title: Mitochondrial DNA sequence variation among geographical isolates of Opisthorchis viverrini in Thailand and Lao PDR, and phylogenetic relationships with other trematodes.
abstract: The present study compared the genetic variation among 14 different geographical isolates of Opisthorchis viverrini sensu lato from Thailand and Lao PDR using sequence data for 2 mitochondrial DNA genes, the subunit 1 of NADH dehydrogenase gene (nad1) and cytochrome c oxidase gene (cox1). Four different nad1 haplotypes were detected among isolates, all of which were identical at the amino acid sequence level. Nucleotide sequence variation among 14 isolates ranged from 0 to 0.3% for nad1. Two different cox1 haplotypes were detected among isolates. These two haplotypes differed at 2 nucleotide positions, one of which resulted in a change in the amino acid sequence. Nucleotide sequence variation among isolates for cox1 ranged from 0 to 0.5%. Comparison of cox1 sequences of O. viverrini to those of other trematodes revealed nucleotide differences of 13-31%. A phylogenetic analysis of the cox1 sequence data revealed strong statistical support for a clade containing O. viverrini and 2 other species of opisthorchid trematodes; O. felineus and Clonorchis sinsensis.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Saijuntha
authors.forename: P
authors.initials: P
authors.lastname: Sithithaworn
authors.forename: S
authors.initials: S
authors.lastname: Wongkham
authors.forename: T
authors.initials: T
authors.lastname: Laha
authors.forename: N B
authors.initials: NB
authors.lastname: Chilton
authors.forename: T N
authors.initials: TN
authors.lastname: Petney
authors.forename: M
authors.initials: M
authors.lastname: Barton
authors.forename: R H
authors.initials: RH
authors.lastname: Andrews
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth; D004272:DNA, Mitochondrial / Q000235:genetics*; D003710:Demography; D005786:Gene Expression Regulation; D014644:Genetic Variation; D007812:Laos; D009891:Opisthorchis / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D013785:Thailand
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial