The relaxant effect of okadaic acid on canine basilar artery involves activation of PKCalpha and phosphorylation of the myosin light chain at Thr-9.
Vasodilator responses induced by okadaic acid were investigated in canine basilar artery precontracted with 80 mM KCl. Okadaic acid (1 microM) relaxed the artery and this relaxant effect was partially inhibited by Gö6976, a conventional protein kinase C inhibitor, and calphostin C, an inhibitor of conventional and novel PKCs. Rottlerin, a specific inhibitor of PKCdelta, did not influence okadaic acid's effect. KCl increased phosphorylation of 20,000-Dalton myosin light chain (MLC(20)) at Ser-19. Okadaic acid additionally increased MLC(20) phosphorylation at Thr-18 and Thr-9, resulting in triphosphorylation of MLC(20). This phosphorylation was inhibited by Gö6976. Okadaic acid stimulated phosphorylation of PKCalpha and 17,000-Dalton PKC-potentiated inhibitory phosphoprotein (CPI-17), and Gö6976 inhibited these phosphorylations. These results suggest that okadaic acid's relaxant effect involves MLC(20) triphosphorylation through a direct phosphorylation by PKCalpha and an indirect phosphorylation by inhibition of myosin light chain phosphatase through PKCalpha-mediated CPI-17 phosphorylation.
Obara
Kazuo
K
Department of Pharmacology, Graduate School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka-City, Shizuoka 422-8526, Japan. obara@u-shizuoka-ken.ac.jp
Ito
Yoshihiko
Y
Shimada
Hiroki
H
Nakayama
Koichi
K
D000818:Animals; D001488:Basilar Artery / Q000187:drug effects*; D015153:Blotting, Western; D002227:Carbazoles / Q000494:pharmacology; D004285:Dogs; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D007537:Isometric Contraction / Q000187:drug effects; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D018994:Myosin Light Chains / Q000378:metabolism*; D009281:Naphthalenes / Q000494:pharmacology; D019319:Okadaic Acid / Q000494:pharmacology*; D010449:Peptide Mapping; D010766:Phosphorylation; D011189:Potassium Chloride / Q000494:pharmacology; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism*
D002227:Carbazoles; D004791:Enzyme Inhibitors; D018994:Myosin Light Chains; D009281:Naphthalenes; C081021:Go 6976; D019319:Okadaic Acid; D013912:Threonine; D011189:Potassium Chloride; D051571:Protein Kinase C-alpha; C058819:calphostin C