MmeI: a minimal Type II restriction-modification system that only modifies one DNA strand for host protection.
MmeI is an unusual Type II restriction enzyme that is useful for generating long sequence tags. We have cloned the MmeI restriction-modification (R-M) system and found it to consist of a single protein having both endonuclease and DNA methyltransferase activities. The protein comprises an amino-terminal endonuclease domain, a central DNA methyltransferase domain and C-terminal DNA recognition domain. The endonuclease cuts the two DNA strands at one site simultaneously, with enzyme bound at two sites interacting to accomplish scission. Cleavage occurs more rapidly than methyl transfer on unmodified DNA. MmeI modifies only the adenine in the top strand, 5'-TCCRAC-3'. MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5'-GTYGGA-3'. There is no additional DNA modification associated with the MmeI R-M system, as is required for previously characterized Type IIG R-M systems. The MmeI R-M system thus uses modification on only one of the two DNA strands for host protection. The MmeI architecture represents a minimal approach to assembling a restriction-modification system wherein a single DNA recognition domain targets both the endonuclease and DNA methyltransferase activities.
Morgan
Richard D
RD
New England Biolabs Inc., Ipswich, MA and MCB Department, Brown University, Providence, RI, USA.
Bhatia
Tanya K
TK
Lovasco
Lindsay
L
Davis
Theodore B
TB
D000225:Adenine / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D053837:DNA Cleavage; D019175:DNA Methylation; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020630:Methylophilus methylotrophus / Q000235:genetics; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C049760:endodeoxyribonuclease MmeI; D015252:Deoxyribonucleases, Type II Site-Specific; D000225:Adenine