title: Gene expression profiling in glioblastoma and immunohistochemical evaluation of IGFBP-2 and CDC20.
abstract: Thirty-nine glial tumours (28 glioblastomas (GB) and 11 low-grade gliomas) were investigated with DNA microarrays to reveal a possible specific gene expression profile. Unsupervised classification through hierarchical cluster analysis identified two groups of tumours, the first composed of low-grade gliomas and the second mainly composed of GB. Nine genes were identified as most informative: seven were over-expressed in low-grade gliomas and under-expressed in GB; on the contrary, two genes, insulin-like growth factor binding protein 2 (IGFBP-2) and cell division cycle 20 homologue (CDC20), were over-expressed in GB and under-expressed in low-grade tumours. This same genetic profile was confirmed by reverse transcriptase polymerase chain reaction. Immunohistochemistry for IGFBP-2 was positive in 88.8% of the cases of GB and in only one low-grade glioma, whilst CDC20 immunostained 74.1% of the cases of GB and none low-grade glioma. This was confirmed in an additional series of cases studied with immunohistochemistry only. In conclusion, over-expression of mRNA levels of IGFBP-2 and CDC20 is highly related to GB, IGFBP-2 and CDC-20 gene and protein expressions are strongly correlated, and IGFBP-2 and CDC20 immunopositivity can be useful for the identification of GB in small biopsies.
authors.affiliation: Section of Pathology, Bellaria Hospital, University of Bologna, Bologna, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Marucci
authors.forename: Luca
authors.initials: L
authors.lastname: Morandi
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Magrini
authors.forename: Anna
authors.initials: A
authors.lastname: Farnedi
authors.forename: Enrico
authors.initials: E
authors.lastname: Franceschi
authors.forename: Rossella
authors.initials: R
authors.lastname: Miglio
authors.forename: Daniela
authors.initials: D
authors.lastname: Calò
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pession
authors.forename: Maria P
authors.initials: MP
authors.lastname: Foschini
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Eusebi
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000235:genetics / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D018971:Insulin-Like Growth Factor Binding Protein 2; D012333:RNA, Messenger; C577488:CDC20 protein, human