Functionalization of multi-walled carbon nanotubes and their application for selective isolation of acidic proteins.
A micro-column packed with PDDA-wrapped MWNTs in sequential injection system facilitates selective sorption of acidic protein species. Proteins adsorbed onto the PDDA-MWNT composites are afterwards collected by elution with a citrate buffer as stripping reagent. With a sample loading volume of 2.0 mL and an eluent volume of 200 microL, a retention efficiency of 100% and a recovery of 90% are achieved for BSA in the range 6-120 microg, resulting in an enrichment factor of 14. A sampling frequency of 15 h(-1) is achieved, along with a precision of 4.5% at 25 microg x mL(-1) BSA. The practical applicability of this system is demonstrated by processing human whole blood for successive isolation of acidic proteins.
Research Center for Analytical Sciences, Box 332, Northeastern University, Shenyang 110004, China.
Zhuo
Z
Du
Yong-Liang
YL
Yu
Jian-Hua
JH
Wang
D000143:Acids / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification; D002417:Cattle; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D002942:Circular Dichroism; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification
D000143:Acids; D001798:Blood Proteins; D006454:Hemoglobins; D037742:Nanotubes, Carbon; D012710:Serum Albumin, Bovine