title: Immune response and gene polymorphism profiles in Crohn's disease and ulcerative colitis.
abstract: BACKGROUND
  Polymorphisms in genes linked to the innate and adaptive immune response may be involved in inflammatory bowel disease pathogenesis. Our aim was to investigate associations among IL1B-511, IL1B-31, IL1RN, TNFA-307, TLR-2, TLR-4, IL2-330, NOD2 G908R, NOD2 L1007fsinsC polymorphisms and both Crohn's disease (CD) and ulcerative colitis (UC) in a Brazilian population.
  
  
  METHODS
  We studied 43 patients with CD, 42 with UC, and 541 blood donors. Polymorphisms were evaluated by PCR, PCR-CTPP, or PCR-RFLP. Data were analyzed in multivariate models adjusting for confounding factors.
  
  
  RESULTS
  IL1RN VNTR (P = 0.00, odds ratio [OR] = 2.43, 95% confidence interval [CI] = 1.50-3.90), as well as TNFA-307 polymorphic allele (P = 0.05, OR = 1.70, 95% CI = 1.00-2.94) were associated with UC. Both NOD2 mutations (G908R, P = 0.02, OR = 6.83, 95% CI = 1.62-25.45, and L1007fsinsC, P = 0.00, OR = 20.00, 95% CI = 3.21-124.69) were associated with CD.
  
  
  CONCLUSIONS
  Our analyses showed positive associations between proinflammatory polymorphisms at IL1RN and TNFA-307 loci and UC, as well as polymorphisms in the NOD2 gene and CD. These results highlight the importance of different genetic profiles associated with CD and UC.
authors.affiliation: Laboratory of Research in Bacteriology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. dqueiroz@medicina.ufmg.br
authors.forename: Dulciene Maria Magalhães
authors.initials: DM
authors.lastname: Queiroz
authors.forename: Adriana Gonçalves
authors.initials: AG
authors.lastname: Oliveira
authors.forename: Ivan Euclides Borges
authors.initials: IE
authors.lastname: Saraiva
authors.forename: Gifone Aguiar
authors.initials: GA
authors.lastname: Rocha
authors.forename: Andreia Maria Camargos
authors.initials: AM
authors.lastname: Rocha
authors.forename: Maria
authors.initials: M
authors.lastname: das Graças Pimenta Sanna
authors.forename: Juliana Becattini
authors.initials: JB
authors.lastname: Guerra
authors.forename: Renato
authors.initials: R
authors.lastname: Dani
authors.forename: Maria de Lourdes Abreu
authors.initials: Mde L
authors.lastname: Ferrari
authors.forename: Lúcia Porto Fonseca
authors.initials: LP
authors.lastname: Castro
mesh_terms: D000328:Adult; D000483:Alleles; D001938:Brazil / Q000453:epidemiology; D003093:Colitis, Ulcerative / Q000453:epidemiology / Q000235:genetics / Q000276:immunology*; D003424:Crohn Disease / Q000453:epidemiology / Q000235:genetics / Q000276:immunology*; D004247:DNA / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics / Q000378:metabolism; D015810:Linkage Disequilibrium; D008297:Male; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015995:Prevalence; D012189:Retrospective Studies
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein; D004247:DNA