title: An avian live attenuated master backbone for potential use in epidemic and pandemic influenza vaccines.
abstract: The unprecedented emergence in Asia of multiple avian influenza virus (AIV) subtypes with a broad host range poses a major challenge in the design of vaccination strategies that are both effective and available in a timely manner. The present study focused on the protective effects of a genetically modified AIV as a source for the preparation of vaccines for epidemic and pandemic influenza. It has previously been demonstrated that a live attenuated AIV based on the internal backbone of influenza A/Guinea fowl/Hong Kong/WF10/99 (H9N2), called WF10att, is effective at protecting poultry species against low- and high-pathogenicity influenza strains. More importantly, this live attenuated virus provided effective protection when administered in ovo. In order to characterize the WF10att backbone further for use in epidemic and pandemic influenza vaccines, this study evaluated its protective effects in mice. Intranasal inoculation of modified attenuated viruses in mice provided adequate protective immunity against homologous lethal challenges with both the wild-type influenza A/WSN/33 (H1N1) and A/Vietnam/1203/04 (H5N1) viruses. Adequate heterotypic immunity was also observed in mice vaccinated with modified attenuated viruses carrying H7N2 surface proteins. The results presented in this report suggest that the internal genes of a genetically modified AIV confer similar protection in a mouse model and thus could be used as a master donor strain for the generation of live attenuated vaccines for epidemic and pandemic influenza.
authors.affiliation: Department of Veterinary Medicine, University of Maryland, College Park and Virginia-Maryland Regional College of Veterinary Medicine, 8075 Greenmead Drive, College Park, MD 20742-3711, USA.
authors.forename: Danielle
authors.initials: D
authors.lastname: Hickman
authors.affiliation: Department of Veterinary Medicine, University of Maryland, College Park and Virginia-Maryland Regional College of Veterinary Medicine, 8075 Greenmead Drive, College Park, MD 20742-3711, USA.
authors.forename: Md Jaber
authors.initials: MJ
authors.lastname: Hossain
authors.affiliation: Department of Veterinary Medicine, University of Maryland, College Park and Virginia-Maryland Regional College of Veterinary Medicine, 8075 Greenmead Drive, College Park, MD 20742-3711, USA.
authors.forename: Haichen
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Veterinary Medicine, University of Maryland, College Park and Virginia-Maryland Regional College of Veterinary Medicine, 8075 Greenmead Drive, College Park, MD 20742-3711, USA.
authors.forename: Yonas
authors.initials: Y
authors.lastname: Araya
authors.affiliation: Department of Veterinary Medicine, University of Maryland, College Park and Virginia-Maryland Regional College of Veterinary Medicine, 8075 Greenmead Drive, College Park, MD 20742-3711, USA.
authors.forename: Alicia
authors.initials: A
authors.lastname: Solórzano
authors.affiliation: Department of Veterinary Medicine, University of Maryland, College Park and Virginia-Maryland Regional College of Veterinary Medicine, 8075 Greenmead Drive, College Park, MD 20742-3711, USA.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Perez
mesh_terms: D000328:Adult; D000818:Animals; D001717:Birds; D002460:Cell Line; D002648:Child; D004196:Disease Outbreaks / Q000517:prevention & control* / Q000662:veterinary*; D004285:Dogs; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000627:therapeutic use*; D005585:Influenza in Birds / Q000453:epidemiology / Q000276:immunology*; D007251:Influenza, Human / Q000453:epidemiology* / Q000276:immunology / Q000401:mortality / Q000821:virology; D007668:Kidney / Q000196:embryology; D009500:Neutralization Tests; D014613:Vaccines, Attenuated / Q000627:therapeutic use*; D014614:Vaccines, Synthetic / Q000276:immunology; D019562:Viral Load; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D014764:Viral Proteins