[Analysis of matrine and oxymatrine in Kushensu injection by UPLC/Q-TOF-MS].
OBJECTIVE
To develop a method for the determination of matrine and oxymatrine in Kushensu injection by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS).


METHODS
The analysis was performed on a Acquity UPLC BEH C18 column (2.1 mm x 100 mm, 1.7 microm) with the gradient elution of 10 mmol x L(-1) amine acetate (pH 8, adjusted by aqueous ammonia) and methanol. The flow rate was set as 0.30 mL x min(-1). The column temperature was maintained at 30 degrees C. The peaks were detected at 210 nm and the injection volume was 2 microL.


RESULTS
The calibration curves showed good linearity within the test ranges of 0.084-3.36 microg for matrine and 0.086-3.44 microg for oxymatrine, respectively. The mean recoveries were 103.2%, 98.7%, and the RSD were 1.5%, 1.2%, respectively.


CONCLUSIONS
The established method is simple, rapid and sensitive, can be used for the quality control of matrine and oxymatrine in Kushensu injection through the manufacturing process and clinical implement.
Ye
Mao
M
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Xie
Guo-xiang
GX
Zhao
Ai-hua
AH
Zhang
Yuan-yuan
YY
Jia
Wei
W
D000470:Alkaloids / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry*; D007267:Injections; D016014:Linear Models; D011807:Quinolizines / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors; D000093842:Matrines
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; C540578:kushensu; C037573:oxymatrine; D000093842:Matrines