Biophysical studies with parallel stranded oligoduplex.
The thermal denaturation profiles of parallel stranded oligoduplexes during UV-spectroscopy and fluorescence spectroscopy, for the first time, were shown to follow the similar patterns as were described for antiparallel stranded oligoduplexes. The B-form structure of these center-bubble-forming oligoduplexes was determined with circular dichroism. Thermodynamic properties were also discussed.
Laboratory 332, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India. pbhattacharya@mail.jnu.ac.in
Pradip
P
Bhattacharya
Ramesh K
RK
Bamezai
D001483:Base Sequence; D055592:Biophysical Phenomena; D002942:Circular Dichroism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry* / Q000235:genetics; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013816:Thermodynamics
D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides