Amendment with peony root bark improves the biocontrol efficacy of Trichoderma harzianum against Rhizoctonia solani.
We tested Trichoderma harzianum as a biocontrol agent for Rhizoctonia solani AG2-1, using six natural antifungal materials to improve its efficacy. Among the six materials tested, peony (Paeonia suffruticosa) root bark (PRB) showed the strongest antifungal activity against R. solani AG2-1, and was not antagonistic to T. harzianum. Scanning electron microscopy showed that treatment with PRB extract resulted in shortened and deformed R. solani AG2-1 hyphal cells. The control of radish damping-off caused by R. solani AG2-1 was greatly increased by combined treatments of T. harzianum and PRB, as compared with either of the two treatments alone, with the control effect increased from 42.3-51.5% to 71.4-87.6%. The antifungal compound in PRB, which was isolated in chloroform and identified as paeonol by mass spectrometry, 1H NMR, and 13C NMR analyses, inhibited the growth of R. solani AG2-1 but not that of T. harzianum. Thus, PRB powder or extract may be used as a safe additive to T. harzianum to improve the control of the soil borne diseases caused by R. solani AG2-1.
Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-921, Korea.
Tae Ok
TO
Lee
Zakaullah
Z
Khan
Sang Gyu
SG
Kim
Young Ho
YH
Kim
D000704:Analysis of Variance; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D019906:Nuclear Magnetic Resonance, Biomolecular; D029596:Paeonia / Q000737:chemistry; D010572:Pest Control, Biological / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D031224:Raphanus / Q000382:microbiology; D012232:Rhizoctonia / Q000187:drug effects* / Q000254:growth & development* / Q000472:pathogenicity; D012988:Soil Microbiology; D014242:Trichoderma / Q000187:drug effects / Q000502:physiology*
D000935:Antifungal Agents; D010936:Plant Extracts