Dimethyl sulfoxide perfusion in caprine ovarian tissue and its relationship with follicular viability after cryopreservation.
Ovarian cortical fragments (3 x 3 x 1 mm) were exposed to dimethyl sulfoxide (DMSO) in different concentrations for further analysis of cryoprotectant perfusion by applying high-performance liquid chromatography (HPLC) and conventional cryopreservation. This simple perfusion test can predict the efficiency of the cryopreservation procedure.
Luz
Valesca B
VB
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles, University of Ceará, Fortaleza, Brazil.
Santos
Regiane R
RR
Pinto
Leonardo C
LC
Soares
Alison A X
AA
Celestino
Juliana J H
JJ
Mafezoli
Jair
J
Campello
Cláudio C
CC
Figueiredo
José R
JR
Rodrigues
Ana P R
AP
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006041:Goats; D023421:Models, Animal; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D010053:Ovary / Q000187:drug effects* / Q000502:physiology; D010477:Perfusion / Q000379:methods; D014022:Tissue Survival / Q000187:drug effects* / Q000502:physiology
D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide