[Genetic types of three community-acquired methicillin-resistant Staphylococcus aureus isolates].
OBJECTIVE
To investigate the genetic features of the community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) prevalent in West China.


METHODS
Three CA-MRSA isolates obtained in Chengdu, Sichuan, underwent SCCmec (Staphylococcal Cassette Chromosome mec) multi-PCR, Staphylococcal protein A (spa) typing and multi-locus sequence typing (MLST) method, and their Panton-Valentine leucocidin (PVL) gene was also detected.


RESULTS
All 3 CA-MRSA isolates were positive of mecA gene (147 bp), and the other PCR product of 750 bp was confirmed to be type IVa SCCmec. Spa typing showed that the MRSA strains s29635 and s35301 were typed as t437, and the strain s19165 was typed as t008. MLST showed that the MRSA strains s29635 and s35301 were typed as ST59, and the strain s19165 was typed as ST8. The strains s19165 and s35301 were all positive for PVL gene, and the strain s29635 was negative for PVL gene.


CONCLUSIONS
CA-MRSA clones ST8-t008 and ST59-t437 have been isolated in West China.
Li
Nan
N
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Fan
Hong
H
Chen
Hui-li
HL
Ye
Yuan-xin
YX
Lu
Xiao-jun
XJ
Xie
Yi
Y
Ying
Bin-wu
BW
D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D002681:China; D017714:Community-Acquired Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007297:Inpatients; D008297:Male; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology; D013205:Staphylococcal Protein A / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D013205:Staphylococcal Protein A