Enhancement of laser-induced fluorescence at 473 nm excitation with subwavelength resonant waveguide gratings.
A dielectric subwavelength resonant waveguide grating was designed and fabricated in order to enhance fluorescence of biomolecules. More than 80 times higher laser-induced fluorescence yield was observed from enhanced green fluorescence protein on the structure when compared to same material on a flat surface.
Karvinen
Petri
P
Department of Physics and Mathematics, University of Joensuu, Joensuu, Finland. petri.karvinen@joensuu.fi
Nuutinen
Tarmo
T
Hyvärinen
Outi
O
Vahimaa
Pasi
P
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007834:Lasers; D008029:Lighting / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*