[Loss of heterozygosity on chromosome 3p in thyroid tumors].
OBJECTIVE
To study the loss of heterozygosity (LOH) on chromosome 3p in thyroid tumors.


METHODS
LOH at 11 microsatellite loci was analyzed in 74 cases of thyroid tumors (including 20 follicular adenomas, 24 follicular thyroid carcinomas and 30 papillary thyroid carcinomas) by polymerase chain reaction and silver stain.


RESULTS
LOH on chromosome 3p was detected in 71% of follicular thyroid carcinoma (17/24), 30% of the papillary thyroid carcinoma (9/30) and 10% of the follicular adenoma (2/20) case. Two minimal common deleted regions (CDR) (3p26-pter and 3p14.2-3p22) involving significant sites of LOH has identified in follicular thyroid carcinoma. There was also one CDR (3p25. 2-26.1) in papillary thyroid carcinoma.


CONCLUSIONS
LOH is more frequently identified in follicular thyroid carcinoma than in papillary thyroid carcinoma and follicular adenoma. The 3 CDR on chromosome 3p may harbor tumor suppressor genes involved in the pathogenesis of follicular thyroid carcinoma and papillary thyroid carcinoma.
Hu
Meng-Jun
MJ
Department of Pathology, Zhuji People's Hospital, Zhuji 311800, China. sailing2121@163.com
Xu
Hang-Di
HD
Zhou
Ren
R
Li
Xiu-Fang
XF
Zhang
Hai-Yong
HY
D018263:Adenocarcinoma, Follicular / Q000235:genetics*; D000236:Adenoma / Q000235:genetics; D000328:Adult; D000368:Aged; D002291:Carcinoma, Papillary / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D005260:Female; D016147:Genes, Tumor Suppressor / Q000502:physiology; D006579:Heterozygote; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D013964:Thyroid Neoplasms / Q000235:genetics*; D055815:Young Adult