Protocols for nuclei isolation and nuclear protein extraction from the resurrection plant Xerophyta viscosa for proteomic studies.
The plant nucleus is an important subcellular organelle but the isolation of pure and enriched nuclei from plants and subsequent extraction of nuclear proteins for proteomic studies is challenging. Here, we present protocols for nuclei isolation and nuclear protein extraction from the resurrection plant, Xerophyta viscosa, and show optimization and modification of the most critical steps.
Abdalla
Kamal Omer
KO
Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch 7701, South Africa.
Thomson
Jennifer Ann
JA
Rafudeen
Muhammad Suhail
MS
D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus / Q000737:chemistry* / Q000378:metabolism; D032181:Craterostigma / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D009687:Nuclear Proteins / Q000302:isolation & purification*; D010940:Plant Proteins / Q000302:isolation & purification*; D040901:Proteomics
D009687:Nuclear Proteins; D010940:Plant Proteins