title: Use of autologous bone marrow cells concentrate enriched with platelet-rich fibrin on corticocancellous bone allograft for posterolateral multilevel cervical fusion.
abstract: The outcomes of posterolateral multilevel spine fusion in difficult clinical settings, such as in an aged multi-diseased osteoporotic patient, remain unpredictable. The osteoprogenitor cells in bone marrow decrease with ageing without losing their osteogeneic potential. Autologous bone marrow cells (BMCs) from iliac crest aspirate can be concentrated in the operating room and platelet-rich fibrin (PRF) can be obtained from a peripheral blood as a source of autologous osteoprogenitor cells and growth factors, respectively. We present the case of an 88 year-old multi-diseased osteoporotic patient affected by cervical stenosis and subjected to C3--C7 posterior decompression, instrumentation and posterolateral fusion, using an intraoperative 'tissue-engineered' composite made of corticocancellous bone allograft augmented with autologous BMCs concentrate from iliac crest aspirate enriched with PRF from peripheral blood. Lateral dynamic X-rays and CT scan showed consolidation signs at 3 months follow-up, with solid C3--C7 fusion at 6 months follow-up. This paper describes a simple and effective method for potentially improving the fusion rate in aged osteoporotic patients by using corticocancellous bone allograft augmented with autologous BMCs concentrate from the iliac crest, enriched with PRF from peripheral blood, rapidly obtained before the surgical procedure.
authors.affiliation: Department of Orthopaedic and Trauma Surgery, Campus Bio-Medico University, Rome, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Vadalà
authors.forename: Alberto
authors.initials: A
authors.lastname: Di Martino
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Tirindelli
authors.forename: Luca
authors.initials: L
authors.lastname: Denaro
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Denaro
mesh_terms: D000369:Aged, 80 and over; D015703:Antigens, CD; D001792:Blood Platelets / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism*; D016025:Bone Transplantation; D002574:Cervical Vertebrae / Q000000981:diagnostic imaging / Q000473:pathology*; D005337:Fibrin / Q000378:metabolism*; D006801:Humans; D007430:Intraoperative Care; D008297:Male; D011859:Radiography; D013122:Spinal Diseases / Q000000981:diagnostic imaging / Q000628:therapy*; D013123:Spinal Fusion; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: D015703:Antigens, CD; D005337:Fibrin