Cloning and functional analysis of wheat V-H+-ATPase subunit genes.
The root microsomal proteomes of salt-tolerant and salt-sensitive wheat lines under salt stress were analyzed by two-dimensional electrophoresis and mass spectrum. A wheat V-H(+)-ATPase E subunit protein was obtained whose expression was enhanced by salt stress. In silicon cloning identified the full-length cDNA sequences of nine subunits and partial cDNA sequences of two subunits of wheat V-H(+)-ATPase. The expression profiles of these V-H(+)-ATPase subunits in roots and leaves of both salt-tolerant and salt-sensitive wheat lines under salt and abscisic acid (ABA) stress were analyzed. The results indicate that the coordinated enhancement of the expression of V-H(+)-ATPase subunits under salt and ABA stress is an important factor determining improved salt tolerance in wheat. The expression of these subunits was tissue-specific. Overexpression of the E subunit by transgenic Arabidopsis thaliana was able to enhance seed germination, root growth and adult seedling growth under salt stress.
College of Life Science, Hebei Normal University, Shijiazhuang 050016, People's Republic of China.
Qian
Q
Zhao
Yan-Jun
YJ
Zhao
Bao-Cun
BC
Zhao
Rong-Chao
RC
Ge
Ming
M
Li
Yin-Zhu
YZ
Shen
Zhan-Jing
ZJ
Huang
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D017343:Genes, Plant; D006180:Proton-Translocating ATPases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
D017931:DNA Primers; D006180:Proton-Translocating ATPases