title: [HBV infection downregulated Mre11 expression and induced genome instability].
abstract: OBJECTIVE
  Prolonged infection with Hepatitis B virus (HBV) has been recognized as a major factor for hepatocellular carcinoma (HCC). In this study, we studied the host protein Mrel11 fluctuations after HBV infection which might be eventually contributed to cell transformation.
  
  
  METHODS
  Western Blot was monitored to detect the expression of Mre11, and RNA interference was used to downregulate protein expression. Then, Ligation mediated PCR was used to detect the level of DNA double strand breaks.
  
  
  RESULTS
  Mre11 protein was downregulated when HBV infection occured, and the downregulated expression was also seen in HCC tissue. By RNA interference, we found that Mre11 knockdown caused DNA instability.
  
  
  CONCLUSIONS
  Mre11 expression downregulation contributed at least partially to cell transformation caused by HBV infection.
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100850, China. liuyu08@yahoo.com
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Ningbo
authors.initials: N
authors.lastname: Hou
authors.forename: Fan
authors.initials: F
authors.lastname: Zhao
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.forename: Hui
authors.initials: H
authors.lastname: Zhong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D042822:Genomic Instability; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D000076228:MRE11 Homologue Protein; D034622:RNA Interference
chemical_list: D004268:DNA-Binding Proteins; C121766:MRE11 protein, human; D000076228:MRE11 Homologue Protein