Fluorimetric determination of indole derivatives by fluorophore generation in copper(II)-sulphuric acid solution.
A spectrofluorimetric procedure for the determination of indole-3-acetic acid, indole-3-propanoic acid and indole-3-butyric acid by derivatization with copper sulphate-sulphuric acid solution has been developed. The optimum reaction conditions, the effect of interferents and the advantages associated with the use of first- and second-derivative synchronous spectrofluorimetry have been studied. The detection limits are 3, 12 and 6 ng/ml for indole-3-acetic, indole-3-propanoic and indole-3-butyric acid, respectively.
Sanchez
F G
FG
Department of Analytical Chemistry, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain.
Ruiz
C C
CC
Bayona
A H
AH