title: Prediction of lymph node metastasis by gene expression profiling in patients with primary resected lung cancer.
abstract: While lymph node metastasis is a major factor associated with poor prognosis in cancer, little is known of its molecular mechanisms. The aim of this study was to identify genes differentially expressed between non-cancerous and cancerous lung tissues, and to investigate the gene expression profiles of 41 primary lung adenocarcinomas to select sets of gene predictors for lymph node metastasis of lung cancer. Gene expression profiles were obtained using oligonucleotide microarrays, and predictor sets constructed by evaluating the statistical significance of expression levels of selected genes. Gene analysis revealed 15 predictor genes for lymph node metastasis of lung adenocarcinoma. Using the most suitable set of genes, it was possible to predict the lymph node metastasis of patients with lung cancer. The prediction scoring system yielded 71.4% accuracy for forecasting lymph node metastasis in 14 independent test cases. Survival was also significantly better in 18 cases that were pathologically LN negative and predicted to be LN negative according to molecular classification, compared with 23 cases that were pathologically LN positive or predicted to be LN positive according to molecular classification. Gene expression analysis combined with statistical analysis successfully distinguished lymph node metastasis. The findings of this study showed that pathological diagnosis combined with molecular classification clearly distinguished patients with good prognoses from patients with poor prognoses.
authors.affiliation: Department of Thoracic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan. moriya-ths@umin.ac.jp
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Moriya
authors.forename: Akira
authors.initials: A
authors.lastname: Iyoda
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kasai
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugimoto
authors.forename: Junya
authors.initials: J
authors.lastname: Hashida
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nimura
authors.forename: Masaki
authors.initials: M
authors.lastname: Kato
authors.forename: Masaki
authors.initials: M
authors.lastname: Takiguchi
authors.forename: Takehiko
authors.initials: T
authors.lastname: Fujisawa
authors.forename: Naohiko
authors.initials: N
authors.lastname: Seki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yoshino
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000556:secondary / Q000601:surgery; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D011446:Prospective Studies; D012189:Retrospective Studies; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor