title: Initial crystallographic study of human PCNA in complex with a peptide containing the noncanonical PIP-box sequence of human DNA polymerase iota.
abstract: Human DNA polymerase iota (Poliota) is one of the Y-family DNA polymerases involved in translesion synthesis (TLS), which allows continued replication at damaged DNA templates. Poliota has a noncanonical PCNA-interacting protein box (PIP-box) within an internal region of the protein. Poliota activity is stimulated by PCNA binding through the noncanonical PIP-box. To clarify the interaction of PCNA with the noncanonical PIP-box of Poliota, PCNA and a Poliota peptide carrying the noncanonical PIP-box complex have been cocrystallized. The crystal belongs to space group C2, with unit-cell parameters a = 167.1, b = 68.7, c = 90.0 A, beta = 95.1 degrees . Structural analysis by molecular replacement is in progress.
authors.affiliation: International Graduate School of Arts and Sciences, Yokohama City University, Japan.
authors.forename: Asami
authors.initials: A
authors.lastname: Hishiki
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Tomo
authors.initials: T
authors.lastname: Hanafusa
authors.forename: Haruo
authors.initials: H
authors.lastname: Ohmori
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto
mesh_terms: D004249:DNA Damage; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014961:X-Ray Diffraction / Q000379:methods; D000094769:DNA Polymerase iota
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins; D004259:DNA-Directed DNA Polymerase; D000094769:DNA Polymerase iota; C000722070:POLI protein, human