title: The better tag remains unseen.
abstract: One of the most crucial steps in protein structure determination by nuclear magnetic resonance (NMR) spectroscopy is the preparation of highly concentrated and well behaving protein samples. Here we present a system of modular tags which allows for high level expression, sophisticated purification of full-length protein, and solubility enhancement while keeping the amount of additional resonances low. This system consists of two different expression constructs and utilizes the tight binding of human calmodulin (hCaM) to the calmodulin binding peptide (CBP), which has already been used as a purification tag.
authors.affiliation: Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, and Cluster of Excellence Macromolecular Complexes (CEF), University of Frankfurt, Max-von-Laue Strasse 9, 60438 Frankfurt am Main, Germany
authors.forename: Florian G
authors.initials: FG
authors.lastname: Durst
authors.forename: Horng Der
authors.initials: HD
authors.lastname: Ou
authors.forename: Frank
authors.initials: F
authors.lastname: Löhr
authors.forename: Volker
authors.initials: V
authors.lastname: Dötsch
authors.forename: Wesley E
authors.initials: WE
authors.lastname: Straub
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002147:Calmodulin / Q000737:chemistry*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry*; D005982:Glutathione Transferase / Q000737:chemistry; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010446:Peptide Fragments / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase