Arylesterase and paraoxonase activity of paraoxonase (PON1) affected by ischemia in the plasma of patients with arterial occlusion of the lower limbs.
OBJECTIVE
The aim of the study was to evaluate the effect of different degrees of chronic ischemia of the lower limbs on PON1 activity in plasma, in relation to different substrates.


METHODS
The studied group consisted of patients with chronic arterial occlusion of the lower limbs due to atherosclerosis. The paraoxonase and arylesterase activities of PON1 were measured according to the Karen Gan method.


RESULTS
PON1 arylesterase activity was affected by ischemia of the lower limbs depending on its degree. In the group with critical ischemia decreased PON1 activity was observed in comparison with that in the moderate ischemia group and the control group (50.05+/-21.40 U/mL and 82.59+/-29.27 U/mL, 85.30+/-35.05 U/mL, respectively).


CONCLUSIONS
The results revealed a reverse relationship between PON1 activity and the progress of atherosclerosis to the ischemic level. The present study demonstrates, for the first time, that the arylesterase activity of PON1 is affected by critical ischemia of the lower limbs.
Kasprzak
Magdalena
M
Department of General Chemistry, Chemistry and Clinical Biochemistry, Poznań University of Medical Sciences, 6 Grunwaldzka Street, 60-780 Poznań, Poland. magdarut@ump.edu.pl
Iskra
Maria
M
Majewski
Wacław
W
Wielkoszyński
Tomasz
T
D000368:Aged; D001157:Arterial Occlusive Diseases / Q000097:blood*; D043303:Aryldialkylphosphatase / Q000097:blood / Q000378:metabolism*; D050197:Atherosclerosis / Q000097:blood; D002265:Carboxylic Ester Hydrolases / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007511:Ischemia / Q000097:blood* / Q000503:physiopathology*; D035002:Lower Extremity / Q000098:blood supply*; D008297:Male; D008875:Middle Aged
D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human