Diagnosis of human hepatitis B virus in non-treated blood by the bovine IgG DNA-linked carbon nanotube biosensor.
OBJECTIVE
To identify an effective bioassay for human hepatitis B virus (HBV) using voltammetric sensor.


METHODS
Electrode was prepared with bovine IgG immobilized onto a DNA-linked carbon nanotube electrode (BIDCE). Analytical BIDCE conditions were optimized through square wave (SW) stripping and cyclic voltammetry (CV).


RESULTS
Optimum parameters of 75 Hz SW frequency, 0.1 V SW amplitude, -1.3 V SW accumulation potential, 100 s accumulation time, and 3.0 mV SW increment potential were obtained. Within working ranges of 0.035-0.242 mg/mL anti-bovine IgG, the relative standard deviation of 0.2 mL HBV was observed to be 0.04 (n=4).


CONCLUSIONS
Diagnostic application was performed through direct assay of HBV in non-treated human blood.
Ly
Suw Young
SY
Biosensor Research Institute, Seoul National University of Technology, 172, Gongreung 2-dong, Nowon-gu, Seoul 139-743, South Korea. suwyoung@snut.ac.kr
Cho
Nam Sun
NS
D000818:Animals; D000914:Antibodies, Viral; D015374:Biosensing Techniques / Q000379:methods*; D001769:Blood / Q000821:virology*; D002417:Cattle; D006509:Hepatitis B / Q000175:diagnosis*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G; D037742:Nanotubes, Carbon
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D037742:Nanotubes, Carbon