Expression of fascin-1, an actin-bundling protein, in migrating hepatoblasts during rat liver development.
Fascin-1 is an actin-bundling protein localized at the core actin bundles within microvillar projections and filopodial extensions in migrating cells. It is expressed at a low level in normal epithelial cells, but at a high level in tumor cells, indicating its importance in the invasion and motility of tumor cells. In addition, fascin-1 is expressed in human and murine embryos, occurring at high levels especially in developing nervous tissues. In this study, we have investigated the expression patterns of fascin-1 immunohistochemically during the early stages of rat hepatogenesis. A high expression of fascin-1 was detected in the liver bud and hepatoblasts at embryonic day (ED) 10.5, ED11.5, and ED12.5. Expression fell by ED13.5 and was not detectable at ED14.5. These observations demonstrate that the expression of fascin-1 is correlated with the migration activity of hepatoblasts during the early stages of liver development in rats.
Department of Pathology, School of Medicine, Kochi University, Oko-cho, Nankoku, Kochi, 783-8505, Japan. jm-hayashiy@kochi-u.ac.jp
Yoshihiro
Y
Hayashi
Katsumi
K
Toda
Toshiji
T
Saibara
Sumika
S
Okamoto
Makoto
M
Osanai
Hideaki
H
Enzan
Gang-Hong
GH
Lee
D000199:Actins / Q000378:metabolism; D000818:Animals; D002465:Cell Movement; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D047108:Embryonic Development; D008099:Liver / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure*; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar
D000199:Actins; C535054:Fscn1 protein, rat; D008840:Microfilament Proteins