Analysis of methylated circulating DNA in cancer patients' blood.
Circulating extracellular nucleic acids derived from body fluids such as blood are commonly analyzed to assess malignant diseases. Efficient isolation, extraction, quantification, modification, and analysis methods remain important for utilizing circulating nucleic acids as potential molecular biomarkers. Our refined techniques of DNA isolation from serum, sodium bisulfite modification of extracted DNA, and methylation analysis provide a robust approach for quantitative analysis of circulating tumor-related DNA. The approach allows direct comparison of methylated and nonmethylated genomic sequences in a specimen.
Sunami
Eiji
E
Department of Molecular Oncology, John Wayne Cancer Institute and Breast Center, Saint John's Health Center, Santa Monica, CA, USA.
Vu
Anh-Thu
AT
Nguyen
Sandy L
SL
Hoon
Dave S B
DS
D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000097:blood* / Q000737:chemistry* / Q000235:genetics; D019075:Electrophoresis, Capillary; D006801:Humans; D009369:Neoplasms / Q000097:blood* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D013447:Sulfites
D004273:DNA, Neoplasm; D013447:Sulfites; C009279:sodium bisulfite