Synthesis and biological evaluation of the Zn (II)-IDB complexes appended with oligopolyamide as potent artificial nuclease.
The zinc (II) complexes, which contain oligopolyamide and bis(2-benzimidazolylmethyl)amine (IDB) conjugated by flexible linker, have been successfully synthesized, characterized, and evaluated as DNA cleavage agents. The cleavage activity of these complexes on DNA was studied by electrophoresis. The results showed that the cleavage activity of zinc (II) complexes was enhanced comparing with those without oligopolyamide. Specially, at a high reaction concentration (1.2mM), Zn (II) complex can cleave the plasmid DNA bearing some selectivity. Further, the spectroscopic data suggested that Zn (II) complexes with oligopolyamide backbone possessed A-T (adenine and thymine) rich sequences preference.
Department of Pharmaceutical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Chao
C
Li
Ren-Zhong
RZ
Qiao
Ya-Qin
YQ
Wang
Yu-Fen
YF
Zhao
Rong
R
Zeng
D000818:Animals; D001562:Benzimidazoles / Q000138:chemical synthesis* / Q000494:pharmacology*; D002417:Cattle; D004247:DNA / Q000737:chemistry; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D009757:Nylons / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D015032:Zinc / Q000737:chemistry*
D001562:Benzimidazoles; D009757:Nylons; D009942:Organometallic Compounds; D004247:DNA; D003850:Deoxyribonuclease I; D015032:Zinc