title: Proteomic analysis of the neuroprotective mechanisms of acupuncture treatment in a Parkinson's disease mouse model.
abstract: Acupuncture is frequently used as an alternative therapy for Parkinson's disease (PD), and it attenuates dopaminergic (DA) neurodegeneration in the substantia nigra (SN) in PD animal models. Using proteomic analysis, we investigated whether acupuncture alters protein expression in the SN to favor attenuation of neuronal degeneration. In C57BL/6 mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg/day), intraperitoneal (i.p.) for 5 days, 2 or 100 Hz electroacupuncture (EA) was applied at the effective and specific acupoint, GB34, once a day for 12 consecutive days from the first MPTP treatment. Both treatments in MPTP mice led to restoration of behavioral impairment and rescued tyrosine hydroxylase (TH)-positive DA neurodegeneration. Using peptide fingerprinting MS, we identified changes in 22 proteins in the SN following MPTP treatment, and nine of these proteins were normalized by EA. They were involved in cell death regulation, inflammation, or restoration from damage. The levels of cyclophilin A (CypA), which is a neuroprotective agent, were unchanged by MPTP treatment but were increased in MPTP-EA mice. These results suggest that acupoint GB34-specific EA changes protein expression profiles in the SN in favor of DA neuronal survival in MPTP-treated mice, and that EA treatment may be an effective therapy for PD patients.
authors.affiliation: Medical Science Research Center, Dongguk University, Gyeonggi-Do, Republic of Korea.
authors.forename: Songhee
authors.initials: S
authors.lastname: Jeon
authors.forename: Youn Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Seung-Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Woongjoon
authors.initials: W
authors.lastname: Moon
authors.forename: Younbyoung
authors.initials: Y
authors.lastname: Chae
authors.forename: Minjeong
authors.initials: M
authors.lastname: Kang
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Chung
authors.forename: Hyangsook
authors.initials: H
authors.lastname: Lee
authors.forename: Mi-Sook
authors.initials: MS
authors.lastname: Hong
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Tong H
authors.initials: TH
authors.lastname: Joh
authors.forename: Hyejung
authors.initials: H
authors.lastname: Lee
authors.forename: Hi-Joon
authors.initials: HJ
authors.lastname: Park
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000494:pharmacology*; D015669:Acupuncture Points; D000704:Analysis of Variance; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D021984:Cyclophilin A / Q000378:metabolism; D015259:Dopamine Agents / Q000494:pharmacology; D015671:Electroacupuncture; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D051379:Mice; D023421:Models, Animal; D009474:Neurons; D010300:Parkinson Disease / Q000628:therapy*; D010449:Peptide Mapping; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013378:Substantia Nigra / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D015259:Dopamine Agents; D020543:Proteome; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D014446:Tyrosine 3-Monooxygenase; D021984:Cyclophilin A