Identification of beta-1,4-galactosyltransferase I as a target gene of HBx-induced cell cycle progression of hepatoma cell.
OBJECTIVE
The hepatitis B virus-encoded HBx protein contributes to hepatocarcinogenesis with largely unknown mechanisms. It is widely known that N-linked oligosaccharides on glycoproteins are structurally altered during malignant transformation and these alterations are often associated with malignant transformation of cells. beta-1,4-galactosyltransferase I (GalT I) contributes to the biosynthesis of Galbeta-->4GlcNAc structure in the outer chain moieties of N-glycans.


METHODS
The difference of GalT I expression between normal liver and hepatoma tissues were investigated; the effect of HBx on GalT I expression was investigated; the role of GalT I in hepatoma cell growth and HBx-induced hepatoma cell growth were investigated.


RESULTS
GalT I was highly expressed in hepatocellular carcinoma and transcriptionally up-regulated by HBx, and functioned as a positive growth regulator in hepatoma cells. Furthermore, decreasing the expression of GalT I in hepatoma cells reduced the ability of tumor formation in vivo and inhibited HBx-induced cell cycle progression.


CONCLUSIONS
HBx-induced GalT I expression might contribute to HBx-mediated HCC development and progression.
Wei
Yuanyan
Y
Key Laboratory of Glycoconjuates Research, Ministry of Public Health, Gene Research Center Shanghai Medical College of Fudan University, Shanghai 200032, People's Republic of China.
Liu
Dan
D
Zhou
Fengbiao
F
Ge
Yuqing
Y
Xu
Jiejie
J
Yun
Xiaojing
X
Gu
Jianxin
J
Jiang
Jianhai
J
D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D005260:Female; D005700:Galactosyltransferases / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D006509:Hepatitis B / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008099:Liver / Q000473:pathology / Q000502:physiology; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D014158:Transcription, Genetic / Q000502:physiology
D005700:Galactosyltransferases; C119701:beta-1,4-galactosyltransferase I