A novel l-amino acid ligase from bacillus licheniformis.
l-Amino acid alpha-ligase (EC 6.3.2.28) catalyzed formation of alpha-peptide bond in unprotected l-amino acids in an ATP-dependent manner. BL00235 gene in Bacillus licheniformis NBRC12200 coded as a new l-amino acid ligase. BL00235 substrate specificity was strict; only methionine or leucine was acceptable as dipeptide N-terminal residues.
Kino
Kuniki
K
Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Noguchi
Atsushi
A
Nakazawa
Yuji
Y
Yagasaki
Makoto
M
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000737:chemistry*
D010453:Peptide Synthases