Enzymatic removal of flatulence-inducing sugars in chickpea milk using free and polyvinyl alcohol immobilized alpha-galactosidase from Aspergillus oryzae.
The treatment of chickpea milk was carried out in batch, repeated batch and continuous reaction by soluble and polyvinyl alcohol (PVA) immobilized Aspergillus oryzae alpha-galactosidase for the removal of raffinose family oligosaccharides (RFOs). In the batch mode of treatment 96 and 92% of RFOs hydrolysis was observed by soluble and immobilized enzyme, respectively. In repeated batch experiments, immobilized enzyme showed 70% RFOs hydrolysis up to sixth cycle. Polyvinyl alcohol immobilized alpha-galactosidase in fluidized bed reactor showed highest reduction of 94% at a flow rate of 30 ml/h. The results obtained from the present study are very interesting for industrial use of PVA-immobilized enzyme.
Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka, 585 106, India.
Aravind Goud G
AG
Patil
Naganagoud V
NV
Kote
Veerappa
V
Mulimani
D001236:Aspergillus oryzae / Q000201:enzymology*; D029863:Cicer / Q000737:chemistry / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005414:Flatulence / Q000209:etiology*; D005511:Food Handling / Q000379:methods*; D005656:Fungal Proteins / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011887:Raffinose / Q000737:chemistry / Q000378:metabolism*; D000519:alpha-Galactosidase / Q000378:metabolism*
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011142:Polyvinyl Alcohol; D000519:alpha-Galactosidase; D011887:Raffinose