Biosensor plates detect mitochondrial physiological regulators and mutations in vivo.
The measurement of mitochondrial activity in living cells is usually not straightforward, even though it is quite important in physiological and pathophysiological processes. We describe a high-throughput method for measurement of mitochondrial oxygen consumption in living cells, based on the Becton-Dickinson Biosensor plates.
Rolo
Anabela P
AP
Center for Neurosciences and Cell Biology, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal.
Palmeira
Carlos M
CM
Cortopassi
Gino A
GA
D015374:Biosensing Techniques / Q000379:methods*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D010101:Oxygen Consumption; D017354:Point Mutation / Q000235:genetics*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D024101:Mitochondrial Proteins; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone