Chemical compositions responsible for inflammation and tissue damage in the mouse lung by coarse and fine particulate samples from contrasting air pollution in Europe.
Inflammation is regarded as an important mechanism in mortality and morbidity associated with exposures of cardiorespiratory patients to urban air particulate matter. We investigated the association of the chemical composition and sources of urban air fine (PM(2.5-0.2)) and coarse (PM(10-2.5)) particulate samples with the inflammatory activity in the mouse lung. The particulate samples were collected during selected seasons in six European cities using a high-volume cascade impactor. Healthy C57BL/6J mice were intratracheally instilled with a single dose (10 mg/kg) of the particulate samples. At 4, 12, and 24 h after the exposure, the lungs were lavaged and the bronchoalveolar lavage fluid (BALF) was assayed for indicators of inflammation and tissue damage: cell number, total protein, and cytokines (tumor necrosis factor [TNF]-alpha, interleukin [IL]-6, and KC). Dicarboxylic acids and transition metals, especially Ni and V, in PM(2.5-0.2) correlated positively and some secondary inorganic ions (NO3(-), NH4(+)) negatively with the inflammatory activity. Total organic matter and SO4(2-) had no consistent correlations. In addition, the soil-derived constituents (Ca2+, Al, Fe, Si) showed positive correlations with the PM(2.5-0.2)-induced inflammatory activity, but their role in PM(10-2.5) remained obscure, possibly due to largely undefined biogenic material. Markers of poor biomass and coal combustion, i.e., monosaccharide anhydrides and As, were associated with elevated PAH contents in PM(2.5-0.2) and a consistent immunosuppressive effect. Overall, our results support epidemiological findings that the local sources of incomplete combustion and resuspended road dust are important in urban air particulate pollution-related health effects.
Happo
Mikko S
MS
National Public Health Institute and University of Kuopio, Kuopio, Finland. Mikko.Happo@ktl.fi
Hirvonen
Maija-Riitta
MR
Halinen
Arja I
AI
Jalava
Pasi I
PI
Pennanen
Arto S
AS
Sillanpaa
Markus
M
Hillamo
Risto
R
Salonen
Raimo O
RO
D000393:Air Pollutants / Q000009:adverse effects* / Q000737:chemistry*; D000397:Air Pollution; D000818:Animals; D005060:Europe; D005648:Fuel Oils / Q000009:adverse effects; D008297:Male; D008670:Metals / Q000009:adverse effects / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009930:Organic Chemicals / Q000009:adverse effects / Q000737:chemistry; D010316:Particle Size; D052638:Particulate Matter / Q000009:adverse effects* / Q000737:chemistry*; D001335:Vehicle Emissions
D000393:Air Pollutants; D005648:Fuel Oils; D008670:Metals; D009930:Organic Chemicals; D052638:Particulate Matter; D001335:Vehicle Emissions