Trans-species activation of human T cells by rhesus macaque CD1b molecules.
Despite crucial importance of non-human primates as a model of human infectious diseases, group 1 CD1 genes and proteins have been poorly characterized in these species. Here, we isolated CD1A, CD1B, and CD1C cDNAs from rhesus macaque lymph nodes that encoded full-length CD1 proteins recognized specifically by monoclonal antibodies to human CD1a, CD1b, and CD1c molecules, respectively. The monkey group 1 CD1 isoforms contained amino acid residues and motifs known to be critical for intramolecular disulfide bond formation, N-linked glycosylation, and endosomal trafficking as in human group 1 CD1 molecules. Notably, monkey CD1b molecules were capable of presenting a mycobacterial glycolipid to human CD1b-restricted T cells, providing direct evidence for their antigen presentation function. This also detects for the first time a trans-species crossreaction mediated by group 1 CD1 molecules. Taken together, these results underscore substantial conservation of the group 1 CD1 system between humans and rhesus macaque monkeys.
Morita
Daisuke
D
Laboratory of Cell Regulation, Institute for Virus Research, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Katoh
Kumiko
K
Harada
Toshiyuki
T
Nakagawa
Yoshiaki
Y
Matsunaga
Isamu
I
Miura
Tomoyuki
T
Adachi
Akio
A
Igarashi
Tatsuhiko
T
Sugita
Masahiko
M
D020816:Amino Acid Motifs; D000818:Animals; D017951:Antigen Presentation; D018949:Antigens, CD1 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D003429:Cross Reactions; D018360:Crystallography, X-Ray; D018076:DNA, Complementary / Q000235:genetics / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008253:Macaca mulatta / Q000235:genetics / Q000276:immunology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000276:immunology*
D018949:Antigens, CD1; C419393:CD1a antigen; C419394:CD1b antigen; D018076:DNA, Complementary