The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.
Biochemical assay of proteomic libraries derived from the Saccharomyces cerevisiae genome provides a powerful new tool for the assignment of activities to proteins. Particular advantages of this approach include the speed with which a protein can be identified and the generality for any biological activity for which an assay can be developed. We discuss the utility of this approach for the identification of RNA-modifying enzymes using a yeast proteomic library derived from a genomic set of strains expressing GST-ORF fusion proteins. This technique is also broadly applicable to other classes of RNA-protein interactions, including RNA binding and RNA degradation, and can be used with any of the proteomic libraries that are available.
Jackman
Jane E
JE
Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York, USA.
Grayhack
Elizabeth J
EJ
Phizicky
Eric M
EM
D011485:Protein Binding; D040901:Proteomics / Q000379:methods*; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D016601:RNA-Binding Proteins; D012313:RNA