title: Pyrrospirones A and B, apoptosis inducers in HL-60 cells, from an endophytic fungus, Neonectria ramulariae Wollenw KS-246.
abstract: Pyrrospirones A and B have been isolated from unpolished rice cultures of the endophytic fungus Neonectria ramulariae Wollenw KS-246. Their absolute stereostructures (1 and 2) were elucidated through spectroscopic methods using 1D and 2D NMR techniques and chemical transformations, including the modified Mosher's method. The compounds exhibited cytotoxicity and induced apoptosis in human promyelocytic leukemia HL-60 cells.
authors.affiliation: Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University, Wakabamachi 1-23, Tsuruoka, Yamagata 997-8555, Japan. yshiono@tds1.tr.yamagata-u.ac.jp
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Shiono
authors.forename: Keiko
authors.initials: K
authors.lastname: Shimanuki
authors.forename: Fuminori
authors.initials: F
authors.lastname: Hiramatsu
authors.forename: Takuya
authors.initials: T
authors.lastname: Koseki
authors.forename: Murayama
authors.initials: M
authors.lastname: Tetsuya
authors.forename: Nozomi
authors.initials: N
authors.lastname: Fujisawa
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kimura
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D006999:Hypocreales / Q000737:chemistry*; D007564:Japan; D015394:Molecular Structure; D012275:Oryza / Q000382:microbiology; D013141:Spiro Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D013141:Spiro Compounds; C534099:pyrrospirone A; C534100:pyrrospirone B