Lymphatic delivery of 99mTc-labeled dextran acetate particles including cyclosporine A.
Biodistribution and lymphoscintigraphy of cyclosporine A (CyA) and technetium-99m (99mTc) were studied using 99mTc-labeled dextran acetate (DxA) including CyA. DxA particles were prepared from dextran with acetic anhydride, and CyA was loaded into them. Lymphatic delivery of 99mTc-labeled DxA particles containing CyA was evaluated after subcutaneous injection into the foot pad of rats and compared with those of 99mTc-labeled human serum albumin (HSA). The labeling efficiency of CyA-loaded 99mTc-DxA particles was about 95% at 30 min. The labeling efficiency maintained stably above 80% for 12 h. The percent injected dose (%ID) of CyA-loaded 99mTc-DxA was similar to that of 99mTc-HSA at the inguinal lymph node after 40 min. The CyA-loaded 99mTc-DxA could be as well distributed as 99mTc-HSA through the lymph node. The DxA particles could steadily distribute the CyA as well as the 99mTc radiolabeling through the lymph node.
Kim
Jin
J
Department of Advanced Chemicals Graduate School, Chonnam National University, Gwangju 500-757, Korea.
Chung
Kyong-Hwan
KH
Lee
Chang-Moon
CM
Seo
Young-Soon
YS
Song
Ho-Chun
HC
Lee
Ki-Young
KY
D000818:Animals; D016572:Cyclosporine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D003911:Dextrans / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems / Q000379:methods; D004355:Drug Stability; D005260:Female; D042601:Lymphatic Vessels / Q000000981:diagnostic imaging / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D011877:Radionuclide Imaging / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000008:administration & dosage / Q000493:pharmacokinetics; D017550:Spectroscopy, Fourier Transform Infrared; D013667:Technetium / Q000493:pharmacokinetics*; D013668:Technetium Tc 99m Aggregated Albumin / Q000493:pharmacokinetics; D014018:Tissue Distribution
D003911:Dextrans; D012709:Serum Albumin; D013668:Technetium Tc 99m Aggregated Albumin; D013667:Technetium; D016572:Cyclosporine