Microdetermination of alpha-amino acids by spectrophotometric and titrimetric methods.
Micro-methods for spectrophotometric and oxidimetric determination of alpha-amino-acids are described. They are based on a reaction of the acids with peri-naphthindan-2,3,4-trione hydrate at pH 2.5 to give a red precipitate of dihydroxy-peri-naphthindenone. The red product is dissolved and measured either spectrophotometrically at 243 nm or titrimetrically by reaction with iodine or N-bromosuccinimide. The results obtained by the three methods are concordant, the average recovery being 98 %.
Awad
W I
WI
Department of Chemistry, University College for Women, Ain Shams University, CairoU.A.R.