The use of fluorescent probe molecules for detection in capillary electrokinetic separations.
On-column fluorescence labeling using hydrophobic probe molecules is described for the enhancement of detection in capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MECC). Experimental details are presented for the detection of alkyl sulfate surfactant/micelle systems using the probe 2-p-toluidinonaphthalene-6-sulfonate. Using this labeling scheme, laser fluorimetry is employed to determine sodium dodecyl sulfate in a generic, commercial soap product. The influence of probe molecule concentration on calibration plots and analyte mobility is also presented. The utility of indirect detection for MECC is demonstrated with the addition of 1-anilinonaphthalene-8-sulfonate to the micellar mobile phase. A brief discussion of the analytical merits of this mode of detection is presented.
Swaile
D F
DF
Department of Chemistry, The University of Tennessee, Knoxville, TN 37996-1600, USA.
Copper
C L
CL
Sepaniak
M J
MJ
Burton
D E
DE
Powell
L L
LL