title: Rapid diagnosis of thalassemias and other hemoglobinopathies by capillary electrophoresis system.
abstract: Basic diagnosis of hemoglobinopathies can be performed by analysis of erythrocyte indices as well as by the separation and quantification of the common hemoglobin (Hb) fractions Hb A(2), Hb S, Hb C, Hb D, Hb E, and Hb F. This study used an automatic capillary zone electrophoresis system to diagnose various types of hemoglobinopathies common in the Thai population. A total of 459 adults were recruited, which consisted of normal, various types of thalassemia carriers, and thalassemia patients with different genotypes. Hb types and quantification of all Hb components were determined by an automated capillary zone electrophoresis. The automatic capillary electrophoresis system can separate and quantitate Hbs A, F, E, A(2), Constant Spring (CS), H, and Bart's in a way that is comparable with other Hb analysis methods. Moreover, the Hb A(2) peak can be distinguished clearly from the Hb E peak in individuals who carry Hb E. The slightly increased levels of Hb A(2), 3.5% +/- 0.4%, which is shown in the carriers of Hb E, confirm that Hb E is the silent phenotype of beta(+)-thalassemia.
authors.affiliation: Thalassemia Research Center, Institute of Science and Technology for Research and Development, the Department of Biochemistry, the Department of Biochemistry, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Pranee
authors.initials: P
authors.lastname: Winichagoon
authors.forename: Saovaros
authors.initials: S
authors.lastname: Svasti
authors.forename: Thongperm
authors.initials: T
authors.lastname: Munkongdee
authors.forename: Wantana
authors.initials: W
authors.lastname: Chaiya
authors.forename: Piatip
authors.initials: P
authors.lastname: Boonmongkol
authors.forename: Nawarath
authors.initials: N
authors.lastname: Chantrakul
authors.forename: Suthat
authors.initials: S
authors.lastname: Fucharoen
mesh_terms: D000328:Adult; D019075:Electrophoresis, Capillary / Q000379:methods*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006579:Heterozygote; D006801:Humans; D010641:Phenotype; D015203:Reproducibility of Results; D013789:Thalassemia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics
chemical_list: D006455:Hemoglobins, Abnormal