[Proof of transovarial transmission of Lymantria dispar nucleopolyhedrovirus (fam. Baculoviridae) with the RAPD-PCR method].
Transovarial transmission of Lymantria dispar Nucleopolyhedrovirus is shown with the RAPD-PCR method. Genetic markers of virus DNA found with the primer OPA-08 are recommended for diagnostics of Lymantria dispar nucleopolyhedrosis, in particular at the egg stage. Genetic markers of virus DNA found with the use of OPA-08 primer are helpful for estimation of genetic diversity of virus, level of the virus presence among the eggs, and for a decision about forestry protective measures.
Oberemok
V V
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D000818:Animals; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D014644:Genetic Variation; D007915:Lepidoptera / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000302:isolation & purification*; D010063:Ovum / Q000821:virology*; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D004279:DNA, Viral