A culture filtrate of Phytophthora infestans primes defense reaction in potato cell suspensions.
Priming of defense reactions by an elicitor results in an enhanced ability of the plant to respond to subsequent pathogen challenges. We previously showed that application of lipopolysaccharides (LPS) to potato cell suspensions causes apoplastic acidification, but does not stimulate lipoxygenase (LOX) activity. Here, we tested the ability of various elicitors to prime and elicit defense reactions in potato cell suspensions. Adding 20 microg ml(1) LPS, laminarin, harpin N, or a concentrated culture filtrate (CCF) of Phytophthora infestans to cell cultures 18 h before a second elicitation with LPS did not alter the intensity of apoplastic acidification compared with a single LPS application. Conversely, high concentrations (200 or 400 microg ml(1)) of LPS, laminarin, and harpin N activated LOX in cells pretreated with 1 microg ml(1) CCF, but not in cells pretreated with LPS, laminarin, or harpin N. LOX response was maximal in pretreated cells of potato cv. Bintje when the second elicitation occurred 18 to 24 h after CCF application. These results showed that LOX activation is primed in potato cells by CCF, but not by LPS, harpin N, or laminarin. Finally, bioassays showed a slightly greater reduction of rot weight in half tubers treated with CCF followed by LPS before inoculation with Pectobacterium atrosepticum than in half tubers treated with either preparation alone, indicating a priming effect of CCF on both LOX induction and disease suppression.
Val
F
F
INRA, Agrocampus Rennes, UMR1099 BiO3P (Biology of Organisms and Populations applied to Plant Protection), F-35000 Rennes, France. florence.val@agrocampus-rennes.fr
Desender
S
S
Bernard
K
K
Potin
P
P
Hamelin
G
G
Andrivon
D
D
D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005936:Glucans; D007113:Immunity, Innate / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D055750:Phytophthora infestans / Q000254:growth & development / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D011134:Polysaccharides / Q000494:pharmacology; D011198:Solanum tuberosum / Q000166:cytology / Q000187:drug effects / Q000382:microbiology*
D017077:Culture Media, Conditioned; D005936:Glucans; D008070:Lipopolysaccharides; D011134:Polysaccharides; C008247:laminaran