Application of rhamnolipid and surfactin for enhanced diesel biodegradation--effects of pH and ammonium addition.
This study investigated the effects of pH and ammonium concentrations on the potential application of two biosurfactants, surfactin (SF) and rhamnolipid (RL), for enhanced diesel biodegradation with a series of bench-scale experiments. In general, compared to the experiments without biosurfactant addition, adding RL or SF to diesel-water systems at concentrations above their critical micelle concentration (CMC) values benefited diesel emulsification, and therefore enhanced diesel biodegradation. The effects of pH on RL or SF-enhanced biodegradation of diesel were in good agreement with the trends of emulsion index values for RL or SF addition, respectively, under different pH conditions, suggesting that enhanced diesel emulsification by RL or SF addition promoted biodegradation of diesel. In diesel-water systems with 50mg/L of RL addition, an optimum pH condition for microbial growth and diesel biodegradation was found to be at a pH 7.2, while decreasing pH to 5.2 or increasing it to 8.4 reduced those parameters considerably. For the cases where 40 mg/L of SF was added, the enhancing ability shared a general trend with that observed for adding 50mg/L of RL as the pH increased from 5.2 to 7.2. Further increase of pH to 8.4, however, did not seem to negatively influence biodegradation and biomass growth. With respect to the effects of ammonium concentration on diesel biodegradation in diesel-water systems with 50mg/L of RL addition, an optimum ammonium addition for microbial growth and diesel biodegradation was found between 200 and 300 mg-N/L, but a dramatic decrease in growth and biodegradation occurred at ammonium addition up to 450 mg-N/L. For the cases where 40 mg/L of SF was added, an increase of ammonium addition from 50 to 200mg-N/L substantially increased microbial growth and biodegradation of diesel. Further increase of ammonium concentration to 450 mg-N/L, however, did not further improve diesel biodegradation.
Whang
Liang-Ming
LM
Department of Environmental Engineering, National Cheng Kung University, Tainan, Taiwan, ROC. whang@mail.ncku.edu.tw
Liu
Pao-Wen G
PW
Ma
Chih-Chung
CC
Cheng
Sheng-Shung
SS
D001419:Bacteria / Q000254:growth & development / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D005742:Gasoline / Q000382:microbiology*; D006017:Glycolipids; D006863:Hydrogen-Ion Concentration; D055666:Lipopeptides; D010456:Peptides, Cyclic; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents
D005742:Gasoline; D006017:Glycolipids; D055666:Lipopeptides; D010456:Peptides, Cyclic; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; C418382:rhamnolipid; C009365:surfactin peptide