Interaction between bacteriophage DMS3 and host CRISPR region inhibits group behaviors of Pseudomonas aeruginosa.
Bacteriophage infection has profound effects on bacterial biology. Clustered regular interspaced short palindromic repeats (CRISPRs) and cas (CRISPR-associated) genes are found in most archaea and many bacteria and have been reported to play a role in resistance to bacteriophage infection. We observed that lysogenic infection of Pseudomonas aeruginosa PA14 with bacteriophage DMS3 inhibits biofilm formation and swarming motility, both important bacterial group behaviors. This inhibition requires the CRISPR region in the host. Mutation or deletion of five of the six cas genes and one of the two CRISPRs in this region restored biofilm formation and swarming to DMS3 lysogenized strains. Our observations suggest a role for CRISPR regions in modifying the effects of lysogeny on P. aeruginosa.
Zegans
Michael E
ME
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA.
Wagner
Jeffrey C
JC
Cady
Kyle C
KC
Murphy
Daniel M
DM
Hammond
John H
JH
O'Toole
George A
GA
D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D018441:Biofilms; D002455:Cell Division; D003001:Cloning, Molecular; D022801:Complementarity Determining Regions / Q000235:genetics; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D016680:Genome, Bacterial; D016679:Genome, Viral; D008242:Lysogeny; D010933:Plankton / Q000254:growth & development; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000821:virology*; D020449:Repetitive Sequences, Amino Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology
D022801:Complementarity Determining Regions; D017931:DNA Primers; D004279:DNA, Viral; D014764:Viral Proteins