title: Role of shear stress on composition, diversity and dynamics of biofilm bacterial communities.
abstract: This article evaluates the effect of shear stress on the composition of biofilm bacterial communities. For the first time, a Conical Couette-Taylor Reactor (CCTR) was used to develop biofilms at varying shear stresses (from 0.055 to 0.27 Pa) and provided a useful model for studying the effect of hydrodynamics on biofilms. The composition, diversity and dynamics of biofilm bacterial communities were analysed using the PCR-SSCP fingerprint method. Results clearly demonstrate a link between shear stress and composition of the microbial communities. High shear stresses decrease biofilm diversity and the analysis of biofilm community dynamics suggests that shear stress would slow down biofilm maturation and tend to maintain a young biofilm.
authors.affiliation: INRA, UR050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Rochex
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Godon
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bernet
authors.forename: Renaud
authors.initials: R
authors.lastname: Escudié
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification; D004867:Equipment Design; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D013314:Stress, Mechanical
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial