Self-assembling diblock copolymers of poly[N-(2-hydroxypropyl)methacrylamide] and a beta-sheet peptide.
The self-assembly of hybrid diblock copolymers composed of poly(HPMA) and beta-sheet peptide P11 (CH(3)CO-QQRFQWQFEQQ-NH(2)) blocks was investigated. Copolymers were synthesized via thiol-maleimide coupling reaction, by conjugation of semitelechelic poly(HPMA)-SH with maleimide-modified beta-sheet peptide. As expected, CD and CR binding studies showed that the peptide block imposed its beta-sheet structural arrangement on the structure of diblock copolymers. TEM and AFM proved that peptide and these copolymers had the ability to self-assemble into fibrils.
Department of Bioengineering, University of Utah, Salt Lake City, Utah 84112, USA.
Larisa Cristina
LC
Radu
Jiyuan
J
Yang
Jindrich
J
Kopecek
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D008689:Methacrylates / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics; D011108:Polymers / Q000737:chemistry; D017433:Protein Structure, Secondary
D008689:Methacrylates; D010455:Peptides; D011108:Polymers; C032802:hydroxypropyl methacrylate