title: Simultaneous spectrophotometric determination of diuretics by using multivariate calibration methods.
abstract: The wavelength range and number of factors used in partial least-squares (PLS) calibration for the resolution of the dihydralazine (DHZ)-hydrochlorothiazide (HCT) binary mixture and the dihydralazine-hydrochlorothiazide-reserpine ternary mixture were optimized in terms of the relative standard error (R.S.E.) and relative mean standard error (R.M.S.E.). Under the optimum conditions thus established, synthetic mixtures of the analytes can be resolved with errors and relative standard deviations (R.S.D) less than 4.5 and 1.0%, respectively. The ensuing method, which was validated by comparison with high performance liquid chromatography (HPLC), also gives good results with real samples (pharmaceutical preparations).
authors.affiliation: Departamento de Quimica Analitica, Nutrición y Bromatologia, Facultad de Quimica, Universidad de La Laguna, E-38204 La Laguna, Tenerife, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Luis
authors.forename: J M
authors.initials: JM
authors.lastname: Fraga
authors.forename: F
authors.initials: F
authors.lastname: Jiménez
authors.forename: A I
authors.initials: AI
authors.lastname: Jiménez
authors.forename: J J
authors.initials: JJ
authors.lastname: Arias