Synbiotic promotion of epithelial proliferation by orally ingested encapsulated Bifidobacterium breve and raffinose in the small intestine of rats.
We evaluated the effects of Bifidobacterium breve JCM1192(T )and/or raffinose on epithelial proliferation in the rat small and large intestines. WKAH/Hkm Slc rats (4 wk old) were fed a control diet, a diet supplemented with either encapsulated B. breve (30 g/kg diet, 1.5 x 10(7) colony-forming unit/g capsule) or raffinose (30 g/kg diet), or a diet supplemented with both encapsulated B. breve and raffinose, for 3 wk. Epithelial proliferation in the small intestine, as assessed by bromodeoxyuridine immunohistochemistry, was increased only in the B. breve plus raffinose-fed group. We determined the number of bifidobacteria in cecal contents using fluorescence in situ hybridization and confirmed the presence of ingested B. breve only in the B. breve plus raffinose-fed group. This suggests that the ingested B. breve cells used raffinose and were activated in the small intestine, where they subsequently influenced epithelial proliferation. In conclusion, we found a prominent synbiotic effect of encapsulated B. breve in combination with raffinose on epithelial proliferation in rat small intestine but not in large intestine. To our knowledge, this is the first report of a synbiotic that affects epithelial proliferation.
Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan. zuka@chem.agr.hokudai.ac.jp
Satoshi
S
Ishizuka
Ami
A
Iwama
Achmad
A
Dinoto
Akarat
A
Suksomcheep
Kohshi
K
Maeta
Takanori
T
Kasai
Hiroshi
H
Hara
Atsushi
A
Yokota
D000818:Animals; D001644:Bifidobacterium / Q000502:physiology*; D001973:Bromodeoxyuridine / Q000032:analysis; D002432:Cecum / Q000737:chemistry / Q000166:cytology / Q000382:microbiology; D002455:Cell Division / Q000187:drug effects*; D003106:Colon / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology*; D006863:Hydrogen-Ion Concentration; D007421:Intestine, Small / Q000166:cytology*; D008297:Male; D019936:Probiotics / Q000008:administration & dosage*; D011887:Raffinose / Q000008:administration & dosage*; D051381:Rats
D001973:Bromodeoxyuridine; D011887:Raffinose