title: Leucobacter tardus sp. nov., isolated from the phyllosphere of Solanum tuberosum L.
abstract: In the course of studying the composition of the microbial community in the phyllosphere of potato plants, a Gram-positive bacterium, strain K 70/01(T), was isolated. On the basis of 16S rRNA gene sequence analysis, it was shown that the investigated isolate represents a novel species in the genus Leucobacter; the closest phylogenetic neighbour was Leucobacter chromiireducens subsp. chromiireducens, with a sequence similarity of 96.8 % to the type strain. Chemotaxonomic characteristics supported affiliation to the genus Leucobacter. Strain K 70/01(T) displayed the diagnostic diamino acid 2,4-diaminobutyric acid in the cell-wall peptidoglycan. The major fatty acids were anteiso-C(15 : 0,) anteiso-C(17 : 0) and iso-C(16 : 0), which are typical of the genus. The quinone system consisted of the menaquinones MK-10, MK-11 and MK-9. Differences in several physiological features and the absence of the cell-wall amino acids gamma-aminobutyric acid and threonine enabled the isolate to be differentiated from all recognized Leucobacter species. Based on these phylogenetic and phenotypic results, it can be concluded that the isolate from the phyllosphere of potato plants represents a novel species, for which the name Leucobacter tardus sp. nov. is proposed. The type strain is K 70/01(T) (=DSM 19811(T) =LMG 24388(T)).
authors.affiliation: Leibniz-Centre for Agricultural Landscape Research (ZALF), Institute of Landscape Matter Dynamics, Eberswalder Str. 84, D-15374 Müncheberg, Germany. ubehrendt@zalf.de
authors.forename: Undine
authors.initials: U
authors.lastname: Behrendt
authors.forename: Andreas
authors.initials: A
authors.lastname: Ulrich
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D000613:Aminobutyrates / Q000032:analysis; D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010641:Phenotype; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D000613:Aminobutyrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; C005959:2,4-diaminobutyric acid