[Antitumor activities against hepatocellular carcinoma induced by bone marrow mesenchymal stem cells pulsed with tumor-derived exosomes].
OBJECTIVE
To evaluate the antitumor activities against hepatocellular carcinoma induced by bone marrow mesenchymal stem cells(MSCs) from BALB/c mice pulsed with tumor-derived exosomes.


METHODS
Exosomes derived from malignant ascites of hepatocellular carcinoma were isolated and purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation from BALB/c mice. Bone marrow MSCs from BALB/c mice were obtained using adherent culture method combining with magnetic cell sorting (MACS). IFN-gamma stimulated MSCs were pulsed with tumor-derived exosomes, Then the stimulated MSCs cocultured with H22 hepatocellular carcinoma for 72 hours, the proliferation of H22 hepatocellular carcinoma cells were evaluated.


RESULTS
The proliferation of H22 hepatocellular carcinoma cells cocultured with MSCs plused with tumor-derived exosomes, were significantly inhibited (P < 0.05).


CONCLUSIONS
When bone marrow MSCs were pulsed with tumor-derived exosomes, MSCs showed enhanced antitumor activities against hepatocellular carcinoma. Therefore, MSCs combined with TEX may offer an effective method to induce antitumor activities.
Ma
Bo
B
Department of Medical Oncology, Beijing Cancer Hospital & Institute, Peking University School of Oncology, Beijing 100142, China.
Ren
Jun
J
Jiang
Han-fang
HF
Jia
Jun
J
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D018920:Coculture Techniques; D055354:Exosomes / Q000276:immunology*; D005260:Female; D007167:Immunotherapy; D008114:Liver Neoplasms, Experimental / Q000276:immunology / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured