Enhanced structural and functional genome elucidation of the arsenite-oxidizing strain Herminiimonas arsenicoxydans by proteomics data.
The arsenite-oxidizing strain Herminiimonas arsenicoxydans proteome was investigated with gel electrophoresis and tandem mass spectrometry analyses. The comparison of experimental and theoretical M(r) and pI, as well as that of peptide sequences identified by MS and predicted protein sequences, allowed the correction of five protein annotations. More importantly, the functional analysis of SDS- and 2D-PAGE proteome maps obtained in the presence of arsenic, combined with partial transcriptomic results indicate that H. arsenicoxydans expressed genes and proteins required not only for arsenic detoxification or stress response but also involved in motility, exopolysaccharide synthesis, phosphate import or energetic metabolism. This study provides therefore new insights into the adaptation processes of H. arsenicoxydans in response to arsenic.
Weiss
Stéphanie
S
Génétique Moléculaire, Génomique et Microbiologie, Université Louis Pasteur, Strasbourg, France.
Carapito
Christine
C
Cleiss
Jessica
J
Koechler
Sandrine
S
Turlin
Evelyne
E
Coppee
Jean-Yves
JY
Heymann
Michaël
M
Kugler
Valérie
V
Stauffert
Magalie
M
Cruveiller
Stéphane
S
Médigue
Claudine
C
Van Dorsselaer
Alain
A
Bertin
Philippe N
PN
Arsène-Ploetze
Florence
F
D018053:Arsenites / Q000378:metabolism*; D016680:Genome, Bacterial; D008954:Models, Biological; D042802:Oxalobacteraceae / Q000235:genetics* / Q000378:metabolism*; D010084:Oxidation-Reduction; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
D018053:Arsenites; D020543:Proteome; C015001:arsenite