Mosaicplasty associated with gene enhanced tissue engineering for the treatment of acute osteochondral defects in a goat model.
OBJECTIVE
To compare single mosaicplasty, mosaicplasty associated with gene enhanced tissue engineering and mosaicplasty associated with the gels of non-gene transduced bone mesenchymal stem cells (BMSCs) in alginate for the treatment of acute osteochondral defects in a goat model.


METHODS
The principle and methods of tissue engineering were used. BMSCs were separated and amplified in vitro, and human transforming growth factor-beta1 (hTGF-beta1) gene was transduced to the cells. Then, the cells were suspended in the alginate. At the same time using mosaicplasty to repair the defects on the medial femoral condyle, the dead space between the cylindrical grafts were filled with the gels of hTGF-beta1 gene transduced BMSCs in alginate. Single mosaicplasty and mosaicplasty associated with the gels of non-gene transduced BMSCs in alginate were compared by the different time observation.


RESULTS
All of the three treatments could repair the acute osteochondral defects. Mosaicplasty associated with gene enhanced tissue engineering had a better integration than single mosaicplasty, and mosaicplasty associated with the gels of non-gene transduced BMSCs in alginate.


CONCLUSIONS
Mosaicplasty associated with tissue engineering could solve the problem of the poor concrescence of the remnant defect and the integration of single mosaicplasty.
Department of Orthopedics, Shanghai Shu Guang Hospital, Shanghai Traditional Chinese Medical University, Shanghai, China. drsunjun@163.com
Jun
J
Sun
Xiao-Kui
XK
Hou
Xu
X
Li
Ting-Ting
TT
Tang
Ru-Ming
RM
Zhang
Yong
Y
Kuang
Meng
M
Shi
D000818:Animals; D019902:Chondrocytes / Q000473:pathology; D004195:Disease Models, Animal; D005269:Femur / Q000601:surgery*; D006041:Goats; D006801:Humans; D007719:Knee Joint / Q000601:surgery*; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D009030:Mosaicism; D023822:Tissue Engineering / Q000379:methods*; D014161:Transduction, Genetic; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
D053773:Transforming Growth Factor beta1