title: Molecular characterization of nosocomial CTX-M type beta-lactamase producing Enterobacteriaceae from a tertiary care hospital in south India.
abstract: CTX-M group of extended spectrum beta lactamases (ESBLs) represents a rapidly emerging problem in many countries. The prevalence of nosocomial bla CTX-M-1 producing Enterobacteriaceae strains has not been reported earlier in Indian hospitals. This study describes molecular subtyping of nosocomial bla CTX-M producing strains of Enterobacteriaceae . Polymerase chain reaction with primers specific for bla CTX-M-1 coding genes was used to identify 95 Enterobacteriaceae strains producing bla CTX-M positive isolates. Of the 95 bla CTX-M producing isolates, 45 strains were positive for bla CTX-M-1 . bla CTX-M-1 was found to be most prevalent in Klebsiella strains.
authors.affiliation: Department of Microbiology, International Centre for Cardio Thoracic and Vascular Diseases, A Unit of Frontier Lifeline, R-30-C, Ambattur Industrial Estate Road, Mogappair, Chennai, Tamilnadu, India. sam_jemi@yahoo.co.in
authors.forename: S A
authors.initials: SA
authors.lastname: Jemima
authors.forename: S
authors.initials: S
authors.lastname: Verghese
mesh_terms: D003227:Conjugation, Genetic; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D007194:India / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D015995:Prevalence; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C055184:beta-lactamase TEM-3; D001618:beta-Lactamases