Proteomic analysis of secreted membrane vesicles of archaeal Sulfolobus species reveals the presence of endosome sorting complex components.
The crenarchaea Sulfolobus acidocaldarius, S. solfataricus and S. tokodaii, release membrane vesicles into the medium. These membrane vesicles consist of tetraether lipids and are coated with an S-layer. A proteomic analysis reveals the presence of proteins homologous to subunits of the eukaryotic endosomal sorting complex required for transport (ESCRT). Immunodetection of one of these homologs suggest a cell surface localization in intact cells. These data suggest that the membrane vesicles in Sulfolobus sp. emerge from a specific budding process with similarity to the endosomal sorting pathway.
Ellen
Albert F
AF
Department of Molecular Microbiology Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
Albers
Sonja-Verena
SV
Huibers
Wim
W
Pitcher
Angela
A
Hobel
Cedric F V
CF
Schwarz
Heinz
H
Folea
Mihaela
M
Schouten
Stefan
S
Boekema
Egbert J
EJ
Poolman
Bert
B
Driessen
Arnold J M
AJ
D019843:Archaeal Proteins / Q000378:metabolism*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D011992:Endosomes / Q000378:metabolism*; D008854:Microscopy, Electron; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016944:Sulfolobus / Q000378:metabolism*
D019843:Archaeal Proteins