Antispasmodic effect of hydroalcoholic extract of Thymus vulgaris on the guinea-pig ileum.
The effects of Thymus vulgaris hydroalcoholic extract on the contractile responses of the isolated guinea-pig ileum were investigated. Contraction changes in the terminal ileum of guinea pigs were monitored using a force displacement transducer amplifier connected to a physiograph. Thymus vulgaris extract inhibited the contractile responses in a dose-dependent manner and also decreased the amplitude of peristaltic waves. It is concluded that T. vulgaris has an antispasmodic action on guinea pig ileum by decreasing the amplitudes of the muscle contractions during peristalsis. The EC50 was calculated as 1.7 mg mL(-1). In guinea-pig ileum the extract led to an antispasmodic effect, possibly by affecting the anticholinergic and serotoninergic pathways.
Babaei
Mehdi
M
Internal Medicine Research Center, Semnan University of Medical Science, Semnan, Iran. amo_babaei@yahoo.com
Abarghoei
Mitra Emmami
ME
Ansari
Reza
R
Vafaei
Abbas Ali
AA
Taherian
Abbas Ali
AA
Akhavan
Maziar Mohammad
MM
Toussy
Gafar
G
Mousavi
Shahrokh
S
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D010276:Parasympatholytics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010528:Peristalsis / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D046930:Thymus Plant / Q000737:chemistry*; D014665:Vasodilator Agents / Q000494:pharmacology
D010276:Parasympatholytics; D010936:Plant Extracts; D014665:Vasodilator Agents; D000109:Acetylcholine