title: Association of p53 codon 72 polymorphism with liver metastases of colorectal cancers positive for p53 overexpression.
abstract: OBJECTIVE
  To evaluate the association between p53 codon 72 polymorphism (R72P) and the risk of colorectal liver metastases.
  
  
  METHODS
  The p53 R72P genotype was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 78 consecutive colorectal cancer patients with liver metastases and 214 age- and sex-matched cases with nonmetastatic colorectal cancer.
  
  
  RESULTS
  The R allele of the p53 R72P polymorphism was more frequently found in metastatic cases than in nonmetastatic cases (P=0.075). Carriers of the 72R allele had a 2.25-fold (95% CI (confidence interval)=1.05 to approximately 4.83) increased risk of liver metastases. On the stratification analysis, 72R-carrying genotype conferred a 3.46-fold (95% CI=1.02 to approximately 11.72) and a 1.05-fold (95% CI=0.36 to approximately 3.08) increased risk of liver metastases for p53 overexpression-positive and negative colorectal cancers, respectively.
  
  
  CONCLUSIONS
  These results demonstrate for the first time that the 72R allele of the p53 polymorphism has an increased risk for liver metastases in colorectal cancers positive for p53 overexpression.
authors.affiliation: Department of Pathology, No. 113 Hospital of People's Liberation Army, Ningbo 315040, China. zzzhu1170@yahoo.com
authors.forename: Zhong-Zheng
authors.initials: ZZ
authors.lastname: Zhu
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Ai-Zhong
authors.initials: AZ
authors.lastname: Wang
authors.forename: Hang-Ruo
authors.initials: HR
authors.lastname: Jia
authors.forename: Xia-Xiang
authors.initials: XX
authors.lastname: Jin
authors.forename: Xiang-Lei
authors.initials: XL
authors.lastname: He
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Hou
authors.forename: Guan-Shan
authors.initials: GS
authors.lastname: Zhu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000556:secondary*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics; D005260:Female; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000556:secondary*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53