Articulospora sp. produces Art1, an inhibitor of bacterial histidine kinase.
A two-component system (TCS) comprising a histidine kinase (HK) sensor and a response regulator (RR) plays important roles in regulating the virulence of many pathogenic bacteria. We used a new screening method to isolate novel inhibitor Art1 against bacterial sensory HK from an acetone extract of solid cultures of Articulospora sp., an aquatic hypomycete. Art1 inhibited the ATP-dependent autophosphorylation of recombinant glutathione S-transferase-fusion protein SasA, a cyanobacterial HK, with an IC50 value of 9.5 microg/ml.
Graduate School of Bioagricultural Science, Nagoya University, Nagoya, Japan.
Hirosuke
H
Sugahara
Tatsuhiko
T
Kondo
Masahiro
M
Okada
Yasuya
Y
Ikeda
Kenichi
K
Kaida
Ryosuke
R
Fudou
Takeshi
T
Mizuno
Youji
Y
Sakagami
D001203:Ascomycota / Q000737:chemistry / Q000378:metabolism*; D000458:Cyanobacteria / Q000201:enzymology*; D000071677:Histidine Kinase; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009284:Naphthols / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*
C534013:Art1 compound; D009284:Naphthols; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases; D000071677:Histidine Kinase