Expression profile analysis of aorta-gonad-mesonephros region-derived stromal cells reveals genes that regulate hematopoiesis.
The aorta-gonad-mesonephros (AGM) region is involved in the generation and maintenance of the first definitive hematopoietic stem cells (HSCs). A mouse AGM-derived cell line, AGM-S3, was shown to support the development of HSCs. To elucidate the molecular mechanisms regulating early hematopoiesis, we obtained subclones from AGM-S3, one of which was hematopoiesis supportive (S3-A9) and the other one of which was non-supportive (S3-A7), and we analyzed their gene expression profiles by gene chip analysis. In the present study, we found that Glypican-1 (GPC1) was highly expressed in the supportive subclone AGM-S3-A9. Over-expression of GPC1 in non-supportive cells led to the proliferation of progenitor cells in human cord blood when cocultured with the transfected-stromal cells. Thus, GPC1 may have an important role in the establishment of a microenvironment that supports early events in hematopoiesis.
Nagao
Kenji
K
Frontier Laboratory, Kirin Pharma CO, LTD, Takasaki, Gunma, Japan.
Ohta
Takayuki
T
Hinohara
Atsushi
A
Tahara
Tomoyuki
T
Hagiwara
Tetsuya
T
Maeda
Yoshitake
Y
Yoneya
Takashi
T
Sohma
Yoshiaki
Y
Heike
Toshio
T
Nakahata
Tatsutoshi
T
Inagaki
Yoshimasa
Y
Nishikawa
Mitsuo
M
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053673:Glypicans / Q000235:genetics* / Q000502:physiology; D006066:Gonads / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D006410:Hematopoiesis / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008650:Mesonephros / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014162:Transfection
D053673:Glypicans