title: A unique substitution at position 333 on the glycoprotein of rabies virus street strains isolated from non-hematophagous bats in Brazil.
abstract: The amino acid R or K at position 333 on the glycoprotein of the rabies virus is considered necessary for virulence in adult mice. Although some exceptions exist, substitution at this position causes expression of a phenotype that is either less pathogenic or non-virulent. To date, such substitutions have only been found in fixed strains of rabies virus. In this study, the authors found 333H, 333N, and 333Q substitutions at this position in rabies virus street strains isolated from non-hematophagous bats in Brazil. These strains showed pathogenicity and lethality on passage using adult mice with the intracerebral route and were confirmed rabies-positive by immunofluorescent assay. This suggests that these strains maintain virulence. Our findings indicate that rabies virus street strains with these substitutions exist in the field and may result in infection cycles.
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo, Japan. gosato@nih.go.jp
authors.forename: G
authors.initials: G
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: N
authors.initials: N
authors.lastname: Motizuki
authors.forename: S
authors.initials: S
authors.lastname: Hirano
authors.forename: T
authors.initials: T
authors.lastname: Itou
authors.forename: E M S
authors.initials: EM
authors.lastname: Cunha
authors.forename: F H
authors.initials: FH
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics*; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D001938:Brazil; D002685:Chiroptera / Q000821:virology*; D006023:Glycoproteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D012367:RNA, Viral / Q000235:genetics; D011818:Rabies / Q000821:virology; D011820:Rabies virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*; D014774:Virulence
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus