Isolation of the constitutive heterochromatin from mouse liver nuclei.
A method for isolation of constitutive heterochromatin (chromocenters) from nuclei of mouse liver cells is described. This method is based on the higher resistance of chromocenters to low ionic strength treatment as compared with that of nucleoli and euchromatin. The method allows separation of chromocenters that are essentially free of nucleoli and other nuclear contaminants. In contrast to nuclei and nucleoli, isolated chromocenters are characterized by a simpler protein composition and contain a smaller number of proteins (especially of high molecular weight proteins). They possess telomeric DNA and telomerase activity that suggests a tight association of chromocenters with the telomerase complex in mouse hepatocyte nuclei.
Zatsepina
Olga V
OV
A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
Zharskaya
Oxana O
OO
Prusov
Andrei N
AN
D000818:Animals; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism; D022041:Euchromatin / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology; D006570:Heterochromatin / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000379:methods; D008954:Models, Biological; D008967:Molecular Biology / Q000379:methods*; D016615:Telomere / Q000648:ultrastructure
D022041:Euchromatin; D006570:Heterochromatin; D004247:DNA