title: Association of insertion/deletion polymorphism of alpha-adrenoceptor gene in essential hypertension with or without type 2 diabetes mellitus in Malaysian subjects.
abstract: An insertion/deletion (I/D) polymorphism of Alpha2B-Adrenoceptor (ADRA2B) gene located on chromosome 2 has been studied extensively in related to cardiovascular diseases. The main aim of the present study was to examine the potential association of D allele frequency of I/D polymorphism of ADRA2B gene in Malaysian essential hypertensive subjects with or without type 2 diabetes mellitus (T2DM). This study includes 70 hypertensive subjects without T2DM, 65 hypertensive subjects with T2DM and 75 healthy volunteers as control subjects. Genotyping of I/D polymorphism was performed by conventional PCR method. There was significant difference found in age, body mass index, systolic/diastolic blood pressure and high density lipoprotein cholesterol level between the case and control subjects. DD genotypic frequency of I/D polymorphism was significantly higher in hypertensive subjects (42.84% vs. 29.33%; P-=0.029) and in hypertensive with T2DM subjects (46.15% vs. 29.33%; P=0.046) than control group. D allele frequency was higher in hypertensive group (67.41%) than control subjects (52.67%). However, no significant difference was found between the three genotypes of I/D polymorphism of ADRA2B gene and the clinical characteristics of the subjects. The result obtained in this study show D allele of ADRA2B gene was associated with essential hypertension with or without T2DM in Malaysian subjects.
authors.affiliation: Molecular Biology Lab, Department of Biomedical Science, Universiti Putra Malaysia, Serdang 43400, Malaysia.
authors.forename: R
authors.initials: R
authors.lastname: Vasudevan
authors.forename: Patimah
authors.initials: P
authors.lastname: Ismail
authors.forename: Johnson
authors.initials: J
authors.lastname: Stanslas
authors.forename: Norashikin
authors.initials: N
authors.lastname: Shamsudin
authors.forename: Aisyah Binti
authors.initials: AB
authors.lastname: Ali
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000150:complications / Q000235:genetics*; D054643:INDEL Mutation; D008296:Malaysia; D008297:Male; D008875:Middle Aged; D018341:Receptors, Adrenergic, alpha-2 / Q000235:genetics*
chemical_list: C547771:ADRA2B protein, human; D018341:Receptors, Adrenergic, alpha-2