title: Ovarian stimulation to cryopreserve fertilized oocytes in cancer patients can be started in the luteal phase.
abstract: OBJECTIVE
  To analyze if oocytes can be obtained in all patients before cancer treatment within 2 weeks by initiating ovarian stimulation during the follicular or luteal phase.
  
  
  METHODS
  Prospective controlled multicenter trial.
  
  
  METHODS
  Four university-based centers.
  
  
  METHODS
  Forty cancer patients before chemotherapy.
  
  
  METHODS
  Twenty-eight patients were stimulated with gonadotropins in the follicular phase (group I). In 12 patients (group II), ovarian stimulation was initiated in the luteal phase, and these received GnRH antagonists and recombinant FSH. In 14 patients, 143 oocytes were further processed for fertilization by intracytoplasmic sperm injection (ICSI).
  
  
  METHODS
  Number of oocytes aspirated after ovarian stimulation, cumulative FSH/hMG dosage, viability and maturity of oocytes, and fertilization rate by ICSI.
  
  
  RESULTS
  Patients in group I (age 27.6 +/- 4.9 yrs) were stimulated on average for 10.6 days, and patients in group II (age 31.2 +/- 5.7 yrs) for 11.4 days. Total amount of FSH was on average 2,255 IU (I) and 2,720 IU (II) per patient. Average and median numbers of aspirated oocytes were, respectively, 13.1 and 11.5 (I) versus 10.0 and 8.5 (II); 83.7% (I) and 80.4% (II) of the oocytes were mature and viable and could be treated by ICSI. Fertilization rate was 61.0% (I) versus 75.6% (II).
  
  
  CONCLUSIONS
  This pilot study suggests that oocytes can be obtained before cancer treatment efficiently irrespective of the phase of the menstrual cycle.
authors.affiliation: Department of Gynecologic Endocrinology and Reproductive Medicine, Women's University Hospital, University of Heidelberg, Heidelberg, Germany. Electronic address: michael.von.wolff@med.uni-heidelberg.de.
authors.forename: Michael
authors.initials: M
authors.lastname: von Wolff
authors.affiliation: Gynecologic Endocrinology and Reproductive Medicine, Women's Hospital, Ludwig-Maximilians-University of Munich, Campus Grosshadern, Grosshadern, Germany.
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Thaler
authors.affiliation: Department of Obstetrics and Gynecology, University of Wuerzburg, Wuerzburg, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Frambach
authors.affiliation: Department of Gynecologic Endocrinology and Reproduction, Women's Hospital of St. Gallen, St. Gallen, Switzerland.
authors.forename: Cosima
authors.initials: C
authors.lastname: Zeeb
authors.affiliation: Women's Hospital, University of Tuebingen, Tuebingen, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Lawrenz
authors.affiliation: Department of Gynecologic Endocrinology and Reproductive Medicine, Women's University Hospital, University of Heidelberg, Heidelberg, Germany.
authors.forename: Roxana M
authors.initials: RM
authors.lastname: Popovici
authors.affiliation: Department of Gynecologic Endocrinology and Reproductive Medicine, Women's University Hospital, University of Heidelberg, Heidelberg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Strowitzki
mesh_terms: D000293:Adolescent; D000328:Adult; D015925:Cryopreservation / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D008183:Luteal Phase / Q000502:physiology*; D009369:Neoplasms / Q000503:physiopathology / Q000534:rehabilitation; D054315:Oocyte Retrieval / Q000379:methods; D010062:Ovulation Induction / Q000379:methods*; D010865:Pilot Projects; D011247:Pregnancy; D018873:Pregnancy Rate; D055815:Young Adult; D015053:Zygote