Anti-flagellin antibody responses elicited in mice orally immunized with attenuated Salmonella enterica serovar Typhimurium vaccine strains.
In the present study we investigated the flagellin-specific serum (IgG) and fecal (IgA) antibody responses elicited in BALB/c mice immunized with isogenic mutant derivatives of the attenuated Salmonella enterica serovar Typhimurium (S. Typhimurium) SL3261 strain expressing phase 1 (FliCi), phase 2 (FljB), or no endogenous flagellin. The data reported here indicate that mice orally immunized with recombinant S. Typhimurium strains do not mount significant systemic or secreted antibody responses to FliCi, FljB or heterologous B-cell epitopes genetically fused to FliCi. These findings are particularly relevant for those interested in the use of flagellins as molecular carriers of heterologous antigens vectored by attenuated S. Typhimurium strains.
Massis
L M
LM
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brasil. lmmassis@usp.br
Braga
C J M
CJ
Sbrogio-Almeida
M E
ME
Lauand
C
C
Newton
S M C
SM
Klebba
P E
PE
Ferreira
L C S
LC
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines / Q000276:immunology; D005408:Flagellin / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated; D005408:Flagellin