Phylogeny of G9 rotavirus genotype: a possible explanation of its origin and evolution.
BACKGROUND
G9 rotavirus genotype was isolated in the 1980s and re-emerged without a clear explanation in the mid-1990s as one of the most frequently occurring genotypes with distinct genetic and molecular characteristics.


OBJECTIVE
To study the G9 genotype sequence polymorphisms in Spain and compare them with the human and porcine G9 VP7 genes from the rest of the world. Complete phylogenetic analyses have been done to better characterize G9 genotypes, their relationships and evolution.


METHODS
Twelve G9 VP7 genes from Spanish patients were sequenced and compared with 240 G genotype sequences. Nucleotide and amino acid sequence similarity percentages and neighbour-joining dendrograms were used to establish a new phylogenetic analysis.


RESULTS
Eight of the 12 Spanish sequenced samples had different nucleotide translated region sequences, which yielded only five different proteins. New nucleotide and amino acid sequence comparisons were made that differed from previously described results.


CONCLUSIONS
Spanish G9 genotype sequences have similar structure of those belonging to lineage III as the majority of the G9 sequences and share amino acid motifs with other sequences. The phylogenetic analyses of G9 genotypes confirmed the existence of 6 lineages, but did not confirm the 11 sublineages previously reported.
Martinez-Laso
Jorge
J
Unidad de Inmunoterapia Celular, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km 2.2, 28220 Majadahonda, Madrid, Spain. jmlaso@isciii.es
Román
Angela
A
Head
Jacqueline
J
Cervera
Isabel
I
Rodríguez
Miriam
M
Rodríguez-Avial
Iciar
I
Picazo
Juan J
JJ
D000818:Animals; D016000:Cluster Analysis; D019143:Evolution, Molecular; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013030:Spain; D013553:Swine Diseases / Q000821:virology
D012367:RNA, Viral