Evaluation of antioxidative capability of the tomato (Solanum lycopersicum) skin constituents by capillary electrophoresis and high-performance liquid chromatography.
In the current study, phenolic compounds of the tomato (Solanum lycopersicum) skin extract were separated and their composition was determined by capillary electrophoresis and tandem high-performance liquid chromatography diode array detection-mass spectrometry (HPLC-DAD-MS/MS). Both the total phenolic and flavonoid contents of the extract were determined. The antioxidative capability of the extract was measured using a stable free radical 2,2-diphenyl-1-picrylhydrazyl assay. The monitoring of the radical scavenging capability of specific phenolic compounds was carried out both by capillary electrophoresis and HPLC-MS/MS.
Helmja
Kati
K
Department of Chemistry, Faculty of Science, Tallinn University of Technology, Tallinn, Estonia. helmja@staff.ttu.ee
Vaher
Merike
M
Püssa
Tõnu
T
Raudsepp
Piret
P
Kaljurand
Mihkel
M
D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary; D005419:Flavonoids / Q000032:analysis / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000378:metabolism; D010851:Picrates / Q000032:analysis / Q000378:metabolism; D019441:Plant Epidermis / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry
D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl