title: NP-induced biophysical and biochemical alterations of rat testicular Sertoli cell membranes related to disturbed intracellular Ca(2+) homeostasis.
abstract: Nonylphenol (NP) is a representative endocrine disruptor that has an adverse effect on male reproduction and posses direct hazard to Sertoli cells, but the mechanism remains incompletely elucidated. In the present study, based on the structural comparability and high affinity between NP and membrane phospholipid molecules, we tested the hypothesis that entrance of NP into Sertoli cells would alter membrane biophysical characteristics and biochemical functions. First, we used gas chromatography-mass spectrometry (GC-MS) to investigate the distribution and pharmacokinetics of NP in Sertoli cells with the result revealing that NP could penetrate plasma membrane of Sertoli cells. Meanwhile, Sertoli cells treated with NP exhibited abnormal membrane potential; that is an early depolarization following short treatment and hyperpolarization after longer treatment with the highest concentration of NP. Studies on the membrane dynamics indicated that the NP exposure rendered increased membrane fluidity and decreased microviscosity and molecular order. The result of lactate dehydrogenase (LDH) leakage assay demonstrated that NP increased membrane permeability in time-dose-dependent manners. Atomic force microscopic (AFM) imaging was applied to examine the membrane topography, and the images showed that NP treatment caused disturbance of membrane topography. The activities of plasma membrane Ca(2+)-ATPase, Ca(2+)-Mg(2+)-ATPase and Na(+)-K(+)-ATPase were also changed following NP exposure. However, FSH receptor as an important membrane protein was not significantly altered. All the above changes led to the disturbed intracellular Ca(2+) homeostasis which was an important signal triggering apoptosis. Hence, cellular membranes represented a plausible target for NP-induced cytotoxicity.
authors.affiliation: Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing 210093, P R China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gong
authors.forename: Xuping
authors.initials: X
authors.lastname: Pan
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Huang
authors.forename: Zishen
authors.initials: Z
authors.lastname: Gao
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Han
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity; D008401:Gas Chromatography-Mass Spectrometry; D006706:Homeostasis / Q000187:drug effects*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D010636:Phenols / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011962:Receptors, FSH / Q000378:metabolism; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013501:Surface-Active Agents / Q000633:toxicity
chemical_list: D052244:Endocrine Disruptors; D010636:Phenols; D011962:Receptors, FSH; D013501:Surface-Active Agents; C025256:nonylphenol; D000251:Adenosine Triphosphatases; D002118:Calcium