[Roles of regulated upon activation normal T cell expressed and secreted in pathogenesis of endometriosis].
OBJECTIVE
To explore the secretion of chemokine regulated upon activation normal T cell expressed and secreted (RANTES) influenced by the complex microenvironment in the peritoneal cavity of women with endometriosis and investigate chemotaxis of RANTES on the peritoneal monocytes.


METHODS
The contact and non-contact co-culture systems including three target cells of ectopic tissue were established. The three target cells were endometrial stromal cells (ESC), human peritoneal mesothelial cells (HPMC) and monocytes. After collection of the supernatant of co-culture systems, the levels of RANTES were detected by enzyme linked immunosorbent assay (ELISA). Migration of U937 cell, a monocyte line, was detected by chemotaxis assay.


RESULTS
ESC, HPMC, and U937 cultured alone secreted slight RANTES, (5.0 +/- 0.5), (4.0 +/- 0.3), and (254 +/- 40) ng/L. Compared with the culture of the target cell alone, the levels of RANTES in each co-culture system increased significantly, with the highest level in the contact culture system of E-H-U (2250 +/- 96) ng/L. RANTES secretion of non-contact co-culture of three cells were higher than contact co-culture of two cells (P < 0.01): U/E-H (912 +/- 93) vs E-H (50 +/- 40) ng/L, H/E-U (1201 +/- 93) vs E-U (243 +/- 192) ng/L,and E/H-U(1519 +/- 96) vs H-U (1251 +/- 73) ng/L. ESC, HPMC, and ESC-HPMC co-culture improved significantly migration of U937 cells [number of cell migration respectively (6.0 +/- 0.3), (6.2 +/- 0. 3) , (10.0 +/- 0.3) x 10(3), P < 0.01], which could be inhibited efficiently by anti-RANTES neutralizing antibody.


CONCLUSIONS
The target cells in the peritoneal cavity of patients with endometriosis promote the secretion of RANTES in autocrine and paracrine manners and migration of monocytes.
Yu
Jing
J
Laboratory of Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.
Wang
Yun
Y
Zhou
Wen-Hui
WH
Wang
Ling
L
Li
Da-Jin
DJ
D000328:Adult; D001202:Ascitic Fluid / Q000276:immunology*; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D054410:Chemokine CCL7 / Q000378:metabolism*; D018920:Coculture Techniques; D004715:Endometriosis / Q000209:etiology / Q000276:immunology* / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D020298:U937 Cells
D054410:Chemokine CCL7