Effect of profound hypothermia on genomics of hippocampus following complete cerebral ischemia in rats.
OBJECTIVE
To determine differential gene expression of hippocampus in rats following complete cerebral ischemia with treatment of profound hypothermia compared to normothermia.


METHODS
Six rats got 5 minutes of complete cerebral ischemia with circulatory arrest and randomly divided into two groups: normothermia ischemia group (37 +/- 0.3 degrees C, n = 3) and profound hypothermia ischemia group (18 +/- 0.5 degrees C, n = 3). Affymetrix U34A rat arrays were applied to detect the difference of gene expression profile in hippocampus between the two groups.


RESULTS
Expression profiles of a total of 75 transcripts in the profound hypothermia ischemia group were statistically different from those of the normothermia ischemia group, and 33 of them were significantly up-regulated and other 42 were significantly down-regulated (p < 0.07).


CONCLUSIONS
Compared with normothermia, profound hypothermia had a significant effect on the gene expression profiles following complete cerebral ischemia, which may be involved in the mechanisms of cerebral protection by profound hypothermia.
Qin
Hua-Ping
HP
Department of Neurosurgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
Mei
Guang-Hai
GH
Wei
Liang
L
Jiang
Ji-Yao
JY
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology* / Q000628:therapy*; D030541:Databases, Genetic / Q000706:statistics & numerical data; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D023281:Genomics / Q000379:methods*; D006624:Hippocampus / Q000378:metabolism*; D007036:Hypothermia, Induced / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011897:Random Allocation; D051381:Rats