title: Validation of a method for the determination of sterols and triterpenes in the aerial part of Justicia anselliana (Nees) T. Anders by capillary gas chromatography.
abstract: An accurate and sensitive method, combining soxhlet extraction, solid phase-extraction and capillary gas chromatography is described for the quantitative determination of one triterpene (lupeol) and three sterols (stigmasterol, campesterol and beta-sitosterol) and the detection of another triterpene (alpha-amyrin) from the aerial part of Justicia anselliana. This is the first method allowing the quantification of sterols and triterpenes in this plant. It has been fully validated in order to be able to compare the sterol and triterpene composition of different samples of J. anselliana and therefore help to explain the allelopathic activity due to these compounds. This method showed that the aerial part of J. anselliana contained (292+/-2)mg/kg of lupeol, (206+/-1)mg/kg of stigmasterol, (266+/-2)mg/kg of campesterol and (184+/-9)mg/kg of beta-sitosterol.
authors.affiliation: Pharmacognosy and Essential Oils Laboratory, Faculty of Health Science (FSS), Faculty of Sciences and Technics (FAST), University of d'Abomey-Calavi (UAC), 01 BP 188 Cotonou, Benin. 30-CHAM, Av Mounier 72, B-1200 Bruxelles, Belgium.
authors.forename: Dossou Sika Salomé
authors.initials: DS
authors.lastname: Kpoviéssi
authors.forename: Fernand
authors.initials: F
authors.lastname: Gbaguidi
authors.forename: Joachim
authors.initials: J
authors.lastname: Gbénou
authors.forename: Georges
authors.initials: G
authors.lastname: Accrombessi
authors.forename: Mansourou
authors.initials: M
authors.lastname: Moudachirou
authors.forename: Eric
authors.initials: E
authors.lastname: Rozet
authors.forename: Philippe
authors.initials: P
authors.lastname: Hubert
authors.forename: Joëlle
authors.initials: J
authors.lastname: Quetin-Leclercq
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D002138:Calibration; D002784:Cholesterol / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes; D010840:Phytosterols / Q000032:analysis / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012855:Sitosterols / Q000032:analysis / Q000737:chemistry; D052616:Solid Phase Extraction; D013261:Sterols / Q000032:analysis* / Q000737:chemistry; D013265:Stigmasterol / Q000032:analysis / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry; D000094042:Lupanes
chemical_list: D002784:Cholesterol; D053978:Pentacyclic Triterpenes; D010840:Phytosterols; D012855:Sitosterols; D013261:Sterols; D013265:Stigmasterol; D014315:Triterpenes; C021273:campesterol; C025473:gamma-sitosterol; C010480:lupeol; D000094042:Lupanes