Hypolipidemic effect of young persimmon fruit in C57BL/6.KOR-ApoEshl mice.
We investigated the hypolipidemic effects of young persimmon fruit (YP) on apolipoprotein E-deficient C57BL/6.KOR-ApoEshl mice. These mice exhibited higher plasma cholesterols, except for high-density lipoprotein (HDL), and lower plasma HDL cholesterol than C57BL/6.Cr mice that had the same genetic background as the C57BL/6.KOR-ApoEshl mice. Male C57BL/6.KOR-ApoEshl mice (n=5) were fed a diet supplemented with dry YP, Hachiya-kaki, at a concentration of 5% (w/w) for 10 weeks. YP treatment significantly lowered plasma chylomicron, very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) cholesterols, and triglyceride, and this response was accompanied by an elevation of fecal bile acid excretion. In the liver, sterol regulatory element binding protein-2 gene expression was significantly higher in mice fed YP, while the mRNA and protein levels of the LDL receptor did not change. These results indicate that acceleration of fecal bile acid excretion is a major mechanism of the hypolipidemic effect induced by YP in C57BL/6.KOR-ApoEshl mice.
Department of Applied Microbiology, Gifu Prefectural Research Institute for Bioengineering, Gifu, Japan. matsumoto@bio.rd.pref.gifu.jp
Kenji
K
Matsumoto
Shin-ichiro
S
Yokoyama
Nobuki
N
Gato
D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D029788:Diospyros / Q000737:chemistry* / Q000254:growth & development*; D005243:Feces; D005638:Fruit / Q000737:chemistry* / Q000254:growth & development*; D015870:Gene Expression; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
D001057:Apolipoproteins E; D001647:Bile Acids and Salts; D001786:Blood Glucose; D000960:Hypolipidemic Agents