Porous silicon affinity chips for biomarker detection by MALDI-TOF-MS.
B-type natriuretic peptide (BNP) is an important biomarker in early diagnosis of congestive heart failure. Many efforts have been made previously to evaluate the BNP level in human plasma. We developed a porous silicon (PSi) affinity chip to detect BNP present at low concentrations in human plasma by matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) directly. The PSi surface immobilized with antibodies captured and concentrated BNP through antibody-antigen interaction specifically and sensitively. A detection limit as low as 10 pg/mL BNP in human plasma was demonstrated by mass analysis. This effective on-chip recognition, enrichment, and detection strategy could be employed in identification of biomarkers in complex body fluids in diagnoses.
Chen
Ya-Qing
YQ
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing 210093, Jiangsu, PR China.
Bi
Feng
F
Wang
Shi-Quan
SQ
Xiao
Shou-Jun
SJ
Liu
Jian-Ning
JN
D055910:Antibodies, Immobilized / Q000276:immunology / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers / Q000097:blood*; D002021:Buffers; D006801:Humans; D046208:Microchip Analytical Procedures; D020097:Natriuretic Peptide, Brain / Q000097:blood* / Q000276:immunology; D016062:Porosity; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods
D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D002021:Buffers; D020097:Natriuretic Peptide, Brain; D012825:Silicon