Consumption of oat beta-glucan with or without plant stanols did not influence inflammatory markers in hypercholesterolemic subjects.
We have earlier demonstrated that muesli enriched with oat beta-glucan effectively lowered serum LDL cholesterol. Addition of plant stanols further lowered LDL cholesterol. Besides these hypocholesterolemic effects, beta-glucan and plant stanol esters (PSE) may also affect inflammatory processes. Forty-two mildly hypercholesterolemic subjects randomly consumed for 4 wk (crossover design) control muesli (4.8 g control fiber), beta-glucan muesli (4.8 g oat beta-glucan), or combination muesli (4.8 g oat beta-glucan plus 1.4 g stanol as PSE). Changes in cytokine production (IL-6, IL-8, and TNF-alpha) of LPS-stimulated peripheral blood mononuclear cells (PBMC) and whole blood were evaluated, as well as changes in plasma high-sensitivity (hs)-CRP. Additionally, changes in expression profiles of 84 genes involved in atherosclerosis metabolism were assessed in isolated PBMC. IL-6, IL-8, and TNF-alpha production by PBMC and whole blood after LPS stimulation did not differ between the treatments. Also high-sensitivity C-reactive protein (hs-CRP) levels were similar. beta-Glucan consumption did not change gene expression, while only 3 genes (ADFP, CDH5, CSF2) out of the 84 genes from the atherosclerotic risk panel were differentially expressed (p < 0.05) after consumption of PSE. Consumption of beta-glucan with or without PSE did not influence inflammatory parameters in mildly hypercholesterolemic subjects.
Theuwissen
Elke
E
Department of Human Biology, Maastricht University, Maastricht, The Netherlands.
Plat
Jogchum
J
Mensink
Ronald P
RP
D000328:Adult; D050197:Atherosclerosis / Q000235:genetics; D018554:Avena / Q000737:chemistry*; D002097:C-Reactive Protein / Q000032:analysis; D018592:Cross-Over Studies; D004043:Dietary Fiber / Q000008:administration & dosage; D004311:Double-Blind Method; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020022:Genetic Predisposition to Disease; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D007249:Inflammation / Q000097:blood*; D007375:Interleukin-1 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D012855:Sitosterols / Q000008:administration & dosage*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D047071:beta-Glucans / Q000008:administration & dosage*
D004043:Dietary Fiber; D007375:Interleukin-1; D016209:Interleukin-8; D008070:Lipopolysaccharides; D012855:Sitosterols; D014409:Tumor Necrosis Factor-alpha; D047071:beta-Glucans; C407042:plant stanol ester; D002097:C-Reactive Protein