title: Measurement of serum carboxyterminal cross-linked telopeptide of type I collagen concentration in dogs with osteosarcoma.
abstract: OBJECTIVE
  To evaluate the usefulness of carboxyterminal cross-linked telopeptide of type I collagen (ICTP) concentrations for screening dogs for the presence of osteosarcoma.
  
  
  METHODS
  32 client-owned dogs with osteosarcoma (27 dogs with osteosarcoma of the appendicular skeleton and 5 dogs with osteosarcoma of the axial skeleton) and 44 non-tumor-bearing control dogs.
  
  
  METHODS
  Serum was obtained from blood samples collected from dogs with osteosarcoma and from clinically normal dogs. The serum ICTP concentration was determined by use of a commercially available radioimmunoassay for ICTP.
  
  
  RESULTS
  Mean +/- SD serum ICTP concentration in the tumor-bearing dogs was 7.32 +/- 2.88 ng/mL, and in clinically normal dogs, it was 6.77 +/- 2.31 ng/mL; values did not differ significantly. Mean serum ICTP concentration in dogs with appendicular osteosarcoma, compared with that of clinically normal dogs, was not significantly different. Mean serum ICTP concentration in dogs with axial skeletal tumor location was 10.82 +/- 2.31 ng/mL, compared with a value of 6.73 +/- 2.28 ng/mL in dogs with appendicular osteosarcoma.
  
  
  CONCLUSIONS
  On the basis of the results of this study, serum ICTP concentrations are not a clinically useful screening tool for the detection of appendicular osteosarcoma in dogs. Despite the observation that serum ICTP concentration was higher in dogs with axial osteosarcoma than in clinically normal dogs, serum ICTP concentration determination is not a suitable screening test for osteosarcoma.
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.
authors.forename: John G
authors.initials: JG
authors.lastname: Hintermeister
authors.forename: Pamela D
authors.initials: PD
authors.lastname: Jones
authors.forename: Walter E
authors.initials: WE
authors.lastname: Hoffmann
authors.forename: Arthur M
authors.initials: AM
authors.lastname: Siegel
authors.forename: Nikolaos G
authors.initials: NG
authors.lastname: Dervisis
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Kitchell
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D024042:Collagen Type I; D004283:Dog Diseases / Q000097:blood* / Q000175:diagnosis*; D004285:Dogs; D016015:Logistic Models; D012516:Osteosarcoma / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D010446:Peptide Fragments / Q000097:blood*; D010455:Peptides; D011347:Procollagen / Q000097:blood*; D011863:Radioimmunoassay / Q000662:veterinary
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D010446:Peptide Fragments; D010455:Peptides; D011347:Procollagen; C052929:collagen type I trimeric cross-linked peptide