[Induced pluripotent stem cells generated from reprogramming differentiated cells by defined factors].
Embryonic stem cell is promising for regenerative medicine. However, its application is hampered by the utilization of eggs in most established methods. Recently, a new pluripotent stem cell establishing method was reported that, mouse and human differentiated cells could be induced reprogrammed into a pluripotent state by expressing exogenetic stem factors such as Oct4, Sox2, et al, through retroviral transduction. This approach avoiding egg use is a great breakthrough not only in stem cell technology but also present theory hypothesis of reprogramming. Here these works were reviewed in this article. Both the mechanism of induced reprogramming and the prospects of induced pluripotent stem cells were discussed.
Key Laboratory of Developmental Biology, Xinhua Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200092, China.
Xiaoyu
X
Xia
Jianxin
J
Chu
Xuejin
X
Chen
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology*; D012190:Retroviridae / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000378:metabolism*; D014161:Transduction, Genetic
D050814:Octamer Transcription Factor-3; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse