title: Determination of Clobazam, Clorazepate, Flurazepam and Flunitrazepam in pharmaceutical preparations.
abstract: The separation of four benzodiazepines (Flurazepam, Flunitrazepam, Clobazam and Clorazepate) in pharmaceutical products by micellar electrokinetic chromatography (MEKC) is described. It was carried out at 25 degrees C and 25 kV by using a 57 cm (50 cm to the detector)x75 mum i.d. fused silica capillary and a 15 mM borate buffer (pH 9.2), 35 mM sodium dodecylsulfate (SDS) and 35 mM sodium deoxycholate water solution. Under these conditions, the analysis was performed in 8 min with acceptable limits of quantification (between 3 and 5 mg l(-1)). The method has been applied for quantifying these benzodiazepines in serum and different commercial formulations with recoveries near 100%.
authors.affiliation: Departamento de Quimica Analitica y Tecnologia de Alimentos, Facultad de Quimicas, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Berzas
authors.forename: G
authors.initials: G
authors.lastname: Castañeda
authors.forename: M J
authors.initials: MJ
authors.lastname: Pinilla