[Changes of RyR2 mRNA expression in rats suffering from acute myocardial ischemia].
OBJECTIVE
To investigate the changes of ryanodine receptor 2 (RyR2) mRNA expression in rats suffering from acute myocardial ischemia.


METHODS
SD rats were divided randomly into normal control group, myocardial ischemia group and sudden death group. The models of myocardial ischemia and sudden cardiac death were induced by intraperitoneal injection of hypophysine. The changes of RyR2 mRNA expression in cardiac sarcoplasmic reticulum (SR) of rats suffering from myocardial ischemia were detected by fluorescent RT-PCR technique.


RESULTS
The levels of RyR2 mRNA in the myocardial ischemia group and sudden death group were significant lower than those in the control group (P<0.05).


CONCLUSIONS
Myocardial ischemia may induce down-regulation of cardiac SR RyR2 mRNA expression.
Jie
Ya
Y
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhu
Shao-Hua
SH
Jiang
Yan-Wei
YW
Zhang
Lei
L
Fan
Fan
F
D000818:Animals; D016757:Death, Sudden, Cardiac; D015536:Down-Regulation; D005260:Female; D049429:Forensic Pathology; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel