title: The p53 codon 72 single nucleotide polymorphism lacks a significant effect on implantation rate in fresh in vitro fertilization cycles: an analysis of 1,056 patients.
abstract: OBJECTIVE
  To determine whether the p53 codon 72 single nucleotide polymorphism, a change of the amino acid arginine (Arg) to proline (Pro) resulting from a single nucleotide mutation of guanine (G) to cytosine (C), has a clinically significant effect on implantation rate in fresh IVF cycles.
  
  
  METHODS
  Prospective cohort analysis.
  
  
  METHODS
  University-affiliated private IVF center.
  
  
  METHODS
  One thousand fifty-six female patients undergoing fresh nondonor IVF cycles.
  
  
  METHODS
  Embryo implantation rate.
  
  
  RESULTS
  Of the 1,056 patients (2,600 total embryos transferred) undergoing their first IVF cycle, 289 had no implantation events and attempted a second cycle. Of the 289 patients in their second cycle, 72 had no implantation events and attempted a third cycle. The p53 codon 72 single nucleotide polymorphism frequencies in the first cycle (homozygous major allele Arg/Arg [G_G] = 45%, heterozygous allele Arg/Pro [G_C] = 44%, and homozygous minor allele Pro/Pro [C_C] = 11%) did not differ significantly across subsequent IVF cycles. There was no statistically significant difference in embryo implantation rate with respect to the single nucleotide polymorphism.
  
  
  CONCLUSIONS
  The p53 codon 72 single nucleotide polymorphism lacks a clinically significant effect on embryo implantation rate in patients undergoing fresh nondonor IVF cycles.
authors.affiliation: University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey.
authors.forename: George
authors.initials: G
authors.lastname: Patounakis
authors.affiliation: University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey; Reproductive Medicine Associates of New Jersey, Morristown, New Jersey.
authors.forename: Nathan
authors.initials: N
authors.lastname: Treff
authors.affiliation: Reproductive Medicine Associates of New Jersey, Morristown, New Jersey.
authors.forename: Xin
authors.initials: X
authors.lastname: Tao
authors.affiliation: Reproductive Medicine Associates of New Jersey, Morristown, New Jersey.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Lonczak
authors.affiliation: University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey; Reproductive Medicine Associates of New Jersey, Morristown, New Jersey.
authors.forename: Richard T
authors.initials: RT
authors.lastname: Scott
authors.affiliation: University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey; Reproductive Medicine Associates of New Jersey, Morristown, New Jersey. Electronic address: jfrattarelli@rmanj.com.
authors.forename: John L
authors.initials: JL
authors.lastname: Frattarelli
mesh_terms: D003062:Codon / Q000235:genetics; D015331:Cohort Studies; D010064:Embryo Implantation / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005787:Gene Frequency; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007247:Infertility, Female / Q000175:diagnosis / Q000235:genetics / Q000628:therapy; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D011247:Pregnancy; D018873:Pregnancy Rate
chemical_list: D003062:Codon