Novel analysis of sleep patterns in rats separates periods of vigilance cycling from long-duration wake events.
Rats are polyphasic sleepers. However, a formal definition of when one sleep episode ends and another begins has not been put forth. In the present study we examine the distribution of wake episode durations and based on this distribution conclude there are multiple components of wake. If the wake episode exceeds 300 s the wake episode is assigned to long-duration wake (LDW), if the episode is less than 300 s it is assigned to brief wake (BW). Further support for this separation was found in close analysis of the EEG power spectrum in BW versus LDW. We then used LDW episodes to separate one sleep episode from another. We term the sleep episodes vigilance cycling (VC) because the rat is cycling between the vigilance states of BW, slow-wave sleep (SWS), and rapid-eye movement sleep (REMS). We find that the characteristics of VC are different in the light period versus the dark period. We further find that as VC episodes progress, SWS pressure lessens, but the amount of time spent in REMS increases. These findings suggest that VC episodes are regulated and meaningful to the sleep behavior of rats. The use of the concepts of LDW and VC provides additional insights into the description of sleep patterns in rats that may be important in the development of a complete description of sleep behavior in this animal.
Simasko
Steven M
SM
Program in Neuroscience, Washington State University, Pullman, WA 99164-6520, USA. simasko@vetmed.wsu.edu
Mukherjee
Sanjib
S
D000818:Animals; D001143:Arousal / Q000502:physiology*; D003624:Darkness; D003627:Data Interpretation, Statistical; D004569:Electroencephalography; D004576:Electromyography; D008027:Light; D008297:Male; D010507:Periodicity; D017286:Polysomnography; D051381:Rats; D012890:Sleep / Q000502:physiology*; D012894:Sleep Stages / Q000502:physiology; D012895:Sleep, REM / Q000502:physiology; D014851:Wakefulness / Q000502:physiology*