Comparative proteomical analysis of zygotic embryo and endosperm from Coffea arabica seeds.
During coffee seed development, proteins are predominantly deposited in cotyledons and in the endosperm. Reserve proteins of the 11S family are the most abundant globulins in coffee seeds, acting as a nitrogen source during roasting and guaranteeing flavor and aroma. The aim of the present study was to compare the protein profiles of endosperm and zygotic embryos of coffee seeds. Proteins were extracted from whole seed as well as from embryo and endosperm, separately. Total proteins were analyzed by two-dimensional electrophoresis (2-DE) followed by identification by mass spectrometry (MS). The most abundant spots observed in the gels of coffee seeds were excised, digested with trypsin, and identified by MS as subunits of the 11S globulin. Spots with identical pI and molecular masses were also observed in the protein profiles of coffee endosperm and embryo, indicating that 11S protein is also highly expressed in those tissues. Peptide sequence coverage of about 20% of the entire 11S globulin was obtained. Three other proteins were identified in the embryo and endosperm 2-DE profiles as a Cupin superfamily protein, an allergenic protein (Pru ar 1), exclusive to the endosperm 2D map, and a hypothetical protein, observed only in the zygotic embryo profile.
Koshino
Lívia L
LL
Universidade de Brasília, Minas Gerais, Brazil.
Gomes
Clarissa P
CP
Silva
Luciano P
LP
Eira
Mirian T S
MT
Bloch
Carlos
C
Franco
Octávio L
OL
Mehta
Angela
A
D000595:Amino Acid Sequence; D040503:Coffea / Q000196:embryology*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry; D055314:Seed Storage Proteins / Q000032:analysis; D012639:Seeds / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism
D010940:Plant Proteins; D055314:Seed Storage Proteins; D014357:Trypsin