[Interaction between 5-lipoxygenase and allosteric effector--sodium dodecyl sulfate].
The role of allosteric effector--sodium dodecyl sulfate (SDS) in the lipoxygenase catalysis in micelle system has been studied. The effect of the stable hydrophobic bis-nitroxides, blocking the free radical transformation, on the oxidation of linoleic acid or linoleic alcohol by 5-lipoxygenase from potato tuber has been investigated. The inhibiting effect of nitroxide compounds on oxidation of linoleic acid or linoleic alcohol by 5-lipoxygenase depends on SDS concentration. The inhibition percentage is determined by the substrate nature and presence of allosteric effector. The presence of SDS did not lead to an appreciable change in the pKa values of ionogenic enzyme groups. The effect of SDS and micellar system on thermodynamic parameters for thermoinactivation of 5-lipoxygenase was studied. It was found that thermoinactivation rate constants and activation energy of enzyme thermoinactivation were increased in the presence of SDS. It is suggested that interaction of 5-lipoxygenase and allosteric effector--SDS intensifies the dissociation of radical intermediates from the active site of the enzyme. These findings are of physiological significance in the light of the lipoxygenase involvement in the membrane lipid peroxidation.
Kharytonenko
H I
HI
Skaterna
T D
TD
Mel'nyk
A K
AK
Babiĭ
L V
LV
Kharchenko
O V
OV
D000494:Allosteric Regulation; D001094:Arachidonate 5-Lipoxygenase / Q000737:chemistry* / Q000302:isolation & purification; D015227:Lipid Peroxidation; D008563:Membrane Lipids / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D011198:Solanum tuberosum / Q000201:enzymology; D013379:Substrate Specificity
D008563:Membrane Lipids; D012967:Sodium Dodecyl Sulfate; D001094:Arachidonate 5-Lipoxygenase