CD8+ suppressor and cytotoxic T cells recognize the same human leukocyte antigen-A2 restricted cytomegalovirus peptide.
We explored the possibility that antigen-specific human CD8(+) T cells, which display cytotoxic or suppressor function, can recognize the same peptide epitope. Using the human leukocyte antigen-A0201 restricted immunodominant cytomegalovirus epitope pp65-NLVPMVATV for pulsing either mature/immunogenic or ILT3(high)ILT4(high) tolerogenic dendritic cells (DC), we generated cytotoxic and suppressor CD8(+) T-cell lines, respectively. Our data indicate that modulating the functional state of DC is crucial to the development of tolerogenic or immunogeneic peptide-specific vaccines.
Department of Pathology, Columbia University, New York, NY 10032, USA.
Haiyan
H
Qin
George
G
Vlad
Raffaello
R
Cortesini
Nicole
N
Suciu-Foca
John S
JS
Manavalan
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology*; D022482:Cytomegalovirus Vaccines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology*; D015789:HLA-A2 Antigen; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D010750:Phosphoproteins / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology*
D022482:Cytomegalovirus Vaccines; D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; C491341:LILRB2 protein, human; C107907:LILRB4 protein, human; D008562:Membrane Glycoproteins; D010455:Peptides; D010750:Phosphoproteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa