Potential chemoprevention effect of dietary fucoxanthin on urinary bladder cancer EJ-1 cell line.
Bladder cancer is a common but serious malignancy. It is widely accepted that chemoprevention may be an effective way to decrease the rate of recurrence and morbidity. We first determined antigrowth and apoptosis-induction activity of fucoxanthin from dietary Laminaria japonica against EJ-1 human bladder cancers. Fucoxanthin significantly reduced the cell viability in a dose- and time-dependent manner. The induction of apoptosis in EJ-1 cells was characterized by morphological changes, DNA ladder, and increased percentage of hypodiploid cells, activating caspase-3 activity. The ratio of apoptotic cells reached >93% after treatment for 72 h with 20 microM fucoxanthin. The findings obtained indicate that fucoxanthin may act as a chemopreventive and/or chemotherapeutic carotenoid in bladder cancer cells by modulating cell viability.
Zhang
Zhenya
Z
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
Zhang
Peijuan
P
Hamada
Michito
M
Takahashi
Satoru
S
Xing
Guoqing
G
Liu
Jiqiang
J
Sugiura
Norio
N
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D018890:Chemoprevention; D004032:Diet; D005434:Flow Cytometry; D006801:Humans; D017382:Reactive Oxygen Species; D001749:Urinary Bladder Neoplasms / Q000517:prevention & control*; D024341:Xanthophylls / Q000494:pharmacology*
D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; D024341:Xanthophylls; C025164:fucoxanthin