title: Masculinization of XX Drosophila transgenic flies expressing the Ceratitis capitata DoublesexM isoform.
abstract: The Doublesex (DSX) transcription factor regulates somatic sexual differentiation in Drosophila melanogaster. Female and male isoforms (DSXF and DSXM) are produced due to sex-specific RNA splicing. Here we show that in the distantly related dipteran Ceratitis capitata, the DSXM male-specific isoform is conserved and able to induce masculinization of both somatic and germline tissues when ectopically expressed in XX Drosophila transgenic individuals.
authors.affiliation: Department of Biological Sciences, Genetics and Molecular Biology Section, University of Naples Federico II, Naples, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Saccone
authors.forename: Marco
authors.initials: M
authors.lastname: Salvemini
authors.forename: Attilio
authors.initials: A
authors.lastname: Pane
authors.forename: Lino C
authors.initials: LC
authors.lastname: Polito
mesh_terms: D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D033622:Ceratitis capitata / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development*; D005260:Female; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D020033:Protein Isoforms / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation / Q000235:genetics; D013045:Species Specificity
chemical_list: D020413:3' Untranslated Regions; D017931:DNA Primers; D004268:DNA-Binding Proteins; C070369:DSX protein, Drosophila; D029721:Drosophila Proteins; D019476:Insect Proteins; D020033:Protein Isoforms