[Overexpression of core binding factor alpha1 enhancing osteoblastic differentiation in adipose-derived stem cells].
OBJECTIVE
To determine whether mouse core binding factor alpha1 (Cbfalpha1) overexpression enhancing osteoblastic differentiation in adipose-derived stem cells (ADSCs).


METHODS
ADSCs were harvested from SD rats and transduced with the recombinant adenovirus carrying Cbfalpha1 gene. Untransduced cells and cells transduced with adenovirus carrying the enhanced green fluorescence protein (Ad-EGFP) gene served as controls. Cbfalpha1 expression was assessed by immunofluorescence. Alkaline phosphatase (ALP) activity was assayed in 7 and 10 d.


RESULTS
Overexpression of Runx2 inhibited adipogenesis, as demonstrated by suppression of LPL expression. Moreover, ADSCs transduced with Ad-Runx2 underwent rapid and marked osteoblast differentiation as determined by osteoblastic gene expression, alkaline phosphatase activity.


CONCLUSIONS
Cbfalpha1 overexpression enhances osteoblastic differentiation in adipose-derived stem cells.
Institute of Sports Medicine, the Third China Hospital of Peking University, Beijing 100083, China.
Xin
X
Zhang
Min
M
Yang
Lin
L
Lin
Ying-fang
YF
Ao
D000256:Adenoviridae / Q000235:genetics; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D008297:Male; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014162:Transfection
D050659:Core Binding Factor Alpha 1 Subunit