title: Optimization of medium components for increased production of C-phycocyanin from Phormidium ceylanicum and its purification by single step process.
abstract: Phycocyanin is a major protein produced by cyanobacteria, but very few phycocyanin-producing strains have been reported. In the present study, response surface methodology (RSM) involving a central composite design for four factors was successfully employed to optimize medium components for increased production of phycocyanin from Phormidium ceylanicum. The production of phycocyanin and interactions between sodium nitrate, calcium chloride, trace metal mix and citric acid stock were investigated and modeled. Under optimized condition P. ceylanicum was able to give 2.3-fold increase in phycocyanin production in comparison to commonly used BG 11 medium in 32 days. We have demonstrated the extraction, purification and characterization of C-phycocyanin using novel method based on filtration and single step chromatography. The protein was extracted by repeated freeze-thaw cycles and the crude extract was filtered and concentrated in stirred ultrafiltration cell (UFC). The UFC concentrate was then subjected to a single ion exchange chromatographic step. A purity ratio of 4.15 was achieved from a starting value of 1.05. The recovery efficiency of C-phycocyanin from crude extract was 63.50%. The purity was checked by electrophoresis and UV-Vis spectroscopy.
authors.affiliation: BRD School of Biosciences, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388120, Gujarat, India.
authors.forename: Niraj Kumar
authors.initials: NK
authors.lastname: Singh
authors.forename: Asha
authors.initials: A
authors.lastname: Parmar
authors.forename: Datta
authors.initials: D
authors.lastname: Madamwar
mesh_terms: D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D002852:Chromatography, Ion Exchange; D045424:Complex Mixtures; D003470:Culture Media; D000458:Cyanobacteria / Q000254:growth & development / Q000378:metabolism*; D004586:Electrophoresis; D052979:Phycobiliproteins / Q000378:metabolism; D010798:Phycocyanin / Q000096:biosynthesis* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling; D014462:Ultrafiltration; D019345:Zinc Acetate
chemical_list: D045424:Complex Mixtures; D003470:Culture Media; D052979:Phycobiliproteins; D010798:Phycocyanin; D019345:Zinc Acetate