The effects of superoxide dismutase knockout on the oxidative stress parameters and survival of mouse erythrocytes.
The erythrocytes of 12-month old Sod1 (-/-) mice showed an increased level of reactive oxygen species (ROS), as estimated by the degree of dihydroethidine and dihydrorhodamine oxidation, and the increased level of Heinz bodies. No indices of severe oxidative stress were found in the red blood cells and blood plasma of Sod1 (-/-) mice as judged from the lack of significant changes in the levels of erythrocyte and plasma glutathione, plasma protein thiol and carbonyl groups and thiobarbituric-acid reactive substances in the blood plasma. However, a decreased erythrocyte lifespan, increased reticulocyte count and splenomegaly were noted, indicating the importance of superoxide dismutase for maintaining erythrocyte viability. The levels of erythrocyte ROS and Heinz bodies and the reticulocyte count were indistinguishable in Sod1 (+/+) and Sod1 (+/-) mice, suggesting that a superoxide dismutase activity decrease to half of its normal value may be sufficient to secure the protective effects of the enzyme.
Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Agnieszka
A
Grzelak
Marcin
M
Kruszewski
Ewa
E
Macierzyńska
Łukasz
Ł
Piotrowski
Łukasz
Ł
Pułaski
Błazej
B
Rychlik
Grzegorz
G
Bartosz
D000818:Animals; D002470:Cell Survival; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000166:cytology* / Q000201:enzymology*; D006366:Heinz Bodies / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009929:Organ Size; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000097:blood; D013154:Spleen / Q000033:anatomy & histology; D013482:Superoxide Dismutase / Q000172:deficiency* / Q000378:metabolism
D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase