Simultaneous spectrophotometric estimation of Bisoprolol fumarate and hydrochlorothiazide in tablet dosage form.
Two analytical methods have been developed for simultaneous quantification of bisoprolol fumarate and hydrochlorothiazide in combined pharmaceutical dosage form using spectrophotometer. Excellent simplicity, accuracy, precision and economy were achieved by the assay. The method I was based upon simultaneous equations whereas the method II was based upon multicomponent mode of analysis of the instrument. For both these methods, 0.1 N NaOH was used as solvent. In this solvent, bisoprolol fumarate showed absorbance at 224 nm and hydrochlorothiazide at 273 nm. Linearity lies in the concentration range of 3-21 microg/ ml for bisoprolol fumarate and 3-18 microg/ ml for hydrochlorothiazide. The methods were validated statistically and by recovery studies.
A
Shirkhedkar Atul
SA
RC Patel College of Pharmacy, Karwand Naka, Shirpur, 425 405, Dist. Dhule (MS), India. atul_shirkhedkar@yahoo.com
R
Thorve Rajesh
TR
J
Surana Sanjay
SS
D000959:Antihypertensive Agents / Q000032:analysis*; D017298:Bisoprolol / Q000032:analysis*; D004338:Drug Combinations; D006852:Hydrochlorothiazide / Q000032:analysis*; D015203:Reproducibility of Results; D013053:Spectrophotometry; D013607:Tablets; D013678:Technology, Pharmaceutical / Q000379:methods*
D000959:Antihypertensive Agents; D004338:Drug Combinations; D013607:Tablets; D006852:Hydrochlorothiazide; D017298:Bisoprolol