title: Lincomycin, rational selection of high producing strain and improved fermentation by amino acids supplementation.
abstract: Based on the report that the introduction of the biosynthetic precursor of lincomycin, propylproline, could increase the production of lincomycin (Bruce et al. in US Patent 3,753,859, 1973), a mutant strain pro10-20, with resistance of feedback suppression of proline (an analog of propylproline) was thus selected and lincomycin production increased by 10%. The addition of three amino acids (L-proline, L-tyrosine, L-alanine) which are the precursors of propylproline to the fermentation medium was found to enhance the accumulation of L-dopa through different pathways and was favorable to lincomycin biosynthesis. The production of lincomycin was increased by 23, 10, 13%, respectively, with the addition of 0.05 g L(-1) L-proline at 60 h, 0.005 g L(-1) L-tyrosine and 0.1 g L(-1) L-alanine directly in the medium.
authors.affiliation: Department of Bioengineering, East China University of Science and Technology, Shanghai, China. rfye@ecust.edu.cn
authors.forename: Ruifang
authors.initials: R
authors.lastname: Ye
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000409:Alanine / Q000378:metabolism / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001698:Biomedical Engineering; D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation; D007980:Levodopa / Q000378:metabolism / Q000494:pharmacology; D008034:Lincomycin / Q000096:biosynthesis*; D009154:Mutation; D011392:Proline / Q000378:metabolism / Q000494:pharmacology; D011770:Pyruvate Kinase / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D014443:Tyrosine; D007980:Levodopa; D011392:Proline; D008034:Lincomycin; D011770:Pyruvate Kinase; D000409:Alanine