Assessing methods for blood cell cytotoxic responses to inorganic nanoparticles and nanoparticle aggregates.
Inorganic nanoparticles (NPs) show great potential for medicinal therapy. However, biocompatibility studies are essential to determine if they are safe. Here, five different NPs are compared for their cytotoxicity, internalization, aggregation in medium, and reactive oxygen species (ROS) production, using tumoral and normal human blood cells. Differences depending on the cell type are analyzed, and no direct correlation between ROS production and cell toxicity is found. Results are discussed with the aim of standardizing the procedures for the evaluation of the toxicity.
Faculty of Biology, University of Vigo, Vigo, Pontevedra, Spain.
Belén
B
Díaz
Christian
C
Sánchez-Espinel
Manuel
M
Arruebo
José
J
Faro
Encarnación
E
de Miguel
Susana
S
Magadán
Clara
C
Yagüe
Rodrigo
R
Fernández-Pacheco
M Ricardo
MR
Ibarra
Jesús
J
Santamaría
Africa
A
González-Fernández
D000818:Animals; D001773:Blood Cells / Q000166:cytology* / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D018384:Oxidative Stress / Q000502:physiology; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species