In situ temperature-jump dynamic nuclear polarization: enhanced sensitivity in two dimensional 13C-13C correlation spectroscopy in solution.
Dynamic nuclear polarization is combined with temperature-jump methods to develop a new 2D 13C-13C NMR experiment that yields a factor of 100-170 increase in sensitivity. The polaization step is performed at 100 K, and the sample is subsequently melted with a 10.6 microm laser pulse to yield a sample with highly polarized 13C spins. 13C detected 2D 13C-13C spectroscopy is performed in the usual manner.
Joo
Chan-Gyu
CG
Francis Bitter Magnet Laboratory and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Casey
Andrew
A
Turner
Christopher J
CJ
Griffin
Robert G
RG
D002247:Carbon Isotopes; D017666:Deuterium Oxide / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012680:Sensitivity and Specificity; D012996:Solutions; D013696:Temperature; D014867:Water / Q000737:chemistry
D002247:Carbon Isotopes; D012996:Solutions; D014867:Water; D005947:Glucose; D017666:Deuterium Oxide