Synthesis of 5- and 6-carboxy-X-rhodamines.
An efficient route is reported to 5- and 6-carboxy-X-rhodamines (compounds 1 and 2) that contain multiple n-propylene or gamma,gamma-dimethylpropylene groups bridging terminal nitrogen atoms and the central xanthene core. Gram quantities of these dyes are synthesized from inexpensive starting materials. The isolated products are activated by selective transformation of the carboxylic acid group into N-hydroxysuccinimidyl esters in situ and then conjugated with an amino group of a molecule of interest.
A. B. Hancock Jr. Memorial Laboratory for Cancer Research, Department of Biochemistry, Chemistry and Pharmacology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146, USA.
Md Jashim
MJ
Uddin
Lawrence J
LJ
Marnett
D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012235:Rhodamines / Q000138:chemical synthesis* / Q000737:chemistry
C533378:5-carboxy-X-rhodamine; C518402:6-carboxy-X-rhodamine; D005456:Fluorescent Dyes; D012235:Rhodamines