Chemokine receptor repertoire reflects mature T-cell lymphoproliferative disorder clinical presentation.
The World Health Organization classification of mature T-cell lymphoproliferative disorders, combines clinical, morphological and immunophenotypic data. The latter is a major contributor to the classification, as well as to the understanding of the malignant T-cell behavior. The fact that T-cell migration is regulated by chemokines should, in theory, enable us to identify tissue tropism and organ involvement by neoplastic T-cells by monitoring chemokine receptor surface expression. To address this issue we compared the expression of several early and late inflammatory, homeostatic, and organ specific chemokine receptors on blood T-cells from normal individuals and patients with T-cell large granular lymphocytic leukemia and peripheral T-cell lymphoma. T-cell large granular lymphocytic leukemia cells mainly express late inflammatory chemokine receptors (CXCR1 and CXCR2), whereas peripheral T-cell lymphoma cells usually express one or more organ homing receptors (CCR4, CCR6 and CCR7). Nevertheless, no clear correlation was found between CCR4 and CCR7 expression and skin and lymph node involvement, respectively. Compared to their normal counterparts, lymphoma T-cells displayed an exaggerated CCR4 expression, whereas leukemic T-cells had abnormally high CXCR1 and CXCR2 expression. Further analysis revealed that, in leukemia patients, the percentage of neoplastic cells expressing CCR5 correlates directly with lymphocytosis. In addition, in the case of CD8 T-cell leukemia patients, an inverse correlation with neutropenia was found. In lymphoma patients, higher CCR4 and CCR7 expression is accompanied by lower to absent CCR5 expression.
Laboratório de Citometria, Serviço de Hematologia Clínica, Centro Hospitalar do Porto (CHP), Hospital Geral de Santo António (HGSA), Porto, Portugal. jmouraalves@gmail.com
João
J
Moura
João
J
Rodrigues
Ana Helena
AH
Santos
Maria dos Anjos
Mdos A
Teixeira
Maria Luís
ML
Queirós
Marlene
M
Santos
Marta
M
Gonçalves
Sónia
S
Fonseca
Carla
C
Laranjeira
António Silva
AS
Rodrigues
Esmeraldina
E
Correia Júnior
Fernanda
F
Ribeiro
Maria João
MJ
Acosta
Margarida
M
Lima
D016207:Cytokines / Q000276:immunology; D006801:Humans; D015458:Leukemia, T-Cell / Q000145:classification* / Q000175:diagnosis* / Q000276:immunology; D016399:Lymphoma, T-Cell / Q000145:classification* / Q000175:diagnosis* / Q000276:immunology; D019707:Receptors, Chemokine / Q000032:analysis / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology
D016207:Cytokines; D019707:Receptors, Chemokine