A new spectrophotometric method for the determination of primary aromatic amines.
A new spectrophotometric method for the assay of primary aromatic amines with metol-chromium(VI) reagent has been developed. The method is simple, reproducible and accurate within +/- 1.0%. It has been extended to the determination of drugs (in pure samples and pharmaceutical preparations) which either contain a free primary aromatic amine or release it through hydrolysis or reduction. The method is also applicable to the assay of p-aminobenzenesulphonamides in rat urine and blood. All the sulphonamide derivatives tested, as well as other aniline derivatives with an electron-withdrawing group in the para-position, afford nearly the same molar absorptivity. Depending on the absorptivity, 0.3-2 mumole of amine will give an absorbance of approximately 0.1.
Krishna
R R
RR
Department of Chemistry, Andhra University, Waltair-3, India.
Sastry
C S
CS