Identifying protein interactions with metal-modified DNA using microarray technology.
Protein microarrays have been used extensively to identify protein-protein interactions; however, this technology has not been widely applied to protein-DNA interactions. In particular, this work demonstrates the utility of this technique for rapidly identifying interactions of proteins with metal-modified DNA. Protein macroarray experiments were carried out with high mobility group protein 1 (HMG-1) and cisplatin- and chromium-modified 50-mer oligonucleotides to demonstrate "proof of principle." Commercially available protein microarrays containing many different classes of human proteins were then employed to search for additional interactions with cisplatin-modified DNA. The results of the microarray experiments confirmed some known interactions and, more importantly, identified many novel protein interactions, demonstrating the utility of this method as a rapid, high-throughput technique to discover proteins that interact with metal-modified DNA.
Stansfield
Hope E
HE
Department of Chemistry, Biochemistry Program, Smith College, Northampton, MA 01063, USA.
Kulczewski
Bethany P
BP
Lybrand
Kyle E
KE
Jamieson
Elizabeth R
ER
D002857:Chromium / Q000737:chemistry*; D002945:Cisplatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D024243:HMGB1 Protein / Q000378:metabolism*; D006801:Humans; D040081:Protein Array Analysis
D024243:HMGB1 Protein; D002857:Chromium; D004247:DNA; D002945:Cisplatin