title: Inoculation of white-tailed deer (Odocoileus virginianus) with Ap-V1 Or NY-18 strains of Anaplasma phagocytophilum and microscopic demonstration of Ap-V1 In Ixodes scapularis adults that acquired infection from deer as nymphs.
abstract: Four white-tailed deer were inoculated with either the Ap-V1 or NY-18 strain of Anaplasma phagocytophilum. Ixodes scapularis nymphs were then allowed to acquistion feed on the inoculated deer and molt to adults. Only an Ap-V1 infected deer was infected persistently and able to infect nymphal Ixodes scapularis. Molted adult ticks maintained Ap-V1 infection as demonstrated by PCR and microscopy. We report, for the first time, a morphologic description of A. phagocytophilum in I. scapularis.
authors.affiliation: Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA. mason.reichard@okstate.edu
authors.forename: Mason V
authors.initials: MV
authors.lastname: Reichard
authors.forename: Raul Manzano
authors.initials: RM
authors.lastname: Roman
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Kocan
authors.forename: Edmour F
authors.initials: EF
authors.lastname: Blouin
authors.forename: José
authors.initials: J
authors.lastname: de la Fuente
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Snider
authors.forename: Rebecca E
authors.initials: RE
authors.lastname: Heinz
authors.forename: Misti D
authors.initials: MD
authors.lastname: West
authors.forename: Susan E
authors.initials: SE
authors.lastname: Little
authors.forename: Robert F
authors.initials: RF
authors.lastname: Massung
mesh_terms: D041081:Anaplasma phagocytophilum / Q000145:classification* / Q000502:physiology*; D000712:Anaplasmosis / Q000382:microbiology* / Q000635:transmission; D000818:Animals; D003670:Deer / Q000382:microbiology*; D018884:Ixodes / Q000382:microbiology*; D009758:Nymph / Q000382:microbiology