The effect of fecal turbidity on norovirus detection by reverse transcriptase polymerase chain reaction.
A 10-min fecal preparation results in greater specimen turbidity than a 45-min protocol, but reverse transcriptase polymerase chain reaction (RT-PCR) norovirus test sensitivity is essentially the same. Feces processed so that particle size does not exceed approximately 560 nm do not display greater norovirus RT-PCR inhibitory effects than those that have undergone greater purification.
Witlox
Kristie J
KJ
Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia.
Karapanagiotidis
Theo
T
Bruggink
Leesa D
LD
Marshall
John A
JA
D005243:Feces / Q000821:virology*; D006801:Humans; D029322:Norovirus / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors