title: Production, isolation and biological activity of nargenicin from Nocardia sp. CS682.
abstract: Culture broth of an actinomycete isolate, Nocardia sp. CS682 showed specifically higher antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA). Purified substance from the organism, CS-682, which is active against MRSA and Micrococcus leuteus, is a C(28)H(37)NO(8) (M+H(+), observed: 516.83) and identified as an unusual macrolide antibiotic, nargenicin. The chemical structure of CS-682 was identified by FT-IR, (1)H-NMR, (13)C-NMR, and ((1)H-(1)H and (1)H-(13)H) COSY. The anti-MRSA activity of CS-682 was stronger than that of oxacillin, vancomycin, monensin, erythromycin, and spiramycin. Phylogenetic analysis showed that strain CS682 is closely related to Nocardia tenerifensis DSM 44704(T) (98.7% sequence similarity), followed by N. brasiliensis ATCC 19296(T) (98.4% sequence similarity). The ability of Nocardia sp. CS682 to produce nargenicin was unique.
authors.affiliation: Department of Pharmaceutical Engineering, Sun-Moon University, Asan, Chungnam 337-708, Korea.
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
authors.forename: Tokutaro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Chi Nam
authors.initials: CN
authors.lastname: Seong
authors.forename: Keun Sik
authors.initials: KS
authors.lastname: Baik
authors.forename: Seong Chan
authors.initials: SC
authors.lastname: Park
authors.forename: Hyo Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: So Young
authors.initials: SY
authors.lastname: Jang
authors.forename: Jaya Ram
authors.initials: JR
authors.lastname: Simkhada
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Yoo
mesh_terms: D039903:Actinobacteria / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000900:Anti-Bacterial Agents; D001419:Bacteria / Q000187:drug effects; D002627:Chemistry, Physical; D002876:Chromosomes, Bacterial / Q000737:chemistry; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D024881:Drug Resistance, Bacterial; D007783:Lactones / Q000737:chemistry / Q000494:pharmacology; D016106:Methicillin Resistance / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009615:Nocardia / Q000737:chemistry* / Q000145:classification; D012988:Soil Microbiology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D007783:Lactones; C034624:nargenicin A1