Cyclic peptides from Oldenlandia affinis DC. Molecular and biological properties.
A new isolation procedure for Kalata polypeptides from the tropical plant Oldenlandia affinis DC is described. Fractions were screened by thin-layer chromatography, and Van Urk positive fractions were tested for oxytocic activity in estrogenized rat uteri. By using this procedure, we were able to isolate and characterize three macrocyclic polypeptides with uterine activity. Their amino acid sequence and biological effects have been analyzed, and their NMR spectra were compared with those of the earlier ones. All three peptides showed hemolytic activity on human blood, and were tested for antibiotic effect against E. coli, Staphylococcus aureus, and Hemophilus influenzae.
Gran
Lorents
L
Department of Biochemistry/Biotechnology Center of Oslo, University of Oslo, N-0316 Oslo.
Sletten
Knut
K
Department of Biochemistry/Biotechnology Center of Oslo, University of Oslo, N-0316 Oslo.
Skjeldal
Lars
L
IKBM, Norwegian University of Life Sciences, N-1432 Ås.
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005260:Female; D006193:Haemophilus influenzae / Q000187:drug effects / Q000254:growth & development; D006461:Hemolysis / Q000187:drug effects; D053878:Hemolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D032067:Oldenlandia / Q000737:chemistry*; D010120:Oxytocics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011487:Protein Conformation; D051381:Rats; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D014590:Uterine Contraction / Q000187:drug effects; D014599:Uterus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D053878:Hemolytic Agents; D010120:Oxytocics; D010456:Peptides, Cyclic