CFTR and defective endocytosis: new insights in the renal phenotype of cystic fibrosis.
Inactivation of the chloride channel cystic fibrosis transmembrane conductance regulator (CFTR) causes cystic fibrosis (CF). Although CFTR is expressed in the kidney, no overwhelming renal phenotype is associated with CF. Recent studies have shown that the level of CFTR mRNA in mouse kidney approaches that found in lung. CFTR is particularly abundant in the apical area of proximal tubule cells, where it co-distributes with the Cl(-)/H(+) exchanger ClC-5 and Rab5a in endosomes. The biological relevance of CFTR in proximal tubule endocytosis has been tested in CF mouse models and CF patients. Mice lacking CFTR show a defective receptor-mediated endocytosis, as evidenced by impaired uptake of (125)I-beta(2)-microglobulin, a decreased expression of the cubilin receptor in the kidney, and a significant excretion of cubilin and its low-molecular-weight ligands into the urine. Low-molecular-weight proteinuria (and particularly transferrinuria) is similarly detected in CF patients in comparison with normal controls or patients with chronic lung inflammation. These studies suggest that the functional loss of CFTR impairs the handling of low-molecular-weight proteins by the kidney, supporting a role of CFTR in receptor-mediated endocytosis in proximal tubule cells. The selective proteinuria should be integrated in the pathophysiology of multi-systemic complications increasingly observed in CF patients.
Jouret
François
F
Division of Nephrology, Université catholique de Louvain Medical School, Avenue Hippocrate, 10, 1200 Brussels, Belgium.
Devuyst
Olivier
O
D000818:Animals; D018118:Chloride Channels / Q000502:physiology; D003550:Cystic Fibrosis / Q000503:physiopathology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000502:physiology; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D007668:Kidney / Q000503:physiopathology*; D007687:Kidney Tubules, Proximal / Q000503:physiopathology; D051379:Mice
D018118:Chloride Channels; D019005:Cystic Fibrosis Transmembrane Conductance Regulator