Milk acidification by Lactococcus lactis is improved by decreasing the level of dissolved oxygen rather than decreasing redox potential in the milk prior to inoculation.
Although redox potential is very rarely taken into account in food fermentation it could be as influential as pH on bacterial activities. Lactococcus lactis is already known to exhibit a powerful reducing activity in milk but its reduction activity was shown to occur prior to its acidification activity with a potential interaction between these two lactococcal activities. Therefore, acidification lag-type phase could be shortened by decreasing the redox potential of milk before inoculation. As the redox potential is highly dependent on the dissolved oxygen level, our objective was to study their separate and combined influences on acidification and growth kinetics of pure L. lactis strains in milk. Results showed that high level of dissolved oxygen is significantly more influential on growth, and even more on acidification kinetics, than initial decreased redox potential of milk. Reduction of milk was drastic and mostly due to bacterial activity. The redox potential of milk only dropped when dissolved oxygen was entirely consumed. When there was no dissolved oxygen from the beginning, L. lactis immediately decreased the redox potential of milk and acidified afterwards. When the level of dissolved oxygen was initially high, acidification and reduction of milk occurred at the same time. Acidification kinetics was then biphasic with a slower rate during the aerobic stage and a faster rate during the anaerobic stage. The seven strains tested demonstrated diversity in both their acidification kinetics and their adaptation to high level of dissolved oxygen, independent of their growth kinetics. To conclude, we have shown that the level of dissolved oxygen in milk has a dramatic influence on acidification kinetics and could be used to control acidification kinetics in dairy industries.
Jeanson
Sophie
S
INRA, UR 342 Technologie et Analyses Laitières, Poligny, France. Sophie.Jeanson@rennes.inra.fr
Hilgert
Nadine
N
Coquillard
Marie-Odile
MO
Seukpanya
Céline
C
Faiveley
Marc
M
Neveu
Pascal
P
Abraham
Christophe
C
Georgescu
Véra
V
Fourcassié
Pascal
P
Beuvier
Eric
E
D000818:Animals; D005285:Fermentation / Q000502:physiology*; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013294:Lactococcus lactis / Q000254:growth & development / Q000378:metabolism*; D008892:Milk / Q000382:microbiology*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*
D010100:Oxygen