Entamoeba histolytica: response of the parasite to metronidazole challenge on the levels of mRNA and protein expression.
Entamoeba histolytica remains a cause of significant morbidity and mortality in many countries. Although metronidazole has been used for the treatment of amoebiasis for several decades, little is known on how the amoebae react to this challenge on the levels of mRNA and protein expression. In this study, we examined their response using a focused microarray, quantitative RT-PCR, and two-dimensional gel electrophoresis (2DE). The amoebae modestly increased the levels of mRNA coding for superoxide dismutase, peroxiredoxin, ferredoxin, thioredoxin reductase, and the galactose/N-acetylgalactosamine specific lectin light and heavy chains. The mRNAs encoding actin and the 70kDa and 101kDa heat shock proteins were decreased. All the changes occured within 1h of exposition, with very little further changes. In addition, the proteome revealed only very few changes. Taken together, E. histolytica appears to make only modest mRNA and protein expression changes when confronted with an unknown chemical stress.
Department of Specific Prophylaxis and Tropical Medicine, Center for Physiology, Pathophysiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria.
Margit
M
Tazreiter
David
D
Leitsch
Evelyn
E
Hatzenbichler
Georg E
GE
Mair-Scorpio
Ralf
R
Steinborn
Martin
M
Schreiber
Michael
M
Duchêne
D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004748:Entamoeba histolytica / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D008795:Metronidazole / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome / Q000096:biosynthesis / Q000235:genetics; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics; D013880:Thioredoxin-Disulfide Reductase / Q000096:biosynthesis / Q000235:genetics; D013879:Thioredoxins / Q000096:biosynthesis / Q000235:genetics
D000199:Actins; D000981:Antiprotozoal Agents; D018840:HSP70 Heat-Shock Proteins; D020543:Proteome; D015800:Protozoan Proteins; D012333:RNA, Messenger; D008795:Metronidazole; D013879:Thioredoxins; D013482:Superoxide Dismutase; D013880:Thioredoxin-Disulfide Reductase