Screening of biomarker genes activated by irradiation of ultraviolet B rays in mouse lymph node M10 cells.
When M10 cells derived from mouse lymph nodes were irradiated with the UVB lamp at a peak emission of 312 nm, the cell growth was suppressed in proportion to irradiation time (10-30 s) and cell apoptosis was also induced by the irradiation. Dynamic changes in 597 genes after exposing these cells to UVB irradiation were investigated by DNA array analysis using array membranes and a (33)P-labeling probe. After 2 h of irradiation, the gene expression in the cells was examined and compared with that in untreated cells. Radioactivity was analyzed using Array Gauge software. The data were further processed using software, EX-ARRAY, which was developed for extracting significant data from the results of 2 background-subtraction methods, i.e., global and local background subtraction. The number of genes suppressed under UV irradiation increased with irradiation time, while that of activated genes decreased. Finally, we confirmed 4 genes (HMG-14, CDX-2, MCP-3, and GRP-78) to be up-regulated and confirmed their activation by northern blot. We propose these genes as the new biomarkers of lymphocyte sensitive to UVB irradiation.
Niimura
Yukio
Y
Research Center of Biomedical Analysis and Radioisotope, Faculty of Medicine, Teikyo University, Tokyo, Japan. yniimura@med.teikyo-u.ac.jp
Moue
Toshiko
T
Takahashi
Nobuyoshi
N
Nagai
Ken-Ichi
K
D000818:Animals; D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D020869:Gene Expression Profiling; D005819:Genetic Markers / Q000235:genetics*; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D051379:Mice; D020543:Proteome / Q000378:metabolism*; D011829:Radiation Dosage; D014466:Ultraviolet Rays
D005819:Genetic Markers; D020543:Proteome