Phosphorylation of CAP-G is required for its chromosomal DNA localization during mitosis.
Condensin is a 5 subunit complex that plays an important role in the structure of chromosomes during mitosis. It is known that phosphorylation of condensin subunits by cdc2/cyclin B at the beginning of mitosis is important for condensin activity, but the sites of these phosphorylation events have not been identified nor has their role in regulating condensin function. Here we identify two threonine residues in the CAP-G subunit of condensin, threonines 308 and 332, that are targets of cdc2/cyclin B phosphorylation. Mutation of these threonines to alanines results in defects in CAP-G localization with chromosomes during mitosis. These results are the first to identify phosphorylation sites within the condensin complex that regulate condensin localization with chromosomal DNA.
Murphy
Lynea A
LA
Department of Toxicology, Chandler Medical Center, University of Kentucky, Lexington, KY 40536, USA.
Sarge
Kevin D
KD
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000409:Alanine / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002877:Chromosomes, Human / Q000235:genetics / Q000378:metabolism*; D019926:Cyclin B / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010766:Phosphorylation; D013912:Threonine / Q000235:genetics / Q000378:metabolism; D014981:Xenopus
D018797:Cell Cycle Proteins; D019926:Cyclin B; D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; C533611:NCAPG protein, human; C108312:condensin complexes; D013912:Threonine; D004247:DNA; D016203:CDC2 Protein Kinase; D000251:Adenosine Triphosphatases; D000409:Alanine