title: MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1.
abstract: Rap1 and Rap2 are similar Ras-like G proteins but perform distinct functions. By the affinity chromatography/mass-spectrometry approach and the yeast two-hybrid screening, we identified Misshapen/NIKs-related kinase (MINK) as a novel Rap2-interacting protein that does not interact with Rap1 or Ras. MINK is a member of the STE20 group of mitogen-activated protein kinase kinase kinase kinases. The interaction between MINK and Rap2 was GTP-dependent and required Phe39 within the effector region of Rap2; the corresponding residue in Rap1 and Ras is Ser. MINK was enriched in the brain, and both MINK and its close relative, Traf2- and Nck-interacting kinase (TNIK), interacted with a postsynaptic scaffold protein containing tetratricopeptide repeats, ankyrin repeats and a coiled-coil region (TANC1) and induced its phosphorylation, under control of Rap2 in cultured cells. These are novel actions of MINK and TNIK, and consistent with a role of MINK as a Rap2 effector in the brain.
authors.affiliation: Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Nonaka
authors.affiliation: Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Takei
authors.affiliation: Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Umikawa
authors.affiliation: Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Oshiro
authors.affiliation: Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kuninaka
authors.affiliation: Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
authors.forename: Maitsetseg
authors.initials: M
authors.lastname: Bayarjargal
authors.affiliation: Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Asato
authors.affiliation: Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Yamashiro
authors.affiliation: Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan; Unit for Molecular Neurobiology of Learning and Memory, Initial Research Project, Okinawa Institute of Science and Technology, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Uechi
authors.affiliation: Unit for Molecular Neurobiology of Learning and Memory, Initial Research Project, Okinawa Institute of Science and Technology, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Endo
authors.affiliation: Department of Neuroplasticity, Shinshu University Graduate School of Medicine, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan. Electronic address: kariya@med.u-ryukyu.ac.jp.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Kariya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D023902:Chromosome Pairing; D003435:Crotalid Venoms / Q000235:genetics / Q000378:metabolism*; D000081410:Germinal Center Kinases; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D020668:rap GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D003435:Crotalid Venoms; D000081410:Germinal Center Kinases; D037181:Lectins, C-Type; C499875:TANC1 protein, rat; C533352:MINK7 protein, mouse; D017346:Protein Serine-Threonine Kinases; C000631454:TNIK protein, human; C116756:RAP2A protein, human; D020668:rap GTP-Binding Proteins