[Effects and mechanism of CD4+ CD25+ regulatory T cells in mouse experimental colitis treated by CLYSTER No. 1].
OBJECTIVE
To explore the effects and mechanism of CD4+ CD25+ regulatory T cells (Tregs) in mouse experimental colitis treated by CLYSTER No. 1.


METHODS
The mouse model of experimental colitis was established by dinitrochlorobenzene (DNCB)-acetic acid (AA) in mice DNCB and AA. Adult KM mouse were randomly divided into four groups: normal control group, experimental colitis model group, SASP and Chinese medicine therapeutic groups. Proportion of CD4 CD25+ Tregs in peripheral blood (PB) and mesenteric lymph node (MLN) was estimated by flow cytometry at the end of one or two week after treating with SASP and CLYSTER No. 1.


RESULTS
The model of experimental colitis in mouse was successfully established. Compared with normal control group, the proportion of CD4 CD25 Tregs was markedly decreased in PB and MLN of model control group of experimental colitis. But it was significantly increased in therapeutic groups of SASP and CLYSTER No. 1, and their CD4+ CD25+ Tregs in PB and MLN were much more than the model control group at the end of one or two weeks after treating with SASP and CLYSTER No. 1.


CONCLUSIONS
CD4+ CD25+ Tregs with strong immune suppression could play a central role in the initiation and development of mouse experiment colitis, and the CLYSTER No. 1 might exert its therapeutic effects on UC by the regulation of number and function of CD4+ CD25+ Tregs.
Department of Immunology, Institute of Radiation Medicine, AMMS, Beijing 100850, China.
Xiao-xia
XX
An
Yu-fang
YF
Cui
Ping
P
Liu
Yan
Y
Li
Bo
B
Dong
Shu-hua
SH
Sun
Xu-ni
XN
Shen
Xiao-lan
XL
Liu
Jian-ping
JP
Mao
D000818:Animals; D015704:CD4 Antigens / Q000276:immunology*; D003092:Colitis / Q000276:immunology*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D008297:Male; D051379:Mice; D011897:Random Allocation; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology*
D015704:CD4 Antigens; D004365:Drugs, Chinese Herbal; D053645:Interleukin-2 Receptor alpha Subunit