Effects of alpha-tocopherol (vitamin E) on the stability and lipid dynamics of model membranes mimicking the lipid composition of plant chloroplast membranes.
Tocopherol (vitamin E) is widely recognized as a cellular antioxidant. It is essential for human and animal health, but only synthesized in photosynthetic organisms, where it is localized in chloroplast membranes. While many studies have investigated non-antioxidative effects of tocopherol on phospholipid membranes, nothing is known about its effects on membranes containing chloroplast glycolipids. Here, liposomes resembling plant chloroplast membranes were used to investigate the effects of alpha-tocopherol on vesicle stability during freezing and on lipid dynamics. alpha-Tocopherol had a pronounced influence on membrane dynamics and showed strong interactions in its effects on membrane stability during freezing with the cryoprotectant sucrose. alpha-Tocopherol showed maximal effects at low concentrations (around 2mol%), close to its contents in chloroplast membranes.
Hincha
Dirk K
DK
Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany. hincha@mpimp-golm.mpg.de
D003451:Cryoprotective Agents / Q000494:pharmacology; D005615:Freezing; D050356:Lipid Metabolism / Q000187:drug effects; D008081:Liposomes / Q000737:chemistry; D008954:Models, Biological; D018384:Oxidative Stress; D010944:Plants / Q000187:drug effects / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology; D020524:Thylakoids / Q000187:drug effects / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000378:metabolism* / Q000494:pharmacology
D003451:Cryoprotective Agents; D008081:Liposomes; D013395:Sucrose; D024502:alpha-Tocopherol