PKMzeta inactivation induces spatial familiarity.
Spatial familiarization consists of a decrease in the exploratory activity over time after exposure to a place. Here, we show that a 30-min exposure to an open field led to a pronounced decrease in the exploratory behavior of rats, generating context familiarity. This behavioral output is associated with a selective decrease in hippocampal PKMzeta levels. A short 5-min exposure did not induce spatial familiarity or a decrease in PKMzeta, while inactivation of hippocampal PKMzeta by the specific inhibitor ZIP was sufficient to induce spatial familiarity, suggesting that the decrease in PKMzeta is involved in setting a given context as a familiar place.
Moncada
Diego
D
Facultad de Medicina, Instituto de Biología Celular y Neurociencias, Universidad de Buenos Aires, Buenos Aires, Argentina.
Viola
Haydée
H
D000704:Analysis of Variance; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005106:Exploratory Behavior / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D010455:Peptides / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D021641:Recognition, Psychology / Q000502:physiology*; D013037:Spatial Behavior / Q000502:physiology*; D013997:Time Factors
D004791:Enzyme Inhibitors; D010455:Peptides; D011493:Protein Kinase C; C504730:protein kinase M zeta, rat