Colony multiplex-tandem PCR for rapid, accurate identification of fungal cultures.
We developed a multiplex tandem PCR (MT-PCR) assay for the rapid identification of 16 fungi directly from culture. MT-PCR results were concordant with phenotypic identification for all cultures studied (n = 183). The colony MT-PCR assay was rapid (<2 h), sensitive, and specific in identifying fungal pathogens directly from primary isolation plates.
Lau
Anna
A
Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, New South Wales, Australia.
Sorrell
Tania C
TC
Lee
OkCha
O
Stanley
Keith
K
Halliday
Catriona
C
D005658:Fungi / Q000145:classification* / Q000302:isolation & purification*; D006801:Humans; D009181:Mycoses / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity