Micelles of lipid-oligonucleotide conjugates: implications for membrane anchoring and base pairing.
This report examines the organization properties of new fluorescent DNA-lipids, either alone in water or in interaction with 1-octyl-beta-d-glucopyranoside micelles or egg phosphatidylcholine vesicles. We first describe the design and the syntheses of the conjugates. Then, we use UV-Vis absorption, steady-state fluorescence emission, electron microscopy, and fluorescence correlation spectroscopy after two-photon excitation to show that these DNA-lipids form spherical micelles in aqueous solution and incorporate much better in micelles than in vesicles. We also investigate the significance of the lipophilic chains of these DNA-lipids on the melting behavior of the double-stranded hybrids: in water melting curves are broadened whereas in amphiphilic assemblies duplexes melt as the unconjugated controls. This work is expected to be useful for improving the rational design of antisense medicines.
Gosse
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C
Laboratoire de Physique Statistique CNRS UMR 8550, Ecole Normale Supérieure, 24 rue Lhomond, F-75231 Paris Cedex 05, France, Département de Chimie CNRS UMR 8640, Ecole Normale Supérieure, 24 rue Lhomond, F-75231 Paris Cedex 05, France, Laboratoire de Biophysique CNRS/MNHN UMR 5153, Muséum National d'Histoire Naturelle, 43 rue Cuvier, F-75231 Paris Cedex 05, France, and Laboratoire de Physico-Chimie CNRS UMR 168, Institut Curie, 26, rue d'Ulm, 75248 Paris Cedex 05, France.
Boutorine
A
A
Aujard
I
I
Chami
M
M
Kononov
A
A
Cogné-Laage
E
E
Allemand
J-F
JF
Li
J
J
Jullien
L
L