Refinement of macaque synteny arrangement with respect to the official rheMac2 macaque sequence assembly.
We have compared the synteny block organization of the official macaque genome sequence assembly (Jan. 2006; rheMac2) with an independent assembly that used a molecular cytogenetic approach. The mapping of four synteny segments, ranging in size from 4 Mb to 24 Mb, was found to be inconsistent between the two datasets. We specifically investigated these discrepancies by appropriate co-hybridization FISH experiments with validated reference probes located outside the area under study. We found that in the macaque rheMac2 release three synteny segments were wrongly mapped and one segment was incorrectly oriented.
Roberto
Roberta
R
Department of Genetics and Microbiology, University of Bari, Via Amendola 165/A, 70126, Bari, Italy.
Misceo
Doriana
D
D'Addabbo
Pietro
P
Archidiacono
Nicoletta
N
Rocchi
Mariano
M
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial; D003582:Cytogenetics; D019143:Evolution, Molecular; D016678:Genome / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D006922:Hylobates / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008253:Macaca mulatta / Q000235:genetics*; D013045:Species Specificity; D026801:Synteny