Application of liquid chromatography-electrospray ionization time-of-flight mass spectrometry for analysis and quality control of compound Danshen preparations.
A liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry (LC-ESI-TOF-MS) method has been developed to evaluate the quality of three formulas of compound Danshen preparations (CDPs), through a simultaneous determination of 22 marker constituents (nine major phenolic acids, eight major saponins and five major diterpenoids). Optimum separations were obtained with a Zorbax C(18) column, using a gradient elution with 0.1% aqueous formic acid and acetonitrile. Limits of detection and quantification were in ranges of 1.58-10.10 and 4.85-28.56 ng/mL. All calibration curves showed good linear regression (r(2 ) > 0.9900) within the test range, and the recoveries were between 78.4 and 103.1% for all analytes. The assay was successfully utilized to analyze the 22 marker components in 26 samples. The overall results demonstrated that this method is sensitive, accurate and reliable for the quality control of CDPs.
Cao
Jun
J
Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education, Nanjing 210009, People's Republic of China.
Qi
Lian-Wen
LW
Chen
Jun
J
Yi
Ling
L
Li
Ping
P
Ren
Mei-Ting
MT
Li
Yan-Jing
YJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D028321:Plant Preparations / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D028321:Plant Preparations