[Comparison of two rat models of IgA nephropathy].
OBJECTIVE
To study the methods for rapid establishment of rat models of IgA nephropathy.


METHODS
Forty female SD rats weighing 160-200 g were randomized into 3 groups. In group A, the rats received intravenous injection of staphylococcal enterotoxin B (SEB) and oral bovine serum albumin (BSA), and in group B, CCl4 was injected subcutaneously in addition to the above treatments; the rats in group C received no treatments to serve as the normal control group. The rats were sacrificed 10 and 14 weeks after the treatment for biochemical testing of the arterial blood and histopathological and IgA immunofluorescence examination of the renal tissues. The twenty-four-hour urine was collected at 10, 12, and 14 weeks after the treatments for detecting the urine proteins.


RESULTS
Compared with the control group, the rats in groups A and B showed significantly increased serum creatinine, urine nitrogen and protein levels. Pathological examination of the renal tissue showed mild to moderate mesangial expansion and mesangial cell proliferation in groups A and B, without obvious difference between the two groups; but hematuria and proteinuria occurred earlier in group B with stronger IgA immunofluorescence than in group A.


CONCLUSIONS
Both of the methods used in group A and group B can successfully induce IgA nephropathy in rats, but in group B, hematuria and urineprotein occurs earlier and IgA immunofluorescence is more stronger. Therefore intravenous SEB injection combined with oral BSA and subcutaneous CCl4 administration is a better method for time-efficient establishment of rat models of IgA nephropathy.
Peng
Wei
W
Department of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. phhw117 @hotmail.com
Liu
Zheng-rong
ZR
D000818:Animals; D001427:Bacterial Toxins; D002251:Carbon Tetrachloride; D004195:Disease Models, Animal; D005260:Female; D005922:Glomerulonephritis, IGA / Q000139:chemically induced* / Q000473:pathology; D006460:Hemolysin Proteins; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine; D013108:Sphingomyelin Phosphodiesterase
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D012710:Serum Albumin, Bovine; D002251:Carbon Tetrachloride; D013108:Sphingomyelin Phosphodiesterase; C029878:hlb protein, Staphylococcus aureus