IgA response by oral infection with an attenuated Yersinia enterocolitica mutant: implications for its use as oral carrier vaccine.
Yersinia enterocolitica (Ye) mutant strain (sycH-) is unable to secrete the virulence protein YopH. Mucosal vaccination is often required to induce protection, but stimulating strong IgA response is frequently difficult. Here, we addressed whether Ye sycH- might induce IgA response, and investigated its attenuation in TNFRp55-/-, IL-12p40-/- and IL-4-/- mice. We found that Ye sycH- colonizes Peyer's patches, and induces higher Yersinia-specific IgA levels in feces and in serum compared with Ye wild type. The Ye sycH-mutant proved to be attenuated and induced IgA in both wild-type and immunodeficient mice. These lines of evidence show the attenuation of Ye sycH- and its ability to stimulate an IgA response. This mutant might be useful as an oral vaccine carrier.
Immunology, Laboratory of Microbiology, Chemistry, Biochemistry and Pharmacy Faculty, National University of San Luis, IMIBIO-SL, CONICET, San Luis, Argentina.
Helga M
HM
Blanco
María G
MG
Lacoste
Ricardo J
RJ
Eliçabe
María S
MS
Di Genaro
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D001428:Bacterial Vaccines; D005243:Feces / Q000382:microbiology; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000097:blood* / Q000276:immunology; D015847:Interleukin-4 / Q000172:deficiency; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation; D010581:Peyer's Patches / Q000276:immunology / Q000382:microbiology; D015009:Yersinia Infections / Q000276:immunology*; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D015847:Interleukin-4