Amelioration of diabetic tubulointerstitial damage in liver-type fatty acid-binding protein transgenic mice.
BACKGROUND
Renoprotection of liver-type fatty acid binding protein (L-FABP) was demonstrated in a streptozotocin (STZ)-induced diabetic mouse model.


METHODS
Established human L-FABP (hL-FABP) transgenic (Tg) mice and wild-type (WT) mice were divided into two groups: diabetic mice were uninephrectomized and injected with STZ; control mice were uninephrectomized and injected with a citrate buffer alone. Although mouse L-FABP was not expressed in WT mice, hL-FABP was expressed in the proximal tubules of the diabetic Tg mice and in the control Tg mice at 8 and 14 weeks after these injections.


RESULTS
The expression of renal hL-FABP increased significantly in diabetic Tg mice compared to control Tg mice. A number of macrophages (F4/80) infiltrating the interstitium, the gene expressions of MCP-1, MCP-3, TGF-beta, Fas, Bax and RAGE were significantly lower in diabetic Tg kidneys compared with diabetic WT kidneys. In the diabetic Tg kidneys, the degree of the tubulointerstitial injury and the deposition of type IV collagen were significantly lower than that of diabetic WT kidneys. The expressions of catalase and glutathione peroxidase-1 were significantly lower in diabetic Tg kidneys compared with diabetic WT kidneys.


CONCLUSIONS
Renal L-FABP ameliorated the tubulointerstitial damage of type 1 diabetic mice.
Department of Anatomy, Division of Medicine, Internal Medicine, St Marianna University School of Medicine, Tokyo, Japan.
Atsuko
A
Kamijo-Ikemori
Takeshi
T
Sugaya
Ayako
A
Sekizuka
Kazuaki
K
Hirata
Kenjiro
K
Kimura
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D003928:Diabetic Nephropathies / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D007684:Kidney Tubules / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018945:Monocyte Chemoattractant Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
D050556:Fatty Acid-Binding Proteins; D018945:Monocyte Chemoattractant Proteins; D012333:RNA, Messenger