Neutrophils and monocytes transport tumor cell antigens from the peritoneal cavity to secondary lymphoid tissues.
Antigen-transporting cells take up pathogens, and then migrate from sites of inflammation to secondary lymphoid tissues to induce an immune response. Among antigen-transporting cells, dendritic cells (DCs) are believed to be the most potent and professional antigen-presenting cells that can stimulate naïve T cells. However, the cells that transport antigens, tumor cell antigens in particular, have not been clearly identified. In this study we have analyzed what types of cells transport tumor cell antigens to secondary lymphoid tissues. We show that neutrophils, monocytes and macrophages but not DCs engulf X-irradiated P388 leukemic cells after their injection into the peritoneal cavity, and that neutrophils and monocytes but not macrophages migrate to the parathymic lymph nodes (pLN), the blood, and then the spleen. The monocytes in the pLN comprise Gr-1(-) and Gr-1(+) ones, and some of these cells express CD11c. Overall, this study demonstrates that neutrophils and monocytes transport tumor cell antigens from the peritoneal cavity to secondary lymphoid tissues.
Terasawa
Masao
M
Division of Molecular Medicine, Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1, Miyama, Funabashi, Japan.
Nagata
Kisaburo
K
Division of Molecular Medicine, Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1, Miyama, Funabashi, Japan.
Kobayashi
Yoshiro
Y
Division of Molecular Medicine, Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1, Miyama, Funabashi, Japan. Electronic address: yoshiro@biomol.sci.toho-u.ac.jp.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D008198:Lymph Nodes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009000:Monocytes / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D010529:Peritoneal Cavity; D021381:Protein Transport; D019707:Receptors, Chemokine / Q000235:genetics; D013154:Spleen / Q000276:immunology*
D000951:Antigens, Neoplasm; C503133:Gr-1 protein, mouse; D019707:Receptors, Chemokine