[Effects of toll-like-receptor-9 expression in chronic rhinosinusitis with nasal polyps].
OBJECTIVE
To determine the presence of TLR-9 protein in primary epithelial cell cultures of chronic rhinosinusitis with nasal polyps (CRSwNP), and then explore the role of innate immune recognition in the pathogenesis of CRSwNP.


METHODS
Primary epithelial cell cultures were established in 10 controls and 10 CRSwNP patients who underwent sinus surgery, and flow cytometry was used to confirm its purity and measure the expression of TLR-9 protein.


RESULTS
By digestive method 98% of cultured primary nasal mucosa cells were epithelial cells and the expression of TLR-9 protein in CRSwNP group (11%-15%) was lower than that in normal group (49%-60%).


CONCLUSIONS
This finding suggests that impaired innate immune responses via TLR-9 on sinonasal epithelial cells may involve in pathogenesis of CRSwNP.
Department of Otolaryngology, Union Hospital Affiliated of Huazhong University of Science and Technology, Wuhan, 430022, China.
Zhongfang
Z
Xia
Weijia
W
Kong
Jianxin
J
Yue
Yanjun
Y
Wang
Lisha
L
Wu
D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D002908:Chronic Disease; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D009298:Nasal Polyps / Q000276:immunology / Q000378:metabolism*; D012852:Sinusitis / Q000276:immunology / Q000378:metabolism*; D051217:Toll-Like Receptor 9 / Q000378:metabolism*; D055815:Young Adult
C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9