Metabolite imaging using matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS).
We describe here the use of a hybrid ionization approach, matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS) in bioimaging. ME-SALDI combines the strengths of traditional matrix-assisted laser desorption/ionization (MALDI) and SALDI and enables successful MS imaging of low-mass species with improved detection sensitivity. Using 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as the MS standard, MS performances of MALDI, SALDI, and ME-SALDI are systematically compared. The analyte desorption and ionization mechanism in ME-SALDI is qualitatively speculated based on the observation of significantly reduced matrix background and improved survival yields of molecular ions. Improvements in detection sensitivity of low-mass species using ME-SALDI over MALDI in imaging are demonstrated with mouse heart and brain tissues.
Liu
Qiang
Q
Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA.
Xiao
Yongsheng
Y
Pagan-Miranda
Coral
C
Chiu
Yu Matthew
YM
He
Lin
L
D000818:Animals; D001923:Brain Chemistry; D002531:Cerebellum / Q000737:chemistry; D054022:Cerebrum / Q000737:chemistry; D003952:Diagnostic Imaging / Q000379:methods*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005260:Female; D051379:Mice; D009206:Myocardium / Q000737:chemistry*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D004134:Dimyristoylphosphatidylcholine