In vitro approaches to analysis of transcription termination.
Transcription termination is an important event in the transcription cycle that has been exploited in a variety of genetic regulatory mechanisms. Analysis of transcription termination is greatly facilitated by in vitro approaches. We describe a basic protocol for analysis of transcription termination in vitro, and include descriptions of parameters that can be modified for specific types of experimental questions.
Artsimovitch
Irina
I
Department of Microbiology and Center for RNA Biology, The Ohio State University, 484 W. 12th Avenue, Columbus, OH 43210, USA. artsimovitch.1@osu.edu
Henkin
Tina M
TM
D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D008957:Models, Genetic; D008967:Molecular Biology / Q000379:methods; D014158:Transcription, Genetic / Q000235:genetics*
D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases