title: Cytoplasmic expression of human telomerase catalytic protein (hTERT) in neutrophils: an immunoelectron microscopy study.
abstract: Human telomerase comprises a catalytic protein subunit (hTERT) and an RNA subunit (hTR). Telomerase extends chromosome ends in compensation for the attrition of the telomeres during replication. In this work, the authors explore the expression of hTERT and hTR in neutrophils, respectively by immunochemistry techniques and in situ hybridization. hTERT was strongly expressed in neutrophils cytoplasm. The ultrastructural study showed that the gold particles were not associated with specific organelles but scattered in the cytosol. hTR was not expressed. hTERT is expressed in the cytoplasm of neutrophils, but its roles-eventually extratelomeric effects-remain to be elucidated.
authors.affiliation: Laboratoire d'Histologie-Embryologie, UFR II, Universite Victor Segalen Bordeaux, France. sebastien.lepreux@chu-bordeaux.fr
authors.forename: Sebastien
authors.initials: S
authors.lastname: Lepreux
authors.forename: Evelyne
authors.initials: E
authors.lastname: Doudnikoff
authors.forename: Incarnation
authors.initials: I
authors.lastname: Aubert
authors.forename: Paulette
authors.initials: P
authors.lastname: Bioulac-Sage
authors.forename: Bertrand
authors.initials: B
authors.lastname: Bloch
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Martin-Negrier
mesh_terms: D003593:Cytoplasm / Q000201:enzymology* / Q000648:ultrastructure; D006801:Humans; D017403:In Situ Hybridization; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D009504:Neutrophils / Q000201:enzymology* / Q000648:ultrastructure; D012313:RNA; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000032:analysis; D019098:Telomerase / Q000032:analysis*; D001743:Urinary Bladder / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; C422952:telomerase RNA; D012313:RNA; C509186:TERT protein, human; D019098:Telomerase