Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge.
The taxonomic positions of two environmental isolates from South Korea were established using a combination of genotypic and phenotypic data. The organisms, designated PB314(T) and Ho-08(T), were Gram-negative, rod-shaped and non-spore-forming and had chemotaxonomic properties consistent with their classification in the genus Deinococcus 16S rRNA gene tree, the highest sequence similarities being shown to the type strains of Deinococcus grandis (96.3-96.7 %) and Deinococcus indicus (96.3-96.4 %). The isolates shared relatively high 16S rRNA gene sequence similarity (98.1 %) but had a DNA-DNA relatedness value of only 22 %. Chemotaxonomic data revealed that both strains possess quinone system MK-8 as the predominant compound, C(16 : 1)omega7c and C(16 : 0) as major fatty acids and ornithine as a diamino acid in the peptidoglycan structure, corroborating our assignment of the strains to the genus Deinococcus. The results of phylogenetic analyses based on 16S rRNA gene sequences, DNA-DNA relatedness values and physiological and biochemical tests clearly demonstrated that the two strains represent distinct species. On the basis of these data, two novel species, Deinococcus aquaticus sp. nov. (type strain PB314(T) =KCTC 12552(T) =NBRC 101311(T)) and Deinococcus caeni sp. nov. (type strain Ho-08(T) =KCTC 12553(T) =NBRC 101312(T)), are proposed.
Im
Wan-Taek
WT
Environmental and Molecular Microbiology Lab, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, Republic of Korea. wandra@kaist.ac.kr
Jung
Hae-Min
HM
Ten
Leonid N
LN
Kim
Myung Kyum
MK
Bora
Nagamani
N
Goodfellow
Michael
M
Lim
Sangyong
S
Jung
Jinwoo
J
Lee
Sung-Taik
ST
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D034301:Deinococcus / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000528:radiation effects; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D005720:Gamma Rays; D005798:Genes, Bacterial; D020459:Genes, rRNA; D005838:Genotype; D050296:Microbial Viability; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D012722:Sewage