Evaluation of the hydroxynitrile lyase activity in cell cultures of capulin (Prunus serotina).
Enzymatic preparations obtained from young plants and cell cultures of capulin were screened for hydroxynitrile lyase activity. The three week old plants, grown under sterile conditions, were used to establish a solid cell culture. Crude preparations obtained from this plant material were evaluated for the transformation of benzaldehyde to the corresponding cyanohydrin (mandelonitrile). The results show that the crude material from roots, stalks, and leaves of young plants and calli of roots, stalks, internodes and petioles biocatalyzed the addition of hydrogen cyanide (HCN) to benzaldehyde with a modest to excellent enantioselectivity.
Universidad Autónoma Metropolitana, Unidad Xochimilco, Depto. Sistemas Biológicos, A. P. 23/181, México, D. F., México. hevlilia@servidor.unam.mx
Liliana
L
Hernández
Héctor
H
Luna
Arturo
A
Navarro-Ocaña
Ma Teresa de Jesús
MT
Olivera-Flores
Ivon
I
Ayala
D000097:Acetonitriles / Q000378:metabolism; D000446:Aldehyde-Lyases / Q000378:metabolism*; D001547:Benzaldehydes / Q000378:metabolism; D055162:Biocatalysis; D002478:Cells, Cultured; D006856:Hydrogen Cyanide / Q000378:metabolism; D009570:Nitriles / Q000378:metabolism; D027861:Prunus / Q000166:cytology / Q000201:enzymology*
D000097:Acetonitriles; D001547:Benzaldehydes; D009570:Nitriles; C430593:cyanohydrin; D006856:Hydrogen Cyanide; C045516:mandelonitrile; D000446:Aldehyde-Lyases; C079359:hydroxymandelonitrile lyase; C032175:benzaldehyde