[Study on extraction process of polysaccharide constituents from Panax japonicus by enzyme method].
OBJECTIVE
To choose the optimum extraction process of polysaccharide constituents from Panax japonicus.


METHODS
The optimal compound enzyme extraction process is selected with the orthogonal design. The yield of polysaccharide was used to evaluate the factor levels, so as to determine the condition of the optimum extraction process.


RESULTS
The conditions of the ideal extraction process using compound enzyme method were that of the pH value was at 5.0, the extraction temperature was at 50 degrees C, solvent volume was 50 times of material, cellulase quality score was 1.2%, pectinex quality score was 1.0% and the extraction time was 2 hours. At the last, the vitalitive enzyme was deactivated in the 90 degrees C water for 2 hours. Under these conditions, the yield of polysaccharide extract from P. japonicus was up to 14.3%.


CONCLUSIONS
The yield of polysaccharide extracted from P. japonicus can be effectively increased by using compound enzyme extraction process.
Department of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
Bin
B
Xu
Ping
P
Chen
Xin
X
Chen
D002480:Cellulase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D005894:Panax / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D011134:Polysaccharides; D002480:Cellulase