title: Purification and characterization of a new aldehyde oxidase from pseudomonas sp. AIU 362.
abstract: An aldehyde oxidase exhibiting high activity on glyoxal was purified to an electrophoretically homogenous state from Pseudomonas sp. AIU 362, which was isolated from a soil sample using a methoxyethanol medium. The enzyme oxidized not only glyoxal but also short-chain aliphatic aldehydes and aromatic aldehydes. Thus, this enzyme was classified into the aldehyde oxidase (ALOD) group. However, it was composed of four identical subunits with a molecular mass of 27 kDa, whereas other microbial ALODs were composed of three hetero subunits, and ALODs from plant and animals were composed of two identical subunits. The NH(2)-terminal sequence also showed no similarity to that of other ALODs. These results indicate that ALOD from Pseudomonas sp. AIU 362 is a new aldehyde oxidase. This ALOD was induced by 2-methoxyethanol, methanol or isopropanol.
authors.affiliation: The United Graduate School of Agricultural Sciences, Iwate University, 18-8 Ueda 3- chome, Morioka 020-8550, Japan.
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Kimiyasu
authors.initials: K
authors.lastname: Isobe
authors.forename: Michihiko
authors.initials: M
authors.lastname: Kataoka
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.forename: Akira
authors.initials: A
authors.lastname: Iwasaki
authors.forename: Junzo
authors.initials: J
authors.lastname: Hasegawa
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D042931:Aldehyde Oxidase / Q000737:chemistry* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D004789:Enzyme Activation; D004795:Enzyme Stability; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011549:Pseudomonas / Q000145:classification* / Q000201:enzymology*; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D042931:Aldehyde Oxidase