[Influence of CRX gene on development of photoreceptors in zebrafish].
OBJECTIVE
To study the development and opsin expression of zebrafish photoreceptors after CRX gene knock-down.


METHODS
It was a experimental study. CRX-MOs was microinjected into the zygote of zebrafish. Seventy-two and 96 hours post fertilization, we prepared the eye histological slides, electron microscope samples and whole mount in-situ hybridizations to study the development and opsin expression. of photoreceptors.


RESULTS
There were no obvious histological changes in optical microscope observation after CRX-MOs microinjection. Outer segments were developed in control and blank groups, but no obvious outer segment was found in CRX group by electron microscope observation. Opsin Rho, SWS1 and SWS2 were expressed in control and blank groups and the expression of these genes was obviously decreased in CRX group.


CONCLUSIONS
CRX gene can influence the development and opsins expression of photoreceptors outer segments in zebrafish.
Zhang
Yan-qiong
YQ
Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai 200031, China. yanqiongzhang401@yahoo.com.cn
Wang
Yue-xiang
YX
Xu
Ge-zhi
GZ
Song
Hou-yan
HY
Wang
Wen-ji
WJ
D000818:Animals; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000235:genetics*; D010786:Photoreceptor Cells / Q000166:cytology*; D020419:Photoreceptor Cells, Vertebrate; D015534:Trans-Activators / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics
D018398:Homeodomain Proteins; D015534:Trans-Activators; C109288:cone rod homeobox protein