A computerized analysis of the entire retinal ganglion cell population and its spatial distribution in adult rats.
In adult albino (SD) and pigmented (PVG) rats the entire population of retinal ganglion cells (RGCs) was quantified and their spatial distribution analyzed using a computerized technique. RGCs were back-labelled from the optic nerves (ON) or the superior colliculi (SCi) with Fluorogold (FG). Numbers of RGCs labelled from the ON [SD: 82,818+/-3,949, n=27; PVG: 89,241+/-3,576, n=6) were comparable to those labelled from the SCi [SD: 81,486+/-4,340, n=37; PVG: 87,229+/-3,199; n=59]. Detailed methodology to provide cell density information at small scales demonstrated the presence of a horizontal region in the dorsal retina with highest densities, resembling a visual streak.
Salinas-Navarro
M
M
Laboratorio de Oftalmología Experimental, Facultad de Medicina, Universidad de Murcia, E-30100 Murcia, Spain.
Mayor-Torroglosa
S
S
Jiménez-López
M
M
Avilés-Trigueros
M
M
Holmes
T M
TM
Lund
R D
RD
Villegas-Pérez
M P
MP
Vidal-Sanz
M
M
D000818:Animals; D002452:Cell Count; D001330:Electronic Data Processing; D005260:Female; D005456:Fluorescent Dyes; D008297:Male; D008856:Microscopy, Fluorescence; D009900:Optic Nerve; D051381:Rats; D011914:Rats, Inbred BN; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012165:Retinal Ganglion Cells / Q000166:cytology*; D013045:Species Specificity; D013477:Superior Colliculi
D005456:Fluorescent Dyes