Transgenic B7-H3 therapy induces tumor-specific immune response in human oral squamous cell cancer: an in vitro study.
OBJECTIVE
Tumors may present antigens to T cells but lack costimulatory signals which are necessary to initialize an effective immunologic response. This study aimed to develop a tumor cell-based cancer vaccine by genetically modifying oral squamous cell cancer (OSCC) cell line Tca8113 with human B7-H3 immunoglobulin, and to evaluate its efficacy in enhancing the tumor-specific immune response.


METHODS
Human B7-H3 gene was extracted from isolated T lymphocytes of healthy volunteers. Tumor cell vaccine TCV-hB7-H3 and mock control were prepared by transfecting Tca8113 cells with B7-H3 or mock vector. After being stimulated with TCV-hB7-H3 or mock control, the proliferation, IFN-mu expression, and cytotoxicity of the T cells were assessed.


RESULTS
The Tca8113 cells transfected with human B7-H3 significantly enhanced the proliferation, IFN-mu expression, and cytotoxicity of the T cells.


CONCLUSIONS
Genetically modified OSCC cells encoding B7-H3 enhance the induction of tumor specific immune response.
Yang
Hong Y
HY
Department of Oral and Maxillofacial Surgery, Shenzhen Hospital, Peking University, Shenzhen, China. hyyang192@hotmail.com
Chu
Mei
M
Zheng
Li W
LW
Zwahlen
Roger A
RA
Luo
Juan
J
Zou
Duo H
DH
Sun
Shun T
ST
D015703:Antigens, CD / Q000235:genetics / Q000627:therapeutic use*; D060887:B7 Antigens; D019496:Cancer Vaccines / Q000235:genetics / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007372:Interferons / Q000096:biosynthesis; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000276:immunology; D011971:Receptors, Immunologic / Q000235:genetics / Q000627:therapeutic use*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism; D019076:Transgenes
D015703:Antigens, CD; D060887:B7 Antigens; C423237:CD276 protein, human; D019496:Cancer Vaccines; D011971:Receptors, Immunologic; D007372:Interferons