title: [Effect of Helicobacter pylori-encoded CagA on biological behavior of gastric adenocarcinoma cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of Helicobacter pylori-encoded CagA on biological behavior of gastric adenocarcinoma AGS cells.
  
  
  METHODS
  With experiment-control system of the wild-type CagA positive strain and isogenic CagA negative mutant strain of Helicobacter pyroli (Hp) were used as control and experimental groups, respectively. The cell contact, migration and invasion were examined by light and electron microscopy and invasion assay.
  
  
  RESULTS
  The AGS cells infected by Hp strain with positive wild-type CagA showed more severely changed tight junction, wider intercellular space, loss of cell contacts, and higher migrating and invasive ability.
  
  
  CONCLUSIONS
  Hp CagA may lead to loss of cell contacting and higher migrating and invading ability of gastic cells, and accelerates the malignant progress of tumor.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Song
authors.forename: Hui-Xin
authors.initials: HX
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Bai
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Min-Hu
authors.initials: MH
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005110:Extracellular Space; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D007365:Intercellular Junctions / Q000648:ultrastructure*; D009154:Mutation; D013274:Stomach Neoplasms / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori