Binding enhancement of antigen-functionalized PEGylated gold nanoparticles onto antibody-immobilized surface by increasing the functionalized antigen using alpha-sulfanyl-omega-amino-PEG.
We established a technique for constructing PEGylated gold nanoparticles (GNP) that have small compounds with almost complete functionalities on their surfaces using a newly synthesized hetero-telechelic poly(ethylene glycol) (PEG), and their association/dissociation behavior on an antibody-immobilized surface was evaluated using a surface plasmon resonance (SPR) sensor.
Yoshimoto
Keitaro
K
Graduate School of Pure and Applied Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki, Japan.
Hoshino
Yuki
Y
Ishii
Takehiko
T
Nagasaki
Yukio
Y
D055910:Antibodies, Immobilized / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000737:chemistry*; D001665:Binding Sites; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013997:Time Factors
D055910:Antibodies, Immobilized; D000941:Antigens; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; D006046:Gold