title: Metabolite imaging using matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS).
abstract: We describe here the use of a hybrid ionization approach, matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS) in bioimaging. ME-SALDI combines the strengths of traditional matrix-assisted laser desorption/ionization (MALDI) and SALDI and enables successful MS imaging of low-mass species with improved detection sensitivity. Using 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as the MS standard, MS performances of MALDI, SALDI, and ME-SALDI are systematically compared. The analyte desorption and ionization mechanism in ME-SALDI is qualitatively speculated based on the observation of significantly reduced matrix background and improved survival yields of molecular ions. Improvements in detection sensitivity of low-mass species using ME-SALDI over MALDI in imaging are demonstrated with mouse heart and brain tissues.
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Xiao
authors.forename: Coral
authors.initials: C
authors.lastname: Pagan-Miranda
authors.forename: Yu Matthew
authors.initials: YM
authors.lastname: Chiu
authors.forename: Lin
authors.initials: L
authors.lastname: He
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002531:Cerebellum / Q000737:chemistry; D054022:Cerebrum / Q000737:chemistry; D003952:Diagnostic Imaging / Q000379:methods*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005260:Female; D051379:Mice; D009206:Myocardium / Q000737:chemistry*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004134:Dimyristoylphosphatidylcholine