BPH gene expression profile associated to prostate gland volume.
The aim of the current study was to analyze gene expression profiles in benign prostatic hyperplasia and to compare them with phenotypic properties. Thirty-seven specimens of benign prostatic hyperplasia were obtained from symptomatic patients undergoing surgery. RNA was extracted and hybridized to Affymetrix Chips containing 54,000 gene expression probes. Gene expression profiles were analyzed using cluster, TreeView, and significance analysis of microarrays softwares. In an initial unsupervised analysis, our 37 samples clustered hierarchically in 2 groups of 18 and 19 samples, respectively. Five clinical parameters were statistically different between the 2 groups: in group 1 compared with group 2, patients had larger prostate glands, had higher prostate specific antigen levels, were more likely to be treated by alpha blockers, to be operated by prostatectomy, and to have major irritative symptoms. The sole independent parameter associated with this dichotome clustering, however, was the prostate gland volume. Therefore, the role of prostate volume was explored in a supervised analysis. Gene expression of prostate glands <60 mL and >60 mL were compared using significance analysis of microarrays and 227 genes were found differentially expressed between the 2 groups (>2 change and false discovery rate of <5%). Several specific pathways including growth factors genes, cell cycle genes, apoptose genes, inflammation genes, and androgen regulated genes, displayed major differences between small and large prostate glands.
Descazeaud
Aurelien
A
INSERM, Unité 841, IMRB, Faculté de Médecine, University Paris 12, France. aureliendescazeaud@gmail.com
Rubin
Mark A
MA
Hofer
Matthias
M
Setlur
Sunita
S
Nikolaief
Nathalie
N
Vacherot
Francis
F
Soyeux
Pascale
P
Kheuang
Laurence
L
Abbou
Claude C
CC
Allory
Yves
Y
de la Taille
Alexandre
A
D000368:Aged; D000369:Aged, 80 and over; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D009929:Organ Size; D011467:Prostate / Q000473:pathology* / Q000502:physiology; D017430:Prostate-Specific Antigen / Q000097:blood; D011470:Prostatic Hyperplasia / Q000473:pathology* / Q000503:physiopathology; D015854:Up-Regulation
D017430:Prostate-Specific Antigen