title: Chromatographic characterization of electrochemically generated technetium-HEDP skeletal imaging agents.
abstract: Reduction of pertechnetate [(99m)TcO(-)(4)] by controlled-potential coulometry in the presence of 1-hydroxy-1,1-ethanediphosphonate (HEDP) leads to the formation of (99m) Tc complexes which are suitable for bone imaging. The complex radiopharmaceutical mixtures can be separated into their respective components by anion-exchange high-performance liquid chromatography. The distribution of the complexes in the mixtures is dependent on the cell potential, pH, technetium concentration, and presence or absence of air. A single component formed in high yield and isolated from the mixture by HPLC was investigated as a potential bone-imaging agent. This particular complex is produced only in low yields when prepared by chemical reduction of (99m) TcO(-)(4). Thus electrochemistry shows promise in aiding in the development of a more efficacious bone-imaging agent by allowing selective generation of individual (99m) Tc-HEDP complexes.
authors.affiliation: Biomedical Chemistry Research Center, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, U.S.A.
authors.forename: R B
authors.initials: RB
authors.lastname: Scott
authors.forename: P J
authors.initials: PJ
authors.lastname: Schofield
authors.forename: E A
authors.initials: EA
authors.lastname: Deutsch
authors.forename: W R
authors.initials: WR
authors.lastname: Heineman