title: Identification of an ISR-related metabolite produced by Pseudomonas chlororaphis O6 against the wildfire pathogen pseudomonas syringae pv.tabaci in tobacco.
abstract: Pseudomonas chlororaphis O6 exhibits induced systemic resistance (ISR) against P. syringae pv. tabaci in tobacco. To identify one of the ISR metabolites, O6 cultures were extracted with organic solvents, and the organic extracts were subjected to column chromatography followed by spectroscopy analyses. The ISR bioassay-guided fractionation was carried out for isolation of the metabolite. Highresolution mass spectrometric analysis of the metabolite found C(9)H(9)O(3)N with an exact mass of 179.0582. LC/MS analysis in positive mode showed an (M+H)(+) peak at m/zeta 180. Nuclear magnetic resonance ((1)H, (13)C) analyses identified all protons and carbons of the metabolite. Based on the spectroscopy data, the metabolite was identified 4-(aminocarbonyl) phenylacetate (4-ACPA). 4-ACPA applied at 68.0 mM exhibited ISR activity at a level similar 1.0 mM salicylic acid. This is the first report to identify an ISR metabolite produced by P. chlororaphis O6 against the wildfire pathogen P. syringae pv. tabaci in tobacco.
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Myung Ryeol
authors.initials: MR
authors.lastname: Park
authors.forename: Young Cheol
authors.initials: YC
authors.lastname: Kim
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Park
authors.forename: Song Hee
authors.initials: SH
authors.lastname: Han
authors.forename: Kil Yong
authors.initials: KY
authors.lastname: Kim
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Lee
authors.forename: In Seon
authors.initials: IS
authors.lastname: Kim
mesh_terms: D010648:Phenylacetates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology*; D011549:Pseudomonas / Q000737:chemistry* / Q000502:physiology*; D044224:Pseudomonas syringae / Q000187:drug effects*; D014026:Nicotiana / Q000382:microbiology*
chemical_list: D010648:Phenylacetates; C025136:phenylacetic acid