Apoptosis and cell proliferation: the paradox of salivary glands in Sjögren's disease.
OBJECTIVE
To assess apoptosis and proliferation in salivary glands of patients with primary Sjögren's syndrome.


METHODS
Studies were performed in twenty four minor salivary glands from patients with primary Sjögren's syndrome and an equal number of controls. Apoptosis was studied by immunohistochemistry using monoclonal antibodies anti-Fas FasL and Caspase 3 and apoptotic features by TUNEL. Proliferation was assessed with monoclonal anti-PCNA and anti-Ki67 antibodies.


RESULTS
All salivary glands from Sjögren's display apoptotic molecules along the epithelia of salivary ducts and in a smaller amount in acinar tissue. The presence of Caspase 3 Fas FasL was concordant with the expression of apoptosis by TUNEL. Proliferation markers were encountered in inflammatory emigrant cells but not in ductal epithelia nor in acini. Control biopsies poorly expressed apoptotic or proliferation markers.


CONCLUSIONS
Present data suggests that the ductal epithelial and acinar cells of salivary glands from Sjögren's disease patients exhibit increased apoptosis. Proliferation was mainly observed in infiltrating lymphoid cells. Both events constitute a biological paradox related to the inflammatory process of salivary glands in Sjögren's disease.
Centro de Biología Experimental (CBE), Universidad Autónoma de Zacatecas, Guadalupe, Zacatecas, Mexico. herrerar@cantera.reduaz.mx
Rafael
R
Herrera-Esparza
JuanJosé
J
Bollain-y-Goytia
Claudia
C
Ruvalcaba
MaGuadalupe
M
Ruvalcaba
Deyanira
D
Pacheco-Tovar
Esperanza
E
Avalos-Díaz
D000328:Adult; D017209:Apoptosis; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012469:Salivary Glands / Q000166:cytology / Q000473:pathology*; D012859:Sjogren's Syndrome / Q000473:pathology*