Combing genomic DNA for structural and functional studies.
Molecular combing is a process whereby single DNA molecules bind by their extremities to a silanised surface and are then uniformly stretched and aligned by a receding air/water interface (1). This method, with a high resolution ranging from a few kilobases to megabases, has many applications in the field of molecular cytogenetics, allowing structural and functional analysis at the genome level. Here we describe protocols for preparing DNA for combing and for the use of fluorescent hybridisation (FH) applied to combed DNA to conduct physical mapping or genomic structural analysis. We also present the methodology for visualising and studying DNA replication using combed DNA.
Schurra
Catherine
C
Unité de Stabilité des Génomes, Institut Pasteur, Paris, France.
Bensimon
Aaron
A
D000818:Animals; D003582:Cytogenetics / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D012821:Silanes / Q000737:chemistry
D012821:Silanes; D004247:DNA