title: Expression and roles of a xenopus head-forming gene homologue in human cancer cell lines.
abstract: Molecular mechanisms for both morphogenesis and carcinogenesis have frequently overlapped, and similar signaling pathways are often involved in these processes. Yamamoto et al. identified a novel protein that induces head formation in Xenopus (Yamamoto et al. Cell, 120, 223-225, 2005). This new protein, named Shisa, plays unique roles in head formation by suppressing the maturation processes of receptors for Wnt and FGF at the endoplasmic reticulum. Here, we have identified a human homologue of the shisa gene (hu-shisa-2), and analyzed its expression in various human cancer cell lines by real-time reverse transcription polymerase chain reaction. High levels of mRNA expression were observed in some neuroectoderm-derived human cancer cell lines and small cell lung cancer cell lines. Intracellular localization of hu-Shisa-2 protein was also analyzed, indicating that it is present in the endoplasmic reticulum. Over-expression of hu-Shisa-2 resulted in increased cell growth and invasion, suggesting that hu-Shisa-2 is involved in the evolution and/or progression of human cancers.
authors.affiliation: Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Yingsong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Akiko
authors.initials: A
authors.lastname: Tsuchida
authors.forename: Akihito
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Keiko
authors.initials: K
authors.lastname: Furukawa
authors.forename: Orie
authors.initials: O
authors.lastname: Tajima
authors.forename: Noriyo
authors.initials: N
authors.lastname: Tokuda
authors.forename: Shinichi
authors.initials: S
authors.lastname: Aizawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Urano
authors.forename: Kenji
authors.initials: K
authors.lastname: Kadomatsu
authors.forename: Koichi
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006257:Head / Q000196:embryology; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014981:Xenopus / Q000196:embryology* / Q000235:genetics*; D029867:Xenopus Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; C524790:SHISA2 protein, Xenopus; D029867:Xenopus Proteins