Semi-xylenol orange and its purification by high-pressure liquid chromatography.
By Mannich condensation of o-Cresol Red, iminodiacetic acid and formaldehyde, Semi-Xylenol Orange (SXO) has been prepared in a 10-hr batch-procedure with a yield of about 30%. From the crude product SXO has been isolated by reversed-phase HPLC with perchloric acid-acetone mixtures as the mobile phase and C(18)-bonded silica as the stationary phase. The SXO fraction was freed from accompanying perchloric acid by a second separation on the same column, with water as eluent. After elution with acetone, the SXO was crystallized by evaporation.
Smedes
F
F
Laboratory for Analytical Chemistry, University of Amsterdam, Nieuwe Achtergracht 166, Amsterdam, The Netherlands.
Decnop-Weever
L G
LG
Uyen
N T
NT
Nieman
J
J
Kragten
J
J