title: Identification, expression and antigenic analysis of recombinant hemagglutinin proteins of canine distemper virus.
abstract: Canine distemper (CD) is a widely distributed disease of dogs, caused by the canine distemper virus (CDV). In the present study, the gene encoding the hemagglutinin (H) protein of a CDV isolate from central Taiwan was sequenced and compared with other strains. Sequence variations were noticed in the H gene from the field CDV strain that had previously been implicated in the increasing incidence of CD. To establish a serology-based diagnostic test, the full-length H protein, as well as five deletion mutants of a recombinant H protein of the local isolate, were produced using an E. coli expression system. Three truncated recombinant proteins with relatively high expression levels, designated HM3, HM4 and HM5, were used as antigens to examine their reactivity with canine sera. By using three negative sera and 17 CD-positive sera, the high specificity of recombinant H proteins was observed by ELISA. In addition, immunoblotting demonstrated that all three purified recombinant proteins exhibit an antigenic property recognized by the serum of a CD-suspected dog.
authors.affiliation: Department of Veterinary Medicine College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Kun-Wei
authors.initials: KW
authors.lastname: Chan
authors.forename: Hsien-Hua
authors.initials: HH
authors.lastname: Hsieh
authors.forename: Hsien-Chi
authors.initials: HC
authors.lastname: Wang
authors.forename: Ya-Jane
authors.initials: YJ
authors.lastname: Lee
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Sung
authors.forename: Min-Liang
authors.initials: ML
authors.lastname: Wong
authors.forename: Wei-Li
authors.initials: WL
authors.lastname: Hsu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004216:Distemper / Q000175:diagnosis* / Q000821:virology; D004217:Distemper Virus, Canine / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D017353:Gene Deletion; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D011994:Recombinant Proteins