Isothermal titration calorimetry of RNA.
Isothermal titration calorimetry (ITC) is a fast and robust method to study the physical basis of molecular interactions. A single well-designed experiment can provide complete thermodynamic characterization of a binding reaction, including K(a), DeltaG, DeltaH, DeltaS and reaction stoichiometry (n). Repeating the experiment at different temperatures allows determination of the heat capacity change (DeltaC(P)) of the interaction. Modern calorimeters are sensitive enough to probe even weak biological interactions making ITC a very popular method among biochemists. Although ITC has been applied to protein studies for many years, it is becoming widely applicable in RNA biochemistry as well, especially in studies which involve RNA folding and RNA interactions with small molecules, proteins and with other RNAs. This review focuses on best practices for planning, designing and executing effective ITC experiments when one or more of the reactants is an RNA.
Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA.
Nilshad N
NN
Salim
Andrew L
AL
Feig
D000465:Algorithms; D002151:Calorimetry / Q000295:instrumentation / Q000379:methods*; D001330:Electronic Data Processing; D009690:Nucleic Acid Conformation; D011485:Protein Binding / Q000235:genetics; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000737:chemistry; D013816:Thermodynamics; D017186:Titrimetry / Q000379:methods
D012330:RNA, Double-Stranded; D012313:RNA