Modified multiplex PCR methods for comprehensive detection of Pectinatus and beer-spoilage cocci.
Specific PCR primers were designed based on the 16S rRNA genes of recently proposed beer-spoilage species, Pectinatus haikarae, Megasphaera sueciensis, and M. paucivorans, and two sets of our previously reported multiplex PCR methods for Pectinatus spp. and beer-spoilage cocci were reconstructed. Each modified multiplex PCR method was found specifically to detect beer-spoilage species of Pectinatus and cocci, including new species.
Research Laboratories of Brewing Technology, Asahi Breweries Ltd., Ibaraki, Japan. kazumaru.iijima@asahibeer.co.jp
Kazumaru
K
Iijima
Shizuka
S
Asano
Koji
K
Suzuki
Tomoo
T
Ogata
Yasushi
Y
Kitagawa
D001515:Beer; D045851:Megasphaera / Q000235:genetics*; D045852:Pectinatus / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity