New insights into SRY regulation through identification of 5' conserved sequences.
BACKGROUND
SRY is the pivotal gene initiating male sex determination in most mammals, but how its expression is regulated is still not understood. In this study we derived novel SRY 5' flanking genomic sequence data from bovine and caprine genomic BAC clones.


RESULTS
We identified four intervals of high homology upstream of SRY by comparison of human, bovine, pig, goat and mouse genomic sequences. These conserved regions contain putative binding sites for a large number of known transcription factor families, including several that have been implicated previously in sex determination and early gonadal development.


CONCLUSIONS
Our results reveal potentially important SRY regulatory elements, mutations in which might underlie cases of idiopathic human XY sex reversal.
Division of Molecular Genetics and Development, Institute for Molecular Bioscience, The University of Queensland, Brisbane 4072, Australia. d.farkas@imb.uq.edu.au
Diana G F
DG
Ross
Josephine
J
Bowles
Peter
P
Koopman
Sigrid
S
Lehnert
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002417:Cattle; D017124:Conserved Sequence / Q000235:genetics*; D004247:DNA; D024282:Genes, sry; D006041:Goats; D006801:Humans; D008297:Male; D051379:Mice; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D016415:Sequence Alignment / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D051876:Sex-Determining Region Y Protein / Q000235:genetics*; D013552:Swine; D014157:Transcription Factors / Q000235:genetics
D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D004247:DNA