DNA migration regimes in hydrodynamic chromatography and slalom chromatography: evidence for a transition.
Recently, we hypothesized that DNA separation mechanisms in slalom chromatography (SC) and packed column hydrodynamic chromatography (HDC) could be connected and simultaneously observed 'J. Chromatogr. A 886 (2000) 1'. The present paper describes the migration of various circular and linear double-stranded DNAs in a chromatographic system using a C1 stationary phase and an acetonitrile-phosphate buffer mixture as a mobile phase. The migration dependence on the flow rate for the different species showed the relative contribution of both the SC and HDC separation mechanisms. A transition between the SC and HDC regimes was demonstrated for the first time. In addition, the data were analyzed in terms of polymer migration in a dense structure. Three different behaviors were distinguished in relation to the size and the compactness of the nucleic acid.
Peyrin
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Laboratoire de Chimie Analytique, UFR de Pharmacie, Universite Joseph Fourier, Domaine de la Merci, 38700 La Tronche Cedex, France.
Caron
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C
Garrel
C
C
Ravel
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A
Villet
A
A
Grosset
C
C
Favier
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A