title: Synthesis and properties of a novel methoxy poly(ethylene glycol)-modified galactosylated chitosan derivative.
abstract: Chitosan and its derivatives are attractive non-viral vectors. To produce target-cell specificity and improve the solubility of chitosan, a novel chitosan derivative, modified with galactose and methoxy poly(ethylene glycol) (mPEG) was synthesized, and structure changes of chitosan and its derivatives were characterized. Compared to chitosan, the solution viscosity of the novel chitosan derivative drastically decreased. And, the degree of substitution (DS) of chitosan by galactose and mPEG were calculated as 0.09 and 0.30. The average diameter and zeta potential of mPEGylated galactosylated chitosan (GaC) nanoparticle containing VRMFat plasmid were 178 nm and +2.93 mV, suggesting suitable properties for gene delivery system. The gel electrophoresis confirmed that the plasmid DNA was remained completely by the mPEGylated GaC nanoparticle. And, the cytotoxic effect of mPEGylated GaC nanoparticles on human embryonic kidney (HEK 293) cells was negligible in comparison with that of control chitosans. Therefore, it is expected that the mPEGylated GaC will have the potential as a targeting gene delivery system for a further application.
authors.affiliation: College of Chemistry, Sichuan University, Chengdu, 610064, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Yuan Yuan
authors.initials: YY
authors.lastname: Yu
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Peng
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Jiang
authors.forename: Ping
authors.initials: P
authors.lastname: Dai
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D016503:Drug Delivery Systems; D005690:Galactose / Q000737:chemistry; D005822:Genetic Vectors; D006031:Glycosylation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010957:Plasmids / Q000235:genetics; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D048271:Chitosan; D005690:Galactose