[Diagnosis of Mycoplasma genitalium by MgPa and rRNA 16S gene amplification].
OBJECTIVE
Mycoplasma genitalium has been associated with nongonococcal urethritis (NGU). Diagnosis by PCR has become the primary detection method for this organism. Thus, diagnosis by DNA amplification using the PCR technique should be utilized.


METHODS
GMF/GMR and MgpF/MgpR primer pairs, complementary to the M. genitalium 16S rRNA and MgPa genes, respectively, were selected. Specificity and sensibility assays were conducted and clinical samples were studied.


RESULTS
The PCR with each primer pair was specific only for M. genitalium, and the sensibility was higher with the GMF/GMR primers. In the study of 34 clinical samples, 18,5% were positive for M. genitalium, with more positive samples when the MgpF/MgpR primers were used.


CONCLUSIONS
DNA amplification by PCR should be applied in clinical practice to the diagnosis of M. genitalium in patients with NGU should using.
Instituto de Medicina Tropical Pedro Kourí, La Habana, Cuba. carmen@ipk.sld.cu
Carmen
C
Fernández-Molina
Nadia
N
Rodríguez-Preval
Islay
I
Rodríguez-González
María
M
Agnese-Latino
José Antonio
JA
Rivera-Tapia
Idalia
I
Ayala-Rodríguez
D018829:Adhesins, Bacterial / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D006801:Humans; D008297:Male; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D045704:Mycoplasma genitalium / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D012680:Sensitivity and Specificity; D014526:Urethritis / Q000175:diagnosis* / Q000382:microbiology
D018829:Adhesins, Bacterial; D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C073268:adhesin, Mycoplasma genitalium
Diagnóstico de Mycoplasma genitalium por amplificación de los genes MgPa y ARN ribosomal 16S.