Translation initiation factor 4B homodimerization, RNA binding, and interaction with Poly(A)-binding protein are enhanced by zinc.
The eukaryotic translation initiation factor (eIF) 4B promotes the RNA-dependent ATP hydrolysis activity and ATP-dependent RNA helicase activity of eIF4A and eIF4F during translation initiation. eIF4B also helps to organize the assembly of the translational machinery through its interactions with eIF4A, eIF4G, eIF3, the poly(A)-binding protein (PABP), and RNA. Although the function of eIF4B is conserved among plants, animals, and yeast, eIF4B is one of the least conserved of initiation factors at the sequence level. Mammalian eIF4B is a constitutive dimer; however, conflicting reports have suggested that plant eIF4B may exist as a monomer or a dimer. In this study, we show that eIF4B from wheat can form a dimer and we identify the region responsible for its dimerization. Zinc stimulated homodimerization of eIF4B and bound eIF4B with a Kd of 19.7 nM. Zinc increased the activity of the eIF4B C-terminal RNA-binding domain specifically. Zinc promoted the interaction between eIF4B and PABP but not the interaction between eIF4B and eIF4A or eIFiso4G, demonstrating that the effect of zinc was highly specific. The interaction between PABP and eIFiso4G was also stimulated by zinc but required significantly higher levels of zinc. Interestingly zinc abolished the ability of eIFiso4G to compete with eIF4B in binding to their overlapping binding sites in PABP by preferentially promoting the interaction between eIF4B and PABP. Our observations suggest that wheat eIF4B can dimerize but requires zinc. Moreover zinc controls the partner protein selection of PABP such that the interaction with eIF4B is preferred over eIFiso4G.
Cheng
Shijun
S
Department of Biochemistry, University of California, Riverside, California 92521-0129, USA.
Sultana
Shemaila
S
Goss
Dixie J
DJ
Gallie
Daniel R
DR
D000595:Amino Acid Sequence; D001665:Binding Sites; D002712:Chlorides / Q000494:pharmacology; D019281:Dimerization; D039642:Eukaryotic Initiation Factors / Q000737:chemistry*; D005982:Glutathione Transferase / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D039102:Poly(A)-Binding Proteins / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000378:metabolism; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology*; D017967:Zinc Compounds / Q000494:pharmacology
D002712:Chlorides; D039642:Eukaryotic Initiation Factors; D039102:Poly(A)-Binding Proteins; D017967:Zinc Compounds; C031799:eIF-4B; D012313:RNA; C016837:zinc chloride; D005982:Glutathione Transferase; D015032:Zinc