[Effect of lipoxins on proliferation and secretion of peritoneal macrophages from patients with preeclampsia in vitro].
OBJECTIVE
To study the effect of lipoxins on the proliferation and secretion of peritoneal macrophages from patients with preeclampsia in vitro.


METHODS
Peritoneal macrophages were obtained from 24 patients with preeclampsia (preeclampsia group) and 24 normal pregnant women (normal pregnant group) who were treated in the First Affiliated Hospital of Wenzhou Medical College from March to July 2007. Enzyme linked immunosorbent assay (ELISA) was used to detect the concentration of tumor necrosis factor-alpha (TNF-alpha) in the supernatant of macrophages which were pulsed with lipoxins at different concentrations (0, 10, 100 nmol/L) in both groups after 48 hours. Methyl thiazolyl tetrazolium (MMTT) assay was used to detect the inhibition rate of cell proliferation of macrophages which were pulsed with lipoxins at different concentrations (0, 10, 100 nmol/L) in both groups after 24 hours.


RESULTS
(1) The concentration of TNF-alpha: the levels of TNF-alpha were (1867.5 +/- 47.3), (1836.9 +/- 4.5) and (1800.5 +/- 2.7) ng/L after treatment with different concentrations of lipoxins (0, 10, 100 nmol/L) in preeclampsia group vs normal pregnant group [(791.3 +/- 62.2), (789.4 +/- 2.3), (781.5 +/- 1.9) ng/L]. The levels of TNF-alpha in preeclampsia group were significantly higher than that in normal pregnant group (P < 0.05). Lipoxins significantly inhibited the concentration of TNF-alpha in a dose-dependent manner in preeclampsia group (P < 0.05), while it had no significant effect in normal pregnant group (P > 0.05). (2) Cell proliferation inhibition: Incubation with lipoxins produced a dose-dependent (0, 10, 100 nmol/L) inhibitory effect on proliferation in preeclampsia group, [ 14. +/-6. )% , (32. +/-3.6)%, ( 6. ++/-3. )% vs normal pregnant group [(16.8 +/- 6.9)%, (16.7 +/- 5.4)%, (15.9 +/- 2.1 )%]. The rate of cell proliferation in preeclampsia group was significantly higher than that in normal pregnant group. Lipoxins significantly inhibited this growth (P < 0.05) , while it had no significant effect in normal pregnant group (P > 0.05).


CONCLUSIONS
Lipoxins can inhibit the proliferation of macrophage and secretion of TNF-alpha in preeclampsia in a dose-dependent manner. Lipoxins may be potentially useful in prevention and treatment of preeclampsia.
Ni
Juan
J
Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, China.
Huang
Yan-Jun
YJ
Wu
Min
M
Liu
Xiao-Li
XL
Zhou
Jie
J
Xiong
Chun-Qqiu
CQ
Dong
Ruo-Lin
RL
Chen
Yun-Qin
YQ
Huang
Yin-Ping
YP
D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D044045:Lipoxins / Q000008:administration & dosage / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D011247:Pregnancy; D012189:Retrospective Studies; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D044045:Lipoxins; D014409:Tumor Necrosis Factor-alpha