Circulating 11beta-hydroxysteroid dehydrogenase type 1 mRNA and cardiovascular risk factors.
11beta-Hydroxysteroid dehydrogenase type 1 (11beta-HSD1) has been implicated in the pathogenesis of central obesity and metabolic syndrome. It has been shown that tissue-specific mRNA is present in blood, raising the possibility that quantifiable levels of 11beta-HSD1 mRNA may also be present in circulation. The aim of this study was to determine whether a relationship exists between circulating 11beta-HSD1 mRNA levels and various cardiovascular risk factors. Whole blood was collected using PAXgene Blood RNA tubes from 73 subjects. Total RNA was extracted, reverse-transcribed to cDNA, and 11beta-HSD1 mRNA was measured by quantitative real time PCR. The median BMI was 24 (range = 17-36) and the concentration of 11beta-HSD1 mRNA tended to decrease with increasing BMI (r=-0.206; P= 0.08). Multiple linear regression analysis identified BMI as an independent predictor of mRNA levels (beta=-0.412, P= 0.002). Other factors included in the analysis are age (beta= 0.952, P < 0.001), smoking (beta=-0.228, P= 0.027), alcohol intake (beta= 0.479, P < 0.001), and duration of menopause (beta=-1.602, P < 0.001). The results suggest a net decrease in systemic expression of 11beta-HSD1 with increased smoking, BMI, and duration of menopause. Increased systemic expression is seen with increasing age and alcohol intake.
Al Bakir
Maise
M
Department of Chemical Pathology, St. Thomas' Hospital, London, United Kingdom.
Butt
Asif N
AN
Swaminathan
R
R
D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015992:Body Mass Index; D002318:Cardiovascular Diseases / Q000097:blood / Q000201:enzymology / Q000235:genetics; D003348:Cortisone / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D009765:Obesity / Q000097:blood / Q000201:enzymology / Q000235:genetics; D012333:RNA, Messenger / Q000097:blood* / Q000235:genetics; D012044:Regression Analysis; D012307:Risk Factors; D011795:Surveys and Questionnaires
D012333:RNA, Messenger; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D003348:Cortisone; D006854:Hydrocortisone