Commercial antibodies: the good, bad, and really ugly.
The range of antibodies available commercially grows ever larger. Perhaps as a consequence, quality control is not always what it could and should be. Investigators must be aware of potential pitfalls and take steps to assure themselves that the specificity of each antibody is as advertised. Additionally, companies should provide the necessary information about the antigen and antibody to investigators, including references, so that the appropriate controls can be included.
Couchman
John R
JR
Biomedicine Institute, University of Copenhagen, Biocenter, Ole Maaloes Vej 5, 2200 Copenhagen N, Denmark. john.couchman@bric.dk
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D011786:Quality Control; D011933:Reagent Kits, Diagnostic / Q000592:standards*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic