title: Fabrication of a highly sensitive penicillin sensor based on charge transfer techniques.
abstract: A highly sensitive penicillin biosensor based on a charge-transfer technique (CTTPS) has been fabricated and demonstrated in this paper. CTTPS comprised a charge accumulation technique for penicilloic acid and H(+) ions perception system. With the proposed CTTPS, it is possible to amplify the sensing signals without external amplifier by using the charge accumulation cycles. The fabricated CTTPS exhibits excellent performance for penicillin detection and exhibit a high-sensitivity (47.852 mV/mM), high signal-to-noise ratio (SNR), large span (1445 mV), wide linear range (0-25 mM), fast response time (<3s), and very good reproducibility. A very lower detection limit of about 0.01 mM was observed from the proposed sensor. Under optimum conditions, the proposed CTTPS outstripped the performance of the widely used ISFET penicillin sensor and exhibited almost eight times greater sensitivity as compared to ISFET (6.56 mV/mM). The sensor system is implemented for the measurement of the penicillin concentration in penicillin fermentation broth.
authors.affiliation: Department of Electrical and Electronic Engineering, Toyohashi University of Technology, 1-1, Hibari-gaoka, Tempaku-cho, Toyohashi 441-8580, Aichi, Japan.
authors.forename: Seung-Ro
authors.initials: SR
authors.lastname: Lee
authors.forename: M M
authors.initials: MM
authors.lastname: Rahman
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Sawada
authors.forename: Makoto
authors.initials: M
authors.lastname: Ishida
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D010088:Oxidoreductases / Q000737:chemistry*; D010406:Penicillins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055672:Static Electricity
chemical_list: D004800:Enzymes, Immobilized; D010406:Penicillins; D010088:Oxidoreductases