title: Corticosteroid administration alters the mechanical properties of isolated collagen fascicles in rat-tail tendon.
abstract: Overload tendon injuries are frequent in recreational and elite sports. The optimal treatment strategy remains unknown, but local administration of corticosteroids is one common treatment option. The direct effects of the corticosteroid administration on the tissue are not fully understood. The present study examined the biomechanical effects of intratendinous corticosteroid injections on healthy rat-tail tendon collagen fascicles. A total of 24 Wistar male rats were divided into (A) a corticosteroid group where the animals were injected in the tail tendon with methylprednisolone acetate, 1.0 mL of 40 mg/mL mixed with 1.0 mL 9% saline (n=12), and (B) a control group that was injected with 9% saline (n=12). Three days after the injections, the animals were sacrificed and single individual collagen fascicles were collected and underwent displacement to failure. Corticosteroid administration significantly reduced tensile fascicle yield strength by 16% and Young's modulus by 14% compared with sham treatment (10.5+/-0.8 vs 12.4+/-0.5 MPa, P< or =0.05, and 537+/-27 vs 641+/-30 MPa, P<0.05, respectively), while the strain properties were unaffected. Peak stress was similar between the two groups. There was no difference in fascicle diameter between the two groups.
authors.affiliation: Institute of Sports Medicine Copenhagen, Faculty of Health Sciences, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark.
authors.forename: B T
authors.initials: BT
authors.lastname: Haraldsson
authors.forename: P
authors.initials: P
authors.lastname: Aagaard
authors.forename: D
authors.initials: D
authors.lastname: Crafoord-Larsen
authors.forename: M
authors.initials: M
authors.lastname: Kjaer
authors.forename: S P
authors.initials: SP
authors.lastname: Magnusson
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000378:metabolism*; D001696:Biomechanical Phenomena / Q000187:drug effects; D003094:Collagen / Q000187:drug effects*; D008297:Male; D008775:Methylprednisolone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D000077555:Methylprednisolone Acetate; D051381:Rats; D017208:Rats, Wistar; D013623:Tail / Q000502:physiology*; D013710:Tendons / Q000187:drug effects*; D013718:Tensile Strength / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D000077555:Methylprednisolone Acetate; D003094:Collagen; D008775:Methylprednisolone