title: Peptide-based SAMs that resist the adsorption of proteins.
abstract: In this paper we present a modular approach for the fabrication of surfaces to characterize protein-protein interactions. The approach is based on azido peptides with an optimized sequence which are then thiol-functionalized using an alkynyl thiol and "click" chemistry. From these peptide thiols we fabricated SAMs on gold to evaluate the protein resistance, using surface plasmon resonance spectroscopy, toward streptavidin, bovin serum albumin (BSA), and fibronectin.
authors.affiliation: Physikalische Chemie 1 and Anorganische Chemie I, Ruhr-Universität Bochum, 44780 Bochum, Germany.
authors.forename: Rolf
authors.initials: R
authors.lastname: Chelmowski
authors.forename: Stephan David
authors.initials: SD
authors.lastname: Köster
authors.forename: Andreas
authors.initials: A
authors.lastname: Kerstan
authors.forename: Andreas
authors.initials: A
authors.lastname: Prekelt
authors.forename: Christian
authors.initials: C
authors.lastname: Grunwald
authors.forename: Tobias
authors.initials: T
authors.lastname: Winkler
authors.forename: Nils
authors.initials: N
authors.lastname: Metzler-Nolte
authors.forename: Andreas
authors.initials: A
authors.lastname: Terfort
authors.forename: Christof
authors.initials: C
authors.lastname: Wöll
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D001386:Azides / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D001386:Azides; D001672:Biocompatible Materials; D005353:Fibronectins; D010455:Peptides; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D019809:Streptavidin