[Detection of Helicobacter pylori genotypes from human gastric mucosa and its association with gastric diseases].
OBJECTIVE
To investigate the feasibility of detecting Helicobacter pylori (Hp) directly from gastric mucosa and the relationship of HP genotypes to gastric diseases.


METHODS
Specimens of gastric mucosa were collected by biopsy from 217 patients, 90 with superficial gastritis (GS), 70 with atrophic gastritis (GA), 28 with gastroesophageal erosions and ulcers (GEU), and 29 with gastric cancer (GC), to undergo pathological examination, culture of Hp, and DNA isolation from the gastric mucosa respectively. Routine phenol/chloroform method was used to isolate the DNA in the cultured Hp. PCR was conducted to detect the ureB, cagA, vacAs1, vacAm1, vacAm2, iceA1, iceA2, and baba2 genotypes in both the gastric mucosa-originated Hp-DNA and cultured Hp-DNA.


RESULTS
The concordance rates of ureB, cagA, vacAs1, vacAm1, vacAm2, iceA1, iceA2, and baba2 genotypes between the gastric mucosa-originated Hp-DNA and cultured Hp-DNA were 74.23%, 73.39%, 93.69%, 62.16%, 78.16%, 89.13%, 88.37%, and 75% respectively (all P > 0.05). There were no significant differences in the distribution rates of ureB, cagA, vacAs1, vacAm1b, iceA1, iceA2, and baba2 genotypes among different gastric diseases (all P > 0.05). However, the distribution frequency of vacAs1m1 strain in the GA patients was 22.22%, significantly higher than that in the GA patients (5.71%, chi2 = 8.416, P = 0.004), and the distribution frequency of vacAs1m2 strains in the GA patients was 47.14%, significantly higher than that in GS patients (18.89%, chi2 = 13.336, P = 0.000).


CONCLUSIONS
Rapid detection of Hp can be achieved by using DNA isolated directly from infected gastric mucosa. VacAs1 m1 is associated with GA and vacAs1m2 is associated with GA.
Liu
Yun-en
YE
Cancer Institute, First Affiliated Hospital, China Medical University, Shenyang.
Gong
Yue-hua
YH
Sun
Li-ping
LP
Wang
Yan-li
YL
Chen
Tie-jun
TJ
Yuan
Yuan
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology*; D005756:Gastritis / Q000382:microbiology*; D005757:Gastritis, Atrophic / Q000382:microbiology; D005787:Gene Frequency; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000382:microbiology; D055815:Young Adult
D001426:Bacterial Proteins; D004269:DNA, Bacterial