Spectrofluorimetric determination of trace nitrite in food products with a new fluorescent probe 1,3,5,7-tetramethyl-2,6-dicarbethoxy-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene.
A new fluorescent probe 1,3,5,7-tetramethyl-2,6-dicarbethoxy-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene (TMDCDABODIPY) has been developed to detect nitrite in meat products and vegetables. The fluorescence of TMDCDABODIPY is very weak, but when it reacts with nitrite, a strong fluorescent triazole forms in aqueous medium at room temperature, which offers the advantage of specificity and sensitivity for the determination of nitrite. The fluorescence intensity was linear over a nitrite concentration of 9-300 nmol l(-1) with a detection limit of 0.21 nmol l(-1) (S/N=3). The proposed method has been used for the determination of trace nitrite in food products with the recoveries of 94.62-105.48%.
Huang
Ke-Jing
KJ
Department of Chemistry, Wuhan University, Wuhan, China.
Wang
Hong
H
Guo
Yue-Hong
YH
Fan
Rong-Li
RL
Zhang
Hua-Shan
HS