title: Plasma biochemical responses of the omnivorous crucian carp (Carassius auratus) to crude cyanobacterial extracts.
abstract: Healthy crucian carp (Carassius auratus) were treated by intraperitoneal (i.p.) injection of crude cyanobacterial extracts at two doses, 50 and 200 microg MC-LR equiv kg(-1) BW. High mortality (100%) was observed within 60 h post injection in the high-dose group. In the treated fish, activities of four plasma enzymes, alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH), all showed substantial increases, with both dose and time-dependent effects. These increases of enzyme activity indicate severe impairment occurred in the liver of crucian carp over time. Plasma concentrations of energy-related biomolecules including glucose (GLU), cholesterol (CHO), triglyceride (TG), and total protein (TP) showed marked changes in the high-dose group, possibly a nutritional imbalance correlated with the liver injury caused by intraperitoneal exposure to crude cyanobacterial extracts.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Fisheries College of Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Xuezhen
authors.initials: X
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.forename: Dapeng
authors.initials: D
authors.lastname: Li
authors.forename: Zechao
authors.initials: Z
authors.lastname: Shi
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D000458:Cyanobacteria / Q000737:chemistry*; D000087522:Cyanobacteria Toxins; D004798:Enzymes / Q000097:blood*; D006054:Goldfish / Q000378:metabolism / Q000502:physiology*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects*; D008387:Marine Toxins / Q000494:pharmacology*; D052998:Microcystins / Q000494:pharmacology*
chemical_list: D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D004798:Enzymes; D008387:Marine Toxins; D052998:Microcystins