title: Polymorphism of the pig 17beta-hydroxysteroid dehydrogenase type1 (HSD17B1) gene and its association with reproductive traits.
abstract: 17beta-Hydroxysteroid dehydrogenase type 1 (HSD17B1) is a key enzyme of 17beta-estradiol biosynthesis, which might play an important role in follicular development of the ovary. In this study, we isolated the complete coding sequence of porcine HSD17B1 gene and its unique intron sequences of porcine HSD17B1 gene, identified a single nucleotide polymorphism (SNP: A/C) in intron 4, and developed a PCR-MvaI-RFLP genotyping assay. Association of the SNP and litter size was assessed in two populations (purebred Large White and a experimental synthetic Line (DIV) sows). Statistical analysis demonstrated that, in the first parity, AC animals in experimental synthetic Line (DIV) sows had 0.52 more piglets born compared to the CC animals (P<0.05). In the all parities, pigs with the AA genotype had an additional 1.11 and 0.96 piglets born alive compared to the CC animals (P<0.05) in both experimental synthetic Line (DIV) and purebred Large White, respectively. Experimental synthetic Line (DIV) sows inheriting the AC genotype had additional 0.84 piglets born alive compared to the CC animals (P<0.01) in all parities. In addition, significant additive effect of -0.55+/-0.24 piglets/litter and -0.48+/-0.22 piglets/litter on piglet born alive was detected in both experimental synthetic Line (DIV) sows and purebred Large White lines (P<0.05), respectively. Therefore, HSD17B1 gene was significantly associated with litter size in two populations and could be a useful molecular marker in selection for increasing litter size in pigs.
authors.affiliation: Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, PR China. liulinqingllq@yahoo.com.cn
authors.forename: L Q
authors.initials: LQ
authors.lastname: Liu
authors.forename: F E
authors.initials: FE
authors.lastname: Li
authors.forename: C Y
authors.initials: CY
authors.lastname: Deng
authors.forename: B
authors.initials: B
authors.lastname: Zuo
authors.forename: R
authors.initials: R
authors.lastname: Zheng
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Xiong
mesh_terms: D000818:Animals; D001947:Breeding; D004960:Estradiol Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D005819:Genetic Markers; D019382:Human Growth Hormone; D011110:Polymorphism, Genetic; D012098:Reproduction / Q000235:genetics*; D013552:Swine / Q000235:genetics* / Q000502:physiology*
chemical_list: D005819:Genetic Markers; D019382:Human Growth Hormone; D004960:Estradiol Dehydrogenases