Spatiotemporal regulation of fibroblast growth factor signal blocking for endoderm formation in Xenopus laevis.
We have previously shown that the inhibition of fibroblast growth factor (FGF) signaling induced endodermal gene expression in the animal cap and caused the expansion of the endodermal mass in Xenopus embryos. However, we still do not know whether or not the alteration of FGF signaling controls embryonic cell fate, or when FGF signal blocking is required for endoderm formation in Xenopus. Here, we show that FGF signal blocking in embryonic cells causes their descendants to move into the endodermal region and to express endodermal genes. It is also interesting that blocking FGF signaling between fertilization and embryonic stage 10.5 promotes endoderm formation, but persistent FGF signaling blocking after stage 10.5 restricts endoderm formation and differentiation.
Cha
Sang-wook
SW
Department of Anatomy, School of Medicine, Kyungpook National University, Daegu 700-412, Korea.
Lee
Jong-Woo
JW
Hwang
Yoo-seok
YS
Chae
Jeong-Pil
JP
Park
Kwon Moo
KM
Cho
Hee Jung
HJ
Kim
Dong Sun
DS
Bae
Yong Chul
YC
Park
Mae Ja
MJ
D000818:Animals; D004707:Endoderm / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D017403:In Situ Hybridization; D011758:Pyrroles / Q000008:administration & dosage / Q000494:pharmacology; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D029867:Xenopus Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics / Q000502:physiology*
D011758:Pyrroles; D017468:Receptors, Fibroblast Growth Factor; C105686:SU 5402; D029867:Xenopus Proteins; D005346:Fibroblast Growth Factors