Improvement of viral recombinant protein-based immunoassays using nanostructured hybrids as solid support.
Protein adsorption onto solid surfaces is a complex process playing an important role in biological systems, being crucial to maintain the three-dimensional conformation during molecules interaction. Polymers hybrid network could be used to perform immunoassays with the required high level specificity. We have developed a novel route for incorporating recombinant protein from Bovine Herpesvirus into a network using as template Poly(vinyl alcohol) chemically tailored with five organosilanes followed by FTIR spectroscopy characterization. Protein expressed in E. coli was incorporated into hybrid network; all the PVA-hybrids interacted with this biomolecule and FTIR vibrational bands have endorsed the results. PVA hybrids modified with 3-(triethoxysilyl) propylisocyanate-TESPI presented the best result in the immunoassay, enhancing the sera specificity in comparison with the commercial microplate. We have successfully developed organic-inorganic hybrid based on PVA-silane to be used in serological tests with recombinant protein as a promising tool for the development and production new diagnostic assays.
Mansur
Herman S
HS
Departamento de Engenharia Metalúrgica e de Materiais Escola de Engenharia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Palhares
Rafael M
RM
Andrade
Giovanna I
GI
Piscitelli Mansur
Alexandra A
AA
Barbosa-Stancioli
Edel Figueiredo
EF
D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics
D020099:Coated Materials, Biocompatible; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C044200:glycoprotein H, herpes simplex virus type 1