Analysis of hepatitis B virus X gene phylogeny, genetic variability and its impact on pathogenesis: implications in Eastern Indian HBV carriers.
HBx genetic variability was explored in the Eastern Indian population with low HCC incidence. DNase I sensitive HBV DNA was detected in 53% samples, which differed significantly between clinical groups (P<0.001). HBV genotypes A (Aa/A1), C (Cs/C1) and D (D1, D2, D3, D5) were detected in 37.5%, 18.7% and 43.7% samples respectively. Population specific signature HBx residues A(36), V(88), S(101) in Aa/A1 and residues P(41), Q(110) in D5 were detected. Mutations T(127), M(130) and I(131) were detected in 66.7%, 91% and 75% of genotype A, C and D5 samples respectively. Very low occurrence of HCC associated mutations (V(5)M/L, P(38)S, and H(94)Y) and absence of C-terminal deletions were observed. Our study shows that HBV genotype associated clinically important HBx variations may evolve and act distinctly in different geo-ethnic populations. Further studies on HBx functions from the perspective of genetic variability are essential for the better understanding of the clinical significance of HBV.
Datta
Sibnarayan
S
ICMR Virus Unit, Kolkata, ID and BG Hospital Campus, GB-4, 1st Floor (East Wing), 57, Dr. Suresh Chandra Banerjee Road, Pin-700010 (Beliaghata), Kolkata, West Bengal, India.
Banerjee
Arup
A
Chandra
Partha Kumar
PK
Biswas
Avik
A
Panigrahi
Rajesh
R
Mahapatra
Pradip Kumar
PK
Panda
Chinmoy Kumar
CK
Chakrabarti
Shekhar
S
Bhattacharya
Sujit Kumar
SK
Chakravarty
Runu
R
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D002353:Carrier State / Q000821:virology; D016384:Consensus Sequence; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation; D005838:Genotype; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins; D014774:Virulence / Q000235:genetics
D004279:DNA, Viral; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein