Screening for acetylcholinesterase inhibitory activity in cyanobacteria of the genus Nostoc.
Fifty-four cyanobacterial strains of the genus Nostoc from different habitats were screened for acetylcholinesterase inhibitory activity. Water-methanolic extracts from freeze-dried biomasses were tested for inhibitory activity using Ellman's spectrophotometric method. Acetylcholinesterase inhibitory activity higher than 90% was found in the crude extracts of Nostoc sp. str. Lukesova 27/97 and Nostoc ellipsosporum Rabenh. str. Lukesova 51/91. Extracts from Nostoc ellipsosporum str. Lukesova 52/91 and Nostoc linckia f. muscorum (Ag.) Elenk. str. Gromov, 1988, CALU-980 inhibited AChE activity by 84.9% and 65.3% respectively. Moderate AChE inhibitory activity (29.1-37.5%) was found in extracts of Nostoc linckia Roth. str. Gromov, 1962/10, CALU-129, Nostoc muscorum Ag. str. Lukesova 127/97, Nostoc sp. str. Lhotsky, CALU-327 and Nostoc sp. str. Gromov, CALU-998. Extracts from another seven strains showed weak anti-AChE activities. The active component responsible for acetylcholinesterase inhibition was identified in a crude extract of Nostoc sp. str. Lukesova 27/97 using HPLC and found to occur in one single peak.
Zelík
Petr
P
Faculty of Chemistry, Institute of Food Chemistry and Biotechnology, Brno University of Technology, Brno, Czech Republic.
Lukesová
Alena
A
Voloshko
Ludmila N
LN
Stys
Dalibor
D
Kopecký
Jirí
J
D000110:Acetylcholinesterase / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D000458:Cyanobacteria / Q000737:chemistry / Q000378:metabolism; D046937:Nostoc / Q000737:chemistry* / Q000145:classification / Q000378:metabolism
D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase