Measuring estrogens using flow injection immunoanalysis with liposome amplification.
A solid-phase competitive immunoassay is performed in flow injection analysis for the measurement of the hormone 17-beta-estradiol. The flow injection analysis system incorporates a column-type reactor packed with solid silica particles onto which we have covalently immobilized the antigen 17-beta-estradiol. Anti-estradiol is noncovalently conjugated to the liposome through a streptavidin-biotin linkage. When mixed with a sample containing the antigen, the antibody binding sites on the liposomes are complexed which reduces the binding of liposomes to the solid support in a concentration-dependent manner. Sequential immunoassays are performed on-column following a simple regeneration step.
Locascio-Brown
L
L
National Institute of Standards and Technology, Gaithersburg MD 20899 U.S.A.
Choquette
S J
SJ