title: Molecular detection of Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' Infection in cats by direct PCR using whole blood without DNA extraction.
abstract: Detection of hemotropic Mycoplasma spp. infection was attempted in cats by PCR using whole blood without DNA extraction. A total 46 of 54 (85%) cats with suspected Mycoplasma spp. infection showed a positive reaction, corresponding completely with the results of standard PCR testing. The direct PCR assay was sensitive enough to detect more than 0.0061% parasitemia for ;C. M. haemominutum' and 0.0075% parasitemia for M. haemofelis. These data indicate that the direct PCR assay might be sufficient for use as a tool in clinical examinations.
authors.affiliation: Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, Sagamihara City, Kanagawa, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Watanabe
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hisasue
authors.forename: Takehisa
authors.initials: T
authors.lastname: Souma
authors.forename: Shuichi
authors.initials: S
authors.lastname: Ohshiro
authors.forename: Takatsugu
authors.initials: T
authors.lastname: Yamada
authors.forename: Ryo
authors.initials: R
authors.lastname: Tsuchiya
mesh_terms: D000740:Anemia / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D002371:Cat Diseases / Q000097:blood / Q000175:diagnosis / Q000382:microbiology*; D002415:Cats; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006400:Hematocrit / Q000662:veterinary; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial