[Expression of FMNL2 and its relation to the metastatic potential of human colorectal cancer cells].
OBJECTIVE
To explore the association of FMNL2 expression with the metastatic potential of colorectal cancer cells.


METHODS
FMNL2 mRNA and protein expressions in 6 human colorectal cancer cell lines were detected by real-time RT-PCR and immunohistochemical method, respectively, and analyzed for their correlations to the in vitro invasiveness of the cell lines evaluated by Boyden assay. In SW620 and SW480/M5 cell lines, the expression of FMNL2 was repressed by FMNL2 short hairpin RNA (shRNA), and the changes in the invasiveness of the cells were observed.


RESULTS
FMNL2 was highly expressed in SW480/M5, LoVo and SW620 cells derived from metastatic colorectal cancers in comparison with that in LS174T, SW480 and HT29 cells, which were derived from primary colorectal cancers. In vitro analysis of the cell invasiveness demonstrated that SW480/M5, LoVo and SW620 cells had higher invasiveness than LS174T, SW480 and HT29 in vitro. In SW480/M5 and SW620 cells, transfection with FMNL2 shRNA resulted in significantly lowered cell invasiveness.


CONCLUSIONS
FMNL2 may play an important role in the invasion and metastasis of colorectal cancer.
Zhu
Xi-ling
XL
Department of Pathology, Southern Medical University, Guangzhou 510515, China. zmgw @fimmu.com
Liang
Li
L
Ding
Yan-qing
YQ
D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D000080865:Formins; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014407:Tumor Cells, Cultured
C476208:FMNL2 protein, human; D000080865:Formins; D011506:Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering