title: Hepatitis B virus-X protein recruits histone deacetylase 1 to repress insulin-like growth factor binding protein 3 transcription.
abstract: Hepatitis B virus (HBV), a major causative agent of hepatocelluar carcinoma (HCC), encodes an oncogenic X-protein (HBx) which has been known as a transcriptional transactivator on multiple viral and celluar promoters. In the report, we verified that HBx transcriptionally repress insulin-like growth factor binding protein-3 (IGFBP-3) by promoting HBx/histone deacetylase 1 (HDAC1) complex formation. HBx recruited HDAC1 forms complex with Sp1 in a p53-independent manner) and deacetylates Sp1 which resulted in the diminished binding of Sp1 on targeted DNA during transcriptional repression. Deacetylation of Sp1 by HBx recruited HDAC1 likely to be a part of the mechanism that controls HBx induced IGFBP-3 repression and the modification of chromatin structure.
authors.affiliation: Liver Cell Signal Transduction Laboratory, Metabolic Syndrome Research Center, Korea Research Institute of Bioscience and Biotechnology, Liver Research Division, Lee's Biotechnology Institute of Research and Development, Taejeon 305-606, Republic of Korea.
authors.forename: Jin Kyung
authors.initials: JK
authors.lastname: Shon
authors.forename: Bo Hwa
authors.initials: BH
authors.lastname: Shon
authors.forename: In Young
authors.initials: IY
authors.lastname: Park
authors.forename: Su Ui
authors.initials: SU
authors.lastname: Lee
authors.forename: Liu
authors.initials: L
authors.lastname: Fa
authors.forename: Keun Young
authors.initials: KY
authors.lastname: Chang
authors.forename: Je Hoon
authors.initials: JH
authors.lastname: Shin
authors.forename: Young Ik
authors.initials: YI
authors.lastname: Lee
mesh_terms: D002478:Cells, Cultured; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000378:metabolism*; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000037:antagonists & inhibitors*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D012097:Repressor Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases