[Predictive and prognostic markers of breast cancer. Molecular biological analysis of fixed tumor tissue].
A multitude of prognostic and predictive multiparameter algorithms based on the analysis of mRNA have been published in recent years. Many of the algorithms require fresh or fresh frozen tissue as a source of the mRNA. However, practical considerations suggest formalin-fixed paraffin-embedded tissue (FFPE tissue) to be a more suitable starting material for routine diagnostic applications. Therefore, Siemens Healthcare Diagnostics is developing a fully automated method to extract mRNA and DNA from FFPE tissue for use in pathology laboratories. Initially, the method will be used as part of a prognosis assay to predict the likelihood of distant metastasis and death for node-negative breast cancer patients.
Molekulare Forschung Deutschland, Siemens Healthcare Diagnostics, Nattermann Allee 1, 50829 Köln. Christoph.Petry@siemens.com
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Petry
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Gehrmann
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von Törne
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Weber
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Stropp
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Hennig
D000465:Algorithms; D014408:Biomarkers, Tumor / Q000235:genetics*; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D004273:DNA, Neoplasm / Q000235:genetics*; D004867:Equipment Design; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D020869:Gene Expression Profiling; D006801:Humans; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D016612:Paraffin Embedding; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation*; D012371:Robotics / Q000295:instrumentation*; D046888:Tissue Array Analysis / Q000295:instrumentation*
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D005404:Fixatives; D012333:RNA, Messenger; D005557:Formaldehyde
Prädiktive und prognostische Brustkrebsmarker. Molekularbiologische Analyse von fixiertem Tumorgewebe als Basis.