Far-red fluorescence: a direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenching.
Time-resolved fluorescence on oligomers of the main light-harvesting complex from higher plants indicate that in vitro oligomerization leads to the formation of a weakly coupled inter-trimer chlorophyll-chlorophyll (Chl) exciton state which converts in tens of ps into a state which is spectrally broad and has a strongly far-red enhanced fluorescence spectrum. Both its lifetime and spectrum show striking similarity with a 400ps fluorescence component appearing in intact leaves of Arabidopsis when non-photochemical quenching (NPQ) is induced. The fluorescence components with high far-red/red ratio are thus a characteristic marker for NPQ conditions in vivo. The far-red emitting state is shown to be an emissive Chl-Chl charge transfer state which plays a crucial part in the quenching.
Miloslavina
Yuliya
Y
Max-Planck-Institut für Bioanorganische Chemie, Germany. miloslaw@mpi-muelheim.mpg.de
Wehner
Antje
A
Lambrev
Petar H
PH
Wientjes
Emilie
E
Reus
Michael
M
Garab
Gyozo
G
Croce
Roberta
R
Holzwarth
Alfred R
AR
D001059:Apoproteins / Q000737:chemistry*; D017360:Arabidopsis / Q000737:chemistry*; D015415:Biomarkers / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D005453:Fluorescence; D007700:Kinetics; D010777:Photochemistry; D045332:Photosystem II Protein Complex / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D001059:Apoproteins; D015415:Biomarkers; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D002734:Chlorophyll