title: Characterization of exopolysaccharide (EPS) produced by Weissella hellenica SKkimchi3 isolated from kimchi.
abstract: Weissella hellenica SKkimchi3 produces the higher exopolysaccharide (EPS) on sucrose than lactose, glucose, and fructose at pH 5 and 20 degrees C. Sucrose was exclusively used to cultivate SKkimchi3 in all experiments base on the EPS production tests. The molecular mass of EPS, as determined by gel permeation chroma-tography, was 203,000. (1)H and (13)C NMR analysis indicated that the identity of EPS may be a glucan. When EPS, starch, and cellulose was treated with a-amylase, glucoamylase, glucosidase, and cellulase, glucose was produced from starch and cellulose but was not produced from EPS. Based on HPLC analysis, elemental analysis, (1)H and (13)C NMR analysis, and enzymatic hydrolysis tests, EPS was estimated to be a glucan. EPS suspension was not precipitated even by centrifugation at 10,000xg for 60 min, and EPS made the fermented milk and bacterial culture viscous.
authors.affiliation: Department of Biological Engineering, Seokyeong University, Seoul 136-704, Republic of Korea.
authors.forename: Min Ju
authors.initials: MJ
authors.lastname: Kim
authors.forename: Ha Na
authors.initials: HN
authors.lastname: Seo
authors.forename: Tae Sik
authors.initials: TS
authors.lastname: Hwang
authors.forename: Sung Hun
authors.initials: SH
authors.lastname: Lee
authors.forename: Doo Hyun
authors.initials: DH
authors.lastname: Park
mesh_terms: D001937:Brassica / Q000378:metabolism / Q000382:microbiology*; D005285:Fermentation; D005516:Food Microbiology; D016937:Gram-Positive Endospore-Forming Rods / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D006868:Hydrolysis; D007723:Korea; D007785:Lactose / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011135:Polysaccharides, Bacterial; D007785:Lactose