title: The Drosophila STIM1 orthologue, dSTIM, has roles in cell fate specification and tissue patterning.
abstract: BACKGROUND
  Mammalian STIM1 and STIM2 and the single Drosophila homologue dSTIM have been identified as key regulators of store-operated Ca2+ entry in cells. STIM proteins function both as molecular sensors of Ca2+concentration in the endoplasmic reticulum (ER) and the molecular triggers that activate SOC channels in the plasma membrane. Ca2+ is a crucial intracellular messenger utilised in many cellular processes, and regulators of Ca2+ homeostasis in the ER and cytosol are likely to play important roles in developmental processes. STIM protein expression is altered in several tumour types but the role of these proteins in developmental signalling pathways has not been thoroughly examined.
  
  
  RESULTS
  We have investigated the expression and developmental function of dSTIM in Drosophila and shown that dSTIM is widely expressed in embryonic and larval tissues. Using the UAS-Gal4 induction system, we have expressed full-length dSTIM protein and a dsRNAi construct in different tissues. We demonstrate an essential role for dSTIM in larval development and survival, and a tissue-specific role in specification of mechanosensory bristles in the notum and specification of wing vein thickness.
  
  
  CONCLUSIONS
  Our studies show that dSTIM regulates growth and patterning of imaginal discs and indicate potential interactions with the Notch and Wingless signaling pathways. These interactions may be relevant to studies implicating STIM family proteins in tumorigenesis.
authors.affiliation: Department of Anatomy and Cell Biology, University of Melbourne, Victoria 3010, Australia. jpeid1@googlemail.com
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Eid
authors.forename: Alfonso Martinez
authors.initials: AM
authors.lastname: Arias
authors.forename: Hannah
authors.initials: H
authors.lastname: Robertson
authors.forename: Gary R
authors.initials: GR
authors.lastname: Hime
authors.forename: Marie
authors.initials: M
authors.lastname: Dziadek
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D004330:Drosophila / Q000196:embryology* / Q000235:genetics / Q000254:growth & development*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D000071737:Stromal Interaction Molecule 1; D051155:Wnt1 Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; C502662:Stim protein, Drosophila; D000071737:Stromal Interaction Molecule 1; D051155:Wnt1 Protein; C074622:wg protein, Drosophila; D002118:Calcium