Molecular typing and characterization of extended-spectrum TEM, SHV and CTX-M beta-lactamases in clinical isolates of Enterobacter cloacae.
Sixty-one non-repetitive Enterobacter cloacae ESBL producers were collected at the Amiens University Hospital in France. Eight beta-lactam resistance phenotypes (a-h) and three aminoglycoside resistance phenotypes (i-k) were identified among these isolates, and 32 different pulsotypes were observed. Of these 61 isolates, 37 were sequenced and found to harbor beta-lactamases with a pI of 5.9 (TEM-4), 6.5 (TEM-24), 7.8 (SHV-4), 8.2 (SHV-12), 8.4 (CTX-M-1) and 8.0 (CTX-M-9). Four imipenem-resistant ESBL-producing E. cloacae isolates did not express the 38kDa OMP, indicating that this resistance is associated with porin deficiency.
Biendo
Maurice
M
Service de Bactériologie, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, France. Biendo.Maurice@chu-amiens.fr
Manoliu
Claudia
C
Laurans
Geneviève
G
Castelain
Sandrine
S
Canarelli
Brigitte
B
Thomas
Danièle
D
Hamdad
Farida
F
Rousseau
Florence
F
Eb
François
F
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D016972:Enterobacter cloacae / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D018272:Porins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D055815:Young Adult; D001618:beta-Lactamases / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D018272:Porins; D015378:Imipenem; D001618:beta-Lactamases