Comparison of Sebia Capillarys capillary electrophoresis with the Primus high-pressure liquid chromatography in the evaluation of hemoglobinopathies.
The Sebia Capillarys (capillary zone electrophoresis [CE]) and the Primus Resolution high-pressure liquid chromatography (HPLC) were used to prospectively evaluate 297 samples for hemoglobinopathies. Hemoglobin (Hb) A levels were similar on both techniques (mean, 96.2% and SD, 5.7% by CE; mean, 96.8% and SD, 5.5% by HPLC), but HbA2 levels were higher by CE (mean, 2.8%; SD, 0.8%) than by HPLC (mean, 2.3%; SD, 0.8%). HbS had higher values by CE (mean, 40.6%; SD, 18.9%) than by HPLC (mean, 38.4%; SD, 18.9%). In cases with Hg S, HbA2 levels were greater by HPLC (mean, 4.0%; SD, 1.0%) than by CE (mean, 3.1%; SD, 0.8%). HbA2 was occasionally not separated sufficiently from HbC for measurement by CE, but did separate from HbE by CE. Both methods identified HbS, HbC, HbE, HbS, and HbC together, HbA2&prime, HbD-Los Angeles, HbF variant, HbG-Philadelphia, HbS-G Philadelphia, and Hb Lepore.
Keren
David F
DF
Warde Medical Laboratory, St Joseph Mercy Hospital, Ann Arbor, MI 48108, USA.
Hedstrom
Deborah
D
Gulbranson
Ronald
R
Ou
Ching-Nan
CN
Bak
Richard
R
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D019075:Electrophoresis, Capillary / Q000295:instrumentation; D006441:Hemoglobin A / Q000032:analysis; D006443:Hemoglobin A2 / Q000032:analysis; D006444:Hemoglobin C / Q000032:analysis; D006446:Hemoglobin E / Q000032:analysis; D006451:Hemoglobin, Sickle / Q000032:analysis; D006453:Hemoglobinopathies / Q000175:diagnosis*; D006455:Hemoglobins, Abnormal / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D011446:Prospective Studies; D015203:Reproducibility of Results
D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; D006444:Hemoglobin C; D006441:Hemoglobin A; D006443:Hemoglobin A2; D006446:Hemoglobin E