In vitro investigation of oxide nanoparticle and carbon nanotube toxicity and intracellular accumulation in A549 human pneumocytes.
If released in the environment, nanomaterials might be inhaled by populations and cause damage to the deepest regions of the respiratory tract, i.e., the alveolar compartment. To model this situation, we studied the response of A549 human pneumocytes after exposure to aluminium oxide or titanium oxide nanoparticles, and to multi-walled carbon nanotubes. The influence of size, crystalline structure and chemical composition was investigated. After a detailed identification of nanomaterial physico-chemical characteristics, cells were exposed in vitro and viability and intracellular accumulation were assessed. In our conditions, carbon nanotubes were more toxic than metal oxide nanoparticles. Our results confirmed that both nanotubes and nanoparticles are able to rapidly enter into cells, and distribute in the cytoplasm and intracellular vesicles. Among nanoparticles, we demonstrate significant difference in biological response as a function of size, crystalline phase and chemical composition. Their toxicity was globally lower than nanotubes toxicity. Among nanotubes, the length did not influence cytotoxicity, neither the presence of metal catalyst impurities.
Laboratoire Pierre Süe, CEA-CNRS UMR9956, IRAMIS, CEA Saclay, 91191 Gif sur Yvette, France.
A
A
Simon-Deckers
B
B
Gouget
M
M
Mayne-L'hermite
N
N
Herlin-Boime
C
C
Reynaud
M
M
Carrière
D000537:Aluminum Oxide / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000187:drug effects* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008168:Lung / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D053768:Metal Nanoparticles / Q000032:analysis / Q000737:chemistry / Q000633:toxicity*; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000032:analysis / Q000737:chemistry / Q000633:toxicity*; D020545:Respiratory Mucosa / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D014025:Titanium / Q000633:toxicity
D037742:Nanotubes, Carbon; C009495:titanium dioxide; D014025:Titanium; D000537:Aluminum Oxide