title: Improving enzyme immobilization in mesocellular siliceous foams by microwave irradiation.
abstract: Microwave irradiation was used to immobilize papain and penicillin acylase in mesocellular siliceous foams (MCFs) at low temperature. The maximum loading of papain reached 984.1 mg/g, 1.26 times that obtained using the conventional, non-microwave-assisted method. The half-life (t(0.5)) of papain immobilized in MCFs by microwave irradiation at 80 degrees C was 17 h, 5.21 times that of papain immobilized by conventional means. The activities of papain and penicillin acylase immobilized with the microwave-assisted method were 779.6 U/mg and 141.8 U/mg respectively, 1.86 and 1.39 times of those obtained without microwave immobilization. Using microwave irradiation it only took 140 s for penicillin acylase, an enzyme of large dimensions, to be immobilized in MCFs. In contrast, it took 15 h to do the same using the conventional method. The results showed that microwave irradiation improved the adsorption and immobilization of enzymes in mesocellular siliceous foams.
authors.affiliation: National Engineering Research Center for Biotechnology, Nanjing 210009, P.R. China.
authors.forename: Anming
authors.initials: A
authors.lastname: Wang
authors.forename: Mingqing
authors.initials: M
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhou
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Du
authors.forename: Shemin
authors.initials: S
authors.lastname: Zhu
authors.forename: Shubao
authors.initials: S
authors.lastname: Shen
authors.forename: Pingkai
authors.initials: P
authors.lastname: Ouyang
mesh_terms: D000327:Adsorption / Q000528:radiation effects; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005740:Gases / Q000737:chemistry / Q000528:radiation effects; D008422:Materials Testing; D008872:Microwaves; D010206:Papain / Q000737:chemistry* / Q000528:radiation effects*; D010399:Penicillin Amidase / Q000737:chemistry* / Q000528:radiation effects*; D016062:Porosity / Q000528:radiation effects; D011485:Protein Binding / Q000528:radiation effects; D011829:Radiation Dosage; D012822:Silicon Dioxide / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D004800:Enzymes, Immobilized; D005740:Gases; D012822:Silicon Dioxide; D010206:Papain; D010399:Penicillin Amidase