title: Intra-individual comparison of human ankle and knee chondrocytes in vitro: relevance for talar cartilage repair.
abstract: OBJECTIVE
  As compared to knee chondrocytes (KC), talar chondrocytes (TC) have superior synthetic activity and increased resistance to catabolic stimuli. We investigated whether these properties are maintained after TC are isolated and expanded in vitro.
  
  
  METHODS
  Human TC and KC from 10 cadavers were expanded in monolayer and then cultured in pellets for 3 and 14 days or in hyaluronan meshes (Hyaff-11) for 14 and 28 days. Resulting tissues were assessed biochemically, histologically, biomechanically and by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The proteoglycan and collagen synthesis rates in the pellets were also measured following exposure to Interleukin-1 beta (IL-1 beta).
  
  
  RESULTS
  After 14 days of pellet culture, TC and KC expressed similar levels of type I collagen (CI) and type II collagen (CII) mRNA and the resulting tissues contained comparable amounts of glycosaminoglycans (GAG) and displayed similar staining intensities for CII. Also proteoglycan and collagen synthesis were similar in TC and KC pellets, and dropped to a comparable extent in response to IL-1 beta. Following 14 days of culture in Hyaff-11, TC and KC generated tissues with similar amounts of GAG and CI and CII. After 28 days, KC deposited significantly larger fractions of GAG and CII than TC, although the trend was not reflected in the measured biomechanical properties.
  
  
  CONCLUSIONS
  After isolation from their original matrices and culture expansion, TC and KC displayed similar biosynthetic activities, even in the presence of catabolic stimuli. These in vitro data suggest a possible equivalence of TC and KC as autologous cell sources for the repair of talar cartilage lesions.
authors.affiliation: Department of Surgery, University Hospital, Basel, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Candrian
authors.forename: E
authors.initials: E
authors.lastname: Bonacina
authors.forename: J A
authors.initials: JA
authors.lastname: Frueh
authors.forename: D
authors.initials: D
authors.lastname: Vonwil
authors.forename: S
authors.initials: S
authors.lastname: Dickinson
authors.forename: D
authors.initials: D
authors.lastname: Wirz
authors.forename: M
authors.initials: M
authors.lastname: Heberer
authors.forename: M
authors.initials: M
authors.lastname: Jakob
authors.forename: I
authors.initials: I
authors.lastname: Martin
authors.forename: A
authors.initials: A
authors.lastname: Barbero
mesh_terms: D000328:Adult; D000368:Aged; D000843:Ankle Joint / Q000166:cytology* / Q000378:metabolism; D002358:Cartilage, Articular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D020219:Chondrogenesis / Q000502:physiology; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D007719:Knee Joint / Q000166:cytology* / Q000378:metabolism; D008875:Middle Aged; D011509:Proteoglycans / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013314:Stress, Mechanical
chemical_list: D006025:Glycosaminoglycans; D053583:Interleukin-1beta; D011509:Proteoglycans; D003094:Collagen