Establishment of CD5 and CD10 double-positive mature B-cell line, WILL1, showing complex 8q24 translocation involving 14q32 and 6q27.
We established a novel mature B-cell line from a CD5 and CD10 double-positive diffuse large B-cell lymphoma patient, designated as WILL1. WILL1 cells were positive for CD5, CD10, CD19, and CD20. Spectral karyotype (SKY) analysis revealed chromosome 8 signals on 6q27 as well as 14q32. Fluorescence in situ hybridization (FISH) analysis suggested that a translocation break occurred outside the immunoglobulin heavy chain (IGH) gene on 14q32. Moreover, fusion signals of IGH and C-MYC probes were detected on the derivative 6 and derivative 14 chromosomes. Southern blot analysis using a C-MYC exon II fragment failed to detect rearrangement, suggesting that the 8q24 breakpoints lay far up- or downstream of the C-MYC gene. WILL1 is a useful tool to analyze the pathogenesis of CD5 and CD10 double-positive diffuse large B-cell lymphoma, and for molecular cloning of the unique translocation breakpoints of 14q32 and 8q24 and a novel gene on 6q27.
Uneda
Shima
S
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan.
Gotoh
Minako
M
Department of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Sonoki
Takashi
T
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan. sonoki@wakayama-med.ac.jp.
Nishida
Kazuhiro
K
Department of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Nakamura
Yasushi
Y
Department of Clinical Laboratory Medicine, Wakayama Medical University, Wakayama, Japan.
Kurimoto
Miwa
M
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan.
Hanaoka
Nobuyoshi
N
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan.
Matsuoka
Hiroshi
H
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan.
Taniwaki
Masafumi
M
Department of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Nakakuma
Hideki
H
Department of Hematology/Oncology, Wakayama Medical University, Kimi-idera 811-1, Wakayama, 641-8510, Japan.
D000369:Aged, 80 and over; D044466:Asian People; D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology*; D018956:CD5 Antigens; D045744:Cell Line, Tumor / Q000378:metabolism* / Q000473:pathology*; D002877:Chromosomes, Human / Q000378:metabolism; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000378:metabolism; D007564:Japan; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D015260:Neprilysin; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D014178:Translocation, Genetic
D018956:CD5 Antigens; D007143:Immunoglobulin Heavy Chains; C489427:MYC protein, human; D015514:Oncogene Proteins, Fusion; D016271:Proto-Oncogene Proteins c-myc; D015260:Neprilysin