Preparation of fluorescent magnetic nanodiamonds and cellular imaging.
Magnetic nanodiamonds were prepared via solid-state microwave arcing of a nanodiamond-ferrocene mixed powder in a focused microwave oven. High-resolution transmission electron microscope (HRTEM) images show that a magnetic nanodiamond is composed of iron nanoparticles encapsulated by graphene layers on the surface of nanodiamonds. Fluorescence property was introduced onto magnetic nanodiamonds by chemical modification of magnetic nanodiamonds via surface grafting of poly(acrylic acids) and fluorescein o-methacrylate. Fluorescent magnetic nanodiamonds are water soluble with a solubility of approximately 2.1 g/L. Cellular-imaging experiments show that fluorescent magnetic nanodiamonds could be ingested by HeLa cells readily in the absence of agonist (i.e., folate) moieties on the surface of nanodiamonds.
Chang
In Pin
IP
Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.
Hwang
Kuo Chu
KC
Chiang
Chi-Shiun
CS
D002470:Cell Survival / Q000187:drug effects; D018130:Diamond / Q000737:chemistry* / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D005456:Fluorescent Dyes; D018130:Diamond