title: Improved sample injection and illumination for multicapillary systems.
abstract: Multicapillary electrophoresis continues to see improvements in speed, robustness, and reliability. This paper reports on our work on two components belonging to a multicapillary sequencer developed in our group. Injection of the DNA samples into the capillaries was optimized to make it reproducible and to reduce the amount of sample volume required. An alternative laser illumination of the capillaries was also developed. Light intensity in the capillaries was increased as a result of a step-by-step scanning of the laser and the use of microlenses in front of the capillaries.
authors.affiliation: Centre National de Sciences et Technologies Nucléaires, Sidi-Thabet, Tunisia. nidhal.kahlaoui@cnstn.rnrt.tn
authors.forename: Nidhal
authors.initials: N
authors.lastname: Kahlaoui
authors.forename: Marie-Alix
authors.initials: MA
authors.lastname: Duval
authors.forename: Françoise
authors.initials: F
authors.lastname: Lefebvre
authors.forename: Yves
authors.initials: Y
authors.lastname: Charon
authors.forename: Rainer
authors.initials: R
authors.lastname: Siebert
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D007834:Lasers