Evaluation of the immunogenicity of replication-competent V-knocked-out and replication-defective F-deleted Sendai virus vector-based vaccines in macaques.
Viral vectors are promising vaccine tools for eliciting antigen-specific T-cell responses. We previously showed the potential of recombinant Sendai virus (SeV) vectors to induce virus-specific T-cell responses in macaque AIDS models. Here, we have evaluated the immunogenicity of replication-competent V-knocked-out and replication-defective F-deleted SeV vectors in macaques. Intranasal replication-competent and replication-defective SeV immunizations both elicited robust systemic antigen-specific T-cell responses, whereas the responses induced by the former were more durable than those by the latter. However, even the latter-induced T-cell responses remained detectable in a local, retropharyngeal lymph node two months after the immunization. These findings are useful for establishment of a vaccine protocol using SeV vectors.
Takeda
Akiko
A
The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
Igarashi
Hiroko
H
Kawada
Miki
M
Tsukamoto
Tetsuo
T
Yamamoto
Hiroyuki
H
Inoue
Makoto
M
Iida
Akihiro
A
Shu
Tsugumine
T
Hasegawa
Mamoru
M
Matano
Tetsuro
T
D000281:Administration, Intranasal; D000818:Animals; D015683:Gene Products, gag / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics* / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008252:Macaca fascicularis; D008253:Macaca mulatta; D009297:Nasal Mucosa / Q000166:cytology / Q000276:immunology; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000276:immunology; D029082:Sendai virus / Q000235:genetics* / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics* / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology*; D014779:Virus Replication / Q000235:genetics* / Q000276:immunology*
D015683:Gene Products, gag; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic