The development of peptide-based interfacial biomaterials for generating biological functionality on the surface of bioinert materials.
Biomaterials used in implants have traditionally been selected based on their mechanical properties, chemical stability, and biocompatibility. However, the durability and clinical efficacy of implantable biomedical devices remain limited in part due to the absence of appropriate biological interactions at the implant interface and the lack of integration into adjacent tissues. Herein, we describe a robust peptide-based coating technology capable of modifying the surface of existing biomaterials and medical devices through the non-covalent binding of modular biofunctional peptides. These peptides contain at least one material binding sequence and at least one biologically active sequence and thus are termed, "Interfacial Biomaterials" (IFBMs). IFBMs can simultaneously bind the biomaterial surface while endowing it with desired biological functionalities at the interface between the material and biological realms. We demonstrate the capabilities of model IFBMs to convert native polystyrene, a bioinert surface, into a bioactive surface that can support a range of cell activities. We further distinguish between simple cell attachment with insufficient integrin interactions, which in some cases can adversely impact downstream biology, versus biologically appropriate adhesion, cell spreading, and cell survival mediated by IFBMs. Moreover, we show that we can use the coating technology to create spatially resolved patterns of fluorophores and cells on substrates and that these patterns retain their borders in culture.
Meyers
Steven R
SR
Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, MA 02215, USA.
Khoo
Xiaojuan
X
Huang
Xin
X
Walsh
Elisabeth B
EB
Grinstaff
Mark W
MW
Kenan
Daniel J
DJ
D000595:Amino Acid Sequence; D017209:Apoptosis; D001672:Biocompatible Materials / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011137:Polystyrenes / Q000378:metabolism*; D013499:Surface Properties
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D010455:Peptides; D011137:Polystyrenes; D001710:Biotin; D016650:Fluorescein-5-isothiocyanate