Simultaneous quantification of plant glyceroglycolipids including sulfoquinovosyldiacylglycerol by HPLC-ELSD with binary gradient elution.
Membrane lipids of photosynthetic organisms consist of glycerophospholipids and glyceroglycolipids. We investigated a method for the simultaneous quantitative analysis of neutral and acidic lipids using HPLC-ELSD, and quantified monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulfoquinovosyldiacylglycerol (SQDG). Ten complex lipid classes were separated with a binary gradient system consisting of chloroform and methanol-acetone-water-acetic acid (30:60:9:1, v/v/v/v) with 0.3% triethylamine (pH 4), and were eluted within 16 min. The contents of SQDG in ten edible plants ranged from 3 to 101 mg/100 g, and were positively correlated to the neutral glyceroglycolipids contents.
Yunoki
Keita
K
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan. yunoki@obihiro.ac.jp
Sato
Mayumi
M
Seki
Kazuto
K
Ohkubo
Takeshi
T
Tanaka
Yukihisa
Y
Ohnishi
Masao
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry*; D006017:Glycolipids / Q000032:analysis* / Q000737:chemistry*
D005227:Fatty Acids; D006017:Glycolipids; C013694:sulfoquinovosyl diglyceride; D005990:Glycerol