Mineralization of p-nitrophenol by a new isolate Arthrobacter sp. Y1.
Arthrobacter sp. Y1, capable of metabolizing p-nitrophenol (PNP) as the sole carbon, nitrogen and energy source was isolated from activated sludge. The bacterium could tolerate concentrations of PNP up to 600 mg L(- 1), and degradation of PNP was achieved within 120 h of incubation. PNP and its metabolites were analyzed by high performance liquid chromatography (HPLC). The metabolite formed indicated that the organism followed the 4-nitrocathechol (4-NC) pathway for metabolism of this compound. The relevant degrading-enzyme was extracellular. Addition of other carbon source (glucose 0 approximately 30 g L(- 1)) led to accelerated degradation. If the glucose concentration exceeded 30 g L(- 1), however, degradation was repressed. Spectrophotometry assay of the nitrite and genotoxic study showed that strain Y1 could detoxify PNP. Therefore, the present study may provide a basis for the development of the bioremediation strategies to remedy the pollutants in the environment.
Li
Ying Y
YY
College of Life Sciences, Shandong Agricultural University, Shandong, China.
Zhou
Bo
B
Li
Wen
W
Peng
Xiang
X
Zhang
Jing S
JS
Yan
Yan C
YC
D001173:Arthrobacter / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D009596:Nitrophenols / Q000032:analysis* / Q000378:metabolism* / Q000493:pharmacokinetics; D012722:Sewage / Q000737:chemistry / Q000382:microbiology
D009596:Nitrophenols; D012722:Sewage; D005947:Glucose; C024836:4-nitrophenol