title: Chemiluminescence determination of sodium 2-mercaptoethane sulfonate by flow injection analysis using cerium(IV) sensitized by quinine.
abstract: A flow injection method with chemiluminescence (CL) detection is proposed for the determination of sodium 2-mercaptoethane sulfonate (MESNA), a drug often used to reduce the urotoxic effects of antineoplastic alkylating agents. The procedure is based on the reaction of the thiol with Ce(IV) in a sulfuric acid medium and measurement of the CL intensity produced by quinine used as a sensitizer. The optimum conditions for CL emission were investigated. Using the CL peak height as the analytical parameter, MESNA was determined over the concentration range 0.29-2.21 ng (1.97-9.85 mug.l(-1)) with a detection limit of 0.21 ng (1.38 mug.l(-1)) and a relative standard deviation (R.S.D.) of 4.1%. The method was applied satisfactorily to the determination of MESNA in pharmaceutical preparations with percentages of recovery between 94 and 105.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, Spain.
authors.forename: L F
authors.initials: LF
authors.lastname: Capitán-Vallvey
authors.forename: M C
authors.initials: MC
authors.lastname: Valencia Mirón
authors.forename: R
authors.initials: R
authors.lastname: Acosta Acosta