Staphylococcus aureus surface protein SasG contributes to intercellular autoaggregation of Staphylococcus aureus.
Staphylococcus aureus surface protein G (SasG) is one of cell surface proteins with cell-wall sorting motif. The sasG mutant showed significantly reduced cell aggregation and biofilm formation. SasG is comprised of variable A domain and multiple tandem repeats of B domain, native-PAGE and in vitro formaldehyde cross-linking experiments revealed that the recombinant protein of the A domain showed homo-oligomerization as an octamer, but B domain did not. This study shows that SasG-A domain contributes to intercellular autoaggregation by homo-oligomerization, and that may facilitate the adherence to host-tissues in the infection of S. aureus.
Kuroda
Makoto
M
Laboratory of Bacterial Genomics, Center for Pathogen Genomics, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. makokuro@nih.go.jp
Ito
Ryuta
R
Tanaka
Yoshikazu
Y
Yao
Min
M
Matoba
Kimio
K
Saito
Shinji
S
Tanaka
Isao
I
Ohta
Toshiko
T
D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D002462:Cell Membrane; D004252:DNA Mutational Analysis; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D017434:Protein Structure, Tertiary / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D008565:Membrane Proteins; C524374:SasG protein, Staphylococcus aureus