The genome and proteome of the Kluyvera bacteriophage Kvp1--another member of the T7-like Autographivirinae.
BACKGROUND
Kluyvera, a genus within the family Enterobacteriaceae, is an infrequent cause of human infections. Bacteriophage Kvp1, the only bacteriophage isolated for one of its species, Kluyvera cryocrescens, is a member of the viral family Podoviridae.


RESULTS
The genome of Kvp1, the first Kluyvera cryocrescens-specific bacteriophage, was sequenced using pyrosequencing (454 technology) at the McGill University and Genome Québec Innovation Centre. The two contigs were closed using PCR and the sequence of the terminal repeats completed by primer walking off the phage DNA. The phage structural proteome was investigated by SDS-PAGE and mass spectrometry.


CONCLUSIONS
At 39,472 bp, the annotated genome revealed a closer relationship to coliphage T3 than T7 with Kvp1 containing homologs to T3 early proteins S-adenosyl-L-methionine hydrolase (0.3) and protein kinase (0.7). The quantitative nature of the relationships between Kvp1 and the other members of the T7-like virus genus (T7, T3, phiA1122, phiYeO3-12, Berlin, K1F, VP4 and gh-1) was confirmed using CoreGenes.
Lingohr
Erika J
EJ
Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, Guelph, ON, Canada. Andrew_Kropinski@phac-aspc.gc.ca. Erika_Lingohr@phac-aspc.gc.ca
Villegas
Andre
A
She
Yi-Min
YM
Ceyssens
Pieter-Jan
PJ
Kropinski
Andrew M
AM
D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D016679:Genome, Viral; D044086:Kluyvera / Q000821:virology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D017902:Podoviridae / Q000737:chemistry* / Q000235:genetics*; D020543:Proteome / Q000032:analysis*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013604:T-Phages / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
D004279:DNA, Viral; D020543:Proteome; D014764:Viral Proteins