Reversed-phase liquid chromatographic retention behaviour of catechol amino-acids.
For a group of catechol amino-acids varying widely in acid strength and hydrophobicity, the effects of mobile phase composition, pH and ionic strength on their reversed-phase chromatographic separation have been determined, with phosphate buffer as mobile phase. Retention data were measured for 18 catecholamine derivatives. The retardation factors and retention behaviour of all the compounds tested could be explained in terms of the acid dissociation and tautomeric constants.
Ishimitsu
T
T
Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607, Japan.
Hirose
S
S
Sakuri
H
H