[A study on differentially expressed genes in hepatic stellate cells treated with transforming growth factor beta 1 using cDNA microarray technique].
OBJECTIVE
To screen the differentially expressed genes in hepatic stellate cells (HSC) treated with transforming growth factor beta 1 (TGFbeta1) by cDNA microarray technique, and to elucidate the molecular pathogenesis of liver fibrosis involving TGFb1.


METHODS
Total RNA was extracted from HSC treated with TGFbeta1 and PBS by trizol and reverse-transcribed to double strand cDNA templates. Transcription of cDNA probe with biotin-labeling was performed, and then the obtained cDNA was hybridized with human cDNA microarray. The results were imaged by an Agilent scanner, and the differentially expressed genes were analyzed with bioinformatics software.


RESULTS
One hundred seventy-seven differentially expressed genes were screened from 13824 targeting genes; 123 genes were up-regulated, including connective tissue growth factor, tubulin epsilon 1, collagen, type V, alpha2, catenin delta 2, cadherin 6, type 2, Smad3, mitogen-activated protein kinase 4, growth factor receptor-bound protein 7 and MAP kinase-interacting serine/threonine kinase 1; 54 genes were down-regulated, including TNF receptor-associated factor 4, interferon regulatory factor 7, interferon inducible protein p78, bone morphogenetic protein 7, matrix gla protein, serine proteinase inhibitor, interferon stimulated gene 2.0 x 10(4), death-associated protein 6, metallothionein 1H and superoxide dismutase 2; in addition, 8 genes with unknown functions were also found.


CONCLUSIONS
The differentially expressed genes in HSC treated with TGFbeta1 were successfully screened by cDNA microarray technique. It revealed that the molecular pathogenesis of liver fibrosis involving TGFbeta1 was the result of co-regulation by multiple factors. This information might be of help in searching for new targets in gene therapy.
Xiao
Lin
L
Department of Infectious Diseases, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China.
Cheng
Jun
J
Guo
Hong
H
Hong
Yuan
Y
Zhang
Li-ying
LY
Zhang
Yue-xin
YX
Zhang
Jian-long
JL
Li
Yan
Y
D000818:Animals; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D055166:Hepatic Stellate Cells; D008103:Liver Cirrhosis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
D053773:Transforming Growth Factor beta1