title: An association study of 13 SNPs from seven candidate genes with pediatric asthma and a preliminary study for genetic testing by multiple variants in Taiwanese population.
abstract: BACKGROUND
  Asthma is one of the most common chronic diseases in children. It is caused by complex interactions between various genetic factors and exposures to environmental allergens and irritants. Because of the heterogeneity of the disease and the genetic and cultural differences among different populations, a proper association study and genetic testing for asthma and susceptibility genes is difficult to perform.
  
  
  METHODS
  We assessed 13 single-nucleotide polymorphisms (SNPs) in seven well-known asthma susceptibility genes and looked for association with pediatric asthma using 449 asthmatic subjects and 512 non-asthma subjects in Taiwanese population.
  
  
  RESULTS
  CD14-159 C/T and MS4A2 Glu237Gly were identified to have difference in genotype/allele frequencies between the control group and asthma patients. Moreover, the genotype synergistic analysis showed that the co-contribution of two functional SNPs was riskier or more protective from asthma attack. Our study provided a genotype synergistic method for studying gene-gene interaction on polymorphism basis and genetic testing using multiple polymorphisms.
authors.affiliation: Department of Pediatrics and Institute of Molecular Medicine, College of Medicine, National Cheng-Kung University, Tainan, Taiwan.
authors.forename: Jiu-Yao
authors.initials: JY
authors.lastname: Wang
authors.forename: Ya-Huei
authors.initials: YH
authors.lastname: Liou
authors.forename: Ying-Jye
authors.initials: YJ
authors.lastname: Wu
authors.forename: Ya-Hsin
authors.initials: YH
authors.lastname: Hsiao
authors.forename: Lawrence Shih-Hsin
authors.initials: LS
authors.lastname: Wu
mesh_terms: D001249:Asthma / Q000453:epidemiology* / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D013624:Taiwan / Q000453:epidemiology