Determination of molybdenum, chromium and vanadium by ion-pair high-pressure liquid chromatography based on precolumn chelation with 4-(2-pyridylazo)resorcinol.
A reversed-phase ion-pair liquid chromatography-spectrophotometric detection system for the separation and simultaneous determination of molybdenum, chromium and vanadium is described. The chelates of the metal ions with 4-(2-pyridylazo)resorcinol are separated on a Zorbax CN column with 1 x 10(-3)M tetrabutylammonium iodide and 0.01M KH(2)PO(4)-Na(2)HPO(4) buffer (pH 7.50) in 30:70 v v methanol-water mixture as the mobile phase, at a flow-rate of 1.0 ml min . The chelates are detected spectrophotometrically at 540 nm.
Xiao-Song
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Department of Applied Chemistry, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.
Xiang-Ping
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Chang-Shan
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