title: Quantitation of green fluorescent protein using a gel-based imaging method.
abstract: Green fluorescent protein (GFP) is a versatile reporter protein and has been widely used in biological research. However, its quantitation requires expensive equipment such as a spectrofluorometer. In the current study, a gel documentation imaging system using a native polyacrylamide gel for the quantitation of GFP was developed. The assay was evaluated for its precision, linearity, reproducibility, and sensitivity in the presence of Escherichia coli cells and was compared with the spectrofluorometric method. Using this newly established, gel-based imaging technique; the amount of GFP can be quantified accurately.
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Few Ne
authors.initials: FN
authors.lastname: Chew
authors.forename: Wen Siang
authors.initials: WS
authors.lastname: Tan
authors.forename: Tau Chuan
authors.initials: TC
authors.lastname: Ling
authors.forename: Chon Seng
authors.initials: CS
authors.lastname: Tan
authors.forename: Beng Ti
authors.initials: BT
authors.lastname: Tey
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D049452:Green Fluorescent Proteins