Insulin-like growth factor 1 treatment via hydrogels rescues cochlear hair cells from ischemic injury.
This study was designed to investigate the protective effects of recombinant human insulin-like growth factor 1 (rhIGF1), applied locally via a hydrogel, against ischemic damage of the cochleae in gerbils. A hydrogel was immersed in rhIGF1 or saline and was applied on the round window membrane 30 min after the ischemia. Local rhIGF1 treatment significantly reduced the elevation of auditory brain responses thresholds at a frequency of 8 kHz on days 1, 4, and 7 after ischemia. A histological analysis revealed increased survival of inner hair cells in the animals treated with rhIGF1 via the hydrogel 7 days after ischemia. These findings showed that local rhIGF1 application using a hydrogel has the potential to protect the cochleae from ischemic injury.
Fujiwara
Takashi
T
Department of Otolaryngology, Ehime University Graduate School of Medicine, Shitsukawa, Ehime, Japan.
Hato
Naohito
N
Nakagawa
Takayuki
T
Tabata
Yasuhiko
Y
Yoshida
Tadashi
T
Komobuchi
Hayato
H
Takeda
Shoichiro
S
Hyodo
Jun
J
Hakuba
Nobuhiro
N
Gyo
Kiyofumi
K
D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects; D003051:Cochlea / Q000098:blood supply / Q000473:pathology; D015834:Cochlear Diseases / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D005849:Gerbillinae; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D020100:Hydrogels; D007267:Injections / Q000379:methods; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000627:therapeutic use*; D007511:Ischemia / Q000150:complications* / Q000473:pathology; D008026:Ligation / Q000379:methods; D008297:Male; D012405:Round Window, Ear / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D014711:Vertebral Artery / Q000503:physiopathology
D020100:Hydrogels; D007334:Insulin-Like Growth Factor I