title: Kaposi's sarcoma-associated herpesvirus gH/gL: glycoprotein export and interaction with cellular receptors.
abstract: The attachment, entry, and fusion of Kaposi's sarcoma-associated herpesvirus (KSHV) with target cells are mediated by complex machinery containing, among others, viral glycoprotein H (gH) and its alleged chaperone, gL. We observed that KSHV gH, in contrast to its homologues in several other herpesviruses, is transported to the cytoplasm membrane independently from gL, but not vice versa. Mutational analysis revealed that the N terminus of gH is sufficient for gL interaction. However, the entire extracellular part of gH is required for efficient gL secretion. The soluble ectodomain of gH was sufficient to interact with the surfaces of potential target cells in a heparin-dependent manner, and binding was further enhanced by coexpression of gL. Surface plasmon resonance revealed a remarkably high affinity of gH for glycosaminoglycans. Heparan sulfate (HS) proteoglycans of the syndecan family act as cellular receptors for the gH/gL complex. They promoted KSHV infection, and expression of gH/gL on target cells inhibited subsequent KSHV infection. Whereas gH alone was able to bind to HS, we observed that only the gH/gL complex adhered to heparan sulfate-negative cells at lamellipodium-like structures.
authors.affiliation: Virologisches Institut, Universität Erlangen-Nürnberg, Schlossgarten 4, D-91054 Erlangen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Hahn
authors.forename: Alexander
authors.initials: A
authors.lastname: Birkmann
authors.forename: Effi
authors.initials: E
authors.lastname: Wies
authors.forename: Dominik
authors.initials: D
authors.lastname: Dorer
authors.forename: Kerstin
authors.initials: K
authors.lastname: Mahr
authors.forename: Michael
authors.initials: M
authors.lastname: Stürzl
authors.forename: Fritz
authors.initials: F
authors.lastname: Titgemeyer
authors.forename: Frank
authors.initials: F
authors.lastname: Neipel
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D004252:DNA Mutational Analysis; D006025:Glycosaminoglycans / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D019288:Herpesvirus 8, Human / Q000502:physiology*; D006801:Humans; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D021381:Protein Transport; D011991:Receptors, Virus / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C464461:glycoprotein H, human herpesvirus 8; C534114:glycoprotein L, human herpesvirus 8