title: Protocol for Plasmodium falciparum infections in mosquitoes and infection phenotype determination.
abstract: Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.
authors.affiliation: Malaria Research Institute, Bloomberg School of Public Health, John Hopkins University, USA.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Xi
authors.forename: Suchismita
authors.initials: S
authors.lastname: Das
authors.forename: Lindsey
authors.initials: L
authors.lastname: Garver
authors.forename: George
authors.initials: G
authors.lastname: Dimopoulos
mesh_terms: D000818:Animals; D001769:Blood; D018929:Cell Culture Techniques; D019411:Clinical Laboratory Techniques; D009033:Culicidae / Q000469:parasitology* / Q000821:virology; D004064:Digestive System / Q000821:virology; D006801:Humans; D016778:Malaria, Falciparum / Q000235:genetics* / Q000469:parasitology; D010641:Phenotype; D010963:Plasmodium falciparum / Q000302:isolation & purification; D019562:Viral Load