title: Molecular clustering of STIM1 with Orai1/CRACM1 at the plasma membrane depends dynamically on depletion of Ca2+ stores and on electrostatic interactions.
abstract: Activation of store operated Ca(2+) entry involves stromal interaction molecule 1 (STIM1), localized to the endoplasmic reticulum (ER), and calcium channel subunit (Orai1/CRACM1), localized to the plasma membrane. Confocal microscopy shows that thapsigargin-mediated depletion of ER Ca(2+) stores in RBL mast cells causes a redistribution of STIM1, labeled with monomeric red fluorescent protein (mRFP), to micrometer-scale ER-plasma membrane junctions that contain Orai1/CRACM1, labeled with monomeric Aequorea coerulescens green fluorescent protein (AcGFP). Using fluorescence resonance energy transfer (FRET), we determine that this visualized coredistribution is accompanied by nanoscale interaction of STIM1-mRFP and AcGFP-Orai1/CRACM1. We find that antigen stimulation of immunoglobulin E receptors causes much less Orai1/CRACM1 and STIM1 association, but strong interaction is observed under conditions that prevent refilling of ER stores. Stimulated association monitored by FRET is inhibited by sphingosine derivatives in parallel with inhibition of Ca(2+) influx. Similar structural and functional effects are caused by mutation of acidic residues in the cytoplasmic segment of Orai1/CRACM1, suggesting a role for electrostatic interactions via these residues in the coupling of Orai1/CRACM1 to STIM1. Our results reveal dynamic molecular interactions between STIM1 and Orai1/CRACM1 that depend quantitatively on electrostatic interactions and on the extent of store depletion.
authors.affiliation: Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14850, USA.
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Calloway
authors.forename: Monika
authors.initials: M
authors.lastname: Vig
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Kinet
authors.forename: David
authors.initials: D
authors.lastname: Holowka
authors.forename: Barbara
authors.initials: B
authors.lastname: Baird
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D000071740:ORAI1 Protein; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013110:Sphingosine / Q000737:chemistry / Q000378:metabolism; D055672:Static Electricity; D000071737:Stromal Interaction Molecule 1; D019284:Thapsigargin / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D004791:Enzyme Inhibitors; D008562:Membrane Glycoproteins; D000071740:ORAI1 Protein; C532493:Orai1 protein, rat; D011993:Recombinant Fusion Proteins; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1; D019284:Thapsigargin; D013110:Sphingosine; D002118:Calcium