title: Fluoride release from CaF2 and enamel demineralization.
abstract: The anticaries effect of professional fluoride (F) application has been attributed to calcium-fluoride-like deposits (CaF(2)) formed on enamel, but this has not been clearly demonstrated. We hypothesized that CaF(2) formed on plaque-free enamel by F application would reduce enamel demineralization due to the increase of F availability in fluid of subsequently formed plaque. We created distinct levels of CaF(2) on enamel to evaluate a dose-response effect. Enamel blocks were mounted in contact with a S. mutans test plaque and used in situ by 10 volunteers. F released to the fluid phase of this substrate ("plaque fluid") was measured before a cariogenic challenge. "Plaque fluid" F concentration was highly correlated to the enamel CaF(2) concentration (r = 0.96, p < 0.001) and to consequent enamel demineralization (r = -0.75, p < 0.001). The results suggest that F released to plaque fluid from CaF(2) formed on enamel may play a significant role in the anticaries effect of professionally applied F agents.
authors.affiliation: Piracicaba Dental School, UNICAMP, Av. Limeira 901, 13414-903, Piracicaba, SP Piracicaba, SP, Brazil. litenuta@fop.unicamp.br
authors.forename: L M A
authors.initials: LM
authors.lastname: Tenuta
authors.forename: R V
authors.initials: RV
authors.lastname: Cerezetti
authors.forename: A A
authors.initials: AA
authors.lastname: Del Bel Cury
authors.forename: C P M
authors.initials: CP
authors.lastname: Tabchoury
authors.forename: J A
authors.initials: JA
authors.lastname: Cury
mesh_terms: D000149:Acidulated Phosphate Fluoride / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000818:Animals; D001031:Apatites / Q000378:metabolism; D001682:Biological Availability; D002124:Calcium Fluoride / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002327:Cariostatic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002417:Cattle; D018592:Cross-Over Studies; D003743:Dental Enamel / Q000378:metabolism*; D003773:Dental Plaque / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D005460:Fluorides, Topical / Q000494:pharmacology*; D006244:Hardness; D006801:Humans; D017001:Tooth Demineralization / Q000517:prevention & control*
chemical_list: D001031:Apatites; D002327:Cariostatic Agents; D005460:Fluorides, Topical; D000149:Acidulated Phosphate Fluoride; C025105:fluorapatite; D002124:Calcium Fluoride