title: Mechanical properties of radiation-sterilised human Bone-Tendon-Bone grafts preserved by different methods.
abstract: Patellar tendon auto- and allo-grafts are commonly used in orthopedic surgery for reconstruction of the anterior cruciate ligaments (ACL). Autografts are mainly used for primary reconstruction, while allografts are useful for revision surgery. To avoid the risk of infectious disease transmission allografts should be radiation-sterilised. As radiation-sterilisation supposedly decreases the mechanical strength of tendon it is important to establish methods of allograft preservation and sterilisation assuring the best quality of grafts and their safety at the same time. Therefore, the purpose of this study was to compare the tensile strength of human patellar tendon (cut out as for ACL reconstruction), preserved by various methods (deep fresh freezing, glycerolisation, lyophilisation) and subsequently radiation-sterilised with doses of 0, 25, 50 or 100 kGy. Bone-Tendon-Bone grafts (BTB) were prepared from cadaveric human patella tendons with both patellar and tibial attachments. BTB grafts were preserved by deep freezing, glycerolisation or lyophilisation and were subsequently radiation-sterilised with doses of 0 (control), 25, 50 or 100 kGy. All samples were subjected to mechanical failure tensile tests with the use of Instron system in order to estimate their mechanical properties. All lyophilised grafts were rehydrated before performing of those tests. Obtained mechanical tests results of examined grafts suggest that deep-frozen irradiated grafts retain their initial mechanical properties to an extent which does not exclude their clinical application.
authors.affiliation: Department of Transplantology and Central Tissue Bank, Medical University of Warsaw, Chałubińskiego 5, 02-004, Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Kamiński
authors.forename: G
authors.initials: G
authors.lastname: Gut
authors.forename: J
authors.initials: J
authors.lastname: Marowska
authors.forename: M
authors.initials: M
authors.lastname: Lada-Kozłowska
authors.forename: W
authors.initials: W
authors.lastname: Biwejnis
authors.forename: M
authors.initials: M
authors.lastname: Zasacka
mesh_terms: D000328:Adult; D016118:Anterior Cruciate Ligament / Q000601:surgery; D050376:Bone-Patellar Tendon-Bone Grafting / Q000502:physiology*; D002102:Cadaver; D005612:Freeze Drying; D005615:Freezing; D005990:Glycerol; D006801:Humans; D008875:Middle Aged; D017847:Patellar Ligament / Q000502:physiology / Q000528:radiation effects* / Q000637:transplantation; D011829:Radiation Dosage; D013242:Sterilization / Q000379:methods*; D013718:Tensile Strength / Q000502:physiology / Q000528:radiation effects*; D013977:Tibia / Q000502:physiology / Q000528:radiation effects* / Q000637:transplantation; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D005990:Glycerol