title: High contrast in vitro and in vivo photoluminescence bioimaging using near infrared to near infrared up-conversion in Tm3+ and Yb3+ doped fluoride nanophosphors.
abstract: A new approach for photoluminescence imaging in vitro and in vivo has been shown utilizing near infrared to near infrared (NIR-to-NIR) up-conversion in nanophosphors. This NIR-to-NIR up-conversion process provides deeper light penetration into biological specimen and results in high contrast optical imaging due to absence of an autofluorescence background and decreased light scattering. Aqueous dispersible fluoride (NaYF4) nanocrystals (20-30 nm size) co-doped with the rare earth ions, Tm(3+) and Yb(3+), were synthesized and characterized by TEM, XRD, and photoluminescence (PL) spectroscopy. In vitro cellular uptake was shown by the PL microscopy visualizing the characteristic emission of Tm(3+) at approximately 800 nm excited with 975 nm. No apparent cytotoxicity was observed. Subsequent animal imaging studies were performed using Balb-c mice injected intravenously with up-converting nanophosphors, demonstrating the high contrast PL imaging in vivo.
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, Department of Chemistry, University at Buffalo, State University of New York, Buffalo, New York 14260-3000, USA.
authors.forename: Marcin
authors.initials: M
authors.lastname: Nyk
authors.forename: Rajiv
authors.initials: R
authors.lastname: Kumar
authors.forename: Tymish Y
authors.initials: TY
authors.lastname: Ohulchanskyy
authors.forename: Earl J
authors.initials: EJ
authors.lastname: Bergey
authors.forename: Paras N
authors.initials: PN
authors.lastname: Prasad
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002412:Cations / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival; D005459:Fluorides / Q000737:chemistry*; D008163:Luminescent Measurements; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010777:Photochemistry; D013932:Thulium / Q000737:chemistry*; D014961:X-Ray Diffraction; D015018:Ytterbium / Q000737:chemistry*
chemical_list: D002412:Cations; D013932:Thulium; D015018:Ytterbium; D005459:Fluorides