Efficient bioethanol production from xylose by recombinant saccharomyces cerevisiae requires high activity of xylose reductase and moderate xylulokinase activity.
We varied the promoter strength of xylose reductase (XR) gene and the copy number of xylulokinase (XK) gene to determine how XR and XK activities affect the xylose-fermenting abilities of recombinant Saccharomyces cerevisiae expressing xylitol dehydrogenase (XDH). The most enhanced ethanol yield and lowered xylitol yield occurred in strain I-PGK/AUR, which has high activity of both XR and XDH and moderate XK activity.
Matsushika
Akinori
A
Biomass Technology Research Center (BTRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan.
Sawayama
Shigeki
S
D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism*; D001674:Bioelectric Energy Sources; D004789:Enzyme Activation; D000431:Ethanol / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
D011994:Recombinant Proteins; D000431:Ethanol; D014994:Xylose; D000449:Aldehyde Reductase; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase