Safety evaluation of a canine hepatitis DNA vaccine.
DNA vaccines are a novel disease prevention methodology, however their safety has not been well described. We evaluated the safety and efficacy of the DNA vaccine pVAX1-CpG-Loop against infectious canine hepatitis. We demonstrated that pVAX1-CpG-Loop could not be recovered from tissues of vaccinated mice nor from F1 progeny and following vaccination there were no apparent changes in hematologic markers compared to unvaccinated controls. Most important, vaccinated mice were protected from viral challenge. The only detectable effects of the vaccination were elevated AST levels 4 weeks post vaccination and liver lymphocyte infiltration and hydropic degeneration which normalized 6 months later.
Liu
Fu Ying
FY
Department of Molecular Biology, Hebei Key Lab of Laboratory Animal, Hebei Medical University, Shijiazhuang 050017, People's Republic of China.
Guo
Rui Min
RM
Zheng
Long
L
Lu
Yi Ming
YM
You
Hongyu
H
Shuxia
Song
S
Wang
Jun Xia
JX
Sun
Shu Han
SH
D006516:Adenoviruses, Canine / Q000276:immunology*; D000818:Animals; D017931:DNA Primers / Q000276:immunology; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D004285:Dogs; D005260:Female; D018095:Germ-Line Mutation; D006522:Hepatitis, Infectious Canine / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D007115:Immunization Schedule; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D019444:Vaccines, DNA / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000009:adverse effects / Q000276:immunology* / Q000627:therapeutic use*
D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines