Centrifugation on a single layer of colloid selects improved quality spermatozoa from frozen-thawed stallion semen.
The present study attempted to select the subpopulation of stallion spermatozoa that best survived a conventional freezing and thawing procedure, using centrifugation of post-thawed semen samples through a single layer of a glycidoxypropyltrimethoxysilane-coated silica colloid with a species-specific formulation (Androcoll-E). Sperm motility, sperm chromatin structure, membrane integrity and mitochondrial membrane potential were studied in filtered and non-filtered spermatozoa. Single-layer centrifugation (SLC) using Androcoll-E significantly improved all the sperm parameters studied, implying SLC may be a simple approach to improve the quality of frozen-thawed (FT) spermatozoa for AI.
Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Avda de Universidad s/n, Cáceres, Spain.
B
B
Macías García
J M
JM
Morrell
C
C
Ortega-Ferrusola
L
L
González-Fernández
J A
JA
Tapia
H
H
Rodriguez-Martínez
F J
FJ
Peña
D000818:Animals; D002498:Centrifugation / Q000379:methods*; D003102:Colloids; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D053938:DNA Fragmentation; D005615:Freezing; D006736:Horses / Q000502:physiology*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology
D003102:Colloids