Differential pulse anodic stripping voltammetry detection of metallothionein at bismuth film electrodes.
As a representation of metalloproteins, metallothionein (MT), which plays important biological and environmental roles such as in the metabolism and detoxification of some metals, was detected at bismuth film electrode (BiFE) by differential pulse anodic stripping voltammetry (DPASV). In pH 2-5.5, two well-defined anodic peaks were produced and attributed to the Zn(2+) and Cd(2+) inherent to MT. The calibration plot of DPASV peak currents for Cd(2+) inherent to MT versus MT concentrations showed a good linearity with a detection limit of 3.86 x 10(-8)mol/L for MT. As a non-toxic excellent electrode material, BiFE shows good performance for detecting MT, and is expected to find further applications in the studies of many other metalloproteins.
Yang
Minli
M
Department of Chemistry, Key Lab. of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, China.
Zhang
Zhanjun
Z
Hu
Zhongbo
Z
Li
Jinghong
J