Transgenic breeding of anti-Bombyx mori L. nuclear polyhedrosis virus silkworm Bombyx mori.
Silkworm strains resistant to Bombyx mori L. nuclear polyhedrosis virus were obtained through transgenic experiments. piggyBac transposon with an A3 promoter were randomly inserted into the silkworm, driving the enhanced green fluorescent protein (EGFP) reporter gene into the silkworm genome. Polymerase chain reaction results verified the insertion of the extraneous EGFP gene, and fluorescence microscopy showed that the EGFP was expressed in the midgut tissue. The morbidity ratio of the nuclear polyhedrosis decreased from 90% in the original silkworm strain to 66.7% in the transgenic silkworm strain. Compared with the resistance to the Bombyx mori L. nuclear polyhedrosis virus in the Qiufeng strain, which is commonly used in the production, there was an increase of 33 centesimal points in the transgenic silkworms. The antivirotic character in the Chunhua x Qiuyue strain, which was bred from a different transgenic family, was about 10 centesimal points higher than that in the Qiufeng x Baiyu, another crossbreed used in production. Our results indicated a good application value of the transposon-inserted mutation in the breeding of anti-BmNPV silkworm strain.
College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.
Huijuan
H
Yang
Wei
W
Fan
Hao
H
Wei
Jinwei
J
Zhang
Zhonghua
Z
Zhou
Jianying
J
Li
Jianrong
J
Lin
Nong
N
Ding
Boxiong
B
Zhong
D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D016367:Baculoviridae / Q000235:genetics; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D001947:Breeding; D004251:DNA Transposable Elements; D017344:Genes, Insect; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic
D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins