Vitamin E supplementation in rats with experimental diabetes mellitus: analysis of myosin-V and nNOS immunoreactive myenteric neurons from terminal ileum.
The effect of vitamin E (1 g/kg body weight) supplementation on myosin-V and neuronal nitric oxide synthase (nNOS) immunoreactive myenteric neurons from the ileum of diabetic rats was investigated in the present study. Forty animals were divided into the following groups: normoglycemics (N), normoglycemics treated with vitamin E (NE), diabetics (D), and diabetics treated with vitamin E (DE). Quantitative and morphometric analyses were performed. The area of the tertiary plexus was also determined. Diabetes produced a 24% reduction in the number of myosin-V neurons in group D compared with group N, an effect that was accompanied by an increase in the tertiary plexus area (P < 0.05). Neuronal density was 27% higher in group NE than group N (P < 0.05). Nitrergic neuronal density was not altered as a consequence of either diabetes or vitamin E treatment. Myosin-V and nNOS immunoreactive neuronal cell body area increased significantly in group NE. The area of myosin-V and nNOS myenteric neurons also increased in group D. Vitamin E treatment (group DE) increased only the size of nitrergic neurons. The present results suggest that vitamin E elicited a neuroprotective and neurotrophic effect on the natural aging process, but with regard to diabetes, vitamin E supplementation exerted a neurotrophic effect only on nitrergic neurons.
Pereira
Renata Virginia Fernandes
RV
Department of Morphophysiological Sciences, Universidade Estadual de Maringá, Avenida Colombo, no. 5790 Bloco H-79, CEP 87020-900 Maringá, PR, Brazil.
de Miranda-Neto
Marcílio Hubner
MH
da Silva Souza
Ivan Domicio
ID
Zanoni
Jacqueline Nelisis
JN
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D019587:Dietary Supplements; D006801:Humans; D007082:Ileum / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology; D024701:Myosin Type V / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000008:administration & dosage; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000008:administration & dosage*
D000975:Antioxidants; D018696:Neuroprotective Agents; D014810:Vitamin E; D052248:Nitric Oxide Synthase Type I; D024701:Myosin Type V