Variation in gene expression of inflammatory cytokines in leukocyte-derived cells of high-fat-diet-induced insulin-resistant rats.
A high-fat diet is thought to enhance inflammation in various tissues by increasing insulin resistance. In this study, we determined the mRNA levels of inflammatory cytokines in leukocyte-derived cells in the blood of rats with high-fat-diet-induced insulin resistance. Feeding rats a high-fat diet for 77 d induced moderate insulin resistance, which was determined by increased plasma glucose and insulin concentrations, following an oral glucose tolerance test. The interleukin (IL)-1beta mRNA level was higher in the insulin-resistant rats than in control rats at the fasting stage, whereas the tumor necrosis factor (TNF)-alpha mRNA level was greatly elevated at 180 min after glucose administration in the insulin-resistant rats. The results suggest that feeding rats a high-fat diet enhances the expression of fasting IL-1beta and postprandial TNF-alpha genes in leukocyte-derived cells.
Laboratory of Nutritional Physiology, Graduate School of Nutritional and Environmental Sciences, Global COE Program, The University of Shizuoka, Shizuoka, Japan.
Saki
S
Fujimoto
Kazuki
K
Mochizuki
Masaya
M
Shimada
Yuki
Y
Murayama
Toshinao
T
Goda
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D016207:Cytokines / Q000235:genetics*; D005223:Fats / Q000378:metabolism / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics*; D007333:Insulin Resistance / Q000235:genetics*; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D016207:Cytokines; D005223:Fats; D012333:RNA, Messenger