[Assessment of the young rat model of visceral hypersensitivity by measuring electrical discharge of external oblique].
OBJECTIVE
To study the value of measuring electrical discharge of external oblique in assessment of young rat model of visceral hypersensitivity.


METHODS
Eight-day-old neonatal Sprague-Dawley rats were randomly assigned to two groups: an experimental group and a control group (n=16 each). Rats in the experimental group were subjected to mechanical colorectal irritation daily for 7 consecutive days, while the rats in the control group did not received colorectal irritation treatment. On the 6th week of their lives, the spike amplitude of external oblique were measured to evaluate the bowel sensitivity.


RESULTS
When the colorectal distention (CRD) pressure was 30 and 45 mmHg, the 95% confidence interval of the spike amplitude in the experimental group was significantly higher than that in the control group (P<0.01). When the CRD pressure were 60 and 75 mmHg, the 95% confidence interval of the spike amplitude in female rats was significantly higher than that in males (P<0.05).


CONCLUSIONS
The electrical discharge of external oblique confirmed that chronic colorectal irritation in neonatal rats can result in a chronic visceral hypersensitivity in the juvenile stage, with gender differences. Electrophysiological assessment is a quantitative test, and can objectively reflect visceral sensibility of pain.
Yang
Yan-Zhen
YZ
Department of Pediatrics, First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China.
Wu
Bin
B
Zhang
Rui
R
Zhuo
Ling
L
Chen
Jing-Fang
JF
Lin
Guo-Wei
GW
Lin
Chun
C
D000818:Animals; D003106:Colon / Q000503:physiopathology*; D004195:Disease Models, Animal; D005260:Female; D043183:Irritable Bowel Syndrome / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012007:Rectum / Q000503:physiopathology*; D012018:Reflex / Q000502:physiology