A linear ion trap mass spectrometer with versatile control and data acquisition for ion/ion reactions.
A linear ion trap (LIT) with electrospray ionization (ESI) for top-down protein analysis has been constructed. An independent atmospheric sampling glow discharge ionization (ASGDI) source produces reagent ions for ion/ion reactions. The device is also meant to enable a wide variety of ion/ion reaction studies. To reduce the instrument's complexity and make it available for wide dissemination, only a few simple electronics components were custom built. The instrument functions as both a reaction vessel for gas-phase ion/ion reactions and a mass spectrometer using mass-selective axial ejection. Initial results demonstrate trapping efficiency of 70% to 90% and the ability to perform proton transfer reactions on intact protein ions, including dual polarity storage reactions, transmission mode reactions, and ion parking.
Soyk
Matthew W
MW
Department of Chemistry, Iowa State University, Ames, Iowa, USA.
Zhao
Qin
Q
Houk
R S
RS
Badman
Ethan R
ER
D045304:Cytochromes c / Q000737:chemistry; D004581:Electronics / Q000295:instrumentation; D004867:Equipment Design; D007477:Ions; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D014357:Trypsin / Q000737:chemistry; D025801:Ubiquitin / Q000737:chemistry
D007477:Ions; D011506:Proteins; D025801:Ubiquitin; D045304:Cytochromes c; D014357:Trypsin