title: A dynamic assessment of sperm DNA fragmentation versus sperm viability in proven fertile human donors.
abstract: OBJECTIVE
  To analyze any possible dynamic correlation between sperm DNA fragmentation and sperm viability.
  
  
  METHODS
  The rate of viability loss and the rate of increase of the frequency of sperm cells with fragmented DNA were determined at 0, 1.5, 4.5, and 24.0 hours after thawing samples from donors with proven fertility.
  
  
  METHODS
  Academic biology and reproductive medicine centers.
  
  
  METHODS
  Fifteen male donors with proven fertility for a maximum of six births at the reproductive medicine center.
  
  
  METHODS
  None.
  
  
  METHODS
  Sperm DNA fragmentation and viability dynamics expressed as logarithmic coefficients of change.
  
  
  RESULTS
  The dynamics of sperm DNA fragmentation and sperm viability adjusted to a logarithmic function with an initial highest velocity that progressively decreases. Nevertheless, the rates were not statistically significantly correlated.
  
  
  CONCLUSIONS
  In the short term, dynamic dysfunction of membrane permeability does not result in DNA fragmentation and thus must be considered as independent parameters of sperm quality.
authors.affiliation: Genetics Unit, Department of Biology, Universidad Autónoma de Madrid (UAM), Madrid, Spain. jaime.gosalvez@uam.es
authors.forename: Jaime
authors.initials: J
authors.lastname: Gosálvez
authors.forename: Elva I
authors.initials: EI
authors.lastname: Cortés-Gutiérrez
authors.forename: Rocio
authors.initials: R
authors.lastname: Nuñez
authors.forename: José Luís
authors.initials: JL
authors.lastname: Fernández
authors.forename: Pedro
authors.initials: P
authors.lastname: Caballero
authors.forename: Carmen
authors.initials: C
authors.lastname: López-Fernández
authors.forename: William V
authors.initials: WV
authors.lastname: Holt
mesh_terms: D002470:Cell Survival; D053938:DNA Fragmentation; D005260:Female; D005298:Fertility; D006801:Humans; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D027724:Reproductive Techniques, Assisted; D013074:Sperm Banks / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology; D014019:Tissue Donors