Characterization of Bacillus stearothermophilus infA and of its product IF1.
Bacillus stearothermophilus infA encoding translation initiation factor IF1 was cloned and expressed in Escherichia coli and its transcript and protein product characterized. Although the functional properties of B. stearothermophilus and E. coli IF1, compared in several translational tests in the presence of both homologous and heterologous components, are not entirely identical, the two proteins are interchangeable in an in vitro translational system programmed with a natural mRNA. The availability of purified B. stearothermophilus IF1 now allows us to analyze the translation initiation pathway using efficient in vitro tests based entirely on purified components derived from this thermophilic Gram-positive bacterium.
Kapralou
Stavroula
S
Laboratory of Genetics, Department of Biology MCA, University of Camerino, Camerino (MC), Italy.
Fabbretti
Attilio
A
Garulli
Chiara
C
Gualerzi
Claudio O
CO
Pon
Cynthia L
CL
Spurio
Roberto
R
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015977:Eukaryotic Initiation Factor-1 / Q000235:genetics* / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D039861:Prokaryotic Initiation Factor-1 / Q000235:genetics* / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D015977:Eukaryotic Initiation Factor-1; D039861:Prokaryotic Initiation Factor-1; D012329:RNA, Bacterial; D012333:RNA, Messenger