Synthesis of DNA strands site-specifically damaged by c8-arylamine purine adducts and effects on various DNA polymerases.
C8-Arylamine-dG and C8-arylamine-dA adducts have been prepared using palladium cross-coupling chemistry. These adducts were subsequently converted into the corresponding 5'-O-DMTr-C8-arylamine-3'-O-phosphoramidites and then used for the automated synthesis of different site-specifically modified oligonucleotides. These "damaged" oligonucleotides have been characterized by ESI-MS, UV thermal stability assays, and circular dichroism, and they have been used in EcoRI assays as well as in primer extension studies using various DNA polymerases.
Böge
Nicolas
N
Organic Chemistry, Department of Chemistry, Faculty of Science, University of Hamburg, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany.
Jacobsen
Maike I
MI
Szombati
Zita
Z
Baerns
Sabrina
S
Di Pasquale
Francesca
F
Marx
Andreas
A
Meier
Chris
C
D018736:DNA Adducts / Q000138:chemical synthesis*; D004249:DNA Damage; D004259:DNA-Directed DNA Polymerase / Q000187:drug effects*; D015246:Deoxyribonuclease EcoRI; D021141:Nucleic Acid Amplification Techniques; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds; D010165:Palladium; D011687:Purines / Q000737:chemistry*; D013057:Spectrum Analysis
D018736:DNA Adducts; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; D011687:Purines; C434331:phosphoramidite; D010165:Palladium; D004259:DNA-Directed DNA Polymerase; D015246:Deoxyribonuclease EcoRI; C030985:purine