title: Sequence and analysis of a plasmid-encoded mercury resistance operon from Mycobacterium marinum identifies MerH, a new mercuric ion transporter.
abstract: In this study, we report the DNA sequence and biological analysis of a mycobacterial mercury resistance operon encoding a novel Hg(2+) transporter. MerH was found to transport mercuric ions in Escherichia coli via a pair of essential cysteine residues but only when coexpressed with the mercuric reductase.
authors.affiliation: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom. matschue@yahoo.fr
authors.forename: Mathieu
authors.initials: M
authors.lastname: Schué
authors.forename: Lynn G
authors.initials: LG
authors.lastname: Dover
authors.forename: Gurdyal S
authors.initials: GS
authors.lastname: Besra
authors.forename: Julian
authors.initials: J
authors.lastname: Parkhill
authors.forename: Nigel L
authors.initials: NL
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D017124:Conserved Sequence; D017931:DNA Primers; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D017669:Mercury Compounds / Q000378:metabolism* / Q000494:pharmacology*; D008969:Molecular Sequence Data; D019910:Mycobacterium marinum / Q000187:drug effects* / Q000235:genetics*; D009876:Operon / Q000235:genetics*; D010957:Plasmids; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D017931:DNA Primers; D017669:Mercury Compounds