A dynamic assessment of sperm DNA fragmentation versus sperm viability in proven fertile human donors.
OBJECTIVE
To analyze any possible dynamic correlation between sperm DNA fragmentation and sperm viability.


METHODS
The rate of viability loss and the rate of increase of the frequency of sperm cells with fragmented DNA were determined at 0, 1.5, 4.5, and 24.0 hours after thawing samples from donors with proven fertility.


METHODS
Academic biology and reproductive medicine centers.


METHODS
Fifteen male donors with proven fertility for a maximum of six births at the reproductive medicine center.


METHODS
None.


METHODS
Sperm DNA fragmentation and viability dynamics expressed as logarithmic coefficients of change.


RESULTS
The dynamics of sperm DNA fragmentation and sperm viability adjusted to a logarithmic function with an initial highest velocity that progressively decreases. Nevertheless, the rates were not statistically significantly correlated.


CONCLUSIONS
In the short term, dynamic dysfunction of membrane permeability does not result in DNA fragmentation and thus must be considered as independent parameters of sperm quality.
Gosálvez
Jaime
J
Genetics Unit, Department of Biology, Universidad Autónoma de Madrid (UAM), Madrid, Spain. jaime.gosalvez@uam.es
Cortés-Gutiérrez
Elva I
EI
Nuñez
Rocio
R
Fernández
José Luís
JL
Caballero
Pedro
P
López-Fernández
Carmen
C
Holt
William V
WV
D002470:Cell Survival; D053938:DNA Fragmentation; D005260:Female; D005298:Fertility; D006801:Humans; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D027724:Reproductive Techniques, Assisted; D013074:Sperm Banks / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology; D014019:Tissue Donors