title: Transforming growth factor-alpha and nonsyndromic cleft lip with or without palate or cleft palate only in Kelantan, Malaysia.
abstract: OBJECTIVE
  To determine the frequency of the transforming growth factor-alpha (TGFalpha) Taq1 polymorphism in nonsyndromic cleft lip with or without cleft palate (CL+/-P) and cleft palate only (CP) in Kelantan, Malaysia.
  
  
  METHODS
  The study was conducted at the Combined Cleft Clinic and at the Human Genome Centre in Hospital Universiti Sains Malaysia in Kelantan, Malaysia.
  
  
  METHODS
  We examined the C2/Taq1 variant of the TGFalpha gene in 46 patients with nonsyndromic CL+/-P or CP only and in 33 controls. The TGFalpha genotype frequencies in patients were compared with those in controls using the chi-square or Fisher exact test. DNA samples were obtained from peripheral blood.
  
  
  RESULTS
  No association was found between TGFalphaTaq1 polymorphism and CL+/-P or CP in this case-control study. In addition, no homozygosity for the rare allele C2 was noted in CL+/-P, CP, or the controls.
  
  
  CONCLUSIONS
  No evidence of TGFalphaTaq1 polymorphism was observed in association with CL+/-P and CP in this study.
authors.affiliation: School of Dental Sciences, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, Malaysia. roselinda@kck.usm.my
authors.forename: Roselinda Abdul
authors.initials: RA
authors.lastname: Rahman
authors.forename: Azlina
authors.initials: A
authors.lastname: Ahmad
authors.forename: Zainal Ariff Abdul
authors.initials: ZA
authors.lastname: Rahman
authors.forename: Khairani Idah
authors.initials: KI
authors.lastname: Mokhtar
authors.forename: Nik Ahmad Shah Nik
authors.initials: NA
authors.lastname: Lah
authors.forename: Bin Alwi
authors.initials: BA
authors.lastname: Zilfalil
authors.forename: Ab Rani
authors.initials: AR
authors.lastname: Samsudin
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007223:Infant; D008296:Malaysia; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D019914:Taq Polymerase / Q000235:genetics; D016211:Transforming Growth Factor alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D016211:Transforming Growth Factor alpha; D019914:Taq Polymerase