Establishment of cell lines from mouse embryos with early embryonic lethality.
It is often difficult to determine molecular mechanisms leading to early embryonic lethality of genetically modified mice due to lack of cells for further analyses. The authors describe here establishment of mouse embryonic fibroblast (MEF) cell lines from gastrulation stage embryos. In this example, using a combination of in vivo and in vitro techniques, the authors successfully generated MEF cell lines that lack both fibronectin (FN) and focal adhesion kinase (FAK).
Howerton
Kyle
K
Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA.
Schlaepfer
David D
DD
Ilic
Dusko
D
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology*; D005353:Fibronectins / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D055786:Gene Knockout Techniques; D005804:Genes, Lethal; D051379:Mice
D005353:Fibronectins; D051416:Focal Adhesion Protein-Tyrosine Kinases