Single base extension in multiplex blood group genotyping.
Transfusion recipients who become alloimmunized to blood group antigens require antigen-negative blood to limit adverse transfusion reactions. An alternative strategy to phenotyping blood is to assay genomic DNA for the associated single nucleotide polymorphisms (SNPs). A multiplex PCR coupled with a single base oligonucleotide extension assay using genomic DNA can identify SNPs related to D, C/c, E/e, S/s, K/k, Kp(a/b), Fy(a/b), Fy0 (-33 promoter silencing polymorphism), Jk(a/b), Di(a/b), and HPA-1a/b. Using this technology, individual SNP call rates vary from 98 to 100%. The platform has the capacity to genotype thousands of samples per day. The suite of SNPs provides rapid data for both blood donors and transfusion recipients and is poised to change whose blood is provided for potential transfusion recipients. The potential to dramatically lower the incidence of alloimmunization and to avoid serious hemolytic complications of transfusions can be realized with the implementation of this technology.
Research & Development Canadian Blood Service, Toronto, Canada.
Gregory A
GA
Denomme
D001789:Blood Group Antigens / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009841:Oligonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics
D001789:Blood Group Antigens; D009841:Oligonucleotides