Isolation and identification of a novel cis-epoxysuccinate hydrolase-producing Bordetella sp. BK-52 and optimization of enzyme production.
OBJECTIVE
To isolate and identify a novel strain with cis-epoxysuccinate hydrolase (CESH) activity and to optimize its enzyme production.


METHODS
The isolated strain was identified by electron microscopy, Biolog gram negative (GN) test, G+C (guanine plus cytosine) content measurement and 16S rDNA sequence. The purified enzymatic biotransformed product was identified by IR, 1H-NMR, 13C-NMR, MS and optical rotation analysis. Then the fermentation conditions for CESH production were optimized.


RESULTS
A novel CESH-producing strain was isolated for biotransforming cis-epoxysuccinate to D(-)-tartaric acid. It was assigned to genus Bordetella and named Bordetella sp. BK-52. The optimal conditions were found to be 30 degrees C, pH 7.0, fermentation time 36 h, carbon source of saccharose, inorganic nitrogen source of ammonium sulfate and enzyme inducer of disodium cis-epoxysuccinate. Under these conditions, the maximum CESH activity reached 764 U/g biomass.


CONCLUSIONS
The isolated Bordetella sp. BK-52 provided a new alternative for biosynthesis of D(-)-tartaric acid from cis-epoxysuccinate.
Pan
Haifeng
H
Institute of Biochemistry, Zhejiang University, Hangzhou 310058, China. panlook@163.com
Xie
Zhipeng
Z
Bao
Wenna
W
Zhang
Jianguo
J
D018533:Biomass; D001884:Bordetella / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism* / Q000648:ultrastructure; D002244:Carbon / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000096:biosynthesis*; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D017672:Nitrogen Compounds / Q000494:pharmacology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D017672:Nitrogen Compounds; D002244:Carbon; D006867:Hydrolases