[Comparison of IRIS IQ ELITE and microscopy for urinalysis and evaluation of performance in predicting outcome of urine cultures].
Since apprearance of reagent strip chemical determinations, different flow cytometers for urine sediments analysis appears (Sysmex UF-100, UF-50 and UF 1000). Recently has been introduced a new analyser (Iris IQ Elite) that analyses urine specimen by microscopy. Five hundred microscopic fields are photographed and analysed. Each particle of the microscopic field is separated and analysed with the software for recognition: Auto Particle Recognition (APR). In most of cases, automatic classification leads to correct results. For abnormal particles, the possibilities of visualisation by the operator permit us to avoid confusion between, for example: red cells and yeasts, spermatozoa and bacillus; that are identified as artefacts in flow cytometer. L'Iris IQ Elite presents a sensitivity of 68%, a specificity of 80%, a predictive positive value of 60% and a negative predictive value of 86% versus urine specimen culture. By combination of differents parameters given by the Iris machine, we can forget 43% of microscopic direct examination and predict the negativity of 32% of culture with a sensitivity and a negative predictive value of 100%.
Ledru
S
S
Laboratoire de microbiologie, Centre hospitalier Dr Schaffner, Lens. sledru@ch-lens.fr
Canonne
J-P
JP
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001331:Automation; D001437:Bacteriuria / Q000175:diagnosis; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008853:Microscopy / Q000379:methods*; D008875:Middle Aged; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012984:Software; D016482:Urinalysis / Q000295:instrumentation / Q000379:methods*; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology; D014556:Urine / Q000166:cytology / Q000382:microbiology