Novel monoclonal antibodies recognizing different subsets of lymphocytes from the common marmoset (Callithrix jacchus).
Callithrix jacchus, the common marmoset, is a small new world primate that is considered effective as an experimental animal model for various human diseases. In this study, we generated monoclonal antibodies (mAbs) against common marmoset lymphocytes for immunological studies on the common marmoset. We established five hybridoma clones, 6C9, 10D7, 6F10, 7A4 and 5A1, producing anti-marmoset mAbs against cell surface antigens on marmoset T and/or B lymphocytes. We confirmed that 6C9 and 10D7 antibodies recognized CD45 antigen, and 6F10 antibody recognized CD8 antigen by flow cytometry using marmoset cDNA transfectants. We also tested them for application of immunoprecipitation, Western blot analysis and immunohistochemistry. We found that immunohistochemistry using marmoset spleen sections could be applied with all established mAbs but immunoprecipitation and the Western blot analysis could be applied with 6F10 and 10D7 antibodies but not with the other three mAbs. These results show that these monoclonal antibodies are useful for advancing immunological research on the common marmoset.
Ito
Ryoji
R
Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kawasaki 216-0001, Japan.
Maekawa
Shin-ichiro
S
Kawai
Kenji
K
Suemizu
Hiroshi
H
Suzuki
Shuzo
S
Ishii
Hajime
H
Tanioka
Yoshikuni
Y
Satake
Masanobu
M
Yagita
Hideo
H
Habu
Sonoko
S
Ito
Mamoru
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D016827:CD8 Antigens / Q000276:immunology*; D002144:Callithrix; D002469:Cell Separation; D003429:Cross Reactions; D000939:Epitopes; D005434:Flow Cytometry; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D008252:Macaca fascicularis; D018716:Molecular Mimicry; D011971:Receptors, Immunologic / Q000276:immunology; D012453:Saimiri; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D014162:Transfection
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D000939:Epitopes; D011971:Receptors, Immunologic; D017493:Leukocyte Common Antigens