Brucella abortus strain RB51 leucine auxotroph as an environmentally safe vaccine for plasmid maintenance and antigen overexpression.
To avoid potentiating the spread of an antibiotic resistance marker, a plasmid expressing a leuB gene and a heterologous antigen, green fluorescent protein (GFP), was shown to complement a leucine auxotroph of cattle vaccine strain Brucella abortus RB51, which protected CD1 mice from virulent B. abortus 2308 and elicited GFP antibodies.
Department of Biomedical Sciences and Pathobiology and Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0342, USA.
Parthiban
P
Rajasekaran
Mohamed N
MN
Seleem
Andrea
A
Contreras
Endang
E
Purwantini
Gerhardt G
GG
Schurig
Nammalwar
N
Sriranganathan
Stephen M
SM
Boyle
D000818:Animals; D002004:Brucella Vaccine / Q000235:genetics* / Q000276:immunology*; D002003:Brucella abortus / Q000235:genetics* / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control*; D018628:Gene Dosage; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D007930:Leucine / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D010957:Plasmids
D002004:Brucella Vaccine; D049452:Green Fluorescent Proteins; D007930:Leucine