Air pressure pulsation solid state fermentation of feruloyl esterase by Aspergillus niger.
Air pressure pulsation solid state fermentation (APP-SSF) was applied to produce feruloyl esterase (FAE) by Aspergillus niger. With the optimization of some variables by orthogonal design, the optimal condition obtained was 0.2 MPa (gauge pressure) of high pressure intensity, 30 min of low pressure duration and 20s of high pressure duration. Based on the optimized condition, the APP-SSF achieved the reasonable enzyme yield of 881 mU/g at 48 h, which was 58% more than that by static solid state fermentation (static SSF) at 72 h. By comparison of two fermentation methods in temperature, O(2) and CO(2) concentration, and respiration intensity, it was concluded that APP-SSF enhanced heat and mass transfer of fermentation system and strengthened the metabolism of microorganisms. The APP-SSF had a greatly positive effect on FAE production by A. niger, by enhancing mass and heat transfer and activating growth and metabolism.
Zeng
W
W
State Key Laboratory of Biochemistry, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
Chen
H Z
HZ
D000399:Air Pressure; D001234:Aspergillus niger / Q000201:enzymology*; D019149:Bioreactors / Q000382:microbiology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D009991:Oscillometry / Q000379:methods; D044367:Phase Transition; D011786:Quality Control
D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase