Serological proteome analysis of Staphylococcus aureus isolated from sub-clinical mastitis.
Staphylococcus aureus is the most common aetiologic agent of contagious bovine mastitis. Studies of the molecular epidemiology of S. aureus strongly suggest that some genetic subsets of strains are particularly well adapted for causing infections in cattle. This communication reports the setup of experimental protocols to identify the immunogenic proteins expressed by one of the most common field isolated strain of S. aureus responsible for sub-clinical mastitis cases. The serological proteome analysis (SERPA) approach applied consists of three main steps: two-dimensional electrophoresis-based separation of the proteins contained in field isolated S. aureus extracts enriched for surface proteins, detection of immunogenic spots using anti-serum collected from sub-clinical mastitis cases and identification of antigens by mass spectrometric-based methodologies. The study allowed to identify three immunogenic proteins: DNAase translocase FtsK, ribosomal proteins S1 and a Tell-like protein.
Tedeschi
Gabriella
G
Department of Animal Pathology, Hygiene and Health - University of Milano, via Celoria 10, 20133 Milano, Italy.
Taverna
Francesca
F
Negri
Armando
A
Piccinini
Renata
R
Nonnis
Simona
S
Ronchi
Severino
S
Zecconi
Alfonso
A
D000818:Animals; D002417:Cattle; D008414:Mastitis, Bovine / Q000382:microbiology*; D020543:Proteome / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism*
D020543:Proteome