title: Safety evaluation of a canine hepatitis DNA vaccine.
abstract: DNA vaccines are a novel disease prevention methodology, however their safety has not been well described. We evaluated the safety and efficacy of the DNA vaccine pVAX1-CpG-Loop against infectious canine hepatitis. We demonstrated that pVAX1-CpG-Loop could not be recovered from tissues of vaccinated mice nor from F1 progeny and following vaccination there were no apparent changes in hematologic markers compared to unvaccinated controls. Most important, vaccinated mice were protected from viral challenge. The only detectable effects of the vaccination were elevated AST levels 4 weeks post vaccination and liver lymphocyte infiltration and hydropic degeneration which normalized 6 months later.
authors.affiliation: Department of Molecular Biology, Hebei Key Lab of Laboratory Animal, Hebei Medical University, Shijiazhuang 050017, People's Republic of China.
authors.forename: Fu Ying
authors.initials: FY
authors.lastname: Liu
authors.forename: Rui Min
authors.initials: RM
authors.lastname: Guo
authors.forename: Long
authors.initials: L
authors.lastname: Zheng
authors.forename: Yi Ming
authors.initials: YM
authors.lastname: Lu
authors.forename: Hongyu
authors.initials: H
authors.lastname: You
authors.forename: Song
authors.initials: S
authors.lastname: Shuxia
authors.forename: Jun Xia
authors.initials: JX
authors.lastname: Wang
authors.forename: Shu Han
authors.initials: SH
authors.lastname: Sun
mesh_terms: D006516:Adenoviruses, Canine / Q000276:immunology*; D000818:Animals; D017931:DNA Primers / Q000276:immunology; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D004285:Dogs; D005260:Female; D018095:Germ-Line Mutation; D006522:Hepatitis, Infectious Canine / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D007115:Immunization Schedule; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D019444:Vaccines, DNA / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000009:adverse effects / Q000276:immunology* / Q000627:therapeutic use*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines