title: Multiclass analysis of illicit drugs in plasma and oral fluids by LC-MS/MS.
abstract: An analytical procedure for the simultaneous determination in human plasma and oral fluids of several illicit drugs belonging to different chemical and toxicological classes is presented. Amphetamine, methamphetamine, morphine, 6-monoacetylmorphine, methylenedioxyamphetamine, methylenedioxyethylamphetamine, methylenedioxymethamphetamine, cocaine, benzoylecgonine, tetrahydrocannabinol, carboxytetrahydrocannabinol, ketamine, and phencyclidine have been quantified in real samples using a very rapid sample treatment, basically a protein precipitation. The quantitative analysis was performed by liquid chromatography-tandem mass spectrometry and has been fully validated. All the analytes were detected in positive ionization mode using a TurboIonSpray source, except carboxytetrahydrocannabinol, which was detected in negative ionization mode. The use of a diverter valve between the column and the mass spectrometer allows the preservation of the ion source performances for high-throughput analysis.
authors.affiliation: Department of Food Science, University of Teramo, Via C. R. Lerici 1, Mosciano S.A., 64023, Teramo, Italy. msergi@unite.it
authors.forename: M
authors.initials: M
authors.lastname: Sergi
authors.forename: E
authors.initials: E
authors.lastname: Bafile
authors.forename: D
authors.initials: D
authors.lastname: Compagnone
authors.forename: R
authors.initials: R
authors.lastname: Curini
authors.forename: G
authors.initials: G
authors.lastname: D'Ascenzo
authors.forename: F S
authors.initials: FS
authors.lastname: Romolo
mesh_terms: D015104:3,4-Methylenedioxyamphetamine / Q000097:blood; D000661:Amphetamine / Q000097:blood; D001826:Body Fluids / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003042:Cocaine / Q000031:analogs & derivatives / Q000097:blood; D013759:Dronabinol / Q000031:analogs & derivatives / Q000097:blood; D006801:Humans; D013287:Illicit Drugs / Q000097:blood*; D007649:Ketamine / Q000097:blood; D008694:Methamphetamine / Q000097:blood; D009020:Morphine / Q000097:blood; D009022:Morphine Derivatives / Q000097:blood; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000097:blood; D010622:Phencyclidine / Q000097:blood; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D013287:Illicit Drugs; D009022:Morphine Derivatives; D008694:Methamphetamine; D015104:3,4-Methylenedioxyamphetamine; C016780:11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid; C005618:benzoylecgonine; D007649:Ketamine; D009020:Morphine; D013759:Dronabinol; D000661:Amphetamine; D003042:Cocaine; D010622:Phencyclidine; D018817:N-Methyl-3,4-methylenedioxyamphetamine; C026979:6-O-monoacetylmorphine