(-)-Catechin promotes adipocyte differentiation in human bone marrow mesenchymal stem cells through PPAR gamma transactivation.
Green tea intake has been shown to confer various health benefits to patients suffering from metabolic disorders. Here, we studied the effect of several major green tea polyphenols on adipocyte differentiation in human bone marrow mesenchymal stem cells (hBM-MSCs) and compared it to the effect of representative antidiabetic drugs. (-)-Catechin was the most potent of the eight green tea polyphenols evaluated in promoting adipocyte differentiation in hBM-MSCs, and this effect was dose-dependent. (-)-Catechin increased the mRNA levels of various adipogenic markers, such as adiponectin, peroxisome proliferator-activated receptor gamma (PPARgamma), FABP4, and LPL, as measured during adipocyte differentiation in hBM-MSCs. In addition, (-)-catechin upregulated the secretion of adiponectin in hBM-MSC culture. Using a reporter gene assay and a competitive ligand binding study, (-)-catechin also significantly activated PPARgamma in a dose-dependent fashion; however, (+)-catechin, the enantiomer of (-)-catechin, was not effective as a PPARgamma agonist, which seems to imply that the effect of (-)-catechin on PPARgamma is stereospecific. In conclusion, our data suggest that (-)-catechin promotes adipocyte differentiation and increased sensitivity to insulin in part by direct activation of PPARgamma, which could be at the basis of the observed pharmacological benefits of green tea intake in reducing the risk of type 2 diabetes.
Shin
Dong Wook
DW
Biosystems Research, AmorePacific Corporation R&D Center, 314-1, Bora-dong, Kiheung-gu Yongin, Gyeounggi-do 446-729, Republic of Korea.
Kim
Su Nam
SN
Lee
Sang Min
SM
Lee
Woojung
W
Song
Min Jeong
MJ
Park
Sun Mi
SM
Lee
Tae Ryong
TR
Baik
Joo-Hyun
JH
Kim
Han Kon
HK
Hong
Jeong-Ho
JH
Noh
Minsoo
M
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002392:Catechin / Q000494:pharmacology / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects / Q000502:physiology
D047495:PPAR gamma; D002392:Catechin