Masculinization of XX Drosophila transgenic flies expressing the Ceratitis capitata DoublesexM isoform.
The Doublesex (DSX) transcription factor regulates somatic sexual differentiation in Drosophila melanogaster. Female and male isoforms (DSXF and DSXM) are produced due to sex-specific RNA splicing. Here we show that in the distantly related dipteran Ceratitis capitata, the DSXM male-specific isoform is conserved and able to induce masculinization of both somatic and germline tissues when ectopically expressed in XX Drosophila transgenic individuals.
Saccone
Giuseppe
G
Department of Biological Sciences, Genetics and Molecular Biology Section, University of Naples Federico II, Naples, Italy.
Salvemini
Marco
M
Pane
Attilio
A
Polito
Lino C
LC
D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D033622:Ceratitis capitata / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development*; D005260:Female; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D020033:Protein Isoforms / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation / Q000235:genetics; D013045:Species Specificity
D020413:3' Untranslated Regions; D017931:DNA Primers; D004268:DNA-Binding Proteins; C070369:DSX protein, Drosophila; D029721:Drosophila Proteins; D019476:Insect Proteins; D020033:Protein Isoforms