Immunohistochemical analysis of lens cells on formation of different types of age-related cataract in humans.
The aim of the present work was to perform a comparative immunohistochemical study of the ocular lens in normal conditions and after formation of various type of age-related cataract in humans. The control group consisted of lenses from young men dying from accidents and serving as corneal donors (n = 10, not more than one hour after death). Lenses from patients (aged 60-70 years) undergoing surgery for cortical (n = 20) and nuclear (n = 20) cataracts were studied. The investigations demonstrated plasticity in the phenotype of lens cells in age-related cataract. Changes in the phenotype of lens cells were found to depend on the type of age-related cataract. Development of the cortical variant of age-related cataract was associated with positive reactions with monoclonal antibodies to neuron-specific enolase, S-100 protein, and vimentin only within the lens cortex. Formation of age-related nuclear cataract was associated with positive reactions with antibodies to alpha-smooth muscle actin and pancytokeratin, which were detected only in the lens nucleus. This provides evidence of the need to develop a differential approach to the treatment of different types of age-related cataract.
Korsakova
N V
NV
Department of Hospital Surgery, Chuvash State University, Cheboksary, Russia. korsnv@rambler.ru
Sergeeva
V E
VE
Petrov
S B
SB
D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D000375:Aging; D002386:Cataract / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D007904:Lens Cortex, Crystalline / Q000378:metabolism* / Q000473:pathology; D007907:Lens Nucleus, Crystalline / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D000199:Actins; D009418:S100 Proteins; D014746:Vimentin; D007633:Keratins; D010751:Phosphopyruvate Hydratase