Co-localization of hyperphosphorylated tau and caspases in the brainstem of Alzheimer's disease patients.
Hyperphosphorylation of microtubule associated protein tau had limited studies in Alzheimer's disease (AD) brainstem. We compared the distribution and number of neurons with hyperphosphorylated tau in two age groups of AD brainstems with mean ages of 65.4 +/- 5.7 and 91.1 +/- 6.4 years. The degree of co-localization of hyperphosphorylated tau positive cells with either cleaved caspase-3 or cleaved caspase-6 was also quantified. Results showed hyperphosphorylated tau mainly occurred in hypoglossal, dorsal motor vagal, trigeminal sensory/motor nuclei as well as in dorsal raphe, locus coeruleus and substantia nigra. Older AD brainstem consistently had higher density of hyperphosphorylated tau cells. Up to 70% of tau positive cells also displayed either cleaved caspase-3 or caspase-6, and the number of co-localized tau cells in each caspase subfamily group was always higher in older aged group. Some hyperphosphorylated tau cells with cleaved caspases had TUNEL positive nuclei. These findings suggest that these latter cells went through the apoptotic process or DNA fragmentation.
Wai
Maria Sen Mun
MS
Department of Anatomy, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
Liang
Yong
Y
Shi
Chun
C
Cho
Eric Y P
EY
Kung
Hsiang-Fu
HF
Yew
David T
DT
D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism; D000544:Alzheimer Disease / Q000201:enzymology* / Q000473:pathology; D017209:Apoptosis; D001933:Brain Stem / Q000201:enzymology* / Q000473:pathology; D053148:Caspase 3 / Q000032:analysis*; D053178:Caspase 6 / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008875:Middle Aged; D009474:Neurons / Q000201:enzymology* / Q000473:pathology; D010766:Phosphorylation; D012694:Serine; D016875:tau Proteins / Q000032:analysis*
C054369:MAPT protein, human; D016875:tau Proteins; D012694:Serine; C505407:CASP3 protein, human; C505514:CASP6 protein, human; D053148:Caspase 3; D053178:Caspase 6