title: Protocol for dengue infections in mosquitoes (A. aegypti) and infection phenotype determination.
abstract: The purpose of this procedure is to infect the Aedes mosquito with dengue virus in a laboratory condition and examine the infection level and dynamic of the virus in the mosquito tissues. This protocol is routinely used for studying mosquito-virus interactions, especially for identification of novel host factors that are able to determine vector competence. The entire experiment must be conducted in a BSL2 laboratory. Similar to Plasmodium falciparum infections, proper attire including gloves and lab coat must be worn at all times. After the experiment, all the materials that came in contact with the virus need to be treated with 75% ethanol and bleached before proceeding with normal washing. All other materials need to be autoclaved before discarding them.
authors.affiliation: Malaria Research Institute, Bloomberg School of Public Health, John Hopkins University, USA.
authors.forename: Suchismita
authors.initials: S
authors.lastname: Das
authors.forename: Lindsey
authors.initials: L
authors.lastname: Garver
authors.forename: Jose Ruiz
authors.initials: JR
authors.lastname: Ramirez
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Xi
authors.forename: George
authors.initials: G
authors.lastname: Dimopoulos
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D019411:Clinical Laboratory Techniques; D003666:Decontamination; D003715:Dengue / Q000235:genetics* / Q000503:physiopathology*; D003716:Dengue Virus / Q000502:physiology; D017744:Medical Waste Disposal; D010641:Phenotype; D011481:Protective Clothing
chemical_list: D017744:Medical Waste Disposal