title: Various oils and detergents enhance the microbial production of farnesol and related prenyl alcohols.
abstract: The object of this research was improvement of prenyl alcohol production with squalene synthase-deficient mutant Saccharomyces cerevisiae ATCC 64031. On screening of many kinds of additives, we found that oils and detergents significantly enhanced the extracellular production of prenyl alcohols. Soybean oil showed the most prominent effect among the additives tested. Its effect was accelerated by a high concentration of glucose in the medium. The combination of these cultivation conditions led to the production of more than 28 mg/l of farnesol in the soluble fraction of the broth. The addition of these compounds to the medium was an effective method for large-scale production of prenyl alcohols with microorganisms.
authors.affiliation: Bio Research Laboratory, Toyota Motor Corporation, 1 Toyota-cho, Toyota, Aichi 471-8572, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Muramatsu
authors.forename: Chikara
authors.initials: C
authors.lastname: Ohto
authors.forename: Shusei
authors.initials: S
authors.lastname: Obata
authors.forename: Eiji
authors.initials: E
authors.lastname: Sakuradani
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D003902:Detergents / Q000494:pharmacology*; D005204:Farnesol / Q000378:metabolism*; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009387:Neoprene / Q000378:metabolism*; D010938:Plant Oils / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D003902:Detergents; D010938:Plant Oils; C016077:prenyl; D005204:Farnesol; D009387:Neoprene; D013186:Farnesyl-Diphosphate Farnesyltransferase