Spectrofluorimetric determination of nitric oxide at trace levels with 5,6-diamino-1,3-naphthalene disulfonic acid.
Based on the selective reaction that 5,6-diamino-1,3-naphthalene disulfonic acid (DANDS) traps nitric oxide (NO) in the presence of dioxygen to yield the highly fluorescent form, 1-[H]-naphthotriazole-6,8-disulfonic acid in moderately alkaline medium, a new spectrofluorimetric method for the determination of NO has been reported. The method offered the advantage of specificity, sensitivity and a simple protocol for the direct detection of NO in aqueous solution. The linear calibration range for NO was 0.04-1.44 mumoll(-1) with a 3sigma detection limit of 0.6 nmoll(-1). The proposed method has been used to monitor the release of NO from S-nitrosocysteine, a NO-releasing agent.
Zhang
Xian
X
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Wang
Hong
H
Liang
Shu-Cai
SC
Zhang
Hua-Shan
HS