Response of cementoblast-like cells to mechanical tensile or compressive stress at physiological levels in vitro.
To clarify the role of cementoblast in orthodontic-related root resorption, this study was attempted to examine whether murine cementoblast-like cells are responsive to mechanical stress, and how mechanical forces regulate bone sialoprotein (BSP) and osteopontin (OPN) gene expression in these cells in vitro. In this force-loading model, defined and reproducible mechanical loadings of different magnitudes and types were applied up to 24 h. Besides a transitory and reversible change in cell proliferation, remarkable alterations in gene transcription of BSP and OPN were found. BSP mRNA was suppressed by the stresses. Three and six hours-loadings at 2,000 microstrain up-regulated the expression of OPN mRNA, while the other loadings inhibited it. The study also concluded that 4,000 microstrain was likely to exert more influence on cementoblast-like cells than 2,000 microstrain. Furthermore, no obvious evidence indicated the difference between tension and compression. These results suggested that cementoblast-like cells are sensitive to mechanical stress, and may play a role in regulating orthodontic-related root resorption/repair.
State Key Laboratory of Oral Disease, Sichuan University, Chengdu, People's Republic of China.
Lan
L
Huang
Yao
Y
Meng
Aishu
A
Ren
Xianglong
X
Han
Ding
D
Bai
Lina
L
Bao
D000818:Animals; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D019245:Compressive Strength; D003739:Dental Cementum / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation; D058574:Integrin-Binding Sialoprotein; D051379:Mice; D008858:Microscopy, Phase-Contrast; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism; D013314:Stress, Mechanical; D013718:Tensile Strength; D013997:Time Factors
C546872:Ibsp protein, mouse; D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D053495:Osteopontin