title: CD133 is a marker of bioenergetic stress in human glioma.
abstract: Mitochondria dysfunction and hypoxic microenvironment are hallmarks of cancer cell biology. Recently, many studies have focused on isolation of brain cancer stem cells using CD133 expression. In this study, we investigated whether CD133 expression is regulated by bioenergetic stresses affecting mitochondrial functions in human glioma cells. First, we determined that hypoxia induced a reversible up-regulation of CD133 expression. Second, mitochondrial dysfunction through pharmacological inhibition of the Electron Transport Chain (ETC) produced an up-regulation of CD133 expression that was inversely correlated with changes in mitochondrial membrane potential. Third, generation of stable glioma cells depleted of mitochondrial DNA showed significant and stable increases in CD133 expression. These glioma cells, termed rho(0) or rho(0), are characterized by an exaggerated, uncoupled glycolytic phenotype and by constitutive and stable up-regulation of CD133 through many cell passages. Moreover, these rho(0) cells display the ability to form "tumor spheroids" in serumless medium and are positive for CD133 and the neural progenitor cell marker, nestin. Under differentiating conditions, rho(0) cells expressed multi-lineage properties. Reversibility of CD133 expression was demonstrated by transfering parental mitochondria to rho(0) cells resulting in stable trans-mitochondrial "cybrid" clones. This study provides a novel mechanistic insight about the regulation of CD133 by environmental conditions (hypoxia) and mitochondrial dysfunction (genetic and chemical). Considering these new findings, the concept that CD133 is a marker of brain tumor stem cells may need to be revised.
authors.affiliation: Department of Surgery, Division of Neurosurgery, University of Alabama at Birmingham, Birmingham, AL, USA. cgriguer@uab.edu
authors.forename: Corinne E
authors.initials: CE
authors.lastname: Griguer
authors.forename: Claudia R
authors.initials: CR
authors.lastname: Oliva
authors.forename: Eric
authors.initials: E
authors.lastname: Gobin
authors.forename: Pascale
authors.initials: P
authors.lastname: Marcorelles
authors.forename: Dale J
authors.initials: DJ
authors.lastname: Benos
authors.forename: Jack R
authors.initials: JR
authors.lastname: Lancaster
authors.forename: G Yancey
authors.initials: GY
authors.lastname: Gillespie
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D015687:Cell Hypoxia / Q000235:genetics; D004272:DNA, Mitochondrial / Q000502:physiology; D004734:Energy Metabolism / Q000187:drug effects / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D014411:Neoplastic Stem Cells / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012402:Rotenone / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D004272:DNA, Mitochondrial; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D014475:Uncoupling Agents; D012402:Rotenone