title: Oral tolerance attenuates airway inflammation and remodeling in a model of chronic pulmonary allergic inflammation.
abstract: We investigated the effects of oral tolerance (OT) in controlling inflammatory response, hyperresponsiveness and airway remodeling in guinea pigs (GP) with chronic allergic inflammation. Animals received seven inhalations of ovalbumin (1-5mg/mL-OVA group) or normal saline (NS group). OT was induced by offering ad libitum ovalbumin 2% in sterile drinking water starting with the 1st ovalbumin inhalation (OT1 group) or after the 4th (OT2 group). The induction of OT in sensitized animals decreased the elastance of respiratory system (Ers) response after both antigen and methacholine challenges, peribronchial edema formation, eosinophilic airway infiltration, eosinophilopoiesis, and airways collagen and elastic fiber content compared to OVA group (P<0.05). The number of mononuclear cells and resistance of respiratory system (Rrs) responses after antigen and methacholine challenges were decreased only in OT2 group compared to OVA group (P<0.05). Concluding, our results show that inducing OT attenuates airway remodeling as well as eosinophilic inflammation and respiratory system mechanics.
authors.affiliation: Department of Medicine, School of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Viviane C
authors.initials: VC
authors.lastname: Ruiz Schütz
authors.forename: Tatiana
authors.initials: T
authors.lastname: Drewiacki
authors.forename: Adriane S
authors.initials: AS
authors.lastname: Nakashima
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Arantes-Costa
authors.forename: Carla M
authors.initials: CM
authors.lastname: Prado
authors.forename: David I
authors.initials: DI
authors.lastname: Kasahara
authors.forename: Edna A
authors.initials: EA
authors.lastname: Leick-Maldonado
authors.forename: Mílton A
authors.initials: MA
authors.lastname: Martins
authors.forename: Iolanda F L C
authors.initials: IF
authors.lastname: Tibério
mesh_terms: D000280:Administration, Inhalation; D000403:Airway Resistance; D000704:Analysis of Variance; D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D004547:Elastic Tissue / Q000378:metabolism; D004804:Eosinophils / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism; D006168:Guinea Pigs; D006967:Hypersensitivity / Q000276:immunology*; D008168:Lung / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D011014:Pneumonia / Q000150:complications / Q000188:drug therapy / Q000276:immunology*; D015656:Respiratory Mechanics / Q000187:drug effects / Q000276:immunology; D013997:Time Factors
chemical_list: D010047:Ovalbumin