title: Pluronic F-127 as a cell carrier for bone tissue engineering.
abstract: The objective of this study is to report the effect of Pluronic F-127 on osteoblast viability and phenotype maintenance in vitro. MG-63 cells are suspended in Pluronic F-127, and MTT assay, alkaline phosphatase activity, prostaglandin E(2) production, collagen-I, and cyclo-oxygenase-2 expression are assessed up to 6 days. Pluronic F-127 leads to a significant decrease in osteoblast viability throughout the 6-day experiment, without altering osteoblast phenotype. The addition of platelet-rich plasma to the polymer/cell construct leads to increased cell survival. When supplemented with bioactive factors, Pluronic F-127 could potentially be used as a cell carrier in bone tissue engineering.
authors.affiliation: Orthopaedic Research Laboratory, Department of Orthopaedics, Centre hospitalier Sacré-Coeur, Montréal, Québec, Canada.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Brunet-Maheu
authors.forename: Julio C
authors.initials: JC
authors.lastname: Fernandes
authors.forename: Clemente A V
authors.initials: CA
authors.lastname: de Lacerda
authors.forename: Qin
authors.initials: Q
authors.lastname: Shi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Benderdour
authors.forename: Patrick
authors.initials: P
authors.lastname: Lavigne
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D020442:Poloxamer / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D018786:Bone Substitutes; D020442:Poloxamer