Injection of An. stephensi embryos to generate malaria-resistant mosquitoes.
The introduction of exogenous genes into the genomes of mosquitoes requires microinjection techniques tailored to the specific species of interest. This video protocol demonstrates a method used by the James laboratory to microinject DNA constructs into Anopheles stephensi embryos for the generation of transformed mosquitoes. Techniques for preparing microinjection needles, collecting and preparing embryos and performing the microinjection are illustrated.
Terenius
Olle
O
Department of Molecular Biology and Biochemistry, University of California, Irvine, USA. olle.terenius@uci.edu
Juhn
Jennifer
J
James
Anthony A
AA
D000818:Animals; D000852:Anopheles / Q000196:embryology* / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage; D018014:Gene Transfer Techniques; D007113:Immunity, Innate / Q000235:genetics*; D008288:Malaria / Q000517:prevention & control*; D008845:Microinjections / Q000379:methods
D004247:DNA