A novel C-type lectin from the shrimp Litopenaeus vannamei possesses anti-white spot syndrome virus activity.
C-type lectins play key roles in pathogen recognition, innate immunity, and cell-cell interactions. Here, we report a new C-type lectin (C-type lectin 1) from the shrimp Litopenaeus vannamei (LvCTL1), which has activity against the white spot syndrome virus (WSSV). LvCTL1 is a 156-residue polypeptide containing a C-type carbohydrate recognition domain with an EPN (Glu(99)-Pro(100)-Asn(101)) motif that has a predicted ligand binding specificity for mannose. Reverse transcription-PCR analysis revealed that LvCTL1 mRNA was specifically expressed in the hepatopancreas of L. vannamei. Recombinant LvCTL1 (rLvCTL1) had hemagglutinating activity and ligand binding specificity for mannose and glucose. rLvCTL1 also had a strong affinity for WSSV and interacted with several envelope proteins of WSSV. Furthermore, we showed that the binding of rLvCTL1 to WSSV could protect shrimps from viral infection and prolong the survival of shrimps against WSSV infection. Our results suggest that LvCTL1 is a mannose-binding C-type lectin that binds to envelope proteins of WSSV to exert its antiviral activity. To our knowledge, this is the first report of a shrimp C-type lectin that has direct anti-WSSV activity.
Zhao
Zhi-Ying
ZY
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen (Zhongshan) University, Guangzhou 510275, People's Republic of China.
Yin
Zhi-Xin
ZX
Xu
Xiao-Peng
XP
Weng
Shao-Ping
SP
Rao
Xia-Yu
XY
Dai
Zong-Xian
ZX
Luo
Yong-Wen
YW
Yang
Gan
G
Li
Zong-Sheng
ZS
Guan
Hao-Ji
HJ
Li
Se-Dong
SD
Chan
Siu-Ming
SM
Yu
Xiao-Qiang
XQ
He
Jian-Guo
JG
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000820:Animal Diseases / Q000517:prevention & control*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D001665:Binding Sites; D020869:Gene Expression Profiling; D005947:Glucose / Q000378:metabolism; D043143:Hepatopancreas / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000821:virology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D016019:Survival Analysis; D046848:White spot syndrome virus 1 / Q000187:drug effects*
D000998:Antiviral Agents; D037181:Lectins, C-Type; D005947:Glucose; D008358:Mannose