[Experimental study on effect and mechanism of soybean protease inhibitor on endotoxin-induced acute lung injury].
OBJECTIVE
To study the protective effect of soybean protease inhibitor on LPS-induced lung injury in rats.


METHODS
Fifty male SD rats were randomly divided in five groups, 10 rats in each group as sham-operation group, model control group, positive medicine group, and high, moderate SBTI groups. Except the sham-group, other groups were induced by intratracheal instillation of LPS with a dose of 6 mg x kg(-1). All rats were given drug throughout intraperitoneal injection except the model controlled group, the positive medicine group was given PMSF with a dose of 50 mg x kg(-1), the high dose group of SBTI was given SBTI with a dose of 100 mg x kg(-1), a dose of the moderate group is 50 mg x kg(-1). We examined all rats in seven days. Index exam: cell quantity, activity of neutrophilic granulocyte released elastic protease proteins in BALF, histopathological examination and so on.


RESULTS
Soybean protease inhibitor can level down the level of total protein, cell quantity, PMN percent, activity of neutrophilic granulocyte in BALF. SBTI level down the content of NF-kappa B in nucleoprotein, while increase the content of I kappa B alpha in plasmoprotein.


CONCLUSIONS
SBTI is useful in protecting experimental pulmonary injury induced by LPS in rats.
Wei
Yan-fei
YF
Lab of Cell & Biochemistry of Beijing University of Chinese medicine, Beijing 100029, China.
Wang
Ji-feng
JF
Niu
Jian-zhao
JZ
You
Dong-ge
DG
D055371:Acute Lung Injury / Q000139:chemically induced* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D000818:Animals; D004731:Endotoxins / Q000633:toxicity*; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020672:I-kappa B Proteins / Q000378:metabolism; D008297:Male; D000072000:NF-KappaB Inhibitor alpha; D051381:Rats; D013025:Glycine max / Q000737:chemistry*; D051996:Transcription Factor RelA / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000494:pharmacology*
D004731:Endotoxins; D020672:I-kappa B Proteins; C000606206:Nfkbia protein, rat; D051996:Transcription Factor RelA; D014361:Trypsin Inhibitors; D000072000:NF-KappaB Inhibitor alpha