[Clinical proteomics and new biomarkers in biological fluids].
Protein analysis in biological fluids has been used for many years for diagnosis and monitoring of diseases. First it was quantification of total protein, afterwards the electrophoretic separation of proteins and later the quantification of specific proteins using immunoassays. These proteins are used as biological markers (biomarkers) of disease. Since a few years, proteomics allows the simultaneous analysis of hundreds of proteins, as well as the analysis of their structural modifications. In this review the current situation of clinical proteomics for the discovery of new biomarkers in biological fluids is presented.
González-Buitrago
José Manuel
JM
Unidad de Investigación, Hospital Universitario de Salamanca, Salamanca, España. buitrago@usal.es
Ferreira
Laura
L
Muñiz
María Carmen
MC
D000653:Amniotic Fluid / Q000378:metabolism; D001323:Autoantibodies / Q000032:analysis; D015415:Biomarkers / Q000378:metabolism; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis*; D001826:Body Fluids / Q000378:metabolism*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000175:diagnosis / Q000378:metabolism / Q000652:urine; D008297:Male; D013058:Mass Spectrometry; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis / Q000378:metabolism; D011507:Proteinuria / Q000175:diagnosis*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013582:Synovial Fluid / Q000378:metabolism
D001323:Autoantibodies; D015415:Biomarkers; D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins; D020543:Proteome