title: [Study of expression of beta-III tubulin in human eye tissues during prenatal development].
abstract: Expression of beta-III tubulin, a marker protein of early neuronal cells, was studied by molecular genetic and immunochemical techniques. The study was performed with human eyes in the 8.5th to 27-28th weeks of prenatal development. Expression of beta-III tubulin was detected immunochemically in the retina and lens fibers in the 8.5 to 22-23 weeks of development. PCR revealed a high level of expression of the gene for beta-III tubulin in the retina of 9.5-week embryos. The level of expression of this gene remained high until the 18th week of prenatal development, slightly decreased to the 24th week, and became negligible in 27- to 28-week embryos. In the 15th to 24th weeks of prenatal development, the level of expression of this gene in the lens was very low and became undetectable in 27- to 28-week embryos. The results of PCR analysis are consistent with immunochemical data.
authors.forename: I G
authors.initials: IG
authors.lastname: Panova
authors.forename: Iu B
authors.initials: IuB
authors.lastname: Markitantova
authors.forename: N V
authors.initials: NV
authors.lastname: Firsova
authors.forename: O V
authors.initials: OV
authors.lastname: Podgornyĭ
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Smirnova
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
authors.forename: R D
authors.initials: RD
authors.lastname: Zinov'eva
authors.forename: V I
authors.initials: VI
authors.lastname: Mitashov
mesh_terms: D005136:Eye Proteins / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000196:embryology*; D012160:Retina / Q000166:cytology / Q000196:embryology*; D014404:Tubulin / Q000096:biosynthesis*
chemical_list: D005136:Eye Proteins; C498585:TUBB3 protein, human; D014404:Tubulin