[Differentially expressed genes involving the correlativity between heart meridian and hypothalamus].
OBJECTIVE
To study the bio-molecule basis of correlativity between Heart Meridian and hypothalamus from gene differential expression.


METHODS
Twenty-eight healthy Sprague-Dawley male rats were divided into 4 groups randomly: normal control, model, Heart Meridian-electroacupuncture (EA) and Lung Meridian-EA groups, with 7 cases in each. Acute myocardial ischemia (AMI) model was duplicated by occlusion of the left anterior descending branch (LAD) of the coronary artery under anesthesia (3% Nemb, 40 mg/kg). EA (1.1 mA, 2 Hz, duration of waves 300 micros) was applied to bilateral "Shenmen" (HT 7)-"Tongli" (HT 5, Heart Meridian), "Taiyuan" (LU 9)-"Lieque" (LU 7, Lung Meridian) separately for 20 min, once daily for 3 days. Then, hypothalamus tissue was taken for differentially expressed gene profiling analysis (Affymetrix GeneChip Rat Genome U 230 Affymetrix).


RESULTS
Compared with model group, among the differentially expressed genes above 2 times in Heart-Meridian-EA group and Lung-Meridian-EA group, 190 and 57 were up-regulated, 34 and 26 down-regulated separately. Compared with Lung-Meridian-EA group, among the 175 differentially expressed genes above 2 times in hypothalamus tissues of Heart-Meridian-EA group, 147 were up-regulated and 28 down-regulated.


CONCLUSIONS
In comparison with EA of Lung Meridian, a different gene expression profile of hypothalamus tissue is involved in the effect of EA of Heart-Meridian on acute myocardial ischemia.
Li
Meng
M
Center for Researching Meridian-Viscera Correlation, Anhui College of Chinese Medicine, Hefei 230038, China.
Long
Di-he
DH
He
Lu
L
Hu
Ling
L
Wang
Ke-ming
KM
Wu
Zi-jian
ZJ
Cai
Rong-lin
RL
Zhou
Yi-ping
YP
D000818:Animals; D015671:Electroacupuncture; D015870:Gene Expression; D020869:Gene Expression Profiling; D006321:Heart / Q000503:physiopathology*; D006801:Humans; D007031:Hypothalamus / Q000503:physiopathology*; D008297:Male; D016740:Meridians; D017202:Myocardial Ischemia / Q000503:physiopathology / Q000628:therapy*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley