title: Protocols for nuclei isolation and nuclear protein extraction from the resurrection plant Xerophyta viscosa for proteomic studies.
abstract: The plant nucleus is an important subcellular organelle but the isolation of pure and enriched nuclei from plants and subsequent extraction of nuclear proteins for proteomic studies is challenging. Here, we present protocols for nuclei isolation and nuclear protein extraction from the resurrection plant, Xerophyta viscosa, and show optimization and modification of the most critical steps.
authors.affiliation: Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch 7701, South Africa.
authors.forename: Kamal Omer
authors.initials: KO
authors.lastname: Abdalla
authors.forename: Jennifer Ann
authors.initials: JA
authors.lastname: Thomson
authors.forename: Muhammad Suhail
authors.initials: MS
authors.lastname: Rafudeen
mesh_terms: D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus / Q000737:chemistry* / Q000378:metabolism; D032181:Craterostigma / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D009687:Nuclear Proteins / Q000302:isolation & purification*; D010940:Plant Proteins / Q000302:isolation & purification*; D040901:Proteomics
chemical_list: D009687:Nuclear Proteins; D010940:Plant Proteins