title: A novel design of multifunctional integrated cell-based biosensors for simultaneously detecting cell acidification and extracellular potential.
abstract: The paper discussed a novel design of multifunctional cell-based biosensors for simultaneously detecting cell acidification and extracellular potential. Employing living cells such as cardiac myocytes as a source for the light addressable potentiometric sensor (LAPS) array, this cell-based biosensor was able to monitor both the acidification and extracellular potential in parallel. For LAPS array fabrication, part of the silicon base was heavily doped with boron to form separate testing areas. Detecting system was built involving lock-in amplifier and digital demodulation with FFT methods. This LAPS array showed a good sensitivity of 53.9 mV/pH to H(+) with good linearity. Each testing area for extracellular potential detection was decreased to 200 microm x 200 microm in size to obtain a better sensitivity. Experiment results showed that this LAPS array could monitor the acidification of cells as well as the extracellular potential with good sensitivity. This novel integrated biosensor will be useful for multi-parameter extracellular monitoring and can possibly be a platform for drug screening.
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.forename: Hua
authors.initials: H
authors.lastname: Cai
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Lidan
authors.initials: L
authors.lastname: Xiao
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018511:Systems Integration