[Molecular characters of Centella asiatica found with RAPD technology].
OBJECTIVE
To analyze the DNA molecular characters of Centella asiatica with RAPD technology.


METHODS
With the genomic DNA as templates extracted from various source of Centella asiatica samples, optimized RAPD PCR reaction systems had been used. The random promers had been screened to amplify the specific molecular fragments of Centella asiatica.


RESULTS
The specific genetic bands of Centella asiatica species from various habitats were established which were highly stable and repeatable and obviously different from those of other families, genuses of plants such as Gynostemma pentaphylum, Tobacco, Cayratia japonica.


CONCLUSIONS
The developed method of RAPD analysis for the genetic character bands of Centella asiatica could be applied to identify real Centella asiatica from its spurious breed plants. The genetic character bands of Centella asiatica amplified with the RAPD method show high homogeneous in several samples from different habitats.
Ruan
Yuan
Y
Gung Medical University, Nanning 530021, China.
Mo
Rong-Hao
RH
Li
Min
M
Huang
Liu-Qing
LQ
Luo
Yu
Y
Li
Xiong-Ying
XY
Zhou
Juan
J
Wu
Yao-Sheng
YS
D028041:Centella / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D004340:Drug Contamination; D005819:Genetic Markers; D018745:Genome, Plant; D029774:Gynostemma / Q000145:classification / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers