title: Development of nanoparticulate drug delivery systems based on thiolated poly(acrylic acid).
abstract: In this study the preparation and stabilization of poly(acrylic acid)-cysteine nanoparticles and incorporation of a fluorescence marked model-compound was investigated. Nanoparticles were prepared by ionic gelation of a poly(acrylic acid)-cysteine conjugate with calcium chloride. Poly(acrylic acid)-cysteine nanoparticles display high cohesive properties due to a cross-linking process via calcium bridges in the core and the pervasive formation of disulphide bonds and were 139 ± 34 nm in size. Nanoparticles were loaded with FITC-dextrans (flourescein isothiocyanate-dextrans) of 4, 20 and 40 kDa molecular mass as model-compound via sonication method or via vibration method for 3 and 24 h. In vitro release studies showed an initial burst release followed by an extended release of model-compounds. The lower the molecular mass of the FITC-dextrans, the higher was the amount of incorporated and released model compounds. Vibration seems to be a proper method for the incorporation of hydrophilic and macromolecular drugs in poly(acrylic acid)-cysteine nanoparticles.
authors.affiliation: ThioMatrix GmbH, Research Center, Innsbruck, Austria.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Thaurer
authors.forename: Britta
authors.initials: B
authors.lastname: Deutel
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schlocker
authors.forename: Andreas
authors.initials: A
authors.lastname: Bernkop-Schnürch
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D003911:Dextrans / Q000008:administration & dosage*; D004337:Drug Carriers / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000031:analogs & derivatives*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D003911:Dextrans; D004337:Drug Carriers; D013438:Sulfhydryl Compounds; C015219:fluorescein isothiocyanate dextran; C006903:carbopol 940; D016650:Fluorescein-5-isothiocyanate