[Study on spectral and immune identification of artificial antigen of bisphenol A].
Bisphenol A(BPA) is able to mimic the effects of endogenous hormones and it influences the central nervous system in human being. In recent years, BPA has largely arisen because it is released into our environment from many kinds of the polycarbonate plastics and epoxy resins of food cans. Because trace residual amount of BPA might be harmful to human being, many instrument analysis methods have been developed for the determination of BPA. But these instrument analysis methods require complicated pre-treatment steps and do not suit rapid processing of multiple samples. Gold-labeled immunochromatography assay method can provide in situ monitoring of trace BPA in ground-water and drinking water. In order to build the simple and fast gold-labeled immunochromatography assay method, it is necessary to synthesize and identify artificial antigen of BPA firstly. The modified BPA was conjugated with the carrier of bovine serum albumin (BSA) by EDC * HCl, after dialyzed, the synthesized artificial antigen of BPA was lyophilized, and then stored at -20 degrees C. Three methods such as UV and IR spectra scanning and immunoassay were used to determine whether artificial antigen of BPA had been synthesized. The results showed that UV spectrum of artificial antigen of BPA has one absorbance band at 278 nanometers (nm) which is the characteristic absorbance band of the carrier of bovine serum albumin, and another absorbance band at 226 nm which is the characteristic absorbance band of BPA. The IR spectrum absorbance bands of bovine serum albumin and BPA appeared on the IR spectrum graph of artificial antigen of BPA, moreover, shrinked vibration bands at 1 000-1 300 cm(-1) showed that a C-N bond was produced and suggested that the modified BPA and BSA had been linked. The antibody of anti-BPA was raised in sera of immunized Balb/c mice by indirect competitive ELISA. So the above results showed that the artificial antigen of BPA was synthesized successfully. The artificial antigen of BPA can be used to develop monoclonal antibody of anti-BPA and also to prepare gold-labeled immunochromatography assay method for in situ monitoring of BPA in the future.
Zheng
Jie
J
Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China.
Zhang
Kun
K
Zhao
Su-Qing
SQ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D001559:Benzhydryl Compounds; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D010636:Phenols / Q000032:analysis* / Q000276:immunology*; D013055:Spectrophotometry, Infrared; D000098826:Bisphenol A Compounds
D000911:Antibodies, Monoclonal; D000941:Antigens; D001559:Benzhydryl Compounds; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds