title: Injection of dsRNA into female A. aegypti mosquitos.
abstract: Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California, Irvine, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Luna
authors.forename: Jennifer
authors.initials: J
authors.lastname: Juhn
authors.forename: Anthony A A
authors.initials: AA
authors.lastname: James
mesh_terms: D000330:Aedes / Q000821:virology; D000818:Animals; D002138:Calibration; D003716:Dengue Virus / Q000302:isolation & purification; D005260:Female; D008845:Microinjections / Q000379:methods*; D009339:Needles; D012330:RNA, Double-Stranded / Q000008:administration & dosage*; D013909:Thorax
chemical_list: D012330:RNA, Double-Stranded