ErbB receptors modulation in different types of peripheral nerve regeneration.
ErbBs are a family of receptors involved in the trophic maintenance of Schwann cells. Little is known about their expression changes during peripheral nerve regeneration. The aim of this study was thus to investigate variations in ErbBs after end-to-end and end-to-side nerve regeneration in the rat median nerve model. Expression of ErbBs was assessed at 7, 14, and 28 days postoperatively by real-time PCR. Results showed that expression of ErbB1 and ErbB4 mRNAs was downregulated, whereas ErbB3 mRNA was upregulated. No significant changes in ErbB2 mRNA were detected. Our results suggest that ErbBs changes are involved in the molecular response to peripheral nerve injuries.
Department of Animal and Human Biology, Orthopaedic Department, C.T.O. Hospital, Turin, Italy.
Chiara
C
Audisio
Silvia
S
Nicolino
Anna
A
Scevola
Pierluigi
P
Tos
Stefano
S
Geuna
Bruno
B
Battiston
Isabelle
I
Perroteau
D000818:Animals; D015536:Down-Regulation; D066246:ErbB Receptors / Q000235:genetics / Q000502:physiology; D005260:Female; D018773:Genes, erbB-1 / Q000235:genetics / Q000502:physiology; D008475:Median Nerve / Q000503:physiopathology / Q000601:surgery; D020423:Median Neuropathy / Q000235:genetics / Q000503:physiopathology*; D009416:Nerve Regeneration / Q000235:genetics / Q000502:physiology*; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000503:physiopathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000502:physiology*; D020893:Receptor, ErbB-3 / Q000235:genetics / Q000502:physiology; D066247:Receptor, ErbB-4; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000378:metabolism / Q000502:physiology; D015854:Up-Regulation
D066246:ErbB Receptors; D012333:RNA, Messenger; D020794:Receptor Protein-Tyrosine Kinases; D018719:Erb-b2 Receptor Tyrosine Kinases; D020893:Receptor, ErbB-3; D066247:Receptor, ErbB-4; C587689:ERBB4 protein, human; C587691:Erbb4 protein, rat