title: A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of hydroxysafflor yellow A in human plasma: application to a pharmacokinetic study.
abstract: A sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the determination of hydroxysafflor yellow A (HSYA) in human plasma. HSYA was extracted from human plasma by using solid-phase extraction technique. Puerarin was used as the internal standard. A Shim-pack VP-ODS C(18) (150mm x 4.6mm, 5 microm) column and isocratic elution system composing of methanol and 5mM ammonium acetate (80:20, v/v) provided chromatographic separation of analytes followed by detection with mass spectrometry. The mass transition ion-pair was followed as m/z 611.19-->491.19 for HSYA and m/z 415.19-->295.10 for puerarin. The proposed method has been validated with a linear range of 1-1000 ng/ml for HSYA with a correlation coefficient >/=0.999. The lower limit of quantitation was 1 ng/ml. The intra-batch and inter-batch precision and accuracy were within 10%. The average extraction recovery was 81.7%. The total run time was 5.5 min. The validated method was successfully applied to the study on pharmacokinetics of HSYA in 12 healthy volunteers after a single oral administration of safflower oral solution containing 140 mg of HSYA.
authors.affiliation: Department of pharmacy, Xijing Hospital, Fourth Military Medical University, Shaanxi, China. adwen-2004@hotmail.com
authors.forename: AiDong
authors.initials: A
authors.lastname: Wen
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: YanYan
authors.initials: Y
authors.lastname: Jia
authors.forename: ZhiFu
authors.initials: Z
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Tian
authors.forename: Yin
authors.initials: Y
authors.lastname: Wu
authors.forename: ZhiRui
authors.initials: Z
authors.lastname: Wang
authors.forename: ZhongGui
authors.initials: Z
authors.lastname: He
mesh_terms: D002599:Chalcone / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D011809:Quinones / Q000097:blood* / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011809:Quinones; C085278:hydroxysafflor yellow A; D002599:Chalcone