Construction of asymmetric cell-sized lipid vesicles from lipid-coated water-in-oil microdroplets.
We present a simple, rapid, and robust method for preparing asymmetric cell-sized lipid bilayer vesicles using water-in-oil (W/O) microdroplets transferred through an oil-water interface. The efficiency for producing cell-sized model membranes is elucidated in relation to the vesicular size and the weight of contained water-soluble molecules. We demonstrate the biological asymmetric nature and the formation of lipid raft microdomain structures using fluorescence microscopy.
Hamada
Tsutomu
T
Miura
Yoko
Y
Komatsu
Yuuki
Y
Kishimoto
Yuko
Y
Vestergaard
Mun'delanji
M
Takagi
Masahiro
M
D008051:Lipid Bilayers; D008055:Lipids / Q000737:chemistry*; D009821:Oils / Q000737:chemistry; D014867:Water / Q000737:chemistry
D008051:Lipid Bilayers; D008055:Lipids; D009821:Oils; D014867:Water