title: Inhibition of acrolein-stimulated MUC5AC production by fucoidan in human bronchial epithelial cells.
abstract: Fucoidan, a marine sulfated polysaccharide has both antithrombotic and anti-inflammatory effects. We determined the effect of fucoidan on MUC5AC expression in a human bronchial epithelial cell line, NCI-H292. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that fucoidan inhibited MUC5AC expression and protein secretion in cells stimulated with acrolein, a toxic aldehyde present in tobacco smoke. The activation of both nuclear factor-kappa B (NF-kappa B) and activator protein 1 (AP-1) are key steps in the transcriptional activation of MUC5AC. We found that the acrolein-mediated transactivation of MUC5AC was selectively dependent on AP-1 activation and was suppressed by fucoidan. Fucoidan-induced AP-1 inhibition and MUC5AC repression might be associated with fucoidan's protective effects against respiratory diseases.
authors.affiliation: BK21 Project Team, College of Pharmacy, Chosun University, Gwangju, South Korea.
authors.forename: Yuba Raj
authors.initials: YR
authors.lastname: Pokharel
authors.forename: Se Young
authors.initials: SY
authors.lastname: Yoon
authors.forename: Sang Kyum
authors.initials: SK
authors.lastname: Kim
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Li
authors.forename: Keon Wook
authors.initials: KW
authors.lastname: Kang
mesh_terms: D000171:Acrolein / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D055271:Mucin 5AC / Q000096:biosynthesis*; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D016328:NF-kappa B; D011134:Polysaccharides; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D000171:Acrolein; C007789:fucoidan