title: Zebrafish runx1 promoter-EGFP transgenics mark discrete sites of definitive blood progenitors.
abstract: The transcription factor Runx1 is essential for the development of definitive hematopoietic stem cells (HSCs) during vertebrate embryogenesis and is transcribed from 2 promoters, P1 and P2, generating 2 major Runx1 isoforms. We have created 2 stable runx1 promoter zebrafish-transgenic lines that provide insight into the roles of the P1 and P2 isoforms during the establishment of definitive hematopoiesis. The Tg(runx1P1:EGFP) line displays fluorescence in the posterior blood island, where definitive erythromyeloid progenitors develop. The Tg(runx1P2:EGFP) line marks definitive HSCs in the aorta-gonad-mesonephros, with enhanced green fluorescent protein-labeled cells later populating the pronephros and thymus. This suggests that a function of runx1 promoter switching is associated with the establishment of discrete definitive blood progenitor compartments. These runx1 promoter-transgenic lines are novel tools for the study of Runx1 regulation and function in normal and malignant hematopoiesis. The ability to visualize and isolate fluorescently labeled HSCs should contribute to further elucidating the complex regulation of HSC development.
authors.affiliation: Department of Molecular Medicine & Pathology, School of Medical Sciences, University of Auckland, Auckland, New Zealand.
authors.forename: Enid Yi Ni
authors.initials: EY
authors.lastname: Lam
authors.forename: Jackie Y M
authors.initials: JY
authors.lastname: Chau
authors.forename: Maggie L
authors.initials: ML
authors.lastname: Kalev-Zylinska
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Fountaine
authors.forename: R Scott
authors.initials: RS
authors.lastname: Mead
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hall
authors.forename: Philip S
authors.initials: PS
authors.lastname: Crosier
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Crosier
authors.forename: Maria Vega
authors.initials: MV
authors.lastname: Flores
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D019070:Cell Lineage; D050676:Core Binding Factor Alpha 2 Subunit / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D015672:Erythroid Precursor Cells / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006410:Hematopoiesis; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008650:Mesonephros / Q000166:cytology / Q000196:embryology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020033:Protein Isoforms; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000502:physiology*
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D020033:Protein Isoforms; D029961:Zebrafish Proteins; C485184:enhanced green fluorescent protein; C472526:runx1 protein, zebrafish; D049452:Green Fluorescent Proteins