title: Vascular endothelial growth factor polymorphisms (-460C/T, +405G/C, and 936C/T) and endometriosis: their influence on vascular endothelial growth factor expression.
abstract: OBJECTIVE
  To analyze three functional vascular endothelial growth factor (VEGF) polymorphisms (-460C/T, +405G/C, and 936C/T) in women with and without endometriosis and their correlation with VEGF expression in endometrial tissue and peritoneal fluid (PF).
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  University-based hospital.
  
  
  METHODS
  One hundred eighty-six women with endometriosis and 180 controls without the disease.
  
  
  METHODS
  Endometrial biopsies were performed by aspiration and PF samples were obtained at laparoscopy.
  
  
  METHODS
  VEGF polymorphisms (-460C/T, +405G/C, and 936C/T) were determined using a polymerase chain reaction (PCR)-restriction fragment length polymorphism assay. Quantitative real-time reverse transcriptase (RT)-PCR assay was used to quantify VEGF-A messenger RNA (mRNA) and VEGF-A antigen levels were quantified by ELISA.
  
  
  RESULTS
  Patients with endometriosis showed a higher VEGF 936T allele frequency than controls. However, the distribution of genotypes and allele frequencies in the other two VEGF (-460C/T, +405G/C) polymorphisms was similar in the endometriosis and control groups. Endometrium and PF from women with endometriosis showed an increase in VEGF levels, but no association was found between the VEGF polymorphisms studied and VEGF expression in endometrial tissue and PF.
  
  
  CONCLUSIONS
  These findings suggest that the VEGF 936C/T polymorphism may be associated with the risk of endometriosis in a Caucasian population, but the increased VEGF levels observed in endometriosis do not appear to be associated with this polymorphism.
authors.affiliation: Research Center, Hospital Universitario La Fe, Valencia, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Cosín
authors.affiliation: Maternal Hospital, Hospital Universitario La Fe, Valencia, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Gilabert-Estellés
authors.affiliation: Research Center, Hospital Universitario La Fe, Valencia, Spain.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Ramón
authors.affiliation: Research Center, Hospital Universitario La Fe, Valencia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: España
authors.affiliation: Gynecology Service, Hospital Arnau de Vilanova, Valencia, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Gilabert
authors.affiliation: Maternal Hospital, Hospital Universitario La Fe, Valencia, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Romeu
authors.affiliation: Research Center, Hospital Universitario La Fe, Valencia, Spain. Electronic address: estelles_amp@gva.es.
authors.forename: Amparo
authors.initials: A
authors.lastname: Estellés
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression / Q000235:genetics; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D020125:Mutation, Missense / Q000502:physiology; D010532:Peritoneal Diseases / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D012307:Risk Factors; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism*; D055815:Young Adult
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A