title: High-efficiency liquid chromatographic separation utilizing long monolithic silica capillary columns.
abstract: Long monolithic silica-C18 capillary columns of 100 microm i.d. were prepared, and the efficiency was examined using reversed-phase HPLC under a pressure of up to 47 MPa. At linear velocities of 1-2 mm/s, 100,000-500,000 theoretical plates could be generated with a single column (90-440 cm in length) using an acetonitrile-water (80/20) mobile phase with a column dead time (t0) of 5-40 min. It was possible to prepare columns with a minimum plate height of 8.5 +/- 0.5 microm and permeability of (1.45 +/- 0.09) x 10(-13) m(2). The chromatographic performance of a long octadecylsilylated monolithic silica capillary column was demonstrated by the high-efficiency separations of aromatic hydrocarbons, benzene derivatives, and a protein digest. The efficiency for a peptide was maintained for an injection of up to 0.5-2 ng. When three 100 microm i.d. columns were connected to form a 1130-1240 cm column system, 1,000,000 theoretical plates were generated for aromatic hydrocarbons with retention factors of up to 2.4 with a t0 of 150 min. The fact that very high efficiencies were obtained for the retained solutes suggests the practical utility of these long monolithic silica capillary columns.
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Hironobu
authors.initials: H
authors.lastname: Morisaka
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tokuda
authors.forename: Kanta
authors.initials: K
authors.lastname: Horie
authors.forename: Kodai
authors.initials: K
authors.lastname: Koduki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Makino
authors.forename: Oscar
authors.initials: O
authors.lastname: Núñez
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Takefumi
authors.initials: T
authors.lastname: Kawabe
authors.forename: Tohru
authors.initials: T
authors.lastname: Ikegami
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Takubo
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ishihama
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006841:Hydrocarbons, Aromatic / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism; D012822:Silicon Dioxide
chemical_list: D006841:Hydrocarbons, Aromatic; D010455:Peptides; D012822:Silicon Dioxide