title: Gardenia jasminoides protects against cerulein-induced acute pancreatitis.
abstract: OBJECTIVE
  To investigate the effect of Gardenia jasminoides (GJ) on cerulein-induced acute pancreatitis (AP) in mice.
  
  
  METHODS
  C57BL/6 mice weighing 18-20 g were divided into three groups. (1) Normal saline-treated group, (2) treatment with GJ at a dose of 0.1 g/kg, (3) treatment with GJ at a dose of 1 g/kg. GJ was administered orally (n = 6 per group) for 1 wk. Three hours later, the mice were given an intraperitoneal injection of cerulein (50 microg/kg), a stable cholecystokinin (CCK) analogue, every hour for a total of 6 h as described previously. The mice were sacrificed at 6 h after completion of cerulein injections. Blood samples were obtained to determine serum amylase, lipase and cytokine levels. The pancreas was rapidly removed for morphologic examination and scoring. A portion of pancreas was stored at -70 degree and prepared for the measurement of tissue myeloperoxidase (MPO) activity, an indicator of neutrophil sequestration, and for reverse-transcriptase PCR (RT-PCR) and real-time PCR measurements.
  
  
  RESULTS
  Treatment with GJ decreased significantly the severity of pancreatitis and pancreatitis-associated lung injury. Treatment with GJ attenuated the severity of AP compared with saline-treated mice, as shown by reduction in pancreatic edema, neutrophil infiltration, serum amylase and lipase levels, serum cytokine levels, and mRNA expression of multiple inflammatory mediators.
  
  
  CONCLUSIONS
  These results suggest that GJ attenuated the severity of AP as well as pancreatitis-associated lung injury.
authors.affiliation: Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, Jeonju, 570-749 Jeonbuk, South Korea.
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Jung
authors.forename: Young-Seok
authors.initials: YS
authors.lastname: Chae
authors.forename: Do-Yun
authors.initials: DY
authors.lastname: Kim
authors.forename: Sang-Wan
authors.initials: SW
authors.lastname: Seo
authors.forename: Hee-Je
authors.initials: HJ
authors.lastname: Park
authors.forename: Gi-Sang
authors.initials: GS
authors.lastname: Bae
authors.forename: Tae-Hyeon
authors.initials: TH
authors.lastname: Kim
authors.forename: Hyo-Jeong
authors.initials: HJ
authors.lastname: Oh
authors.forename: Ki-Jung
authors.initials: KJ
authors.lastname: Yun
authors.forename: Rae-Kil
authors.initials: RK
authors.lastname: Park
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Kim
authors.forename: Eun-Cheol
authors.initials: EC
authors.lastname: Kim
authors.forename: Sung-Yeon
authors.initials: SY
authors.lastname: Hwang
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Park
authors.forename: Ho-Joon
authors.initials: HJ
authors.lastname: Song
mesh_terms: D000208:Acute Disease; D000284:Administration, Oral; D000681:Amylases / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001835:Body Weight; D002108:Ceruletide; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D032062:Gardenia; D018836:Inflammation Mediators / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008049:Lipase / Q000097:blood; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D055370:Lung Injury / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000187:drug effects; D009929:Organ Size; D010179:Pancreas / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D010936:Plant Extracts; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D002108:Ceruletide; D009195:Peroxidase; D008049:Lipase; D000681:Amylases