A novel medium for the isolation of N-acylhomoserine lactone-degrading bacteria.
A novel chemically defined medium, named KG medium, supplemented with N-3-oxo-hexanoylhomoserine lactone (3-oxo-C6-HSL), an acylhomoserine lactone (AHL) used as signalling molecules in Gram-negative bacterial cell-to-cell communication, as the sole source of carbon and nitrogen, was designed and successfully used for the enrichment and isolation of AHL-degrading bacteria. A 3-oxo-C6-HSL-degrading bacterium, 13sw7, was isolated from sewage after six enrichment transfers in the 3-oxo-C6-HSL-containing KG medium. On the basis of the almost complete 16S ribosomal DNA sequence, isolate 13sw7 was clustered with unculturable beta-proteobacteria. This study indicates that the AHL-containing KG medium is effective in isolating AHL-degrading bacteria, including those previously considered unculturable, from environmental sources. To the best of our knowledge, this is the first documentation of the isolation of an AHL-degrading proteobacterium from sewage.
Chan
Kok-Gan
KG
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. kokgan@um.edu.my
Yin
Wai-Fong
WF
Sam
Choon-Kook
CK
Koh
Chong-Lek
CL
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D001431:Bacteriological Techniques; D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000737:chemistry*; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D053038:Quorum Sensing; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D003470:Culture Media; D012722:Sewage; C088386:homoserine lactone; D015107:4-Butyrolactone