Warm water induces apoptosis, gonadal degeneration, and germ cell loss in subadult pejerrey Odontesthes bonariensis (Pisces, Atheriniformes).
Abstract Recent studies have shown that exposure to warm water can trigger gonadal degeneration and germ cell loss in fish of both sexes, but the mechanism behind this pathology is still not understood. This study was designed to characterize this process histologically and determine whether apoptosis plays any role during high temperature-induced gonadal cell degeneration in subadult pejerrey (Odontesthes bonariensis). For this purpose, fish were reared continuously at constant temperatures of 24 degrees C (control) and 29 degrees C (prolonged heat stress) or exposed for 36 h to 31 degrees C and then returned to 24 degrees C (short heat stress). Gonads were sampled at various times (hours, days, weeks) after the start of the experiment and were analyzed by light microscopy and stereometry for histological integrity/degeneration and germ cell counts, as well as by acridine orange fluorescence microscopy, TUNEL, and caspase activity assay for histochemical and biochemical signs of apoptosis. The results clearly implicate apoptosis in heat-induced somatic and germ cell degeneration in pejerrey and revealed that the dynamics and severity of this process were proportional to the magnitude of the thermal stress. Even a 36-h exposure to 31 degrees C induced significant increases in caspase-3 activity and number of apoptotic cells in both sexes, but males were shown to be more sensitive to heat stress than females.
Ito
Lauro Satoru
LS
Department of Marine Biosciences, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
Takahashi
Chika
C
Yamashita
Michiaki
M
Strüssmann
Carlos Augusto
CA
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D005260:Female; D005399:Fishes / Q000502:physiology*; D005854:Germ Cells / Q000166:cytology / Q000502:physiology; D006358:Hot Temperature / Q000009:adverse effects*; D008297:Male; D010053:Ovary / Q000166:cytology / Q000254:growth & development*; D012741:Sexual Maturation / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000254:growth & development*; D014867:Water
D014867:Water