New strategy for quantifying biological zinc by a modified zinpyr fluorescence sensor.
Substitution of one pyridine by pyrazine in each DPA appendage of ZP1 leads to a new zinc sensor, ZPP1, with a modified background fluorescence and zinc affinity. ZPP1 exhibits a two-step zinc response during fluorescence titrations, which leads to a new method for zinc quantification. The ability of ZPP1 to image and quantify zinc was demonstrated in pancreatic Min6 cells.
Zhang
Xiao-an
XA
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Hayes
Dugan
D
Smith
Sarah J
SJ
Friedle
Simone
S
Lippard
Stephen J
SJ
D002460:Cell Line; D002470:Cell Survival; D005452:Fluoresceins / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000032:analysis* / Q000737:chemistry*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D015032:Zinc; C501987:zinpyr-1