Genetic screening of lactic acid bacteria of oenological origin for bacteriocin-encoding genes.
A total of 330 lactic acid bacteria isolated from South African red wines during alcoholic and malolactic fermentations and 9 commercial malolactic bacteria starter cultures were screened for antimicrobial activity. Of the entire screened isolates, 26 strains, belonging to the species Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus hilgardii and Oenococcus oeni, showed activity towards various wine-related and non-wine-related indicator strains. A PCR-based screening revealed the presence of the plantaricin encoding genes plnA, plnEF, plnJ and plnK in five selected Lb. plantarum strains. Furthermore, a co-culture experiment with Lb. plantarum and Enterococcus faecalis was performed. A complete inhibition of cell growth of Ent. faecalis was observed within 72 h. Four putative bacteriocin-encoding genes in the genome of O. oeni were identified and sequenced.
Knoll
Caroline
C
Institute for Wine Biotechnology, Stellenbosch University, Private Bag X1, 7602 Matieland, South Africa.
Divol
Benoit
B
du Toit
Maret
M
D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification*; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D005285:Fermentation; D005516:Food Microbiology; D007778:Lactobacillus / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D013019:South Africa; D013045:Species Specificity; D014920:Wine / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004269:DNA, Bacterial