Cross-kingdom amplification using bacteria-specific primers: complications for studies of coral microbial ecology.
PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.
Galkiewicz
Julia P
JP
University of South Florida, College of Marine Science, 140 7th Ave. S., St. Petersburg, Florida 33701, USA.
Kellogg
Christina A
CA
D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005057:Eukaryotic Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S