Molecular pharmacological basis of the YiSui ShenXu Granule in beta-thalassemia therapy.
OBJECTIVE
To study the molecular pharmacological basis of the YiSui ShenXu Granule, a complex prescription of the Chinese traditional medicine used to treat beta-thalassemia.


METHODS
Real-time quantitative PCR method had been applied to analyze the genes expression: gamma-globin, Ckit, EpoR, Spi, FKLF, GATA1 and GATA2 in K562 cell treated and untreated with this complex prescription and its each single herbal medicine.


RESULTS
The results showed that this complex prescription increased the gamma-globin, EpoR, Spi and FKLF expression and decreased the Ckit, GATA1 and GATA2 expression. And all single herbal medicines of this complex prescription could change some of those gene expressions, but not the same as the complex prescription. Even that, this study results indicated that the YiSui ShenXu Granule has its molecular pharmacological basis in treating beta-thalassemia.
Zhang
Chong
C
Molecular Biology Laboratory of Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
Wu
Zhi-Kui
ZK
D029971:Angelica sinensis; D051017:Apoptosis Regulatory Proteins; D018797:Cell Cycle Proteins / Q000235:genetics; D045744:Cell Line, Tumor; D017931:DNA Primers; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D050982:GATA1 Transcription Factor / Q000235:genetics; D050989:GATA2 Transcription Factor / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells; D008516:Medicine, Chinese Traditional; D016133:Polymerase Chain Reaction; D055656:Prescriptions; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017467:Receptors, Erythropoietin / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D017086:beta-Thalassemia / Q000188:drug therapy*; D005719:gamma-Globulins / Q000235:genetics
D051017:Apoptosis Regulatory Proteins; D018797:Cell Cycle Proteins; D017931:DNA Primers; D004365:Drugs, Chinese Herbal; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D050989:GATA2 Transcription Factor; C494711:GATA2 protein, human; C115117:KLF11 protein, human; D012333:RNA, Messenger; D017467:Receptors, Erythropoietin; D012097:Repressor Proteins; D005719:gamma-Globulins; C527854:yisui shengxue; C044613:angelicae sinensis extract; D019009:Proto-Oncogene Proteins c-kit