Isolation and structure determination of a proteasome inhibitory metabolite from a culture of Scytonema hofmanni.
Cyanobacteria, blue-green algae, are a rich source of bioactive secondary metabolites with many potential applications. The ubiquitin-proteasome proteolytic system plays an important role in selective protein degradation and regulates cellular events including apoptosis. Cancer cells are more sensitive to the proapoptotic effects of proteasome inhibition than normal cells. Thus, proteasome inhibitors can be potential anticancer agents. Cyanobacteria have been shown to be a rich source of highly effective inhibitors of proteases. A proteasome inhibitor was screened from an extract of the culture of Scytonema hofmanni on the basis of its inhibitory activity, which led to the isolation of nostodione A with an IC(50) value of 50 microM. Its structure was determined by spectroscopic methods such as 1H-NMR and ESI-MS spectral analyses.
Shim
Sang Hee
SH
School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea.
Chlipala
George
G
Orjala
Jimmy
J
D000458:Cyanobacteria / Q000737:chemistry / Q000378:metabolism*; D026121:Indole Alkaloids; D007211:Indoles / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D011480:Protease Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000378:metabolism; D061988:Proteasome Inhibitors
D026121:Indole Alkaloids; D007211:Indoles; D011480:Protease Inhibitors; D061988:Proteasome Inhibitors; C541082:nostodione A; D046988:Proteasome Endopeptidase Complex