Flavobacterium resistens sp. nov., isolated from stream sediment.
An aerobic, yellow-pigmented, Gram-negative bacterium, designated strain BD-b365(T), was isolated from sediment of the Hakjang stream in Busan, South Korea. Growth was observed at 15-40 degrees C (optimum 20-30 degrees C) and at pH 6.0-9.5 (optimum pH 7.0-8.0). Cells were non-spore-forming rods that showed gliding motility and contained branched and hydroxy fatty acids. The G+C content of the genomic DNA was 35.4 mol%. The major respiratory quinone was menaquinone-6 (MK-6). The major polar lipid of strain BD-b365(T) was phosphatidylethanolamine. Comparative 16S rRNA gene sequence analysis showed that strain BD-b365(T) formed a distinct phyletic line within the genus Flavobacterium. Based on levels of 16S rRNA gene sequence similarity, the novel strain was related most closely to Flavobacterium aquidurense WB 1.1-56(T), but the level of DNA-DNA relatedness between these two strains was only 9.6 %. On the basis of phenotypic and genotypic data, it is clear that strain BD-b365(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium resistens sp. nov. is proposed. The type strain is BD-b365(T) (=KCTC 22078(T) =DSM 19382(T)).
Ryu
Seung Hyun
SH
Division of Applied Life Science (BK21 program), EB-NCRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Park
Jin Ho
JH
Moon
Jeong Chan
JC
Sung
Youlboong
Y
Lee
Sang-Suk
SS
Jeon
Che Ok
CO
D015373:Bacterial Typing Techniques; D001482:Base Composition; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D020459:Genes, rRNA; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology; D007723:Korea; D008969:Molecular Sequence Data; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S