title: Mutation in pfmdr1 gene in chloroquine-resistant Plasmodium falciparum isolates, Southeast Iran.
abstract: OBJECTIVE
  The main objective of the present study was to detect point mutations at positions 86, 184, 1034, 1042, and 1246 of the Plasmodium falciparum multidrug resistance gene (pfmdr1) in blood samples collected from malaria patients in Chabahar, a harbor city located in Southeast Iran.
  
  
  METHODS
  Twenty-six blood samples from patients infected with P. falciparum, who had a chloroquine (CQ) response failure, were collected pre-treatment. Following treatment with CQ, drug susceptibility was assessed using an in vivo test. Molecular detection of single nucleotide polymorphisms (SNPs) was carried out using the LightCycler hybridization probe assay.
  
  
  RESULTS
  The pfmdr1 N86Y mutation was found in six isolates (23.1%). Mutations at the four other positions were not observed in any isolates.
  
  
  CONCLUSIONS
  The present study showed no mutation at codon positions 184, 1034, 1042, and 1246 of pfmdr1 in any of the Iranian P. falciparum isolates; thus these alleles cannot serve as markers for CQ resistance in Iran.
authors.affiliation: Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, PO Box 14115-111, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Jalousian
authors.forename: Abdolhossein
authors.initials: A
authors.lastname: Dalimi
authors.forename: Siamak Mirab
authors.initials: SM
authors.lastname: Samiee
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Ghaffarifar
authors.forename: Faramarz
authors.initials: F
authors.lastname: Soleymanloo
authors.forename: Ramin
authors.initials: R
authors.lastname: Naghizadeh
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology* / Q000627:therapeutic use; D001483:Base Sequence; D002738:Chloroquine / Q000494:pharmacology* / Q000627:therapeutic use; D004351:Drug Resistance / Q000235:genetics*; D006801:Humans; D007492:Iran; D016778:Malaria, Falciparum / Q000188:drug therapy / Q000469:parasitology; D008969:Molecular Sequence Data; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000737:chemistry / Q000235:genetics*; D009154:Mutation; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D017211:Treatment Failure
chemical_list: D000962:Antimalarials; D002738:Chloroquine; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C513498:Mdr1 protein, Plasmodium falciparum