Validation of a method for the determination of sterols and triterpenes in the aerial part of Justicia anselliana (Nees) T. Anders by capillary gas chromatography.
An accurate and sensitive method, combining soxhlet extraction, solid phase-extraction and capillary gas chromatography is described for the quantitative determination of one triterpene (lupeol) and three sterols (stigmasterol, campesterol and beta-sitosterol) and the detection of another triterpene (alpha-amyrin) from the aerial part of Justicia anselliana. This is the first method allowing the quantification of sterols and triterpenes in this plant. It has been fully validated in order to be able to compare the sterol and triterpene composition of different samples of J. anselliana and therefore help to explain the allelopathic activity due to these compounds. This method showed that the aerial part of J. anselliana contained (292+/-2)mg/kg of lupeol, (206+/-1)mg/kg of stigmasterol, (266+/-2)mg/kg of campesterol and (184+/-9)mg/kg of beta-sitosterol.
Kpoviéssi
Dossou Sika Salomé
DS
Pharmacognosy and Essential Oils Laboratory, Faculty of Health Science (FSS), Faculty of Sciences and Technics (FAST), University of d'Abomey-Calavi (UAC), 01 BP 188 Cotonou, Benin. 30-CHAM, Av Mounier 72, B-1200 Bruxelles, Belgium.
Gbaguidi
Fernand
F
Gbénou
Joachim
J
Accrombessi
Georges
G
Moudachirou
Mansourou
M
Rozet
Eric
E
Hubert
Philippe
P
Quetin-Leclercq
Joëlle
J
D027901:Acanthaceae / Q000737:chemistry*; D002138:Calibration; D002784:Cholesterol / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes; D010840:Phytosterols / Q000032:analysis / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012855:Sitosterols / Q000032:analysis / Q000737:chemistry; D052616:Solid Phase Extraction; D013261:Sterols / Q000032:analysis* / Q000737:chemistry; D013265:Stigmasterol / Q000032:analysis / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry; D000094042:Lupanes
D002784:Cholesterol; D053978:Pentacyclic Triterpenes; D010840:Phytosterols; D012855:Sitosterols; D013261:Sterols; D013265:Stigmasterol; D014315:Triterpenes; C021273:campesterol; C025473:gamma-sitosterol; C010480:lupeol; D000094042:Lupanes