Lysine-enriched cecropin-mellitin antimicrobial peptides with enhanced selectivity.
Lysine-enriched analogs of the cecropin-mellitin hybrid peptide, CA(1-7) M(2-9) (designated CM15), designed with optimized amphipathicity, retained antimicrobial activities similar to that of wild-type CM15 and had substantially reduced levels of hemolytic activity and cytotoxicity toward cultured macrophages, resulting in enhanced selectivity. These lysine-enriched analogs provide templates for improved CM15 peptide or peptidomimetic antibiotics.
Sato
Hiromi
H
Department of Microbiology and Molecular Genetics and Center for Biopreparedness and Infectious Disease, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Feix
Jimmy B
JB
D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects*; D006461:Hemolysis; D006801:Humans; D008239:Lysine / Q000737:chemistry*; D008264:Macrophages; D051379:Mice; D008826:Microbial Sensitivity Tests; D013045:Species Specificity; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects*
D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; C513873:antimicrobial hybrid peptide CM15; D008239:Lysine