Production and accumulation of xylooligosaccharides with long chains by growing culture and xylanase of a mutant strain of Bacillus pumilus X-6-19.
Bacillus pumilus X-6-9 isolated from soil and subsequently identified, produced xylooligosaccharides with long chains from xylan and accumulated them in the culture. By improving the culture conditions and mutating the bacterium, a 3.2-fold increase in the production of the xylooligosaccharides was established, when compared to the original culture conditions of B. pumilus X-6-19. The addition of D-glucose to the culture of the mutant strain U-3 of B. pumilus X-6-9 repressed the synthesis of beta-xylosidase, but not xylanase. Thus, it was revealed that strain U-3 was a good organism for the production and accumulation of xylooligosaccharides with long chains from xylan by a microbial culture. Xylanase produced by strain U-3 was purified to homogeneity and characterized. The hydrolyzates generated by the purified xylanase contained xylobiose, xylotriose, xylotetraose, and xylopentaose, but not xylose.
Yuan
Qingzhu
Q
Division of Life Science, Graduate School of Science and Technology, Kobe University, Rokko, Kobe 657-8501, Japan.
Adachi
Tsuyoshi
T
Takenaka
Shinji
S
Murakami
Shuichiro
S
Tanaka
Machiko
M
Aoki
Kenji
K
D001407:Bacillus / Q000235:genetics* / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D009154:Mutation; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012988:Soil Microbiology
D009844:Oligosaccharides; D011994:Recombinant Proteins; D043364:Endo-1,4-beta Xylanases; D005947:Glucose