Temporal proteomic analysis of intestine developing necrotizing enterocolitis following enteral formula feeding to preterm pigs.
Necrotizing enterocolitis (NEC), a serious gastrointestinal inflammatory disease, frequently occurs in preterm neonates that fail to adapt to enteral nutrition. A temporal gel-based proteomics study was performed on porcine intestine with NEC lesions induced by enteral formula feeding. Functional assignment of the differentially expressed proteins revealed that important cellular functions, such as the heat shock response, protein processing; and purine, nitrogen, energy metabolism, were possible involved in the early progression of NEC.
Hang
Pingping
P
Division of Agricultural, Food and Nutritional Science, School of Biological Sciences, The University of Hong Kong, China.
Sangild
Per T
PT
Sit
Wai-Hung
WH
Ngai
Heidi Hoi-Yee
HH
Xu
Ruojun
R
Siggers
Jayda Lee Ann
JL
Wan
Jennifer Man-Fan
JM
D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D004750:Enteral Nutrition / Q000009:adverse effects*; D020345:Enterocolitis, Necrotizing / Q000175:diagnosis* / Q000378:metabolism* / Q000662:veterinary; D020869:Gene Expression Profiling / Q000379:methods; D007091:Image Processing, Computer-Assisted; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000473:pathology; D013058:Mass Spectrometry / Q000379:methods; D018529:Nutritional Support / Q000009:adverse effects*; D047928:Premature Birth; D040901:Proteomics / Q000379:methods*; D013552:Swine; D013997:Time Factors