[Study of gastric carcinoma and PCNA and c-met gene abnormality].
OBJECTIVE
To explore the changed regularity of proliferating cell nuclear antigen (PCNA) and c-met gene abnormality in the oncogenic pathway of gastric carcinoma.


METHODS
Semi-quantitative PCR was used to detect the amplification of c-met gene in 58 gastric carcinoma tissues and corresponding para-carcinoma tissues. Immunohistochemical staining was used to detect the expression and over expression of c-met and PCNA protein.


RESULTS
The amplification of c-met was detected in 58 cases was significant statistic difference on the amplification of c-met oncogene between the two groups (t = 9.455, P < 0.01). The positive rate of c-met protein in gastric carcinoma tissues was 67.24% (39/58), which was negative in the corresponding para-carcinoma tissues. There was significant difference between the two groups (chi2 = 58.75, P < 0.001). PCNA LI of 58 gastric carcinoma tissues and the corresponding adjacent tissues were 49.3768 +/- 12.1832 and 14.8390 +/- 7.1847, respectively. The PCNA LI had a significant difference between the two groups( t = 8.805, P < 0.01) . The amplification and overexpression of c-met gene in gastric carcinoma tissues has no relationship with the tumor's size, location, lymph node metastasis, differentiation degree and deeper invasion. The amplification of c-met oncogene was related with the oncogenesis of gastric carcinoma.


CONCLUSIONS
The abnormal expression of c-met and PCNA protein was related with the oncogenesis of gastric carcinoma.
Ji
Jing
J
School of Public Health, Qingdao University, Qingdao 266021, China. jijing2001319@163.com
Zhao
Peng
P
Huang
Baohua
B
D005260:Female; D005784:Gene Amplification / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism*; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
D018809:Proliferating Cell Nuclear Antigen; D019859:Proto-Oncogene Proteins c-met