Inhibition of host protein synthesis in B95a cells infected with the HL strain of measles virus.
The shut-off of host protein synthesis in virus-infected cells is one of the important mechanisms for viral replication. In this report, we showed that the HL strain of measles virus (MeV-HL) as well as other field isolates, which were isolated from human blood lymphocytes using B95a cells, induce the shut-off in B95a cells. Since the Edmonston strain of MeV failed to induce the shut-off in B95a cells, the ability to induce the shut-off was considered to be dependent on virus strains. Although, the modification of eukaryotic translation initiation factors (eIF) including eIF4G, eIF4E, and 4E-BP1 was reported for shut-off by various viruses, the involvement of these eIFs was not observed in MeV-HL-infected B95a cells. Instead, the accumulation of phosphorylated eIF2alpha was found to coincide to the decrease of host protein synthesis, suggesting the involvement of phosphorylation of eIF2alpha in inhibition of translation as one of the mechanisms of the shut-off.
Inoue
Yoshihisa
Y
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Tsukiyama-Kohara
Kyoko
K
Yoneda
Misako
M
Sato
Hiroki
H
Kai
Chieko
C
D000818:Animals; D002460:Cell Line; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D039561:Eukaryotic Initiation Factor-4E / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D039603:Eukaryotic Initiation Factor-4G / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D054884:Host-Pathogen Interactions; D006801:Humans; D008457:Measles / Q000378:metabolism*; D008459:Measles virus / Q000502:physiology*; D010766:Phosphorylation; D014176:Protein Biosynthesis; D039381:RNA Cap-Binding Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
D015852:Eukaryotic Initiation Factor-2; D039561:Eukaryotic Initiation Factor-4E; D039603:Eukaryotic Initiation Factor-4G; D039381:RNA Cap-Binding Proteins