title: Quercetin and interferon-beta modulate immune response(s) in peripheral blood mononuclear cells isolated from multiple sclerosis patients.
abstract: The study is aimed to determine the role of quercetin (3,3'4',5,7-pentahydroxy flavone), alone and in combination with human interferon-beta (IFN-beta), in modulating the immune response(s) of peripheral blood mononuclear cells (PBMC) isolated from multiple sclerosis (MS) patients and from normal healthy subjects. PBMC proliferation in the presence or absence of these drugs was determined and the production of proinflammatory cytokines (IL-1beta, TNF-alpha), and the ratio of cell migration mediator MMP-9, and its inhibitor, TIMP-1 were assessed in the culture supernatants. Quercetin reduced, in a dose-dependent manner, the proliferation of PBMC and modulated the level of IL-1beta and TNF-alpha released by PBMC in the culture supernatants. Quercetin reduced the MMP-9/TIMP-1 ratio via lowering MMP-9 production. Quercetin, when combined with IFN-beta, had additive effects in modulating TNF-alpha and MMP-9. These immunomodulatory responses to quercetin were similar between MS patients and healthy control (HC) subjects.
authors.affiliation: Department of Neurology, Baird MS Center, Jacobs Neurological Institute, Buffalo, NY 14223 , United States. zs2@buffalo.edu
authors.forename: Zohara
authors.initials: Z
authors.lastname: Sternberg
authors.forename: Kailash
authors.initials: K
authors.lastname: Chadha
authors.forename: Alicia
authors.initials: A
authors.lastname: Lieberman
authors.forename: David
authors.initials: D
authors.lastname: Hojnacki
authors.forename: Allison
authors.initials: A
authors.lastname: Drake
authors.forename: Paolo
authors.initials: P
authors.lastname: Zamboni
authors.forename: Paluch
authors.initials: P
authors.lastname: Rocco
authors.forename: Erica
authors.initials: E
authors.lastname: Grazioli
authors.forename: Bianca
authors.initials: B
authors.lastname: Weinstock-Guttman
authors.forename: Frederick
authors.initials: F
authors.lastname: Munschauer
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D016022:Case-Control Studies; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D016899:Interferon-beta / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology* / Q000473:pathology; D011794:Quercetin / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D016207:Cytokines; D007155:Immunologic Factors; D016899:Interferon-beta; D011794:Quercetin; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1