title: Gene expression profiling of peripheral T-cell lymphoma including gammadelta T-cell lymphoma.
abstract: The gene expression profile of peripheral gammadelta T-cell lymphoma (gammadeltaTCL) has not been investigated. Using oligonucleotide microarrays, we analyzed total RNA from 7 patients with gammadeltaTCL (4 hepatosplenic, 1 cutaneous, 1 intestinal, and 1 thyroidal) and 27 patients with alphabetaTCL (11 peripheral TCL-unspecified, 15 angioimmunoblastic TCL, and 1 hepatosplenic). Unsupervised microarray analyses classified all hepatosplenic gammadeltaTCLs into a single cluster, whereas other gammadeltaTCLs were scattered within the alphabetaTCL distribution. We identified a T-cell receptor signature gene set, which accurately classified gammadeltaTCL and alphabetaTCL. A classifier based on gene expression under supervised analysis correctly identified gammadeltaTCL. One case of hepatosplenic alphabetaTCL was placed in the gammadeltaTCL grouping. gammadeltaTCL signature genes included genes encoding killer cell immunoglobulin-like receptors and killer cell lectin-like receptors. Our results indicate that hepatosplenic gammadeltaTCL is a distinct form of peripheral TCL and suggest that nonhepatosplenic gammadeltaTCLs are heterogeneous in gene expression.
authors.affiliation: Department of Hematology, Mie University Graduate School of Medicine, Tsu, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Motoko
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Imai
authors.forename: Tohru
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tamaru
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nishii
authors.forename: Masao
authors.initials: M
authors.lastname: Yuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Katayama
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D016399:Lymphoma, T-Cell / Q000378:metabolism* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016692:Receptors, Antigen, T-Cell, gamma-delta
chemical_list: D009363:Neoplasm Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta