Influence of phosphorus nutrition on growth and metabolism of Duo grass (Duo festulolium).
Use of suitable plants that can extract and concentrate excess P from contaminated soil serves as an attractive method of phytoremediation. Plants vary in their potential to assimilate different organic and inorganic P-substrates. In this study, the response of Duo grass (Duo festulolium) to variable rates of soil-applied potassium dihydrogen phosphate (KH(2)PO(4)) on biomass yield and P uptake were studied. Duo grown for 5 weeks in soil with 2.5, 5 and 7.5 g KH(2)PO(4) kg(-1) soil showed a significantly higher biomass and shoot P content of 8.3, 11.4 and 12.3g P kg(-1) dry weight respectively compared to plants that received no soil added P. Also, the ability of Duo to metabolize different forms of P-substrates was determined by growing them in sterile Hoagland's agar media with different organic and inorganic P-substrates, viz. KH(2)PO(4), glucose-1-phosphate (G1P), inositiol hexaphosphate (IHP), adenosine triphosphate (ATP) and adenosine monophosphate (AMP) for 2 weeks. Plants on agar media with different P-substrates also showed enhanced biomass yield and shoot P relative to no P control and the P uptake was in the order of ATP>KH(2)PO(4)>G1P>IHP=AMP>no P control. The activities of both phytase (E.C.3.1.3.26) and acid phosphatases (E.C.3.1.3.2) were higher in all the P received plants than the control. Duo grass is capable of extracting P from the soil and also from the agar media and thus it can serve as possible candidate for phytoextraction of high P-soil.
Priya
Padmanabhan
P
Department of Biology, Western Kentucky University, Bowling Green, KY 42101, USA.
Sahi
Shivendra V
SV
D010832:6-Phytase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism; D006109:Poaceae / Q000254:growth & development / Q000378:metabolism*; D017680:Potassium Compounds / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism
D010710:Phosphates; D017680:Potassium Compounds; D010758:Phosphorus; D000249:Adenosine Monophosphate; D010833:Phytic Acid; D000255:Adenosine Triphosphate; C013216:potassium phosphate; D000135:Acid Phosphatase; D010832:6-Phytase