Non-equilibrium determination of metoclopramide and tetracaine hydrochloride by sequential injection spectrophotometry.
A new sequential injection spectrophotometric method was proposed for the determination of metoclopramide and tetracaine hydrochloride. The method was based on the detection of an unstable red intermediate compound resulting from the reaction of metoclopramide or tetracaine hydrochloride with potassium dichromate, in the presence of sodium oxalate, in sulfuric acid solution. The related reaction mechanisms of this new method have been studied. The experimental conditions were optimized for the stopped-flow and continuous-flow sequential injection models. For continuous flow, the linear range for determination of metoclopramide, the detection limit and the sampling frequency were 13-130 microgml(-1), 9.4 microgml(-1) and 40 samples per hour, respectively. For stopped flow, they were 3-42 microgml(-1), 1.0 microgml(-1) and 18h(-1), respectively. Adopting the continuous-flow model for tetracaine hydrochloride, the linear range was 25-300 microgml(-1), and the detection limit was 18.0 microgml(-1) with sampling frequency of 40h(-1). This method has been used to determine metoclopramide and tetracaine hydrochloride in pharmaceutical preparations, and the results are compared with those determined by the pharmacopoeia method. Statistical analysis reveals that there was no evidence of significant difference between the methods.
Fan
Jing
J
Henan Key Laboratory for Environmental Pollution Control, School of Chemical and Environmental Sciences, Henan Normal University, Xinxiang 453007, PR China. fanjing168@126.com
Wang
Aijun
A
Feng
Suling
S
Wang
Jianji
J