Expression of cystic fibrosis transmembrane conductance regulator in rat ovary.
The protein expression of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated Cl(-) channel, in ovarian stimulated premature female rat ovary during a cycle of follicle development and corpus luteum formation was investigated. Animals were injected with 10 U pregnant Mare's serum gonadotropin (PMSG) and subsequently 10 U hCG 48 h later. Time-dependent immunohistochemistry and Western blotting experiments were performed before and 24, 48, 72 h after hCG treatment. The immunohistochemistry revealed that administration of PMSG stimulated the CFTR expression in thecal cell layer and granulosa cell layer of mature follicles 48 h post injection, coincident with the PMSG-induced peak in follicular estradiol. However, the expression of CFTR in the granulose lutein cell layer and thecal lutein cell layer was time-dependently reduced following hCG injection, in accordance with the gradually increased progestogen level during luteum corpus formation. Western blotting analysis demonstrated that rat ovarian tissue expressed the special CFTR band at 170 kD. It is concluded that cAMP-dependent Cl(-) channels are involved in regulation of follicle development and luteum formation.
Reproductive Medical Center, Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. ljin911@126.com
Lei
L
Jin
Ruiling
R
Tang
D000818:Animals; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D003338:Corpus Luteum / Q000254:growth & development; D005260:Female; D006080:Ovarian Follicle / Q000254:growth & development; D010053:Ovary / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
C526316:CCN2 protein, rat; D055513:Connective Tissue Growth Factor