Effects of cyclosporine on the production of the reactive oxygen species in the glial cells.
OBJECTIVE
After organ transplantation, some patients suffer mild neurological symptoms such as tremor to severe complications including seizures and encephalopathy. Among the immunosuppressants, cyclosporine (CsA) can induce neurological side effects. However, the mechanisms of encephalopathy by CsA are not fully understood. We measured the production of reactive oxygen species (ROS) in the glioma cells after CsA treatment.


METHODS
CsA (2.5 mmol/L) added to glioma cells was incubated for 60 minutes at 37 degrees C. ROS production was evaluated by measuring the fluorescent product from the oxidation of an oxidant-sensitive 2',7'-dichlorofluorescin using VICTOR3 multilabel counter.


RESULTS
CsA resulted in ROS production by glioma cells. The ROS production increased with the time of exposure to CsA.


CONCLUSIONS
These findings indicated that CsA may contribute to neurological side effects via ROS production.
Mun
K C
KC
Dongsan Kidney Institute and Chronic Disease Research Center, Keimyung University, Daegu, Korea. mun@dsmc.or.kr
Lee
K T
KT
Choi
H J
HJ
Jin
K B
KB
Han
S Y
SY
Park
S B
SB
Kim
H C
HC
Ha
E Y
EY
Kim
Y H
YH
D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D005910:Glioma / Q000378:metabolism*; D007700:Kinetics; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*
D005609:Free Radicals; D017382:Reactive Oxygen Species; D016572:Cyclosporine