title: Structural analysis of the interactions between paxillin LD motifs and alpha-parvin.
abstract: The adaptor protein paxillin contains five conserved leucine-rich (LD) motifs that interact with a variety of focal adhesion proteins, such as alpha-parvin. Here, we report the first crystal structure of the C-terminal calponin homology domain (CH(C)) of alpha-parvin at 1.05 A resolution and show that it is able to bind all the LD motifs, with some selectivity for LD1, LD2, and LD4. Cocrystal structures with these LD motifs reveal the molecular details of their interactions with a common binding site on alpha-parvin-CH(C), which is located at the rim of the canonical fold and includes part of the inter-CH domain linker. Surprisingly, this binding site can accommodate LD motifs in two antiparallel orientations. Taken together, these results reveal an unusual degree of binding degeneracy in the paxillin/alpha-parvin system that may facilitate the assembly of dynamic signaling complexes in the cell.
authors.affiliation: Laboratory of Molecular Biophysics, University of Oxford, Oxford OX1 3QU, United Kingdom.
authors.forename: Sonja
authors.initials: S
authors.lastname: Lorenz
authors.forename: Ioannis
authors.initials: I
authors.lastname: Vakonakis
authors.forename: Edward D
authors.initials: ED
authors.lastname: Lowe
authors.forename: Iain D
authors.initials: ID
authors.lastname: Campbell
authors.forename: Martin E M
authors.initials: ME
authors.lastname: Noble
authors.forename: Maria K
authors.initials: MK
authors.lastname: Hoellerer
mesh_terms: D000185:Actinin / Q000737:chemistry* / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D006801:Humans; D000090263:Leucine-Rich Repeat Proteins; D008840:Microfilament Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D051419:Paxillin / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology; D000096985:Calponins
chemical_list: D000185:Actinin; D000090263:Leucine-Rich Repeat Proteins; D008840:Microfilament Proteins; D051419:Paxillin; D011506:Proteins; C486590:PARVA protein, human; D000096985:Calponins