Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.
The commercial PremiTest Salmonella kit uses a multiplexed DNA typing test aimed at identifying common serovars of Salmonella enterica. It was used in assays over a 9-month period in the Belgian reference laboratory that performs the routine identification of Salmonella strains of animal origin. A blind analysis of 754 strains was conducted in parallel by classical serotyping and the PremiTest assay. Full results were available for 685 strains (90.8%) by serotyping, while the remaining 69 strains were found to be nontypeable due to either a lack of surface antigen expression or autoagglutination properties. When the PremiTest assay (version 4.2) was performed with crude bacterial extracts, it identified 658 strains (87.3%), including most strains found to be nontypeable by serotyping. In contrast, it gave no, wrong, dual, or noninterpretable results for 96 strains, for which 23 were caused by assay failures. When purified DNA instead of crude extracts were tested, the number of strains successfully identified to the serovar level increased to 714 (94.7%), while all assay failures were cleared. Our conclusion is that, in its actual development stage, the application of the investigated kit to purified DNA samples offers a valuable alternative to classical serotyping for laboratories performing the routine identification of Salmonella strains belonging to commonly encountered serovars and isolated from a given geographical area, assuming that the system has been validated beforehand with a significant number of strains originating from that particular area.
Wattiau
Pierre
P
Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, Brussels, Belgium. Pierre.Wattiau@var.fgov.be
Van Hessche
Mieke
M
Schlicker
Christine
C
Vander Veken
Heidi
H
Imberechts
Hein
H
D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D046228:Microarray Analysis; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D019779:Salmonella enterica / Q000145:classification* / Q000302:isolation & purification*; D012703:Serotyping
D004269:DNA, Bacterial