Characterization of exopolysaccharide (EPS) produced by Weissella hellenica SKkimchi3 isolated from kimchi.
Weissella hellenica SKkimchi3 produces the higher exopolysaccharide (EPS) on sucrose than lactose, glucose, and fructose at pH 5 and 20 degrees C. Sucrose was exclusively used to cultivate SKkimchi3 in all experiments base on the EPS production tests. The molecular mass of EPS, as determined by gel permeation chroma-tography, was 203,000. (1)H and (13)C NMR analysis indicated that the identity of EPS may be a glucan. When EPS, starch, and cellulose was treated with a-amylase, glucoamylase, glucosidase, and cellulase, glucose was produced from starch and cellulose but was not produced from EPS. Based on HPLC analysis, elemental analysis, (1)H and (13)C NMR analysis, and enzymatic hydrolysis tests, EPS was estimated to be a glucan. EPS suspension was not precipitated even by centrifugation at 10,000xg for 60 min, and EPS made the fermented milk and bacterial culture viscous.
Kim
Min Ju
MJ
Department of Biological Engineering, Seokyeong University, Seoul 136-704, Republic of Korea.
Seo
Ha Na
HN
Hwang
Tae Sik
TS
Lee
Sung Hun
SH
Park
Doo Hyun
DH
D001937:Brassica / Q000378:metabolism / Q000382:microbiology*; D005285:Fermentation; D005516:Food Microbiology; D016937:Gram-Positive Endospore-Forming Rods / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D006868:Hydrolysis; D007723:Korea; D007785:Lactose / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism
D011135:Polysaccharides, Bacterial; D007785:Lactose