[Change in T cell-mediated immunity and its relationship with high mobility group box-1 protein levels in extensively burned patients].
OBJECTIVE
To investigate the change in T cell-mediated immunity and its relationship with plasma high mobility group box-1 protein (HMGB1) levels in severely burned patients.


METHODS
Thirty-five extensively burned patients (> 30% total body surface area) were included in this study, and were divided into MODS group (n = 13) and non-MODS group (n = 22). The blood samples were collected on post burn days 1, 3, 5, 7, 14, 21 and 28. The plasma levels of HMGB1 were measured by using ELISA, and T lymphocyte proliferation response and its IL-2 production ability in peripheral blood were determined too. In addition, the ratio of CD4+/CD8+ T cells were detected by using flow cytometry.


RESULTS
Plasma HMGB1 levels were markedly elevated on post burn day 1 in severely burned patients, and HMGB1 level was significantly higher in MODS group than in non-MODS group (P < 0.05). Lymph proliferation response and IL-2 production of T cells in peripheral blood, and the ratio of CD4+/CD8+ T cells in MODS group were markedly lower than those in non-MODS group on post burn days 1, 14, 21 and 28 (all P < 0.05). It indicated that plasma HMGB1 levels were negatively correlated to T cellular immune function parameters, including lymphocyte proliferation response, IL-2 production, and the ratio of CD4+/ CD8+ T cells in extensively burned patients (all P < 0.05).


CONCLUSIONS
Extensive burns could lead to T cellular immune dysfunction, which appears to be associated with the development of MODS. HMGB1, as an important late mediators of inflammation, might be involved in the pathogenesis of suppression of T cell-mediated immunity in these patients.
Dong
Ning
N
Burns Institute, First Hospital Affiliated to the Chinese Public Liberate Army General Hospital, Beijing 100037, China.
Jin
Bo-Quan
BQ
Yao
Yong-Ming
YM
Yu
Yan
Y
Cao
Yu-Jue
YJ
He
Li-Xin
LX
Chai
Jia-Ke
JK
Sheng
Zhi-Yong
ZY
D000293:Adolescent; D000328:Adult; D002056:Burns / Q000097:blood / Q000150:complications / Q000276:immunology*; D005260:Female; D024243:HMGB1 Protein / Q000097:blood*; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000209:etiology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D024243:HMGB1 Protein