title: Calcium phosphate surface treatment of bioactive glass causes a delay in early osteogenic differentiation of adipose stem cells.
abstract: Human adipose stem cells (ASCs) combined with osteostimulative material provide an attractive approach for clinical bone regeneration. The effect of calcium phosphate (Ca-P) surface treatment of three-dimensional bioactive glass scaffolds on the attachment, proliferation, and osteogenic differentiation of ASCs was studied. Three types of bioactive glass scaffolds (nontreated, thick and thin Ca-P treated) were compared. All scaffold types supported ASC attachment, spreading, and proliferation equally as detected by scanning electron microscopy, fluorescence staining, and DNA measurement. Indices of osteogenic differentiation including the expression of osteopontin and alkaline phosphatase (ALP) were consistently higher in the nontreated and thin Ca-P-treated scaffolds when compared with thick Ca-P-treated scaffolds at 2 weeks. ASCs cultured on nontreated bioactive glass scaffolds showed significantly higher ALP activity when compared with both thin and thick Ca-P-treated scaffolds after 1 week in culture, but these differences equalized between the three scaffolds by the 2-week time point. In conclusion, osteogenic differentiation appears to be delayed on the Ca-P surface-treated scaffolds. This delay is more pronounced with thick Ca-P treatment of the scaffolds.
authors.affiliation: Regea Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Tampere, Finland. suvi.haimi@regea.fi
authors.forename: Suvi
authors.initials: S
authors.lastname: Haimi
authors.forename: Loredana
authors.initials: L
authors.lastname: Moimas
authors.forename: Eija
authors.initials: E
authors.lastname: Pirhonen
authors.forename: Bettina
authors.initials: B
authors.lastname: Lindroos
authors.forename: Heini
authors.initials: H
authors.lastname: Huhtala
authors.forename: Sari
authors.initials: S
authors.lastname: Räty
authors.forename: Hannu
authors.initials: H
authors.lastname: Kuokkanen
authors.forename: George K
authors.initials: GK
authors.lastname: Sándor
authors.forename: Susanna
authors.initials: S
authors.lastname: Miettinen
authors.forename: Riitta
authors.initials: R
authors.lastname: Suuronen
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D006801:Humans; D008875:Middle Aged; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; C022107:Bioglass; D002130:Calcium Phosphates; C020243:calcium phosphate