[Determination of endogenous anabolic steroids in hair using gas chromatography-tandem mass spectrometry].
A method of gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the simultaneous identification and quantification of five endogenous anabolic steroids (testosterone, epitestosterone, androsterone, etiocholanolone, dehydroepiandrosterone) in hair. After alkaline hydrolysis, the hair sample was extracted with diethyl ether, derivatized with a derivatization reagent (N-methyl-N-trimethylsilyl-trifluoroacetamid/iodotrime-thyisilane/DL-dithiothreitol, 1000:5:5, v/v/w) and detected using GC-MS/MS in the multiple-reaction monitoring mode. The one precursor/two product ion transitions for each anabolic steroids were monitored. The limits of detection for five endogenous anabolic steroids were in the range of 0.1 - 0.2 pg/mg. All analytes showed good linearity and the extraction recoveries were 74.6% - 104.5%. The inter-day and intra-day relative standard deviations (RSD) were less than 17.5%. This method has been applied to the analysis of testosterone, epitestosterone, androsterone, etiocholanolone, dehydroepiandrosterone in 80 Chinese hair samples. These data are the suitable references and the basis for the interpretation of the results from endogenous steroids abuse.
Shen
Min
M
Institute of Forensic Sciences, Ministry of Justice, Shanghai Key Laboratory of Forensic Medicine, Shanghai 200063, China. minshensfjd@hotmail.com
Xiang
Ping
P
Shen
Baohua
B
Wang
Mengye
M
D045930:Anabolic Agents / Q000032:analysis*; D019963:Clinical Chemistry Tests / Q000379:methods*; D004300:Doping in Sports; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D013256:Steroids / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D045930:Anabolic Agents; D013256:Steroids