Heat shock protein 60 expression in heart, liver and kidney of broilers exposed to high temperature.
The objective of this study was to investigate the expression and localization of HSP60 in the heart, liver, and kidney of acutely heat-stressed broilers at various stressing times. The plasma creatine kinase (CK) and glutamic pyruvic transaminase (GPT) concentrations statistic increased following heat stress. After 2h of heat stress, the tissues showed histopathological changes. Hsp60 expressed mainly in the cytoplasm of parenchyma cells heat stress. The intensity of the cytoplasmic staining varied and exhibited an organ-specific distribution pattern. Hsp60 levels in the hearts of heat-stressed chickens gradually increased at 1h (p<0.05) and peaked (p<0.05) at 5h; Hsp60 levels in the liver gradually decreased at 3h (p<0.05); Hsp60 levels in the kidney had no fluctuation. It is suggested that Hsp60 expression is tissue-specific and this may be linked to tissue damage in response to heat stress. The Hsp60 level is distinct in diverse tissues, indicating that Hsp60 may exert its protective effect by a tissue- and time-specific mechanism.
Yan
Jianyan
J
College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, 210095, Nanjing, Jiangsu Province, China.
Bao
Endong
E
Yu
Jimian
J
D000064:Acclimatization; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D018834:Chaperonin 60 / Q000378:metabolism*; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D003402:Creatine Kinase / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006799:Housing, Animal; D006813:Humidity; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009928:Organ Specificity
D018834:Chaperonin 60; D000410:Alanine Transaminase; D003402:Creatine Kinase