Microarray analysis of acaricide-inducible gene expression in the southern cattle tick, Rhipicephalus (Boophilus) microplus.
Acaricide-inducible differential gene expression was studied in larvae of Rhipicephalus (Boophilus) microplus using a microarray-based approach. The acaricides used were: coumaphos, permethrin, ivermectin, and amitraz. The microarrays contained over 13 000 probes, having been derived from a previously described R. microplus gene index (BmiGI Version 2; Wang et al., 2007). Relative quantitative reverse transcriptase-PCR, real time PCR, and serial analysis of gene expression data was used to verify microarray data. Among the differentially expressed genes with informative annotation were legumain, glutathione S-transferase, and a putative salivary gland-associated protein.
USDA-ARS Knipling-Bushland US Livestock Insects Research Laboratory, Kerrville, TX 78028, USA.
L
L
Saldivar
F D
FD
Guerrero
R J
RJ
Miller
K G
KG
Bendele
C
C
Gondro
K A
KA
Brayton
D000818:Animals; D003372:Coumaphos / Q000633:toxicity; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D007559:Ivermectin / Q000633:toxicity; D020411:Oligonucleotide Array Sequence Analysis; D026023:Permethrin / Q000633:toxicity; D010575:Pesticides / Q000633:toxicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013987:Ticks / Q000235:genetics / Q000378:metabolism*; D014052:Toluidines / Q000633:toxicity
D017931:DNA Primers; D010575:Pesticides; D014052:Toluidines; C014983:amitraz; D026023:Permethrin; D007559:Ivermectin; D003372:Coumaphos