[Expression of calbindin-D28k in genetic hypercalciuric stone-forming rats kidney and its role in pathogenesis of idiopathic hypercalciuria].
OBJECTIVE
To study the expression level of calbindin-D28k, a kind of calcium binding protein, in the kidneys of genetic hypercalciuric stone-forming (GHS) rats and to investigate its role in idiopathic hypercalciuria (IH).


METHODS
Kidneys were taken out from 16 GHS rats and 6 normal control (NC) rats. Western blotting and real time quantitative PCR were used to detect the protein and mRNA expression levels of calbindin-D28k respectively.


RESULTS
Western blotting showed that the A value of calbindin-D28k of the GHS rats was 0.49 +/- 0.02, significantly higher than that of the NC rats (0.20 +/- 0.01, P < 0.05). The 2(-(delta delta CT)) value of mRNA of calbindin-D28k of the GHS rats was 1.21, remarkably higher than that of the NC rats [with the of 2(-(delta delta CT)) value of 1.0]. There was not significant difference in the delta CT value between the two groups (P > 0.05).


CONCLUSIONS
The up-regulation of calbindin-D28k in the GHS rats is possibly caused by hyperexpression of VDR and hypercalcinuria, and plays an important role in urine calcium reabsorption; however, it is not the key protein that results in IH.
Wang
Shao-gang
SG
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. sgwangtjm@163.com
Hu
Dong-liang
DL
Xi
Qi-lin
QL
Bai
Jian
J
Su
Shi-qiang
SQ
Yu
Xiao
X
Liu
Ji-hong
JH
Ye
Zhang-qun
ZQ
D000818:Animals; D015153:Blotting, Western; D064092:Calbindin 1; D064026:Calbindins; D005260:Female; D015870:Gene Expression; D053565:Hypercalciuria / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis / Q000235:genetics*; D014545:Urinary Calculi / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G