title: Influence of inflammation and aging on macrophage inhibitory cytokine-1 gene expression in rat ventral prostate.
abstract: OBJECTIVE
  We have previously reported that the macrophage inhibitory cytokine-1 (MIC-1) gene is downregulated in human symptomatic benign prostatic hyperplasia. The aim of this study was to investigate the histologic changes and MIC-1 gene expression in the prostate of young nonbacterial prostatitis model (Y-NBP) and aging rats.
  
  
  METHODS
  A total of 35 Wistar male rats, 13 weeks old, were castrated and subjected to (a) castration alone for 14 days, (b) Y-NBP-14d (0.25 mg/2 mL/kg beta-estradiol injection for 14 days), or (c) Y-NBP-30d (beta-estradiol injection for 30 days). A total of 5 male rats, 10 months old, were also analyzed. We used 21 male rats, 13 weeks old, who had undergone sham surgery as the controls. The ventral lobes of the prostate were histologically examined with Masson's trichrome staining or immunostaining using an anti-macrophage antibody. The MIC-1 mRNA levels were quantitatively assessed using real-time reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  The MIC-1 gene mRNA levels in the castration alone, Y-NBP-14d, and Y-NBP-30d rat prostates were greater than those in the control rats (P < .005). In contrast, those of the 10-month-old rats were lower than those of the controls (P = .0093). The mean stroma-to-epithelium ratio in the Y-NBP-30d rats, 10-month-old rats, and 13-week-old controls was 1.28, 0.26, and 0.10, respectively (Y-NBP-30d vs 10-month-old rats, P = .0008; 10-month-old vs 13-week-old rats, P = .001). The number of infiltrating macrophages in the Y-NBP-14d, Y-NBP-30d, and 10-month-old rats was greater than that of the 13-week-old controls (P < .001).
  
  
  CONCLUSIONS
  Castration causes induction of MIC-1 gene expression. Estradiol treatment has little effect on MIC-1 gene expression but causes a significant increase in the stroma-to-epithelium ratio. The aging rat prostate is more similar to human benign prostatic hyperplasia than is the Y-NBP model in light of MIC-1 gene expression and histologic changes.
authors.affiliation: Department of Urology, Kagawa University Faculty of Medicine, Kita-gun, Kagawa, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Taniguchi
authors.forename: Rikiya
authors.initials: R
authors.lastname: Taoka
authors.forename: Masashi
authors.initials: M
authors.lastname: Inui
authors.forename: Mikio
authors.initials: M
authors.lastname: Sugimoto
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kakehi
mesh_terms: D000367:Age Factors; D000818:Animals; D015870:Gene Expression; D055436:Growth Differentiation Factor 15 / Q000235:genetics*; D008297:Male; D011472:Prostatitis / Q000235:genetics* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D055436:Growth Differentiation Factor 15