Adhesion of Campylobacter jejuni and Mycobacterium avium onto polyethylene terephtalate (PET) used for bottled waters.
Adhesion of the bacteria Campylobacter jejuni and Mycobacterium avium onto polyethylene terephtalate (PET), a polymer widely used within the bottled water industry was measured in two different groundwater solutions. From this, it was found that whilst the percentage cell adhesion for a given strain did not change between groundwater types, substantial variation was obtained between the two bacterial species tested: M. avium (10-30% adhered cells) and C. jejuni (1-2%) and no major variations were measured as a function of groundwater composition for a given strain. To explain this, the interfacial electro-hydrodynamic properties of the bacteria were investigated by microelectrophoresis, with the resultant data analysed on the basis of electrokinetic theory for soft biocolloidal particles. The results obtained showed that M. avium carries a significant volume charge density and that its peripheral layer exhibits limited hydrodynamic flow permeation compared to that of C. jejuni. It was also demonstrated that steric hindrance to flow penetration and the degree of hydrophobicity within/of the outer bacterial interface are larger for M. avium cells. In line with this, the larger amount of M. avium cells deposited onto PET substrates as compared to that of C. jejuni can be explained by hydrophobic attraction and chemical binding between hydrophobic PET and outer soft surface layer of the bacteria. Hydrophobicity of PET was addressed by combining contact angle analyses and force spectroscopy using CH(3)-terminated AFM tip.
Tatchou-Nyamsi-König
Josiane-Aurore
JA
Laboratory of Physical Chemistry and Microbiology for the Environment, Nancy-University, CNRS, 405 rue de Vandoeuvre, F-54600 Villers-lès-Nancy, France.
Dague
Etienne
E
Mullet
Martine
M
Duval
Jérôme F L
JF
Gaboriaud
Fabien
F
Block
Jean-Claude
JC
D001422:Bacterial Adhesion; D016123:Campylobacter jejuni / Q000502:physiology*; D024202:Electrophoretic Mobility Shift Assay; D018625:Microscopy, Atomic Force; D009162:Mycobacterium avium / Q000502:physiology*; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D014881:Water Supply
D011093:Polyethylene Terephthalates