Functionalization of multi-walled carbon nanotubes and their application for selective isolation of acidic proteins.
A micro-column packed with PDDA-wrapped MWNTs in sequential injection system facilitates selective sorption of acidic protein species. Proteins adsorbed onto the PDDA-MWNT composites are afterwards collected by elution with a citrate buffer as stripping reagent. With a sample loading volume of 2.0 mL and an eluent volume of 200 microL, a retention efficiency of 100% and a recovery of 90% are achieved for BSA in the range 6-120 microg, resulting in an enrichment factor of 14. A sampling frequency of 15 h(-1) is achieved, along with a precision of 4.5% at 25 microg x mL(-1) BSA. The practical applicability of this system is demonstrated by processing human whole blood for successive isolation of acidic proteins.
Du
Zhuo
Z
Research Center for Analytical Sciences, Box 332, Northeastern University, Shenyang 110004, China.
Yu
Yong-Liang
YL
Wang
Jian-Hua
JH
D000143:Acids / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification; D002417:Cattle; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D002942:Circular Dichroism; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification
D000143:Acids; D001798:Blood Proteins; D006454:Hemoglobins; D037742:Nanotubes, Carbon; D012710:Serum Albumin, Bovine