Monoclonality and oligoclonality of T cell receptor beta gene in angioimmunoblastic T cell lymphoma.
Angioimmunoblastic T cell lymphoma (AILT) is an aggressive T cell lymphoma with an incidence of approximately 1-2% of all non-Hodgkin lymphoma. The detection of clonal T cell receptor (TCR) gene rearrangements helps in the diagnosis of T cell malignancies such as AILT, where morphological and immunohistological investigations are not always sufficient to reach a definitive diagnosis. TCR beta (TCRB) and TCR gamma (TCRG) gene rearrangements were analysed from 17 WHO-defined cases of AILT by PCR for the presence of TCR clonality. TCRB gene rearrangements were sequenced to identify molecular signature(s) common among this patient group. Monoclonal and oligoclonal TCRB and TCRG gene rearrangements were detected in all cases. BV17S1 was slightly over-represented compared to the use of other Vbeta gene segments; however, no preferential usage of J gene segment(s) was detected. The results of this study emphasise that TCR clonality and oligoclonality is a diagnostic feature of AILT and that BV17S1 is over-represented with no other common molecular findings.
Shah
Z H
ZH
Department of Molecular Pathology, Southampton General Hospital, Southampton, UK. zs516@york.ac.uk
Harris
S
S
Smith
J L
JL
Hodges
E
E
D004273:DNA, Neoplasm / Q000235:genetics; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000175:diagnosis / Q000235:genetics*; D016399:Lymphoma, T-Cell / Q000175:diagnosis / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*
D004273:DNA, Neoplasm; D016693:Receptors, Antigen, T-Cell, alpha-beta