Detection of heteromers formed by cannabinoid CB1, dopamine D2, and adenosine A2A G-protein-coupled receptors by combining bimolecular fluorescence complementation and bioluminescence energy transfer.
Functional interactions in signaling occur between dopamine D2 (D2R) and cannabinoid CB1 (CB1R) receptors, between CB1R and adenosine A2A (A2AR) receptors, and between D2R and A2AR. Furthermore, direct molecular interactions have been reported for the pairs CB1R-D2R, A2AR-D2R, and CB1R-A2AR. Here a combination of bimolecular fluorescence complementation and bioluminescence energy transfer techniques was used to identify the occurrence of D2R-CB1R-A2AR hetero-oligomers in living cells.
Navarro
Gemma
G
Institut d'Investigacions Biomèdiques August Pi i Sunyer Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, y Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universitat de Barcelona, Spain.
Carriba
Paulina
P
Gandía
Jorge
J
Ciruela
Francisco
F
Casadó
Vicent
V
Cortés
Antoni
A
Mallol
Josefa
J
Canela
Enric I
EI
Lluis
Carmen
C
Franco
Rafael
R
D002460:Cell Line; D019281:Dimerization; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007668:Kidney / Q000196:embryology; D008163:Luminescent Measurements; D020836:Protein Structure, Quaternary; D043705:Receptor, Adenosine A2A / Q000378:metabolism*; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism*; D017448:Receptors, Dopamine D2 / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection
D043705:Receptor, Adenosine A2A; D043884:Receptor, Cannabinoid, CB1; D017448:Receptors, Dopamine D2; D011993:Recombinant Fusion Proteins