Taxonomic revision of the nematode-trapping fungus Arthrobotrys multisecundaria.
The gene encoding an extracellular serine protease was cloned from Arthrobotrys multisecundaria using degenerate primers. The gene was highly similar (99.26%) to protease Mix from Monacrosporium microscaphoides. To clarify the taxonomic relationship between these species, genes encoding the internal transcribed spacer (ITS) and beta-tubulin were also cloned and sequenced from A. multisecundaria and M. microscaphoides, respectively. Homologous analysis of the nuclear (ITS) and protein (beta-tubulin) encoding genes showed that the two species of nematode-trapping fungi also shared extensive identity (99.82 and 99.63%, respectively), although they exhibited obvious differences in secondary conidia morphology. Accordingly, a taxonomic revision is recommended, with A. multisecundaria being revised as A. microscaphoides var. multisecundaria. In addition, the identified mutation may better facilitate the study of the sporulation of nematode-trapping fungi.
Li
Juan
J
Laboratory for Conservation and Utilization of Bio-resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, P R China.
Yang
Jinkui
J
Liang
Lianming
L
Zhang
Ke-Qin
KQ
D000595:Amino Acid Sequence; D000818:Animals; D001203:Ascomycota / Q000145:classification* / Q000166:cytology / Q000235:genetics*; D003001:Cloning, Molecular; D021901:DNA, Intergenic / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009348:Nematoda / Q000382:microbiology*; D010802:Phylogeny; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000235:genetics; D014404:Tubulin / Q000235:genetics
D021901:DNA, Intergenic; D005656:Fungal Proteins; D014404:Tubulin; D012697:Serine Endopeptidases