[Role of alpha-tocopherol in oxidative stress of rat thymocytes induced by hydrogen peroxide and menadione].
T paper deals with studying the effect of alpha-tocopherol and its analogues (alpha-tocopheryl acetate and alpha-tocopheryl quinine) showing no antioxidant properties on rat thymocytes survival and intracellular content of reactive oxygen species (ROS) at H2O2 and menadione-induced oxidative stress. It is established, that the ability of alpha-tocopherol to inhibit the thymocytes destruction induced by the development of oxidative stress is insignificant. It does not depend on the presence of free OH-group in the structure of alpha-tocopherol molecule responsible for development of antioxidant properties, and does not correlate with its influence on intracellular ROS content. The efficiency of the glutathione synthesis predecessor N-acetyl-L-cysteine in the given model testifies to the involving of other systems of antioxidative protection in these processes. The obtained data allow to conclude, that alpha-tocopherol does not play the main role in the protection of cells against ROS damaging action that calls into question its ability to prevent the oxidative stress.
Petrova
G V
GV
Donchenko
G V
GV
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D024505:Tocopherols / Q000494:pharmacology; D014810:Vitamin E / Q000031:analogs & derivatives / Q000494:pharmacology; D024483:Vitamin K 3 / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D014810:Vitamin E; D024483:Vitamin K 3; C002421:tocopherylquinone; D006861:Hydrogen Peroxide; D024502:alpha-Tocopherol; D024505:Tocopherols