title: Mebudipine and dibudipine protect PC12 cells against oxygen-glucose deprivation and glutamate-induced cell death.
abstract: The protective effect of two new L-type calcium-channel blockers, mebudipine and dibudipine on neurotoxic effects induced by glutamate and oxygen-glucose deprivation (OGD) in PC12 cells was investigated. PC12 cells were intoxicated with two different methods. First, the cells were incubated with glutamate (10muM/L), glutamate and mebudipine (10muM/L), dibudipine (10muM/L) or nimodipine (10muM/L), on three different treatment schedules (concurrently, pre-3h and pre-24h). In the second method PC12 cells were exposed to in vitro oxygen-glucose deprivation for 30min and 60min alone or with the drugs in the same time schedules described above. Cellular viability was assessed by MTT assay. Glutamate-induced cell death and OGD-induced cell injury were attenuated significantly by mebudipine, dibudipine in comparison with nimodipine in all three different treatment schedules. Application of MK801 (10muM/L), an antagonist of NMDA glutamate receptors inhibited PC12 cell death in both methods. Our study suggests that mebudipine and dibudipine, like nimodipine, may have protective effects against glutamate and oxygen-glucose deprivation-induced neurotoxicity.
authors.affiliation: Razi Institute of Drug Research, Iran University of Medical Science, Tehran, Iran.
authors.forename: Nahid
authors.initials: N
authors.lastname: Rahbar-Roshandel
authors.forename: Lili
authors.initials: L
authors.lastname: Razavi
authors.forename: Bahareh
authors.initials: B
authors.lastname: Tavakoli-Far
authors.forename: Massoud
authors.initials: M
authors.lastname: Mahmoudian