Label-free fluorescence detection in capillary and microchip electrophoresis.
Herein, we summarize the current status of native fluorescence detection in microchannel electrophoresis, with a strong focus on chip-based systems. Fluorescence detection is a powerful technique with unsurpassed sensitivity down to the single-molecule level. Accordingly fluorescence detection is attractive in combination with miniaturised separation techniques. A drawback is, however, the need to derivatize most analytes prior to analysis. This can often be circumvented by utilising excitation light in the UV spectral range in order to excite intrinsic fluorescence. As sensitive absorbance detection is challenging in chip-based systems, deep-UV fluorescence detection is currently one of the most general optical detection techniques in microchip electrophoresis, which is especially attractive for the detection of unlabelled proteins. This review gives an overview of research on native fluorescence detection in capillary (CE) and microchip electrophoresis (MCE) between 1998 and 2008. It discusses material aspects of native fluorescence detection and the instrumentation used, with particular focus on the detector design. Newer developments, featured techniques, and their prospects in the future are also included. In the last section, applications in bioanalysis, drug determination, and environmental analysis are reviewed with regard to limits of detection.
Schulze
Philipp
P
Institute of Analytical Chemistry, Universitat Leipzig, Johannisallee 29, 04103, Leipzig, Germany.
Belder
Detlev
D
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D005453:Fluorescence; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012680:Sensitivity and Specificity
D004785:Environmental Pollutants; D004364:Pharmaceutical Preparations