Analysis of expression and comparative profile of normal placental tissue proteins and those in preeclampsia patients using proteomic approaches.
Preeclampsia (PE) is a complex and serious condition of pregnancy. Trophoblasts in human placenta can be separated and collected by laser capture micro-dissection (LCM). Protein in trophoblasts have been extracted and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), finally 962 unique proteins are identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). Comparison of differential expressed proteins in normal and those in PE are investigated. Two-dimensional electrophoresis (2DE) and MS were used to identify differential expressed proteins. 13 differential expressed proteins include signal transduction protein, molecular chaperone, cell skeleton proteins are identified, in which 3 proteins are down-regulated and 10 proteins are up-regulated. They might be correlated with the cause of PE.
Jin
Hong
H
Department of Chemistry, Fudan University, Shanghai 200433, China.
Ma
Kai-dong
KD
Hu
Rong
R
Chen
Yan
Y
Yang
Fengying
F
Yao
Jun
J
Li
Xiao-tian
XT
Yang
Peng-yuan
PY
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D004210:Dissection; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D020869:Gene Expression Profiling; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D007834:Lasers; D013058:Mass Spectrometry; D018832:Molecular Chaperones; D010920:Placenta / Q000166:cytology* / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D014327:Trophoblasts / Q000378:metabolism
D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D011257:Pregnancy Proteins