Protective effects of vitamin E against 3,3',4,4',5-pentachlorobiphenyl (PCB126) induced toxicity in zebrafish embryos.
3,3',4,4',5-Pentachlorinated biphenyls 126 (PCB126) is a global environmental contaminant that can induce cellular oxidative stress. We investigated whether vitamin E can protect against toxicity from PCB126 during zebrafish (Danio rerio) development. Zebrafish embryos were exposed to 100nM PCB126 and compared with a second group that was co-exposed with 100muM vitamin E until 5 days post fertilization. PCB126 induced pericardial sac edema, yolk sac edema, and growth retardation in zebrafish embyos. In contrast, vitamin E co-exposure group did not show any gross changes. Real-time PCR results showed that vitamin E co-exposure group were restored to control group for the expression levels of heat shock protein 70 Cognate, aryl hydrocarbon receptor type-2, cytochrome P450 1A, and superoxide dismutase-1. These data give insights into the use of vitamin E to reduce PCB126-mediated toxicity and into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of AHR agonists.
Na
Yi-Rang
YR
Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, Seoul 151-742, Republic of Korea.
Seok
Seung-Hyeok
SH
Baek
Min-Won
MW
Lee
Hui-Young
HY
Kim
Dong-Jae
DJ
Park
Sung-Hoon
SH
Lee
Hyun-Kyoung
HK
Park
Jae-Hak
JH
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004336:Drug Antagonism; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D047108:Embryonic Development / Q000187:drug effects; D004965:Estrogen Antagonists / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010496:Pericardium / Q000187:drug effects / Q000473:pathology; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015017:Yolk Sac / Q000187:drug effects / Q000473:pathology; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
D000975:Antioxidants; D004965:Estrogen Antagonists; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins; D014810:Vitamin E; D011078:Polychlorinated Biphenyls; C023035:3,4,5,3',4'-pentachlorobiphenyl