Enzyme-linked immunosorbent assay to detect urinary antibody against recombinant rKRP42 antigen made from Leishmania donovani for the diagnosis of visceral leishmaniasis.
We recently reported the production of the recombinant kinesin-related protein of Leishmania donovani with a molecular weight of 42 kd (rKRP42) and the value of the antigen in serum-based ELISA for the diagnosis of visceral leishmaniasis (VL). In this study, the rKRP42 antigen was validated with ELISA using urine samples (rKRP42 urine ELISA). The urine-based ELISA showed 94% sensitivity (108 positives among 115 VL samples) and 99.6% specificity (239 negatives among 240 non-VL samples). The sensitivity and specificity are almost similar to our previous results by ELISA with acetone-treated L. donovani promastigote antigen and direct agglutination test, both methods being done by use of urine samples. A comparison of the rKRP42 urine ELISA with the commercially available urinary antigen detection kit (KAtex) using 108 VL samples showed much higher sensitivity of the ELISA (96.3%) than KAtex (55.6%). The use of the rKRP42 antigen with urine samples will facilitate epidemiologic studies.
Islam
Mohammad Zahidul
MZ
Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan. islammz@yahoo.com
Itoh
Makoto
M
Takagi
Hidekazu
H
Islam
Anwar Ul
AU
Ekram
A R M Saifuddin
AR
Rahman
Ajijur
A
Takesue
Atsuhide
A
Hashiguchi
Yoshihisa
Y
Kimura
Eisaku
E
D000818:Animals; D000913:Antibodies, Protozoan / Q000652:urine*; D000953:Antigens, Protozoan / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000652:urine; D007893:Leishmania donovani / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis*; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D013048:Specimen Handling
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins