Estradiol induces sexual behavior in female túngara frogs.
Steroid hormones play an important role in regulating vertebrate sexual behavior. In frogs and toads, injections of exogenous gonadotropins, which stimulate steroid hormone production, are often used to induce reproductive behavior, but steroid hormones alone are not always sufficient. To determine which hormonal conditions promote sexual behavior in female túngara frogs, we assessed the effect of hormone manipulation on the probability of phonotaxis behavior toward conspecific calls in post-reproductive females. We injected females with human chorionic gonadotropin (HCG), estradiol, estradiol plus progesterone, saline, or HCG plus fadrozole (an aromatase blocker) and tested their responses to mating calls. We found that injections of HCG, estradiol, and estradiol plus progesterone all increased phonotaxis behavior, whereas injections of saline or HCG plus fadrozole did not. Since injections of estradiol alone were effective at increasing phonotaxis behavior, we concluded that estradiol is sufficient for the expression of phonotaxis behavior. Next, to determine if estradiol-injected females display the same behavioral preferences as naturally breeding females, we compared mating call preferences of naturally breeding females to those of post-reproductive females injected with estradiol. We found that, when injected with estradiol, females show similar call preferences as naturally breeding females, although they were less likely to respond across multiple phonotaxis tests. Overall, our results suggest that estradiol is sufficient for the expression of sexual responses to mating calls in túngara frogs. To our knowledge, ours is the only study to find that estradiol alone is capable of promoting phonotaxis behavior in a frog.
Chakraborty
Mukta
M
Department of Biology, University of North Carolina, Chapel Hill, NC 27599-3280, USA. mukta@email.unc.edu
Burmeister
Sabrina S
SS
D000161:Acoustic Stimulation; D000704:Analysis of Variance; D000818:Animals; D001001:Anura / Q000502:physiology*; D047072:Aromatase Inhibitors / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000097:blood / Q000494:pharmacology*; D017316:Fadrozole / Q000494:pharmacology; D005260:Female; D006801:Humans; D011374:Progesterone / Q000097:blood / Q000494:pharmacology; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D012919:Social Behavior; D014828:Vocalization, Animal
D047072:Aromatase Inhibitors; D006063:Chorionic Gonadotropin; D011374:Progesterone; D004958:Estradiol; D017316:Fadrozole