Essential role of SRC suppressed C kinase substrates in Schwann cells adhesion, spreading and migration.
Integrin-mediated substrate adhesion of endothelial cells leads to dynamic rearrangement of the actin cytoskeleton. Protein kinase C (PKC) stimulates reorganization of microfilaments and adhesion, while the responses of Schwann cells during adhesion and migration are unknown, so we examined the expression changes of SSeCKS and F-actin in Schwann cells after exposure to fibronectin. Src (sarcoma) suppressed C kinase substrate (SSeCKS) is a PKC substrate that may play an important role in regulating actin cytoskeleton. We found that SSeCKS was localized to focal adhesion sites soon after Schwann cells adhesion and that SSeCKS increased during the process of cell spreading. Using small interfering RNAs specific to SSeCKS, we showed that Schwann cells in which SSeCKS expression was inhibited reduced cellular adhesion, spreading and promoted cellular migration on fibronectin through reorganization of actin stress fibers and blocking formation of focal adhesions. These results demonstrated SSeCKS modulate Schwann cells adhesion, spreading and migration by reorganization of the actin cytoskeleton.
Yan
Meijuan
M
Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, People's Republic of China. ymz@ntu.edu.cn
Cheng
Chun
C
Jiang
Jing
J
Liu
Yonghua
Y
Gao
Ying
Y
Guo
Zhiqin
Z
Liu
Haiou
H
Shen
Aiguo
A
D054758:A Kinase Anchor Proteins / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002448:Cell Adhesion; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D003600:Cytosol / Q000378:metabolism; D005353:Fibronectins / Q000502:physiology; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D022001:Focal Adhesions / Q000502:physiology; D055785:Gene Knockdown Techniques; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D012583:Schwann Cells / Q000502:physiology*; D022502:Stress Fibers / Q000502:physiology; D019061:src-Family Kinases / Q000502:physiology*
D054758:A Kinase Anchor Proteins; C102361:Akap12 protein, rat; D018797:Cell Cycle Proteins; D005353:Fibronectins; D034741:RNA, Small Interfering; D051416:Focal Adhesion Protein-Tyrosine Kinases; D019061:src-Family Kinases