Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA.
Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.
Groot
Masja Nierop
MN
Food Technology Centre, Wageningen UR, P.O. Box 17, 6700 AA Wageningen, The Netherlands. masja.nieropgroot@wur.nl
Nieboer
Frank
F
Abee
Tjakko
T
D001407:Bacillus / Q000235:genetics*; D015280:DNA Restriction-Modification Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism*; D018274:Electroporation; D008745:Methylation; D010957:Plasmids / Q000378:metabolism*; D014169:Transformation, Bacterial
D015280:DNA Restriction-Modification Enzymes; D004269:DNA, Bacterial