Comparative study for determination of Mycobacterium tuberculosis susceptibility to first- and second-line antituberculosis drugs by the Etest using 7H11, blood, and chocolate agar.
We investigated the performance of blood and chocolate agar as alternatives to Middlebrook 7H11 agar for testing the susceptibility of Mycobacterium tuberculosis to first-and second-line drugs by the Etest method. A total of 39 strains of M. tuberculosis including 22 multidrug-resistant M. tuberculosis strains and 17 susceptible strains were tested. In conclusion, our results showed that chocolate agar gave insufficient growth, needing up to 21 days of incubation, while results on blood agar were comparable to those on Middlebrook 7H11 agar and can be further explored as an alternative for Etest-based susceptibility testing of M. tuberculosis.
Coban
Ahmet Yilmaz
AY
Department of Microbiology and Clinical Microbiology, Ondokuz Mayis University Medical School, Samsun, Turkey. cobanay2003@yahoo.com.tr
Bilgin
Kemal
K
Uzun
Meltem
M
Akgunes
Alper
A
Yusof
Anne
A
Durupinar
Belma
B
D000362:Agar; D000995:Antitubercular Agents / Q000494:pharmacology*; D003470:Culture Media; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D014376:Tuberculosis / Q000382:microbiology
D000995:Antitubercular Agents; D003470:Culture Media; D000362:Agar