Effect of nutritional substrate on sulfolipids metabolic turnover in isolated renal tubules from rat.
Effects of a glycolytic (glucose) and a gluconeogenic renal nutritional substrate (glutamine) on metabolic turnover of sulfolipids, determined as [(35)S]sulfate incorporation, were compared in renal tubules prepared from well-fed rats. The results showed that the effects of glucose and glutamine, at nearly physiological serum concentration, are quite contrary to each other. Glucose increased the turnover rates of relatively long chain ganglio-series sulfoglycolipids (Gg(3)Cer II(3)-sulfate and Gg(4)Cer II(3),IV(3)-bis-sulfate) (1.7 to 2.4-fold), but not of cholesterol 3-sulfate (0.9-fold). In contrast, glutamine accelerated the turnover rates of relatively short chain sulfoglycolipids (glucosyl sulfatide, galactosyl sulfatide and lactosyl sulfatide) (1.3 to 2.7-fold), as well as cholesterol 3-sulfate (2.4-fold). The possible mechanism which causes these marked differences is also discussed.
Nagai
Ken-ichi
K
Department of Biochemistry, Faculty of Medicine, Teikyo University, Tokyo, Japan. knagai@med.teikyo-u.ac.jp
Tadano-Aritomi
Keiko
K
Niimura
Yukio
Y
Ishizuka
Ineo
I
D000818:Animals; D002788:Cholesterol Esters / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006019:Glycolysis; D066298:In Vitro Techniques; D007684:Kidney Tubules / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013462:Sulfur Radioisotopes / Q000378:metabolism
D002788:Cholesterol Esters; D008055:Lipids; D013462:Sulfur Radioisotopes; C015518:sulfolipids; D005973:Glutamine; D005947:Glucose; C007045:cholesteryl sulfate