Cytotoxicity and L-amino acid oxidase activity of animal venoms.
The cytotoxicity of animal venoms (snakes, insects and marine animals) was measured against SNU-1 (stomach cancer cells) by dye uptake assay (MTT method). And also L-amino acid oxidase (AAO) activity of the venoms was compared. Among them, the venom fromOphiophagus hannah (king cobra) showed a strong AAO activity as well as a high potent cytotoxicity. Cytotoxic protein having a AAO was then partially purified by HPLC-GPC and two fractions (Fr. I and Fr. II) were collected. The IC(50) values of Fr. I and Fr. II were 0.19 mug/ml and 1.36 mug/ml, respectively. The results suggested that the cytotoxicity of king cobra venom may be due to its AAO activity.
Ahn
M Y
MY
Natural Products Research Institute, Seoul National University, 28 Yeonkun-Dong, Jongro-Ku, 110-460, Seoul, Korea.
Lee
B M
BM
Kim
Y S
YS