Fluorimetric and phosphorimetric characteristics of several vitamins.
The fluorescence and phosphorescence characteristics of 14 vitamins were evaluated. The existence or absence of fluorescence and phosphorescence was compared with predictions based upon molecular structure. Analytically useful fluorescence signals were obtained for p-aminobenzoic acid, folic acid, calciferol, pyridoxine hydrochloride, riboflavine, alpha-tocopherol and vitamin A, whereas analytically useful phosphorescence signals were obtained for p-aminobenzoic acid, folic acid, niacinamide, pyridoxine hydrochloride, and alpha-tocopherol. All other vitamins either did not fluoresce or phosphoresce or could not be measured, owing to experimental difficulties. The complementary nature of fluorimetry and phosphorimetry is evident in these studies.
Aaron
J J
JJ
Department of Chemistry, University of Florida, Gainesville, Florida 32601, U.S.A.
Winefordner
J D
JD