Simultaneous determination of creatine and creatinine using amperometric biosensors.
In order to determine creatine and creatinine amperometric biosensors were proposed. A bienzymatic biosensor based on creatinase (CI) and sarcosine oxidase (SO) was used for the assay of creatine and a trienzymatic biosensor based on CI, SO and creatininase (CA) for the assay of creatinine. The linear concentration ranges are of pmol l(-1) to nmol l(-1) magnitude order, with very low limits of detection. The biosensors proved high reliability for determination of creatine and creatinine as raw material, and in the pharmaceutical formulation.
Stefan
Raluca-Ioana
RI
Department of Chemistry, University of Pretoria, Pretoria, South Africa. raluca.stefan@chem.up.ac.za
Bokretsion
Rahel Girmai
RG
van Staden
Jacobus F
JF
Aboul-Enein
Hassan Y
HY