Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet.
Human mitochondrial methionine transfer RNA (hmtRNA(Met)(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in (hmtRNA(Met)(CAU)) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.
Lusic
Hrvoje
H
Department of Chemistry, Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695 and Dharmacon, 2650 Crescent Drive #100, Lafayette, CO 80026, USA.
Gustilo
Estella M
EM
Vendeix
Franck A P
FA
Kaiser
Rob
R
Delaney
Michael O
MO
Graham
William D
WD
Moye
Virginia A
VA
Cantara
William A
WA
Agris
Paul F
PF
Deiters
Alexander
A
D000926:Anticodon / Q000737:chemistry*; D001483:Base Sequence; D003062:Codon / Q000378:metabolism; D003562:Cytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012313:RNA / Q000737:chemistry*; D000077278:RNA, Mitochondrial; D012358:RNA, Transfer, Met / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
D000926:Anticodon; D003062:Codon; D000077278:RNA, Mitochondrial; D012358:RNA, Transfer, Met; C086073:5-formylcytidine; D003562:Cytidine; D012313:RNA