Determination of thiol groups with o-diacetoxy-iodobenzoate.
o-Diacetoxyiodobenzoate is used for determining thiol groups by two procedures. In the first, thiols are titrated directly with the reagent at pH 6-8, with leuco-2,6-dichlorophenolindophenol and potassium iodide as indicator. In the second, thiols are treated at pH 7 with an excess of the reagent, the surplus being determined by reaction with excess of mercaptoacetic acid followed by back-titration of the latter with iodine. Both procedures yield results within 0.2% of the theoretical.
Srivastava
A
A
Department of Chemistry, University of Jabalpur, Jabalpur 482001, India.
Bose
S
S