A cytoplasmic protein-protein interaction detection method based on reporter translation.
One approach to drug discovery involves the targeting of abnormal protein-protein interactions that lead to pathology. We present a new technology allowing the detection of such interactions within the cytoplasm in a yeast-based system. The interaction detection is based on the sequestration of a translation termination factor involved in stop codon recognition. This sequestration inhibits the activity of the factor, thereby permitting the translation of a reporter gene harboring a premature stop codon. This novel cytoplasmic protein-protein interaction (CPPI) detection system should prove to be useful in the characterization of proteins as well as in partner identification, interaction mapping, and drug discovery applications.
Renaut
Laurence
L
MilleGen, Immeuble BIOSTEP, 31681 Labege Cedex, France. laurence.renaut@millegen.com
Bouayadi
Khalil
K
Kharrat
Hakim
H
Mondon
Philippe
P
D003593:Cytoplasm / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics; D008954:Models, Biological; D010443:Peptide Chain Termination, Translational / Q000235:genetics*; D025941:Protein Interaction Mapping / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism