title: Comparison between the performances of amperometric immunosensors for cholera antitoxin based on three enzyme markers.
abstract: We developed a novel copolymer modified amperometric immunosensor for the detection of cholera antitoxin (anti-CT), by the electropolymerization of pyrrole-biotin and pyrrole-lactitobionamide monomers on platinum or glassy carbon electrodes. In the detection of cholera antitoxin we have used three enzymatic marker detection systems based on HRP-labeled rabbit IgG antibodies, biotinylated polyphenol-oxidase (PPO-B) and biotinylated glucose-oxidase (GOX-B). The comparison of the electro-enzymatic performances of these three configurations with different substrates, clearly shows that the more sensitive amperometric immunosensor was based on HRP with a lower limit of detection of 50ng/ml anti-CT using hydroquinone/H(2)O(2) system. The response time for this substrate was in range of 5-30s. The HRP-amperometric immunosensor has thus proven to be a very sensitive tool to monitor nanomolar concentrations of anti-CT.
authors.affiliation: Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
authors.forename: Rodica E
authors.initials: RE
authors.lastname: Ionescu
authors.forename: Chantal
authors.initials: C
authors.lastname: Gondran
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.forename: Levi A
authors.initials: LA
authors.lastname: Gheber
authors.forename: Robert S
authors.initials: RS
authors.lastname: Marks