Application of solid waste from anaerobic digestion of poultry litter in Agrocybe aegerita cultivation: mushroom production, lignocellulolytic enzymes activity and substrate utilization.
The degradation and utilization of solid waste (SW) from anaerobic digestion of poultry litter by Agrocybe aegerita was evaluated through mushroom production, loss of organic matter (LOM), lignocellulolytic enzymes activity, lignocellulose degradation and mushroom nutrients content. Among the substrate combinations (SCs) tested, substrates composed of 10-20% SW, 70-80% wheat straw and 10% millet was found to produce the highest mushroom yield (770.5 and 642.9 g per 1.5 kg of substrate). LOM in all SCs tested varied between 8.8 and 48.2%. A. aegerita appears to degrade macromolecule components (0.6-21.8% lignin, 33.1-55.2% cellulose and 14-53.9% hemicellulose) during cultivation on the different SCs. Among the seven extracellular enzymes monitored, laccase, peroxidase and CMCase activities were higher before fruiting; while xylanase showed higher activities after fruiting. A source of carbohydrates (e.g., millet) in the substrate is needed in order to obtain yield and biological efficiency comparable to other commercially cultivated exotic mushrooms.
Isikhuemhen
Omoanghe S
OS
Mushroom Biology & Fungal Biotechnology Laboratory, Department of Natural Resources and Environmental Design, North Carolina A&T State University, Greensboro, NC 27407, USA. omon@ncat.edu
Mikiashvili
Nona A
NA
Kelkar
Vinaya
V
D055362:Agrocybe / Q000201:enzymology* / Q000254:growth & development; D000693:Anaerobiosis; D000818:Animals; D001673:Biodegradation, Environmental; D002480:Cellulase / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D048690:Fruiting Bodies, Fungal / Q000737:chemistry / Q000254:growth & development; D044643:Glucan 1,4-beta-Glucosidase / Q000378:metabolism; D042845:Laccase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D008372:Manure; D009195:Peroxidase / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D011200:Poultry; D012037:Refuse Disposal / Q000379:methods*; D014995:Xylosidases / Q000378:metabolism
D008372:Manure; D011134:Polysaccharides; C036909:lignocellulose; C007916:hemicellulose; D008031:Lignin; D042845:Laccase; D009195:Peroxidase; D014995:Xylosidases; D002480:Cellulase; C019826:carboxymethylcellulase; D044643:Glucan 1,4-beta-Glucosidase; D043364:Endo-1,4-beta Xylanases; D043366:Cellulose 1,4-beta-Cellobiosidase