The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green.
Zinc-sensitive fluorescent probes have become increasingly important in the investigation of the cellular roles of zinc. There is, however, little information on how the other transition metals in cells may influence the measurement of zinc. We have characterized in vitro the interaction of the nominal zinc indicators FluoZin-3 and Newport Green with all the cationic transition metals found within cells, Cr, Mn, Fe, Co, and Cu, as well as Ni and Cd, by measuring their dissociation constants. In addition, we have shown how FluoZin-3 can be used to quantify the concentration of copper in a cell-free assay and report that the fluorescence of Newport Green is boosted by both Cu(I) and Fe(II). Furthermore, we have introduced diagnostics for detecting the interference of metals other than zinc with its measurement within cells.
Zhao
Jinfu
J
Department of Biology, 336 BB, University of Iowa, Iowa City, IA 52242, USA.
Bertoglio
Bryan A
BA
Devinney
Michael J
MJ
Dineley
Kirk E
KE
Kay
Alan R
AR
D002104:Cadmium / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007700:Kinetics; D009532:Nickel / Q000737:chemistry; D010084:Oxidation-Reduction; D011083:Polycyclic Compounds / Q000737:chemistry*; D028561:Transition Elements / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
C451182:FluoZin-3; D005456:Fluorescent Dyes; D011083:Polycyclic Compounds; D028561:Transition Elements; D002104:Cadmium; D003035:Cobalt; D003300:Copper; D009532:Nickel; D015032:Zinc