Inhibition of acrolein-stimulated MUC5AC production by fucoidan in human bronchial epithelial cells.
Fucoidan, a marine sulfated polysaccharide has both antithrombotic and anti-inflammatory effects. We determined the effect of fucoidan on MUC5AC expression in a human bronchial epithelial cell line, NCI-H292. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that fucoidan inhibited MUC5AC expression and protein secretion in cells stimulated with acrolein, a toxic aldehyde present in tobacco smoke. The activation of both nuclear factor-kappa B (NF-kappa B) and activator protein 1 (AP-1) are key steps in the transcriptional activation of MUC5AC. We found that the acrolein-mediated transactivation of MUC5AC was selectively dependent on AP-1 activation and was suppressed by fucoidan. Fucoidan-induced AP-1 inhibition and MUC5AC repression might be associated with fucoidan's protective effects against respiratory diseases.
Pokharel
Yuba Raj
YR
BK21 Project Team, College of Pharmacy, Chosun University, Gwangju, South Korea.
Yoon
Se Young
SY
Kim
Sang Kyum
SK
Li
Jian-Dong
JD
Kang
Keon Wook
KW
D000171:Acrolein / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D055271:Mucin 5AC / Q000096:biosynthesis*; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018808:Transcription Factor AP-1 / Q000378:metabolism
D000970:Antineoplastic Agents; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D016328:NF-kappa B; D011134:Polysaccharides; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D000171:Acrolein; C007789:fucoidan