title: Enhanced chemiluminescence determination of alpha-amylase by use of amylose labelled with horseradish peroxidase.
abstract: An enhanced chemiluminescence method for determining soluble and immobilized alpha-amylase has been developed, based on use of an insoluble amylose substrate labelled with horseradish peroxidase. Soluble peroxidase-labelled fragments of the substrate, released by the action of alpha-amylase, are quantified by the peroxidase-catalyzed luminol-peroxide-p-iodophenol reaction. The detection limit for alpha-amylase was 125 fmole (12.5 nM). An insoluble amylopectin labelled with horseradish peroxidase was also effective as a substrate for this type of assay.
authors.affiliation: Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, PA 19104, U.S.A.
authors.forename: L J
authors.initials: LJ
authors.lastname: Kricka
authors.forename: J M
authors.initials: JM
authors.lastname: Marcinkowski
authors.forename: P
authors.initials: P
authors.lastname: Wilding
authors.forename: S
authors.initials: S
authors.lastname: Lekhakula