The divided aperture technique for microscopy through scattering media.
A diffraction analysis is presented for image formation in confocal microscopy using the divided aperture technique, which uses two D-shaped apertures (also called specular microscopy). The effects of increasing the width of a divider, that separates the two D shapes, are investigated. As the width is increased, the resolution degrades. The efficiency of singly-scattered light rejection is not improved with increased width.
Division of Bioengineering, National University of Singapore, Singapore. colin@nus.edu.sg
Colin J
CJ
Sheppard
Wei
W
Gong
Ke
K
Si
D003198:Computer Simulation; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008027:Light; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008962:Models, Theoretical; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation*; D012542:Scattering, Radiation