title: [Construction and identification of small interfering RNA expression plasmid targeting Sox9 and the function to cell growth and apoptosis of human chondrosarcoma cells HTB94].
abstract: OBJECTIVE
  To construct small interfering (siRNA) Sox9 expression plasmid and transfer it into human chondrosarcoma cells HTB-94, and to check the mRNA and protein expression of Sox9 and cell growth and apoptosis of HTB-94 human chondrosarcoma cells.
  
  
  METHODS
  siRNA(Sox9) expression plasmid was designed and synthesized. And it was transferred into HTB-94 human chondrosarcoma cells. Then the expression of the mRNA and protein of Sox9, cell growth and apoptosis in transferred HTB-94 human chondrosarcoma cells were checked.
  
  
  RESULTS
  The recombinant plasmid was confirmed by enzyme digestion analysis and DNA sequencing. The expression of the mRNA and protein expression of Sox9 in transferred HTB-94 were significantly reduced. The cell growth of HTB-94 was inhibited, and the apoptosis of HTB-94 was remarkably increased.
  
  
  CONCLUSIONS
  siRNA (Sox9) expression plasmid could be transferred into HTB-94 human chondrosarcoma cells. And it can reduce the mRNA and protein expression of the HTB-94, inhibit the cell growth and cause the apoptosis of the tumor cells.
authors.affiliation: Xuzhou Medical College Hospital, Xuzhou 221002, China. qinhongmin07@yahoo.com.cn
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Qin
authors.forename: Hui-Feng
authors.initials: HF
authors.lastname: Han
authors.forename: Tie
authors.initials: T
authors.lastname: Xu
authors.forename: Guang-Zhao
authors.initials: GZ
authors.lastname: Sha
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Yi-Gen
authors.initials: YG
authors.lastname: Peng
authors.forename: Tian-Cheng
authors.initials: TC
authors.lastname: Ren
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D002813:Chondrosarcoma / Q000378:metabolism / Q000473:pathology*; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D055758:SOX9 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D055758:SOX9 Transcription Factor