Thin frozen film method for visualization of storage proteins in mature rice grains.
There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.
Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.
Yuhi
Y
Saito
Nobuaki
N
Nakatsuka
Takanari
T
Shigemitsu
Kunisuke
K
Tanaka
Shigeto
S
Morita
Shigeru
S
Satoh
Takehiro
T
Masumura
D005629:Frozen Sections / Q000379:methods*; D012275:Oryza / Q000254:growth & development; D010940:Plant Proteins
D010940:Plant Proteins