title: Naturally occurring feline leukemia virus subgroup A and B infections in urban domestic cats.
abstract: A nested-PCR (n-PCR) was used to detect feline leukemia virus (FeLV) proviral DNA in blood samples from 464 sick and 608 healthy domestic cats (Felis catus) selected by convenience, and a significantly high prevalence of FeLV infection was observed. n-PCR results revealed the presence of FeLV proviral DNA in 47.2 % of sick cats and 47.4 % of healthy cats. Phylogenetic analysis revealed that FeLV samples from healthy or sick cats were grouped into separate clades. We determined FeLV subgroups by an n-PCR based on the envelope (env) gene. The partial env gene of FeLV Minas Gerais (MG) samples were compared to various exogenous FeLV isolates and endogenous (enFeLV) provirus from the same region. FeLV-B MG samples were more similar to endogenous sequences and to natural FeLV-B isolates than to either FeLV-A or FeLV-C. The results revealed the circulation of FeLV-B in large populations of urban domestic cats in Brazil.
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Fabiana Magalhães
authors.initials: FM
authors.lastname: Coelho
authors.affiliation: Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Maria Rosa Quaresma
authors.initials: MRQ
authors.lastname: Bomfim
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Fabíola
authors.initials: F
authors.lastname: de Andrade Caxito
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Natália Almeida
authors.initials: NA
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Marcela Miranda
authors.initials: MM
authors.lastname: Luppi
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Érica Azevedo
authors.initials: ÉA
authors.lastname: Costa
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Maria Emilia
authors.initials: ME
authors.lastname: Oliveira
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Flávio Guimarães
authors.initials: FG
authors.lastname: Da Fonseca
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Resende
mesh_terms: D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015752:Genes, env; D006304:Health Status; D005256:Leukemia Virus, Feline / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016582:Leukemia, Feline / Q000821:virology*; D008019:Life Style; D008297:Male; D008969:Molecular Sequence Data; D011533:Proviruses / Q000302:isolation & purification; D012016:Reference Values; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014505:Urban Population; D014759:Viral Envelope Proteins / Q000737:chemistry
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins