PDGFRA/NG2 glia generate myelinating oligodendrocytes and piriform projection neurons in adult mice.
Platelet-derived growth factor alpha receptor (PDGFRA)/NG2-expressing glia are distributed throughout the adult CNS. They are descended from oligodendrocyte precursors (OLPs) in the perinatal CNS, but it is not clear whether they continue to generate myelinating oligodendrocytes or other differentiated cells during normal adult life. We followed the fates of adult OLPs in Pdgfra-creER(T2)/Rosa26-YFP double-transgenic mice and found that they generated many myelinating oligodendrocytes during adulthood; >20% of all oligodendrocytes in the adult mouse corpus callosum were generated after 7 weeks of age, raising questions about the function of the late-myelinating axons. OLPs also produced some myelinating cells in the cortex, but the majority of adult-born cortical cells did not appear to myelinate. We found no evidence for astrocyte production in gray or white matter. However, small numbers of projection neurons were generated in the forebrain, especially in the piriform cortex, which is the main target of the olfactory bulb.
Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
Leanne E
LE
Rivers
Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
Kaylene M
KM
Young
Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
Matteo
M
Rizzi
Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
Françoise
F
Jamen
Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
Konstantina
K
Psachoulia
Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
Anna
A
Wade
Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
Nicoletta
N
Kessaris
Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
William D
WD
Richardson
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D053687:Adult Stem Cells / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000941:Antigens / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology* / Q000502:physiology; D004965:Estrogen Antagonists / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D004676:Myelin Basic Protein / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D022661:RNA, Untranslated; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000235:genetics; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000502:physiology*; D055757:SOXE Transcription Factors / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology; D000099216:Chondroitin Sulfate Proteoglycan 4
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D000941:Antigens; D051792:Basic Helix-Loop-Helix Proteins; D001973:Bromodeoxyuridine; D004965:Estrogen Antagonists; D008164:Luminescent Proteins; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D010751:Phosphopyruvate Hydratase; D011506:Proteins; D011509:Proteoglycans; D022661:RNA, Untranslated; D051498:Receptor, Fibroblast Growth Factor, Type 3; D020796:Receptor, Platelet-Derived Growth Factor alpha; D055757:SOXE Transcription Factors; D013629:Tamoxifen; D000099216:Chondroitin Sulfate Proteoglycan 4; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; C512773:Olig2 protein, mouse; C527924:Sox10 protein, mouse