Crystallographic studies of DNA and RNA.
Our knowledge of nucleic acid structure grew rapidly over the past decade with the determination to high resolution of larger structures of great biological significance. Advances in sample preparation, crystallization techniques, cryocrystallography, access to synchrotron radiation, and crystallographic software continue to accelerate the structure determination of nucleic acids. Crystallographic studies of DNA and RNA molecules share many considerations that we outline here. The application of crystallography to RNA is illustrated with the structure determination of the CUG repeat that is linked to type I myotonic dystrophy.
Mooers
Blaine H M
BH
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, 975 NE 10th Street, BRC 466, Oklahoma City, OK 73104, USA. blaine-mooers@ouhsc.edu
D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D065636:Myotonin-Protein Kinase; D033363:Neutron Diffraction; D009690:Nucleic Acid Conformation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics; D012313:RNA / Q000737:chemistry*
C585517:DMPK protein, human; D012313:RNA; D004247:DNA; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases