Delivery and visualization of proteins conjugated to quantum dots in cardiac myocytes.
The design of a novel transduction complex has permitted the introduction of protein-quantum dot conjugates into the cytoplasm of living cells. Appropriate subcellular localization of quantum dot-conjugated cardiac troponin C to the myofibrils and a nuclear peptide to the nucleus was attained in living cardiac myocytes using this approach. This new methodology opens the possibility for live tracking of exogenous proteins and study of protein dynamics.
Koshman
Yevgeniya E
YE
Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA.
Waters
Stephen B
SB
Walker
Lori A
LA
Los
Tamara
T
de Tombe
Pieter
P
Goldspink
Paul H
PH
Russell
Brenda
B
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism; D045663:Quantum Dots; D051381:Rats; D019209:Troponin C / Q000378:metabolism* / Q000494:pharmacology
D019209:Troponin C