Proteomic analysis of protein S-nitrosylation.
Nitric oxide (NO) produces covalent PTMs of specific cysteine residues, a process known as S-nitrosylation. This route is dynamically regulated and is one of the major NO signalling pathways known to have strong and dynamic interactions with redox signalling. In agreement with this scenario, binding of NO to key cysteine groups can be linked to a broad range of physiological and pathological cellular events, such as smooth muscle relaxation, neurotransmission and neurodegeneration. The characterization of S-nitrosylated residues and the functional relevance of this protein modification are both essential information needed to understand the action of NO in living organisms. In this review, we focus on recent advances in this field and on state-of-the-art proteomic approaches which are aimed at characterizing the S-nitrosylome in different biological backgrounds.
Mass Spectrometry Unit DIBIT, San Raffaele Scientific Institute, Milano, Italy.
Federico
F
Torta
Vera
V
Usuelli
Antonio
A
Malgaroli
Angela
A
Bachi
D000818:Animals; D001710:Biotin / Q000737:chemistry; D003545:Cysteine / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D013058:Mass Spectrometry; D009569:Nitric Oxide / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
D011506:Proteins; D009569:Nitric Oxide; D001710:Biotin; D003545:Cysteine