title: Emergence of nosocomial Mycobacterium massiliense infection in Goiás, Brazil.
abstract: A cluster of surgical site infection cases after arthroscopic and laparoscopic procedures occurred between 2005 and 2007 in Goiânia, in the central region of Brazil. Nontuberculous mycobacteria (NTM) were isolated from samples (exudates from cutaneous abscesses) from 18 patients of seven private hospitals. There were no reports of post-surgical arthroscopic and laparoscopic mycobacterial infections in Goiânia apart from this period. The 18 isolates were identified as Mycobacterium massiliense by PCR-restriction digestion of the hsp65 gene, pulsed-field gel electrophoresis (PFGE) comparisons, and rpoB partial gene sequencing. All isolates were typed as a single clone, indicating that they have the same origin, which suggests a common source of infection for all patients.
authors.affiliation: Departamento de Microbiologia, Imunologia, Parasitologia e Patologia, Universidade Federal de Goiás, Goiás, Brazil.
authors.forename: Alessandra Marques
authors.initials: AM
authors.lastname: Cardoso
authors.forename: Eduardo
authors.initials: E
authors.lastname: Martins de Sousa
authors.forename: Cristina
authors.initials: C
authors.lastname: Viana-Niero
authors.forename: Fernando
authors.initials: F
authors.lastname: Bonfim de Bortoli
authors.forename: Zilah Cândida
authors.initials: ZC
authors.lastname: Pereira das Neves
authors.forename: Sylvia Cardoso
authors.initials: SC
authors.lastname: Leão
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Junqueira-Kipnis
authors.forename: André
authors.initials: A
authors.lastname: Kipnis
mesh_terms: D000038:Abscess / Q000453:epidemiology / Q000382:microbiology; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001938:Brazil / Q000453:epidemiology; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D016000:Cluster Analysis; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000453:epidemiology* / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D017192:Skin Diseases, Bacterial / Q000453:epidemiology / Q000382:microbiology; D013530:Surgical Wound Infection / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; C074119:heat-shock protein 65, Mycobacterium; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D018833:Chaperonins