Determination of clenbuterol in pharmaceutical preparations by reaction with o-phthalaldehyde using a flow-injection fluorimetric procedure.
A new procedure for the determination of clenbuterol is proposed using flow-injection and fluorimetric detection. The method is based on the derivatization reaction of the primary amine group with o-phthalaldehyde in the presence of 2-mercaptoethanol. The calibration graph based on peak area was linear in the range 0.2-5 mug ml(-1) and the detection limit was 0.06 mug ml(-1). The method was validated using a reference spectrophotometric procedure and was applied to the determination of the drug in commercial pharmaceutical preparations.
López-Erroz
C
C
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, E-30071 Murcia, Spain.
Viñas
P
P
Cerdán
F J
FJ
Hernández-Córdoba
M
M