Absorption, migration and kinetics in peripheral blood of orally administered ovalbumin in a mouse model.
Intestinal absorption of food proteins is well known, whereas its physiological significance remains to be investigated. Various amounts (1, 10 and 50 mg) of ovalbumin were orally administered to mice and the blood kinetics were subsequently analyzed by two-site ELISA. The blood ovalbumin concentration consistently reached its maximum (7-90 ng/ml) about 20 min after the oral administration and then gradually decreased in a dose-dependent manner. Only intact (45 kDa) and truncated (40 kDa) ovalbumins were always detected in the blood independently of the administration site, intra-stomach or intra-intestine, while various fragments of the protein were observed in the gastrointestinal lumen after the oral administration. Recognition by a specific monoclonal antibody and an acidic shift of its pI value suggested that the 40-kDa truncated ovalbumin was produced by intracellular limited proteolysis at its C-terminus. Such stable absorption and blood kinetics of undigested ovalbumin in normal mice suggest some sort of physiological significance for the intestinal uptake of intact food proteins.
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi, Japan.
Takeshi
T
Matsubara
Naohito
N
Aoki
Tsutomu
T
Honjoh
Koko
K
Mizumachi
Jun-ichi
J
Kurisaki
Tetsuya
T
Okajima
Daita
D
Nadano
Tsukasa
T
Matsuda
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D041981:Gastrointestinal Tract / Q000378:metabolism; D007120:Immunochemistry; D007263:Infusions, Parenteral; D007700:Kinetics; D051379:Mice; D023421:Models, Animal; D008970:Molecular Weight; D010047:Ovalbumin / Q000008:administration & dosage* / Q000097:blood* / Q000493:pharmacokinetics
D010047:Ovalbumin