title: Identification of differentially displayed proteins in cerebrospinal fluid of Parkinson's disease patients: a proteomic approach.
abstract: BACKGROUND
  Clinical proteomics has been widely used to identify differentially displayed proteins in blood and cerebrospinal fluid (CSF) to understand the molecular and cellular events leading to Parkinson's disease (PD). The close connection between CSF and the brain offers reliable and reproducible way to assess the majority of changes in the brain proteome profile directly into CSF throughout the course of neurodegeneration.
  
  
  METHODS
  We identified the differentially displayed proteins in CSF of PD patients as compared with controls using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and mass spectrometry.
  
  
  RESULTS
  Comparative 2-D PAGE electrophoretograms of CSF of PD patients with case controls and/or neurological controls revealed significant differential display of six protein spots. The differentially displayed proteins were identified as serum albumin precursor, serum albumin chain-A, hemoglobin beta fragment, mutant globin, proline rich repeat 14 (PRR 14) and serum transferrin N-terminal lobe. Although the level of hemoglobin beta fragment and mutant globin was attenuated, serum albumin precursor, serum albumin chain-A, PRR 14 and serum transferrin N-terminal lobe were augmented in PD patients as compared with case controls. The level of serum albumin chain-A, PRR 14 and serum transferrin N-terminal lobe was not significantly altered when compared with neurological controls.
  
  
  CONCLUSIONS
  The results obtained thus suggest that differential display of CSF serum albumin precursor, serum albumin chain-A, PRR 14 and serum transferrin N-terminal lobe could be associated with neuronal dysfunction and hemoglobin/globin with the onset/progression of PD in humans.
authors.affiliation: Indian Institute of Toxicology Research, Lucknow-226 001, India.
authors.forename: Ashima
authors.initials: A
authors.lastname: Sinha
authors.forename: Nalini
authors.initials: N
authors.lastname: Srivastava
authors.forename: Seema
authors.initials: S
authors.lastname: Singh
authors.forename: Anand Kumar
authors.initials: AK
authors.lastname: Singh
authors.forename: Shashi
authors.initials: S
authors.lastname: Bhushan
authors.forename: Rakesh
authors.initials: R
authors.lastname: Shukla
authors.forename: Mahendra Pratap
authors.initials: MP
authors.lastname: Singh
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010300:Parkinson Disease / Q000134:cerebrospinal fluid* / Q000473:pathology; D040901:Proteomics; D015203:Reproducibility of Results
chemical_list: D002556:Cerebrospinal Fluid Proteins