Determination of estrogens and their metabolites in water using C30 SPE-LC-MS.
Triacontyl bonded silica (C(30)) material was applied as solid-phase extraction (SPE) sorbent and an SPE-LC-MS method was established for the determination of eight estrogens and their metabolites in water samples. Compared to commercial C(18) SPE cartridge, the performance of C(30) was evaluated in various important SPE conditions, such as sorbent mass, elution solvent and its volume, loading flow rate, and sample loading volume. The results showed a superior performance of C(30)-C(18) by the shorter treatment time and fewer required elution solvent. In the optimum conditions, the results showed good recoveries (80.5-109.4%), excellent linear relationships (0.02-1 ng/mL, except 2-MeO-E(2)), high precisions (lower than 10.0% RSD for both low and high spiked concentration), and low LODs (1-16 ng/L). Method validation using C(30) packed cartridge was also testified with spiked real water samples, including tap water and river water. Satisfy results demonstrated the feasibility of C(30) SPE to the analysis for real environmental waters.
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Wei
W
Yan
Lixia
L
Zhao
Qinzhong
Q
Feng
Jin-Ming
JM
Lin
D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D004967:Estrogens / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply
D004967:Estrogens; D014874:Water Pollutants, Chemical