PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria.
We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the P(BAD) promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.
Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine at Marshall University, Huntington, West Virginia 25755-9320, USA.
Dongru
D
Qiu
F Heath
FH
Damron
Takehiko
T
Mima
Herbert P
HP
Schweizer
Hongwei D
HD
Yu
D051777:AraC Transcription Factor / Q000235:genetics; D019117:Burkholderia / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000235:genetics*
D051777:AraC Transcription Factor; C497371:AraC protein, E coli; D029968:Escherichia coli Proteins