Fusarium populations on Chinese barley show a dramatic gradient in mycotoxin profiles.
We report on a large gene bank of Fusarium isolates established by a broad survey conducted in 2005 in which infected barley ears were collected in 23 counties of seven provinces and two municipalities along the Yangtze River in China. In total, 1,894 single spore isolates were obtained. The isolates were characterized at the species level by a newly developed and robust set of diagnostic primers based on single nucleotide polymorphisms (SNPs) among members of the F. graminearum clade. In addition, we determined their chemotype using previously described polymerase chain reaction (PCR) primers. The results showed that in all regions F. asiaticum was the predominant species causing Fusarium head blight (FHB) on barley in China (N = 1,706), while in the upper valleys of the Yangtze River also F. graminearum sensu stricto, F. meridionale, and F. proliferatum were found. Major differences in the chemotypes were found in the F. asiaticum populations, from very high to exclusive nivalenol (NIV) chemotypes in the mountainous upper valleys of the Yangtze River to predominantly deoxynivalenol (DON) chemotypes in the middle and lower valleys. In contrast to the F. asiaticum isolates from three counties in Sichuan province, which were largely NIV producers (278 of 291), F. graminearum isolates from these sampling sites were for the vast majority (27 of 28) DON producers, indicating that despite sharing the same habitat, these sympatric species apparently have unique mycotoxin chemotypes.
Yang
L
L
Institute for Plant Protection and Soil Sciences, Hubei Academy of Agricultural Sciences , Wuhan, China.
van der Lee
T
T
Yang
X
X
Yu
D
D
Waalwijk
C
C
D001483:Base Sequence; D044822:Biodiversity; D002681:China; D005670:Fusarium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014644:Genetic Variation; D005843:Geography; D001467:Hordeum / Q000382:microbiology*; D009183:Mycotoxins / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D013045:Species Specificity; D014255:Trichothecenes / Q000378:metabolism
D009183:Mycotoxins; D014255:Trichothecenes; C038405:nivalenol