Sex differentiation and vitellogenin and 11-ketotestosterone levels in chub, Leuciscus cephalus L., exposed to 17 beta-estradiol and testosterone during early development.
The effects of 17 beta-estradiol (E(2)) and testosterone (T) singly and in combination were tested on juvenile chub (Leuciscus cephalus L.). Vitellogenin (VTG) and 11-ketotestosterone (11-KT) were determined by ELISA in whole body homogenates and the gonads were examined histologically. Testosterone and estradiol, in combination, significantly increased whole body VTG (p < 0.01), but not 11-KT, compared to controls and the T treated groups. The only intersex observed (1/80) was in the combined treatment group. We suggest that VTG measured in whole body homogenates could be used to determine the effects of exogenous steroids in juvenile chub.
Zlábek
Vladimír
V
Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceské Budejovice, Vodnany, Czech Republic. zlabek@vurh.jcu.cz
Randák
T
T
Kolárová
J
J
Svobodová
Z
Z
Kroupová
H
H
D000818:Animals; D003530:Cyprinidae / Q000254:growth & development / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000633:toxicity*; D005260:Female; D008297:Male; D012733:Sex Differentiation / Q000187:drug effects*; D012744:Sex Ratio; D013739:Testosterone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity*; D014819:Vitellogenins / Q000378:metabolism*
D052244:Endocrine Disruptors; D014819:Vitellogenins; D013739:Testosterone; D004958:Estradiol; C003600:11-ketotestosterone