CCR5 deficiency aggravates crescentic glomerulonephritis in mice.
The chemokine receptor CCR5 is predominantly expressed on monocytes and Th1-polarized T cells, and plays an important role in T cell and monocyte recruitment in inflammatory diseases. To investigate the functional role of CCR5 in renal inflammation, we induced a T cell-dependent model of glomerulonephritis (nephrotoxic serum nephritis) in CCR5(-/-) mice. Induction of nephritis in wild-type mice resulted in up-regulation of renal mRNA expression of the three CCR5 chemokine ligands, CCL5 (15-fold), CCL3 (4.9-fold), and CCL4 (3.4-fold), in the autologous phase of the disease at day 10. The up-regulated chemokine expression was paralleled by infiltration of monocytes and T cells, followed by renal tissue injury, albuminuria, and loss of renal function. Nephritic CCR5(-/-) mice showed a 3- to 4-fold increased renal expression of CCL5 (61.6-fold vs controls) and CCL3 (14.1-fold vs controls), but not of CCL4, in comparison with nephritic wild-type mice, which was accompanied by augmented renal T cell and monocyte recruitment and increased lethality due to uremia. Furthermore, CCR5(-/-) mice showed an increased renal Th1 response, whereas their systemic humoral and cellular immune responses were unaltered. Because the CCR5 ligands CCL5 and CCL3 also act via CCR1, we investigated the effects of the pharmacological CCR1 antagonist BX471. CCR1 blockade in CCR5(-/-) mice significantly reduced renal chemokine expression, T cell infiltration, and glomerular crescent formation, indicating that increased renal leukocyte recruitment and consecutive tissue damage in nephritic CCR5(-/-) mice depended on functional CCR1. In conclusion, this study shows that CCR5 deficiency aggravates glomerulonephritis via enhanced CCL3/CCL5-CCR1-driven renal T cell recruitment.
Turner
Jan-Eric
JE
III. Medizinische Klinik, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany.
Paust
Hans-Joachim
HJ
Steinmetz
Oliver M
OM
Peters
Anett
A
Meyer-Schwesinger
Catherine
C
Heymann
Felix
F
Helmchen
Udo
U
Fehr
Susanne
S
Horuk
Richard
R
Wenzel
Ulrich
U
Kurts
Christian
C
Mittrücker
Hans-Willi
HW
Stahl
Rolf A K
RA
Panzer
Ulf
U
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000235:genetics / Q000276:immunology; D005921:Glomerulonephritis / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D007106:Immune Sera / Q000633:toxicity; D007074:Immunoglobulin G / Q000096:biosynthesis; D007668:Kidney / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D054389:Receptors, CCR1 / Q000037:antagonists & inhibitors / Q000502:physiology; D019713:Receptors, CCR5 / Q000172:deficiency* / Q000235:genetics*; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D014511:Uremia / Q000235:genetics / Q000276:immunology / Q000473:pathology
C515301:Ccr1 protein, mouse; D007106:Immune Sera; D007074:Immunoglobulin G; D054389:Receptors, CCR1; D019713:Receptors, CCR5