title: Label-free detection of cancer biomarker candidates using surface plasmon resonance imaging.
abstract: In this work, we present an antibody array for the detection of cancer biomarker candidates by a surface plasmon resonance (SPR) imaging sensor with polarization contrast. Responses from the SPR imaging sensor are shown to be similar to those from a conventional spectroscopy-based SPR sensor. Antibodies are spotted onto a self-assembled monolayer (SAM) composed of oligo(ethylene glycol) (OEG)-containing alkanethiol chains. Detection of two cancer biomarker candidates, activated leukocyte cell adhesion molecule/CD 166 (ALCAM) and transgelin-2 (TAGLN2), is demonstrated. Limits of detection for ALCAM and TAGLN2 are established at 6 ng/mL and 3 ng/mL, respectively, in buffer. No cross-reactivity is observed between immobilized antibodies and nonspecific antigen. Biomarker candidates are also detected in a 10% human serum solution.
authors.affiliation: Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Jon
authors.initials: J
authors.lastname: Ladd
authors.forename: Allen D
authors.initials: AD
authors.lastname: Taylor
authors.forename: Marek
authors.initials: M
authors.lastname: Piliarik
authors.forename: Jiri
authors.initials: J
authors.lastname: Homola
authors.forename: Shaoyi
authors.initials: S
authors.lastname: Jiang
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D002138:Calibration; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor