title: High-throughput assays probing protein-RNA interactions of eukaryotic translation initiation factors.
abstract: Protein-RNA interactions are involved in all facets of RNA biology. The identification of small molecules that selectively block such bimolecular interactions could provide insight into previously unexplored steps of gene regulation. Such is the case for regulation of eukaryotic protein synthesis where interactions between messenger RNA (mRNA) and several eukaryotic initiation factors govern the recruitment of 40S ribosomes (and associated factors) to mRNA templates during the initiation phase. We have designed simple fluorescence polarization-based high-throughput screening assays that query the binding of several translation factors to RNA and found that the mixed inhibitor p-chloromercuribenzoate interferes with poly(A) binding protein-RNA interaction.
authors.affiliation: Department of Biochemistry, McGill University, Montreal, Que., Canada H3G1Y6.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Galicia-Vázquez
authors.forename: Lisa
authors.initials: L
authors.lastname: Lindqvist
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.forename: Isabelle
authors.initials: I
authors.lastname: Harvey
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Jerry
authors.initials: J
authors.lastname: Pelletier
mesh_terms: D039642:Eukaryotic Initiation Factors / Q000032:analysis / Q000378:metabolism*; D005454:Fluorescence Polarization / Q000379:methods*; D006801:Humans; D039102:Poly(A)-Binding Proteins / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000378:metabolism*; D020245:p-Chloromercuribenzoic Acid / Q000737:chemistry
chemical_list: D039642:Eukaryotic Initiation Factors; D039102:Poly(A)-Binding Proteins; D020245:p-Chloromercuribenzoic Acid; D012313:RNA