title: Simultaneous estimation of null alleles and inbreeding coefficients.
abstract: Although microsatellites are a very efficient tool for many population genetics applications, they may occasionally produce "null" alleles, which, when present in high proportion, may affect estimates of key parameters such as inbreeding and relatedness coefficients or measures of genetic differentiation. In order to account for the presence of null alleles, it is first necessary to estimate their frequency within studied populations. However, the commonly used null allele frequency estimators are not of general applicability because they can produce upwardly biased estimates when a population under study experiences some inbreeding. In such a case, 2 formerly described approaches, population inbreeding model and individual inbreeding model, can be applied for simultaneous estimation of null allele frequencies and of the inbreeding coefficient. In this study, we demonstrate the properties and utility of these 2 methods and show that they outperform the commonly used approaches in the estimation of null allele frequencies based on genotypic data. The methods are applied to empirical data from a natural population of European beech (Fagus sylvatica L.), and results are briefly discussed. The methods presented in this paper are implemented in the Windows-based user-friendly INEST computer program (available free of charge at http://genetyka.ukw.edu.pl/INEst10_setup.exe).
authors.affiliation: the Department of Genetics, Institute of Experimental Biology, Kazimierz Wielki University, Bydgoszcz 85-064, Poland. igorchy@ukw.edu.pl
authors.forename: Igor J
authors.initials: IJ
authors.lastname: Chybicki
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Burczyk
mesh_terms: D000483:Alleles; D003198:Computer Simulation; D029964:Fagus / Q000235:genetics; D005787:Gene Frequency; D005828:Genetics, Population; D007178:Inbreeding; D018895:Microsatellite Repeats / Q000235:genetics