title: Biphasic effect of insulin on beta cell apoptosis depending on glucose deprivation.
abstract: Insulin resistant states are associated with an increase in the beta cell mass and also high levels of circulating insulin. Ultimately the beta cells undergo a failure that leads to diabetes. At this stage, a question arises if those persistent high levels of circulating insulin may contribute to beta cell damage. To address this important issue, we submitted beta cells to a prolonged effect of increasing concentrations of insulin. We observed that a prolonged effect of high levels of insulin on the presence of serum (15-24h) in glucose-deprived beta cells induced apoptosis. This apoptotic effect was both dose- and cycloheximide-dependent.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid 28040, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Guillen
authors.forename: A
authors.initials: A
authors.lastname: Bartolomé
authors.forename: C
authors.initials: C
authors.lastname: Nevado
authors.forename: M
authors.initials: M
authors.lastname: Benito
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology
chemical_list: D007328:Insulin; D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide; D053148:Caspase 3; D005947:Glucose