Determination of urea in urine using a conductivity cell with surface acoustic wave resonator-based measurement circuit.
A conductivity cell employing a 61 MHz surface acoustic wave resonator-based measurement circuit was applied to the detection of the urea/urease reaction. The kinetic enzymatic parameters of the urease were estimated from the frequency shifts. The effects of pH, temperature and inhibitor on the response of the enzyme conductivity measurement system were investigated. The system was applied to rapid determination of urea in small urine samples. The lowest detection limit of urea was 30 ng/ml.
Chen
K
K
Department of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Liu
D
D
Nie
L
L
Yao
S
S