SSCP analysis of scnDNA for genetic profiling of Aedes aegypti.
We characterized genetic profiling markers for Aedes aegypti using single-strand conformation polymorphism (SSCP) analysis of single copy nuclear genes (scnDNA). Nucleotide variations at 18 loci were evaluated in 173 wild Ae. aegypti collected from a single population in northwestern Thailand. We identified seven scnDNAs with polymorphisms sufficient to determine a unique genetic profile for each mosquito examined. Six markers were derived from previously mapped cDNA loci. One marker was developed from a non-coding region of a gene. The number of alleles at each scnDNA locus ranged from 3 to 9. The described scnDNAs can be used to quickly fingerprint large numbers of Ae. aegypti to track the behavior of individual mosquitoes in the field.
Wong
Jacklyn
J
Department of Entomology, University of California, Davis, California 95616, USA. jacwong@ucdavis.edu
Tripet
Frédéric
F
Rasgon
Jason L
JL
Lanzaro
Gregory C
GC
Scott
Thomas W
TW
D000330:Aedes / Q000235:genetics* / Q000502:physiology; D000483:Alleles; D000818:Animals; D004247:DNA / Q000235:genetics*; D018628:Gene Dosage; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010058:Oviposition; D018807:Polymorphism, Single-Stranded Conformational
D005819:Genetic Markers; D004247:DNA