Isolation and expression analysis of foxj1 and foxj1.2 in zebrafish embryos.
In this report, we present the isolation and identification of a zebrafish homolog of the winged helix\forkhead transcription factor Foxj1. Foxj1 was identified in other species but not in zebrafish. Foxj1 was shown in mice to be required in ciliogenesis and left-right asymmetry establishment. Here we present a spatio-temporal expression pattern of zebrafish foxj1, showing that this gene is expressed in dorsal forerunner cells, Kupffers vesicle, the floor plate, pronephric ducts and kidney. This expression pattern is overlapping but different from that of the foxj1.2, the closest related gene in zebrafish. Foxj1 in zebrafish appears to have similar functions as those reported in other species connected to ciliogenesis and left-right asymmetry.
Aamar
Emil
E
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Dawid
Igor B
IB
D000595:Amino Acid Sequence; D000818:Animals; D002923:Cilia / Q000378:metabolism; D004625:Embryo, Nonmammalian; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D017403:In Situ Hybridization; D055458:Left-Right Determination Factors; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D051858:Forkhead Transcription Factors; C533681:Foxj1a protein, zebrafish; C534385:Foxj1b protein, zebrafish; D055458:Left-Right Determination Factors; D029961:Zebrafish Proteins