Heterologous expression of oxalate decarboxylase in Lactobacillus plantarum NC8.
Lactic acid bacteria (LABs) are being used as a probiotic very often for various enteric problems. Many genetically modified LABs are created by different workers for various novel applications. In this study we examine the expression of heterologous oxalate decarboxylase (oxdc) in Lactobacillus plantarum NC8. Generally, this enzyme is not present in Lactobacillus spp. Oxdc gene from Bacillus subtilis was polymerase chain reaction-amplified and cloned in a shuttle vector pSIP400 series, downstream of the inducible promoter, P(orfx). In the presence of an inducing peptide, Sakacin-P, the expression of OxdC was observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The cell-free extract and the purified protein from the recombinant LABs showed the presence of OxdC activity. The above recombinant LABs, with desired modifications, can be used as a possible probiotic for the degradation of intestinal dietary oxalate for preventing enteric hyperoxaluria.
Kolandaswamy
Anbazhagan
A
Department of Biochemistry, School of Biological Sciences, Madurai Kamaraj University, Madurai, TN, India. anbu50@gmail.com
George
Leema
L
Sadasivam
Selvam
S
D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D048191:Lactiplantibacillus plantarum / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D002262:Carboxy-Lyases; C021915:oxalate decarboxylase