[Cloning and expression of the pig skeletal muscle musclin gene].
We found seven tag sequence with high homology in dbEST by using human musclin gene, and got its cDNA sequence, which consists of 651bp and the open reading frame was 54-452 bp detected by RT-PCR, encoding 132 amino acid residue protein. The new gene has high homology with that of human, mouse and rat, the rate is 87.2%, 77.6% and 77.9%, respectively; the gene fragment was cloned into expression vector pGEX-4T-1, and the recombinant was transformed into E. coli BL21. Induced by IPTG, the fusion protein GST-musclin, a 38.59 kD protein was successfully expressed in E. coli BL21 and identified by Western blotting.
Wang
Weijie
W
Laboratory ofAnimal Physiology & Biochemistry, He'nan Agricultural University, Zhengzhou 450002, China.
Li
Hongji
H
Han
Liqiang
L
Wang
Yueying
Y
Wang
Jing
J
Li
Weihua
W
Lin
Maowang
M
Tai
Yulei
Y
Zhang
Zhiqiang
Z
Zang
Meng
M
Wang
Yanling
Y
Yang
Guoyu
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D013552:Swine / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics
D018076:DNA, Complementary; D009124:Muscle Proteins; C480236:OSTN protein, human; C498450:Ostn protein, rat; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors