Genomic characterization of Ralstonia solanacearum phage phiRSB1, a T7-like wide-host-range phage.
PhiRSB1 is a wide-host-range, T7-like bacteriophage that infects and efficiently lyses the phytopathogenic bacterium Ralstonia solanacearum. The phiRSB1 genome comprises 43,079 bp of double-stranded DNA (61.7% G+C) with 325-bp terminal repeats and contains 47 open reading frames. Strong activity of tandem early promoters and wide specificity of phage promoters of phiRSB1 were demonstrated.
Kawasaki
Takeru
T
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
Shimizu
Mio
M
Satsuma
Hideki
H
Fujiwara
Akiko
A
Fujie
Makoto
M
Usami
Shoji
S
Yamada
Takashi
T
D017123:Bacteriophage T7 / Q000145:classification / Q000235:genetics; D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017902:Podoviridae / Q000145:classification / Q000235:genetics; D011401:Promoter Regions, Genetic; D043368:Ralstonia solanacearum / Q000821:virology*; D014158:Transcription, Genetic; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins