title: Enhanced photo-H2 production of R. faecalis RLD-53 by separation of CO2 from reaction system.
abstract: The effect of different gases, CO(2) concentration, and separation of CO(2) from reaction system on photo-fermentation H(2) production was investigated by batch culture in this study. Experimental results showed that different gases (Ar,N(2),CO(2), and air) as gas phase have obviously affected on photo-H(2) production and a high concentration of CO(2) can inhibit the growth and H(2) evolution of Rhodopseudomonas faecalis RLD-53. When CO(2) concentration at 5%, cell increased most rapidly the specific growth rate of 0.489 g/l/h and the specific growth rate fell to be 0.265 g/l/h when CO(2) concentration at 40%. However, the growth of RLD-53 at CO(2) concentration of 60-100% was almost completely inhibited. At CO(2) concentrations of 5% and 10%, the maximum H(2) yield was 2.54 and 2.59 mol-H(2)/mol acetate, respectively, and it was similar with the control (2.61 mol-H(2)/mol acetate). H(2) not produced when CO(2) concentration at 60-100%. In conclusion, separation of CO(2) from reaction system can stimulate H(2) production in the entire photo-H(2) production process and H(2) yield increased about 12.8-18.85% than the control.
authors.affiliation: State Key Lab of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Bing-Feng
authors.initials: BF
authors.lastname: Liu
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Xie
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Cao
mesh_terms: D002245:Carbon Dioxide / Q000302:isolation & purification* / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D008027:Light; D018462:Photobiology / Q000379:methods; D012241:Rhodopseudomonas / Q000502:physiology* / Q000528:radiation effects*
chemical_list: D002245:Carbon Dioxide; D006859:Hydrogen