title: Cloning and developmental expression of the soxB2 genes, sox14 and sox21, during Xenopus laevis embryogenesis.
abstract: The Sox family of transcription factors is thought to regulate gene expression in a wide variety of developmental processes. Here we describe the cloning of the X. laevis orthologs of the SoxB2 family of transcription factors, sox14 and sox21. In situ hybridization revealed that sox14 expression is restricted to the hypothalamus, dorsal thalamus, the optic tectum, a region of the somatic motornucleus in the midbrain and hindbrain, the vestibular nuclei in the hindbrain and a discrete ventral domain in the developing spinal cord. In contrast to the limited expression domain of sox14, sox21 is found throughout the developing central nervous system, including the olfactory placodes, with strongest expression at the boundary between the midbrain and hindbrain.
authors.affiliation: Georgetown University, Department of Biology, Washington, DC 20057, USA.
authors.forename: Doreen D
authors.initials: DD
authors.lastname: Cunningham
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Meng
authors.forename: Bernd
authors.initials: B
authors.lastname: Fritzsch
authors.forename: Elena Silva
authors.initials: ES
authors.lastname: Casey
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D006609:High Mobility Group Proteins / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008636:Mesencephalon / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D012249:Rhombencephalon / Q000196:embryology / Q000378:metabolism; D055747:SOX Transcription Factors / Q000235:genetics* / Q000378:metabolism; D055749:SOXB2 Transcription Factors / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000196:embryology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014981:Xenopus / Q000196:embryology* / Q000235:genetics*; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D006609:High Mobility Group Proteins; D012333:RNA, Messenger; D055747:SOX Transcription Factors; D055749:SOXB2 Transcription Factors; C534386:Sox14 protein, Xenopus; C534387:Sox21 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins