[The influence of degenerative changes on the production of free radicals and the lipid peroxidation at the patients after alloplasty of the hip joint].
Since now researches claimed that rheumatoid arthritis was not characterized with an inflammatory process due to the lack of vessel in the cartilaginous tissue. Nevertheless, an inflammation of synovial membrane and a reduction of activity of articulation co-exist with rheumatoid arthritis. Recent results have proved that the above disease is connected with an oxidative stress.


OBJECTIVE
The aim of this investigation was to estimate changes of nitric oxide (NO) in plasma and malondialdehyd (MDA) concentration in red blood cells of patients suffering from an alloplastic of the hip joint. We also measured how physical activity in a reduced motor activity influences the NO and MDA concentration.


METHODS
Malonyl dialdehyde (MDA) concentration in RBCs was assayed with Placer method, nitrogen concentration in blood plasma was determined using the Griess indirect method. Biochemical tests have been performed on a group of 36 patients with osteoarthritis hospitalised at the Traumatic-Orthopaedic Department of the Ministry of Internal Affairs and Administration Hospital in Lodz, Poland. A control group included 21 subjects with normal physical activity.


RESULTS
The concentration of nitric oxide in plasma of patients that were operated was lower than in a healthy control group. Ten days after the operation it decreased, but after a month it was higher than before the operation. The concentration of MDA in red blood cells was higher before and after the alloplastic than in the healthy control group. Ten days after operation the concentration of MDA was lower about 15.8% but after 30 days it lowered up to about 26.3% in comparison to the concentration before the operation.


CONCLUSIONS
Our results are not the same as those shown by other researches which suggest increased production of nitric oxide. Reduction of the motor activity due to degenerative joint disease and alloplastic causes reduction in lipid peroxidation of red blood cells.
Mrowicka
Małgorzata
M
Uniwersytet Medyczny w Łodzi, Wydział Fizjoterapii, Katedra Chemii i Biochemii Klinicznej, Zakład Chemii i Biochemii Klinicznej. mrowmal@o2.pl
Gałecka
Elzbieta
E
Miller
Elzbieta
E
Garncarek
Paweł
P
D000368:Aged; D015415:Biomarkers / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005609:Free Radicals / Q000097:blood*; D006622:Hip Prosthesis / Q000009:adverse effects*; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D009569:Nitric Oxide / Q000097:blood*; D015207:Osteoarthritis, Hip / Q000209:etiology* / Q000378:metabolism*; D018384:Oxidative Stress; D013583:Synovial Membrane / Q000378:metabolism; D013585:Synovitis / Q000209:etiology* / Q000378:metabolism
D015415:Biomarkers; D005609:Free Radicals; D009569:Nitric Oxide; D008315:Malondialdehyde