title: Delay in maturation of the submandibular gland in Chagas disease correlates with lower DNA synthesis.
abstract: It has been demonstrated that the acute phase of Trypanosoma cruzi infection promotes several changes in the oral glands. The present study examined whether T. cruzi modulates the expression of host cell apoptotic or mitotic pathway genes. Rats were infected with T. cruzi then sacrificed after 18, 32, 64 or 97 days, after which the submandibular glands were analyzed by immunohistochemistry. Immunohistochemical analyses using an anti-bromodeoxyuridine antibody showed that, during acute T. cruzi infection, DNA synthesizing cells in rat submandibular glands were lower than in non-infected animals (p < 0.05). However, after 64 days of infection (chronic phase), the number of immunolabeled cells are similar in both groups. However, immunohistochemical analysis of Fas and Bcl-2 expression did not find any difference between infected and non-infected animals in both the acute and chronic stages. These findings suggest that the delay in ductal maturation observed at the acute phase of Chagas disease is correlated with lower expression of DNA synthesis genes, but not apoptotic genes.
authors.affiliation: Laboratório de Biologia Molecular, Universidade de Uberaba, Uberaba, MG, Brasil. propg.pr@uniube.br
authors.forename: José B
authors.initials: JB
authors.lastname: Alves
authors.forename: Marcelo H
authors.initials: MH
authors.lastname: Napimoga
authors.forename: Marcelo S
authors.initials: MS
authors.lastname: Araujo
authors.forename: Gerluza A B
authors.initials: GA
authors.lastname: Silva
mesh_terms: D000818:Animals; D017209:Apoptosis; D001973:Bromodeoxyuridine / Q000378:metabolism; D014355:Chagas Disease / Q000378:metabolism / Q000473:pathology*; D004247:DNA / Q000096:biosynthesis*; D053222:Fas Ligand Protein / Q000096:biosynthesis; D007150:Immunohistochemistry; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051381:Rats; D013363:Submandibular Gland / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D013997:Time Factors
chemical_list: D053222:Fas Ligand Protein; D019253:Proto-Oncogene Proteins c-bcl-2; D004247:DNA; D001973:Bromodeoxyuridine