Determination of metaldehyde in human serum by headspace solid-phase microextraction and gas chromatography-mass spectrometry.
A rapid headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) method has been developed for the determination of metaldehyde in human serum samples. Metaldehyde is extensively used as a molluscicide for the control of slugs and snails, and cases of metaldehyde poisoning have been reported. Metaldehyde was headspace-extracted on a polydimethylsiloxane (PDMS) fiber at 70 degrees C for 25 min, desorbed, and analyzed rapidly by GC-MS. The method was validated for limit of detection (LOD), linearity, precision, and recovery. Although the recovery of the sample was very low, the method itself was rapid with a low detection limit of 0.25 microg/ml, R.S.D. value 12.6%, and linearity range 0.5-25.0 microg/ml (r(2)=0.999). The results demonstrated that the SPME-GC-MS method for the analysis of metaldehyde is simple, rapid, solvent-free, and does not require any pre-analysis conversions.
Saito
Takeshi
T
Department of Emergency and Clinical Care Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1143, Japan. saito@is.icc.u-tokai.ac.jp
Morita
Seiji
S
Motojyuku
Megumi
M
Akieda
Kazuki
K
Otsuka
Hiroyuki
H
Yamamoto
Isotoshi
I
Inokuchi
Sadaki
S
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000097:blood / Q000506:poisoning; D004129:Dimethylpolysiloxanes; D004355:Drug Stability; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008975:Molluscacides / Q000097:blood / Q000506:poisoning; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*; D013406:Suicide, Attempted; D055815:Young Adult
D004129:Dimethylpolysiloxanes; D008975:Molluscacides; C002935:metaldehyde; C013830:baysilon; D000079:Acetaldehyde