Specific interaction of TFII-I with an upstream element on the HIV-1 LTR regulates induction of latent provirus.
RBF-2 is a factor comprised of a USF1/2 heterodimer, whose association with a highly conserved upstream element (RBEIII) on the HIV-1 LTR requires a co-factor TFII-I. We have identified specific nucleotides, immediately 3' of RBEIII that are required for stable association of TFII-I with this region of the LTR. Mutations that inhibit interaction of TFII-I with DNA also prevent stimulation of USF binding to RBEIII, and render the integrated LTR unresponsive to T cell signaling. These results demonstrate an essential role of TFII-I bound at an upstream LTR element for viral replication.
Malcolm
Tom
T
Department of Biochemistry and Molecular Biology, Molecular Epigenetics, LSI, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3.
Kam
Joanna
J
Pour
Pouya Sadeghi
PS
Sadowski
Ivan
I
D001483:Base Sequence; D015967:Gene Expression Regulation, Viral; D016325:HIV Long Terminal Repeat / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D009154:Mutation; D011533:Proviruses / Q000235:genetics / Q000502:physiology*; D019473:Transcription Factors, TFII / Q000378:metabolism*; D051720:Upstream Stimulatory Factors / Q000378:metabolism; D014775:Virus Activation / Q000235:genetics*
C104259:GTF2I protein, human; D019473:Transcription Factors, TFII; D051720:Upstream Stimulatory Factors