Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase: a chemistry-biology interdisciplinary study of the protein's structure-function-reaction mechanism relationships.
The oxidosqualene cyclases (EC 5.4.99-) constitute a family of enzymes that catalyze diverse cyclization/rearrangement reactions of (3S)-2,3-oxidosqualene into a distinct array of sterols and triterpenes. The relationship between the cyclization mechanism and the enzymatic structure is extremely complex and compelling. This review covers the historical achievements of biomimetic studies and current progress in structural biology, molecular genetics, and bioinformatics studies to elucidate the mechanistic and structure-function relationships of the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase-catalyzed cyclization/rearrangement reaction.
Wu
Tung-Kung
TK
Department of Biological Science and Technology, National Chiao Tung University, 75, Po-Ai Street, Hsin-Chu 300, Taiwan, ROC. tkwmll@mail.nctu.edu.tw
Chang
Cheng-Hsiang
CH
Liu
Yuan-Ting
YT
Wang
Tsai-Ting
TT
D002384:Catalysis; D003500:Cyclization; D019751:Intramolecular Transferases / Q000737:chemistry*; D008968:Molecular Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013185:Squalene / Q000031:analogs & derivatives / Q000737:chemistry; D013237:Stereoisomerism; D013261:Sterols / Q000138:chemical synthesis / Q000737:chemistry; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000138:chemical synthesis / Q000737:chemistry
C002821:2,3-oxidosqualene; D013261:Sterols; D014315:Triterpenes; D013185:Squalene; D019751:Intramolecular Transferases; C021924:lanosterol synthase