[Inhibitory effect of Gracilaria lemaneformis (Bory) Weber Bosse on the co-cultured Scrippsiella trochoidea (Stein) Loeblich III under controlled laboratory conditions].
The inhibitory effects of Grcilaria lemaneiformis on the co-cultured Scrippsiella trochoidea were determined under controlled laboratory conditions, and the possible mechanism was studied. Results showed that: (1) in the separating S. trochoiea-G. lemaneormis co-culture system when the initial cell density of C. lemaneaonis was set at 0.5 g x L(-1), the growth of S. trochoidea was obviously inhibited and its maximum cell density and exponential phase were decreased compared with the control; however, the inhibitory effect was not as strong as that in the direct cell-cell contact co-culture. Result showed that allelopathy basing on the direct cell contact was the most possible reason leading to the observed result; (2) when the initial cell density of G. lemaneiformis was set at 0.2 g'L-' in the direct cell-cell contact co-culture, the intracellular nitrate concentration of S. trochoidea in monoculture system was 1.5 times of that in co-culture. It seemed that G. lemaneiformis could competitively absorb the environmental nitrate and ultimately led to the decrease of the stock of intracellular nitrate of S. trochoidea.
Zhang
Shan-dong
SD
Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. sdzhang@yic.ac.cn
Song
Xiu-xian
XX
Cao
Xi-hua
XH
Yu
Zhi-ming
ZM
D000818:Animals; D018920:Coculture Techniques; D003470:Culture Media; D004141:Dinoflagellida / Q000502:physiology*; D052918:Environmental Restoration and Remediation / Q000379:methods; D044724:Gracilaria / Q000502:physiology*; D014876:Water Pollution / Q000517:prevention & control*
D003470:Culture Media