[Preliminary study of effects and mechanisms of qingpeng paste on type II collagen-induced arthritis in rats].
OBJECTIVE
To investigate the effect of Qingpeng paste (QP) on collagen-induced arthritis (CIA) in rats.


METHODS
CIA was established in female Wistar rats with injection of type II bovine collagen at the base of the tail of animals. CIA rats were treated daily with external administration of different doses of QP or voltaren beginning on the day after the onset of arthritis (day 1) until day 20. Paw swelling rate and the serum levels of IL-1 beta were determined. Moreover, the expression of TNF-alpha and IL-alpha and histopathological changes in the arthritic joints were also observed.


RESULTS
QP markedly suppressed the paw swelling rate of arthritic rat, reduced the expression of TNF-alpha and IL-alpha in synovial membrane. Histopathological changes in the arthritic joints were also significantly ameliorated in the QP-treated versus vehicle-treated rats. However, the elevated serum levels of IL-1 beta in arthritic rats were not influenced by QP.


CONCLUSIONS
The present findings demonstrate the protective property of QP on collagen-induced arthritis, mechanisms underlying it may be related to reduce the expression of IL-1alpha and TNF-alpha in synovial membrane.
Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100083, China.
Chao-yong
CY
He
Nan
N
Li
Jie
J
Ma
Li-hua
LH
Chen
Wei-dong
WD
Li
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000139:chemically induced* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D024043:Collagen Type II / Q000009:adverse effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D053582:Interleukin-1alpha / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013583:Synovial Membrane / Q000187:drug effects / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D024043:Collagen Type II; D004365:Drugs, Chinese Herbal; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; C540321:Qingpeng plaster; D014409:Tumor Necrosis Factor-alpha