Renewable and optically transparent electroactive indium tin oxide surfaces for chemoselective ligand immobilization and biospecific cell adhesion.
In this report, we show the successful transfer of a sophisticated electroactive immobilization and release strategy to an indium tin oxide (ITO) surface to generate (1) optically transparent, robust, and renewable surfaces, (2) inert surfaces that resist nonspecific protein adsorption and cell attachment, and (3) tailored biospecific surfaces for live-cell high-resolution fluorescence microscopy of cell culture. By comparing the surface chemistry properties on both ITO and gold surfaces, we demonstrate the ITO surfaces are superior to gold as a renewable surface, in robustness (durability), and as an optically transparent material for live-cell fluorescence microscopy studies of cell behavior. These advantages will make ITO surfaces a desired platform for numerous biosensor and microarray applications and as model substrates for various cell biological studies.
Luo
Wei
W
Department of Chemistry and Carolina Center for Genome Science, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA.
Westcott
Nathan P
NP
Pulsipher
Abigail
A
Yousaf
Muhammad N
MN
D000818:Animals; D001703:Biophysics / Q000379:methods; D002448:Cell Adhesion; D002627:Chemistry, Physical / Q000379:methods; D004563:Electrochemistry / Q000379:methods*; D005347:Fibroblasts / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008856:Microscopy, Fluorescence / Q000379:methods; D008956:Models, Chemical; D055095:Optics and Photonics; D051381:Rats; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry*
D008024:Ligands; D017971:Tin Compounds; C109984:indium tin oxide; D006046:Gold