The conformational properties of methyl alpha-(2,8)-di/trisialosides and their N-acyl analogues: implications for anti-Neisseria meningitidis B vaccine design.
The conformational properties of di- and trisaccharide fragments of the polysialic acid O-antigen capsular polysaccharide (CPS) of Neisseria meningitidis B (NmB) have been investigated by a combination of solution phase NMR spectroscopy and explicit-solvent molecular dynamics (MD) simulations. Simulations employing 100 ns of conventional MD, as well as 160 ns of replica exchange MD (REMD), with the GLYCAM06 force field were shown to be in agreement with experimental NMR scalar J-coupling and NOE values. The presence of conformational families has been determined by monitoring interglycosidic torsion angles, by comparing structural superimpositions, as well as via a Bayesian statistical analysis of the torsional data. Attempts to augment the immunogenicity of NmB CPS often involve chemical modifications of the N-acetyl moiety. Here the effects of these chemical group modifications on the conformational properties of the trisialoside have been probed via REMD simulations of the N-glycolyl, N-propionyl, N-propyl and N-butanoyl analogues. Although there were conformational families unique to each non-native analogue, the chemical modifications resulted in largely equivalent overall conformational phase-spaces compared to the native trisialoside. On the basis of the conformational distributions, these shared conformational properties suggest that a recurrent global conformational epitope may be present in both the native and chemically modified CPS fragments. Explanations are therefore provided for monoclonal antibody cross-reactivity, in terms of recognition of a shared global CPS conformation, as well as for lack of cross-reactivity, in terms of fine structural differences associated with the N-acyl groups, which may be dominant in highly matured antibody responses.
Yongye
Austin B
AB
Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, Georgia 30602, USA.
Gonzalez-Outeiriño
Jorge
J
Glushka
John
J
Schultheis
Verena
V
Woods
Robert J
RJ
D000907:Antibodies, Bacterial / Q000276:immunology; D016667:Bacterial Capsules / Q000737:chemistry / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D002236:Carbohydrate Conformation; D003429:Cross Reactions; D015195:Drug Design; D006241:Haptens / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D038541:Neisseria meningitidis, Serogroup B / Q000276:immunology*; D012794:Sialic Acids / Q000737:chemistry*
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D006241:Haptens; D012794:Sialic Acids