Validation of EST-derived STS markers localized on Qfhs.ndsu-3BS for Fusarium head blight resistance in wheat using a 'Wangshuibai' derived population.
A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight (FHB) in wheat, have been previously identified in the 'Sumai 3'/'Stoa' population. In this study, we used a 'Wangshuibai' (resistant) /'Seri82' (susceptible) derived population, linkage group, QTL, and quantitative gene expression analysis to assess the genetic background dependence and stability of the EST-derived STS markers for use in marker aided selection to improve FHB resistance in wheat. Based on our results, a QTL in the map interval of Xsts3B-138_1-Xgwm493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16% of the phenotypic variation. BLASTN analysis indicated that Xsts3B-138_1 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 in 'Wangshuibai' at 96 h after inoculation was 2.6 times higher than 'Seri82'. Our results underlined that EST-derived STS3B-138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat.
Naji
Amir Mohammad
AM
Department of Agronomy and Crop Breeding, Shahed University of Tehran, Tehran 3319118651, Iran.
Moghaddam
Mohammad
M
Ghaffari
Mohammad Reza
MR
Irandoost
Hashem Pour
HP
Farsad
Laleh Karimi
LK
Pirseyedi
Seyed Mostafa
SM
Mohammadi
Seyed Abolghasem
SA
Ghareyazie
Behzad
B
Mardi
Mohsen
M
D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D005670:Fusarium / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D010935:Plant Diseases / Q000382:microbiology*; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci / Q000235:genetics*; D016324:Sequence Tagged Sites; D014908:Triticum / Q000145:classification / Q000235:genetics* / Q000382:microbiology*
D005819:Genetic Markers