In vitro gentamicin sustained and controlled release from chitosan cross-linked films.
A novel wound dressing film was investigated for controlled and sustained delivery of gentamicin, while covering and protecting the wound. Chitosan was cross-linked with Hexamethylene 1,6-bis (aminocarboxysulfonate) to prepare the wound dressing films. Cross-linking reaction was required to control the water retention and release of encapsulated gentamicin. Cross-linked films swell less and became more hydrophilic than chitosan film itself. However, this behavior was inversely proportional to the degree of cross-linking. In vitro gentamicin release from the cross-linked films, at physiological conditions of pH and temperature, was studied for 2 weeks. The effects of gentamicin initial concentration and cross-linking ratio on the kinetics of gentamicin release were evaluated. Results showed that the diffusion rate was governed by initial concentration of gentamicin and degree of cross-linking, since higher gentamicin initial concentration and degree of cross-linking promoted the slower release, while lower gentamicin initial concentration and degree of cross-linking promoted the faster one.
Campos
Maria G N
MG
Department of Restorative Dentistry, Biomaterials Division, Dental School, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA. nogueiracamp@gmail.com
Rawls
Henry R
HR
Innocentini-Mei
Lucia H
LH
Satsangi
Neera
N
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004058:Diffusion; D005839:Gentamicins / Q000008:administration & dosage* / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial
D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D005839:Gentamicins; D008567:Membranes, Artificial