[Identification of the hemolytic transfusion reaction caused by lewis antibody using serological and molecular biological methods].
To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient's serum were identified using panel cells and Le (a-b-) phenotype cells, patient phenotype was identified by using anti-Le(a) and anti-Le(b) blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly. The results showed that both IgM anti-Le(a) and anti-Le(b) antibodies were detected in patient's serum. Red cells was typed as Le (a-b-) phenotype and the FUT3 genotype was homozygote for non-functional le(59, 508) alleles. In conclusion, anti-Le(b) antibody can result in hemolytic transfusion reaction, FUT3 gene is homozygous for le(59, 508) allele resulting in Le (a-b-) phenotype.
Hong
Xiao-Zhen
XZ
Institute of Transfusion Medicine, Ministry of Health, Hangzhou, 310006, Zhejiang Province, China.
Xu
Xian-Guo
XG
Zhu
Fa-Ming
FM
Ma
Kai-Rong
KR
Yan
Li-Xing
LX
D000328:Adult; D000906:Antibodies / Q000009:adverse effects* / Q000276:immunology*; D001788:Blood Grouping and Crossmatching; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics; D005838:Genotype; D006402:Hematologic Diseases; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D012699:Serology; D065227:Transfusion Reaction
D000906:Antibodies; D007983:Lewis Blood Group Antigens; D005647:Fucosyltransferases; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase