Biosynthesis of baccharis oxide, a triterpene with a 3,10-oxide bridge in the A-ring.
A new oxidosqualene cyclase (OSC) cDNA was cloned from the roots of Stevia rebaudiana. Functional expression in yeast and spectral analyses of the products established that the obtained OSC yields baccharis oxide as the major product. This is the first identification of an OSC yielding baccharis oxide. This result clearly demonstrated that baccharis oxide, a baccharane triterpene with a unique 3,10-oxide bridge in the A-ring, is a direct product from oxidosqualene by an OSC.
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Masaaki
M
Shibuya
Aya
A
Sagara
Ai
A
Saitoh
Tetsuo
T
Kushiro
Yutaka
Y
Ebizuka
D003500:Cyclization; D004852:Epoxy Compounds / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014315:Triterpenes / Q000737:chemistry* / Q000378:metabolism
D004852:Epoxy Compounds; D014315:Triterpenes; C533357:baccharis oxide