title: Crystal structure of mSULT1D1, a mouse catecholamine sulfotransferase.
abstract: In mammals, sulfonation as mediated by specific cytosolic sulfotransferases (SULTs) plays an important role in the homeostasis of dopamine and other catecholamines. To gain insight into the structural basis for dopamine recognition/binding, we determined the crystal structure of a mouse dopamine-sulfating SULT, mouse SULT1D1 (mSULT1D1). Data obtained indicated that mSULT1D1 comprises of a single alpha/beta domain with a five-stranded parallel beta-sheet. In contrast to the structure of the human SULT1A3 (hSULT1A3)-dopamine complex previously reported, molecular modeling and mutational analysis revealed that a water molecule plays a critical role in the recognition of the amine group of dopamine by mSULT1D1. These results imply differences in substrate binding between dopamine-sulfating SULTs from different species.
authors.affiliation: Laboratory of Structural Biology, Department of Systems Life Sciences, Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Teramoto
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: Kanako
authors.initials: K
authors.lastname: Inada
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Kurogi
authors.forename: Ming-Cheh
authors.initials: MC
authors.lastname: Liu
authors.forename: Masahito
authors.initials: M
authors.lastname: Suiko
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Kakuta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015239:Arylsulfotransferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004252:DNA Mutational Analysis; D004298:Dopamine / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary / Q000235:genetics; D015238:Sulfotransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: C431463:SULT1D1 sulfotransferase; D015238:Sulfotransferases; D015239:Arylsulfotransferase; D004298:Dopamine