The use of polyethyleneimine for fabrication of potentiometric cholinesterase biosensors.
Potentiometric biosensors based on butyrylcholinesterase are developed by co-reticulation of the enzyme with glutaraldehyde on an electropolymerized polyethyleneimine film at the electrode surface. The BuChE-electrode was tested as biochemical sensor for detection of an organophosphorus pesticide, trichlorfon in liquid, the detection being based on the enzyme inhibition. The enzyme electrode showed a detection limit for trichlorfon below 10(-7) M.
Reybier
Karine
K
Laboratoire de Chimie des Matériaux et Interfaces, UFR Sciences and Techniques, 16 route de Gray-La Bouloie, 25030 Besancon Cedex, France.
Zairi
Sofiane
S
Jaffrezic-Renault
Nicole
N
Fahys
Bernard
B