Structural and immunochemical analysis of the lipopolysaccharide from Acinetobacter lwoffii F78 located outside Chlamydiaceae with a Chlamydia-specific lipopolysaccharide epitope.
Chemical analyses, NMR spectroscopy, and mass spectrometry were used to elucidate the structure of the rough lipopolysaccharide (LPS) isolated from Acinetobacter lwoffii F78. As a prominent feature, the core region of this LPS contained the disaccharide alpha-Kdo-(2-->8)-alpha-Kdo (Kdo=3-deoxy-d-D-manno-oct-2-ulopyranosonic acid), which so far has been identified only in chlamydial LPS. In serological investigations, the anti-chlamydial LPS monoclonal antibody S25-2, which is specific for the epitope alpha-Kdo-(2-->8)-alpha-Kdo, reacted with A. lwoffii F78 LPS. Thus, an LPS was identified outside Chlamydiaceae that contains a Chlamydia-specific LPS epitope in its core region.
Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 1-40, 23845 Borstel, Germany.
Anna
A
Hanuszkiewicz
Göran
G
Hübner
Evgeny
E
Vinogradov
Buko
B
Lindner
Lore
L
Brade
Helmut
H
Brade
Jennifer
J
Debarry
Holger
H
Heine
Otto
O
Holst
D016954:Acinetobacter calcoaceticus / Q000737:chemistry*; D002689:Chlamydia / Q000276:immunology*; D002693:Chlamydiaceae / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D000939:Epitopes / Q000276:immunology*; D007120:Immunochemistry; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D053719:Tandem Mass Spectrometry
D000939:Epitopes; D008070:Lipopolysaccharides; D009844:Oligosaccharides