A validated and fast procedure for FTIR determination of Cypermethrin and Chlorpyrifos.
A FTIR methodology has been developed for the simultaneous determination of Cypermethrin and Chlorpyrifos in pesticide commercially available formulations. The method involves the extraction of both active principles with CHCl(3) and direct measurement of the peak area values between 1747 and 1737cm(-1) corrected with a baseline defined at 2000cm(-1) for Cypermethrin and peak height values established at 1549cm(-1) corrected using a baseline situated at 1650cm(-1) for Chlorpyrifos. The limits of detection achieved were of the order of 0.7 and 0.4% (w/w), and the relative standard deviation 0.4 and 0.2% for Cypermethrin and Chlorpyrifos, respectively. The developed procedure provided statistically comparable results with those obtained by HPLC, for a series of commercial samples, which validated the FTIR method. The procedure developed reduces organic solvent consumption, per sample preparation, from 51ml CH(3)CN required for HPLC to 2.5ml CHCl(3), and reduces waste generation also increasing the sample measurement frequency, from 3 to 30 samples/h, as compared with the HPLC-UV reference method.
Armenta
Sergio
S
Department of Analytical Chemistry, University of Valencia, Edifici Jeroni Muñoz, 50th Dr. Moliner, 46100 BURJASSOT, Valencia, Spain.
Quintás
Guillermo
G
Garrigues
Salvador
S
de la Guardia
Miguel
M