Alternative requirements for Vestigial, Scalloped, and Dmef2 during muscle differentiation in Drosophila melanogaster.
Vertebrate development requires the activity of the myocyte enhancer factor 2 (mef2) gene family for muscle cell specification and subsequent differentiation. Additionally, several muscle-specific functions of MEF2 family proteins require binding additional cofactors including members of the Transcription Enhancing Factor-1 (TEF-1) and Vestigial-like protein families. In Drosophila there is a single mef2 (Dmef2) gene as well single homologues of TEF-1 and vestigial-like, scalloped (sd), and vestigial (vg), respectively. To clarify the role(s) of these factors, we examined the requirements for Vg and Sd during Drosophila muscle specification. We found that both are required for muscle differentiation as loss of sd or vg leads to a reproducible loss of a subset of either cardiac or somatic muscle cells in developing embryos. This muscle requirement for Sd or Vg is cell specific, as ubiquitous overexpression of either or both of these proteins in muscle cells has a deleterious effect on muscle differentiation. Finally, using both in vitro and in vivo binding assays, we determined that Sd, Vg, and Dmef2 can interact directly. Thus, the muscle-specific phenotypes we have associated with Vg or Sd may be a consequence of alternative binding of Vg and/or Sd to Dmef2 forming alternative protein complexes that modify Dmef2 activity.
Deng
Hua
H
Department of Cell Biology, Department of Biological Sciences, and Department of Medical Genetics, University of Alberta, Edmonton, Canada.
Hughes
Sarah C
SC
Bell
John B
JB
Simmonds
Andrew J
AJ
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000196:embryology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009024:Morphogenesis / Q000502:physiology; D046912:Multiprotein Complexes / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000502:physiology*; D009154:Mutation; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D018006:Myogenic Regulatory Factors / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D029721:Drosophila Proteins; C499409:Mef2 protein, Drosophila; D046912:Multiprotein Complexes; D018006:Myogenic Regulatory Factors; D009687:Nuclear Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C087984:sd protein, Drosophila; C102933:tin protein, Drosophila; C095064:vg protein, Drosophila