Nanometric layers for direct, signal-on, selective, and sensitive electrochemical detection of oligonucleotides hybridization.
We report a signal-on, reagentless electrochemical DNA biosensor, based on an electroactive self-assembled naphthoquinone derivative (JUG(thio)) monolayer. This system achieves highly sensitive (approximately 300 pM) and selective signal-on detection. Before hybridization, the single strand can interact with JUG(thio) and slow down the redox reaction. When the complementary target is added, the formation of the double helix eliminates the single strand/JUG(thio) interactions and the JUG(thio) redox rate, and hence the current increase.
March
Grégory
G
Interfaces, Traitement, Organisation et Dynamiques des Systèmes, Université Paris 7-Denis Diderot, UMR 7086, 15 rue Jean de Baif, 75205 Paris cedex 13, France.
Noël
Vincent
V
Piro
Benoît
B
Reisberg
Steeve
S
Pham
Minh-Chau
MC
D055664:Electrochemical Techniques / Q000379:methods*; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry*; D012680:Sensitivity and Specificity
D009841:Oligonucleotides