Preparation of preformed porous PLGA microparticles and antisense oligonucleotides loading.
The objective of this study was to load preformed highly porous microparticles with drug. The microparticles were prepared by a modified multiple emulsion (w/o/w) solvent evaporation method with the addition of pore formers (NaCl into the internal aqueous phase or of glycerol monooleate to the poly(lactide-co-glycolide) (PLGA) polymer phase). The drug-free solidified microparticles were then washed with either water (for NaCl) or hexane (for glycerol monooleate) to extract the pore formers. The drug was then loaded into the preformed porous microparticles by incubation in aqueous drug solutions followed by air- or freeze-drying. The drug was strongly bound to the polymeric surface with air-dried microparticles. A biphasic drug release with an initial rapid release phase (burst effect) was followed by a slower release up to several weeks. The initial burst was dependent on the drug loading and could be significantly reduced by wet (non-aqueous) temperature curing.
College of Pharmacy, Freie Universität Berlin, Berlin, Germany. abidra@hotmail.com
Abid Riaz
AR
Ahmed
Roland
R
Bodmeier
D002626:Chemistry, Pharmaceutical; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D004655:Emulsions; D005612:Freeze Drying; D005989:Glycerides / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D008863:Microspheres; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012965:Sodium Chloride / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013696:Temperature
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004655:Emulsions; D005989:Glycerides; D016376:Oligonucleotides, Antisense; D011108:Polymers; D012997:Solvents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D012965:Sodium Chloride; C005953:monoolein