[Correlation of Epstein-Barr virus infection and childhood lymphoma].
OBJECTIVE
To investigate the correlation of Epstein-Barr virus (EBV) infection and childhood lymphoma.


METHODS
Paraffin-embedded specimens of lymphoma collected between 1996 and 2005, including 36 Hodgkin lymphomas (HL) and 51 non-Hodgkin lymphomas (NHL), were included in this study. Paraffin-embedded specimens of reactive hyperplasia of lymph nodes (RL) collected during the same period were used as controls. Immunohistochemical (IHC) assay was used to detect EBV-LMP1 and in situ hybridization (ISH) to detect EBV-EBERs.


RESULTS
EBV was detected in 72.2% (26/36) of the Hodgkin lymphomas, 15.7% (8/51) of the non-Hodgkin lymphomas and 33.3% (15/45) of the reactive hyperplasia of lymph nodes. There was a significant difference among Hodgkin lymphoma, non-Hodgkin lymphoma and RL (P = 0. 000).


CONCLUSIONS
Childhood Hodgkin lymphoma is closely correlated with Epstein-Barr virus infection. However, the low rate of EBV infection detected in childhood non-Hodgkin lymphoma might be due to heterogeneous distribution of pathological types in this study.
Xie
Zheng-De
ZD
Laboratory of Virology, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
Wang
Lin
L
Lu
Di
D
Zhou
Chun-Ju
CJ
Shen
Kun-Ling
KL
D002648:Child; D002675:Child, Preschool; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000378:metabolism; D005260:Female; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000150:complications / Q000378:metabolism / Q000821:virology*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000150:complications / Q000378:metabolism / Q000821:virology*; D008297:Male; D019310:Pseudolymphoma / Q000150:complications / Q000378:metabolism / Q000821:virology; D012367:RNA, Viral / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000378:metabolism*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; C099537:Epstein-Barr virus encoded RNA 1; C106732:Epstein-Barr virus encoded RNA 2; D012367:RNA, Viral; D014763:Viral Matrix Proteins