Rwdd1, a thymus aging related molecule, is a new member of the intrinsically unstructured protein family.
We had previously identified a novel protein termed Rwdd1 whose expression in thymus is decreased in aged or oxidatively stressed mice. In the present study, we found that Rwdd1 expressed in both prokaryotic and eukaryotic cells showed a slower migration rate on SDS-PAGE gel. In addition, Rwdd1 was more sensitive to proteinase proteolysis. Furthermore, being a highly acidic protein which contains an RWD domain, Rwdd1 shared a high level of sequence similarity with Gir2, a member of the intrinsically unstructured protein (IUP). These findings suggest that Rwdd1 is a novel member of the IUP family.
Kang
Ning
N
Department of Immunology, School of Basic Medicine, Peking Union Medical College, and Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China.
Chen
Dai
D
Wang
Li
L
Duan
Lian
L
Liu
Shirong
S
Tang
Long
L
Liu
Qingfeng
Q
Cui
Lianxian
L
He
Wei
W
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D013950:Thymus Gland / Q000502:physiology*
D011506:Proteins; C532639:Rwdd1 protein, mouse