Solid-phase synthesis and evaluation of TAR RNA targeted beta-carboline-nucleoside conjugates.
Four types of beta-carboline-nucleoside conjugates were synthesized. The binding affinities of these beta-carboline-nucleoside conjugates , and to TAR RNA were evaluated by affinity capillary electrophoresis. The data of binding affinities to TAR RNA show that conjugates and are stronger binders than the parent compound . Computer modeling indicates that the beta-carboline-nucleoside conjugate can fit to the UCU three-nucleotide bulge region of TAR RNA.
State Key Laboratory of Natural & Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100083, China.
Peng
P
Zhao
Hong-Wei
HW
Jin
Zhen-Jun
ZJ
Yang
Liang-Ren
LR
Zhang
Li-He
LH
Zhang
D001483:Base Sequence; D002243:Carbolines / Q000378:metabolism*; D019075:Electrophoresis, Capillary; D016325:HIV Long Terminal Repeat / Q000235:genetics*; D008958:Models, Molecular; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism*; D013379:Substrate Specificity; D015533:Transcriptional Activation / Q000235:genetics*
D002243:Carbolines; D009705:Nucleosides; D012367:RNA, Viral