Transcriptional activation of Cidec by PPARgamma2 in adipocyte.
Cidec is a lipid droplet-associated protein, which inhibits lipolysis, leading to the accumulation of triglycerides in adipocytes. However, the transcriptional regulation of Cidec in adipocyte remains unknown. In the present study we investigated that the mouse Cidec transcript is regulated by PPARgamma2. After the differentiation of adipocyte, the expression pattern of Cidec was similar to that of PPARgamma2. In the presence of a PPARgamma agonist, the level of Cidec mRNA was highly increased. In addition, putative PPRE sites were identified in the Cidec promoter. By chromatin immunoprecipitation assay and reporter assay, we observed the binding of PPARgamma2 to the promoter of Cidec. Gel shift assay and the mutagenesis study were showed that the -219/-207 region of the Cidec promoter could function as a PPRE of the Cidec promoter. These results suggest that PPARgamma2 is required for the transcriptional activity of Cidec during adipogenesis, which could be contributed to understand the molecular mechanism of lipid droplet formation in adipocytes.
Kim
Yoon-Jin
YJ
Department of Biology, Kyung Hee University, Seoul, Republic of Korea.
Cho
Si Young
SY
Yun
Cheol Hee
CH
Moon
Yang Soo
YS
Lee
Tae Ryong
TR
Kim
Sang Hoon
SH
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D015533:Transcriptional Activation
D047495:PPAR gamma; D011506:Proteins; C525805:fat-specific protein 27, mouse