Hyaluronan substratum holds mesenchymal stem cells in slow-cycling mode by prolonging G1 phase.
We examined, in vitro, whether hyaluronan induces slow cycling in placenta-derived mesenchymal stem cells (PDMSCs) by comparing cell growth on a hyaluronan-coated surface with cell growth on a tissue-culture polystyrene surface. The hyaluronan-coated surface significantly downregulated the proliferation of PDMSCs, more of which were maintained in the G(0)/G(1) phases than were cells on the tissue-culture polystyrene surface. Both PKH-26 labeling and BrdU incorporation assays showed that most PDMSCs grown on a hyaluronan-coated surface duplicated during cultivation indicating that the hyaluronan-coated surface did not inhibit PDMSCs from entering the cell cycle. Mitotic synchronization showed that the G(1)-phase transit was prolonged in PDMSCs growing on a hyaluronan-coated surface. Increases in p27(Kip1) and p130 were the crucial factors that allowed hyaluronan to lengthen the G(1) phase. Thus, hyaluronan might be a promising candidate for maintaining stem cells in slow-cycling mode by prolonging their G(1)-phase transit.
Liu
Chi-Mou
CM
Institute of Biotechnology and Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
Yu
Chen-Hsiang
CH
Chang
Chiung-Hsin
CH
Hsu
Chao-Chin
CC
Huang
Lynn L H
LL
D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects*; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D011247:Pregnancy; D013499:Surface Properties / Q000187:drug effects
D050760:Cyclin-Dependent Kinase Inhibitor p27; D006820:Hyaluronic Acid