High-performance liquid chromatographic method for the simultaneous determination of cyclosporine A and its four major metabolites in whole blood.
This study aimed to develop a simple and efficient optimized high-performance liquid chromatograph (HPLC) method for simultaneous determination of cyclosporine A (CyA) and its major, partly active metabolites AM1, AM9, AM4N, and AM19 in whole blood from transplant patients using cyclosporine D (CyD) as internal standard. The method used a CN analytical column maintained at 60 degrees C with hexan-isopropanol (93:7, v/v) as mobile phase; detection was at 212nm. Linearity for all five compounds was tested in the range of 31-1500ngml(-1) for CyA and of 31-1000ngml(-1) for metabolites. The limit of detection was found to be 15ngml(-1) for all compounds. This modified, inexpensive method is also suitable for measuring cyclosporine A and metabolite concentrations in routine monitoring of patients undergoing treatment with CyA.
Khoschsorur
G
G
Institute of Medical and Chemical Laboratory Diagnostics, University Medical Center, Auenbruggerplatz 29, Graz A-8036, Austria.
Erwa
W
W
Fruehwirth
F
F
Stettin
M
M
Meinitzer
A
A
Hoebarth
G
G
Zelzer
S
S
Halwachs-Baumann
G
G