Method for the simultaneous determination of total polyphenol and anthocyan indexes in red wines using a flow injection approach.
A simultaneous and fast method for the determination of total polyphenol index (t.p.i.) and total anthocyan index (t.a.i.) has been developed by a flow injection approach and a diode array spectrophotometer for monitoring at 280 and 520 nm, respectively. Linear ranges were obtained from 20 to 70 index units and from 20 to 500 mg l(-1) for the t.p.i. and t.a.i., respectively. The results provided by the proposed method agree with those obtained using the polyphenol index at 280 nm and the Ribereau-Gayon method for the determination of total anthocyans. The sample throughout was 25-30 samples per hour. Analytical features such as repeatability, reproducibility and detection and quantification limits as well as the results of a robustness study based on the Steiner-Younden procedure are also given.
González-Rodríguez
J
J
R&D Department Pérez Barquero, S.A. Avda de Andalucia, 27, Montilla, E-14550 Córdoba, Spain.
Pérez-Juan
P
P
Luque de Castro
M D
MD