title: Biomechanical analysis of combined treatment of high calcium and bisphosphonate in tibia of steroid-treated growing-phase rats.
abstract: Childhood systemic diseases are commonly treated with steroids. Consequently, steroid-induced osteoporosis is often observed as a side effect of steroid therapy. However, osteoporosis of tibia resulting from steroid therapy has not been reported yet. Herein we constructed a steroid-induced osteoporosis in tibia of the growing phase rats to examine internal structural changes of the bone and tried to find out the effect of bisphosphonates as a new and early treatment method. Biomechanical analysis was performed using two-dimensional microdensitometry and three-dimensional pQCT method. In addition, the following evaluations were carried out: noninvasive bone strength measurements in steroid-induced osteoporotic rat tibiae; comparing the effectiveness of single high-calcium diet versus combined treatment of high calcium and bisphosphonate for osteoporosis; and quantitative measurement of four elements (Ca, P, Mg, Zn) in bone matrix. Our data suggested that a combined treatment of high calcium and bisphosphonate was an effective new method to improve and treat steroid-induced osteoporosis in childhood.
authors.affiliation: Yokote Dental Clinic, 107-1 Koro, Koderacho, Himeji, Hyogo, 679-2151, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yokote
authors.forename: Emi
authors.initials: E
authors.lastname: Kimura
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Kimura
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kozono
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000627:therapeutic use*; D001846:Bone Development / Q000187:drug effects*; D001857:Bone Matrix / Q000737:chemistry; D002118:Calcium / Q000032:analysis; D002136:Calcium, Dietary / Q000627:therapeutic use*; D004195:Disease Models, Animal; D012968:Etidronic Acid / Q000627:therapeutic use*; D021621:Imaging, Three-Dimensional; D008274:Magnesium / Q000032:analysis; D008297:Male; D010024:Osteoporosis / Q000139:chemically induced* / Q000188:drug therapy; D010521:Periosteum / Q000187:drug effects / Q000473:pathology; D010758:Phosphorus / Q000032:analysis; D011241:Prednisone / Q000009:adverse effects / Q000031:analogs & derivatives*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D013977:Tibia / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D014057:Tomography, X-Ray Computed; D015032:Zinc / Q000032:analysis
chemical_list: D050071:Bone Density Conservation Agents; D002136:Calcium, Dietary; C046348:prednisone sodium succinate; D010758:Phosphorus; D008274:Magnesium; D015032:Zinc; D012968:Etidronic Acid; D002118:Calcium; D011241:Prednisone