[The mechanism of apoptosis in human U87 glioma cells induced by miR-21 antisense oligonucleotide].
OBJECTIVE
To investigate the mechanism of U87 cell apoptosis induced by inhibiting miR-21 expression.


METHODS
Antisense oligonucleotides of miR-21 were chemically synthesized and transfected into U87 cells. The apoptosis, proliferation, and invasion of the cells were evaluated. The relationship between miR-21 and PTEN or caspase was identified by bioinformatics and Western blot.


RESULTS
Inhibiting miR-21 expression led to U87 cell growth suppression, apoptosis induction, invasion reduction, caspase-3 activity elevation and caspase-9 activation, but did not affect PTEN and caspase-8 expression.


CONCLUSIONS
miR-21 may function as an antiapoptotic miRNA in U87 cells. Inhibiting miR-21 expression could induce U87 cell apoptosis via caspase-9 and 3 activation, but not PTEN activation.
Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P. R. China. sl1012002322@126.com
Lei
L
Shi
Zhihao
Z
Cheng
Junxia
J
Zhang
Rui
R
Li
Yongping
Y
You
Zhen
Z
Fu
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004789:Enzyme Activation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D020320:Oligoribonucleotides, Antisense / Q000235:genetics* / Q000494:pharmacology; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D014162:Transfection
C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D020320:Oligoribonucleotides, Antisense; D051059:PTEN Phosphohydrolase; D020169:Caspases