Naturally occurring feline leukemia virus subgroup A and B infections in urban domestic cats.
A nested-PCR (n-PCR) was used to detect feline leukemia virus (FeLV) proviral DNA in blood samples from 464 sick and 608 healthy domestic cats (Felis catus) selected by convenience, and a significantly high prevalence of FeLV infection was observed. n-PCR results revealed the presence of FeLV proviral DNA in 47.2 % of sick cats and 47.4 % of healthy cats. Phylogenetic analysis revealed that FeLV samples from healthy or sick cats were grouped into separate clades. We determined FeLV subgroups by an n-PCR based on the envelope (env) gene. The partial env gene of FeLV Minas Gerais (MG) samples were compared to various exogenous FeLV isolates and endogenous (enFeLV) provirus from the same region. FeLV-B MG samples were more similar to endogenous sequences and to natural FeLV-B isolates than to either FeLV-A or FeLV-C. The results revealed the circulation of FeLV-B in large populations of urban domestic cats in Brazil.
Coelho
Fabiana Magalhães
FM
Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Bomfim
Maria Rosa Quaresma
MRQ
Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
de Andrade Caxito
Fabíola
F
Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Ribeiro
Natália Almeida
NA
Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Luppi
Marcela Miranda
MM
Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Costa
Érica Azevedo
ÉA
Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Oliveira
Maria Emilia
ME
Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Da Fonseca
Flávio Guimarães
FG
Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Resende
Mauricio
M
Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP: 31270-901, Belo Horizonte, MG, Brazil.
D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015752:Genes, env; D006304:Health Status; D005256:Leukemia Virus, Feline / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016582:Leukemia, Feline / Q000821:virology*; D008019:Life Style; D008297:Male; D008969:Molecular Sequence Data; D011533:Proviruses / Q000302:isolation & purification; D012016:Reference Values; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014505:Urban Population; D014759:Viral Envelope Proteins / Q000737:chemistry
D004279:DNA, Viral; D014759:Viral Envelope Proteins