Evaluation of phenotypic and molecular methods for screening and detection of methicillin-resistant Staphylococcus aureus.
An in-house PCR was compared with the GenoType MRSA and the MRSA EVIGENE tests, both of which corresponded 100% with the results of in-house PCR. The cefoxitin disk diffusion method was superior to both the oxacillin disk diffusion and minimum inhibitory concentration tests for predicting the mecA status.
Kerttula
Anne-Marie
AM
Department of Bacteriology and Inflammatory Diseases, National Public Health Institute (KTL), Helsinki, Finland. anne-marie.kerttula@ktl.fi
Mero
Sointu
S
Pasanen
Tanja
T
Vuopio-Varkila
Jaana
J
Virolainen
Anni
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology
D000900:Anti-Bacterial Agents