Assessment of islet graft survival using a 3.0-Tesla magnetic resonance scanner.
Some studies have recently described a magnetic resonance (MR) method for detection of iron-labeled islets transplanted into the liver. The aim of this work was to assess the survival of islet graft using a clinical 3.0-T scanner. Islets from Lewis rats were cultured in the presence of iron oxide nanoparticles. One thousand iron-labeled islets were transplanted into the portal vein of diabetic rats. Blood glucose levels were measured daily through day 14 post-transplantation. MR imaging of the same section of the liver was performed on 1, 3, 7, 10, and 14 days post-transplantation. The labeled islets were visualized by MR as distinct hypointensive spots distributed in the liver. There was a linear correlation between the relative value of delta R2* relaxometry multiplied by the cubic diameter (relative value of the iron volume, Ir) and blood glucose level on 14 days post-transplantation in allograft and isograft (P<0.05). The relative value of delta R2* relaxometry, diameter, and number of hypointensive spots could be calculated to assess the survival of the iron-labeled islet grafts. Assessment of iron-labeled islet grafts using a clinical 3.0-T magnetic resonance scanner represents a useful method that has potential for clinical use.
Jiao
Yang
Y
Shanghai Organ Transplantation Clinical Medical Center, the First People's Hospital affiliated Shanghai Jiao Tong University, Shanghai, China.
Peng
Zhi-Hai
ZH
Xing
Tong-Hai
TH
Qin
Jie
J
Zhong
Cui-Ping
CP
D000818:Animals; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods; D001786:Blood Glucose / Q000502:physiology; D003920:Diabetes Mellitus / Q000601:surgery*; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D004195:Disease Models, Animal; D006085:Graft Survival / Q000502:physiology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007501:Iron / Q000378:metabolism / Q000493:pharmacokinetics; D007515:Islets of Langerhans / Q000166:cytology* / Q000502:physiology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D011169:Portal Vein / Q000601:surgery; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
D001786:Blood Glucose; D007328:Insulin; D007501:Iron