Genomic sequence and biological characterization of a nucleopolyhedrovirus isolated from the summer fruit tortrix, Adoxophyes orana.
Adoxophyes orana nucleopolyhedrovirus (AdorNPV) was isolated from overwintering larvae from an orchard in the UK. The nucleotide sequence of the AdorNPV DNA genome was determined and analysed. The genome contains 111724 bp and has a G+C content of 35.0 mol%. The analysis predicted 121 ORFs of 150 nt or larger. Of these putative genes, 118 were homologous to genes identified previously in the Adoxophyes honmai nucleopolyhedrovirus (AdhoNPV) genome (83.3-100 % aa identity), and three AdorNPV ORFs were unique. There were four small homologous regions that consisted of a similar core sequence and at the same relative positions in the genome as AdhoNPV, but they differed in the number of repeats and orientation. Some genes that have been reported to have major roles in baculovirus biology were either absent or truncated in the AdorNPV genome. These included chitinase, which is involved in the liquefaction of the host, and the C-terminal of the ecdysteroid UDP-glucosyltransferase (egt) protein, which was truncated by 149 aa compared with AdhoNPV, with essential amino acids absent. The AdorNPV genome encoded two inhibitor of apoptosis (iap) genes compared with three in AdhoNPV and three bro genes compared with four in AdhoNPV. The susceptibility of A. orana larvae to AdorNPV was evaluated in laboratory bioassays using inoculation by microdroplet feeding and applied dose assays. LD50 for neonates was 56 occlusion bodies rising to 2.3x10(4) for fifth instar larvae. Median survival time values using an LD80 dose were 8.8 days for neonates and 7.0 days for fifth instar larvae.
Hilton
Sally
S
Warwick HRI, Wellesbourne, Warwick CV35 9EF, UK.
Winstanley
Doreen
D
Warwick HRI, Wellesbourne, Warwick CV35 9EF, UK.
D000595:Amino Acid Sequence; D000818:Animals; D016679:Genome, Viral; D007814:Larva / Q000821:virology*; D008969:Molecular Sequence Data; D009036:Moths / Q000254:growth & development / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid