Real-time PCR analysis for blood cell lineage specific markers.
We here describe the methods for the isolation of distinct hematopoietic subpopulations, as defined by their immune phenotype by fluorescence-activated cell sorting, and how these cells can be analyzed even at a single-cell level for the gene expression of a number of transcription factors and other differentiation markers.
Edvardsson
Louise
L
Division of Hematology and Transfusion Medicine, Department of Laboratory Medicine, Biomedical Center, Lund University, Lund, Sweden.
Olofsson
Tor
T
D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000235:genetics; D001773:Blood Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D005434:Flow Cytometry / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000502:physiology*; D006410:Hematopoiesis / Q000502:physiology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D000943:Antigens, Differentiation