Determination of n-acetylmuramoyl-l-alanyl-d-isoglutamine in liposomes by HPLC.
A method using reversed-phase high-pressure liquid chromatography (with spectrometric detection at 218nm) is described for the determination in new pharmaceutical preparations (liposomes) of a new immunostimulating agent (N-acetylmuramoyl-l-alanyl-d-isoglutamine). Separation was achieved with a mu-bondapak column and phosphate buffer (pH 2.5)-methanol mixture (93:7 v/v) as eluent, at a flow-rate of 2 ml min . Sodium acetate was used as an internal standard. The detector response at 218 nm was linear in the range 10-170 mug ml . The method is simple and accurate.
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Laboratoires de contrôle de qualité, Pharmacie Centrale des Hôpitaux de Paris, 7, rue de Fer à Moulin, 75221 Paris Cedex 05, France.
Hamon
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Sponton
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Pradeau
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