title: A novel l-amino acid ligase from bacillus licheniformis.
abstract: l-Amino acid alpha-ligase (EC 6.3.2.28) catalyzed formation of alpha-peptide bond in unprotected l-amino acids in an ATP-dependent manner. BL00235 gene in Bacillus licheniformis NBRC12200 coded as a new l-amino acid ligase. BL00235 substrate specificity was strict; only methionine or leucine was acceptable as dipeptide N-terminal residues.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.
authors.forename: Kuniki
authors.initials: K
authors.lastname: Kino
authors.forename: Atsushi
authors.initials: A
authors.lastname: Noguchi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakazawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Yagasaki
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000737:chemistry*
chemical_list: D010453:Peptide Synthases