[Preliminary study on herpes simplex virus type 1 infection of human oral epithelial cell in vitro].
OBJECTIVE
To investigate the way of herpes simplex virus type 1 (HSV-1) infecting human oral epithelial cell in vitro.


METHODS
Abundance of HSV-1 strains were used to infect human oral epithelial cells. The culture supernatant was collected to infect Vero cells again. Morphology of HSV-1 was identified by inverted microscope and transmission electron microscope. Nucleic acid of the virus was detected by PCR.


RESULTS
The infected human oral epithelial cells didn't display obvious cytopathic effect (CPE) while Vero cells infected by the culture supernatant showed typical CPE. The virus particles were observed in Vero cells. The nucleic acid of HSV-1 could be detected in infected human oral epithelial cells by PCR.


CONCLUSIONS
HSV-1 could successfully infect human oral epithelial cells.
Department of Periodontology, Stomatological College of Nanjing Medical University, Nanjing 210029, China.
Jie
J
Zhao
Wei-Bin
WB
Sun
Juan
J
Wang
D000818:Animals; D002522:Chlorocebus aethiops; D004847:Epithelial Cells / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D006801:Humans; D009055:Mouth / Q000166:cytology; D014709:Vero Cells