Transcription expression and clinical significance of TTF-1 mRNA in pleural effusion of patients with lung cancer.
The aim of this study was to evaluate diagnostic utility of thyroid transcription factor 1 (TTF-1) mRNA in pleural effusions (PEs) of patients with lung cancer, especially primary pulmonary adenocarcinoma (PPA). Transcription levels of TTF-1 were detected by reverse transcription polymerase chain reaction (RT-PCR) in PEs of patients with lung cancer (56 cases) and with lung benign diseases (44 cases). The expression rate of TTF-1 mRNA was significantly higher in PEs of patients with lung cancer (73.2%) than with benign lung diseases (0%). In subclasses, the expression rate of TTF-1 mRNA was obviously higher in PEs of patients with PPA (93.0%) than with metastatic pulmonary adenocarcinoma (0%) and with primary pulmonary squamous cell carcinoma (12.5%). When compared with immunocytochemistry and cytology, TTF-1 mRNA gave the best diagnostic performance in diagnosing PPA: sensitivity of 93.0%, specificity of 100%, and accuracy of 96.6%, respectively. As a molecular marker of detecting pleural micrometastasis, TTF-1 mRNA may be helpful to diagnose the cancer cells in PEs of patients with lung cancer, especially with PPA.
Jiang
Bing
B
Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang 110001, China.
Wu
Guang-Ping
GP
Zhao
Yu-Jie
YJ
Wang
Shao-Cheng
SC
D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D016066:Pleural Effusion, Malignant / Q000175:diagnosis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012680:Sensitivity and Specificity; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D014408:Biomarkers, Tumor; C000614412:NKX2-1 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors