A new fluorogenic thiol-selective reagent: N-{p-[2-(6-dimethylamino)benzofuranyl]phenyl}maleimide.
N-{p-[2-(6-Dimethylamino)benzofuranyl]phenyl}maleimide (DBPM), has been synthesized and found to give fluorescent products when reacted with certain thiols. The reaction is very sensitive, and concentrations as low as 10(-9)M can be detected.
Nakashima
K
K
Faculty of Pharmaceutical Sciences, Nagasaki University, Bunkyo-machi, Nagasaki 852, Japan.
Akimoto
H
H
Nishida
K
K
Nakatsuji
S
S
Akiyama
S
S