Stimulation of endometrium embryo transfer can improve implantation and pregnancy rates for patients undergoing assisted reproductive technology for the first time with a high-grade blastocyst.
OBJECTIVE
To evaluate whether stimulation of endometrium embryo transfer (SEET) can improve implantation rate and pregnancy rate (PR) for patients undergoing assisted reproductive technology (ART) for the first time by injecting embryo culture supernatant into the uterus before blastocyst transfer (BT).


METHODS
Randomized, controlled trial.


METHODS
Private in vitro fertilization clinic.


METHODS
Forty-eight women in the BT group, 48 women in the stimulation group who had culture medium injected into the uterus before BT, and 48 women in the SEET group.


METHODS
Injection of embryo culture supernatant and injection of culture medium.


METHODS
Implantation rates and PRs.


RESULTS
Odds ratios of successful implantation rate for stimulation and SEET in patients with high-grade blastocysts, having BT as reference, were 2.58 and 6.46 without adjustment, and 5.91 and 9.20 after adjusting for basal FSH levels and period of infertility. Odds ratios of clinical pregnancies were 2.47 and 4.32 without adjustment, and 4.46 and 5.10 with adjustment, respectively. In groups with low-grade blastocysts, such tendencies were not observed.


CONCLUSIONS
The SEET may be an effective method for increasing implantation rate and PR for first-time ART patients who have a high-grade blastocyst.
Goto
Sakae
S
Hanabusa Women's Clinic, Kobe, Japan. Electronic address: gotoms@tcn.zaq.ne.jp.
Kadowaki
Takashi
T
Department of Health Science, Shiga University of Medical Science, Otsu, Japan.
Hashimoto
Hiromi
H
Hanabusa Women's Clinic, Kobe, Japan.
Kokeguchi
Shoji
S
Hanabusa Women's Clinic, Kobe, Japan.
Shiotani
Masahide
M
Hanabusa Women's Clinic, Kobe, Japan.
D000328:Adult; D001755:Blastocyst / Q000166:cytology; D003470:Culture Media / Q000494:pharmacology*; D046149:Embryo Culture Techniques; D010064:Embryo Implantation / Q000187:drug effects / Q000502:physiology; D004624:Embryo Transfer / Q000379:methods*; D004717:Endometrium / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000503:physiopathology / Q000628:therapy; D011247:Pregnancy; D018873:Pregnancy Rate; D011786:Quality Control; D027724:Reproductive Techniques, Assisted; D013268:Stimulation, Chemical; D016896:Treatment Outcome
D003470:Culture Media