Element-tagged immunoassay with inductively coupled plasma mass spectrometry for multianalyte detection.
The multianalyte immunoassay approach is currently attracting increasing attention due to its high sample throughput, short assay time, low sample consumption and reduced overall cost per assay. This paper reviews progress in the field of multianalyte immunoassays using inductively coupled plasma mass spectrometry, as well as applications of this approach in different fields. Examples related to the combination of protein microarray technology with the multitag approach of the immunoassay ICP-MS method and to the use of ICP-MS in the field of imaging are described. A novel strategy that involves tagging antibodies for ICP-MS detection in sensitive multitag bioassays is also presented. Finally, the outlook for this promising technique is discussed.
Careri
Maria
M
Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università di Parma, Viale G.P. Usberti 17/A, 43100, Parma, Italy. careri@unipr.it
Elviri
Lisa
L
Mangia
Alessandro
A
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry; D040081:Protein Array Analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000906:Antibodies; D002614:Chelating Agents; D009942:Organometallic Compounds