Association of single nucleotide polymorphisms in the IL-18 gene with production of IL-18 protein by mononuclear cells from healthy donors.
IL-18 has proinflammatory effects and participates in both innate and adaptive cellular and humoral immunity. A number of SNPs that influence IL-18 production are found in the gene promoter region. We investigated the association of SNPs in the IL-18 promoter at -607 and -137 with the level of IL-18 protein production by PBMC from healthy donors from Southwestern Siberia. The genetic distribution of these SNPs in the promoter site was established by PCR. IL-18 protein production was determined by ELISA. Our results showed that PBMC from donors carrying allele 137C have lower levels of both spontaneous and LPS-stimulated IL-18 production. In contrast, PBMC from donors carrying allele 607A showed significant increases in spontaneous and stimulated IL-18 production compared to wild type. Our study suggests that the SNPs -607 and -137 in the promoter region of the IL-18 gene influence the level of IL-18 protein production by PBMC from healthy donors in Southwestern Siberia.
Khripko
Olga Pavlovna
OP
Laboratory of Molecular Immunology, Institute of Clinical Immunology , Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630099, Russia.
Sennikova
Nadegda Sergeevna
NS
Lopatnikova
Julia Anatolievna
JA
Khripko
Jurii Ivanovich
JI
Filipenko
Maxim Leonidovich
ML
Khrapov
Evgenii Alexandrovich
EA
Gelfgat
Evgenii L'vovich
EL
Yakushenko
Elena Vladimirovna
EV
Kozlov
Vladimir Alexandrovich
VA
Sennikov
Sergei Vitalievich
SV
D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D005838:Genotype; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012800:Siberia; D055815:Young Adult
D020382:Interleukin-18