Effect of bone marrow-derived mesenchymal stem cells on cardiovascular complications in diabetic rats.
BACKGROUND
The purpose of this study was to investigate the effect of mesenchymal stem cells (MSCs) on cardiovascular complications of type 1 diabetes mellitus (DM) in rats.


METHODS
MSCs were derived from the bone marrow of male albino rats. The MSCs were characterized morphologically and by RT-PCR for CD29 expression. They were then infused into female rats which were made diabetic by IP injection of streptozotocin (STZ). The rats were divided into control, STZ, and STZ plus MSC groups. Serum insulin, glucose, and fibrinogen were estimated in all groups and the Y-chromosome gene sry was detected by PCR in pancreatic and cardiac tissues. Physiological cardiovascular functions (heart rate, systolic blood pressure) were assessed by a Langendorff apparatus.


RESULTS
Diabetic rats which received MSCs showed significantly lower serum glucose and increased serum insulin levels compared with the STZ group. Improvement of cardiovascular performance was also observed in the STZ/MSC group compared with the STZ group. The sry gene was detected by PCR in the pancreatic and cardiac tissues of the STZ/MSC group.


CONCLUSIONS
Rat bone marrow harbors cells that have the capacity to differentiate into functional insulin-producing cells capable of controlling blood glucose level in diabetic rats. This may provide a source of cell-based therapy for DM. Furthermore, MSC transplantation can improve cardiac function in DM.<
Abdel Aziz
Mohamed Talaat
MT
Unit of Biochemistry and Molecular Biology, Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt. talaatabdaziz@yahoo.co.uk
El-Asmar
Mohamed Farid
MF
Haidara
Mohamed
M
Atta
Hazem Mahmoud
HM
Roshdy
Nagwa Kamal
NK
Rashed
Laila Ahmed
LA
Sabry
Dina
D
Youssef
Mary Andraws
MA
Abdel Aziz
Ahmed Talaat
AT
Moustafa
Manal
M
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure; D001835:Body Weight; D001854:Bone Marrow Cells / Q000166:cytology*; D002318:Cardiovascular Diseases / Q000209:etiology* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D002454:Cell Differentiation; D002478:Cells, Cultured; D048909:Diabetes Complications / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D007328:Insulin / Q000097:blood; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats
D001786:Blood Glucose; D007328:Insulin; D019012:Integrin beta1