Effects of PEG-PLA-nano artificial cells containing hemoglobin on kidney function and renal histology in rats.
This study is to investigate the long-term effects of PEG-PLA nano artificial cells containing hemoglobin (NanoRBC) on renal function and renal histology after 1/3 blood volume top loading in rats. The experimental rats received one of the following infusions: NanoRBC in Ringer lactate, Ringer lactate, stroma-free hemoglobin (SFHB), polyhemoglobin (PolyHb), autologous rat whole blood (rat RBC). Blood samples were taken before infusions and on days 1, 7 and 21 after infusions for biochemistry analysis. Rats were sacrificed on day 21 after infusions and kidneys were excised for histology examination. Infusion of SFHB induced significant decrease in renal function damage evidenced by elevated serum urea, creatinine and uric acid throughout the 21 days. Kidney histology in SFHb infusion group revealed focal tubular necrosis and intraluminal cellular debris in the proximal tubules, whereas the glomeruli were not observed damaged. In all the other groups, NanoRBC, PolyHb, Ringer lactate and rat RBC, there were no abnormalities in renal biochemistry or histology. In conclusion, injection of NanoRBC did not have adverse effects on renal function nor renal histology.
Liu
Zun Chang
ZC
Artificial Cells & Organs Research Centre, Department of Physiology, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
Chang
Thomas M S
TM
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry; D001774:Blood Chemical Analysis; D001802:Blood Substitutes / Q000008:administration & dosage* / Q000378:metabolism; D002463:Cell Membrane Permeability; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology*; D006454:Hemoglobins / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D007262:Infusions, Intravenous; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007673:Kidney Cortex Necrosis / Q000473:pathology; D008297:Male; D053769:Nanocapsules / Q000008:administration & dosage*; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols; D051381:Rats; D017207:Rats, Sprague-Dawley
D001672:Biocompatible Materials; D001802:Blood Substitutes; D006454:Hemoglobins; D053769:Nanocapsules; D011091:Polyesters; C037158:polyhemoglobin; D011092:Polyethylene Glycols; C033616:poly(lactide)