title: Danshen protects kidney grafts from ischemia/reperfusion injury after experimental transplantation.
abstract: Danshen (DS) is used for treatment of various ischemic events in the traditional Chinese medicine. Hence, this study was designed to investigate its effect on ischemia/reperfusion injury (IRI) after experimental kidney transplantation (eKTx). Nephrectomized Sprague-Dawley rats underwent eKTx. Some animals were infused with 1.5 ml DS 10 min before surgery. Kidney grafts were transplanted after cold storage for 20 h in Histidine-Tryptophane-Ketoglutarate solution. After reperfusion blood samples were collected for blood urinary nitrogen (BUN), creatinine, lactate dehydrogenase (LDH), and alanine transaminase. Further, tissue was assessed for morphologic and pathophysiologic changes. Donor preconditioning with DS (DS-d) significantly decreased BUN, creatinine, LDH, and aspartate aminotransferase to 65-97% of controls while preconditioning of the recipient (DS-r) decreased values to 58-82% (P < 0.05). Tubular damage and caspase-3 decreased significantly in both DS-d and DS-r (DS-d: 96% and 67%, DS-r: 83% and 75% of controls) while heat shock protein 72 and superoxide dismutase increased significantly (DS-d: 143% and 173%, DS-r: 166% and 194% of controls). Further, inducible nitric oxide synthase and tumor necrosis factor-alpha decreased (DS-d: 84% and 61%, DS-r: 79% and 67% of controls) after DS. Preconditioning of both donors and recipients with DS significantly reduces IRI and thus improves graft function after eKTx.
authors.affiliation: Department of General Surgery, Ruprecht-Karls-University, Heidelberg, Germany.
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Guan
authors.forename: Genevieve
authors.initials: G
authors.lastname: Dei-Anane
authors.forename: Helge
authors.initials: H
authors.lastname: Bruns
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Arash
authors.initials: A
authors.lastname: Nickkholgh
authors.forename: Rui
authors.initials: R
authors.lastname: Liang
authors.forename: Marie-Luise
authors.initials: ML
authors.lastname: Gross
authors.forename: Michael
authors.initials: M
authors.lastname: Kern
authors.forename: Jochen
authors.initials: J
authors.lastname: Ludwig
authors.forename: Markus W
authors.initials: MW
authors.lastname: Büchler
authors.forename: Peter
authors.initials: P
authors.lastname: Schemmer
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007674:Kidney Diseases / Q000209:etiology / Q000517:prevention & control*; D016030:Kidney Transplantation / Q000009:adverse effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D027561:Salvia miltiorrhiza
chemical_list: D010936:Plant Extracts