title: pH-sensitive genipin-cross-linked chitosan microspheres for heparin removal.
abstract: Chitosan hydrogel microspheres were obtained by cross-linking chitosan in its inverse emulsion using genipin as cross-linker. The genipin-cross-linked chitosan microspheres (ChGp) swell significantly in water at pH values below 6.5 and shrink to a smaller extent at pH values above 6.5. ChGp microspheres bind heparin in water. The kinetics of heparin binding was found to be pH dependent and was faster and more efficient at a lower pH. That can be also controlled by the weight of ChGp microspheres used. Rate and efficiency of heparin adsorption at pH 7.4, which is typical of blood, could be increased by quaternization of ChGp microspheres using glycidyltrimethylammonium chloride (GTMAC). The polymeric material obtained thus can be potentially useful for heparin removal in biomedical applications.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Kamiński
authors.forename: Karolina
authors.initials: K
authors.lastname: Zazakowny
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szczubiałka
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
mesh_terms: D000327:Adsorption; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents; D006493:Heparin / Q000493:pharmacokinetics*; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057889:Iridoid Glycosides; D039823:Iridoids / Q000737:chemistry*; D007700:Kinetics; D008863:Microspheres
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D057889:Iridoid Glycosides; D039823:Iridoids; D006493:Heparin; D048271:Chitosan; C007834:genipin