The rate of false positive sequence matches of peptides profiled by MALDI MS and identified by MS/MS.
In most MALDI peptide profiling cases, sequencing is required to identify peptides of interest, preferentially by using different mass spectrometry techniques. Using identical samples, we determined the number of false positive matches in sequence of peptide identification using different mass spectrometers. This paper demonstrates that the reliability of the identification phase greatly benefits from concerted MS-technologies and determines the influence of mass accuracy, signal-to-noise and statistical score on peptide identification.
Laboratories of Neuro-Oncology/Clinical and Cancer Proteomics, Department of Neurology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
Marcel P
MP
Stoop
Robert-Jan A N
RJ
Lamers
Peter C
PC
Burgers
Peter A E
PA
Sillevis Smitt
Rogier Q
RQ
Hintzen
Theo M
TM
Luider
D000595:Amino Acid Sequence; D001483:Base Sequence; D002555:Cerebrospinal Fluid / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D036103:Nanotechnology; D009862:Online Systems; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
D010446:Peptide Fragments; D010455:Peptides; D014357:Trypsin