title: Immobilized hyaluronic acid containing model silicone hydrogels reduce protein adsorption.
abstract: The physical immobilization of hyaluronic acid (HA) as a wetting agent in contact lens applications was investigated using model silicone hydrogels prepared by co-polymerizing methacryloxy propyl tris (trimethylsiloxy) silane (Tris) and hydroxyethylmethacrylate (HEMA). Two different molecular weights of HA were investigated, as well as various Tris/HEMA ratios. Cross-linked HA, while only present in small amounts, increased water uptake and hydrophilicity in the materials containing 10% Tris; however, no changes were observed in the 4% Tris materials. The presence of the HA was further confirmed by increases in glass transition temperature as measured by differential scanning calorimetry. Lysozyme adsorption was dramatically decreased for materials containing cross-linked HA. The incorporation of HA, previously demonstrated to decrease protein adsorption to model conventional hydrogel materials, may have significant potential for improving the wetting and other properties of silicone hydrogel contact lens materials.
authors.affiliation: Department of Chemical Engineering, McMaster University, 1280 Main St. West, Hamilton, Ontario, Canada.
authors.forename: Mark
authors.initials: M
authors.lastname: van Beek
authors.forename: Andrea
authors.initials: A
authors.lastname: Weeks
authors.forename: Lyndon
authors.initials: L
authors.lastname: Jones
authors.forename: Heather
authors.initials: H
authors.lastname: Sheardown
mesh_terms: D000327:Adsorption / Q000187:drug effects; D002152:Calorimetry, Differential Scanning; D003261:Contact Lenses; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology*; D020100:Hydrogels / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry*; D020034:Silicone Gels / Q000737:chemistry*; D013057:Spectrum Analysis; D014867:Water / Q000737:chemistry; D014906:Wetting Agents / Q000737:chemistry* / Q000494:pharmacology*; D014965:X-Rays
chemical_list: D020100:Hydrogels; D011506:Proteins; D020034:Silicone Gels; D014906:Wetting Agents; D014867:Water; D006820:Hyaluronic Acid; D009113:Muramidase