title: Beta amyloid and hyperphosphorylated tau deposits in the pancreas in type 2 diabetes.
abstract: Strong epidemiologic evidence suggests an association between Alzheimer disease (AD) and type 2 diabetes. To determine if amyloid beta (Abeta) and hyperphosphorylated tau occurs in type 2 diabetes, pancreas tissues from 21 autopsy cases (10 type 2 diabetes and 11 controls) were analyzed. APP and tau mRNAs were identified in human pancreas and in cultured insulinoma beta cells (INS-1) by RT-PCR. Prominent APP and tau bands were detected by Western blotting in pancreatic extracts. Aggregated Abeta, hyperphosphorylated tau, ubiquitin, apolipoprotein E, apolipoprotein(a), IB1/JIP-1 and JNK1 were detected in Langerhans islets in type 2 diabetic patients. Abeta was co-localized with amylin in islet amyloid deposits. In situ beta sheet formation of islet amyloid deposits was shown by infrared microspectroscopy (SIRMS). LPS increased APP in non-neuronal cells as well. We conclude that Abeta deposits and hyperphosphorylated tau are also associated with type 2 diabetes, highlighting common pathogenetic features in neurodegenerative disorders, including AD and type 2 diabetes and suggesting that Abeta deposits and hyperphosphorylated tau may also occur in other organs than the brain.
authors.affiliation: Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, BC, Canada. judithmiklossy@bluewin.ch
authors.forename: Judith
authors.initials: J
authors.lastname: Miklossy
authors.forename: Hong
authors.initials: H
authors.lastname: Qing
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Radenovic
authors.forename: Andras
authors.initials: A
authors.lastname: Kis
authors.forename: Bertrand
authors.initials: B
authors.lastname: Vileno
authors.forename: Forró
authors.initials: F
authors.lastname: Làszló
authors.forename: Lisa
authors.initials: L
authors.lastname: Miller
authors.forename: Ralph N
authors.initials: RN
authors.lastname: Martins
authors.forename: Gerard
authors.initials: G
authors.lastname: Waeber
authors.forename: Vincent
authors.initials: V
authors.lastname: Mooser
authors.forename: Fred
authors.initials: F
authors.lastname: Bosman
authors.forename: Kamel
authors.initials: K
authors.lastname: Khalili
authors.forename: Nune
authors.initials: N
authors.lastname: Darbinian
authors.forename: Patrick L
authors.initials: PL
authors.lastname: McGeer
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D010766:Phosphorylation; D014018:Tissue Distribution; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D016875:tau Proteins