title: Some new acridinium trifluoromethanesulfonates as spectrophotometric derivatization reagents for aromatic and aliphatic primary amines.
abstract: Some new 9-substituted 10-methylacridinium trifluoromethanesuifonate (triflate) salts have been synthesized and shown to react in methanol with the model aromatic and aliphatic amines, aniline and n-butylamine, to form derivatives which absorb strongly at 445 and 439 nm, respectively. The color development is affected by heat and heating time and by the quantity of acridinium triflate used. A 10-50-fold molar excess of the triflate should be used and the solution heated at 60 degrees for 30 min. The linearity and reproducibility of the assay are improved by the presence of pyridine (for aniline) and triethylamine (for n-butylamine) in the reaction mixture. Beer's law is obeyed over the range 0-1860 ng/ml for aniline and 0-1440 ng/ml for n-butylamine, with each of the new reagents. The relative error and the precision of determination depends on the acridinium triflate used.
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Georgia, Athens, GA 30602, U.S.A.
authors.forename: J W
authors.initials: JW
authors.lastname: Dunning
authors.forename: J T
authors.initials: JT
authors.lastname: Stewart