A membrane-, mediator-, cofactor-less glucose/oxygen biofuel cell.
We report the fabrication and characterisation of a non-compartmentalised, mediator and cofactor free glucose-oxygen biofuel cell based on adsorbed enzymes exhibiting direct bioelectrocatalysis, viz. cellobiose dehydrogenase from Dichomera saubinetii and laccase from Trametes hirsuta as the anodic and cathodic bioelements, respectively, with the following characteristics: an open-circuit voltage of 0.73 V; a maximum power density of 5 microW cm(-2) at 0.5 V of the cell voltage and an estimated half-life of > 38 h in air-saturated 0.1 M citrate-phosphate buffer, pH 4.5 containing 5 mM glucose.
Coman
Vasile
V
Department of Analytical Chemistry, Lund University, 22100 Lund, Sweden.
Vaz-Domínguez
Cristina
C
Ludwig
Roland
R
Harreither
Wolfgang
W
Haltrich
Dietmar
D
De Lacey
Antonio L
AL
Ruzgas
Tautgirdas
T
Gorton
Lo
L
Shleev
Sergey
S
D001203:Ascomycota / Q000201:enzymology; D001674:Bioelectric Energy Sources; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism; D002384:Catalysis; D004566:Electrodes; D004579:Electron Transport; D005947:Glucose / Q000378:metabolism*; D006207:Half-Life; D042845:Laccase / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D020072:Polyporales / Q000201:enzymology
D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D042845:Laccase; D005947:Glucose; D010100:Oxygen