title: Profiling and imaging of lipids on brain and liver tissue by matrix-assisted laser desorption/ ionization mass spectrometry using 2-mercaptobenzothiazole as a matrix.
abstract: 2-Mercaptobenzothiazole (MBT) is employed for the first time as a matrix for the analysis of lipids from tissue extracts using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. We demonstrate that the performance of MBT is superior to that of the matrixes commonly employed for lipids, due to its low vapor pressure, its low acidity, and the formation of small crystals, although because of the strong background at low m/z, it precludes detection of species below approximately 500 Da. This inconvenience can be partly overcome with the formation of Cs adducts. Using a polymer-based dual calibration, a mass accuracy of approximately 10 ppm in lipid extracts and of approximately 80 ppm in tissues is achieved. We present spectra from liver and brain lipid extracts where a large amount of lipid species is identified, in both positive and negative ion modes, with high reproducibility. In addition, the above-mentioned special properties of MBT allow its employment for imaging mass spectrometry. In the present work, images of brain and liver tissues showing different lipid species are presented, demonstrating the advantages of the employment of MBT.
authors.affiliation: Department of Chemical Physics, Faculty of Science and Technology, University of the Basque Country, B(o) Sarriena s/n, 48940 Leioa, Spain.
authors.forename: Egoitz
authors.initials: E
authors.lastname: Astigarraga
authors.forename: Gabriel
authors.initials: G
authors.lastname: Barreda-Gómez
authors.forename: Laura
authors.initials: L
authors.lastname: Lombardero
authors.forename: Olatz
authors.initials: O
authors.lastname: Fresnedo
authors.forename: Fernando
authors.initials: F
authors.lastname: Castaño
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Giralt
authors.forename: Begoña
authors.initials: B
authors.lastname: Ochoa
authors.forename: Rafael
authors.initials: R
authors.lastname: Rodríguez-Puertas
authors.forename: José A
authors.initials: JA
authors.lastname: Fernández
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D001923:Brain Chemistry; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D008099:Liver / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D052160:Benzothiazoles; D008055:Lipids; C005563:captax