IgA response by oral infection with an attenuated Yersinia enterocolitica mutant: implications for its use as oral carrier vaccine.
Yersinia enterocolitica (Ye) mutant strain (sycH-) is unable to secrete the virulence protein YopH. Mucosal vaccination is often required to induce protection, but stimulating strong IgA response is frequently difficult. Here, we addressed whether Ye sycH- might induce IgA response, and investigated its attenuation in TNFRp55-/-, IL-12p40-/- and IL-4-/- mice. We found that Ye sycH- colonizes Peyer's patches, and induces higher Yersinia-specific IgA levels in feces and in serum compared with Ye wild type. The Ye sycH-mutant proved to be attenuated and induced IgA in both wild-type and immunodeficient mice. These lines of evidence show the attenuation of Ye sycH- and its ability to stimulate an IgA response. This mutant might be useful as an oral vaccine carrier.
Blanco
Helga M
HM
Immunology, Laboratory of Microbiology, Chemistry, Biochemistry and Pharmacy Faculty, National University of San Luis, IMIBIO-SL, CONICET, San Luis, Argentina.
Lacoste
María G
MG
Eliçabe
Ricardo J
RJ
Di Genaro
María S
MS
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D001428:Bacterial Vaccines; D005243:Feces / Q000382:microbiology; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000097:blood* / Q000276:immunology; D015847:Interleukin-4 / Q000172:deficiency; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation; D010581:Peyer's Patches / Q000276:immunology / Q000382:microbiology; D015009:Yersinia Infections / Q000276:immunology*; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D015847:Interleukin-4