[Study on aqueous chemical constituents from the flower buds of Eugenia caryophylla].
OBJECTIVE
To study the aqueous chemical constituents of the flower buds of Eugenia caryophylla.


METHODS
The compounds were isolated by HP-20 resin column chromatography, RP-C18 column chromatography, preparative RP-C18 thin-layer chromatography, preparative RP-C18 high performance liquid chromatography, and their structures were established by NMR and MS evidences.


RESULTS
Five compounds were separated from the water extracts. Their structures were identified as quercetin-3-O-glucuronide (1), quercetin-3-O-glucuronide 6"-methyl ester (2), quercetin-3-O-glucopy-ranoside (3) , eugenyl-beta-rutinoside (4) and myricetin (5).


CONCLUSIONS
Compounds 1-4 are isolated from the genus for the first time.
Liu
Hong-Yu
HY
Center for Health Food Evaluation, State Food and Drug Administration, Beijing 100070, China. liuhynn@hotmail.com
Zhu
Shu
S
Komatsu
Katsuko
K
Cai
Shao-Qing
SQ
Li
Ya-Hui
YH
Yu
Chao
C
Jiang
Yu
Y
Sa
Yi
Y
D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D035264:Flowers / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D027842:Syzygium / Q000737:chemistry*
D005419:Flavonoids; D005960:Glucosides; C525669:quercetin 3-O-(2''-galloyl)glucopyranoside; C443401:quercetin 3-O-glucuronide; C040015:myricetin; D011794:Quercetin