[Role of ventricular M3 receptor in arrhythmia resulted from cerebral-cardiac syndrome].
To detect the function and expression of ventricular M3 receptor (M3R) in cerebral-cardiac syndrome (CCS) model rats and to explore the relationship between the expression of M3R and the arrhythmia resulted from CCS, CCS model rats were induced by occluding right middle cerebral artery. ECG was monitored. Intracellular calcium ([Ca2+]i) changes after agitating M3R were recorded by laser scanning confocal microscope. Changes of M3R expression in the ventricular tissue were detected by Western blotting. QRS and QT intervals in CCS group were remarkably longer than that in sham group. According to the results of Western blotting, the level of M3R expression was remarkably lower in CCS group compared with that in the normal group. KCl induced [Ca2+]i increasing in CCS group could be depressed by choline and the effect of choline could be blocked by 4-DAMP. The lower expression of M3R in CCS group may be one of important reasons of arrhythmia resulted from CCS. M3R that depressed the [Ca2+]i increasing agitated by choline may become a new target to cure arrhythmia resulted from CCS.
Zhang
Gao-Xiao
GX
Bio-pharmaceutical Key laboratory of Heilongjiang Province-Incubator of State Key Laboratory, College of Pharmacy, Harbin Medical University, Harbin 150081, China.
Pan
Guo-Pin
GP
Sun
Li-Hua
LH
Zhang
Yan-Li
YL
Yang
Bao-Feng
BF
Wang
Ling
L
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D002118:Calcium / Q000378:metabolism*; D002794:Choline / Q000494:pharmacology; D004562:Electrocardiography; D006352:Heart Ventricles / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery / Q000150:complications*; D008297:Male; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D043587:Receptor, Muscarinic M3 / Q000037:antagonists & inhibitors / Q000378:metabolism*
D018727:Muscarinic Antagonists; D010880:Piperidines; D043587:Receptor, Muscarinic M3; D011189:Potassium Chloride; C042375:4-diphenylacetoxy-1,1-dimethylpiperidinium; D002794:Choline; D002118:Calcium