title: An Antibody Against the SecA Membrane Protein of One Phytoplasma Reacts with Those of Phylogenetically Different Phytoplasmas.
abstract: ABSTRACT Antisera raised against phloem-limited phytoplasmas generally react only with the phytoplasma strain used to produce the antigen. There is a need for an antiserum that reacts with a variety of phytoplasmas. Here, we show that an antiserum raised against the SecA membrane protein of onion yellows phytoplasma, which belongs to the aster yellows 16S-group, detected eight phytoplasma strains from four distinct 16S-groups (aster yellows, western X, rice yellow dwarf, and elm yellows). In immunoblots, approximately 96-kDa SecA protein was detected in plants infected with each of the eight phytoplasmas. Immunohistochemical staining of thin sections prepared from infected plants was localized in phloem tissues. This antiserum should be useful in the detection and histopathological analysis of a wide range of phytoplasmas.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kakizawa
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
authors.forename: Shiho
authors.initials: S
authors.lastname: Suzuki
authors.forename: Minoru
authors.initials: M
authors.lastname: Tanaka
authors.forename: Hisashi
authors.initials: H
authors.lastname: Nishigawa
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Miyata
authors.forename: Kenro
authors.initials: K
authors.lastname: Oshima
authors.forename: Masashi
authors.initials: M
authors.lastname: Ugaki
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Hibi
authors.forename: Shigetou
authors.initials: S
authors.lastname: Namba