Design of a novel molecular beacon: modification of the stem with artificially genetic alphabet.
A molecular beacon that incorporates components of an artificially expanded genetic information system (Aegis) in its stem is shown not to be opened by unwanted stem invasion by adventitious standard DNA; this should improve the "darkness" of the beacon in real-world applications.
Sheng
Pinpin
P
Department of Chemistry and UF Genetics Institute, Shands Cancer Center, Center for Research at Bio/nano Interface and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.
Yang
Zunyi
Z
Kim
Youngmi
Y
Wu
Yanrong
Y
Tan
Weihong
W
Benner
Steven A
SA
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry; D013816:Thermodynamics
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D012313:RNA; D004247:DNA