Molecular typing and characterization of extended-spectrum TEM, SHV and CTX-M beta-lactamases in clinical isolates of Enterobacter cloacae.
Sixty-one non-repetitive Enterobacter cloacae ESBL producers were collected at the Amiens University Hospital in France. Eight beta-lactam resistance phenotypes (a-h) and three aminoglycoside resistance phenotypes (i-k) were identified among these isolates, and 32 different pulsotypes were observed. Of these 61 isolates, 37 were sequenced and found to harbor beta-lactamases with a pI of 5.9 (TEM-4), 6.5 (TEM-24), 7.8 (SHV-4), 8.2 (SHV-12), 8.4 (CTX-M-1) and 8.0 (CTX-M-9). Four imipenem-resistant ESBL-producing E. cloacae isolates did not express the 38kDa OMP, indicating that this resistance is associated with porin deficiency.
Service de Bactériologie, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, France. Biendo.Maurice@chu-amiens.fr
Maurice
M
Biendo
Claudia
C
Manoliu
Geneviève
G
Laurans
Sandrine
S
Castelain
Brigitte
B
Canarelli
Danièle
D
Thomas
Farida
F
Hamdad
Florence
F
Rousseau
François
F
Eb
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D016972:Enterobacter cloacae / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D018272:Porins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D055815:Young Adult; D001618:beta-Lactamases / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D018272:Porins; D015378:Imipenem; D001618:beta-Lactamases