title: Impact of food disinfection on beneficial biothiol contents in strawberry.
abstract: In this study, the impact of four food disinfectants including hydrogen peroxide, free chlorine, and gaseous- and aqueous-phase ozone with industrial doses on the concentration of biothiol compounds gamma-glutamylcysteinylglycine (GSH) and cystein (CYS) in strawberry was investigated for 1, 5, 15, 30, and 60 or 120 min. Additionally, the amount of oxidized glutathione (GSSG) was analyzed for calculation of the GSH/GSSG ratio as an indicator of oxidative stress. After this treatment, thiol contents of strawberry samples were examined using high-performance liquid chromatography (HPLC) technique. According to the results of measurements, free chlorine treatment for only 60 min significantly decreased CYS content in strawberry (p < 0.05). A significant decline in the GSH/GSSG ratio was also observed when H2O2 was applied for all time intervals except for 1 min (p < 0.05). However, aqueous-phase ozone treatment did not significantly affect the thiol levels (p < 0.05). In conclusion, this study may provide optimum disinfection methods for strawberry to minimize loss of beneficial biothiols.
authors.affiliation: Muhendislik Fakultesi, Gida Muhendisligi Bolumu, Sakarya Universitesi, Esentepe, Sakarya, Turkey. omcad@sakarya.edu.tr
authors.forename: Omca
authors.initials: O
authors.lastname: Demirkol
authors.forename: Arzu
authors.initials: A
authors.lastname: Cagri-Mehmetoglu
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Qiang
authors.forename: Nuran
authors.initials: N
authors.lastname: Ercal
authors.forename: Craig
authors.initials: C
authors.lastname: Adams
mesh_terms: D003545:Cysteine / Q000032:analysis / Q000378:metabolism*; D004202:Disinfectants / Q000494:pharmacology*; D005519:Food Preservation / Q000379:methods*; D031985:Fragaria / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D005978:Glutathione / Q000032:analysis / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D004202:Disinfectants; D005978:Glutathione; D003545:Cysteine; D019803:Glutathione Disulfide