Spectroscopic ultrahigh-resolution full-field optical coherence microscopy.
We have developed a full-field optical coherence microscopy system providing intensity-based tomographic images and spectroscopic information with ultrahigh spatial resolution. Local measurements of the backscattered light spectrum center of mass are achieved through short-time Fourier analysis of a stack of en face interferometric images acquired with a Linnik-type microscope. Using a halogen lamp as an illumination source enables us to achieve spectroscopic imaging over a wavelength range from 600 to 900 nm with a spatial resolution of approximately 1 microm. Absorption measurements of a colored gel are reported as a validation of the technique. Enhancement of tissue imaging contrast is demonstrated by imaging a Xenopus Laevis (African frog) tadpole ex vivo.
Laboratoire Charles Fabry de l'Institut d'Optique, CNRS UMR 8501, Université Paris-Sud, Campus Polytechnique, Palaiseau, France. arnaud.dubois@institutoptique.fr
Arnaud
A
Dubois
Julien
J
Moreau
Claude
C
Boccara
D003198:Computer Simulation; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008027:Light; D008853:Microscopy / Q000295:instrumentation*; D008962:Models, Theoretical; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation*; D041623:Tomography, Optical Coherence / Q000295:instrumentation*