In vivo verification of protein interactions in the inner ear by coimmunoprecipitation.
Genomics has provided us with vast amounts of data and thus, the challenge to identify and characterize gene products. Proteomics analysis, using methods such as yeast two-hybrid screenings, isoelectric focusing, and mass spectroscopy, generate potentially useful information. To determine functional relationships between and among proteins, however, the initial data for putative protein interactions must first be validated. One technique, which is considered the gold standard, is coimmunoprecipitation.
Harvey
Margaret C
MC
Department of Otolaryngology-HNS, University of South Florida, College of Medicine, Tampa, FL, USA.
Sokolowski
Bernd H A
BH
D000818:Animals; D015153:Blotting, Western; D007758:Ear, Inner / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D047468:Immunoprecipitation / Q000379:methods*; D011485:Protein Binding