Embryonic development and metamorphosis of the scyphozoan Aurelia.
We investigated the development of Aurelia (Cnidaria, Scyphozoa) during embryogenesis and metamorphosis into a polyp, using antibody markers combined with confocal and transmission electron microscopy. Early embryos form actively proliferating coeloblastulae. Invagination is observed during gastrulation. In the planula, (1) the ectoderm is pseudostratified with densely packed nuclei arranged in a superficial and a deep stratum, (2) the aboral pole consists of elongated ectodermal cells with basally located nuclei forming an apical organ, which is previously only known from anthozoan planulae, (3) endodermal cells are large and highly vacuolated, and (4) FMRFamide-immunoreactive nerve cells are found exclusively in the ectoderm of the aboral region. During metamorphosis into a polyp, cells in the planula endoderm, but not in the ectoderm, become strongly caspase 3 immunoreactive, suggesting that the planula endoderm, in part or in its entirety, undergoes apoptosis during metamorphosis. The polyp endoderm seems to be derived from the planula ectoderm in Aurelia, implicating the occurrence of "secondary" gastrulation during early metamorphosis.
Department of Ecology and Evolutionary Biology, UCLA, Los Angeles, CA 90095-1606, USA.
David
D
Yuan
Nagayasu
N
Nakanishi
David K
DK
Jacobs
Volker
V
Hartenstein
D000818:Animals; D036703:Blastula / Q000166:cytology / Q000648:ultrastructure; D049109:Cell Proliferation; D047108:Embryonic Development / Q000502:physiology*; D054262:Gastrulation; D007814:Larva / Q000166:cytology / Q000648:ultrastructure; D008675:Metamorphosis, Biological / Q000502:physiology*; D008954:Models, Biological; D037401:Scyphozoa / Q000166:cytology / Q000196:embryology* / Q000254:growth & development / Q000648:ultrastructure; D013270:Stomach / Q000196:embryology