Isolation and characterisation of a delta5-fatty acid elongase from the marine microalga Pavlova salina.
The marine microalga Pavlova salina (Haptophyta, Pavlovophyceae) produces lipids containing approximately 50% n-3 long-chain polyunsaturated fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). A full-length cDNA sequence, designated PsElo5, was isolated from P. salina. Sequence alignment showed that the gene was homologous to corresponding ELO-type elongases from other microalgae. Heterologous expression of PsElo5 in yeast and in higher plants confirmed that it encodes a specific Delta5-elongase activity as predicted and, furthermore, within the n-3 pathway, the elongation activity was confined exclusively to EPA.
Robert
Stanley S
SS
Food Futures National Research Flagship, CSIRO Marine and Atmospheric Research, Hobart, Tasmania, Australia.
Petrie
James R
JR
Zhou
Xue-Rong
XR
Mansour
Maged P
MP
Blackburn
Susan I
SI
Green
Allan G
AG
Singh
Surinder P
SP
Nichols
Peter D
PD
D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D056890:Eukaryota / Q000201:enzymology*; D000081031:Fatty Acid Elongases; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis / Q000378:metabolism*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015003:Yeasts
D017931:DNA Primers; D018076:DNA, Complementary; D005231:Fatty Acids, Unsaturated; C445137:delta5 polyunsaturated fatty acid; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases