Oxidative stress disturbs energy metabolism of mitochondria in ethanol-induced gastric mucosa injury.
OBJECTIVE
To study the role of mitochondrial energy disorder in the pathogenesis of ethanol-induced gastric mucosa injury.


METHODS
Wistar rats were used in this study. A gastric mucosal injury model was established by giving the rats alcohol. Gross and microscopic appearance of gastric mucosa and ultrastructure of mitochondria were evaluated. Malondiadehyde (MDA) in gastric mucosa was measured with thiobarbituric acid. Expression of ATP synthase (ATPase) subunits 6 and 8 in mitochondrial DNA (mtDNA) was determined by reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
The gastric mucosal lesion index was correlated with the MDA content in gastric mucosa. As the concentration of ethanol was elevated and the exposure time to ethanol was extended, the content of MDA in gastric mucosa increased and the extent of damage aggravated. The ultrastructure of mitochondria was positively related to the ethanol concentration and exposure time. The expression of mtDNA ATPase subunits 6 and 8 mRNA declined with the increasing MDA content in gastric mucosa after gavage with ethanol.


CONCLUSIONS
Ethanol-induced gastric mucosa injury is related to oxidative stress, which disturbs energy metabolism of mitochondria and plays a critical role in the pathogenesis of ethanol-induced gastric mucosa injury.
Pan
Jin-Shui
JS
Zhongshan Hospital, Xiamen University, China.
He
Shao-Zhen
SZ
Xu
Hong-Zhi
HZ
Zhan
Xiao-Juan
XJ
Yang
Xiao-Ning
XN
Xiao
Hong-Min
HM
Shi
Hua-Xiu
HX
Ren
Jian-Lin
JL
D000818:Animals; D004272:DNA, Mitochondrial / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
D004272:DNA, Mitochondrial; D012333:RNA, Messenger; D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol; D008315:Malondialdehyde; C412204:ATP synthase subunit 6; D025261:Mitochondrial Proton-Translocating ATPases