Semicarbazide-sensitive amine oxidase activity and total nitrite and nitrate concentrations in serum: novel biochemical markers for type 2 diabetes?
The aim of this study was to evaluate the activity of semicarbazide-sensitive amine oxidase (SSAO) and the total nitrite and nitrate (NO( x )) concentrations in serum from type 2 diabetic patients and control subjects in order to evaluate if they could be used as novel diabetic markers. We studied 38 type 2 diabetic patients and 35 control subjects. Serum samples from those subjects were evaluated by radiochemical methods for SSAO activity using (14)C-benzylamine. Serum NO( x ) concentrations were obtained as an index of nitric oxide production by the Griess reaction. Serum SSAO activity was higher in type 2 diabetic patients than in control group and serum SSAO in type 2 diabetic correlated with age, serum creatinine and total cholesterol. Serum NO( x ) levels in type 2 diabetic patients were also significantly higher than those in the control group. Serum NO( x ) levels in control group correlated with serum SSAO activity. In conclusion, the increase in the SSAO activity and NO( x ) levels observed in type 2 diabetic patients could be parameters to take in account and play relevant role in diabetes development. SSAO and NO( x ) are suggested as markers for prognostic of diabetes.
Laboratory of Pharmacology, Faculty of Pharmacy, University of Coimbra, 3000-175, Coimbra, Portugal.
Sandra Fernanda
SF
Nunes
Isabel Vitoria
IV
Figueiredo
Paulo João
PJ
Soares
Nuria Espriu
NE
Costa
Maria Celeste
MC
Lopes
Maria Margarida
MM
Caramona
D000284:Administration, Oral; D000368:Aged; D000369:Aged, 80 and over; D000419:Albuminuria / Q000453:epidemiology; D006631:Amine Oxidase (Copper-Containing) / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000188:drug therapy / Q000201:enzymology; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000097:blood*; D009569:Nitric Oxide / Q000097:blood*; D009573:Nitrites / Q000097:blood*; D011379:Prognosis; D012016:Reference Values; D015203:Reproducibility of Results; D014280:Triglycerides / Q000097:blood
D006631:Amine Oxidase (Copper-Containing); D015415:Biomarkers; D001786:Blood Glucose; D002784:Cholesterol; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D009566:Nitrates; D009569:Nitric Oxide; D009573:Nitrites; D014280:Triglycerides