Chemiluminometric method for the determination of glycerol in wine by flow-injection analysis with co-immobilized glycerol dehydrogenase/NADH oxidase.
A flow-injection method for the determination of glycerol in wine is described. Glycerol dehydrogenase and NADH oxidase were co-immobilized on poly (vinyl alcohol) beads and incorporated in a flow-injection system. The hydrogen peroxide produced was detected chemiluminometrically via a luminol-hexacyanoferrate (III) reaction. Wine was diluted 1000-fold with water and sample solution (50 microl) was injected into the carrier stream. The calibration graph was linear in the range 3 x 10(-7)-3 x 10(-4) M; the detection limit was 7 x 10(-8) M and the sample throughout was 30 h(-1) without carryover.
Kiba
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Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu, Japan.
Azuma
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Furusawa
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