title: Immunohistochemical analysis of lens cells on formation of different types of age-related cataract in humans.
abstract: The aim of the present work was to perform a comparative immunohistochemical study of the ocular lens in normal conditions and after formation of various type of age-related cataract in humans. The control group consisted of lenses from young men dying from accidents and serving as corneal donors (n = 10, not more than one hour after death). Lenses from patients (aged 60-70 years) undergoing surgery for cortical (n = 20) and nuclear (n = 20) cataracts were studied. The investigations demonstrated plasticity in the phenotype of lens cells in age-related cataract. Changes in the phenotype of lens cells were found to depend on the type of age-related cataract. Development of the cortical variant of age-related cataract was associated with positive reactions with monoclonal antibodies to neuron-specific enolase, S-100 protein, and vimentin only within the lens cortex. Formation of age-related nuclear cataract was associated with positive reactions with antibodies to alpha-smooth muscle actin and pancytokeratin, which were detected only in the lens nucleus. This provides evidence of the need to develop a differential approach to the treatment of different types of age-related cataract.
authors.affiliation: Department of Hospital Surgery, Chuvash State University, Cheboksary, Russia. korsnv@rambler.ru
authors.forename: N V
authors.initials: NV
authors.lastname: Korsakova
authors.forename: V E
authors.initials: VE
authors.lastname: Sergeeva
authors.forename: S B
authors.initials: SB
authors.lastname: Petrov
mesh_terms: D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D000375:Aging; D002386:Cataract / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D007904:Lens Cortex, Crystalline / Q000378:metabolism* / Q000473:pathology; D007907:Lens Nucleus, Crystalline / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D000199:Actins; D009418:S100 Proteins; D014746:Vimentin; D007633:Keratins; D010751:Phosphopyruvate Hydratase