Binding of two transcriptional factors, Xyr1 and ACEI, in the promoter region of cellulase cbh1 gene.
Xyr1 (xylanase regulator 1) and ACEI are two transcriptional factors that regulate xylanases and cellulases formation in Trichoderma reesei. To investigate the transcriptional regulation of cellulases, DNA binding domains of both Xyr1 and ACEI in T. reesei were expressed from E. coli. Electrophoresis mobility shift assays showed that both formed complexes with the cbh1 promoter fragment (-304 to -18), suggesting that Xyr1 and ACEI may regulate cbh1 expression by bindings to the corresponding sites in the cbh1 promoter.
Ling
Min
M
College of Life Science and Technology, Guangxi University, Nanning, 530004, People's Republic of China.
Qin
Yongling
Y
Li
Nan
N
Liang
Zhiqun
Z
D001665:Binding Sites; D002480:Cellulase / Q000235:genetics*; D043364:Endo-1,4-beta Xylanases / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics*; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
C432418:ACEII protein, T. reesei; D015534:Trans-Activators; D014157:Transcription Factors; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases