[Influence of mutations at genes of global transcriptional regulators on production of autoinducer AI-2 Quorum Sensing in the system of Escherichia coli].
The control of gene expression in response to an increase in the bacterial population density (Quorum Sensing) involves low-molecular-weight signal molecules (autoinducers, AI). AI-2 and synthase LuxS mediating its synthesis are widely distributed in Gram-negative and Gram-positive bacteria. In this work, the data were obtained on the role of global regulators of gene expression in AI-2 synthesis in Escherichia coli cells. The mutation inactivating gene rpoS (encodes sigma S subunit of RNA polymerase) was shown to drastically decrease an amount of active AI-2 in the culture medium. Mutations at gene rpoN that encodes sigma N subunit of RNA polymerase and also at gene lon, which encodes Lon proteinase, on the contrary, increase an amount of active AI-2 in supernatants of cultures. Mutant strains lacking histone-like proteins H-NS and StpA accumulate a slightly higher amount of AI-2 than the isogenic wild-type strain: however, an amount of AI-2 decreased in the culture medium of the double mutant devoid of both these proteins.
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Zavil'gel'skiĭ
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D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D048168:Escherichia coli K12 / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006714:Homoserine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007783:Lactones; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D049070:Protease La / Q000235:genetics / Q000378:metabolism; D053038:Quorum Sensing / Q000502:physiology*; D051877:RNA Polymerase Sigma 54 / Q000235:genetics / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C031908:H-NS protein, bacteria; D007783:Lactones; D018832:Molecular Chaperones; C091088:N-octanoylhomoserine lactone; D012808:Sigma Factor; C090104:StpA protein, E coli; C497751:rpoN protein, E coli; C069567:sigma factor KatF protein, Bacteria; D006714:Homoserine; D051877:RNA Polymerase Sigma 54; C483567:Lon protein, E coli; D049070:Protease La; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria