Efficient post-exposure prophylaxis against rabies by applying a four-dose DNA vaccine intranasally.
We tested two post-exposure prophylaxes (PEPs) for rabies in laboratory animals; one was a traditional antirabies vaccine for humans via intramuscular route (IM), and the other was a DNA vaccine administered by intranasal route (IN). In contrast to The World Health Organization's recommended five-dose PEP, we gave only four doses without hyper-immune antirabies sera, making the PEP more rigorous. All animals were challenged with challenge virus strain (CVS); 16h later, PEP was applied. All animals that received the PEP with DNA/IN survived, and 87% of the rabbits and 80% of the mice that received the PEP with traditional antirabies vaccine/IM survived. Negative controls succumbed to infection. The expression of G protein was detected in the NALT, cerebellum, cerebral cortex (neocortex), cerebellum and hippocampus, mainly in the glial cells (microglia) and microvessels. On the other hand, plasmid construct was detected in brain and its mRNA expression in medium and posterior encephalon. The efficiency of this DNA/IN PEP is probably due to the early expression of the antigen in the brain stimulating the immune system locally.
Tesoro Cruz
Emiliano
E
Departamento de Investigación Experimental y Bioterio del Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico.
Feria Romero
Iris Angélica
IA
López Mendoza
Juan Gabriel
JG
Orozco Suárez
Sandra
S
Hernández González
Rafael
R
Favela
Francisco Blanco
FB
Pérez Torres
Armando
A
José Alvaro Aguilar Setién
D000281:Administration, Intranasal; D000818:Animals; D001921:Brain / Q000276:immunology / Q000473:pathology / Q000821:virology; D002460:Cell Line; D004396:Coloring Agents; D006224:Cricetinae; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004801:Eosine Yellowish-(YS); D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics / Q000276:immunology; D006416:Hematoxylin; D007115:Immunization Schedule; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010957:Plasmids / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D011818:Rabies / Q000517:prevention & control*; D011819:Rabies Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D011820:Rabies virus / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use
D004396:Coloring Agents; D018076:DNA, Complementary; D012367:RNA, Viral; D011819:Rabies Vaccines; D019444:Vaccines, DNA; D019204:GTP-Binding Proteins; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin