title: Screening and identification of a novel B-cell neutralizing epitope from Helicobacter pylori UreB.
abstract: Urease plays a crucial role in the survival and pathogenesis of Helicobacter pylori (H. pylori), and antibody neutralizing the urease activity may be implicated for the protection against H. pylori infection. Previously, a neutralizing monoclonal antibody (MAb) 6E6 against UreB of H. pylori was developed. In this work, we try to identify the B-cell epitope recognized by neutralizing MAb 6E6. Following screening a series of truncated proteins of UreB, an epitope was primarily localized in the aa 200-230 of UreB. Subsequently, we screened the overlapping synthetic peptides covering the aa 200-230 and identified a novel B-cell epitope (U(211-225), IEAGAIGFKIHEDWG) that was recognized by specific MAb 6E6. The newly identified epitope may help understanding of the protective immunity against H. pylori and be implicated for vaccine development.
authors.affiliation: Department of Clinical Microbiology and Clinical Immunology, The Third Military Medical University, Chongqing 400038, People's Republic of China.
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Li
authors.forename: Xu-Hu
authors.initials: XH
authors.lastname: Mao
authors.forename: Yun
authors.initials: Y
authors.lastname: Shi
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Wu
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Luo
authors.forename: Shu
authors.initials: S
authors.lastname: Yu
authors.forename: Wei-Ying
authors.initials: WY
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Quan-Ming
authors.initials: QM
authors.lastname: Zou
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D015153:Blotting, Western; D004353:Drug Evaluation, Preclinical; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D016481:Helicobacter Infections / Q000276:immunology; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D008968:Molecular Conformation; D009500:Neutralization Tests; D011487:Protein Conformation; D014510:Urease / Q000037:antagonists & inhibitors / Q000276:immunology*
chemical_list: D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines; D000939:Epitopes; D014510:Urease