The microscopic biological response of human chondrocytes to bovine bone scaffold.
This study was performed to determine the microscopic biological response of human nasal septum chondrocytes and human knee articular chondrocytes placed on a demineralized bovine bone scaffold. Both chondrocytes were cultured and seeded onto the bovine bone scaffold with seeding density of 1 x 105 cells per 100 microl/scaffold and incubated for 1, 2, 5 and 7 days. Proliferation and viability of the cells were measured by mitochondrial dehydrogenase activity (MTT assay), adhesion study was analyzed by scanning electron microscopy and differentiation study was analyzed by immunofluorescence staining and confocal laser scanning electron microscopy. The results showed good proliferation and viability of both chondrocytes on the scaffolds from day 1 to day 7. Both chondrocytes increased in number with time and readily grew on the surface and into the open pores of the scaffold. Immunofluorescence staining demonstrated collagen type II on the scaffolds for both chondrocytes. The results showed good cells proliferation, attachment and maturity of the chondrocytes on the demineralized bovine bone scaffold. The bovine bone being easily resourced, relatively inexpensive and non toxic has good potential for use as a three dimensional construct in cartilage tissue engineering.
Abdullah
Baharudin
B
Universiti Sains Malaysia, Kubang Kerian, Malaysia. baharudin@kb.usm.my
Shibghatullah
Abdul Halim
AH
Hamid
Suzina Sheikh Abdul
SS
Omar
Nor Shamsuria
NS
Samsuddin
Abd Rani
AR
D000818:Animals; D018775:Bone Demineralization Technique; D018786:Bone Substitutes; D002356:Cartilage / Q000166:cytology / Q000502:physiology*; D002417:Cattle; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology* / Q000648:ultrastructure; D024043:Collagen Type II / Q000096:biosynthesis; D006801:Humans; D010012:Osteogenesis / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D018786:Bone Substitutes; D024043:Collagen Type II