Technical considerations in using DNA microarrays to define regulons.
Transcription is the major regulatory target of gene expression in bacteria, and is controlled by many regulatory proteins and RNAs. Microarrays are a powerful tool to study the regulation of transcription on a genomic scale. Here we describe the use of transcription profiling and ChIP-chip to study transcriptional regulation in bacteria. Transcription profiling determines the outcome of regulatory events whereas ChIP-chip identifies the protein-DNA interactions that determine these events. Together they can provide detailed information on transcriptional regulatory systems.
Rhodius
Virgil A
VA
Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94143, USA. virgil.rhodius@ucsf.edu
Wade
Joseph T
JT
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011485:Protein Binding; D018085:Regulon / Q000235:genetics*
D001426:Bacterial Proteins