title: Purification, crystallization and preliminary X-ray diffraction analysis of the CBS-domain pair from the Methanococcus jannaschii protein MJ0100.
abstract: CBS domains are small protein motifs consisting of a three-stranded beta-sheet and two alpha-helices that are present in proteins of all kingdoms of life and in proteins with completely different functions. Several genetic diseases in humans have been associated with mutations in their sequence, which has made them promising targets for rational drug design. The C-terminal domain of the Methanococcus jannaschii protein MJ0100 includes a CBS-domain pair and has been overexpressed, purified and crystallized. Crystals of selenomethionine-substituted (SeMet) protein were also grown. The space group of both the native and SeMet crystals was determined to be orthorhombic P2(1)2(1)2(1), with unit-cell parameters a = 80.9, b = 119.5, c = 173.3 A. Preliminary analysis of the X-ray data indicated that there were eight molecules per asymmetric unit in both cases.
authors.affiliation: Structural Biology Unit, CIC bioGUNE, Parque Tecnológico de Bizkaia, Edificio 800, 48160 Derio, Bizkaia, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Lucas
authors.forename: Danel
authors.initials: D
authors.lastname: Kortazar
authors.forename: Egoitz
authors.initials: E
authors.lastname: Astigarraga
authors.forename: José A
authors.initials: JA
authors.lastname: Fernández
authors.forename: Jose M
authors.initials: JM
authors.lastname: Mato
authors.forename: María Luz
authors.initials: ML
authors.lastname: Martínez-Chantar
authors.forename: Luis Alfonso
authors.initials: LA
authors.lastname: Martínez-Cruz
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017124:Conserved Sequence; D003460:Crystallization; D017931:DNA Primers; D017017:Methanococcus / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014961:X-Ray Diffraction
chemical_list: D019843:Archaeal Proteins; D017931:DNA Primers