Identification of a novel polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) gene required for the biosynthesis of cyclopiazonic acid in Aspergillus oryzae.
Cyclopiazonic acid (CPA) is a mycotoxin produced by several strains of Penicillium and Aspergillus species. Aspergillus oryzae strains used in fermented foods do not produce CPA; however, several wild-type A. oryzae strains produce CPA. Here, we identified a novel polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) gene involved in CPA production by comparing the telomere-adjacent region of a CPA-producing strain (A. oryzae NBRC 4177) with that of a nonproducing strain (A. oryzae RIB40). NBRC 4177 has an additional 17-18-kb sequence beyond the region corresponding to the telomere repeat in RIB40 and this additional regions contains 3' region of the PKS-NRPS gene, while RIB40 has only the 5' region of the PKS-NRPS gene. Gene disruption of the PKS-NRPS gene in NBRC 4177 resulted in elimination of CPA production. Thus, the PKS-NRPS gene is required for CPA biosynthesis, and the truncation of this gene is presumed as one of the determinants of CPA nonproductivity in A. oryzae RIB40.
Tokuoka
Masafumi
M
Noda Institute for Scientific Research, 399 Noda, Noda, Chiba 278-0037, Japan. tokuoka@nrib.go.jp
Seshime
Yasuyo
Y
Fujii
Isao
I
Kitamoto
Katsuhiko
K
Takahashi
Tadashi
T
Koyama
Yasuji
Y
D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics; D017353:Gene Deletion; D016681:Genome, Fungal; D007211:Indoles / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D010453:Peptide Synthases / Q000235:genetics* / Q000378:metabolism*; D048630:Polyketide Synthases / Q000235:genetics* / Q000378:metabolism*; D016615:Telomere
D007211:Indoles; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase; C000543:cyclopiazonic acid