Molecular epidemiology of dengue virus serotype 2 in the Taiwan 2002 outbreak with envelope gene and nonstructural protein 1 gene analysis.
The genetic relationships among dengue virus serotype 2 (DEN-2) isolates from the Taiwan 2002 epidemic were studied by sequence analysis of the envelope (E) and nonstructural protein 1 (NS1) genes. A 0-0.4% divergence among 10 isolates revealed an epidemic strain in the outbreak. Phylogenetic study demonstrated that the 2002 Taiwan isolates were of the Cosmopolitan genotype, which is different from the Asian 1 and Asian 2 genotypes of Taiwan DEN-2 isolates from 1981 to 1998 and the American/Asian genotype of 2005 Taiwan isolates. Although grouping results from both E and NS1 gene sequence analyses were the same, the usage of the NS1 gene as a sequence analysis target has not been validated for the lower bootstrap support values of branches in the phylogenetic tree. Our result showing the same genotype changes in Taiwan and Philippines isolates suggests strain transfer of DEN-2 to nearby countries resulting in the same trend of genotype change.
Tung
Yi-Ching
YC
Department of Public Health, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Lin
Kuei-Hsiang
KH
Chiang
Hung-Che
HC
Ke
Liang-Yin
LY
Chen
Yen-Hsu
YH
Ke
Guan-Ming
GM
Chen
Tun-Chieh
TC
Chou
Lee-Chiu
LC
Lu
Po-Liang
PL
D000330:Aedes; D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004196:Disease Outbreaks; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D019595:Severe Dengue / Q000453:epidemiology* / Q000821:virology; D013624:Taiwan / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism
D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins