Microfluidic aqueous two phase system for leukocyte concentration from whole blood.
Leukocytes from a whole blood sample were concentrated using a microfluidic aqueous two phase system (microATPS). Whole blood was simultaneously exposed to polyethylene glycol (PEG) and dextran (Dex) phase streams and cells were partitioned based on their differential affinity for the streams. The laminar flow characteristic of microfluidic devices was used to create zero, one, and two stable interfaces between the polymer streams. Three different patterns of three polymer streams each were evaluated for their effectiveness in concentrating leukocytes: immiscible PEG-PEG-Dex, immiscible Dex-PEG-Dex, and miscible PEG-PBS-Dex. The most effective configuration was the Dex-PEG-Dex stream pattern which on average increased the ratio of leukocytes to erythrocytes by a factor of 9.13 over unconcentrated blood.
Soohoo
Jeffrey R
JR
The Joint Department of Biomedical Engineering, North Carolina State University and the University of North Carolina at Chapel Hill, Raleigh, NC 27695-7115, USA. jrsoohoo@ncsu.edu
Walker
Glenn M
GM
D001781:Blood Component Removal / Q000295:instrumentation* / Q000379:methods*; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007962:Leukocytes / Q000166:cytology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D044367:Phase Transition