In vitro fertilization, embryo development, and cell lineage segregation after pre- and/or postnatal exposure of female mice to ambient fine particulate matter.
OBJECTIVE
To evaluate effects of pre- and/or postnatal exposure to ambient fine particulate matter on fertilization, embryo development, and cell lineage segregation in preimplantation blastocysts using the IVF mouse model.


METHODS
Animal model.


METHODS
Academic institution.


METHODS
Six-week-old, superovulated mice.


METHODS
Pre- and postnatal exposure to filtered air (FA-FA), filtered-ambient air (FA-AA), or ambient air (AA-AA) in exposure chambers 24 hours a day for 9 weeks.


METHODS
Gestation length, litter size, sex ratio, ovarian response to superovulation, fertilization rate, embryo development, blastocyst and hatching rates, total cell count, and proportion of cell allocation to inner-cell mass (ICM) and trophectoderm (TE).


RESULTS
Gestation length, litter size and birth weight, live-birth index, and sex ratio were similar among exposure groups. Ovarian response was not affected by the exposure protocol. A multivariate effect for pre- and/or postnatal exposure to ambient fine particulate matter on IVF, embryo development, and blastocyst differential staining was found. Cell counts in ICM and ICM/TE ratios in blastocysts produced in the FA-FA protocol were significantly higher than in blastocysts produced in the FA-AA and AA-AA protocols. No difference in total cell count was observed among groups.


CONCLUSIONS
Our study suggests that exposure to ambient fine particulate matter may negatively affect female reproductive health by disrupting the lineage specification at the blastocyst stage without interfering in early development of the mouse embryo.
Maluf
Mariangela
M
Department of Pathology, University of São Paulo School of Medicine, São Paulo, Brazil. mariangela@ceerh.com.br
Perin
Paulo Marcelo
PM
Foltran Januário
Daniela Aparecida Nicolosi
DA
Nascimento Saldiva
Paulo Hilário
PH
D000818:Animals; D019070:Cell Lineage / Q000187:drug effects*; D002478:Cells, Cultured; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D019570:Inhalation Exposure / Q000009:adverse effects; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects; D051379:Mice; D010062:Ovulation Induction; D010316:Particle Size; D052638:Particulate Matter / Q000494:pharmacology* / Q000633:toxicity; D011247:Pregnancy; D018873:Pregnancy Rate; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000503:physiopathology; D012098:Reproduction / Q000187:drug effects
D052638:Particulate Matter