Propionate sensor using coenzyme-A transferase and acyl-CoA oxidase.
We developed an amperometric propionate sensor using comprised of two recombinant enzymes, propionate coenzyme A CoA transferase from Clostridium propionicum and short-chain acyl-CoA oxidase from Arabidopsis thaliana. Response current increased linearly with increase in propionate concentration from 10 microM to 100 microM. The detection limit was 10 microM propionate.
Sode
Koji
K
Department of Biotechnology, Tokyo University of Agriculture and Technology, Naka-cho, Koganei, Tokyo 184-8588 Japan. sode@cc.tuat.ac.jp
Tsugawa
Wakako
W
Aoyagi
Minoru
M
Rajashekhara
Eranna
E
Watanabe
Kazuya
K
D042962:Acyl-CoA Oxidase / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology*; D015374:Biosensing Techniques / Q000379:methods*; D003013:Clostridium / Q000201:enzymology*; D015256:Coenzyme A-Transferases / Q000378:metabolism*; D004563:Electrochemistry; D011422:Propionates / Q000378:metabolism*
D011422:Propionates; D042962:Acyl-CoA Oxidase; D015256:Coenzyme A-Transferases; C031212:acyl-CoA transferase