Correlation between cell cycle phase of adherent Chinese hamster ovary cells and laser phase shift determined by phase-shifting laser microscopy.
The simultaneous determination of the cell cycle phase of individual adherent Chinese hamster ovary cells using a fluorescence microscope after staining with 4',6-diamidine-2'-phenylindole dihydrochloride and bromodeoxyuridine and the laser phase shift by a phase-shifting laser microscopy revealed that the laser phase shift of cells in the G2/M phase was markedly higher than that of cells in the G1 and S phases.
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan.
Syunsuke
S
Ito
Mutsumi
M
Takagi
D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D002453:Cell Cycle; D006224:Cricetinae; D003412:Cricetulus; D007834:Lasers; D018613:Microscopy, Confocal / Q000379:methods*