title: [Differential transcription of Bcl-2 and Bax through the cell cycle in prostate cancer cell line].
abstract: OBJECTIVE
  To investigate the differential expression of apoptosis associated gene Bcl-2 and Bax through cell cycle and its possible clinical meaning.
  
  
  METHODS
  The prostate cancer cell line PC-3 was synchronized in M, G1, S and G2 phase using modified thymine deoxyriboside blockage and high pressure N2O technique. The efficiency of synchronization was detected by flow-cytometry. RT-PCR and Western blot methods were used to examine the expression of Bcl-2 and Bax in mRNA and protein level.
  
  
  RESULTS
  The synchronized rate of M, G1, S and G2 phase were 92.1%, 87.0%, 80.2% and 75.9% respectively. Bcl-2 was constitutively expressed through the cell cycle, but both the mRNA and protein expression level of Bcl-2 were very high in the G1 phase, dramatically decreased in M, S and G2 phase. The expression level of Bax had no change through the cell cycle.
  
  
  CONCLUSIONS
  Cell cycle could influence the expression level of Bcl-2 significantly but not Bax, these might have some clinical relevance.
authors.affiliation: Department of Urology, Institute of Urology, Peking University First Hospital, Beijing 100034, China.
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Li
authors.forename: Kan
authors.initials: K
authors.lastname: Gong
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Sun
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Zhou
authors.forename: Zhi-Song
authors.initials: ZS
authors.lastname: He
authors.forename: Dian-Qi
authors.initials: DQ
authors.lastname: Xin
authors.forename: Yan-Qun
authors.initials: YQ
authors.lastname: Na
authors.forename: Yun-Yan
authors.initials: YY
authors.lastname: Liang
authors.forename: Dai-Shu
authors.initials: DS
authors.lastname: Wang
authors.forename: Ying-Lu
authors.initials: YL
authors.lastname: Guo
mesh_terms: D002453:Cell Cycle; D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein