[Isolation and characterization of Thermoanaerobacter mathranii SC-2 from oil-field water].
OBJECTIVE
We studied physiological, biochemical properties and metabolites of Thermoanaerobacter mathranii SC-2 from oil-field water in Shengli oilfield.


METHODS
Strain SC-2 was isolated by Hungate anaerobic technique. Through physiological, biochemical and phylogenetic analysis, the strain was identified. Metabolites were analyzed by gas chromatogram.


RESULTS
The cells were Gram-negative, rod-shaped, spore-forming. Growth was observed in the temperature range from 40 to 75degrees C (optimum 70 degrees C) and pH range from 5.5 to 9.5 (optimum 6.5). The isolate grew in the presence of 0%-5% NaCl with an optimum without NaCl at pH 7.0 and 65 degrees C. Strain SC-2 used many carbohydrates as carbon sources, including glucose and xylose. Metabolites of glucose were ethanol, acetate, propionate, lactate, CO2 and H2. Based on 16S rDNA studies, strain SC-2 was most close to T. mathranii subsp. mathranii11246T with 99.85% similarity. More ethanol and acetate were produced at initial pH 8.0 than yields at other pH. Yeast extract could significantly increase ethanol and acetate yields. In addition, ethanol (4%) added in the medium obviously inhibited its growth.


CONCLUSIONS
Strain SC-2 was extremely thermophilic, halotolerant anaerobe.
Li
Xia
X
Biogas Institute of Ministry of Agriculture, Chengdu 610041, China. xiaxiali@yahoo.com.cn
Cheng
Lei
L
Wang
Weidong
W
Deng
Yu
Y
Yin
Xiaobo
X
Zhang
Hui
H
D000085:Acetates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D000431:Ethanol / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D008854:Microscopy, Electron; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D011422:Propionates / Q000378:metabolism; D013696:Temperature; D046968:Thermoanaerobacter / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism* / Q000648:ultrastructure; D014871:Water Microbiology
D000085:Acetates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007773:Lactates; D010578:Petroleum; D011422:Propionates; D002245:Carbon Dioxide; D000431:Ethanol; D006859:Hydrogen