title: iTRAQ analysis of Singapore grouper iridovirus infection in a grouper embryonic cell line.
abstract: We report, here, the first proteomics study of a grouper embryonic cell line (GEC) infected by Singapore grouper iridovirus (SGIV). The differential proteomes of GEC with and without viral infection were studied and quantified with iTRAQ labelling followed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Forty-nine viral proteins were recognized, of which 11 were identified for the first time. Moreover, 743 host proteins were revealed and classified into 218 unique protein groups. Fourteen host proteins were upregulated and five host proteins were downregulated upon viral infection. The iTRAQ analysis of SGIV infection in GEC provides an insight to viral and host gene products at the protein level. This should facilitate further study and the understanding of virus-host interactions, molecular mechanisms of viral infection and pathogenesis.
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Li Ming
authors.initials: LM
authors.lastname: Chen
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Bich Ngoc
authors.initials: BN
authors.lastname: Tran
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Teck Kwang
authors.initials: TK
authors.lastname: Lim
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Choy-Leong
authors.initials: CL
authors.lastname: Hew
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D005393:Fish Diseases / Q000821:virology; D017781:Genes, Immediate-Early; D005814:Genes, Viral; D017994:Iridovirus / Q000235:genetics* / Q000472:pathogenicity; D007553:Isotope Labeling / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D010473:Perciformes / Q000196:embryology / Q000821:virology; D025821:Polyubiquitin / Q000737:chemistry / Q000235:genetics; D012846:Singapore; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014777:Virus Diseases / Q000821:virology
chemical_list: D014764:Viral Proteins; D025821:Polyubiquitin