Suppression of gastric cancer growth by baculovirus vector-mediated transfer of normal epithelial cell specific-1 gene.
OBJECTIVE
To study the inhibitory effect of baculovirus-mediated normal epithelial cell specific-1 (NES1) gene therapy on gastric cancer (GC) in vitro and in vivo.


METHODS
We first constructed recombinant baculovirus vectors and then transfected them into gastric cancer cells (SGC-7901). Efficiency of the baculovirus for gene transfer into SGC-7901 cells and cell growth curves were detected by fluorescence microscopy, Western blot and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in vitro, respectively. The therapeutic effect of this gene therapy on GC was confirmed in xenografted nude mice. Tumor growth was determined by tumor volume, and expression of NES1 in tumor was analyzed by immunohistochemistry.


RESULTS
Baculovirus vectors were successfully transfected into SGC-7901 cells. SGC-7901 cells transfected with the NES1 gene inhibited cell growth. In the Bac-NES1 treated group, tumor growth was significantly reduced with a high level of NES1 expression


CONCLUSIONS
Baculovirus-mediated NES1 gene can be used in gene therapy for GC.
Department of Nuclear Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China. lb10363@rjh.com.cn.
Wei
W
Huang
Xiang-Long
XL
Tian
Yun-Lin
YL
Wu
Jie
J
Zhong
Li-Fen
LF
Yu
Sheng-Ping
SP
Hu
Biao
B
Li
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007610:Kallikreins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D013997:Time Factors; D014161:Transduction, Genetic; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
D049452:Green Fluorescent Proteins; C100936:KLK10 protein, human; D007610:Kallikreins