Inhibition of the epstein-barr virus lytic cycle by andrographolide.
Andrographis paniculata NEES is a medicinal plant that is commonly used in Asia. This work demonstrates that 25 microg/ml of ethanolic extract from A. paniculata (EEAP) and 5 microg/ml of andrographolide, a bioactive compound in EEAP, effectively inhibit the expression of Epstein-Barr virus (EBV) lytic proteins, Rta, Zta and EA-D, during the viral lytic cycle in P3HR1 cells. Transient transfection analysis revealed that the lack of expression of Rta, Zta and EA-D is caused by the inhibition of the transcription of BRLF1 and BZLF1, two EBV immediate-early genes that encode Rta and Zta, respectively. This study finds that the inhibition prevents the virus from producing mature viral particles. Meanwhile, andrographolide is not toxic to P3HR1 cells when the concentration is below 5 microg/ml, indicating that the compound is potentially useful as an anti-EBV drug.
Lin
Tsuey-Pin
TP
Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, Taiwan. tplin007@mail.chna.edu.tw
Chen
Shih-Ying
SY
Duh
Pin-Der
PD
Chang
Li-Kwan
LK
Liu
Yen-Ni
YN
D035685:Andrographis / Q000737:chemistry; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D005434:Flow Cytometry; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D017781:Genes, Immediate-Early / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006801:Humans; D035261:Plant Components, Aerial / Q000737:chemistry; D014158:Transcription, Genetic / Q000187:drug effects; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014771:Virion / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D017735:Virus Latency / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004224:Diterpenes; D014764:Viral Proteins; C030419:andrographolide