title: Antigenic and genetic analysis of H5 influenza viruses isolated from water birds for the purpose of vaccine use.
abstract: In order to prepare H5N1 influenza virus vaccine, the hemagglutinins (HAs) of 14 H5 virus isolates from water birds in Asia were antigenically and genetically analyzed. Phylogenetic analysis of the H5 HA genes revealed that 13 isolates belong to Eurasian and the other one to North American lineages. Each of the deduced amino acid sequences of the HAs indicated a non-pathogenic profile. Antigenic analysis using a panel of monoclonal antibodies recognizing six different epitopes on the HA of A/duck/Pennsylvania/10218/1984 (H5N2) and chicken antiserum to an H5N1 reassortant strain generated between A/duck/Mongolia/54/2001 (H5N2) and A/duck/Mongolia/47/2001 (H7N1), [R(Dk/Mong-Dk/Mong) (H5N1)] showed that the HAs of highly pathogenic avian influenza (HPAI) viruses currently circulating in Asia were antigenically closely related to those of the present isolates from water birds. Mice subcutaneously injected with formalin-inactivated R(Dk/Mong-Dk/Mong) were protected from challenge with 100 mouse lethal dose of A/Viet Nam/1194/2004 (H5N1). The present results support the notion that the H5 isolates and the reassortant H5N1 strain should be useful for vaccine preparation.
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, Sapporo, Hokkaido, 060-0818, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Soda
authors.forename: Hiroichi
authors.initials: H
authors.lastname: Ozaki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.forename: Norikazu
authors.initials: N
authors.lastname: Isoda
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Haraguchi
authors.forename: Saori
authors.initials: S
authors.lastname: Sakabe
authors.forename: Noritaka
authors.initials: N
authors.lastname: Kuboki
authors.forename: Noriko
authors.initials: N
authors.lastname: Kishida
authors.forename: Ayato
authors.initials: A
authors.lastname: Takada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology*; D000956:Antigens, Viral / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D001208:Asia; D001717:Birds / Q000821:virology*; D002642:Chick Embryo; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines