Enhanced enzymatic saccharification of rice straw by microwave pretreatment.
In this study, Box-Behnken design and response surface methodology were employed to plan experiments and optimize the microwave pretreatment of rice straw. Experimental results show that microwave intensity (MI), irradiation time (IT) and substrate concentration (SC) were main factors governing the enzymatic saccharification of rice straw. The maximal efficiencies of cellulose, hemicellulose and total saccharification were respectively increased by 30.6%, 43.3% and 30.3% under the optimal conditions of MI 680 W, IT 24 min and SC 75 g/L. The chemical composition analysis of straw further confirmed that microwave pretreatment could disrupt the silicified waxy surface, break down the lignin-hemicellulose complex and partially remove silicon and lignin.
Ma
Huan
H
Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, China.
Liu
Wei-Wei
WW
Chen
Xing
X
Wu
Yue-Jin
YJ
Yu
Zeng-Liang
ZL
D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D003198:Computer Simulation; D004307:Dose-Response Relationship, Radiation; D004789:Enzyme Activation / Q000528:radiation effects; D008872:Microwaves; D008954:Models, Biological; D012275:Oryza / Q000737:chemistry* / Q000528:radiation effects*; D035261:Plant Components, Aerial / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage
D002241:Carbohydrates; D002482:Cellulose; D002480:Cellulase