Development of a high-performance liquid chromatographic method for the determination of a new potent radioiodinated melanoma imaging and therapeutic agent.
N-(2-diethylaminoethyl)-6-iodoquinoxaline-2-carbamide (ICF 01012) is a new melanoma imaging agent showing promising properties for application in internal radionuclide therapy. We developed an analytical protocol for detection of ICF 01012 in biological samples using HPLC. The proposed method was first validated using standard of ICF 01012 and four potent metabolites of this compound and then applied to follow the metabolic fate of [(125)I]ICF 01012 after injection in melanoma-bearing mice. The results demonstrate that this method exhibits a good linearity (r(2)=0.9947), specificity and acceptable accuracy. This simple method appears convenient and sufficient for pharmacokinetic studies on [(125)I]ICF 01012.
Denoyer
Delphine
D
EA 4231 INSERM-Université d'Auvergne-Centre Jean Perrin, BP 184, 63005 Clermont-Ferrand cedex, France. Delphine.Denoyer@petermac.org
Labarre
Pierre
P
Papon
Janine
J
Miot-Noirault
Elisabeth
E
Galmier
Marie-Josephe
MJ
Madelmont
Jean-Claude
JC
Chezal
Jean-Michel
JM
Moins
Nicole
N
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004195:Disease Models, Animal; D004355:Drug Stability; D007457:Iodine Radioisotopes / Q000378:metabolism; D016014:Linear Models; D008297:Male; D008546:Melanoma, Experimental / Q000000981:diagnostic imaging / Q000378:metabolism*; D051379:Mice; D009368:Neoplasm Transplantation; D011810:Quinoxalines / Q000378:metabolism*; D011877:Radionuclide Imaging; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D007457:Iodine Radioisotopes; C530303:N-(2-diethylaminoethyl)-6-iodoquinoxaline-2-carboxamide; D011810:Quinoxalines