title: Identification, characterization, and relatedness of luteovirus isolates from forage legumes.
abstract: ABSTRACT Virus isolates from forage legumes collected from eight different states were identified as luteoviruses closely related to soybean dwarf luteovirus dwarfing (SbDV-D) and yellowing (SbDV-Y) described in Japan. All isolates produced reddened leaf margins in subterranean clover and were transmitted in a persistent manner by Acrythosiphon pisum, but not by Aulacorthum solani. Specific monoclonal antibodies raised against SbDV-Y were differentially reactive with endemic isolates. Immunoblots probed with a SbDV-D polyclonal antiserum showed single 26-kDa coat protein bands, confirming close serological relatedness to SbDV. Analyses of genomic and subgenomic double-stranded RNAs and northern blot analyses confirmed genomic relatedness to SbDV. Based on our results, we conclude that the U.S. luteovirus isolates studied comprise a strain or strains of the soybean dwarf virus that have clovers as common hosts and the pea aphid as a common vector.
authors.forename: V D
authors.initials: VD
authors.lastname: Damsteegt
authors.forename: A L
authors.initials: AL
authors.lastname: Stone
authors.forename: A J
authors.initials: AJ
authors.lastname: Russo
authors.forename: D G
authors.initials: DG
authors.lastname: Luster
authors.forename: F E
authors.initials: FE
authors.lastname: Gildow
authors.forename: O P
authors.initials: OP
authors.lastname: Smith