[Construction and identification of small interfering RNA expression plasmid targeting Sox9 and the function to cell growth and apoptosis of human chondrosarcoma cells HTB94].
OBJECTIVE
To construct small interfering (siRNA) Sox9 expression plasmid and transfer it into human chondrosarcoma cells HTB-94, and to check the mRNA and protein expression of Sox9 and cell growth and apoptosis of HTB-94 human chondrosarcoma cells.


METHODS
siRNA(Sox9) expression plasmid was designed and synthesized. And it was transferred into HTB-94 human chondrosarcoma cells. Then the expression of the mRNA and protein of Sox9, cell growth and apoptosis in transferred HTB-94 human chondrosarcoma cells were checked.


RESULTS
The recombinant plasmid was confirmed by enzyme digestion analysis and DNA sequencing. The expression of the mRNA and protein expression of Sox9 in transferred HTB-94 were significantly reduced. The cell growth of HTB-94 was inhibited, and the apoptosis of HTB-94 was remarkably increased.


CONCLUSIONS
siRNA (Sox9) expression plasmid could be transferred into HTB-94 human chondrosarcoma cells. And it can reduce the mRNA and protein expression of the HTB-94, inhibit the cell growth and cause the apoptosis of the tumor cells.
Qin
Hong-Min
HM
Xuzhou Medical College Hospital, Xuzhou 221002, China. qinhongmin07@yahoo.com.cn
Han
Hui-Feng
HF
Xu
Tie
T
Sha
Guang-Zhao
GZ
Liu
Lin
L
Peng
Yi-Gen
YG
Ren
Tian-Cheng
TC
D017209:Apoptosis; D049109:Cell Proliferation; D002813:Chondrosarcoma / Q000378:metabolism / Q000473:pathology*; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D055758:SOX9 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D055758:SOX9 Transcription Factor