Novel Ree1 regulates the expression of ENO1 via the Snf1 complex pathway in Saccharomyces cerevisiae.
Using cDNA microarray analysis, we found that the mRNA of YJL217W and several other genes related to cell wall organization and biogenesis were up-regulated by galactose in Saccharomyces cerevisiae early during the induction process. YJL217W is also known as REE1 (Regulation of Enolase I). Both the Gal4 regulatory region and the Mac1 binding domain were found on the upstream region of REE1, and the expression of REE1 was up-regulated by galactose but not by glucose. The up-regulation of REE1 by galactose was not observed in the Deltagal4 strain. From the two-hybrid analysis, we found that Ree1 physically interacted with Gal83. Furthermore, from 2-D gel electrophoresis we found that the deletion of REE1 resulted in the up-regulation of Eno1. From Western blotting, we learned that the expression of Eno1 in the Deltaree1 strain was different from that in wild-type strains and that Eno1 expression was not changed by glucose stimulation. Taken together, these results suggest that Ree1p functions in the galactose metabolic pathway via the Gal83 protein and that it may control the level of Eno1p, which is also affected by the Snf1 complex, in S. cerevisiae.
Choi
Il-Dong
ID
School of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Sungbuk-gu, Seoul 136-713, Republic of Korea.
Jeong
Mi-Young
MY
School of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Sungbuk-gu, Seoul 136-713, Republic of Korea.
Ham
Moon-Sik
MS
School of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Sungbuk-gu, Seoul 136-713, Republic of Korea.
Sung
Ha-Chin
HC
School of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Sungbuk-gu, Seoul 136-713, Republic of Korea.
Yun
Cheol-Won
CW
School of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Sungbuk-gu, Seoul 136-713, Republic of Korea. Electronic address: cheolwony@korea.ac.kr.
D001483:Base Sequence; D005690:Galactose / Q000378:metabolism* / Q000494:pharmacology; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
C085569:GAL83 protein, S cerevisiae; C533348:Ree1 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases; C521376:ENO1 protein, S cerevisiae; D010751:Phosphopyruvate Hydratase; D005690:Galactose