LebZIP2 induced by salt and drought stress and transient overexpression by Agrobacterium.
The full-length cDNA of LebZIP2 (Lycopersicon esculentum bZIP2) encodes a protein of 164 amino acids and contains a N-terminal basic-region leucine zipper domain. Analysis of the deduced tomato LebZIP2 amino acid sequence revealed that it shares 85% sequence identity with both tobacco bZIP and pepper CcbZIP. LebZIP2 mRNA is expressed at a high level exclusively in flowers. Presently, LebZIP2 was strongly increased also following NaCl and mannitol treatments. No significant LebZIP2 expression was evident following cold treatment. Transient LebZIP2 overexpression resulted in increased NbNOA1 and NbNR transcript levels in Nicotiana benthamiana leaves. Our results indicate that LebZIP2 might play roles as an abiotic stress-signaling pathway and as a transcriptional regulator of the NbNOA1 or NbNR genes.
Seong
Eun Soo
ES
Bioherb Research Institute, College of Agriculture and life Science, Kangwon National University, Chuncheon, Korea.
Kwon
Soon Sung
SS
Ghimire
Bimal Kumar
BK
Yu
Chang Yeon
CY
Cho
Dong Ha
DH
Lim
Jung Dae
JD
Kim
Kyoung Su
KS
Heo
Kweon
K
Lim
Eun-Sang
ES
Chung
Ill-Min
IM
Kim
Myong Jo
MJ
Lee
Youn Su
YS
D000595:Amino Acid Sequence; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005822:Genetic Vectors; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics*; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D009997:Osmotic Pressure / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics*; D020539:Sequence Analysis, Protein; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects
D010940:Plant Proteins; D008353:Mannitol; D012965:Sodium Chloride