Expression of ALS1, HWP1 and SAP4 genes in Candida albicans strains isolated from women with vaginitis.
Total mRNA of Candida strains( isolated form whole vaginal swabs) was investigated and the in vivo expression of C. albicans secreted aspartyl proteinase (SAP4), aglutinin-like sequence (ALS1) and hyphal wall protein (HWP1) genes was determined. A spectrum of gene expression profiles of strain isolated from vulvovaginal candidiasis(VVC) cases consisting of 10 pregnant, 4 postmenopausal, and 15 reproductive age (12 primary and 3 recurrent) 9 women with different estrogen level. Expression of SAP4, ALS1 and HWP1 genes was evaluated by reverse-transcriptase polymerase chain reaction using specific primer sets. The expression of ALS1, HWP1, and SAP4 was detected as 69, 62, and 38 %, respectively, in all cases. In pregnant, postmenopausal, and reproductive age women with VVC, the expression of ALS1 was observed as 70, 75, 67%, and HWP 60, 25, 73% respectively . Expression of SAP4 was found in pregnant, postmenopausal, and reproductive age women as 40, 50, and 33% respectively. Expression of teh adhesion genes in VVC does not correlate with estrogen level of patients.
Department of Gynecology and Obstetrics, Gazi University, Ankara, Turkey.
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Nas
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Kalkanci
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Fidan
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Hizel
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Bolat
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Yolbakan
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Yilmaz
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Ozkan
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Kustimur
D016282:Aspartic Acid Endopeptidases / Q000235:genetics* / Q000378:metabolism; D002176:Candida albicans / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology*; D005260:Female; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D017698:Postmenopause; D011247:Pregnancy
C093538:ALS1 protein, Candida albicans; D005656:Fungal Proteins; C118102:HWP1 protein, Candida albicans; D008562:Membrane Glycoproteins; D016282:Aspartic Acid Endopeptidases; C086392:SAP4 protein, Candida albicans