Influence of dietary phytic acid and source of microbial phytase on ileal endogenous amino acid flows in broiler chickens.
The effects of phytic acid and 2 sources of exogenous phytase (bacterial vs. fungal) on the flow of endogenous amino acids at the terminal ileum of broilers were assessed using the enzyme-hydrolyzed casein method. Phytic acid (as the sodium salt) was included in a purified diet at 8.5 and 14.5 g/kg, and each diet was fed without or with a fungal (Aspergillus niger-derived) or a bacterial (Escherichia coli-derived) microbial phytase at 500 phytase units/kg of diet. Increasing the concentration of phytic acid in the diet from 8.5 to 14.5 g/kg increased (P < 0.001) the flow of all measured amino acids by an average of 68%, with a range from 17% for proline to 145% for phenylalanine. The flow of endogenous aspartic acid, serine, glutamic acid, glycine, leucine, tyrosine, phenylalanine, and histidine were increased by more than the mean, indicating changes in the composition of endogenous protein in response to the presence of higher concentrations of phytic acid. Supplementation of both phytases reduced (P < 0.001) the flow of endogenous amino acids, but the reduction (P = 0.06) was greater for the bacterial phytase compared with the fungal phytase. These data suggest that a substantial part of the amino acid and energy responses observed following phytase supplementation in broiler chickens stems from reduced endogenous amino acid flows and that the capacity of different phytases to counteract the antinutritive properties of phytic acid vary.
Cowieson
A J
AJ
Danisco Animal Nutrition, Marlborough, Wiltshire, SN8 1XN, UK.
Ravindran
V
V
Selle
P H
PH
D010832:6-Phytase / Q000378:metabolism*; D000284:Administration, Oral; D000596:Amino Acids / Q000378:metabolism*; D000821:Animal Feed; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology / Q000378:metabolism; D002645:Chickens; D004063:Digestion; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D005163:Factor Analysis, Statistical; D005612:Freeze Drying; D005656:Fungal Proteins / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010833:Phytic Acid / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*
D000596:Amino Acids; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D010833:Phytic Acid; D010832:6-Phytase