Near full-length genome characterization of an HIV type 1 CRF25_cpx strain from Cameroon.
Recombinant forms of HIV-1 contribute significantly to the ongoing epidemic. In the present study, we characterized the near full-length genome of one candidate HIV-1 CRF25_cpx strain originating in Cameroon, 06CM-BA-040. Viral RNA was extracted from plasma, and the genome was obtained using RT-PCR amplification to generate 10 overlapping fragments. Bootscanning, recombination breakpoint analysis, and phylogenetic trees confirmed that 06CM-BA-040 had a genomic structure consistent with two available CRF25_cpx reference sequences. The CRF25_cpx mosaic composition consisted of nine segments derived from subtypes A and G as well as unclassified (U) regions. Subtype G and CRF25_cpx clusters diverged from each other with long branch lengths but were distinct from other known subtypes with high bootstrap support (94%). The epidemiological significance of CRF25_cpx strains is unknown; however, the availability of additional genomic sequences will improve our understanding of the overall genetic diversity within this recombinant form of HIV-1.
Luk
Ka-Cheung
KC
Abbott Diagnostics, AIDS Research and Retrovirus Discovery, Abbott Park, Illinois 60064, USA.
Holzmayer
Vera
V
Ndembi
Nicaise
N
Swanson
Priscilla
P
Brennan
Catherine A
CA
Ngansop
Charlotte
C
Mbanya
Dora
D
Kaptué
Lazare
L
Gürtler
Lutz
L
Devare
Sushil G
SG
Hackett
John
J
D002163:Cameroon; D016000:Cluster Analysis; D016679:Genome, Viral; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D012367:RNA, Viral