A comparative proteomic analysis during urodele lens regeneration.
To examine underlying mechanisms of urodele lens regeneration we have employed a proteomic analysis of 650 proteins involved in several signaling pathways. We compared expression of these proteins between the regeneration-competent dorsal iris and the regeneration-incompetent ventral iris in the newt. After a series of screenings we selected several proteins to evaluate their expression quantitatively on immunoblots. We then used these selected proteins to compare their expression between the dorsal iris of the newt and the iris of the axolotl, another urodele, which does not regenerate the lens. In the newt we find that most proteins are expressed in both dorsal and ventral iris, even though there is differential regulation. Moreover, several of these proteins are expressed in the axolotl iris as well and for some of them their expression is consistent with the regeneration potential.
Department of Biology and Center for Tissue Regeneration and Engineering, University of Dayton, 300 College Park, Dayton, OH 45469-2320, USA.
Meagan
M
Roddy
Timothy P
TP
Fox
Jonathan P
JP
McFadden
Kenta
K
Nakamura
Katia
K
Del Rio-Tsonis
Panagiotis A
PA
Tsonis
D000558:Ambystoma mexicanum / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D015151:Immunoblotting; D007908:Lens, Crystalline / Q000378:metabolism / Q000502:physiology*; D009673:Notophthalmus viridescens / Q000378:metabolism / Q000502:physiology*; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D012038:Regeneration
D011506:Proteins