Significant elevation of plasma matrix metalloproteinase-9 level and its ratio to matrix metalloproteinase-2 in patients with pelvic inflammatory disease.
OBJECTIVE
To detect the expression of plasma matrix metalloproteinase-2 (MMP-2) and MMP-9, and MMP-9:MMP-2 ratio in patients with pelvic inflammatory disease (PID).


METHODS
A consecutive study with approximate 1:2 case-to-control ratio.


METHODS
University hospital.


METHODS
Forty-seven patients with PID and 80 healthy women.


METHODS
Collected blood specimens of patients with PID before and after treatment.


METHODS
ELISA and gelatin zymography were used to measure the expression of plasma MMP-2 and MMP-9.


RESULTS
The level of plasma MMP-9 or MMP-9:MMP-2 ratio was elevated in patients with PID compared with healthy controls and decreased significantly after treatment. The activity of MMP-9, but not MMP-2, tended to be higher in 47 patients with PID before the treatment compared with that after the treatment. Pretreatment plasma MMP-9 or MMP-9:MMP-2 ratio was significantly correlated with white blood cell (WBC) and neutrophil counts. As prediction markers for PID, the sensitivities of MMP-9 and MMP-9:MMP-2 ratio were 76.6% and 76.6%, whereas the negative predictive values were 82.5% and 83.3%.


CONCLUSIONS
Level of plasma MMP-9 and MMP-9:MMP-2 ratio may act as prediction markers for PID. In patients with PID, 80% have a plasma MMP-9 level higher than 115 ng/mL or a MMP-9:MMP-2 ratio higher than 2.15.
Wang
Po-Hui
PH
Department of Obstetrics and Gynecology, Chung Shan Medical University, Taichung, Taiwan.
Tsai
Hsiu-Ting
HT
Tee
Yi-Torng
YT
Lin
Long-Yau
LY
Yang
Shun-Fa
SF
Hsieh
Yih-Shou
YS
D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D009504:Neutrophils / Q000473:pathology; D000292:Pelvic Inflammatory Disease / Q000097:blood* / Q000175:diagnosis / Q000473:pathology / Q000628:therapy; D011237:Predictive Value of Tests; D011379:Prognosis; D012372:ROC Curve; D015854:Up-Regulation; D055815:Young Adult
D015415:Biomarkers; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9