title: Platelet lysate favours in vitro expansion of human bone marrow stromal cells for bone and cartilage engineering.
abstract: The heterogeneous population of non-haematopoietic cells residing in the bone marrow (bone marrow stromal cells, BMSCs) and the different fractions and components obtained from platelet-rich plasma provide an invaluable source of autologous cells and growth factors for bone and other connective tissue reconstruction. In this study, we investigated the effect of an allogenic platelet lysate on human BMSCs proliferation and differentiation. Cell proliferation and number of performed cell doublings were enhanced in cultures supplemented with the platelet-derived growth factors (platelet lysate, PL), either with or without the concomitant addition of fetal bovine serum (FBS), compared to cultures performed in the presence of FBS and FGF2. Both in vitro and in vivo osteogenic differentiation were unaltered in cells maintained in medium supplemented with PL and not FBS (Only PL) and in cells maintained in medium containing FBS and FGF2. Interestingly, the in vitro cartilage formation was more effective in the pellet of BMSCs expanded in the Only PL medium. In particular, a chondrogenic differentiation was observed in pellets of some in vitro-expanded BMSCs in the Only PL medium, whereas pellets from parallel cell cultures in medium containing FBS did not respond to the chondrogenic induction. We conclude that the platelet lysate from human source is an effective and even more beneficial substitute for fetal bovine serum to support the in vitro expansion of human BMSCs for subsequent tissue-engineering applications.
authors.affiliation: Istituto Nazionale per la Ricerca sul Cancro, Universita di Genova, Genova, Italy.
authors.forename: S H
authors.initials: SH
authors.lastname: Zaky
authors.forename: A
authors.initials: A
authors.lastname: Ottonello
authors.forename: P
authors.initials: P
authors.lastname: Strada
authors.forename: R
authors.initials: R
authors.lastname: Cancedda
authors.forename: M
authors.initials: M
authors.lastname: Mastrogiacomo
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology*; D001842:Bone and Bones / Q000378:metabolism*; D002356:Cartilage / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D020219:Chondrogenesis; D003114:Colony-Forming Units Assay; D005786:Gene Expression Regulation; D006801:Humans; D020007:Implants, Experimental; D051379:Mice; D010012:Osteogenesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling; D017154:Stromal Cells / Q000166:cytology*; D013997:Time Factors; D023822:Tissue Engineering; D054457:Tissue Scaffolds