title: Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism.
abstract: We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run using these primers, products were denatured and single-strand DNA fragments were subjected to electrophoresis. After silver staining, ssDNA band patterns characteristic for each genotype were observed. Minor differences within a serotype were detected without need for sequencing the PCR products making the preliminary analysis of avian influenza isolates much faster.
authors.affiliation: Department of Molecular Virology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, Gdansk, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Gromadzka
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Smietanka
authors.forename: Jakub
authors.initials: J
authors.lastname: Dragun
authors.forename: Zenon
authors.initials: Z
authors.lastname: Minta
authors.forename: Anna
authors.initials: A
authors.lastname: Gora-Sochacka
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Szewczyk
mesh_terms: D000818:Animals; D001717:Birds / Q000821:virology; D018076:DNA, Complementary / Q000235:genetics; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D005585:Influenza in Birds / Q000145:classification / Q000175:diagnosis / Q000235:genetics; D010802:Phylogeny; D018807:Polymorphism, Single-Stranded Conformational; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013696:Temperature
chemical_list: D018076:DNA, Complementary