title: Influences of culture temperature on the growth, lipid content and fatty acid composition of Aurantiochytrium sp. Strain mh0186.
abstract: The growth, lipid content, and fatty acid composition of Aurantiochytrium sp. strain mh0186 at different temperatures were investigated. Strain mh0186 grew well at 15-30 degrees C, but weakly at 10 degrees C. The biomass at 15-30 degrees C was significantly higher than at 10 and 35 degrees C, and the total lipid at 15-35 degrees C was significantly higher than that at 10 degrees C. The amount of DHA in the total fatty acid was highest at 10 degrees C and decreased in response to temperature increase. The content of DHA (mg/g-dry cell weight) at 15-30 degrees C were significantly higher than those at 35 degrees C and those at 15-25 degrees C were significantly higher than those at 10 and 35 degrees C. The DHA yield at 15-35 degrees C was significantly higher than those at 10 and 35 degrees C. Unsaturation of fatty acid was regulated by temperature and was enhanced in response to temperature decrease. The ratio of DHA to DPA varied at different temperatures.
authors.affiliation: Laboratory of Marine Bioscience, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan.
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Taoka
authors.forename: Naoki
authors.initials: N
authors.lastname: Nagano
authors.forename: Yuji
authors.initials: Y
authors.lastname: Okita
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Izumida
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sugimoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D018533:Biomass; D003470:Culture Media; D056890:Eukaryota / Q000737:chemistry* / Q000254:growth & development*; D005227:Fatty Acids / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D009792:Oceans and Seas; D013696:Temperature
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids