title: Expression, purification, crystallization and preliminary X-ray diffraction studies of glyceraldehyde-3-phosphate dehydrogenase 1 from methicillin-resistant Staphylococcus aureus (MRSA252).
abstract: Glyceraldehyde-3-phosphate dehydrogenase 1 from methicillin-resistant Staphylococcus aureus (MRSA252) was cloned in pQE30 vector, overexpressed in Escherichia coli M15(pREP4) cells and purified to homogeneity. The protein was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group P2(1), with unit-cell parameters a = 65.23, b = 95.58, c = 87.91 A, beta = 106.5 degrees . X-ray diffraction data were collected and processed to a maximum resolution of 2.0 A. The presence of one tetramer in the asymmetric unit gave a Matthews coefficient (V(M)) of 1.78 A(3) Da(-1) and a solvent content of 31%. The structure was solved by molecular replacement and structure refinement is now in progress.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur 721 302, India.
authors.forename: Somnath
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Debajyoti
authors.initials: D
authors.lastname: Dutta
authors.forename: Baisakhee
authors.initials: B
authors.lastname: Saha
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Das
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016106:Methicillin Resistance; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000201:enzymology*; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D001426:Bacterial Proteins; C055686:glyceraldehyde 3-phosphate phosphatase; D010744:Phosphoric Monoester Hydrolases