Proteomic analysis of the rat vestibular nucleus complex following unilateral labyrinthectomy.
CONCLUSIONS
The appearance of some candidate proteins may be essential for compensation as spatio-temporal expression patterns and this proteomic analysis may be a useful tool to understand the molecular basis of vestibular compensation.


OBJECTIVE
The purpose of this study was to identify the proteins expressed in vestibular nucleus complexes (VNCs) using proteomics following unilateral labyrinthectomy.


METHODS
Proteomic analysis was performed in VNCs of rats at 6 h, 72 h, and 7 days following unilateral labyrinthectomy in comparison to normal VNC. Twenty-seven interesting spots in both normal and study groups were selected for MALDI-TOF MS. Expression of some selected genes was confirmed by RT-PCR.


RESULTS
In all, 22 of 27 spots were matched after MALDI-TOF MS. Among these, 14 proteins were highly expressed in normal VNCs and 8 were newly expressed following labyrinthectomy. Among these 14 normal proteins, 9 were consistently expressed in both normal and labyrinthectomized VCNs, while 5 were down-regulated following labyrinthectomy. Eight were newly expressed following labyrinthectomy. Expression patterns of nine genes at RT-PCR were compatible with protein expression patterns.
Ban
Jae-Ho
JH
Department of Otolaryngology-Head and Neck Surgery and Department of Biomedical Sciences, Korea University College of Medicine, Seoul, 136-705, South Korea.
Chang
Jiwon
J
Im
Gi Jung
GJ
Jung
Hak Hyun
HH
D000818:Animals; D011184:Postoperative Period; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014726:Vestibular Nuclei / Q000378:metabolism*; D014722:Vestibule, Labyrinth / Q000601:surgery*