[Detection of Staphylococcus aureus, Shigella spp., Salmonella spp. in food by multiplex PCR].
OBJECTIVE
To establish a multiplex PCR method for simultaneous detection of Staphylococcus aureus, Shigella spp., Salmonella spp. in food.


METHODS
Staphylococcus aureus was enriched by 7.5% NaCl broth while Shigella spp. and Salmonella spp. were enriched by GN medium . The primers were designed according to the gene nuc of Staphylococcus aureus, the gene ipaH of Shigella spp. and the gene invA of Salmonella spp. The target genes of these pathogens in food were amplified by multiplex PCR, which reaction conditions were optimized specifically.


RESULTS
The multiplex PCR method established in this experment was of high specificity, which detection limit was 1 cfu/ml of Staphylococcus aureus, Shigella spp. and Salmonella spp. when the milk samples contaminated with these pathogens.


CONCLUSIONS
The multiplex PCR method, which was rapid, convenient, and with high sensitivity, could be suitable for rapid detection of Staphylococcus aureus, Shigella spp., Salmonella spp. in food, and could have a great prospect.
College of Food Science and Technology, Huazhong Agriculture University, Wuhan 430070, China.
Bo
B
Li
Fusheng
F
Chen
Xiaohong
X
Wang
Yanchun
Y
Shao
D000818:Animals; D002417:Cattle; D003611:Dairy Products / Q000382:microbiology; D005516:Food Microbiology; D006801:Humans; D008461:Meat Products / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000302:isolation & purification*; D012760:Shigella / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013552:Swine