A rapid high-performance liquid chromatographic method for the determination of pantoprazole in plasma using UV detection. Application in pharmacokinetic studies.
A rapid, sensitive and reproducible HPLC method was developed and validated for the analysis of pantoprazole (CAS 102625-70-7) in human plasma. The separation was achieved on a monolithic silica column using acetonitrile-potassium dihydrogen phosphate buffer (25:75,v/v), pH 3.0, as the mobile phase at a flow rate of 1.5 ml min(-1). The wavelength was set at 290 nm. The assay enables the measurement of pantoprazole for therapeutic drug monitoring with a minimum quantification limit of 20 ng ml(-1). The method involves a simple protein precipitation procedure. Analyticil recovery was complete. The calibration curve was linear over the concentration range 20-3500 ng ml(-1) The coefficients of variation forthe inter-day and intra-day assay were found to be less than 7%.
Zarghi
Afshin
A
Department of Pharmaceutical Chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran. azarghi@yahoo.com
Shafaati
Alireza
A
Foroutan
Seyed Mohsen
SM
Movahed
Hooman
H
Khoddam
Arash
A
D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000097:blood* / Q000493:pharmacokinetics*; D000328:Adult; D000897:Anti-Ulcer Agents / Q000097:blood* / Q000493:pharmacokinetics*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D000077402:Pantoprazole; D010726:Phosphodiesterase Inhibitors / Q000097:blood / Q000493:pharmacokinetics; D010879:Piperazines / Q000097:blood / Q000493:pharmacokinetics; D011687:Purines / Q000097:blood / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D000068677:Sildenafil Citrate; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013450:Sulfones / Q000097:blood / Q000493:pharmacokinetics
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000897:Anti-Ulcer Agents; D007202:Indicators and Reagents; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D012996:Solutions; D013450:Sulfones; D000068677:Sildenafil Citrate; D000077402:Pantoprazole