Composite film of carbon nanotubes and chitosan for preparation of amperometric hydrogen peroxide biosensor.
A new amperometric biosensor for hydrogen peroxide was developed based on cross-linking horseradish peroxidase (HRP) by glutaraldehyde with multiwall carbon nanotubes/chitosan (MWNTs/chitosan) composite film coated on a glassy carbon electrode. MWNTs were firstly dissolved in a chitosan solution. Then the morphology of MWNTs/chitosan composite film was characterized by field-emission scanning electron microscopy. The results showed that MWNTs were well soluble in chitosan and robust films could be formed on the surface. HRP was cross-linked by glutaraldehyde with MWNTs/chitosan film to prepare a hydrogen peroxide biosensor. The enzyme electrode exhibited excellent electrocatalytic activity and rapid response for H(2)O(2) in the absence of a mediator. The linear range of detection towards H(2)O(2) (applied potential: -0.2V) was from 1.67 x 10(-5) to 7.40 x 10(-4)M with correction coefficient of 0.998. The biosensor had good repeatability and stability for the determination of H(2)O(2). There were no interferences from ascorbic acid, glucose, citrate acid and lactic acid.
Qian
Lei
L
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Renming Street 5625, Changchun Jilin, China.
Yang
Xiurong
X