title: Phenylpropanoids from Daphne feddei and their inhibitory activities against NO production.
abstract: Chemical examination of the methanolic extract from the stem bark of Daphne feddei led to the isolation of five new phenylpropanoids, 4,4'-dihydroxy-3,3'-dimethoxy-9-butoxy-9,9'-epoxylignan (1), 4,4'-dihydroxy-3,3'-dimethoxy-9-ethoxy-9,9'-epoxylignan (2), daphneresinol (3), armaosigenin (4), and isocubebin (5), together with 33 known phenylpropanoids. All 38 compounds were isolated for the first time from D. feddei. All compounds were tested for inhibitory activity against LPS-induced NO production in RAW 264.7 macrophages. Compounds 2, 8, 9, 12, 13, and 15 showed potent inhibitory activities against the production of NO with IC(50) values of 0.091, 0.047, 0.005, 0.088, 0.004, and 0.074 μM/mL, respectively.
authors.affiliation: School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.forename: Yun-Heng
authors.initials: YH
authors.lastname: Shen
authors.forename: Jun-Mian
authors.initials: JM
authors.lastname: Tian
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Wu
authors.forename: Hui-Zi
authors.initials: HZ
authors.lastname: Jin
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
authors.forename: Shi-Kai
authors.initials: SK
authors.lastname: Yan
mesh_terms: D000818:Animals; D032364:Daphne / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D024301:Plant Bark / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D010666:Phenylpropionates; D009569:Nitric Oxide