Protective effect of aprotinin on ischemia-reperfusion injury in rat ovary.
OBJECTIVE
We investigated the effects of aprotinin on reperfusion injury in a controlled experimental rat torsion-detorsion model.


METHODS
Thirty-two female Sprague-Dawley rats were divided into four groups. Sham operation was performed in group I; in group II only ovarian torsion was performed. In group III, torsion-detorsion was performed, plus 3 mL/kg saline was injected i.v. 30 min before detorsion. In group IV, torsion-detorsion was performed, plus aprotinin (30,000 KIU/kg) was injected 30 min before detorsion. Rats in the torsion group were killed after 360 degrees clockwise adnexial torsion for 3 h, and the ovaries were harvested. After 3 h of adnexial detorsion, the rats in the saline and aprotinin groups were killed and the adnexa were surgically removed.


RESULTS
Ovarian tissue damage scores were significantly different among groups. Ovarian tissue and serum malondialdehyde levels in group III were significantly higher than those of groups I and IV (P<0.05). The serum levels of superoxide dismutase in group III were significantly lower than those of groups I and IV (P=0.01). Tissue and serum xanthine oxidase, nitric oxide, and tissue superoxide dismutase levels were comparable among groups (P>0.05).


CONCLUSIONS
Aprotinin attenuates ischemia-reperfusion injury in a rat adnexial torsion-detorsion model.
Department of Obstetrics and Gynecology, Faculty of Medicine, Afyonkarahisar Kocatepe University, Afyonkarahisar, Turkey. drfigenkir@yahoo.com
Figen Kir
FK
Sahin
Emine
E
Cosar
Gulengul
G
Koken
Hatice
H
Toy
Kemal
K
Basarali
Sadik
S
Buyukbas
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology*; D005260:Female; D006490:Hemostatics / Q000494:pharmacology*; D006651:Histocytochemistry; D010053:Ovary / Q000098:blood supply*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000517:prevention & control*
D006490:Hemostatics; D007611:Aprotinin