Hierarchical assembly of viral nanotemplates with encoded microparticles via nucleic acid hybridization.
We demonstrate hierarchical assembly of tobacco mosaic virus (TMV)-based nanotemplates with hydrogel-based encoded microparticles via nucleic acid hybridization. TMV nanotemplates possess a highly defined structure and a genetically engineered high density thiol functionality. The encoded microparticles are produced in a high throughput microfluidic device via stop-flow lithography (SFL) and consist of spatially discrete regions containing encoded identity information, an internal control, and capture DNAs. For the hybridization-based assembly, partially disassembled TMVs were programmed with linker DNAs that contain sequences complementary to both the virus 5' end and a selected capture DNA. Fluorescence microscopy, atomic force microscopy (AFM), and confocal microscopy results clearly indicate facile assembly of TMV nanotemplates onto microparticles with high spatial and sequence selectivity. We anticipate that our hybridization-based assembly strategy could be employed to create multifunctional viral-synthetic hybrid materials in a rapid and high-throughput manner. Additionally, we believe that these viral-synthetic hybrid microparticles may find broad applications in high capacity, multiplexed target sensing.
Tan
Wui Siew
WS
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Lewis
Christina L
CL
Horelik
Nicholas E
NE
Pregibon
Daniel C
DC
Doyle
Patrick S
PS
Yi
Hyunmin
H
D001483:Base Sequence; D008958:Models, Molecular; D049329:Nanostructures; D009693:Nucleic Acid Hybridization; D014027:Tobacco Mosaic Virus / Q000737:chemistry*