Distribution of Crenarchaeota representatives in terrestrial hot springs of Russia and Iceland.
Culture-independent (PCR with Crenarchaeota-specific primers and subsequent denaturing gradient gel electrophoresis) and culture-dependent approaches were used to study the diversity of Crenarchaeota in terrestrial hot springs of the Kamchatka Peninsula and the Lake Baikal region (Russia) and of Iceland. Among the phylotypes detected there were relatives of both cultured (mainly hyperthermophilic) and uncultured Crenarchaeota. It was found that there is a large and diverse group of uncultured Crenarchaeota that inhabit terrestrial hot springs with moderate temperatures (55 to 70 degrees C). Two of the lineages of this group were given phenotypic characterization, one as a result of cultivation in an enrichment culture and another one after isolation of a pure culture, "Fervidococcus fontis," which proved to be a moderately thermophilic, neutrophilic (optimum pH of 6.0 to 7.5), anaerobic organotroph.
Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-letiya Oktyabrya, 7/2, 117312 Moscow, Russia. annprv@gmail.com
Anna A
AA
Perevalova
Tatiana V
TV
Kolganova
Nils-Kåre
NK
Birkeland
Christa
C
Schleper
Elizaveta A
EA
Bonch-Osmolovskaya
Alexander V
AV
Lebedinsky
D016000:Cluster Analysis; D019615:Crenarchaeota / Q000145:classification* / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D045482:Hot Springs / Q000382:microbiology*; D007055:Iceland; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012426:Russia; D017422:Sequence Analysis, DNA
D019641:DNA, Archaeal