Protein adsorption on derivatives of hyaluronic acid and subsequent cellular response.
The modulation of biological interactions with artificial surfaces is a vital aspect of biomaterials research. Serum protein adsorption onto photoreactive hyaluronic acid (Hyal-N(3)) and its sulfated derivative (HyalS-N(3)) was analyzed to determine extent of protein interaction and protein conformation as well as subsequent cell adhesion. There were no significant (p < 0.01) differences in the amount of protein adsorbed to the two polymers; however, proteins were found to be more loosely bound on HyalS-N(3) compared with Hyal-N(3). Fibronectin was adsorbed onto HyalS-N(3) in such an orientation as to allow the availability of the cell binding region, while there was more restricted access to this region on fibronectin adsorbed onto Hyal-N(3). This was confirmed by reduced cell adhesion on fibronectin precoated Hyal-N(3) compared with fibronectin precoated HyalS-N(3). Minimal cell adhesion was observed on albumin and serum precoated Hyal-N(3). The quartz crystal microbalance confirmed that specific cell-surface interactions were experienced by cells interacting with the fibronectin precoated polymers and serum precoated HyalS-N(3).
Lord
Megan S
MS
Graduate School of Biomedical Engineering, The University of New South Wales, Sydney, New South Wales 2052, Australia. m.lord@unsw.edu.au
Pasqui
Daniela
D
Barbucci
Rolando
R
Milthorpe
Bruce K
BK
D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005353:Fibronectins / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D010777:Photochemistry / Q000379:methods; D011108:Polymers / Q000737:chemistry; D012867:Skin / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013582:Synovial Fluid / Q000378:metabolism
D004337:Drug Carriers; D005353:Fibronectins; D011108:Polymers; D006820:Hyaluronic Acid