[Application of molecular method in identification of bacterial endophthalmitis].
OBJECTIVE
To search for a non-culture method to improve the efficiency of etiological diagnosis of infective endophthalmitis and other infectious diseases.


METHODS
Fifty clinical specimens of infective endophthalmitis were collected. DNA of bacterium was extracted directly from the aqueous fluid or vitreous fluid and then amplified with universal primers. The PCR products were sequenced subsequently.


RESULTS
Twenty-two (44%) bacterial smears and 13 (26%) cultured specimens were positive. PCR positivity was seen in 28 of the 50 cases (56%) and 22 specimens (44%) were sequenced successfully. The results of 16s rRNA gene sequence analysis did not completely conform to the results obtained from culture-dependent approach.


CONCLUSIONS
Molecular identification method is a rapid and efficient approach for the diagnosis of bacterial infective endophthalmitis.
Wu
Wei
W
Department of Clinical Laboratory, Beijing Tongren Hospital Affiliated to Capital University of Medical Sciences, Beijing 100730, China.
Lu
Xin-xin
XX
Huang
Yan-fei
YF
D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D009877:Endophthalmitis / Q000175:diagnosis* / Q000382:microbiology; D015818:Eye Infections, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014822:Vitreous Body / Q000382:microbiology
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S