title: Inducible Expression of a Phytolacca heterotepala Ribosome-Inactivating Protein Leads to Enhanced Resistance Against Major Fungal Pathogens in Tobacco.
abstract: ABSTRACT Plant genetic engineering has long been considered a valuable tool to fight fungal pathogens because it would limit the economically costly and environmentally undesirable chemical methods of disease control. Ribosome-inactivating proteins (RIPs) are potentially useful for plant defense considering their antiviral and antimicrobial activities but their use is limited by their cytotoxic activity. A new gene coding for an RIP isolated from leaves of Phytolacca heterotepala was expressed in tobacco under the control of the wound-inducible promoter of the bean polygalacturonase-inhibiting protein I gene to increase resistance against different fungal pathogens, because an individual RIP isolated from P. heterotepala showed direct antifungal toxicity. Phenotypically normal transgenic lines infected with Alternaria alternata and Botrytis cinerea showed a significant reduction of leaf damage while reverse transcription-polymerase chain reaction and western analysis indicated the expression of the RIP transgene upon wounding and pathogen attack. This work demonstrates that use of a wound-inducible promoter is useful to limit the accumulation of antimicrobial phytotoxic proteins only in infected areas and that the controlled expression of the PhRIP I gene can be very effective to control fungal pathogens with different phytopathogenic actions.
authors.forename: Giandomenico
authors.initials: G
authors.lastname: Corrado
authors.forename: Pasquale Delli
authors.initials: PD
authors.lastname: Bovi
authors.forename: Rosalia
authors.initials: R
authors.lastname: Ciliento
authors.forename: Luciano
authors.initials: L
authors.lastname: Gaudio
authors.forename: Antimo
authors.initials: A
authors.lastname: Di Maro
authors.forename: Serena
authors.initials: S
authors.lastname: Aceto
authors.forename: Matteo
authors.initials: M
authors.lastname: Lorito
authors.forename: Rosa
authors.initials: R
authors.lastname: Rao