title: Potential chemoprevention effect of dietary fucoxanthin on urinary bladder cancer EJ-1 cell line.
abstract: Bladder cancer is a common but serious malignancy. It is widely accepted that chemoprevention may be an effective way to decrease the rate of recurrence and morbidity. We first determined antigrowth and apoptosis-induction activity of fucoxanthin from dietary Laminaria japonica against EJ-1 human bladder cancers. Fucoxanthin significantly reduced the cell viability in a dose- and time-dependent manner. The induction of apoptosis in EJ-1 cells was characterized by morphological changes, DNA ladder, and increased percentage of hypodiploid cells, activating caspase-3 activity. The ratio of apoptotic cells reached >93% after treatment for 72 h with 20 microM fucoxanthin. The findings obtained indicate that fucoxanthin may act as a chemopreventive and/or chemotherapeutic carotenoid in bladder cancer cells by modulating cell viability.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Zhang
authors.forename: Peijuan
authors.initials: P
authors.lastname: Zhang
authors.forename: Michito
authors.initials: M
authors.lastname: Hamada
authors.forename: Satoru
authors.initials: S
authors.lastname: Takahashi
authors.forename: Guoqing
authors.initials: G
authors.lastname: Xing
authors.forename: Jiqiang
authors.initials: J
authors.lastname: Liu
authors.forename: Norio
authors.initials: N
authors.lastname: Sugiura
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D018890:Chemoprevention; D004032:Diet; D005434:Flow Cytometry; D006801:Humans; D017382:Reactive Oxygen Species; D001749:Urinary Bladder Neoplasms / Q000517:prevention & control*; D024341:Xanthophylls / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; D024341:Xanthophylls; C025164:fucoxanthin