[Study on glutamate substrate-induced regulation on expression of glutamate transporter GLAST of mouse retinal Müller cells].
OBJECTIVE
To study glutamate substrate-induced regulation on the expression of glutamate transporter L-glutamate/L-aspartate transporter (GLAST) of retinal Müller cells (RMCs) in mice.


METHODS
Activity of GLAST was measured through L-3 H-glutamate uptake detection. The expression of GLAST protein in lysates cells was studied using Western blot.


RESULTS
Glutamate was applied to cultured RMCs at various concentrations for 30 min. L-3 H-glutamate accumulation in RMCs increased as the increase of concentration of substrate glutamate and reached the maximum at 100 micromol/L glutamate. Glutamate treatment of RMCs increased GLAST transporter expression as compared with untreated cultures. Glutamate at 100 micromol/L caused a maximal increase of GLAST protein expression.


CONCLUSIONS
Glutamate substrate increases GLAST activity and up-regulate the expression of GLAST protein.
Xia
Xiao-bo
XB
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008, China. xbxia@yahoo.com
Zhou
Xia
X
Zeng
Qi
Q
D000818:Animals; D002478:Cells, Cultured; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism*; D005786:Gene Expression Regulation; D018698:Glutamic Acid / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D012160:Retina / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
D027341:Excitatory Amino Acid Transporter 1; C493393:Slc1a3 protein, mouse; D018698:Glutamic Acid