title: RNA-binding properties of Dendrolimus punctatus tetravirus p17 protein.
abstract: The RNA-binding properties of the p17 protein of Dendrolimus punctatus tetravirus were analysed. We have demonstrated that p17 protein, a nonstructural protein with a potentially important role in viral replication, is a RNA-binding protein, which has not been previously described for any member of the family Tetraviridae. P17 protein was expressed in Escherichia coli and was used in UV cross-linking analysis, using a digoxigenin-UTP-labeled RNA probe and chemical cross-linking analysis. The analyses demonstrated that p17 protein could bind to RNA. When analysed for capacity of p17 to form oligomers, the protein could form dimers and tetramers. Furthermore, the circular dichroism spectrums of viral RNA 3'-UTR proved that their secondary structures were consistent with yeast tRNA.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Yangli
authors.initials: Y
authors.lastname: Zheng
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhou
authors.forename: Meijuan
authors.initials: M
authors.lastname: Lin
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Han
authors.forename: Xu
authors.initials: X
authors.lastname: Cao
authors.forename: Jiamin
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuanyang
authors.initials: Y
authors.lastname: Hu
mesh_terms: D020413:3' Untranslated Regions; D016679:Genome, Viral; D011485:Protein Binding; D012328:RNA Viruses / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins