Purification and characterization of a thermal stable antimicrobial protein from housefly larvae, Musca domestica, induced by ultrasonic wave.
This work describes the induction, purification and partial biochemical characterizations of an antimicrobial protein from the housefly larvae induced by ultrasonic wave. It has been purified to apparent homogeneity by ammonium sulfate precipitation followed by Sephadex G-75, Bio-gel P6 gel filtration, and CM-Sepharose Fast Flow cation exchange chromatography. The protein is a cationic protein with an apparent molecular weight of 16315 Da determined by no-denaturing electrophoresis and SDS-PAGE, respectively. Biochemical profile assays show that this protein has good thermal stability, and repeatedly frozen and defrosted durability. The optimum pH for antimicrobial activity is around pH5. The antimicrobial range of the protein includes Gram-positive, Gram-negative bacteria and some fungi. Results of the membrane permeability assays suggest that the probable mode of action of this protein is membrane-disrupting mechanism.
Department of Cell and Genetics, Genetic Engineering Laboratory, College of Life Science, Nankai University, Tian Jin 300071, China. nkhyjin@hotmail.com
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Hao
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Jing
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Qu
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Li
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Du
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D005696:Galactosidases / Q000378:metabolism; D006793:Houseflies / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D019476:Insect Proteins / Q000302:isolation & purification* / Q000494:pharmacology*; D007814:Larva / Q000378:metabolism; D013696:Temperature; D014465:Ultrasonics
D000900:Anti-Bacterial Agents; D019476:Insect Proteins; D000469:Alkaline Phosphatase; D005696:Galactosidases