title: Evaluating new candidate SNPs as low penetrance risk factors in sporadic breast cancer: a two-stage Spanish case-control study.
abstract: OBJECTIVE
  A polygenic model has been proposed in order to explain the genetic susceptibility to sporadic breast cancer. According to this model, common population variants would be responsible for low to modest effects on the risk of developing the disease. We have carried out a high-throughput SNP genotyping project in order to shed some light on the complex genetic aetiology of non-familial breast cancer.
  
  
  METHODS
  Ninety-one genes have been selected because of their implications in several candidate cell pathways for breast cancer. A total of 640 SNPs in these genes were genotyped in a series of 450 consecutive cases and 448 controls from mainland Spain. Promising SNPs were then studied in an independent series of 294 cases and 299 controls from the Canary Islands.
  
  
  RESULTS
  In the first case-control series we identified 25 SNPs with P-values below 0.05 (under a 1 df Chi-square test), five of them with P-values below 0.01 (best=0.0008). In the stage 2 Canary Islands series, odd ratios (OR) for two SNPs in HUS1 were in a consistent direction.
  
  
  CONCLUSIONS
  SNPs located at the gene HUS1 are good candidates for further investigation in independent association studies and functional assays.
authors.affiliation: Fundación Pública Galega de Medicina Xenómica SERGAS, CIBERER, Santiago de Compostela, Galicia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Vega
authors.forename: Antonio
authors.initials: A
authors.lastname: Salas
authors.forename: Roger L
authors.initials: RL
authors.lastname: Milne
authors.forename: Begoña
authors.initials: B
authors.lastname: Carracedo
authors.forename: Gloria
authors.initials: G
authors.lastname: Ribas
authors.forename: Alvaro
authors.initials: A
authors.lastname: Ruibal
authors.forename: Antonio Cabrera
authors.initials: AC
authors.lastname: de León
authors.forename: Ana
authors.initials: A
authors.lastname: González-Hernández
authors.forename: Javier
authors.initials: J
authors.lastname: Benítez
authors.forename: Angel
authors.initials: A
authors.lastname: Carracedo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001943:Breast Neoplasms / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008875:Middle Aged; D019683:Penetrance; D020641:Polymorphism, Single Nucleotide
chemical_list: D018797:Cell Cycle Proteins; C549605:HUS1 protein, human; C462949:HUS1B protein, human