Enhanced regeneration of critical bone defects using a biodegradable gelatin sponge and beta-tricalcium phosphate with bone morphogenetic protein-2.
We examine the osteogenicity of a sponge biomaterial consisting of a biodegradable mixture of gelatin and beta-tricalcium phosphate (betaTCP) that bound bone morphogenetic protein 2 (BMP-2) in critical-sized bone defects in rats. Gelatin-betaTCP sponges containing either phosphate buffered saline or incorporating BMP-2 are implanted into 5 mm diameter bone defects created in rat mandibles. We assess the defects biweekly for 8 weeks following implantation. There is significantly higher osteoinductive activity and significantly more Gla-osteocalcin content at bone-defect healing sites treated with gelatin-betaTCP sponges incorporating BMP-2 than there is in those treated with sponges that did not contain BMP-2. Histologically, new bone that contains bone marrow and that is connected to the original bone almost entirely replaces the regenerated bone. These results show that biodegradable gelatin-betaTCP incorporating BMP-2 is osteogenic enough to promote healing in large bone defects.
Matsumoto
Goichi
G
Department of Oral and Maxillofacial Surgery, Kanagawa Dental College 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan. oslab@kdcnet.ac.jp.
Omi
Yasushi
Y
Kubota
Eiro
E
Ozono
Satoru
S
Tsuzuki
Hideko
H
Kinoshita
Yukihiko
Y
Yamamoto
Masaya
M
Tabata
Yasuhiko
Y
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D001861:Bone Regeneration; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000627:therapeutic use*; D005780:Gelatin / Q000627:therapeutic use*; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009457:Neuroglia / Q000378:metabolism; D015675:Osteocalcin / Q000737:chemistry; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D013552:Swine
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D015675:Osteocalcin; D005780:Gelatin