[Monocyte chemoattractant protein-1 regulations of human gingival fibroblasts by Porphyromonas gingivalis with different fimA genotypes].
OBJECTIVE
To investigate the mechanism of monocyte chemoattractant protein-1 (MCP-1) regulations of human gingival fibroblasts (HGF) by challenge of Porphyromonas gingivalis (Pg) with different fimA genotypes.


METHODS
Pg ATCC 33277 (type I), WCSP115 (type II), WCSP1.5 (type III), W83 (type IV) were assessed for their inductions of MCP-1 expression in HGF. MCP-1 mRNA levels of HGF were determined by real-time RT-PCR and MCP-1 protein levels in culture supernatant by ELISA at different time intervals (1 h, 3h, 6h and 12h) following continuous co-culture of bacteria with HGF.


RESULTS
MCP-1 mRNA and protein levels were both up-regulated when HGF co-cultured with different Pg fimA genotypes. Type II was stronger than other fimA genotypes, HGF expressed significantly great amount of MCP-1 mRNA [(25.75 +/- 3.12)-(326.69 +/- 35.35)] and protein [(178.20 +/- 46.20)-(443.46 82.19) ng/L] for different time periods; While Type III was weaker than other fimA genotypes, and the level of MCP-1 mRNA was [ (4.16 +/- 0.82)-(94.17 +/- 18.56)] and protein [(86.95 +/- 23.90)-(264.01 +/- 28.59) ng/L](P < 0.05).


CONCLUSIONS
fimA genotypes of Pg are related with the inductions of MCP-1, which might indicate fimA genotype is associated with pathogenesis of Pg.
Zhao
Lei
L
State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Wu
Ya-fei
YF
Ouyang
Yu-ling
YL
Zhang
Jing-yi
JY
Chen
Bin
B
D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000235:genetics*; D005838:Genotype; D005881:Gingiva / Q000166:cytology / Q000382:microbiology*; D006801:Humans; D016966:Porphyromonas gingivalis / Q000235:genetics*
C111835:CCL2 protein, human; D018932:Chemokine CCL2; C063055:fimbrillin; D036781:Fimbriae Proteins