Headspace solid phase microextraction/gas chromatography-mass spectrometry combined to chemometric analysis for volatile organic compounds determination in canine hair: a new tool to detect dog contamination by visceral leishmaniasis.
A new analytical methodology using HS-SPME/GC-MS was optimized in order to attain maximum sensitivity, using multivariate strategies. The proposed method was employed to evaluate the VOC profile exhaled from canine hair samples collected from 8 healthy dogs and from 16 dogs infected by Leishmania infantum. 274 VOCs were detected, which could be identified as aldehydes, ketones and hydrocarbons. After application of the Soft Independent Modeling of Class Analogy (SIMCA) and Principal Component Analysis (PCA) healthy and infected dogs, with similar VOCs profiles, could be separately grouped, based on compounds such as 2-hexanone, benzaldehyde, and 2,4-nonadienal. The proposed method is non-invasive, painless, readily accepted by dog owners and could be useful to identify several biomarkers with applications in the diagnosis of diseases.
de Oliveira
Lidia S
LS
Instituto de Química, Universidade Federal da Bahia, Campus Universitário de Ondina, 40170-290 Salvador, BA, Brazil.
Rodrigues
Frederico de M
Fde M
de Oliveira
Fabio S
FS
Mesquita
Paulo R R
PR
Leal
Danielle C
DC
Alcântara
Adriano C
AC
Souza
Barbara M
BM
Franke
Carlos R
CR
Pereira
Pedro A de P
PA
de Andrade
Jailson B
JB
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000662:veterinary*; D016014:Linear Models; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D052617:Solid Phase Microextraction / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis*
D015415:Biomarkers; D055549:Volatile Organic Compounds