In vivo cloning of artificial DNA nanostructures.
Mimicking nature is both a key goal and a difficult challenge for the scientific enterprise. DNA, well known as the genetic-information carrier in nature, can be replicated efficiently in living cells. Today, despite the dramatic evolution of DNA nanotechnology, a versatile method that replicates artificial DNA nanostructures with complex secondary structures remains an appealing target. Previous success in replicating DNA nanostructures enzymatically in vitro suggests that a possible solution could be cloning these nanostructures by using viruses. Here, we report a system where a single-stranded DNA nanostructure (Holliday junction or paranemic cross-over DNA) is inserted into a phagemid, transformed into XL1-Blue cells and amplified in vivo in the presence of helper phages. High copy numbers of cloned nanostructures can be obtained readily by using standard molecular biology techniques. Correct replication is verified by a number of assays including nondenaturing PAGE, Ferguson analysis, endonuclease VII digestion, and hydroxyl radical autofootprinting. The simplicity, efficiency, and fidelity of nature are fully reflected in this system. UV-induced psoralen cross-linking is used to probe the secondary structure of the inserted junction in infected cells. Our data suggest the possible formation of the immobile four-arm junction in vivo.
Lin
Chenxiang
C
Department of Chemistry and Biochemistry and The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Rinker
Sherri
S
Wang
Xing
X
Liu
Yan
Y
Seeman
Nadrian C
NC
Yan
Hao
H
D003001:Cloning, Molecular; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D005363:Ficusin / Q000494:pharmacology; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation / Q000187:drug effects
D003432:Cross-Linking Reagents; D004247:DNA; D005363:Ficusin