title: 1,25(OH)2D3 regulates collagen quality in an osteoblastic cell culture system.
abstract: The active form of vitamin D, 1,25(OH)(2)D(3), has a broad range of effects on bone, however, its role in the quality of bone matrix is not well understood. In this study, using an osteoblastic cell (MC3T3-E1) culture system, the effects of 1,25(OH)(2)D(3) on collagen cross-linking and related enzymes, i.e., lysyl hydroxylases (LH1-3) and lysyl oxidases (LOX, LOXL1-4), were examined and compared to controls where cells were treated with cholecalciferol or ethanol. When compared to the controls, gene expressions of LH1, LH2b and LOXL2 were significantly upregulated by 1,25(OH)(2)D(3) up to 72h of culture. In addition, hydroxylysine (Hyl), Hyl aldehyde (Hyl(ald)), Hyl(ald)-derived cross-links and a total number of cross-links of collagen were significantly higher and the cross-link maturation was accelerated in the 1,25(OH)(2)D(3) treated group. These results demonstrate that 1,25(OH)(2)D(3) directly regulates collagen cross-linking in this culture system likely by upregulating gene expression of specific LH and LOX enzymes.
authors.affiliation: CB#7455, Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7455, USA; Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagaoka
authors.affiliation: CB#7455, Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7455, USA.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Mochida
authors.affiliation: CB#7455, Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7455, USA.
authors.forename: Phimon
authors.initials: P
authors.lastname: Atsawasuwan
authors.affiliation: CB#7455, Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7455, USA.
authors.forename: Masaru
authors.initials: M
authors.lastname: Kaku
authors.affiliation: Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan.
authors.forename: Toshirou
authors.initials: T
authors.lastname: Kondoh
authors.affiliation: CB#7455, Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7455, USA. Electronic address: Mitsuo_Yamauchi@dentistry.unc.edu.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Yamauchi
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D015415:Biomarkers; D002117:Calcitriol / Q000494:pharmacology / Q000502:physiology*; D002460:Cell Line; D002762:Cholecalciferol / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D010012:Osteogenesis; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D002762:Cholecalciferol; D000431:Ethanol; D003094:Collagen; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase; D008249:Protein-Lysine 6-Oxidase; D002117:Calcitriol; D008239:Lysine