title: Chemical engineering of mesenchymal stem cells to induce a cell rolling response.
abstract: Covalently conjugated sialyl Lewis X (SLeX) on the mesenchymal stem cell (MSC) surface through a biotin-streptavidin bridge imparts leukocyte-like rolling characteristics without altering the cell phenotype and the multilineage differentiation potential. We demonstrate that the conjugation of SLeX on the MSC surface is stable, versatile, and induces a robust rolling response on P-selectin coated substrates. These results indicate the potential to increase the targeting efficiency of any cell type to specific tissue.
authors.affiliation: Department of Medicine, Harvard-MIT Division of Heath Sciences and Technology, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA.
authors.forename: Debanjan
authors.initials: D
authors.lastname: Sarkar
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Vemula
authors.forename: Grace S L
authors.initials: GS
authors.lastname: Teo
authors.forename: Dawn
authors.initials: D
authors.lastname: Spelke
authors.forename: Rohit
authors.initials: R
authors.lastname: Karnik
authors.forename: Le Y
authors.initials: le Y
authors.lastname: Wee
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Karp
mesh_terms: D001710:Biotin / Q000378:metabolism; D002454:Cell Differentiation; D002470:Cell Survival; D005453:Fluorescence; D006801:Humans; D036904:Leukocyte Rolling / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry; D019007:P-Selectin / Q000378:metabolism; D012680:Sensitivity and Specificity; D000080506:Sialyl Lewis X Antigen; D013194:Staining and Labeling; D019809:Streptavidin / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D019007:P-Selectin; D000080506:Sialyl Lewis X Antigen; D001710:Biotin; D019809:Streptavidin