Antioxidant effects of different extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus.
Considering the important role of oxidative stress in the pathogenesis of several neurological diseases, and the growing evidence of the presence of compounds with antioxidant properties in the plant extracts, the aim of the present study was to investigate the antioxidant capacity of three plants used in Brazil to treat neurological disorders: Melissa officinalis, Matricaria recutita and Cymbopogon citratus. The antioxidant effect of phenolic compounds commonly found in plant extracts, namely, quercetin, gallic acid, quercitrin and rutin was also examined for comparative purposes. Cerebral lipid peroxidation (assessed by TBARS) was induced by iron sulfate (10 microM), sodium nitroprusside (5 microM) or 3-nitropropionic acid (2 mM). Free radical scavenger properties and the chemical composition of plant extracts were assessed by 1'-1' Diphenyl-2' picrylhydrazyl (DPPH) method and by Thin Layer Chromatography (TLC), respectively. M. officinalis aqueous extract caused the highest decrease in TBARS production induced by all tested pro-oxidants. In the DPPH assay, M. officinalis presented also the best antioxidant effect, but, in this case, the antioxidant potencies were similar for the aqueous, methanolic and ethanolic extracts. Among the purified compounds, quercetin had the highest antioxidant activity followed by gallic acid, quercitrin and rutin. In this work, we have demonstrated that the plant extracts could protect against oxidative damage induced by various pro-oxidant agents that induce lipid peroxidation by different process. Thus, plant extracts could inhibit the generation of early chemical reactive species that subsequently initiate lipid peroxidation or, alternatively, they could block a common final pathway in the process of polyunsaturated fatty acids peroxidation. Our study indicates that M. officinalis could be considered an effective agent in the prevention of various neurological diseases associated with oxidative stress.
Pereira
Romaiana Picada
RP
Departamento de Química, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Fachinetto
Roselei
R
de Souza Prestes
Alessandro
A
Puntel
Robson Luiz
RL
Santos da Silva
Gloria Narjara
GN
Heinzmann
Berta Maria
BM
Boschetti
Ticiane Krapf
TK
Athayde
Margareth Linde
ML
Bürger
Marilise Escobar
ME
Morel
Ademir Farias
AF
Morsch
Vera Maria
VM
Rocha
João Batista Teixeira
JB
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D031743:Cymbopogon / Q000737:chemistry*; D005296:Ferrous Compounds / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005707:Gallic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D031334:Melissa / Q000737:chemistry*; D009574:Nitro Compounds / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011422:Propionates / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D005296:Ferrous Compounds; D016166:Free Radical Scavengers; D009574:Nitro Compounds; D010636:Phenols; D010936:Plant Extracts; D011422:Propionates; D017392:Thiobarbituric Acid Reactive Substances; D009599:Nitroprusside; C012526:quercitrin; C020748:ferrous sulfate; D012431:Rutin; D005707:Gallic Acid; D011794:Quercetin; C015392:3-nitropropionic acid