Circulating malondialdehyde-modified low-density lipoprotein is strongly associated with very small low-density lipoprotein cholesterol concentrations in healthy men.
BACKGROUND
Despite the importance of oxidized LDL and small LDL particles as atherogenic lipoproteins, the relationship between oxidized LDL and the distributions of size subclasses of lipoproteins is not fully proved. We investigated the relationship of circulating malondialdehyde-modified (MDA)-LDL, an oxidized form of LDL, and lipoprotein subclasses in healthy men.


METHODS
The study group consisted of a total of 170 healthy Japanese men (55+/-9 y). Plasma cholesterol concentrations in major lipoproteins and their subclasses were determined by HPLC with gel permeation columns.


RESULTS
In univariate analysis, body mass index, waist circumference, blood pressure, white blood cell count, C-reactive protein, uric acid, fasting insulin, HOMA-IR, total cholesterol, triglycerides, each VLDL subclass cholesterol, each LDL subclass cholesterol, small HDL cholesterol, and very small HDL cholesterol were positively correlated with MDA-LDL, whereas adiponectin and large HDL cholesterol were inversely correlated with MDA-LDL. In stepwise multiple regression analysis, very small LDL cholesterol, medium VLDL cholesterol, very small HDL cholesterol, small HDL cholesterol, and systolic blood pressure were identified as independent determinants of MDA-LDL (R(2)=0.718, p<0.0001).


CONCLUSIONS
Circulating MDA-LDL concentrations are strongly associated with very small LDL cholesterol concentrations in healthy men. HDL size heterogeneity has a biphasic effect on MDA-LDL.
Department of Cardiology, Chunichi Hospital, Nagoya, Japan.
Ryotaro
R
Takahashi
Akiko
A
Imamura
Mari
M
Yoshikane
Masayuki
M
Suzuki
Xian Wu
XW
Cheng
Yasushi
Y
Numaguchi
Nobuo
N
Ikeda
Toyoaki
T
Murohara
Kenji
K
Okumura
D000368:Aged; D044466:Asian People; D001794:Blood Pressure; D001823:Body Composition; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000032:analysis / Q000378:metabolism; D015243:Cholesterol, VLDL / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006262:Health; D006801:Humans; D007328:Insulin / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000031:analogs & derivatives* / Q000097:blood; D012016:Reference Values; D012044:Regression Analysis; D014280:Triglycerides / Q000097:blood; D014527:Uric Acid / Q000097:blood; D055105:Waist Circumference; D049629:Waist-Hip Ratio
D015243:Cholesterol, VLDL; D007328:Insulin; D008077:Lipoproteins, LDL; D014280:Triglycerides; C502964:malondialdehyde-low density lipoprotein, mouse; D014527:Uric Acid; D008315:Malondialdehyde; D002097:C-Reactive Protein