title: Electropolymerized m-phenylenediamine as a means to immobilize active protein on thickness-shear-mode quartz crystal.
abstract: Electropolymerized m-phenylenediamine was used as an active coating for immobilizing urease and lectin on a gold-plated thickness-shear-mode (TSM) crystal. To enhance effectiveness of immobilization. a bilayer polymer film composed of polyaniline and poly-m-phenylenediamine was proposed. Compared with single poly-m-phenylenediamine film, the bilayer polymer film gave better results in terms of immobilizing capacity, stability and reproductivity. On this bilayer-film-coated TSM quartz crystal, the amount of immobilized lectin was estimated about 1.8 mug/cm(2). Detection of purified human erythrocytes is demonstrated as an example of potential application of this lectin-modified TSM biosensor in clinic.
authors.affiliation: New Material Research Institute, Hunan University, Changsha, 410082 P.R. China.
authors.forename: S H
authors.initials: SH
authors.lastname: Si
authors.forename: Y J
authors.initials: YJ
authors.lastname: Xu
authors.forename: L H
authors.initials: LH
authors.lastname: Nie
authors.forename: S Z
authors.initials: SZ
authors.lastname: Yao