Jakmip1 is expressed upon T cell differentiation and has an inhibitory function in cytotoxic T lymphocytes.
Jakmip1 belongs to a family of three related genes encoding proteins rich in coiled-coils. Jakmip1 is expressed predominantly in neuronal and lymphoid cells and colocalizes with microtubules. We have studied the expression of Jakmip1 mRNA and protein in distinct subsets of human primary lymphocytes. Jakmip1 is absent in naive CD8(+) and CD4(+) T lymphocytes from peripheral blood but is highly expressed in Ag-experienced T cells. In cord blood T lymphocytes, induction of Jakmip1 occurs upon TCR/CD28 stimulation and parallels induction of effector proteins, such as granzyme B and perforin. Further analysis of CD8(+) and CD4(+) T cell subsets showed a higher expression of Jakmip1 in the effector CCR7(-) and CD27(-) T cell subpopulations. In a gene expression follow-up of the development of CMV-specific CD8(+) response, Jakmip1 emerged as one of the most highly up-regulated genes from primary infection to latent stage. To investigate the relationship between Jakmip1 and effector function, we monitored cytotoxicity of primary CD8(+) T cells silenced for Jakmip1 or transduced with the full-length protein or the N-terminal region. Our findings point to Jakmip1 being a novel effector memory gene restraining T cell-mediated cytotoxicity.
Libri
Valentina
V
Cytokine Signaling Unit, Department of Immunology, Centre National de la Recherche Scientifique Unité de Recherche Associée 1961, Institut Pasteur, 75724 Paris, France.
Schulte
Dörte
D
van Stijn
Amber
A
Ragimbeau
Josiane
J
Rogge
Lars
L
Pellegrini
Sandra
S
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D016923:Cell Death / Q000235:genetics / Q000276:immunology; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D003587:Cytomegalovirus / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007156:Immunologic Memory / Q000235:genetics; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000378:metabolism* / Q000821:virology
D048868:Adaptor Proteins, Signal Transducing; D006131:Growth Inhibitors; C484851:JAKMIP1 protein, human; D016601:RNA-Binding Proteins; D011948:Receptors, Antigen, T-Cell; D017493:Leukocyte Common Antigens