[Transfection of human hyperplasia suppressor gene and hyperthermia have a synergistically apoptotic effect on colon cancer cell HT-29 in vitro].
OBJECTIVE
To investigate the suppression effect and apoptosis of the colon cell line HT-29 treated with adenovirus-expressed human hyperplasia suppressor gene (hHSG) and hyperthermia.


METHODS
Replication-defective recombinant adenovirus containing hHSG was constructed, and then infected into HT-29 cell line to detect its overexpression by western blot. The synergic effect with hyperthermia was observed, cell survival was assessed using Vi-CELL TMXR Cell Viability Analyzer, and cell apoptosis and cell cycle distribution were determined by flow cytometry.


RESULTS
After the cells were treated with 100 MOI (multiplicity of infection) Ad5-hHSG and heated at 42 degree C for 1 h, the number of viable cells decreased sharply, compared with single treatment with Ad5-hHSG or 42 degree C for 1 h (P < 0.01). The apoptotic rate of combined groups was significantly higher than that of Ad5-hHSG group or the heated group respectively (P < 0.01). Moreover, the combination treatment of Ad5-hHSG and 42 degree C for 1 h induced G2/M arrest.


CONCLUSIONS
Ad5-hHSG could increase the sensitivity of HT-29 to hyperthermia.
Wu
Li-na
LN
Department of Immunology, School of Basic Medical Sciences, Peking University, Peking University Human Disease Genomics Research Center, Beijing 100191, China.
Li
Zhi-xin
ZX
Lu
Tian
T
Ma
Teng
T
Qiu
Xiao-yan
XY
D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D020558:GTP Phosphohydrolases; D006358:Hot Temperature; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics*; D014162:Transfection
D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D020558:GTP Phosphohydrolases; C470018:MFN2 protein, human