title: Anti-inflammatory action of a phosphatidylcholine, phosphatidylethanolamine and N-acylphosphatidylethanolamine-enriched diet in carrageenan-induced pleurisy.
abstract: OBJECTIVE
  Phosphatidylcholine (PC)-derived choline exhibits anti-inflammatory properties in stress conditions. Phosphatidylethanolamine (PE) and N-acylphosphatidylethanolamines (NAPEs) are endogenous bioactive phospholipids linked to the PC and endocannabinoid metabolisms. We hypothesized that an increased dietary input of PC, PE and NAPE may interfere with leukocyte reactions and thus decreases the inflammatory activation.
  
  
  METHODS
  CFLP mice were fed with a control diet or with a diet supplemented with 1% PC, 0.4% PE and 0.1% NAPE for 7 days before the induction of pleurisy with carrageenan. Pleural leukocyte migration, pulmonary mast cell degranulation (Alcian blue-safranin O staining), and the activities of inducible nitric oxide synthase, xanthine oxidoreductase and myeloperoxidase were determined in lung tissue biopsies.
  
  
  RESULTS
  The carrageenan-induced inflammatory response was characterized by pulmonary leukocyte infiltration, mast cell degranulation and significantly increased inducible nitric oxide synthase and xanthine oxidoreductase activities (by 82 and 60%, respectively). Treatment of mice with acetylsalicylic acid or with dietary PC + PE + NAPE supplementation significantly decreased the leukocyte reaction, and suppressed the activity of the pulmonary proinflammatory enzymes.
  
  
  CONCLUSIONS
  This study confirms a potential for dietary PC + PE + NAPE supplementation to influence events crucial for the remission of acute inflammation. PC + PE + NAPE administration could possibly be a novel preventive or pharmacotherapeutic option in inflammatory pathologies.
authors.affiliation: Institute of Surgical Research, Faculty of Medicine, University of Szeged, Szeged, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Eros
authors.forename: G
authors.initials: G
authors.lastname: Varga
authors.forename: R
authors.initials: R
authors.lastname: Váradi
authors.forename: M
authors.initials: M
authors.lastname: Czóbel
authors.forename: J
authors.initials: J
authors.lastname: Kaszaki
authors.forename: M
authors.initials: M
authors.lastname: Ghyczy
authors.forename: M
authors.initials: M
authors.lastname: Boros
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D002351:Carrageenan / Q000633:toxicity; D015550:Cell Degranulation; D019587:Dietary Supplements; D007249:Inflammation / Q000178:diet therapy / Q000209:etiology / Q000473:pathology; D054709:Lecithins / Q000008:administration & dosage*; D007962:Leukocytes / Q000473:pathology; D008168:Lung / Q000201:enzymology / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000473:pathology / Q000502:physiology; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000008:administration & dosage*; D010998:Pleurisy / Q000178:diet therapy* / Q000209:etiology / Q000473:pathology; D014968:Xanthine Dehydrogenase / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D054709:Lecithins; D010714:Phosphatidylethanolamines; D002351:Carrageenan; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D014968:Xanthine Dehydrogenase