Expression, purification and characterization of endo-type chitosanase of Aspergillus sp. CJ22-326 from Escherichia coli.
An endo-chitosanase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an endo-chitosanase activity during viscosimetric assay and TLC analysis. The enzyme had higher chitosanolytic activity than previously reported fungal chitosanases.
Li
Songlin
S
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122 Jiangsu, PR China.
Chen
Liang
L
Wang
Chen
C
Xia
Wenshui
W
D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D001232:Aspergillus fumigatus / Q000201:enzymology / Q000235:genetics; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology*; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014783:Viscosity
D005656:Fungal Proteins; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019868:chitosanase