Influence of ethanol on the high frequency electric polarizability of E. coli.
The interface electric polarizability of bacteria (charge dependent (ChD) and Maxwell-Wagner (MW) polarizabilities) gives information about their electric charge, determined by the structure and functional state. It is well known that the polarizability could be changed significantly by adding some substances to the suspension, and can be measured using an electro-optical (EO) method. There are some literature data, according to which the adding of ethanol decreases the electric polarizability of the cells. However the reason for the change in this parameter is not clear, as well as which component (ChD and/or MW) of polarizability has the main contribution. Generally the present work shows that the effect of ethanol is connected to the change of the internal (cytoplasm) MW polarizability and is mainly caused by increasing the cell membrane permeability. This results in an ionic flow through the membrane, which velocity and direction depends on the relative values of the inner (cytoplasm) and the outer medium ionic strength.
Gyurova
Anna Y
AY
Institute of Physical Chemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 11, Sofia 1113, Bulgaria. any_gyurova@abv.bg
Zhivkov
Alexandar M
AM
D000818:Animals; D002463:Cell Membrane Permeability; D016764:Cell Polarity / Q000187:drug effects / Q000528:radiation effects; D004574:Electromagnetic Fields; D004594:Electrophysiology; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D000431:Ethanol / Q000494:pharmacology*; D007700:Kinetics; D009994:Osmolar Concentration
D000431:Ethanol