Preparation and biological properties of a melibiose binding lectin from Bauhinia variegata seeds.
A dimeric 64-kDa melibiose-binding lectin was isolated from the seeds of Bauhinia variegata. The isolation procedure comprised affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Mono Q, and gel filtration on Superdex 75. The lectin was adsorbed on the first two chromatographic media. Its hemagglutinating activity was stable after 30-min exposure to temperatures up to 70 degrees C. Since lectins may demonstrate biological activities such as antiproliferative, immunomodulatory, antifungal, antiviral, and HIV-1 reverse transcriptase inhibitory activities, the isolated lectin was tested for these activities. It was found that the lectin inhibited proliferation in hepatoma HepG2 cells and breast cancer MCF7 cells with an IC(50) of 1.4 microM and 0.18 microM, respectively. HIV-1 reverse transcriptase activity was inhibited with an IC(50) of 1.02 microM. The lectin and concanavalin A (Con A) evoked maximal mitogenic response from mouse splenocytes at similar concentrations, but the maximal response to B. variegata lectin was only 1/5 of that induced by Con A in magnitude. B. variegata lectin was devoid of antifungal activity.
Lin
Peng
P
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Ng
Tzi Bun
TB
D000595:Amino Acid Sequence; D036481:Bauhinia / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D008553:Melibiose / Q000378:metabolism*; D008938:Mitosis / Q000187:drug effects; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*
D037121:Plant Lectins; D018894:Reverse Transcriptase Inhibitors; D008553:Melibiose; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase