[Role of serum from asphyxiated neonates in the inducement of human renal tubular cell adhesion to neutrophils].
OBJECTIVE
To study the role of serum from asphyxiated neonates in the inducement of human renal proximal tubular epithelial cells (HK-2) adhesion to neutrophils and possible mechanisms.


METHODS
HK-2 cells were cultured randomly with 20% serum from neonates (1, 3, and 7 days after asphyxia), pyrrolidine dithiocarbamate (PDTC) or placebo. The activity of myeloperoxidase (MPO), an indicator of adhesion ability of HK-2 cells to neutrophils in suspensions, was detected by the biochemistry assay. Intercellular adhesion molecule-1 (ICAM-1) and nuclear factor-kappaB (NF-kappaB) of HK-2 cells were examined with the immunohistochemical staining.


RESULTS
The expression of MPO in the post-asphyxial 1-day serum treatment group were significantly higher than that in the PDTC treatment and the control groups as well as the post-asphyxial 3 and 7-day serum treatment groups (P<0.01). The expression of ICAM-1 and NF-kappaB in the post-asphyxial 1-day serum treatment group was also significantly higher than that in the other groups (P<0.01).


CONCLUSIONS
Serum from asphyxiated neonates can induce HK-2 cell adhesion to neutrophils, possibly through activating NF-kappaB and increasing the synthesis and expression of ICAM-1 on the surface of renal tubular epithelial cells.
Fu
Hui
H
Department of Neonatology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, China.
Dong
Wen-Bin
WB
Long
Han-An
HA
Deng
Cun-Liang
CL
Wang
Ming-Yong
MY
Xu
Kai-Gui
KG
D001238:Asphyxia Neonatorum / Q000097:blood* / Q000150:complications; D002448:Cell Adhesion; D002478:Cells, Cultured; D006801:Humans; D007231:Infant, Newborn; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis; D007684:Kidney Tubules / Q000473:pathology*; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology*
D016328:NF-kappa B; D018799:Intercellular Adhesion Molecule-1