Molecular cloning and functional analysis of minD gene from streptomyces lavendulae ATCC25233.
The minD gene encodes an inhibitor of cell division, which is involved in the regulation of the placement of the proper cell division site in rod-shaped bacteria. In the present study, we cloned the MinD homolog from the filamentous bacterium Streptomyces lavendulae ATCC25233. We showed that the protein exhibits ATPase activity and gives rise to a filamentous phenotype in Escherichia coli. However, the introduction of minD into Streptomyces lividans gave no apparent morphological alternation. We purified the Streptomyces minD gene product, expressed it in E. coli, and characterized it by enzyme kinetics. This is the first report characterizing the Streptomyces MinD homolog.
Nguyen
Tu Hoang Khue
TH
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Kumagai
Takanori
T
Matoba
Yasuyuki
Y
Suzaki
Toshinobu
T
Sugiyama
Masanori
M
D000251:Adenosine Triphosphatases / Q000737:chemistry*; D003001:Cloning, Molecular / Q000379:methods*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*
D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D000251:Adenosine Triphosphatases; C072069:MinD protein, E coli