[Expression variation and significance of Skp2 and p27(kip1) during the proliferation of Jurkat cells].
OBJECTIVE
To investigate the expression variation and significance of Skp2 and p27(kip1) during the proliferation of lymphoma cell line Jurkat cells.


METHODS
The binding of p27(kip1) and Skp2 in Jurkat cells were detected by immunoprecipitation. Jurkat cells were treated with serum starvation and release synchronization. The expression variation and subcellular localization of p27(kip1) and Skp2 were detected by subcellular fractionation, Western blot and double immunofluorescence labelling.


RESULTS
The results of immunoprecipitation suggested that p27(kip1) and Skp2 could bind each other in Jurkat cells. During the proliferation of Jurkat cells, the protein expression of p27(kip1) decreased and intranuclear p27(kip1) decreased significantly, while the Skp2 protein increased and cytoplasmic Skp2 increased significantly.


CONCLUSIONS
During the proliferation of Jurkat cells, the increased cytoplasmic synthesis of Skp2 may speed up p27(kip1) degradation via the ubiquitin-proteasome pathway, then intranuclear p27(kip1) decreases significantly, leading to an increased cell cycling activity.
Lu
Jian-Xin
JX
Department of Microbiology and Immunology, Nantong University, Nantong 226001, China.
Wang
Yu-Chan
YC
Shen
Ai-Guo
AG
Zhao
Yue-Ming
YM
Sun
Cheng-Long
CL
Zhang
Dong-Mei
DM
Cheng
Chun
C
D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D016393:Lymphoma, B-Cell / Q000378:metabolism / Q000473:pathology; D011485:Protein Binding; D044786:S-Phase Kinase-Associated Proteins / Q000378:metabolism*
D044786:S-Phase Kinase-Associated Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27