Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines.
BACKGROUND
Lactic acid bacteria (LAB) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as anti-tumor activity. The aim of the present work was to study the growth inhibition of tumor cells by butanol extract of Bifidobacterium adolescentis isolated from healthy young Koreans.


METHODS
The anti-proliferative activity of B. adolescentis isolates was assessed by XTT assays on three human colon cancer cell lines (Caco-2, HT-29, and SW480). The effects of B. adolescentis SPM0212 butanol extract on tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) production were tested using the murine macrophage RAW 264.7 cell line.


RESULTS
The butanol extract of B. adolescentis SPM0212 dose-dependently inhibited the growth of Caco-2, HT-29, and SW480 cells by 70%, 30%, and 40%, respectively, at 200 microg/mL. Additionally, the butanol extract of B. adolescentis SPM0212 induced macrophage activation and significantly increased the production of TNF-alpha and NO, which regulate immune modulation and are cytotoxic to tumor cells.


CONCLUSIONS
The butanol extract of B. adolescentis SPM0212 increased activity of the host immune system and may improve human health by helping to prevent colon cancer as a biological response modifier.
Lee
Do Kyung
DK
Department of Pharmacy, Sahmyook University, Seoul 139-742, Republic of Korea. 015790@hanmail.net
Jang
Seok
S
Kim
Mi Jin
MJ
Kim
Jung Hyun
JH
Chung
Myung Jun
MJ
Kim
Kyung Jae
KJ
Ha
Nam Joo
NJ
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D001644:Bifidobacterium / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000187:drug effects
D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide