title: Factors associated with peripheral blood stem cell yield in volunteer donors mobilized with granulocyte colony-stimulating factors: the impact of donor characteristics and procedural settings.
abstract: Peripheral blood stem cells (PBSCs) are increasingly used as the source of hematopoietic stem cells, but there are large variations in harvest outcome between individuals mobilized by granulocyte colony-stimulating factor (G-CSF). We examined the effects of donor characteristics and procedure factors on the day 1 CD34+ cell yield in 373 unrelated healthy donors. G-CSF was administered subcutaneously at a planned dose of 8.3 to 11 microg/kg daily for 5 days, followed by harvest started on day 5 of G-CSF treatment. Of the 373 donors, 159 (42.6%) had the radial artery as the inlet access for harvest. Poor day 1 cell yield was defined as <10x10(6) CD34+ cells/L of processed blood for the first apheresis; 62 donors (16.6%) did not attain this threshold. The male donors had significantly higher yields at harvest compared with the female donors. The female donors had higher CD34+ cell yields if the circulation access was through an artery than if is was through a vein. In a multiple regression analysis, donor age, sex, body mass index (BMI), preharvest white blood cell and circulating immature cell counts, access type, and flow rate correlated with day 1 yield. Female sex, older age, venous access, and a higher flow rate were significantly associated with greater risk for a day 1 poor yield of CD34+ cells (odds ratio=3.0074, 1.045, 4.3362, and 1.1131, respectively). A higher BMI may decrease the risk (odds ratio=0.8472). In donors at higher risk for poor CD34+ cell yield, strategies for increasing CD34+ cells must be considered.
authors.affiliation: Department of Hematology and Oncology, Tzu-Chi General Hospital, and Department of Public Health, College of Medicine, Tzu-Chi University, Hualien, Taiwan.
authors.forename: Tso-Fu
authors.initials: TF
authors.lastname: Wang
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Wen
authors.forename: Rong-Long
authors.initials: RL
authors.lastname: Chen
authors.forename: Chi-Jui
authors.initials: CJ
authors.lastname: Lu
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Zheng
authors.forename: Shang-Hsien
authors.initials: SH
authors.lastname: Yang
authors.forename: Sung-Chao
authors.initials: SC
authors.lastname: Chu
authors.forename: Ruey-Ho
authors.initials: RH
authors.lastname: Kao
authors.forename: Shu-Huey
authors.initials: SH
authors.lastname: Chen
mesh_terms: D000328:Adult; D018952:Antigens, CD34; D015992:Body Mass Index; D002469:Cell Separation; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells; D006801:Humans; D007958:Leukocyte Count; D019520:Living Donors; D008297:Male; D012727:Sex Characteristics; D012737:Sex Factors
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor