title: [Evaluation of an anti-HIV-1/2 antibodies detection kit based on counting immunoassay in whole blood].
abstract: We evaluated a novel anti-human immunodeficiency virus type-1/2 (HIV-1/2) antibody detection kit for detecting anti-HIV-1/2 antibodies in whole blood based on the counting immunoassay (CIA) using an automated immunochemical analyzer (PAMIA-40i). This kit to detected all antibodies tested including those against HIV-1 subtype A to F, B/D in group M, HIV-1 group O, and HIV-2, and captured antibodies 4 to 7 days earlier than immunochoromatocraphic tests in the commercial seroconversion panel. In this study using 70 HIV-seropositive patients and 90 HIV-seronegative healthy individuals, both sensitivity and specificity were 100%. This automatic system using CIA for whole blood can be completed within 15 min and examine many samples simultaneously. This system used latex-counting technology and reliably detects the low-titer antibodies in whole blood.
authors.affiliation: AIDS Clinical Center, International Medical Center of Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Hachiya
authors.forename: Izumi
authors.initials: I
authors.lastname: Suetake
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kakuda
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oka
mesh_terms: D015415:Biomarkers / Q000097:blood; D015483:HIV Antibodies / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic