title: Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa.
abstract: Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. Fertility rate in buffalo following artificial insemination with frozen-thawed semen is reviewed. To better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. Moreover, the major factors that may influence the post-thaw viability and fertility of buffalo spermatozoa are examined in detail. In addition, suggestions for improvement in cryogenic procedures for buffalo spermatozoa are also given.
authors.affiliation: Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan. andrabi123@yahoo.com
authors.forename: S M H
authors.initials: SM
authors.lastname: Andrabi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D002020:Buffaloes; D002021:Buffers; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents; D005260:Female; D005298:Fertility; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D012621:Seasons; D012661:Semen / Q000737:chemistry; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D002021:Buffers; D003451:Cryoprotective Agents