Atypical one-carbon metabolism of an acetogenic and hydrogenogenic Moorella thermoacetica strain.
A thermophilic spore-forming bacterium (strain AMP) was isolated from a thermophilic methanogenic bioreactor that was fed with cobalt-deprived synthetic medium containing methanol as substrate. 16S rRNA gene analysis revealed that strain AMP was closely related to the acetogenic bacterium Moorella thermoacetica DSM 521(T) (98.3% sequence similarity). DNA-DNA hybridization showed 75.2 +/- 4.7% similarity to M. thermoacetica DSM 521(T), suggesting that strain AMP is a M. thermoacetica strain. Strain AMP has a unique one-carbon metabolism compared to other Moorella species. In media without cobalt growth of strain AMP on methanol was only sustained in coculture with a hydrogen-consuming methanogen, while in media with cobalt it grew acetogenically in the absence of the methanogen. Addition of thiosulfate led to sulfide formation and less acetate formation. Growth of strain AMP with CO resulted in the formation of hydrogen as the main product, while other CO-utilizing Moorella strains produce acetate as product. Formate supported growth only in the presence of thiosulfate or in coculture with the methanogen. Strain AMP did not grow with H(2)/CO(2), unlike M. thermoacetica (DSM 521(T)). The lack of growth with H(2)/CO(2) likely is due to the absence of cytochrome b in strain AMP.
Jiang
Bo
B
Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB, Wageningen, The Netherlands.
Henstra
Anne-Meint
AM
Paulo
Paula L
PL
Balk
Melike
M
van Doesburg
Wim
W
Stams
Alfons J M
AJ
D000085:Acetates / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny
D000085:Acetates; D002244:Carbon; D006859:Hydrogen; D000432:Methanol