title: The value of Tzanck smear test in diagnosis of erosive, vesicular, bullous, and pustular skin lesions.
abstract: BACKGROUND
  Tzanck smear is generally used for the diagnosis of the pemphigus group of autoimmune bullous diseases and mucocutaneous herpesvirus infections. There are only a few studies in the literature investigating its diagnostic value.
  
  
  OBJECTIVE
  We aimed to investigate Tzanck smear findings and to determine the diagnostic value of this test in moist (erosive, vesicular, bullous, and pustular) skin lesions. We also aimed to develop an algorithmic approach for the diagnosis of these types of skin lesions according to the Tzanck smear findings.
  
  
  METHODS
  Samples were stained with May-Grünwald-Giemsa and evaluated by the same dermatologist. In some patients, methylene blue and Gram staining or direct immunofluorescence examinations were additionally performed. In all of the study cases, after the evaluation of clinical and laboratory findings (including, when appropriate, potassium hydroxide examination; viral serology; bacterial and fungal cultures; histopathology; direct and indirect immunofluorescence; patch testing), the definite diagnosis was established. We also determined the sensitivity and the specificity of certain Tzanck smear findings.
  
  
  RESULTS
  Tzanck smear was performed in a total of 400 patients with moist skin lesions. The sensitivities of multinucleated giant cells and acantholytic cells in herpetic infections, dyskeratotic acantholytic cells and cocci in bullous impetigo, pseudohyphae in candidiasis, acantholytic cells in pemphigus and more than 10 tadpole cells (magnification x100) in spongiotic dermatitis were 84.7%, 92%, 100%, 100%, and 81.5%, respectively.
  
  
  CONCLUSIONS
  Because Tzanck smears were evaluated by the same dermatologist, no comment could be made regarding the interobserver reliability of this test and how the level of experience with this technique might affect the results. Also, the sensitivity and the specificity of Tzanck smear test findings for certain diseases could not be calculated because of an insufficient number of patients.
  
  
  CONCLUSIONS
  The Tzanck smear test is an inexpensive, useful, and an easy diagnostic tool for certain skin diseases.
authors.affiliation: Department of Dermatology, Başkent University Faculty of Medicine, Adana Hospital, Adana, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Durdu
authors.forename: Mete
authors.initials: M
authors.lastname: Baba
authors.forename: Deniz
authors.initials: D
authors.lastname: Seçkin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000465:Algorithms; D002648:Child; D002675:Child, Preschool; D003581:Cytodiagnosis / Q000379:methods*; D004801:Eosine Yellowish-(YS); D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008751:Methylene Blue; D008875:Middle Aged; D011446:Prospective Studies; D012871:Skin Diseases / Q000175:diagnosis*; D012872:Skin Diseases, Vesiculobullous / Q000175:diagnosis*
chemical_list: C043260:May-Grunwald Giemsa; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)