Leptin gene, leptin gene receptor polymorphisms and body weight in pregnant women with type 1 diabetes mellitus.
Leptin, as well as many other hormones, may play an important role in the pathogenesis of obesity. Several genetic variants of both leptin and its receptor genes may influence human body weight


OBJECTIVE
To investigate the role of leptin gene polymorphism promotion region (-2548G/A) and leptin gene receptor polymorphism (668 A/G) in regulation of body weight in the group of women with type 1 diabetes (PGDM-1).


METHODS
78 PGDM-1 first trimester pregnant women were qualified for the study group (SG). They were divided into normal and overweight subgroups, based on pre-pregnancy BMI. Control group (CG) consisted of first trimester healthy pregnant women with normal pre-pregnancy body weight Genetic variants of leptin gene and its receptor were analyzed with the help of PCR-RFLP assays. In the SG, the following metabolic parameters were estimated: MBG, HbA1c, insulin dose, LDL, HDL, T-CHOL, triglycerids, creatinine, creatinine clearance and blood pressure.


RESULTS
A tendency for the majority of homozygous A and G variants in LEP -2548 G/A and LEPR 668 A/G was found in overweight and obese patients, in comparison to normal-weight subjects. No specific differences in selected first trimester metabolic parameters were found in relation to patients' genotypes in the diabetic group.
Iciek
Rafał
R
Department of Obsterics and Women Diseases, Poznań University of Medical Sciences, Poland. ralfiho@02.pl
Wender-Ozegowska
Ewa
E
Seremak-Mrozikiewicz
Agnieszka
A
Drews
Krzysztof
K
Brazert
Jacek
J
Pietryga
Marek
M
D000328:Adult; D015992:Body Mass Index; D001835:Body Weight / Q000235:genetics*; D016022:Case-Control Studies; D004252:DNA Mutational Analysis / Q000379:methods; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005260:Female; D006801:Humans; D020738:Leptin / Q000235:genetics*; D009765:Obesity / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000235:genetics*; D054411:Receptors, Leptin / Q000235:genetics*
C515448:LEPR protein, human; D020738:Leptin; D054411:Receptors, Leptin