title: Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase: a chemistry-biology interdisciplinary study of the protein's structure-function-reaction mechanism relationships.
abstract: The oxidosqualene cyclases (EC 5.4.99-) constitute a family of enzymes that catalyze diverse cyclization/rearrangement reactions of (3S)-2,3-oxidosqualene into a distinct array of sterols and triterpenes. The relationship between the cyclization mechanism and the enzymatic structure is extremely complex and compelling. This review covers the historical achievements of biomimetic studies and current progress in structural biology, molecular genetics, and bioinformatics studies to elucidate the mechanistic and structure-function relationships of the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase-catalyzed cyclization/rearrangement reaction.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, 75, Po-Ai Street, Hsin-Chu 300, Taiwan, ROC. tkwmll@mail.nctu.edu.tw
authors.forename: Tung-Kung
authors.initials: TK
authors.lastname: Wu
authors.forename: Cheng-Hsiang
authors.initials: CH
authors.lastname: Chang
authors.forename: Yuan-Ting
authors.initials: YT
authors.lastname: Liu
authors.forename: Tsai-Ting
authors.initials: TT
authors.lastname: Wang
mesh_terms: D002384:Catalysis; D003500:Cyclization; D019751:Intramolecular Transferases / Q000737:chemistry*; D008968:Molecular Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013185:Squalene / Q000031:analogs & derivatives / Q000737:chemistry; D013237:Stereoisomerism; D013261:Sterols / Q000138:chemical synthesis / Q000737:chemistry; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: C002821:2,3-oxidosqualene; D013261:Sterols; D014315:Triterpenes; D013185:Squalene; D019751:Intramolecular Transferases; C021924:lanosterol synthase