Estrogen and progesterone receptor isoforms expression in the stomach of Mongolian gerbils.
OBJECTIVE
We studied the estrogen receptor (ER) and progesterone receptor (PR) isoforms expression in gastric antrum and corpus of female gerbils and their regulation by estradiol (E2) and progesterone (P4).


METHODS
Ovariectomized adult female gerbils were subcutaneously treated with E2, and E2 + P4. Uteri and stomachs were removed, the latter were cut along the greater curvature, and antrum and corpus were excised. Proteins were immunoblotted using antibodies that recognize ER-alpha, ER-beta, and PR-A and PR-B receptor isoforms. Tissues from rats treated in the same way were used as controls.


RESULTS
Specific bands were detected for ER-alpha (68 KDa), and PR isoforms (85 and 120 KDa for PR-A and PR-B isoforms, respectively) in uteri, gastric antrum and corpus. We could not detect ER-beta isoform. PR isoforms were not regulated by E2 or P4 in uterus and gastric tissues of gerbils. ER-alpha isoform content was significantly down-regulated by E2 in the corpus, but not affected by hormones in uterus and gastric antrum.


CONCLUSIONS
The presence of ER-alpha and PR isoforms in gerbils stomach suggests that E2 and P4 actions in this organ are in part mediated by their nuclear receptors.
Departamento de Biologia, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Coyoacan, Mexico DF04510, Mexico.
Milena
M
Saqui-Salces
Teresa
T
Neri-Gomez
Armando
A
Gamboa-Dominguez
Guillermo
G
Ruiz-Palacios
Ignacio
I
Camacho-Arroyo
D000818:Animals; D015153:Blotting, Western; D004958:Estradiol / Q000008:administration & dosage / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D005849:Gerbillinae; D007279:Injections, Subcutaneous; D010052:Ovariectomy; D011374:Progesterone / Q000008:administration & dosage / Q000378:metabolism; D020033:Protein Isoforms; D011706:Pyloric Antrum / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011980:Receptors, Progesterone / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D020033:Protein Isoforms; D011980:Receptors, Progesterone; C101118:progesterone receptor A; C097314:progesterone receptor B; D011374:Progesterone; D004958:Estradiol