title: Mass transfer effects on DNA hybridization in a flow-through microarray.
abstract: The goal of this study is to assess the influence of mass transfer phenomena on DNA hybridization kinetics in a flow-through, porous microarray for fast molecular testing. We present a scaled mathematical model of coupled convection, diffusion and reaction in porous media, which was used to simulate hybridization kinetics and to analyze the influence of convective transport on the reaction rate. In addition to computer simulations, we also present experimental data of hybridization collected on our microarray system for different flow rates. The results reported in this paper provide for a better understanding of the interaction between reaction and mass transfer processes during flow-through hybridization and suggest criteria for system design and optimization.
authors.affiliation: Philips Research, High Tech Campus, 5656 AE Eindhoven, The Netherlands. moccad@yahoo.com
authors.forename: Daniel
authors.initials: D
authors.lastname: Mocanu
authors.forename: Aleksey
authors.initials: A
authors.lastname: Kolesnychenko
authors.forename: Sonja
authors.initials: S
authors.lastname: Aarts
authors.forename: Amanda
authors.initials: A
authors.lastname: Troost-Dejong
authors.forename: Anke
authors.initials: A
authors.lastname: Pierik
authors.forename: Erik
authors.initials: E
authors.lastname: Vossenaar
authors.forename: Henk
authors.initials: H
authors.lastname: Stapert
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D004867:Equipment Design; D007700:Kinetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016062:Porosity; D013997:Time Factors