title: Natural feed additive of Macleaya cordata: safety assessment in rats a 90-day feeding experiment.
abstract: Macleaya cordata (Willd.) (Papaveraceae) is used as an active component in the natural feed additive Sangrovit. Sangrovit contains mixture of the intact aerial parts and the fraction of quaternary benzo[c]phenanthridine alkaloids from M. cordata (FQBA). In a 90-day pilot toxicity trial, Sangrovit and the FQBA were tested for safety. Male Wistar rats were fed for 90 days with 100, 7000 or 14000mg of Sangrovit or 600mg of FQBA in 1kg of feed. Body and organ weights, clinical chemistry and hematology markers, oxidative stress parameters, morphological structure of tongue, liver, ileum, kidney and heart samples, and total cytochrome P450 in liver were monitored. The results showed no statistically significant alterations in any parameter between control and treated animals, except for the group treated with 14000ppm Sangrovit that resulted in elevation of reduced glutathione level and superoxide dismutase activity in liver.
authors.affiliation: Department of Medical Chemistry and Biochemistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
authors.forename: Adela
authors.initials: A
authors.lastname: Zdarilova
authors.forename: Eva
authors.initials: E
authors.lastname: Vrublova
authors.forename: Jitka
authors.initials: J
authors.lastname: Vostalova
authors.forename: Borivoj
authors.initials: B
authors.lastname: Klejdus
authors.forename: David
authors.initials: D
authors.lastname: Stejskal
authors.forename: Jitka
authors.initials: J
authors.lastname: Proskova
authors.forename: Pavel
authors.initials: P
authors.lastname: Kosina
authors.forename: Alena
authors.initials: A
authors.lastname: Svobodova
authors.forename: Rostislav
authors.initials: R
authors.lastname: Vecera
authors.forename: Jan
authors.initials: J
authors.lastname: Hrbac
authors.forename: Drahomira
authors.initials: D
authors.lastname: Cernochova
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Vicar
authors.forename: Jitka
authors.initials: J
authors.lastname: Ulrichova
authors.forename: Vilim
authors.initials: V
authors.lastname: Simanek
mesh_terms: D000821:Animal Feed / Q000032:analysis / Q000633:toxicity*; D000818:Animals; D053119:Benzophenanthridines / Q000032:analysis; D001772:Blood Cell Count; D001835:Body Weight / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000378:metabolism; D004032:Diet; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000633:toxicity*; D004435:Eating; D005243:Feces / Q000737:chemistry; D005503:Food Additives / Q000032:analysis / Q000633:toxicity*; D007546:Isoquinolines / Q000032:analysis; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D028101:Papaveraceae / Q000737:chemistry*; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D053119:Benzophenanthridines; D004365:Drugs, Chinese Herbal; D005503:Food Additives; D007546:Isoquinolines; C534469:sangrovit; D003577:Cytochrome P-450 Enzyme System; C005705:sanguinarine; C016299:chelerythrine