Hydrogen peroxide resistance of Acetobacter pasteurianus NBRC3283 and its relationship to acetic acid fermentation.
The bacterium Acetobacter pasteurianus can ferment acetic acid, a process that proceeds at the risk of oxidative stress. To understand the stress response, we investigated catalase and OxyR in A. pasteurianus NBRC3283. This strain expresses only a KatE homolog as catalase, which is monofunctional and growth dependent. Disruption of the oxyR gene increased KatE activity, but both the katE and oxyR mutant strains showed greater sensitivity to hydrogen peroxide as compared to the parental strain. These mutant strains showed growth similar to the parental strain in the ethanol oxidizing phase, but their growth was delayed when cultured in the presence of acetic acid and of glycerol and during the acetic acid peroxidation phase. The results suggest that A. pasteurianus cells show different oxidative stress responses between the metabolism via the membrane oxidizing pathway and that via the general aerobic pathway during acetic acid fermentation.
Okamoto-Kainuma
Akiko
A
Department of Fermentation Science, Tokyo University of Agriculture, Tokyo, Japan. okamoto@nodai.ac.jp
Ehata
Yasunori
Y
Ikeda
Manami
M
Osono
Takemasa
T
Ishikawa
Morio
M
Kaga
Takayuki
T
Koizumi
Yukimichi
Y
D019342:Acetic Acid / Q000378:metabolism*; D000091:Acetobacter / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D002374:Catalase / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D005285:Fermentation / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D006861:Hydrogen Peroxide; D002374:Catalase; D019342:Acetic Acid