title: The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.
abstract: Biochemical assay of proteomic libraries derived from the Saccharomyces cerevisiae genome provides a powerful new tool for the assignment of activities to proteins. Particular advantages of this approach include the speed with which a protein can be identified and the generality for any biological activity for which an assay can be developed. We discuss the utility of this approach for the identification of RNA-modifying enzymes using a yeast proteomic library derived from a genomic set of strains expressing GST-ORF fusion proteins. This technique is also broadly applicable to other classes of RNA-protein interactions, including RNA binding and RNA degradation, and can be used with any of the proteomic libraries that are available.
authors.affiliation: Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York, USA.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Jackman
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Grayhack
authors.forename: Eric M
authors.initials: EM
authors.lastname: Phizicky
mesh_terms: D011485:Protein Binding; D040901:Proteomics / Q000379:methods*; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D016601:RNA-Binding Proteins; D012313:RNA