title: Linking SNPs to CAG repeat length in Huntington's disease patients.
abstract: Allele-specific silencing using small interfering RNAs targeting heterozygous single-nucleotide polymorphisms (SNPs) is a promising therapy for human trinucleotide repeat diseases such as Huntington's disease. Linking SNP identities to the two HTT alleles, normal and disease-causing, is a prerequisite for allele-specific RNA interference. Here we describe a method, SNP linkage by circularization (SLiC), to identify linkage between CAG repeat length and nucleotide identity of heterozygous SNPs using Huntington's disease patient peripheral blood samples.
authors.affiliation: Department of Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA.
authors.forename: Wanzhao
authors.initials: W
authors.lastname: Liu
authors.forename: Lori A
authors.initials: LA
authors.lastname: Kennington
authors.forename: H Diana
authors.initials: HD
authors.lastname: Rosas
authors.forename: Steven
authors.initials: S
authors.lastname: Hersch
authors.forename: Jang-Ho
authors.initials: JH
authors.lastname: Cha
authors.forename: Phillip D
authors.initials: PD
authors.lastname: Zamore
authors.forename: Neil
authors.initials: N
authors.lastname: Aronin
mesh_terms: D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018911:Trinucleotide Repeats / Q000235:genetics*