title: Myeloid cell differentiation arrest by miR-125b-1 in myelodysplastic syndrome and acute myeloid leukemia with the t(2;11)(p21;q23) translocation.
abstract: Most chromosomal translocations in myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML) involve oncogenes that are either up-regulated or form part of new chimeric genes. The t(2;11)(p21;q23) translocation has been cloned in 19 cases of MDS and AML. In addition to this, we have shown that this translocation is associated with a strong up-regulation of miR-125b (from 6- to 90-fold). In vitro experiments revealed that miR-125b was able to interfere with primary human CD34(+) cell differentiation, and also inhibited terminal (monocytic and granulocytic) differentiation in HL60 and NB4 leukemic cell lines. Therefore, miR-125b up-regulation may represent a new mechanism of myeloid cell transformation, and myeloid neoplasms carrying the t(2;11) translocation define a new clinicopathological entity.
authors.affiliation: Institut National de Santé et de Recherche Médicale, U563, Centre de Physiopathologie de Toulouse-Purpan, 31300 Toulouse, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Bousquet
authors.forename: Cathy
authors.initials: C
authors.lastname: Quelen
authors.forename: Roberto
authors.initials: R
authors.lastname: Rosati
authors.forename: Véronique
authors.initials: V
authors.lastname: Mansat-De Mas
authors.forename: Roberta
authors.initials: R
authors.lastname: La Starza
authors.forename: Christian
authors.initials: C
authors.lastname: Bastard
authors.forename: Eric
authors.initials: E
authors.lastname: Lippert
authors.forename: Pascaline
authors.initials: P
authors.lastname: Talmant
authors.forename: Marina
authors.initials: M
authors.lastname: Lafage-Pochitaloff
authors.forename: Dominique
authors.initials: D
authors.lastname: Leroux
authors.forename: Carine
authors.initials: C
authors.lastname: Gervais
authors.forename: Franck
authors.initials: F
authors.lastname: Viguié
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Lai
authors.forename: Christine
authors.initials: C
authors.lastname: Terre
authors.forename: Berna
authors.initials: B
authors.lastname: Beverlo
authors.forename: Costantina
authors.initials: C
authors.lastname: Sambani
authors.forename: Anne
authors.initials: A
authors.lastname: Hagemeijer
authors.forename: Peter
authors.initials: P
authors.lastname: Marynen
authors.forename: Georges
authors.initials: G
authors.lastname: Delsol
authors.forename: Nicole
authors.initials: N
authors.lastname: Dastugue
authors.forename: Cristina
authors.initials: C
authors.lastname: Mecucci
authors.forename: Pierre
authors.initials: P
authors.lastname: Brousset
mesh_terms: D002454:Cell Differentiation / Q000502:physiology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007558:Italy; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D035683:MicroRNAs / Q000378:metabolism*; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D022423:Myeloid Cells / Q000166:cytology* / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods; D014178:Translocation, Genetic / Q000235:genetics*; D015854:Up-Regulation / Q000502:physiology
chemical_list: D017931:DNA Primers; C531888:MIRN125 microRNA, human; D035683:MicroRNAs