MR tracking of iron-labeled glass radioembolization microspheres during transcatheter delivery to rabbit VX2 liver tumors: feasibility study.
OBJECTIVE
To prospectively test the hypothesis that iron labeling of radioembolization microspheres permits their visualization by using magnetic resonance (MR) imaging for in vivo tracking during transcatheter delivery to liver tumors.


METHODS
All experiments were approved by the Institutional Animal Care and Use Committee. Phantom studies were performed to quantify microsphere relaxivity and volume susceptibility properties and compare image contrast patterns resulting from aggregate deposition of unlabeled and iron-labeled microspheres. In seven rabbits in which nine VX2 liver tumors were implanted, T2*-weighted gradient-echo (GRE) MR images with negative image contrast (NC), white-marker (WM) GRE images with positive image contrast (PC), and on-resonance water-suppression turbo spin-echo (SE) images with PC were obtained before and after catheter-directed administration of microspheres into the hepatic artery. During each injection, serial GRE acquisitions were performed for real-time visualization of microsphere delivery. Contrast-to-noise ratios (CNRs) were measured between regions of microsphere accumulation and regions of normal liver parenchyma that demonstrated no apparent microsphere accumulation. Pre- and postinjection CNR measurements at identical spatial positions were compared by using paired t test (alpha = .05).


RESULTS
Conventional microspheres did not produce detectable image contrast in phantoms. Iron-labeled microspheres produced susceptibility-induced dipole patterns with spatial extent of image contrast increasing with increasing microsphere dose. Real-time image series depicted both preferential delivery to tumor tissues and nontargeted delivery to adjacent organs. T2*-weighted GRE, WM GRE, and on-resonance water-suppression turbo SE each permitted in vivo visualization of the microsphere deposition, with postinjection CNR values (mean, 14.29 +/- 3.98 [standard deviation], 1.87 +/- 0.93, and 19.30 +/- 8.72, respectively) significantly greater than corresponding preinjection CNR values (mean, 2.02 +/- 4.65, 0.02 +/- 0.27, 0.85 +/- 2.65, respectively) (P < .05).


CONCLUSIONS
Microsphere tracking during radioembolization may permit real-time verification of delivery and detection of extrahepatic shunting.
Department of Radiology, Northwestern University, 448 E Ontario St, Suite 700, Chicago, IL 60611, USA.
Twinkle
T
Gupta
Sumeet
S
Virmani
Tod M
TM
Neidt
Barbara
B
Szolc-Kowalska
Kent T
KT
Sato
Robert K
RK
Ryu
Robert J
RJ
Lewandowski
Vanessa L
VL
Gates
Gayle E
GE
Woloschak
Riad
R
Salem
Reed A
RA
Omary
Andrew C
AC
Larson
D000818:Animals; D002404:Catheterization; D004617:Embolism; D005240:Feasibility Studies; D005898:Glass; D007501:Iron; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000532:radiotherapy*; D008279:Magnetic Resonance Imaging; D008863:Microspheres; D019047:Phantoms, Imaging; D011817:Rabbits; D015021:Yttrium Radioisotopes / Q000627:therapeutic use
D015021:Yttrium Radioisotopes; D007501:Iron