title: [Improvement of the silver-stained technique of polyacrylamide gel electrophoresis.].
abstract: Polyacrylamide gel electrophoresis is widely applied in the SSR, SNP markers analysis and the construction of genetic map. It is disadvantage to use conventional silver-stained methods in experiment due to taking a very long time and having fussy steps. In this paper, a silver-stained method of DNA in PAGE gel was improved. The improved method is rapid banding which only takes about 20 min, and the high definition picture was obtained.
authors.affiliation: 1. Key Laboratory of Fish Germplasm Resources and Biotechnology Certificated by Ministry of Agriculture,Yangtze River Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China; 2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; E-mail:lianghw@yfi.ac.cn.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liang
authors.forename: Chang-Zhong
authors.initials: CZ
authors.lastname: Wang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.forename: Xiang-Zhong
authors.initials: XZ
authors.lastname: Luo
authors.forename: Gui-Wei
authors.initials: GW
authors.lastname: Zou
mesh_terms: D004247:DNA; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D012834:Silver; D016622:Silver Staining; D013194:Staining and Labeling
chemical_list: D012834:Silver; D004247:DNA