title: Differential effects of five Aggregatibacter actinomycetemcomitans strains on gingival epithelial cells.
abstract: BACKGROUND
  We investigated gingival epithelial cell proliferation and expression of interleukin-8 (IL-8) and intercellular adhesion molecule 1 (ICAM-1) in response to Aggregatibacter actinomycetemcomitans serotypes a, b, and c.
  
  
  METHODS
  Human gingival cells (Ca9-22) were cultured in bacterial extracts prepared from five strains of A. actinomycetemcomitans: ATCC 43717 (serotype a); ATCC 29524, ATCC 29522, and ATCC 43718 (all serotype b); and ATCC 43719 (serotype c).
  
  
  RESULTS
  In bacterial extracts of ATCC 29522, cell growth was significantly impaired, while the expression of IL-8 and ICAM-1 was significantly increased. The level of induction in response to the other strains was minimal.
  
  
  CONCLUSIONS
  Our results indicate that the five strains of A. actinomycetemcomitans have distinct effects on the abilities of human gingival epithelial cells to proliferate and to produce proinflammatory factors.
authors.affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shimada
authors.forename: N
authors.initials: N
authors.lastname: Sugano
authors.forename: R
authors.initials: R
authors.lastname: Nishihara
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Ito
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology*; D016976:Aggregatibacter actinomycetemcomitans / Q000472:pathogenicity*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D005881:Gingiva / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013045:Species Specificity
chemical_list: D016209:Interleukin-8; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1