Oxidative status and DNA damage in operating room personnel.
OBJECTIVE
The aim of this study was to investigate the effects of occupational exposure to anesthetic gases on peripheral mononuclear leukocyte DNA damage and oxidative status in operating room personnel.


METHODS
The population of study includes 30 operating room personnel (Group I) and 30 non-operating room personnel for control groups (Group II). DNA damage was assessed by alkaline comet assay in peripheral lymphocyte. Plasma levels of total antioxidant status (TAS), total oxidative status (TOS) were determined by using a novel automated measurement method and oxidative stress index (OSI) was calculated (OSI was calculated as: OSI=(TOS/TAS)x100).


RESULTS
Mononuclear leukocyte DNA damage, plasma TOS and OSI were significantly higher in Group I than Group II [p<0.01, p<0.05, p<0.05 respectively]. There was also significantly decrease in plasma TAS in Group I than Group II [p<0.05]. There were positive significant correlations between DNA damage and TOS and OSI in Group I [r=0.84, p<0.001, r=0.89, p<0.001 respectively].


CONCLUSIONS
Our findings demonstrate that, mononuclear leukocyte DNA damage was significantly higher in operating room personnel. The increase of DNA damage might depend on oxidative effects of anesthetics.
Baysal
Zeynep
Z
Harran University, Faculty of Medicine, Department of Anesthesiology and Reanimation, Sanliurfa, Turkey. zbaysal2003@yahoo.com
Cengiz
Mustafa
M
Ozgonul
Abdullah
A
Cakir
Muslum
M
Celik
Hakim
H
Kocyigit
Abdurrahim
A
D000328:Adult; D000777:Anesthetics / Q000009:adverse effects; D016022:Case-Control Studies; D004249:DNA Damage; D006801:Humans; D007963:Leukocytes, Mononuclear; D016273:Occupational Exposure; D009873:Operating Rooms; D018384:Oxidative Stress; D010564:Personnel, Hospital
D000777:Anesthetics