Functional expression of the glycine transporter 1 on bullfrog retinal cones.
Using patch clamp techniques, we characterized glycine-induced currents from cones in bullfrog retinal slices. Application of glycine to cone terminals induced an inward current, which was in part suppressed by strychnine. The remaining strychnine-resistant current component, which did not show polarity reversion in a range of -120 mV to +40 mV, was blocked by N[3-(4'-fluorophenyl)-3-(4'-phenylphenoxy)propyl] sarcosine, an antagonist of glycine transporter 1 (GlyT1), but not affected by amoxapine, an inhibitor of glycine transporter 2. Application of sarcosine, an agonist of GlyT1, to cone terminals induced an inward current that was completely suppressed by N[3-(4'-fluorophenyl)-3-(4'-phenylphenoxy)propyl] sarcosine or when external Na in Ringer's was replaced by choline. All these results show for the first time the functional expression of GlyT1 on bullfrog cones.
Zhang
Xin-Jun
XJ
Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China.
Ge
Li-Hao
LH
Liu
Jian
J
Yang
Xiong-Li
XL
D000657:Amoxapine / Q000494:pharmacology; D000818:Animals; D005998:Glycine / Q000494:pharmacology; D018684:Glycine Agents / Q000494:pharmacology; D050487:Glycine Plasma Membrane Transport Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011892:Rana catesbeiana; D017949:Retinal Cone Photoreceptor Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012521:Sarcosine / Q000494:pharmacology; D013331:Strychnine / Q000494:pharmacology
D018684:Glycine Agents; D050487:Glycine Plasma Membrane Transport Proteins; D014179:Neurotransmitter Uptake Inhibitors; D013331:Strychnine; D000657:Amoxapine; D005998:Glycine; D012521:Sarcosine