MicroRNA profiling of megakaryocytes.
MiRNAs are non-coding RNAs of 19-25 nucleotides in length that regulate gene expression. Recent work suggests a role for miRNAs during hematopoiesis. Here, we described in detail how to successfully obtain highly pure megakaryocytes precursors from human CD34+ stem cells in culture to perform high-throughput miRNA profiling using a custom microarray platform.
Department of Medicine Division of Hematology and Oncology, Comprehensive Cancer Center, The Ohio State University, USA.
Ramiro
R
Garzon
D000818:Animals; D018952:Antigens, CD34; D002478:Cells, Cultured; D020869:Gene Expression Profiling / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008533:Megakaryocytes / Q000378:metabolism*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
D018952:Antigens, CD34; D035683:MicroRNAs