title: Molecular typing of Mycobacterium chelonae isolates from a pseudo-outbreak involving an automated bronchoscope washer.
abstract: We describe a pseudo-outbreak of Mycobacterium chelonae infection in bronchoalveolar lavage fluid from 9 patients that was traced to contamination of an automated bronchoscope washer. Molecular typing using repetitive extragenic palindromic polymerase chain reaction was helpful in confirming epidemiologic and clinical findings.
authors.affiliation: Department of Pulmonary and Critical Care Medicine, Lahey Clinic Medical Center, Burlington, Massachusetts 01805, USA.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Chroneou
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Zimmerman
authors.forename: Steven
authors.initials: S
authors.lastname: Cook
authors.forename: Sandra
authors.initials: S
authors.lastname: Willey
authors.forename: Jane
authors.initials: J
authors.lastname: Eyre-Kelly
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Zias
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Shapiro
authors.forename: John F
authors.initials: JF
authors.lastname: Beamis
authors.forename: Donald E
authors.initials: DE
authors.lastname: Craven
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015373:Bacterial Typing Techniques; D020680:Bronchoscopes / Q000382:microbiology*; D003428:Cross Infection / Q000209:etiology / Q000382:microbiology*; D004196:Disease Outbreaks; D004203:Disinfection / Q000295:instrumentation / Q000379:methods; D004866:Equipment Contamination; D006801:Humans; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology*; D016926:Mycobacterium chelonae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification