Immunoregulatory effects of ethyl-acetate fraction of extracts from Tetrastigma hemsleyanum Diels et. Gilg on immune functions of ICR mice.
OBJECTIVE
To evaluate the effects of ethyl-acetate fraction (EAF) of extracts from Tetrastigma hemsleyanum Diels et. Gilg (TDG) on immune functions of ICR mice.


METHODS
ICR mice were exposed to different doses of EAF for 15 or 30 days and then their immune functions were analyzed, including ConA-induced splenic lymphocyte transformation, SRBC-induced delayed type hypersensitivity response, serum hemolysin analysis, antibody-producing cells, peritoneal macrophage phagocytized chicken red blood cells, natural killer cell activity, and serum level of cytokines.


RESULTS
EAF of extracts from TDG at different doses had various effects on immune functions of ICR mice. As compared with the controls, it increased the mouse spleen lymphocyte transformation induced by ConA, the left-hind voix pedis thickness and the number of plague forming cells (PFCs) at the dose of 1.82 mg/mL, 5.48 mg/mL, and 9.12 mg/mL, respectively; increased the ink clearance ability at the dose of 0.91 mg/mL, 1.82 mg/mL, 5.48 mg/mL, and 9.12 mg/mL, respectively; increased the phagocytosis index of mononuclear-macrophages and production of serum interferon-gamma (IFN-gamma) at the dose of 5.48 mg/mL; and could promote the production of serum tumor necrosis factor-alpha (TNF-alpha) at the dose of 9.12 mg/mL.


CONCLUSIONS
EAF of extracts from TDG can regulate mouse immune functions in vivo.
Department of Toxicology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310009, Zhejiang, China.
Cai-Ju
CJ
Xu
Gang-Qiang
GQ
Ding
Jian-Yun
JY
Fu
Jia
J
Meng
Rong-Hua
RH
Zhang
Xiao-Ming
XM
Lou
D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D007111:Immunity, Cellular / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D027844:Vitaceae / Q000737:chemistry*
D000085:Acetates; D010936:Plant Extracts; C007650:ethyl acetate