title: JADAS: a customizable automated data acquisition system and its application to ice-embedded single particles.
abstract: The JEOL Automated Data Acquisition System (JADAS) is a software system built for the latest generation of the JEOL Transmission Electron Microscopes. It is designed to partially or fully automate image acquisition for ice-embedded single particles under low dose conditions. Its built-in flexibility permits users to customize the order of various imaging operations. In this paper, we describe how JADAS is used to accurately locate and image suitable specimen areas on a grid of ice-embedded particles. We also demonstrate the utility of JADAS by imaging the epsilon 15 bacteriophage with the JEM3200FSC electron cryo-microscope, showing that sufficient images can be collected in a single 8h session to yield a subnanometer resolution structure which agrees with the previously determined structure.
authors.affiliation: Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.forename: Natsuko
authors.initials: N
authors.lastname: Nakamura
authors.forename: Yuko
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Nathan
authors.initials: N
authors.lastname: Liang
authors.forename: Xiangan
authors.initials: X
authors.lastname: Liu
authors.forename: Joanita
authors.initials: J
authors.lastname: Jakana
authors.forename: Michael P
authors.initials: MP
authors.lastname: Marsh
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Booth
authors.forename: Takao
authors.initials: T
authors.lastname: Shinkawa
authors.forename: Munetaka
authors.initials: M
authors.lastname: Nakata
authors.forename: Wah
authors.initials: W
authors.lastname: Chiu
mesh_terms: D000465:Algorithms; D001331:Automation; D001435:Bacteriophages / Q000648:ultrastructure*; D020285:Cryoelectron Microscopy; D007053:Ice; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D012984:Software
chemical_list: D007053:Ice