title: Nuclear envelope formation in vitro: a sea urchin egg cell-free system.
abstract: The formation of the nuclear envelope (NE) typically occurs once during every mitotic cycle in somatic cells, and also around the sperm nucleus following fertilization. Much of our understanding of NE assembly has been derived from systems modeling the latter event in vitro. In these systems, demembranated sperm nuclei are combined with fertilized egg cytoplasmic extracts and an ATP-regenerating system and in a multistep process they form the functional double bilayer of the NE. Using a system that we developed from sea urchin gametes, we have demonstrated that NE assembly is regulated by membrane vesicles in a spatial and temporal fashion, emphasizing the roles of phosphoinositides, particularly phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)), diacylglycerols (DAG), and lipid-modifying enzymes in NE assembly.
authors.affiliation: Cell Biophysics Laboratory, Cancer Research UK (CR-UK), London Research Institute (LRI), London, UK.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Byrne
authors.forename: Vanessa
authors.initials: V
authors.lastname: Zhendre
authors.forename: Banafshé
authors.initials: B
authors.lastname: Larijani
authors.forename: Dominic L
authors.initials: DL
authors.lastname: Poccia
mesh_terms: D000818:Animals; D002474:Cell-Free System / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009685:Nuclear Envelope / Q000378:metabolism* / Q000502:physiology; D010063:Ovum / Q000378:metabolism* / Q000502:physiology; D010713:Phosphatidylcholines / Q000378:metabolism; D012617:Sea Urchins / Q000378:metabolism* / Q000502:physiology; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D010713:Phosphatidylcholines; D002784:Cholesterol; D010738:Type C Phospholipases