title: [GFP used as a reporter system for optimization of K562 cell transfection].
abstract: This study was aimed to obtain higher efficiency in gene transfection into K562 cells and to study the role of green fluorescence protein (GFP) as a reporter system. Transfection efficiencies with different methods including electroporation and lipofectamine 2000 transfection, were observed and calculated under fluorescent microscopy by using GFP as a reporter system. The results showed that the transfection efficiency with electroporation (10%) was higher than that with lipofectamine 2000 (1%). In conclusion, the electroporation is a more ideal method for introduction of foreign gene into K562 cells. GFP can be used as a reporter system for optimizing transfection of K562 cells.
authors.affiliation: Department of Hematology, PLA General Hospital, Beijing 100853, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Dou
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Chang
authors.initials: C
authors.lastname: Wang
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Kang
authors.forename: Yu
authors.initials: Y
authors.lastname: Jing
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Bo
authors.forename: Quan-Shun
authors.initials: QS
authors.lastname: Wang
authors.forename: Fang-Ding
authors.initials: FD
authors.lastname: Lou
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D018274:Electroporation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D008081:Liposomes; D014162:Transfection
chemical_list: D008081:Liposomes; D049452:Green Fluorescent Proteins