[The application of combined detection of autoantibodies in primary Sjögren's syndrome].
OBJECTIVE
To compare the specificity and sensitivity of antibodies against SSA and SSB, anti-M3 receptor polypeptide antibodies, anti-alpha-fodrin (IgG) antibodies in primary Sjögren's syndrome (pSS).


METHODS
One hundred and ten pSS patients (mean age was 49.2 +/- 14. 8, mean disease duration was 5.6 +/- 4.6), 80 systemic lupus erythematosus (SLE) patients (mean age was 25.5 +/- 4.6, mean disease duration was 2.5 +/- 1.2) and 80 rheumatoid arthritis (RA) patients (mean age was 44.6 +/- 3.5, mean disease duration was 4.2 +/- 1.1) were studied. Enzyme-linked immunosorbent assay was used to measure these antibodies.


RESULTS
The seropositive rates of anti-SSA, anti-SSB antibodies, anti-M3 receptor polypeptide antibodies and anti-alpha-fodrin (IgG) antibodies were 45.5%, 30.9%,78.2% and 77.3%, respectively in pSS. They were much higher than those in RA and SLE patients (P < 0.05). Specificities of SSA, SSB antibodies, anti-M3 receptor antibodies and anti-alpha-fodrin IgG were 83.8%, 97.7%, 92.0% and 90.0% respectively. With the combination of these antibodies in the diagnosis of pSS, the sensitivity can be increased at least to 88.2% and the specificity was not decreased significantly.


CONCLUSIONS
Combination of these antibodies can significantly improve the sensitivity of these antibodies in the diagnosis of pSS. Anti-SSA and SSB antibodies, anti-M3 receptor antibodies and anti-alpha-fodrin (IgG) antibodies are specific antibodies for the diagnosis of pSS.
He
Jing
J
Department of Rheumatology & Immunology, People' s Hospital, Peking University, Beijing 100044, China.
Fang
Wan
W
Li
Zhan-Guo
ZG
D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000097:blood; D000974:Antibodies, Antinuclear / Q000097:blood; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D002352:Carrier Proteins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008840:Microfilament Proteins / Q000276:immunology; D008875:Middle Aged; D043587:Receptor, Muscarinic M3 / Q000276:immunology; D012680:Sensitivity and Specificity; D012859:Sjogren's Syndrome / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D055815:Young Adult; D013049:Spectrin
D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D002352:Carrier Proteins; D008840:Microfilament Proteins; D043587:Receptor, Muscarinic M3; D013049:Spectrin; C035356:SS-A antibodies; C035357:SS-B antibodies; C048844:anti-IgG