Molecular characterization and expression analysis of a new cDNA encoding strictosidine synthase from Ophiorrhiza japonica.
A new full-length cDNA encoding strictosidine synthase (designated as OjSTR, GenBank Accession No. 1087598), which catalyzes a committed step in camptothecin biosynthetic pathway, was isolated from young leaves of Ophiorrhiza japonica for the first time. OjSTR was 1,258 bp and contained a 1,062 bp open reading frame encoding a deduced protein of 353 amino acid residues. Sequence analyses showed that OjSTR had high homology with other STRs from some TIA-producing plants. Phylogenetic tree analysis showed that OjSTR had closest relationship with STR from O. pumila. Tissue expression pattern analysis revealed that OjSTR constitutively expressed in all the tested tissues at different levels, which was high in flower, moderate in leaf and root, low in stem. Expression profiles under plant defense signals such as methyl jasmonate and salicylic acid were investigated, and the results revealed that expression of OjSTR was all induced, implying that OjSTR was high elicitor responsive.
Lu
Yang
Y
Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
Wang
Hongshen
H
Wang
Wei
W
Qian
Zhongying
Z
Li
Li
L
Wang
Jing
J
Zhou
Genyu
G
Kai
Guoyin
G
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D019759:Carbon-Nitrogen Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D019295:Computational Biology; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics*; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D017433:Protein Structure, Secondary; D019666:Rubiaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D020156:Salicylic Acid / Q000494:pharmacology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014748:Vinca Alkaloids / Q000737:chemistry
D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D014748:Vinca Alkaloids; C021093:strictosidine; C072239:methyl jasmonate; D019759:Carbon-Nitrogen Lyases; C022684:strictosidine synthetase; D020156:Salicylic Acid