Circular dichroism reveals sensitivity of glucagon solution structure to fluoroalcohols, pH and ionic strength.
Circular dichroism reports that glucagon in solution becomes increasingly helical with the addition of fluoroalcohol (which also decreases solution pH), and with changes in pH and ionic strength. Given the variability of structure observed, these data indicate that care must be taken when comparing results obtained under different solution conditions.
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Centre for Biologics Research, Biologics and Genetic Therapies Directorate, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa ON, Canada.
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D000438:Alcohols / Q000737:chemistry* / Q000494:pharmacology*; D002942:Circular Dichroism; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D020005:Propanols / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D012680:Sensitivity and Specificity; D012996:Solutions; D012997:Solvents / Q000494:pharmacology; D014270:Trifluoroethanol / Q000494:pharmacology; D006845:Hydrocarbons, Fluorinated
D000438:Alcohols; D005934:Glucagon; D020005:Propanols; D012996:Solutions; D012997:Solvents; D014270:Trifluoroethanol; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated