Specific detection of Xanthomonas oryzae pv. oryzicola in infected rice plant by use of PCR assay targeting a membrane fusion protein gene.
Successful control of Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak, requires a specific and reliable diagnostic tool. A pathovar-specific PCR assay was developed for the rapid and accurate detection of the plant pathogenic bacterium Xanthomonas oryzae pv. oryzicola in diseased plant. Based on differences in a membrane fusion protein gene of Xanthomonas oryzae pv. oryzicola and other microorganisms, which was generated from NCBI (http://www.ncbi.nlm.nih.gov/) and CMR (http://cmr.tigr.org/) BLAST searches, one pair of pathovar-specific primers, XOCMF/XOCMR, was synthesized. Primers XOCMF and XOCMR from a membrane fusion protein gene were used to amplify a 488-bp DNA fragment. The PCR product was only produced from 4 isolates of Xanthomonas oryzae pv. oryzicola among 37 isolates of other pathovars and species of Xanthomonas, Pectobacterium, Pseudomonas, Burkholderia, Escherichia coli, and Fusarium oxysporum f.sp. dianthi. The results suggested that the assay detected the pathogen more rapidly and accurately than standard isolation methods.
National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea.
Man Jung
MJ
Kang
Jae Kyung
JK
Shim
Min Seok
MS
Cho
Young Joo
YJ
Seol
Jang Ho
JH
Hahn
Duk Ju
DJ
Hwang
Dong Suk
DS
Park
D001426:Bacterial Proteins / Q000235:genetics; D030541:Databases, Genetic; D005798:Genes, Bacterial; D050576:Membrane Fusion Proteins / Q000235:genetics*; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000382:microbiology; D014974:Xanthomonas / Q000235:genetics* / Q000302:isolation & purification*
D001426:Bacterial Proteins; D050576:Membrane Fusion Proteins