[Gene polymorphism of the endothelial nitric oxide synthase enzyme and pulmonary hypertension in patient with chronic obstructive pulmonary disease].
OBJECTIVE
To investigate the relationship between the gene polymorphism of the endothelial nitric oxide synthase enzyme (eNOSG894T) with pulmonary hypertension of Chinese patient with chronic obstructive pulmonary disease (COPD).


METHODS
Fifty normal volunteers (29 male, 21 female, mean age 67 +/- 3) and 50 patients with COPD complicated by pulmonary hypertension (31 male, 19 female, mean age 69 +/- 10) were included in the study. Lung function, arterial blood gases, and echocardiographic examination were performed. The genotype of eNOSG894T was determined with PCR.


RESULTS
The frequencies of GT + FT genotype and T allele were significantly greater in the patients compared to the controls. The levels of NOx and NOx/ET were lower in the GT + T genotype [(48 +/- 8) micromol/L, (0.51 +/- 0.20)] than in the GG genotype [(60 +/- 24) micromol/L, (1.43 +/- 0.64)] of COPD. The level of ET was higher in the GT + TT genotype [(104 +/- 38) microg/L] than in the GG genotype [(50 +/- 26) microg/L] of COPD patients. The mean systolic pulmonary artery pressure was higher in the GT + TT genotype [(57 +/- 15) mm Hg, 1 mm Hg = 0.133 kPa] than in the GG genotype [(47 +/- 10) mm Hg] of COPD patients. In stepwise logistic regression analysis for predicting pulmonary artery pressure, the polymorphism of eNOS gene, the level of Nox and smoking were found to be independent variables.


CONCLUSIONS
The G894T polymorphism of eNOS gene is associated with pulmonary hypertension of COPD in a Chinese population. T allele may be involved in the etiology of pulmonary hypertension with COPD by reducing NO release of the endothelium.
Sun
Li-Rong
LR
Department of Respiratory Medicine, Second Affiliated Hospital, Baotou Medical College, Baotou 014030, China. roseslr@163.com
Wang
Cong
C
Wu
Ai-Qing
AQ
Yan
Bao-Min
BM
Li
Dong-Mei
DM
Zhang
Jian-Ling
JL
Wang
Guo-Jun
GJ
Zhang
Yi
Y
Li
Xue-Qin
XQ
Zhang
Qiao-Zhi
QZ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006976:Hypertension, Pulmonary / Q000150:complications / Q000201:enzymology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic; D029424:Pulmonary Disease, Chronic Obstructive / Q000150:complications / Q000201:enzymology / Q000235:genetics*
C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III