[Construction and analysis of root cDNA library in Glycyrrhiza uralensis].
OBJECTIVE
To screen and isolate secondary metabolite biosynthesis-related gene for establishing the foundation of functional gene research, we construct a cDNA library of Glycyrrhiza uralensis.


METHODS
Total RNA was isolated from G. uralensis using the method of lithium chloride sedimentation. Double strand cDNA was joined into pBlueScript II vector. The number of clones, recombinant rate and length of insert fragments were determined.


RESULTS
The capacity of the original library was 1.15 x 10(7) with a recombinant rate of 98.2% and the inserted cDNA fragments ranged from 0.5 to 4.8 kb. 126 ESTs through random sequencing were obtained. The most homological proteins came from leguminous plants, including Arabidopsis thaliana, Oryza sativa, and so on. Most of the proteins were related to genes linking cell matabolism, resistance, growth retardation and dormancy.


CONCLUSIONS
The library has enough capacity, high recombinant rate and long insert fragment for the further study.
Yang
Quan
Q
School of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zhang
Hui
H
Wang
Wen-quan
WQ
Liu
Chun-sheng
CS
Wei
Sheng-li
SL
D019295:Computational Biology; D004274:DNA, Recombinant / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D020224:Expressed Sequence Tags; D015723:Gene Library; D029882:Glycyrrhiza uralensis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA
D004274:DNA, Recombinant; D012333:RNA, Messenger; D018749:RNA, Plant