Liquid chromatography/mass spectrometry determination of endogenous plasma acetyl and palmitoyl carnitines as potential biomarkers of beta-oxidation in mice.
A robust bioanalytical method capable of measuring acetyl and palmitoyl carnitines was developed and validated. Application of hydrophilic interaction chromatography (HILIC) enabled retention of these highly polar and difficult to analyze compounds on a silica HPLC column. The chromatography was conducted with a high percentage of an organic component in the mobile phase, allowing high sensitivity for the pre-existing positively charged quaternary ammonium ions by electrospray ionization mass spectrometry. Successful application of the method to reliably quantify naturally occurring acyl carnitines in mouse plasma depended on the use of corresponding deuterated analogues. The specificity of the method, achieved through the use of stable isotope labeled compounds in combination with a mass spectral multiple reaction monitoring technique, permitted a non-invasive assessment of the overall change in the levels of these acyl carnitines in the plasma of intact animals administered peroxisome proliferator activated receptor (PPAR) agents. These acyl carnitines, as carriers of the corresponding long-chain fatty acids for transport into mitochondria, can be employed as potential biomarkers for significant alteration in the beta-oxidation process in an intact animal.
Drug Metabolism Department, Wyeth Research, Collegeville, PA 19426, USA. liup2@wyeth.com
Zhi
Z
Liu
Abdul E
AE
Mutlib
Jianyao
J
Wang
Rasmy E
RE
Talaat
D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D002138:Calibration; D002331:Carnitine / Q000031:analogs & derivatives* / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods*; D007553:Isotope Labeling; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010172:Palmitoylcarnitine / Q000097:blood* / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015415:Biomarkers; C116917:acylcarnitine; D010172:Palmitoylcarnitine; D002331:Carnitine