title: Genetic variability in the 5' UTR and NS5A regions of hepatitis C virus RNA isolated from non-responding and responding patients with chronic HCV genotype 1 infection.
abstract: Sequence variation among different hepatitis C virus (HCV) isolates has adaptive significance and reflects the modes and intensities of selection mechanisms operating on the virus. In this work, we sought to investigate using classical population genetics parameters, the genetic variability of HCV genotype 1 using the 5' UTR and NS5A regions from treatment non-responding and responding groups of patients. Both regions showed low genetic variability and the 5' UTR showed neutral deviation. No differences were observed in the nonsynonymous/synonymous nucleotide substitution ratio among groups for NS5A. The analysis of molecular variance test of the 5' UTR region showed an 11.94% variation among groups. Phylogenetic analysis showed no correlation between sequence variations and therapeutic responses.
authors.affiliation: Instituto de Pesquisa René Rachou-Fiocruz, Belo Horizonte, MG, Brasil. araujo@cpqrr.fiocruz.br
authors.forename: Flávio M G
authors.initials: FM
authors.lastname: Araújo
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Sonoda
authors.forename: Nilton B
authors.initials: NB
authors.lastname: Rodrigues
authors.forename: Rosangela
authors.initials: R
authors.lastname: Teixeira
authors.forename: Rodrigo A F
authors.initials: RA
authors.lastname: Redondo
authors.forename: Guilherme C
authors.initials: GC
authors.lastname: Oliveira
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D000998:Antiviral Agents / Q000627:therapeutic use; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000188:drug therapy / Q000821:virology*; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000627:therapeutic use; D010802:Phylogeny; D011092:Polyethylene Glycols / Q000627:therapeutic use; D012367:RNA, Viral / Q000235:genetics; D011994:Recombinant Proteins; D012254:Ribavirin / Q000627:therapeutic use; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase
chemical_list: D020121:5' Untranslated Regions; D000998:Antiviral Agents; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011092:Polyethylene Glycols; D012367:RNA, Viral; D011994:Recombinant Proteins; D012254:Ribavirin; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase; C100416:peginterferon alfa-2a