[Effect of dibromothymoquinone on chlorophyll a fluorescence in Chlamydomonas reinhardtii cells incubated in complete or sulfur-depleted medium].
The influence of dibromothymoquinone on chlorophyll fluorescence was studied in Chlamydomonas reinhardtii cells using PAM and PEA fluorometers. The reagent affected differently control cells incubated in complete medium and S-starved cells. Thus, the fluorescence yield in the control essentially increased in the presence of dibromothymoquinone, which can be due to the inactivation of light-harvesting complex II protein kinase, followed by the suppression of membrane transition from high-fluorescence state 1 to low-fluorescence state 2. On the contrary, S-starved cells with membranes in state 2 showed a lower fluorescence yield in the presence of dibromothymoquinone than without it. The JIP test of OJIP fluorescence transients suggests that dibromothymoquinone inhibits both light-harvesting complex II kinase and photosynthetic electron transport when added to control, while in starved cells, it acts predominantly as an electron acceptor.
Volgusheva
A A
AA
Kukarskikh
G P
GP
Antal
T K
TK
Lavrukhina
O G
OG
Krendeleva
T E
TE
Rubin
A B
AB
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000737:chemistry / Q000378:metabolism*; D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media / Q000494:pharmacology; D003991:Dibromothymoquinone / Q000493:pharmacokinetics*; D004579:Electron Transport / Q000187:drug effects; D005453:Fluorescence; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013455:Sulfur / Q000494:pharmacology
D003470:Culture Media; D045342:Light-Harvesting Protein Complexes; D015800:Protozoan Proteins; D002734:Chlorophyll; D003991:Dibromothymoquinone; D013455:Sulfur; D011494:Protein Kinases