title: Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip.
abstract: A glass electrophoresis microchip integrated a flow-type chemiluminescence (CL) detection cell has been developed and evaluated. The chip pattern is a double-T-type electrophoretic sample injection and separation combining with a Y-type chemiluminecent detector. The double-T geometry allows for high-efficiency sample injection and geometric definition of sample plug size. The branch of Y was used as CL reagent channel, and the CL reagent was delivered by a lab-made micropump. Bis[(2,4,6-trichlorophenyl)]oxalate-H(2)O(2) CL system was employed to detect dansyl amino acids. On this microchip, dansyl-phenylalanine and -sarcosine were successfully separated by electrophoresis and detected within 250s. The detection limits (S/N=3) of dansyl-phenylalanine and -sarcosine could reach to 2.8 and 3.2muM, respectively, due to the vigorous dilution of sample with CL reagent and timely removal of the waste solution from reaction area.
authors.affiliation: Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
authors.forename: Rongguo
authors.initials: R
authors.lastname: Su
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
authors.forename: Katsumi
authors.initials: K
authors.lastname: Uchiyama
authors.forename: Masaaki
authors.initials: M
authors.lastname: Yamada