[hNUDC promotes proliferation and differentiation of megakaryocytopoiesis on human CD341+ cells].
OBJECTIVE
aTo study the effect of human nuclear distribution C èhNUDCé on human megakaryocyte proliferation and differentiation from cord blood CD34(+) cells in vitro.


METHODS
aHuman CD34(+) cells were isolated using the Dynal CD34 Progenitor Cell Selection System from umbilical cord blood. The CD34(+) cells were then cultured in serum free methylcellulose semi-solid media, the morphologic aspects and number of small, medium and large CFU-MK colonies were observed and scored on the day12 by microscopy analysis. The CD34(+) cells were cultured in serum free liquid media, cells were removed on day 10 and formation of CD41(+) in human megakaryocyte and its DNA polyploidization of nuclear were analyzed on a FACsort flowcytometer.


RESULTS
ahNUDC supported the formation of small and medium CFU-MK colony in serum free semi-solid media. Furthermore, hNUDC induced a remarkable increase in expression of the megakaryocyte cell surface marker CD41(+) and stimulated the CD41(+) DNA polyploidization more effectively than TPO.


CONCLUSIONS
hNUDC may play an important role in megakaryocyte proliferation and differentiation.
First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Yan-Qiu
YQ
Chen
Yi-Chen
YC
Ge
Pei-Lin
PL
Xu
Yan
Y
Yang
D018952:Antigens, CD34 / Q000276:immunology*; D018797:Cell Cycle Proteins / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D009687:Nuclear Proteins / Q000494:pharmacology*; D040201:Platelet Membrane Glycoprotein IIb / Q000235:genetics / Q000276:immunology; D011123:Polyploidy; D034061:Thrombopoiesis / Q000187:drug effects*; D013926:Thrombopoietin / Q000494:pharmacology
D018952:Antigens, CD34; D018797:Cell Cycle Proteins; C512648:NUDC protein, human; D009687:Nuclear Proteins; D040201:Platelet Membrane Glycoprotein IIb; D013926:Thrombopoietin