Evaluation of mRNA by Q-RTPCR and protein expression by AQUA of the M2 subunit of ribonucleotide reductase (RRM2) in human tumors.
OBJECTIVE
The purpose of this study was to evaluate baseline RRM2 protein and gene expression in tumors of patients receiving 3-AP.


METHODS
Tumor blocks from patients enrolled in phase I and II clinical studies using 3-AP, were evaluated for RRM2 gene and protein expression by quantitative real time polymerase chain reaction (Q-RTPCR) and automated quantitative analysis (AQUA).


RESULTS
Esophageal and gastric cancers overexpressed RRM2 protein when compared to prostate cancer (Z-score, 0.68 +/- 0.94 SD, vs 0.41 +/- 0.84 SD, respectively; p = 0.04). Esophageal and gastric cancers also overexpressed RRM2 mRNA when compared to prostate cancer (relative gene expression 2.56 +/- 1.49 SD, vs 0.29 +/- 0.20 SD, respectively; p = 0.02). Protein and gene expression were moderately associated (Spearman's rank correlation = 0.30; p = 0.12).


CONCLUSIONS
RRM2 gene and protein expression varies by tumor type.
Kolesar
Jill
J
University of Wisconsin Paul P Carbone Comprehensive Cancer Center, Madison, WI 53792, USA. jmkolesar@pharmacy.wisc.edu
Huang
Wei
W
Eickhoff
Jens
J
Hahn
Kristine
K
Alberti
Dona
D
Attia
Steven
S
Schelman
William
W
Holen
Kyle
K
Traynor
Anne
A
Ivy
Percy
P
Wilding
George
G
D017321:Clinical Trials, Phase I as Topic; D017322:Clinical Trials, Phase II as Topic; D004938:Esophageal Neoplasms / Q000188:drug therapy / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000235:genetics; D021122:Protein Subunits; D011725:Pyridines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D012262:Ribonucleoside Diphosphate Reductase / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics; D013882:Thiosemicarbazones / Q000494:pharmacology*
D021122:Protein Subunits; D011725:Pyridines; D012333:RNA, Messenger; D013882:Thiosemicarbazones; C078157:3-aminopyridine-2-carboxaldehyde thiosemicarbazone; C093713:ribonucleotide reductase M2; D012262:Ribonucleoside Diphosphate Reductase