title: Validation of high-affinity binding sites for succinic acid through distinguishable binding of gamma-hydroxybutyric acid receptor-specific NCS 382 antipodes.
abstract: Gamma-hydroxybutyric acid (GHB) binding to multiple sites for the tricarboxylic acid cycle intermediate succinic acid (SUC) has been disclosed recently. In order to better characterize these targets, distinguishable binding of GHB receptor-specific NCS 382 antipodes to [(3)H]-SUC or [(3)H]-GHB labelled sites in rat brain synaptic membranes was explored. Eutomer binding parameters suggest identity of the high-affinity target for SUC with a synaptic GHB receptor subtype.
authors.affiliation: Department of Neurochemistry, Chemical Research Center, Hungarian Academy of Sciences, Budapest, Hungary. tmolnar@chemres.hu
authors.forename: Tünde
authors.initials: T
authors.lastname: Molnár
authors.forename: Júlia
authors.initials: J
authors.lastname: Visy
authors.forename: Agnes
authors.initials: A
authors.lastname: Simon
authors.forename: István
authors.initials: I
authors.lastname: Moldvai
authors.forename: Eszter
authors.initials: E
authors.lastname: Temesvári-Major
authors.forename: Gábor
authors.initials: G
authors.lastname: Dörnyei
authors.forename: Erzsébet Kútiné
authors.initials: EK
authors.lastname: Fekete
authors.forename: Julianna
authors.initials: J
authors.lastname: Kardos
mesh_terms: D000818:Animals; D001567:Benzocycloheptenes / Q000494:pharmacology*; D001665:Binding Sites; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D006885:Hydroxybutyrates / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013237:Stereoisomerism; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: C039403:4-hydroxybutyric acid receptor; D001567:Benzocycloheptenes; D006885:Hydroxybutyrates; D011956:Receptors, Cell Surface; C066471:NCS 382; C111420:4-hydroxybutyric acid; D019802:Succinic Acid