Pseudouridines in rRNA helix 69 play a role in loop stacking interactions.
The (1)H NMR spectra of RNAs representing E. coli 23S rRNA helix 69 with [1,3-(15)N]pseudouridine modification at specific sites reveal unique roles for pseudouridine in stabilizing base-stacking interactions in the hairpin loop region.
Desaulniers
Jean-Paul
JP
Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA.
Chang
Yu-Cheng
YC
Aduri
Raviprasad
R
Abeysirigunawardena
Sanjaya C
SC
SantaLucia
John
J
Chow
Christine S
CS
D020029:Base Pairing; D001483:Base Sequence; D055029:Inverted Repeat Sequences / Q000235:genetics; D009690:Nucleic Acid Conformation; D011522:Protons; D011560:Pseudouridine / Q000737:chemistry* / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000737:chemistry* / Q000235:genetics
D011522:Protons; D012338:RNA, Ribosomal, 23S; D011560:Pseudouridine