title: Chromosomal imbalances are associated with increased proliferation and might contribute to bone destruction in cholesteatoma.
abstract: OBJECTIVE
  Our aim was to evaluate the copy number alterations of chromosomes 3, 7, 8, and 17 in middle ear cholesteatomas and define the association between the rate of cell proliferation and chromosome number changes.
  
  
  METHODS
  Tissues were obtained from 16 patients. Fluorescence in situ hybridization was performed on tumor imprint preparations. Cell proliferation was characterized with Ki-67 monoclonal antibody on cholesteatoma samples and on postauricular skins as control.
  
  
  RESULTS
  Different degrees of aneusomy were found for all chromosomes except for chromosome 3. Chromosome copy number alterations were associated with elevated proliferative rate and related also with the aggressiveness of the lesions.
  
  
  CONCLUSIONS
  Based on our results, we assume that aneusomy of chromosomes 7, 8, and 17 might play an important role during invasion of the adjacent bony structures of cholesteatoma, as well as associate with increased cell proliferation activity, which might lead to the aggressive behavior of the tissue.
authors.affiliation: Department of Preventive Medicine, Faculty of Public Health, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Ecsedi
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Rákosy
authors.forename: Laura
authors.initials: L
authors.lastname: Vízkeleti
authors.forename: Attila
authors.initials: A
authors.lastname: Juhász
authors.forename: István
authors.initials: I
authors.lastname: Sziklai
authors.forename: Róza
authors.initials: R
authors.lastname: Adány
authors.forename: Margit
authors.initials: M
authors.lastname: Balázs
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D016022:Case-Control Studies; D049109:Cell Proliferation; D002648:Child; D018424:Cholesteatoma, Middle Ear / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008416:Mastoid / Q000473:pathology; D008875:Middle Aged; D009361:Neoplasm Invasiveness
chemical_list: D019394:Ki-67 Antigen