Protective effect of geranylgeranylacetone on cisplatin ototoxicity.
BACKGROUND
Geranylgeranylacetone (GGA) has the ability to induce heat shock proteins and to protect cells from apoptotic insults. This study aims to investigate whether GGA has a protective effect on cisplatin (CDDP) ototoxicity.


METHODS
The auditory threshold was assessed using the auditory brainstem response test. Hsp70 and C-reactive protein expressions were investigated by Western blot analysis. The amount of hair cells was counted under scanning electron microscopy.


RESULTS
The auditory threshold and the percentage of missing outer hair cells in the CDDP group were significantly higher than in the GGA + CDDP group. C-reactive protein expression was less in the GGA + CDDP group compared with the CDDP group. Hsp70 expression showed an adverse result.


CONCLUSIONS
It was suggested that GGA had a protective effect on CDDP ototoxicity.
Yin
Hai Ying
HY
Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Wen Hua Xi Road 107, Jinan, China.
Ma
Xiu Fang
XF
Liu
Fang
F
Xia
Ming
M
Xu
An Ting
AT
D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D002097:C-Reactive Protein / Q000187:drug effects / Q000378:metabolism; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D004224:Diterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D018840:HSP70 Heat-Shock Proteins / Q000187:drug effects / Q000378:metabolism; D006198:Hair Cells, Auditory / Q000187:drug effects*; D015151:Immunoblotting; D008855:Microscopy, Electron, Scanning
D000970:Antineoplastic Agents; D004224:Diterpenes; D018840:HSP70 Heat-Shock Proteins; D002097:C-Reactive Protein; D002945:Cisplatin; C031049:geranylgeranylacetone