title: Efficient bioethanol production from xylose by recombinant saccharomyces cerevisiae requires high activity of xylose reductase and moderate xylulokinase activity.
abstract: We varied the promoter strength of xylose reductase (XR) gene and the copy number of xylulokinase (XK) gene to determine how XR and XK activities affect the xylose-fermenting abilities of recombinant Saccharomyces cerevisiae expressing xylitol dehydrogenase (XDH). The most enhanced ethanol yield and lowered xylitol yield occurred in strain I-PGK/AUR, which has high activity of both XR and XDH and moderate XK activity.
authors.affiliation: Biomass Technology Research Center (BTRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Matsushika
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sawayama
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism*; D001674:Bioelectric Energy Sources; D004789:Enzyme Activation; D000431:Ethanol / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D000431:Ethanol; D014994:Xylose; D000449:Aldehyde Reductase; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase