[Analysis of chemical constituents of anti-myocardial ischemia fraction of Corydalis yanhusuo].
OBJECTIVE
To establish the HPLC fingerprint of anti-myocardial ischemia fraction of Corydalis yanhusuo, and to investigate its chemical constituents by LC-MS-MS.


METHODS
The separation was performed at 30 degrees C on a Dikma Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) by isocratic elution with acetonitrile-water (23:77) (containing 0.8% acetic acid and 0.2% triethylamine) as the mobile phase at a flow rate of 1.0 mL x min(-1), with UV detection at 335 nm.


RESULTS
A satisfactory fingerprint was obtained by HPLC method, 12 common peaks were selected as the fingerprint peaks, and 8 among them were identified according to the chromatographic behavior and mass spectroscopic data by comparison with those of authentic standard.


CONCLUSIONS
This method is simple, sensitive and reliable, can be used to investigate the chemical constituents of anti-myocardial ischemia fraction of C. yanhusuo and control the quality.
Cheng
Xing-ye
XY
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094, China.
Shi
Yue
Y
D002851:Chromatography, High Pressure Liquid; D031311:Corydalis / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D004365:Drugs, Chinese Herbal