title: The basic helix-loop-helix transcription factor olig2 is critical for reactive astrocyte proliferation after cortical injury.
abstract: The mechanisms underlying the formation of the glial scar after injury are poorly understood. In this report, we demonstrate that after cortical injury Olig2 is upregulated in reactive astrocytes coincident with proliferation of these cells. Short-term lineage tracing studies with glial subtype-restricted transgenic reporter lines indicate that Olig2-expressing cells in the astroglial but not the oligodendroglial lineage are the essential source of reactive astrocytes. In addition, cortical Olig2 ablation results in a decrease in proliferation of reactive astrocytes in response to injury. Cell-type-specific mutagenesis indicates that Olig2 ablation in GFAP+ astrocytes and their precursors rather than in neuronal or oligodendroglial cells is responsible for the reduction of reactive astrocyte proliferation. Thus, our studies suggest that Olig2 is critical for postinjury gliosis.
authors.affiliation: Department of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Darryl K
authors.initials: DK
authors.lastname: Miles
authors.forename: Thaonguyen
authors.initials: T
authors.lastname: Hoang
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.forename: Edward
authors.initials: E
authors.lastname: Hurlock
authors.forename: Steven G
authors.initials: SG
authors.lastname: Kernie
authors.forename: Q Richard
authors.initials: QR
authors.lastname: Lu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000502:physiology*; D001930:Brain Injuries / Q000473:pathology*; D019070:Cell Lineage; D049109:Cell Proliferation; D002540:Cerebral Cortex / Q000473:pathology*; D005786:Gene Expression Regulation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D004676:Myelin Basic Protein / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D064231:Nestin; D000076104:Oligodendrocyte Transcription Factor 2; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D064231:Nestin; D000076104:Oligodendrocyte Transcription Factor 2; D020796:Receptor, Platelet-Derived Growth Factor alpha; C577485:Nes protein, mouse; C512773:Olig2 protein, mouse