Relationship between CD107a expression and cytotoxic activity.
NK cells play important roles in innate immunity against tumors and infections of the host. Studies show that CD107a (LAMP-1) may be a marker for degranulation of NK and activated CD8+ T cells. In our study, the relationship between the expression of CD107a, cytokine secretion and cytotoxic activity in CD56+ NK, CD8+ T cells and lymphocytes has been determined after various stimuli. Effector cells from PBMCs of healthy subjects were isolated and K562 cell line was used as target of cytotoxicity. IL-2 stimulation resulted in a significant increase of CD107a expression in CD56+ NK, CD8+ T cells and lymphocytes. Increased expression of CD107a after IL-2 stimulation of NK cells was parallel to the increase of cytotoxicity. Our results suggest that CD107a expression may be a sensitive marker for the cytotoxic activity determination.
Aktas
Esin
E
Department of Immunology, Institute of Experimental Medicine (DETAE), Istanbul University, Vakif Gureba Caddesi, Sehremini, 34393 Istanbul, Turkey.
Kucuksezer
Umut Can
UC
Bilgic
Sema
S
Erten
Gaye
G
Deniz
Gunnur
G
D000328:Adult; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000378:metabolism; D052118:Lysosomal-Associated Membrane Protein 1 / Q000235:genetics / Q000276:immunology*; D008297:Male; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
D007376:Interleukin-2; D052118:Lysosomal-Associated Membrane Protein 1; D016753:Interleukin-10