Immunohistolocalization and gene expression of secretory carbonic anhydrase isoenzyme CA-VI in canine nasal cavity.
Immunolocalization of the secretory form of carbonic anhydrase isoenzyme, CA-VI were studied using a specific canine CA-VI antiserum, and CA-VI mRNA signals were also investigated using the reverse-transcriptase polymerase chain reaction (RT-PCR) in canine nasal mucosal epithelia and glands. Immunoreactivity to CA-VI was positive throughout the mucosal epithelial cells and in the cytoplasm of serous acinar and ductal epithelial cells of the nasal mucosa and glands, including the vestibule of the nose, but the mucous acinar cells of the glands were immunonegative. We detected CA-VI gene transcripts in the same regions as the CA-VI immunoreactivity. The physiological roles of CA-VI in the nasal mucosal epithelium and glands might maintain bicarbonate levels in nasal secretions and protect the mucosa against acid.
Sugiura
Yosuke
Y
Department of Anatomy, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
Ichihara
Nobutsune
N
Nishita
Toshiho
T
Murakami
Masaru
M
Amasaki
Hajime
H
Asari
Masao
M
D000818:Animals; D002256:Carbonic Anhydrases / Q000096:biosynthesis* / Q000235:genetics; D004285:Dogs / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry / Q000662:veterinary; D009296:Nasal Cavity / Q000201:enzymology*; D009297:Nasal Mucosa / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D012333:RNA, Messenger; D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI