title: Genotypic antiretroviral resistance testing for human immunodeficiency virus type 1 integrase inhibitors by use of the TruGene sequencing system.
abstract: A sequencing assay for detection of mutations conferring resistance to human immunodeficiency virus type 1 (HIV-1) integrase inhibitors raltegravir and elvitegravir was developed using the automated TruGene sequencing system. The assay returned clear sequencing results for samples with >or=500 RNA copies/ml for mutation detection and HIV-1 subtyping across a spectrum of HIV-1 subtypes.
authors.affiliation: Institute of Laboratory Medicine, General Hospital Linz, Linz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Paar
authors.forename: Christina
authors.initials: C
authors.lastname: Palmetshofer
authors.forename: Karin
authors.initials: K
authors.lastname: Flieger
authors.forename: Maria
authors.initials: M
authors.lastname: Geit
authors.forename: Rolf
authors.initials: R
authors.lastname: Kaiser
authors.forename: Herbert
authors.initials: H
authors.lastname: Stekel
authors.forename: Jörg
authors.initials: J
authors.lastname: Berg
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D005838:Genotype; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D019429:Integrase Inhibitors / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D020125:Mutation, Missense; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D019429:Integrase Inhibitors; D012367:RNA, Viral