title: Rosiglitazone suppresses gastric carcinogenesis by up-regulating HCaRG expression.
abstract: Our previous study demonstrated that PPARgamma ligand rosiglitazone prevents gastric carcinogenesis in rats induced by chemical carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). In this study, we attempted to identify novel anti-cancer mechanisms of rosiglitazone. By examining the gene expression profiles of MNNG-induced and rosiglitazone-treated gastric cancer with Uniset Rat I Bioarray microarray, we identified a gene that showed prominent responses in the rosiglitazone-treated group. The hypertension-related, calcium-regulated gene (HCaRG) was significantly up-regulated in rat gastric carcinoma of the rosiglitazone-treated group when compared with the MNNG group. We further examined HCaRG expression in human gastric cancer and found that the expression of HCaRG was down-regulated in human gastric cancerous tissue. Rosiglitazone markedly induced the expression of HCaRG in the AGS cell line. The up-regulation of HCaRG may be one of the mechanisms underlying the chemopreventive effect of rosiglitazone in gastric cancer.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
authors.forename: Bai-Li
authors.initials: BL
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Zeng
authors.forename: Wai-Kit
authors.initials: WK
authors.lastname: Chu
authors.forename: Christine Y P
authors.initials: CY
authors.lastname: Wong
authors.forename: Yuen-Yee
authors.initials: YY
authors.lastname: Cheng
authors.forename: Joseph J Y
authors.initials: JJ
authors.lastname: Sung
authors.forename: Pin-Jin
authors.initials: PJ
authors.lastname: Hu
authors.forename: Wai K
authors.initials: WK
authors.lastname: Leung
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077154:Rosiglitazone; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D045162:Thiazolidinediones / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D000970:Antineoplastic Agents; D018797:Cell Cycle Proteins; C415469:Commd5 protein, rat; D009687:Nuclear Proteins; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D008769:Methylnitronitrosoguanidine