Metabolic urinary profiling of alcohol hepatotoxicity and intervention effects of Yin Chen Hao Tang in rats using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry.
This paper was designed to study metabonomic characters of the hepatotoxicity induced by alcohol and the intervention effects of Yin Chen Hao Tang (YCHT), a classic traditional Chinese medicine formula for treatment of jaundice and liver disorders in China. Urinary samples from control, alcohol- and YCHT-treated rats were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-QTOF-MS) in positive ionization mode. The total ion chromatograms obtained from the control, alcohol- and YCHT-treated rats were easily distinguishable using a multivariate statistical analysis method such as the principal components analysis (PCA). The greatest difference in metabolic profiling was observed from alcohol-treated rats compared with the control and YCHT-treated rats. The positive ions m/z 664.3126 (9.00 min) was elevated in urine of alcohol-treated rats, whereas, ions m/z 155.3547 (10.96 min) and 708.2932 (9.01 min) were at a lower concentration compared with that in urine of control rats, however, these ions did not indicate a statistical difference between control rats and YCHT-treated rats. The ion m/z 664.3126 was found to correspond to ceramide (d18:1/25:0), providing further support for an involvement of the sphingomyelin signaling pathway in alcohol hepatotoxicity and the intervention effects of YCHT.
Wang
Xijun
X
Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, No. 24 Heping Road, Harbin 150040, China. wxj@hljucm.net
Lv
Haitao
H
Sun
Hui
H
Liu
Lian
L
Yang
Bo
B
Sun
Wenjun
W
Wang
Ping
P
Zhou
Dixin
D
Zhao
Li
L
Dou
Shengshan
S
Zhang
Guangmei
G
Cao
Hongxin
H
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D064420:Drug-Related Side Effects and Adverse Reactions; D004365:Drugs, Chinese Herbal / Q000378:metabolism*; D000431:Ethanol / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D008297:Male; D008516:Medicine, Chinese Traditional / Q000379:methods*; D055442:Metabolome; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D016482:Urinalysis / Q000379:methods; D014551:Urinary Tract / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D000431:Ethanol