Heparin binding haemagglutin as potential diagnostic marker of Mycobacterium bovis.
In this study, we characterized the humoral responses in cattle of Sardinia. The animals were divided into three groups: 1) 28 cattle infected with Mycobacterium bovis; 2) 48 cattle from herds in which foci of infection was notified; 3) 50 cattle from herds that were TB-free. Levels of IgG antibody were measured against the following antigens of M. tuberculosis: Heparin-Binding-Haemagglutin (HBHA), Ag85B, PPE44, and PE_PGRS33 to investigate their potential to diagnose TB in animals. Our results indicated that HBHA is a potential candidate for the development of a serological assay for rapid diagnosis of cattle infected with M. bovis.
Molicotti
Paola
P
Department of Biomedical Sciences, University of Sassari, Italy.
Bua
Alessandra
A
Ortu
Silvia
S
Ladu
Maura C
MC
Delogu
Giovanni
G
Mura
Antonio
A
Sechi
Leonardo A
LA
Fadda
Giovanni
G
Zanetti
Stefania
S
D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002417:Cattle; D037102:Lectins / Q000097:blood* / Q000276:immunology; D025202:Molecular Diagnostic Techniques; D009163:Mycobacterium bovis / Q000276:immunology / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000382:microbiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D037102:Lectins; C104578:heparin-binding hemagglutinin