The phospholipase A inhibitor, aristolochic acid, disrupts cortical microtubule arrays and root growth in Arabidopsis.
The role of phospholipase A(2) in Arabidopsis root growth and microtubule organisation was investigated using a specific inhibitor, aristolochic acid. At 0.5-1.5 microm concentrations, this inhibitor reduced root elongation and caused radial swelling of the root tip. The normally transverse cortical microtubules in root tip cells became progressively more disorganised with increasing concentrations of the inhibitor. Microtubule disorganisation also occurred in leaf epidermal cells of Allium porrum. We propose that phospholipase A(2) is involved in microtubule organisation and anisotropic growth in a manner similar to that reported previously for phospholipase D, thus broadening the significance of phospholipid signalling in microtubule organisation in plants.
Gardiner
J
J
School of Biological Sciences, University of Sydney, Sydney, NSW, Australia.
Andreeva
Z
Z
Barton
D
D
Ritchie
A
A
Overall
R
R
Marc
J
J
D000490:Allium / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000378:metabolism; D034341:Aristolochic Acids / Q000494:pharmacology*; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology; D064801:Phospholipase A2 Inhibitors; D019441:Plant Epidermis / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*
D029681:Arabidopsis Proteins; D034341:Aristolochic Acids; D064801:Phospholipase A2 Inhibitors; C000228:aristolochic acid I