title: Technical considerations in using DNA microarrays to define regulons.
abstract: Transcription is the major regulatory target of gene expression in bacteria, and is controlled by many regulatory proteins and RNAs. Microarrays are a powerful tool to study the regulation of transcription on a genomic scale. Here we describe the use of transcription profiling and ChIP-chip to study transcriptional regulation in bacteria. Transcription profiling determines the outcome of regulatory events whereas ChIP-chip identifies the protein-DNA interactions that determine these events. Together they can provide detailed information on transcriptional regulatory systems.
authors.affiliation: Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94143, USA. virgil.rhodius@ucsf.edu
authors.forename: Virgil A
authors.initials: VA
authors.lastname: Rhodius
authors.forename: Joseph T
authors.initials: JT
authors.lastname: Wade
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011485:Protein Binding; D018085:Regulon / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins