Spectrophotometric determination of tetracyclines in pharmaceutical preparations, with uranyl acetate.
Uranyl acetate is proposed as a reagent for the spectrophotometric determination of the tetracycline group of antibiotics. The reagent forms orange-red 1:1 complexes with the drugs in N,N-dimethylformamide medium. The complexes show absorption maxima at 414, 406, 419, 405 and 402 nm for tetracycline hydrochloride (TCH), oxytetracycline hydrochloride (OTCH), chlortetracycline hydrochloride (CTCH), doxycycline hydrochloride (DCH) and methacycline hydrochloride (MCH), respectively. Beer's law is valid over the concentration ranges 0-115, 0-120, 0-125, 0-135 and 0-110 microg/ml for TCH, OTCH, CTCH, DCH and MCH, respectively.
Saha
U
U
Central Public Health & Drug Laboratory, 2 Convent Lane, Calcutta 700016, India.
Sen
A K
AK
Das
T K
TK
Bhowal
S K
SK