Aptabodies - new type of artificial receptors for detection proteins.
We report on a new type of artificial receptor formed by hybridization of two DNA aptamers for human thrombin (aptabody). This aptasensor based on multiwalled carbon nanotubes allowed us to detect thrombin with detection limit of 0.3 nM, which was 3 times better in comparison with conventional aptamer.
Department of Nuclear Physics and Biophysics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, Slovakia. tibor.hianik@fmph.uniba.sk
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Hianik
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Porfireva
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Grman
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Evtugyn
D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D019921:Biotinylation; D004563:Electrochemistry; D006801:Humans; D008751:Methylene Blue / Q000378:metabolism; D037742:Nanotubes, Carbon; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D010196:Pancreatic Elastase / Q000378:metabolism; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism*
D052157:Aptamers, Nucleotide; D037742:Nanotubes, Carbon; D012709:Serum Albumin; D010196:Pancreatic Elastase; D013917:Thrombin; D008751:Methylene Blue