Molecular expression of Gastrokine 1 in normal mucosa and in Helicobacter pylori-related preneoplastic and neoplastic gastric lesions.
We have analyzed the expression of Gastrokine 1 (GKN1), one of the most abundant protein of gastric mucosa, in Helicobacter pylori-related preneoplastic and neoplastic gastric lesions. The GKN1 expression was downregulated in 36 H. pylori-positive patients with respect to 29 H. pylori-negative subjects as evaluated by Western blot, reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry, showing a progressive decrease from chronic gastritis to atrophy and intestinal metaplasia. Interestingly, in gastric cancer (GC) patients, GKN1 was undetectable in tissue tumoral areas but was instead expressed in the corresponding tissue non-tumoral areas. In conclusion our data suggests that GKN1 expression is related to the inflammatory damage of gastric mucosa and could be the related to the gastric cellular phenotype.
Nardone
Gerardo
G
Department of Clinical and Experimental Medicine, Gastroenterology Unit, University of Naples Federico II, Naples, Italy. nardone@unina.it
Martin
Giovanni
G
Rocco
Alba
A
Rippa
Emilia
E
La Monica
Giovanna
G
Caruso
Flora
F
Arcari
Paolo
P
D000328:Adult; D001284:Atrophy; D017931:DNA Primers; D004415:Dyspepsia / Q000235:genetics / Q000473:pathology; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology* / Q000502:physiology*; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008679:Metaplasia / Q000235:genetics / Q000473:pathology; D008875:Middle Aged; D036361:Peptide Hormones / Q000235:genetics*; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
D017931:DNA Primers; C477000:GKN1 protein, human; D036361:Peptide Hormones