title: [Comparison of oestradiol and testosterone levels in peripheral blood and spermatic cord blood in patients with secretory azoospermia].
abstract: OBJECTIVE
  Comparison of testosterone and oestradiol levels in spermatic cord blood and peripheral blood as a function of the results of testicular sperm extraction by surgical biopsy in a population of patients with nonobstructive azoospermia (NOA).
  
  
  METHODS
  Prospective, comparative, teaching hospital-sponsored, ethics committee-approved study of 30 patients with NOA. Plasma testosterone and oestradiol assays in peripheral blood and blood taken from the spermatic vein during testicular biopsy were performed simultaneously for each patient. Statistical analyses were performed with Mann-Whitney test and Spearman's correlation coefficient by univariate and multivariate analysis of variance (p<0.05).
  
  
  RESULTS
  No significant correlation was demonstrated between spermatic and peripheral blood levels of testosterone and oestradiol, regardless of the biopsy results. The spermatic oestradiol/testosterone ratio was significantly increased when testicular sperm extraction was negative (p=0.018).
  
  
  CONCLUSIONS
  The increase of the oestradiol/testosterone ratio in spermatic cord blood in the case of negative testicular sperm extraction suggests the hypothesis of greater conversion of testosterone to oestradiol in the testes. This could reflect increased aromatase activity in the absence of germ cells. Further studies using tissue markers of spermatogenesis should provide a better understanding of the physiological role of oestrogens in spermatogenesis and to refine the indications for testicular biopsy in patients with NOA.
authors.affiliation: Service d'urologie, hôpital Charles-Nicolle, CHU de Rouen, 1, rue de Germont, 76031 Rouen, France; Institut hospitalo-universitaire de recherche biomédicale, université de Rouen, Rouen, France.
authors.forename: G
authors.initials: G
authors.lastname: Pasquier
authors.forename: N
authors.initials: N
authors.lastname: Rives
authors.forename: A
authors.initials: A
authors.lastname: Bouzouita
authors.forename: R
authors.initials: R
authors.lastname: Caremel
authors.forename: L
authors.initials: L
authors.lastname: Sibert
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000097:blood*; D004958:Estradiol / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011446:Prospective Studies; D013085:Spermatic Cord; D013739:Testosterone / Q000097:blood*; D055815:Young Adult
chemical_list: D013739:Testosterone; D004958:Estradiol