The prognostic impact of NF-kappaB p105, vimentin, E-cadherin and Par6 expression in epithelial and stromal compartment in non-small-cell lung cancer.
Vimentin, nuclear factor-kappaB (NF-kappaB) p105, fascin, E-cadherin, TGF-beta, Par6 and atypical PKC are molecular markers that play an important role in cell differentiation. Herein, we investigate their prognostic impact in primary non-small-cell carcinoma (NSCLC). Tumour tissue samples from 335 resected patients with stage I-IIIA were used. Tissue microarrays were constructed from duplicate cores of both neoplastic cells and stromal cells and were immunohistochemically evaluated. In univariate analyses, high tumour epithelial cell expressions of NF-kappaB p105 (P=0.02) and E-cadherin (P=0.03) were positive prognostic indicators for disease-specific survival (DSS), whereas high tumour epithelial cell expression of vimentin (P=0.001) was a negative prognostic indicator. High expression of NF-kappaB p105 (P=0.001) and Par6 (P=0.0001) in the stromal compartment correlated with a good prognosis. In multivariate analyses, the tumour epithelial cell expression of NF-kappaB p105 (P=0.0001) and vimentin (P=0.005) and the stromal cell expression of NF-kappaB p105 (P=0.007) and Par6 (P=0.0001) were independent prognostic factors for DSS. High expression of NF-kappaB p105 and low expression of vimentin in tumour epithelial cells are independent predictors of better survival in primary NSCLC. In stromal cells, high expressions of NF-kappaB p105 and Par6 are both favourable independent prognostic indicators.
Institute of Medical Biology, University of Tromso-Norway, Tromso, Norway. samer.al-saad@unn.no
S
S
Al-Saad
K
K
Al-Shibli
T
T
Donnem
M
M
Persson
R M
RM
Bremnes
L-T
LT
Busund
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015820:Cadherins / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry / Q000401:mortality* / Q000473:pathology; D004847:Epithelial Cells / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis; D008175:Lung Neoplasms / Q000737:chemistry / Q000401:mortality* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D052002:NF-kappa B p50 Subunit / Q000032:analysis*; D016016:Proportional Hazards Models; D011493:Protein Kinase C / Q000032:analysis; D017154:Stromal Cells / Q000737:chemistry; D046888:Tissue Array Analysis; D016212:Transforming Growth Factor beta / Q000502:physiology; D014746:Vimentin / Q000032:analysis*; D000099293:Protein Kinase C-lambda
D048868:Adaptor Proteins, Signal Transducing; D015820:Cadherins; D007527:Isoenzymes; D052002:NF-kappa B p50 Subunit; D011493:Protein Kinase C; D016212:Transforming Growth Factor beta; D014746:Vimentin; D000099293:Protein Kinase C-lambda; C499657:PARD6A protein, human