Pepper EST database: comprehensive in silico tool for analyzing the chili pepper (Capsicum annuum) transcriptome.
BACKGROUND
There is no dedicated database available for Expressed Sequence Tags (EST) of the chili pepper (Capsicum annuum), although the interest in a chili pepper EST database is increasing internationally due to the nutritional, economic, and pharmaceutical value of the plant. Recent advances in high-throughput sequencing of the ESTs of chili pepper cv. Bukang have produced hundreds of thousands of complementary DNA (cDNA) sequences. Therefore, a chili pepper EST database was designed and constructed to enable comprehensive analysis of chili pepper gene expression in response to biotic and abiotic stresses.


RESULTS
We built the Pepper EST database to mine the complexity of chili pepper ESTs. The database was built on 122,582 sequenced ESTs and 116,412 refined ESTs from 21 pepper EST libraries. The ESTs were clustered and assembled into virtual consensus cDNAs and the cDNAs were assigned to metabolic pathway, Gene Ontology (GO), and MIPS Functional Catalogue (FunCat). The Pepper EST database is designed to provide a workbench for (i) identifying unigenes in pepper plants, (ii) analyzing expression patterns in different developmental tissues and under conditions of stress, and (iii) comparing the ESTs with those of other members of the Solanaceae family. The Pepper EST database is freely available at http://genepool.kribb.re.kr/pepper/.


CONCLUSIONS
The Pepper EST database is expected to provide a high-quality resource, which will contribute to gaining a systemic understanding of plant diseases and facilitate genetics-based population studies. The database is also expected to contribute to analysis of gene synteny as part of the chili pepper sequencing project by mapping ESTs to the genome.
Kim
Hyun-Jin
HJ
Omics Integration Research Center, KRIBB, Yuseong-gu, Daejeon, Korea. hjkim@kribb.re.kr
Baek
Kwang-Hyun
KH
Lee
Seung-Won
SW
Kim
JungEun
J
Lee
Bong-Woo
BW
Cho
Hye-Sun
HS
Kim
Woo Taek
WT
Choi
Doil
D
Hur
Cheol-Goo
CG
D002212:Capsicum / Q000235:genetics*; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D003628:Database Management Systems; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018745:Genome, Plant; D018749:RNA, Plant / Q000235:genetics; D014584:User-Computer Interface
D018076:DNA, Complementary; D018749:RNA, Plant