Deciphering the complete mitochondrial genome and phylogeny of the extinct cave bear in the Paleolithic painted cave of Chauvet.
Retrieving a large amount of genetic information from extinct species was demonstrated feasible, but complete mitochondrial genome sequences have only been deciphered for the moa, a bird that became extinct a few hundred years ago, and for Pleistocene species, such as the woolly mammoth and the mastodon, both of which could be studied from animals embedded in permafrost. To enlarge the diversity of mitochondrial genomes available for Pleistocene species, we turned to the cave bear (Ursus spelaeus), whose only remains consist of skeletal elements. We collected bone samples from the Paleolithic painted cave of Chauvet-Pont d'Arc (France), which displays the earliest known human drawings, and contains thousands of bear remains. We selected a cave bear sternebra, radiocarbon dated to 32,000 years before present, from which we generated overlapping DNA fragments assembling into a 16,810-base pair mitochondrial genome. Together with the first mitochondrial genome for the brown bear western lineage, this study provides a statistically secured molecular phylogeny assessing the cave bear as a sister taxon to the brown bear and polar bear clade, with a divergence inferred to 1.6 million years ago. With the first mitochondrial genome for a Pleistocene carnivore to be delivered, our study establishes the Chauvet-Pont d'Arc Cave as a new reservoir for Paleogenetic studies. These molecular data enable establishing the chronology of bear speciation, and provide a helpful resource to rescue for genetic analysis archeological samples initially diagnosed as devoid of amplifiable DNA.
Bon
Céline
C
Service de Biologie Intégrative et Génétique Moléculaire, Institut de Biologie et Technologies de Saclay, Commissariat à l'Energie Atomique Saclay, 91191 Gif-sur-Yvette Cedex, France.
Caudy
Nicolas
N
de Dieuleveult
Maud
M
Fosse
Philippe
P
Philippe
Michel
M
Maksud
Frédéric
F
Beraud-Colomb
Eliane
E
Bouzaid
Eric
E
Kefi
Rym
R
Laugier
Christelle
C
Rousseau
Bernard
B
Casane
Didier
D
van der Plicht
Johannes
J
Elalouf
Jean-Marc
JM
D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D001842:Bone and Bones / Q000737:chemistry*; D016000:Cluster Analysis; D004272:DNA, Mitochondrial / Q000235:genetics*; D053476:Extinction, Biological; D005602:France; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D001503:Ursidae / Q000145:classification / Q000235:genetics*
D004272:DNA, Mitochondrial