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Aromatic nitro compounds are reduced to aromatic amines by iron(II) in alkaline medium. The dye obtained by reacting the amine with 1,2-naphthoquinone-4-sulphonic acid is extractable into methylene chloride. Reduction by potassium borohydride yields a fluorescent o-diphenol, thus allowing the fluorimetric estimation of aromatic nitro compounds on the microgram scale.
Bartos
J
J
Centre de Recherches Roussel-Uclaf, 93-Romainville, France.