[Study on absorption kinetics of astragaloside IV in rats intestines].
OBJECTIVE
To investigate the mechanisms of intestine absorption of astragaloside IV in rat.


METHODS
The K(a) and P(app) of astragaloside IV was investigated using single-pass intestinal perfusion technique in rats. HPLC was used to determine the concentration of astragaloside IV. The effect of absorption site, drug concentration and the inhibitors of P-glycoproteon on the absorption had been studied.


RESULTS
By the testing of the statistics, the K(a) and the P(app) values of the duodenum, jejunum, ileum, colonic had significant differences (P < 0.05). The concentration from 20-80 mg x L(-1) had no distinctive effect on the K(a) and P(app) of small intestine. The inhibitors of P-glycoproteon had no distinctive effect on the absorption of small intestine.


CONCLUSIONS
Astragaloside IV is absorbed by typical passive diffusion mechanism.
Huang
Huai-Peng
HP
Hebei Ping'an Healthy Group Co. Ltd., Shijiazhuang 050021, China. huang71526@163.com
Liu
Cai-Xia
CX
Li
Yan-Ling
YL
Gao
Guo-Ling
GL
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000378:metabolism / Q000493:pharmacokinetics*; D014315:Triterpenes / Q000378:metabolism / Q000493:pharmacokinetics*; D014700:Verapamil / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A; D014700:Verapamil