Effects of the fruit essential oil of Cuminum cyminum L. on the acquisition and expression of morphine-induced conditioned place preference in mice.
Rewarding properties of opioids are now accepted and widely discussed. These properties can lead to long-term usage of these substances. The main purpose of this study was to investigate the effects of Cuminum cyminum fruit essential oil (FEO) on the acquisition and expression of morphine-induced conditioned place preference (CPP) in mice. CPP was induced by subcutaneous (s.c.) injection of morphine (5mg/kg) in 3 days conditioning schedule. Intraperitoneal (i.p.) administration of Cumin FEO (0.001%, 0.01%, 0.1%, 0.5%, 1% and 2%; 5 ml/kg) or Tween-80 (0.5%; 5 ml/kg) did not show any conditioning effects. Administration of Cumin FEO (0.001-2%; 5 ml/kg; i.p.), 60 min before test on day 5 (expression) decreased the conditioning scores at the doses of 1% and 2% while i.p. injection of Cumin FEO (0.001-2%; 5 ml/kg), 60 min before morphine injection (5mg/kg; s.c.) during 3 days of conditioning session (acquisition) significantly resulted in decrement of rewarding properties of morphine at the doses of 0.1%, 0.5%, 1% and 2% in dose-dependent manner. Tween-80 as a vehicle did not suppress the acquisition and expression of morphine-induced CPP. The results showed that the C. cyminum fruit essential oil reduces the acquisition and expression of morphine-induced conditioned place preference in mice.
Khatibi
Ali
A
Neuroscience Research Center, Shahid Beheshti University, M.C., P.O. Box 19615-1178, Tehran, Iran.
Haghparast
Abbas
A
Shams
Jamal
J
Dianati
Elham
E
Komaki
Alireza
A
Kamalinejad
Mohammad
M
D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D003216:Conditioning, Operant / Q000187:drug effects*; D028527:Cuminum / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry; D008297:Male; D051379:Mice; D009020:Morphine / Q000008:administration & dosage*; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D009294:Narcotics / Q000008:administration & dosage*; D009822:Oils, Volatile / Q000494:pharmacology*; D012054:Reinforcement, Psychology; D013997:Time Factors
D009294:Narcotics; D009822:Oils, Volatile; D009020:Morphine