title: Aneuploidy affects proliferation and spontaneous immortalization in mammalian cells.
abstract: Aneuploidy, an incorrect number of chromosomes, is the leading cause of miscarriages and mental retardation in humans and is a hallmark of cancer. We examined the effects of aneuploidy on primary mouse cells by generating a series of cell lines that carry an extra copy of one of four mouse chromosomes. In all four trisomic lines, proliferation was impaired and metabolic properties were altered. Immortalization, the acquisition of the ability to proliferate indefinitely, was also affected by the presence of an additional copy of certain chromosomes. Our data indicate that aneuploidy decreases not only organismal but also cellular fitness and elicits traits that are shared between different aneuploid cells.
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.
authors.forename: Bret R
authors.initials: BR
authors.lastname: Williams
authors.forename: Vineet R
authors.initials: VR
authors.lastname: Prabhu
authors.forename: Karen E
authors.initials: KE
authors.lastname: Hunter
authors.forename: Christina M
authors.initials: CM
authors.lastname: Glazier
authors.forename: Charles A
authors.initials: CA
authors.lastname: Whittaker
authors.forename: David E
authors.initials: DE
authors.lastname: Housman
authors.forename: Angelika
authors.initials: A
authors.lastname: Amon
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation; D048429:Cell Size; D002471:Cell Transformation, Neoplastic; D016922:Cellular Senescence; D003470:Culture Media; D004622:Embryo, Mammalian; D005347:Fibroblasts; D018628:Gene Dosage; D015870:Gene Expression; D042822:Genomic Instability; D005947:Glucose / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D051379:Mice; D012692:Serial Passage; D014178:Translocation, Genetic; D014314:Trisomy
chemical_list: D003470:Culture Media; D005973:Glutamine; D005947:Glucose