title: Optimization of lipase-catalyzed transesterification of lard for biodiesel production using response surface methodology.
abstract: Biodiesel, an alternative diesel fuel made from renewable biological resources, has become more and more attractive recently. Combined use of two immobilized lipases with complementary position specificity instead of one lipase is a potential way to significantly reduce cost of lipase-catalyzed biodiesel production. In this study, the process of biodiesel production from lard catalyzed by the combined use of Novozym435 (non-specific) and Lipozyme TLIM (1,3-specific) was optimized by response surface methodology. The optimal reaction conditions were 0.04 of amount of lipase/oil (w/w), 0.49 of proportion of Novozym435/total lipases (w/w), 0.55 of quantity of tert-butanol/oil (v/v), 5.12 of quantity of methanol/oil (mol/mol), and 20 h of reaction time, by which 97.2% of methyl ester (ME) yield was attained, very close to the predicted value (97.6%). This optimal reaction condition could be true of other similar reactions with plant and animal oil resources; their ME yield could be higher than 95%. The lipases regenerated by washing with organic solvent after each reaction cycle could be continuously reused for 20 cycles without any loss of activity, exhibiting very high manipulation stability.
authors.affiliation: Huazhong University of Science & Technology, Wuhan, People's Republic of China. huangying@mail.hust.edu.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Hai
authors.initials: H
authors.lastname: Zheng
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Yan
mesh_terms: D056804:Biofuels / Q000032:analysis*; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D004041:Dietary Fats / Q000032:analysis*; D004800:Enzymes, Immobilized; D004951:Esterification; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D000432:Methanol / Q000032:analysis; D013499:Surface Properties
chemical_list: D056804:Biofuels; D004041:Dietary Fats; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; C093666:Lipozyme; C093667:Novozyme 435; D008049:Lipase; C029310:lard; D000432:Methanol