New quantitative determination of 2,3-dioxobutane (diacetyl) in alcoholic beverages by an indirect spectrofluorimetric procedure with use of 2,3-diaminonaphtalene as reagent of derivatization.
The experimental conditions of an earlier spectrofluorimetric determination of 2,3-dioxobutane as 2,3-diaminonaphtalene derivative have been optimized. Fluorescence intensities are increased by a factor of 13 over the precedent ones. The calibration graphs are linear in the concentration range 2.5-130 mumol/l in spectrophotometry and 1.75 x 10(-1)-2.5 mumol/l in fluorimetry. Limits of detection are very small: 7 x 10(-1) and 2.5 x 10(-2) mumol/l, respectively. Applications to alcoholic beverages are described. The new fluorimetric technique is simple and rapid since it does not require steam distillation.
Mariaud
M
M
Laboratoire de Chimie Analytique, Faculté des Sciences Pharmaceutiques, 2 Bis Boulevard Tonnellé, 37042 Tours Cedex, France.
Levillain
P
P