Structural and immunological analysis of circumsporozoite protein peptides: a further step in the identification of potential components of a minimal subunit-based, chemically synthesised antimalarial vaccine.
The Plasmodium falciparum circumsporozoite protein is considered a major antimalarial-vaccine target due to its involvement in sporozoite invasion of mosquito's salivary glands and human hepatocytes. The 4383, 4388 and 4389 CSP-conserved high activity hepatocyte binding peptides and their modified analogues were synthesised and their immunogenicity was tested in Aotus monkeys. Peptide 4388 modified analogues induced higher and more permanent antibody titers against sporozoites in approximately 40% of immunised monkeys; whilst peptides 4383 and 4389 modified analogues elicited high, long-lasting antibody titers as well as short-lived antibodies. (1)H NMR studies showed that native peptides displayed random conformations, whereas most modified immunogenic HABPs contained type I, II and IV beta-turn structures. HLA-DRbeta1* molecule binding assays revealed that 4383 modified HABPs bound to HLA-DRbeta1*0701/HLA-DRbeta1*0401 molecules, whilst 4388 and 4389 modified HABPs bound to HLA-DRbeta1*0401/HLA-DRbeta1*0101, respectively. The results support these high-immunogenic CSP-derived modified peptides' inclusion in a multi-antigenic, multistage, minimal subunit-based synthetic antimalarial vaccine.
Bermúdez
Adriana
A
Fundación Instituto de Inmunología de Colombia, Bogotá, Colombia.
Vanegas
Magnolia
M
Patarroyo
Manuel Elkin
ME
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D001029:Aotus trivirgatus / Q000276:immunology; D015153:Blotting, Western; D002942:Circular Dichroism; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006684:HLA-DR Antigens / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D059811:HLA-DRB1 Chains; D007114:Immunization; D009682:Magnetic Resonance Spectroscopy; D017780:Malaria Vaccines / Q000138:chemical synthesis / Q000276:immunology*; D016778:Malaria, Falciparum / Q000276:immunology* / Q000517:prevention & control*; D052940:Merozoites / Q000276:immunology; D008958:Models, Molecular; D008968:Molecular Conformation; D015800:Protozoan Proteins / Q000276:immunology*; D022223:Vaccines, Subunit / Q000138:chemical synthesis / Q000276:immunology
D000953:Antigens, Protozoan; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D017780:Malaria Vaccines; D015800:Protozoan Proteins; D022223:Vaccines, Subunit; C041595:circumsporozoite protein, Protozoan