Expression of two different isoforms of fasciclin II during postembryonic central nervous system remodeling in Manduca sexta.
Insect metamorphosis serves as a useful model to investigate postembryonic development in the central nervous system, because the transformation between larval and adult life is accompanied by a remodeling of neural circuitry. Most changes are controlled by ecdysteroids, but activity-dependent mechanisms and cell surface signals also play a role. This immunocytochemical study investigates the expression patterns of two isoforms of the neural cell adhesion molecule, fasciclin II (FasII), during postembryonic ventral nerve cord remodeling in the moth, Manduca sexta. Both the expression of the glycosyl-phosphatidylinositol (GPI)-linked isoform and the transmembrane isoform of Manduca FasII (TM-MFasII) are regulated in a stereotyped spatio-temporal pattern. TM-MFasII is expressed in a stage-specific manner in a subset of neurons. Subsets of central axons express high levels during outgrowth supporting a functional role for TM-FasII during pathfinding. Dendritic localization is not found at any stage of metamorphosis, suggesting no homophilic interactions of TM-MFasII during central synapse development. GPI-MFasII is expressed in a stage-specific manner, most likely only in glial cells. The larval and adult stages show almost no GPI-MFasII expression, whereas during pupal life, positive GPI-MFasII labeling is present around synaptotagmin-negative tracts or commissures, so that either homophilic stabilization of glial boundaries or heterophilic neuron-glial interactions possibly stabilize the axons within their tracts. GPI-MFasII expression is not co-localized with synaptotagmin-positive central terminals, rendering a role for synapse development unlikely. Neither isoform is expressed in all neurons of a specific class at any developmental stage, indicating that MFasII functions are restricted to specific subsets of neurons or to individual neurons.
Kuehn
Claudia
C
School of Life Sciences, Arizona State University, Tempe, AZ 85287-4501, USA.
Duch
Carsten
C
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D002490:Central Nervous System / Q000254:growth & development* / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism; D005724:Ganglia / Q000166:cytology / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism; D007814:Larva / Q000166:cytology / Q000378:metabolism; D018402:Manduca / Q000254:growth & development* / Q000378:metabolism*; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism; D019581:Neuropil / Q000166:cytology / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D021381:Protein Transport; D011679:Pupa / Q000166:cytology / Q000378:metabolism
D015816:Cell Adhesion Molecules, Neuronal; D017261:Glycosylphosphatidylinositols; D020033:Protein Isoforms; C051642:fasciclin II