Arterial gastroduodenal infusion of cholecystokinin-33 stimulates the exocrine pancreatic enzyme release via an enteropancreatic reflex, without affecting the endocrine insulin secretion in pigs.
OBJECTIVE
Cholecystokinin (CCK)-dependent exocrine pancreatic regulation seems to involve different pathways in different species. The aims were to explore the enteropancreatic reflex in the CCK-mediated regulation of the exocrine pancreas and to evaluate a possible involvement of this reflex in the endocrine insulin release.


METHODS
In anesthetized pigs, CCK-33 in increasing doses (4-130 pmol kg 10 min) was infused locally to the gastroduodenal artery, or systemically via the jugular vein. Also, a low CCK-33 dose (13 pmol kg) was injected to the duodenum/antrum area before and after a bilateral truncal vagotomy.


RESULTS
Cholecystokinin-33 in the physiological dose range 4 to 32 pmol kg 10 min increased protein and trypsin outputs after local infusion to the antral-duodenal area, whereas it had no effect after systemic infusion. Cholecystokinin-33 in the pharmacological dose range 64 to 130 pmol kg 10 min further increased the secretion after both local and systemic infusions. Only CCK-33 infusions in the pharmacological dose range were able to elevate the plasma insulin levels. Vagotomy had no effect on CCK-33-mediated stimulation of the enzyme release, whereas it had a significant effect on the plasma insulin level.


CONCLUSIONS
Cholecystokinin-33 in the physiological dose range 4 to 32 pmol kg 10 min stimulates the enzyme secretion but had no effect on the insulin release via a short enteropancreatic pathway in pigs.
Department of Cell and Organism Biology, Lund University, Lund, Sweden. Sofia.Rengman@cob.lu.se
Sofia
S
Rengman
Björn
B
Weström
Bo
B
Ahrén
Stefan G
SG
Pierzynowski
D000818:Animals; D002766:Cholecystokinin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004386:Duodenum / Q000187:drug effects* / Q000503:physiopathology / Q000637:transplantation; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D046790:Pancreas, Exocrine / Q000187:drug effects* / Q000201:enzymology; D012018:Reflex / Q000187:drug effects*; D013552:Swine; D014357:Trypsin / Q000378:metabolism; D014628:Vagotomy
D007328:Insulin; D002766:Cholecystokinin; D014357:Trypsin