title: Laboratory study of a complex translocation t(2;8;21) (p12;q22;q22) in a patient with acute myelogenous leukemia.
abstract: The t(2;8;21) is a complex translocation of t(8;21), and is very rare. To investigate the laboratory characteristics of a complex translocation t(2;8;21)(p12;q22;q22) in a patient with acute myelogenous leukemia (AML-M2), bone marrow smears were prepared for morphological analysis. Bone marrow samples were collected for FCM, and prepared by short-term (24 h) unstimulated culture for R-binding and conventional cytogenetic assay (CCA). AML1/ETO chimera transcription was detected by reverse transcriptase polymerase chain reaction (RT-PCR), and laboratory test results were studied with multifactorial analysis method. In this case, the chromosomal analysis (R-banding) demonstrated a complex translocation t(2;8;21)(p12;q22;q22). With combined evidence from morphological and immunophenotypic results, the patient was diagnosed as AML-M2. t(2;8;21)(p12;q22;q22) was considered a rare complex translocation of t(8;21). Experience of the present case further emphasises the importance of the laboratory diagnostic methods.
authors.affiliation: Hematology Laboratory, Hematology Malignancy Treatment Center, Shengjing Hospital, China Medical University, Shenyang, China. Zhangjihong_cmu@Sina.com
authors.forename: Jihong
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhuogang
authors.initials: Z
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Shao
authors.forename: Yu
authors.initials: Y
authors.lastname: Ma
authors.forename: Haixia
authors.initials: H
authors.lastname: Tong
authors.forename: Yunxiu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D001856:Bone Marrow Examination; D002889:Chromosomes, Human, Pair 2; D002891:Chromosomes, Human, Pair 21; D002898:Chromosomes, Human, Pair 8; D050676:Core Binding Factor Alpha 2 Subunit / Q000032:analysis; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000032:analysis; D000075142:RUNX1 Translocation Partner 1 Protein; D014178:Translocation, Genetic
chemical_list: C107844:AML1-ETO fusion protein, human; D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; D000075142:RUNX1 Translocation Partner 1 Protein