Selective separation and co-culture of cells by photo-induced enhancement of cell adhesion (PIECA).
Taking advantage of the phenomenon that animal cells adhering to a culture substrate are temporarily immobilized by light irradiation, we established a technique to manipulate the cells adhering to a culture substrate under microscopic observation. Using this technique, we demonstrated a separation of cells adhering to a culture substrate and fabrication of an elaborately patterned co-culture system.
Edahiro
Jun-Ichi
J
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Sumaru
Kimio
K
Ooshima
Yuki
Y
Kanamori
Toshiyuki
T
D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D018920:Coculture Techniques; D006224:Cricetinae; D003412:Cricetulus; D008027:Light