title: Expression of orotate phosphoribosyltransferase in colorectal carcinoma: an immunohistochemical analysis in several components of neoplastic lesions.
abstract: The purpose of this study was to evaluate the pattern of the expression of orotate phosphoribosyltransferase (OPRT) in several components of colorectal carcinoma (CRC). Fifty-six surgically-resected samples of CRC were subjected to immunohistochemistry with a polyclonal anti-OPRT antibody. Grading was performed independently for several components of CRC, including mucosal carcinoma lesions (n=56), infiltrative lesions (n=53), lymphovascularly invasive lesions (n=34) and metastatic lymph nodes (n=17). The expression of OPRT in mucosal carcinoma and infiltrative lesions correlated significantly only with the presence of lymphovascular invasion (p=0.0007 and <0.0001, respectively). The frequency of OPRT expression in mucosal carcinoma, infiltrative and lymphovascularly invasive lesions as well as metastatic lymph nodes was 32.1, 69.8, 88.2 and 88.8%, respectively. In addition, nuclear staining of OPRT was observed in metastatic lymph nodes and lymphovascularly invasive lesions. Our results suggest that OPRT is involved in the invasion and metastasis of CRC.
authors.affiliation: Department of Surgical Oncology, Research Institution for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan. ysanadasurg@hotmail.com
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sanada
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yoshida
authors.forename: Jun
authors.initials: J
authors.lastname: Hihara
authors.forename: Morihito
authors.initials: M
authors.lastname: Okada
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D062445:Claudin-1; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis; D008875:Middle Aged; D009962:Orotate Phosphoribosyltransferase / Q000096:biosynthesis*
chemical_list: C568108:CLDN1 protein, human; D062445:Claudin-1; D008565:Membrane Proteins; D009962:Orotate Phosphoribosyltransferase