A novel transgenic mice model for venous malformation.
Vascular anomalies are most commonly seen in the head and neck region, and there is no animal model available of this disease until now. The purpose of this study was to construct a conditional murine polyomavirus middle T antigen gene (PyMT) transgenic mice model, in order to provide a basis for the treatment of vascular anomalies in vivo, as well as the study of PyMT's molecular function. A new conditional transgenic vector based on Tet-On system was constructed successfully. After the experiment in vitro, pronuclear microinjection method was used to introduce the purified transgene into the chromosomes of fertilized mice eggs, and five transgenic positive mice were obtained. The transgenic positive animals went down to future generation by hybridization. After induction of PyMT's expression in the F1 generation, three transgenic mice developed venous malformation which was confirmed histopathologically. The mice model generated could be used as a tool to study venous malformation, as well as the function of PyMT gene.
Wang
Yan An
YA
Department of Oral & Maxillofacial Surgery, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
Zheng
Jia Wei
JW
Fei
Zhao Liang
ZL
Jiang
Xin Quan
XQ
Wang
Zhu Gang
ZG
Fei
Jian
J
Zhang
Zhi Yuan
ZY
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D019556:COS Cells; D002522:Chlorocebus aethiops; D002875:Chromosomes; D004195:Disease Models, Animal; D005821:Genetic Techniques; D005822:Genetic Vectors; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008957:Models, Genetic; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D019076:Transgenes; D054079:Vascular Malformations / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming