Prostate-tumor-inducing gene-1 analysis in human prostate cancer cells and tissue in relation to Mycoplasma infection.
The potential role of PTI-1, in the natural story of prostate adenocarcinoma remains to be fully determined. PTI-1 expression was evaluated in human prostate cancer cell lines and in paraffin-embedded archive tissues. PTI-1 expression was found in Mycoplasma infected but not in non-infected cells. The lack of PTI-1 expression was also confirmed in fixed and paraffin-embedded human cancer prostate biopsies. The overall data indicate that, in prostate tumor cell lines, PTI-1 presence parallels Mycoplasma infection suggesting that PTI-1 might not necessarily play a major role in the onset of prostate tumorigenesis.
Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Trieste, Italy. bscaggiante@units.it
Bruna
B
Scaggiante
Serena
S
Bonin
Luigi
L
Cristiano
Salvatore
S
Siracusano
Giorgio
G
Stanta
Barbara
B
Dapas
Carlo
C
Giansante
Nicola
N
Fiotti
Gabriele
G
Grassi
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D015153:Blotting, Western; D045744:Cell Line, Tumor / Q000378:metabolism / Q000382:microbiology; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D005189:False Positive Reactions; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology; D015972:Gene Expression Regulation, Neoplastic; D022781:Hepatocytes / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D008297:Male; D045790:Mycoplasma hyorhinis / Q000235:genetics*; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D020648:Peptide Elongation Factor 1 / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
C094814:EEF1A1 protein, human; D009363:Neoplasm Proteins; D020648:Peptide Elongation Factor 1; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012336:RNA, Ribosomal, 16S