title: Characterisation of glycoforms of ascitic fluids in benign and malignant diseases.
abstract: OBJECTIVE
  To evaluate the differences in glycoforms of ascitic fluids which can be considered as useful in discrimination between benign and malignant patients.
  
  
  METHODS
  The investigations were carried out on 17 benign and 13 malignant patients. To obtain high molecular weight material, gel exclusion chromatography was applied. To evaluate the relative amounts of different glycoforms, ELISA tests with biotinylated lectins were used.
  
  
  RESULTS
  The fucosylation pattern was found to be similar in malignant and benign group. Fucose linked by alpha 1-6 bond was the most abundant structure. Sialylation was found to be more typical for malignant patients. Alpha 2-6 bond was observed on higher level than alpha 2-3 linkage. T and Tn antigens were present on rather low level with a slight prevalence of T antigen in malignant group. The glycoforms recognized by DSA lectin were more numerous in benign patients.
  
  
  CONCLUSIONS
  The evaluation of the level of some ascitic fluids glycoforms can be useful in differentiation between malignant and benign diseases.
authors.affiliation: Department of Medical Chemistry, Medical University of Białystok, ul. Mickiewicza 2a, 15-222 Białystok 8, Poland. iwona@umwb.edu.pl
authors.forename: Iwona
authors.initials: I
authors.lastname: Radziejewska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Borzym-Kluczyk
authors.forename: Dariusz G
authors.initials: DG
authors.lastname: Kisiel
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Namiot
authors.forename: Joanna
authors.initials: J
authors.lastname: Wosek
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gindzieński
mesh_terms: D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry; D000957:Antigens, Viral, Tumor / Q000737:chemistry; D001202:Ascitic Fluid / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005643:Fucose / Q000737:chemistry*; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000737:chemistry; D008297:Male; D018396:Mucin-1 / Q000737:chemistry; D009369:Neoplasms / Q000737:chemistry*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D000957:Antigens, Viral, Tumor; D037102:Lectins; D018396:Mucin-1; C040286:Tn antigen; D005643:Fucose