title: Oral administration of choline does not affect metabolic characteristics of gliomas and normal-appearing white matter, as detected with single-voxel (1)H-MRS at 1.5 T.
abstract: BACKGROUND
  The present study was done for evaluation of the possible influence of the oral administration of choline on metabolic characteristics of gliomas detected with proton magnetic resonance spectroscopy ((1)H-MRS).
  
  
  METHODS
  Thirty patients (22 men and eight women; mean age 38 +/- 15 years) with suspicious intracranial gliomas underwent single-voxel long-echo (TR 2,000 ms, TE 136 ms, 128-256 acquisitions) (1)H-MRS of the tumor, peritumoral brain tissue, and distant normal-appearing white matter before and several hours (median, 3 h; range, 1.2-3.7 h) after ingestion of choline with prescribed dose of 50 mg/kg (median actual dose, 52 mg/kg; range, 48-78 mg/kg). Investigations were done using 1.5 T clinical magnetic resonance imager. The volume of the rectangular (1)H-MRS voxel was either 3.4 or 8 cm(3). At the time of both spectroscopic examinations, similar voxels' positioning and size and technical parameters of (1)H-MRS were used. Surgery was done in 27 patients within 1 to 68 days thereafter. In all cases, more than 80% resection of the neoplasm was attained.
  
  
  RESULTS
  There were 12 low-grade gliomas and 15 high-grade gliomas. MIB-1 index varied from 0% to 51.7% (median, 13.8%). Statistical analysis did not disclose significant differences of any investigated metabolic parameter of the tumor, peritumoral brain tissue and distant normal-appearing white matter between two spectroscopic examinations.
  
  
  CONCLUSIONS
  Single-voxel (1)H-MRS at 1.5 T could not detect significant changes of the metabolic characteristics of gliomas, peritumoral brain tissue, and distant normal-appearing white matter after oral administration of choline.
authors.affiliation: International Research and Educational Institute for Integrated Medical Sciences (IREIIMS), Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan. m_chernov@yahoo.com
authors.forename: Mikhail F
authors.initials: MF
authors.lastname: Chernov
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Muragaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Maruyama
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ono
authors.forename: Masao
authors.initials: M
authors.lastname: Usukura
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iseki
authors.forename: Osami
authors.initials: O
authors.lastname: Kubo
authors.forename: Tomokatsu
authors.initials: T
authors.lastname: Hori
authors.forename: Kintomo
authors.initials: K
authors.lastname: Takakura
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology*; D002794:Choline / Q000008:administration & dosage*; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D009413:Nerve Fibers, Myelinated / Q000187:drug effects / Q000473:pathology*; D011522:Protons
chemical_list: D011522:Protons; D002794:Choline