title: Fluorescent reaction between ascorbic acid and DAN and its analytical application.
abstract: Fluorescent reaction between ascorbic acid (AA) and 2,3-diamino-naphthalene (DAN) was studied. The experimental results showed that AA could react with DAN at pH = 10.2-10.5, and form the fluorescent heterocyclic condensation products which emitted strong fluorescence. The fluorescence intensity was measured in a 1 cm quartz cell with excitation and emission wavelengths of 400 and 520 nm, respectively. The relationship was obtained between the fluorescence intensity and AA concentration in the range of 2-300 mug ml(-1), the regression coefficient is 0.9993. The detection limit (signal-to-noise = 2) is 0.4 mug ml(-1).
authors.affiliation: Department of Chemistry, Shandong University, Jinan 250100, China.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: C
authors.initials: C
authors.lastname: Tong
authors.forename: N
authors.initials: N
authors.lastname: Jie
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Ren
authors.forename: J
authors.initials: J
authors.lastname: Hu