In vitro adipose tissue engineering using an electrospun nanofibrous scaffold.
Electrospun 3-dimensional nanofibrous scaffolds share morphologic similarities to collagen fibrils, and promote favorable biologic responses of seeded cells. In this study, we have fabricated a 3-dimensional nanofibrous scaffold made of poly L-lactic acid, and examined its ability to support and maintain the adipogenic differentiation of human bone marrow-derived mesenchymal stem cells in vitro. After a 21-day incubation, oil red O staining of constructs treated with adipogenic supplements revealed positive adipose-like staining, compared with lack of staining in untreated cultures. Semi-quantitative RT-PCR analysis of human bone marrow-derived mesenchymal stem cells cultured in adipogenic medium revealed highly elevated levels of adipogenesis-associated genes (1797-fold for lipoprotein lipase, and 5.6-fold for peroxisome proliferator-activated receptor gamma). Immunofluorescence staining of cellular constructs in adipogenic culture media showed the presence of lipoprotein lipase vesicles, a characteristic feature of adipose tissue. These results suggest that the poly L-lactic acid-based nanofibrous scaffold is a promising candidate for adult stem cell-based engineering of adipose tissue.
Shanti
Rabie M
RM
Department of Health and Human Services, Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA. shanti.rabie@gmail.com
Janjanin
Sasa
S
Li
Wan-Ju
WJ
Nesti
Leon J
LJ
Mueller
Michael B
MB
Tzeng
Megan B
MB
Tuan
Rocky S
RS
D050156:Adipogenesis; D004867:Equipment Design; D005455:Fluorescent Antibody Technique; D005796:Genes; D019344:Lactic Acid; D059630:Mesenchymal Stem Cells / Q000502:physiology; D036103:Nanotechnology / Q000295:instrumentation*; D011091:Polyesters; D011108:Polymers; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D012313:RNA