Simple and rapid colorimetric detection of cofactors of aptazymes using noncrosslinking gold nanoparticle aggregation.
We developed a method for simply and rapidly detecting cofactors of aptazymes with high sensitivity using unique noncrosslinking gold nanoparticle aggregation. Applying this method to a theophylline-dependent aptazyme, 100 microM, 10 microM, and 1 microM theophylline were detected easily by the naked eye within 10 min, 20 min, and 65 min, respectively. This method is also applicable to other cleavase-aptazymes without altering the probe-DNA sequence on the gold nanoparticle.
Ogawa
Atsushi
A
Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. a-ogawa@riken.jp
Maeda
Mizuo
M
D015374:Biosensing Techniques / Q000379:methods; D002110:Caffeine / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003124:Colorimetry / Q000379:methods*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004247:DNA / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D015195:Drug Design; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008968:Molecular Conformation; D036103:Nanotechnology / Q000379:methods; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity; D013806:Theophylline / Q000737:chemistry
D003432:Cross-Linking Reagents; D002110:Caffeine; D006046:Gold; D004247:DNA; D013806:Theophylline