Identification of candidate genes for sporadic amyotrophic lateral sclerosis by array comparative genomic hybridization.
Amyotrophic lateral sclerosis (ALS) is a devastating disorder of the central nervous system that leads to progressive loss of upper and lower motor neurons. Most cases are sporadic and of unknown aetiology. In this study, we screened 72 patients with sporadic ALS for the presence of DNA copy number variations, in order to identify novel candidate disease genes. We have used sub-megabase resolution BAC array comparative genomic hybridization to detect genomic imbalances in our ALS patient cohort. Aberrations with potential relevance for disease aetiology were verified by oligo array CGH. In 72 patients with sporadic ALS, we identified a total of six duplications and five deletions that scored above our threshold. Nine of these 11 variations were smaller than 1Mb, and five were observed exclusively in ALS patients. In conclusion, non-polymorphic sub-microscopic duplications and deletions observable by array CGH are frequent in patients with sporadic ALS. Analysis of such aberrations serves as a starting point in deciphering the aetiology of this complex disease, given that affected genes can be considered candidates for influencing disease susceptibility.
Shoichet
Sarah A
SA
Max Planck Institute for Molecular Genetics, Berlin, Germany.
Waibel
Stefan
S
Endruhn
Sonja
S
Sperfeld
Anne D
AD
Vorwerk
Brita
B
Müller
Ines
I
Erdogan
Fikret
F
Ludolph
Albert C
AC
Ropers
Hans-Hilger
HH
Ullmann
Reinhard
R
D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics* / Q000503:physiopathology; D055028:Comparative Genomic Hybridization; D018628:Gene Dosage; D020022:Genetic Predisposition to Disease; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*