title: Enhanced regeneration of critical bone defects using a biodegradable gelatin sponge and beta-tricalcium phosphate with bone morphogenetic protein-2.
abstract: We examine the osteogenicity of a sponge biomaterial consisting of a biodegradable mixture of gelatin and beta-tricalcium phosphate (betaTCP) that bound bone morphogenetic protein 2 (BMP-2) in critical-sized bone defects in rats. Gelatin-betaTCP sponges containing either phosphate buffered saline or incorporating BMP-2 are implanted into 5 mm diameter bone defects created in rat mandibles. We assess the defects biweekly for 8 weeks following implantation. There is significantly higher osteoinductive activity and significantly more Gla-osteocalcin content at bone-defect healing sites treated with gelatin-betaTCP sponges incorporating BMP-2 than there is in those treated with sponges that did not contain BMP-2. Histologically, new bone that contains bone marrow and that is connected to the original bone almost entirely replaces the regenerated bone. These results show that biodegradable gelatin-betaTCP incorporating BMP-2 is osteogenic enough to promote healing in large bone defects.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kanagawa Dental College 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan. oslab@kdcnet.ac.jp.
authors.forename: Goichi
authors.initials: G
authors.lastname: Matsumoto
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Omi
authors.forename: Eiro
authors.initials: E
authors.lastname: Kubota
authors.forename: Satoru
authors.initials: S
authors.lastname: Ozono
authors.forename: Hideko
authors.initials: H
authors.lastname: Tsuzuki
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Masaya
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D001861:Bone Regeneration; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000627:therapeutic use*; D005780:Gelatin / Q000627:therapeutic use*; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009457:Neuroglia / Q000378:metabolism; D015675:Osteocalcin / Q000737:chemistry; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D013552:Swine
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D015675:Osteocalcin; D005780:Gelatin