Exploring the electron transfer properties of neuronal nitric-oxide synthase by reversal of the FMN redox potential.
In nitric-oxide synthase (NOS) the FMN can exist as the fully oxidized (ox), the one-electron reduced semiquinone (sq), or the two-electron fully reduced hydroquinone (hq). In NOS and microsomal cytochrome P450 reductase the sq/hq redox potential is lower than that of the ox/sq couple, and hence it is the hq form of FMN that delivers electrons to the heme. Like NOS, cytochrome P450BM3 has the FAD/FMN reductase fused to the C-terminal end of the heme domain, but in P450BM3 the ox/sq and sq/hq redox couples are reversed, so it is the sq that transfers electrons to the heme. This difference is due to an extra Gly residue found in the FMN binding loop in NOS compared with P450BM3. We have deleted residue Gly-810 from the FMN binding loop in neuronal NOS (nNOS) to give Delta G810 so that the shorter binding loop mimics that in cytochrome P450BM3. As expected, the ox/sq redox potential now is lower than the sq/hq couple. Delta G810 exhibits lower NO synthase activity but normal levels of cytochrome c reductase activity. However, unlike the wild-type enzyme, the cytochrome c reductase activity of Delta G810 is insensitive to calmodulin binding. In addition, calmodulin binding to Delta G810 does not result in a large increase in FMN fluorescence as in wild-type nNOS. These results indicate that the FMN domain in Delta G810 is locked in a unique conformation that is no longer sensitive to calmodulin binding and resembles the "on" output state of the calmodulin-bound wild-type nNOS with respect to the cytochrome c reduction activity.
Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA.
Huiying
H
Li
Aditi
A
Das
Hiruy
H
Sibhatu
Joumana
J
Jamal
Stephen G
SG
Sligar
Thomas L
TL
Poulos
D000818:Animals; D001671:Biochemistry / Q000379:methods; D002147:Calmodulin / Q000378:metabolism; D005486:Flavin Mononucleotide / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D008968:Molecular Conformation; D009251:NADPH-Ferrihemoprotein Reductase / Q000737:chemistry; D009474:Neurons / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D010084:Oxidation-Reduction; D011485:Protein Binding; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors
D002147:Calmodulin; D005486:Flavin Mononucleotide; D052248:Nitric Oxide Synthase Type I; D009251:NADPH-Ferrihemoprotein Reductase