In silico analysis of microsatellites in organellar genomes of major cereals for understanding their phylogenetic relationships.
Microsatellites are abundant across prokaryotic and eukaryotic genomes. However, comparative analysis of microsatellites in the organellar genomes of plants and their utility in understanding phylogeny has not been reported. The purpose of this study was to understand the organization of microsatellites in the coding and non-coding regions of organellar genomes of major cereals viz., rice, wheat, maize and sorghum. About 5.8-14.3% of mitochondrial and 30.5-43.2% of chloroplast microsatellites were observed in the coding regions. About 83.8-86.8% of known mitochondrial genes had at least one microsatellite while this value ranged from 78.6-82.9% among the chloroplast genomes. Dinucleotide repeats were the most abundant in the coding and non-coding regions of the mitochondrial genome while mononucleotides were predominant in chloroplast genomes. Maize harbored more repeats in the mitochondrial genome, which could be due to the larger size of genome. A phylogenetic analysis based on mitochondrial and chloroplast genomic microsatellites revealed that rice and sorghum were closer to each other, while wheat was the farthest and this corroborated with the earlier reported phylogenies based on nuclear genome co-linearity and chloroplast gene-based analysis.
Rajendrakumar
Passoupathy
P
Biotechnology Laboratory, Crop Improvement Section, Directorate of Rice Research, Rajendranagar Hyderabad, India.
Biswal
Akshaya Kumar
AK
Balachandran
Sena M
SM
Sundaram
Raman M
RM
D001483:Base Sequence; D018742:DNA, Chloroplast / Q000032:analysis* / Q000235:genetics; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D002523:Edible Grain / Q000145:classification / Q000235:genetics; D019143:Evolution, Molecular; D018745:Genome, Plant; D018895:Microsatellite Repeats / Q000235:genetics; D012275:Oryza / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D045868:Sorghum / Q000145:classification / Q000235:genetics; D014908:Triticum / Q000145:classification / Q000235:genetics; D003313:Zea mays / Q000145:classification / Q000235:genetics
D018742:DNA, Chloroplast; D004272:DNA, Mitochondrial