[Study on the genetic characteristics of the avian influenza virus strain A/Zhejiang/16/2006].
OBJECTIVE
To study the genetic characteristics of avian influenza virus strain A/Zhejiang/ 16/2006 which was isolated from the case first reported in Zhejiang province.


METHODS
Complete genome of A/Zhejiang/16/2006 including eight segments were sequenced and compared on the genetic homogeneity with sequences of the similar strains provided through domestic and overseas sources.


RESULTS
There were 11 amino acids showing differences on HA between A/Zhejiang/16/2006 and the H5N1 isolates of neighboring countries, but these differences had not affected the stability of glycosylation sites. In the NA region, 20 amino acids located in the 49th to 68th position were found absent in the isolates obtained after 1997. Eight segments of H5N1 isolates, circulating in the mainland of China in the recent years, formed a separate branch compared to the strains in neighboring countries and there was also obviously different from the strains isolated in Hong Kong and Guangzhou in 1996 and 1997. However, several Chinese strains were close to the Hong Kong strains isolated in 1997 but diffferent from the current strains in the phylogenetic tree.


CONCLUSIONS
The influenza virus strain A/Zhejiang/16/2006 formed a separate branch with the strains isolated in the mainland of China in the past years but it presented an obvious difference with the isolates from the neighboring countries.
Lu
Yi-Yu
YY
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310009, China.
Yan
Ju-Ying
JY
Xu
Chang-Ping
CP
Mao
Hai-Yan
HY
Feng
Yan
Y
Chen
Yin
Y
Li
Zhen
Z
Li
Min-Hong
MH
Shi
Wen
W
D002681:China; D016679:Genome, Viral / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction