title: Pax9 mediated cell survival in oral squamous carcinoma cell enhanced by c-myb.
abstract: Paired box gene 9 (Pax9) and c-myb are transcription factors that regulate the expression of the genes involved in mediating cell proliferation, resistance to apoptosis, and migration. However, the function of Pax9 in oral squamous cell carcinoma (OSCC) is virtually unknown. This study examined the anti-apoptotic roles of Pax9 and c-myb, and clarified interaction between the two genes in KB cells. Inhibition of Pax9 caused the induction of apoptosis with enhanced cleavage of caspase-3 and PARP, accelerated Bax, and reduced Bcl-2 expression. Transducing c-myb cells with adenovirus c-myb (Ad/c-myb) were induced cell growth and inhibited apoptosis, but dominant-negative myb cells (Ad/DN-myb) were not affected. Pax9 was upregulated in the Ad/c-myb cells with simultaneous decrease in the Ad/DN-myb infection. However, c-myb remained unaffected in the Pax9 small interfering RNA (siRNA) transfected cells. Moreover, the Pax9 siRNA transfected cells and Ad/DN-myb infected cells were able to arrest the cell cycle at the G(0) phase. This suggests that Pax9 and c-myb expression in KB cells is essential for cell growth, and survival is enhanced by c-myb. Disrupting the function of c-myb and Pax9 could be a potential target for cancer treatment.
authors.affiliation: Department of Oral Biochemistry, Institute of Oral-Bio Science, School of Dentistry, Chonbuk National University, Joenju, Korea.
authors.forename: Jung-Chang
authors.initials: JC
authors.lastname: Lee
authors.forename: Manju
authors.initials: M
authors.lastname: Sharma
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lee
authors.forename: Nan-Hee
authors.initials: NH
authors.lastname: Lee
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Ji-Soo
authors.initials: JS
authors.lastname: Yun
authors.forename: Sang-Yun
authors.initials: SY
authors.lastname: Nam
authors.forename: Pyoung-Han
authors.initials: PH
authors.lastname: Hwang
authors.forename: Eun-Chung
authors.initials: EC
authors.lastname: Jhee
authors.forename: Ho-Keun
authors.initials: HK
authors.lastname: Yi
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D007624:KB Cells; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism; D051764:PAX9 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D016192:Resting Phase, Cell Cycle; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051764:PAX9 Transcription Factor; C497323:PAX9 protein, human; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger; D034741:RNA, Small Interfering