iTRAQ analysis of Singapore grouper iridovirus infection in a grouper embryonic cell line.
We report, here, the first proteomics study of a grouper embryonic cell line (GEC) infected by Singapore grouper iridovirus (SGIV). The differential proteomes of GEC with and without viral infection were studied and quantified with iTRAQ labelling followed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Forty-nine viral proteins were recognized, of which 11 were identified for the first time. Moreover, 743 host proteins were revealed and classified into 218 unique protein groups. Fourteen host proteins were upregulated and five host proteins were downregulated upon viral infection. The iTRAQ analysis of SGIV infection in GEC provides an insight to viral and host gene products at the protein level. This should facilitate further study and the understanding of virus-host interactions, molecular mechanisms of viral infection and pathogenesis.
Chen
Li Ming
LM
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
Tran
Bich Ngoc
BN
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
Lin
Qingsong
Q
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
Lim
Teck Kwang
TK
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
Wang
Fan
F
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
Hew
Choy-Leong
CL
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D005393:Fish Diseases / Q000821:virology; D017781:Genes, Immediate-Early; D005814:Genes, Viral; D017994:Iridovirus / Q000235:genetics* / Q000472:pathogenicity; D007553:Isotope Labeling / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D010473:Perciformes / Q000196:embryology / Q000821:virology; D025821:Polyubiquitin / Q000737:chemistry / Q000235:genetics; D012846:Singapore; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014777:Virus Diseases / Q000821:virology
D014764:Viral Proteins; D025821:Polyubiquitin