title: Assessing mitochondrial morphology and dynamics using fluorescence wide-field microscopy and 3D image processing.
abstract: Mitochondrial morphology and length change during fission and fusion and mitochondrial movement varies dependent upon the cell type and the physiological conditions. Here, we describe fundamental wide-field fluorescence microscopy and 3D imaging techniques to assess mitochondrial shape, number and length in various cell types including cancer cell lines, motor neurons and astrocytes. Furthermore, we illustrate how to assess mitochondrial fission and fusion events by 3D time-lapse imaging and to calculate mitochondrial length and numbers as a function of time. These imaging methods provide useful tools to investigate mitochondrial dynamics in health, aging and disease.
authors.affiliation: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Song
authors.affiliation: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816.|The Burnham Institute for Medical Research, 10901 N. Torrey Pines Road, La Jolla, CA 92037.
authors.forename: Blaise
authors.initials: B
authors.lastname: Bossy
authors.affiliation: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816.
authors.forename: Ola J
authors.initials: OJ
authors.lastname: Martin
authors.affiliation: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816.|University of Pennsylvania, 3451 Walnut Street, Philadelphia, PA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Hicks
authors.affiliation: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816.
authors.forename: Sarah
authors.initials: S
authors.lastname: Lubitz
authors.affiliation: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816.
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Knott
authors.affiliation: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816.|The Burnham Institute for Medical Research, 10901 N. Torrey Pines Road, La Jolla, CA 92037.
authors.forename: Ella
authors.initials: E
authors.lastname: Bossy-Wetzel
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008928:Mitochondria / Q000502:physiology / Q000648:ultrastructure*; D053142:Mitochondrial Size; D009046:Motor Neurons / Q000648:ultrastructure; D051381:Rats