Evaluation of a rapid method for the quantitative analysis of fatty acids in various matrices.
A simplified method for quantitative analysis of fatty acids in various matrices by gas chromatography is proposed as an alternative to the conventional method and the variables of the protocol examined to optimize the processing conditions. The modified method involves direct methylation of fatty acids in homogenized samples with boron trihalide (BF(3) or BCl(3) in methanol) followed by extraction with hexane. The addition of hexane to the reaction mixture after the methylation process can enhance the efficiency of fatty acid methylation and is critical for those samples that contain high levels of triglycerides. A mechanism underlying this effect is proposed.
Araujo
Pedro
P
National Institute of Nutrition and Seafood Research, N-5817 Bergen, Norway. pedro.araujo@nifes.no
Nguyen
Thu-Thao
TT
Frøyland
Livar
L
Wang
Jingdong
J
Kang
Jing X
JX
D000818:Animals; D001896:Boron Compounds / Q000737:chemistry; D001923:Brain Chemistry; D002849:Chromatography, Gas / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods; D004912:Erythrocytes / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D005399:Fishes / Q000097:blood; D006586:Hexanes / Q000737:chemistry; D006801:Humans; D008099:Liver / Q000737:chemistry; D008297:Male; D008745:Methylation; D008956:Models, Chemical; D009132:Muscles / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000737:chemistry
D001896:Boron Compounds; D005227:Fatty Acids; D006586:Hexanes; D012972:Sodium Hydroxide