Effect of ligand activation of peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) in human lung cancer cell lines.
There is compelling evidence that peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) mediates terminal differentiation and is associated with inhibition of cell growth. However, it was recently suggested that growth of two human lung cancer cell lines is enhanced by PPARbeta/delta. The goal of the present study was to provide insight in resolving this controversy. Therefore, the effect of ligand activation of PPARbeta/delta in A549 and H1838 human lung cancer cell lines was examined using two high affinity PPARbeta/delta ligands. Ligand activation of PPARbeta/delta caused up-regulation of a known PPARbeta/delta target gene, angiopoietin-like 4 (Angptl4) but did not influence expression of phosphatase and tensin homolog (PTEN) or phosphorylation of protein kinase B (Akt), and did not affect cell growth. Results from this study demonstrate that two human lung cancer cell lines respond to ligand activation of PPARbeta/delta by modulation of target gene expression (Angptl4), but fail to exhibit significant modulation of cell proliferation.
He
Pengfei
P
Department of Veterinary and Biomedical Sciences and The Center of Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, USA.
Borland
Michael G
MG
Zhu
Bokai
B
Sharma
Arun K
AK
Amin
Shantu
S
El-Bayoumy
Karam
K
Gonzalez
Frank J
FJ
Peters
Jeffrey M
JM
D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008175:Lung Neoplasms / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D051058:Oncogene Protein v-akt / Q000378:metabolism; D047494:PPAR delta / Q000378:metabolism*; D048068:PPAR-beta / Q000378:metabolism*; D010766:Phosphorylation
D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D020662:Guanine Nucleotide Exchange Factors; D008024:Ligands; D009687:Nuclear Proteins; D047494:PPAR delta; D048068:PPAR-beta; C499303:ran-3 protein, C elegans; D051058:Oncogene Protein v-akt