombocyte vesicles with the surface of metal electrodes, i.e., mercury, gold and gold electrodes modified with self assembled monolayers (SAM), was studied with the help of chronoamperometry, atomic force microscopy, and quartz crystal microbalance measurements. The experimental results show that the interaction of the thrombocyte vesicles with the surface of the electrodes depends on the hydrophobicity of the latter: whereas on very hydrophobic surfaces (mercury and gold functionalized with SAM) the thrombocyte vesicles disintegrate and form a monolayer of lipids, on the less hydrophobic gold surface a bilayer is formed. The chronoamperometric measurements indicate the possibility of future applications to probe membrane properties of thrombocytes.
Institut für Biochemie, Universität Greifswald, Felix-Hausdorff-Str. 4, D-17487, Greifswald, Germany.
Victor
V
Agmo Hernández
Juliane
J
Niessen
Falk
F
Harnisch
Stephan
S
Block
Andreas
A
Greinacher
Heyo K
HK
Kroemer
Christiane A
CA
Helm
Fritz
F
Scholz
D000268:Adhesiveness; D001792:Blood Platelets / Q000166:cytology*; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008670:Metals; D018625:Microscopy, Atomic Force
D008670:Metals