title: The heterotrimeric Galpha protein pga1 regulates biosynthesis of penicillin, chrysogenin and roquefortine in Penicillium chrysogenum.
abstract: We have studied the role of the pga1 gene of Penicillium chrysogenum, encoding the alpha subunit of a heterotrimeric G protein, in secondary metabolite production. The dominant activating pga1(G42R) mutation caused an increase in the production of the three secondary metabolites penicillin, the yellow pigment chrysogenin and the mycotoxin roquefortine, whereas the dominant inactivating pga1(G203R) allele and the deletion of the pga1 gene resulted in a decrease of the amount of produced penicillin and roquefortine. Chrysogenin is produced in solid medium as a yellow pigment, and its biosynthesis is clearly enhanced by the presence of the dominant activating pga1(G42R) allele. Roquefortine is produced associated with mycelium during the first 3 days in submerged cultures, and is released to the medium afterwards; dominant activating and inactivating pga1 mutations result in upregulation and downregulation of roquefortine biosynthesis recpectively. Pga1 regulates penicillin biosynthesis by controlling expression of the penicillin biosynthetic genes; the three genes pcbAB, pcbC and penDE showed elevated transcript levels in transformants expressing the pga1(G42R) allele, whereas in transformants with the inactivating pga1(G203R) allele and in the pga1-deleted mutant their transcript levels were lower than those in the parental strains. Increase of intracellular cAMP levels had no effect on penicillin production. In summary, the dominant activating pga1(G42R) allele upregulates the biosynthesis of three secondary metabolites in Penicillium chrysogenum to a different extent.
authors.affiliation: Área de Microbiología, Fac. CC. Biológicas y Ambientales, Universidad de León, León 24071, Spain.|Instituto de Biotecnología de León, INBIOTEC, Parque Científico de León, León 24006, Spain.
authors.forename: Ramón O
authors.initials: RO
authors.lastname: García-Rico
authors.affiliation: Área de Microbiología, Fac. CC. Biológicas y Ambientales, Universidad de León, León 24071, Spain.|Instituto de Biotecnología de León, INBIOTEC, Parque Científico de León, León 24006, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Fierro
authors.affiliation: Instituto de Biotecnología de León, INBIOTEC, Parque Científico de León, León 24006, Spain.
authors.forename: Elba
authors.initials: E
authors.lastname: Mauriz
authors.affiliation: Instituto de Biotecnología de León, INBIOTEC, Parque Científico de León, León 24006, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Gómez
authors.affiliation: Instituto de Biotecnología de León, INBIOTEC, Parque Científico de León, León 24006, Spain.
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Fernández-Bodega
authors.affiliation: Área de Microbiología, Fac. CC. Biológicas y Ambientales, Universidad de León, León 24071, Spain.|Instituto de Biotecnología de León, INBIOTEC, Parque Científico de León, León 24006, Spain.
authors.forename: Juan F
authors.initials: JF
authors.lastname: Martín
mesh_terms: D000242:Cyclic AMP / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006576:Heterocyclic Compounds, 4 or More Rings / Q000378:metabolism; D007211:Indoles / Q000378:metabolism*; D009154:Mutation; D010406:Penicillins / Q000096:biosynthesis*; D010408:Penicillium chrysogenum / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010860:Pigments, Biological / Q000096:biosynthesis*; D010879:Piperazines / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D006576:Heterocyclic Compounds, 4 or More Rings; D007211:Indoles; D010406:Penicillins; D010860:Pigments, Biological; D010879:Piperazines; D021122:Protein Subunits; C012536:roquefortine; D000242:Cyclic AMP