Calretinin-immunoreactive systems in the cerebellum and cerebellum-related lateral-line medullary nuclei of an elasmobranch, Scyliorhinus canicula.
Calretinin immunohistochemistry was used to study the organization of some cerebellar structures and lateral line medullary nuclei of an elasmobranch, the lesser-spotted dogfish Scyliorhinus canicula. In the cerebellar molecular layer, stellate cells are strongly calretinin-immunoreactive (CR-ir). Perikarya and dendrites of Purkinje cells are contacted by numerous stellate cell small CR-ir boutons. Some Purkinje cell perikarya are contacted by CR-ir climbing fibers forming complex axo-somatic contacts. In the granular layer, numerous CR-ir mossy fibers exhibited large swellings. Notable differences in density and diameter of mossy fibers are observed between the auricles and cerebellar body. Thin beaded CR-ir fibers are also present in the granular layer of the body. The lateral line nuclei of the octavolateralis region are comprised of a molecular-like cerebellar crest that covers the dorsal (electroreceptive) and the medial octavolateralis nuclei (mechanoreceptive). The cerebellar crest exhibited numerous CR-ir stellate cells. In the dorsal octavolateralis nucleus, the presence of conspicuous CR-ir cells and neuropil closely associated to the region of primary fiber terminals distinguishes it clearly from the medial nucleus, revealing major differences between the electroreceptive and mechanoreceptive primary nuclei of elasmobranchs. Moreover, CR distribution in the dogfish cerebellum showed interesting differences with those reported in cerebella of other vertebrates, indicating a high variability of cerebellar CR expression in phylogeny.
Anadón
Ramón
R
Department of Cell Biology and Ecology, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. bfanadon@usc.es
Ferreiro-Galve
Susana
S
Sueiro
Catalina
C
Graña
Patricia
P
Carrera
Iván
I
Yáñez
Julián
J
Rodríguez-Moldes
Isabel
I
D000818:Animals; D064032:Calbindin 2; D002531:Cerebellum / Q000378:metabolism* / Q000648:ultrastructure; D004284:Dogfish / Q000033:anatomy & histology / Q000378:metabolism*; D007150:Immunohistochemistry; D053403:Lateral Line System / Q000502:physiology*; D008526:Medulla Oblongata / Q000378:metabolism* / Q000648:ultrastructure; D011689:Purkinje Cells / Q000378:metabolism / Q000648:ultrastructure; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis*
D064032:Calbindin 2; D064030:S100 Calcium Binding Protein G