title: Two-stage culture for producing berberine by cell suspension and shoot cultures of Berberis buxifolia Lam.
abstract: In vitro cultures of Berberis buxifolia were established using thidiazuron (4.5, 23 and 45 mM) or picloram (4 and 40 mM) as plant growth regulators for sustaining growth. For producing berberine, a two-stage culture was performed. In the first step, thidiazuron or picloram were used for biomass production followed by the production stage where benzylaminopurine (4.4 mM) was added as a plant growth regulator. Berberine yields (102 mg g(-1) DW) and in vitro shoot cultures (200 mg g(-1) DW) were significantly lower than those of whole plants in the field (416 mg g(-1) DW). The highest productivity (0.18 mg 1(-1) day(-1)) was attained using picloram (either 4 on 40 mM) in the first stage for producing biomass.
authors.affiliation: Instituto de Ciencia y Tecnología Dr César Milstein, CONICET, Saladillo, 2468 (C1440FFX), Ciudad de Buenos Aires, Argentina.
authors.forename: María A
authors.initials: MA
authors.lastname: Alvarez
authors.forename: Natalia Fernandez
authors.initials: NF
authors.lastname: Eraso
authors.forename: Sandra I
authors.initials: SI
authors.lastname: Pitta-Alvarez
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Marconi
mesh_terms: D001593:Benzyl Compounds / Q000494:pharmacology; D001599:Berberine / Q000302:isolation & purification* / Q000378:metabolism*; D028142:Berberis / Q000254:growth & development* / Q000378:metabolism*; D018929:Cell Culture Techniques; D010671:Phenylurea Compounds / Q000494:pharmacology; D010846:Picloram / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development* / Q000378:metabolism*; D011687:Purines / Q000494:pharmacology; D013830:Thiadiazoles / Q000494:pharmacology
chemical_list: D001593:Benzyl Compounds; D010671:Phenylurea Compounds; D010937:Plant Growth Regulators; D011687:Purines; D013830:Thiadiazoles; C016785:thidiazuron; D001599:Berberine; C480551:benzylaminopurine; D010846:Picloram