Multiple-timescale dynamics of side-chain carboxyl and carbonyl groups in proteins by 13C nuclear spin relaxation.
Side-chain carboxyl and carbonyl groups play a major role in protein interactions and enzyme catalysis. A series of (13)C relaxation experiments is introduced to study the dynamics of carboxyl and carbonyl groups in protein side chains on both fast (sub-ns) and slower (micros-ms) time scales. This approach is illustrated on the protein calbindin D(9k). Fast dynamics features correlate with hydrogen- and ion-binding patterns. We also identify chemical dynamics on micros time scales in solvent-exposed carboxyl groups, most probably due to exchange between the carboxylate and carboxylic acid forms.
Paquin
Raphaël
R
Département de Chimie, associé au CNRS, Ecole Normale Supérieure, 24, rue Lhomond, 75231 Paris Cedex 05, France.
Ferrage
Fabien
F
Mulder
Frans A A
FA
Akke
Mikael
M
Bodenhausen
Geoffrey
G
D002264:Carboxylic Acids / Q000737:chemistry*; D008954:Models, Biological; D019906:Nuclear Magnetic Resonance, Biomolecular; D050050:Protein Carbonylation; D011506:Proteins / Q000737:chemistry*
D002264:Carboxylic Acids; D011506:Proteins