title: Protective action of intravesical oxybutynin on bladder ultrastructure in rabbits with detrusor overactivity.
abstract: To evaluate the protective effect of intravesical oxybutynin on the ultrastructure of rabbits with detrusor overactivity (DO). Seventeen North Folk male rabbits were distributed into three groups: GI (n = 5) used as control, GII (n = 5), and GIII (n = 5) with DO. One animal from GII and one from GIII were excluded because they did not develop DO. In GIII, the animals were treated with daily intravesical application of 0.5 mg/Kg of oxybutynin for 30 days. Bladder weight was significantly higher in animals from GII and GIII as compared to GI. After 30 days, cystometric study revealed that vesical capacity was significantly decreased in GII and GIII. Detrusor pressure was significantly higher in GII. Electron microscopy showed increase of intercellular space, cell junctions and caveolae areas asymmetries, mitochondria and cellular degeneration in GII, while in GIII, these alterations have improved after a 30-day treatment. Animals treated with intravesical oxybutynin presented ultrastructural aspect similar to normal.
authors.affiliation: Department of Urology, School of Medicine, UNESP, Botucatu, Brazil.
authors.forename: Hamilto
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Paulo Roberto
authors.initials: PR
authors.lastname: Kawano
authors.forename: Karina Tuma
authors.initials: KT
authors.lastname: Balasteghin
authors.forename: Carlos Roberto
authors.initials: CR
authors.lastname: Padovani
authors.forename: João Luiz
authors.initials: JL
authors.lastname: Amaro
mesh_terms: D000283:Administration, Intravesical; D000818:Animals; D021941:Caveolae / Q000187:drug effects / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D007365:Intercellular Junctions / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008333:Mandelic Acids / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000648:ultrastructure; D018727:Muscarinic Antagonists / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D011817:Rabbits; D001743:Urinary Bladder / Q000187:drug effects / Q000648:ultrastructure*; D053201:Urinary Bladder, Overactive / Q000188:drug therapy* / Q000473:pathology
chemical_list: D008333:Mandelic Acids; D018727:Muscarinic Antagonists; C005419:oxybutynin