title: Association between variants in the 5'-untranslated region of the bovine MC4R gene and two growth traits in Nanyang cattle.
abstract: Melanocortin-4 receptor (MC4R) is one of five G-protein-coupled receptors binding melanocortins that is implicated in the control of feeding behavior and energy homeostasis. Six cattle populations (n = 594), including four Chinese indigenous breeds, Chinese Holstein, and a meat type breed (Angus), were used to detect single nucleotide polymorphisms in 5'-untranslated region of MC4R gene by means of PCR-SSCP and DNA sequencing. Four linked SNPs (g.[-293C>G; -193A>T; -192T>G; -129A>G]) were identified. The g.-293C>G and g.-129A>G could be genotyped with a PCR-RFLP using TaiI in three combined genotypes (AA, AB and BB). The two linked SNPs were associated with body weight and daily gain in Nanyang aged 6 months (P < 0.05), but they had no significant effect on body weight and daily gain in Nanyang aged 24 months (P > 0.05).
authors.affiliation: College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi, People's Republic of China. clzhang_xz@126.com
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Zhang
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Xian-Yong
authors.initials: XY
authors.lastname: Lan
authors.forename: Chu-Zhao
authors.initials: CZ
authors.lastname: Lei
authors.forename: Xing-Tang
authors.initials: XT
authors.lastname: Fang
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D002681:China; D004587:Electrophoresis, Agar Gel; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006579:Heterozygote; D016018:Least-Squares Analysis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D019655:Quantitative Trait, Heritable; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D020121:5' Untranslated Regions; D044105:Receptor, Melanocortin, Type 4