JADAS: a customizable automated data acquisition system and its application to ice-embedded single particles.
The JEOL Automated Data Acquisition System (JADAS) is a software system built for the latest generation of the JEOL Transmission Electron Microscopes. It is designed to partially or fully automate image acquisition for ice-embedded single particles under low dose conditions. Its built-in flexibility permits users to customize the order of various imaging operations. In this paper, we describe how JADAS is used to accurately locate and image suitable specimen areas on a grid of ice-embedded particles. We also demonstrate the utility of JADAS by imaging the epsilon 15 bacteriophage with the JEM3200FSC electron cryo-microscope, showing that sufficient images can be collected in a single 8h session to yield a subnanometer resolution structure which agrees with the previously determined structure.
Zhang
Junjie
J
Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
Nakamura
Natsuko
N
Shimizu
Yuko
Y
Liang
Nathan
N
Liu
Xiangan
X
Jakana
Joanita
J
Marsh
Michael P
MP
Booth
Christopher R
CR
Shinkawa
Takao
T
Nakata
Munetaka
M
Chiu
Wah
W
D000465:Algorithms; D001331:Automation; D001435:Bacteriophages / Q000648:ultrastructure*; D020285:Cryoelectron Microscopy; D007053:Ice; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D012984:Software
D007053:Ice