Determination of bromine in organic compounds by high-performance liquid chromatography.
A method is proposed for the determination of bromine in organic compounds (which may also contain chlorine and iodine) by oxygen-flask combustion of the compound followed by pre-column reaction of bromide with acetanilide and 2-iodosobenzoic acid to form 4-bromoacetanilide which is then chromatographed on an ODS column with a mobile phase of methanol: water, 65:35 v/v, detection at 240 nm, and 4-N-acetylaminotoluene as internal standard. The method is rapid and precise (RSD </= 1%), and applicable to a variety of bromine-containing organic compounds; the limit of detection is 0.2 ng of bromine.
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Department of Chemistry, Rani Durgavati University, Jabalpur 482001, Madhya Pradesh, India.
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