An integrative approach for analyzing the interplay of genetic and epigenetic changes in tumors.
The accumulation of chromosomal aberrations is a characteristic feature of tumor development. However, an understanding of tumorigenesis that assumes that changes in DNA copy number always cause equivalent changes in the corresponding RNA and protein levels is an oversimplification and completely ignores the individual genetic and epigenetic context in which an aberration has to be evaluated. We present a brief introduction to various techniques dedicated to the genome-wide analysis of genetic and epigenetic changes, and illustrate how complementary information derived from these various DNA array-based technologies can lead to a better understanding of the consequences of chromosomal aberrations.
Muradyan
Artur
A
Department of Human Molecular Genetics, Max Planck Institute for Molecular Genetics, Berlin, Germany.
Boldt
Vivien
V
Steininger
Anne
A
Stabentheiner
Stephanie
S
Tebel
Katrin
K
Kreutzberger
Jürgen
J
Müller
Ines
I
Madle
Hannelore
H
Popper
Helmut H
HH
Ullmann
Reinhard
R
D002869:Chromosome Aberrations; D044127:Epigenesis, Genetic / Q000235:genetics*; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis