The cell growth suppressor, mir-126, targets IRS-1.
miRNAs are a family of approximately 22-nuleotide-long noncoding RNAs involved in the formation and progress of tumors. Since traditional methods for the detection of miRNAs expression have many disadvantages, we developed a simple method called polyA RT PCR. With this method, we detected a series of miRNAs and found that mir-126 is one of the miRNAs underexpressed in breast cancer cells. Flow cytometry analysis showed that mir-126 inhibited cell cycle progression from G1/G0 to S. Further studies revealed that mir-126 targeted IRS-1 at the translation level. Knocking down of IRS-1 suppresses cell growth in HEK293 and breast cancer cell MCF-7, which recapitulates the effects of mir-126. In conclusion, we developed a simple method for high-throughput screening of miRNAs and found that mir-126, a cell growth suppressor, targets IRS-1.
Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical College, Fudan University, Shanghai, China.
Jin
J
Zhang
Ying-ying
YY
Du
Yi-feng
YF
Lin
Ya-ting
YT
Chen
Lu
L
Yang
Hui-jun
HJ
Wang
Duan
D
Ma
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016193:G1 Phase / Q000235:genetics; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics; D016192:Resting Phase, Cell Cycle / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016196:S Phase / Q000235:genetics
D055504:Insulin Receptor Substrate Proteins; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering