Monitoring abortive initiation.
Abortive initiation, when first discovered, was an enigmatic phenomenon, but fully three decades hence, it has been shown to be an integral step in the transcript initiation process intimately tied to the promoter escape reaction undergone by RNA polymerase at the initiation-elongation transition. A detailed understanding of abortive initiation-promoter escape has brought within reach a full description of the transcription initiation mechanism. This enormous progress was the result of convergent biochemical, genetic, and biophysical investigations propelled by parallel advances in quantitation technology. This chapter discusses the knowledge gained through the biochemical approach and a high resolution method that yields quantitative and qualitative information regarding abortive initiation-promoter escape at a promoter.
Hsu
Lilian M
LM
Program in Biochemistry, Mount Holyoke College, 50 College Street, South Hadley, MA 01075, USA. lhsu@mtholyoke.edu
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D015967:Gene Expression Regulation, Viral; D007700:Kinetics; D008967:Molecular Biology / Q000379:methods; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000235:genetics*
D012321:DNA-Directed RNA Polymerases