Transthyretin knockout mouse nerves have increased lipoprotein lipase and sphingolipid content following crush.
Transthyretin (TTR) knockout (KO) mice display increased levels of lipoprotein lipase (LPL) and impaired nerve regeneration. Given LPL potential role in the reutilization of myelin lipids following injury, we compared myelin lipid content in wild-type and TTR KO mice after nerve crush. We found that LPL is expressed not only in Schwann cells but also in dorsal root ganglia neurons and that its activity is increased in TTR KO mice following nerve injury. As a possible consequence of LPL increase in the regenerating nerve of TTR KOs, the sphingolipids sphingomyelin and galactocerebroside were augmented in the distal nerve stump. Given their ability to increase neurite outgrowth, upregulation of LPLand sphingolipids in a system with decreased capacity for nerve regeneration probably constitutes a compensatory mechanism.
Nerve Regeneration Group, IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua Campo Alegre 823, 4150-180 Porto, Portugal.
Ana Filipa
AF
Nunes
Mónica Mendes
MM
Sousa
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005699:Galactosylceramides / Q000378:metabolism; D005727:Ganglia, Spinal / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009186:Myelin Sheath / Q000378:metabolism; D009416:Nerve Regeneration / Q000235:genetics*; D011228:Prealbumin / Q000235:genetics*; D012583:Schwann Cells / Q000378:metabolism; D020426:Sciatic Neuropathy / Q000235:genetics* / Q000378:metabolism* / Q000503:physiopathology; D011984:Sensory Receptor Cells / Q000378:metabolism; D013107:Sphingolipids / Q000378:metabolism*; D013109:Sphingomyelins / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology
D005699:Galactosylceramides; D008563:Membrane Lipids; D011228:Prealbumin; D013107:Sphingolipids; D013109:Sphingomyelins; D008071:Lipoprotein Lipase