[Reversal effect of aloe emodin liposomes on cisplatin resistance line A549/DDP human lung adenocarcinoma cells].
OBJECTIVE
To determine the reversal effect of aloe emodin liposomes (AE-L) on cisplatin resistance human lung adenocarcinoma cell line A549/DDP.


METHODS
The un-cytotoxic and cytotoxic concentration of AE-L, and un-cytotoxic concentration of E-L were determined by MTT. The sensitivity of cisplatin were determined by MTT assay in above 3 groups. The intracellular concentration of cisplatin was detected by inductively coupled plasma mass spectrometry (ICP-MS).


RESULTS
The maximum non-toxic concentration group of AE-L (2.0 mg x L(-1)) increased the sensitivity of cisplatin in A549/DDP, decreased IC50 of cisplatin in A549/DDP from 16.81 mg x L(-1) to 5.86 mg x L(-1), and the hyp-cytotoxic concentration (7.0 mg x L(-1) ) group's IC50 decreased to 4.34 mg x L(-1); AE-L groups significantly increased intracellular concentration of cisplatin in A549/DDP cells.


CONCLUSIONS
The results showed that aloe emodin can reverse multidrug resistance (MDR) of A549/DDP cells and the mechanism might be associated with the increase of intracellular concentration of cisplatin.
Dept of Pharmacology, Hunan University of Traditional Chinese Medicine, Changsha 410007, China.
Rong
R
Zeng
Zhi-wu
ZW
Zhou
Cui-fang
CF
Wu
Yu-lu
YL
Zhou
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D000880:Anthraquinones / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D007928:Lethal Dose 50; D008081:Liposomes; D008175:Lung Neoplasms / Q000473:pathology
D000880:Anthraquinones; D008081:Liposomes; C518327:aloe emodin; D002945:Cisplatin