title: Kinetics of Schiff base formation between the cholesterol ozonolysis product 3-beta-hydroxy-5-oxo-5,6-secocholestan-6-al and phosphatidylethanolamine.
abstract: The kinetics of Schiff base formation between the cholesterol ozonolysis product 3beta-hydroxy-5-oxo-5,6-secocholestan-6-al and dimyristoyl phosphatidylethanolamine was investigated. The activation energy of Schiff base formation at temperatures above and below the phase transition of the phospholipid was calculated. Increase in the activation energy derived from perturbation of the surface structure by the process of Schiff base formation was demonstrated. The presence of the Schiff base with the aldolization product of the oxysterol was also observed and its significance is discussed.
authors.affiliation: Department of Biological Chemistry, The Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Bach
authors.forename: E
authors.initials: E
authors.lastname: Wachtel
authors.forename: I R
authors.initials: IR
authors.lastname: Miller
mesh_terms: D000042:Absorption; D002462:Cell Membrane / Q000737:chemistry; D002778:Cholestanones / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D005782:Gels; D007700:Kinetics; D010126:Ozone / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry*; D012632:Secosteroids / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry
chemical_list: D002778:Cholestanones; D005782:Gels; D010714:Phosphatidylethanolamines; D012545:Schiff Bases; D012632:Secosteroids; D014867:Water; C483858:phosphatidylethanolamine; D000431:Ethanol; D010126:Ozone; C082684:3-hydroxy-5-oxo-5,6-secocholestan-6-al; D002784:Cholesterol; C018525:1,2-dimyristoylphosphatidylethanolamine