title: A noncytotoxic DsRed variant for whole-cell labeling.
abstract: A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Chicago, Gordon Center W238, Chicago, Illinois 60637, USA.
authors.forename: Rita L
authors.initials: RL
authors.lastname: Strack
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Strongin
authors.forename: Dibyendu
authors.initials: D
authors.lastname: Bhattacharyya
authors.forename: Wen
authors.initials: W
authors.lastname: Tao
authors.forename: Allison
authors.initials: A
authors.lastname: Berman
authors.forename: Hal E
authors.initials: HE
authors.lastname: Broxmeyer
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Keenan
authors.forename: Benjamin S
authors.initials: BS
authors.lastname: Glick
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis* / Q000633:toxicity; D008969:Molecular Sequence Data; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D008164:Luminescent Proteins; C413663:fluorescent protein 583