Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species.
OBJECTIVE
To investigate the anti-oxidant properties of esculetin (6,7-dihydroxycoumarin) against H2O2-induced Chinese hamster lung fibroblast (V79-4) damage.


METHODS
The radical scavenging activity was assessed by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and intracellular reactive oxygen species (ROS). In addition, lipid peroxidation was assayed by the measure of related substances which react with thiobarbituric acid. The amount of carbonyl formation in protein was determined using a protein carbonyl ELISA kit. As well, cellular DNA damage was detected by Western blot and immunofluorescence image. Cell viability was assessed by 3-(4,5-dimethylthiazole-2- yl)-2,5-diphenyltetrazolium bromide assay.


RESULTS
Esculetin exhibited DPPH radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities. The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H2O2. Therefore, esculetin recovered cell viability exposed to H2O2.


CONCLUSIONS
Esculetin efficiently attenuated the oxidative stress induced cell damage via its anti-oxidant properties. As a result, esculetin may be useful in the development of functional food and raw materials of medicine.
Kim
So-hyung
SH
School of Medicine and Institute of Medical Science, Cheju National University, Jeju-si, Republic of Korea.
Kang
Kyoung-ah
KA
Zhang
Rui
R
Piao
Mei-jing
MJ
Ko
Dong-ok
DO
Wang
Zhi-hong
ZH
Chae
Sung-wook
SW
Kang
Sam-sik
SS
Lee
Keun-hwa
KH
Kang
Hee-kyoung
HK
Kang
Hyun-wook
HW
Hyun
Jin-won
JW
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D017665:Hydroxyl Radical / Q000737:chemistry; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates / Q000737:chemistry; D020011:Protective Agents; D050050:Protein Carbonylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*; D014468:Umbelliferones / Q000494:pharmacology*
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D016877:Oxidants; D010851:Picrates; D020011:Protective Agents; D017382:Reactive Oxygen Species; D014468:Umbelliferones; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; C007628:esculetin