Fast flow-injection fluorimetric determination of amiloride by using a solid sensing zone.
A rapid and simple flow-through solid phase spectrofluorimetric system is described in this paper for the determination of the diuretic amiloride in physiological fluid (serum) and pharmaceuticals. The sensor was developed in conjunction with a monochannel flow-injection analysis system with fluorimetric transduction. Amiloride was transitorily retained on cationic exchanger gel Sephadex SP-C25 placed in the detection area into the cell. The determination is carried out without any derivatization reaction, by measuring directly the intrinsic fluorescence of the analyte and using the peak height as analytical signal. The wavelengths of excitation and emission were 291 and 419 nm, respectively. Amiloride could be determined in the concentration ranges of 10-600 and 4-250 mugl(-1) at a sampling rate of 24 and 30 h(-1), respectively with detection limits of 0.92 and 0.33 mugl(-1) for 100, and 600 mul of sample volume injected, respectively. The relative standard deviations for ten independent determinations were better than 0.65%. The method was satisfactorily applied to the determination of amiloride in spiked biological fluids (serum) and pharmaceutical preparations without any pretreatment of the samples.
Domínguez-Vidal
A
A
Faculty of Experimental Sciences, Department of Physical and Analytical Chemistry, University of Jaén, 23071 Jaén, Spain.
Ortega-Barrales
P
P
Molina-Díaz
A
A