Non-random segregation of sister chromosomes in Escherichia coli.
It has long been known that the 5' to 3' polarity of DNA synthesis results in both a leading and lagging strand at all replication forks. Until now, however, there has been no evidence that leading or lagging strands are spatially organized in any way within a cell. Here we show that chromosome segregation in Escherichia coli is not random but is driven in a manner that results in the leading and lagging strands being addressed to particular cellular destinations. These destinations are consistent with the known patterns of chromosome segregation. Our work demonstrates a new level of organization relating to the replication and segregation of the E. coli chromosome.
White
Martin A
MA
Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
Eykelenboom
John K
JK
Lopez-Vernaza
Manuel A
MA
Wilson
Emily
E
Leach
David R F
DR
D002506:Cephalexin / Q000494:pharmacology; D020090:Chromosome Segregation; D002876:Chromosomes, Bacterial / Q000235:genetics* / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000166:cytology* / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000378:metabolism; D005092:Exonucleases / Q000378:metabolism; D008954:Models, Biological
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C489229:SbcC protein, E coli; D003851:Deoxyribonucleases; D005092:Exonucleases; C081229:sbcD protein, E coli; D002506:Cephalexin