Collisionally induced fragmentation of [M-H](-) species of resveratrol and piceatannol investigated by deuterium labelling and accurate mass measurements.
Resveratrol (3,5,4'-trihydroxystilbene) and piceatannol (3,4,3',5'-tetrahydroxy-trans-stilbene) are phytoalexins present in red grapes and wines. In vitro studies have revealed that piceatannol blocks LMP2A, a viral protein-tyrosine kinase implicated in leukemia, non-Hodgkin's lymphoma and other diseases associated with the Epstein-Barr virus, and has an antimelanoma effect on human melanoma cells. Resveratrol has several beneficial effects on human health, such as anticancer, cardioprotection, antioxidant, inhibition of platelet aggregation and anti-inflammatory activity. In this investigation, the collisional behaviour of deprotonated resveratrol and piceatannol obtained under electrospray conditions is described. The mechanisms involved in the fragmentation pattern of [M-H](-) species of the two compounds were investigated by performing MS(n) experiments, deuterium labelling and accurate mass measurements.
Stella
Laura
L
CNR-ISTM, Sezione di Padova, Corso Stati Uniti 4, 35100 Padova.
De Rosso
Mirko
M
Panighel
Annarita
A
Vedova
Antonio Dalla
AD
Flamini
Riccardo
R
Traldi
Pietro
P
D003903:Deuterium; D041961:Deuterium Exchange Measurement / Q000379:methods*; D008970:Molecular Weight; D000077185:Resveratrol; D012717:Sesquiterpenes; D021241:Spectrometry, Mass, Electrospray Ionization; D013267:Stilbenes / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry; D000094162:Phytoalexins
D012717:Sesquiterpenes; D013267:Stilbenes; D013729:Terpenes; C041525:3,3',4,5'-tetrahydroxystilbene; D003903:Deuterium; D000077185:Resveratrol; D000094162:Phytoalexins