Use of flow cytometry to monitor Legionella viability.
Legionella viability was monitored during heat shock treatment at 70 degrees C by a flow cytometric assay (FCA). After 30 min of treatment, for 6 of the 12 strains tested, the FCA still detected 10 to 25% of cells that were viable but nonculturable (VBNC). These VBNC cells were able to produce ATP and to be resuscitated after culture on amoebae.
Allegra
Séverine
S
Groupe Immunité des Muqueuses et Agents Pathogènes, EA3064, Faculté de Médecine J. Lisfranc, Université Jean Monnet, Saint-Etienne, France.
Berger
Françoise
F
Berthelot
Philippe
P
Grattard
Florence
F
Pozzetto
Bruno
B
Riffard
Serge
S
D000656:Amoeba / Q000382:microbiology; D000818:Animals; D005434:Flow Cytometry / Q000379:methods*; D006358:Hot Temperature; D007875:Legionella / Q000254:growth & development / Q000502:physiology* / Q000528:radiation effects; D050296:Microbial Viability