Angiopoietin-1, but not platelet-derived growth factor-AB, is a cooperative stimulator of vascular endothelial growth factor A-accelerated endothelial cell scratch closure.
Wound healing and the grow-in of free tissue grafts critically depend on blood vessel growth, i.e., on the angiogenic invasion of endothelial cells, which is critically reduced in smokers, in patients suffering from microangiopathies (e.g., in diabetes), or in those who are treated with immunosuppressives. Although several angiogenic factors have been tested to accelerate wound healing in such critically patients, their combinations have not yet been systematically investigated. This study was done to reveal which combination of proangiogenic with promaturating factors is the most effective in an endothelial wound closure assay. Human umbilical vein endothelial cells were isolated, cultured to confluence, and subjected to a scratch wound assay with the addition of vascular endothelial growth factor (VEGF)-A(165), platelet-derived growth factor (PDGF)-AB, angiopoietin-1 (ANG1), or ANG2 and all of their 16 possible combinations. VEGF-A(165) plus ANG1 was most effective at accelerating endothelial scratch closure. Moreover, VEGF-A(165) stimulated wound closure in all combinations tested, while it was attenuated by PDGF-AB. Thus, with respect to their effects on endothelial cells, a combination of VEGF-A with ANG1 is the most promising and is superior to combinations with PDGF-AB.
Alter
Alexander
A
Department of Prosthetic Dentistry, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany.
Schmiedeck
Dorothee
D
Fussnegger
Markus R
MR
Pries
Axel R
AR
Freesmeyer
Wolfgang B
WB
Zakrzewicz
Andreas
A
D042683:Angiopoietin-1 / Q000378:metabolism*; D042702:Angiopoietin-2 / Q000378:metabolism; D002465:Cell Movement; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014945:Wound Healing
D042683:Angiopoietin-1; D042702:Angiopoietin-2; D010982:Platelet-Derived Growth Factor; D042461:Vascular Endothelial Growth Factor A; C095477:platelet-derived growth factor AB