Synthesis, crystal structure and interaction with DNA and HSA of (N,N'-dibenzylethane-1,2-diamine) transition metal complexes.
A new ligand, N,N'-dibenzylethane-1,2-diamine (L) and its four transition metal(II) complexes, ML(2)(OAc)(2).2H(2)O (M=Cu, Ni, Zn, Co), have been synthesized and characterized by elemental analysis, mass spectra, molar conductivity, NMR and IR. Moreover, the crystals structure of Cu(II) and Ni(II) complexes characterized by single crystal X-ray diffraction showed that the complexes have a similar molecular structure. Ni(II) has an regular octahedral coordination environment complexes, but typical Jahn Teller effect influenced Cu(II) in an elongated octahedral environment. The interaction between complexes and calf thymus DNA were studied by UV and fluorescence spectra measure, which showed that the binding mode of complexes with DNA is intercalation. Under physiological pH condition, the effects of Cu(OAc)(2)L(2).2H(2)O and Ni(OAc)(2)L(2).2H(2)O on human serum albumin were examined by fluorescence. The results of spectroscopic measurements suggested that the hydrophobic interaction is the predominant intermolecular force. The enthalpy change DeltaH(0) and the entropy change DeltaS(0) of Cu(OAc)(2)L(2).2H(2)O and Ni(OAc)(2)L(2).2H(2)O were calculated to be -11.533 kJ mol(-1) and 46.339 J mol(-1)K(-1), -11.026 kJ mol(-1) and 46.396 J mol(-1)K(-1), respectively, according to the Scatchard's equation. The quenching mechanism and the number of binding site (n approximately 1) were also obtained from fluorescence titration data.
Key Laboratory of Tropic Biological Resources of Ministry of Education, Hainan Provincial Key Laboratory of Fine Chemical, Hainan University, Renmin Avenue 58, Haikou 570228, China.
Shan-Shan
SS
Wu
Wen-Bing
WB
Yuan
Hai-Yang
HY
Wang
Qi
Q
Zhang
Min
M
Liu
Kai-Bei
KB
Yu
D001665:Binding Sites; D003035:Cobalt / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D003959:Diamines / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D009532:Nickel / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D028561:Transition Elements / Q000737:chemistry; D014961:X-Ray Diffraction; D015032:Zinc / Q000737:chemistry
D003959:Diamines; D007364:Intercalating Agents; D008024:Ligands; D009942:Organometallic Compounds; D012709:Serum Albumin; D028561:Transition Elements; D003035:Cobalt; D003300:Copper; D009532:Nickel; D004247:DNA; C427203:calf thymus DNA; D015032:Zinc