title: Surface characterization and cytocompatibility of three chitosan/polycation composite membranes for guided bone regeneration.
abstract: Guided bone regeneration is a promising surgical procedure for reconstructing bone defects. In this study, three chitosan/polycation composite membranes for guided bone regeneration are produced by blending chitosan with poly-L-lysine, polyethyleneimine, and poly-L-ornithine. For all composite membranes, the surface characteristics including surface topography, chemistry, and wettability are examined by atomic force microscopy, X-ray photoelectron spectroscopy, and contact angle assay. Their cytocompatibility is also evaluated with MC3T3-E1 osteoblast-like cells at cell, protein, and gene levels through cell biology assays, western blot, and RT-PCR analysis. On chitosan/poly-L-lysine composite membrane, MC3T3-E1 cells present well-developed cytoskeletal organization and significantly higher adhesion, proliferation, and differentiation than those on chitosan and the other two composite membranes. Furthermore, MC3T3-E1 cells on chitosan/poly-L-lysine membrane exhibit increased phosphorylation levels of focal adhesion kinase and extracellular signal-regulated kinase 1/2, and achieve an enhanced mRNA expression of fibronectin, Runx 2, RhoA, integrin alpha 5, and integrin beta1. From our results, we conclude that chitosan/ poly-L-lysine composite membrane possesses improved cytocompatibility with osteoblasts when compared to chitosan and holds potential for guided bone regeneration in the near future.
authors.affiliation: Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Zhenhuan
authors.initials: Z
authors.lastname: Zheng
authors.forename: Yujun
authors.initials: Y
authors.lastname: Wei
authors.forename: Gan
authors.initials: G
authors.lastname: Wang
authors.forename: Yandao
authors.initials: Y
authors.lastname: Gong
authors.forename: Xiufang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D001861:Bone Regeneration / Q000502:physiology*; D002412:Cations; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D048091:Guided Tissue Regeneration / Q000379:methods*; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D010316:Particle Size; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D002412:Cations; D008567:Membranes, Artificial; D048271:Chitosan