title: Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species.
abstract: OBJECTIVE
  To investigate the anti-oxidant properties of esculetin (6,7-dihydroxycoumarin) against H2O2-induced Chinese hamster lung fibroblast (V79-4) damage.
  
  
  METHODS
  The radical scavenging activity was assessed by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and intracellular reactive oxygen species (ROS). In addition, lipid peroxidation was assayed by the measure of related substances which react with thiobarbituric acid. The amount of carbonyl formation in protein was determined using a protein carbonyl ELISA kit. As well, cellular DNA damage was detected by Western blot and immunofluorescence image. Cell viability was assessed by 3-(4,5-dimethylthiazole-2- yl)-2,5-diphenyltetrazolium bromide assay.
  
  
  RESULTS
  Esculetin exhibited DPPH radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities. The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H2O2. Therefore, esculetin recovered cell viability exposed to H2O2.
  
  
  CONCLUSIONS
  Esculetin efficiently attenuated the oxidative stress induced cell damage via its anti-oxidant properties. As a result, esculetin may be useful in the development of functional food and raw materials of medicine.
authors.affiliation: School of Medicine and Institute of Medical Science, Cheju National University, Jeju-si, Republic of Korea.
authors.forename: So-hyung
authors.initials: SH
authors.lastname: Kim
authors.forename: Kyoung-ah
authors.initials: KA
authors.lastname: Kang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Mei-jing
authors.initials: MJ
authors.lastname: Piao
authors.forename: Dong-ok
authors.initials: DO
authors.lastname: Ko
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Wang
authors.forename: Sung-wook
authors.initials: SW
authors.lastname: Chae
authors.forename: Sam-sik
authors.initials: SS
authors.lastname: Kang
authors.forename: Keun-hwa
authors.initials: KH
authors.lastname: Lee
authors.forename: Hee-kyoung
authors.initials: HK
authors.lastname: Kang
authors.forename: Hyun-wook
authors.initials: HW
authors.lastname: Kang
authors.forename: Jin-won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D017665:Hydroxyl Radical / Q000737:chemistry; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates / Q000737:chemistry; D020011:Protective Agents; D050050:Protein Carbonylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*; D014468:Umbelliferones / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D016877:Oxidants; D010851:Picrates; D020011:Protective Agents; D017382:Reactive Oxygen Species; D014468:Umbelliferones; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; C007628:esculetin