Thin frozen film method for visualization of storage proteins in mature rice grains.
There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.
Saito
Yuhi
Y
Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.
Nakatsuka
Nobuaki
N
Shigemitsu
Takanari
T
Tanaka
Kunisuke
K
Morita
Shigeto
S
Satoh
Shigeru
S
Masumura
Takehiro
T
D005629:Frozen Sections / Q000379:methods*; D012275:Oryza / Q000254:growth & development; D010940:Plant Proteins
D010940:Plant Proteins