title: Elucidating the role of gonadal hormones in sexually dimorphic gene coexpression networks.
abstract: We previously used high-density expression arrays to interrogate a genetic cross between strains C3H/HeJ and C57BL/6J and observed thousands of differences in gene expression between sexes. We now report analyses of the molecular basis of these sex differences and of the effects of sex on gene expression networks. We analyzed liver gene expression of hormone-treated gonadectomized mice as well as XX male and XY female mice. Differences in gene expression resulted in large part from acute effects of gonadal hormones acting in adulthood, and the effects of sex chromosomes, apart from hormones, were modest. We also determined whether there are sex differences in the organization of gene expression networks in adipose, liver, skeletal muscle, and brain tissue. Although coexpression networks of highly correlated genes were largely conserved between sexes, some exhibited striking sex dependence. We observed strong body fat and lipid correlations with sex-specific modules in adipose and liver as well as a sexually dimorphic network enriched for genes affected by gonadal hormones. Finally, our analyses identified chromosomal loci regulating sexually dimorphic networks. This study indicates that gonadal hormones play a strong role in sex differences in gene expression. In addition, it results in the identification of sex-specific gene coexpression networks related to genetic and metabolic traits.
authors.affiliation: Department of Human Genetics, University of California, Los Angeles, California 90095-1679, USA.
authors.forename: Atila
authors.initials: A
authors.lastname: van Nas
authors.forename: Debraj
authors.initials: D
authors.lastname: Guhathakurta
authors.forename: Susanna S
authors.initials: SS
authors.lastname: Wang
authors.forename: Nadir
authors.initials: N
authors.lastname: Yehya
authors.forename: Steve
authors.initials: S
authors.lastname: Horvath
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Leslie
authors.initials: L
authors.lastname: Ingram-Drake
authors.forename: Gautam
authors.initials: G
authors.lastname: Chaudhuri
authors.forename: Eric E
authors.initials: EE
authors.lastname: Schadt
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Drake
authors.forename: Arthur P
authors.initials: AP
authors.lastname: Arnold
authors.forename: Aldons J
authors.initials: AJ
authors.lastname: Lusis
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics; D003433:Crosses, Genetic; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000187:drug effects / Q000502:physiology; D042341:Gonadal Hormones / Q000494:pharmacology / Q000502:physiology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012727:Sex Characteristics
chemical_list: D001057:Apolipoproteins E; D042341:Gonadal Hormones; D013196:Dihydrotestosterone; D004958:Estradiol