[Effect of cedemex on cAMP and cGMP levels of different brain areas in morphine withdrawal rats].
OBJECTIVE
To investigate the effect of Cedemex on cAMP and cGMP contents in different brain regions in morphine withdrawal rats precipitated by naloxone.


METHODS
A physical morphine dependent model of rats was established by subcutaneous injection of morphine in gradually increasing dosage within 7 days. cAMP and cGMP contents of VTA, cortex and hippocampus of the rat brains were determined by radioimmunoassay.


RESULTS
The morphine withdrawal symptoms of rats were relieved significantly by ig Cedemex. Compared with the controls, cAMP content in the region of VTA, cortex and hippocampus of the morphine dependent rats were significantly higher (P < 0.05), while cGMP contents in those regions were significantly lower (P < 0.05). cAMP contents in the area of VTA, cortex and hippocampus of the morphine dependent rats were significantly reduced, while cGMP contents were significantly increased by ig Cedemex.


CONCLUSIONS
Cedemex may significantly attenuate the morphine withdrawal symptoms in rats. The mechanism of this effect may be related to adjusting the contents of cAMP and cGMP in some brain regions.
Department of Pharmacology, Guangxi Medical University, Nanning 530021, China.
Hai-yuan
HY
Xie
Shu
S
Lai
Jian-chun
JC
Huang
Wei-zhe
WZ
Jiang
Song-chao
SC
Guo
Ren-bin
RB
Huang
Phu Kieu
PK
Nguyen
Jin-min
JM
Fan
Yue-gao
YG
Liang
Ting-ping
TP
Chen
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D009020:Morphine / Q000009:adverse effects*; D051381:Rats; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; C540580:cedemex; D009020:Morphine; D000242:Cyclic AMP; D006152:Cyclic GMP