Behavioural responses and expression of P2X3 receptor in trigeminal ganglion after experimental tooth movement in rats.
OBJECTIVE
To explore the role of P2X(3) receptor in pain induced by experimental tooth movement.


METHODS
Male Sprague-Dawley rats weighing 200-300g were used. P2X(3) receptor distribution in the caudal one-third portion of the trigeminal ganglion (TG) was studied by IHC. Next, the changes of P2X(3) expression were detected by Western blotting 4h, 1d, 2d, 3d, 5d, 7d, 14d after tooth movement. We then developed a behaviour pain model associated with directed mouth wiping. Finally, the effect of TNP-ATP on nociceptive-like behaviour was evaluated.


RESULTS
Our results showed that P2X(3) receptors were expressed mainly in small- and medium-sized cells and experimental tooth movement led to an increase in staining of mandibular P2X(3) receptors. In addition, following experimental tooth movement, the expression of P2X(3) receptor in TG was statistically significantly up-regulated from days 1 to 5, with a peak on day 3. It was also found that the time spent on directed mouth wiping was dramatically increased by experimental tooth movement from days 1 to 7. The rhythm change of P2X(3) receptor expression in TG and the mouth wiping behaviour were in concert with the initial orthodontic pain responses. The directed mouth wiping behaviour was modulated in a force-dependent manner and could be attenuated by peripheral and systemic morphine. Furthermore, peripherally administered TNP-ATP could exert an analgesic effect on this pain model.


CONCLUSIONS
These results suggest that directed mouth wiping behaviour can be a reliable measurement of pain following experimental tooth movement in rats. The P2X(3) receptor is important in the development and maintenance of tooth movement pain and thus may be peripheral targets for analgesics in orthodontic pain control.
Yang
Zhi
Z
State Key Laboratory of Oral Diseases and Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu, China.
Cao
Yang
Y
Wang
Yan
Y
Luo
Wei
W
Hua
Xiaochuan
X
Lu
Yun
Y
Liao
Zhengyu
Z
Lai
Wenli
W
Zhao
Zhihe
Z
D000255:Adenosine Triphosphate / Q000008:administration & dosage / Q000031:analogs & derivatives; D000701:Analgesics, Opioid / Q000008:administration & dosage; D000818:Animals; D005456:Fluorescent Dyes / Q000008:administration & dosage; D015870:Gene Expression; D006120:Grooming / Q000502:physiology*; D008297:Male; D009020:Morphine / Q000008:administration & dosage; D017288:Pain Threshold; D058919:Purinergic P2 Receptor Antagonists; D051381:Rats; D017207:Rats, Sprague-Dawley; D018048:Receptors, Purinergic P2 / Q000032:analysis / Q000378:metabolism*; D058477:Receptors, Purinergic P2X3; D014087:Tooth Movement Techniques / Q000009:adverse effects; D014098:Toothache / Q000188:drug therapy / Q000503:physiopathology; D012668:Trigeminal Ganglion / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology
D000701:Analgesics, Opioid; D005456:Fluorescent Dyes; D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; C045674:2',3'-O-(2,4,6-trinitro-cyclohexadienylidine)adenosine 5'-triphosphate; D009020:Morphine; D000255:Adenosine Triphosphate