title: A practical synthesis of the 16C/15N-labelled tripeptide N-formyl-Met-Leu-Phe, useful as a reference in solid-state NMR spectroscopy.
abstract: A mild synthetic method for N-formyl-Met-Leu-Phe-OH (1) is described. After Fmoc solid phase peptide synthesis, on-bead formylation and HPLC purification, more than 30 mg of the fully (13)C/(15)N-labelled tripeptide 1 could be isolated in a typical batch. This peptide can be easily crystallised and is therefore well suited as a standard sample for setting up solid-state NMR experiments.
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University Frankfurt, Max-von-Laue-Str. 7, D-60438 Frankfurt am Main, Germany.
authors.forename: Sven T
authors.initials: ST
authors.lastname: Breitung
authors.affiliation: Institute of Biophysical Chemistry, Johann Wolfgang Goethe University Frankfurt, Max-von-Laue-Str. 9, D-60438 Frankfurt am Main, Germany.
authors.forename: Jakob J
authors.initials: JJ
authors.lastname: Lopez
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University Frankfurt, Max-von-Laue-Str. 7, D-60438 Frankfurt am Main, Germany.
authors.forename: Gerd
authors.initials: G
authors.lastname: Dürner
authors.affiliation: Institute of Biophysical Chemistry, Johann Wolfgang Goethe University Frankfurt, Max-von-Laue-Str. 9, D-60438 Frankfurt am Main, Germany.
authors.forename: Clemens
authors.initials: C
authors.lastname: Glaubitz
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University Frankfurt, Max-von-Laue-Str. 7, D-60438 Frankfurt am Main, Germany.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Göbel
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University Frankfurt, Max-von-Laue-Str. 7, D-60438 Frankfurt am Main, Germany.
authors.forename: Marcel
authors.initials: M
authors.lastname: Suhartono