title: [Development of a protocol on pulsed field gel electrophoresis analysis for Streptococcus suis].
abstract: OBJECTIVE
  To develop a PFGE protocol for Streptococcus suis.
  
  
  METHODS
  We developed and optimized a PFGE protocol for S. suis, in terms of plug preparation, choice of restriction endonucleases and optimized electrophoresis parameters. By analyzing the genome sequences of S. suis P1/7 with Mapdraw of DNAStar, we found three restriction enzymes, Swa I, Sma I and Apa I, were more suitable than others.
  
  
  RESULTS
  Analysis of 100 isolates of S. suis including 34 of 35 serotypes identified, 59, 53 and 43 patterns were obtained from Swa I, Sma I and Apa I restriction, respectively. The enzyme Swa I had the greatest power for discrimination ability.
  
  
  CONCLUSIONS
  By optimization of the protocol at various conditions, a rapid, reproducible, economic and practical PFGE method for S. suis was developed.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Chang-Yun
authors.initials: CY
authors.lastname: Ye
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Xu
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Cui
authors.forename: Huai-Qi
authors.initials: HQ
authors.lastname: Jing
authors.forename: Dong
authors.initials: D
authors.lastname: Jin
authors.forename: Hua-Mao
authors.initials: HM
authors.lastname: Du
authors.forename: Shou-Yin
authors.initials: SY
authors.lastname: Zhang
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Bai
authors.forename: Ai-Lan
authors.initials: AL
authors.lastname: Zhao
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Xu
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D012703:Serotyping; D017011:Streptococcus suis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine
chemical_list: D004269:DNA, Bacterial