Expression of livin and vascular endothelial growth factor in different clinical stages of human esophageal carcinoma.
OBJECTIVE
To investigate the role of livin and vascular endothelial growth factor (VEGF) in human esophageal carcinoma, and analyze its relationship to clinical stages.


METHODS
Expression of livin in fresh esophageal cancer tissues was detected by immunohistochemistry (IHC), Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR), and VEGF by Western blotting and RT-PCR. All statistical analyses were performed by SPSS version 11.0.


RESULTS
Livin positivity was also significantly correlated with tumor stages, increasing with tumor progression. Expression of livin and VEGF increased with the process of esophageal carcinoma. In the fourth clinical stage, expression of livin and VEGF was the most significant. Expression of Livin was positively correlated with VEGF.


CONCLUSIONS
Over-expression of livin and VEGF contributes to the pathogenesis of esophageal carcinoma.
Department of General Surgery, the First Affiliated Hospital of Chongqing Medical Uni-versity, Chongqing 400016, China.
Li
L
Chen
Guo-Sheng
GS
Ren
Fan
F
Li
Shan-Quan
SQ
Sun
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis* / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002277:Carcinoma / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D004938:Esophageal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000032:analysis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; C419866:BIRC7 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A