Flow-reversal flow injection analysis-II Determination of glucose with a double-pump system.
A new type of flow-injection procedure is proposed in which the samples are reversely pumped to the detector. In this procedure the injected samples are pumped into the reaction loop of a 6-way valve, then the valve is rotated to reverse the flow and the sample/reagent plug is pumped to the detector by another pump. The dispersion of the sample zone is low and the consumption of the reagent is very small. Therefore, its analytical potential for analysis with expensive reagents or long reaction times is high. The procedure has been applied to the determination of glucose in serum with an enzyme kit.
Toei
J
J
Biochemical Research Laboratory, Tosoh Co. Ltd., 2743-1, Hayakawa, Ayase-shi,Kanagawa 252, Japan.