Propagation of a transmissible cytotoxic activity on cultures of human peripheral blood lymphocytes.
Positive results were attained when human peripheral blood lymphocytes (PBLs) were investigated for their ability to propagate a transmissible cytotoxic activity (TCA) isolated on VERO cell cultures from a sample of cerebrospinal fluid (CSF) drawn from a woman with ischemic brain injury. In consideration of this finding it can be assumed that "in vivo" blood lymphocytes contributed to give rise to the TCA detected "in vitro" in the CSF inoculum.
Centre for Diagnosis of Viral Diseases, Department of Laboratory Medicine, University Hospital of Modena and Reggio Emilia, Modena, Italy. marinella.portolani@unimore.it
Marinella
M
Portolani
Francesca
F
Beretti
Anna M
AM
Bartoletti
Paola
P
Pietrosemoli
Stefano
S
Salvioli
Claudio
C
Franceschi
Paola
P
Sena
Anto
A
De Pol
D000818:Animals; D017209:Apoptosis; D018929:Cell Culture Techniques; D002555:Cerebrospinal Fluid / Q000166:cytology; D002522:Chlorocebus aethiops; D003601:Cytotoxicity Tests, Immunologic; D003603:Cytotoxins / Q000134:cerebrospinal fluid / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000097:blood / Q000166:cytology / Q000378:metabolism*; D014709:Vero Cells
D003603:Cytotoxins