Alteration in testicular cell components following transiently induced ischaemia in prepubertal bulls.
The aim of the present study was to evaluate transient testicular ischaemia (induced using elastrator bands) in Jersey calves on testicular morphology and development. Treatments (at 27 +/- 5 days of age) consisted of control (0 h banding) and banding for 2, 4 or 8 h (n = 4 in each group). After castration (at 60 +/- 5 days of age), the right testis was used for calculation of cell components per testis according to the point-counting method. Bodyweight (59.8 +/- 6.2 kg) and scrotal circumference (SC) at banding (9.1 +/- 0.2 cm) did not differ between groups. Fresh testis weight, scrotal temperature immediately before band removal and daily SC growth were decreased in ischaemic (4 and 8 h) testes compared with controls (P < 0.05). In addition, the number of Sertoli and Leydig cells was significantly reduced in the 8 h ischaemic treatment group (P < 0.05). Transiently induced ischaemia significantly decreased the number of germ cells in the 8 h ischaemic treatment group (13 +/- 5 x 10(6) cells) compared with the 0, 2 and 4 h ischaemic treatment groups (38 +/- 6, 32 +/- 6 and 33 +/- 5 x 10(6) cells, respectively; P < 0.05). These results suggest that transiently induced ischaemia for 8 h significantly decreases the number of germ, Sertoli and Leydig cells in prepubertal testis.
Department of Animal Science, Institute of Agriculture, College of Veterinary Medicine, The University of Tennessee, Knoxville, TN 37996, USA.
G M
GM
Schuenemann
S M L C
SM
Mendis-Handagama
T M
TM
Prado
F N
FN
Schrick
D000818:Animals; D001831:Body Temperature; D002417:Cattle; D002452:Cell Count; D002467:Cell Nucleus / Q000473:pathology; D007511:Ischemia / Q000473:pathology* / Q000503:physiopathology; D007985:Leydig Cells / Q000473:pathology; D008297:Male; D012611:Scrotum / Q000503:physiopathology; D012708:Sertoli Cells / Q000473:pathology; D012741:Sexual Maturation; D013091:Spermatogenesis; D013094:Spermatozoa / Q000473:pathology; D013737:Testis / Q000098:blood supply* / Q000473:pathology* / Q000503:physiopathology