title: Growing steers grazing high versus low endophyte (Neotyphodium coenophialum)-infected tall fescue have reduced serum enzymes, increased hepatic glucogenic enzymes, and reduced liver and carcass mass.
abstract: It is well established that grazing Neotyphodium coenophialum-infected forages results in reduced BW gain and serum prolactin concentrations of cattle. The objective of this study was to determine the potential effects of toxic endophyte-infected tall fescue consumption on blood metabolites, carcass characteristics, and content of proteins critical for AA metabolism in the liver, kidney, and LM tissue of growing steers. Steers grazed a low toxic endophyte (LE; 0.023 microg/g ergot alkaloids) tall fescue-mixed grass pasture (n = 9; BW = 266 +/- 10.9 kg; 5.7 ha) or a high toxic endophyte (HE; 0.746 microg/g of ergot alkaloids) tall fescue pasture (n = 10; BW = 267 +/- 14.5 kg; 5.7 ha) from June 14 through at least September 11 (> or =89 d). No difference was observed for BW (P < 0.10) for the overall 85-d growth period. Also, no differences were observed for ribeye area/100 kg of HCW (P > 0.91), backfat (P > 0.95), or backfat/100 kg of HCW (P > 0.67). However, ADG (P < 0.01), final BW (P < 0.05), HCW (P < 0.01), dressing percentage (P < 0.01), ribeye area (P < 0.01), whole liver wet weight (P < 0.01), and whole liver wet weight/100 kg of end BW (P < 0.01) were greater for LE steers than HE steers. After 85 d of grazing, serum concentrations of alkaline phosphatase (P < 0.05), alanine aminotransferase (P < 0.01), aspartate aminotransferase (P < 0.03), cholesterol (P < 0.01), lactate dehydrogenase (P < 0.01), and prolactin (P < 0.01) were less for HE than LE steers. At slaughter, hepatic content of cytosolic phosphoenolpyruvate carboxykinase (P < 0.01) was greater in HE steers than LE steers. Hepatic content of aspartate aminotransferase (P < 0.01) also was greater, whereas renal and LM content were not (P > or = 0.42). No differences (P > or = 0.15) were observed for hepatic, renal, and LM content of alanine aminotransferase, glutamate dehydrogenase, glutamine synthetase, and 3 glutamate transport proteins. These data indicate that the HE steers displayed classic endophyte toxicity symptoms for growth and blood variables, classic symptoms that were concomitant with novelly identified altered glucogenic capacity of the liver and decreases in carcass characteristics.
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington 40546-0215, USA.
authors.forename: K R
authors.initials: KR
authors.lastname: Brown
authors.forename: G A
authors.initials: GA
authors.lastname: Anderson
authors.forename: K
authors.initials: K
authors.lastname: Son
authors.forename: G
authors.initials: G
authors.lastname: Rentfrow
authors.forename: L P
authors.initials: LP
authors.lastname: Bush
authors.forename: J L
authors.initials: JL
authors.lastname: Klotz
authors.forename: J R
authors.initials: JR
authors.lastname: Strickland
authors.forename: J A
authors.initials: JA
authors.lastname: Boling
authors.forename: J C
authors.initials: JC
authors.lastname: Matthews
mesh_terms: D026905:Amino Acid Transport Systems / Q000032:analysis; D000821:Animal Feed / Q000032:analysis / Q000382:microbiology*; D000818:Animals; D001773:Blood Cells / Q000166:cytology; D001835:Body Weight / Q000502:physiology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000201:enzymology / Q000503:physiopathology*; D004798:Enzymes / Q000097:blood / Q000378:metabolism; D004881:Ergotism / Q000201:enzymology / Q000503:physiopathology / Q000662:veterinary*; D031751:Festuca / Q000382:microbiology*; D008099:Liver / Q000503:physiopathology*; D008297:Male; D008460:Meat / Q000592:standards; D008903:Minerals / Q000032:analysis / Q000097:blood; D055328:Neotyphodium / Q000502:physiology*; D009929:Organ Size / Q000502:physiology; D011897:Random Allocation
chemical_list: D026905:Amino Acid Transport Systems; D004798:Enzymes; D008903:Minerals