[The role of hydrogen sulfide and cystathionine-gamma-lyase in allergic rhinitis guinea pigs].
OBJECTIVE
To study the change of endogenous hydrogen sulfide (hydrogen sulfide, H2S) and its rate-limiting enzyme Cystathionine-gamma-lyase (CSE) in allergic rhinitis through guinea pigs with intervention treatment.


METHODS
Twenty-four guinea pigs were divide into 4 groups at random, one group were models of allergic rhinitis (AR) which were established by using ovalbumin, the second group were treated with NaHS after sensitized, the third group were treated with Propargylglycine (PPG) which was suppression of CSE after sensitized, and the last group were treated with saline for control. The concentration of eotaxin of nasal lavage and H2S in plasma were recorded, and then the expression of CSE in nasal mucosa was determined by real-time fluorescence RT-PCR.


RESULTS
The concentration of eotaxin in nasal lavage of sensitized group were higher than those of control (P < 0.01), and concentration of H2S in plasma and expression of CSE in nasal mucosa were lower than control (P < 0.05). The concentration of eotaxin decreased when treated with NaHS and increased when treated with PGG (P < 0.05). Level of H2S in plasma and expression of CSE increased when treated with NaHS and decreased when treated with PGG (P < 0.05), and the level of H2S was positive linear correlate with the expression of CSE.


CONCLUSIONS
Endogenous H2S perhaps plays a significant role in the pathogenesis of allergic rhinitis, and it was mainly regulated by CSE.
Yu
Shaoqing
S
Department of Otolaryngology, Jinan General Hospital of PLA, Shandong, 250031, China.
Zhang
Ruxin
R
Chen
Yinjian
Y
Chen
Jianqiu
J
Wu
Geping
G
Gong
Zhenyang
Z
Tang
Jiangfeng
J
Li
Genhong
G
Zhu
Chunsheng
C
D000818:Animals; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D006168:Guinea Pigs; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism; D012220:Rhinitis / Q000378:metabolism*
D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide