title: The multienzyme architecture of eukaryotic fatty acid synthases.
abstract: Eukaryotic fatty acid synthases (FASs) are huge multifunctional enzymes that carry out all enzymatic steps essential for fatty acid biosynthesis. Recent crystallographic studies provide new insights into the architecture of the two distinct eukaryotic FAS systems, the 2.6 MDa heterododecameric fungal and the 540 kDa dimeric animal FAS. In this review, we compare the fundamentally different organization of these two megasynthases and discuss the structural principles of enzyme integration and substrate shuttling in FAS multienzymes.
authors.affiliation: Institute of Molecular Biology and Biophysics, ETH Zurich, 8093 Zurich, Switzerland.
authors.forename: Marc
authors.initials: M
authors.lastname: Leibundgut
authors.forename: Timm
authors.initials: T
authors.lastname: Maier
authors.forename: Simon
authors.initials: S
authors.lastname: Jenni
authors.forename: Nenad
authors.initials: N
authors.lastname: Ban
mesh_terms: D000818:Animals; D005057:Eukaryotic Cells / Q000201:enzymology*; D019143:Evolution, Molecular; D044943:Fatty Acid Desaturases / Q000737:chemistry; D064429:Fatty Acid Synthases / Q000737:chemistry* / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D006836:Hydro-Lyases / Q000737:chemistry; D015394:Molecular Structure; D010204:Pantetheine / Q000031:analogs & derivatives / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity; D014166:Transferases / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D010204:Pantetheine; D044943:Fatty Acid Desaturases; D014166:Transferases; D064429:Fatty Acid Synthases; D006836:Hydro-Lyases; C003129:4'-phosphopantetheine