[Effect of tetrandrine in combination with droloxifen on the expression of NF-kappaB protein in K562 and K562/A02 cell lines].
OBJECTIVE
To study the effect of tetrandrine (Tet) in combination with droloxifen (DRL) on the expression of nuclear factor kappa B (NF-kappaB) in K562 and K562/A02 cell lines and its reversal mechanism.


METHODS
The activation of NF-kappaB in K562 and K562/A02 cell lines and the effect of Tet or DRL alone or in combination on NF-kappaB protein expression were determined with immunocytochemistry and Western blotting respectively.


RESULTS
(1) K562/A02 cells displayed higher level of NF-kappaB protein expression than K562 cells. (2) The application of Tet or DRL alone or in combination had no effect on NF-kappaB expression in K562 cells at 6 h and 12 h (P > 0.05). (3) Tet and DRL alone or in combination could significantly down-regulate NF-kappaB protein expression in nuclei of K562/A02 cells. The effect was more significant in combination than either alone. This effect was more significant at 12 h than at 6 h.


CONCLUSIONS
(1) Activation of NF-kappaB may be involved in the mechanism of MDR of K562/A02 cell line. (2)Inhibition of NF-kappaB activation may be involved in the reversal of multidrug resistance in K562/A02 cells by Tet and DRL.
Department of Hematology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
Ai-Ling
AL
Su
Bao-An
BA
Chen
Cheng-Yin
CY
Huang
Feng
F
Gao
Jian
J
Cheng
Wen-Lin
WL
Xu
Hui-Ling
HL
Shen
Xin-Chen
XC
Sun
Hong-Yan
HY
Cheng
Jia-Hua
JH
Ding
Chong
C
Gao
Yun-Yu
YY
Sun
Jun
J
Wang
Gang
G
Zhao
Ning-Na
NN
Chen
Hui-Hui
HH
Zhao
D044182:Benzylisoquinolines / Q000494:pharmacology*; D018432:Drug Resistance, Multiple; D006801:Humans; D020014:K562 Cells; D016328:NF-kappa B / Q000378:metabolism*; D013629:Tamoxifen / Q000031:analogs & derivatives* / Q000494:pharmacology
D044182:Benzylisoquinolines; D016328:NF-kappa B; D013629:Tamoxifen; C038345:droloxifene; C009438:tetrandrine