title: [Screen and identification of genes involved in multidrug resistance in Pseudomonas aeruginosa].
abstract: OBJECTIVE
  To identify genes involved in multidrug resistance in Pseudomonas aeruginosa.
  
  
  METHODS
  We constructed and screened a transposon mutation library and obtained mutants that exhibited decreased drug resistance. The insertion sites of the transposon were identified by arbitrary PCR and subsequent DNA sequencing.
  
  
  RESULTS
  Two mutants which became more susceptible to several antibacterial agents than the wild type were identified as mutants with transposon insertion in gene PA2580 and PA2800 respectively. The functions of these two genes are described as unknown in P. aeruginosa database.
  
  
  CONCLUSIONS
  PA2580 and PA2800 are involved in drug resistance, possibly through their roles in redox reactions and cell envelope biogenesis.
authors.affiliation: Molecular Microbiology Laboratory, Northwest University, Xi,an 710069, China. zxyyt0801@163.com
authors.forename: Xingyan
authors.initials: X
authors.lastname: Zhao
authors.forename: Haihua
authors.initials: H
authors.lastname: Liang
authors.forename: Lixin
authors.initials: L
authors.lastname: Shen
authors.forename: Zhaolin
authors.initials: Z
authors.lastname: Dong
authors.forename: Kangmin
authors.initials: K
authors.lastname: Duan
mesh_terms: D000595:Amino Acid Sequence; D004251:DNA Transposable Elements / Q000235:genetics; D030541:Databases, Genetic; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements