title: Inhibition of cell proliferation and invasion in a human colon cancer cell line by 5-aminosalicylic acid.
abstract: BACKGROUND
  5-Aminosalicylic acid lacks the well-known side effects associated with the long-term use of non-steroidal anti-inflammatory drugs. We investigated anti-carcinogenic mechanisms of 5-aminosalicylic acid on a colon cancer cell line.
  
  
  METHODS
  MTT analysis was performed for various colon cancer cell lines. The expression of NF-kappaB and metalloproteinases was examined in either HT-29 cells treated with IL-1beta and/or 5-aminosalicylic acid. Matrigel assay was used to evaluate invasive potential of HT-29 cells. Analysis of a cDNA microarray containing 8700 genes was performed to identify the alteration of gene expression in response to treatment to 5-aminosalicylic acid.
  
  
  RESULTS
  The use of MTT analysis showed that 5-aminosalicylic acid suppressed the growth of HT-29 cells. The activity of NF-kappaB was also decreased by combined-treatment with IL-1beta and 5-aminosalicylic acid. The use of an ELISA and zymography demonstrated that MMP-2 and MMP-9 enzyme activity were decreased in HT-29 cells by treatment with various concentration of 5-aminosalicylic acid. A matrigel analysis demonstrated that 5-aminosalicylic acid treatment on HT-29 significantly inhibited the invasiveness of the cells. In cDNA microarray, 163 genes following 5-aminosalicylic acid exposure showed altered expression.
  
  
  CONCLUSIONS
  This study indicated that 5-aminosalicylic acid suppresses the growth of human colon cancer cells and is able to inhibit MMPs expression via NF-kappaB mediated cell signals and invasiveness.
authors.affiliation: Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Y-H
authors.initials: YH
authors.lastname: Kim
authors.forename: M H
authors.initials: MH
authors.lastname: Kim
authors.forename: B J
authors.initials: BJ
authors.lastname: Kim
authors.forename: J J
authors.initials: JJ
authors.lastname: Kim
authors.forename: D K
authors.initials: DK
authors.lastname: Chang
authors.forename: H J
authors.initials: HJ
authors.lastname: Son
authors.forename: P-L
authors.initials: PL
authors.lastname: Rhee
authors.forename: J C
authors.initials: JC
authors.lastname: Rhee
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology* / Q000517:prevention & control*; D015536:Down-Regulation / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D019804:Mesalamine / Q000008:administration & dosage*; D045726:Metalloproteases / Q000187:drug effects / Q000235:genetics; D016328:NF-kappa B / Q000187:drug effects; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016328:NF-kappa B; D019804:Mesalamine; D045726:Metalloproteases