title: Fast real-time polymerase chain reaction for quantitative detection of Lactobacillus delbrueckii bacteriophages in milk.
abstract: One of the main microbiological problems of the dairy industry is the susceptibility of starter bacteria to virus infections. Lactobacillus delbrueckii, a component of thermophilic starter cultures used in the manufacture of several fermented dairy products, including yogurt, is also sensitive to bacteriophage attacks. To avoid the problems associated with these viruses, quick and sensitive detection methods are necessary. In the present study, a fast real-time quantitative polymerase chain reaction assay for the direct detection and quantification of L. delbrueckii phages in milk was developed. A set of primers and a TaqMan MGB probe was designed, based on the lysin gene sequence of different L. delbrueckii phages. The results show the proposed method to be a rapid (total processing time 30 min), specific and highly sensitive technique for detecting L. delbrueckii phages in milk.
authors.affiliation: Instituto de Productos Lácteos de Asturias (CSIC), Apdo. de Correos 85, 33300 Villaviciosa, Asturias, Spain.
authors.forename: Maria Cruz
authors.initials: MC
authors.lastname: Martín
authors.forename: Beatriz
authors.initials: B
authors.lastname: del Rio
authors.forename: Noelia
authors.initials: N
authors.lastname: Martínez
authors.forename: Alfonso H
authors.initials: AH
authors.lastname: Magadán
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Alvarez
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000302:isolation & purification*; D002417:Cattle; D003257:Consumer Product Safety; D005285:Fermentation; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D048668:Lactobacillus delbrueckii / Q000821:virology*; D008892:Milk / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity