Spectroscopic properties and bacteriochlorophyll c isomer composition of extramembranous light-harvesting complexes in the green sulfur photosynthetic bacterium Chlorobium tepidum and its CT0388-deleted mutant under vitamin B12-limited conditions.
The effects of exogenous vitamin B12 on the green sulfur photosynthetic bacterium Chlorobium (Chl.) tepidum were examined. Wild-type cells and mutant cells lacking a gene CT0388 (denoted as VB0388) of Chl.tepidum were grown in liquid cultures containing different concentrations of vitamin B12. The VB0388 cells hardly grew in vitamin B12-limited media, indicating that the product of CT0388 actually played an important role in vitamin B12 biosynthesis in Chl. tepidum. Both wild-type and VB0388 cells in vitamin B12-limited media exhibited absorption bands and CD signals at the Qy region that were shifted to a shorter wavelength than those of cells grown in normal media. BChl c isomers that had S-stereochemistry at the 3(1)-position tended to increase in Chl. tepidum grown in vitamin B12-limited media.
Saga
Yoshitaka
Y
Department of Chemistry, Faculty of Science and Engineering, Kinki University, Higashi-Osaka, Osaka 577-8502, Japan. saga@chem.kindai.ac.jp
Harada
Jiro
J
Hattori
Hiromitsu
H
Kaihara
Kanako
K
Hirai
Yuki
Y
Oh-oka
Hirozo
H
Tamiaki
Hitoshi
H
D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry*; D001429:Bacteriochlorophylls / Q000032:analysis* / Q000737:chemistry*; D041883:Chlorobium / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004305:Dose-Response Relationship, Drug; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D008968:Molecular Conformation; D009154:Mutation / Q000235:genetics*; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry; D013053:Spectrophotometry; D014805:Vitamin B 12 / Q000494:pharmacology*
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D020033:Protein Isoforms; C002084:bacteriochlorophyll c; D014805:Vitamin B 12