Array-based comparative genomic hybridization characterizes a deletion associated with a t(15;17) in acute promyelocytic leukemia.
The majority of de novo leukemias are characterized by well-known recurring translocations or inversions. In approximately 2% to 20% of these cases, deletions accompany these rearrangements. Because such deletions are undetectable by G-banding, aberrant fluorescence in situ hybridization (FISH) signal patterns are often the only indication of their presence. Array-based comparative genomic hybridization (a-CGH) permits examination of the entire genome at a resolution unattainable by G-banding or FISH. We present a case of a deletion of the derivative chromosome 17 of a t(15;17) in acute promyelocytic leukemia, the size and gene content of which were characterized by a-CGH. We hypothesize that this patient's more aggressive disease course is due to loss of one or more of these genes. Such submicroscopic deletions involving the t(15;17) have only rarely been reported, and, to our knowledge, this is the first case in which a-CGH has been applied to its characterization.
Department of Laboratory Medicine, Division of Hematology, Oncology and Transplantation, University of Minnesota Medical School, Minneapolis 55455, USA.
Michelle
M
Dolan
Bruce
B
Peterson
Betsy
B
Hirsch
D002871:Chromosome Banding; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D017809:Fatal Outcome; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012008:Recurrence; D017384:Sequence Deletion; D014178:Translocation, Genetic / Q000235:genetics*