title: Structure and function of a transcriptional network activated by the MAPK Hog1.
abstract: Cells regulate gene expression using a complex network of signaling pathways, transcription factors and promoters. To gain insight into the structure and function of these networks, we analyzed gene expression in single- and multiple-mutant strains to build a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast. Our model reveals that the Hog1 and general stress (Msn2/4) pathways interact, at both the signaling and promoter level, to integrate information and create a context-dependent response. This study lays out a path to identifying and characterizing the role of signal integration and processing in other gene regulatory networks.
authors.affiliation: Howard Hughes Medical Institute, Harvard University Faculty of Arts and Sciences Center for Systems Biology, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138, USA.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Capaldi
authors.forename: Tommy
authors.initials: T
authors.lastname: Kaplan
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Naomi
authors.initials: N
authors.lastname: Habib
authors.forename: Aviv
authors.initials: A
authors.lastname: Regev
authors.forename: Nir
authors.initials: N
authors.lastname: Friedman
authors.forename: Erin K
authors.initials: EK
authors.lastname: O'Shea
mesh_terms: D001665:Binding Sites; D047369:Chromatin Immunoprecipitation; D004789:Enzyme Activation; D015966:Gene Expression Regulation, Fungal; D053263:Gene Regulatory Networks; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C088589:HOG1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases