An efficient fluorimetric method to measure the viability of intraerythrocytic Plasmodium falciparum.
A range of various assays to measure chemosusceptibility of Plasmodium falciparum have been described in the literature. As the screening of a plethora of compounds for antiplasmodial activity is urgently needed and becomes a constantly increasing routine analysis, a test system has to fulfill the following requirements: sensitivity, reliability, simplicity of performance, high-throughput compatibility, and cost-effectiveness. Here, we describe an assay that fulfills all criteria and in which the fluorescent SYTOX Green dye is introduced to determine growth inhibition of Plasmodia in in vitro cultures.
1Department of Zoophysiology, Zoological Institute, University of Kiel, Olshausenstr. 40, D-24098 Kiel, Germany.
Astrid
A
Evers
Saskia
S
Heppner
Matthias
M
Leippe
Christoph
C
Gelhaus
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002738:Chloroquine / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000191:economics* / Q000379:methods*; D004912:Erythrocytes / Q000469:parasitology*; D005456:Fluorescent Dyes; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009930:Organic Chemicals; D010963:Plasmodium falciparum / Q000187:drug effects / Q000502:physiology*; D013050:Spectrometry, Fluorescence
D000962:Antimalarials; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C402795:SYTOX Green; D002738:Chloroquine; D007770:L-Lactate Dehydrogenase