Effect of 60 Hz electromagnetic fields on the activity of hsp70 promoter: an in vitro study.
We have evaluated the effect of 60 Hz sinusoidal magnetic fields (MF) at 8 and 8 microT on expression of the luciferase gene contained in a gene construct labelled as Electromagnetic Field-plasmid (pEMF). The vector included the hsp70 promotor containing the 3 nCTCTn sequences previously described for the induction of hsp70 expression by magnetic fields, as well as the reporter of the luciferase gene. We also replicated the study of Lin et al. [Lin H, Blank M, Rossol-Haseroth K, Goodman R. Regulating genes with electromagnetic response elements. J Cell Biochem 2001;81(1):143-48]. The pEMF plasmid was transfected into HeLa and BMK16 cell lines that were later exposed to either MF or thermal shock (TS). An increased luciferase expression was found in both the cells exposed to MF and TS compared with their control groups (P < 0.05). Furthermore, the combined effect of MF and TS was also analyzed. A synergistic effect between two factors was observed for this co-exposure condition in terms of luciferase gene expression.
Rodríguez de la Fuente
Abraham O
AO
Departamento de Ciencias Exactas y Desarrollo Humano, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico. abrrodriguez@fcb.uanl.mx
Alcocer-González
Juan M
JM
Antonio Heredia-Rojas
J
J
Balderas-Candanosa
Isaías
I
Rodríguez-Flores
Laura E
LE
Rodríguez-Padilla
Cristina
C
Taméz-Guerra
Reyes S
RS
D000818:Animals; D045744:Cell Line, Tumor; D004574:Electromagnetic Fields; D015870:Gene Expression; D017930:Genes, Reporter; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis; D051379:Mice; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D011994:Recombinant Proteins; D008156:Luciferases