Bacterial product cantastim derived from Pseudomonas aeruginosa induces migration and maturation of dendritic cells.
Dendritic cells (DCs) play a pivotal role in linking innate and adaptive immunity. Migration to the lymph nodes and maturation of DCs are crucial steps in the initiation of specific immune responses. The bacterial product CANTASTIM (CS) is a purified extract of Pseudomonas aeruginosa that induces non-specific protection against bacterial infection, enhances macrophage effector functions and modulates cytokines production. In this study, we used a mouse skin explant culture model and human monocyte-derived DCs to study the effect of CS on the migration and maturation of DCs, respectively. We noticed a significant increase in the number of DCs which migrated from the skin explants when CS was added to the culture medium. Also, CS was able to induce the expression of maturation-associated marker CD83 on human monocyte-derived DCs. DC-based tumor vaccines represent a promising approach for cancer immunotherapy and the migration rate and maturation state of DCs are important parameters for their clinical effectiveness. CS may be an attractive candidate to be tested for the production of DC-based vaccine.
Caraş
Iuliana
I
Infection and Immunity Laboratory, Cantacuzino National Institute of Research and Development for Microbiology and Immunology, Bucharest, Romania. immuno@cantacuzino.ro
Tucureanu
Cătălin
C
Lerescu
Lucian
L
Sălăgeanu
Aurora
A
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D010743:Phospholipids / Q000378:metabolism / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012867:Skin / Q000166:cytology
D003470:Culture Media; D010743:Phospholipids; C049675:cantastim