Selective extraction of bacterial DNA from the surfaces of macroalgae.
A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing.
Burke
Catherine
C
School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, The University of New South Wales, Sydney 2052, Australia.
Kjelleberg
Staffan
S
Thomas
Torsten
T
D004269:DNA, Bacterial / Q000302:isolation & purification*; D008967:Molecular Biology / Q000379:methods*; D000461:Rhodophyta / Q000382:microbiology*; D012680:Sensitivity and Specificity; D044445:Ulva / Q000382:microbiology*
D004269:DNA, Bacterial