Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform.
A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.
Leblanc
Jason J
JJ
Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada.
Pettipas
Janice
J
Davidson
Ross J
RJ
Tipples
Graham A
GA
Hiebert
Joanne
J
Hatchette
Todd F
TF
D006801:Humans; D009107:Mumps / Q000175:diagnosis* / Q000821:virology; D009109:Mumps virus / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D043262:Ribonuclease P / Q000235:genetics; D012680:Sensitivity and Specificity; D014760:Viral Fusion Proteins / Q000235:genetics
D012367:RNA, Viral; D014760:Viral Fusion Proteins; D043262:Ribonuclease P