title: Fluorimetric determination of ascorbic acid with o-phenylenediamine.
abstract: A simple and sensitive fluorimetric method for the determination of ascorbic acid (AA) is described. The method is based on the condensation reaction between AA and o-phenylenediamine (OPDA) in the absence of the oxidant. The fluorescence intensity is measured at excitation and emission wavelengths of 360 and 430 nm, respectively. Under optimum condition, a linear relationship is obtained between the fluorescence intensity and the concentration of AA in the range of 0.05-40 mug ml(-1). The detection limit is 0.006 mug ml(-1), which is obviously lower than that of other fluorimetric methods reported.
authors.affiliation: Key Laboratory for Colloid and Interface Chemistry of Education Ministry, Department of Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Diao
authors.forename: Changxia
authors.initials: C
authors.lastname: Sun
authors.forename: Jinghe
authors.initials: J
authors.lastname: Yang
authors.forename: Yuebo
authors.initials: Y
authors.lastname: Wang
authors.forename: Shuna
authors.initials: S
authors.lastname: Sun