[Study on the relationship between GSTM1, GSTT1 gene polymorphisms and arsenic methylation level].
OBJECTIVE
To investigate the relationship between genetic polymorphisms of GSTT1, GSTM1 and arsenic methylation level.


METHODS
247 residents in an industrial arsenic polluted village were randomly selected as subjects. The genetic polymorphisms of GSTM1 and GSTT1 were detected by multiple PCR method. Urinary inorganic arsenic (iAs), monomethylarsenic acid (MMA) and dimethylarsenic acid (DMA) concentrations were determined by ion chromatogram combined with HG-AFS.


RESULTS
No significant differences in the relative proportion of urinary iAs, MMA and DMA were observed between the individuals with GSTT1 positive genotype and the individuals with GSTT1 null genotype. No significant differences in the relative proportion of urinary iAs, MMA and DMA were observed between the individuals with GSTM1 positive genotype and the individuals with GSTM1 null genotype. And no significant differences in the relative proportion of urinary iAs, MMA and DMA was observed among the individuals with different GSTM1 and GSTT1 associated genotype.


CONCLUSIONS
The polymorphisms of GSTT1 and GSTM1 were not associated with arsenic metabolism level in the studied population.
Institute of Environmental Health and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Liqin
L
Su
Yinlong
Y
Jin
Yibin
Y
Cheng
Shaobin
S
Lin
D000328:Adult; D000393:Air Pollutants / Q000633:toxicity; D001151:Arsenic / Q000378:metabolism / Q000633:toxicity*; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008745:Methylation; D008875:Middle Aged; D011110:Polymorphism, Genetic
D000393:Air Pollutants; D001151:Arsenic; D005982:Glutathione Transferase; C412602:glutathione S-transferase Mu 2