Construction of an encapsulated ESAT-6-based anti-TB DNA vaccine and evaluation of its immunogenic properties.
Experimental preparations based on a DNA vaccine encoding the ESAT-6 antigen of Mycobacterium tuberculosis have been obtained (KpONE6) and studied for immunogenic effects in the murine model. The core of the preparation contains DNA of the recombinant plasmid pONE6 encapsulated within a spermidine-polyglucin conjugate, thereby protecting the DNA vaccine from degradation. KpONE6 induces a proliferative T-cell immune response in mice upon intramuscular immunization.
Nosareva
Olesya
O
FSRI State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance in Consumer Rights Protection and Human Well-being, Novosibirsk Region, 630559 Koltsovo, Russia. nosarevao@mail.ru
Nesterov
Andrey
A
Boldyrev
Alexander
A
Smirnova
Olga
O
Tumanov
Yurii
Y
Kouzmitcheva
Galina
G
Tatkov
Sergey
S
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D049109:Cell Proliferation; D016207:Cytokines / Q000096:biosynthesis; D004339:Drug Compounding; D004797:Enzyme-Linked Immunosorbent Assay; D005057:Eukaryotic Cells / Q000276:immunology; D007273:Injections, Intramuscular; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D023282:Nuclease Protection Assays; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D011134:Polysaccharides / Q000276:immunology; D013095:Spermidine / Q000276:immunology; D032581:Tuberculosis Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D011134:Polysaccharides; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D007371:Interferon-gamma; D013095:Spermidine