Effect of berberine on the antioxidant status, ultrastructural modifications and protein bound carbohydrates in azoxymethane-induced colon cancer in rats.
Chemically induced carcinogenesis models in the rat are widely used for studying the biology of cancer and for developing and evaluating cancer prevention strategies. The azoxymethane(AOM)-induced rat colon tumor is a valuable tool for studying the interaction between tumor development and exogenous factors. Malignant conditions are characterized by enhanced levels of lipid peroxidative products, protein bound carbohydrates indicative of membrane damage and an ineffective antioxidant scavenging system. In the present study, AOM-induced rat showed lipid peroxidative damage, alterations in the membrane glycoprotein component and antioxidant defense system, along with ultrastructural changes like disruption in goblet cell and its membrane, swollen mitochondria with cristae dispersed, elongated endoplasmic reticulum and other features of neoplastic invasion. Berberine significantly attenuated the increases in lipid peroxidation, protein bound carbohydrates and enhanced the antioxidative status. The present study suggests that berberine prevents the appearance of malignant morphology and ultrastructural changes of AOM induced cancer by producing apoptosis like changes. Thus berberine inhibits neoplastic transformation by the induction of antioxidant defence system and ability to induce apoptotic like changes--thus elucidating its anti cancer role.
Thirupurasundari
Chandragiri Janakiraman
CJ
Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India. cjlaka26@rediffmail.com
Padmini
Ramakrishnan
R
Devaraj
Sivasithambaram Niranjali
SN
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001397:Azoxymethane / Q000633:toxicity*; D001599:Berberine / Q000494:pharmacology*; D001798:Blood Proteins / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002273:Carcinogens / Q000633:toxicity*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000648:ultrastructure; D015227:Lipid Peroxidation; D008297:Male; D046529:Microscopy, Electron, Transmission; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D001798:Blood Proteins; D002273:Carcinogens; D001599:Berberine; D001397:Azoxymethane