Production and partial purification of alpha-amylase from a novel isolate Streptomyces gulbargensis.
Extracellular amylase production by a newly isolated alkali-thermotolerant strain Streptomyces gulbargensis DAS 131 was optimized and characterized. The highest amylase production was achieved by growing S. gulbargensis DAS 131 in media with 1% starch. Strain exhibited maximal activity at pH 9.0 and 45 degrees C and relatively stable in alkaline conditions (pH 11). Starch and peptone were found to be the good source of carbon and nitrogen with a yield of 2,216.6 and 2,156.1 U, respectively. Maltose and maltotriose were the main end products of starch hydrolysis, indicating alpha-amylase activity. SDS-PAGE analysis revealed a monomeric form with a molecular weight of 55 kDa.
Syed
Dastager G
DG
Biotechnology Division, National Institute of Interdisciplinary Science and Technology (CSIR), Industrial Estate, Pappanamcode, Thiruvananthapuram, 695019, India. syed_micro@rediffmail.com
Agasar
Dayanand
D
Pandey
Ashok
A
D001709:Biotechnology; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007194:India; D010461:Peptones / Q000378:metabolism; D012988:Soil Microbiology; D013213:Starch / Q000378:metabolism; D013302:Streptomyces / Q000145:classification / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification*; D013696:Temperature; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000302:isolation & purification*
D003470:Culture Media; D010461:Peptones; D013213:Starch; D000516:alpha-Amylases