title: Sol-gel based amperometric biosensor incorporating an osmium redox polymer as mediator for detection of L-lactate.
abstract: A novel amperometric biosensor for the determination of lactate was constructed by first immobilizing lactate oxidase and an osmium redox polymer ([Os(bpy)(2)(PVP)(10)Cl]Cl; abbreviated Os-polymer) on the surface of a glassy carbon electrode, followed by coating with a sol-gel film derived from methyltriethoxysilane (MTEOS). The electrooxidation current of this electrode was found to be diffusion controlled. In the presence of lactate, a clear electrocatalytic oxidation wave was observed, and lactate could be determined amperometrically at 400 mV versus Ag AgCl . The concentration range of linear response, slope of linear response and detection limit were 0.1-9 mM, 1.02 microA mM(-1), and 0.05 mM, respectively. Although L-ascorbate was electrooxidized at this potential, uric acid, paracetamol and glucose were found not to interfere.
authors.affiliation: BEST Centre, School of Chemical Sciences, Dubin City University, Dublin 9, Ireland.
authors.forename: T M
authors.initials: TM
authors.lastname: Park
authors.forename: E I
authors.initials: EI
authors.lastname: Iwuoha
authors.forename: M R
authors.initials: MR
authors.lastname: Smyth
authors.forename: R
authors.initials: R
authors.lastname: Freaney
authors.forename: A J
authors.initials: AJ
authors.lastname: McShane