Triphasic ceramic coated hydroxyapatite as a niche for goat stem cell-derived osteoblasts for bone regeneration and repair.
Current treatment strategies for the repair or replacement of bone use synthetic implants with stem cells and their progeny--a new approach to address unmet medical needs. This study has evaluated the effect of a silica-coated bioactive ceramic, namely HASi in comparison to hydroxyapatite (HA) on the adhesion, proliferation and osteogenic differentiation of goat bone marrow-derived mesenchymal stem cells in vitro in a prolonged culture of 28 days. The cellular activities were significantly enhanced on HASi signifying the role of silica to stimulate osteoblast cells. The fabrication of such a 'cell-ceramic construct using autologous MSCs' is aimed for the transplantation to a large bone defect site in the goat femur model which still remains a formidable challenge in Orthopedic surgery.
Transmission Electron Microscopy Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Trivandrum 695 012, India.
Manitha B
MB
Nair
H K
HK
Varma
Annie
A
John
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000138:chemical synthesis / Q000737:chemistry; D002454:Cell Differentiation; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D017102:Fracture Healing / Q000502:physiology*; D006041:Goats; D016348:Osseointegration / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D016062:Porosity; D013234:Stem Cells / Q000502:physiology*; D054457:Tissue Scaffolds
D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D017886:Durapatite