A novel solid phase- and chemical crosslinking-based technology for determining protein localization in biological supramolecules.
A solid phase- and chemical crosslinking-based technology was developed for determining the depths at which various protein constituents reside in a supramolecule. The usefulness of this technology was verified by trials using a synthetic three-protein complex on glass coverslips. This technology was further applied to investigate the localization of seven major protein components in the postsynaptic density, a landmark supramolecule of the excitatory synapses in mammalian brains. The technology reported here will supplement the already powerful proteomic methodologies in studying the structure/function relationships of supramolecules.
Horng
Wei-Cheng
WC
Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan, ROC.
Yen
Yuan-Hun
YH
Chang
Yen-Chung
YC
D001386:Azides; D003432:Cross-Linking Reagents; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000737:chemistry; D011437:Propylamines; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D013388:Succinimides
D001386:Azides; D003432:Cross-Linking Reagents; D009419:Nerve Tissue Proteins; D011437:Propylamines; D011506:Proteins; D013388:Succinimides; C033652:postsynaptic density proteins; C074882:sulfosuccinimidyl-2-(7-azido-4-methylcoumarin-3-acetamido)ethyl-1,3'-dithiopropionate