[Differential expression protein between the thuringiensin-yield Bacillus thuringiensis strain CT-43 and its mutants].
OBJECTIVE
The purpose of this research is trying to uncover the biosynthetic or metabolic relative protein of thuringiensis by proteomics.


METHODS
We conducted differential expression analysis between the high thuringiensin-yield Bacillus thuringiensis strain CT-43 and its mutants, high production strain CT-43-1C and non-production strain BMB0806, based on the 2-D gel. Then through mass spectrum (MS) identification and bio-informatics we analyzed the detected proteins.


RESULTS
Thirteen spots were selected to be detected by the MS, and nine of them were identified. Among them, six proteins including in the basal metabolism pathway were possibly deduced that relative with the synthesis or metabolism of thuringiensin production.


CONCLUSIONS
There were six proteins found to be connected with the influential role protein of biosynthesis and metabolic of thuringiensin production by comparative proteomics. This research provides the evidence of thuringiensis gene cluster cloning and biosynthetic analysis.
State Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China. guochl19821218@webmail.hzau.edu.cn
Chengliang
C
Guo
Zhenfei
Z
Hu
Xiaoyan
X
Liu
Ziquan
Z
Yu
Lifang
L
Ruan
Ming
M
Sun
Ziniu
Z
Yu
D000241:Adenosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D053898:Biosynthetic Pathways; D019295:Computational Biology; D030562:Databases, Protein; D015964:Gene Expression Regulation, Bacterial; D013058:Mass Spectrometry; D009154:Mutation; D040901:Proteomics; D013400:Sugar Acids
D001426:Bacterial Proteins; D013400:Sugar Acids; C068295:thuringiensin; D000241:Adenosine