Neural stem cell differentiation is mediated by integrin beta4 in vitro.
Neural stem cells are capable of differentiating into three major neural cell types, but the underlying molecular mechanisms remain unclear. Here, we investigated the mechanism by which integrin beta4 modulates mouse neural stem cell differentiation in vitro. Inhibition of endogenous integrin beta4 by RNA interference inhibited the cell differentiation and the expression of fibroblast growth factor receptor 2 but not fibroblast growth factor receptor 1 or fibroblast growth factor receptor 3. Overexpression of integrin beta4 in neural stem cells promoted neural stem cell differentiation. Furthermore, integrin beta4-induced differentiation of neural stem cells was attenuated by SU5402, the inhibitor of fibroblast growth factor receptors. Finally, we investigated the role of integrin beta4 in neural stem cell survival: knockdown of integrin beta4 did not affect survival or apoptosis of neural stem cells. These data provide evidence that integrin beta4 promotes differentiation of mouse neural stem cells in vitro possibly through fibroblast growth factor receptor 2.
Su
Le
L
Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
Lv
Xin
X
Xu
Jiping
J
Yin
DeLing
D
Zhang
HaiYan
H
Li
Yi
Y
Zhao
Jing
J
Zhang
ShangLi
S
Miao
JunYing
J
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D039663:Integrin beta4 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000166:cytology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*; D014162:Transfection
D039663:Integrin beta4; D034741:RNA, Small Interfering; D017468:Receptors, Fibroblast Growth Factor