title: Crystallization and preliminary crystallographic studies of putative threonyl-tRNA synthetases from Aeropyrum pernix and Sulfolobus tokodaii.
abstract: Threonyl-tRNA synthetase (ThrRS) plays an essential role in protein synthesis by catalyzing the aminoacylation of tRNA(Thr) and editing misacylation. ThrRS generally contains an N-terminal editing domain, a catalytic domain and an anticodon-binding domain. The sequences of the editing domain in ThrRSs from archaea differ from those in bacteria and eukaryotes. Furthermore, several creanarchaea including Aeropyrum pernix K1 and Sulfolobus tokodaii strain 7 contain two genes encoding either the catalytic or the editing domain of ThrRS. To reveal the structural basis for this evolutionary divergence, the two types of ThrRS from the crenarchaea A. pernix and S. tokodaii have been overexpressed in Eschericha coli, purified and crystallized by the hanging-drop vapour-diffusion method. Diffraction data were collected and the structure of a selenomethionine-labelled A. pernix type-1 ThrRS crystal has been solved using the MAD method.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuda, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Shimizu
authors.forename: Ella Czarina Magat
authors.initials: EC
authors.lastname: Juan
authors.forename: Yu Ichiro
authors.initials: YI
authors.lastname: Miyashita
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Sato
authors.forename: Md Mominul
authors.initials: MM
authors.lastname: Hoque
authors.forename: Kaoru
authors.initials: K
authors.lastname: Suzuki
authors.forename: Masataka
authors.initials: M
authors.lastname: Yogiashi
authors.forename: Masaru
authors.initials: M
authors.lastname: Tsunoda
authors.forename: Anne Catherine
authors.initials: AC
authors.lastname: Dock-Bregeon
authors.forename: Dino
authors.initials: D
authors.lastname: Moras
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sekiguchi
authors.forename: Akio
authors.initials: A
authors.lastname: Takénaka
mesh_terms: D041522:Aeropyrum / Q000201:enzymology*; D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D008958:Models, Molecular; D011487:Protein Conformation; D013045:Species Specificity; D016944:Sulfolobus / Q000201:enzymology*; D013914:Threonine-tRNA Ligase / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D019843:Archaeal Proteins; D013914:Threonine-tRNA Ligase