title: Suppression of anaphylactic reaction in murine bySiegesbeckia pubescens.
abstract: The aqueous extract ofSiegesbeckia pubescens (SPAE) inhibited compound 48/80-induced systemic anaphylaxis 100% with the dose of 1.0, 0.5 mg/g body weight (BW) at 1 h before or 5 min, 10 min after intraperitoneal injection of compound 48/80. The passive cutaneous anaphylaxis (PCA) reaction also inhibited to 78.5% by oral administration of SPAE (1.0 mg/g BW). When SPAE pretreated on mice at concentrations ranging from 0.0001 to 1.0 mg/g BW, the serum histamine levels were reduced in a dose-dependent manner. Moreover, SPAE (100-800 mug/ml) dose-dependently inhibited the histamine release from peritoneal mast cells (RPMC) by compound 48/80 (5 mug/ml). Analysis by microscopic appearance observation revealed that SPAE (500 mug/ml) stabilized the RPMC membrane. Therefore, these findings indicate that SPAE inhibits anaphylactic reactions through stabilization of mast cell membrane.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, 570-749, Chonbuk, Korea.
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: C Y
authors.initials: CY
authors.lastname: Kim
authors.forename: M H
authors.initials: MH
authors.lastname: Kwon
authors.forename: T Y
authors.initials: TY
authors.lastname: Shin
authors.forename: E J
authors.initials: EJ
authors.lastname: Lee