Electrochemical and spectroscopic evidences of the interaction between DNA and Pt(II)(dppf)-complex.
The interaction of Pt(II)(dppf)-complex, namely [Pt(dppf)(H(2)O)(2)](2+) with DNA was investigated by DPV and (1)H-NMR techniques. The results showed that the interaction process has been characterized by changes in the electrochemical parameters of both compounds and the formation of a new anodic current peak close to the anodic current peak of the [Pt(dppf)(H(2)O)(2)](2+). In addition, the (1)H-NMR spectra show that the coordination of Pt(II)(dppf)-complex to dsDNA occurs via N(7) of guanine. Others parameters like pH and ionic strength that affect the interaction process were also investigated.
Mello
Lucilene D
LD
Centro de Ciências Exatas e Tecnológicas, Universidade Federal do Pampa, Campus Bagé, Bage, RS, Brazil.
Ribeiro
Emerson S
ES
Kubota
Lauro T
LT
Elmroth
Sofi K C
SK
Pereira
Regina M S
RM
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002021:Buffers; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D019791:Guanidine / Q000737:chemistry; D006147:Guanine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008670:Metals / Q000737:chemistry; D008956:Models, Chemical; D010984:Platinum / Q000737:chemistry*; D011485:Protein Binding; D013053:Spectrophotometry / Q000379:methods*
D000970:Antineoplastic Agents; D002021:Buffers; D008670:Metals; D010984:Platinum; D006147:Guanine; D004247:DNA; D019791:Guanidine; D002945:Cisplatin