Identification of two differentially expressed forms of progranulin mRNA in the teleost fish Paralichthys olivaceus.
Two forms of progranulin mRNA were isolated from kidney and spleen cDNA libraries of the olive flounder, Paralichthys olivaceus. The 3'-untranslated regions (3'-UTRs) of these flounder progranulin (f-pgrn) mRNAs differed in a 20-nucleotide sequence element (5'-AACTGATTACGTTCAACAAC-3') that was present in one mRNA (designated f-pgrn type II) and not in the other (designated f-pgrn type I). Both mRNA sequences contained an open reading frame encoding a 289-amino-acid polypeptide of approximately 33 kDa. Southern blot analysis of the P. olivaceus flounder genome using an f-pgrn cDNA probe and a PCR-based approach identified a single copy of f-pgrn corresponding to the type II mRNA. The expression profiles of the two types of f-pgrn mRNA differed from each other and were tissue- and condition-dependent. The type II mRNA was detected abundantly in studied tissues (gill, kidney, spleen, and intestine) of non-stimulated healthy flounders. The type I mRNA was rarely expressed in any tissues of healthy flounders, but it was continuously increased in the examined tissues of flounders after the intraperitoneal injection of lipopolysaccharide. On the other hand, the expression of type II mRNA was decreased in inverse proportion to the type I mRNA in the LSP-stimulated flounders. These results suggest that type I and type II f-pgrn mRNA are translated into different proteins with different activities in the immune system of flounder.
Nam
Bo-Hye
BH
Biotechnology Research Institute, National Fisheries Research and Development Institute, Gijang-gun, Busan, Republic of Korea. bhnam@hanmail.net
Park
Eun-Mi
EM
Kim
Young-Ok
YO
Kong
Hee Jeong
HJ
Kim
Woo-Jin
WJ
Kang
Jung-Ha
JH
Ji
Young-Ju
YJ
Lee
Sang-Jun
SJ
Choi
Tae-Jin
TJ
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005432:Flounder / Q000235:genetics* / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects; D023061:Gene Order; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000276:immunology; D016415:Sequence Alignment
D000276:Adjuvants, Immunologic; D036341:Intercellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; D012333:RNA, Messenger