[Fluorescein transport and antioxidant systems in the yeast Saccharomyces cerevisiae under acid stress].
The influence of acetic acid induced stress on the activity of fluorescein extrusion system and cell survival in the yeast Saccharomyces cerevisiae has been studied. It was shown that acetic acid caused the inhibition of fluorescein efflux from the cells of both parental strain and its derivative defective in the transcriptional factor War1 which regulates the system of acetate efflux from the cell. The stress induced by 200 mM CH3COOH decreased almost 10 times the survival of strains deficient in the regulatory proteins War1 and Yap1 as compared with respective wild strains. However, pretreatment of the yeast by sublethal concentrations of hydrogen peroxide resulted in the increased resistance to acid stress. Thus it may be supposed that several systems exist which are responsible for acetate extrusion from the yeast cells. Regulatory proteins War1 and Yap1 are involved in the yeast adaptation to the stress induced by acetic acid.
Abrat
O B
OB
Semchyshyn
H M
HM
Miedzobrodski
J
J
Lushchak
V I
VI
D019342:Acetic Acid / Q000494:pharmacology*; D000222:Adaptation, Physiological / Q000187:drug effects; D001692:Biological Transport / Q000187:drug effects; D019793:Fluorescein / Q000493:pharmacokinetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009154:Mutation; D018384:Oxidative Stress / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C472674:War1 protein, S cerevisiae; C086677:YAP1 protein, S cerevisiae; D006861:Hydrogen Peroxide; D019342:Acetic Acid; D019793:Fluorescein