Blockade of alpha6-integrin reveals diversity in homing patterns among human, baboon, and murine cells.
Our understanding of the mechanisms by which intravenously transplanted hematopoietic stem/progenitor cells (HSPCs) home to and engraft the bone marrow (BM) remains incomplete, but participation of adhesion molecules has been documented. We here demonstrate that blockade of the alpha6-integrin enhanced BM homing of human and nonhuman primate BM-derived HSPCs by >60% in the xenogeneic transplant model and led to significantly improved engraftment. The effect was limited to BM-derived HSPCs, as granulocyte-colony-stimulating factor mobilized peripheral blood or cord blood HSPCs express little or no alpha6 integrin. By contrast, despite high alpha6 integrin expression, no effect of alpha6 blockade on murine BM-HSPCs homing/engraftment was observed.
Bonig
Halvard
H
Department of Medicine Division of Hematology, University of Washington, Seattle, Washington, USA.
Priestley
Gregory V
GV
Wohlfahrt
Martin
M
Kiem
Hans-Peter
HP
Papayannopoulou
Thalia
T
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D002465:Cell Movement; D003114:Colony-Forming Units Assay; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D039503:Integrin alpha6 / Q000378:metabolism*; D051379:Mice; D010215:Papio / Q000276:immunology*
D018952:Antigens, CD34; D039503:Integrin alpha6