Circular dichroism study of enzymatic manipulation on magnetic and metallic DNA template nanowires.
Circular dichroism spectroscopy (CD) was used to examine the mechanism of endonuclease clipping and ligation of the DNA template nanowires. The biomolecular manipulation of the DNA template is compared for both metallic (Au) and magnetic (Fe(2)O(3) and CoFe(2)O(4)) nanowires. The dependence of nanoparticle (NP) concentration on enzymatic clipping and DNA ligation was studied, in addition to performing absorbance and thermal melting experiments. Low-NP concentration preserved and digested the DNA template structure. Yet, at higher NP concentrations, the DNA template began to denature before enzyme addition. It was also observed that ligation of the digested DNA occurred more efficiently at low-NP concentrations. These results provide significant information on structural alteration and biorecognition effectiveness of the DNA template after enzymatic manipulation.
Jaganathan
Hamsa
H
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47906, USA.
Ivanisevic
Albena
A
D002942:Circular Dichroism / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*
D004247:DNA; D004262:DNA Restriction Enzymes