Single- and joint-population analyses of two experimental pig crosses to confirm quantitative trait loci on Sus scrofa chromosome 6 and leptin receptor effects on fatness and growth traits.
The primary goal of this study was to detect and confirm QTL on SSC6 for growth and fatness traits in 2 experimental F(2) intercrosses: Iberian x Landrace (IB x LR) and Iberian x Meishan (IB x MS), which were used in this study for the first time in a QTL analysis related to productive traits. For this purpose, single- and joint-population analyses with single and bivariate trait models of both populations were performed. The presence of the SSC6 QTL for backfat thickness previously identified in the IB x LR cross was detected in this population with additional molecular information, but also was confirmed in the IB x MS cross. In addition, a QTL affecting BW was detected in both crosses in a similar position to the QTL detected for backfat thickness. This is the first study in which a QTL affecting BW is detected on SSC6 in the IB x LR cross, as well as in the IB x MS resource population. Furthermore, we analyzed a previously described nonsynonymous leptin receptor (LEPR) SNP located in exon 14 (c.2002C > T) for causality with respect to this QTL within both F(2) populations. Our results supported the previously reported association between LEPR alleles and backfat thickness in the IB x LR cross, and this association was also confirmed within the IB x MS cross. An association not reported before between LEPR alleles and BW was identified in both populations.
Muñoz
G
G
Departamento de Mejora Genética Animal, Subdirección General de Investigación y Tecnología-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040 Madrid, Spain. munoz.gloria@inia.es
Ovilo
C
C
Silió
L
L
Tomás
A
A
Noguera
J L
JL
Rodríguez
M C
MC
D000273:Adipose Tissue / Q000254:growth & development*; D000818:Animals; D001947:Breeding; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006239:Haplotypes; D008297:Male; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci / Q000235:genetics*; D054411:Receptors, Leptin / Q000235:genetics*; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development*
D005819:Genetic Markers; D054411:Receptors, Leptin