title: A liquid chromatography/electrospray ionization mass spectrometry (LC-MS/MS) assay for the determination of irinotecan (CPT-11) and its two major metabolites in human liver microsomal incubations and human plasma samples.
abstract: A sensitive, rapid LC-MS/MS assay has been developed and validated for the simultaneous quantification of CPT-11 and its two principal metabolites, 7-ethyl-10-hydroxycamptothecin (SN-38), and 7-ethyl-10-[4-N-(5-aminopentanoic acid)-1-piperidino]carbonyloxy-camptothecin (APC) in human liver microsomal fractions and plasma. The method was linear over the ranges of 1.56-100 ng/mL, 3.13-150 ng/mL, and 0.78-100 ng/mL for CPT-11, SN-38, and APC, respectively. The total run time was 7.0 min. This assay offers advantages in terms of expediency, recovery of analytes, and suitability for the analysis of CPT-11 and its metabolites in various biological fluids.
authors.affiliation: Pharmacogenomics and Drug Development Group, Faculty of Pharmacy, The University of Sydney, NSW 2006, Australia. fabrizio@pharm.usyd.edu.au
authors.forename: Fabrizio
authors.initials: F
authors.lastname: D'Esposito
authors.forename: Bruce N
authors.initials: BN
authors.lastname: Tattam
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ramzan
authors.forename: Michael
authors.initials: M
authors.lastname: Murray
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000097:blood; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D000077146:Irinotecan; D008862:Microsomes, Liver / Q000737:chemistry*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C100720:RPR 121056; D000077146:Irinotecan; D002166:Camptothecin