Overexpression of adiponectin receptors potentiates the antiinflammatory action of subeffective dose of globular adiponectin in vascular endothelial cells.
OBJECTIVE
A decreased plasma level of adiponectin is associated with obesity and metabolic syndrome and correlated with endothelial dysfunction. This study aimed to investigate the regulated expression of the newly identified adiponectin receptors (AdipoR1 and 2) and their roles in the endothelial expression of intercellular adhesion molecule-1 (ICAM-1) in response to tumor necrosis factor (TNF)-alpha.


RESULTS
Immunohistochemical study and quantitative RT-PCR demonstrated that globular adiponectin suppressed the TNF-alpha-induced ICAM-1 expression in a dose-dependent manner in mouse aorta and human umbilical vein endothelial cells (HUVECs). Adenovirus-mediated overexpression of AdipoR1 and 2 in ECs significantly enhanced the suppressive effect of a subeffective dose of adiponectin on TNF-alpha-induced ICAM-1 expression and NF-kappaB activation. Promoter reporter assays and small interfering RNA revealed that peroxisome proliferator-activated receptor-alpha may function as an important pathway downstream of adiponectin and its receptors. Furthermore, overexpression of AdipoRs in rat carotid arteries markedly decreased the induction of ICAM-1 in vivo.


CONCLUSIONS
We provide novel evidence that upregulation of AdipoRs in ECs potentiates the antiinflammatory effect of adiponectin; modulating adiponectin receptors may have potential therapeutic applications for cardiovascular complications associated with metabolic syndrome and diabetes.
Zhang
Peng
P
Institute of Cardiovascular Sciences, Peking University Health Science Center, Beijing 100083, China.
Wang
Ying
Y
Fan
Yanbo
Y
Tang
Zhihui
Z
Wang
Nanping
N
D052242:Adiponectin / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000502:physiology; D002417:Cattle; D017931:DNA Primers; D003925:Diabetic Angiopathies / Q000517:prevention & control; D004730:Endothelium, Vascular / Q000502:physiology; D005786:Gene Expression Regulation; D020868:Gene Silencing; D017930:Genes, Reporter; D006801:Humans; D007249:Inflammation / Q000517:prevention & control*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D008156:Luciferases / Q000235:genetics; D051379:Mice; D016328:NF-kappa B / Q000502:physiology; D047493:PPAR alpha / Q000235:genetics; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics; D054419:Receptors, Adiponectin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology; D014471:Umbilical Veins / Q000502:physiology*
C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D052242:Adiponectin; D017931:DNA Primers; D016328:NF-kappa B; D047493:PPAR alpha; D034741:RNA, Small Interfering; D054419:Receptors, Adiponectin; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D008156:Luciferases