title: Detection of divinyl ether synthase in Lily-of-the-Valley (Convallaria majalis) roots.
abstract: Incubations of linoleic acid with cell-free preparations from Lily-of-the-Valley (Convallaria majalis L., Ruscaceae) roots revealed the presence of 13-lipoxygenase and divinyl ether synthase (DES) activities. Exogenous linoleic acid was metabolized predominantly into (9Z,11E,1'E)-12-(1'-hexenyloxy)-9,11-dodecadienoic (etheroleic) acid. Its identification was confirmed by the data of ultraviolet spectroscopy, mass spectra, (1)H NMR, COSY, catalytic hydrogenation. The isomeric divinyl ether (8E,1'E,3'Z)-12-(1',3'-nonadienyloxy)-8-nonenoic (colneleic) acid was detected as a minor product. Incubations with linoleic acid hydroperoxides revealed that 13-hydroperoxide was a preferential substrate, while the 9-hydroperoxide was utilized with lesser efficiency.
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Lobachevsky Street 2/31, P.O. Box 30, 420111 Kazan, Russia.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Ogorodnikova
authors.forename: Larisa R
authors.initials: LR
authors.lastname: Latypova
authors.forename: Fahima K
authors.initials: FK
authors.lastname: Mukhitova
authors.forename: Lucia S
authors.initials: LS
authors.lastname: Mukhtarova
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Grechkin
mesh_terms: D002851:Chromatography, High Pressure Liquid; D031405:Convallaria / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D019746:Intramolecular Oxidoreductases / Q000737:chemistry; D019787:Linoleic Acid / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System; D019787:Linoleic Acid; C100485:divinyl ether synthase; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase