title: [Expression variation and significance of Skp2 and p27(kip1) during the proliferation of Jurkat cells].
abstract: OBJECTIVE
  To investigate the expression variation and significance of Skp2 and p27(kip1) during the proliferation of lymphoma cell line Jurkat cells.
  
  
  METHODS
  The binding of p27(kip1) and Skp2 in Jurkat cells were detected by immunoprecipitation. Jurkat cells were treated with serum starvation and release synchronization. The expression variation and subcellular localization of p27(kip1) and Skp2 were detected by subcellular fractionation, Western blot and double immunofluorescence labelling.
  
  
  RESULTS
  The results of immunoprecipitation suggested that p27(kip1) and Skp2 could bind each other in Jurkat cells. During the proliferation of Jurkat cells, the protein expression of p27(kip1) decreased and intranuclear p27(kip1) decreased significantly, while the Skp2 protein increased and cytoplasmic Skp2 increased significantly.
  
  
  CONCLUSIONS
  During the proliferation of Jurkat cells, the increased cytoplasmic synthesis of Skp2 may speed up p27(kip1) degradation via the ubiquitin-proteasome pathway, then intranuclear p27(kip1) decreases significantly, leading to an increased cell cycling activity.
authors.affiliation: Department of Microbiology and Immunology, Nantong University, Nantong 226001, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Lu
authors.forename: Yu-Chan
authors.initials: YC
authors.lastname: Wang
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Shen
authors.forename: Yue-Ming
authors.initials: YM
authors.lastname: Zhao
authors.forename: Cheng-Long
authors.initials: CL
authors.lastname: Sun
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Zhang
authors.forename: Chun
authors.initials: C
authors.lastname: Cheng
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D016393:Lymphoma, B-Cell / Q000378:metabolism / Q000473:pathology; D011485:Protein Binding; D044786:S-Phase Kinase-Associated Proteins / Q000378:metabolism*
chemical_list: D044786:S-Phase Kinase-Associated Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27