Studying isoform-specific inositol 1,4,5-trisphosphate receptor function and regulation.
Inositol 1,4,5-trisphosphate receptors (InsP3R) are a family of ubiquitously expressed intracellular Ca2+ channels. Isoform-specific properties of the three family members may play a prominent role in defining the rich diversity of the spatial and temporal characteristics of intracellular Ca2+ signals. Studying the properties of the particular family members is complicated because individual receptor isoforms are typically never expressed in isolation. In this article, we discuss strategies for studying Ca2+ release through individual InsP3R family members with particular reference to methods applicable following expression of recombinant InsP3R and mutant constructs in the DT40-3KO cell line, an unambiguously null InsP3R expression system.
Betzenhauser
Matthew J
MJ
Department of Pharmacology and Physiology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA.
Wagner
Larry E
LE
Won
Jong Hak
JH
Yule
David I
DI
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D045744:Cell Line, Tumor; D002645:Chickens; D016257:Fura-2 / Q000031:analogs & derivatives / Q000378:metabolism; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000235:genetics / Q000502:physiology*; D010539:Permeability; D010782:Photolysis; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats
D053496:Inositol 1,4,5-Trisphosphate Receptors; D020033:Protein Isoforms; C058776:2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid; D002118:Calcium; D016257:Fura-2