Reproduction of postweaning multisystemic wasting syndrome in pigs by prenatal porcine circovirus 2 infection and postnatal porcine parvovirus infection or immunostimulation.
Postweaning multisystemic wasting syndrome (PMWS) was reproduced in prenatally porcine circovirus 2 (PCV2)-infected pigs by either postnatal infection with porcine parvovirus (PPV) or by immunostimulation. Twenty-four randomly selected piglets from 3 sows, which had been experimentally infected during gestation with PCV2, were randomly divided into 3 groups; group 1 (prenatal PCV2 infection, with postnatal PPV infection), group 2 (prenatal PCV2 infection, with postnatal keyhole limpet hemocyanin, emulsified in incomplete Freund's adjuvant [KLH/ICFA] injection), and group 3 (prenatal PCV2 infection only). Twenty-four randomly selected piglets from 3 uninfected sows were randomly divided into 3 groups; group 4 (no prenatal infection, with postnatal PCV2 and PPV infection), group 5 (no prenatal infection, with postnatal PCV2 infection), and group 6 (negative control pigs). Body weight in negative control pigs (group 6) was increased significantly compared with pigs in groups 1, 2, and 4 at 49, 52, 56, 59, and 63 days of age. The granulomatous inflammatory reaction and lymphoid depletion that are typical lesions in pigs with PMWS were observed in the lymph node of piglets in groups 1, 2, and 4 at 63 days of age. Pigs in group 3 had significantly fewer PCV2-positive cells than those from groups 1, 2, 4, or 5. When the prenatally PCV2-infected pigs were infected with PPV or injected with immunostimulant in the postnatal period, they developed PMWS. Thus, factors that potentiate the progression of prenatal PCV2 infection to PMWS are postnatal infection with PPV or immune stimulation.
Ha
Y
Y
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Lee
Y H
YH
Ahn
K-K
KK
Kim
B
B
Chae
C
C
D000375:Aging; D000818:Animals; D001835:Body Weight; D018173:Circoviridae Infections / Q000662:veterinary*; D017925:Circovirus; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D006433:Hemocyanins / Q000276:immunology*; D017403:In Situ Hybridization / Q000662:veterinary; D008198:Lymph Nodes / Q000821:virology; D008297:Male; D010322:Parvoviridae Infections / Q000662:veterinary*; D028323:Parvovirus, Porcine; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000821:virology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D013552:Swine
D004279:DNA, Viral; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin