Human polymorphonuclear neutrophil responses to Burkholderia pseudomallei in healthy and diabetic subjects.
The major predisposing factor for melioidosis is diabetes mellitus, but no immunological mechanisms have been investigated to explain this. In this study, polymorphonuclear neutrophil (PMN) responses to Burkholderia pseudomallei, the causative agent of melioidosis, in healthy and diabetic Thai subjects were determined by flow cytometry. The results showed that B. pseudomallei displayed reduced uptake by PMNs compared to Salmonella enterica serovar Typhimurium and Escherichia coli. Additionally, intracellular survival of B. pseudomallei was detected throughout a 24-h period, indicating the intrinsic resistance of B. pseudomallei to killing by PMNs. Moreover, PMNs from diabetic subjects displayed impaired phagocytosis of B. pseudomallei, reduced migration in response to interleukin-8, and an inability to delay apoptosis. These data show that B. pseudomallei is intrinsically resistant to phagocytosis and killing by PMNs. These observations, together with the impaired migration and apoptosis in diabetes mellitus, may explain host susceptibility in melioidosis.
Chanchamroen
Sujin
S
Center for Research and Development in Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Kewcharoenwong
Chidchamai
C
Susaengrat
Wattanachai
W
Ato
Manabu
M
Lertmemongkolchai
Ganjana
G
D000328:Adult; D017209:Apoptosis; D016957:Burkholderia pseudomallei / Q000276:immunology*; D054441:Cell Migration Assays, Leukocyte; D002465:Cell Movement; D003920:Diabetes Mellitus / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology; D008297:Male; D050296:Microbial Viability; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis; D012486:Salmonella typhimurium / Q000276:immunology; D013785:Thailand
D016209:Interleukin-8