Effects of copper ions released from metallic copper on CHO-K1 cells.
The aim of this study was to investigate the cytotoxic and genotoxic effect of copper extracts obtained from metallic copper in Chinese hamster ovary (CHO-K1) cell line using neutral red (NR), sister chromatid exchange (SCE), chromosomal aberrations (CA) and cell-cycle kinetics tests. Cells were cultured in Ham-F10 with different copper-containing extracts obtained after the immersion of copper disks for 1, 2, 3, 9, 12, 24, 48 and 72 h in culture medium. Results from cytotoxicity assay showed an inverted U-shape response evidenced in changes in lysosomal activity and mitotic index. The analysis of CA revealed an increase of abnormal metaphases for copper concentration (cCu) in the 5.67-7.42 mg/L dose-range (p<0.001). In addition, SCE frequencies were higher for treated cells when compared with controls in the 1.56-7.42 mg/L concentration range (p<0.001). The absence of metaphases indicated cytotoxicity for cCu≥10.85 mg/L. Results show that cells close to copper-containing materials releasing copper ions are susceptible to cytotoxic and genotoxic effects.
Grillo
C A
CA
INIFTA (Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas), UNLP, CONICET, Argentina.
Reigosa
M A
MA
Lorenzo de Mele
M Fernández
MF
D000818:Animals; D016466:CHO Cells / Q000187:drug effects*; D002869:Chromosome Aberrations / Q000187:drug effects; D003300:Copper / Q000633:toxicity*; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000494:pharmacology; D007477:Ions / Q000633:toxicity*; D007700:Kinetics; D008938:Mitosis; D009499:Neutral Red / Q000494:pharmacology; D012044:Regression Analysis; D012854:Sister Chromatid Exchange; D013054:Spectrophotometry, Atomic / Q000379:methods; D013997:Time Factors
D003470:Culture Media; D007477:Ions; D009499:Neutral Red; D003300:Copper