Modulation of transcriptional corepressor activity of prospero-related homeobox protein (Prox1) by SUMO modification.
Prospero-related homeobox protein (Prox1) plays essential roles in the development of many tissues and organs. In the present study, we show that Prox1 is modified by the small ubiquitin-like protein SUMO-1 in cultured cells. Mutation analysis identified at least four potential sumoylation sites within the repression domain of Prox1. Our data indicate that sumoylation of Prox1 reduces its interaction with HDAC3 and as a result downregulates its corepressor activity. These findings suggest that sumoylation may serve as a novel mechanism for the regulation of Prox1's corepressor activity.
Shan
Shi-Fang
SF
Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Wang
Lin-Fang
LF
Zhai
Jian-Wei
JW
Qin
Yi
Y
Ouyang
Hua-Fang
HF
Kong
Yu-Ying
YY
Liu
Jing
J
Wang
Yuan
Y
Xie
You-Hua
YH
D002460:Cell Line; D006655:Histone Deacetylases / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011499:Protein Processing, Post-Translational; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000378:metabolism*; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D000098551:Prospero-Related Homeobox 1 Protein; D000099186:Histone Deacetylase 3
D006655:Histone Deacetylases; D018398:Homeodomain Proteins; D012097:Repressor Proteins; D025842:SUMO-1 Protein; D025521:Tumor Suppressor Proteins; D000098551:Prospero-Related Homeobox 1 Protein; D000099186:Histone Deacetylase 3; C495501:SUMO1 protein, human