title: Immune response of gnotobiotic piglets against Mycoplasma hyopneumoniae.
abstract: In this study, several cytokine responses were investigated during Mycoplasma hyopneumoniae (Mhp) infection using a gnotobiotic infection model. We found that several inflammatory cytokines (IL-1beta, IL-8, IL-18, and TNF-alpha) and an anti-inflammatory cytokine IL-10 were induced from peripheral blood mononuclear cells (PBMC) of germ-free (GF) piglets stimulated with heat killed Mhp whole antigens, but no IFN-gamma and IL-4 were induced by Mhp. After the intranasal infection of Mhp, IL-1beta, IL-8, IL-18, and IFN-gamma were also detected in the broncho-alveolar lavage fluids (BALF). The antigen-specific IFN-gamma and IL-10 responses after infection of Mhp were gradually suppressed during Mhp infection as well as non-specific immune response to concanavalin A (ConA) and lipopolysacchalide (LPS) at early stage of infection. These results suggested that Mhp infection modulates the immune response of pigs by inducing several cytokines, and causes immuno-suppression of pigs in a gnotobiotic condition.
authors.affiliation: Research Team for Advanced Biologicals, National Institute of Animal Health, Tsukuba, Ibaraki, Japan. ymuneta@affrc.go.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Muneta
authors.forename: Yu
authors.initials: Y
authors.lastname: Minagawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimoji
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hikono
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Mori
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000097:blood; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology / Q000382:microbiology; D003208:Concanavalin A / Q000276:immunology; D016207:Cytokines / Q000097:blood / Q000276:immunology; D005856:Germ-Free Life / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D012140:Respiratory Tract Diseases / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D016207:Cytokines; D008070:Lipopolysaccharides; D003208:Concanavalin A