The p53 codon 72 single nucleotide polymorphism lacks a significant effect on implantation rate in fresh in vitro fertilization cycles: an analysis of 1,056 patients.
OBJECTIVE
To determine whether the p53 codon 72 single nucleotide polymorphism, a change of the amino acid arginine (Arg) to proline (Pro) resulting from a single nucleotide mutation of guanine (G) to cytosine (C), has a clinically significant effect on implantation rate in fresh IVF cycles.


METHODS
Prospective cohort analysis.


METHODS
University-affiliated private IVF center.


METHODS
One thousand fifty-six female patients undergoing fresh nondonor IVF cycles.


METHODS
Embryo implantation rate.


RESULTS
Of the 1,056 patients (2,600 total embryos transferred) undergoing their first IVF cycle, 289 had no implantation events and attempted a second cycle. Of the 289 patients in their second cycle, 72 had no implantation events and attempted a third cycle. The p53 codon 72 single nucleotide polymorphism frequencies in the first cycle (homozygous major allele Arg/Arg [G_G] = 45%, heterozygous allele Arg/Pro [G_C] = 44%, and homozygous minor allele Pro/Pro [C_C] = 11%) did not differ significantly across subsequent IVF cycles. There was no statistically significant difference in embryo implantation rate with respect to the single nucleotide polymorphism.


CONCLUSIONS
The p53 codon 72 single nucleotide polymorphism lacks a clinically significant effect on embryo implantation rate in patients undergoing fresh nondonor IVF cycles.
Patounakis
George
G
University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey.
Treff
Nathan
N
University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey; Reproductive Medicine Associates of New Jersey, Morristown, New Jersey.
Tao
Xin
X
Reproductive Medicine Associates of New Jersey, Morristown, New Jersey.
Lonczak
Agnieszka
A
Reproductive Medicine Associates of New Jersey, Morristown, New Jersey.
Scott
Richard T
RT
University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey; Reproductive Medicine Associates of New Jersey, Morristown, New Jersey.
Frattarelli
John L
JL
University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey; Reproductive Medicine Associates of New Jersey, Morristown, New Jersey. Electronic address: jfrattarelli@rmanj.com.
D003062:Codon / Q000235:genetics; D015331:Cohort Studies; D010064:Embryo Implantation / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005787:Gene Frequency; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007247:Infertility, Female / Q000175:diagnosis / Q000235:genetics / Q000628:therapy; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D011247:Pregnancy; D018873:Pregnancy Rate
D003062:Codon