title: Searching biomarker candidates in serum using multidimensional native chromatography. II Method evaluation with Alport syndrome and severe inflammation.
abstract: Biomarker search using multidimensional native liquid fractionation of serum in microplates was evaluated. From different donors, homologous sample fractions with UV absorbance depending on state of illness were selected, and their constituents were identified and quantitated by MS. Analysis of sera of patients with Alport syndrome and severe inflammation proved the reliability of the method by confirming characteristic alterations. Moreover, 23 new marker candidates were detected for Alport syndrome, some of them being involved in matrix degradation and repair, and 33 new candidates for severe inflammation, among them alpha1B-glycoprotein cysteine-rich secretory protein and an apparently low molecular-weight albumin variant.
authors.affiliation: Institute of Biochemistry, Friedrich Schiller University, Medical Faculty, Jena, Germany.
authors.forename: Adrienne
authors.initials: A
authors.lastname: Baum
authors.forename: Michael
authors.initials: M
authors.lastname: Pohl
authors.forename: Stefan
authors.initials: S
authors.lastname: Kreusch
authors.forename: Gerhard A
authors.initials: GA
authors.lastname: Cumme
authors.forename: Günter
authors.initials: G
authors.lastname: Ditze
authors.forename: Joachim
authors.initials: J
authors.lastname: Misselwitz
authors.forename: Michael
authors.initials: M
authors.lastname: Kiehntopf
authors.forename: Lene
authors.initials: L
authors.lastname: Udby
authors.forename: Andreas
authors.initials: A
authors.lastname: Meier-Hellmann
authors.forename: Heidrun
authors.initials: H
authors.lastname: Rhode
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000097:blood*; D005591:Chemical Fractionation / Q000379:methods; D002648:Child; D005260:Female; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D008297:Male; D009394:Nephritis, Hereditary / Q000097:blood*; D018805:Sepsis / Q000097:blood*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: C048566:A1BG protein, human; D015415:Biomarkers; D006023:Glycoproteins; D007136:Immunoglobulins