title: Microinjection of A. aegypti embryos to obtain transgenic mosquitoes.
abstract: In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, desiccating embryos, and performing microinjection are demonstrated.
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California, Irvine, USA. njasinsk@uci.edu
authors.forename: Nijole
authors.initials: N
authors.lastname: Jasinskiene
authors.forename: Jennifer
authors.initials: J
authors.lastname: Juhn
authors.forename: Anthony A
authors.initials: AA
authors.lastname: James
mesh_terms: D000330:Aedes / Q000196:embryology* / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D004247:DNA / Q000008:administration & dosage; D003715:Dengue / Q000635:transmission; D018014:Gene Transfer Techniques; D007303:Insect Vectors; D008845:Microinjections / Q000379:methods
chemical_list: D004247:DNA