Organotypic culture of adult rabbit retina.
Organotypic culture systems of functional neural tissues are important tools in neurobiological research. Ideally, such a system should be compatible with imaging techniques, genetic manipulation, and electrophysiological recording. Here we present a simple interphase tissue culture system for adult rabbit retina that requires no specialized equipment and very little maintenance. We demonstrate the dissection and incubation of rabbit retina and particle-mediated gene transfer of plasmids encoding GFP or a variety of subcellular markers into retinal ganglion cells. Rabbit retinas cultured this way can be kept alive for up to 6 days with very little changes of the overall anatomical structure or the morphology of individual ganglion- and amacrine cells.
Lye
Ming H
MH
Massachusetts General Hospital, Harvard Medical School, USA.
Jakobs
Tatjana C
TC
Masland
Richard H
RH
Koizumi
Amane
A
D000367:Age Factors; D000818:Animals; D018014:Gene Transfer Techniques; D042282:Microdissection / Q000379:methods*; D009924:Organ Culture Techniques / Q000379:methods*; D011817:Rabbits; D012160:Retina / Q000502:physiology*