In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in rats.
This study investigated the in vivo effects of the Bothrops jararaca venom (BjV) on general metabolic profile and, specifically, on muscle protein metabolism in rats. The crude venom (0.4 mg/kg body weight, IV) was infused in awake rats, and plasma activity of enzymes and metabolites levels were determined after 1, 2, 3, and 4 hours. BjV increased urea, lactate, and activities of creatine kinase, lactate dehydrogenase, and aspartate aminotransferase after 4 hours. The content of liver glycogen was reduced by BjV. Protein metabolism was evaluated by means of microdialysis technique and in isolated muscles. BjV induced increase in the muscle interstitial-arterial tyrosine concentration difference, indicating a high protein catabolism. The myotoxicity induced by this venom is associated with reduction of protein synthesis and increase in rates of overall proteolysis, which was accompanied by activation of lysosomal and ubiquitin-proteasome systems without changes in protein levels of cathepsins and ubiquitin-protein conjugates.
Gonçalves
Dawit A P
DA
Department of Molecular Biology and Division of Nephrology, School of Medicine of São José do Rio Preto, São Paulo, Brazil. dawitapg@yahoo.com.br
Silva
Erico V C M
EV
Graça
Flávia A
FA
Lira
Eduardo C
EC
Zanon
Neusa M
NM
Mendes
Glória E
GE
Burdmann
Emmanuel A
EA
Migliorini
Renato H
RH
Kettelhut
Isis C
IC
Navegantes
Luiz C C
LC
D000818:Animals; D001794:Blood Pressure; D017837:Bothrops; D003435:Crotalid Venoms / Q000494:pharmacology*; D006868:Hydrolysis; D050356:Lipid Metabolism; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow
D003435:Crotalid Venoms; D008112:Liver Glycogen; D009124:Muscle Proteins