A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses.
BACKGROUND
Three new human polyomaviruses have been recently described, and investigating their in vivo biology and pathogenicity will require sensitive and rational detection assays.


OBJECTIVE
To develop and evaluate a sensitive and rational assay for detection of the newly identified KI and WU polyomaviruses.


METHODS
A single-tube, dual-probe, real-time PCR assay for simultaneous detection and discrimination of KI and WU polyomaviruses was developed.


RESULTS
The assay had near single-molecule sensitivity for both viruses and no cross-reactivity was observed. A panel of 637 nasopharyngeal aspirates was screened, resulting in a frequency of 1.4% for KIPyV and 1.3% for WUPyV.


CONCLUSIONS
The dual-probe assay provides a rational approach for further studies of KIPyV and WUPyV.
Lindau
Cecilia
C
Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
Tiveljung-Lindell
Annika
A
Goh
Shan
S
Ramqvist
Torbjörn
T
Allander
Tobias
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D009305:Nasopharynx / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011120:Polyomavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000821:virology; D055815:Young Adult