In vitro and in vivo studies evaluating antisemen antibodies as a potential spermicidal agent in hamsters.
OBJECTIVE
To determine the spermicidal activity of antisemen antibodies in the hamster model.


METHODS
Prospective, controlled study.


METHODS
Advanced preclinical sciences center.


METHODS
Subgroups of 10 and 14 golden hamsters.


METHODS
Ex vitro and in vivo treatment of sperm with antisemen antibodies or normal rabbit serum.


METHODS
The EC(50) value of antisemen antibodies, the time required for 50% motility loss of progressively motile spermatozoa exposed to antisemen antibodies, the average sperm mitochondrion fluorescence intensity, the rate of fertilization, and the scoring of histologic changes in the hamster vaginal tissue.


RESULTS
The EC(50) value of antisemen antibodies was found 70 microg/mL, and the time required for 50% motility loss of progressively motile spermatozoa exposed to antisemen antibodies (at 70 microg/mL) was 5 minutes; for the experimental and control groups, the average fluorescence intensities of sperm mitochondria were respectively 180.28 +/- 82.24 and 309.74 +/- 148.37, the fertilization rates in vitro were 0.09% and 45%, the rates of fertilization with intrauterine sperm injection were 0 and 15.0%. There was a significant difference between two groups. None of the four hamsters that received antisemen antibodies in gel-polyoxyl-40-stearate had epithelial disruption characteristic of inflammation.


CONCLUSIONS
Antisemen antibodies possess appreciable spermicidal potential, which may be explored as an effective constituent of spermicide.
Research Center for Reproductive Medicine, Shantou University Medical College, Shantou, Guangdong, People's Republic of China 515041.
De-Yu
DY
Chen
Research Center for Reproductive Medicine, Shantou University Medical College, Shantou, Guangdong, People's Republic of China 515041.
Li-Min
LM
Liu
Research Center for Reproductive Medicine, Shantou University Medical College, Shantou, Guangdong, People's Republic of China 515041.
Qing-Dong
QD
Xie
Research Center for Reproductive Medicine, Shantou University Medical College, Shantou, Guangdong, People's Republic of China 515041.
Lan
L
Xu
Research Center for Reproductive Medicine, Shantou University Medical College, Shantou, Guangdong, People's Republic of China 515041. Electronic address: thhuang@stu.edu.cn.
Tian-Hua
TH
Huang
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000494:pharmacology*; D006224:Cricetinae; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005260:Female; D005307:Fertilization in Vitro; D066298:In Vitro Techniques; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008647:Mesocricetus; D023421:Models, Animal; D011817:Rabbits; D012661:Semen / Q000276:immunology*; D013081:Sperm Motility / Q000187:drug effects*; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013089:Spermatocidal Agents / Q000494:pharmacology*; D014621:Vagina / Q000187:drug effects / Q000473:pathology; D015044:Zona Pellucida / Q000187:drug effects / Q000473:pathology
D000888:Antibodies, Anti-Idiotypic; D013089:Spermatocidal Agents