Lysine-enriched cecropin-mellitin antimicrobial peptides with enhanced selectivity.
Lysine-enriched analogs of the cecropin-mellitin hybrid peptide, CA(1-7) M(2-9) (designated CM15), designed with optimized amphipathicity, retained antimicrobial activities similar to that of wild-type CM15 and had substantially reduced levels of hemolytic activity and cytotoxicity toward cultured macrophages, resulting in enhanced selectivity. These lysine-enriched analogs provide templates for improved CM15 peptide or peptidomimetic antibiotics.
Department of Microbiology and Molecular Genetics and Center for Biopreparedness and Infectious Disease, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Hiromi
H
Sato
Jimmy B
JB
Feix
D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects*; D006461:Hemolysis; D006801:Humans; D008239:Lysine / Q000737:chemistry*; D008264:Macrophages; D051379:Mice; D008826:Microbial Sensitivity Tests; D013045:Species Specificity; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects*
D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; C513873:antimicrobial hybrid peptide CM15; D008239:Lysine