Platelet activating factor (PAF) and activity of its biosynthetic and catabolic enzymes in blood and leukocytes of male patients with newly diagnosed heart failure.
OBJECTIVE
To evaluate platelet activating factor (PAF) levels, its metabolic enzymes activity and its associations with other inflammatory markers in heart failure (HF) patients.


METHODS
PAF, and two of its key biosynthetic enzymes [lyso-PAF acetyltransferase (lyso-PAF-AT) and DTT-insensitive CDP-choline:1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase (PAF-CPT)] along with its catabolic enzymes [PAF-acetylhydrolase (PAF-AH) and lipoprotein-associated phospholipase-A(2) (Lp-PLA(2))] were measured in serum and leukocytes of twelve newly diagnosed male HF patients. Serum CRP, TNF-alpha, IL-6, sCD14 and CD40L were also determined.


RESULTS
PAF ranged from 0.03 to 5.6 pmol/mL. Median lyso-PAF-AT, PAF-CPT, PAF-AH and Lp-PLA(2) activities were 4.1, 68.42, 644.44 pmol/min/mg protein and 51.42 pmol/min/microL correspondingly. Lyso-PAF-AT and PAF-CPT activities positively correlated with CRP, IL-6 and with each other, whereas PAF-CPT activity correlated with sCD14 and CD40L (P<0.05).


CONCLUSIONS
PAF's biosynthetic enzyme activities correlated with inflammatory and immunologic molecules, which are activated in HF. Our study indicates a potential role of PAF in HF patients.
Detopoulou
Paraskevi
P
Department of Nutrition Science-Dietetics, Harokopio University, 70 E1 Venizelou Street, Athens, 17671, Greece.
Nomikos
Tzortzis
T
Fragopoulou
Elisabeth
E
Antonopoulou
Smaragdi
S
Kotroyiannis
Iason
I
Vassiliadou
Carmen
C
Panagiotakos
Demosthenes B
DB
Chrysohoou
Christina
C
Pitsavos
Christos
C
Stefanadis
Christodoulos
C
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000097:blood*; D000123:Acetyltransferases / Q000097:blood*; D000368:Aged; D002798:Diacylglycerol Cholinephosphotransferase / Q000097:blood*; D006333:Heart Failure / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010972:Platelet Activating Factor / Q000378:metabolism*
D010972:Platelet Activating Factor; D000123:Acetyltransferases; C046035:1-alkylglycerophosphocholine acetyltransferase; D002798:Diacylglycerol Cholinephosphotransferase; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase