Determination of glyphosate, glyphosate metabolites, and glufosinate in human serum by gas chromatography-mass spectrometry.
This paper describes an assay for the determination of glyphosate (GLYP), glyphosate metabolites [(aminomethyl) phosphonic acid] (AMPA), and glufosinate (GLUF) in human serum. After protein precipitation using acetonitrile and solid-phase extraction, serum samples were derivatized and analyzed by gas chromatography-mass spectrometry (GC-MS). The assay was linear over a concentration range of 3-100.0 microg/ml for GLYP, AMPA, and GLUF. The overall recoveries for the three compounds were >73%. The intra- and inter-day variations were <15%. Precision and accuracy were 6.4-10.6% and 88.2-103.7%, respectively. The validated method was applied to quantify the GLYP and AMPA content in the serum of a GLYP-poisoned patient. In conclusion, the method was successfully applied for the determination of GLYP and its metabolite AMPA in serum obtained from patient of GLYP-poisoning.
Motojyuku
Megumi
M
Department of Emergency and Critical Care Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1143, Japan.
Saito
Takeshi
T
Akieda
Kazuki
K
Otsuka
Hiroyuki
H
Yamamoto
Isotoshi
I
Inokuchi
Sadaki
S
D000613:Aminobutyrates / Q000097:blood* / Q000506:poisoning / Q000652:urine; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives* / Q000097:blood / Q000506:poisoning / Q000652:urine; D006540:Herbicides / Q000097:blood* / Q000506:poisoning / Q000652:urine; D006801:Humans; D007555:Isoxazoles; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D063065:Organophosphonates / Q000097:blood* / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013406:Suicide, Attempted; D013777:Tetrazoles; D000097797:Glyphosate
D000613:Aminobutyrates; D005998:Glycine; D006540:Herbicides; D007555:Isoxazoles; D063065:Organophosphonates; D013777:Tetrazoles; D000097797:Glyphosate; C003121:phosphinothricin; C418233:2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid