Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China.
Two Gram-positive, motile, rod-shaped, halophilic bacteria showing optimum growth at 5-7 % (w/v) NaCl were isolated from a salt lake, Xin-Jiang Province, China. The strains, designated BH312(T) and BH314, grew in the presence of 1-18 % (w/v) NaCl and at temperatures of 15-50 degrees C (optimum: 40 degrees C) and pH values of 5.5-10.0 (optimum: pH 7.5-8.0). The major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 38.8-39.0 mol% and the predominant lipoquinone was MK-7. The major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates formed a cluster with Gracilibacillus orientalis XH-63(T) within the genus Gracilibacillus. The levels of 16S rRNA gene sequence similarity with closely related type strains were lower than 96.2 %. On the basis of phylogenetic, phenotypic and chemotaxonomic properties, the isolates represent a novel species of the genus Gracilibacillus for which the name Gracilibacillus lacisalsi sp. nov. is proposed. The type strain is BH312(T) (=KCTC 13129(T)=DSM 19029(T)).
Jeon
Che Ok
CO
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Lim
Jee-Min
JM
Jang
Ho Hee
HH
Park
Dong-Jin
DJ
Xu
Li-Hua
LH
Jiang
Cheng-Lin
CL
Kim
Chang-Jin
CJ
D001406:Bacillaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride