The effects of BADGE and caffeine on the time-course response of adiponectin and lipid oxidative enzymes in high fat diet-fed C57BL/6J mice: correlation with reduced adiposity and steatosis.
Adiponectin, which is expressed exclusively in adipose tissue, has been shown to increase fatty acid oxidation via activation of AMP-activated kinase (AMPK) and phosphorylation of acetyl CoA carboxylase (ACC). ACC phosphorylation and carnitine palmitoyl-transferase-1 (CPT1) activity have been shown to be rate controlling factors in fatty acid oxidation. In high fat diet (HFD)-induced obese mice, we analyzed the time-course of changes in the expression of adiponectin and lipid oxidative enzymes induced by treatment with bisphenol A diglycidyl ether (BADGE) or caffeine for 8 weeks, and investigated whether the changes of adiponectin and lipid oxidative enzymes expression correlated with reduced adiposity or steatosis after 8 weeks of treatment. After 8 weeks of treatment, BADGE and caffeine had reduced body weight and epididymal adipose tissue weight in mice fed HFD, and markedly reduced the number of fatty droplets in the liver. Interestingly, the expression of adiponectin and lipid oxidative enzymes significantly increased after 2 weeks of treatment. These results indicate that the expression of adiponectin and lipid oxidative enzymes in the early stages of BADGE or caffeine treatment correlated well with the long-term anti-obesity effects.
Yun
Jun-Won
JW
Amorepacific Corporation R&D Center, Daegu, Korea.
Shin
Eui-Seok
ES
Cho
Si-Young
SY
Kim
Shin-Hyoung
SH
Kim
Chae-Wook
CW
Lee
Tae-Ryong
TR
Kim
Bae-Hwan
BH
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D052242:Adiponectin / Q000032:analysis*; D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology*; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage*; D005234:Fatty Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism*; D013997:Time Factors
D052242:Adiponectin; D001713:Biphenyl Compounds; D004041:Dietary Fats; D002110:Caffeine; D002334:Carnitine O-Palmitoyltransferase; D000103:Acetyl-CoA Carboxylase