title: A new competitive enzyme-linked immunosorbent assay (ELISA) for determination of estrogenic bisphenols.
abstract: Bisphenol A and other hydroxylated diphenylalkanes (generally known as bisphenols) have been identified as potential estrogenic substances. In this paper, a specific polyclonal antibody was produced against these compounds by immunization of rabbits with a conjugate of 4,4-bis (4-hydroxyphenyl) valeric acid and bovine serum albumin (BHPVA-BSA). The polyclonal antibody showed specific recognition of the bisphenol structure, while the cross reactions of other common phenolic compounds such as phenol, hydroquinol and p-hydroxybenzoic acid were all lower than 1%. The linear range of bisphenol A calibration curve was 1-10 000 ng ml(-1). Real water samples and mouse serum samples were spiked with known amount of bisphenol A and measured by the competitive ELISA. Recoveries were between 92 and 105%. The detection limits were found to be 0.1 ng ml(-1) for real water samples and 2 ng ml(-1) for serum samples. The method is very useful for monitoring bisphenol compounds in environmental and biological samples.
authors.affiliation: The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Mei-Ping
authors.initials: MP
authors.lastname: Zhao
authors.forename: Yuan-Zong
authors.initials: YZ
authors.lastname: Li
authors.forename: Zhen-Quan
authors.initials: ZQ
authors.lastname: Guo
authors.forename: Xin-Xiang
authors.initials: XX
authors.lastname: Zhang
authors.forename: Wen-Bao
authors.initials: WB
authors.lastname: Chang