Phycicoccus aerophilus sp. nov., isolated from air.
A Gram-positive, short rod-shaped, non-spore-forming bacterium, designated 5516T-20(T), was isolated from an air sample. The results of 16S rRNA gene sequence analyses showed that strain 5516T-20(T) belonged to the family Intrasporangiaceae, having the highest sequence similarities (97.5 and 96.1 %, respectively) with respect to the type strains of Phycicoccus dokdonensis and Phycicoccus jejuensis. The value for DNA-DNA hybridization between 5516T-20(T) and P. dokdonensis DS-8(T) was 41 %. Strain 5516T-20(T) contained menaquinone MK-8(H4) as the major isoprenoid quinone, possessed phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol as the polar lipids and contained glucose and ribose as whole-cell sugars. The major fatty acids were C(17 : 1)omega8c, iso-C(16 : 0), iso-C(15 : 0), C(17 : 0) and iso-C(14 : 0). The G+C content of the DNA from strain 5516T-20(T) was 70.5 mol%. On the basis of the data from the polyphasic taxonomic study, strain 5516T-20(T) represents a novel species within the genus Phycicoccus, for which the name Phycicoccus aerophilus sp. nov. is proposed. The type strain is 5516T-20(T) (=KACC 20658(T) =DSM 18548(T)).
Weon
Hang-Yeon
HY
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Yoo
Seung-Hee
SH
Kim
Byung-Yong
BY
Schumann
Peter
P
Kroppenstedt
Reiner M
RM
Hong
Sung-Kee
SK
Kwon
Soon-Wo
SW
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000391:Air Microbiology; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8