Preparation and evaluation of non-bonded hyperbranched polymer-coated columns for capillary electrophoresis.
A series of hyperbranched poly(amine-ester)s based on 1,1,1-trimethylolpropane, methyl acrylate and diethanolamine were synthesized and coated on the inner surface of the fused-silica capillaries by physical adsorption. The most effective coating was the seventh generation hyperbranched poly(amine-ester) coating, which reduced the electroosmotic flow (EOF) greatly and suppressed protein adsorption effectively. The high separation efficiencies for basic proteins were obtained and the coating had a good stability.
Shou
Chong-Qi
CQ
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; Department of Applied Chemistry, Jinan University, Jinan 250022, PR China.
Zhou
Chang-Li
CL
Zhao
Chun-Bin
CB
Zhang
Zhi-Liang
ZL
Li
Guan-Bin
GB
Chen
Li-Ren
LR