title: Single peptide assembly onto a 1.5 nm Au surface via a histidine tag.
abstract: Nanoparticle surfaces functionalized with proteins or other biomolecules provide a mechanism for interfacing the unique properties of nanomaterials with biological samples. In most of these studies, the biomolecule is conjugated to a gold nanoparticles (AuNP) surface through the thiol group of native or introduced cysteine residues. Here we demonstrate the direct attachment of a hexa-histidine tagged (His(6)) peptide to a 1.5 nm AuNP. Binding occurs via a specific interaction between the Ne of the His imidazole, forming a 1:1 stoichiometric complex. Given the widespread use of histidine tags in producing recombinant proteins, this approach promises to expand the applications of AuNP in biological applications.
authors.affiliation: Department of Chemistry and Biochemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306, USA.
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Kogot
authors.forename: Hannah J
authors.initials: HJ
authors.lastname: England
authors.forename: Geoffrey F
authors.initials: GF
authors.lastname: Strouse
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Logan
mesh_terms: D004058:Diffusion; D006046:Gold / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010455:Peptides; D006639:Histidine; D006046:Gold