Chemical engineering of mesenchymal stem cells to induce a cell rolling response.
Covalently conjugated sialyl Lewis X (SLeX) on the mesenchymal stem cell (MSC) surface through a biotin-streptavidin bridge imparts leukocyte-like rolling characteristics without altering the cell phenotype and the multilineage differentiation potential. We demonstrate that the conjugation of SLeX on the MSC surface is stable, versatile, and induces a robust rolling response on P-selectin coated substrates. These results indicate the potential to increase the targeting efficiency of any cell type to specific tissue.
Sarkar
Debanjan
D
Department of Medicine, Harvard-MIT Division of Heath Sciences and Technology, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA.
Vemula
Praveen Kumar
PK
Teo
Grace S L
GS
Spelke
Dawn
D
Karnik
Rohit
R
Wee
Le Y
le Y
Karp
Jeffrey M
JM
D001710:Biotin / Q000378:metabolism; D002454:Cell Differentiation; D002470:Cell Survival; D005453:Fluorescence; D006801:Humans; D036904:Leukocyte Rolling / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry; D019007:P-Selectin / Q000378:metabolism; D012680:Sensitivity and Specificity; D000080506:Sialyl Lewis X Antigen; D013194:Staining and Labeling; D019809:Streptavidin / Q000378:metabolism
D009844:Oligosaccharides; D019007:P-Selectin; D000080506:Sialyl Lewis X Antigen; D001710:Biotin; D019809:Streptavidin