Electrochemical determination of nitric oxide and of its derivatives.
Nitric oxide (NO) is one of the simplest odd electron species. Furthermore, it is relatively hydrophobic, which is consistent with its role as a diffusible intracellular messenger or as an immune effector. NO is generated in biological systems and plays important roles as a regulatory molecule. The main problem in NO analysis is its extreme reactivity; in aerated water solution it is transformed into nitrite and nitrate, inactive biological forms. Moreover, it may lose an electron forming the NO(+) ion, involved in the synthesis of nitrosothiols (RSNOs). The main problems encountered in the analytical determination of free NO and of RSNOs in biological systems are the low stability and the very low concentration of these compounds. The determination of nitrates and nitrites may also be difficult when their concentration is in the nmolar range. We describe an electrochemical assay for the determination in the same sample of free NO and of its derivatives in nmolar range. Owing to its high sensitivity, the procedure could also be applied to environmental analyses.
Palmerini
Carlo A
CA
Dipartimento di Scienze Biochimiche Biotecnologie Molecolari, Università di Perugia, Via del Giochetto, 06126 Perugia, Italy.
Arienti
Giuseppe
G
Palombari
Roberto
R