Isolation and characterization of PERV-C env from domestic pig in Korea.
Clone PERV-C (A3) env was isolated from the genomic DNA of domestic pig (Sus scrofa domesticus) in Korea to investigate the molecular properties of PERV-C. The nucleic acid homologies between the PERV-MSL (type C) reference and the PERV-C(A3) clone was 99% for env, but a single base pair deletion was found in the transmembrane (TM) region of the env open reading frame. To examine the functional characteristics of truncated PERV-C env, we constructed a replication-incompetent retroviral vector by replacing the env gene of the pCL-Eco retrovirus vector with PERV-C env. A retroviral vector bearing PERV-C/A chimeric envelopes was also created to complement the TM defect. Our results indicated that truncated PERV-C env was not infectious in human cells as expected. Interestingly, however, the vector with the PERV-C/A envelope was able to infect 293 cells. This observation suggests that recombination within PERV-C TM could render PERV-C infectious in humans. To further characterize PERV-C/A envelopes, we constructed an infectious molecular clone by using a PCR-based technique. This infectious molecular clone will be useful to examine more specific regions that are critical for human cell tropism.
Park
Sung-Han
SH
Department of Microbiology and Institute of Basic Science, Dankook University, Cheonan 330-714, Korea.
Bae
Eun Hye
EH
Park
Sang Min
SM
Park
Jin Woo
JW
Lim
Mi Suk
MS
Jung
Yong-Tae
YT
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004197:Disease Reservoirs / Q000821:virology; D006801:Humans; D007723:Korea; D008969:Molecular Sequence Data; D017354:Point Mutation; D011995:Recombination, Genetic; D012190:Retroviridae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012192:Retroviridae Infections / Q000821:virology*; D016415:Sequence Alignment; D034421:Sus scrofa / Q000821:virology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D014759:Viral Envelope Proteins