title: Effect of profound hypothermia on genomics of hippocampus following complete cerebral ischemia in rats.
abstract: OBJECTIVE
  To determine differential gene expression of hippocampus in rats following complete cerebral ischemia with treatment of profound hypothermia compared to normothermia.
  
  
  METHODS
  Six rats got 5 minutes of complete cerebral ischemia with circulatory arrest and randomly divided into two groups: normothermia ischemia group (37 +/- 0.3 degrees C, n = 3) and profound hypothermia ischemia group (18 +/- 0.5 degrees C, n = 3). Affymetrix U34A rat arrays were applied to detect the difference of gene expression profile in hippocampus between the two groups.
  
  
  RESULTS
  Expression profiles of a total of 75 transcripts in the profound hypothermia ischemia group were statistically different from those of the normothermia ischemia group, and 33 of them were significantly up-regulated and other 42 were significantly down-regulated (p < 0.07).
  
  
  CONCLUSIONS
  Compared with normothermia, profound hypothermia had a significant effect on the gene expression profiles following complete cerebral ischemia, which may be involved in the mechanisms of cerebral protection by profound hypothermia.
authors.affiliation: Department of Neurosurgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
authors.forename: Hua-Ping
authors.initials: HP
authors.lastname: Qin
authors.forename: Guang-Hai
authors.initials: GH
authors.lastname: Mei
authors.forename: Liang
authors.initials: L
authors.lastname: Wei
authors.forename: Ji-Yao
authors.initials: JY
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000473:pathology* / Q000628:therapy*; D030541:Databases, Genetic / Q000706:statistics & numerical data; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D023281:Genomics / Q000379:methods*; D006624:Hippocampus / Q000378:metabolism*; D007036:Hypothermia, Induced / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011897:Random Allocation; D051381:Rats