Two-dimensional DOSY experiment with Excitation Sculpting water suppression for the analysis of natural and biological media.
The Bipolar Pulse Pair Stimulated Echo NMR pulse sequence was modified to blend the original Excitation Sculpting water signal suppression. The sequence is a powerful tool to generate rapidly, with a good spectrum quality, bidimensional DOSY experiments without solvent signal, thus allowing the analysis of complex mixtures such as plant extracts or biofluids. The sequence has also been successfully implemented for a protein at very-low concentration in interaction with a small ligand, namely the salivary IB5 protein binding the polyphenol epigallocatechine gallate. The artifacts created by this sequence can be observed, checked and removed thanks to NPK and NMRnotebook softwares to give a perfect bidimensional DOSY spectrum.
Balayssac
Stéphane
S
Groupe de RMN Biomédicale, Laboratoire SPCMIB (UMR CNRS 5068), Université Paul Sabatier, Toulouse, France.
Delsuc
Marc-André
MA
Gilard
Véronique
V
Prigent
Yann
Y
Malet-Martino
Myriam
M
D000465:Algorithms; D016477:Artifacts; D001704:Biopolymers / Q000032:analysis* / Q000737:chemistry*; D045424:Complex Mixtures / Q000032:analysis* / Q000737:chemistry*; D003198:Computer Simulation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*
D001704:Biopolymers; D045424:Complex Mixtures; D014867:Water