Electron tomography study of isolated human centrioles.
Centrioles are components of the centrosome, which is present in most eukaryotic cells (from protozoa to mammals). They organize the microtubule skeleton during interphase and the mitotic spindle during cell division. In ciliate cells, centrioles form basal bodies that are involved in cellular motility. Despite their important roles in biology, the detailed structure of centrioles remains obscure. This work contributes to a more complete model of centriole structure. The authors used electron tomography of isolated centrosomes from the human lymphoblast KE37 to explore the details of subdistal appendages and centriole lumen organization in mother centrioles. Their results reveal that each of the nine subdistal appendages is composed of two halves (20 nm diameter each) fused in a 40 nm tip that extends 100 nm from where it anchors to microtubules. The centriole lumen is filled at the distal domain by a 45 nm periodic stack of rings. Each ring has a 30 nm diameter, is 15 nm thick, and appears to be tilted at 53 degrees perpendicular to the centriole axis. The rings are anchored to microtubules by arms. Based on their results, the authors propose a model of the mother centriole distal structure.
Ibrahim
Rana
R
Institut Curie, Centre de Recherche, Orsay, F-91405 France.
Messaoudi
Cédric
C
Chichon
Francisco Javier
FJ
Celati
Claude
C
Marco
Sergio
S
D002453:Cell Cycle; D045744:Cell Line, Tumor; D002502:Centrioles / Q000648:ultrastructure*; D055032:Electron Microscope Tomography; D006801:Humans; D021621:Imaging, Three-Dimensional; D008958:Models, Molecular