title: Quantitative analysis of protein-RNA interactions by gel mobility shift.
abstract: The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to resolve free from bound RNA with high resolution in a gel matrix. We review the quantitative application of this approach to elucidate thermodynamic properties of protein-RNA complexes. Assay designs for titration, competition, and stoichiometry experiments are presented for two unrelated model complexes.
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Worcester, Massachusetts, USA.
authors.forename: Sean P
authors.initials: SP
authors.lastname: Ryder
authors.forename: Michael I
authors.initials: MI
authors.lastname: Recht
authors.forename: James R
authors.initials: JR
authors.lastname: Williamson
mesh_terms: D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011506:Proteins; D012313:RNA