A hantavirus causing hemorrhagic fever with renal syndrome requires gC1qR/p32 for efficient cell binding and infection.
Hantaan virus (HTNV) is a pathogenic hantavirus that causes hemorrhagic fever with renal syndrome (HFRS). HTNV infection is mediated by alpha v beta3 integrin. We used protein blots of Vero E6 cell homogenates to demonstrate that radiolabeled HTNV virions bind to gC1qR/p32, the acidic 32-kDa protein known as the receptor for the globular head domain of complement C1q. RNAi-mediated suppression of gC1qR/p32 markedly reduced HTNV binding and infection in human lung epithelial A549 cells. Conversely, transient expression of either simian or human gC1qR/p32 rendered non-permissive CHO cells susceptible to HTNV infection. These results suggest an important role for gC1qR/p32 in HTNV infection and pathogenesis.
Choi
Yun
Y
Mogam Research Institute, 341 Pojungdong, Yongin, 449-910, Republic of Korea.
Kwon
Young-Chan
YC
Kim
Soo-In
SI
Park
Jung-Min
JM
Lee
Kyung-Hee
KH
Ahn
Byung-Yoon
BY
D000818:Animals; D019921:Biotinylation; D016466:CHO Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D004847:Epithelial Cells / Q000821:virology; D006476:Orthohantavirus / Q000378:metabolism* / Q000472:pathogenicity; D006480:Hemorrhagic Fever with Renal Syndrome / Q000378:metabolism* / Q000821:virology*; D006801:Humans; D015151:Immunoblotting; D008562:Membrane Glycoproteins / Q000378:metabolism*; D034622:RNA Interference; D011951:Receptors, Complement / Q000378:metabolism*; D014709:Vero Cells
D008562:Membrane Glycoproteins; D011951:Receptors, Complement; C032402:complement 1q receptor