Bone mesenchymal stem cells for gene transfer of NGF to the adult rat brain: rescue the NGFR p75 positive neurons from fimbria-fornix lesion-induced degeneration.
The study was to evaluate the therapeutic benefit of transplanted bone mesenchymal stem cells (BMSCs) transfected never growth factor (NGF) gene and GFP gene (as a reporter gene), in treating the rat with fimbria-fornix lesion. After transduction of NGF gene via recombinant retroviral vectors into the rat BMSCs, BMSCs were therefore transformed into the GFP-NGF positive BMSCs, nearly 100% of BMSCs expressed NGF, and then transplanted into basal forebrain of rat with fimbria-fornix lesion. After 2 weeks post-transplantation, the GFP-NGF positive BMSCs survive and fuse in vivo with astroglia or NGFR p75 positive neurons in the basal forebrain, no evidence of transdifferentiation was observed in this study. The number of NGFR p75 positive neurons in basal forebrain of NGF group was significantly higher than those of the void plasmid group (p < 0.05) or the PBS group (p < 0.01). These results indicate that the GFP-NGF positive BMSCs provide, by way of paracrine, NGF that effectively perform the functions of neuroprotection, which cell fusion may be also contribute to.
Leng
Shuilong
S
Department of Oral Anatomy and Physiology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510080, PR China.
He
Junfeng
J
Fan
Wenguo
W
Cheng
Shaowu
S
Long
Dahong
D
He
Hongwen
H
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D001842:Bone and Bones / Q000166:cytology*; D001921:Brain / Q000502:physiology*; D001923:Brain Chemistry; D005260:Female; D005455:Fluorescent Antibody Technique; D020712:Fornix, Brain / Q000293:injuries*; D018014:Gene Transfer Techniques; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009410:Nerve Degeneration / Q000235:genetics* / Q000517:prevention & control*; D020932:Nerve Growth Factor / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020800:Receptor, Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
D020800:Receptor, Nerve Growth Factor; D020932:Nerve Growth Factor