Chiral separation with gradient elution isotachophoresis for future in situ extraterrestrial analysis.
The first results of chiral separations with the gradient elution isotachophoresis method are presented. As previously described, citrate is used in the run buffer as the leading ion and borate in the sample buffer as the terminating ion. Modulation of parameters such as electrolyte pH, pressure scan rate, chiral selector concentration, combinations of CD or the percentage of ampholytes provides an easy optimization of the separations. To perform fluorescent detection 5-carboxyfluorescein succinimidyl ester and two fluorogenic-labeling agents, fluorescamine (Fluram) and 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde, are used to label amino acids. With the 5-carboxyfluorescein amino acids, chiral separations are easily obtained using a neutral CD ((2-hydroxypropyl)-beta-CD) at a low concentration (2 mmol/L). With Fluram amino acids, the situation is more complicated due to the formation of diastereoisomers and due to weak interactions with the different CDs used. The use of the 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde-labeling agent solves the problems observed with the Fluram agent while retaining the fluorogenic properties. These first results demonstrate the simplicity and the feasibility of gradient elution isotachophoresis for chiral separations.
Danger
Grégoire
G
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.
Ross
David
D
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000596:Amino Acids / Q000032:analysis* / Q000302:isolation & purification; D001565:Benzoates / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D005118:Extraterrestrial Environment / Q000737:chemistry*; D005450:Fluorescamine / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D011804:Quinolines / Q000737:chemistry; D015203:Reproducibility of Results; D013026:Space Flight; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry
D000596:Amino Acids; D001565:Benzoates; D005452:Fluoresceins; D011804:Quinolines; D047392:beta-Cyclodextrins; C069617:3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D005450:Fluorescamine