Validated high performance liquid chromatography-UV detection method for the determination of daptomycin in human plasma.
Daptomycin is the first approved member of the new class cyclic lipopeptide antibiotic drugs, effective against a broad spectrum of Gram-positive bacteria. Here we present an HPLC method with UV detection capable to obtain pharmacokinetic data of daptomycin in human plasma, exemplarily shown in a critically ill patient with acute renal failure undergoing extended daily dialysis. Sample preparation consists only of protein precipitation with methanol. Chromatographic separation was achieved on a Zorbax Eclipse XDB-C8 column and daptomycin was detected at 224 nm. The calibration function was linear over the range from 3.5 to 350 microg/ml. The relative standard deviations were <2% in the intra-day and <6% in the inter-day measurements. The accuracy was always better than 7%. Daptomycin was stable in aqueous solutions for 2 months frozen at-20 degrees C. However, in plasma frozen at -20 degrees C a loss of 25% in 1 month was observed.
Institute of Clinical Pharmacology, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, Germany. jens.martens-lobenhoffer@med.ovgu.de
Jens
J
Martens-Lobenhoffer
Jan T
JT
Kielstein
Catrin
C
Oye
Stefanie M
SM
Bode-Böger
D058186:Acute Kidney Injury / Q000188:drug therapy; D000328:Adult; D000900:Anti-Bacterial Agents / Q000097:blood / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017576:Daptomycin / Q000097:blood* / Q000627:therapeutic use; D004355:Drug Stability; D006801:Humans; D016014:Linear Models; D008297:Male; D006435:Renal Dialysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
D000900:Anti-Bacterial Agents; D017576:Daptomycin