Engineering of the alpha-amylase from Geobacillus stearothermophilus US100 for detergent incorporation.
AmyUS100DeltaIG is a variant of the most thermoactive and thermostable maltohexaose forming alpha-amylase produced by Geobacillus stearothermophilus sp.US100. This enzyme which was designed to improve the thermostability of the wild-type enzyme has acquired a very high resistance to chelator agents. According to modeling structural studies and with the aim of enhancing its resistance towards chemical oxidation, a mutant (AmyUS100DeltaIG/M197A) was created by substituting methionine 197 to alanine. The catalytic proprieties of the resulting mutant show alterations in the specific activity and the profile of starch hydrolysis. Interestingly, AmyUS100DeltaIG/M197A displayed the highest resistance to oxidation compared to the AmyUS100DeltaIG and to Termamyl300, the well-known commercial amylase used in detergent. Further, performance of the engineered alpha-amylase was estimated in the presence of commonly used detergent compounds and a wide range of commercial detergent (liquid and solid). These studies indicated a high compatibility and performance of AmyUS100DeltaIG/M197A, suggesting its potential application in detergent industry.
Khemakhem
Bassem
B
Centre de Biotechnologie de Sfax B.P. "K" 3038 Sfax, Tunisie.
Ali
Mamdouh Ben
MB
Aghajari
Nushin
N
Juy
Michel
M
Haser
Richard
R
Bejar
Samir
S
D000409:Alanine / Q000737:chemistry / Q000235:genetics; D019943:Amino Acid Substitution; D001407:Bacillus / Q000201:enzymology*; D002384:Catalysis; D003902:Detergents / Q000737:chemistry*; D004795:Enzyme Stability / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D008715:Methionine / Q000737:chemistry / Q000235:genetics; D016296:Mutagenesis; D009844:Oligosaccharides / Q000096:biosynthesis; D010084:Oxidation-Reduction; D011487:Protein Conformation; D015202:Protein Engineering; D013329:Structure-Activity Relationship; D000516:alpha-Amylases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*
D003902:Detergents; D009844:Oligosaccharides; C016549:maltohexaose; D008715:Methionine; D000516:alpha-Amylases; D000409:Alanine