Use of solid phase microextraction (SPME) for profiling the volatile metabolites produced by Glomerella cingulata.
The profile of volatile organic compounds (VOCs) released from Glomerella cingulata using solid phase microextraction (SPME) with different fibers, Polydimethylsiloxane (PDMS), Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), Carboxen/Polydimethylsiloxane (CAR/PDMS) and Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), was investigated. C4-C6 aliphatic alcohols were the predominant fraction of VOCs isolated by CAR/PDMS fiber. Sesquiterpene hydrocarbons represented 20.3% of VOCs isolated by PDMS fiber. During the growth phase, Ochracin was produced in the large majority of VOCs. 3-Methylbutanol and phenylethyl alcohol were found in the log phase of it. Alcohols were found in cultures of higher age, while sesquiterpenes were found to be characteristic of initial growth stage of G. cingulata.
Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, Higashiosaka-shi, Osaka, Japan. miyazawa@apch.kindai.ac.jp
Mitsuo
M
Miyazawa
Minako
M
Kimura
Yoshito
Y
Yabe
Daisuke
D
Tsukamoto
Masaya
M
Sakamoto
Isao
I
Horibe
Yoshiharu
Y
Okuno
D020081:Phyllachorales / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D052617:Solid Phase Microextraction; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism
D055549:Volatile Organic Compounds