Hydroxyethylcellulose-graft-poly (N,N-dimethylacrylamide) copolymer as a multifunctional separation medium for CE.
A new multifunctional separation medium, hydroxyethylcellulose-graft-poly (N,N-dimethylacrylamide) copolymer synthesized in our laboratory for application in both basic protein separation and dsDNA separation by CE, is presented in this paper. As a noncovalent coating, this medium showed a powerful capability in resisting basic protein adsorption. Highly efficient and rapid protein separation had been obtained at four different pH values. Meanwhile, the 11 fragments of the dsDNA sample could be baseline separated using this grafted copolymer as sieving matrix at an appropriate concentration.
Peng
Shuhua
S
Department of Polymer Science and Engineering, CAS Key Laboratory of Soft Matter Chemistry, University of Science and Technology of China, Hefei, PR China.
Shi
Ronghua
R
Yang
Runmiao
R
Zhou
Dan
D
Wang
Yanmei
Y
D000178:Acrylamides; D002482:Cellulose / Q000031:analogs & derivatives*; D045304:Cytochromes c / Q000032:analysis; D004247:DNA / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000032:analysis; D012259:Ribonuclease, Pancreatic / Q000032:analysis
D000178:Acrylamides; C538719:hydroxyethylcelluose-graft-poly(N,N-dimethylacrylamide); D002482:Cellulose; D045304:Cytochromes c; D004247:DNA; D012259:Ribonuclease, Pancreatic; D009113:Muramidase