Alpinia katsumadai Hayata prevents mouse sepsis induced by cecal ligation and puncture through promoting bacterial clearance and downregulating systemic inflammation.
Sepsis continues to be a challenge in clinic. Therapeutic strategies focus on local host defenses and the inhibition of overwhelming inflammation response. The present study aimed to investigate the protective effects and the underlying mechanisms of the ethanol extract of Alpinia katsumadai Hayata seeds (EAKH) on polymicrobial sepsis induced by cecal ligation and puncture (CLP) in mice. It was shown that oral administration of EAKH at 1 h before and 2 h after CLP significantly elevated the survival rate and the mean arterial pressure of mice. Histological examination and serum ALT/AST assessment demonstrated that EAKH protected the animals from lung and liver tissue injury and dysfunction. Although EAKH was devoid of direct bacteriostatic or bacteriocidal activities, it facilitated peritoneal bacteria clearance and increased leukocyte migration into peritoneal cavity of septic mice. Furthermore, EAKH remarkably decreased serum pro-inflammatory cytokine (TNF-alpha, IL-1beta and NO) levels in septic mice. These findings demonstrated that EAKH has preventive effects on mouse sepsis induced by CLP, which may be attributed to elevating local defense via promoting leukocyte migration to infection focus and attenuating systemic inflammation.
Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 1 Shennong Road, Nanjing 210038, China.
Jian
J
Yang
Yue
Y
Dai
Yu-Feng
YF
Xia
Wen-Zhe
WZ
Huang
Zheng-Tao
ZT
Wang
D032429:Alpinia / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000276:immunology / Q000494:pharmacology*; D002432:Cecum / Q000293:injuries; D002465:Cell Movement / Q000276:immunology; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000276:immunology; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D020556:Neutrophil Infiltration / Q000187:drug effects; D009569:Nitric Oxide / Q000276:immunology; D010936:Plant Extracts / Q000276:immunology / Q000494:pharmacology*; D018805:Sepsis / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide