In vitro generation of three-dimensional renal structures.
End-stage renal disease is currently being treated effectively by transplantation. However, increasing demand and donor shortage make this treatment challenging. Recent advances in cell-based therapies have provided potential opportunities to alleviate the current challenges of donor shortage. In this study we developed a system to generate renal structures in vitro using primary kidney cells. This system involves the cultivation of expanded primary renal cells in a three-dimensional collagen-based culture system. After one week of growth, individual renal cells began to form renal structures resembling tubules and glomeruli. Histologically, these structures show phenotypic resemblance to native kidney structures. The reconstituted tubules stained positively for Tamm-Horsfall protein, which is expressed in the thick ascending limb of Henle's Loop and distal convoluted tubules. These results show that renal structures can be reconstituted in a three-dimensional culture system, which may eventually be used for renal cell therapy applications.
Joraku
Akira
A
Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, 391 Technology Way, Winston-Salem, NC 27157, USA.
Stern
Kathryn A
KA
Atala
Anthony
A
Yoo
James J
JJ
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003094:Collagen; D007668:Kidney / Q000166:cytology*; D007676:Kidney Failure, Chronic / Q000628:therapy; D007686:Kidney Tubules, Distal / Q000166:cytology; D008138:Loop of Henle / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009088:Mucoproteins / Q000096:biosynthesis; D023822:Tissue Engineering / Q000379:methods*; D058949:Uromodulin
D009088:Mucoproteins; C548613:Umod protein, mouse; D058949:Uromodulin; D003094:Collagen