title: Preparation and anti-tumor efficiency evaluation of doxorubicin-loaded bacterial magnetosomes: magnetic nanoparticles as drug carriers isolated from Magnetospirillum gryphiswaldense.
abstract: Bacterial magnetosomes (BMs) are commonly used as vehicles for certain enzymes, nucleic acids and antibodies, although they have never been considered drug carriers. To evaluate the clinical potential of BMs extracted from Magnetospirillum gryphiswaldense in cancer therapy, doxorubicin (DOX) was loaded onto the purified BMs at a ratio of 0.87 +/- 0.08 mg/mg using glutaraldehyde. The DOX-coupled BMs (DBMs) and BMs exhibited uniform sizes and morphology evaluated by TEM. The diameters of DBMs and BMs obtained by AFM were 71.02 +/- 6.73 and 34.93 +/- 8.24 nm, respectively. The DBMs released DOX slowly into serum and maintained at least 80% stability following 48 h of incubation. In vitro cytotoxic tests showed that the DBMs were cytotoxic to HL60 and EMT-6 cells, manifested as inhibition of cell proliferation and suppression in c-myc expression, consistent with DOX. These observations depicted in vitro antitumor property of DBMs similar to DOX. The approach of coupling DOX to magnetosomes may have clinical potential in anti-tumor drug delivery.
authors.affiliation: State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing, PR China.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Sun
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Duan
authors.forename: Shun-Ling
authors.initials: SL
authors.lastname: Dai
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004317:Doxorubicin / Q000378:metabolism* / Q000494:pharmacology*; D004337:Drug Carriers / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008280:Magnetics; D042201:Magnetospirillum / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D004317:Doxorubicin