title: Reanalysis of the testosterone displacement from its HSA binding site by DHEA using competitive Langmuir isotherm.
abstract: In a previous manuscript [C. Andre, A. Berthelot, J.F. Robert, M. Thomassin, Y.C. Guillaume, J. Pharm. Bio. Med., in press], the Mg(2+) effect on the testosterone displacement equilibrium from its human serum albumin (HSA) binding site by DHEA was investigated using a thermodynamic approach. In this paper, a novel concept based on the competitive Langmuir distribution isotherms was proposed to calculate the association constant of the HSA-hormone binding and to confirm the Mg(2+) role on the testosterone displacement equilibrium from its HSA binding site by DHEA. Thus, both the HSA-hormone binding and the displacement equilibrium processes were reanalysed. The results obtained confirmed that:
authors.affiliation: Equipe des Sciences Séparatives et Biopharmaceutiques (2SB), Laboratoires de Chimie Analytique, Faculté de Médecine et de Pharmacie, Place Saint-Jacques, 25030 Besançon cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: André
authors.forename: Alain
authors.initials: A
authors.lastname: Xicluna
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Robert
authors.forename: Mireille
authors.initials: M
authors.lastname: Thomassin
authors.forename: Yves-Claude
authors.initials: YC
authors.lastname: Guillaume