A waterbath method for preparation of RNA from Saccharomyces cerevisiae.
We have developed a simple and efficient method for the preparation of total RNA from Saccharomyces cerevisiae. Yeast cells were incubated at 65 degrees C for 5 min in yeast RNA isolation buffer (10 mM EDTA, 50mM Tris-HCl, 5% SDS, pH 6.0), and the RNA was isolated and purified. The yield and quality of the isolated RNA was consistently high, and the isolated RNA was suitable for downstream applications, such as Northern blot hybridization and reverse transcription PCR (RT-PCR).
Department of Medical Microbiology, Shandong University School of Medicine, Wenhua West Road, Jinan, Shandong 250012, China.
Jing
J
Li
Juan
J
Liu
Xin
X
Wang
Lei
L
Zhao
Qiang
Q
Chen
Weiming
W
Zhao
D012331:RNA, Fungal / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012331:RNA, Fungal