A bulge binding agent with novel wedge-shape topology for stimulation of DNA triplet repeat strand slippage synthesis.
Expansion of DNA repeat sequences is associated with many human genetic diseases. Bulged DNA structures have been implicated as intermediates in DNA slippage within the DNA repeat regions. Herein a bulge binding agent with novel wedge-shape topology of the aromatic moiety was designed and synthesized. The compound-bulge DNA interactions were characterized via UV melting experiments, circular dichroism and were quantitated by surface plasmon resonance with K(d) of 41.5 microM. This compound showed remarkable stimulation for DNA triplet repeat strand slippage synthesis in vitro.
Liu
Liangliang
L
State Key Laboratory of Elemento-Organic Chemistry, Department of Chemical Biology, Nankai University, 94 Weijin Road, Tianjin 300071, China.
Li
Heyang
H
Yi
Long
L
Yang
Xing
X
Wen
Xin
X
Xi
Zhen
Z
D000606:Amino Sugars / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D015394:Molecular Structure; D010640:Phenothiazines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D018911:Trinucleotide Repeats / Q000187:drug effects
D000606:Amino Sugars; D010640:Phenothiazines; D004247:DNA; C031637:phenothiazine