The signal vs. concentration relationships in fluorimetry.
Formulae for the fluorescence signal are derived which take into account some factors hitherto neglected. From these formulae conditions are deduced, for which a linear relationship exists between the concentration of the fluorescent components and the intensity of the fluorescence. In some cases of practical interest the fulfilment of these conditions cause too low a sensitivity. For such cases a method of optimization is outlined, by means of which the conditions are found which lead to the least deviation from linearity at the required sensitivity.
van Slageren
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Laboratory for Analytical Chemistry, University of Amsterdam, Nieuwe Achtergracht 125, Amsterdam, The Netherlands.
den Boef
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van der Linden
W E
WE