Fluorometric determination of nitrite.
A sensitive, simple and rapid fluorometric procedure for the determination of nitrite is described. It is based on the reaction of nitrite with 2,3-diaminonaphthalene in acidic solution to form 1-[H]-naphthotriazole, a highly fluorescent compound in alkaline medium. The detection limit is approximately 0.5 ng ml in the test sample (1% relative fluorescence intensity). Application of the method to analysis of a milk sample was tested with aliquots spiked with known amounts of nitrite.
Damiani
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P
Istituto di Chimica Bromatologica, Facoltà di Farmacia, Università degli Studi, 06100 Perugia, Italy.
Burini
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G