Screening and identification of a novel B-cell neutralizing epitope from Helicobacter pylori UreB.
Urease plays a crucial role in the survival and pathogenesis of Helicobacter pylori (H. pylori), and antibody neutralizing the urease activity may be implicated for the protection against H. pylori infection. Previously, a neutralizing monoclonal antibody (MAb) 6E6 against UreB of H. pylori was developed. In this work, we try to identify the B-cell epitope recognized by neutralizing MAb 6E6. Following screening a series of truncated proteins of UreB, an epitope was primarily localized in the aa 200-230 of UreB. Subsequently, we screened the overlapping synthetic peptides covering the aa 200-230 and identified a novel B-cell epitope (U(211-225), IEAGAIGFKIHEDWG) that was recognized by specific MAb 6E6. The newly identified epitope may help understanding of the protective immunity against H. pylori and be implicated for vaccine development.
Li
Hai-Xia
HX
Department of Clinical Microbiology and Clinical Immunology, The Third Military Medical University, Chongqing 400038, People's Republic of China.
Mao
Xu-Hu
XH
Shi
Yun
Y
Ma
Ying
Y
Wu
Ya-Nan
YN
Zhang
Wei-Jun
WJ
Luo
Ping
P
Yu
Shu
S
Zhou
Wei-Ying
WY
Guo
Ying
Y
Wu
Chao
C
Guo
Gang
G
Zou
Quan-Ming
QM
D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D015153:Blotting, Western; D004353:Drug Evaluation, Preclinical; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D016481:Helicobacter Infections / Q000276:immunology; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D008968:Molecular Conformation; D009500:Neutralization Tests; D011487:Protein Conformation; D014510:Urease / Q000037:antagonists & inhibitors / Q000276:immunology*
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines; D000939:Epitopes; D014510:Urease