Systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes.
To characterize the impact of gut microbiota on host metabolism, we investigated the multicompartmental metabolic profiles of a conventional mouse strain (C3H/HeJ) (n=5) and its germ-free (GF) equivalent (n=5). We confirm that the microbiome strongly impacts on the metabolism of bile acids through the enterohepatic cycle and gut metabolism (higher levels of phosphocholine and glycine in GF liver and marked higher levels of bile acids in three gut compartments). Furthermore we demonstrate that (1) well-defined metabolic differences exist in all examined compartments between the metabotypes of GF and conventional mice: bacterial co-metabolic products such as hippurate (urine) and 5-aminovalerate (colon epithelium) were found at reduced concentrations, whereas raffinose was only detected in GF colonic profiles. (2) The microbiome also influences kidney homeostasis with elevated levels of key cell volume regulators (betaine, choline, myo-inositol and so on) observed in GF kidneys. (3) Gut microbiota modulate metabotype expression at both local (gut) and global (biofluids, kidney, liver) system levels and hence influence the responses to a variety of dietary modulation and drug exposures relevant to personalized health-care investigations.
Claus
Sandrine P
SP
SORA Division, Department of Biomolecular Medicine, Imperial College London, London, UK.
Tsang
Tsz M
TM
Wang
Yulan
Y
Cloarec
Olivier
O
Skordi
Eleni
E
Martin
François-Pierre
FP
Rezzi
Serge
S
Ross
Alastair
A
Kochhar
Sunil
S
Holmes
Elaine
E
Nicholson
Jeremy K
JK
D021542:Amino Acids, Neutral / Q000032:analysis; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology*; D006626:Hippurates / Q000652:urine; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008660:Metabolism; D054892:Metagenome; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype
D021542:Amino Acids, Neutral; D001647:Bile Acids and Salts; D006626:Hippurates; C013809:5-aminovaleric acid; C030514:hippuric acid