Creatinine determination in urine samples by batchwise kinetic procedure and flow injection analysis using the Jaffé reaction: chemometric study.
The classical Jaffé reaction for the determination of creatinine in urine samples is tested. A comparative study of the main analytical characteristics focussed to minimize the bias error and improve the precision, for the batchwise and flow injection (FI) methods is realized. Also, the effect of the albumin concentration in the determination of creatinine has been studied. Different analytical signals were studied. Absorbance increments at different times permit to estimate the creatinine concentration free from bias error in urine by the batchwise method using the calibration graph obtained with creatinine standards and no measurement of the blank solution is needed. The lineal interval was 0.92-50 mg l(-1) and seven samples can be processed per hour by an operator. No previous treatment of the urine sample is necessary. The FI method provides also good results. The lineal interval was 30-100 mg l(-1) and the sample rate was around 20 samples per hour. If increased albumin levels are detected in the urine, standard addition method or the calibration graphs with standards in presence of albumin are needed in order to obtain accurate results when FI method is employed. The obtained accuracy of the both methods allows its application as diagnostic tool to establish the urinary creatinine levels.
Campins Falcó
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Departament de Quimica Analitica, Facultad de Quimica Universitat de Valencia, C/Dr. Moliner 50, E46100-Burjassot, 46100 Valencia, Spain.
Tortajada Genaro
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LA
Meseger Lloret
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S
Blasco Gomez
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F
Sevillano Cabeza
A
A
Molins Legua
C
C