[Development of a protocol on pulsed field gel electrophoresis analysis for Streptococcus suis].
OBJECTIVE
To develop a PFGE protocol for Streptococcus suis.


METHODS
We developed and optimized a PFGE protocol for S. suis, in terms of plug preparation, choice of restriction endonucleases and optimized electrophoresis parameters. By analyzing the genome sequences of S. suis P1/7 with Mapdraw of DNAStar, we found three restriction enzymes, Swa I, Sma I and Apa I, were more suitable than others.


RESULTS
Analysis of 100 isolates of S. suis including 34 of 35 serotypes identified, 59, 53 and 43 patterns were obtained from Swa I, Sma I and Apa I restriction, respectively. The enzyme Swa I had the greatest power for discrimination ability.


CONCLUSIONS
By optimization of the protocol at various conditions, a rapid, reproducible, economic and practical PFGE method for S. suis was developed.
Wang
Li-Li
LL
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Ye
Chang-Yun
CY
Xu
Yan-Mei
YM
Cui
Zhi-Gang
ZG
Jing
Huai-Qi
HQ
Jin
Dong
D
Du
Hua-Mao
HM
Zhang
Shou-Yin
SY
Bai
Xue-Mei
XM
Zhao
Ai-Lan
AL
Xu
Jian-Guo
JG
D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D012703:Serotyping; D017011:Streptococcus suis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine
D004269:DNA, Bacterial