Chiral separation of raltitrexed by cyclodextrin-modified micellar electrokinetic chromatography.
A rapid and effective method was developed for the chiral separation of raltitrexed (RD) enantiomers by carboxymethyl-beta-cyclodextrin (CM-beta-CD)-modified micellar electrokinetic chromatography (MEKC). Optimization of conditions including the type and concentration of the chiral selector, concentration of sodium dodecyl sulfate (SDS), pH and concentration of the background electrolyte (BGE), capillary temperature, and applied voltage was investigated. The enantiomers of raltitrexed could be separated with satisfactory resolution and linear response by using 75 mM Tris-phosphate at pH 8.0 containing 30 mM SDS and 8 mM CM-beta-CD as buffer system. Furthermore, the usefulness of this method was demonstrated in a purity test of a real synthetic drug sample.
Liu
Yi
Y
Institute of Analytical Chemistry, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
Fu
Xiaofang
X
Ma
Chao
C
Zhong
Jiasheng
J
Liao
Yiping
Y
Liu
Huwei
H
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004563:Electrochemistry; D004573:Electrolytes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008968:Molecular Conformation; D011799:Quinazolines / Q000737:chemistry* / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013237:Stereoisomerism; D013696:Temperature; D013876:Thiophenes / Q000737:chemistry* / Q000302:isolation & purification*; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry*
D004573:Electrolytes; D011799:Quinazolines; D013876:Thiophenes; D047392:beta-Cyclodextrins; C441457:carboxymethyl-beta-cyclodextrin; D012967:Sodium Dodecyl Sulfate; C068874:raltitrexed