A non-selective cationic channel activated by diacylglycerol in mouse intestinal myocytes.
Application of 1-oleoyl-2-acetyl-sn-glycerol (OAG), an analogue of diacylglycerol (DAG) formed via M(3) muscarinic receptors, induced inward cationic currents via a protein kinase C-independent mechanism and produced membrane depolarization with increased action potential discharges in mouse intestinal myocytes. Outside-out patches from the myocytes responded to OAG with openings of 115-pS channels characterized by a mean open time (O(tau)) of 0.15 ms. M(3) receptor stimulation is reportedly capable of causing brief openings (O(tau)=0.23 ms) of 120-pS cationic channels in intestinal myocytes, thus the present results strongly support the idea that the M(3)-mediated 120-pS channel opening is brought about via DAG-dependent mechanisms.
Department of Pathogenetic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Takashi
T
Sakamoto
Hayato
H
Matsuyama
Masayuki
M
Yamamoto
Yasuyuki
Y
Tanahashi
Takio
T
Kitazawa
Tetsuro
T
Taneike
Seiichi
S
Komori
Toshihiro
T
Unno
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002412:Cations / Q000378:metabolism; D004075:Diglycerides / Q000494:pharmacology*; D004594:Electrophysiology; D005260:Female; D007421:Intestine, Small / Q000378:metabolism; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D011493:Protein Kinase C / Q000378:metabolism; D043587:Receptor, Muscarinic M3 / Q000378:metabolism*
D002412:Cations; D004075:Diglycerides; D007473:Ion Channels; D043587:Receptor, Muscarinic M3; C038237:1-oleoyl-2-acetylglycerol; D011493:Protein Kinase C