Inductions of histone H3 acetylation at lysine 9 on SGLT1 gene and its expression by feeding mice a high carbohydrate/fat ratio diet.
OBJECTIVE
We examined in this study whether histone H3 acetylations on jejunal NA(+)/glucose transport-1 (SGLT1) gene are associated with induction of its gene by feeding mice a high carbohydrate/fat ratio diet.


METHODS
The chromatin immunoprecipitation (ChIP) assays using antibodies of acetylated histone H3 were performed in jejunum of mice fed a high carbohydrate/fat ratio diet or a low carbohydrate/fat ratio for 7 d.


RESULTS
The acetylations of histone H3 of lysine 9/14 were highly detected on the SGLT1 gene and the levels were enhanced by feeding mice a high carbohydrate/fat ratio diet. Histone H3 acetylation at lysine 9 was enhanced by feeding a high carbohydrate/fat ratio diet, whereas that at lysine 14 was not enhanced significantly.


CONCLUSIONS
These observations indicate that induction of SGLT1 gene expression by feeding a high carbohydrate/fat ratio diet is associated with acetylation of histone H3 at lysine 9 on the SGLT1 gene.
Honma
Kazue
K
Laboratory of Nutritional Physiology, Graduate School of Nutritional and Environmental Sciences and Global COE, The University of Shizuoka, Shizuoka, Japan.
Mochizuki
Kazuki
K
Goda
Toshinao
T
D000107:Acetylation; D000818:Animals; D047369:Chromatin Immunoprecipitation; D004040:Dietary Carbohydrates / Q000008:administration & dosage* / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D006657:Histones / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D051269:Sodium-Glucose Transporter 1 / Q000235:genetics* / Q000378:metabolism*
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D006657:Histones; C495675:Slc5a1 protein, mouse; D051269:Sodium-Glucose Transporter 1