Oxo-ester mediated native chemical ligation: concept and applications.
A direct oxo-ester peptide ligation method has been developed. Through the use of an activated C-terminal para nitrophenyl ester (1), it is possible to achieve direct cysteine ligations (1 + 2 --> 4). Peptide substrates incorporating bulky C-terminal amino acids (1) can be accommodated with high reaction efficiency.
Wan
Qian
Q
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA.
Chen
Jin
J
Yuan
Yu
Y
Danishefsky
Samuel J
SJ
D004921:Erythropoietin / Q000737:chemistry; D004952:Esters / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure
D004952:Esters; D004921:Erythropoietin