Synthesis and use of pHEMA microbeads with human EA.hy 926 endothelial cells.
Cancer has become a major problem in public health and the resulting bone metastases a worsening factor. Facing it, different strategies have been proposed and mechanisms involved in tumor angiogenesis are being studied. Enhanced permeability retention (EPR) effect is a key step in designing new anticancer drugs. We have prepared poly 2-hydroxyethyl methacrylate (pHEMA) microbeads to target human endothelial EA.hy 926 cells, a cell line derived from human umbilical vein endothelial cells. Microbeads were synthesized by emulsion precipitation method and carried positive or negative charges. EA.hy 926 cells were cultured in 24-well plates and microbeads were deposited on cells at various times. Scanning and transmission electron microscopy, flow cytometry, confocal microscopy, and three-dimensional (3D) reconstruction were used to characterize microbeads and their location outside and inside cells. Microbeads were uptaken by endothelial cells with a better internalization for negatively charged microbeads. 3D reconstruction of confocal optical sections clearly evidenced the uptake and internalization of microbeads by endothelial cells. pHEMA microbeads could represent potential drug carrier in tumor model of metastases.
Nyangoga
Hervé
H
INSERM, U922-LHEA, Faculté de Médecine, 49045 Angers Cedex, France.
Zecheru
Teodora
T
INSERM, U922-LHEA, Faculté de Médecine, 49045 Angers Cedex, France.|Department of Macromolecular Compounds, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Bucharest 010072, Romania.
Filmon
Robert
R
INSERM, U922-LHEA, Faculté de Médecine, 49045 Angers Cedex, France.
Baslé
Michel-Félix
MF
INSERM, U922-LHEA, Faculté de Médecine, 49045 Angers Cedex, France.
Cincu
Corneliu
C
INSERM, U922-LHEA, Faculté de Médecine, 49045 Angers Cedex, France.|Department of Macromolecular Compounds, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Bucharest 010072, Romania.
Chappard
Daniel
D
INSERM, U922-LHEA, Faculté de Médecine, 49045 Angers Cedex, France.
D001672:Biocompatible Materials; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D008863:Microspheres; D015394:Molecular Structure; D009369:Neoplasms / Q000628:therapy; D009389:Neovascularization, Pathologic
D001672:Biocompatible Materials; D004337:Drug Carriers; D008689:Methacrylates; C005044:hydroxyethyl methacrylate