Quantitative genotyping by amplifying the polymorphic sequences of Pre-Melanosomal Protein (PMEL17) gene using real-time polymerase chain reaction in chickens.
1. This study was conducted to develop a quantitative genotyping system of chimaeric chickens by real-time PCR. 2. The polymorphisms in exons 7 and 11 of PMEL17 gene, which is one of the major genes affecting plumage colour, were identified from White Leghorn, Barred Plymouth Rock and Rhode Island Red chickens. 3. Quantitative genotyping was successfully performed by real-time PCR using polymorphic sequence-specific TaqMan Probes. 4. This methodology can support future research of germline chimaeric chickens as well as the application of germ cell transfer technique.
Transgenic Animal Research Center, Tsukuba, Ibaraki 305-8602, Japan.
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Minematsu
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Harumi
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Naito
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D005838:Genotype; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D011110:Polymorphism, Genetic; D058951:gp100 Melanoma Antigen
D008562:Membrane Glycoproteins; D058951:gp100 Melanoma Antigen