title: Spectroscopic analysis of the interaction of a peptide sequence of Hepatitis G virus with bilayers.
abstract: Merocyanine 540 (MC540) has been used as external probe to determine the interaction of the peptide sequence 125-139 corresponding to the E2 protein of Hepatitis G virus, with lipid bilayers. The probe was incorporated into large unilamellar vesicles (LUVs) or small unilamellar vesicles (SUVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). When incorporated into bilayers, MC540 shows two absorption maxima corresponding to the monomer (570 nm) and dimer (530 nm) form of the probe. Changes in the probe microenvironment are reflected by a modification in the position and/or intensity of these maxima. Addition of increasing amounts of peptide resulted in a slight decrease of the ratio A570/A530 thus indicating a change in MC540 partition into the membrane, going from a hydrophobic to a more hydrophilic environment. This effect was concomitant with an increase in dimer formation as stated from the values of the apparent dimerization constant (K(app)) obtained. Fluorescence spectra as well as steady state anisotropy measurements were in agreement with the above results indicating that the peptide was able to relocate the probe and displacing MC540 from its initial location into the bilayer. Results with SUVs or LUVs were similar for what curvature does not seem to play any role on peptide activity. These results reflect the ability of peptide to interact with biomimetic membranes in the lipid head group region.
authors.affiliation: Physical Chemistry Department, Faculty of Pharmacy, Avgda Joan XXIII, s/n 08028 Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Alay
authors.forename: J
authors.initials: J
authors.lastname: Prat
authors.forename: I
authors.initials: I
authors.lastname: Haro
authors.forename: N
authors.initials: N
authors.lastname: Rojo
authors.forename: M A
authors.initials: MA
authors.lastname: Alsina
authors.forename: M A
authors.initials: MA
authors.lastname: Busquets