Specific detection of Xanthomonas oryzae pv. oryzicola in infected rice plant by use of PCR assay targeting a membrane fusion protein gene.
Successful control of Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak, requires a specific and reliable diagnostic tool. A pathovar-specific PCR assay was developed for the rapid and accurate detection of the plant pathogenic bacterium Xanthomonas oryzae pv. oryzicola in diseased plant. Based on differences in a membrane fusion protein gene of Xanthomonas oryzae pv. oryzicola and other microorganisms, which was generated from NCBI (http://www.ncbi.nlm.nih.gov/) and CMR (http://cmr.tigr.org/) BLAST searches, one pair of pathovar-specific primers, XOCMF/XOCMR, was synthesized. Primers XOCMF and XOCMR from a membrane fusion protein gene were used to amplify a 488-bp DNA fragment. The PCR product was only produced from 4 isolates of Xanthomonas oryzae pv. oryzicola among 37 isolates of other pathovars and species of Xanthomonas, Pectobacterium, Pseudomonas, Burkholderia, Escherichia coli, and Fusarium oxysporum f.sp. dianthi. The results suggested that the assay detected the pathogen more rapidly and accurately than standard isolation methods.
Kang
Man Jung
MJ
National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea.
Shim
Jae Kyung
JK
Cho
Min Seok
MS
Seol
Young Joo
YJ
Hahn
Jang Ho
JH
Hwang
Duk Ju
DJ
Park
Dong Suk
DS
D001426:Bacterial Proteins / Q000235:genetics; D030541:Databases, Genetic; D005798:Genes, Bacterial; D050576:Membrane Fusion Proteins / Q000235:genetics*; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000382:microbiology; D014974:Xanthomonas / Q000235:genetics* / Q000302:isolation & purification*
D001426:Bacterial Proteins; D050576:Membrane Fusion Proteins