Thymodepressin inhibits migration of CD34+ cells from bone marrow in normal and granulocyte CSF-stimulated hemopoiesis.
We studied the effect of thymodepressin on migration and adhesion of mouse hemopoietic CD34+ cells under normal conditions and under the effect of granulocytic CSF. It was found that the peptide reduced the absolute number of CD34+ hemopoietic cells in the peripheral blood, increased the percent of cells bound to fibronectin and expressing receptor for integrin beta1 (CD29+) in the bone marrow of mice under normal conditions and after stimulation with granulocytic CSF, and reduced the relative number of cells carrying CXCR4 receptor for stromal factor-1 (CD184+) in the bone marrow (CD34+CD184+) and blood (CD184+) of mice stimulated with granulocytic CSF. The results suggest that thymodepressin can inhibit migration of CD34+ cells from bone marrow into peripheral blood under conditions of normal and granulocytic CSF-stimulated hemopoiesis.
Semina
O V
OV
Medical Radiological Research Center, Russian Academy of Medical Sciences, Obninsk. ovs45@mail.ru
Semenets
T N
TN
Zamulaeva
I A
IA
Selivanova
E I
EI
Il'ina
T P
TP
Malyutina
Ya V
YV
Semin
D Yu
DY
Deigin
V I
VI
Saenko
A S
AS
D000818:Animals; D018952:Antigens, CD34 / Q000187:drug effects / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000494:pharmacology*
D018952:Antigens, CD34; D010455:Peptides; D016179:Granulocyte Colony-Stimulating Factor; C449663:thymodepressin