EphB1 is underexpressed in poorly differentiated colorectal cancers.
BACKGROUND
Over- or underexpression of certain Eph receptors has been associated with tumorigenesis of some types of cancer. EphB1 is a member of receptor tyrosine kinases of the EphB subfamily involved in the development, progress and prognosis of colorectal cancers. The expression levels of EphB1 in colon cancer cell lines and human colorectal carcinoma specimens were determined and association of EphB1 expression with clinicopathological parameters was analyzed.


METHODS
Quantitative real-time reverse transcription polymerase chain reaction and immunohistochemistry were used.


RESULTS
The EphB1 transcript is expressed in all colon cancer cell lines tested. However, there is marked variability in the expression of the EphB1 transcripts and proteins among colorectal carcinoma specimens. Reduced expression of EphB1 in colorectal cancers more often occurred in poorly differentiated and mucinous adenocarcinomas than in well- and moderately differentiated adenocarcinomas. Further, cancer cells with a low level of EphB1 protein showed more invasive power.


CONCLUSIONS
Our data indicate that EphB1 may have roles in the pathogenesis and development of colorectal cancer.
Sheng
Zhen
Z
Department of Pathology, Clinical School of Medical College of Nanjing University, Nanjing Jinling Hospital, Nanjing, PR China.
Wang
Jiandong
J
Dong
Yingchun
Y
Ma
Henghui
H
Zhou
Hangbo
H
Sugimura
Haruhiko
H
Lu
Guangming
G
Zhou
Xiaojun
X
D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D036225:Receptor, EphB1 / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D036225:Receptor, EphB1