[Differential transcription of Bcl-2 and Bax through the cell cycle in prostate cancer cell line].
OBJECTIVE
To investigate the differential expression of apoptosis associated gene Bcl-2 and Bax through cell cycle and its possible clinical meaning.


METHODS
The prostate cancer cell line PC-3 was synchronized in M, G1, S and G2 phase using modified thymine deoxyriboside blockage and high pressure N2O technique. The efficiency of synchronization was detected by flow-cytometry. RT-PCR and Western blot methods were used to examine the expression of Bcl-2 and Bax in mRNA and protein level.


RESULTS
The synchronized rate of M, G1, S and G2 phase were 92.1%, 87.0%, 80.2% and 75.9% respectively. Bcl-2 was constitutively expressed through the cell cycle, but both the mRNA and protein expression level of Bcl-2 were very high in the G1 phase, dramatically decreased in M, S and G2 phase. The expression level of Bax had no change through the cell cycle.


CONCLUSIONS
Cell cycle could influence the expression level of Bcl-2 significantly but not Bax, these might have some clinical relevance.
Li
Xue-Song
XS
Department of Urology, Institute of Urology, Peking University First Hospital, Beijing 100034, China.
Gong
Kan
K
Sun
Guo-Feng
GF
Zhou
Li-Qun
LQ
He
Zhi-Song
ZS
Xin
Dian-Qi
DQ
Na
Yan-Qun
YQ
Liang
Yun-Yan
YY
Wang
Dai-Shu
DS
Guo
Ying-Lu
YL
D002453:Cell Cycle; D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000235:genetics
D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein