[The role of Gilgamesh protein kinase in Drosophila melanogaster spermatogenesis].
The cellular function of the gilgamesh mutation (89B9-12) of casein kinase gene in Drosophila spermatogenesis was studied. It was demonstrated that the sterility resulting from this mutation is connected with the abnormalities in spermatid individualization. A phylogenetic study of the protein sequences of casein kinases 1 from various organisms was conducted. The Gilgamesh protein was shown to be phylogenetically closer to the cytoplasmic casein kinase family, represented by the YCK3, YCK2, and YCK1 proteins of Saccharomyces cerevisiae and animal gamma-casein kinases. It is known that these yeast casein kinases are involved in vesicular trafficking, which, in turn, is related in its genetic control to the cell membrane remodeling during spermatid individualization. Thus, the data of phylogenetic analysis fit well the results obtained by studying the mutation phenotype.
O O
OO
Nerusheva
N V
NV
Dorogova
N V
NV
Gubanova
L V
LV
Omel'ianchuk
D000818:Animals; D047389:Casein Kinase I / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D007248:Infertility, Male / Q000235:genetics / Q000378:metabolism; D008297:Male; D009154:Mutation; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013087:Spermatids / Q000378:metabolism* / Q000473:pathology; D013091:Spermatogenesis / Q000502:physiology*
D029721:Drosophila Proteins; D029701:Saccharomyces cerevisiae Proteins; D047389:Casein Kinase I; C479664:YCK1 protein, S cerevisiae; C479666:YCK2 protein, S cerevisiae; C479667:YCK3 protein, S cerevisiae; C479663:gish protein, Drosophila