Gene expression profiles of rat olfactory bulb at developmental stage.
Microarray analysis may be a useful tool to identify some candidate genes related to the development of olfactory bulbs. In the present study, gene expression profiles of olfactory bulbs from postnatal day 1 (P1) rats and postnatal day 35 (P35) rats were analyzed by oligonucleotide-microarray and expression levels of some selected genes were also confirmed by RT-PCR and in situ hybridization. 9146 genes were commonly identified in six microarray chips. Among these genes, 76 were up-regulated and 130 were down-regulated three-folds or more at P1 olfactory bulbs. Out of these 76 up-regulated genes, 24 genes were annotated based on the NCBI database of reference sequences and expression levels of these 24 genes were confirmed by RT-PCR. Among them, 2 interesting genes (neurogenic differentiation 1 and retinoid acid receptor alpha) were localized in the P1 olfactory bulb by the use of in situ hybridization technique. Our results may provide basic information to identify genes associated with functional growth of olfactory bulbs.
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Kon-Kuk University, Seoul, South Korea.
Jae Hoon
JH
Cho
Hak Hyun
HH
Jung
Sang Hak
SH
Lee
Gi Jung
GJ
Im
Jiwon
J
Chang
Seo Jin
SJ
Kim
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D009830:Olfactory Bulb / Q000254:growth & development* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D051381:Rats; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism; D000072482:Retinoic Acid Receptor alpha; D014158:Transcription, Genetic / Q000502:physiology*
D051792:Basic Helix-Loop-Helix Proteins; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; C501008:Neurod1 protein, mouse; C000607072:Rara protein, mouse; C000607073:Rara protein, rat