Proteomic analysis of the hyaloid vascular system regression during ocular development.
We describe a proteomic approach to investigate the differential protein expression patterns and identify the physiologically relevant angiogenic and antiangiogenic factors involved in the hyaloid vascular system regression. Differentially expressed proteins were identified using two-dimensional gel electrophoresis followed by nanoflow chromatography coupled with tandem mass spectrometry. These proteins are expected to provide insight as to their function in the early maintenance and eventual regression of the hyaloid vascular system.
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
Elena
E
Albè
Jin-Hong
JH
Chang
Nathalie F
NF
Azar
Alexander R
AR
Ivanov
Dimitri T
DT
Azar
D000818:Animals; D000831:Animals, Newborn; D005123:Eye / Q000098:blood supply* / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009799:Ocular Physiological Phenomena; D038081:Organogenesis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods
D020543:Proteome