MicroRNA expression profile of bronchioalveolar stem cells from mouse lung.
Increasing evidence has suggested that bronchioalveolar stem cell (BASC) is the progenitor cells of lung cancer stem cells. However, the mechanisms by which self-renewal of BSACs is controlled and how BASCs turn into cancer stem cells still remains to be unknown. In the present study, we successfully isolated bronchioalveolar stem cells (BASCs) from mouse lung using FACS. These BASCs were characterized by clonal growth, self-renewal and high capacity for differentiation, suggesting that these BASCs are indeed stem cells. We investigated the microRNA (miRNA) expression profile of these BASCs using miRNA array and quantitative RT-PCR. We discovered that BASCs possessed a unique miRNA profile, with altered expression of several microRNAs, such as miR-142-3p, miR-451, miR-106a, miR-142-5p, miR-15b, miR-20a, miR-106b, miR-25, miR-486, in BASCs compared to control cells. Our results suggest that microRNAs might play important roles in maintaining the self-renewal capacity of BASCs, and suggest the intriguing possibility that aberrant expression of microRNAs could involved in turning BASCs into lung cancer stem cells.
Qian
Shen
S
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
Ding
Jin-Yong
JY
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
Xie
Rongkai
R
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
An
Jiang-Hong
JH
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
Ao
Xu-Jun
XJ
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
Zhao
Zhen-Guo
ZG
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
Sun
Jian-Guo
JG
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
Duan
Yu-Zhong
YZ
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China.
Chen
Zheng-Tang
ZT
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China. Electronic address: zhengtangchen@yahoo.com.cn.
Zhu
Bo
B
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Xinquiao Street No. 2, Shapingba Di, Chongqing 400037, PR China. Electronic address: oncology_bozhu@yahoo.com.cn.
D000818:Animals; D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D020869:Gene Expression Profiling; D008175:Lung Neoplasms / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*
D035683:MicroRNAs