The association between dilated cardiomyopathy and RTN4 3'UTR insertion/deletion polymorphisms.
BACKGROUND
The Nogo isoforms A, B and C are members of the reticulon family of proteins. Nogo-B is found in most tissues, especially highly expressed in endothelial and smooth muscle cells of the vessel wall, and Nogo-B is a regulator of cell migration in vitro and vascular remodeling in vivo. The TATC and CAA 3'UTR insertion/deletion polymorphisms of the RTN4, the gene encoding Nogo isoforms A, B and C, have been linked to schizophrenia and depression, but data were inconsistent. However, it is unclear whether these polymorphisms are associated with dilated cardiomyopathy (DCM).


METHODS
A total of 159 DCM patients and 215 control subjects were recruited in this study. The RTN4 TATC and CAA insertion/deletion genotypes were determined using PCR-polyacrylamide gel electrophoresis.


RESULTS
Frequencies of (TATC)(2) allele and (TATC)(2)/(TATC)(2) genotype were significantly different from that in healthy controls (P = 0.045, OR = 1.356, 95% CI = 1.006-1.827 and P = 0.021, OR = 2.094, 95% CI = 1.113-3.940, respectively). No significant differences in CAA insertion/deletion genotype and allele frequencies were observed between the DCM and controls.


CONCLUSIONS
These data provide evidence that RTN4 allele (TATC)(2) and (TATC)(2)/(TATC)(2) genotype are associated with DCM.
Zhou
Bin
B
Laboratory of Molecular Translational Medicine, West China Second University Hospital, Sichuan University, Chengdu 610041, PR China.
Rao
Li
L
Li
Yi
Y
Gao
Linbo
L
Li
Cui
C
Chen
Yu
Y
Xue
Hui
H
Liang
Weibo
W
Lv
Meili
M
Song
Yaping
Y
Peng
Ying
Y
Zhang
Lin
L
D020413:3' Untranslated Regions / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002311:Cardiomyopathy, Dilated / Q000235:genetics*; D002648:Child; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D054643:INDEL Mutation; D008297:Male; D008875:Middle Aged; D009185:Myelin Proteins / Q000235:genetics*; D000070798:Nogo Proteins; D011110:Polymorphism, Genetic
D020413:3' Untranslated Regions; D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603199:RTN4 protein, human