Novel cold-adapted alkaline lipase from an intertidal flat metagenome and proposal for a new family of bacterial lipases.
A new lipase, LipEH166, isolated from an intertidal flat metagenome, showed no amino acid similarity to any known lipolytic enzyme except in the consensus region. This suggested that LipEH166 and its homologues belong to a new family of lipolytic enzymes. Partial characterization indicated that LipEH166 is a novel cold-adapted alkaline lipase.
Kim
Eun-Young
EY
Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yuseong, Daejon 305-333, Republic of Korea.
Oh
Ki-Hoon
KH
Lee
Mi-Hwa
MH
Kang
Chul-Hyung
CH
Oh
Tae-Kwang
TK
Yoon
Jung-Hoon
JH
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014871:Water Microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008049:Lipase