title: Modulatory effects of hard clam (Mercenaria mercenaria) tissue extracts on the in vitro growth of its pathogen QPX.
abstract: Quahog parasite unknown (QPX) is a fatal protistan parasite affecting cultured and wild hard clams Mercenaria mercenaria along the northeastern coasts of the USA and maritime Canada. Field investigations and laboratory transmission studies revealed some variations in the susceptibility of different hard clam stocks to QPX infection. In this study, we used in vitro QPX cultures to investigate the effect of plasma and tissue extracts from two different clam stocks on parasite survival and growth. Results demonstrated the presence of factors in clams that significantly modulate QPX growth. Extracts from gills and mantle tissues as well as plasma inhibited in vitro QPX growth, whereas foot and adductor muscle extracts enhanced parasite growth. Investigations of anti-QPX activities in plasma from two clam stocks displaying different susceptibility toward QPX disease in vivo demonstrated higher inhibition of QPX growth by plasma from New York (resistant) clams compared to Florida (susceptible) clams. Some clams appeared to be deficient in inhibitory factors, suggesting that such animals may become more easily infected by the parasite.
authors.affiliation: School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY 11794-5000, USA.
authors.forename: Mickael
authors.initials: M
authors.lastname: Perrigault
authors.forename: Deenie M
authors.initials: DM
authors.lastname: Buggé
authors.forename: Chen Chuan
authors.initials: CC
authors.lastname: Hao
authors.forename: Bassem
authors.initials: B
authors.lastname: Allam
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004198:Disease Susceptibility; D006790:Host-Parasite Interactions; D049891:Mercenaria / Q000737:chemistry / Q000378:metabolism / Q000469:parasitology*; D010271:Parasites / Q000187:drug effects / Q000502:physiology*; D010949:Plasma / Q000469:parasitology; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D014020:Tissue Extracts