Various oils and detergents enhance the microbial production of farnesol and related prenyl alcohols.
The object of this research was improvement of prenyl alcohol production with squalene synthase-deficient mutant Saccharomyces cerevisiae ATCC 64031. On screening of many kinds of additives, we found that oils and detergents significantly enhanced the extracellular production of prenyl alcohols. Soybean oil showed the most prominent effect among the additives tested. Its effect was accelerated by a high concentration of glucose in the medium. The combination of these cultivation conditions led to the production of more than 28 mg/l of farnesol in the soluble fraction of the broth. The addition of these compounds to the medium was an effective method for large-scale production of prenyl alcohols with microorganisms.
Muramatsu
Masayoshi
M
Bio Research Laboratory, Toyota Motor Corporation, 1 Toyota-cho, Toyota, Aichi 471-8572, Japan.
Ohto
Chikara
C
Obata
Shusei
S
Sakuradani
Eiji
E
Shimizu
Sakayu
S
D003902:Detergents / Q000494:pharmacology*; D005204:Farnesol / Q000378:metabolism*; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009387:Neoprene / Q000378:metabolism*; D010938:Plant Oils / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
D003902:Detergents; D010938:Plant Oils; C016077:prenyl; D005204:Farnesol; D009387:Neoprene; D013186:Farnesyl-Diphosphate Farnesyltransferase