Protective effect of anthocyanins from black soybean seed coats on UVB-induced apoptotic cell death in vitro and in vivo.
UVB radiation proves to be one of the most relevant environmental risks because of its hazardous effects, such as premature skin aging and especially skin photocarcinogenesis. Anthocyanins, water-soluble pigments present in plants, are known to be powerful antioxidants that help protect plants from UV damage. In this study, we aimed at investigating the protective effect of anthocyanins from black soybean [Glycine max (L.) Merr] seed coats on UVB-induced apoptosis, and furthermore, we investigated the molecular mechanism responsible for regulation of apoptosis in vitro and in vivo. Pretreatment with anthocyanins reduced UVB-induced reactive oxygen species levels and inhibited UVB-induced apoptotic cell death through the prevention of caspase-3 pathway activation and reduction of proapoptotic Bax protein levels. UVB irradiation induced apoptotic cell death, which was inhibited by topical application of anthocyanins in hairless mice. It is concluded that anthocyanins from the seed coat of black soybeans may be useful compounds to modulate UVB-induced photoaging.
Tsoyi
Konstantin
K
Department of Pharmacology, School of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.
Park
Hyung Bin
HB
Kim
Young Min
YM
Chung
Jong Il
JI
Shin
Sung Chul
SC
Shim
Hae Jung
HJ
Lee
Won Suk
WS
Seo
Han Geuk
HG
Lee
Jae Heun
JH
Chang
Ki Churl
KC
Kim
Hye Jung
HJ
D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D061945:Caspase Inhibitors; D002461:Cell Line, Transformed; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D015603:Keratinocytes; D051379:Mice; D008812:Mice, Hairless; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D012639:Seeds / Q000737:chemistry*; D012867:Skin / Q000166:cytology; D013025:Glycine max / Q000737:chemistry*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000872:Anthocyanins; D061945:Caspase Inhibitors; D004791:Enzyme Inhibitors; D017382:Reactive Oxygen Species