Antiinflammatory effect of lactic acid bacteria: inhibition of cyclooxygenase-2 by suppressing nuclear factor-kappaB in Raw264.7 macrophage cells.
Lactobacillus casei 3260 (L. casei 3260) was evaluated in relation to the inflammatory response mediated by lipopolysaccharide (LPS)-induced nuclear factor-kappaB (NF-kappaB) and cyclooxygenase-2 (COX-2) expression in Raw264.7 macrophage cells. The treatment of Raw264.7 cells with L. casei 3260 significantly inhibited the secretion of tumor necrosis factor-alpha (TNF-alpha) and prostaglandins E2 (PGE2), followed by suppression of COX-2. To clarify the molecular mechanism, the inhibitory effect of L. casei 3260 on the NF-kappaB signaling pathway was examined based on the luciferase reporter activity. Although the treatment of Raw264.7 cells with L. casei 3260 did not affect the transcriptional activity of NF-kappaB, it did inhibit NF-kappaB activation, as determined by the cytosolic p65 release and degradation of I-kappaBalpha. Therefore, these findings suggest that the suppression of COX-2 through inhibiting the NF-kappaB activation by LPS may be associated with the antiinflammatory effects of L. casei 3260 on Raw264.7 cells.
Lee
Jeong Min
JM
Department of Medical Nutrition, Graduate School of East-West Medical Science and Research Institute of Clinical Nutrition,Kyung Hee University, Yongin 446-701, Korea.
Hwang
Kwon-Tack
KT
Jun
Woo Jin
WJ
Park
Chang-Soo
CS
Lee
Myung-Yul
MY
D000818:Animals; D002461:Cell Line, Transformed; D002470:Cell Survival; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000276:immunology*; D015232:Dinoprostone / Q000235:genetics / Q000276:immunology; D015536:Down-Regulation; D015870:Gene Expression; D007777:Lactobacillaceae / Q000235:genetics / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology*; D014158:Transcription, Genetic
D016328:NF-kappa B; D051546:Cyclooxygenase 2; D015232:Dinoprostone