title: Acidophilic haloarchaeal strains are isolated from various solar salts.
abstract: Haloarchaeal strains require high concentrations of NaCl for their growth, with optimum concentrations of 10-30%. They display a wide variety of morphology and physiology including pH range for growth. Many strains grow at neutral to slightly alkaline pH, and some only at alkaline pH. However, no strain has been reported to grow only in acidic pH conditions within the family Halobacteriaceae.In this study, we isolated many halophiles capable of growth in a 20% NaCl medium adjusted to pH 4.5 from 28 commercially available salts. They showed growth at pH 4.0 to 6.5, depending slightly on the magnesium content. The most acidophilic strain MH1-52-1 isolated from an imported solar salt (pH of saturated solution was 9.0) was non-pigmented and extremely halophilic. It was only capable of growing at pH 4.2-4.8 with an optimum at pH 4.4 in a medium with 0.1% magnesium chloride, and at pH 4.0-6.0 (optimum at pH 4.0) in a medium with 5.0% magnesium. The 16S rRNA and DNA-dependent RNA polymerase subunit B' gene sequences demonstrated clearly that the strain MH1-52-1 represents a new genus in the family Halobacteriaceae.
authors.affiliation: Bio-Nano Electronics Research Center, Toyo University, Kawagoe, Saitama, Japan. minehiro@toyonet.toyo.ac.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Minegishi
authors.forename: Toru
authors.initials: T
authors.lastname: Mizuki
authors.forename: Akinobu
authors.initials: A
authors.lastname: Echigo
authors.forename: Tadamasa
authors.initials: T
authors.lastname: Fukushima
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kamekura
authors.forename: Ron
authors.initials: R
authors.lastname: Usami