Electrophysiological properties of NtTPK1 expressed in yeast tonoplast.
The patch clamp technique using enlarged yeast tonoplasts is an effective approach to characterizing the Nicotiana tabacum plant tonoplast K+ channel, NtTPK1. We report here that the NtTPK1-mediated currents comprise two phase components, both which were found to be highly selective for K+ over Na+ and Cl-.
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan.
Shin
S
Hamamoto
Isamu
I
Yabe
Nobuyuki
N
Uozumi
D004594:Electrophysiology; D015870:Gene Expression; D007425:Intracellular Membranes / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D015221:Potassium Channels