[Study on isozymes in six species of Curcuma].
OBJECTIVE
To explore genetic relationships of the 39 materials in six species of Curcuma.


METHODS
The peroxidase isozyme (POD) and esterase isozyme (EST) were studied using vertical slab polyacrylamide gel electrophoresis (PAGE) technique, and the zymograms were analyzed using the software of NTSYSpc2. 1.


RESULTS
The interspecific zymogramatic differences were obvious. Each species possessed its own specific zymogram distinguishing form the others. In the analysis of EST isozyme, C. phaeocaulis, C. wenyujin, C. kwangsiensis and C. chuanhuangjiang had their own specific zymogram. In the analysis of POD isozyme, just C. phaeocaulis and C. kwangsiensis had their specific zymogram.


CONCLUSIONS
The genetic relationships are not associated with the geographical distributions and the genetic relationship between C. longa and C. sichuanensis are very close.
College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China.
Jia-yong
JY
Tang
Qing-miao
QM
Li
Rui-wu
RW
Yang
Jin-qiu
JQ
Liao
Yon-hong
YH
Zhou
D016000:Cluster Analysis; D030024:Curcuma / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000032:analysis* / Q000235:genetics; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics; D009195:Peroxidase / Q000032:analysis* / Q000235:genetics; D010802:Phylogeny; D013045:Species Specificity
D007527:Isoenzymes; D009195:Peroxidase; D004950:Esterases