Molecular characterizations of avian polyomavirus isolated from budgerigar in China.
Budgerigar fledgling disease is an acute viral infectious disease caused by avian polyomavirus (APV). In this study, 34 liver tissue samples of young, dead budgerigar with typical symptoms were collected in 2004. All the samples had positive polymerase chain reaction (PCR) test based on the VP1 specific primers. VP1 genes of these samples were sequenced and had high similarities to each other (99%-100%). A strain (HBYM02) was isolated and sequenced. As shown in the phylogenetic tree, there are two branches. One branch was composed by strains isolated from Passeriformes, and the other was composed only by one strain isolated from Falconiformes. The genome similarities between our isolate and other reported isolates were very high (> 99%), and the evolution distances in the phylogenetic tree were very short (< 0.005), which suggests that APV in China has the same genotype as those in other regions. The results will be useful for the diagnoses of, and vaccine development for, APV.
Kou
Zheng
Z
Department of System Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Zhang
Zhong
Z
Chen
Shengliang
S
Fan
Zhaojun
Z
Tang
Shuang
S
Zhao
Lin
L
Li
Tianxian
T
D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D002681:China; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D046408:Melopsittacus / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011120:Polyomavirus / Q000235:genetics*; D017422:Sequence Analysis, DNA
D036022:Capsid Proteins; D017931:DNA Primers; C062481:VP1 protein, polyomavirus