[Preparation and application of the monoclonal antibody against okadaic acid].
An competitive indirect enzyme-linked immunosorbent assay was developed for measurement of okadaic acid (OA), a marine biotoxin associated with red tide. OA was coupled to BSA and OVA by carbodiimide reaction. OA-BSA as immunogen were injected in BALB/c mice. Titres of the antisera against OA were determinated using OA-OVA as coating ligand by ELISA method. The spleen cells of immunized mice were fused with Sp2/O cells. After cloning, four hybridoma cell strains stably produced anti-OA monoclonal antibody were obtained. The monoclonal antibody were produced by the mouse ascites method. The competitive indirect ELISA for okadaic acid in shellfish was established. Under optimal condition, the detection limit of OA was 31.2 ng/ml and the recovery was 87%-112%, with a mean coefficient of variation of 8.1.
National Marine Environmental Monitoring Center, Dalian 116023, China. ryliu@nmemc.gov.cn
Renyan
R
Liu
Bingjun
B
Chen
Yubo
Y
Liang
Daoyan
D
Xu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D057097:Harmful Algal Bloom; D006825:Hybridomas / Q000378:metabolism; D008387:Marine Toxins / Q000032:analysis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019319:Okadaic Acid / Q000032:analysis* / Q000276:immunology*; D057096:Shellfish Poisoning / Q000382:microbiology*
D000911:Antibodies, Monoclonal; D008387:Marine Toxins; D019319:Okadaic Acid