title: Validation of EST-derived STS markers localized on Qfhs.ndsu-3BS for Fusarium head blight resistance in wheat using a 'Wangshuibai' derived population.
abstract: A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight (FHB) in wheat, have been previously identified in the 'Sumai 3'/'Stoa' population. In this study, we used a 'Wangshuibai' (resistant) /'Seri82' (susceptible) derived population, linkage group, QTL, and quantitative gene expression analysis to assess the genetic background dependence and stability of the EST-derived STS markers for use in marker aided selection to improve FHB resistance in wheat. Based on our results, a QTL in the map interval of Xsts3B-138_1-Xgwm493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16% of the phenotypic variation. BLASTN analysis indicated that Xsts3B-138_1 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 in 'Wangshuibai' at 96 h after inoculation was 2.6 times higher than 'Seri82'. Our results underlined that EST-derived STS3B-138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat.
authors.affiliation: Department of Agronomy and Crop Breeding, Shahed University of Tehran, Tehran 3319118651, Iran.
authors.forename: Amir Mohammad
authors.initials: AM
authors.lastname: Naji
authors.forename: Mohammad
authors.initials: M
authors.lastname: Moghaddam
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ghaffari
authors.forename: Hashem Pour
authors.initials: HP
authors.lastname: Irandoost
authors.forename: Laleh Karimi
authors.initials: LK
authors.lastname: Farsad
authors.forename: Seyed Mostafa
authors.initials: SM
authors.lastname: Pirseyedi
authors.forename: Seyed Abolghasem
authors.initials: SA
authors.lastname: Mohammadi
authors.forename: Behzad
authors.initials: B
authors.lastname: Ghareyazie
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mardi
mesh_terms: D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D005670:Fusarium / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D010935:Plant Diseases / Q000382:microbiology*; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci / Q000235:genetics*; D016324:Sequence Tagged Sites; D014908:Triticum / Q000145:classification / Q000235:genetics* / Q000382:microbiology*
chemical_list: D005819:Genetic Markers