title: Penicillium griseofulvum F1959, high-production strain of pyripyropene a, specific inhibitor of acyl-CoA: cholesterol acyltransferase 2.
abstract: Acyl-coenzyme A: cholesterol acyltransferase (ACAT) catalyzes cholesterol esterification and plays an important role in the intestinal absorption of cholesterol, hepatic production of lipoproteins, and accumulation of cholesteryl ester within cells. During the course of screening to find ACAT inhibitors from microbial sources, the present authors isolated pyripyropene A from Penicillium griseofulvum F1959. Pyripyropene A, an ACAT2-specific inhibitor, has already been produced from Aspergillus fumigatus. Yet, Aspergillus fumigatus is a pathogen and only produces a limited amount of pyripyropene A, making the isolation of pyripyropene A troublesome. In contrast, Penicillium griseofulvum F1959 was found to produce approximately 28 times more pyripyropene A than Aspergillus fumigatus, plus this report also describes the ideal conditions for the production of pyripyropene A by Penicillium griseofulvum F1959 and its subsequent purification.
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
authors.forename: Ji Na
authors.initials: JN
authors.lastname: Choi
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Bae
authors.forename: Koanhoi
authors.initials: K
authors.lastname: Kim
authors.forename: Young Kook
authors.initials: YK
authors.lastname: Kim
mesh_terms: D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010407:Penicillium / Q000737:chemistry / Q000378:metabolism*; D011725:Pyridines / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D011725:Pyridines; D012717:Sesquiterpenes; C082792:pyripyropene A; D002785:Sterol O-Acyltransferase