Establishment of a new glial cell line from hippocampus of prion protein gene-deficient mice.
Cellular prion protein (PrP(C)) is expressed not only in neuronal cells but also in non-neuronal cells such as astroglial cells. In the present study, the prion protein (PrP) gene (Prnp)-deficient astroglial cell line GpL1 from hippocampal cells of ZrchI Prnp(-/-) mice were established. Transfection of Prnp suppressed cell death in GpL1 cells under serum-free conditions. The PrP-expressing GpL1 cells showed increased superoxide dismutase activity compared to control GpL1 cells. These results suggest that the anti-oxidative activity of PrP(C) functions in not only neuronal cells but also astroglial cells possibly due to the increased anti-oxidative activity of astroglial cells.
Nishimura
Takuya
T
Department of Molecular Immunology, School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Sakudo
Akikazu
A
Xue
Guangai
G
Ikuta
Kazuyoshi
K
Yukawa
Masayoshi
M
Sugiura
Katsuaki
K
Onodera
Takashi
T
D000818:Animals; D002460:Cell Line; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D000072002:Prion Proteins; D011328:Prions / Q000235:genetics* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014162:Transfection
D000072002:Prion Proteins; D011328:Prions; C512657:Prnp protein, mouse; D013482:Superoxide Dismutase