title: Immuno-arrays for multianalyte analysis of chlorotriazines.
abstract: In this paper, a novel strategy for multicomponent analysis of two classes of pesticides such as triazines (atrazine and simazine) and phenoxyalkanoic acids (2,4-dichlorophenoxy acetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 4-chlorophenoxyacetic acid (CPOAc), phenoxyacetic acid (POAc)) employing immuno-arrays is demonstrated. The approach is based on cross-reactive arrays of specific antibody pairs coupled to chemometric pattern recognition. The monoclonal antibody pairs employed in this work (atrazine-simazine and 2,4-D) are specific towards a set of analytes and preclude a particular set of others present in the sample matrix. Antibody pairs of atrazine, simazine, and 2,4-D are used to discriminate and quantify analyte of interest. Atrazine was quantified in presence of trace concentration of simazine and that of 2,4-D. The combinatorial cross-reactivity of antibody pairs towards simazine, atrazine and 2,4-D is used to distinguish among different classes of analytes and their influence on the signal suppression in immuno-techniques. These sensors exclude recognition by carbamates such as carbaryl and carbofuran.
authors.affiliation: Pure and Applied Biochemistry, Lund University, Box 124, S-22100 Lund, Sweden.
authors.forename: Sunil
authors.initials: S
authors.lastname: Bhand
authors.forename: Ioana
authors.initials: I
authors.lastname: Surugiu
authors.forename: Anatoly
authors.initials: A
authors.lastname: Dzgoev
authors.forename: Kumaran
authors.initials: K
authors.lastname: Ramanathan
authors.forename: P V
authors.initials: PV
authors.lastname: Sundaram
authors.forename: Bengt
authors.initials: B
authors.lastname: Danielsson