title: Combination of intraoperative 5-aminolevulinic acid-induced fluorescence and 3-D MR imaging for guidance of robotic laser ablation for precision neurosurgery.
abstract: A combination of 5-aminolevulinic acid (5-ALA)-induced fluorescence and three-dimensional (3-D) magnetic resonance imaging (MRI) of a brain tumor has been incorporated into a robotic laser ablation neurosurgery system. 5-ALA is a non-fluorescent prodrug that leads to intracellular accumulation of fluorescent protoporphyrins IX (PpIX) in malignant glioma. The PpIX tends to accumulate in pathological lesions, and emits red fluorescence when excited by blue light. This fluorescence is illuminated with laser excitation, enables intra-operative identification of the position of a tumor and provides guidance for resection with laser photocoagulation. The information provided by the MRI is enhanced by the 5-ALA fluorescence data, and this enhanced information is integrated into a robotic laser ablation system. The accuracy of the fluorescent measurement of the tumor is improved using high-precision spectral analysis. The fluorescence assists in the detection of malignant brain tumors intraoperatively and improves their removal rate.
authors.affiliation: Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. liao@bmpe.t.u-tokyo.ac.jp
authors.forename: Hongen
authors.initials: H
authors.lastname: Liao
authors.forename: Koji
authors.initials: K
authors.lastname: Shimaya
authors.forename: Kaimeng
authors.initials: K
authors.lastname: Wang
authors.forename: Takashi
authors.initials: T
authors.lastname: Maruyama
authors.forename: Masafumi
authors.initials: M
authors.lastname: Noguchi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Muragaki
authors.forename: Etsuko
authors.initials: E
authors.lastname: Kobayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iseki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sakuma
mesh_terms: D000622:Aminolevulinic Acid; D001932:Brain Neoplasms / Q000175:diagnosis / Q000601:surgery*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes; D006801:Humans; D053685:Laser Therapy / Q000295:instrumentation*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D019635:Neurosurgical Procedures / Q000295:instrumentation*; D012371:Robotics / Q000295:instrumentation*; D013382:Subtraction Technique / Q000295:instrumentation; D025321:Surgery, Computer-Assisted / Q000295:instrumentation*; D018511:Systems Integration
chemical_list: D005456:Fluorescent Dyes; D000622:Aminolevulinic Acid