RpoH(II) activates oxidative-stress defense systems and is controlled by RpoE in the singlet oxygen-dependent response in Rhodobacter sphaeroides.
Photosynthetic organisms need defense systems against photooxidative stress caused by the generation of highly reactive singlet oxygen ((1)O(2)). Here we show that the alternative sigma factor RpoH(II) is required for the expression of important defense factors and that deletion of rpoH(II) leads to increased sensitivity against exposure to (1)O(2) and methylglyoxal in Rhodobacter sphaeroides. The gene encoding RpoH(II) is controlled by RpoE, and thereby a sigma factor cascade is constituted. We provide the first in vivo study that identifies genes controlled by an RpoH(II)-type sigma factor, which is widely distributed in the Alphaproteobacteria. RpoH(II)-dependent genes encode oxidative-stress defense systems, including proteins for the degradation of methylglyoxal, detoxification of peroxides, (1)O(2) scavenging, and redox and iron homeostasis. Our experiments indicate that glutathione (GSH)-dependent mechanisms are involved in the defense against photooxidative stress in photosynthetic bacteria. Therefore, we conclude that systems pivotal for the organism's defense against photooxidative stress are strongly dependent on GSH and are specifically recognized by RpoH(II) in R. sphaeroides.
Nuss
Aaron M
AM
Institut für Mikrobiologie und Molekularbiologie, Heinrich-Buff-Ring 26, 35392 Giessen, Germany.
Glaeser
Jens
J
Klug
Gabriele
G
D012321:DNA-Directed RNA Polymerases / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D005816:Genetic Complementation Test; D006360:Heat-Shock Proteins / Q000235:genetics / Q000502:physiology*; D008027:Light; D016296:Mutagenesis; D018384:Oxidative Stress / Q000502:physiology*; D010788:Photosynthesis / Q000502:physiology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012242:Rhodobacter sphaeroides / Q000235:genetics / Q000502:physiology*; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*; D026082:Singlet Oxygen / Q000502:physiology*
D006360:Heat-Shock Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012808:Sigma Factor; C069774:heat-shock sigma factor 32; D026082:Singlet Oxygen; D012321:DNA-Directed RNA Polymerases