Molecular identity of uncoupling proteins in thermogenic skunk cabbage.
Thermogenic skunk cabbage has been reported to have two types of uncoupling protein (UCP), a typical 6-transmembrane (TM) SrUCPA and an atypical 5-TM SrUCPB. To verify further the role of SrUCPs in thermogenic skunk cabbage, we examined the molecular identity of SrUCPs in more detail. Both mRNA and genomic analyses supported the presence of SrUCPA, but not SrUCPB. Furthermore, SrUCP protein purified from spadix mitochondria was identified as SrUCPA by mass spectrometry. These results clearly indicate that SrUCPA is the major expressed UCP in skunk cabbage, and the presence of atypical SrUCPB is unlikely to be associated with thermogenesis of skunk cabbage.
Ito-Inaba
Yasuko
Y
Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University, Morioka, 020-8550 Japan. ykoito@iwate-u.ac.jp
Hida
Yamato
Y
Mori
Hitoshi
H
Inaba
Takehito
T
D000595:Amino Acid Sequence; D029064:Araceae / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D013696:Temperature; D000071256:Uncoupling Protein 1
D007473:Ion Channels; D024101:Mitochondrial Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D000071256:Uncoupling Protein 1