Optimization of reversed-phase separation of some nucleosides.
Optimization of the separation of ionogenic nucleosides in reversed-phase high-performance liquid chromatography can be based on the solvophobic theory of retention. The dependence of the capacity factors, selectivity, plate number and resolution on the pH may be calculated by knowing pK(a) and measuring the capacity factors for the compounds in the ionized and molecular forms. An increase in the eluent ionic strength (I) results in increased selectivity.
Galushko
S V
SV
Institute of Bioorganic Chemistry of the Ukrainian SSR Academy of Sciences, 252660 Kiev 94, U.S.S.R.