Nonclassical SNAPFL analogue as a Cy5 resonance energy transfer partner.
We have synthesized a new SNAPFL analogue (1) that exhibits a large Stokes shift (78 nm) (abs. 542 nm, em. 620 nm) and a good quantum yield. Because of the large overlap between the emission spectrum of 1 and the absorption spectrum of Cy5, 1 functions well as a fluorescence donor to Cy5 and has been used in FRET-based experiments using estrogen receptor site-specifically labeled with Cy5 and a receptor ligand conjugated to SNAPFL.
Department of Chemistry of University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
Sung Hoon
SH
Kim
Jillian R
JR
Gunther
John A
JA
Katzenellenbogen
D001565:Benzoates / Q000737:chemistry* / Q000145:classification; D002232:Carbocyanines / Q000737:chemistry*; D003506:Cyclofenil / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D014966:Xanthenes / Q000737:chemistry* / Q000145:classification
C533355:(3,10-dihydroxybenz(c)xanthene-7-yl)-4-carboxy-2-methylbenzene; D001565:Benzoates; D002232:Carbocyanines; D014966:Xanthenes; C085321:cyanine dye 5; D003506:Cyclofenil