Comparison of pyrolysis mass spectrometry with high performance liquid chromatography for the analysis of Cremophor EL in drugs and serum.
Polyoxyethyleneglycerol triricinoleate 35 (Cremophor EL: CrEL) is a solubilization agent for hydrophobic drugs. Recently, CrEL has shown some side effects in patients. In the present work, we introduce pyrolysis-mass spectrometry (Py-MS) for the determination of CrEL in drugs and blood samples. Mass to charge (m/z) values of 89 and 138 of CrEL and 3-nitroaniline (as internal standard), respectively, were used for quantitative measurements by selected ion monitoring (SIM) method. At a probe pyrolysis temperature range of 350-450 degrees C the results are highly reproducible. Limit of detection (LOD), linearity and relative standard deviation (R.S.D., n=5) were determined to be 1 ng ml(-1), 10 ng ml(-1)-100 mg ml(-1) and 1.3%, respectively. The results of Py-MS are compared with those obtained by high performance liquid chromatography (HPLC) and show that time for analysis, sensitivity and linearity are far better.
Ghassempour
Alireza
A
Chemical Research Center, Faculty of Science, Shahid Beheshti University, Evin, Tehran, Iran.
Nabid
Mohammad Reza
MR
Talebi
Mohammad
M
Entezami
Ali Akbar
AA