Peptide-based SAMs that resist the adsorption of proteins.
In this paper we present a modular approach for the fabrication of surfaces to characterize protein-protein interactions. The approach is based on azido peptides with an optimized sequence which are then thiol-functionalized using an alkynyl thiol and "click" chemistry. From these peptide thiols we fabricated SAMs on gold to evaluate the protein resistance, using surface plasmon resonance spectroscopy, toward streptavidin, bovin serum albumin (BSA), and fibronectin.
Chelmowski
Rolf
R
Physikalische Chemie 1 and Anorganische Chemie I, Ruhr-Universität Bochum, 44780 Bochum, Germany.
Köster
Stephan David
SD
Kerstan
Andreas
A
Prekelt
Andreas
A
Grunwald
Christian
C
Winkler
Tobias
T
Metzler-Nolte
Nils
N
Terfort
Andreas
A
Wöll
Christof
C
D000327:Adsorption; D000595:Amino Acid Sequence; D001386:Azides / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D001386:Azides; D001672:Biocompatible Materials; D005353:Fibronectins; D010455:Peptides; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D019809:Streptavidin