title: Trends in metal-binding and metalloprotein analysis.
abstract: This review describes recent tendencies for metal-binding and metalloprotein analysis, emphasizing metal quantification in proteins through X-ray, atomic absorption, mass spectrometric techniques, and others. Hyphenated techniques such as capillary electrophoresis-synchrotron radiation X-ray fluorescence (CE-SRXRF), laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), matrix-assisted laser desorption/ionisation-time-of-flight mass spectrometry (MALDI-TOF-MS), etc. are also presented. As protein separation techniques electrophoresis (mainly sodium dodecyl sulphate-polyacrylamide gel electrophoresis, SDS-PAGE), capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) are indicated, due to their inherent sensitivity, resolution and/or easy implementation. Latest challenges in metallomics are also commented.
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
authors.forename: Jerusa Simone
authors.initials: JS
authors.lastname: Garcia
authors.forename: Cristiana Schmidt de
authors.initials: CS
authors.lastname: Magalhães
authors.forename: Marco Aurélio Zezzi
authors.initials: MA
authors.lastname: Arruda