Expression and sequence variation of the cucumber Por gene in the synthesized allotetraploid Cucumis x hytivus.
The molecular evolution of the cucumber Por gene in early generations of the synthesized allotetraploid Cucumis x hytivus was investigated. The results from gene expression analysis showed that the cucumber Por gene was silenced in the S(1) generation, and re-activated in the S(2) generation. In the S(3) and S(4) generations, the transcripts remained activated but sequence changes were observed. Further analysis indicated that base substitutions, including two transitions and one transversion, occurred in the S(1) and S(3) generation, respectively, and in the S(3) generation, an intron was found to be retained in the transcript. This indicates allopolyploidy induced rapid silencing and mutation of the cucumber Por gene. Further, gene mutations such as base substitution and intron retention are modes of evolution for duplicated genes in newly formed polyploids.
Zhuang
Yong
Y
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Chen
Jin-Feng
JF
Jahn
Molly
M
D001483:Base Sequence; D003433:Crosses, Genetic; D028462:Cucumis / Q000235:genetics*; D004171:Diploidy; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006824:Hybridization, Genetic; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011123:Polyploidy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger