title: [Molecular mechanism underlying differentiation of HL-60 cells induced by hexamethylene bisacetamide].
abstract: The objective of this study was to investigate the effect of hexamethylene bisacetamide (HMBA) on differentiation of HL-60 cells and its possible molecular mechanism. HL-60 cells were co-cultured with different concentrations of HMBA (0.5, 1, 2 mmol/L) for 4 days, then the proliferation was assayed by MTT at different time points. Wright-Giemsa staining was used to observe the change in morphology. Cell differentiation antigen CD11b expression and the altered distribution of cell cycle in HL-60 induced by HMBA were analyzed by flow cytometry. The expressions of c-myc, mad1, p21, p27, hTERT and HDAC1 mRNA were detected by RT-PCR. The results indicated that the proliferation of HL-60 cells was inhibited by HMBA in a time-and-dose-dependent manner. Upon 2 mmol/L HMBA treatment, the HL-60 cells arrested at G(0)/G(1) phase and differentiated into granular line in morphology, with the up-regulation of CD11b expression. The expression of c-myc and hTERT mRNA obviously down-regulated, the expression of p21, p27 and mad1 mRNA up-regulated, while there was no change of the expression of hTERT mRNA. It is concluded that effect of HMBA on the differentiation of HL-60 cells may partly contribute to switch from c-myc to mad1 expression, to up-regulate expressions of p21 and p27 mRNA, and down-regulate hTERT mRNA expression, while there is no relation with the expression of HDAC1 mRNA.
authors.affiliation: Institute of Basic Medical Sciences, Shandong Academy of Medical Sciences, Jinan 250062, Shangdong Province, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Ren
authors.forename: Pei-E
authors.initials: PE
authors.lastname: Wen
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Yang
authors.forename: Tian-Hua
authors.initials: TH
authors.lastname: Tang
authors.forename: Hai-Quan
authors.initials: HQ
authors.lastname: Ren
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Zhao
authors.forename: Hua
authors.initials: H
authors.lastname: Fan
authors.forename: Gao-Juan
authors.initials: GJ
authors.lastname: Qiao
authors.forename: Guo-Heng
authors.initials: GH
authors.lastname: Jiang
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans
chemical_list: D000081:Acetamides; D000970:Antineoplastic Agents; C014026:hexamethylene bisacetamide