title: Rice dwarf virus is engulfed into and released via vesicular compartments in cultured insect vector cells.
abstract: Vector insect cells infected with Rice dwarf virus had vesicular compartments containing viral particles located adjacent to the viroplasm when examined by transmission electron and confocal microscopy. Such compartments were often at the periphery of infected cells. Inhibitors of vesicular transport, brefeldin A and monensin, and an inhibitor of myosin motor activity, butanedione monoxime, abolished the formation of such vesicles and prevented the release of viral particles from infected cells without significant effects on virus multiplication. Furthermore, the actin-depolymerizing drug, cytochalasin D, inhibited the formation of actin filaments without significantly interfering with formation of vesicular compartments and the release of viruses from treated cells. These results together revealed intracellular vesicular compartments as a mode for viral transport in and release from insect vector cells infected with a plant-infecting reovirus.
authors.affiliation: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors.forename: Taiyun
authors.initials: T
authors.lastname: Wei
authors.affiliation: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hibino
authors.affiliation: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Omura
mesh_terms: D000818:Animals; D020126:Brefeldin A / Q000494:pharmacology; D007303:Insect Vectors; D007313:Insecta / Q000821:virology; D018613:Microscopy, Confocal; D009218:Myosins / Q000187:drug effects / Q000502:physiology; D012275:Oryza / Q000821:virology*; D012087:Reoviridae / Q000302:isolation & purification / Q000502:physiology*; D014764:Viral Proteins / Q000187:drug effects / Q000502:physiology; D014780:Viruses
chemical_list: D014764:Viral Proteins; D020126:Brefeldin A; D009218:Myosins