Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate.
In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis.
Deptartamento de Parasitologia, Laboratório de Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG. ana.paula@ufjf.edu.br
Ana Paula
AP
Ferreira
Thadeu
T
Côrrea
Rosângela
R
Cunha
Marcos José
MJ
Marques
Maria Angela
MA
Montesano
Maria Aparecida
MA
Souza
Henrique Couto
HC
Teixeira
D000208:Acute Disease; D000908:Antibodies, Fungal / Q000097:blood / Q000187:drug effects / Q000276:immunology*; D000937:Antigen-Antibody Reactions / Q000187:drug effects / Q000276:immunology; D000946:Antigens, Fungal / Q000097:blood / Q000187:drug effects / Q000276:immunology*; D015153:Blotting, Western; D016022:Case-Control Studies; D002908:Chronic Disease; D000939:Epitopes / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000097:blood / Q000187:drug effects / Q000276:immunology*; D008934:Mitogens / Q000627:therapeutic use; D010228:Paracoccidioides / Q000187:drug effects / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D010504:Periodic Acid / Q000627:therapeutic use
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D000939:Epitopes; D007132:Immunoglobulin Isotypes; D008934:Mitogens; D010504:Periodic Acid; C009288:metaperiodate