title: MicroRNA expression profile of bronchioalveolar stem cells from mouse lung.
abstract: Increasing evidence has suggested that bronchioalveolar stem cell (BASC) is the progenitor cells of lung cancer stem cells. However, the mechanisms by which self-renewal of BSACs is controlled and how BASCs turn into cancer stem cells still remains to be unknown. In the present study, we successfully isolated bronchioalveolar stem cells (BASCs) from mouse lung using FACS. These BASCs were characterized by clonal growth, self-renewal and high capacity for differentiation, suggesting that these BASCs are indeed stem cells. We investigated the microRNA (miRNA) expression profile of these BASCs using miRNA array and quantitative RT-PCR. We discovered that BASCs possessed a unique miRNA profile, with altered expression of several microRNAs, such as miR-142-3p, miR-451, miR-106a, miR-142-5p, miR-15b, miR-20a, miR-106b, miR-25, miR-486, in BASCs compared to control cells. Our results suggest that microRNAs might play important roles in maintaining the self-renewal capacity of BASCs, and suggest the intriguing possibility that aberrant expression of microRNAs could involved in turning BASCs into lung cancer stem cells.
authors.affiliation: Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
authors.forename: Shen
authors.initials: S
authors.lastname: Qian
authors.affiliation: Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Ding
authors.affiliation: Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
authors.forename: Rongkai
authors.initials: R
authors.lastname: Xie
authors.affiliation: Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
authors.forename: Jiang-Hong
authors.initials: JH
authors.lastname: An
authors.affiliation: Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
authors.forename: Xu-Jun
authors.initials: XJ
authors.lastname: Ao
authors.affiliation: Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Zhao
authors.affiliation: Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Sun
authors.affiliation: Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
authors.forename: Yu-Zhong
authors.initials: YZ
authors.lastname: Duan
authors.affiliation: Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China. Electronic address: zhengtangchen@yahoo.com.cn.
authors.forename: Zheng-Tang
authors.initials: ZT
authors.lastname: Chen
authors.affiliation: Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China. Electronic address: oncology_bozhu@yahoo.com.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D020869:Gene Expression Profiling; D008175:Lung Neoplasms / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D035683:MicroRNAs