Linking SNPs to CAG repeat length in Huntington's disease patients.
Allele-specific silencing using small interfering RNAs targeting heterozygous single-nucleotide polymorphisms (SNPs) is a promising therapy for human trinucleotide repeat diseases such as Huntington's disease. Linking SNP identities to the two HTT alleles, normal and disease-causing, is a prerequisite for allele-specific RNA interference. Here we describe a method, SNP linkage by circularization (SLiC), to identify linkage between CAG repeat length and nucleotide identity of heterozygous SNPs using Huntington's disease patient peripheral blood samples.
Liu
Wanzhao
W
Department of Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA.
Kennington
Lori A
LA
Rosas
H Diana
HD
Hersch
Steven
S
Cha
Jang-Ho
JH
Zamore
Phillip D
PD
Aronin
Neil
N
D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018911:Trinucleotide Repeats / Q000235:genetics*