Chronic morphine application is protective against cell death in primary human neurons.
Morphine addiction has become a long-time, serious medical and social problem. To understand the cellular mechanisms of morphine application and addiction, the effects of chronic morphine treatments were examined in primary cultured human neurons. Our results show that, surprisingly, morphine protects cultured human neurons against serum deprivation and staurosporine induced cytotoxicity. Morphine downregulates proapoptotic factor Bax levels in cultured human neurons. In addition, heat shock protein 70 is also involved in morphine protection. Our data suggest that chronic morphine application may be beneficial to stimulate cell survival.
Cui
Jia
J
Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing, China.
Chen
Qiuyue
Q
Yu
Long-Chuan
LC
Zhang
Yan
Y
D000701:Analgesics, Opioid / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D009020:Morphine / Q000494:pharmacology*; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D019311:Staurosporine / Q000494:pharmacology; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D000701:Analgesics, Opioid; D004791:Enzyme Inhibitors; D018840:HSP70 Heat-Shock Proteins; D009292:Narcotic Antagonists; D051028:bcl-2-Associated X Protein; D009270:Naloxone; D009020:Morphine; D019311:Staurosporine