title: A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses.
abstract: BACKGROUND
  Three new human polyomaviruses have been recently described, and investigating their in vivo biology and pathogenicity will require sensitive and rational detection assays.
  
  
  OBJECTIVE
  To develop and evaluate a sensitive and rational assay for detection of the newly identified KI and WU polyomaviruses.
  
  
  METHODS
  A single-tube, dual-probe, real-time PCR assay for simultaneous detection and discrimination of KI and WU polyomaviruses was developed.
  
  
  RESULTS
  The assay had near single-molecule sensitivity for both viruses and no cross-reactivity was observed. A panel of 637 nasopharyngeal aspirates was screened, resulting in a frequency of 1.4% for KIPyV and 1.3% for WUPyV.
  
  
  CONCLUSIONS
  The dual-probe assay provides a rational approach for further studies of KIPyV and WUPyV.
authors.affiliation: Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Lindau
authors.forename: Annika
authors.initials: A
authors.lastname: Tiveljung-Lindell
authors.forename: Shan
authors.initials: S
authors.lastname: Goh
authors.forename: Torbjörn
authors.initials: T
authors.lastname: Ramqvist
authors.forename: Tobias
authors.initials: T
authors.lastname: Allander
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D009305:Nasopharynx / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011120:Polyomavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000821:virology; D055815:Young Adult