Characterization of infectious hepatitis C virus from liver-derived cell lines.
The efficient production of infectious HCV from the JFH-1 strain is restricted to the Huh7 cell line and its derivatives. However, the factors involved in this restriction are unknown. In this study, we examined the production of infectious HCV from other liver-derived cell lines, and characterized the produced viruses. Clones of the Huh7, HepG2, and IMY-N9, harboring the JFH-1 full-genomic replicon, were obtained. The supernatant of each cell clone exhibited infectivity for naïve Huh7. Each infectious supernatant was then characterized by sucrose density gradient. For all of the cell lines, the main peak of the HCV-core protein and RNA exhibited at approximately 1.15g/mL of buoyant density. However, the supernatant from the IMY-N9 differed from that of Huh7 in the ratio of core:RNA at 1.15g/mL and significant peaks were also observed at lower density. The virus particles produced from the different cell lines may have different characteristics.
Akazawa
Daisuke
D
Pharmaceutical Research Laboratories, Toray Industries, Inc., Kanagawa, Japan.
Date
Tomoko
T
Morikawa
Kenichi
K
Murayama
Asako
A
Omi
Noriaki
N
Takahashi
Hitoshi
H
Nakamura
Noriko
N
Ishii
Koji
K
Suzuki
Tetsuro
T
Mizokami
Masashi
M
Mochizuki
Hidenori
H
Wakita
Takaji
T
D002460:Cell Line; D016174:Hepacivirus / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry; D014779:Virus Replication
D012367:RNA, Viral