[Effects on lymphangiogenesis and angiogenesis of lung cancer with antisense oligonucleotides of vascular endothelial growth factor C].
OBJECTIVE
To investigate the effects on lymphangiogenesis and angiogenesis of orthotopic implantation of lung cancer in nude mice with antisense oligonucleotides of VEGF-C.


METHODS
The model in nude mice was established with orthotopic implantation for the human lung cancer cell line A549. Thirty nude mice were randomized into three groups: PBS control group, sense oligonucleotides control group and antisense oligonucleotides group (AODN group). After treatments were completed, the expression of VEGF-C and lymphatic microvessel density (LMVD) and microvessel density (MVD) of lung cancer were detected by RT-PCR,Western Blot and immunohistochemistry.


RESULTS
The expression of VEGF-C in AODN group was inhibit significantly (P < 0.05). The LMVD in AODN group was decreased significantly (P < 0.1). Though the MVD in AODN group was also decreased, but there were no significant differences compared with control groups (P > 0.05).


CONCLUSIONS
The antisense oligonucleotides of VEGF-C can inhibit the expression of VEGF-C in nude mice of orthotopic implantation of lung cancer. It could inhibit the lymphangiogenesis.
Guo
Xu-Feng
XF
Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China shandagxf@126.com
Xu
Zhong-Hua
ZH
Chen
Yong-Bing
YB
Xu
Zhong-Heng
ZH
Yang
Wen-Tao
WT
Qian
Yong-Yue
YY
Hu
Guo-Qiang
GQ
D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008081:Liposomes; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D042583:Lymphangiogenesis / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D055806:Microvessels / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011897:Random Allocation; D014162:Transfection; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
D008081:Liposomes; D016376:Oligonucleotides, Antisense; D042582:Vascular Endothelial Growth Factor C