title: Determination of ciprofloxacin in human urine and serum samples by solid-phase spectrofluorimetry.
abstract: A method for the determination of trace amounts of ciprofloxacin has been developed, based on solid-phase spectrofluorimetry. The relative fluorescence intensity of ciprofloxacin fixed on Sephadex SP C-25 gel was measured directly after packing the gel beads in a 1-mm silica cell, using a solid-phase attachment. The wavelengths of excitation and emission were 272 and 448 nm, respectively. Using a sample volume of 1000 ml, the linear concentration range of application was 0.3-10.0 ng.ml(-1) of ciprofloxacin, with a R.S.D. of 1.2% (for a level of 4.0 ng.ml(-1)) and a detection limit of 0.1 ng.ml(-1). The method was applied to the determination of ciprofloxacin in human urine and serum samples. It was validated applying the standard addition methodology and using HPLC as a reference method. Recovery levels of the method reached 100% in all cases.
authors.affiliation: Research Group of Solid-Phase Spectrometry, Department of Analytical Chemistry, Faculty of Science, University of Granada, C/Fuentenueva, E-18071 Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Navalón
authors.forename: O
authors.initials: O
authors.lastname: Ballesteros
authors.forename: R
authors.initials: R
authors.lastname: Blanc
authors.forename: J L
authors.initials: JL
authors.lastname: Vílchez