title: Transgenic B7-H3 therapy induces tumor-specific immune response in human oral squamous cell cancer: an in vitro study.
abstract: OBJECTIVE
  Tumors may present antigens to T cells but lack costimulatory signals which are necessary to initialize an effective immunologic response. This study aimed to develop a tumor cell-based cancer vaccine by genetically modifying oral squamous cell cancer (OSCC) cell line Tca8113 with human B7-H3 immunoglobulin, and to evaluate its efficacy in enhancing the tumor-specific immune response.
  
  
  METHODS
  Human B7-H3 gene was extracted from isolated T lymphocytes of healthy volunteers. Tumor cell vaccine TCV-hB7-H3 and mock control were prepared by transfecting Tca8113 cells with B7-H3 or mock vector. After being stimulated with TCV-hB7-H3 or mock control, the proliferation, IFN-mu expression, and cytotoxicity of the T cells were assessed.
  
  
  RESULTS
  The Tca8113 cells transfected with human B7-H3 significantly enhanced the proliferation, IFN-mu expression, and cytotoxicity of the T cells.
  
  
  CONCLUSIONS
  Genetically modified OSCC cells encoding B7-H3 enhance the induction of tumor specific immune response.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Shenzhen Hospital, Peking University, Shenzhen, China. hyyang192@hotmail.com
authors.forename: Hong Y
authors.initials: HY
authors.lastname: Yang
authors.forename: Mei
authors.initials: M
authors.lastname: Chu
authors.forename: Li W
authors.initials: LW
authors.lastname: Zheng
authors.forename: Roger A
authors.initials: RA
authors.lastname: Zwahlen
authors.forename: Juan
authors.initials: J
authors.lastname: Luo
authors.forename: Duo H
authors.initials: DH
authors.lastname: Zou
authors.forename: Shun T
authors.initials: ST
authors.lastname: Sun
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000627:therapeutic use*; D060887:B7 Antigens; D019496:Cancer Vaccines / Q000235:genetics / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007372:Interferons / Q000096:biosynthesis; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000276:immunology; D011971:Receptors, Immunologic / Q000235:genetics / Q000627:therapeutic use*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism; D019076:Transgenes
chemical_list: D015703:Antigens, CD; D060887:B7 Antigens; C423237:CD276 protein, human; D019496:Cancer Vaccines; D011971:Receptors, Immunologic; D007372:Interferons