In vivo metabolism of alpha-methyltryptamine in rats: identification of urinary metabolites.
1. The in vivo metabolism of alpha-methyltryptamine (AMT), a psychoactive tryptamine analogue, was studied in rats. 2. Male Wistar rats were administered 10 mg kg(-1) AMT orally and 24-h urine fractions were collected. After enzymatic hydrolysis of the urine sample, the metabolites were extracted by liquid-liquid extraction and analysed by gas chromatography/mass spectrometry (GC/MS). 3. 2-Oxo-AMT, 6-hydroxy-AMT, 7-hydroxy-AMT and 1'-hydroxy-AMT were detected as metabolites of AMT.
Kanamori
T
T
National Research Institute of Police Science, Kashiwa, Chiba, Japan. kanamori@nrips.go.jp
Kuwayama
K
K
Tsujikawa
K
K
Miyaguchi
H
H
Iwata
Y T
YT
Inoue
H
H
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014363:Tryptamines / Q000008:administration & dosage / Q000378:metabolism / Q000652:urine*
D014363:Tryptamines; C015293:indopan