title: Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives.
abstract: BACKGROUND
  The Spemann/Mangold organizer is a transient tissue critical for patterning the gastrula stage vertebrate embryo and formation of the three germ layers. Despite its important role during development, there are still relatively few genes with specific expression in the organizer and its derivatives. Foxa2 is a forkhead transcription factor that is absolutely required for formation of the mammalian equivalent of the organizer, the node, the axial mesoderm and the definitive endoderm (DE). However, the targets of Foxa2 during embryogenesis, and the molecular impact of organizer loss on the gastrula embryo, have not been well defined.
  
  
  RESULTS
  To identify genes specific to the Spemann/Mangold organizer, we performed a microarray-based screen that compared wild-type and Foxa2 mutant embryos at late gastrulation stage (E7.5). We could detect genes that were consistently down-regulated in replicate pools of mutant embryos versus wild-type, and these included a number of known node and DE markers. We selected 314 genes without previously published data at E7.5 and screened for expression by whole mount in situ hybridization. We identified 10 novel expression patterns in the node and 5 in the definitive endoderm. We also found significant reduction of markers expressed in secondary tissues that require interaction with the organizer and its derivatives, such as cardiac mesoderm, vasculature, primitive streak, and anterior neuroectoderm.
  
  
  CONCLUSIONS
  The genes identified in this screen represent novel Spemann/Mangold organizer genes as well as potential Foxa2 targets. Further investigation will be needed to define these genes as novel developmental regulatory factors involved in organizer formation and function. We have placed these genes in a Foxa2-dependent genetic regulatory network and we hypothesize how Foxa2 may regulate a molecular program of Spemann/Mangold organizer development. We have also shown how early loss of the organizer and its inductive properties in an otherwise normal embryo, impacts on the molecular profile of surrounding tissues.
authors.affiliation: Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada. owen.tamplin@utoronto.ca
authors.forename: Owen J
authors.initials: OJ
authors.lastname: Tamplin
authors.forename: Doris
authors.initials: D
authors.lastname: Kinzel
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Cox
authors.forename: Christine E
authors.initials: CE
authors.lastname: Bell
authors.forename: Janet
authors.initials: J
authors.lastname: Rossant
authors.forename: Heiko
authors.initials: H
authors.lastname: Lickert
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian; D047108:Embryonic Development; D004627:Embryonic Induction; D005775:Gastrula; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D051542:Hepatocyte Nuclear Factor 3-beta / Q000235:genetics* / Q000502:physiology*; D017403:In Situ Hybridization; D051379:Mice; D046228:Microarray Analysis; D009154:Mutation; D020897:Organizers, Embryonic / Q000502:physiology*
chemical_list: C496503:Foxa2 protein, mouse; D051542:Hepatocyte Nuclear Factor 3-beta