Resolution of salbutamol enantiomers in human urine by reversed-phase high performance liquid chromatography after derivatization with (S)-(-)-alpha-methylbenzyl isocyanate.
A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with (S)-(-)-alpha-methylbenzyl isocyanate, the diastereomeric derivatives were resolved (R(s)=1.59) on 5 mum octadecylsilan column using 47% methanol as a mobile phase with fluorescence detection. The detection limit of each enantiomer was 10 ng/ml (S/N=3).
Kim
K H
KH
College of Pharmacy, Kangwon National University, 200-701, Chunchon, Korea.
Kim
T K
TK
Kwon
Y H
YH
Sohn
Y T
YT