An efficient fluorimetric method to measure the viability of intraerythrocytic Plasmodium falciparum.
A range of various assays to measure chemosusceptibility of Plasmodium falciparum have been described in the literature. As the screening of a plethora of compounds for antiplasmodial activity is urgently needed and becomes a constantly increasing routine analysis, a test system has to fulfill the following requirements: sensitivity, reliability, simplicity of performance, high-throughput compatibility, and cost-effectiveness. Here, we describe an assay that fulfills all criteria and in which the fluorescent SYTOX Green dye is introduced to determine growth inhibition of Plasmodia in in vitro cultures.
Evers
Astrid
A
1Department of Zoophysiology, Zoological Institute, University of Kiel, Olshausenstr. 40, D-24098 Kiel, Germany.
Heppner
Saskia
S
Leippe
Matthias
M
Gelhaus
Christoph
C
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002738:Chloroquine / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000191:economics* / Q000379:methods*; D004912:Erythrocytes / Q000469:parasitology*; D005456:Fluorescent Dyes; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009930:Organic Chemicals; D010963:Plasmodium falciparum / Q000187:drug effects / Q000502:physiology*; D013050:Spectrometry, Fluorescence
D000962:Antimalarials; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C402795:SYTOX Green; D002738:Chloroquine; D007770:L-Lactate Dehydrogenase