Association and haplotype analysis of purH gene with inosine monophosphate content in chickens.
The current study was designed to investigate the effects of the purH gene on chicken muscle inosine monophosphate (IMP) content. Muscle IMP content was measured in five chicken breeds. Single nucleotide polymorphisms (SNPs) were detected by PCR-SSCP and DNA sequencing. Two SNPs were detected, A/T substitution at position 8023 in exon 9, and T/C substitution at position 17446 in exon 16. The results indicated that only T17446C polymorphism was associated with IMP content. The haplotype effect was higher than the single genotype effect. We tentatively conclude that purH gene is a candidate locus or linked to a major gene that affects muscle IMP content. Haplotypes are superior to single genotypes as potential molecular markers for meat quality traits in chicken.
Shu
J T
JT
Institute of Poultry Science, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu, P.R. China.
Bao
W B
WB
Zhang
X Y
XY
Zhang
H X
HX
Chen
G H
GH
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D006239:Haplotypes; D019877:Hydroxymethyl and Formyl Transferases / Q000235:genetics* / Q000378:metabolism; D007291:Inosine Monophosphate / Q000378:metabolism*; D016014:Linear Models; D008460:Meat / Q000592:standards*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D009709:Nucleotide Deaminases / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D009097:Multienzyme Complexes; C113120:inosine monophosphate synthase; D007291:Inosine Monophosphate; D012313:RNA; D019877:Hydroxymethyl and Formyl Transferases; D009709:Nucleotide Deaminases