Integrated multiple multi-photon imaging and Raman spectroscopy for characterizing structure-constituent correlation of tissues.
Histopathological imaging of tissues often requires extensive sample preparation including fixation and staining in order to highlight characteristic alterations associated with diseases. Herein, we report an integrated spectro-microscopy approach based on a combination of multi-modal multi-photon imaging and Raman micro-spectroscopy and demonstrate label-free characterization of the structure-constituent correlation of porcine skin. The multi-modal imaging allows the visualization of dermatological features whereas Raman micro-spectroscopy enables the identification of their 'molecular fingerprints'. By obtaining both structural and molecular-level information of tissue constituents, this integrated approach can offer new insight into the patho-physiological status of tissues.
Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, Hsinchu, Taiwan. ianliau@mail.nctu.edu.tw
Jhen-Wei
JW
Jhan
Wei-Tien
WT
Chang
Hung-Che
HC
Chen
Ming-Fang
MF
Wu
Yu-Tsung
YT
Lee
Chung-Hsuan
CH
Chen
Ian
I
Liau
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods