In vitro characterization of embryionic ST14A-cells.
The embryonic striatal temperature sensitive immortalized ST14A-cell line was characterized in vitro by immunocytochemistry when cultured at 33 degrees C and at nonpermissive temperature of 39 degrees C for up to 14 days. At 33 degrees C in DMEM/10% FCS, cells proliferated, were extensively expressing the neural progenitor cell markers nestin and vimentin contrary to neuronal markers. However, when cultured at 39 degrees C the proliferation was delayed and cells began to increase the expression of neuronal markers, followed by a decrease of nestin and vimentin. In serum-free medium the process of neuronal differentiation became more obvious, indicating the potential to use these cells for experimental restorative therapies.
Institute of Anatomy, University of Rostock, Rostock, Germany.
Marine
M
Hovakimyan
Karola
K
Weinreich
Stefan Jean-Pierre
SJ
Haas
Elena
E
Cattaneo
Arndt
A
Rolfs
Andreas
A
Wree
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D003342:Corpus Striatum / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D006816:Huntington Disease / Q000628:therapy; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D055495:Neurogenesis / Q000502:physiology*; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013696:Temperature; D014746:Vimentin / Q000378:metabolism
D015415:Biomarkers; D016895:Culture Media, Serum-Free; D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D014746:Vimentin; C085284:microtubule-associated protein 1B