Application of pressure probe and UV-MALDI-TOF MS for direct analysis of plant underivatized carbohydrates in subpicoliter single-cell cytoplasm extract.
Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), and carbon nanotubes (CNTs) in positive ion mode were selected for analysis because of acceptable carbohydrate-related signal reproducibility. Disaccharide and oligosaccharide (up to 15 Hex when THAP was used, 11 Hex with DHB, and 7 Hex with CNTs) were detected in tulip bulb cell cytoplasm sample. When DHB was used as matrix, neutral carbohydrates were more abundantly detected as sodiated cations; the sugar-related signals, however, appeared as dominant potassiated cations when THAP and CNTs were used. Small amount of monosaccharide was also detected in bulb cell cytoplasm with CNTs as matrix. UV-MALDI-TOF MS of leaf cell extract resulted in high-resolution detection of hexose and disaccharide with DHB, THAP, and CNTs.
The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Japan.
Yousef
Y
Gholipour
Hiroshi
H
Nonami
Rosa
R
Erra-Balsells
D002241:Carbohydrates / Q000032:analysis*; D003593:Cytoplasm / Q000737:chemistry; D059828:Plant Cells; D010944:Plants / Q000737:chemistry*; D011312:Pressure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D031447:Tulipa / Q000737:chemistry / Q000166:cytology; D014466:Ultraviolet Rays
D002241:Carbohydrates