title: Ultrastructural and Immunochemical Changes During the In Vitro Development of Plasmopara halstedii.
abstract: ABSTRACT The asexual phase of the life cycle of Plasmopara halstedii, the causal agent of downy mildew of sunflower, plays a key role in the propagation of the disease. We investigated the morphological and ultrastructural changes that occur during the asexual development of the pathogen. Direct examination of infected cotyledons confirmed the presence of sporangiophores. In contact with water, important ultrastructural changes occurred, affecting the surface of zoosporangia, which became smoother, and their cytoplasm, which differentiated into flagellate zoospores. The subsequent encystment of zoospores was characterized by the synthesis of a cell wall and the loss of the flagella. In addition, two monoclonal antibodies (MAbs) specific for P. halstedii were used to analyze the immunochemical changes associated with these modifications. MAb 16A6, which bound to a 48-kDa glycoprotein, mainly labeled the surface of mobile or encysted zoospores and of mother cells of germ tubes. Conversely, MAb 2F9, which recognized highly glycosylated antigens, labeled the surface of zoosporangia and of flagellate zoospores, but not the encysted zoospores. These results provide new insights into the morphological and ultrastructural changes associated with the release and the encystment of zoospores which may be interesting targets for the development of new antimicrobial products.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bouterige
authors.forename: Guy
authors.initials: G
authors.lastname: Tronchin
authors.forename: Maurice
authors.initials: M
authors.lastname: Lesourd
authors.forename: Agnès
authors.initials: A
authors.lastname: Marot-Leblond
authors.forename: Valérie
authors.initials: V
authors.lastname: Molinéro
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Bouchara
authors.forename: Raymond
authors.initials: R
authors.lastname: Robert