[Effects of intravenous injection of sulphated galactomannan on anticoagulant activity of rat plasma].
We studied anticoagulant activity in vitro and in vivo of sulfate depolymerisation galactomannan guar with the following characteristics: Man:Gal 1.64, molecular mass 127 kDa, sulfation degree 1.46. We found the ability of galactomannan-HSO3Na (GM) to increase the time of blood coagulation in the test aPTT with an increase of its concentration and to decrease velocity of chromogenic substrate on the factor Xa hydrolysis. Specific antithrombin and anti-factor Xa activities of GM were 35.8 +/- 1.8 IU/mg and 6.6 +/- 0.5 IU/mg, respectively. In biospecific electrophoresis complexes arise between GM and protamine sulfate. Intravenous injection of GM to rats prolonged plasma coagulation time in the aPTT test with dose reduction from 1 to 3 mg/kg. In rat thrombosis model a dose of 3 Mg/kg produced a 100% inhibition of blood clot.
Drozd
N N
NN
Makarov
V A
VA
Tolstenkov
A S
AS
Lapikova
E S
ES
Miftakhova
N T
NT
Mestechkina
N M
NM
Shcherbukhin
V D
VD
Il'ina
A V
AV
Varlamov
V P
VP
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D004195:Disease Models, Animal; D015951:Factor Xa / Q000378:metabolism*; D005690:Galactose / Q000031:analogs & derivatives; D006868:Hydrolysis; D008297:Male; D008351:Mannans / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013927:Thrombosis / Q000188:drug therapy* / Q000378:metabolism
D000925:Anticoagulants; D008351:Mannans; C012990:galactomannan; D015951:Factor Xa; D005690:Galactose