Improvement of bacterial two-hybrid vectors for detection of fusion proteins and transfer to pBAD-tandem affinity purification, calmodulin binding peptide, or 6-histidine tag vectors.
D026481:Blotting, Far-Western; D002148:Calmodulin-Binding Proteins / Q000737:chemistry; D006639:Histidine; D009842:Oligopeptides; D010455:Peptides / Q000737:chemistry; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000032:analysis*; D020798:Two-Hybrid System Techniques
D002148:Calmodulin-Binding Proteins; D009842:Oligopeptides; D010455:Peptides; D011993:Recombinant Fusion Proteins; D006639:Histidine; C085706:FLAG peptide