title: Fast gas chromatography characterisation of purified semiochemicals from essential oils of Matricaria chamomilla L. (Asteraceae) and Nepeta cataria L. (Lamiaceae).
abstract: The chemical composition of Matricaria chamomilla L. and Nepeta cataria L. essential oils was determined by GC-MS on an apolar stationary phase by comparison of the characteristic fragmentation patterns with those of the Wiley 275L database. The GC-MS chromatograms were compared with those obtained by fast GC equipped with a direct resistively heated column (Ultra Fast Module 5% phenyl, 5 mx 0.1 mm, 0.1 microm film thickness). Analytical conditions were optimised to reach a good peak resolution (split ratio=1:100), with analysis time lower than 5 min versus 35-45 min required by conventional GC-MS. The fast chromatographic method was completely validated for the analysis of mono- and sesquiterpene compounds. Essential oils were then fractionated by column chromatography packed with silica gel. Three main fractions with high degree of purity in E-beta-farnesene were isolated from the oil of M. chamomilla. One fraction enriched in (Z,E)-nepetalactone and one enriched in beta-caryophyllene were obtained from the oil of N. cataria. These semiochemical compounds could act as attractants of aphid's predators and parasitoids.
authors.affiliation: Department of Analytical Chemistry, Gembloux Agricultural University, Passage des Déportés 2, B-5030 Gembloux, Belgium.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Heuskin
authors.forename: Bruno
authors.initials: B
authors.lastname: Godin
authors.forename: Pascal
authors.initials: P
authors.lastname: Leroy
authors.forename: Quentin
authors.initials: Q
authors.lastname: Capella
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Wathelet
authors.forename: François
authors.initials: F
authors.lastname: Verheggen
authors.forename: Eric
authors.initials: E
authors.lastname: Haubruge
authors.forename: Georges
authors.initials: G
authors.lastname: Lognay
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D031168:Matricaria / Q000737:chemistry*; D027521:Nepeta / Q000737:chemistry*; D009822:Oils, Volatile / Q000032:analysis*; D010675:Pheromones / Q000032:analysis* / Q000302:isolation & purification; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D009822:Oils, Volatile; D010675:Pheromones; D010938:Plant Oils