Human osteoblasts adhesion and proliferation on magnesium-substituted tricalcium phosphate dense tablets.
Tricalcium phosphate (TCP) is recognized as a promising bone replacement material due to its high bioactivity and resorbable properties. To mimic biological apatites, incorporation of magnesium (Mg) in TCP was proposed. Mg-substituted TCP (beta-TCMP) and beta-TCP dense tablets were obtained by pressing and sintering at 1,000 degrees C Mg-substituted calcium deficient apatite (Mg-CDA) and commercial TCP, respectively. The materials were characterized using X-ray diffraction, infrared spectroscopy and electron microscopy. Human osteoblast cells (SaOs2) were seeded onto the sintered tablets for 4 h, 24 h and 7 days. Results showed that Mg-CDA was completely transformed into beta-TCMP. Moreover, beta-TCMP stimulated adhesion and proliferation of human osteoblast cells. Consequently, the magnesium incorporation on calcium deficient apatites followed by sintering at 1,000 degrees C seems to be a useful path to obtain biocompatible and non cytotoxic dense tablets with TCP structure with potential application on bone engineering.
Sader
Marcia S
MS
Metallurgical and Materials Department, COPPE/UFRJ, Rio de Janeiro, RJ, Brazil.
Legeros
Racquel Z
RZ
Soares
Gloria A
GA
D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D008274:Magnesium / Q000737:chemistry*; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D013607:Tablets / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
D018786:Bone Substitutes; D002130:Calcium Phosphates; D013607:Tablets; D008274:Magnesium; C018392:tricalcium phosphate