[Coumarins from branch of Fraxinus sieboldiana and their antioxidative activity].
OBJECTIVE
To investigate the chemical constituents from the branch of Fraxinus sieboldiana, and evaluate their antioxidative activity.


METHODS
The chemical constituents were isolated and purified by chromatographic techniques over silica gel, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC. Structures of the compounds were identified by spectroscopic methods. The antioxidant activity was evaluated by Fe(+2)-cystine induced rat liver microsomal lipid peroxidation.


RESULTS
Eight coumarins were obtained and their structures were elucidated as esculin (1) , esculetin (2), fraxin (3), fraxetin (4), 6, 7-di-O-beta-D-glucopyranosylesculetin (5), scopoletin (6), cleomiscosin D (7) and cleomiscosin B (8). At a concentration of 10(-6) mol x L(-1), compound 4 showed antioxidative activity inhibiting Fe(+2)-cystine induced rat liver microsomal lipid peroxidation with inhibitory rate of 60%.


CONCLUSIONS
Compounds 5, 7 and 8 were obtained from the genus Fraxinus for the first time. Compound 4 showed remarkable antioxidative activity, which was higher than that of VE (35%).
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Sheng
S
Lin
Ming-tao
MT
Liu
Su-juan
SJ
Wang
Shuai
S
Li
Yong-chun
YC
Yang
Jian-gong
JG
Shi
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology; D031661:Fraxinus / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D012603:Scopoletin / Q000737:chemistry / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D014468:Umbelliferones / Q000737:chemistry / Q000494:pharmacology
D000975:Antioxidants; D003374:Coumarins; D014468:Umbelliferones; C438426:cleomiscosin D; C105671:fraxetin; D012603:Scopoletin; C007628:esculetin; C080614:fraxin