The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens.
Epitope-based vaccines designed to induce cellular immune response and antibody responses specific for infectious bronchitis virus (IBV) are being developed as a means for increasing vaccine potency. In this study, we selected seven epitopes from the spike (S1), spike (S2), and nucleocapsid (N) protein and constructed a multi-epitope DNA vaccine. The 7-day-old chickens were immunized intramuscularly with multi-epitope DNA vaccine encapsulated by liposome and boosted two weeks later, and were challenged by virulent IBV strain five weeks post booster. The results showed that multi-epitope DNA vaccine led to a dramatic augmentation of humoral and cellular responses, and provided up to 80.0% rate of immune protection. The novel immunogenic chimeric multi-epitope DNA vaccine revealed in this study provided a new candidate target for IBV vaccine development.
Tian
Lang
L
College of Veterinary Medicine, Sichuan Agricultural University, Yaan, People's Republic of China.
Wang
Hong-ning
HN
Lu
Dan
D
Zhang
Yun-fei
YF
Wang
Ting
T
Kang
Run-ming
RM
D000818:Animals; D000917:Antibody Formation; D019556:COS Cells; D002645:Chickens / Q000276:immunology* / Q000821:virology; D002522:Chlorocebus aethiops; D000086462:Coronavirus Nucleocapsid Proteins; D007111:Immunity, Cellular; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology*; D001351:Infectious bronchitis virus / Q000235:genetics / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology; D064370:Spike Glycoprotein, Coronavirus; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000086462:Coronavirus Nucleocapsid Proteins; D016056:Immunodominant Epitopes; D008562:Membrane Glycoproteins; D019590:Nucleocapsid Proteins; D064370:Spike Glycoprotein, Coronavirus; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines