title: Molecular epidemiology of dengue virus serotype 2 in the Taiwan 2002 outbreak with envelope gene and nonstructural protein 1 gene analysis.
abstract: The genetic relationships among dengue virus serotype 2 (DEN-2) isolates from the Taiwan 2002 epidemic were studied by sequence analysis of the envelope (E) and nonstructural protein 1 (NS1) genes. A 0-0.4% divergence among 10 isolates revealed an epidemic strain in the outbreak. Phylogenetic study demonstrated that the 2002 Taiwan isolates were of the Cosmopolitan genotype, which is different from the Asian 1 and Asian 2 genotypes of Taiwan DEN-2 isolates from 1981 to 1998 and the American/Asian genotype of 2005 Taiwan isolates. Although grouping results from both E and NS1 gene sequence analyses were the same, the usage of the NS1 gene as a sequence analysis target has not been validated for the lower bootstrap support values of branches in the phylogenetic tree. Our result showing the same genotype changes in Taiwan and Philippines isolates suggests strain transfer of DEN-2 to nearby countries resulting in the same trend of genotype change.
authors.affiliation: Department of Public Health, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Tung
authors.forename: Kuei-Hsiang
authors.initials: KH
authors.lastname: Lin
authors.forename: Hung-Che
authors.initials: HC
authors.lastname: Chiang
authors.forename: Liang-Yin
authors.initials: LY
authors.lastname: Ke
authors.forename: Yen-Hsu
authors.initials: YH
authors.lastname: Chen
authors.forename: Guan-Ming
authors.initials: GM
authors.lastname: Ke
authors.forename: Tun-Chieh
authors.initials: TC
authors.lastname: Chen
authors.forename: Lee-Chiu
authors.initials: LC
authors.lastname: Chou
authors.forename: Po-Liang
authors.initials: PL
authors.lastname: Lu
mesh_terms: D000330:Aedes; D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004196:Disease Outbreaks; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D019595:Severe Dengue / Q000453:epidemiology* / Q000821:virology; D013624:Taiwan / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins