In vivo real-time imaging of nuclear-cytoplasmic dynamics of dormancy, proliferation and death of cancer cells.
The in vivo visualization of dormancy, proliferation, as well as cell death is of great importance in understanding the cellular pathology of cancer. We describe here the use of fluorescent proteins to differentially label cells in the nucleus and cytoplasm, and high-powered imaging technology to visualize the nuclear-cytoplasmic dynamics of these phenomena of cancer in vivo. The implications of this technology for discovery of new visible targets at the cellular level for treatment of cancer are discussed.
Hoffman
Robert M
RM
AntiCancer, Inc. and Department of Surgery, University of California at San Diego, San Diego, CA 92111-3604, USA. all@anticancer.com
D021581:Active Transport, Cell Nucleus; D000818:Animals; D002453:Cell Cycle; D016923:Cell Death; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism / Q000473:pathology; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D009374:Neoplasms, Experimental / Q000098:blood supply / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins