Succession of bacterial and fungal communities during natural coffee (Coffea arabica) fermentation.
Bacteria, yeasts and filamentous fungi were isolated during natural coffee processing. Bacteria were isolated in greater numbers at the beginning of the fermentation, when the moisture of the coffee beans was around 68%. Gram-positive bacteria represented 85.5% of all bacteria isolated, and Bacillus was the predominant genus (51%). Gram-negative species of the genera Serratia, Enterobacter and Acinetobacter were also found. Approximately 22% of 940 randomly chosen isolates of microorganisms were yeasts. Debaryomyces (27%), Pichia (18.9%) and Candida (8.0%) were the most commonly found genera, and these three genera tended to appear more often as the fruit was fermented and dried. Aspergillus was the most abundant genus besides Penicillium, Fusarium and Cladosporium, with 42.6% of the total fungi isolates. The genera and species identified included members known to have pectinase and cellulase activities. Of the 10 organic acids analyzed and quantified in coffee beans, acetic and lactic acids may have been generated by microbial activity. Butyric acid was not detected in any sample.
Silva
Cristina Ferreira
CF
Departamento de Biologia, Universidade Federal de Lavras, UFLA, 37.200-000 Lavras, MG, Brazil.
Batista
Luis Roberto
LR
Abreu
Lucas Magalhães
LM
Dias
Eustáquio Souza
ES
Schwan
Rosane Freitas
RF
D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D001938:Brazil; D040503:Coffea / Q000382:microbiology*; D015169:Colony Count, Microbial; D005285:Fermentation; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D005658:Fungi / Q000145:classification / Q000302:isolation & purification*; D010802:Phylogeny; D013997:Time Factors; D015003:Yeasts / Q000145:classification / Q000302:isolation & purification*