Microbial production and characterization of superparamagnetic magnetite nanoparticles by Shewanella sp. HN-41.
A facultative dissimilatory metal-reducing bacterium, Shewanella sp. strain HN-41, was used to produce magnetite nanoparticles from a precursor, poorly crystalline ironoxyhydroxide akaganeite (beta-FeOOH), by reducing Fe(III). The diameter of the biogenic magnetite nanoparticles ranged from 26 nm to 38 nm, characterized by dynamic light scattering spectrophotometry. The magnetite nanoparticles consisted of mostly uniformly shaped spheres, which were identified by electron microscopy. The magnetometry revealed the superparamagnetic property of the magnetic nanoparticles. The atomic structure of the biogenic magnetite, which was determined by extended X-ray absorption fine structure spectroscopic analysis, showed similar atomic structural parameters, such as atomic distances and coordinations, to typical magnetite mineral.
Department of Environmental Science and Engineering, and International Environmental Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
Ji-Hoon
JH
Lee
Yul
Y
Roh
Hor-Gil
HG
Hur
D005290:Ferric Compounds / Q000378:metabolism; D052203:Ferrosoferric Oxide / Q000737:chemistry* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D020592:Shewanella / Q000378:metabolism*; D013053:Spectrophotometry
D005290:Ferric Compounds; C473845:akaganeite; D052203:Ferrosoferric Oxide