In vitro sonodynamic cytotoxicity in regulated cavitation conditions.
Sonodynamic toxicity has always been linked to the cavitation phenomenon. In this work, sonodynamic effect with Photofrin was evaluated with a new ultrasound device: a regulated cavitation generator. In this way, acoustic intensity was substituted with cavitation level as ultrasound parameter. Photofrin potentiated significantly the cavitation cytotoxicity even for low setpoints where no inertial cavitation appeared. Therefore sonodynamic mechanism was principally mechanical, facilitated by the Photofrin insertion in cellular cytoplasmic membranes. This assertion was also supported by the fact that sonodynamic cytotoxicity was independent from the Photofrin presence or absence in the extracellular medium. Reproducible sonodynamic efficiency was perfectly obtained with this new regulated cavitation generator.
El Maalouf
Jhony
J
INSERM, U556, Université de Lyon, Lyon F-69003, France. el-maalouf@lyon.inserm.fr
Béra
Jean-Christophe
JC
Alberti
Laurent
L
Cathignol
Dominique
D
Mestas
Jean-Louis
JL
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival; D003603:Cytotoxins / Q000494:pharmacology*; D017323:Dihematoporphyrin Ether / Q000494:pharmacology*; D004867:Equipment Design; D066298:In Vitro Techniques; D051381:Rats; D012044:Regression Analysis; D014407:Tumor Cells, Cultured; D014465:Ultrasonics
D000970:Antineoplastic Agents; D003603:Cytotoxins; D017323:Dihematoporphyrin Ether