[Establishment of the method to induce and measure human IL-2 in vitro].
This study was aimed to establish the quantitative analysis of hIL-2 in culture supernatant by multifunctional Luminex 100. The lymphocytes were separated from ACD-anticoagulated peripheral blood by density gradient method. The lymphocytes were stimulated with PHA for 48 hours, and frozen at -20 degrees C The relative fluorescence units of standard preparations and samples were detected by multifunctional Luminex 100, and the sample concentrations were calculated by standard curve. The results indicated that the regression equation of standard preparation is Lg (RFU) = 1.547 + 0.867 LgC. ANOVA F = 301.7427, p < 0.05 (nu = 6). The analysis of variance showed F = 301.7427, p < 0.05 (nu = 6). The test of regression coefficient showed t = 17.3707 (nu = 6), p < 0.05. It is concluded that method for induction and measurement of human IL-2 in vitro is established. The standard curve established by this way is statistically significant. There is linear relationship between the concentration of hIL-2 and fluorescence intensity.
Li
Zhen
Z
Shenzhen Blood Center, Shenzhen, 518035, Guangdong Province, China. shallylizhen@yahoo.com.cn
Zhang
Wen-Lin
WL
Tang
Si
S
Cheng
Xi
X
Cheng
Liang-Hong
LH
Zhang
Yin-Ze
YZ
D002469:Cell Separation / Q000379:methods; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis*; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology
C508594:IL2 protein, human; D007376:Interleukin-2; D010835:Phytohemagglutinins