title: Evaluation of two new immunochromatographic assays for diagnosis of malaria.
abstract: We assessed the performance of two new commercially available rapid diagnostic tests (RDTs) for malaria (SD Bioline Malaria Ag Pf test and Ag Pf/Pan test) in 200 patients with uncomplicated malaria between August and October 2007 in Madagascar. Results of the two RDTs were compared with those obtained by microscopy and real-time polymerase chain reaction. The sensitivity and specificity for detection of Plasmodium falciparum were 93% and 98.9%, respectively, for the SD Bioline Malaria Ag Pf test and 92.9% and 98.9% for the SD Bioline Malaria Ag Pf/Pan test. The sensitivity of the SD Bioline Malaria Ag Pf/Pan test was much lower for detection of other species (63.6%). The sensitivity of the two new assays decreased to 77.3% at parasitemia levels < 100 parasites/microL for detection of P. falciparum.
authors.affiliation: Malaria Research Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar.
authors.forename: Arsène
authors.initials: A
authors.lastname: Ratsimbasoa
authors.forename: Laza
authors.initials: L
authors.lastname: Fanazava
authors.forename: Rogelin
authors.initials: R
authors.lastname: Radrianjafy
authors.forename: Julien
authors.initials: J
authors.lastname: Ramilijaona
authors.forename: Hughes
authors.initials: H
authors.lastname: Rafanomezantsoa
authors.forename: Didier
authors.initials: D
authors.lastname: Ménard
mesh_terms: D000818:Animals; D002845:Chromatography / Q000379:methods*; D005260:Female; D006801:Humans; D008270:Madagascar; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D010963:Plasmodium falciparum / Q000302:isolation & purification; D012680:Sensitivity and Specificity