[The protein oxidative damage level and lifespan modulation by xenobiotics in Drosophila melanogaster].
The effect of xenobiotics on protein oxidative damage and lifespan of Drosophila melanogaster Meig., line Oregon-R, was studied. Addition of the uncoupler 2,4-dinitrophenol (DNP) to the nutritional mixture results in an induction of synthesis of heat shock proteins and increase of lifespan of insects, whereas sodium nitroprusside (SNP) has negative effect on flies viability connected, probably, with activation of processes of proteins oxidative damage. It is shown that DNP essentially corrects the SNP negative action on insects' survival rates and this "normalizing" action is revealed both at a level of sensitivity of flies to exogenic stresses and protein carbonils level and at a level of insects lifespan as a whole. It is supposed that DNP protects from SNP negative action on flies viability by reduction of intensity of free radicals production and/or induction of heat shock proteins synthesis. Consequence of that is reduction of oxidative proteins damage degree and increase of survival rate (life span) of flies.
Padalko
V I
VI
Leonova
I S
IS
Kozlova
E V
EV
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000378:metabolism*; D018869:Heat-Shock Response / Q000187:drug effects; D008136:Longevity / Q000187:drug effects*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D015262:Xenobiotics / Q000494:pharmacology*
D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; D015262:Xenobiotics; D009599:Nitroprusside; D000426:Alcohol Dehydrogenase; D019297:2,4-Dinitrophenol