title: Proteomic analysis of Latrodectus tredecimguttatus venom for uncovering potential latrodectism-related proteins.
abstract: Black widow spider is one of the most poisonous spiders in the world. Up to now, there have been few systematic analyses of the spider venom components, and the mechanism of action of the venom has not been completely understood. In this work, we employed combinative proteomic strategy to analyze the venom collected from living adult spider Latrodectus tredecimguttatus by electrical stimulation. The experiments demonstrated that the venom is primarily composed of high molecular weight proteins and has high abundance proteins around 100 kDa. The content of peptides and proteins with low molecular weight is low. A total of 75 nonredundant venom proteins with distinct function were unambiguously identified. Besides the known black widow spider venom proteins including latrotoxins, a variety of hydrolases and other proteins with special activity were found in the venom, such as proteinase, phospholipase, phosphatase, nuclease, fucolectin, venom allergen antigen 5-like protein and trypsin inhibitor, and so on. Their possible biological actions and relationship with latrodectism were discussed. The results help to understand the complexity and action mechanism of L. tredecimguttatus venom.
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, People's Republic of China.
authors.forename: Zhigui
authors.initials: Z
authors.lastname: Duan
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yan
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Xianchun
authors.initials: X
authors.lastname: Wang
authors.forename: Songping
authors.initials: S
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001740:Black Widow Spider / Q000737:chemistry*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D040901:Proteomics; D013111:Spider Venoms / Q000032:analysis* / Q000737:chemistry
chemical_list: D010455:Peptides; D011506:Proteins; D013111:Spider Venoms; C038599:black widow spider venom