title: Novel microchip for in situ TEM imaging of living organisms and bio-reactions in aqueous conditions.
abstract: A novel and disposable microchip (K-kit) with SiO(2) nano-membranes was developed and used as a specimen kit for in situ imaging of living organisms in an aqueous condition using transmission electron microscopy (TEM) without equipment modification. This K-kit enabled the successful TEM observation of living Escherichia coli cells and the tellurite reduction process in Klebsiella pneumoniae. The K. pneumoniae and Saccharomyces cerevisiae can stay alive in K-kit after continuous TEM imaging for up to 14 s and 42 s, respectively. Besides, different tellurite reduction profiles in cells grown in aerobic and anaerobic environments can be clearly revealed. These results demonstrate that the K-kit developed in this paper can be useful for observing living organisms and monitoring biological processes in situ.
authors.affiliation: Department of Materials Science and Engineering, National Tsing-Hua University, Hsinchu, Taiwan.
authors.forename: Kuo-Liang
authors.initials: KL
authors.lastname: Liu
authors.forename: Chien-Chen
authors.initials: CC
authors.lastname: Wu
authors.forename: Ying-Jung
authors.initials: YJ
authors.lastname: Huang
authors.forename: Hwei-Ling
authors.initials: HL
authors.lastname: Peng
authors.forename: Hwan-You
authors.initials: HY
authors.lastname: Chang
authors.forename: Pin
authors.initials: P
authors.lastname: Chang
authors.forename: Long
authors.initials: L
authors.lastname: Hsu
authors.forename: Tri-Rung
authors.initials: TR
authors.lastname: Yew
mesh_terms: D001419:Bacteria / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*; D002470:Cell Survival; D005240:Feasibility Studies; D005658:Fungi / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*; D007711:Klebsiella pneumoniae / Q000166:cytology / Q000378:metabolism; D046208:Microchip Analytical Procedures / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D055616:Microtechnology; D053758:Nanoparticles; D013691:Tellurium / Q000378:metabolism; D014867:Water
chemical_list: D014867:Water; C026660:tellurous acid; D013691:Tellurium