title: Regulation of the human PDZK1 expression by peroxisome proliferator-activated receptor alpha.
abstract: Although PDZK1 is a well-known adaptor protein, the mechanisms for its role in transcriptional regulation are largely unknown. The peroxisome proliferator-activated receptor alpha (PPARalpha) is a ligand-activated transcription factor that plays an important role in the regulation of lipid homeostasis. Previously, we established a tetracycline-regulated human cell line that can be induced to express PPARalpha and identified candidate target genes, one of which was PDZK1. In this study, we cloned and characterized the promoter region of the human pdzk1 gene and determined the PPAR response element. Finally, we demonstrate that endogenous PPARalpha regulates PDZK1 expression.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tachibana
authors.forename: Naohiko
authors.initials: N
authors.lastname: Anzai
authors.forename: Chihiro
authors.initials: C
authors.lastname: Ueda
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Katayama
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamasaki
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Kirino
authors.forename: Rika
authors.initials: R
authors.lastname: Takahashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishimoto
authors.forename: Hidenori
authors.initials: H
authors.lastname: Komori
authors.forename: Toshiya
authors.initials: T
authors.lastname: Tanaka
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Ueda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Arai
authors.forename: Juro
authors.initials: J
authors.lastname: Sakai
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
authors.forename: Takefumi
authors.initials: T
authors.lastname: Doi
mesh_terms: D024506:5' Flanking Region; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D047493:PPAR alpha / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; C484659:PDZK1 protein, human; D047493:PPAR alpha; D013752:Tetracycline