Three galactose inducible promoters for use in C. neoformans var. grubii.
Cryptococcus neoformans is the causative agent of cryptococcal meningoencephalitis, most frequently occurring in immunocompromised individuals. There are three varieties of C. neoformans, var. grubii, var. neoformans, and var. gatti. Worldwide var. grubii is the most prevalent clinical isolate. However, few tools for the study of essential genes in var. grubii exist. Here we describe three endogenous inducible promoters for use in the study of this important opportunistic pathogen. We identified eight potential homologs of S. cerevisiae galactose genes in var. grubii. We found that GAL1, GAL7, and UGE2 were regulated by glucose and galactose and can be used successfully during mating. Our analysis indicated these promoters should prove to be excellent tools for analysis of genes in var. grubii.
Ruff
Jack A
JA
Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1100 S. Grand Boulevard, Saint Louis, MO 63104, USA.
Lodge
Jennifer K
JK
Baker
Lorina G
LG
D003455:Cryptococcus neoformans / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics
D005690:Galactose