Production & characterization of a unique dextran from an indigenous Leuconostoc mesenteroides CMG713.
On the basis of high enzyme activity a newly isolated strain of L. mesenteroides CMG713 was selected for dextran production. For maximum yield of dextran, effects of various parameters such as pH, temperature, sucrose concentration and incubation period were studied. L. mesenteroides CMG713 produced maximum dextran after 20 hours of incubation at 30 masculineC with 15% sucrose at pH 7.0. The molecular mass distribution of dextran produced by this strain showed that its molecular mass was about 2.0 million Da. Dextran analysis by (13)C-NMR spectrometry showed no signals corresponding to any other linkages except alpha-(1-->6) glycosidic linkage in the main chain, which has not been reported before. Physico-chemical properties of this unique dextran were also studied. These optimised conditions could be used for the commercial production of this unique high molecular weight dextran, which have significant industrial perspectives.
Sarwat
Farwa
F
Centre for Molecular Genetics, University of Karachi, Pakistan.
Ul Qader
Shah Ali
SA
Aman
Afsheen
A
Ahmed
Nuzhat
N
D001431:Bacteriological Techniques; D003911:Dextrans / Q000096:biosynthesis* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007934:Leuconostoc / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D013395:Sucrose / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D003911:Dextrans; D013395:Sucrose