Microarray analysis of micro-ribonucleic acid expression in primary immunoglobulin A nephropathy.
OBJECTIVE
To explore the relationship between immunoglobulin A nephropathy (IgAN) and microRNA (miRNA).


METHODS
We analyzed the miRNA expression profiles in renal biopsies from 11 IgAN patients and 3 controls at the Kidney Transplantation and Hemo Purification Center of 181 Hospital, China, from May to October 2007, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences.


RESULTS
This study identified 132 miRNAs in renal samples, of which 35 miRNAs up-regulated in IgAN biopsies. The chip results were confirmed by northern blot analysis and by quantitative real-time polymerase chain reaction (RT-PCR) tests.


CONCLUSIONS
Our study may help clarify the molecular mechanisms involved in the pathogenesis of IgAN, and miRNAs potentially serve as a novel diagnostic biomarker of IgAN.
Dai
Yong
Y
Clinical Medical Research Center, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China.
Sui
Weiguo
W
Lan
Huijuan
H
Yan
Qiang
Q
Huang
He
H
Huang
Yuanshuai
Y
D000293:Adolescent; D000328:Adult; D044466:Asian People / Q000235:genetics; D001706:Biopsy; D016022:Case-Control Studies; D002681:China; D005260:Female; D005922:Glomerulonephritis, IGA / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007668:Kidney / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis
D035683:MicroRNAs