title: Fluorescence resonance energy transfer in doubly-quantum dot labeled IgG system.
abstract: The mouse immunoglobulin G (mouse IgG) as a kind of bio-molecule was labeled with two different luminescent colloidal semiconductor quantum dots (QDs), green-emitting CdTe quantum dots and red-emitting CdTe quantum dots in this work. As a result of the fluorescence resonance energy transfer (FRET) between the two different sizes nanoparticles with mouse IgG as the binding bridge, a significant enhancement of the emission of the red-emitting CdTe quantum dots and the corresponding quenching of the emission of green-emitting CdTe quantum dots were observed. The relationship between the concentration of the mouse immunoglobulin G and the fluorescence intensity ratio (I(a)/I(d)) of acceptors and donors was studied also. Under optimal conditions, the calibration graph is linear over the range of 0.1-20.0mg/L mouse IgG.
authors.affiliation: Institute for Miniature Analytical Instrumentation, College of Chemistry, Jilin University, Changchun 130023, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.forename: Xing-Guang
authors.initials: XG
authors.lastname: Su
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wan
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Wang
authors.forename: Bai
authors.initials: B
authors.lastname: Yang
authors.forename: Qin-Han
authors.initials: QH
authors.lastname: Jin