title: Selective separation and co-culture of cells by photo-induced enhancement of cell adhesion (PIECA).
abstract: Taking advantage of the phenomenon that animal cells adhering to a culture substrate are temporarily immobilized by light irradiation, we established a technique to manipulate the cells adhering to a culture substrate under microscopic observation. Using this technique, we demonstrated a separation of cells adhering to a culture substrate and fabrication of an elaborately patterned co-culture system.
authors.affiliation: Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Edahiro
authors.forename: Kimio
authors.initials: K
authors.lastname: Sumaru
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ooshima
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kanamori
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D018920:Coculture Techniques; D006224:Cricetinae; D003412:Cricetulus; D008027:Light