The implication of N-acetylglucosaminyltransferase V expression in gastric cancer.
OBJECTIVE
N-acetylglucosaminyltransferase V (GnT-V) is a key enzyme that catalyzes beta(1-6) branching of N-acetylglucosamine on N-glycan of cell proteins, some of which are linked with metastasis. GnT-V expression was studied immunohistochemically in gastric cancer to compare clinicopathological parameters and evaluate the role of GnT-V in the prognosis of gastric cancer.


METHODS
Immunohistochemistry was carried out to detect GnT-V expression in 50 advanced gastric cancer tissues where the depth of invasion exceeded the subserosa, and the relationship between GnT-V expression and various clinicopathological factors, including survival, was analyzed.


RESULTS
GnT-V was expressed in 23 (46%) gastric cancer tissues. GnT-V expression was significantly correlated with lymph node metastases, peritoneal dissemination and liver metastases, respectively (p = 0.005, p = 0.013 and p = 0.023). Patients with GnT-V-positive gastric cancer had a significantly shorter survival than those without GnT-V expression (5-year survival rate: 31.2 and 54.4%, respectively; p = 0.045).


CONCLUSIONS
GnT-V expression is correlated with a poor prognosis in gastric cancer patients due to metastases.
Tian
Hai
H
Department of Molecular Pathology, Graduate School of Medicine and Health Science, Osaka University, Suita, Osaka, Japan.
Miyoshi
Eiji
E
Kawaguchi
Naomasa
N
Shaker
Mohammed
M
Ito
Yasuhiro
Y
Taniguchi
Naoyuki
N
Tsujimoto
Masahiko
M
Matsuura
Nariaki
N
D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000201:enzymology / Q000556:secondary; D008297:Male; D017351:N-Acetylglucosaminyltransferases / Q000096:biosynthesis*; D009362:Neoplasm Metastasis; D010534:Peritoneal Neoplasms / Q000201:enzymology / Q000556:secondary; D011379:Prognosis; D013160:Splenic Neoplasms / Q000201:enzymology / Q000556:secondary; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D015996:Survival Rate
D014408:Biomarkers, Tumor; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase