title: Calcium loading capacity and morphological changes in mitochondria in an ischemic preconditioned model.
abstract: The concept of the mitochondrial permeability transition (mPT) has been used to explain cell death induced by calcium deregulation, which is in turn induced by a disruption in the mitochondrial loading capacity of cytosolic calcium (CLC). Whether mitochondria have specific morphologies representing the CLC and the mPT remains controversial. We examined ultrastructural changes in the mitochondria of cultured hippocampal neurons preconditioned with oxygen-glucose deprivation (OGD) for 30 min (30OGD) or 120 min (120OGD). The CLC was then evaluated using simultaneous imaging of the mitochondrial and plasma Ca++ concentrations after the induction of Ca++ influx by the application of glutamate. In the 30OGD group, the CLC increased as the mitochondria rapidly reacted to the increase in plasma Ca++, which was soon cleared. In the 120OGD group, however, the CLC was disturbed because the mitochondrial uptake of Ca was blunted, and the plasma Ca++ was not cleared after glutamate application. We classified the specific morphological changes in the mitochondria according to a previously reported classification. Rounded mitochondria with scarce interior content were observed in the 120OGD group, a model of prolonged lethal OGD, and disruptions in the mitochondrial outer membrane were frequently confirmed, suggesting mPT. The 30OGD group, a model of enhanced CLC in preconditioned neurons, was characterized by round mitochondria with condensed matrices. After glutamate application, the mitochondria became even more rounded with expanded matrices, and outer membrane disruptions were occasionally seen. Our observations suggest that subpopulations of mitochondria with specific morphologies are linked to the CLC and mPT.
authors.affiliation: Department of Anesthesiology, Kyorin University, School of Medicine, Japan. iijmt@kyorin-u.ac.jp
authors.forename: Takehiko
authors.initials: T
authors.lastname: Iijima
authors.forename: Kensuke
authors.initials: K
authors.lastname: Tanaka
authors.forename: Sachie
authors.initials: S
authors.lastname: Matsubara
authors.forename: Hayato
authors.initials: H
authors.lastname: Kawakami
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Mishima
authors.forename: Kei
authors.initials: K
authors.lastname: Suga
authors.forename: Kimio
authors.initials: K
authors.lastname: Akagawa
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Iwao
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005456:Fluorescent Dyes; D005971:Glutamates / Q000494:pharmacology; D002534:Hypoxia, Brain / Q000473:pathology / Q000523:psychology; D019194:Ischemic Preconditioning; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure*; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008959:Models, Neurological; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005456:Fluorescent Dyes; D005971:Glutamates; D002118:Calcium