Cloning, expression and evaluation of the efficacy of a recombinant Baylisascaris schroederi Bs-Ag3 antigen in mice.
The gene Bs-Ag3 enconding a antigen of 37kDa from Baylisascaris schroederi (giant panda isolates), as well as the recombinant Bs-Ag3, obtained by cloning and expression of the Bs-Ag3 gene in heterologous host Escherichia coli BL-21 (DE3), were used to evaluate their ability to induce immune protective responses in BALB/c mice against L3-challenge infection in a mouse-B. schroederi model. There was a significant reduction (62.91%) of mice vaccinated with rBs-Ag3 coupled with Freund's complete adjuvant (FCA) in recovery of challenged B. schroederi L3 compared with either controls or mice vaccinated with FCA alone. Our data indicate recombinant Bs-Ag3 may be a potential target as a vaccine antigen for giant panda ascariasis.
Wang
Tao
T
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
He
Guangzhi
G
Yang
Guangyou
G
Fei
Yuxiang
Y
Zhang
Zhihe
Z
Wang
Chengdong
C
Yang
Zhi
Z
Lan
Jingchao
J
Luo
Li
L
Liu
Li
L
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D017191:Ascaridida Infections / Q000276:immunology* / Q000469:parasitology / Q000662:veterinary*; D001199:Ascaridoidea / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics; D007814:Larva; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics / Q000276:immunology; D018510:RNA, Helminth / Q000096:biosynthesis / Q000302:isolation & purification; D001503:Ursidae / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
D000947:Antigens, Helminth; D007074:Immunoglobulin G; D018510:RNA, Helminth; D014614:Vaccines, Synthetic