title: Bioavailability and kinetics of sulforaphane in humans after consumption of cooked versus raw broccoli.
abstract: The aim of this study was to determine the bioavailability and kinetics of the supposed anticarcinogen sulforaphane, the hydrolysis product of glucoraphanin, from raw and cooked broccoli. Eight men consumed 200 g of crushed broccoli, raw or cooked, with a warm meal in a randomized, free-living, open cross-over trial. Higher amounts of sulforaphane were found in the blood and urine when broccoli was eaten raw (bioavailability of 37%) versus cooked (3.4%, p ) 0.002). Absorption of sulforaphane was delayed when cooked broccoli was consumed (peak plasma time ) 6 h) versus raw broccoli (1.6 h, p ) 0.001). Excretion half-lives were comparable, 2.6 and 2.4 h on average, for raw and cooked broccoli, respectively (p ) 0.5). This study gives complete kinetic data and shows that consumption of raw broccoli results in faster absorption, higher bioavailability, and higher peak plasma amounts of sulforaphane, compared to cooked broccoli.
authors.affiliation: TNO Quality of Life, AJ Zeist, The Netherlands.
authors.forename: Martijn
authors.initials: M
authors.lastname: Vermeulen
authors.forename: Ineke W A A
authors.initials: IW
authors.lastname: Klöpping-Ketelaars
authors.forename: Robin
authors.initials: R
authors.lastname: van den Berg
authors.forename: Wouter H J
authors.initials: WH
authors.lastname: Vaes
mesh_terms: D000293:Adolescent; D000328:Adult; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001682:Biological Availability; D001937:Brassica / Q000737:chemistry*; D018592:Cross-Over Studies; D004032:Diet; D005511:Food Handling / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D017879:Isothiocyanates; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D013454:Sulfoxides; D013861:Thiocyanates / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D013454:Sulfoxides; D013861:Thiocyanates; C016766:sulforaphane