[Cytokine contents in single donor platelets during storage].
This study was aimed to investigate the changes of cytokine contents in single donor platelets (SDPs) collected by using MCS(+), Trima, Amicus blood cell separators during storage. 18 portions of SDPs were collected by MCS(+), Trima, Amicus blood cell separators, were preserved in standard condition of blood bank, the levels of cytokines such as IL-8, RANTES, CD154, TGF-beta(1) and VEGF were detected by ELISA at 1, 3, 5, 7 days during storage. The results showed that the levels of IL-8, RANTES, CD154, TGF-beta(1) and VEGF in SDPs collected by blood cell separators MCS(+), Trima, and Amicus gradually increased with prolonging of time during storage, but the increase of IL-8 level in SDPs collected by MCS(+) separator was significant difference from SDPs collected by Trime and Amicus separators (p < 0.05). It is concluded that the all collected SDPs mentioned above express IL-8, RANTES, CD154, TGF-beta(1) and VEGF during storage, and their cytokine levels show a tendency to increase with prolonging of time during storage, apheresis platelets with less leukocytes express IL-8 lower.
Xu
Jian
J
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Shen
Zhuo-Lan
ZL
Yu
Li
L
Yang
Jin
J
Yu
Ron
R
Men
Zhong-Hua
ZH
Lu
Hang-Jun
HJ
Yan
Li-Xing
LX
D001792:Blood Platelets / Q000378:metabolism*; D023201:CD40 Ligand / Q000097:blood; D002469:Cell Separation / Q000379:methods; D018946:Chemokine CCL5 / Q000097:blood; D016207:Cytokines / Q000097:blood*; D006801:Humans; D016209:Interleukin-8 / Q000097:blood; D010976:Platelet Count; D013048:Specimen Handling; D016212:Transforming Growth Factor beta / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
C483024:CCL5 protein, human; C508606:CXCL8 protein, human; D018946:Chemokine CCL5; D016207:Cytokines; D016209:Interleukin-8; D016212:Transforming Growth Factor beta; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D023201:CD40 Ligand