Room-temperature phosphorimetry studies of some addictive drugs following dansyl chloride labelling.
A room-temperature phosphorimetric (RTP) method for the analysis of barbital, codeine, morphine and practolol after labelling with dansyl chloride (DNS-Cl) is described. The drug-DNS derivatives were obtained by refluxing with drug-ethyl acetate solutions and solid DNS-Cl in the presence of anhydrous potassium carbonate. The reaction conditions were investigated in detail. The fluorescence emission of drug-DNS derivatives shifted to longer wavelengths compared with that of DNS-Cl. The RTP phenomena observed for these derivatives by using a micellar stabilized room-temperature phosphorescence technique were examined and optimum conditions for their RTP emission were studied using an orthogonal array design. Derivative RTP spectra were obtained and successfully used to determine practolol by the established method without further separation.
Tong
A J
AJ
Wu
Y G
YG
Li
L D
LD