Sequential injection spectrophotometric determination of phenylephrine hydrochloride in pharmaceutical preparations.
A fully automated sequential injection spectrophotometric method for the determination of phenylephrine hydrochloride in pharmaceutical preparations is reported. The method is based on the condensation reaction of the analyte with 4-aminoantipyrine in the presence of potassium ferricyanide. The absorbance of the condensation product was monitored at 503nm. A linear relationship between the relative peak height and concentration was obtained in the range 0.5-17.5mgl(-1). The detection limit (as 3sigma value) was 0.09mgl(-1) and repeatability was 0.8 and 0.6% at 2.5 and 5mgl(-1), respectively. Results obtained by this method agreed very well with those obtained by the AOAC official method.
Beyene
Negussie W
NW
Department of Chemistry, University of Pretoria, Pretoria 0002, South Africa.
Van Staden
Jacobus F
JF