title: Dimethyl sulfoxide perfusion in caprine ovarian tissue and its relationship with follicular viability after cryopreservation.
abstract: Ovarian cortical fragments (3 x 3 x 1 mm) were exposed to dimethyl sulfoxide (DMSO) in different concentrations for further analysis of cryoprotectant perfusion by applying high-performance liquid chromatography (HPLC) and conventional cryopreservation. This simple perfusion test can predict the efficiency of the cryopreservation procedure.
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles, University of Ceará, Fortaleza, Brazil.
authors.forename: Valesca B
authors.initials: VB
authors.lastname: Luz
authors.forename: Regiane R
authors.initials: RR
authors.lastname: Santos
authors.forename: Leonardo C
authors.initials: LC
authors.lastname: Pinto
authors.forename: Alison A X
authors.initials: AA
authors.lastname: Soares
authors.forename: Juliana J H
authors.initials: JJ
authors.lastname: Celestino
authors.forename: Jair
authors.initials: J
authors.lastname: Mafezoli
authors.forename: Cláudio C
authors.initials: CC
authors.lastname: Campello
authors.forename: José R
authors.initials: JR
authors.lastname: Figueiredo
authors.forename: Ana P R
authors.initials: AP
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006041:Goats; D023421:Models, Animal; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D010053:Ovary / Q000187:drug effects* / Q000502:physiology; D010477:Perfusion / Q000379:methods; D014022:Tissue Survival / Q000187:drug effects* / Q000502:physiology
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide