Assessment in vitro of the active hemostatic properties of wound dressings.
The development of actively hemostatic wound dressings for use in severe trauma remains a major public-health and military goal. But, although some manufacturers claim that existing dressings activate platelets and/or blood coagulation, mechanistic evidence is often lacking. We describe a method for assessing the active hemostatic properties of dressings in vitro, entailing measurement of the flow of recalcified platelet-rich plasma (PRP) through a dressing sample. If the dressing is hemostatically active, flow is reduced. This flow is then compared with the flow-through of PRP in which both platelet and coagulation function are blocked with EDTA. The ratio of the two generates a hemostatic index that ranges from 1.0 (no active hemostasis) to 0 (highly potent). The method is applicable to porous or semiporous dressings, whether fabric, sponge, fleece, or granules. For an active dressing, the test is easily modified to differentiate between the contributions of platelet and coagulation to overall hemostasis. The method is illustrated for fabrics, over-the-counter gauze and sponge dressings, collagen-based sheets, and an absorbent granule dressing. One active collagen dressing is used to illustrate discrimination between platelet and coagulation function. The ability to assess hemostatic properties may significantly enhance the development of advanced active dressings.
Jesty
Jolyon
J
Division of Hematology and Oncology, Health Sciences Center, Stony Brook University, Stony Brook, New York 11794-8151.
Wieland
Martin
M
Dr. Suwelack AG, Billerbeck, Germany.
Niemiec
Jack
J
Suwelack Matrix Systems Inc., Long Island High Technology Incubator, Stony Brook, New York 11790.
D000925:Anticoagulants / Q000494:pharmacology; D001458:Bandages; D001681:Biological Assay / Q000379:methods; D001777:Blood Coagulation / Q000187:drug effects*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D006487:Hemostasis / Q000187:drug effects / Q000502:physiology; D006489:Hemostatic Techniques; D006490:Hemostatics / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D053657:Platelet-Rich Plasma / Q000378:metabolism
D000925:Anticoagulants; D006490:Hemostatics; D004492:Edetic Acid