Specific rotation measurements from peak height data, with a Gaussian peak model.
A method is described whereby a sensitive laser-based polarimeter can be used to make very accurate and precise specific rotation measurements on microgram quantities of optically active materials. Flow-injection or liquid chromatography systems provide reproducible introduction of the sample into the polarimetric system. If a Gaussian distribution of the analyte concentration is assumed, the peak height can be used in the determination of specific rotation. This method provides a direct calibration with an absolute standard which yields more accurate and precise results than those obtainable by using peak area.
Rice
P D
PD
Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, U.S.A.
Shao
Y Y
YY
Erskine
S R
SR
Teague
T G
TG
Bobbitt
D R
DR