Safety and efficacy testing of a novel multivalent bovine bacterial respiratory vaccine composed of five bacterins and two immunogens.
Bovine bacterial respiratory diseases have been one of the most serious problems due to their high mortality and economic loss in calves. The vaccinations of bovine bacterial respiratory vaccines have been complex because of no multivalent vaccine. In this study, novel multivalent bovine bacterial respiratory vaccine (BRV) was developed and tested for its safety and efficacy. BRV was composed of two immunogens and five bacterins. These were leukotoxoid and bacterin of Mannheimia haemolytica type A, outer membrane protein and bacterin of Pasteurella multocida type A, and bacterins of Haemophilus somnus, Mycoplasma bovis, and Arcanobacterium pyogenes. ELISA antibody titers to five bacterial antigens in vaccinated guinea pigs increased, compared with those in unvaccinated ones. BRV was safe for calves and pregnant cattle in this study. In calves challenged with M. haemolytica and P. multocida, the average daily weight gain and antibody titers of vaccinated calves increased, and respiratory symptoms (P<0.05) and treatment frequency (P<0.01) of vaccinated calves significantly decreased, compared with those of unvaccinated calves. Interestingly, the antibody titers of M. haemolytica leukotoxoid and Mycoplasma bovis were closely related with the reduction of respiratory symptoms. BRV would be an ecomonical measure for the protection against bovine bacterial respiratory diseases.
Cho
Yun Sang
YS
Bacteriology and Parasitology Division, Animal Disease Research Department, National Veterinary Research & Quarantine Service, Anyang, Kyounggi, Republic of Korea. choys@nvrqs.go.kr
Lee
Hee Soo
HS
Lim
Sook-Kyoung
SK
Joo
Yi-Seok
YS
Kim
Jong Man
JM
Kim
Jae Hoon
JH
D000818:Animals; D055667:Arcanobacterium / Q000276:immunology; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D044138:Haemophilus somnus / Q000276:immunology; D016978:Mannheimia haemolytica / Q000276:immunology; D051379:Mice; D045682:Mycoplasma bovis / Q000276:immunology; D016979:Pasteurella multocida / Q000276:immunology; D012120:Respiration Disorders / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D017778:Vaccines, Combined
D001428:Bacterial Vaccines; D017778:Vaccines, Combined