Body surface area prediction in normal, hypermuscular, and obese mice.
BACKGROUND
Accurate determination of body surface area (BSA) in experimental animals is essential for modeling effects of burn injury or drug metabolism. Two-dimensional surface area is related to three-dimensional body volume, which in turn can be estimated from body mass. The Meeh equation relates body surface area to the two-thirds power of body mass, through a constant, k, which must be determined empirically by species and size. We found older values of k overestimated BSA in certain mice; thus we determined empirically k for various strains of normal, obese, and hypermuscular mice.


METHODS
BSA was computed from digitally scanned pelts and nonlinear regression analysis was used to determine the best-fit k.


RESULTS
The empirically determined k for C57BL/6J mice of 9.82 was not significantly different from other inbred and outbred mouse strains of normal body composition. However, mean k of the nearly spheroid, obese lepr(db/db) mice (k = 8.29) was significantly lower than for normals, as were values for dumbbell-shaped, hypermuscular mice with either targeted deletion of the myostatin gene (Mstn) (k = 8.48) or with skeletal muscle specific expression of a dominant negative myostatin receptor (Acvr2b) (k = 8.80).


CONCLUSIONS
Hypermuscular and obese mice differ substantially from normals in shape and density, resulting in considerably altered k values. This suggests Meeh constants should be determined empirically for animals of altered body composition. Use of these new, improved Meeh constants will allow greater accuracy in experimental models of burn injury and pharmacokinetics.
Cheung
Michael C
MC
Division of Surgical Oncology, University of Miami Miller School of Medicine, Miami, Florida 33136, USA.
Spalding
Paul B
PB
Gutierrez
Juan C
JC
Balkan
Wayne
W
Namias
Nicholas
N
Koniaris
Leonidas G
LG
Zimmers
Teresa A
TA
D000465:Algorithms; D000818:Animals; D001823:Body Composition; D001830:Body Surface Area; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D023421:Models, Animal; D009132:Muscles; D009765:Obesity / Q000473:pathology*; D010641:Phenotype