title: [Differentiation of bone marrow derived from mesenchymal stem cells into cardiomyocyte-like cells induced by co-culture with rat myocardial cells].
abstract: The study was purposed to investigate the differentiation ability of mesenchymal stem cells (MSCs) into myocardial cells in vitro. Rat bone marrow-derived MSCs were labeled and co-cultured with neonatal rat cardiomyocytes (CM) for 5 - 7 days. The expression of cell surface antigens was detected by flow cytometry, and the expression of muscle-specific marker myosin and troponin T in labeled cells was detected by immunofluorescence. The results showed that in vitro cultured MSCs expressed CD90, CD44, CD105, CD54, not expressed CD34, CD45, CD31. After co-cultured with neonatal rat CM, labeled MSCs differentiated into cardiomyocyte-like cells expressing myosin and troponin T. It is concluded that MSCs can differentiate into cardiomyocyte-like cells when co-cultured with neonatal myocardial cells in vitro. In co-culture of two kind of cells in ratio of four to one showed obvious efficacy differentiating MSCs into CMs.
authors.affiliation: Chinese Academy of Medical Sciences, China.
authors.forename: Rong-Li
authors.initials: RL
authors.lastname: Zhang
authors.forename: Er-Lie
authors.initials: EL
authors.lastname: Jiang
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Zhai
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Zhai
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wang
authors.forename: Yu-Shi
authors.initials: YS
authors.lastname: Bao
authors.forename: Hong
authors.initials: H
authors.lastname: DU
authors.forename: Ming-Zhe
authors.initials: MZ
authors.lastname: Han
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar