Reflectometric cutinase assay for rapid screening of contaminants and residues of insecticidal organophosphates and carbamates.
Because of the extensive use of insecticides in agriculture, there is an increasing demand for rapid analytical methods for residues in food and feed control. To meet this need, a completely new application of the reflectometric lipase test (Reflectoquant, Merck) was developed. By using the cutinase-induced reaction of the substrate 5-bromo-4-chloro-3-indoxyl caprylate on the test strips, residues of organophosphates and carbamates can be determined on the basis of enzyme inhibition in a fast and inexpensive way. With this technique, we investigated the inhibition effects of representative insecticides, i.e., chlorpyrifos oxon, paraoxon, and carbaryl. The bimolecular inhibitory rate constants (ki) were found to agree well with those obtained by a previously described spectrophotometric cutinase assay in the microtiter-plate format. Recoveries determined with the strip test from spiked samples compared well with those obtained by both the cutinase microtiter-plate assay and liquid chromatographylmass spectrometry.
Walz
Ingrid
I
Universität Hohenheim, Institut für Lebensmittelchemie, Garbenstrasse 28, D-70599 Stuttgart, Germany.
Schwack
Wolfgang
W
D002219:Carbamates / Q000032:analysis*; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents; D005670:Fusarium / Q000201:enzymology; D007202:Indicators and Reagents; D007203:Indigo Carmine; D007306:Insecticides / Q000032:analysis*; D013058:Mass Spectrometry; D000432:Methanol; D009943:Organophosphorus Compounds / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D012996:Solutions; D012997:Solvents
D002219:Carbamates; D004396:Coloring Agents; D007202:Indicators and Reagents; D007306:Insecticides; D009943:Organophosphorus Compounds; D010573:Pesticide Residues; D012996:Solutions; D012997:Solvents; D007203:Indigo Carmine; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D000432:Methanol