Molecular cloning and expression patterns of a putative  olfactory diacylglycerol kinase from the noctuid moth  Spodoptera littoralis.
In the aim of the characterization of the molecular actors of insect olfactory transduction, we have cloned the full cDNA encoding a Spodoptera littoralis diacylglycerol kinase (DGK) named SlDGK. In male adults, SlDGK transcript was detected predominantly in the brain and in the olfactory sensilla trichodea located on the antennae. SlDGK expression was first detected at day 3 of the pupal stage, then reached a maximum at the end of this stage and was maintained at this level during the adult period. These data provide the first molecular characterization of a DGK potentially involved in the regulation of signalling pathways responsible for the establishment and/or the functioning of the olfactory system in Lepidoptera.
Chouquet
B
B
UMR 1272, UPMC-INRA-AgroParisTech, Physiologie de l'Insecte: Signalisation et Communication, Université Paris VI, Paris, France.
Bozzolan
F
F
Solvar
M
M
Duportets
L
L
Jacquin-Joly
E
E
Lucas
P
P
Debernard
S
S
D000595:Amino Acid Sequence; D000825:Animal Structures / Q000201:enzymology / Q000648:ultrastructure; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D019852:Diacylglycerol Kinase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008969:Molecular Sequence Data; D009833:Olfactory Pathways / Q000201:enzymology*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera / Q000201:enzymology* / Q000235:genetics* / Q000648:ultrastructure
D018076:DNA, Complementary; D019852:Diacylglycerol Kinase