Duddingtonia flagrans: centrifugal flotation technique with magnesium sulphate for the quantification and qualification of chlamydospores in sheep faeces.
Duddingtonia flagrans is a nematode-trapping fungus responsible for attacking larval stages of helminths in pasture, which has potential as a biological control method. The aim of this study was to test the magnesium sulphate centrifugal flotation technique for the quantification of D. flagrans chlamydospores in sheep faeces and to verify their morphological viability. In this experiment one sheep received an oral dose of 4.5 x 10(6) chlamydospores/day during 20 days. Fecal samples were collected between days 15 and 20 and analyzed by the centrifugal flotation technique with magnesium sulphate. Densities of 1.23, 1.27 and 1.31gmL(-1) recovered 1.45 x 10(5), 3.87 x 10(5) and 1.65 x 10(5) chlamydospores from the faeces, respectively. Based upon the results it was concluded that this is an efficient technique for the chlamydospores quantification in ovine faeces. Moreover, it allowed more accurate visualization of chlamydospore morphology.
Silva
Aleksandro Schafer da
AS
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil.
Zanette
Régis Adriel
RA
Otto
Mateus Anderson
MA
Soares
Cláudia Dal Molin
CD
Alves
Sydney Hartz
SH
Monteiro
Silvia Gonzalez
SG
Santurio
Janio Morais
JM
D000818:Animals; D001203:Ascomycota / Q000254:growth & development* / Q000302:isolation & purification; D002498:Centrifugation; D005243:Feces / Q000469:parasitology*; D005374:Filtration; D008278:Magnesium Sulfate; D008297:Male; D012756:Sheep; D013172:Spores, Fungal / Q000254:growth & development / Q000302:isolation & purification
D008278:Magnesium Sulfate