Ferrocene-mannose conjugates as electrochemical molecular sensors for concanavalin A lectin.
The binding affinity of a series of electroactive glycoconjugates, based on a ferrocene core bearing alpha-mannose units on one or both of its cyclopentadienyl rings, to lectin Con A was studied by isothermal titration calorimetry (ITC) and voltammetry. Voltammetric measurements were performed by differential pulse adsorptive stripping voltammetry (DPAdSV). Upon complexation of ferrocene-mannose conjugates with Con A, voltammograms showed a decrease of the peak current. Both the monomannosylated ferrocene and the bis(mannosylated) ferrocene derivatives form more stable complexes with Con A than methyl alpha-D-mannopyranoside. Bis(mannosylated) ferrocene conjugates were found to bind to Con A with enhanced affinity due to the multivalent effect. A comparison of the thermodynamic data obtained by ITC and voltammetry is presented.
Casas-Solvas
Juan M
JM
Area de Química Orgánica, Universidad de Almería, Crta. de Sacramento, s/n, 04120, Almería, Spain.
Ortiz-Salmerón
Emilia
E
García-Fuentes
Luís
L
Vargas-Berenguel
Antonio
A
D002151:Calorimetry; D003208:Concanavalin A / Q000032:analysis / Q000378:metabolism*; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D000075163:Metallocenes
D005296:Ferrous Compounds; D000075163:Metallocenes; D003208:Concanavalin A; D008358:Mannose; C004998:ferrocene