Determination of urinary triclosan by stir bar sorptive extraction and thermal desorption-gas chromatography-mass spectrometry.
We have developed an analytical method for the determination of urinary 5-chloro-2-(2,4-dichlorophenoxy)phenol (triclosan), which utilizes stir bar sorptive extraction (SBSE) and thermal desorption (TD)-gas chromatography-mass spectrometry (GC-MS). Human urine sample is de-conjugated by treatment with beta-glucuronidase and sulfatase. A stir bar coated with polydimethylsiloxane (PDMS) is added to the urine sample in a vial and the sample is stirred for 60 min at room temperature (25 degrees C). Then, the PDMS stir bar is subjected to TD-GC-MS. The detection limit of triclosan is 0.05 ng mL(-1). The method shows linearity over the calibration range (0.1-10 ng mL(-1)) and the correlation coefficient (r) is higher than 0.993 for triclosan standard solution. The average recoveries of triclosan in human urine sample are 102.8-113.1% (RSD: 2.4-6.7%). This simple, sensitive, and selective analytical method may be used in the determination of trace amounts of triclosan in human urine samples.
Kawaguchi
Migaku
M
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Ito
Rie
R
Honda
Hidehiro
H
Endo
Naoyuki
N
Okanouchi
Noriya
N
Saito
Koichi
K
Seto
Yasuo
Y
Nakazawa
Hiroyuki
H
D000891:Anti-Infective Agents, Local / Q000652:urine*; D004129:Dimethylpolysiloxanes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013429:Sulfatases / Q000378:metabolism; D014260:Triclosan / Q000378:metabolism / Q000652:urine*
D000891:Anti-Infective Agents, Local; D004129:Dimethylpolysiloxanes; D014260:Triclosan; C013830:baysilon; D013429:Sulfatases; D005966:Glucuronidase