Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens.
Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are the three leading bacteria species associated with otitis media. Defining the molecular epidemiology of bacteria known to cause otitis media is of great importance, in both clinical and research settings. PFGE and MLST provide data for the characterization of isolates' genetic relatedness, yet they differ in the types of studies for which they are most useful. Consequently, knowledge of both techniques is important for laboratories intending to study the molecular epidemiology of otitis media-associated bacterial pathogens.
Yale University School of Public Health, New Haven, CT, USA.
Jonathan C
JC
Thomas
Melinda M
MM
Pettigrew
D001424:Bacterial Infections / Q000382:microbiology*; D015373:Bacterial Typing Techniques / Q000379:methods; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006193:Haemophilus influenzae / Q000235:genetics / Q000302:isolation & purification; D001936:Moraxella catarrhalis / Q000235:genetics / Q000302:isolation & purification; D010033:Otitis Media / Q000382:microbiology*; D017422:Sequence Analysis, DNA / Q000379:methods; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000302:isolation & purification