title: Development of novel thermal sprayed antibacterial coating and evaluation of release properties of silver ions.
abstract: Several studies have addressed the use of antibacterial coating to reduce implant-associated infections. In this study, novel silver (Ag)-containing calcium-phosphate (CP) coating technology based on the thermal spraying method was developed. The coating's physical and chemical properties, in vitro antibacterial activity, hydroxyapatite (HA)-forming ability, and release of Ag ions were evaluated. An amorphous structure of the coating was confirmed by X-ray diffraction, and Ag residue in the coating was determined by elementary analysis. The coating showed strong antibacterial activity to methicillin-resistant Staphylococcus aureus in fetal bovine serum (FBS) along with HA-forming ability in simulated body fluid. Therefore, it is expected that the coating would confer antibacterial and bone bonding abilities to the implant surface. Time course release testing of Ag ions from the coating on immersion in FBS showed pronounced Ag release for up to 24 h after immersion, with consistent strong antibacterial activity at the early postoperative stage. In repeated testing, the amount of released Ag ions was about 6500 parts per billion (ppb, microg/L) for the first release test, after which it gradually decreased. However, retention of significant release of Ag ions after a sixth repeat implies that Ag release from the coating is slow in FBS.
authors.affiliation: Research Department, Japan Medical Materials Corporation, Osaka 532-0003, Japan.
authors.forename: Iwao
authors.initials: I
authors.lastname: Noda
authors.affiliation: Research Department, Japan Medical Materials Corporation, Osaka 532-0003, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Miyaji
authors.affiliation: Research Department, Japan Medical Materials Corporation, Osaka 532-0003, Japan.|Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Ando
authors.affiliation: Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyamoto
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Shimazaki
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yonekura
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Miyazaki
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Mawatari
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Hotokebuchi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D007477:Ions / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D007477:Ions; D012710:Serum Albumin, Bovine; D012834:Silver; D017886:Durapatite