Tissue estimation of protein gene product 9.5 (PGP 9.5) expression and apoptosis in vitiligo.
BACKGROUND
Vitiligo is probably the end result of different interacting processes.


OBJECTIVE
To determine the possible roles of neural and apoptotic mechanisms in the pathogenesis of vitiligo.


METHODS
Fifty-six biopsies from 28 patients with generalized vitiligo (28 from depigmented lesional areas and 28 from clinically nondepigmented skin at the periphery of the same areas) were examined; the panaxonal marker neuropeptide protein gene product 9.5 (PGP 9.5) and apoptosis were investigated using immunohistochemistry.


RESULTS
Statistically significant differences were detected in the numbers of PGP 9.5-positive nerve fibers/axons in the papillary dermis between the center and periphery of the lesions (i.e. increased at the center in comparison with the periphery). A statistically significant inverse association was found between PGP 9.5 immunostaining in the dermis at the lesion center and the duration of the disease. When apoptosis and PGP 9.5 expression were compared, there was an identical distribution of PGP 9.5-positive nerve fibers/axons and apoptotic cells in the epidermis (i.e. basal in the lesion center; diffuse at the lesion periphery).


CONCLUSIONS
There is a possible connection between the neural and apoptotic pathogenetic theories of vitiligo.
Aroni
Kyriaki
K
Dermatopathology Section, 1st Department of Pathology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Grapsa
Antonia
A
Lazaris
Andreas C
AC
Kavantzas
Nikolaos
N
Kordosis
Theodoros
T
Patsouris
Efstratios S
ES
D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics* / Q000502:physiology; D001707:Biopsy, Needle; D015331:Cohort Studies; D005260:Female; D005786:Gene Expression Regulation; D005819:Genetic Markers; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012307:Risk Factors; D012680:Sensitivity and Specificity; D014820:Vitiligo / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology; D055815:Young Adult
D005819:Genetic Markers; D011506:Proteins