Increased metabolite concentration in migraine rat model by proton MR spectroscopy in vivo and ex vivo.
OBJECTIVE
To investigate brain metabolite change in rat migraine model by proton magnetic resonance spectroscopy (1H-MRS) in vivo and ex vivo.


METHODS
18 Adult SD rats were enrolled in the study. After nitroglycerin administration, the migraine rat model was established according to ethology evaluation. 1H-MRS was performed at 3T MR scanner in vivo and 14.7T Bruker MR spectrometer ex vivo. Area of peak for different metabolite material was obtained and compared by using independent-T test. The significance level was set at p<0.05.


RESULTS
In thalami of experimental group, areas of peak for Cho, Cr and Glu both in vivo and ex vivo were higher than in control group(p<0.05). In contrast, there was no statistical difference in cerebellum.


CONCLUSIONS
The elevated Cho and Cr spectral pattern were found in rat migraine model. Neuron activity in thalamus is significantly changeable during attacks.
Ma
Zhe
Z
Department of Ultrasonography, QiLu Hospital of Shandong University, Jinan City, Shandong Province, China.
Wang
Sheng-jun
SJ
Li
Chuan-fu
CF
Ma
Xiang-xing
XX
Gu
Tao
T
D000818:Animals; D001224:Aspartic Acid / Q000032:analysis; D002531:Cerebellum / Q000378:metabolism; D002794:Choline / Q000032:analysis; D003401:Creatine / Q000032:analysis; D004195:Disease Models, Animal; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008881:Migraine Disorders / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D011522:Protons; D051381:Rats; D017207:Rats, Sprague-Dawley; D013057:Spectrum Analysis; D013788:Thalamus / Q000378:metabolism
D011522:Protons; D001224:Aspartic Acid; D003401:Creatine; D002794:Choline