Effect of Radix isatidis polysaccharides on immunological function and expression of immune related cytokines in mice.
OBJECTIVE
To investigate the effect of Radix Isatidis polysaccharides (RIP) on the immunological function and expression of immune related cytokines in mice.


METHODS
Sixty mice were randomly divided into six groups, the normal group, cyclophosphamide (Cy) model group, Levamisole (positive control) group, RIP low, medium and high dose groups (0.08 g/kg, 0.16 g/kg, 0.32 g/kg, respectively), ten in each group. By detecting the value of abdominal macrophage phagocytic index, serum hemolysin (HC50), proliferation of lymphocytes and expression of related cytokines, interleukin (IL-2) and interferon gamma (INF-gamma), the effect of RIP on immunological function and its mechanism were studied.


RESULTS
RIP could improve the level of hemolysin in immunological function depressed mice. The results showed that the value of macrophage phagocytic index in the high dose RIP group increased from 1.11+/-0.13 to 1.42+/-0.26. The level of IL-2 and INF-gamma could be decreased by Cy to 38.12+/-6.88 ng/L and 139.23+/-29.87 ng/L, respectively, while RIP in high dose could increase the secretion of IL-2 and INF-gamma to 53.54+/-14.43 ng/L and 189.91+/-32.63 ng/L, respectively.


CONCLUSIONS
RIP could enhance non-specific immunological function, humoral immunity and cellular immunity in mice.
Zhao
Yan-ling
YL
Institute of Traditional Chinese Material Medica, the 302 Hospital of PLA, Beijing, 100039, China. zhao2855@263.com
Wang
Jia-bo
JB
Shan
Li-mei
LM
Jin
Cheng
C
Ma
Li
L
Xiao
Xiao-he
XH
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D003520:Cyclophosphamide / Q000494:pharmacology; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007109:Immunity / Q000187:drug effects*; D007165:Immunosuppression Therapy; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology
D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007376:Interleukin-2; D011134:Polysaccharides; D007371:Interferon-gamma; D003520:Cyclophosphamide