title: [Differentially expressed genes involving the correlativity between heart meridian and hypothalamus].
abstract: OBJECTIVE
  To study the bio-molecule basis of correlativity between Heart Meridian and hypothalamus from gene differential expression.
  
  
  METHODS
  Twenty-eight healthy Sprague-Dawley male rats were divided into 4 groups randomly: normal control, model, Heart Meridian-electroacupuncture (EA) and Lung Meridian-EA groups, with 7 cases in each. Acute myocardial ischemia (AMI) model was duplicated by occlusion of the left anterior descending branch (LAD) of the coronary artery under anesthesia (3% Nemb, 40 mg/kg). EA (1.1 mA, 2 Hz, duration of waves 300 micros) was applied to bilateral "Shenmen" (HT 7)-"Tongli" (HT 5, Heart Meridian), "Taiyuan" (LU 9)-"Lieque" (LU 7, Lung Meridian) separately for 20 min, once daily for 3 days. Then, hypothalamus tissue was taken for differentially expressed gene profiling analysis (Affymetrix GeneChip Rat Genome U 230 Affymetrix).
  
  
  RESULTS
  Compared with model group, among the differentially expressed genes above 2 times in Heart-Meridian-EA group and Lung-Meridian-EA group, 190 and 57 were up-regulated, 34 and 26 down-regulated separately. Compared with Lung-Meridian-EA group, among the 175 differentially expressed genes above 2 times in hypothalamus tissues of Heart-Meridian-EA group, 147 were up-regulated and 28 down-regulated.
  
  
  CONCLUSIONS
  In comparison with EA of Lung Meridian, a different gene expression profile of hypothalamus tissue is involved in the effect of EA of Heart-Meridian on acute myocardial ischemia.
authors.affiliation: Center for Researching Meridian-Viscera Correlation, Anhui College of Chinese Medicine, Hefei 230038, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Di-he
authors.initials: DH
authors.lastname: Long
authors.forename: Lu
authors.initials: L
authors.lastname: He
authors.forename: Ling
authors.initials: L
authors.lastname: Hu
authors.forename: Ke-ming
authors.initials: KM
authors.lastname: Wang
authors.forename: Zi-jian
authors.initials: ZJ
authors.lastname: Wu
authors.forename: Rong-lin
authors.initials: RL
authors.lastname: Cai
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Zhou
mesh_terms: D000818:Animals; D015671:Electroacupuncture; D015870:Gene Expression; D020869:Gene Expression Profiling; D006321:Heart / Q000503:physiopathology*; D006801:Humans; D007031:Hypothalamus / Q000503:physiopathology*; D008297:Male; D016740:Meridians; D017202:Myocardial Ischemia / Q000503:physiopathology / Q000628:therapy*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley