Genetic characterization and polymorphisms for parentage testing of the Jeju horse using 20 microsatellite loci.
Genetic characterization of the Jeju horse (JH) was performed to construct a correct pedigree of the JH family. A total of 111 horses including 79 JH were genotyped using 20 microsatellite loci. The number of alleles varied from 5 to 11 (mean 7.45) in the JH. The observed heterozygosity and expected heterozygosity ranged from 0.293 to 0.891 and from 0.357 to 0.841, respectively. The polymorphic information contents (PIC) ranged from 0.335 to 0.816. AHT4, ASB2, ASB17, ASB23, CA425, HMS2, HMS3, HTG10, LEX3 and VHL20 loci had relatively high PIC values (> 0.7). The total exclusion probability (PE) of the 20 microsatellite loci was 0.9999 in the JH. These results provide basic information for developing an accurate pedigree and will be useful in making decisions regarding conservation of the JH.
Choi
Sung-Kyoon
SK
College of Veterinary Medicine, Kyungpook National University, Daegu, South Korea.
Cho
Chang-Yeon
CY
Yeon
Sung-Heum
SH
Cho
Byung-Wook
BW
Cho
Gil-Jae
GJ
D000483:Alleles; D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics; D006736:Horses / Q000235:genetics*; D018895:Microsatellite Repeats; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic
D004247:DNA