Paclitaxel succinate analogs: Anionic and amide introduction as a strategy to impart blood-brain barrier permeability.
A focused library of TX-67 (C10 hemi-succinate) analogs has been prepared, including C7 regioisomers, esters, amides, and one-carbon homologs. These were prepared to investigate whether the lack of TX-67 interaction with P-glycoprotein (Pgp) is due to the presence of the carboxylic acid moiety and whether this phenomenon was restricted to C10 analogs. Tubulin stabilization ability, cytotoxicity, and Pgp interactions were evaluated. All carboxylic acid analogs and several of the amides had no apparent interactions with Pgp at the concentrations used, whereas the ester variants displayed characteristics of Pgp substrates. Furthermore, it was demonstrated that hydrogen-bonding properties were significant with respect to Pgp interactions. Calculations of logD and cross-sectional areas revealed that these analogs are predicted to partition into the membrane and can compete for Pgp binding sites. The anionic and amide introduction strategy may allow for delivery of paclitaxel into the CNS and may be a potential approach for the delivery of other, structurally complex and lipophilic non-CNS permeable drugs.
Turunen
Brandon J
BJ
Department of Medicinal Chemistry, University of Kansas, Malott Hall 4070, 1251 Wescoe Hall Drive, Lawrence, KS 66045, USA.
Ge
Haibo
H
Oyetunji
Jariat
J
Desino
Kelly E
KE
Vasandani
Veena
V
Güthe
Sarah
S
Himes
Richard H
RH
Audus
Kenneth L
KL
Seelig
Anna
A
Georg
Gunda I
GI
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002490:Central Nervous System / Q000187:drug effects; D020650:Combinatorial Chemistry Techniques; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D015394:Molecular Structure; D017239:Paclitaxel / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D013237:Stereoisomerism; D013386:Succinates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D050257:Tubulin Modulators / Q000494:pharmacology
C497149:10-O-deacetylpaclitaxel 10-monosuccinyl ester; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D013386:Succinates; D050257:Tubulin Modulators; D017239:Paclitaxel