CD24 and CD70 as differential markers of human hepatocellular carcinoma cells in culture.
We developed a new method for evaluation of the purity of cell population in primary cultures of hepatocellular carcinoma. Expression of potential surface molecular markers on primary cultures of renal tumors was assayed by the method of flow cytofluorometry. CD24 and CD70 were identified as differential markers, which allowed us to distinguish cancer cells from tumor stromal cells in vitro. A negative marker CD90 was expressed on fibroblasts of the tumor stroma, but not on cancer cells. Combined use of these three markers allows evaluation of the severity of tumor culture contamination with stromal cells.
Karalkin
P A
PA
Russian State Medical University, Moscow. bio_cell@mail.ru
Kovtun
N E
NE
Lupatov
A Yu
AY
Kurynin
R V
RV
Chalyi
M E
ME
Aliaev
I G
IG
Yarygin
K N
KN
D015703:Antigens, CD / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D051927:CD24 Antigen / Q000378:metabolism*; D053282:CD27 Ligand / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D014408:Biomarkers, Tumor; D051927:CD24 Antigen; C497889:CD24 protein, human; D053282:CD27 Ligand; C505771:CD70 protein, human; C475476:LRP1 protein, human; D026503:Low Density Lipoprotein Receptor-Related Protein-1