Interplay of RNA Pol IV and ROS1 during post-embryonic 5S rDNA chromatin remodeling.
We have investigated the chromatin structure of 5S rDNA, a heterochromatic pericentromeric tandemly repeated family, at 2, 3, 4 and 5 days post-germination. Our results revealed a large-scale reorganization of 5S rDNA chromatin that occurs during the first days of development. Unexpectedly, there is a decondensation followed by a 're'condensation of 5S rDNA chromatin, to obtain almost mature nuclei 5 d post-germination. The reorganization of 5S rDNA chromatin is accompanied by a rapid and active demethylation of 5S rDNA mediated by the ROS1 (repressor of silencing 1) demethylase, whereas the plant-specific RNA polymerase IV (Pol IV) is essential to the 5S chromatin 're'condensation. In conclusion, Pol IV and ROS1 collaborate to unlock the 5S rDNA chromatin inherited from the seed, and establish adult features.
Douet
Julien
J
CNRS, UMR 6247 GReD, Clermont Université, INSERM, 24 Avenue des Landais, 63177 Aubière Cedex, France.
Blanchard
Bertrand
B
Cuvillier
Claudine
C
Tourmente
Sylvette
S
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D019175:DNA Methylation; D018744:DNA, Plant / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D018525:Germination; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D004275:DNA, Ribosomal; D009687:Nuclear Proteins; D012341:RNA, Ribosomal, 5S; C471271:ROS1 protein, Arabidopsis; C499181:RNA polymerase IV, Arabidopsis; D012321:DNA-Directed RNA Polymerases