title: Staphylococcus aureus surface protein SasG contributes to intercellular autoaggregation of Staphylococcus aureus.
abstract: Staphylococcus aureus surface protein G (SasG) is one of cell surface proteins with cell-wall sorting motif. The sasG mutant showed significantly reduced cell aggregation and biofilm formation. SasG is comprised of variable A domain and multiple tandem repeats of B domain, native-PAGE and in vitro formaldehyde cross-linking experiments revealed that the recombinant protein of the A domain showed homo-oligomerization as an octamer, but B domain did not. This study shows that SasG-A domain contributes to intercellular autoaggregation by homo-oligomerization, and that may facilitate the adherence to host-tissues in the infection of S. aureus.
authors.affiliation: Laboratory of Bacterial Genomics, Center for Pathogen Genomics, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. makokuro@nih.go.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Kuroda
authors.forename: Ryuta
authors.initials: R
authors.lastname: Ito
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.forename: Kimio
authors.initials: K
authors.lastname: Matoba
authors.forename: Shinji
authors.initials: S
authors.lastname: Saito
authors.forename: Isao
authors.initials: I
authors.lastname: Tanaka
authors.forename: Toshiko
authors.initials: T
authors.lastname: Ohta
mesh_terms: D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D002462:Cell Membrane; D004252:DNA Mutational Analysis; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D017434:Protein Structure, Tertiary / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C524374:SasG protein, Staphylococcus aureus