Cell death upon H(2)O(2) induction in the unicellular green alga Micrasterias.
In the present study, we investigated whether the unicellular green alga Micrasterias denticulata is capable of executing programmed cell death (PCD) upon experimental induction, and which morphological, molecular and physiological hallmarks characterise this. This is particularly interesting as unicellular freshwater green algae growing in shallow bog ponds are exposed to extreme environmental conditions, and the capacity to perform PCD may be an important strategy to guarantee survival of the population. The theoretically 'immortal' alga Micrasterias is an ideal object for such investigations as it has served as a cell biological model system for many years and details on its growth properties, physiology and ultrastructure throughout the cell cycle are well known. Treatments with low concentrations of H(2)O(2) are known to induce PCD in other organisms, resulting in severe ultrastructural changes to organelles, as observed in TEM. These include deformation and part disintegration of mitochondria, abnormal dilatation of cisternal rims of dictyosomes, occurrence of multivesicular bodies, an increase in the number of ER compartments, and slight condensation of chromatin. Additionally, a statistically significant increase in caspase-3-like activity was detected, which was abrogated by a caspase-3 inhibitor. Photosynthetic activity measured by fast chlorophyll fluorescence decreased as a consequence of H(2)O(2) exposure, whereas pigment composition, except for a reduction in carotenoids, was the same as in untreated controls. TUNEL positive staining and ladder-like degradation of DNA, both frequently regarded as a hallmark of PCD in higher plants, could only be detected in dead Micrasterias cells.
Darehshouri
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A
Plant Physiology Division, Cell Biology Department, University of Salzburg, Hellbrunnerstrasse 34, Salzburg, Austria.
Affenzeller
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M
Lütz-Meindl
U
U
D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle; D000460:Chlorophyta / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D053938:DNA Fragmentation / Q000187:drug effects; D048028:DNA, Algal / Q000302:isolation & purification; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D046529:Microscopy, Electron, Transmission; D010788:Photosynthesis; D010860:Pigments, Biological / Q000378:metabolism
D048028:DNA, Algal; D010860:Pigments, Biological; D006861:Hydrogen Peroxide; D053148:Caspase 3