title: Mineralocorticoids restore quiescent morphology and reduce VEGF receptor expression in inflamed choroidal endothelial cells in vitro.
abstract: OBJECTIVE
  While the glucocorticoid triamcinolone acetonide (9alpha-fluoro-16alpha-hydroxyprednisolone, TA) has been widely administered as a treatment of ocular inflammation, mineralocorticoids have not been tested for their efficacy.
  
  
  METHODS
  We assessed cellular morphology and actin distribution by immunomicroscopy and light microscopy, membrane permeability with transendothelial resistance and cell surface vascular endothelial growth factor receptor-1 (VEGF-R1) expression by flow cytometry.
  
  
  RESULTS
  Fludrocortisone acetate was more effective than TA in restoring quiescent morphology and reducing membrane permeability in phorbol-12-myristate-acetate (PMA)-stimulated choroidal endothelial cells (CECs). Each of the corticosteroids inhibited VEGF-R1 cell surface expression in PMA-responsive CECs.
  
  
  CONCLUSIONS
  Mineralocorticoids may be of potential use in reducing vascular permeability in ocular disease.
authors.affiliation: School of Animal Biology, University of Western Australia, Crawley, W.A., Australia. melfitz1@cyllene.uwa.edu.au
authors.forename: Melinda
authors.initials: M
authors.lastname: Fitzgerald
authors.forename: Lauren
authors.initials: L
authors.lastname: Evill
authors.forename: Kelly
authors.initials: K
authors.lastname: Banz
authors.forename: Simon
authors.initials: S
authors.lastname: Carroll
authors.forename: Jennifer
authors.initials: J
authors.lastname: Rodger
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D045744:Cell Line, Tumor; D048429:Cell Size / Q000187:drug effects; D002829:Choroid / Q000166:cytology*; D003350:Cortodoxone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects*; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D008253:Macaca mulatta; D008901:Mineralocorticoids / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D040262:Receptors, Vascular Endothelial Growth Factor / Q000235:genetics / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014222:Triamcinolone Acetonide / Q000494:pharmacology
chemical_list: D000199:Actins; D008901:Mineralocorticoids; D012333:RNA, Messenger; D040262:Receptors, Vascular Endothelial Growth Factor; D014222:Triamcinolone Acetonide; D013755:Tetradecanoylphorbol Acetate; D003350:Cortodoxone