title: RNA bandages for photoregulating in vitro protein synthesis.
abstract: 'RNA bandages' are composed of two 6-12-mer 2'-OMe RNA strands complementary to a mRNA target that are joined by a photocleavable linker. These tandem oligonucleotides typically exhibit much higher affinity for the mRNA than the individual strands. An RNA bandage with binding arms of different lengths and a 4-base gap blocked translation in vitro of GFP mRNA; subsequent near-UV irradiation restored translation. This provides a general method of photomodulating hybridization for a variety of oligonucleotide-based technologies.
authors.affiliation: Department of Chemistry, University of Pennsylvania, 231 South 34th St., Philadelphia, PA 19104-6323, USA.
authors.forename: Julia L
authors.initials: JL
authors.lastname: Richards
authors.forename: XinJing
authors.initials: X
authors.lastname: Tang
authors.forename: Anna
authors.initials: A
authors.lastname: Turetsky
authors.forename: Ivan J
authors.initials: IJ
authors.lastname: Dmochowski
mesh_terms: D009693:Nucleic Acid Hybridization / Q000528:radiation effects; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry / Q000528:radiation effects*; D014176:Protein Biosynthesis / Q000187:drug effects; D012313:RNA / Q000737:chemistry* / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics; D012270:Ribosomes / Q000187:drug effects; D012689:Sequence Homology, Nucleic Acid
chemical_list: D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D012313:RNA