Expression, purification and characterization of endo-type chitosanase of Aspergillus sp. CJ22-326 from Escherichia coli.
An endo-chitosanase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an endo-chitosanase activity during viscosimetric assay and TLC analysis. The enzyme had higher chitosanolytic activity than previously reported fungal chitosanases.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122 Jiangsu, PR China.
Songlin
S
Li
Liang
L
Chen
Chen
C
Wang
Wenshui
W
Xia
D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D001232:Aspergillus fumigatus / Q000201:enzymology / Q000235:genetics; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology*; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014783:Viscosity
D005656:Fungal Proteins; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019868:chitosanase