Spectrophotometric determination of epinephrine and norepinephrine with sodium periodate.
A simple and accurate spectrophotometric method is described for the determination of epinephrine (EP), norepinephrine (NE) and their bitartrate salts. The method is based on the development of a red colour (lambda(max) 490 nm) with sodium periodate in aqueous alcoholic medium. The colour is stable for at least 1 hr. The molar reacting ratio of EP or NE to periodate is 1:2. The proposed method is particularly suitable for routine analysis of EP and NE injections. The interference due to the sodium metabisulphite normally used as antioxidant can be overcome by addition of acetone. Results for analysis of bulk drugs and injections agree well with those of official methods.
El-Kommos
M E
ME
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
Mohamed
F A
FA
Khedr
A S
AS