NADPH oxidase-mediated generation of reactive oxygen species: A new mechanism for X-ray-induced HeLa cell death.
Oxidative damage is an important mechanism in X-ray-induced cell death. Radiolysis of water molecules is a source of reactive oxygen species (ROS) that contribute to X-ray-induced cell death. In this study, we showed by ROS detection and a cell survival assay that NADPH oxidase has a very important role in X-ray-induced cell death. Under X-ray irradiation, the upregulation of the expression of NADPH oxidase membrane subunit gp91(phox) was dose-dependent. Meanwhile, the cytoplasmic subunit p47(phox) was translocated to the cell membrane and localized with p22(phox) and gp91(phox) to form reactive NADPH oxidase. Our data suggest, for the first time, that NADPH oxidase-mediated generation of ROS is an important contributor to X-ray-induced cell death. This suggests a new target for combined gene transfer and radiotherapy.
Liu
Qing
Q
Department of Radiobiology, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou 730000, China.
He
Xiaoqing
X
Liu
Yongsheng
Y
Du
Bingbing
B
Wang
Xiaoyan
X
Zhang
Weisheng
W
Jia
Pengfei
P
Dong
Jingmei
J
Ma
Jianxiu
J
Wang
Xiaohu
X
Li
Sha
S
Zhang
Hong
H
D017209:Apoptosis; D015316:Genetic Therapy; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000532:radiotherapy / Q000628:therapy; D011836:Radiation Tolerance / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014965:X-Rays
D008562:Membrane Glycoproteins; D017382:Reactive Oxygen Species; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; C086986:CYBA protein, human; C060505:neutrophil cytosolic factor 1