Studies of the homogeneous immunochemical determination of insulin by using a fluorescent label.
Fluorescence intensity, lifetime and polarization were investigated for use in the homogeneous immunochemical determination of bovine and human insulin. Both antibody-labelled and antigen-labelled schemes were studied. A competitive, antigen-labelled immunoassay based on the difference in fluorescence polarization between free and antibody-bound fluorescein-labelled insulin was found to be the most sensitive approach. The problem of adsorption of insulin on the cuvette surfaces was studied, and sodium salicylate was used with silica cuvettes to minimize the adsorption. Anti-porcine insulin antibody and fluorescein-labelled bovine insulin were successfully used for the determination of both human insulin and bovine insulin.
Nithipatikom
K
K
Department of Chemistry, P.M. Gross Chemical Laboratory, Duke University, Durham, NC 27706, U.S.A.
McGown
L B
LB