[Screen and identification of genes involved in multidrug resistance in Pseudomonas aeruginosa].
OBJECTIVE
To identify genes involved in multidrug resistance in Pseudomonas aeruginosa.


METHODS
We constructed and screened a transposon mutation library and obtained mutants that exhibited decreased drug resistance. The insertion sites of the transposon were identified by arbitrary PCR and subsequent DNA sequencing.


RESULTS
Two mutants which became more susceptible to several antibacterial agents than the wild type were identified as mutants with transposon insertion in gene PA2580 and PA2800 respectively. The functions of these two genes are described as unknown in P. aeruginosa database.


CONCLUSIONS
PA2580 and PA2800 are involved in drug resistance, possibly through their roles in redox reactions and cell envelope biogenesis.
Zhao
Xingyan
X
Molecular Microbiology Laboratory, Northwest University, Xi,an 710069, China. zxyyt0801@163.com
Liang
Haihua
H
Shen
Lixin
L
Dong
Zhaolin
Z
Duan
Kangmin
K
D000595:Amino Acid Sequence; D004251:DNA Transposable Elements / Q000235:genetics; D030541:Databases, Genetic; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics*
D004251:DNA Transposable Elements