title: Cytokine profile associated with chronic and acute human schistosomiasis mansoni.
abstract: The production and regulation of interleukin (IL) IL-13, IL-4 and interferon-gamma (IFN-gamma) was evaluated in 43 schistosomiasis patients with different clinical forms. Whole-blood cultures cytokine production in response to soluble egg antigen (SEA), soluble worm adult preparation (SWAP), mitogens, neutralizing antibodies or recombinant IL-13 were measured by ELISA. After SWAP stimulation, chronic patients, particularly hepatointestinals, produced higher levels of IL-4 in comparison with acute patients, suggesting the presence of a type 2 cytokine profile in these patients. Following SEA and SWAP stimulation, hepatosplenic (HS) patients showed increased levels of IFN-gamma when compared with acute patients, indicating that HS disease in humans is associated with a type 1 cytokine response. The mechanisms of immune regulation are apparently different between the clinical stages of the disease, some of which are antigen-specific.
authors.affiliation: Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE, Brasil. clarice@cpqam.fiocruz.br
authors.forename: Clarice Neuenschwander Lins de
authors.initials: CN
authors.lastname: Morais
authors.forename: Joelma Rodrigues de
authors.initials: JR
authors.lastname: Souza
authors.forename: Wlademir Gomes
authors.initials: WG
authors.lastname: Melo
authors.forename: Marcílio L
authors.initials: ML
authors.lastname: Aroucha
authors.forename: Paulo
authors.initials: P
authors.lastname: Miranda
authors.forename: Ana Lúcia Coutinho
authors.initials: AL
authors.lastname: Domingues
authors.forename: Frederico Guilherme Coutinho
authors.initials: FG
authors.lastname: Abath
authors.forename: Silvia Maria Lucena
authors.initials: SM
authors.lastname: Montenegro
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007377:Interleukin-3 / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D007377:Interleukin-3; D015847:Interleukin-4; D007371:Interferon-gamma