Systemic resistance in Arabidopsis conferred by the mycorrhizal fungus Piriformospora indica requires jasmonic acid signaling and the cytoplasmic function of NPR1.
We analyzed the requirement of specific defense pathways for powdery mildew (Golovinomyces orontii) resistance induced by the basidiomycete Piriformospora indica in Arabidopsis. Piriformospora indica root colonization reduced G. orontii conidia in wild-type (Col-0), npr1-3 (nonexpressor of PR genes 1-3) and NahG plants, but not in the npr1-1 null mutant. Therefore, cytoplasmic but not nuclear localization of NPR1 is required for P. indica-induced resistance. Two jasmonate signaling mutants were non-responsive to P. indica, and jasmonic acid-responsive vegetative storage protein expression was primed and thus elevated in response to powdery mildew, suggesting that P. indica confers resistance reminiscent of induced systemic resistance (ISR).
Institute of Phytopathology and Applied Zoology, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
Elke
E
Stein
Alexandra
A
Molitor
Karl-Heinz
KH
Kogel
Frank
F
Waller
D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001203:Ascomycota / Q000472:pathogenicity*; D001487:Basidiomycota / Q000502:physiology*; D003517:Cyclopentanes / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D007113:Immunity, Innate; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D018744:DNA, Plant; C105496:NPR1 protein, Arabidopsis; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; D020156:Salicylic Acid