title: Colony multiplex-tandem PCR for rapid, accurate identification of fungal cultures.
abstract: We developed a multiplex tandem PCR (MT-PCR) assay for the rapid identification of 16 fungi directly from culture. MT-PCR results were concordant with phenotypic identification for all cultures studied (n = 183). The colony MT-PCR assay was rapid (<2 h), sensitive, and specific in identifying fungal pathogens directly from primary isolation plates.
authors.affiliation: Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, New South Wales, Australia.
authors.forename: Anna
authors.initials: A
authors.lastname: Lau
authors.forename: Tania C
authors.initials: TC
authors.lastname: Sorrell
authors.forename: OkCha
authors.initials: O
authors.lastname: Lee
authors.forename: Keith
authors.initials: K
authors.lastname: Stanley
authors.forename: Catriona
authors.initials: C
authors.lastname: Halliday
mesh_terms: D005658:Fungi / Q000145:classification* / Q000302:isolation & purification*; D006801:Humans; D009181:Mycoses / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity