Stress-induced changes in the expression of monocytic beta2-integrins: the impact of arousal of negative affect and adrenergic responses to the Anger Recall Interview.
Adhesion of circulating monocytes to the vascular endothelium is one of the earliest steps in the development of atherosclerosis. This leukocyte-to-endothelium interaction is mediated in part by beta2-integrins, a group of cell adhesion molecules that bind to endothelial ligands. Given the significance of this interaction to atherogenesis, we examined the effects of stress, operationalized as the arousal of negative affect (NA) and cardiovascular and catecholamine responses to the Anger Recall Interview (ARI), on the expression of LFA-1 (CD11a), Mac-1 (CD11b) and p150/95 (CD11c) on circulating monocytes (CD14+). Subjects were 173 healthy, nonsmoking men and women (60% men, 40% minorities, aged 18-49 year). Arousal of NA, cardiovascular responses (heart rate [HR], systolic blood pressure [SBP], diastolic blood pressure [DBP]), circulating catecholamines (epinephrine [Epi], norepinephrine [Ne]) and beta2-integrin (CD11/CD18) expression were determined prior to and following the ARI. The principal findings were that the ARI, on average, induced a decrease in monocyte expression of beta2-integrins. However, after adjusting for age, sex, body mass index, exercise status, and baseline level of beta2-integrin expression, those individuals who showed the largest increases in NA, Ne and DBP during the ARI showed an increase in monocyte beta2-integrin expression. Thus, heightened psychological and physiological stress responses induced phenotypic changes in monocytic expression of beta2-integrins that are consistent with the role of monocytes/macrophages in vascular inflammation and increased risk of atherosclerotic cardiovascular disease.
Greeson
Jeffrey M
JM
Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, NC, USA.
Lewis
James G
JG
Achanzar
Karen
K
Zimmerman
Eugene
E
Young
Kenneth H
KH
Suarez
Edward C
EC
D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D000786:Anger / Q000502:physiology*; D001794:Blood Pressure; D018845:CD11 Antigens / Q000378:metabolism; D018821:CD18 Antigens / Q000378:metabolism*; D004837:Epinephrine / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006339:Heart Rate; D006801:Humans; D007406:Interview, Psychological / Q000379:methods; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008297:Male; D011939:Mental Recall / Q000502:physiology*; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D009638:Norepinephrine / Q000097:blood; D011863:Radioimmunoassay; D013315:Stress, Psychological / Q000209:etiology / Q000276:immunology*; D055815:Young Adult
D018845:CD11 Antigens; D018821:CD18 Antigens; D018950:Lipopolysaccharide Receptors; D009638:Norepinephrine; D004837:Epinephrine