Unravelling the nuclear matrix proteome.
The nuclear matrix (NM) model posits the presence of a protein/RNA scaffold that spans the mammalian nucleus. The NM proteins are involved in basic nuclear function and are a promising source of protein biomarkers for cancer. Importantly, the NM proteome is operationally defined as the proteins from cells and tissue that are extracted following a specific biochemical protocol; in brief, the soluble proteins and lipids, cytoskeleton, and chromatin elements are removed in a sequential fashion, leaving behind the proteins that compose the NM. So far, the NM has not been sufficiently verified as a biological entity and only preliminary at the molecular level. Here, we argue for a combined effort of proteomics, immunodetection and microscopy to unravel the composition and structure of the NM.
Albrethsen
Jakob
J
OncoProteomics Laboratory, CCA 1-60, Department Medical Oncology, VUmc-Cancer Center Amsterdam, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands.
Knol
Jaco C
JC
Jimenez
Connie R
CR
D000818:Animals; D006801:Humans; D046529:Microscopy, Electron, Transmission; D009369:Neoplasms / Q000378:metabolism; D015530:Nuclear Matrix / Q000378:metabolism* / Q000648:ultrastructure; D034521:Nuclear Matrix-Associated Proteins / Q000032:analysis* / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*
D034521:Nuclear Matrix-Associated Proteins; D020543:Proteome