title: Interaction of microglia and amyloid-beta through beta2-integrin is regulated by RhoA.
abstract: Amyloid-beta (Abeta) is one of the main factors to cause Alzheimer's disease. Although fibrillar Abeta (fAbeta) activates microglial cells that release toxic compounds to induce partial neuronal death, the mechanism of interaction between Abeta and microglia remains unclear. Therefore, we examined the interaction of microglial cells (BV2) and fAbeta on a gelatin-precoated plate. The binding was markedly enhanced by RhoA inactivation using Tat-C3, dominant negative RhoA, and si-RhoA. To identify the receptor for fAbeta, we tested various antibodies to mask receptors. Among them, anti-beta2-integrin antibody mostly suppressed cell binding to fAbeta. The incremental binding of cells induced by RhoA inhibition was also blocked by addition of anti-beta2-integrin antibody. These results suggest that RhoA inhibition stimulates beta2-integrin-mediated cell interaction to fAbeta.
authors.affiliation: Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Kangwon-Do, Republic of Korea.
authors.forename: Yee-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Won
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Moon
authors.forename: Won-Ho
authors.initials: WH
authors.lastname: Choi
authors.forename: Chun-Ho
authors.initials: CH
authors.lastname: Chang
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Jaebong
authors.initials: J
authors.lastname: Kim
authors.forename: Sung-Chan
authors.initials: SC
authors.lastname: Kim
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Jae-Bong
authors.initials: JB
authors.lastname: Park
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D018821:CD18 Antigens / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection / Q000379:methods; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D000906:Antibodies; D018821:CD18 Antigens; D010446:Peptide Fragments; D034741:RNA, Small Interfering; D020741:rho GTP-Binding Proteins