[The study on growth and proliferation of neural stem cells from rats in vitro].
OBJECTIVE
To investigate culturing neural stem cells (NSCs) from rat embryos in vitro and to observe their growth and differentiation.


METHODS
NSCs were isolated from hippocampus of SD rat embryos (P16-P18) and cultured in DMEM/F12 medium containing EGF, bFGF, B27. To observe process of cell proliferation by microscope and identify cell types by immunocytochemical analyses after differentiation.


RESULTS
NSCs grew well in serum-free conditional medium and their cell bodies present transparent with good refraction at about eighth day. After differentiation, the cells demonstrated NSE and GFAP immunoreactive.


CONCLUSIONS
NSCs were cultured well in serum-free conditional medium and they could be induced to differentiate into neurons and astrocytes in serum conditional medium.
Chen
Qingguo
Q
Department of Otolaryngology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
Fu
Yong
Y
Wang
Xianhong
X
Wu
Xiaohui
X
Zhu
Rui
R
Li
Zewen
Z
Gong
Shusheng
S
D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004622:Embryo, Mammalian; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000196:embryology; D039902:Multipotent Stem Cells / Q000166:cytology; D058953:Neural Stem Cells / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D016895:Culture Media, Serum-Free