Influence of nanofibers on the growth and osteogenic differentiation of stem cells: a comparison of biological collagen nanofibers and synthetic PLLA fibers.
The aim of this study was to compare biological collagen I (ColI) and synthetic poly-(L: -lactide) (PLLA) nanofibers concerning their stability and ability to promote growth and osteogenic differentiation of human mesenchymal stem cells in vitro. Matrices were seeded with human stem cells and cultivated over a period of 28 days under growth and osteoinductive conditions and analyzed during the course. During this time the PLLA nanofibers remained stable while the presence of cells resulted in an attenuation of the ColI nanofiber mesh. Although there was a tendency for better growth and osteoprotegerin production of stem cells when cultured on collagen nanofibers, there was no significant difference compared to PLLA nanofibers or controls. The gene expression of alkaline phosphate, osteocalcin and collagen I diminished in the initial phase of cultivation independent of the polymer used. In the case of PLLA fibers, this gene expression normalized during the course of cultivation, whereas the presence of collagen nanofibers resulted in an increased gene expression of osteocalcin and collagen during the course of the experiment. Taken together the PLLA fibers were easier to produce, more stable and did not compromise growth and differentiation of stem cells over the course of experiment. On the other hand, collagen nanofibers supported the differentiation process to some extent. Nevertheless, the need for fixation as well as the missing stability during cell culture requires further work.
Schofer
Markus Dietmar
MD
Department of Orthopedics, University of Marburg, Baldingerstrasse, 35043, Marburg, Germany. schofer@med.uni-marburg.de
Boudriot
Ulrich
U
Wack
Christina
C
Leifeld
Irini
I
Gräbedünkel
Christian
C
Dersch
Roland
R
Rudisile
Markus
M
Wendorff
Joachim Heinz
JH
Greiner
Andreas
A
Paletta
Jürgen Rudolf Josef
JR
Fuchs-Winkelmann
Susanne
S
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001672:Biocompatible Materials; D002452:Cell Count; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis; D053244:Osteoprotegerin / Q000378:metabolism; D011091:Polyesters; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
D001672:Biocompatible Materials; D024042:Collagen Type I; D053244:Osteoprotegerin; D011091:Polyesters; C506030:TNFRSF11B protein, human; D015675:Osteocalcin; C033616:poly(lactide); D000469:Alkaline Phosphatase