Brucella abortus strain RB51 leucine auxotroph as an environmentally safe vaccine for plasmid maintenance and antigen overexpression.
To avoid potentiating the spread of an antibiotic resistance marker, a plasmid expressing a leuB gene and a heterologous antigen, green fluorescent protein (GFP), was shown to complement a leucine auxotroph of cattle vaccine strain Brucella abortus RB51, which protected CD1 mice from virulent B. abortus 2308 and elicited GFP antibodies.
Rajasekaran
Parthiban
P
Department of Biomedical Sciences and Pathobiology and Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0342, USA.
Seleem
Mohamed N
MN
Contreras
Andrea
A
Purwantini
Endang
E
Schurig
Gerhardt G
GG
Sriranganathan
Nammalwar
N
Boyle
Stephen M
SM
D000818:Animals; D002004:Brucella Vaccine / Q000235:genetics* / Q000276:immunology*; D002003:Brucella abortus / Q000235:genetics* / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control*; D018628:Gene Dosage; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D007930:Leucine / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D010957:Plasmids
D002004:Brucella Vaccine; D049452:Green Fluorescent Proteins; D007930:Leucine