Kinetics of Wnt-driven beta-catenin stabilization revealed by quantitative and temporal imaging.
The Wnt/beta-catenin signal transduction pathway regulates a broad range of developmental processes. Aberrant activation of the Wnt pathway leads to cancer and degenerative diseases. beta-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation. This paper describes a multi-parametric method for quantitative analysis of cellular beta-catenin protein levels in a rapid and high-throughput manner. The assay offers temporally resolved detection of Wnt-stimulated accumulation of beta-catenin, simultaneously detecting cell number, and it sheds light onto the kinetics of posttranslational stabilization of beta-catenin.
Hannoush
Rami N
RN
Department of Protein Engineering, Genentech Inc., South San Francisco, California, United States of America. hannoush.rami@gene.com
D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D051379:Mice; D055550:Protein Stability; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000032:analysis* / Q000378:metabolism
D051153:Wnt Proteins; D051176:beta Catenin