Disturbances in metabolic, transport and structural genes in experimental colonic inflammation in the rat: a longitudinal genomic analysis.
BACKGROUND
Trinitrobenzenesulphonic acid (TNBS) induced rat colitis is one of the most widely used models of inflammatory bowel disease (IBD), a condition whose aetiology and pathophysiology are incompletely understood. We have characterized this model at the genomic level using a longitudinal approach. Six control rats were compared with colitic animals at 2, 5, 7 and 14 days after TNBS administration (n = 3). The Affymetrix Rat Expression Array 230 2.0 system was used.


RESULTS
TNBS-induced colitis had a profound impact on the gene expression profile, which was maximal 5 and 7 days post-induction. Most genes were affected at more than one time point. They were related to a number of biological functions, not only inflammation/immunity but also transport, metabolism, signal transduction, tissue remodeling and angiogenesis. Gene changes generally correlated with the severity of colitis. The results were successfully validated in a subset of genes by real-time PCR.


CONCLUSIONS
The TNBS model of rat colitis has been described in detail at the transcriptome level. The changes observed correlate with pathophysiological disturbances such as tissue remodelling and alterations in ion transport, which are characteristic of both this model and IBD.
Martínez-Augustin
Olga
O
Department of Biochemistry and Molecular Biology II, CIBEREHD, School of Pharmacy, University of Granada, Granada, Spain. omartine@ugr.es
Merlos
Manel
M
Zarzuelo
Antonio
A
Suárez
María Dolores
MD
de Medina
Fermín Sánchez
FS
D000818:Animals; D016000:Cluster Analysis; D003092:Colitis / Q000139:chemically induced* / Q000235:genetics; D003106:Colon / Q000187:drug effects* / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D023281:Genomics; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity*
D014302:Trinitrobenzenesulfonic Acid