title: The analysis of three markers flanking GJB2 gene suggests a single origin of the most common 35delG mutation in the Moroccan population.
abstract: In Caucasian populations a single mutation, 35delG, accounts for the majority of GJB2 gene mediated hearing loss, with carrier frequencies estimated between 2-4%, possibly resulting from a founder effect rather than from a mutational hot spot. In Moroccan population, the 35delG mutation accounts for 90.8% of all GJB2 mutated alleles in deaf patients with a carrier frequency of 2.65%. The aim of this study was to evaluate whether the 35delG mutation has derived from a single origin in the Moroccan population. We enrolled 30 unrelated deaf patients homozygous for the 35delG mutation and 165 unrelated control individuals negative for this mutation, and genotyped three microsatellite markers flanking the GJB2 region: D13S141, D13S175 and D13S143. Data analysis revealed that the 35delG mutation is associated with particular alleles of these markers, with significant linkage disequilibrium for the 125 and 105 nucleotide long alleles of D13S141 and D13S175, and that a single specific haplotype accounts for 68% of the chromosomes carrying the 35delG mutation. The estimate age of 35delG mutation is 135 generations or approximately 2700 years old. Like in other Mediterranean populations, our results suggest that in the Moroccan population the 35delG mutation has derived from a single origin in a common founder process.
authors.affiliation: Laboratoire de Génétique Humaine, Département des Recherches Scientifiques, Institut Pasteur, Morocco. 1, Place Louis Pasteur, Casablanca 20100, Morocco. omar.abidi@pasteur.ma
authors.forename: Omar
authors.initials: O
authors.lastname: Abidi
authors.forename: Redouane
authors.initials: R
authors.lastname: Boulouiz
authors.forename: Halima
authors.initials: H
authors.lastname: Nahili
authors.forename: Laila
authors.initials: L
authors.lastname: Imken
authors.forename: Hassan
authors.initials: H
authors.lastname: Rouba
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Chafik
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Barakat
mesh_terms: D000072259:Connexin 26; D017630:Connexins / Q000235:genetics*; D004252:DNA Mutational Analysis; D018703:Founder Effect; D005819:Genetic Markers; D006312:Hearing Loss, Bilateral / Q000453:epidemiology / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D015994:Incidence; D018598:Minisatellite Repeats / Q000235:genetics; D009018:Morocco / Q000453:epidemiology; D020161:Physical Chromosome Mapping; D017384:Sequence Deletion; D044465:White People / Q000235:genetics
chemical_list: D017630:Connexins; C000607042:GJB2 protein, human; D005819:Genetic Markers; D000072259:Connexin 26