title: A novel low molecular weight phospholipase D from Streptomyces sp. CS684.
abstract: With the aim of isolating economically viable enzymes from a microbial source, a novel phospholipase D (PLD) was purified from Streptomyces sp. CS684 (PLD(684)). PLD(684) had molecular weight of 29 kDa, which makes it the second smallest PLD reported so far. The enzyme activity was optimum at pH 6 and 45 degrees C, and enhanced by various detergents. It was stable from pH 7 to 9 and at or below 45 degrees C when assayed after 40 h and 2h, respectively. The K(m) and V(max) values for phosphatidylcholine were 1.16 mM and 1453.74 micromol min(-1)mg(-1), respectively. It catalyzed the transphosphatidylation of glycerol, but not that of l-serine, myo-inositol or ethanolamine. Low molecular weight PLD(684) with transphosphatidylation activity may be utilized in the industrial production of rare and commercially important phospholipids.
authors.affiliation: Department of Pharmacy, College of Pharmacy, Chosun University, Dong-gu, Gwangju, Republic of Korea.
authors.forename: Jaya Ram
authors.initials: JR
authors.lastname: Simkhada
authors.forename: Hyo Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: So Young
authors.initials: SY
authors.lastname: Jang
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Hei Chan
authors.initials: HC
authors.lastname: Lee
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Yoo
mesh_terms: D004789:Enzyme Activation; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010739:Phospholipase D / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry*; D013302:Streptomyces / Q000378:metabolism*; D013696:Temperature
chemical_list: D010743:Phospholipids; D010739:Phospholipase D