Selective contribution of Tyk2 to cell activation by lipopolysaccharide.
Tyk2 deficient mice show a markedly reduced susceptibility to lipopolysaccharide (LPS) induced shock and a partial impairment of IL-12 and interferon (IFN) signals. To examine the underlying mechanisms, we analysed the activation of peritoneal macrophages (PMPhis) and spleen cells after LPS challenge. In PMPhis and spleen cells the contribution of Tyk2 to the induction of the T-cell co-stimulatory molecules CD86, CD40 and MHC II was small or insignificant. By contrast, induced expression of the early activation marker CD69 on PMPhis and splenic cell populations required type I interferons (IFN-I) and Tyk2. The data suggest a selective contribution of Tyk2 to the activation of inflammation-relevant cell types by LPS.
Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.
Andrea
A
Schneider
Ursula
U
Reichart
Wilhelm
W
Gerner
Thomas
T
Kolbe
Armin
A
Saalmüller
Mathias
M
Müller
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology; D007370:Interferon Type I / Q000276:immunology; D007372:Interferons / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D037181:Lectins, C-Type; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation / Q000235:genetics; D008262:Macrophage Activation / Q000235:genetics; D017737:Macrophages, Peritoneal / Q000201:enzymology / Q000276:immunology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D053633:Receptor, Interferon alpha-beta / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology; D053634:TYK2 Kinase / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D051940:B7-2 Antigen; D007370:Interferon Type I; D007372:Interferons; D018664:Interleukin-12; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D053633:Receptor, Interferon alpha-beta; D053634:TYK2 Kinase; D000099192:CD69 Antigens; C499356:Tyk2 protein, mouse