A linear ion trap mass spectrometer with versatile control and data acquisition for ion/ion reactions.
A linear ion trap (LIT) with electrospray ionization (ESI) for top-down protein analysis has been constructed. An independent atmospheric sampling glow discharge ionization (ASGDI) source produces reagent ions for ion/ion reactions. The device is also meant to enable a wide variety of ion/ion reaction studies. To reduce the instrument's complexity and make it available for wide dissemination, only a few simple electronics components were custom built. The instrument functions as both a reaction vessel for gas-phase ion/ion reactions and a mass spectrometer using mass-selective axial ejection. Initial results demonstrate trapping efficiency of 70% to 90% and the ability to perform proton transfer reactions on intact protein ions, including dual polarity storage reactions, transmission mode reactions, and ion parking.
Department of Chemistry, Iowa State University, Ames, Iowa, USA.
Matthew W
MW
Soyk
Qin
Q
Zhao
R S
RS
Houk
Ethan R
ER
Badman
D045304:Cytochromes c / Q000737:chemistry; D004581:Electronics / Q000295:instrumentation; D004867:Equipment Design; D007477:Ions; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D014357:Trypsin / Q000737:chemistry; D025801:Ubiquitin / Q000737:chemistry
D007477:Ions; D011506:Proteins; D025801:Ubiquitin; D045304:Cytochromes c; D014357:Trypsin