Advancing multifocal nonlinear microscopy: development and application of a novel multibeam Yb:KGd(WO4)2 oscillator.
We present a novel Yb:KGd(WO(4))(2) oscillator design that generates six beams of temporally delayed, 253 fs, 11 nJ pulses. This allows multifocal nonlinear microscopy to be performed without the need for complicated optical multiplexers. We demonstrate our design with twelve simultaneously acquired two-photon, second-harmonic and/or third-harmonic images generated from six laterally separated foci.
Sheetz
Kraig E
KE
Department of Physics, Colorado School of Mines, Golden, Colorado 80401, USA. ksheetz@mines.edu
Hoover
Erich E
EE
Carriles
Ramón
R
Kleinfeld
David
D
Squier
Jeff A
JA