title: Efficacy of a new ochratoxin-binding agent (OcraTox) to counteract the deleterious effects of ochratoxin A in laying hens.
abstract: An experiment was conducted to evaluate the efficacy of a new ochratoxin-binding agent (Ocra-Tox, 5 g/kg of feed) in offsetting the toxic effects of ochratoxin A (OTA, 2 mg/kg of feed) in laying hen diets. Performance, serum biochemistry, OTA residue in the liver and eggs, and egg quality parameters were evaluated. Twenty-eight Hisex Brown laying hens, 47 wk of age, were allocated to 1 of 4 experimental treatments for 3 wk: control, OTA (containing 2 mg of OTA/kg of feed), OcraTox (containing 5 g of OcraTox/kg of feed), and OTA + OcraTox (containing 2 mg of OTA and 5 g of OcraTox/kg of feed). Laying hens fed OcraTox showed results similar to the control hens (P > 0.05). The OTA diet significantly (P < 0.05) reduced daily feed consumption, egg mass production, and serum triglyceride concentrations, and increased the relative liver weight, the serum activity of alkaline phosphatase, and the serum concentration of uric acid as compared with the control diet. Addition of OcraTox to the contaminated diet alleviated (P < 0.05) the negative effects resulting from OTA, reaching values not significantly different from the control diet for most of the parameters except the relative weight of the liver. Birds fed the OTA treatment showed a greater content of OTA in the liver (15.1 microg/kg) than those fed the control diet (<0.05 microg/kg). Supplementing the contaminated diet with OcraTox (OTA + OcraTox) reduced the values to 12.0 microg/kg. Residues of OTA were not detected above our detection limit (0.05 microg/kg) in any of the analyzed eggs. In conclusion, our results indicated that addition of OcraTox can counteract the deleterious effects caused by OTA in laying hens.
authors.affiliation: Grup de Recerca en Nutrició, Maneig i Benestar Animal, Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Denli
authors.forename: J C
authors.initials: JC
authors.lastname: Blandon
authors.forename: M E
authors.initials: ME
authors.lastname: Guynot
authors.forename: S
authors.initials: S
authors.lastname: Salado
authors.forename: J F
authors.initials: JF
authors.lastname: Perez
mesh_terms: D000821:Animal Feed; D000818:Animals; D002645:Chickens; D004350:Drug Residues / Q000032:analysis; D004528:Egg Shell / Q000033:anatomy & histology / Q000187:drug effects; D004531:Eggs / Q000032:analysis*; D005260:Female; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D009183:Mycotoxins / Q000037:antagonists & inhibitors; D009793:Ochratoxins / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D010058:Oviposition / Q000187:drug effects*; D010063:Ovum / Q000187:drug effects / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
chemical_list: D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A