Association of p53 codon 72 polymorphism with liver metastases of colorectal cancers positive for p53 overexpression.
OBJECTIVE
To evaluate the association between p53 codon 72 polymorphism (R72P) and the risk of colorectal liver metastases.


METHODS
The p53 R72P genotype was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 78 consecutive colorectal cancer patients with liver metastases and 214 age- and sex-matched cases with nonmetastatic colorectal cancer.


RESULTS
The R allele of the p53 R72P polymorphism was more frequently found in metastatic cases than in nonmetastatic cases (P=0.075). Carriers of the 72R allele had a 2.25-fold (95% CI (confidence interval)=1.05 to approximately 4.83) increased risk of liver metastases. On the stratification analysis, 72R-carrying genotype conferred a 3.46-fold (95% CI=1.02 to approximately 11.72) and a 1.05-fold (95% CI=0.36 to approximately 3.08) increased risk of liver metastases for p53 overexpression-positive and negative colorectal cancers, respectively.


CONCLUSIONS
These results demonstrate for the first time that the 72R allele of the p53 polymorphism has an increased risk for liver metastases in colorectal cancers positive for p53 overexpression.
Zhu
Zhong-Zheng
ZZ
Department of Pathology, No. 113 Hospital of People's Liberation Army, Ningbo 315040, China. zzzhu1170@yahoo.com
Liu
Bing
B
Wang
Ai-Zhong
AZ
Jia
Hang-Ruo
HR
Jin
Xia-Xiang
XX
He
Xiang-Lei
XL
Hou
Li-Fang
LF
Zhu
Guan-Shan
GS
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000556:secondary*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics; D005260:Female; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000556:secondary*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics
D004273:DNA, Neoplasm; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53