The effective and specific isolation of antibodies from human serum by using thiophilic paramagnetic polymer beads.
A rapid and effective method to specifically isolate immunoglobulin G (IgG) from human serum by thiophilic paramagnetic polymer beads was developed. The thiophilic paramagnetic beads were synthesized with vinyl acetate and divinylbenzene by microsuspension polymerization in the presence of magnetite nanoparticles. Divinylsulfone and 2-mercapto-benzothiazole were subsequently used to modify the surface of these beads, resulting in thiophilic particles that exhibited a high specificity to the antibodies in serum at low salt concentration. The adsorbed IgG was eluted by 0.8M KCl and 72% of the IgG in the serum was recovered. The purity of the isolated IgG reached 98.4% and the bioactivity was fully maintained (>99%). The high efficiency, mild conditions and simplicity make this technology suitable for the economic purification of antibodies in a large scale.
Qian
Hao
H
College of Material Science and Engineering, Huaqiao University, Quanzhou 362021, Fujian, China. hquqh@yahoo.com.cn
Chen
Ming Qin
MQ
Lin
Zhi Yong
ZY
D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013830:Thiadiazoles / Q000737:chemistry
D007074:Immunoglobulin G; D011108:Polymers; D013438:Sulfhydryl Compounds; D013830:Thiadiazoles