Role of apoptosis in erosive and reticular oral lichen planus exhibiting variable epithelial thickness.
Oral lichen planus (OLP) is a chronic inflammatory disease with different clinical types. Reticular and erosive forms are the most common. Although the cause of OLP remains speculative, many findings suggest auto-immune involvement, mediated by T lymphocytes against the basal keratinocytes. Inflammation, mechanical trauma or toxic agents can affect the epithelial homeostasia. Increased apoptosis may cause a decrease in epithelial thickness reflecting in the activity of the lesion. The objective of this study was to evaluate the occurrence of apoptosis and epithelial thickness in reticular and erosive forms of OLP. 15 samples of OLP each type (reticular and erosive) plus 10 of healthy mucosa were collected and processed. After morphometry, the apoptotic index and epithelial thickness were obtained. TUNEL and M30 CytoDEATH immunohistochemical assay were used to validate the morphologic criteria used. Apoptosis in the erosive OLP was significantly more intense than in the reticular type and both forms of OLP presented more apoptosis than the healthy oral mucosa. Healthy oral mucosa was thicker than both OLP forms and thicker in OLP reticular form than in the erosive one. The clinical differences between reticular and erosive forms of OLP are related to variations in epithelial thickness and in intensity of apoptosis.
Brant
Juliana M Caldeira
JM
Institute of Biological Sciences, Departament of General Pathology, Laboratory of Apoptosis, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. jumassote@yahoo.com.br
Vasconcelos
Anilton C
AC
Rodrigues
Luciana V
LV
D000911:Antibodies, Monoclonal; D017209:Apoptosis / Q000502:physiology*; D001485:Basement Membrane / Q000473:pathology; D002452:Cell Count; D002467:Cell Nucleus / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D006801:Humans; D006818:Hyalin / Q000737:chemistry; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D015603:Keratinocytes / Q000473:pathology; D017676:Lichen Planus, Oral / Q000145:classification / Q000473:pathology*; D009061:Mouth Mucosa / Q000473:pathology*; D012189:Retrospective Studies
D000911:Antibodies, Monoclonal