Association study between adolescent idiopathic scoliosis and the DPP9 gene which is located in the candidate region identified by linkage analysis.
BACKGROUND
It has been recognised that genetic or hereditary factors may contribute to the aetiology of adolescent idiopathic scoliosis (AIS). Recently, two linkage analyses have identified 19p13.3 as the candidate region for AIS. The dipeptidyl peptidase 9 (DPP9) gene is located on chromosome 19p13.3.


OBJECTIVE
To investigate whether DPP9 gene polymorphisms are associated with the occurrence or curve severity of AIS.


METHODS
571 girls with AIS and 236 normal controls were recruited. Using the Chinese data from the HapMap project, a set of tagging single-nucleotide polymorphisms (tagSNPs) were defined for the DPP9 gene. Five SNPs were genotyped by PCR restriction fragment length polymorphism. Statistical analysis of genotype frequencies between cases and controls was performed by the chi2 test. One-way analysis of variance was used to compare mean maximum Cobb angles with different genotypes in case-only analysis.


RESULTS
Genotype frequencies were comparable between cases and controls for all five polymorphisms (p>0.05). The mean maximum Cobb angles of different genotypes were similar to each other for all five polymorphisms.


CONCLUSIONS
The DPP9 gene is not associated with the occurrence or curve severity of AIS. It is neither a disease-predisposition nor a disease-modifying gene of AIS.
Qiu
X S
XS
Department of Orthopaedics, The Affiliated Drum Town Hospital of Nanjing University Medical School, Nanjing, China. cnspine@gmail.com
Tang
N L S
NL
Yeung
H Y
HY
Qiu
Y
Y
Cheng
J C Y
JC
D000293:Adolescent; D002648:Child; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage / Q000235:genetics; D006801:Humans; D011110:Polymorphism, Genetic / Q000235:genetics; D012600:Scoliosis / Q000235:genetics*
C471241:DPP9 protein, human; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases