Characterization of a Bacillus subtilis 64-kDa DNA polymerase X potentially involved in DNA repair.
Bacillus subtilis gene yshC encodes a 64-kDa family X DNA polymerase (PolXBs), which contains all the critical residues involved in DNA and nucleotide binding as well as those responsible for catalysis of DNA polymerization, conserved in most family X members. Biochemical analyses of the purified enzyme indicate that PolXBs is a monomeric and strictly template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to a few nucleotides and bearing a phosphate group at the 5' end of the downstream DNA. The fact that PolXBs is able to conduct filling of a single-nucleotide gap, allowing further sealing of the resulting nick by a DNA ligase, points to a putative role in base excision repair during the B. subtilis life cycle.
Baños
Benito
B
Instituto de Biología Molecular Eladio Viñuela (CSIC), Centro de Biología Molecular Severo Ochoa (CSIC-UAM), C/Nicolás Cabrera 1, Cantoblanco, 28049 Madrid, Spain.
Lázaro
José M
JM
Villar
Laurentino
L
Salas
Margarita
M
de Vega
Miguel
M
D000595:Amino Acid Sequence; D001105:Archaea / Q000201:enzymology; D001412:Bacillus subtilis / Q000201:enzymology*; D019951:DNA Polymerase beta / Q000737:chemistry; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009711:Nucleotides; D016415:Sequence Alignment; D013379:Substrate Specificity
D004269:DNA, Bacterial; D009711:Nucleotides; C109534:DNA polymerase X; D019951:DNA Polymerase beta; D004259:DNA-Directed DNA Polymerase