title: Microarray analysis of micro-ribonucleic acid expression in primary immunoglobulin A nephropathy.
abstract: OBJECTIVE
  To explore the relationship between immunoglobulin A nephropathy (IgAN) and microRNA (miRNA).
  
  
  METHODS
  We analyzed the miRNA expression profiles in renal biopsies from 11 IgAN patients and 3 controls at the Kidney Transplantation and Hemo Purification Center of 181 Hospital, China, from May to October 2007, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences.
  
  
  RESULTS
  This study identified 132 miRNAs in renal samples, of which 35 miRNAs up-regulated in IgAN biopsies. The chip results were confirmed by northern blot analysis and by quantitative real-time polymerase chain reaction (RT-PCR) tests.
  
  
  CONCLUSIONS
  Our study may help clarify the molecular mechanisms involved in the pathogenesis of IgAN, and miRNAs potentially serve as a novel diagnostic biomarker of IgAN.
authors.affiliation: Clinical Medical Research Center, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Dai
authors.forename: Weiguo
authors.initials: W
authors.lastname: Sui
authors.forename: Huijuan
authors.initials: H
authors.lastname: Lan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yan
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Yuanshuai
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D044466:Asian People / Q000235:genetics; D001706:Biopsy; D016022:Case-Control Studies; D002681:China; D005260:Female; D005922:Glomerulonephritis, IGA / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007668:Kidney / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D035683:MicroRNAs