Effects of cisplatin and other Pt(II) complexes on spontaneous motility of isolated human oviduct.
The toxicity of platinum(II) ion could be significantly modified by coordination with some organic compounds. In our study, the cytotoxicity and the influence of platinum(II) complexes, such as cisplatin, cis-[PtCl(2)(NH(3))(2)], [PtCl(2)(SMC)] and [PtCl(2)(DMSO)(2)] (where SMC is S-methyl-l-cysteine and DMSO is dimethyl sulphoxide) on spontaneous motility of isolated human fallopian tubes were investigated. Cisplatin showed potent pro-apoptotic effects on peripheral blood mononuclear cells (PBMC). However, peripheral blood mononuclear cells were substantially less sensitive to [PtCl(2)(SMC)] and [PtCl(2)(DMSO)], and these compounds showed no toxic effect on PBMC in all concentrations examined. Cisplatin showed concentration-dependent inhibition of spontaneous contractions of the isolated ampulla. (EC(50)=1.14+/-0.03 x 10(-6)M/l, r=0.714, p<0.05) while [PtCl(2)(SMC)] and [PtCl(2)(DMSO)(2)] did not affect spontaneous contractions of isolated fallopian tube ampulla.
University of Kragujevac, Faculty of Sciences, Department of Chemistry, Radoja Domanovića 12, P.O. Box 60, 34000 Kragujevac, Serbia. bugarcic@kg.ac.yu
Zivadin D
ZD
Bugarcić
Biljana
B
Petrović
Zorica M
ZM
Bugarcić
Slobodan M
SM
Janković
Snezana V
SV
Janković
Goran
G
Lukić
Milan
M
Novaković
Vladimir
V
Kostović
Suzana
S
Popović
Predrag
P
Djurdjević
Dejan
D
Baskić
Nebojsa
N
Arsenijević
D000328:Adult; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D003545:Cysteine / Q000031:analogs & derivatives / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005187:Fallopian Tubes / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism
D000970:Antineoplastic Agents; C008425:S-methylcysteine; D003545:Cysteine; D002945:Cisplatin; D004121:Dimethyl Sulfoxide