[Association of cytokine gene polymorphisms with cachexia related to sporadic female breast cancer].
OBJECTIVE
To investigate the relation of the frequency of tumor necrosis factor (TNF)-alpha - 308, TNF-beta + 252, IL-1 beta + 3954, and IL-10 - 1082 gene polymorphisms to female breast cancer.


METHODS
Peripheral blood samples were collected from 102 breast cancer patients with cachexia and 120 breast cancer patients without cachexia. Biallelic polymorphisms were performed by analyzing the incision enzyme-digested DNA fragment obtained using PCR.


RESULTS
The allele frequencies of TNF-beta + 252, IL-1 beta + 3954, and IL-10 -1082 in the patients with cachexia were comparable with those of the patients without cachexia (all P > 0.05). The patients with cachexia showed a significantly higher prevalence of TNF2 than the patients without cachexia (20.6 % vs 10.0 %, P = 0.027). Logistic regression analysis indicated TNF2 as a risk factor for cachexia in breast cancer ( OR = 2.333, 95% CI: 1.085-5.017).


CONCLUSIONS
TNF2 plays an important role in the susceptibility of cachexia in breast cancer.
Li
Fu-nian
FN
Breast Disease Diagnosis and Treatment Centre, Hospital Affiliated to Qingdao University Medical College, Qingdao 266003, China. cngene@163.com
Zhang
Dian-liang
DL
Li
Yun-dong
YD
Jiang
Gui-mei
GM
D000328:Adult; D000368:Aged; D000483:Alleles; D001943:Breast Neoplasms / Q000150:complications*; D002100:Cachexia / Q000209:etiology / Q000235:genetics*; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D016015:Logistic Models; D008233:Lymphotoxin-alpha / Q000235:genetics; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016207:Cytokines; D053583:Interleukin-1beta; D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10