title: Cloning and expression of arylalkylamine N-acetyltranferase-2 during early development and metamorphosis in the sole Solea senegalensis.
abstract: The arylalkylamine N-acetyltransferase (AANAT) is a key enzyme in the rhythmic production of melatonin. Two Aanats are expressed in teleost fish, one retinal specific, Aanat1, and the other one pineal specific, Aanat2, being the latter the main enzyme responsible of the plasma nocturnal melatonin increase in fish. In anurans melatonin has been involved in metamorphosis through antagonizing thyroid hormone function; however, no available data reports a relationship between melatonin system and metamorphosis in fish. In this study, we have cloned the AANAT2 (SsAanat2) in a flatfish, Solea senegalensis, and studied its sites of expression and developmental expression pattern by in situ hybridization and Real Time PCR. These studies allowed us to demonstrate a specific signal in the pineal gland of sole larvae from 2 days post-fertilization (dpf), which was evident until post-metamorphosis. Immunohistochemical analysis on the hybridized slides showed that the sole pineal Aanat2 expressing cells corresponded to pineal photoreceptor cells. Real Time PCR was performed in animals kept under natural photoperiod and sampled at different stages from 0 to 21 dpf (including pre-, early-, middle- and late-metamorphic stages) and at midlight (ML) and middark (MD) daytimes. Sole Aanat2 expression was higher at MD than at ML from 2 dpf and at most developmental stages analyzed. The highest AANAT2 mRNA abundance was observed at 2 and 4 dpf. A significant 60-fold reduction in Aanat2 expression was seen just before metamorphosis demonstrating, for the first time in a vertebrate species, that the expression of pineal AANAT and thyroid hormones levels exhibit an inverse pattern during metamorphosis.
authors.affiliation: Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, E-11510, Puerto Real, España, Spain. eisornaa@bio.ucm.es
authors.forename: Esther
authors.initials: E
authors.lastname: Isorna
authors.forename: Abdeslam
authors.initials: A
authors.lastname: El M'rabet
authors.forename: Francesca
authors.initials: F
authors.lastname: Confente
authors.forename: Jack
authors.initials: J
authors.lastname: Falcón
authors.forename: José A
authors.initials: JA
authors.lastname: Muñoz-Cueto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D047068:Arylalkylamine N-Acetyltransferase / Q000235:genetics*; D001483:Base Sequence; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D015536:Down-Regulation; D005412:Flatfishes / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D007814:Larva / Q000201:enzymology; D008675:Metamorphosis, Biological / Q000502:physiology*; D008969:Molecular Sequence Data; D020419:Photoreceptor Cells, Vertebrate / Q000201:enzymology; D010802:Phylogeny; D010870:Pineal Gland / Q000201:enzymology; D016133:Polymerase Chain Reaction
chemical_list: D047068:Arylalkylamine N-Acetyltransferase