Searching biomarker candidates in serum using multidimensional native chromatography. II Method evaluation with Alport syndrome and severe inflammation.
Biomarker search using multidimensional native liquid fractionation of serum in microplates was evaluated. From different donors, homologous sample fractions with UV absorbance depending on state of illness were selected, and their constituents were identified and quantitated by MS. Analysis of sera of patients with Alport syndrome and severe inflammation proved the reliability of the method by confirming characteristic alterations. Moreover, 23 new marker candidates were detected for Alport syndrome, some of them being involved in matrix degradation and repair, and 33 new candidates for severe inflammation, among them alpha1B-glycoprotein cysteine-rich secretory protein and an apparently low molecular-weight albumin variant.
Baum
Adrienne
A
Institute of Biochemistry, Friedrich Schiller University, Medical Faculty, Jena, Germany.
Pohl
Michael
M
Kreusch
Stefan
S
Cumme
Gerhard A
GA
Ditze
Günter
G
Misselwitz
Joachim
J
Kiehntopf
Michael
M
Udby
Lene
L
Meier-Hellmann
Andreas
A
Rhode
Heidrun
H
D000293:Adolescent; D015415:Biomarkers / Q000097:blood*; D005591:Chemical Fractionation / Q000379:methods; D002648:Child; D005260:Female; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D008297:Male; D009394:Nephritis, Hereditary / Q000097:blood*; D018805:Sepsis / Q000097:blood*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
C048566:A1BG protein, human; D015415:Biomarkers; D006023:Glycoproteins; D007136:Immunoglobulins