Robotic sample pretreatment-immunoassay determination of chlorpyrifos metabolite (TCP) in soil and fruit.
A fully automated method for leaching of TCP (3,5,6-trichloro-2-pyridinol, the major degradation product of the widely used chlorpyrifos insecticides) from soil and fruit with subsequent determination by ELISA is reported. The automation of the weighing and leaching steps enables unattended development of sample preparation. The determination of the target analyte, traditionally performed by liquid chromatography, has been substituted by specific immunoassay using a non-commercial monoclonal antibody which enables the determination of TCP within the range 0.01-7 ng 1(-1) with an R.S.D. of 8.6%.
Velasco-Arjona
A
A
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004 Córdoba, Spain.
Manclús
J J
JJ
Montoya
A
A
Luque de Castro
M D
MD