Hydroxyproline quantification for the estimation of collagen in tissue using multiple reaction monitoring mass spectrometry.
Collagens are highly abundant mammalian proteins that contain a high content of hydroxylated amino acids such as hydroxyproline. We have exploited the high hydroxyproline content of collagen and developed a method for hydroxyproline quantification as a measure of collagen content in muscle samples. The novel method utilizes a highly selective and sensitive method of multiple reaction monitoring (MRM) by mass spectrometry. The analytical method is simple, rapid (5min), convenient (no derivatization), precise (<17% RSD), accurate (90-108%), sensitive (4.88nmol/L) and linear (R(2)>0.999) over three orders of magnitude (5-5000nmol/L).
Colgrave
Michelle L
ML
Queensland Bioscience Precinct, St Lucia, QLD 4067, Australia. michelle.colgrave@csiro.au
Allingham
Peter G
PG
Jones
Alun
A
D000818:Animals; D002138:Calibration; D003094:Collagen / Q000032:analysis*; D006909:Hydroxyproline / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D009132:Muscles / Q000737:chemistry*; D011392:Proline / Q000032:analysis; D051381:Rats; D015203:Reproducibility of Results; D012756:Sheep
D003094:Collagen; D011392:Proline; D006909:Hydroxyproline