Chromatographic characterization of a molecular imprinted polymer binding cortisol.
A cortisol-binding polymer was prepared by utilising a non-covalent molecular imprinting polymerisation technique. The obtained polymer was packed in a high-performance liquid chromatography (HPLC) column; the selectivity was studied by liquid chromatography, eluting cortisol, cortisone, corticosterone, progesterone, 11-ketoprogesterone, 11alpha-hydroxyprogesterone, 17alpha-hydroxyprogesterone, cortisol 21-hemisuccinate, and cortisol 21-acetate with chloroform, containing 0.5% (v/v) acetic acid, as mobile phase. The mechanism of molecular imprinting was confirmed and a good selectivity for cortisol, with limited recognition for cortisone and 11alpha-hydroxyprogesterone, was found.
Baggiani
C
C
Dipartimento di Chimica Analitica, Università degli Studi di Torino, Via Giuria 5, 10125 Turin, Italy.
Giraudi
G
G
Trotta
F
F
Giovannoli
C
C
Vanni
A
A