Sequence and analysis of a plasmid-encoded mercury resistance operon from Mycobacterium marinum identifies MerH, a new mercuric ion transporter.
In this study, we report the DNA sequence and biological analysis of a mycobacterial mercury resistance operon encoding a novel Hg(2+) transporter. MerH was found to transport mercuric ions in Escherichia coli via a pair of essential cysteine residues but only when coexpressed with the mercuric reductase.
Schué
Mathieu
M
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom. matschue@yahoo.fr
Dover
Lynn G
LG
Besra
Gurdyal S
GS
Parkhill
Julian
J
Brown
Nigel L
NL
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D017124:Conserved Sequence; D017931:DNA Primers; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D017669:Mercury Compounds / Q000378:metabolism* / Q000494:pharmacology*; D008969:Molecular Sequence Data; D019910:Mycobacterium marinum / Q000187:drug effects* / Q000235:genetics*; D009876:Operon / Q000235:genetics*; D010957:Plasmids; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D017931:DNA Primers; D017669:Mercury Compounds