[Improvement of the silver-stained technique of polyacrylamide gel electrophoresis.].
Polyacrylamide gel electrophoresis is widely applied in the SSR, SNP markers analysis and the construction of genetic map. It is disadvantage to use conventional silver-stained methods in experiment due to taking a very long time and having fussy steps. In this paper, a silver-stained method of DNA in PAGE gel was improved. The improved method is rapid banding which only takes about 20 min, and the high definition picture was obtained.
Liang
Hong-Wei
HW
1. Key Laboratory of Fish Germplasm Resources and Biotechnology Certificated by Ministry of Agriculture,Yangtze River Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China; 2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; E-mail:lianghw@yfi.ac.cn.
Wang
Chang-Zhong
CZ
Li
Zhong
Z
Luo
Xiang-Zhong
XZ
Zou
Gui-Wei
GW
D004247:DNA; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D012834:Silver; D016622:Silver Staining; D013194:Staining and Labeling
D012834:Silver; D004247:DNA