Validation of volume-pressure recording tail-cuff blood pressure measurements.
BACKGROUND
The American Heart Association has recommended tail-cuff blood pressure measurement for high-throughput experimental designs, including mutagenesis screens and genetic crosses. However, some tail-cuff methods show good agreement with radiotelemetry and others do not, indicating that each tail-cuff method requires independent validation.


METHODS
We validated the volume-pressure recording (VPR) tail-cuff method by comparison to simultaneous radiotelemetry measurements.


RESULTS
Bland-Altman analysis of 560 cycles from 26 independent measurement sessions showed good agreement between VPR and radiotelemetry measurements, with tail-cuff measurements being 0.25 mm Hg lower than telemetry measurements on average. However, the VPR method was less accurate, compared to radiotelemetry, at extreme high and low (i.e., <110 or >180 mm Hg) systolic blood pressures (SBPs).


CONCLUSIONS
We conclude that the VPR tail-cuff method provides accurate blood pressure measurements over the physiological range of blood pressure in mice.
Novartis Institutes for BioMedical Research & Novartis Pharmaceutical Corporation, East Hanover, NJ, USA.
Minjie
M
Feng
Steven
S
Whitesall
Yunyu
Y
Zhang
Martin
M
Beibel
Louis
L
D'Alecy
Keith
K
DiPetrillo
D000818:Animals; D001794:Blood Pressure; D001795:Blood Pressure Determination / Q000295:instrumentation* / Q000379:methods*; D015924:Blood Pressure Monitors; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D013623:Tail / Q000098:blood supply*; D013686:Telemetry / Q000295:instrumentation / Q000379:methods