title: Myogenic origin of the hypotension induced by rapid changes in posture in awake dogs following autonomic blockade.
abstract: The "push-pull" effect denotes the reduced tolerance to +G(z) (hypergravity) when +G(z) stress is preceded by exposure to hypogravity, i.e., fractional, zero, or negative G(z). The purpose of this study was to test the hypothesis that an exaggerated, myogenically mediated rise in leg vascular conductance contributes to the push-pull effect, using heart level arterial blood pressure as a measure of G tolerance. The approach was to impose control (30 s of 30 degrees head-up tilt) and push-pull (30 s of 30 degrees head-up tilt immediately preceded by 10 s of -15 degrees head-down tilt) gravitational stress after administration of hexamethonium (5 mg/kg) to inhibit autonomic ganglionic neurotransmission in seven dogs. Cardiac output or thigh level arterial pressure (myogenic stimulus) was maintained constant by computer-controlled ventricular pacing. The animals were sedated with acepromazine and lightly restrained in lateral recumbency on a tilt table. Following the onset of head-up tilt, the magnitude of the fall in heart level arterial pressure from baseline was -11.6 +/- 2.9 and -17.1 +/- 2.2 mmHg for the control and push-pull trials, respectively (P < 0.05), when cardiac output was maintained constant. Over 40% of the exaggerated fall in heart level arterial pressure was attributable to an exaggerated rise in hindlimb vascular conductance (P < 0.05). Maintaining thigh level arterial pressure constant abolished the exaggerated rise in hindlimb blood flow. Thus a push-pull effect largely attributable to a myogenically induced rise in leg vascular conductance occurs when autonomic function is inhibited.
authors.affiliation: Integrative Physiology, 424 Field House, Iowa City, IA 52242, USA.
authors.forename: Brett J
authors.initials: BJ
authors.lastname: Wong
authors.forename: Don D
authors.initials: DD
authors.lastname: Sheriff
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000502:physiology*; D002302:Cardiac Output / Q000502:physiology; D003627:Data Interpretation, Statistical; D004285:Dogs; D006112:Gravitation; D018475:Head-Down Tilt; D006439:Hemodynamics / Q000502:physiology; D006614:Hindlimb / Q000098:blood supply / Q000502:physiology; D007022:Hypotension / Q000139:chemically induced / Q000503:physiopathology*; D018482:Muscle, Skeletal / Q000503:physiopathology*; D019148:Neuromuscular Blockade; D011187:Posture / Q000502:physiology*; D012039:Regional Blood Flow / Q000502:physiology