A noncytotoxic DsRed variant for whole-cell labeling.
A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.
Strack
Rita L
RL
Department of Biochemistry and Molecular Biology, The University of Chicago, Gordon Center W238, Chicago, Illinois 60637, USA.
Strongin
Daniel E
DE
Bhattacharyya
Dibyendu
D
Tao
Wen
W
Berman
Allison
A
Broxmeyer
Hal E
HE
Keenan
Robert J
RJ
Glick
Benjamin S
BS
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis* / Q000633:toxicity; D008969:Molecular Sequence Data; D013194:Staining and Labeling / Q000379:methods*
D008164:Luminescent Proteins; C413663:fluorescent protein 583