Chemical probes for profiling fatty acid-associated proteins in living cells.
Chemical probes appended with reactive electrophiles afford powerful tools for profiling discrete protein families in living cells. Herein, we have synthesized cell-permeable chemical probes that target fatty acid-associated proteins. These fatty acid-based chemical probes contain acyloxymethylketone or fluorophosphonate functional groups and an alkyne click chemistry tag for visualization of covalently modified proteins by in-gel fluorescence scanning. Our fatty acid-based chemical probe affords new tools to evaluate the activity/expression of lipid-associated proteins that should facilitate their functional characterization and inhibitor discovery.
Raghavan
Anuradha
A
The Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, 1230 York Avenue, Box 250, New York, NY 10065, USA.
Charron
Guillaume
G
Flexner
James
J
Hang
Howard C
HC
D000818:Animals; D002584:Cervix Uteri / Q000166:cytology / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000187:drug effects*; D005227:Fatty Acids / Q000032:analysis / Q000138:chemical synthesis / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009000:Monocytes / Q000378:metabolism; D054510:Phospholipases A2, Cytosolic / Q000378:metabolism
D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D015335:Molecular Probes; D054510:Phospholipases A2, Cytosolic