Phylogenetic analysis and siderotyping as useful tools in the taxonomy of Pseudomonas stutzeri: description of a novel genomovar.
An examination of the results of phylogenetic analyses based on the sequences of fragments of the 16S rRNA, gyrB and rpoD genes, and the discrimination of genomovars based on siderophore diversity within the genus Pseudomonas, has added important taxonomic tools in the characterization of Pseudomonas stutzeri. Eighteen reference strains, nine newly identified hydrocarbon-degrading strains and three strains showing relevant physiological characteristics of P. stutzeri, together with the type strains of four related species, were included in the study. A novel genomovar within the species is described. A summary of the methodology used in these studies and the results of our attempts to define a solid internal subdivision of this important species within the genus Pseudomonas are presented and discussed.
Mulet
Magdalena
M
Departament de Biologia, Microbiologia and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain.
Gomila
Margarita
M
Gruffaz
Christelle
C
Meyer
Jean-Marie
JM
Palleroni
Norberto J
NJ
Lalucat
Jorge
J
García-Valdés
Elena
E
D015373:Bacterial Typing Techniques; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005798:Genes, Bacterial; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D044223:Pseudomonas stutzeri / Q000145:classification* / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000235:genetics*; D012808:Sigma Factor / Q000235:genetics
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D027081:DNA Gyrase