Electrophoretic separations of neuromediators on microfluidic devices.
In the present work, on-chip capillary electrophoresis for the separation of neuromediators is demonstrated. The influence of separation buffer (composition, pH, SDS additive), on-chip electrokinetic sample stacking, and surface pretreatment of the PDMS-PDMS and hybrid PDMS-glass devices on the electrokinetic characteristics of microfluidics (nu(eo), mu(eo), zeta) and separation performance of on-chip capillary electrophoresis of neuromediators have been investigated. It is demonstrated that for the effective separation of neuropeptides on elastomer-based microfluidic devices, on-chip sample stacking is necessary. Field-amplified sample stacking for electroosmotic flow supported on-chip separations of neuromediators and without special design of the sample injection scheme has been demonstrated. Electrophoretic separations of fluorescently labeled analytes have been achieved within tens of seconds at injection volumes of about 110pL, with plate numbers varying from <1000 to approximately 22,000. These results demonstrate that on-chip separation methods with hybrid PDMS-glass devices are perspective for the analysis of (neuro)peptides in small volumes.
Mourzina
Yulia
Y
Institute of Thin Films and Interfaces, Center of Nanoelectronic Systems for Information Technology, Research Center Jülich, 52425 Jülich, Germany.
Kalyagin
Dmitry
D
Steffen
Alfred
A
Offenhäusser
Andreas
A