title: Fluorescence wavelength, intensity and lifetime for multidimensional transduction of selective interactions of acetylcholine receptor by lipid membranes.
abstract: Concurrent analysis of the fluorescence intensity, at different emission wavelengths, of lipid vesicles containing acetylcholine receptor (AChR) labelled with a nitrobenzoxadiazole (NBD) moiety shows that selective interactions with the agonist carbamylcholine can be detected reproducibly by a self-calibration method with muM detection limits. Concurrent analysis of the fluorescence intensity and lifetime of the new probe 4-dicyanomethylene-1,2,3,4-tetrahydromethylquinoline (DCQ) shows that general alterations of lipid membrane structure induced by temperature variation in the head-group region of lipid vesicles can be determined. A general approach to detection of selective interactions is introduced by observation of fluorescence intensity and lifetime changes of the probe NBD-phosphatidyl ethanolamine dispersed in lipid membranes containing unlabelled AChR. Detection and differentiation of selective interactions between carbamylcholine and the antagonist alpha-bungarotoxin are possible by correlation with intensity and lifetime at different emission wavelengths.
authors.affiliation: Chemical Sensors Group, Department of Chemistry, Erindale Campus, University of Toronto, 3359 Mississauga Road North, Mississauga, Ontario, Canada L5L 1C6.
authors.forename: U J
authors.initials: UJ
authors.lastname: Krull
authors.forename: R S
authors.initials: RS
authors.lastname: Brown
authors.forename: B D
authors.initials: BD
authors.lastname: Hougham
authors.forename: G
authors.initials: G
authors.lastname: McGibbon
authors.forename: E T
authors.initials: ET
authors.lastname: Vandenberg