title: Construction and characterization of a sugar beet (Beta vulgaris) fosmid library.
abstract: A sugar beet (Beta vulgaris) fosmid library from the doubled haploid accession KWS2320 encompassing 115 200 independent clones was constructed and characterized. The average insert size of the fosmid library was determined by pulsed field gel electrophoresis to be 39 kbp on average, thus representing 5.9-fold coverage of the sugar beet genome (758 Mbp). PCR screening of plate pools with primer pairs against nine sugar beet genes supported the insert size estimation. BLAST searches with 2951 fosmid end-sequences originating from 1510 clones (1536 clones attempted) revealed little contamination with organellar DNA (2.1% chloroplast DNA, 0.3% mitochondrial DNA). The sugar beet fosmid library will be integrated in the presently ongoing efforts to determine the sequence of the sugar beet genome. Fosmids will be publicly available in the format of plate pools and individual clones.
authors.affiliation: Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195 Berlin, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Lange
authors.forename: Daniela
authors.initials: D
authors.lastname: Holtgräwe
authors.forename: Britta
authors.initials: B
authors.lastname: Schulz
authors.forename: Bernd
authors.initials: B
authors.lastname: Weisshaar
authors.forename: Heinz
authors.initials: H
authors.lastname: Himmelbauer
mesh_terms: D001483:Base Sequence; D027461:Beta vulgaris / Q000235:genetics*; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018742:DNA, Chloroplast / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018742:DNA, Chloroplast; D004272:DNA, Mitochondrial; D018744:DNA, Plant