Bioinformatics analysis of metastasis-related proteins in hepatocellular carcinoma.
OBJECTIVE
To analyze the metastasis-related proteins in hepatocellular carcinoma (HCC) and discover the biomarker candidates for diagnosis and therapeutic intervention of HCC metastasis with bioinformatics tools.


METHODS
Metastasis-related proteins were determined by stable isotope labeling and MS analysis and analyzed with bioinformatics resources, including Phobius, Kyoto encyclopedia of genes and genomes (KEGG), online mendelian inheritance in man (OMIM) and human protein reference database (HPRD).


RESULTS
All the metastasis-related proteins were linked to 83 pathways in KEGG, including MAPK and p53 signal pathways. Protein-protein interaction network showed that all the metastasis-related proteins were categorized into 19 function groups, including cell cycle, apoptosis and signal transduction. OMIM analysis linked these proteins to 186 OMIM entries.


CONCLUSIONS
Metastasis-related proteins provide HCC cells with biological advantages in cell proliferation, migration and angiogenesis, and facilitate metastasis of HCC cells. The bird's eye view can reveal a global characteristic of metastasis-related proteins and many differentially expressed proteins can be identified as candidates for diagnosis and treatment of HCC.
Institute of Biomedical Science, Fudan University, Shanghai 200320, China.
Pei-Ming
PM
Song
Yang
Y
Zhang
Yu-Fei
YF
He
Hui-Min
HM
Bao
Jian-Hua
JH
Luo
Yin-Kun
YK
Liu
Peng-Yuan
PY
Yang
Xian
X
Chen
D014408:Biomarkers, Tumor / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000737:chemistry* / Q000473:pathology; D045744:Cell Line, Tumor; D019295:Computational Biology; D030541:Databases, Genetic; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry* / Q000473:pathology; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis*; D011379:Prognosis; D025941:Protein Interaction Mapping; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins