Bisursodeoxycholate(ethylenediamine)platinum(II): a new autofluorescent compound. Cytotoxic activity and cell cycle analysis in ovarian and hematological cell lines.
The present paper describes for the first time an intrinsic fluorescent square-planar platinum(II) complex carrying two ursodeoxycholate ligands ([Pt(UDC)2(en)], where UDC(-) = ursodeoxycholate), that emits at room temperature once free in solution. Kinetic studies were carried out in aqueous solution and in the presence of different NaCl concentrations: 4 mM (similar to cytoplasmic concentration) and 150 mM (similar to plasmatic concentration). This novel compound was synthesized from a [PtCl2(en)] complex and shows increased cytotoxic activity against both resting and cycling HeLa cells, with no toxicity for cell lines derived from neoplastic haematopoietic cells.
Pérez-Andrés
Martín
M
Centro de Investigación del Cáncer (CIC) Service of Cytometry, Department of Medicine, Universidad de Salamanca, Salamanca, Spain.
Benito
Juan J
JJ
Rodríguez-Fernández
Emilio
E
Corradetti
Bruna
B
Primo
Daniel
D
Manzano
Juan L
JL
Orfao
Alberto
A
Criado
Julio J
JJ
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D019337:Hematologic Neoplasms / Q000378:metabolism*; D006801:Humans; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000378:metabolism*
D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D009944:Organoplatinum Compounds; C542727:bisursodeoxycholate(ethylenediamine)platinum(II)