title: [The study on growth and proliferation of neural stem cells from rats in vitro].
abstract: OBJECTIVE
  To investigate culturing neural stem cells (NSCs) from rat embryos in vitro and to observe their growth and differentiation.
  
  
  METHODS
  NSCs were isolated from hippocampus of SD rat embryos (P16-P18) and cultured in DMEM/F12 medium containing EGF, bFGF, B27. To observe process of cell proliferation by microscope and identify cell types by immunocytochemical analyses after differentiation.
  
  
  RESULTS
  NSCs grew well in serum-free conditional medium and their cell bodies present transparent with good refraction at about eighth day. After differentiation, the cells demonstrated NSE and GFAP immunoreactive.
  
  
  CONCLUSIONS
  NSCs were cultured well in serum-free conditional medium and they could be induced to differentiate into neurons and astrocytes in serum conditional medium.
authors.affiliation: Department of Otolaryngology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Fu
authors.forename: Xianhong
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wu
authors.forename: Rui
authors.initials: R
authors.lastname: Zhu
authors.forename: Zewen
authors.initials: Z
authors.lastname: Li
authors.forename: Shusheng
authors.initials: S
authors.lastname: Gong
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004622:Embryo, Mammalian; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000196:embryology; D039902:Multipotent Stem Cells / Q000166:cytology; D058953:Neural Stem Cells / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016895:Culture Media, Serum-Free