title: Duddingtonia flagrans: centrifugal flotation technique with magnesium sulphate for the quantification and qualification of chlamydospores in sheep faeces.
abstract: Duddingtonia flagrans is a nematode-trapping fungus responsible for attacking larval stages of helminths in pasture, which has potential as a biological control method. The aim of this study was to test the magnesium sulphate centrifugal flotation technique for the quantification of D. flagrans chlamydospores in sheep faeces and to verify their morphological viability. In this experiment one sheep received an oral dose of 4.5 x 10(6) chlamydospores/day during 20 days. Fecal samples were collected between days 15 and 20 and analyzed by the centrifugal flotation technique with magnesium sulphate. Densities of 1.23, 1.27 and 1.31gmL(-1) recovered 1.45 x 10(5), 3.87 x 10(5) and 1.65 x 10(5) chlamydospores from the faeces, respectively. Based upon the results it was concluded that this is an efficient technique for the chlamydospores quantification in ovine faeces. Moreover, it allowed more accurate visualization of chlamydospore morphology.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil.
authors.forename: Aleksandro Schafer da
authors.initials: AS
authors.lastname: Silva
authors.forename: Régis Adriel
authors.initials: RA
authors.lastname: Zanette
authors.forename: Mateus Anderson
authors.initials: MA
authors.lastname: Otto
authors.forename: Cláudia Dal Molin
authors.initials: CD
authors.lastname: Soares
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
authors.forename: Silvia Gonzalez
authors.initials: SG
authors.lastname: Monteiro
authors.forename: Janio Morais
authors.initials: JM
authors.lastname: Santurio
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000254:growth & development* / Q000302:isolation & purification; D002498:Centrifugation; D005243:Feces / Q000469:parasitology*; D005374:Filtration; D008278:Magnesium Sulfate; D008297:Male; D012756:Sheep; D013172:Spores, Fungal / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D008278:Magnesium Sulfate