title: The effects of media properties on the horseradish peroxidase-catalyzed fluorogenic reaction.
abstract: The effects of media properties including buffers, acidity, solvents and surfactant on horseradish peroxidase-catalyzed fluorogenic reaction were investigated. The results showed that the so-called non-fluorescent hydrogen donors were in fact fluorescent. There existed an acid-base equilibrium in the fluorescent dimer product. For p -hydroxyphenylpropionic acid, a pK(a) value of 8.0 for the product was obtained from its titration curve. The product fluorescence increased with higher pH, however, a longer time was needed to reach the reaction equilibrium due to the pH mismatch problem. Cationic micelles cetyltrimethylammonium bromide and cetyltrimethylammonium chloride could reduce the pH mismatch and offered a way to further increase the determination sensitivity. Finally, a micelle-enhanced flow-injection analysis of horseradish peroxidase is suggested.
authors.affiliation: Department of Chemistry, Xiamen University, Xiamen 361005, China.
authors.forename: Q G
authors.initials: QG
authors.lastname: Li
authors.forename: J G
authors.initials: JG
authors.lastname: Xu
authors.forename: X Z
authors.initials: XZ
authors.lastname: Huang
authors.forename: G Z
authors.initials: GZ
authors.lastname: Chen