Double use of microwaves in fatty acid preparation for elaidic acid determination as phenacyl ester using high-performance liquid chromatography in Brazilian fat products.
Brazilian samples of fat products like margarines, butters and chocolates were prepared to reversed-phase high-performance liquid chromatography analysis, using a domestic microwave oven for fat hydrolysis and derivatization with phenacyl chloride. The total reaction time was 6 min, recoveries were above 94%. Total reproducibility for whole procedure were 3.5% R.S.D. for elaidic acid (n=3), the total chromatography analysis was 25 min.
Silva
Fabiano O
FO
Departamento de Química, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, Belo Horizonte-MG, Brazil. drhtcl@yahoo.com
Ferraz
Vany
V