[Enrichment of saponins in Radix et Rhizoma Cynanchi Atrati with macroporous resin].
OBJECTIVE
To develop an approach to the determination of saponins in Radix Cynanchi Atrati, and to optimize the parameters for purified the preparation of total saponins by macroporous resin column chromatography.


METHODS
Using cynanversicoside A as a reference, the determination of saponins was performed; according to the elution rate and the purity of the products, the preparation performance of total saponins by macroporous resin was investigated, and its parameters were optimized.


RESULTS
The saponins in Radix Cynanchi Atrati were successfully determined at 518 nm by vanillin-perchloric acid as spray reagent. The macroporous resin HP-20 showed static absorption ratio of 59. 3 mg x g(-1); the 70% ethanol extraction of Radix Cynanchi Atrati was eluted from column of macroporous resin HP-20 by water and 30% ethanol, and the saponins were concentrated in 90% ethanol solution. The content of saponin part eluted from HP-20 column was 77.62%.


CONCLUSIONS
The proposed approach allows convenient and efficient preparation and purification of saponin in Radix Cynanchi Atrati.
Lv
Yong-hai
YH
School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Yang
Yun
Y
Yan
Shi-kai
SK
Liang
Ming-jin
MJ
Huang
Hao
H
Zhang
Wei-dong
WD
D000042:Absorption; D001547:Benzaldehydes / Q000737:chemistry; D002138:Calibration; D031105:Cynanchum / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D010472:Perchlorates / Q000737:chemistry; D016062:Porosity; D015203:Reproducibility of Results; D012116:Resins, Plant / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D001547:Benzaldehydes; D010472:Perchlorates; D012116:Resins, Plant; D012503:Saponins; D000431:Ethanol; C100058:vanillin