title: Simultaneous enantioseparation of four beta2-agonists by capillary electrophoresis with cyclodextrin additives. Study of the enantioselective mechanism.
abstract: The simultaneous capillary electrophoretic enantioseparation of adrenergic beta(2)-agonists enantiomers (trantinterol, mabuterol, clenbuterol, bambuterol) was studied with beta-cyclodextrin, ethyl-beta-CD, methyl-beta-CD, hydroxypropyl-beta-CD, and hydroxyethyl-beta-CD as chiral selector. The type and concentration of the chiral selector and buffer pH played a very important role in the enantioseparation of the analyzed compounds. Hydroxypropyl-beta-CD was found to be the most effective complexing agent and allowed excellent chiral/achiral resolutions compared to the other CDs. The simultaneous enantioseparation of four beta(2)-agonists was achieved using 100 mM citric acid-10 mM Na(2)HPO(4) buffer at pH 2.5 containing 120 mM hydroxypropyl-beta-CD with an applied voltage of 20 kV. Method validation in terms of repeatability, linearity, and limits of detection and quantification was performed. The effect of structural features of analytes on R(s) and t(m) was studied. Complexation binding constants for the interactions between the four compounds and three different CDs were evaluated for elucidating the enantioseparation mechanism. It was found that very small differences in the chemical structure of the analytes resulted in significant changes in stereoselective recognition.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Xiumei
authors.initials: X
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.forename: Kun
authors.initials: K
authors.lastname: Jiang
authors.forename: Maosheng
authors.initials: M
authors.lastname: Cheng
authors.forename: Famei
authors.initials: F
authors.lastname: Li
mesh_terms: D000318:Adrenergic beta-Agonists / Q000302:isolation & purification*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D015203:Reproducibility of Results; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000318:Adrenergic beta-Agonists; D002021:Buffers; D047392:beta-Cyclodextrins