Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing.
We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in approximately 20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from approximately 95% of multiexon genes undergo alternative splicing and that there are approximately 100,000 intermediate- to high-abundance alternative splicing events in major human tissues. From a comparison with quantitative alternative splicing microarray profiling data, we also show that mRNA-Seq data provide reliable measurements for exon inclusion levels.
Pan
Qun
Q
Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada.
Shai
Ofer
O
Lee
Leo J
LJ
Frey
Brendan J
BJ
Blencowe
Benjamin J
BJ
D017398:Alternative Splicing; D020869:Gene Expression Profiling; D006801:Humans; D022821:RNA Splice Sites; D012333:RNA, Messenger; D017423:Sequence Analysis, RNA / Q000379:methods*
D022821:RNA Splice Sites; D012333:RNA, Messenger