title: Relaxed specificity of the Bacillus subtilis TatAdCd translocase in Tat-dependent protein secretion.
abstract: Protein translocation via the twin arginine translocation (TAT) pathway is characterized by the translocation of prefolded proteins across the hydrophobic lipid bilayer of the membrane. In Bacillus subtilis, two different Tat translocases are involved in this process, and both display different substrate specificities: PhoD is secreted via TatAdCd, whereas YwbN is secreted via TatAyCy. It was previously assumed that both TatAy and TatCy are essential for the translocation of the YwbN precursor. Through complementation studies, we now show that TatAy can be functionally replaced by TatAd when the latter is offered to the cells in excess amounts. Moreover, under conditions of overproduction, TatAdCd, in contrast to TatAyCy, shows an increased tolerance toward the acceptance of various Tat-dependent proteins.
authors.affiliation: Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
authors.forename: Robyn T
authors.initials: RT
authors.lastname: Eijlander
authors.forename: Jan D H
authors.initials: JD
authors.lastname: Jongbloed
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
mesh_terms: D001120:Arginine / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers; D005784:Gene Amplification; D055786:Gene Knockout Techniques; D015696:Gene Products, tat / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D010458:Peptidyl Transferases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D021381:Protein Transport / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D015696:Gene Products, tat; D026901:Membrane Transport Proteins; D001120:Arginine; D010458:Peptidyl Transferases