[Effect of inhibitor of differentiation-1 on murine dendritic cell sarcoma cells].
OBJECTIVE
To investigate the effect of down-expression of inhibitor of differentiation-1 (Id-1) on the differentiation of dendritic cell sarcoma (DCS) cells in vitro.


METHODS
Down-regulation of the expression of Id-1 in DCS cells was performed by RNAi, and confirmed by protein and mRNA quantitative analyses. Cellular differentiation and biological behavior including malignant phenotypes of the cells were evaluated. All experiments included negative (no treatment group and no-target siRNA) and positive (induction-differentiation drug sodium butyrate) controls.


RESULTS
When the expression of Id-1 was down regulated, the DCS cells showed more mature morphology including cell enlargement, longer cellular extensions, more branches, and decreased nuclear/plasma ratio. Differentiation marker expression (Id-2 and CD86) was also increased. RNAi treated cells at 24 and 48 hours, showed increase percentage of cells at G0/G1 phase and less cells at S phase (P < 0.01). Importantly, the abilities of cell proliferation, colony formation and invasiveness were significantly decreased (P < 0.01), as evidenced by MTT, colony formation and transwell assays respectively.


CONCLUSIONS
RNAi inhibition of Id-1 protein can induce differentiation of malignant solid tumor cells along with reversion of their malignant phenotype.
Bian
Xiao-Cui
XC
Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
Liu
Yu-Qin
YQ
Gu
Bei
B
Feng
Hai-Liang
HL
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects*; D015536:Down-Regulation; D051796:Inhibitor of Differentiation Proteins / Q000494:pharmacology*; D051379:Mice; D014407:Tumor Cells, Cultured
D051796:Inhibitor of Differentiation Proteins