Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment.
Rice straw was irradiated using an electron beam at currents and then hydrolyzed with cellulase and beta-glucosidase to produce glucose. The pretreatment by electron beam irradiation (EBI) was found to significantly increase the enzyme digestibility of rice straw. Specifically, when rice straw that was pretreated by EBI at 80 kGy at 0.12 mA and 1 MeV was hydrolyzed with 60 FPU of cellulase and 30 CBU of beta-glucosidase, the glucose yield after 132 h of hydrolysis was 52.1% of theoretical maximum. This value was significantly higher than the 22.6% that was obtained when untreated rice straw was used. In addition, SEM analysis of pretreated rice straw revealed that EBI caused apparent damage to the surface of the rice straw. Furthermore, EBI pretreatment was found to increase the crystalline portion of the rice straw. Finally, the crystallinity and enzyme digestibility were found to be strongly correlated between rice straw samples that were pretreated by EBI under different conditions.
Bak
Jin Seop
JS
Brain Korea 21 School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Ko
Ja Kyong
JK
Han
Young Hwan
YH
Lee
Byung Cheol
BC
Choi
In-Geol
IG
Kim
Kyoung Heon
KH
D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D003198:Computer Simulation; D004307:Dose-Response Relationship, Radiation; D004583:Electrons; D004789:Enzyme Activation / Q000528:radiation effects; D006868:Hydrolysis / Q000528:radiation effects; D008954:Models, Biological; D012275:Oryza / Q000737:chemistry* / Q000528:radiation effects*; D035261:Plant Components, Aerial / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage
D002241:Carbohydrates; D002482:Cellulose; D002480:Cellulase