Oropouche virus entry into HeLa cells involves clathrin and requires endosomal acidification.
Oropouche virus (ORO), family Bunyaviridae, is the second most frequent cause of arboviral febrile illness in Brazil. Studies were conducted to understand ORO entry in HeLa cells. Chlorpromazine inhibited early steps of ORO replication cycle, consistent with entry/uncoating. The data indicate that ORO enters HeLa cells by clathrin-coated vesicles, by a mechanism susceptible to endosomal acidification inhibitors. Transmission electron microscopy and immunofluorescence indicated that ORO associates with clathrin-coated pits and can be found in association with late endosomes in a time shorter than 1h.
Santos
Rodrigo I M
RI
Department of Cell and Molecular Biology, University of São Paulo School of Medicine at Ribeirão Preto, Brazil. risantos@usp.br
Rodrigues
Alcir H
AH
Silva
Maria Lúcia
ML
Mortara
Renato A
RA
Rossi
Marcos A
MA
Jamur
Maria Célia
MC
Oliver
Constance
C
Arruda
Eurico
E
D000143:Acids / Q000378:metabolism*; D001938:Brazil; D002044:Bunyaviridae Infections / Q000378:metabolism* / Q000821:virology; D022163:Clathrin-Coated Vesicles / Q000378:metabolism* / Q000648:ultrastructure / Q000821:virology; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure / Q000821:virology; D006367:HeLa Cells; D006801:Humans; D016851:Orthobunyavirus / Q000502:physiology* / Q000648:ultrastructure; D053586:Virus Internalization
D000143:Acids