The cytotoxic effect of phenol and ethanol on the chondrosarcoma-derived cell line OUMS-27: an in vitro experiment.
Surgery is considered to be the most effective treatment for cartilaginous tumours. In recent years, a trend has emerged for patients with low-grade tumours to be treated less invasively using curettage followed by various forms of adjuvant therapy. We investigated the potential for phenol to be used as an adjuvant. Using a human chondrosarcoma-derived cartilage-producing cell line OUMS-27 as an in vitro model we studied the cytotoxic effect of phenol and ethanol. Since ethanol is the standard substance used to rinse phenol out of a bone cavity, we included an assessment of ethanol to see whether this was an important secondary factor with respect to cell death. The latter was assessed by flow cytometry. A cytotoxic effect was found for concentrations of phenol of 1.5% and of ethanol of 42.5%. These results may provide a clinical rationale for the use of both phenol and ethanol as adjuvant therapy after intralesional curettage in low-grade central chondrosarcoma and justify further investigation.
Verdegaal
S H M
SH
Department of Orthopaedics, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands. shmverdegaal@hotmail.com
Corver
W E
WE
Hogendoorn
P C W
PC
Taminiau
A H M
AH
D002460:Cell Line; D017024:Chemotherapy, Adjuvant; D002813:Chondrosarcoma / Q000188:drug therapy*; D003475:Curettage; D003603:Cytotoxins / Q000737:chemistry / Q000627:therapeutic use*; D000431:Ethanol / Q000737:chemistry / Q000627:therapeutic use*; D006801:Humans; D019800:Phenol / Q000737:chemistry / Q000627:therapeutic use*; D013223:Statistics as Topic
D003603:Cytotoxins; D019800:Phenol; D000431:Ethanol