Immunosuppressive effect on T cell activation by interleukin-16- and interleukin-10-cDNA-double-transfected human squamous cell line.
It is well known that induction of immunotolerance with allogeneic skin transplantation is generally difficult. This study attempted to find an immunosuppressive protocol for skin allograft rejection involving interleukin-16 (IL-16) and interleukin-10 (IL-10), because both are known to inhibit mixed lymphocyte reaction (MLR). The data indicated that IL-16 enhanced the immunosuppressive effect of IL-10. IL-16-cDNA- and IL-10-cDNA-double-transfected squamous cell carcinoma cell line were used as an in vitro model and they produced more than 20 ng/ml of IL-16 and 100 pg/ml of IL-10 in the supernatant, which significantly inhibited MLR and also the activation of allogeneic lymphocytes, which were stimulated directly by allogeneic double-cDNA-transfectant cells. Thus allogeneic skin graft producing IL-16 and IL-10 might have a local immunosuppressive action that could prolong graft survival.
Matsumoto
Yoshitaka
Y
Division of Plastic Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan. ymatsu@sapmed.ac.jp
Fujita
Tatsuya
T
Hirai
Itaru
I
Sahara
Hiroeki
H
Torigoe
Toshihiko
T
Ezoe
Kyori
K
Saito
Tamotsu
T
Cruikshank
William W
WW
Yotsuyanagi
Takatoshi
T
Sato
Noriyuki
N
D002294:Carcinoma, Squamous Cell / Q000276:immunology; D045744:Cell Line, Tumor; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology; D006085:Graft Survival / Q000235:genetics / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance / Q000502:physiology; D007165:Immunosuppression Therapy / Q000379:methods*; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D019410:Interleukin-16 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D016038:Skin Transplantation / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection / Q000379:methods
D019410:Interleukin-16; D016753:Interleukin-10