[DNA polymorphism detection of Cannabis using amplified fragment length polymorphism].
OBJECTIVE
To screen the AFLP primers with good diversity to distinguish various species of Cannabis.


METHODS
The AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations.


RESULTS
A total of 285 AFLP bands were obtained using five primer combinations with better diversity, among which 99 bands were polymorphic and 10 bands were special, with 47-76 bands amplified in each pair of primers.


CONCLUSIONS
AFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.
Guo
Jia
J
Chinese People's Public Security University, Beijing 100038, China. guojia99@tom.com
Pei
Li
L
Peng
Jian-Xiong
JX
Zhai
Hong
H
Zhang
Gui-Qin
GQ
Jie
Qin
Q
Tu
Zheng
Z
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D002188:Cannabis / Q000145:classification / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D053803:Forensic Genetics; D014644:Genetic Variation; D011110:Polymorphism, Genetic
D018744:DNA, Plant