Ex vivo mechanical loading of tendon.
Injuries to the tendon (e.g., wrist tendonitis, epicondyltis) due to overuse are common in sports activities and the workplace. Most are associated with repetitive, high force hand activities. The mechanisms of cellular and structural damage due to cyclical loading are not well known. The purpose of this video is to present a new system that can simultaneously load four tendons in tissue culture. The video describes the methods of sterile tissue harvest and how the tendons are loaded onto a clamping system that is subsequently immersed into media and maintained at 37 degrees C. One clamp is fixed while the other one is moved with a linear actuator. Tendon tensile force is monitored with a load cell in series with the mobile clamp. The actuators are controlled with a LabView program. The four tendons can be repetitively loaded with different patterns of loading, repetition rate, rate of loading, and duration. Loading can continue for a few minutes to 48 hours. At the end of loading, the tendons are removed and the mid-substance extracted for biochemical analyses. This system allows for the investigation of the effects of loading patterns on gene expression and structural changes in tendon. Ultimately, mechanisms of injury due to overuse can be studies with the findings applied to treatment and prevention.
Asundi
Krishna
K
Department of Bioengineering, University of California, Berkeley, USA.
Rempel
David
D
D000818:Animals; D015870:Gene Expression; D011817:Rabbits; D013710:Tendons / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors; D046509:Tissue Culture Techniques; D020858:Tissue and Organ Harvesting / Q000379:methods; D016474:Weight-Bearing