Detection of P. aeruginosa using nano-structured electrode-separated piezoelectric DNA biosensor.
A DNA biosensor for detection of Pseudomonas aeruginosa was set up based on the modification of two membranes (nano-TiO(2) and nano-TiO(2)-polyethylene glycol hybrid membrane) to the ESPS surface. These two membrane materials were synthesized by sol-gel method. The detection was accomplished by modifying ss-DNA on the sensitive membrane and then hybridizing with their complementary strands from the P. aeruginosa in liquid phase. UV spectrum was used to identify the purity and concentration of extracted DNA; IR spectrum and SEM were used to characterize the properties of the membrane. The detection was highly improved by adoption of nanotechnology and hybrid membrane. Less than 3h was sufficient. The detection linear range was from 10(-1) to 10(-3)gl(-1) and the limit of detection was 10(-4)gl(-1).
He
Fengjiao
F
College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Liu
Suqin
S