Contributions of different intraarticular tissues to the acute phase elevation of synovial fluid MMP-2 following rat ACL rupture.
Inflammation and accumulation of matrix metalloproteinases (MMPs) in synovial fluids may be involved in the poor healing ability of the anterior cruciate ligament (ACL) after injury. With a rat ACL rotating injury model, we found that levels of IL-1beta, IL-6, and TNF-alpha were significantly higher in synovial fluids after ACL injury. MMP-2 activity and global MMP activity in synovial fluids also increased significantly in a time-dependent manner. Ex vivo studies showed that all tissues contributed to the elevation of MMP-2 in synovial fluids, especially synovium and the injured ACL. We concluded that although the regular wound-healing mechanism also occurs after ACL injury, accumulation of MMP activity in the synovial fluids, due to all of the intraarticular tissues, may be at least one of the important reasons why an injured ACL cannot be repaired.
Tang
Zhenyu
Z
111 Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.
Yang
Li
L
Wang
Yequan
Y
Xue
Ruyue
R
Zhang
Jin
J
Huang
Wei
W
Chen
Peter C
PC
Sung
K-L Paul
KL
D000210:Acute-Phase Reaction / Q000378:metabolism; D000818:Animals; D016118:Anterior Cruciate Ligament / Q000276:immunology / Q000378:metabolism*; D000070598:Anterior Cruciate Ligament Injuries; D002358:Cartilage, Articular / Q000276:immunology / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D008592:Menisci, Tibial / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012421:Rupture / Q000276:immunology / Q000378:metabolism; D013582:Synovial Fluid / Q000276:immunology / Q000378:metabolism*; D013585:Synovitis / Q000276:immunology / Q000378:metabolism*; D046509:Tissue Culture Techniques; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D003470:Culture Media; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D020778:Matrix Metalloproteinase 2