title: Stimulation of endometrium embryo transfer can improve implantation and pregnancy rates for patients undergoing assisted reproductive technology for the first time with a high-grade blastocyst.
abstract: OBJECTIVE
  To evaluate whether stimulation of endometrium embryo transfer (SEET) can improve implantation rate and pregnancy rate (PR) for patients undergoing assisted reproductive technology (ART) for the first time by injecting embryo culture supernatant into the uterus before blastocyst transfer (BT).
  
  
  METHODS
  Randomized, controlled trial.
  
  
  METHODS
  Private in vitro fertilization clinic.
  
  
  METHODS
  Forty-eight women in the BT group, 48 women in the stimulation group who had culture medium injected into the uterus before BT, and 48 women in the SEET group.
  
  
  METHODS
  Injection of embryo culture supernatant and injection of culture medium.
  
  
  METHODS
  Implantation rates and PRs.
  
  
  RESULTS
  Odds ratios of successful implantation rate for stimulation and SEET in patients with high-grade blastocysts, having BT as reference, were 2.58 and 6.46 without adjustment, and 5.91 and 9.20 after adjusting for basal FSH levels and period of infertility. Odds ratios of clinical pregnancies were 2.47 and 4.32 without adjustment, and 4.46 and 5.10 with adjustment, respectively. In groups with low-grade blastocysts, such tendencies were not observed.
  
  
  CONCLUSIONS
  The SEET may be an effective method for increasing implantation rate and PR for first-time ART patients who have a high-grade blastocyst.
authors.affiliation: Hanabusa Women's Clinic, Kobe, Japan. Electronic address: gotoms@tcn.zaq.ne.jp.
authors.forename: Sakae
authors.initials: S
authors.lastname: Goto
authors.affiliation: Department of Health Science, Shiga University of Medical Science, Otsu, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kadowaki
authors.affiliation: Hanabusa Women's Clinic, Kobe, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hashimoto
authors.affiliation: Hanabusa Women's Clinic, Kobe, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Kokeguchi
authors.affiliation: Hanabusa Women's Clinic, Kobe, Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Shiotani
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000166:cytology; D003470:Culture Media / Q000494:pharmacology*; D046149:Embryo Culture Techniques; D010064:Embryo Implantation / Q000187:drug effects / Q000502:physiology; D004624:Embryo Transfer / Q000379:methods*; D004717:Endometrium / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000503:physiopathology / Q000628:therapy; D011247:Pregnancy; D018873:Pregnancy Rate; D011786:Quality Control; D027724:Reproductive Techniques, Assisted; D013268:Stimulation, Chemical; D016896:Treatment Outcome
chemical_list: D003470:Culture Media