title: Effect of rhBMP-2 on tibial plateau fractures in a canine model.
abstract: This study was to determine the efficacy of recombinant human bone morphogenetic protien-2 (rhBMP-2)/calcium phosphate matrix (CPX) paste to accelerate healing in a canine articular fracture model with associated subchondral defect. rhBMP-2/CPX (BMP), CPX alone (CPX) or autogenous bone graft (ABG) was administered to a canine articular tibial plateau osteotomy with a subchondral defect in each of 21 female dogs. The unoperated contralateral limbs served as controls. Ground reaction forces, synovial fluid, radiographic changes, mechanical testing, bone density, and histology of bone and synovium were analyzed at 6 weeks after surgery. Radiographic analysis demonstrated that the BMP and CPX groups showed improved bony healing compared to the ABG group at week 6. Histomorphometric analysis demonstrated that the BMP group had significantly increased trabecular bone volume compared to the CPX and ABG groups. Mechanical testing revealed that the BMP group had significantly greater maximum failure loads than the ABG group. Histological analysis demonstrated that the BMP group had significantly less sub-synovial inflammation than CPX group. This study demonstrated that rhBMP-2/CPX accelerated healing of articular fractures with subchondral defect compared to ABG in most of the parameters evaluated, and had less subsynovial inflammation than the CPX alone in a canine model.
authors.affiliation: Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Susan L
authors.initials: SL
authors.lastname: Schaefer
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Howard
authors.initials: H
authors.lastname: Seeherman
authors.forename: X Jian
authors.initials: XJ
authors.lastname: Li
authors.forename: Mandi J
authors.initials: MJ
authors.lastname: Lopez
authors.forename: Mark D
authors.initials: MD
authors.lastname: Markel
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000627:therapeutic use; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D017102:Fracture Healing / Q000187:drug effects; D006801:Humans; D011994:Recombinant Proteins / Q000627:therapeutic use; D013978:Tibial Fractures / Q000188:drug therapy* / Q000473:pathology
chemical_list: D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D011994:Recombinant Proteins; C020243:calcium phosphate