A novel approach of prophylaxis to HBV recurrence after liver transplantation.
Liver transplantation (LT) in patients with hepatitis B virus (HBV) infection is associated with a high rate of graft loss and poor survival, unless re-infection can be prevented. Human hepatitis B immune globulin (HBIG) and nucleoside analogues (NA) have long been utilized to prevent re-infection. Previously, we generated a human monoclonal antibody (mAb), HB that recognizes the surface antigen of hepatitis B virus (HBV). We have constructed a secreted version of HB and cloned its genes into recombinant adeno-associated virus (AAV). We compared the efficiency of AAV vector after a single injection via intramuscular or intravenous routes without additional intervention. Then, we evaluated the activity of antibody HB in tree shrews treated with rAAV-HB and in vitro experiments. So, intramuscular injection of rAAV-HB was a suitable method for the immunoprophylaxis of HBV infection. This human antibody will be useful for the immunoprophylaxis of HBV infection.
Pan
Tao
T
Key Laboratory of Organ Transplantation, Ministry of Education/Ministry of Public Health, Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Cai
Ming
M
Tang
Li
L
Zhou
Li Qiao
LQ
Li
Bin Jian
BJ
Zhu
Tong
T
Li
Hong Zhou
HZ
Li
Shu Yuan
SY
Xiao
Xiao
X
Chen
Zhi Shui
ZS
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000627:therapeutic use*; D001483:Base Sequence; D018890:Chemoprevention / Q000379:methods*; D000229:Dependovirus / Q000235:genetics; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006509:Hepatitis B / Q000517:prevention & control*; D006510:Hepatitis B Antibodies / Q000235:genetics / Q000627:therapeutic use*; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D016031:Liver Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D055502:Secondary Prevention; D014418:Tupaiidae
D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies