[Study of applying fluorescence spectrum imaging to quantitative assay of proteins in bio-chip].
In the present work, the amount and the activity of the goat anti-human IgG, to bind the human IgG labelled with fluorescein isothiocyanate (FITC), immobilized on silicon surfaces modified with APTES and APTES-Glu, respectively, were studied using the fluorescence spectrum imaging (FSI), the results of which were compared with that of ellipsometry. It is shown that the amount of the human IgG labeled with FITC on APTES-Glu measured using FSI is 2.8 times higher than that on APTES, which is nearly coincident with the 2.2 times obtained using ellipsometry, showing that the activity of the goat anti-human IgG on APTES-Glu is higher than that on APTES. It is reasoned that the FSI is used in the fluorescence immunoassay the for measurement of quasi-quantification or quantification.
Key Laboratory of Excited State Processes, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China.
Ke-Fu
KF
Chao
You-Lin
YL
Zhang
Xiang-Gui
XG
Kong
Bing
B
Li
Qing-Hui
QH
Zeng
Kai
K
Song
Ya-Juan
YJ
Sun
D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D007118:Immunoassay; D040081:Protein Array Analysis; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D011506:Proteins; D016650:Fluorescein-5-isothiocyanate