Coenzyme Q10 diminishes ischemia-reperfusion induced apoptosis and nerve injury in rabbit urinary bladder.
OBJECTIVE
Ischemia/reperfusion (I/R) can significantly change the nerve function of the bladder, thus resulting in detrusor weakness and overactivity. CoQ10 is a lipid-soluble cofactor found naturally in the mitochondria and has been reported to have neuroprotective and antiapoptosis effects. The aim of this study is to determine if CoQ10 can protect bladders subjected to I/R injury.


METHODS
Four groups of male New Zealand White rabbits (N = 4) were treated with CoQ10 (3 mg/kg body weight/day) (groups 1 and 2) or vehicle (groups 3 and 4). In groups 2 and 4 (I/R groups), bilateral vesicular ischemia was induced for 2 hr and the rabbits allowed to recover for 2 weeks. Groups 1 and 3 were controls and given sham surgery. The cholinergic nerve marker, vesicular acetylcholine transporter (VAChT), was examined by western blotting. Nerve density and cell apoptosis were calculated by immunohistochemistry.


RESULTS
I/R significantly decrease bladder innervation; CoQ10 has significant neuroprotective effects, which are evidenced by increased VAChT expression and neurofilament immunostaining. Detrusor cells apoptosis increase significantly by I/R. CoQ10 control and I/R groups both show significantly lower apoptosis than vehicle groups.


CONCLUSIONS
The current study clearly demonstrated that these CoQ10 supplement provides significant bladder protection against I/R injury. This protective effect is in part by protecting damage to cholinergic innervation.
Juan
Yung-Shun
YS
Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan.
Chuang
Shu Mien
SM
Mannikarottu
Anita
A
Huang
Chun-Hsung
CH
Li
Suning
S
Schuler
Catherine
C
Levin
Robert M
RM
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D005260:Female; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D016900:Neurofilament Proteins / Q000378:metabolism; D018696:Neuroprotective Agents; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000187:drug effects / Q000502:physiology; D011817:Rabbits; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000627:therapeutic use; D001743:Urinary Bladder / Q000294:innervation* / Q000473:pathology*; D050494:Vesicular Acetylcholine Transport Proteins / Q000378:metabolism
D000975:Antioxidants; D016900:Neurofilament Proteins; D018696:Neuroprotective Agents; D050494:Vesicular Acetylcholine Transport Proteins; D014451:Ubiquinone; C024989:coenzyme Q10