Experimental microencapsulation of porcine and rat pancreatic islet cells with air-driven droplet generator and alginate.
Transplantation of microencapsulated islets is proposed as an ideal therapy for the treatment of type 1 diabetes mellitus without immunosuppression. This strategy is based on the principle that foreign cells are protected from the host immune system by an artificial membrane. The aim of this study was to establish an ideal condition of microencapsulation using an air-driven droplet generator and alginate in vitro. The optimal conditions for islet encapsulation were an alginate inflow rate of 10 mL/h, CO2 flow rate of 2.0 L/min in a concentration of 2% alginate. For 2.5% alginate, the alginate inflow rate of 20 mL/h, CO2 flow rate 3.0 L/min was ideal; alginate inflow rate of 40 mL/h, CO2 flow rate of 4.0 L/min showed good microcapsules at 3% alginate. Viability of encapsulated islets was greater than 90%. In terms of insulin secretion, encapsulated islets secreted insulin in response to glucose in static culture medium. However, there was no normal response to low or high glucose challenge with a stimulation index less than 2.0. Microencapsulation of pig islets was successfully performed with air-driven droplet generator and alginate in vitro. Further studies about biocompatibility and glucose control in vivo may provide a useful tool for treatment of patients with diabetes mellitus.
Koo
S K
SK
Asan Institute for Life Science, Seoul, Korea.
Kim
S C
SC
Wee
Y M
YM
Kim
Y H
YH
Jung
E J
EJ
Choi
M Y
MY
Park
Y H
YH
Park
K T
KT
Lim
D G
DG
Han
D J
DJ
D000388:Air; D000464:Alginates; D000818:Animals; D002470:Cell Survival; D004339:Drug Compounding / Q000379:methods*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013552:Swine
D000464:Alginates; D007328:Insulin; D005947:Glucose