title: Two Amino Acid Substitutions in the Coat Protein of Pepper mild mottle virus Are Responsible for Overcoming the L(4) Gene-Mediated Resistance in Capsicum spp.
abstract: ABSTRACT The Capsicum spp. L genes (L(1) to L(4)) confer resistance to tobamoviruses. Currently, the L(4) gene from Capsicum chacoense is the most effective resistance gene and has been used widely in breeding programs in Japan which have developed new resistant cultivars against Pepper mild mottle virus (PMMoV). However, in 2004, mild mosaic symptoms began appearing on the leaves of commercial pepper plants in the field which possessed the L(4) resistance gene. Serological and biological assays on Capsicum spp. identified the causal virus strain as a previously unreported pathotype, P(1,2,3,4). PMMoV sequence analysis of the virus and site-directed mutagenesis using a PMMoV-J of the P(1,2) pathotype revealed that two amino acid substitutions in the coat protein, Gln to Arg at position 46 and Gly to Lys at position 85, were responsible for overcoming the L(4) resistance gene.
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Genda
authors.forename: Ayami
authors.initials: A
authors.lastname: Kanda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hamada
authors.forename: Kyoko
authors.initials: K
authors.lastname: Sato
authors.forename: Jun
authors.initials: J
authors.lastname: Ohnishi
authors.forename: Shinya
authors.initials: S
authors.lastname: Tsuda