title: Fluorescence energy transfer between Acridine Orange and Safranine T and its application in the determination of DNA.
abstract: The fluorescence energy transfer (FET) between Acridine Orange and Safranine T, two intercalators of DNA, was studied in this paper. The FET efficiency between Acridine Orange and Safranine T is higher and the critical distance, R(0), is longer in the intercalated state than in the free one. A new method for the determination of calf thymus DNA (ctDNA) was presented. The linear range of the calibration curve is (0 approximately 1.1)x10(-5) mol l(-1) in bases for ctDNA, and the limit of detection is 2.6x10(-7) mol l(-1).
authors.affiliation: Department of Chemistry, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.forename: X
authors.initials: X
authors.lastname: He
authors.forename: Z
authors.initials: Z
authors.lastname: Gao
authors.forename: L
authors.initials: L
authors.lastname: Peng