title: [Molecular characters of Centella asiatica found with RAPD technology].
abstract: OBJECTIVE
  To analyze the DNA molecular characters of Centella asiatica with RAPD technology.
  
  
  METHODS
  With the genomic DNA as templates extracted from various source of Centella asiatica samples, optimized RAPD PCR reaction systems had been used. The random promers had been screened to amplify the specific molecular fragments of Centella asiatica.
  
  
  RESULTS
  The specific genetic bands of Centella asiatica species from various habitats were established which were highly stable and repeatable and obviously different from those of other families, genuses of plants such as Gynostemma pentaphylum, Tobacco, Cayratia japonica.
  
  
  CONCLUSIONS
  The developed method of RAPD analysis for the genetic character bands of Centella asiatica could be applied to identify real Centella asiatica from its spurious breed plants. The genetic character bands of Centella asiatica amplified with the RAPD method show high homogeneous in several samples from different habitats.
authors.affiliation: Gung Medical University, Nanning 530021, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ruan
authors.forename: Rong-Hao
authors.initials: RH
authors.lastname: Mo
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Liu-Qing
authors.initials: LQ
authors.lastname: Huang
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
authors.forename: Xiong-Ying
authors.initials: XY
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.forename: Yao-Sheng
authors.initials: YS
authors.lastname: Wu
mesh_terms: D028041:Centella / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D004340:Drug Contamination; D005819:Genetic Markers; D018745:Genome, Plant; D029774:Gynostemma / Q000145:classification / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers