[Initial screening of binding-peptide of the cell surface marker CD133 of cancer stem cells].
OBJECTIVE
To select the binding-peptide of the cell surface marker CD133 of cancer stem cells from phage peptide library, and to find a new tool for research on stem cells, tumor therapy and anti-metastasis of cancer.


METHODS
Biotined mouse CD133 extracellular fraction was used as a target to screen phage 7-peptide library by the high affinity of streptavidin and biotin, and the clones were identified by sandwich ELISA and competitive experiment. Single strand DNA was extracted from these positive clones and was analyzed by single-strand dideoxy-sequencing.


RESULTS
After three turn solution panning, five peptides with high affinity shared the same amino acid sequence: APSPMIW and three identical peptides with high affinity shared the same amino acid sequence: LQNAPRS.


CONCLUSIONS
The peptides that bind with mouse CD133 extracellular fraction with high affinity and specificity were first screened from the phage peptide library for the first time, which initially indicates that the feasibility of screening from phage peptide library with small molecule polypeptide biotined as a target.
Sun
Jin-min
JM
Department of Pathology,Southern Medical University, Key Laboratory of Molecule Tumor Pathology of Guangdong Province, Guangzhou 510515, China.
Zhang
Chao
C
Li
Xue-nong
XN
D000071916:AC133 Antigen; D000818:Animals; D000906:Antibodies / Q000276:immunology; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014408:Biomarkers, Tumor; D003001:Cloning, Molecular; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D017422:Sequence Analysis, DNA
D000071916:AC133 Antigen; D000906:Antibodies; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; C000606023:PROM1 protein, human; D019151:Peptide Library; D010455:Peptides; C000606024:Prom1 protein, mouse