title: [Transfection with human adenovirus type 5 early-region 1A protects the primary human fibroblasts from ras-induced premature senescence in vitro].
abstract: OBJECTIVE
  To investigate the role of human adenovirus type 5 (Ad5) early-region 1A (E1A) in premature senescence of human fibroblasts induced by Ras activation.
  
  
  METHODS
  Human fibroblasts were cotransduced with E1A, E1A1-143, or their vector (BP) and Ha-RasV12 or its vector (WH). The growth curves and percentages of the apoptotic cells were determined.
  
  
  RESULTS
  Expression of E1A or Ha-RasV12 in human fibroblasts significantly inhibited the cell growth. Transduction of Ha-RasV12 along with E1A or E1A 1-143 into human fibroblast cells resulted in active and rapid cell proliferation.
  
  
  CONCLUSIONS
  E1A can rescue human fibroblasts from Ras-induced premature senescence, and the senescence bypassing activity of E1A resides in its NH2 terminus.
authors.affiliation: Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lei@fimmu.com
authors.forename: Yi-lei
authors.initials: YL
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.forename: Chong-yuan
authors.initials: CY
authors.lastname: Xu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yan
mesh_terms: D017500:Adenovirus E1A Proteins / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D011905:Genes, ras / Q000502:physiology*; D006801:Humans; D012867:Skin / Q000166:cytology; D014162:Transfection
chemical_list: D017500:Adenovirus E1A Proteins