title: Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material.
abstract: A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon-10(-9) mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.
authors.affiliation: Department of Pure and Applied Biochemistry, Centre for Chemistry and Chemical Engineering, Lund University, S-221 00 Lund, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Khayyami
authors.forename: M T
authors.initials: MT
authors.lastname: Pérez Pita
authors.forename: N
authors.initials: N
authors.lastname: Peña Garcia
authors.forename: G
authors.initials: G
authors.lastname: Johansson
authors.forename: B
authors.initials: B
authors.lastname: Danielsson
authors.forename: P O
authors.initials: PO
authors.lastname: Larsson