title: An in vivo evaluation of the biocompatibility of anodic plasma chemical (APC) treatment of titanium with calcium phosphate.
abstract: Implant loosening is an unresolved complication associated with prosthetics. Previous studies report improved osseointegration with hydroxyapatite (HA) or tri-calcium phosphate coatings. Unfortunately, the brittleness and low strength of these coatings in adhesion to the implant or internal cohesion is problematic, restricting their use. Anodic plasma-chemical (APC) treatment, an advanced anodisation method, allows for porous oxide layer formation with incorporation of calcium and phosphate directly into the oxide. This produces superior adhesive strength than a conventional coating of calcium phosphate offering potential for long-term osseointegration. Although the cytocompatibility of several APC treatments have been previously shown, this study was the first to investigate the biocompatibility and osteoconductivity of APC surfaces in vivo when compared with standard HA coated and noncoated commercially pure titanium implant cortical screws. Sample screws were implanted in female Swiss alpine sheep for 12 weeks. Bone remodelling in situ, differences in bone apposition resulting in cortical thickening as well as peak removal torque measurements were assessed. We found no significant differences between the tested coatings and no delamination was observed with any of the APC-treated surfaces. The results suggest that APC-treated samples have similar biological performance to HA-coated screws. In our opinion, APC treatment, which also has superior binding strength to the base metal compared with standard HA coatings as well as similar biocompatibility as shown here, holds great potential for biomedical applications. Now that the in vivo biocompatibility has been proven, the work is being extended to more challenging in vivo models.
authors.affiliation: AO Research Institute, AO Foundation, Davos, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Schlegel
authors.forename: J S
authors.initials: JS
authors.lastname: Hayes
authors.forename: V M
authors.initials: VM
authors.lastname: Frauchiger
authors.forename: B
authors.initials: B
authors.lastname: Gasser
authors.forename: R
authors.initials: R
authors.lastname: Wieling
authors.forename: M
authors.initials: M
authors.lastname: Textor
authors.forename: R G
authors.initials: RG
authors.lastname: Richards
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002130:Calcium Phosphates / Q000737:chemistry*; D004566:Electrodes; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D012756:Sheep; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D005456:Fluorescent Dyes; C020243:calcium phosphate; D014025:Titanium