Effect of crude sulphated polysaccharide from brown algae against acetaminophen-induced toxicity in rats.
This study was conducted to examine the protective role of crude polysaccharide from brown seaweed Sargassum polycystum against acetaminophen-induced abnormality in blood glucose, serum albumin/globulin ratio, and liver glycogen, lactate, and pyruvate. Liver and renal tissue histology was performed to confirm the efficacy of Sargassum polysaccharide. A toxic dose of acetaminophen (800 mg/kg body weight intraperitoneally) induced severe abnormality in all basic parameters with apparent toxicity in liver (enlargement of hepatocytes, loss of cytoplasmic content with disruption in the hepatic plates and sinusoidal dilation) and renal tissue (glomerular damage with congestion of tubules). The isolated liver cells were stained with acridine orange and examined under fluorescence microscope, which revealed that the acetaminophen induced significant damage. In contrast, the rats pretreated with Sargassum polysaccharide (200 mg/kg body weight) daily for 3 weeks did not show liver and renal tissue with these severe aberrations induced by acetaminophen. Histology results were also consistent with analyzed basic biochemical parameters, which confirmed the effectiveness of the crude polysaccharide against acetaminophen-induced abnormality in rats.
Radiation Food Science and Biotechnology Team, Advanced Radiation Technology Institute, KAERI, Jeongeup, 580-185, Republic of Korea. hbr_bala@yahoo.com
Hanumantha Rao Balaji
HR
Raghavendran
Periasamy
P
Srinivasan
D000082:Acetaminophen / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D062787:Drug Overdose; D005916:Globulins / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000473:pathology; D008111:Liver Function Tests; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000378:metabolism
D018712:Analgesics, Non-Narcotic; D001786:Blood Glucose; D005916:Globulins; D008112:Liver Glycogen; D011134:Polysaccharides; D012709:Serum Albumin; D019344:Lactic Acid; D000082:Acetaminophen; D019289:Pyruvic Acid