title: Amperometric glucose enzyme electrode by immobilizing glucose oxidase in multilayers on self-assembled monolayers surface.
abstract: A novel and robust amperometric enzyme electrode for the determination of glucose was constructed by immobilizing glucose oxidase (GOD) and Os(bpy)(2)Cl-poly(4-vinyl)pyridine (Os-PVP) complex multilayers on thiol self-assembled monolayers surface. The apparent Michaelis-Menton constant K(m)' increased with increasing the number of Os-PVP/GOD multilayers. The concentration range of linear response and detection limit were 0.1-10 and 0.05 mM, the interference of ascorbic acid and uric acid were eliminated by the presence of SAMs and the enzyme electrodes were stable over 3 weeks. The preparation technique may be useful for controlling the performance of multilayer enzyme electrodes by changing the enzyme content.
authors.affiliation: Department of Chemistry, the State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, PR China.
authors.forename: S F
authors.initials: SF
authors.lastname: Hou
authors.forename: K S
authors.initials: KS
authors.lastname: Yang
authors.forename: H Q
authors.initials: HQ
authors.lastname: Fang
authors.forename: H Y
authors.initials: HY
authors.lastname: Chen