ERETIC implemented in diffusion-ordered NMR as a diffusion reference.
The ERETIC (Electronic Reference To access In vivo Concentrations) technique generates an electronic signal in the NMR spectrometer which is detected simultaneously to the sample FID during the acquisition. The implementation of the ERETIC sequence in any 2D DOSY experimental scheme enables one to generate directly into the raw 2D DOSY spectrum a reference signal with an attenuation simulated to describe a well-defined diffusion behavior. This simulated intensity attenuation can be used to evaluate the output generated by any DOSY data treatment algorithm, in a single as well as multichannel approach and provide insight into their precision, accuracy, scope and limitations. The ERETIC sequence implemented in the standard bipolar pulsed field gradient longitudinal eddy current delay (LED) sequence is illustrated on various algorithms presented previously in the literature for the analysis of the generated ERETIC-DOSY spectra of simulated model systems representing discrete and continuous diffusion profiles in mono- and bi-Gaussian diffusion regimes.
Van Lokeren
Luk
L
Department of Materials and Chemistry (MACH), High Resolution NMR Centre (HNMR), Vrije Universiteit Brussel, Pleinlaan 2, Brussels, Belgium. lvlokere@vub.ac.be
Kerssebaum
Rainer
R
Willem
Rudolph
R
Denkova
Pavletta
P
D000465:Algorithms; D004058:Diffusion; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D016011:Normal Distribution