[Effect of cryopreservation of bone marrow stromal cells on periodontal tissue regeneration].
OBJECTIVE
To investigate the influence of cryopreservation on bone marrow stromal cells' (BMSC) capability of enhancing periodontal regeneration.


METHODS
Twenty-six artificial periodontal defects were established in 5 Beagle dogs and divided into 3 groups at random. Only collagen membrane, the complex of cryopreserved and un-cryopreserved BMSC and collagen scaffold were transplanted in the blank control group, cryopreserved and un-cryopreserved groups respectively. The periodontal regeneration was observed 8 weeks after transplantation.


RESULTS
The percentage of periodontal regeneration in cryopreserved and un-cryopreserved groups was significantly greater than that of the blank control group (P < 0.05), but no statistical difference was found between cryopreserved and un-cryopreserved groups.


CONCLUSIONS
Cryopreservation had no significant negative effects on BMSC capability of enhancing periodontal regeneration.
Department of Periodontology, School of Stomatology, Fujian Medical University, Fuzhou 350002, China.
Hou-Xuan
HX
Li
Fu-Hu
FH
Yan
Lang
L
Lei
Jian-Guo
JG
Liu
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D015925:Cryopreservation; D004285:Dogs; D005260:Female; D016557:Guided Tissue Regeneration, Periodontal; D010510:Periodontal Diseases / Q000601:surgery*; D014182:Transplantation, Autologous