title: Effect of vitamin C administration on neutrophil apoptosis in septic patients after abdominal surgery.
abstract: OBJECTIVE
  To investigate the effect of parenteral administration of vitamin C on neutrophil apoptosis by determining Fas receptor expression and caspase-3, poly (ADP-ribose) polymerase (PARP), and Bcl-2 levels in neutrophils from septic abdominal surgery patients.
  
  
  METHODS
  Twenty septic abdominal surgery patients were studied in a prospective, randomized, double-blinded clinical trial. A group of healthy volunteers (n = 10) constituted a reference group for baseline parameter values. The patients were randomly assigned to a vitamin C-treated (n = 10) or placebo-treated (n = 10) group. For a 6-d period from 12 h post-surgery, the vitamin C group received 450 mg/d of the vitamin in 3 doses and the placebo group an identical administration of 5% dextrose. Early-morning peripheral blood samples were obtained daily from 24 h after vitamin C administration until d 6 post-surgery (T1d-T6d).
  
  
  RESULTS
  Vitamin C group showed a nonsignificant reduction in Fas (CD95) expression on CD15-positive peripheral blood neutrophils, significantly decreased caspase-3, and PARP levels (caspase-3: T4d: P < 0.05, T5d: P < 0.05, T6d P < 0.01; and PARP: T3d: P < 0.05, T4d: P < 0.05, T6d: P < 0.05), and significantly increased Bcl-2 levels (T3d: P = 0.001) versus placebo group.
  
  
  CONCLUSIONS
  Postoperative vitamin C treatment of septic abdominal surgery patients exerts an antiapoptotic effect on peripheral blood neutrophils, reducing caspase-3 and PARP levels, and increasing Bcl-2 levels. However, these antiapoptotic effects are not maintained at all time points.
authors.affiliation: Experimental Surgery Research Unit, Virgen de las Nieves University Hospital, Granada, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Ferrón-Celma
authors.forename: Alfonso
authors.initials: A
authors.lastname: Mansilla
authors.forename: Laila
authors.initials: L
authors.lastname: Hassan
authors.forename: Ana
authors.initials: A
authors.lastname: Garcia-Navarro
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Comino
authors.forename: Pablo
authors.initials: P
authors.lastname: Bueno
authors.forename: Jose-Antonio
authors.initials: JA
authors.lastname: Ferrón
mesh_terms: D000368:Aged; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001205:Ascorbic Acid / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D013505:Digestive System Surgical Procedures; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011182:Postoperative Care; D011446:Prospective Studies; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D018805:Sepsis / Q000601:surgery*; D019014:fas Receptor / Q000378:metabolism
chemical_list: D000975:Antioxidants; D019253:Proto-Oncogene Proteins c-bcl-2; D019014:fas Receptor; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3; D001205:Ascorbic Acid