Spectrofluorometric determination of thiopurines-I 6-Thioguanine.
6-Thioguanine is oxidized to the corresponding S-oxide by reaction with hydrogen peroxide in 50% acetic acid at 50 degrees for 5 min. The S-oxide is highly fluorescent and a rapid and sensitive fluorometric method of determination of thioguanine at the ng ml level in water and mug ml level in blood serum and urine has been developed on the basis of this reaction. The method has been used for the determination of thioguanine in blood and urine samples.
Thomas
A D
AD
Department of Chemistry, University of Aberdeen, Old Aberdeen, Scotland.