[Genetic polymorphism of k-casein gene exon 4 and its cor-relation with milk production traits in Chinese Holsteincows.].
K-casein gene was regarded as a candidate gene for milk production traits of cows. In this study, a 779 bp fragment of k-casein gene of Chinese Holstein was amplified by polymerase chain reaction (PCR), the polymorphisms of three loci of k-casein gene were detected by PCR-RFLP with restriction endonuclease Taq, Hind, Pst. After sequencing, T/C single nucleotide polymorphism (SNP) was identified at nucleotide 10 891C/A SNP was identified at nucleotide 10 927 and G/A SNP was identified at nucleotide 10 988 in exon4 of k-casein gene. Both alleles (A and B) of three loci were found in the population that showed low polymorphism. The gene frequencies of A and B were 86.03% and 13.97%, respectively. The genotype frequencies of AA, AB, and BB were 73.71%, 24.63%, and 1.66%, respectively. Statistical results of c2 test indicated that three polymorphism sites in the population fitted with Hardy-Weinberg equilibrium (P > 0.05). Meanwhile, the effect of polymorphism of k-casein gene on milk production traits was analyzed. The results indicated that in the three loci, the different genotype of k-casein gene had no significant influence on milk yield and milk protein percent (P > 0.05). The cows with genotypes BB and AB showed higher milk fat percent than those with genotype AA ( P < 0.05 ) ; with genotype AB showed higher fat protein ratio than those with genotype AA ( P < 0.05 ). The polymorphism of the three loci in the experimental population is closely linked. The conclusion is that k-casein B allele can be used as the molecular genetic markers of modifying milk fat percent in Chinese Holstein cows.
Ju
Zhi-Hua
ZH
1. Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan 250100, China; 2. Life Science College, Shandong Normal University, Jinan 250014, China E-mail: juzhihua283@163.com.
Li
Qiu-Ling
QL
Wang
Hong-Mei
HM
Li
Jian-Bin
JB
An
Li-Guo
LG
Yang
Gui-Wen
GW
Zhong
Ji-Feng
JF
Wang
Chang-Fa
CF
D000818:Animals; D002364:Caseins; D005091:Exons; D005787:Gene Frequency; D005838:Genotype; D008892:Milk / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length
D002364:Caseins