Production and characterization of the recombinant human mu-opioid receptor from transgenic silkworms.
The production of useful quantities of G protein-coupled receptors is a major problem not only for screening of various drug compounds but also in performing structural biology studies. To solve this problem, we investigated the possibility of using transgenic silkworms for the production of these receptors. Using the human mu-opioid receptor gene, we constructed three transgenic silkworm strains that produced mu-opioid receptors. The silkworms expressed significant amounts of the receptor in the fat body and silk gland. The product was evaluated using a saturation ligand-binding assay. The expressed receptor exhibited ligand affinity similar to that of an authentic sample, and the yield from the transgenic silkworm was comparable to that obtained using an Sf9-baculovirus expression system. As the mass rearing of transgenic silkworms has already been established, the silkworms can be adapted for production of large quantities of receptors.
Tateno
Mayuko
M
Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
Toyooka
Masaru
M
Shikano
Yohko
Y
Takeda
Shigeki
S
Kuwabara
Nobuo
N
Sezutsu
Hideki
H
Tamura
Toshiki
T
D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism; D016367:Baculoviridae / Q000378:metabolism; D012831:Bombyx / Q000235:genetics*; D006801:Humans; D008957:Models, Genetic; D017450:Receptors, Opioid, mu / Q000096:biosynthesis* / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
D017450:Receptors, Opioid, mu; D011994:Recombinant Proteins