title: Enhanced systemic matrix metalloproteinase response in Helicobacter pylori gastritis.
abstract: BACKGROUND
  Helicobacter pylori causes chronic gastritis, peptic ulcer disease, and is the most important risk factor for non-cardia gastric cancer, and has been shown to upregulate matrix metalloproteinases (MMPs) in infected gastric mucosa. MMPs are proteolytic enzymes regulated by tissue inhibitors of metalloproteinases (TIMPs).
  
  
  OBJECTIVE
  We set up this study to find out whether H. pylori gastritis induces systemic MMP response.
  
  
  METHODS
  Serum samples were collected from patients undergoing gastroscopy; 26 patients had H. pylori gastritis and 18 were H. pylori-negative controls with normal gastric mucosa. Serum MMP levels were analysed by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Significantly elevated serum levels of collagenase-2 (MMP-8), gelatinase B (MMP-9), neutrophil elastase (NE), and myeloperoxidase (MPO), and reduced serum levels of gelatinase A (MMP-2) and TIMP-1 were demonstrated in patients with H. pylori gastritis as compared to H. pylori-negative controls. No significant differences were shown in serum matrilysin-1 (MMP-7) levels.
  
  
  CONCLUSIONS
  For the first time, we show enhanced MMP-8 response in H. pylori infection together with other neutrophil degranulation products (MMP-9, MPO, NE). Elevated circulating neutrophil degranulation product levels in serum of H. pylori-positive patients reflect accelerated proteolysis and oxidative stress, and may contribute to extraintestinal sequelae, such as cardiovascular diseases.
authors.affiliation: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki and HUSLAB, Helsinki University Central Hospital Laboratory, Finland. hilpi.rautelin@helsinki.fi
authors.forename: Hilpi I
authors.initials: HI
authors.lastname: Rautelin
authors.forename: Aino M
authors.initials: AM
authors.lastname: Oksanen
authors.forename: Lea I
authors.initials: LI
authors.lastname: Veijola
authors.forename: Pentti I
authors.initials: PI
authors.lastname: Sipponen
authors.forename: Taina I
authors.initials: TI
authors.lastname: Tervahartiala
authors.forename: Timo A
authors.initials: TA
authors.lastname: Sorsa
authors.forename: Anneli
authors.initials: A
authors.lastname: Lauhio
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005756:Gastritis / Q000201:enzymology* / Q000382:microbiology; D016481:Helicobacter Infections / Q000201:enzymology* / Q000382:microbiology; D016480:Helicobacter pylori; D006801:Humans; D019272:Leukocyte Elastase / Q000097:blood; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000097:blood; D020784:Matrix Metalloproteinase 8 / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D020782:Matrix Metalloproteinases / Q000097:blood*; D053505:Matrix Metalloproteinases, Secreted / Q000097:blood; D008875:Middle Aged; D009195:Peroxidase / Q000097:blood; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood; D015854:Up-Regulation
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D009195:Peroxidase; D019272:Leukocyte Elastase; D020782:Matrix Metalloproteinases; D053505:Matrix Metalloproteinases, Secreted; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9