title: A decreased positivity for CD90 on human mesenchymal stromal cells (MSCs) is associated with a loss of immunosuppressive activity by MSCs.
abstract: Biologic and clinical interest in human mesenchymal stromal cells (hMSC) has risen over the last years, mainly due to their immunosuppressive properties. In this study, we investigated the basis of immunomodulant possible variability using hMSC from different sources (amniotic membrane, chorion, and bone marrow from either healthy subjects or patients with hematological malignancies, HM) and having discordant positivity for several immunological markers. The CD90+ hMSC reduced lymphoproliferative response in phytohemagglutinin (PHA) activated peripheral blood mononuclear cells (PBMC) via sHLA-G and IL-10 up-modulation. On the contrary, hMSC showing a significantly lower expression for CD90 antigen, elicited a lymphoproliferative allogeneic response in PHA/PBMCs without any increase in soluble HLA-G and IL-10 levels. These data seems to suggest that CD90 molecule may be considered a novel predictive marker for hMSC inhibitory ability, and might cooperate with HLA-G molecule in regulating suppressive versus stimulatory properties of hMSC. These results may have clinical implication in either transplantation or in regenerative medicine fields.
authors.affiliation: Hematology Section, University, St. Anna Hospital, Corso Giovecca n. 203, Ferrara, Italy.
authors.forename: Diana
authors.initials: D
authors.lastname: Campioni
authors.forename: Roberta
authors.initials: R
authors.lastname: Rizzo
authors.forename: Marina
authors.initials: M
authors.lastname: Stignani
authors.forename: Loredana
authors.initials: L
authors.lastname: Melchiorri
authors.forename: Luisa
authors.initials: L
authors.lastname: Ferrari
authors.forename: Sabrina
authors.initials: S
authors.lastname: Moretti
authors.forename: Antonio
authors.initials: A
authors.lastname: Russo
authors.forename: Gian Paolo
authors.initials: GP
authors.lastname: Bagnara
authors.forename: Laura
authors.initials: L
authors.lastname: Bonsi
authors.forename: Francesco
authors.initials: F
authors.lastname: Alviano
authors.forename: Giacomo
authors.initials: G
authors.lastname: Lanzoni
authors.forename: Antonio
authors.initials: A
authors.lastname: Cuneo
authors.forename: Olavio R
authors.initials: OR
authors.lastname: Baricordi
authors.forename: Francesco
authors.initials: F
authors.lastname: Lanza
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D005434:Flow Cytometry; D006680:HLA Antigens / Q000276:immunology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000378:metabolism*; D008875:Middle Aged; D018800:Thy-1 Antigens / Q000032:analysis* / Q000276:immunology / Q000378:metabolism*
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D018800:Thy-1 Antigens