Antioxidant and oxidative stress status in type 2 diabetes and diabetic foot ulcer.
OBJECTIVE
Oxidative stress (OS) has been implicated in the aetiology and progression of diabetic complications including diabetic foot ulcer. In this study, the levels of lipid peroxides (LPO) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) as well as the enzymatic antioxidant activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in type 2 diabetes mellitus and diabetic foot ulcer subjects were assessed and compared with apparently healthy normal subjects to understand the involvement of OS in the subjects.


METHODS
The above-mentioned OS markers were measured in 50 subjects for each of the following groups: type 2 diabetes mellitus (DM), diabetic foot ulcer (DF) and non-diabetic control (NC).


RESULTS
Significant elevated values of LPO (39.86%) and 8-OHdG (45.53%) were found in DM subjects compared with the NC subjects. This increase in both parameters was greater for DF subjects: 80.23% and 53.91% respectively. SOD activities were significantly reduced in DM (14.82%) and DF (4.09%) subjects in contrast with elevated activities of GPx observed in DM (21.87%) and DF (20.94%) subjects. Glycated haemoglobin/fasting plasma glucose (HbA1c/FPG) correlated positively with LPO, 8-OHdG and GPx, whereas a negative correlation was observed for SOD.


CONCLUSIONS
Increased oxidation subsequent to diabetic conditions induces an over-expression of GPx activity suggesting a compensatory mechanism by the body to prevent further tissue damage in the subjects.
Bolajoko
Elizabeth Bosede
EB
Department of Chemical Pathology, University of Limpopo, Medunsa Campus, GaRankuwa.
Mossanda
Kensese Sontin
KS
Adeniyi
Francis
F
Akinosun
Olubayo
O
Fasanmade
Adesoji
A
Moropane
Mpho
M
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D016022:Case-Control Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D017719:Diabetic Foot / Q000097:blood* / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D006943:Hyperglycemia / Q000150:complications; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012307:Risk Factors; D013482:Superoxide Dismutase / Q000097:blood
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000975:Antioxidants; D001786:Blood Glucose; D003849:Deoxyguanosine; D005979:Glutathione Peroxidase; D006442:Glycated Hemoglobin; D008054:Lipid Peroxides; D013482:Superoxide Dismutase