[GFP used as a reporter system for optimization of K562 cell transfection].
This study was aimed to obtain higher efficiency in gene transfection into K562 cells and to study the role of green fluorescence protein (GFP) as a reporter system. Transfection efficiencies with different methods including electroporation and lipofectamine 2000 transfection, were observed and calculated under fluorescent microscopy by using GFP as a reporter system. The results showed that the transfection efficiency with electroporation (10%) was higher than that with lipofectamine 2000 (1%). In conclusion, the electroporation is a more ideal method for introduction of foreign gene into K562 cells. GFP can be used as a reporter system for optimizing transfection of K562 cells.
Dou
Li-Ping
LP
Department of Hematology, PLA General Hospital, Beijing 100853, China.
Liu
Jun-Hua
JH
Wang
Chang
C
Liu
Jing-Hua
JH
Kang
Hui-Yuan
HY
Jing
Yu
Y
Zhao
Yu
Y
Bo
Jian
J
Wang
Quan-Shun
QS
Lou
Fang-Ding
FD
Yu
Li
L
D018274:Electroporation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D008081:Liposomes; D014162:Transfection
D008081:Liposomes; D049452:Green Fluorescent Proteins