[Study of the elements determination method in animal fur by microwave digestion ICP-AES].
Considering the complex matrix of the sample, the animal fur is carried on to the sample pretreatment method studies specially. The microwave closed system has its unique merit: The microwave radiation has the very strong penetrability and the rapid in-depth heating function. After absorbing microwave the sample and the molecules of reactant may carry on the reaction in short time. But the microwave power is very weak, reaction consumes much time, the resolution is also incomplete. Besides the output excessively is high dispels in the pot the reagent differential pressure to increase the test solution to produce the storm rapidly to boil. As a result of those flaws, the minute step microwave heating digestion method is used to digest test specimen after treated by the acid pickling over night. In the experiment, the specialized microwave reactor is replaced by civil microwave; the microwave heating technology is adopted. According to the different characteristics of reagents, different allocated proportion and the test solution volume of nitric acid, hydrogen peroxide, hydrochloric acid and the water are tested separately. Meanwhile, in order to optimize the experimental condition, the different response power and respond time is also studied. At last, the experimental condition is determined: HNO3-H2O2-HCl-H2O acid system is chose(four reagent allocated proportions are 8:1:1:5); test specimen is heated up 10 minutes when the output is 150 W and 5 minutes when the output is 360 W continuously; carries on the test specimen airtight resolution processing animal fur by the sample. To guarantee the standard solution system is consistent with the biological sample substrate, the artificial simulation biology sample substrate is used to match law configuration standard solution; the ration the substrate element calcium is added. To eliminate disturbance of the sample complex substrate, the substrate match law, which reduces the substrate element disturbance is used. Inductive coupling plasma atom emission spectral analysis technology is also adopted in the experiment. This method has several advantages: the rapidity of the examination, the scope width of the examination, low consumption of test specimens and low limitation of picking out. This method is able to simultaneously examine the aluminum, the iron, the calcium, the magnesium, the boron, the barium, the cadmium, the cobalt, the copper, the chromium, the manganese, the molybdenum, the nickel, the phosphorus, the lead, the strontium, the zinc, the titanium, 18 kind of constants and trace element existed in the animal fur. There kinds of animal furs were examined. Basically, the constants of calcium were close. The content of magnesium and strontium in the furs of the herbivorous were higher than the omnivorous and the carnivorous, while the content of phosphorus in the furs of the herbivorous was lower than the other tow, so did the trace element, namely aluminum, iron, copper, zinc. It was obvious that only in the fur of the carnivorous was the content trace element titanium included. Based on the examination result, we could draw the conclusion that contain connection, which may explain the intrinsic and the external relationship concerning the animal, exists between the animal habit and the element content in their body. This method sends the standard material GBW07601 confirmation after the national person, obtains the method relative error in between 0.83%-9.59%, the relatively standard deviation between 0.81%-5.20%. The limitation of picking out, the accuracy and the accuracy each examination target is obtained in the animal fur by the actual sample analysis in the confirmation, which can satisfy the biological sample examination request.
Hou
Tian-ping
TP
Jilin Teachers Institute of Engineeing and Technology, Changchun 130052, China.
Wang
Song-jun
SJ
Cao
Lin
L
Chang
Ping
P
Hou
Yue
Y
D000818:Animals; D006197:Hair / Q000737:chemistry*; D008872:Microwaves; D013054:Spectrophotometry, Atomic; D014131:Trace Elements / Q000032:analysis* / Q000737:chemistry
D014131:Trace Elements