Protocol for RNAi assays in adult mosquitoes (A. gambiae).
Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.
Garver
Lindsey
L
Malaria Research Institute, Bloomberg School of Public Health, John Hopkins University, USA.
Dimopoulos
George
G
D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000469:parasitology; D007267:Injections / Q000379:methods; D008288:Malaria / Q000635:transmission; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000008:administration & dosage*; D013909:Thorax
D012330:RNA, Double-Stranded