title: LC-MS/MS method for determination of hederacolchiside E, a neuroactive saponin from Pulsatilla koreana extract in rat plasma for pharmacokinetic study.
abstract: A simple, rapid, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was applied to pharmacokinetic study of a neuroactive oleanolic-glycoside saponin, hederacolchiside E from SK-PC-B70M, a standardized extract of Pulsatilla koreana in rat. Rat plasma samples were pretreated by protein precipitation with acetonitrile, eluted from C(18) column, and analyzed using electrospray ionization (ESI)-MS/MS in negative ion mode. Digoxin was used as an internal standard. The standard curves were linear (r>0.997) over the concentration ranges of 2-500 ng/mL. The intra- and inter-day precisions were measured to be below 9% and accuracy between 90 and 111% for all quality control samples at 2, 20, 100, and 500 ng/mL (n=5). The lower limits of quantification (LLOQ) for hederacolchiside E was 2 ng/mL and the limit of detection (LOD) 0.5 ng/mL using 20 microL of plasma sample. Subsequently, hederacolchiside E was determined in rat plasma samples after oral administration of SK-PC-B70M. The mean maximum plasma concentrations of hederacolchiside E were 0.07, 0.13, and 0.36 microg/mL and the mean areas under the plasma concentration versus time curve 0.56, 1.27, and 6.46 microg h/mL at doses of 100, 200, and 400 mg/kg, respectively, which indicated non-linear pharmacokinetic pattern. In conclusion, this method was successfully applied to the pharmacokinetic study of hederacolchiside E after an oral administration of SK-PC-B70M to rats.
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, PO Box 131, Chungryang, Seoul, Republic of Korea. behappy@kist.re.kr
authors.forename: Hye Hyun
authors.initials: HH
authors.lastname: Yoo
authors.forename: Sang Kyu
authors.initials: SK
authors.lastname: Lee
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Lim
authors.forename: Yoon
authors.initials: Y
authors.lastname: Kim
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kang
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Kim
authors.forename: Yang Hae
authors.initials: YH
authors.lastname: Park
authors.forename: Guang-Jin
authors.initials: GJ
authors.lastname: Im
authors.forename: Bong Yong
authors.initials: BY
authors.lastname: Lee
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D006207:Half-Life; D008297:Male; D015394:Molecular Structure; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000592:standards; D031946:Pulsatilla / Q000737:chemistry*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012503:Saponins / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000592:standards; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C527801:SK-PC-B70M; D012503:Saponins; C523728:hederacolchiside E