[Genetic variations in trnL-F sequence and phylogenetic clustering of Lycoris species].
OBJECTIVE
To identify some closely related Lycoris species and evaluate interspecific relationships among them.


METHODS
The cpDNA trnL-F sequence of 20 taxa representing 15 species of Lycoris and Narcissus tazaetta var. chinensis as one out-group were determined by using direct sequencing of PCR product, and they were analyzed by means of the software of CLUSTRAL and MEGA.


RESULTS
The length of trnL-F of all taxa was 905 - 1 036 bp. When the gaps were always treated as missing, there were 14 variable sites and 10 parsim-info sites, which could be used to identify some species of Lycoris. Four nucleotides inserteions/deletions were significant different among Lycoris and two species of Narcissus. Phylogeny tree was constructed with the maximum parsimony methods by bootstrap test. Three infrageneric clades of all Lycoris species were resolved. The classification was basically consistent with that of morphology except for L. longituba, L. aurea, and L. straminea.


CONCLUSIONS
The tree suggested that L. anhuiensis can not be taken as an independent species, while it may be of a variety or a hybrid of L. longituba. Regarding the hybrid origin species, the materal parent of L. rosea and L. haywardii was revealed to be L. sprengeri. There were some variations in the trnL-F sequence, which were good molecular markers for identification species of Lycoris.
Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing Botanical Garden Mem. Sun Yat-sen, Nanjing 210014, China.
Ju-Hong
JH
Yuan
Shi
S
Sun
Feng
F
Peng
Xu
X
Feng
Yu-Hong
YH
Zheng
Bing
B
Xia
D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal; D017343:Genes, Plant / Q000235:genetics*; D054643:INDEL Mutation; D031426:Lycoris / Q000145:classification* / Q000235:genetics*; D009154:Mutation; D009711:Nucleotides / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004365:Drugs, Chinese Herbal; D009711:Nucleotides