The dynamic of lipid oxidation in human myotubes.
Both endogenous and exogenous lipid levels may be regulators of total lipid oxidation in skeletal muscles. We studied the dynamics of lipid oxidation in human myotubes established from healthy, lean subjects exposed to acutely and chronically increased palmitate concentrations. The intramyocellular triacylglycerol content increased with chronic palmitate exposure. Both, ectopically increased intracellular and extracellular lipid levels were simultaneously oxidized and could partly suppress each other's oxidation. Overall, the highest acute palmitate treatments stimulated fatty acid oxidation whilst the highest chronic treatments decreased total lipid oxidation. Intracellular lipids showed a more complete oxidation than exogenous lipids. Endogenous lipids reduced insulin-mediated glucose oxidation. Thus, both endogenous and exogenous lipid concentrations regulated each other's oxidation and total lipid oxidation in human myotubes. A reduced exogenous lipid oxidation, secondary to increased triacylglycerol levels, may redirect free fatty acids into esterification and oxidation from intracellular stores, thereby protecting myotubes from FFA lipotoxic effects.
Gaster
Michael
M
KMEB, Dept. of Endocrinology, Odense University Hospital, DK-5000 Odense, Denmark. Michael.Gaster@ouh.fyns-amt.dk
D002478:Cells, Cultured; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000378:metabolism / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D019308:Palmitic Acid