Boric acid-induced effects on protein profiles of Galleria mellonella hemolymph and fat body.
The dietary effects of boric acid (BA) on the protein profiles of greater wax moth, Galleria mellonella (L.), were investigated in hemolymph and fat body of final instar (VIIth) and pupae. The insects were reared from first-instar larvae on an artificial diets containing 156, 620, 1250 or 2500 ppm of BA. We detected many undetermined protein fractions (6.5-260 kDa) in addition to well-defined protein fractions such as lipophorins and storage proteins in the tissues by using sodium dodecyl-sulphate polyacrylamide gradient gel electrophoresis. A marked quantitative change in the 45 kDa protein fraction of the hemolymph was observed in the VIIth instar larvae reared on 2500 ppm dietary BA.
Department of Animal Physiology and Immunology, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, 611 37, Brno, Czech Republic. hyrsl@mail.muni.cz
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D000818:Animals; D001888:Boric Acids / Q000494:pharmacology*; D005216:Fat Body / Q000187:drug effects* / Q000378:metabolism*; D006458:Hemolymph / Q000187:drug effects* / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism*; D007915:Lepidoptera / Q000187:drug effects / Q000378:metabolism*
D001888:Boric Acids; D019476:Insect Proteins; C032688:boric acid