title: Peripheral blood flow cytometry based diagnosis in primary cutaneous aggressive epidermotropic CD8+ T-cell lymphoma: implications for more extensive use.
abstract: Primary cutaneous epidermotropic CD8-positive T-cell lymphoma represents an aggressive form of T-cell cutaneous lymphomas. Diagnosis is based on biopsies obtained from skin lesions. Here, we would like to report a case diagnosed by using flow cytometry performed on peripheral blood mononuclear cells. Moreover, an important finding was the difference in the results on targeting the CD8 antigen by using two different commercially available monoclonal antibodies. Perhaps, more than one antibody should be used in primary cutaneous aggressive epidermotropic CD8-positive T-cell lymphomas, in order to be more accurate in the diagnosis and so in the classification of such diseases.
authors.affiliation: Department of Haematology, University Hospital of Ioannina, Greece.
authors.forename: Leonidas
authors.initials: L
authors.lastname: Benetatos
authors.forename: Lefkothea
authors.initials: L
authors.lastname: Dova
authors.forename: Gerasimos
authors.initials: G
authors.lastname: Baxevanos
authors.forename: Nikolaos I
authors.initials: NI
authors.lastname: Kolaitis
authors.forename: Konstantinos L
authors.initials: KL
authors.lastname: Bourantas
authors.forename: Georgios
authors.initials: G
authors.lastname: Vartholomatos
mesh_terms: D000369:Aged, 80 and over; D000906:Antibodies; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D016827:CD8 Antigens / Q000032:analysis*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D003937:Diagnosis, Differential; D018450:Disease Progression; D004817:Epidermis / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D017809:Fatal Outcome; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D016410:Lymphoma, T-Cell, Cutaneous / Q000175:diagnosis* / Q000276:immunology / Q000503:physiopathology; D008297:Male; D009361:Neoplasm Invasiveness / Q000175:diagnosis / Q000276:immunology / Q000503:physiopathology; D011237:Predictive Value of Tests; D011379:Prognosis; D012867:Skin / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D012871:Skin Diseases / Q000175:diagnosis* / Q000276:immunology / Q000503:physiopathology
chemical_list: D000906:Antibodies; D015415:Biomarkers; D016827:CD8 Antigens