Quantification of sex pheromone from Anocentor nitens by gas chromatography-mass spectrometry-selected ion monitoring.
Two highly sensitive and selective methods based on gas chromatography coupled to mass spectrometry (GC-MS) in the selected ion monitoring (SIM) mode have been developed for the quantification of 2,6-dichlorophenol (2,6-DCP), a sex pheromone of the tick females of Anocentor nitens. Standard addition method and calibration curve techniques using 5-bromine-4-hydroxy-3-methoxybenzaldehyde (5-BrV) as internal standard (IS) afforded detection limit of 0.1ngml(-1). The calibration curve was linear over the concentration range from 0.5 to 500ngml(-1) for 2,6-DCP. Results show that the concentration range of sex pheromone in the extracts samples was 1.08-10.35ngml(-1). The methods developed provided reliable procedures to determine amounts of 2,6-DCP present in ticks.
Rodrigues
Clenilson M
CM
Instituto de Quimica, Universidade Federal de Goiás, C.P. 131, 74001-970 Goiânia, GO, Brazil.
Borges
Lígia M F
LM
Ferri
Pedro H
PH
Faria
Lourival C
LC