Anti-inflammatory effects of Z23 on LPS-induced inflammatory responses in RAW264.7 macrophages.
OBJECTIVE
Fissistigma oldhamii (Hemsl.) Merr, a traditional Chinese herb medicine, is used for treating rheumatoid arthritis in China. In our previous study, an effective compound, 7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl) ethyl] propenamide (Z23), from this herb has showed potent immunosuppressive effects both in vitro and in vivo. However, its anti-inflammatory effect and mechanism is still need to explore.


METHODS
We examined the in vitro effects of Z23 on the production of nitric oxide (NO), prostaglandin E2 (PGE2) and cytokines by lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages.


RESULTS
Z23 significantly decreased the production of PGE2, NO, tumour necrosis factor alpha (TNFalpha) and IL6 production. Inducible nitric oxide synthase (iNOS) and cyclooxygenase2 (COX2) gene expression were also significantly reduced.


CONCLUSIONS
These results demonstrated that Z23 exerted an anti-inflammatory effect through modulating the synthesis of several mediators and cytokines involved in the inflammatory process. This study provided evidence to understand the therapeutic effects of Fissistigma oldhamii (Hemsl.) Merr and indicated that Z23 has the potential for treatment of various inflammatory diseases where the overproduction of NO, PGE2 and inflammatory cytokines has been shown to play a role, e.g. rheumatoid arthritis.
Hu
Xu-Dong
XD
Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
Yang
Yang
Y
Zhong
Xiang-Gen
XG
Zhang
Xiang-Hua
XH
Zhang
Yi-Nan
YN
Zheng
Zong-Ping
ZP
Zhou
Yu
Y
Tang
Wei
W
Yang
Yi-Fu
YF
Hu
Li-Hong
LH
Zuo
Jian-Ping
JP
D000577:Amides / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D029061:Annonaceae / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D015870:Gene Expression; D029001:Herbal Medicine; D015850:Interleukin-6 / Q000378:metabolism*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
C460621:7'-(3',4'-dihydroxyphenyl)-N-((4-methoxyphenyl)ethyl)propenamide; D000577:Amides; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D051546:Cyclooxygenase 2; D015232:Dinoprostone