Immunochip for the detection of five kinds of chemicals: Atrazine, nonylphenol, 17-beta estradiol, paraverine and chloramphenicol.
This paper reports an immunochip for the detection of five kinds of chemicals: atrazine, nonylphenol, 17-beta estradiol, paraverine and chloramphenicol, which are harmful to human health and environment due to unconscionable use or abuse. Five antigen conjugates were synthesized, purified and then labeled with Cy3 dye. Meanwhile, the immunochip was prepared by immobilization the five kinds of specific antibodies on aldehydized slides as probes. A series of experimental conditions were optimized and selected. Standard curves were plotted and the detection ranges were 0.001-5 microg/mL with good logistic correlation and linear correlation (R(2)>0.99). All the five chemicals can be simultaneously and quantitatively determined in a chip, and the relative standard deviations were within 10%. The immunochip will become a highly sensitive, rapid, alternative analytical tool for detection of chemicals or toxicants.
Gao
Zhixian
Z
Institute of Hygiene & Environmental Medicine, Tianjin 300050, PR China.
Liu
Nan
N
Cao
Qiaoling
Q
Zhang
Lei
L
Wang
Shengqi
S
Yao
Wei
W
Chao
Fuhuan
F
D001280:Atrazine / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002701:Chloramphenicol / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004958:Estradiol / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D010636:Phenols / Q000032:analysis*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D010636:Phenols; D004958:Estradiol; D002701:Chloramphenicol; C025256:nonylphenol; D001280:Atrazine