Inorganic salts as hold-up time markers in C(18) columns.
A systematic study of the retention behaviour in a C(18) reversed phase liquid chromatography (RPLC) system of several inorganic salts, namely NaNO(2), NaNO(3), KBr, Bu(4)NBr and K(2)Cr(2)O(7), is presented. The behaviour of the markers in unbuffered mobile phases and the marker retention dependence on the pH and ionic strength of the buffered mobile phases have been analyzed. In addition, a comparison between the retention behaviour of the markers and several ionizable analytes in buffered eluents is presented.
Oumada
F Z
FZ
Departament de Quimica Analitica, Universitat de Barcelona, Avda Diagonal 647, 08028 Barcelona, Spain.
Rosés
M
M
Bosch
E
E