Oxidative stress intensity in lens and aqueous depending on age-related cataract type and brunescense.
OBJECTIVE
Cataract formation represents a serious problem in the elderly, and has a large impact on healthcare budget. The oxidative stress form and intensity might determine the cataract type and pigmentation, making efforts in the cataract prevention challenge more complex.


METHODS
This is a retrospective cross-sectional review of 80 samples of aqueous humor and lens corticonuclear blocks. Aqueous samples were analyzed by the method of antioxidant activity estimation (%iMDA), while lipid peroxides (LP) and total sulfhydryl groups (TSH) were determined in lenses.


RESULTS
Mixed and brunescent cataracts have statistically significant lower values of antioxidative %iMDA and TSH (p<0.001 for both parameters) and higher values of lipid peroxidation (p<0.001). No correlation between LP and TSH with maturity of cortical cataract was found, but there was a significant correlation with the %iMDA (p<0.05).


CONCLUSIONS
The role of the oxidative stress in cataractogenesis could not be the same for all cataract types. High level of lipid peroxides in pigmented cataracts may point to the different nature of pigment source than proteins solely, whereas lipid peroxidation and SH groups consumption in cortical cataractogenesis might be of less importance.
Zoric
L
L
Clinic for Eye Diseases, Faculty of Medicine, Settlement Kosovska Mitrovica - Serbia. lepsaz@eunet.yu
Elek-Vlajic
S
S
Jovanovic
M
M
Kisic
B
B
Djokic
O
O
Canadanovic
V
V
Cosic
V
V
Jaksic
V
V
D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism; D001082:Aqueous Humor / Q000378:metabolism*; D002386:Cataract / Q000145:classification / Q000378:metabolism*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008875:Middle Aged; D018384:Oxidative Stress; D012189:Retrospective Studies; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D000975:Antioxidants; D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D008315:Malondialdehyde