title: Performance enhancement of polyaniline-based polymeric wire biosensor.
abstract: We demonstrate here the performance enhancement of polyaniline-based biosensor using screen-printing technology and pulse mode measurement technique. Screen-printed silver electrodes were made on a nitrocellulose membrane and the distance between the two electrodes was approximately 550 microm. Resistance of the electrodes had an average of 1.4 Omega with a standard deviation of +/-0.4 Omega. The surface of nitrocellulose membrane was modified by glutaraldehyde to immobilize streptavidin. Biotinylated anti-mouse IgG was conjugated with polyaniline-coated magnetic nanoparticles. Formation of polyaniline-coated magnetic nanoparticles was confirmed by a transmission electron microscope image. The polyaniline was used as an electric signal transducer for the monitoring of the biospecific binding event. An electrical response induced by the streptavidin-biotin interaction was measured by pulse mode measurement. This measurement method reduced the resistance caused by interfacial capacitance. Dose-dependent resistance changes were also successfully analyzed by the pulse mode polymeric wire biosensor. Results showed that the pulse mode measurement technique enhanced the performance of the polyaniline-based polymeric wire biosensor by reducing the interfacial effects. This approach could be helpful in samples with high interfering background materials, such as food and clinical specimens.
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Jong Seol
authors.initials: JS
authors.lastname: Yuk
authors.forename: Joon-Hyung
authors.initials: JH
authors.lastname: Jin
authors.forename: Evangelyn C
authors.initials: EC
authors.lastname: Alocilja
authors.forename: Joan B
authors.initials: JB
authors.lastname: Rose
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D000814:Aniline Compounds; D007074:Immunoglobulin G; C416807:polyaniline; D019809:Streptavidin