Overexpression of HMGA2 in uterine leiomyomas points to its general role for the pathogenesis of the disease.
An overexpression of HMGA2 is supposed to be a key event in the genesis of leiomyoma with chromosomal rearrangements affecting the region 12q14-15 targeting the HMGA2 gene, but gene expression data regarding differences between uterine leiomyomas with and those without 12q14-15 aberrations are insufficient. To address the question whether HMGA2 is only upregulated in the 12q14-15 subgroup, the expression of HMGA2 was analyzed in a comprehensive set of leiomyomas (n = 180) including tumors with 12q14-15 chromosomal aberrations (n = 13) and matching myometrial tissues (n = 51) by quantitative RT-PCR. The highest expression levels for HMGA2 were observed in tumors with rearrangements affecting the region 12q14-15, but although HMGA2 is expressed at lower levels in leiomyomas without such aberrations, the comparison between the expression in myomas and matching myometrial tissues indicates a general upregulation of HMGA2 regardless of the presence or absence of such chromosomal abnormalities. The significant (P < 0.05) overexpression of HMGA2 also in the group of fibroids without chromosomal aberrations of the 12q14-15 region suggests a general role of HMGA2 in the development of the disease.
Klemke
Markus
M
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany.
Meyer
Anke
A
Nezhad
Maliheh Hashemi
MH
Bartnitzke
Sabine
S
Drieschner
Norbert
N
Frantzen
Christiane
C
Schmidt
Ernst Heinrich
EH
Belge
Gazanfer
G
Bullerdiek
Jörn
J
D002869:Chromosome Aberrations; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D025743:HMGA2 Protein / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007889:Leiomyoma / Q000235:genetics* / Q000378:metabolism; D009215:Myometrium / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism; D014599:Uterus / Q000378:metabolism
D025743:HMGA2 Protein