title: Pregnancy, indoleamine 2,3-dioxygenase (IDO) and chlamydial abortion: an unresolved paradox.
abstract: Chlamydophila abortus infects the placental trophoblast in sheep, humans and mice, causing cell damage and inflammation that culminates in abortion. Host control of C. abortus appears to be heavily dependant on interferon (IFN)-gamma production. IFN-gamma induces expression of the enzyme indoleamine 2,3-dioxygenase (IDO), resulting in the degradation of intracellular pools of tryptophan, thereby depriving the organism of this essential growth nutrient. The anti-chlamydial effects of IFN-gamma can be reversed by the addition of exogenous tryptophan. This finding is consistent with studies of the C. abortus genome sequence that have revealed that the organism lacks the capability to synthesise tryptophan from host cell substrates and is therefore dependant on host tryptophan. This raises an interesting paradox since the placental trophoblast in humans and mice is known to constitutively express IDO and degrade tryptophan, a phenomenon that has been linked to maternal immunological tolerance of the semi-allogeneic fetus. This paradox is discussed in the context of immune modulation during pregnancy, tryptophan biosynthesis by Chlamydiaceae and differences in placental structures between sheep, humans and mice.
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian EH26 0PZ, United Kingdom. gary.entrican@moredun.ac.uk
authors.forename: Gary
authors.initials: G
authors.lastname: Entrican
authors.forename: Sean
authors.initials: S
authors.lastname: Wattegedera
authors.forename: Mara
authors.initials: M
authors.lastname: Rocchi
authors.forename: Nicholas
authors.initials: N
authors.lastname: Wheelhouse
mesh_terms: D000022:Abortion, Spontaneous / Q000382:microbiology*; D000818:Animals; D023521:Chlamydophila Infections / Q000150:complications / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D011247:Pregnancy; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology; D014364:Tryptophan / Q000096:biosynthesis
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007371:Interferon-gamma; D014364:Tryptophan