Quantitative analysis of three chiral pesticide enantiomers by high-performance column liquid chromatography.
Methods for the enantiomeric quantitative determination of 3 chiral pesticides, paclobutrazol, myclobutanil, and uniconazole, and their residues in soil and water are reported. An effective chiral high-performance liquid chromatographic (HPLC)-UV method using an amylose-tris(3,5-dimethylphenylcarbamate; AD) column was developed for resolving the enantiomers and quantitative determination. The enantiomers were identified by a circular dichroism detector. Validation involved complete resolution of each of the 2 enantiomers, plus determination of linearity, precision, and limit of detection (LOD). The pesticide enantiomers were isolated by solvent extraction from soil and C18 solid-phase extraction from water. The 2 enantiomers of the 3 pesticides could be completely separated on the AD column using n-hexane isopropanol mobile phase. The linearity and precision results indicated that the method was reliable for the quantitative analysis of the enantiomers. LODs were 0.025, 0.05, and 0.05 mg/kg for each enantiomer of paclobutrazol, myclobutanil, and uniconazole, respectively. Recovery and precision data showed that the pretreatment procedures were satisfactory for enantiomer extraction and cleanup. This method can be used for optical purity determination of technical material and analysis of environmental residues.
Wang
Peng
P
China Agricultural University, Department of Applied Chemistry, Beijing, People's Republic of China.
Liu
Donghui
D
Gu
Xu
X
Jiang
Shuren
S
Zhou
Zhiqiang
Z
D002851:Chromatography, High Pressure Liquid; D009570:Nitriles / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D010937:Plant Growth Regulators / Q000032:analysis*; D015203:Reproducibility of Results; D012987:Soil / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis; D013237:Stereoisomerism; D014230:Triazoles / Q000032:analysis*; D014867:Water / Q000032:analysis
D009570:Nitriles; D010573:Pesticide Residues; D010937:Plant Growth Regulators; D012987:Soil; D012989:Soil Pollutants; D014230:Triazoles; D014867:Water; C053370:paclobutrazol; C446685:systhane; C092705:uniconazole