Cloning and characterization of Sdga gene encoding alpha-subunit of heterotrimeric guanosine 5'-triphosphate-binding protein complex in Scoparia dulcis.
A homology-based cloning strategy yielded Sdga, a cDNA clone presumably encoding alpha-subunit of heterotrimeric guanosine 5'-triphosphate-binding protein complex, from leaf tissues of Scoparia dulcis. Phylogenetic tree analysis of G-protein alpha-subunits from various biological sources suggested that, unlike in animal cells, classification of Galpha-proteins into specific subfamilies could not be applicable to the proteins from higher plants. Restriction digests of genomic DNA of S. dulcis showed a single hybridized signal in Southern blot analysis, suggesting that Sdga is a sole gene encoding Galpha-subunit in this plant. The expression level of Sdga appeared to be maintained at almost constant level after exposure of the leaves to methyl jasmonate as analyzed by reverse-transcription polymerase chain reaction. These results suggest that Sdga plays roles in methyl jasmonate-induced responses of S. dulcis without a notable change in the transcriptional level.
Shite
Masato
M
Graduate School of Medicine and Pharmaceutical Science for Research, University of Toyama, Toyama, Japan.
Yamamura
Yoshimi
Y
Hayashi
Toshimitsu
T
Kurosaki
Fumiya
F
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036721:Scoparia / Q000235:genetics* / Q000378:metabolism
D000085:Acetates; D003517:Cyclopentanes; D044385:GTP-Binding Protein alpha Subunits; D054883:Oxylipins; C072239:methyl jasmonate