Enhancing the biocatalytic potential of carbonyl reductase of Candida viswanathii using aqueous-organic solvent system.
In the present work, the toxic effect of various solvents with different Log P values was studied on the whole cells of Candida viswanathii. Experiments showed that the lower concentrations of some solvent increased both the activity retention and enzyme activity as compared to the control while this was not the case with higher concentrations of the same solvents. The model compound taken in the present study was 1-acetophenone. The percentage conversion improved from 76 to 94%. Addition of 2-propanol increased the substrate tolerance, giving the conversion of 90% compared to 9% in control at a substrate concentration of 70 mM in 1h. The operational stability increased at higher temperatures with the addition of 2-propanol in the reaction mixture with good conversion (90%) and enantiomeric excess (>99%) at 45 degrees C and 50 degrees C. The effect was also found to be prominent in other tested substrates. In order to further stabilize the cells for long term use in higher concentration of organic solvents, the cells were further immobilized, and were found to have higher activity retention than that of free cells.
Department of Pharmaceutical Technology, Biocatalysis Laboratory, National Institute of Pharmaceutical Education and Research, Mohali, Punjab, India.
Himani
H
Kansal
Uttam Chand
UC
Banerjee
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D002175:Candida / Q000145:classification* / Q000201:enzymology*; D002384:Catalysis; D002470:Cell Survival; D018914:Cells, Immobilized / Q000187:drug effects; D004789:Enzyme Activation; D004795:Enzyme Stability; D009930:Organic Chemicals / Q000737:chemistry* / Q000494:pharmacology*; D012997:Solvents / Q000737:chemistry / Q000494:pharmacology; D014867:Water / Q000378:metabolism*
D009930:Organic Chemicals; D012997:Solvents; D014867:Water; D000429:Alcohol Oxidoreductases