Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli.
The yncE gene of Escherichia coli encodes a predicted periplasmic protein of unknown function. The gene is de-repressed under iron restriction through the action of the global iron regulator Fur. This suggests a role in iron acquisition, which is supported by the presence of the adjacent yncD gene encoding a potential TonB-dependent outer-membrane transporter. Here, the preliminary crystallographic structure of YncE is reported, revealing that it consists of a seven-bladed beta-propeller which resembles the corresponding domain of the ;surface-layer protein' of Methanosarcina mazei. A full structure determination is under way in order to provide insight into the function of this protein.
Baba-Dikwa
Aisha
A
School of Biological Sciences, University of Reading, Whiteknights Campus, Reading, Berkshire RG6 6AS, England.
Thompson
Darren
D
Spencer
Nick J
NJ
Andrews
Simon C
SC
Watson
Kimberly A
KA
D003460:Crystallization; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D007501:Iron; D008958:Models, Molecular; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D014961:X-Ray Diffraction
D017931:DNA Primers; D029968:Escherichia coli Proteins; C533370:YncE protein, E coli; D007501:Iron