[Sensitivity and specificity of the VIKIA Rota-Adeno immuno-chromatographic test (bioMérieux) and the ELISA IDEIA Rotavirus kit (Dako) compared to genotyping].
The performances of two diagnostic tests for rotavirus infection in stool samples were evaluated during a prospective study in children of less than 36 months in child-care centers of Lyon from November 2004 to May 2005. The VIKIA Rota-Adeno immuno-chromatographic test (bioMérieux) and the ELISA IDEIA Rotavirus kit (Dako) were compared with a referral method, the genotyping. Fifty-seven stool samples were collected and analyzed by RT-PCR. The virus genome was detected in 29 samples. The most frequent genotypic combinations were G9P[8] with a prevalence of 75.9%. Sensitivity and specificity of the VIKIA Rota-Adeno test and the ELISA IDEIA Rotavirus kit were strictly comparable and very good: 96.6% (83.0; 99.9) and 96.4% (81.6; 99.9), respectively. The immuno-chromatographic technique were in concordance with the ELISA tests in 93.6% of cases. Thus, the VIKIA Rota-Adeno test is a good alternative for the occasional analysis of stool samples in ambulatory practice.
Centre national de référence des virus entériques, laboratoire de virologie, plateau technique de biologie, CHU de Dijon, 2, rue Angélique-Ducoudray, BP 37013, 21070 Dijon cedex, France.
A
A
de Rougemont
J
J
Kaplon
G
G
Billaud
B
B
Lina
S
S
Pinchinat
T
T
Derrough
E
E
Caulin
P
P
Pothier
D
D
Floret
D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D002656:Child Day Care Centers; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods; D003124:Colorimetry; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000821:virology; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005260:Female; D005602:France / Q000453:epidemiology; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007223:Infant; D008297:Male; D017921:Mastadenovirus / Q000235:genetics / Q000276:immunology; D011446:Prospective Studies; D012401:Rotavirus / Q000235:genetics / Q000276:immunology; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral
Sensibilité et spécificité du test immuno-chromatographique VIKIA Rota-Adéno (bioMérieux) et du kit Elisa IDEIA Rotavirus (Dako) par rapport aux techniques de génotypage.