title: Isolation and characterization of pluripotent human spermatogonial stem cell-derived cells.
abstract: Several reports have documented the derivation of pluripotent cells (multipotent germline stem cells) from spermatogonial stem cells obtained from the adult mouse testis. These spermatogonia-derived stem cells express embryonic stem cell markers and differentiate to the three primary germ layers, as well as the germline. Data indicate that derivation may involve reprogramming of endogenous spermatogonia in culture. Here, we report the derivation of human multipotent germline stem cells (hMGSCs) from a testis biopsy. The cells express distinct markers of pluripotency, form embryoid bodies that contain derivatives of all three germ layers, maintain a normal XY karyotype, are hypomethylated at the H19 locus, and express high levels of telomerase. Teratoma assays indicate the presence of human cells 8 weeks post-transplantation but limited teratoma formation. Thus, these data suggest the potential to derive pluripotent cells from human testis biopsies but indicate a need for novel strategies to optimize hMGSC culture conditions and reprogramming.
authors.affiliation: Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Palo Alto, California 94304-5542, USA.
authors.forename: Nina
authors.initials: N
authors.lastname: Kossack
authors.forename: Juanito
authors.initials: J
authors.lastname: Meneses
authors.forename: Shai
authors.initials: S
authors.lastname: Shefi
authors.forename: Ha Nam
authors.initials: HN
authors.lastname: Nguyen
authors.forename: Shawn
authors.initials: S
authors.lastname: Chavez
authors.forename: Cory
authors.initials: C
authors.lastname: Nicholas
authors.forename: Joerg
authors.initials: J
authors.lastname: Gromoll
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Turek
authors.forename: Renee A
authors.initials: RA
authors.lastname: Reijo-Pera
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D002454:Cell Differentiation; D002460:Cell Line; D002469:Cell Separation; D048430:Cell Shape; D019175:DNA Methylation; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics; D039902:Multipotent Stem Cells / Q000166:cytology; D009474:Neurons / Q000166:cytology; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D032681:Spectral Karyotyping; D013093:Spermatogonia / Q000166:cytology* / Q000378:metabolism; D013447:Sulfites; D019098:Telomerase / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism
chemical_list: D015415:Biomarkers; D050814:Octamer Transcription Factor-3; D013447:Sulfites; D019098:Telomerase; C042345:hydrogen sulfite