Surgical invasiveness of cell transplantation into the guinea pig cochlear modiolus.
OBJECTIVE
Previous studies have demonstrated the potential of cell transplantation for regeneration of spiral ganglion neurons (SGNs). However, the effect of surgical invasion on host cochleae has yet to be evaluated. The present study investigated the efficiency and invasiveness of our surgical procedure using a fine glass pipette for injections into the cochlear modiolus.


METHODS
We examined the survival of transplanted embryonic stem cell-derived neurons in the cochlear modiolus of guinea pigs. Surgical invasiveness was assessed by measurements of electrically evoked auditory brainstem responses (eABRs) and SGN densities after an injection of 5 microl of saline into the cochlear modiolus.


RESULTS
All of the transplanted animals exhibited localization of transplanted cells in the cochlear modiolus. No significant alterations in the eABR thresholds or SGN densities were found following surgery.


CONCLUSIONS
These findings indicate that our procedure is a viable method for testing the potential of transplants for SGN replacement.
Ogita
Hideaki
H
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Nakagawa
Takayuki
T
Lee
Kyu Yup
KY
Inaoka
Takatoshi
T
Okano
Takayuki
T
Kikkawa
Yayoi S
YS
Sakamoto
Tatsunori
T
Ito
Juichi
J
D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D002470:Cell Survival; D017690:Cell Transplantation / Q000379:methods*; D003051:Cochlea / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D004056:Differential Threshold; D053595:Embryonic Stem Cells / Q000473:pathology / Q000637:transplantation*; D016057:Evoked Potentials, Auditory, Brain Stem; D006168:Guinea Pigs; D007267:Injections; D019060:Minimally Invasive Surgical Procedures; D009474:Neurons / Q000473:pathology*; D011184:Postoperative Period; D012965:Sodium Chloride / Q000008:administration & dosage; D013136:Spiral Ganglion / Q000473:pathology; D016896:Treatment Outcome
D012965:Sodium Chloride