title: Computer-assisted optimization of HPLC with post-column reaction for the determination of amino-acids.
abstract: Computer-assisted optimization of a high-performance liquid chromatograph and associated post-column reactor is reported for the determination of six amino-acids. First six, then seven, experimental variables were considered. Non-standard experimental conditions were found which gave significantly improved colour development in the ninhydrin reaction. The composite modified simplex method for experimental optimization and a novel system-response function facilitated rapid and simultaneous improvement of separation, sensitivity and analysis time. The approach described is directly applicable to many similar systems.
authors.affiliation: The Chemistry Department, University College Swansea, Singleton Park, Swansea, Wales U.K.
authors.forename: S
authors.initials: S
authors.lastname: Al-Najafi
authors.forename: C A
authors.initials: CA
authors.lastname: Wellington
authors.forename: A P
authors.initials: AP
authors.lastname: Wade
authors.forename: T J
authors.initials: TJ
authors.lastname: Sly
authors.forename: D
authors.initials: D
authors.lastname: Betteridge