Determination of bromazepam by coupling a continuous liquid-liquid extractor to an atomic-absorption spectrometer.
Bromazepam, in the form of the ion-pair (bromazepam)(3) Cu(ClO(4))(2), can be extracted into methyl isobutyl ketone, and determined indirectly by atomic-absorption spectrometry with a limit of detection of 0.1 mug/ml bromazepam at the 324.8 nm copper resonance line. The optimum conditions for determining this drug (0.4-4.0 mug/ml) are described. The relative standard deviation achieved is 1.7%. The method is selective and free from the interference of other 1,4-benzodiazepines. It was applied to the determination of bromazepam in pharmaceuticals and was also spiked in plasma samples.
Santelli
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Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Cordoba-14004, Spain.
Gallego
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Valcarcel
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