title: Sequencing and analysis of approximately 40,000 soybean cDNA clones from a full-length-enriched cDNA library.
abstract: A large collection of full-length cDNAs is essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We obtained a total of 39,936 soybean cDNA clones (GMFL01 and GMFL02 clone sets) in a full-length-enriched cDNA library which was constructed from soybean plants that were grown under various developmental and environmental conditions. Sequencing from 5' and 3' ends of the clones generated 68 661 expressed sequence tags (ESTs). The EST sequences were clustered into 22,674 scaffolds involving 2580 full-length sequences. In addition, we sequenced 4712 full-length cDNAs. After removing overlaps, we obtained 6570 new full-length sequences of soybean cDNAs so far. Our data indicated that 87.7% of the soybean cDNA clones contain complete coding sequences in addition to 5'- and 3'-untranslated regions. All of the obtained data confirmed that our collection of soybean full-length cDNAs covers a wide variety of genes. Comparative analysis between the derived sequences from soybean and Arabidopsis, rice or other legumes data revealed that some specific genes were involved in our collection and a large part of them could be annotated to unknown functions. A large set of soybean full-length cDNA clones reported in this study will serve as a useful resource for gene discovery from soybean and will also aid a precise annotation of the soybean genome.
authors.affiliation: Gene Discovery Research Team, RIKEN Plant Science Center, Koyadai 3-1-1, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Taishi
authors.initials: T
authors.lastname: Umezawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Totoki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Toyoda
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ishiwata
authors.forename: Kenji
authors.initials: K
authors.lastname: Akiyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kurotani
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Yoshida
authors.forename: Keiichi
authors.initials: K
authors.lastname: Mochida
authors.forename: Mie
authors.initials: M
authors.lastname: Kasuga
authors.forename: Daisuke
authors.initials: D
authors.lastname: Todaka
authors.forename: Kyonoshin
authors.initials: K
authors.lastname: Maruyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nakashima
authors.forename: Akiko
authors.initials: A
authors.lastname: Enju
authors.forename: Saho
authors.initials: S
authors.lastname: Mizukado
authors.forename: Selina
authors.initials: S
authors.lastname: Ahmed
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yoshiwara
authors.forename: Kyuya
authors.initials: K
authors.lastname: Harada
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Tsubokura
authors.forename: Masaki
authors.initials: M
authors.lastname: Hayashi
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.forename: Toyoaki
authors.initials: T
authors.lastname: Anai
authors.forename: Masao
authors.initials: M
authors.lastname: Ishimoto
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Funatsuki
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Teraishi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Osaki
authors.forename: Takuro
authors.initials: T
authors.lastname: Shinano
authors.forename: Ryo
authors.initials: R
authors.lastname: Akashi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sakaki
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D020224:Expressed Sequence Tags / Q000737:chemistry; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018745:Genome, Plant; D008969:Molecular Sequence Data; D009348:Nematoda / Q000502:physiology; D010935:Plant Diseases / Q000469:parasitology; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000235:genetics / Q000254:growth & development / Q000469:parasitology / Q000502:physiology
chemical_list: D018076:DNA, Complementary