title: Flavonoid constituents from Spiranthes australis LINDL.
abstract: Chemical investigation of the whole plant of Spiranthes australis (R. BROWN) LINDL. resulted in the isolation and characterization of three new flavonoid constituents, 5-hydroxy-4'-[(2-isopentenyl)oxy]-3,7,3'-trimethoxyflavone (1), 3-O-[[O-beta-D-xylopyranosyl]-(1-->2)-beta-D-glucopyranosyl]-8-(p-hydroxy-benzyl)-kaempferol (2) and 3-O-[O-[2-O-(E)-p-coumaroyl-beta-D-xylopyranosyl]-(1-->2)-beta-D-glucopyranosyl]-8-(p-hydroxy-benzyl)-kaempferol (3), together with six known flavonoid compounds. The structures of new compounds were determined on the basis of spectroscopic analysis, including HR-ESI-MS, 1D- and 2D-NMR techniques and chemical methods.
authors.affiliation: School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing, PR China.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Dong
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Zhou
mesh_terms: D045744:Cell Line, Tumor; D002855:Chromatography, Thin Layer; D004121:Dimethyl Sulfoxide; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D029595:Orchidaceae / Q000737:chemistry*; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D005419:Flavonoids; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D004121:Dimethyl Sulfoxide