Effects of anti-phospholipid antibodies on a human trophoblast cell line (HTR-8/SVneo).
Antibodies to phospholipids (aPL) have been shown to adversely affect trophoblast invasion in vivo and in vitro. HTR-8/SVneo cells derived from first trimester of pregnancy extravillous trophoblast were studied. Matrigel invasion assay, cytochemistry and cell-based enzyme-linked immunosorbant assay (ELISA) with aPL or normal IgG was used. Our data show that aPL at 100 microg/ml decrease invasiveness of HTR-8/SVneo cells to 60% of control (p<0.01), and this was also shown for primary cytotrophoblast (to 15.5% of control, p<0.001). aPL treatment caused a significant decrease in integrin alpha(1), alpha(5), and beta(1) proteins (86%, 84%, and 87%, respectively). We conclude that HTR-8/SVneo cell culture is a suitable model to study mechanisms of action of aPL on trophoblast, which in HTR-8/SVneo cells inhibit invasion by decreasing integrins alpha(5), alpha(1), and beta(1).
Jovanović
Milica
M
Institute for Application of Nuclear Energy - INEP, University of Belgrade, Serbia.
Bozić
Milica
M
Kovacević
Tamara
T
Radojcić
Ljiljana
L
Petronijević
Milos
M
Vićovac
Ljiljana
L
D017152:Antibodies, Antiphospholipid / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D039001:Integrin alpha Chains / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000166:cytology* / Q000187:drug effects*
D017152:Antibodies, Antiphospholipid; D039001:Integrin alpha Chains; D019012:Integrin beta1