title: Global protein stability profiling in mammalian cells.
abstract: The abundance of cellular proteins is determined largely by the rate of transcription and translation coupled with the stability of individual proteins. Although we know a great deal about global transcript abundance, little is known about global protein stability. We present a highly parallel multiplexing strategy to monitor protein turnover on a global scale by coupling flow cytometry with microarray technology to track the stability of individual proteins within a complex mixture. We demonstrated the feasibility of this approach by measuring the stability of approximately 8000 human proteins and identifying proteasome substrates. The technology provides a general platform for proteome-scale analysis of protein turnover under various physiological and disease conditions.
authors.affiliation: Department of Genetics, Center for Genetics and Genomics, Brigham and Women's Hospital, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Hsueh-Chi Sherry
authors.initials: HC
authors.lastname: Yen
authors.forename: Qikai
authors.initials: Q
authors.lastname: Xu
authors.forename: Danny M
authors.initials: DM
authors.lastname: Chou
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Zhao
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Elledge
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002453:Cell Cycle; D002460:Cell Line; D018076:DNA, Complementary; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000378:metabolism; D006207:Half-Life; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D014176:Protein Biosynthesis; D055550:Protein Stability; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014158:Transcription, Genetic; D000097573:Red Fluorescent Protein
chemical_list: D000596:Amino Acids; D018076:DNA, Complementary; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D046988:Proteasome Endopeptidase Complex; D011506:Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein; C485184:enhanced green fluorescent protein