Propionate sensor using coenzyme-A transferase and acyl-CoA oxidase.
We developed an amperometric propionate sensor using comprised of two recombinant enzymes, propionate coenzyme A CoA transferase from Clostridium propionicum and short-chain acyl-CoA oxidase from Arabidopsis thaliana. Response current increased linearly with increase in propionate concentration from 10 microM to 100 microM. The detection limit was 10 microM propionate.
Department of Biotechnology, Tokyo University of Agriculture and Technology, Naka-cho, Koganei, Tokyo 184-8588 Japan. sode@cc.tuat.ac.jp
Koji
K
Sode
Wakako
W
Tsugawa
Minoru
M
Aoyagi
Eranna
E
Rajashekhara
Kazuya
K
Watanabe
D042962:Acyl-CoA Oxidase / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology*; D015374:Biosensing Techniques / Q000379:methods*; D003013:Clostridium / Q000201:enzymology*; D015256:Coenzyme A-Transferases / Q000378:metabolism*; D004563:Electrochemistry; D011422:Propionates / Q000378:metabolism*
D011422:Propionates; D042962:Acyl-CoA Oxidase; D015256:Coenzyme A-Transferases; C031212:acyl-CoA transferase