Determination of superoxide dismutase in erythrocytes by a chemiluminescent assay.
A highly rapid chemiluminescent assay for the determination of superoxide dismutase (SOD) activity in erythrocytes was developed. The inhibition of the luminescent emission caused by the decrease of generated superoxide anions was measured. The aim of this work was to verify the application of a non amplified luminol SOD luminescent assay (CLM) in erythrocytes starting from an amplified method already used for the determination of XOD activity in milk (CLME). Both the assays had a detection limit of 3x10(-2)+/-7x10(-3) U/ml of SOD at 2sigma level, and a linear range of activity from 5.2 to 0.03 U/ml of SOD. The imprecision of assays (repeatability) presented coefficients of variations ranging from 3.1 to 7.9% for the CLME method and from 0.6 to 17.7% for CLM method. Both luminescent techniques were compared using a spectrophotometric kit, that had a detection limit of 0.3 U/ml, and showed good agreement.
Girotti
S
S
Istituto di Scienze Chimiche, Università di Bologna, Via S. Donato 15, 40127 Bologna, Italy.
Fini
F
F
Ferri
E
E
Budini
R
R
Piazzi
S
S
Cantagalli
D
D