title: Acoustic microfluidic chip technology to facilitate automation of phage display selection.
abstract: Modern tools in proteomics require access to large arrays of specific binders for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solution to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. In the present study, we show how a microfluidic concept that utilizes particle separation in an acoustic force field can be used to efficiently separate antigen-bound from unbound members of such libraries in a continuous flow format. Such a technology has the hallmarks for incorporation in a fully automated selection system for the isolation of specific binders.
authors.affiliation: Department of Immunotechnology, Lund University, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Persson
authors.forename: Per
authors.initials: P
authors.lastname: Augustsson
authors.forename: Tomas
authors.initials: T
authors.lastname: Laurell
authors.forename: Mats
authors.initials: M
authors.lastname: Ohlin
mesh_terms: D000162:Acoustics; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D001331:Automation; D020811:Biomedical Technology; D008722:Methods; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D000936:Antigen-Antibody Complex; D019151:Peptide Library