title: Inhibition of the growth and metastasis of human colon cancer by restoration of RUNX3 expression in cancer cells.
abstract: Recent studies demonstrated an epigenetic inactivation of the runt-related transcription factor 3 (RUNX3) gene in human colon cancer. However, it remains unclear whether RUNX3 is tumor suppressive in colon cancer and, if so, the underlying molecular mechanisms of this activity are still unknown. In the present study, we sought to determine the level of RUNX3 expression in human colon tumor specimens and used an animal model of colon cancer to determine the impact of RUNX3 expression on tumor growth and metastasis. First, we analyzed RUNX3 expression in 83 human colon tumor specimens using immunohistochemical, reverse transcriptase-polymerase chain reaction, and Western blot analysis. RUNX3 mRNA and protein expression levels were consistently lower in tumor tissue specimens than in matched normal colon tissue specimens. Also, restoration of RUNX3 expression in colon cancer cells using gene transfer inhibited colon tumor growth and metastasis in our animal model, which was consistent with inhibition of colon tumor growth in vitro. Collectively, our clinical and experimental data support the notion that RUNX3 is a tumor suppressor in human colon cancer.
authors.affiliation: Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, PR China.
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Peng
authors.forename: Huamei
authors.initials: H
authors.lastname: Tang
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Wang
authors.forename: Chongzhi
authors.initials: C
authors.lastname: Zhou
authors.forename: Junwei
authors.initials: J
authors.lastname: Fan
authors.forename: Liwei
authors.initials: L
authors.lastname: Wang
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jia
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Le
authors.forename: Daoyan
authors.initials: D
authors.lastname: Wei
authors.forename: Keping
authors.initials: K
authors.lastname: Xie
mesh_terms: D000368:Aged; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics / Q000378:metabolism*; D044127:Epigenesis, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; D012333:RNA, Messenger; C493729:Runx3 protein, human; D025521:Tumor Suppressor Proteins