title: Effect of caffeine on the secretion of peroxidase in rat submandibular gland: a study of its mechanism of action.
abstract: OBJECTIVE
  In this work, we analysed the mechanism of action of caffeine on peroxidase secretion in the female rat submandibular gland. The signaling molecules cAMP and nitric oxide were monitored as potential mediators.
  
  
  METHODS
  The salivary gland peroxidase secretion of female albino Wistar rats was assessed by a spectroscopic method.
  
  
  RESULTS
  Caffeine was found to exert an increase on peroxidase secretion in a concentration-response manner: the peroxidase secretion stimulation index (SI) (secreted peroxidase from treated/secreted peroxidase from basal) for caffeine 10 microg/ml: 2.2+/-0.18 (P<0.05); caffeine 100 microg/ml alone: 3+/-0.18 (P<0.01); +LNMMA (LN monomethyl arginine): 1+/-0.1 (P<0.05); caffeine 1000 microg/ml alone: 5+/-0.35 (P<0.01); +LNMMA: 2+/-0.2 (P<0.05). These results were associated with an increase in cAMP and total nitrites production. Total nitrites, SI caffeine 100 microg/ml alone: 2.8+/-0.2 (P<0.01); +LNMMA: 1+/-0.08 (P<0.05); caffeine 1000 microg/ml alone: 4.8+/-0.3 (P<0.01); +LNMMA: 2.3+/-0.18 (P<0.05).
  
  
  CONCLUSIONS
  It could thus be concluded that cAMP and NO are involved in the mechanism of action displayed by caffeine. This is the first report on the mechanism of action of caffeine on peroxidase secretion.
authors.affiliation: IQUIMEFA (UBA-CONICET), Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 2 do Piso, (1113) Buenos Aires, Argentina.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Turner
authors.forename: Maria Gabriela
authors.initials: MG
authors.lastname: Manuele
authors.forename: Roberto
authors.initials: R
authors.lastname: Davicino
authors.forename: Graciela
authors.initials: G
authors.lastname: Ferraro
authors.forename: Rosana
authors.initials: R
authors.lastname: Filip
authors.forename: Claudia
authors.initials: C
authors.lastname: Anesini
mesh_terms: D000818:Animals; D002110:Caffeine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000242:Cyclic AMP / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D007093:Imidazoles / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000502:physiology; D009573:Nitrites / Q000378:metabolism; D009924:Organ Culture Techniques; D009195:Peroxidase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D007093:Imidazoles; D009573:Nitrites; D019323:omega-N-Methylarginine; D002110:Caffeine; C029899:imidazole; D000242:Cyclic AMP; D009195:Peroxidase; D019001:Nitric Oxide Synthase