title: Polyphenol and phytosterol composition in an antibacterial extract from Rhizophora mangle L. bark.
abstract: Rhizophora mangle L. bark aqueous extract has antimicrobial, wound healing and antiulcerogenic properties. These properties could be associated with its chemical composition. To test this hypothesis, gravimetric, colorimetric, gas chromatography techniques were used to determine the preliminary chemical composition of this extract. Sephadex LH-20 Exclusion Chromatography was used by the fractionation of total extract and fractionation of low molecular weight polyphenols by liquid/liquid extraction. High Performance Liquid Chromatography was used to perform the composition in this low molecular weigh polyphenols fraction. The extract presented polyphenolic structures (54.78%) and other structural components (45.22%). Polymeric tannins were the major polyphenolic component (80%) and 20% were hydrolysable tannins. Epicatechin, catechin, chlorogenic acid, gallic acid and ellagic acid were monomeric structures determined in this extract. Phytosterols (0.0285%): stigmasterol, beta-sitosterol and campesterol were also present.
authors.affiliation: Department of Chemistry, Pharmacology and Toxicology; National Center of Agricultural Research, CENSA, Apdo. 10, CP 32 700, San José de Las Lajas, La Habana, Cuba. luzmaria@censa.edu.cu
authors.forename: Luz María
authors.initials: LM
authors.lastname: Sánchez Perera
authors.forename: Lino
authors.initials: L
authors.lastname: Varcalcel
authors.forename: Arturo
authors.initials: A
authors.lastname: Escobar
authors.forename: Mario
authors.initials: M
authors.lastname: Noa
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D010840:Phytosterols / Q000032:analysis*; D024301:Plant Bark / Q000737:chemistry*; D059808:Polyphenols; D015203:Reproducibility of Results; D043982:Rhizophoraceae / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D005419:Flavonoids; D010636:Phenols; D010840:Phytosterols; D059808:Polyphenols