Increased spermine oxidase (SMO) activity as a novel differentiation marker of myogenic C2C12 cells.
Spermine oxidase (SMO) is a FAD-containing enzyme involved in animal cell polyamines (PA) homeostasis, selectively active on spermine and producing H(2)O(2), spermidine, and the 3-aminopropanal. In the present study, we have examined the SMO gene expression during the mouse myoblast C2C12 cell differentiation induced with two different stimuli by RT-PCR analysis, polysome-mRNP distribution and enzyme activity. SMO transcript accumulation and enzymatic activity increases during C2C12 cell differentiation and correlates with the decrease of spermine content. Many proteins are highly regulated during the phenotypic conversion of rapidly dividing C2C12 myoblasts into fully differentiated post-mitotic myotubes. The SMO gene induction represents a novel and additional marker of C2C12 cell differentiation. The sub-cellular localization of the SMOalpha and SMOmu splice variants is not involved in the differentiation processes. Nuclear localization of only the SMOmu protein was confirmed.
Dipartimento di Biologia, Università Roma Tre, Rome, Italy.
Manuela
M
Cervelli
Emiliano
E
Fratini
Roberto
R
Amendola
Marzia
M
Bianchi
Emanuela
E
Signori
Elisabetta
E
Ferraro
Antonella
A
Lisi
Rodolfo
R
Federico
Lucia
L
Marcocci
Paolo
P
Mariottini
D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D007527:Isoenzymes; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000201:enzymology*; D032446:Myoblasts / Q000166:cytology / Q000201:enzymology*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism; D011132:Polyribosomes / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D000097667:Polyamine Oxidase
D007527:Isoenzymes; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D000097667:Polyamine Oxidase; C026452:messenger ribonucleoprotein