title: Conformational changes in bovine lactoferrin induced by slow or fast temperature increases.
abstract: Lactoferrin (LF) is an iron-binding protein present in several secreted substances, such as milk, and has broad antimicrobial and physiological properties. Because high temperatures may affect protein stability and its functional properties, we investigated the effect of heat on bovine LF structure and stability. The effects of temperatures used during the pasteurization process on LF and its relationship to protein functionality were studied. Conformational changes were monitored using spectroscopic techniques, such as circular dichroism (CD) and fluorescence spectroscopy. The CD data at 70 degrees C showed that LF's secondary structure is drastically and irreversibly affected when the temperature is gradually increased. The same effect is observed when the temperature is gradually raised from 25 degrees C to 105 degrees C and changes are monitored by tryptophan fluorescence emission. We also verified the effects of simulating the pasteurization process; LF remained well structured during the entire process and this result was not time-dependent. Owing to preservation of the secondary structure with changes in the tertiary structure, we thus believe that pasteurization might cause LF to change into an intermediate partially folded state. A better understanding of heat stability is important for the use of LF as a bioactive component in food.
authors.affiliation: Mestrado em Ciência e Tecnologia do Leite, Universidade Norte do Paraná, 86041-100 Londrina, PR, Brazil.
authors.forename: Waleska D
authors.initials: WD
authors.lastname: Schwarcz
authors.forename: Lorena
authors.initials: L
authors.lastname: Carnelocce
authors.forename: Jerson L
authors.initials: JL
authors.lastname: Silva
authors.forename: Andréa C
authors.initials: AC
authors.lastname: Oliveira
authors.forename: Rafael B
authors.initials: RB
authors.lastname: Gonçalves
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D007781:Lactoferrin / Q000737:chemistry*; D008958:Models, Molecular; D011489:Protein Denaturation; D017434:Protein Structure, Tertiary; D013696:Temperature; D013997:Time Factors
chemical_list: D007781:Lactoferrin