Inheritance and alteration of genome methylation in F1 hybrid rice.
We analyzed the inheritance of DNA methylation in the first filial generation(F1) hybrid of Oryza sativa L. ("Nipponbare"x"Kasalath") by restriction landmark genome scanning (RLGS). Most parental RLGS spots were found in the F1, but eight spots (4%) showed abnormal inheritance: seven of the eight spots were missing in the F1, and one was newly detected in the F1. Here we show demethylation at restriction enzyme sites in the F1. We also found a candidate site of stable heterozygous methylation in the genome. These results show the applicability of the RLGS method for analysis of the inheritance and alteration of methylation in F1 hybrid plants.
Takamiya
Tomoko
T
Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki, Japan.
Hosobuchi
Saeko
S
Noguchi
Tomotsugu
T
Asai
Kenji
K
Nakamura
Eiji
E
Habu
Yoshiki
Y
Paterson
Andrew H
AH
Iijima
Hiroshi
H
Murakami
Yasufumi
Y
Okuizumi
Hisato
H
D002874:Chromosome Mapping; D003198:Computer Simulation; D019175:DNA Methylation; D004262:DNA Restriction Enzymes; D015180:Electrophoresis, Gel, Two-Dimensional; D017343:Genes, Plant; D018745:Genome, Plant; D006824:Hybridization, Genetic; D012275:Oryza / Q000235:genetics*; D018515:Plant Leaves / Q000737:chemistry; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004262:DNA Restriction Enzymes