Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies.
Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.
Morinaga
Osamu
O
Department of Pharmacognosy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan.
Uto
Takuhiro
T
Sakamoto
Seiichi
S
Tanaka
Hiroyuki
H
Shoyama
Yukihiro
Y
D000880:Anthraquinones / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal; D002366:Cassia / Q000737:chemistry*; D002400:Cathartics / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015203:Reproducibility of Results; D012250:Rheum / Q000737:chemistry*; D012676:Senna Extract / Q000737:chemistry; D029846:Senna Plant / Q000737:chemistry*; D000081226:Sennosides
D000880:Anthraquinones; D000911:Antibodies, Monoclonal; D002400:Cathartics; D000081226:Sennosides; D012676:Senna Extract