Degradation of sphingoid long-chain base 1-phosphates (LCB-1Ps): functional characterization and expression of AtDPL1 encoding LCB-1P lyase involved in the dehydration stress response in Arabidopsis.
Sphingoid long-chain base (LCB) 1-phosphates are degradated by LCB 1-phosphate lyase to C(16) fatty aldehydes and phosphoethanolamine. Here, we confirmed that the At1g27980 gene product, AtDPL1, is a functional LCB-1-phosphate lyase. Expression of green fluorescent protein fusion products in suspension-cultured Arabidopsis cells showed that AtDPL1 is located to the endoplasmic reticulum. The rates of fresh weight decreases of dpl1-1 and dpl1-2 mutants were significantly slower than those of the wild-type plants. This ability to limit their transpiration reflected the leaf temperature of the mutant plants more than that of wild-type plants, suggesting that AtDPL1 plays a role in dehydration stress.
Nishikawa
Masahiro
M
Department of Biology, Graduate School of Natural Science, Konan University, Kobe, 658-8501, Japan.
Hosokawa
Kenta
K
Ishiguro
Mai
M
Minamioka
Hiroki
H
Tamura
Kentaro
K
Hara-Nishimura
Ikuko
I
Takahashi
Yohei
Y
Shimazaki
Ken-ichiro
K
Imai
Hiroyuki
H
D000446:Aldehyde-Lyases / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D003681:Dehydration / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009154:Mutation; D010710:Phosphates / Q000378:metabolism*; D018526:Plant Transpiration; D018749:RNA, Plant / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D013312:Stress, Physiological
D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D010710:Phosphates; D018749:RNA, Plant; C036483:T-DNA; D049452:Green Fluorescent Proteins; D000446:Aldehyde-Lyases; C022649:sphingosine 1-phosphate lyase (aldolase)