Phototoxicity of phenylenediamine hair dye chemicals in Salmonella typhimurium TA102 and human skin keratinocytes.
Phenylenediamines (PD) are dye precursors used to manufacture hair dyes. The three PDs, 1,2-,1,3-, and 1,4-PD and three chlorinated PDs, 4-chloro-1,2-PD, 4-chloro-1,3-PD, and 4,5-dichloro-1,2-PD were studied for their mutagenic effect in Salmonella typhimurium TA 102, cytotoxicity in human skin keratinocyte cells, and for DNA cleavage. The results show that all six compounds are not toxic/mutagenic in TA 102 bacteria or skin cells, and do not cause DNA cleavage in PhiX 174 phage DNA. If the same tests are carried out by exposing them to light irradiation concurrently, all three chlorinated PDs cause mutation in TA 102 bacteria and single strand cleavage in PhiX174 phage DNA. This indicates that chlorination of the PDs makes these compounds more photochemically active and produces reactive species that cause DNA damage and mutation. For the photocytotoxicity test in skin cells, it appears there is no such structure-activity relationship. Two chlorinated PDs and two non-chlorinated PDs are cytotoxic at a fairly high concentration (1000microM) upon exposure to light irradiation.
Mosley-Foreman
Charity
C
Jackson State University, Jackson, MS 39217, USA.
Choi
Jaehwa
J
Wang
Shuguang
S
Yu
Hongtao
H
D010584:Bacteriophage phi X 174 / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D053904:DNA Breaks, Single-Stranded / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage / Q000187:drug effects; D017484:Dermatitis, Phototoxic; D006202:Hair Dyes / Q000633:toxicity*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D008027:Light; D009152:Mutagenicity Tests; D009153:Mutagens; D010655:Phenylenediamines / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics*; D012867:Skin / Q000187:drug effects / Q000528:radiation effects; D013329:Structure-Activity Relationship
D006202:Hair Dyes; D009153:Mutagens; D010655:Phenylenediamines