The identification of neurotensin NTS1 receptor partial agonists through a ligand-based virtual screening approach.
We identified small molecule NTS1R agonist compounds through virtual screening of the corporate database using a ROCS approach that searches multi-conformer representations efficiently. As a starting point for the ROCS search, we used the known NTS1R selective antagonist, SR-48527, based on the hypothesis that NT agonists and antagonists might share similar binding regions. Conformations were expanded and selected as database search queries based on a cluster analysis. The search provided us with virtual hits that were tested in intracellular calcium mobilization assays of NTS1R agonist and antagonist activities measured in FLIPR format as well as in [(3)H]NT competition binding studies. The results indicated that two initial hits produced partial agonist activity with potency in the moderate micromolar range.
Chemical & Screening Sciences, Wyeth Research, Princeton, NJ 08543, USA.fank@wyeth.com
Yi
Y
Fan
Margaret H
MH
Lai
Kelly
K
Sullivan
Michael
M
Popiolek
Terrance H
TH
Andree
Paul
P
Dollings
Mark H
MH
Pausch
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D018028:Receptors, Neurotensin / Q000819:agonists*
D008024:Ligands; D018028:Receptors, Neurotensin