[Effect of cisplatin on the expression of Pokemon gene: experiment with different human lung cancer cells].
OBJECTIVE
To investigate the correlation between Pokemon gene and cisplatin mechanism.


METHODS
Human lung adenocarcinoma cells of the lines A549 and AGZY83-a, human lung squamous carcinoma cells of the line HE-99, and human giant cell lung cancer cells of the line 95D were cultured and cisplatin was added into the medium. Other lung cancer cells of the above mentioned lines were cultured in the medium without cisplatin and were used as control groups. RT-PCR and Western blotting were used to detect the mRNA and protein expression of Pokemon.


RESULTS
Pokemon mRNA and protein were expressed highly in all the 4 cell lines. The Pokemon gene expression did not changed significantly after cisplatin treatment groups. There were not significant differences in the mRNA and protein expression of Pokemon among the 4 experiment groups and the control groups (all P > 0.05).


CONCLUSIONS
Cisplatin has no effect on the Pokemon gene expression of the human lung cancer cells.
Zhao
Zhi-Hong
ZH
Department of Chest Surgery, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. zzh517cs@yahoo.com.cn
Wang
Sheng-Fa
SF
Yu
Liang
L
Wang
Ju
J
Cong
De-Gang
DG
Chang
Hao
H
Wang
Xue-Feng
XF
Zhang
Tie-Wa
TW
Zhang
Jian
J
Fu
Kai
K
Jiang
Jiu-Yang
JY
D000970:Antineoplastic Agents / Q000494:pharmacology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D000970:Antineoplastic Agents; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C485514:ZBTB7A protein, human; D002945:Cisplatin