Development of a simple and low cost microbioreactor for high-throughput bioprocessing.
A simple microbioreactor for high-throughput bioprocessing made from low cost polymer polytetrafluoroethylene (PTFE) tubes with a working volume of 1.5 ml is described. We have developed a microfluidic system that handles a small population of cells of a model microorganism, Pseudomonas aeruginosa DS10-129. Under the conditions of the microbioreactor, the organism produced extracellular secondary metabolites by using nutrient broth modified with glycerol. Pyocyanins were isolated from the fermented medium as a metabolite of interest. Antibiotic properties of pyocyanin were effective against a number of microorganisms such as Staphylococcus aureus, S. epidermis, Bacillus subtilis, Micrococcus luteus and Saccharomyces cerevisiae. Batch fermentation of the model organism in the microbioreactor was compared to shake-flask and conventional bench fermenter methods. Results obtained from the microbioreactor compared favourably with the conventional processes.
Rahman
Pattanathu K S M
PK
School of Science and Technology, University of Teesside, Middlesbrough, Cleveland, TS1 3BA, UK. p.rahman@tees.ac.uk
Pasirayi
Godfrey
G
Auger
Vincent
V
Ali
Zulfiqur
Z
D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D001681:Biological Assay / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D019149:Bioreactors / Q000191:economics / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008904:Miniaturization; D011710:Pyocyanine / Q000008:administration & dosage*; D006113:United Kingdom
D000900:Anti-Bacterial Agents; D011710:Pyocyanine