RNA bandages for photoregulating in vitro protein synthesis.
'RNA bandages' are composed of two 6-12-mer 2'-OMe RNA strands complementary to a mRNA target that are joined by a photocleavable linker. These tandem oligonucleotides typically exhibit much higher affinity for the mRNA than the individual strands. An RNA bandage with binding arms of different lengths and a 4-base gap blocked translation in vitro of GFP mRNA; subsequent near-UV irradiation restored translation. This provides a general method of photomodulating hybridization for a variety of oligonucleotide-based technologies.
Richards
Julia L
JL
Department of Chemistry, University of Pennsylvania, 231 South 34th St., Philadelphia, PA 19104-6323, USA.
Tang
XinJing
X
Turetsky
Anna
A
Dmochowski
Ivan J
IJ
D009693:Nucleic Acid Hybridization / Q000528:radiation effects; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry / Q000528:radiation effects*; D014176:Protein Biosynthesis / Q000187:drug effects; D012313:RNA / Q000737:chemistry* / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics; D012270:Ribosomes / Q000187:drug effects; D012689:Sequence Homology, Nucleic Acid
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D012313:RNA