A comparison of gene expression profiles between primary human AML cells and AML cell line.
In acute myeloid leukemia (AML), hematologic malignancies are characterized by recurring chromosomal abnormalities. Chromosome translocation t(9;11)(p22;q23) is one of the most common genetic aberrations and results in the formation of the MLL-AF9 fusion gene that functions as a facilitator of cell growth directly. In order to study this type of AML, the cell lines with cytogenetically diagnosed t(9;11)(p22;q23), such as Mono Mac 6 (MM6), have been widely used. To examine whether there is any difference in gene expression between the primary human t(9;11) AML cells and MM6 cell line, genome-wide transcriptome analysis was performed on MM6 cell line using SAGE and the results were compared to the profile of primary human t(9;11) AML cells. 884 transcripts which were alternatively expressed between MM6 cells and primary human t(9;11) cells were identified through statistical analysis (P < 0.05) and 4-fold expression change. Of these transcripts, 830 (94%) matched to known genes or EST were classified by functional categories (http://david.abcc.ncifcrf.gov/). The majority of alternatively expressed genes in MM6 were involved in biosynthetic and metabolic processes, but HRAS, a protein that is known to be associated with leukemogenesis, was expressed only in MM6 cells and several other genes involved in Erk1/Erk2 MAPK pathway were also over-expressed in MM6. Therefore, since MM6 cell line has a similar expression profile to primary human t(9;11) AML in general and expresses uniquely a strong Erk1/Erk2 MAPK pathway including HRAS, it can be used as a model for HRAS-positive t(9;11) AML.
Lee
Jinseok
J
School of Life Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.
Hwang
Junmo
J
Kim
Hyung-Soo
HS
Kim
Seonggon
S
Kim
Young Hun
YH
Park
So-Young
SY
Kim
Kil Soo
KS
Ryoo
Zae Young
ZY
Chang
Kyu-Tae
KT
Lee
Sanggyu
S
D045744:Cell Line, Tumor; D002880:Chromosomes, Human, Pair 11; D002899:Chromosomes, Human, Pair 9; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D015723:Gene Library; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D014178:Translocation, Genetic