The effects of lactoferrin in a rat model of catecholamine cardiotoxicity.
Lactoferrin is recently under intense investigation because of its proposed several pharmacologically positive effects. Based on its iron-binding properties and its physiological presence in the human body, it may have a significant impact on pathological conditions associated with iron-catalysed reactive oxygen species (ROS). Its effect on a catecholamine model of myocardial injury, which shares several pathophysiological features with acute myocardial infarction (AMI) in humans, was examined. Male Wistar rats were randomly divided into four groups according to the received medication: control (saline), isoprenaline (ISO, 100 mg kg(-1) s.c.), bovine lactoferrin (La, 50 mg kg(-1) i.v.) or a combination of La + ISO in the above-mentioned doses. After 24 h, haemodynamic functional parameters were measured, a sample of blood was withdrawn and the heart was removed for analysis of various parameters. Lactoferrin premedication reduced some impairment caused by ISO (e.g. a stroke volume decrease, an increase in peripheral resistance and calcium overload). These positive effects were likely to have been mediated by the positive inotropic effect of lactoferrin and by inhibition of ROS formation due to chelation of free iron. The failure of lactoferrin to provide higher protection seems to be associated with the complexity of catecholamine cardiotoxicity and with its hydrophilic character.
Mladenka
Premysl
P
Faculty of Pharmacy in Hradec Králové, Department of Pharmacology and Toxicology, Charles University in Prague, Heyrovského 1203, 500 05, Hradec Kralove, Czech Republic. mladenkap@faf.cuni.cz
Semecký
Vladimír
V
Bobrovová
Zuzana
Z
Nachtigal
Petr
P
Vávrová
Jaroslava
J
Holecková
Magdaléna
M
Palicka
Vladimir
V
Mazurová
Yvona
Y
Hrdina
Radomír
R
D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002614:Chelating Agents / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006439:Hemodynamics; D007501:Iron / Q000737:chemistry; D007781:Lactoferrin / Q000737:chemistry*; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species
D002395:Catecholamines; D002614:Chelating Agents; D017382:Reactive Oxygen Species; D007501:Iron; D005979:Glutathione Peroxidase; D007781:Lactoferrin; D005978:Glutathione