Cytokine profile associated with chronic and acute human schistosomiasis mansoni.
The production and regulation of interleukin (IL) IL-13, IL-4 and interferon-gamma (IFN-gamma) was evaluated in 43 schistosomiasis patients with different clinical forms. Whole-blood cultures cytokine production in response to soluble egg antigen (SEA), soluble worm adult preparation (SWAP), mitogens, neutralizing antibodies or recombinant IL-13 were measured by ELISA. After SWAP stimulation, chronic patients, particularly hepatointestinals, produced higher levels of IL-4 in comparison with acute patients, suggesting the presence of a type 2 cytokine profile in these patients. Following SEA and SWAP stimulation, hepatosplenic (HS) patients showed increased levels of IFN-gamma when compared with acute patients, indicating that HS disease in humans is associated with a type 1 cytokine response. The mechanisms of immune regulation are apparently different between the clinical stages of the disease, some of which are antigen-specific.
Morais
Clarice Neuenschwander Lins de
CN
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE, Brasil. clarice@cpqam.fiocruz.br
Souza
Joelma Rodrigues de
JR
Melo
Wlademir Gomes
WG
Aroucha
Marcílio L
ML
Miranda
Paulo
P
Domingues
Ana Lúcia Coutinho
AL
Abath
Frederico Guilherme Coutinho
FG
Montenegro
Silvia Maria Lucena
SM
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007377:Interleukin-3 / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
D007377:Interleukin-3; D015847:Interleukin-4; D007371:Interferon-gamma