title: Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli.
abstract: The yncE gene of Escherichia coli encodes a predicted periplasmic protein of unknown function. The gene is de-repressed under iron restriction through the action of the global iron regulator Fur. This suggests a role in iron acquisition, which is supported by the presence of the adjacent yncD gene encoding a potential TonB-dependent outer-membrane transporter. Here, the preliminary crystallographic structure of YncE is reported, revealing that it consists of a seven-bladed beta-propeller which resembles the corresponding domain of the ;surface-layer protein' of Methanosarcina mazei. A full structure determination is under way in order to provide insight into the function of this protein.
authors.affiliation: School of Biological Sciences, University of Reading, Whiteknights Campus, Reading, Berkshire RG6 6AS, England.
authors.forename: Aisha
authors.initials: A
authors.lastname: Baba-Dikwa
authors.forename: Darren
authors.initials: D
authors.lastname: Thompson
authors.forename: Nick J
authors.initials: NJ
authors.lastname: Spencer
authors.forename: Simon C
authors.initials: SC
authors.lastname: Andrews
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Watson
mesh_terms: D003460:Crystallization; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D007501:Iron; D008958:Models, Molecular; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D017931:DNA Primers; D029968:Escherichia coli Proteins; C533370:YncE protein, E coli; D007501:Iron