Transcription expression and clinical significance of TTF-1 mRNA in pleural effusion of patients with lung cancer.
The aim of this study was to evaluate diagnostic utility of thyroid transcription factor 1 (TTF-1) mRNA in pleural effusions (PEs) of patients with lung cancer, especially primary pulmonary adenocarcinoma (PPA). Transcription levels of TTF-1 were detected by reverse transcription polymerase chain reaction (RT-PCR) in PEs of patients with lung cancer (56 cases) and with lung benign diseases (44 cases). The expression rate of TTF-1 mRNA was significantly higher in PEs of patients with lung cancer (73.2%) than with benign lung diseases (0%). In subclasses, the expression rate of TTF-1 mRNA was obviously higher in PEs of patients with PPA (93.0%) than with metastatic pulmonary adenocarcinoma (0%) and with primary pulmonary squamous cell carcinoma (12.5%). When compared with immunocytochemistry and cytology, TTF-1 mRNA gave the best diagnostic performance in diagnosing PPA: sensitivity of 93.0%, specificity of 100%, and accuracy of 96.6%, respectively. As a molecular marker of detecting pleural micrometastasis, TTF-1 mRNA may be helpful to diagnose the cancer cells in PEs of patients with lung cancer, especially with PPA.
Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang 110001, China.
Bing
B
Jiang
Guang-Ping
GP
Wu
Yu-Jie
YJ
Zhao
Shao-Cheng
SC
Wang
D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D016066:Pleural Effusion, Malignant / Q000175:diagnosis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012680:Sensitivity and Specificity; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D014408:Biomarkers, Tumor; C000614412:NKX2-1 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors