Oxidative damage and antioxidant status in patients with cervical intraepithelial neoplasia and carcinoma of the cervix.
Free radicals that induced lipid peroxidation and DNA damage have been implicated in many diseases including cancer. Cellular antioxidant defense plays an important role in neoplastic disease to counteract oxidative damage. This study aims to investigate the status of oxidative damage by measuring plasma malondialdehyde (MDA) level and urinary 8-hydroxydeoxyguanosine (8-OHdG), and the level of antioxidant enzymes superoxide dismutase, glutathione peroxidase, and catalase in patients with cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC) of the cervix. Urinary 8-OHdG was measured by an enzyme-linked immunosorbent assay kit. MDA and antioxidant enzyme activities were determined by high-performance liquid chromatography and spectrophotometry, respectively. Eighty patients with CIN and SCC of the cervix were recruited and compared with normal controls. Urinary 8-OHdG/creatinine ratio did not show any significant changes in any disease status studied as compared with controls (P=0.803). Plasma MDA was found to be increased in CIN and SCC patients when compared with controls (P=0.002). Glutathione peroxidase activity was increased (P=0.0001) whereas superoxide dismutase and catalase activity was decreased (P=0.019 and 0.0001, respectively) in both CIN and SCC patients when compared with controls. Urinary 8-OHdG may not be a good marker for enhanced oxidative stress in cervical cancer. Oxidative damage as demonstrated by the level of MDA is markedly increased in CIN and SCC patients with changes of enzymatic antioxidants observed.
Looi
Mee-Lee
ML
Department of Biochemistry, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.
Mohd Dali
Ahmad Zailani Hatta
AZ
Md Ali
Siti Aishah
SA
Wan Ngah
Wan Zurinah
WZ
Mohd Yusof
Yasmin Anum
YA
D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D000975:Antioxidants / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000652:urine; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood / Q000378:metabolism; D004249:DNA Damage; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives / Q000652:urine; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000652:urine; D002578:Uterine Cervical Dysplasia / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000652:urine
D000975:Antioxidants; D006454:Hemoglobins; D008315:Malondialdehyde; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase