Changes of nitric oxide synthase activity and free methylarginines contents in regenerating rat liver after partial hepatectomy.
In the present study, liver regeneration rate (%) was increased up to 70% 3 days after partial hepatectomy (PH). Nitric oxide synthase (NOS) activity in liver tissue as well as serum nitrite/nitrate content had no timed response, revealing no significant difference between shamoperated and partially hepatectomized rat liver. Contents of free methylarginines in liver tissue were increased biphasically in a time-dependent manner after PH. However, those in serum did not exhibit the same patterns as in liver. Taken together, the results suggest that N(G)-monomethyl-L-arginine (MMA) and N(G), N(G)-dimethylarginine (DMA) play a role in inhibiting nitric oxide (NO) synthesis in regenerating rat liver because the increase of their contents was synchronized with NOS expression.
Lee
Y J
YJ
Department of Biochemistry, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea.
Nam
S W
SW
Seo
D W
DW
Ahn
S H
SH
Ko
Y K
YK
Sung
D S
DS
Han
J W
JW
Hong
S Y
SY
Lee
H W
HW