[Oxidative stress and endothelium dysfunction in diabetes mellitus type 2].
The aim of our study was to estimate the influence of oxidative stress on the function of vascular endothelium in diabetes mellitus type 2, through the measurement plasma levels of chosen parameters reflecting these disturbances--concentration of advanced oxidation protein products (AOPP) and activity of gamma glutamyltransferase (GGT) and N-acetyl-beta-glucosaminidase (NAG).


METHODS
73 patients with type 2 diabetes mellitus and 23 healthy people, which made up the control group were examined. In plasma of these subjects concentration of AOPP by spectrophotometric method with the use of potassium iodide; activity of GGT by spectrophotometric method and NAG activity by spectrofluorymetric method were determined.


RESULTS
The plasma levels of all examined parameters were significantly higher in diabetic patients than in healthy people. We observed increase of these parameters in groups of patients clustered on the basis of disease duration, glicemic state of diabetes and vascular area occupied by diabetic complication. The most significant changes in AOPP concentration were observed. There was statistically significant correlation between AOPP and GGT, however there was no any direct connection of these two parameters with NAG activity.


CONCLUSIONS
We showed significantly increase in the AOPP concentration and activities of GGT and NAG in plasma of patients with type 2 diabetes, which was induced by hyperglycemia and oxidative stress connected with this disease. The growth of examined parameters had no direct connection with each other, however was connected with chronic nature of this disease, glycemic decomposition and vascular area occupied by diabetic complication.
Piwowar
Agnieszka
A
Akademia Medyczna we Wrocdawiu, Katedra i Zakład Biochemii Farmaceutycznej. piwowar@biochfarm.am.wroc.pl
Knapik-Kordecka
Maria
M
Warwas
Maria
M
D000118:Acetylglucosaminidase / Q000097:blood*; D000328:Adult; D015415:Biomarkers / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D008875:Middle Aged; D018384:Oxidative Stress; D012016:Reference Values; D005723:gamma-Glutamyltransferase / Q000097:blood*
D015415:Biomarkers; D005723:gamma-Glutamyltransferase; D000118:Acetylglucosaminidase