Isolation of Toxoplasma gondii from goats from Brazil.
Goats are economically important in many countries, and little is known of caprine toxoplasmosis in Brazil. Antibodies to Toxoplasma gondii were assayed in the sera of 143 goats from 3 Brazilian states, using modified agglutination test (MAT titer > or = 1:25); 46 (32.2%) tested positive. Samples of brain, heart, diaphragm, and masseter of seropositive animals were pooled, digested in pepsin, and bioassayed in mice. Viable T. gondii specimens were isolated from tissue homogenates of 12 goats; the isolates were designated TgGtBr1-12. Ten of the 12 isolates killed 100% of infected mice, indicating that goats can harbor mouse-virulent T. gondii and, hence, can serve as a source of infection for humans.
Department of Preventive Veterinary Medicine and Animal Health, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil.
A M A
AM
Ragozo
L E O
LE
Yai
L N
LN
Oliveira
R A
RA
Dias
H C
HC
Gonçalves
S S
SS
Azevedo
J P
JP
Dubey
S M
SM
Gennari
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001681:Biological Assay / Q000662:veterinary; D001938:Brazil / Q000453:epidemiology; D005260:Female; D015511:Goat Diseases / Q000453:epidemiology / Q000469:parasitology*; D006041:Goats; D008297:Male; D051379:Mice; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology / Q000469:parasitology*
D000913:Antibodies, Protozoan