Photoswitchable nanoparticles enable high-resolution cell imaging: PULSAR microscopy.
Beyond-diffraction-limit optical imaging of cells will reveal biological mechanisms, cellular structures, and physiological processes in nanometer scale. Harnessing the photoswitching properties of spiropyran fluorophores, we achieved nanoresolution fluorescence imaging using photoactuated unimolecular logical switching attained reconstruction (PULSAR) microscopy. The PULSAR microscope successfully resolved nanostructures and subcellular organelles when the photoswitchable nanoparticles containing spiropyran dyes were used as fluorescent probes.
Hu
Dehong
D
Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
Tian
Zhiyuan
Z
Wu
Wuwei
W
Wan
Wei
W
Li
Alexander D Q
AD
D001578:Benzopyrans / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007211:Indoles / Q000737:chemistry; D008853:Microscopy; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D009574:Nitro Compounds / Q000737:chemistry; D010777:Photochemistry
D001578:Benzopyrans; D007211:Indoles; D009574:Nitro Compounds; C088184:spiropyran