title: Desacyl ghrelin inhibits the orexigenic effect of peripherally injected ghrelin in rats.
abstract: Studies showed that the metabolic unlike the neuroendocrine effects of ghrelin could be abrogated by co-administered unacylated ghrelin. The aim was to investigate the interaction between ghrelin and desacyl ghrelin administered intraperitoneally on food intake and neuronal activity (c-Fos) in the arcuate nucleus in non-fasted rats. Ghrelin (13 microg/kg) significantly increased food intake within the first 30 min post-injection. Desacyl ghrelin at 64 and 127 microg/kg injected simultaneously with ghrelin abolished the stimulatory effect of ghrelin on food intake. Desacyl ghrelin alone at both doses did not alter food intake. Both doses of desacyl ghrelin injected separately in the light phase had no effects on food intake when rats were fasted for 12h. Ghrelin and desacyl ghrelin (64 microg/kg) injected alone increased the number of Fos positive neurons in the arcuate nucleus compared to vehicle. The effect on neuronal activity induced by ghrelin was significantly reduced when injected simultaneously with desacyl ghrelin. Double labeling revealed that nesfatin-1 immunoreactive neurons in the arcuate nucleus are activated by simultaneous injection of ghrelin and desacyl ghrelin. These results suggest that desacyl ghrelin suppresses ghrelin-induced food intake by curbing ghrelin-induced increased neuronal activity in the arcuate nucleus and recruiting nesfatin-1 immunopositive neurons.
authors.affiliation: Department of Medicine, Division Hepatology, Gastroenterology, and Endocrinology, Charité - Universitätsmedizin Berlin, Campus Virchow, Berlin, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Inhoff
authors.forename: Hubert
authors.initials: H
authors.lastname: Mönnikes
authors.forename: Steffen
authors.initials: S
authors.lastname: Noetzel
authors.forename: Andreas
authors.initials: A
authors.lastname: Stengel
authors.forename: Miriam
authors.initials: M
authors.lastname: Goebel
authors.forename: Q Thai
authors.initials: QT
authors.lastname: Dinh
authors.forename: Andrea
authors.initials: A
authors.lastname: Riedl
authors.forename: Norbert
authors.initials: N
authors.lastname: Bannert
authors.forename: Anna-Sophia
authors.initials: AS
authors.lastname: Wisser
authors.forename: Bertram
authors.initials: B
authors.lastname: Wiedenmann
authors.forename: Burghard F
authors.initials: BF
authors.lastname: Klapp
authors.forename: Yvette
authors.initials: Y
authors.lastname: Taché
authors.forename: Peter
authors.initials: P
authors.lastname: Kobelt
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects / Q000502:physiology*; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D004347:Drug Interactions; D004435:Eating / Q000187:drug effects / Q000502:physiology*; D005215:Fasting / Q000502:physiology; D054439:Ghrelin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D000080866:Nucleobindins; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D054439:Ghrelin; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D016760:Proto-Oncogene Proteins c-fos; C422650:ghrelin, des-n-octanoyl