[Study on the classification of gonococcal island of different genotypes and effects of sac-4 gene on serum resistance of Neisseria gonorrhoeae].
OBJECTIVE
Using molecular methods to study the relationship between genotypes and serum resistance of Neisseria gonorrhoeae in Wuhan area.


METHODS
NG-mast and serum bactericidal assays at the molecular level were used to differentiate the 46 strains which were isolated from the outpatients of sexually transmitted disease clinics and the relationship between different genotypes while phenotypes was also studied.


RESULTS
80.43% of the 46 strains contained the island and we were able to define three different combinations of genes in the isolates. Results from serum bactericidal assays showed that all 9 sac-4+ strains did not provide any serum resistance.


CONCLUSIONS
Different isolates carried different gonococcal genetic islands (pathogenicity island) and certain phenotypes. There were no sobious relationship between sac-4 gene and serum resistance of Neisseria gonorrhoeae.
Chen
Hong-Xiang
HX
Department of Dermatology, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Wu
Zhi-Hong
ZH
Chen
Rong-Yi
RY
Shuai
Jun
J
Xu
Li
L
Yu
Ying
Y
Tu
Ya-Ting
YT
D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001770:Blood Bactericidal Activity / Q000235:genetics; D002584:Cervix Uteri / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000302:isolation & purification; D012703:Serotyping; D014521:Urethra / Q000382:microbiology; D055815:Young Adult
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial