title: Stable isotope ratio analysis as a tool to discriminate between rainbow trout (O. mykiss) fed diets based on plant or fish-meal proteins.
abstract: The use of stable isotope ratio analysis (SIRA) as a rapid analytical tool to characterize and discriminate farmed fish on the basis of the feedstuffs included in the diet formulation is discussed. Two isoproteic (44.8%) and isolipidic (19.6%) extruded diets were formulated: a fish-meal-based diet (FM diet), containing fish meal as the sole protein source; a plant-protein-based diet (PP diet), where pea protein concentrate and wheat gluten meal replaced 80% of fish meal protein. The diets were fed to eight groups of rainbow trout (initial body weight: 106.6g) for 103 days in two daily meals under controlled rearing conditions. Growth performance (final body weight: 318.5 g; specific growth rate: 1.06%) and feed-to-gain ratio (0.79) were not affected by the dietary treatment. The differences in isotopic values of the two diets were clearly reflected in the different carbon and nitrogen isotopic values in rainbow trout fillets. The delta(13)C and delta(15)N values of muscle of farmed rainbow trout showed differences between farmed fish fed a fish-protein-based diet (-20.47 +/- 0.34 and 12.38 +/- 0.57 for delta(13)C and delta(15)N, respectively) and those fed a plant-protein-based diet (-23.96 +/- 0.38 and 7.15 +/- 0.51 for delta(13)C and delta(15)N, respectively). The results suggest that SIRA provides a robust and verifiable analytical tool to discriminate between fish fed on a plant or a fish protein diet.
authors.affiliation: Institute for Health and Consumer Protection, Physical and Chemical Exposure Unit, MAST-Group, TP281, Via Fermi 2, 21020 Ispra (VA), Italy. jose-manuel.moreno-rojas@jrc.it
authors.forename: J M
authors.initials: JM
authors.lastname: Moreno-Rojas
authors.forename: F
authors.initials: F
authors.lastname: Tulli
authors.forename: M
authors.initials: M
authors.lastname: Messina
authors.forename: E
authors.initials: E
authors.lastname: Tibaldi
authors.forename: C
authors.initials: C
authors.lastname: Guillou
mesh_terms: D000821:Animal Feed; D000818:Animals; D002247:Carbon Isotopes / Q000032:analysis*; D004032:Diet; D004044:Dietary Proteins / Q000032:analysis; D005396:Fish Products / Q000032:analysis; D029941:Fish Proteins / Q000008:administration & dosage* / Q000737:chemistry; D005983:Glutens / Q000737:chemistry; D013058:Mass Spectrometry; D009587:Nitrogen Isotopes / Q000032:analysis*; D017686:Oncorhynchus mykiss / Q000254:growth & development* / Q000378:metabolism; D018532:Pisum sativum / Q000737:chemistry; D010940:Plant Proteins / Q000008:administration & dosage* / Q000737:chemistry; D014908:Triticum / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D004044:Dietary Proteins; D029941:Fish Proteins; D009587:Nitrogen Isotopes; D010940:Plant Proteins; D005983:Glutens