Spectrofluorimetric determination of fluoroquinolones in pharmaceutical preparations.
Simple, rapid and highly sensitive spectrofluorimetric method is presented for the determination of four fluoroquinolone (FQ) drugs, ciprofloxacin, enoxacin, norfloxacin and moxifloxacin in pharmaceutical preparations. Proposed method is based on the derivatization of FQ with 4-chloro-7-nitrobenzofurazan (NBD-Cl) in borate buffer of pH 9.0 to yield a yellow product. The optimum experimental conditions have been studied carefully. Beer's law is obeyed over the concentration range of 23.5-500 ng mL(-1) for ciprofloxacin, 28.5-700 ng mL(-1) for enoxacin, 29.5-800 ng mL(-1) for norfloxacin and 33.5-1000 ng mL(-1) for moxifloxacin using NBD-Cl reagent, respectively. The detection limits were found to be 7.0 ng mL(-1) for ciprofloxacin, 8.5 ng mL(-1) for enoxacin, 9.2 ng mL(-1) for norfloxacin and 9.98 ng mL(-1) for moxifloxacin, respectively. Intra-day and inter-day relative standard deviation and relative mean error values at three different concentrations were determined. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed methods. The method is highly sensitive and specific. The results obtained are in good agreement with those obtained by the official and reference method. The results presented in this report show that the applied spectrofluorimetric method is acceptable for the determination of the four FQ in the pharmaceutical preparations. Common excipients used as additives in pharmaceutical preparations do not interfere with the proposed method.
Ulu
Sevgi Tatar
ST
Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, Beyazit, Istanbul, Turkey. sevgitatar@yahoo.com
D009327:4-Chloro-7-nitrobenzofurazan / Q000737:chemistry; D024841:Fluoroquinolones / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013607:Tablets; D013696:Temperature; D013997:Time Factors
D024841:Fluoroquinolones; D004364:Pharmaceutical Preparations; D012997:Solvents; D013607:Tablets; D009327:4-Chloro-7-nitrobenzofurazan