[The analysis of estrogen receptor alpha (ER-alpha) gene Pvull and Xbal polymorphisms in postmenopausal women with breast cancer].
Estrogens play a crucial role in the pathogenesis and progression of breast and endometrial cancer. The gene ER-alpha is polymorphic and gene variability could contribute to the level of protein biosynthesis. In the present work the distribution of genotypes and frequency of alleles of the Pvull polymorphism and Xbal polymorphism of ER-alpha gene in subjects with breast cancer were investigated.


METHODS
Blood samples were obtained from 103 postmenopausal women with breast cancer. The polymorphisms were determined by PCR-RFLP.


RESULTS
The distribution of the genotypes of Pvull and Xbal polymorphism of ER-alpha in both control and patients did not differ significantly (p > 0.05) from those predicted by the Hardy-Weinberg distribution. There were no significant differences (p > 0.05) in genotype distributions and allele frequencies between subgroups assigned to histological stage.


CONCLUSIONS
The results suggest that the Pvull polymorphism of ER-alpha gene as well as Xbal polymorphism may not be linked with appearance and development of breast cancer.
Department of Menopausal Disease, Institute of Polish Mother's Memorial Hospital, Lódź, Poland.
Anna
A
Sobczuk
Tomasz
T
Pertyński
Beata
B
Smolarz
Hanna
H
Romanowicz-Makowska
D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics*; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic; D017698:Postmenopause
C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha
Analiza polimorfizmów Pvull i Xbal genu receptora estrogenowego alpha (ER-alpha) u kobiet chorych na raka piersi w wieku pomenopauzalnym.