Fungal transcript pattern during the preinfection stage (12 h) of ectomycorrhiza formed between Pisolithus tinctorius and Castanea sativa roots, identified using cDNA microarrays.
Transcriptional changes in Pisolithus tinctorius leading to ectomycorrhizal formation in P. tinctorius- Castanea sativa were investigated using a 12-h fungal interaction in vitro system. Using a 3107-cDNA clone microarray, 34 unique expressed sequence tags (ESTs) were found to be differentially expressed. These ESTs represent 14 known genes, 5 upregulated and 9 downregulated, and 20 orphan sequences. Some transcripts of upregulated genes (with unknown function) were previously identified in other mycorrhizal Pisolithus spp. associations. ESTs for S-adenosyl-L-homocysteine hydrolase and several orphan sequences were identified in our system. The identified transcript of downregulated genes involved hydrophobins, 5S, 18S, and 28S ribosomal RNA genes, large subunits of ribosomal RNA (mitochondrial gene), and two types of heat shock proteins. This study demonstrates the high complexity of molecular events involved in the preinfection steps and suggests the utilization of different fungal gene repertories before ectomycorrhizal formation. These data constitute a first contribution for the molecular understanding of early signaling events between P. tinctorius and C. sativa roots during ectomycorrhizal formation.
Acioli-Santos
Bartolomeu
B
Laboratório micorrizas, Departamento de Micologia, Universidade Federal de Pernambuco, Recife, Brazil. bartacioli@yahoo.com
Sebastiana
Mónica
M
Pessoa
Fernando
F
Sousa
Lisete
L
Figueiredo
Andreia
A
Fortes
Ana Margarida
AM
Baldé
Aladje
A
Maia
Leonor C
LC
Pais
Maria S
MS
D001487:Basidiomycota / Q000235:genetics* / Q000254:growth & development*; D020224:Expressed Sequence Tags; D029962:Fagaceae / Q000382:microbiology*; D005656:Fungal Proteins / Q000096:biosynthesis; D020869:Gene Expression Profiling; D038821:Mycorrhizae / Q000235:genetics / Q000254:growth & development; D020411:Oligonucleotide Array Sequence Analysis; D018517:Plant Roots / Q000382:microbiology; D012331:RNA, Fungal / Q000096:biosynthesis
D005656:Fungal Proteins; D012331:RNA, Fungal