Secretion of five extracellular enzymes by strains of chromoblastomycosis agents.
The gelatinase, urease, lipase, phospholipase and DNase activities of 11 chromoblastomycosis agents constituted by strains of Fonsecaea pedrosoi, F. compacta, Phialophora verrucosa, Cladosporium carrionii, Cladophialophora bantiana and Exophiala jeanselmei were analyzed and compared. All strains presented urease, gelatinase and lipase activity. Phospholipase activity was detected only on five of six strains of F. pedrosoi. DNase activity was not detected on the strains studied. Our results indicate that only phospholipase production, induced by egg yolk substrate, was useful for the differentiation of the taxonomically related species studied, based on their enzymatic profile.
Souza
Thais Furtado de
TF
Department of Microbiology, Institute of Basic Health Sciences, Universidade Federal do Rio Grande do Sul, Rio Grande do Sul, Brazil.
Scroferneker
Maria Lúcia
ML
Costa
Juliana Mônica da
JM
Carissimi
Mariana
M
Corbellini
Valeriano Antonio
VA
D002862:Chromoblastomycosis / Q000382:microbiology*; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D003904:Mitosporic Fungi / Q000145:classification / Q000201:enzymology*
D006867:Hydrolases