Self-assembling peptide nanofiber as potential substrates in islet transplantation.
Hypoxia and reoxygenation (H/R)-induced damage often happens soon after islets are transplantation. The process of islet isolation and purification causes the rapid onset of hypoxia. We sought to develop a functional scaffold to sustain the structure and function of islets as well as to recover some of the surface molecules damaged during isolation, seeking to improve islet transplantation outcomes. Self-assembling peptide nanofiber (SAPNF), a new type of substrate has been shown to be an excellent biological material for neuronal cell culture and tissue engineering in animals. In this study, we investigated the protective effect of SAPNF on damage to rat islets. Freshly prepared rat islets from male Sprague-Dawley rats were seeded in plates coated with (SAPNF-treated group) or without (control group) SAPNF. The islets were then divided into two groups culture under normoxia for 7 days versus exposure to hypoxia (< 1% O2) for 6 hours followed by reoxygenation for 24 hours. The results showed that SAPNF exhibited improving effects on viability and function of cultured islets, protecting the one from H/R-induced damage. In both groups, the stimulation index of SAPNF-treated groups were about two times the controls. SAPNF treatment decreased apoptotic rates of islet cells. These results suggested the usefulness of SAPNF to maintain the viability and function of rat pancreatic islets. SAPNF may be a potential scaffold for clinical islet transplantation.
Yuan
Y
Y
Key Lab of Transplant Engineering and Immunology, Ministry of Health, P R China.
Cong
C
C
Zhang
J
J
Wei
L
L
Li
S
S
Chen
Y
Y
Tan
W
W
Cheng
J
J
Li
Y
Y
Zhao
X
X
Lu
Y
Y
D000818:Animals; D017209:Apoptosis; D018929:Cell Culture Techniques / Q000379:methods; D015687:Cell Hypoxia; D002470:Cell Survival; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D010455:Peptides; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds
D007328:Insulin; D010455:Peptides; D005947:Glucose