title: Biosensor plates detect mitochondrial physiological regulators and mutations in vivo.
abstract: The measurement of mitochondrial activity in living cells is usually not straightforward, even though it is quite important in physiological and pathophysiological processes. We describe a high-throughput method for measurement of mitochondrial oxygen consumption in living cells, based on the Becton-Dickinson Biosensor plates.
authors.affiliation: Center for Neurosciences and Cell Biology, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Anabela P
authors.initials: AP
authors.lastname: Rolo
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Palmeira
authors.forename: Gino A
authors.initials: GA
authors.lastname: Cortopassi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D010101:Oxygen Consumption; D017354:Point Mutation / Q000235:genetics*; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D024101:Mitochondrial Proteins; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone