title: Bacterial leakage in root canals filled with calcium hydroxide paste associated with different vehicles.
abstract: This in vitro study evaluated, using a bacterial leakage model, whether intracanal medication with calcium hydroxide [Ca(OH)2]-based pastes prepared with different vehicles, has inhibitory effect on corono-apical leakage of bacteria. Forty instrumented human canines were dressed with Ca(OH)2 p.a. associated with: G1 = distilled water; G2 = polyethylene glycol (PG); G3 = PG + CMCP; and G4 = glycerin. Five teeth with intact crowns served as negative controls and 5 instrumented teeth without temporary dressing served as positive controls. All teeth were mounted in a 2-chamber apparatus and then exposed to human saliva for 63 days. Leakage was recorded when turbidity was observed in the lower chamber. Fifty percent of the samples of G1 and G2, 10% of G3 and 80% of G4 were fully contaminated after 9 weeks. Statistically significant differences were observed with paired comparisons G3 and G4 (p=0.0069), with G3 achieving better seal against bacterial leakage than G4.
authors.affiliation: ProClin Department, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Murad
authors.forename: Luiz Fernando
authors.initials: LF
authors.lastname: Fariniuk
authors.forename: Sandra
authors.initials: S
authors.lastname: Fidel
authors.forename: Rivail Antonio Sergio
authors.initials: RA
authors.lastname: Fidel
authors.forename: Luciana Moura
authors.initials: LM
authors.lastname: Sassone
mesh_terms: D000891:Anti-Infective Agents, Local / Q000008:administration & dosage; D002126:Calcium Hydroxide / Q000008:administration & dosage / Q000627:therapeutic use*; D002164:Camphor / Q000008:administration & dosage; D002733:Chlorophenols / Q000008:administration & dosage; D003763:Dental Leakage / Q000382:microbiology*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004337:Drug Carriers; D004338:Drug Combinations; D004492:Edetic Acid / Q000627:therapeutic use; D005990:Glycerol / Q000008:administration & dosage; D006801:Humans; D008422:Materials Testing; D014677:Pharmaceutical Vehicles; D011092:Polyethylene Glycols / Q000008:administration & dosage; D012388:Root Canal Irrigants / Q000008:administration & dosage / Q000627:therapeutic use*; D018915:Root Canal Preparation; D012463:Saliva / Q000382:microbiology; D016369:Smear Layer; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D012997:Solvents / Q000008:administration & dosage; D013997:Time Factors; D019227:Tooth Apex / Q000382:microbiology; D014867:Water
chemical_list: D000891:Anti-Infective Agents, Local; D002733:Chlorophenols; D004337:Drug Carriers; D004338:Drug Combinations; D014677:Pharmaceutical Vehicles; D012388:Root Canal Irrigants; D012997:Solvents; D014867:Water; D011092:Polyethylene Glycols; D002164:Camphor; C029804:camphorated parachlorophenol; D004492:Edetic Acid; D012973:Sodium Hypochlorite; D005990:Glycerol; D002126:Calcium Hydroxide