A small amount of tetrachloroethylene ingestion from drinking water accelerates antigen-stimulated allergic responses.
Previously, we observed that tetrachloroethylene (perchloroethylene, PCE) increased histamine release and inflammatory mediator production from antigen-stimulated mast cells. In this study, we examined the enhancing effect of low concentrations of PCE in drinking water on antigen-stimulated allergic responses. After exposure of Wistar rats to PCE in drinking water for 2 or 4 weeks, we performed a passive cutaneous anaphylaxis (PCA) reaction. PCE exposure for 4 weeks enhanced PCA reaction in a dose-dependent manner. In pathological studies, PCE exposure for 2 weeks exacerbated inflammation characterized by infiltration of lymphocytes and accumulation of mast cells around the vessel. Non-purified mast cells (NPMCs) from rats treated with 1mg/L PCE in drinking water for 2 weeks increased antigen-stimulated histamine release. Furthermore, the leukocytes of rats treated with PCE in drinking water for 4 weeks showed increased interleukin (IL)-4 expression. The mechanism of enhancing the PCA reaction is assumed to be that PCE increases IL-4 production and PCE causes T helper (Th) 1/Th2-type helper T-cell imbalance and increases histamine release from excessively accumulated mast cells. The results suggest that the intake of PCE in drinking water, even at a low concentration, leads to the initiation and acceleration of allergic diseases.
Seo
Makoto
M
Laboratory of Hygienics, Gifu Pharmaceutical University, Gifu, Japan. mkt.s.lev5060@gmail.com
Yamagiwa
Takeo
T
Kobayashi
Ryo
R
Ikeda
Koji
K
Satoh
Masahiko
M
Inagaki
Naoki
N
Nagai
Hiroichi
H
Nagase
Hisamitsu
H
D000818:Animals; D000941:Antigens / Q000276:immunology; D015550:Cell Degranulation / Q000187:drug effects*; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004140:Dinitrophenols / Q000276:immunology; D004326:Drinking; D006636:Histamine Release / Q000187:drug effects*; D006967:Hypersensitivity / Q000276:immunology*; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013750:Tetrachloroethylene / Q000008:administration & dosage* / Q000276:immunology; D014867:Water
D000941:Antigens; D016207:Cytokines; D004140:Dinitrophenols; D014867:Water; D013750:Tetrachloroethylene