From defined reactive diblock copolymers to functional HPMA-based self-assembled nanoaggregates.
This paper describes the synthesis of functional amphiphilic poly( N-(2-hydroxypropyl) methacrylamide)-block-poly(lauryl methacrylate) copolymers by RAFT polymerization via the intermediate step of activated ester block copolymers (pentafluoro-phenyl methacrylate). Block copolymers with molecular weights from 12000-28000 g/mol and PDIs of about 1.2 have been obtained. The amphiphilic diblock copolymers form stable super structures (nanoaggregates) by self-organization in aqueous solution. The diameters of these particles are between 100 and 200 nm and depend directly on the molecular weight of the block copolymer. Furthermore, we investigated the impact of these nanoaggregates on cell viability and on the motility of adherent cells. Cytotoxicity was investigated by the MTS test and the fluctuation in cell shape was monitored employing ECIS (electrical cell-substrate impedance sensing). In these investigations, the formed particles are not cell toxic up to a concentration of 2 mg/mL. Thus, our polymeric particles offer potential as polymer therapeutics.
Institute of Organic Chemistry, Johannes Gutenberg-University Mainz, Duesbergweg 10-14, 55099 Mainz, Germany.
M
M
Barz
M
M
Tarantola
K
K
Fischer
M
M
Schmidt
R
R
Luxenhofer
A
A
Janshoff
P
P
Theato
R
R
Zentel
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002460:Cell Line; D002465:Cell Movement; D048430:Cell Shape; D002470:Cell Survival; D004285:Dogs; D007850:Lauric Acids / Q000737:chemistry*; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D008970:Molecular Weight; D053758:Nanoparticles; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology
D001672:Biocompatible Materials; D007850:Lauric Acids; D008689:Methacrylates; D011108:Polymers; C421257:poly(lauryl methacrylate); C032802:hydroxypropyl methacrylate