Two-stage culture for producing berberine by cell suspension and shoot cultures of Berberis buxifolia Lam.
In vitro cultures of Berberis buxifolia were established using thidiazuron (4.5, 23 and 45 mM) or picloram (4 and 40 mM) as plant growth regulators for sustaining growth. For producing berberine, a two-stage culture was performed. In the first step, thidiazuron or picloram were used for biomass production followed by the production stage where benzylaminopurine (4.4 mM) was added as a plant growth regulator. Berberine yields (102 mg g(-1) DW) and in vitro shoot cultures (200 mg g(-1) DW) were significantly lower than those of whole plants in the field (416 mg g(-1) DW). The highest productivity (0.18 mg 1(-1) day(-1)) was attained using picloram (either 4 on 40 mM) in the first stage for producing biomass.
Alvarez
María A
MA
Instituto de Ciencia y Tecnología Dr César Milstein, CONICET, Saladillo, 2468 (C1440FFX), Ciudad de Buenos Aires, Argentina.
Eraso
Natalia Fernandez
NF
Pitta-Alvarez
Sandra I
SI
Marconi
Patricia L
PL
D001593:Benzyl Compounds / Q000494:pharmacology; D001599:Berberine / Q000302:isolation & purification* / Q000378:metabolism*; D028142:Berberis / Q000254:growth & development* / Q000378:metabolism*; D018929:Cell Culture Techniques; D010671:Phenylurea Compounds / Q000494:pharmacology; D010846:Picloram / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development* / Q000378:metabolism*; D011687:Purines / Q000494:pharmacology; D013830:Thiadiazoles / Q000494:pharmacology
D001593:Benzyl Compounds; D010671:Phenylurea Compounds; D010937:Plant Growth Regulators; D011687:Purines; D013830:Thiadiazoles; C016785:thidiazuron; D001599:Berberine; C480551:benzylaminopurine; D010846:Picloram