[Sensitivity and specificity of the VIKIA Rota-Adeno immuno-chromatographic test (bioMérieux) and the ELISA IDEIA Rotavirus kit (Dako) compared to genotyping].
The performances of two diagnostic tests for rotavirus infection in stool samples were evaluated during a prospective study in children of less than 36 months in child-care centers of Lyon from November 2004 to May 2005. The VIKIA Rota-Adeno immuno-chromatographic test (bioMérieux) and the ELISA IDEIA Rotavirus kit (Dako) were compared with a referral method, the genotyping. Fifty-seven stool samples were collected and analyzed by RT-PCR. The virus genome was detected in 29 samples. The most frequent genotypic combinations were G9P[8] with a prevalence of 75.9%. Sensitivity and specificity of the VIKIA Rota-Adeno test and the ELISA IDEIA Rotavirus kit were strictly comparable and very good: 96.6% (83.0; 99.9) and 96.4% (81.6; 99.9), respectively. The immuno-chromatographic technique were in concordance with the ELISA tests in 93.6% of cases. Thus, the VIKIA Rota-Adeno test is a good alternative for the occasional analysis of stool samples in ambulatory practice.
de Rougemont
A
A
Centre national de référence des virus entériques, laboratoire de virologie, plateau technique de biologie, CHU de Dijon, 2, rue Angélique-Ducoudray, BP 37013, 21070 Dijon cedex, France.
Kaplon
J
J
Billaud
G
G
Lina
B
B
Pinchinat
S
S
Derrough
T
T
Caulin
E
E
Pothier
P
P
Floret
D
D
D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D002656:Child Day Care Centers; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods; D003124:Colorimetry; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000821:virology; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005260:Female; D005602:France / Q000453:epidemiology; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007223:Infant; D008297:Male; D017921:Mastadenovirus / Q000235:genetics / Q000276:immunology; D011446:Prospective Studies; D012401:Rotavirus / Q000235:genetics / Q000276:immunology; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral
Sensibilité et spécificité du test immuno-chromatographique VIKIA Rota-Adéno (bioMérieux) et du kit Elisa IDEIA Rotavirus (Dako) par rapport aux techniques de génotypage.