A high-throughput assay for siRNA-based circadian screens in human U2OS cells.
The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells.
Vollmers
Christopher
C
Regulatory Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, United States of America.
Panda
Satchidananda
S
DiTacchio
Luciano
L
D002460:Cell Line; D002940:Circadian Rhythm / Q000235:genetics*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D020868:Gene Silencing; D017930:Genes, Reporter; D006801:Humans; D008163:Luminescent Measurements; D008722:Methods; D034741:RNA, Small Interfering / Q000494:pharmacology*
D034741:RNA, Small Interfering