title: Effects of inoculum, delayed loading, and storage temperature of blood culture bottles on the detection of Burkholderia pseudomallei.
abstract: Detection of Burkholderia pseudomallei in blood culture is critical to prompt appropriate treatment. Various factors may affect culture positivity. This study evaluated effects of various inocula (0.2-100 CFU/mL), delayed bottle loading periods (12-48 h), and storage temperatures (ambient, 4 and 35 degrees C) for the detection of B. pseudomallei by the BacT/ALERT system. The results suggested that bottles may be preincubated at 35 degrees C for up to 12 h, or held at ambient temperature for up to 24 h, or refrigerated at 4 degrees C for up to 48 h.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. sipkr@mahidol.ac.th
authors.forename: Pattarachai
authors.initials: P
authors.lastname: Kiratisin
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D016957:Burkholderia pseudomallei / Q000302:isolation & purification*; D006801:Humans; D008554:Melioidosis / Q000097:blood / Q000175:diagnosis / Q000382:microbiology; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature