[HPLC determination of 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine in Nelumbo nucifera and its alkaloid fraction].
OBJECTIVE
To establish an HPLC method for the determination of four alkaloids, i.e., 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine, in Nelumbo nucifera and its alkaloid fraction.


METHODS
The determination was carried out at 35 degrees C on a Hypersil C18 column (4.6 mm x 250 mm, 5 microm), eluting with acetonitrile-water containing 0.1% triethylamine as mobile phases in gradient mode. The flow rate was 1.0 mL x min(-1) and detection at the wavelength was set at 270 nm.


RESULTS
The linear ranges of 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine were 0.110-0.658 microg (r = 0.9995), 0.0210-0.126 microg (r = 0.9995), 0.103-0.618 microg (r = 0.9998), 0.085 6-0.514 microg (r = 0.9995), with the average recoveries (n=6) were 101.5%, 99.14%, 99.21% and 98.41% for the alkaloid fraction of N. nucifera and 99.53%, 100.5%, 97.51% and 100.1% for N. nucifera respectively.


CONCLUSIONS
The determination results of the three batches of samples showed that the method was easy and accurate which could be used to determine the contents of four components in N. nucifera and its alkaloid fraction.
Wang
Yu-xia
YX
Bejing University of Chinese Medicine, Beijing 100102, China.
Liu
Bin
B
Shi
Ren-bing
RB
D000470:Alkaloids / Q000737:chemistry; D001060:Aporphines / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031653:Nelumbo / Q000737:chemistry*; D013141:Spiro Compounds / Q000737:chemistry
D000470:Alkaloids; D001060:Aporphines; D004365:Drugs, Chinese Herbal; D013141:Spiro Compounds; C081279:aporphine; C030169:roemerine; C514305:pronuciferine; C008692:nuciferine