Development of a high-performance liquid chromatographic method for the determination of a new potent radioiodinated melanoma imaging and therapeutic agent.
N-(2-diethylaminoethyl)-6-iodoquinoxaline-2-carbamide (ICF 01012) is a new melanoma imaging agent showing promising properties for application in internal radionuclide therapy. We developed an analytical protocol for detection of ICF 01012 in biological samples using HPLC. The proposed method was first validated using standard of ICF 01012 and four potent metabolites of this compound and then applied to follow the metabolic fate of [(125)I]ICF 01012 after injection in melanoma-bearing mice. The results demonstrate that this method exhibits a good linearity (r(2)=0.9947), specificity and acceptable accuracy. This simple method appears convenient and sufficient for pharmacokinetic studies on [(125)I]ICF 01012.
EA 4231 INSERM-Université d'Auvergne-Centre Jean Perrin, BP 184, 63005 Clermont-Ferrand cedex, France. Delphine.Denoyer@petermac.org
Delphine
D
Denoyer
Pierre
P
Labarre
Janine
J
Papon
Elisabeth
E
Miot-Noirault
Marie-Josephe
MJ
Galmier
Jean-Claude
JC
Madelmont
Jean-Michel
JM
Chezal
Nicole
N
Moins
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004195:Disease Models, Animal; D004355:Drug Stability; D007457:Iodine Radioisotopes / Q000378:metabolism; D016014:Linear Models; D008297:Male; D008546:Melanoma, Experimental / Q000000981:diagnostic imaging / Q000378:metabolism*; D051379:Mice; D009368:Neoplasm Transplantation; D011810:Quinoxalines / Q000378:metabolism*; D011877:Radionuclide Imaging; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D007457:Iodine Radioisotopes; C530303:N-(2-diethylaminoethyl)-6-iodoquinoxaline-2-carboxamide; D011810:Quinoxalines