Separation and simultaneous determination of cobalt, nickel and copper with 2-(4-methyl-2-quinolylazo)-5-diethylaminophenol by reversed-phase high-performance liquid chromatography.
Performance of six quinolylazo compounds was studied as a pre-column derivatizing reagent for the separation of metal ions in the reversed-phase high-performance liquid chromatography. 2-(4-Methyl-2-quinolylazo)-5-diethylaminophenol (QADP) was suited for the determination of Co, Ni and Cu in the presence of a large amount of Fe. The detection limits under the optimized conditions [stationary phase: L-column ODS; mobile phase: acetone-water (62:38, v/v) containing 0.01 M NH(4)SCN and 0.01 M 2-morpholinoethanesulfonic acid-NaOH (pH 6); injection volume: 10 mul] were 0.24 ng for Co, 0.22 for Ni and 0.53 for Cu. This method was successfully applied to the determination of these metal ions in a standard steel sample.
Niwa
H
H
Laboratory of Analytical Chemistry, Department of Applied Chemistry, Nagoya Institute of Technology, Showa, Nagoya 466, Japan.
Yasui
T
T
Ishizuki
T
T
Yuchi
A
A
Yamada
H
H
Wada
H
H