The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages.
Due to advances in stem cell biology, embryonic stem (ES) cells can be induced to differentiate into a particular mature cell lineage when cultured as embryoid bodies. Although transplantation of ES cells-derived neural progenitor cells has been demonstrated with some success for either spinal cord injury repair in small animal model, control of ES cell differentiation into complex, viable, higher ordered tissues is still challenging. Mouse ES cells have been induced to become neural progenitors by adding retinoic acid to embryoid body cultures for 4 days. In this study, we examine the use of electrospun biodegradable polymers as scaffolds not only for enhancing the differentiation of mouse ES cells into neural lineages but also for promoting and guiding the neurite outgrowth. A combination of electrospun fiber scaffolds and ES cells-derived neural progenitor cells could lead to the development of a better strategy for nerve injury repair.
Xie
Jingwei
J
Department of Biomedical Engineering, Washington University in St. Louis, Campus Box 1097, One Brookings Drive, St. Louis, MO 63130, USA.
Willerth
Stephanie M
SM
Li
Xiaoran
X
Macewan
Matthew R
MR
Rader
Allison
A
Sakiyama-Elbert
Shelly E
SE
Xia
Younan
Y
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D002470:Cell Survival; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000648:ultrastructure; D016501:Neurites / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D010641:Phenotype; D011091:Polyesters / Q000378:metabolism; D054457:Tissue Scaffolds
D011091:Polyesters; C016240:polycaprolactone