title: Drosophila hold'em is required for a subset of meiotic crossovers and interacts with the dna repair endonuclease complex subunits MEI-9 and ERCC1.
abstract: Three Drosophila proteins, ERCC1, MUS312, and MEI-9, function in a complex proposed to resolve double-Holliday-junction intermediates into crossovers during meiosis. We report here the characterization of hold'em (hdm), whose protein product belongs to a single-strand-DNA-binding superfamily of proteins. Mutations in hdm result in reduced meiotic crossover formation and sensitivity to the DNA-damaging agent methyl methanesulfonate. Furthermore, HDM physically interacts with both MEI-9 and ERCC1 in a yeast two-hybrid assay. We conclude that HDM, MEI-9, MUS312, and ERCC1 form a complex that resolves meiotic recombination intermediates into crossovers.
authors.affiliation: Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, New Jersey 08854-8020, USA.
authors.forename: Eric F
authors.initials: EF
authors.lastname: Joyce
authors.forename: S Nikhila
authors.initials: SN
authors.lastname: Tanneti
authors.forename: Kim S
authors.initials: KS
authors.lastname: McKim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003434:Crossing Over, Genetic / Q000187:drug effects; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D008540:Meiosis; D008741:Methyl Methanesulfonate / Q000633:toxicity; D008969:Molecular Sequence Data; D009153:Mutagens / Q000633:toxicity; D009154:Mutation / Q000235:genetics; D009630:Nondisjunction, Genetic / Q000187:drug effects; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D021122:Protein Subunits / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C538904:ERCC1 protein, Drosophila; C097527:MEI-9 protein, Drosophila; D009153:Mutagens; D009687:Nuclear Proteins; D021122:Protein Subunits; C538903:hdm protein, Drosophila; D008741:Methyl Methanesulfonate