[Effect of Helicobacter pylori-encoded CagA on biological behavior of gastric adenocarcinoma cells in vitro].
OBJECTIVE
To investigate the effect of Helicobacter pylori-encoded CagA on biological behavior of gastric adenocarcinoma AGS cells.


METHODS
With experiment-control system of the wild-type CagA positive strain and isogenic CagA negative mutant strain of Helicobacter pyroli (Hp) were used as control and experimental groups, respectively. The cell contact, migration and invasion were examined by light and electron microscopy and invasion assay.


RESULTS
The AGS cells infected by Hp strain with positive wild-type CagA showed more severely changed tight junction, wider intercellular space, loss of cell contacts, and higher migrating and invasive ability.


CONCLUSIONS
Hp CagA may lead to loss of cell contacting and higher migrating and invading ability of gastic cells, and accelerates the malignant progress of tumor.
Song
Xin
X
Department of Gastroenterology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Chen
Hui-Xin
HX
Chen
Jie
J
Bai
Ai-Ping
AP
Li
Xiao-Yan
XY
Chen
Wei
W
Chen
Min-Hu
MH
D000230:Adenocarcinoma / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005110:Extracellular Space; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D007365:Intercellular Junctions / Q000648:ultrastructure*; D009154:Mutation; D013274:Stomach Neoplasms / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori