title: Dynamic NanoSIMS ion imaging of unicellular freshwater algae exposed to copper.
abstract: This work demonstrates the capabilities of nanoscale secondary-ion mass spectrometry, using the Cameca NanoSIMS50 ion microprobe, to detect and image the copper-ion distribution in microalgal cells exposed to nanomolar and micromolar copper concentrations. In parallel to (63)Cu(-) secondary-ion maps, images of (12)C(-), (12)C(14)N(-), and (31)P(-) secondary ions were collected and analysed. A correlation of (63)Cu(-) secondary-ion maps with those found for (12)C(14)N(-) and (31)P(-) demonstrated the possible association of Cu with cell components rich in proteins and phosphorus. The results highlighted the potential of NanoSIMS for intracellular tracking of essential trace elements such as Cu in single cells of the microalga Chlorella kesslerii.
authors.affiliation: Environmental Biophysical Chemistry, Environmental Science and Technology Institute, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne, Station 2, 1015, Lausanne, Switzerland. vera.slaveykova@epfl.ch
authors.forename: Vera I
authors.initials: VI
authors.lastname: Slaveykova
authors.forename: Cédric
authors.initials: C
authors.lastname: Guignard
authors.forename: Tanja
authors.initials: T
authors.lastname: Eybe
authors.forename: Henri-Noël
authors.initials: HN
authors.lastname: Migeon
authors.forename: Lucien
authors.initials: L
authors.lastname: Hoffmann
mesh_terms: D000460:Chlorophyta / Q000737:chemistry* / Q000187:drug effects* / Q000254:growth & development; D003300:Copper / Q000032:analysis* / Q000494:pharmacology*; D005618:Fresh Water; D007477:Ions / Q000032:analysis; D036103:Nanotechnology; D012680:Sensitivity and Specificity; D018629:Spectrometry, Mass, Secondary Ion
chemical_list: D007477:Ions; D003300:Copper