[Stimulating autophagosome formation and inducing mixed cell death in HeLa cells by troglitazone].
OBJECTIVE
To study the effect of troglitazone on autophagy and programmed cell death of HeLa cells.


METHODS
Fluorescence microscopy, electron microscopy, western-blotting and flow cytometry were used.


RESULTS
Troglitazone increased the autophagosome formation of HeLa cell as observed by fluorescence and electron microscopy. Immunoblotting further confirmed cell morphology observation upon the reagent stimulating by detecting an enhanced expression of LC3, an autophagic related gene. Besides apoptosis, troglitazone triggered a necrotic cell death in flow cytometry analysis.


CONCLUSIONS
Troglitazone induced mixed type of cell death in HeLa cells.
Zhang
Wei
W
Institute of Microgbiology, Chinese Academy of Sciences, Beijing 100101, China. zhangwei_zifeiyu@hotmail.com
Meng
Xiang'ai
X
Yan
Jinguo
J
Jiang
Xuejun
X
D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D015153:Blotting, Western; D002839:Chromans / Q000494:pharmacology*; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009336:Necrosis; D010588:Phagosomes / Q000187:drug effects* / Q000378:metabolism*; D045162:Thiazolidinediones / Q000494:pharmacology*; D000077288:Troglitazone
D002839:Chromans; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D045162:Thiazolidinediones; D000077288:Troglitazone