[Evidence on the role of HLA and MICA antibodies in renal graft loss].
BACKGROUND
HLA and MICA antibodies are increasingly associated with poorer graft survival. The aim of this study is to report the frequency of graft loss 2 years after the detection of HLA abs and MICA abs among a group of kidney transplant recipients.


METHODS
We tested 196 patients with a functioning graft. Sera were screened for HLA and MICA IgG abs by Luminex, using the LABScreen Mixed, and LABScreen PRA. The sera were screened for MICA abs by Luminex.


RESULTS
Of 196 kidney transplant recipients (mean age 36.7 years, 42% female), one hundred twenty four (63.3%) were negative to all tested abs, and 72 (36.7%) were positive for: HLA abs alone = 34, MICA abs alone = 29, and HLA+MICA abs = 9. At a median followup of 20.5 (1.2-25.2) months, 8 patients lost their grafts due to biopsy-confirmed chronic allograft injury: 2/124 (1.6%) ab-negative, and 6/72 (8.3%) ab-positive, with a significantly lower survival for the Ab-positive group (p = 0.046, log-rank test).


CONCLUSIONS
The presence of circulating abs was associated with an increased risk of graft loss, and the coexistence of HLA and MICA abs increases the risk of graft loss.
Morales-Buenrostro
Luis E
LE
Departamentos de Nefrología-Metabolismo Mineral, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán", México DF, México.
Rodríguez-Romo
Roxana
R
de Leo-Cervantes
Claudia
C
López
Mayra
M
Pérez-Garrido
Jesús
J
Uribe-Uribe
Norma
N
Alberú-Gómez
Josefina
J
D000328:Adult; D005260:Female; D006084:Graft Rejection / Q000097:blood* / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D016030:Kidney Transplantation; D008297:Male
D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D007074:Immunoglobulin G; C099187:MHC class I-related chain A