Embryonic growth and mobilization of energy and material during incubation in the checkered keelback snake, Xenochrophis piscator.
We collected 20 checkered keelback snakes (Xenochrophis piscator) to study embryonic growth and mobilization of energy and material during incubation. Females laid eggs between late May and late June. The eggs were incubated at 27 degrees C (+/-0.3). One egg from each clutch was dissected at five-day intervals starting at oviposition. The mean incubation length at 27 degrees C was 48.9 days. We identified three phases of embryonic growth or yolk depletion in X. piscator. Phase 1, between oviposition and Day 20, was one of minimal transfer of energy and material from yolk to embryo. Phase 2, between Day 20 and Day 39-40, was characterized by increasingly rapid embryonic growth or yolk depletion. Phase 3, between Day 39-40 and hatching, was characterized by reduced embryonic growth or yolk depletion. Approximately 71% of dry mass, 53% of non-polar lipids and 66% of energy were transferred from the egg contents to the hatchling during incubation. Our data confirm that oviposition is not timed to coincide with the onset of rapid embryonic growth in oviparous squamate reptiles. The greater conversion efficiencies of energy and material from egg to hatchling in snakes can be attributed to their lower energetic costs of embryonic development and greater residual yolk sizes.
Lu
Hong-Liang
HL
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, Jiangsu, PR China.
Hu
Rui-Bin
RB
Ji
Xiang
X
D000818:Animals; D017812:Colubridae / Q000196:embryology* / Q000378:metabolism*; D004530:Egg Yolk / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D007185:Incubators; D050356:Lipid Metabolism; D008954:Models, Biological; D052287:Oviparity; D010058:Oviposition; D013997:Time Factors