title: Association of single nucleotide polymorphisms in the IL-18 gene with production of IL-18 protein by mononuclear cells from healthy donors.
abstract: IL-18 has proinflammatory effects and participates in both innate and adaptive cellular and humoral immunity. A number of SNPs that influence IL-18 production are found in the gene promoter region. We investigated the association of SNPs in the IL-18 promoter at -607 and -137 with the level of IL-18 protein production by PBMC from healthy donors from Southwestern Siberia. The genetic distribution of these SNPs in the promoter site was established by PCR. IL-18 protein production was determined by ELISA. Our results showed that PBMC from donors carrying allele 137C have lower levels of both spontaneous and LPS-stimulated IL-18 production. In contrast, PBMC from donors carrying allele 607A showed significant increases in spontaneous and stimulated IL-18 production compared to wild type. Our study suggests that the SNPs -607 and -137 in the promoter region of the IL-18 gene influence the level of IL-18 protein production by PBMC from healthy donors in Southwestern Siberia.
authors.affiliation: Laboratory of Molecular Immunology, Institute of Clinical Immunology , Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630099, Russia.
authors.forename: Olga Pavlovna
authors.initials: OP
authors.lastname: Khripko
authors.forename: Nadegda Sergeevna
authors.initials: NS
authors.lastname: Sennikova
authors.forename: Julia Anatolievna
authors.initials: JA
authors.lastname: Lopatnikova
authors.forename: Jurii Ivanovich
authors.initials: JI
authors.lastname: Khripko
authors.forename: Maxim Leonidovich
authors.initials: ML
authors.lastname: Filipenko
authors.forename: Evgenii Alexandrovich
authors.initials: EA
authors.lastname: Khrapov
authors.forename: Evgenii L'vovich
authors.initials: EL
authors.lastname: Gelfgat
authors.forename: Elena Vladimirovna
authors.initials: EV
authors.lastname: Yakushenko
authors.forename: Vladimir Alexandrovich
authors.initials: VA
authors.lastname: Kozlov
authors.forename: Sergei Vitalievich
authors.initials: SV
authors.lastname: Sennikov
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D005838:Genotype; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012800:Siberia; D055815:Young Adult
chemical_list: D020382:Interleukin-18