title: Human Neutrophil Peptides 1-3--early markers in development of colorectal adenomas and carcinomas.
abstract: Expression of Human Neutrophil Peptides (HNP) 1-3 was recently found to be associated with development of colorectal cancer. Raised defensin-expression in tumours is believed to stem from increased infiltration of neutrophils into tumour environment. To further specify the role of alpha-defensins in tumourigenesis and progression, HNP1-3 were analyzed in colorectal adenomas and carcinomas of 87 patients and quantified in relation to cancer stage and grading. Using the ProteinChip arrays, HNP1-3 were found upregulated in both colorectal adenomas and carcinomas. By combining the array with Laser capture microscopy we were able to confirm that HNP1-3 are expressed by tumour cells but not by neutrophils or other tumour invading cells. These findings suggest that alpha-defensins are more likely to contribute to tumour growth than they are to mount an effective host anti-tumour response. However, the amount of HNP-expression was not found to be related to tumour stage, grading, and serological tumour markers.
authors.affiliation: Department of General, Visceral and Vascular Surgery, Universitätsklinikum Jena, Jena, Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Mothes
authors.forename: Christian
authors.initials: C
authors.lastname: Melle
authors.forename: Günther
authors.initials: G
authors.lastname: Ernst
authors.forename: Roland
authors.initials: R
authors.lastname: Kaufmann
authors.forename: Ferdinand
authors.initials: F
authors.lastname: von Eggeling
authors.forename: Utz
authors.initials: U
authors.lastname: Settmacher
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000556:secondary; D000236:Adenoma / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009504:Neutrophils / Q000378:metabolism; D011379:Prognosis; D046888:Tissue Array Analysis; D023084:alpha-Defensins / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D023084:alpha-Defensins; C406413:human neutrophil peptide 1; C406416:human neutrophil peptide 2; C406427:human neutrophil peptide 3