Influence of all-trans-retinoic acid on oxoglutarate carrier via retinoylation reaction.
All-trans-retinoic acid (atRA), an activated metabolite of vitamin A, is incorporated covalently into proteins both invivo and invitro. AtRA reduced the transport activity of the oxoglutarate carrier (OGC) isolated from testes mitochondria to 58% of control via retinoylation reaction. Labeling of testes mitochondrial proteins with (3)HatRA demonstrated the binding of atRA to a 31.5 KDa protein. This protein was identified as OGC due to the competition for the labeling reaction with 2-oxoglutarate, the specific OGC substrate. The role of retinoylated proteins is currently being explored and here we have the first evidence that retinoic acids bind directly to OGC and inhibit its activity in rat testes mitochondria via retinoylation reaction. This study indicates the evidence of a specific interaction between atRA and OGC and establishes a novel mechanism for atRA action, which could influence the physiological biosynthesis of testosterone in situations such as retinoic acid treatment.
Cione
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Department of Pharmaco-Biology, University of Calabria, Edificio Polifunzionale, 87036 Rende (CS), Italy.
Pingitore
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A
Perri
M
M
Genchi
G
G
D000818:Animals; D005033:Ethylmaleimide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008297:Male; D026901:Membrane Transport Proteins / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013737:Testis / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism / Q000494:pharmacology*
D026901:Membrane Transport Proteins; C021723:oxoglutarate translocator; D014212:Tretinoin; D005033:Ethylmaleimide