Genetic diversity of Streptomyces spp. causing common scab of potato in eastern Canada.
Common scab is an important disease of potato caused by Streptomyces scabies and other closely related species. In this study, the genetic diversity of Streptomyces spp. causing common scab of potato in eastern Canada was for the first time investigated. Forty-one Streptomyces spp. isolates were retrieved from necrotic lesions of potato tubers harvested from different regions of the Canadian provinces New-Brunswick, Nova Scotia and Prince-Edward-Island. Most isolates were closely related to known pathogenic S. scabies strains on the basis of partial 16S ribosomal (r) RNA and rpoB gene sequence analyses. Two isolates were identified as pathogenic species of Streptomyces acidiscabies. To our knowledge, this species has never been previously isolated in these areas. Genome fingerprinting studies using repetitive elements (rep) polymerase chain reactions (PCR) revealed 10 distinct genetic groups in eastern Canada. The geographical distribution of the genetic groups was region-dependant. Pathogenicity- and virulence-related genes (txtA, txtC, and tomA) were PCR-amplified from each isolate, and nucleotide sequence analysis of partial gene fragments revealed slight polymorphisms in both txtA and txtC genes. No genetic variation was noted in the partial tomA gene sequences.
St-Onge
Renée
R
Department of Biology, Université de Moncton, 165 Massey Avenue, Moncton, NB, Canada E1A 3E9.
Goyer
Claudia
C
Coffin
Robert
R
Filion
Martin
M
D001426:Bacterial Proteins / Q000235:genetics; D002170:Canada; D016172:DNA Fingerprinting; D005798:Genes, Bacterial; D014644:Genetic Variation; D044404:Genomic Islands / Q000235:genetics; D008543:Melanins / Q000235:genetics; D008969:Molecular Sequence Data; D010641:Phenotype; D010935:Plant Diseases / Q000382:microbiology*; D035281:Plant Tubers / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011198:Solanum tuberosum / Q000382:microbiology*; D013302:Streptomyces / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity
D001426:Bacterial Proteins; D008543:Melanins; D012336:RNA, Ribosomal, 16S