Embryonic stem cell/fibroblast hybrid cells with near-tetraploid karyotype provide high yield of chimeras.
Ten primary clones of hybrid cells were produced by the fusion of diploid embryonic stem (ES) cells, viz., line E14Tg2aSc4TP6.3 marked by green fluorescent protein (GFP), with diploid embryonic or adult fibroblasts derived from DD/c mice. All the hybrid clones had many characteristics similar to those of ES cells and were positive for GFP. Five hybrid clones having ploidy close to tetraploidy (over 80% of cells had 76-80 chromosomes) were chosen for the generation of chimeras via injection into C57BL blastocysts. These hybrid clones also contained microsatellites marking all ES cell and fibroblast chromosomes judging from microsatellite analysis. Twenty chimeric embryos at 11-13 days post-conception were obtained after injection of hybrid cells derived from two of three clones. Many embryos showed a high content of GFP-positive descendents of the tested hybrid cells. Twenty one adult chimeras were generated by the injection of hybrid cells derived from three clones. The contribution of GFP-labeled hybrid cells was significant and comparable with that of diploid E14Tg2aSc4TP6.3 cells. Cytogenetic and microsatellite analyses of cell cultures derived from chimeric embryos or adults indicated that the initial karyotype of the tested hybrid cells remained stable during the development of the chimeras, i.e., the hybrid cells were mainly responsible for the generation of the chimeras. Thus, ES cell/fibroblast hybrid cells with near-tetraploid karyotype are able to generate chimeras at a high rate, and many adult chimeras contain a high percentage of descendants of the hybrid cells.
Kruglova
A A
AA
Department of Developmental Genetics, Institute of Cytology and Genetics, Academy of Sciences of Russia (Siberian Branch), Lavrentiev Prospect 10, 630090, Novosibirsk, Russia.
Kizilova
E A
EA
Zhelezova
A I
AI
Gridina
M M
MM
Golubitsa
A N
AN
Serov
O L
OL
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D002678:Chimera; D033481:Chromosomes, Mammalian / Q000378:metabolism; D002999:Clone Cells / Q000201:enzymology; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D018398:Homeodomain Proteins / Q000378:metabolism; D006822:Hybrid Cells / Q000166:cytology*; D007621:Karyotyping; D034904:Lamin Type A / Q000378:metabolism; D008297:Male; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D011123:Polyploidy
D018398:Homeodomain Proteins; D034904:Lamin Type A; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D000469:Alkaline Phosphatase