title: Immunosuppressive effect on T cell activation by interleukin-16- and interleukin-10-cDNA-double-transfected human squamous cell line.
abstract: It is well known that induction of immunotolerance with allogeneic skin transplantation is generally difficult. This study attempted to find an immunosuppressive protocol for skin allograft rejection involving interleukin-16 (IL-16) and interleukin-10 (IL-10), because both are known to inhibit mixed lymphocyte reaction (MLR). The data indicated that IL-16 enhanced the immunosuppressive effect of IL-10. IL-16-cDNA- and IL-10-cDNA-double-transfected squamous cell carcinoma cell line were used as an in vitro model and they produced more than 20 ng/ml of IL-16 and 100 pg/ml of IL-10 in the supernatant, which significantly inhibited MLR and also the activation of allogeneic lymphocytes, which were stimulated directly by allogeneic double-cDNA-transfectant cells. Thus allogeneic skin graft producing IL-16 and IL-10 might have a local immunosuppressive action that could prolong graft survival.
authors.affiliation: Division of Plastic Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan. ymatsu@sapmed.ac.jp
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fujita
authors.forename: Itaru
authors.initials: I
authors.lastname: Hirai
authors.forename: Hiroeki
authors.initials: H
authors.lastname: Sahara
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Torigoe
authors.forename: Kyori
authors.initials: K
authors.lastname: Ezoe
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Saito
authors.forename: William W
authors.initials: WW
authors.lastname: Cruikshank
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Yotsuyanagi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000276:immunology; D045744:Cell Line, Tumor; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology; D006085:Graft Survival / Q000235:genetics / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance / Q000502:physiology; D007165:Immunosuppression Therapy / Q000379:methods*; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D019410:Interleukin-16 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D016038:Skin Transplantation / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection / Q000379:methods
chemical_list: D019410:Interleukin-16; D016753:Interleukin-10