Induction of fatty acid-binding protein 3 in brown adipose tissue correlates with increased demand for adaptive thermogenesis in rodents.
We investigated the contribution of fatty acid-binding protein 3 (FABP3) to adaptive thermogenesis in brown adipose tissue (BAT) in rodents. The expression of FABP3 mRNA in BAT was regulated discriminatively in response to alteration of the ambient temperature, which regulation was similar and reciprocal to the regulation of uncoupling protein 1 (UCP1) and leptin, respectively. FABP3 expression in the BAT was significantly higher in the UCP1-knockout (KO) mice than in the wild-type ones, and these KO mice showed a higher clearance rate of free fatty acid from the plasma. In addition, FABP3 expression in the BAT was increased greatly with the development of diet-induced obesity in mice. These results indicate that the induction of FABP3 in BAT correlates with an increased demand for adaptive thermogenesis in rodents. FABP3 appears to be essential for accelerating fatty acid flux and its oxidation through UCP1 activity for non-shivering thermogenesis in BAT.
Yamashita
Hitoshi
H
Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai 487-8501, Japan. Electronic address: hyamashi@isc.chubu.ac.jp.
Wang
Zuocheng
Z
Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alta., Canada T6G 2H7.
Wang
Youxue
Y
Department of Surgery, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.
Segawa
Masahiko
M
Department of Preventive Medicine and Public Health, National Defense Medical College, Tokorozawa 359-8513, Japan.
Kusudo
Tatsuya
T
Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai 487-8501, Japan.
Kontani
Yasuhide
Y
Department of Food Science for Health, Minami-Kyushu University, Miyazaki 880-0032, Japan.
D000222:Adaptation, Physiological / Q000235:genetics; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000502:physiology*; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D024101:Mitochondrial Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D022722:Thermogenesis / Q000235:genetics; D000071256:Uncoupling Protein 1
C493409:Fabp3 protein, mouse; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604372:Ucp1 protein, mouse; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1