title: Novel fluorescence detection of free radicals generated in photolysis of fenvalerate.
abstract: Photoinduced decarboxylation via homolytic cleavage of the ester linkage generating two benzyl radicals being recoupled is known to be a major photolytic pathway of the insecticide fenvalerate in aqueous or organic solvents. A highly sensitive and selective fluorescence spectroscopic method was applied to detect these radicals generated under xenon lamp irradiation in organic solvents and aqueous acetonitrile solutions. The short-lived radicals were efficiently trapped by the nitroxide free radical having a primary amino group, and the resultant diamagnetic adducts were instantaneously derivatized with fluorescamine as a fluorescent probe. The highly fluorescent derivatives were successfully separated and detected by a reversed-phase high-performance liquid chromatography equipped with a fluorescence detector, and their structures were individually identified by liquid chromatography/mass spectrometry.
authors.affiliation: Environmental Health Science Laboratory, Sumitomo Chemical Co, Ltd, Takarazuka, Hyogo, Japan. suzukiy16@sc.sumitomo-chem.co.jp
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Katagi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005450:Fluorescamine; D005456:Fluorescent Dyes; D005609:Free Radicals / Q000032:analysis*; D009570:Nitriles / Q000737:chemistry* / Q000528:radiation effects; D010782:Photolysis; D011722:Pyrethrins / Q000737:chemistry* / Q000528:radiation effects; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D005609:Free Radicals; D009570:Nitriles; D011722:Pyrethrins; D005450:Fluorescamine; C017690:fenvalerate