Lipopeptides produced by a soil Bacillus megaterium strain.
A soil microorganism identified as Bacillum megaterium was found to produce several antibiotics substances after growth for 20 h at 37 degrees C in a mineral culture medium. Analysis both by electron spray ionization (ESI) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) identified these substances as lipopeptides. Predominant peaks at m/z 1,041 and m/z 1,065 revealed ions which are compatible with surfactins and lichenysins, respectively. Two other ions m/z 1,057 and m/z 1,464 were further studied by collision-induced dissociation (CID) unveiling an iturin A at the first and fengycins A and B at the second m/z peaks. The CID spectrum of the m/z 1,464 ion also suggests the existence of fengycins A and B variants in which Ile was changed to Val in the position 10 of the peptide moiety. Raw mixtures of all these compounds were also assayed for antibiotic features. The data enlighten the unusual diversity of the lipopeptide mixture produced by a sole Bacillus species.
Pueyo
Manuel Troyano
MT
Departamento de Bioquimica, Instituto de Quimica, Universidade de São Paulo, São Paulo, SP, Brazil. matpueyo@iq.usp.br
Bloch
Carlos
C
Carmona-Ribeiro
Ana Maria
AM
di Mascio
Paolo
P
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001410:Bacillus megaterium / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry; D003470:Culture Media; D055666:Lipopeptides / Q000096:biosynthesis* / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010456:Peptides, Cyclic; D012988:Soil Microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013500:Surface Tension; D013501:Surface-Active Agents / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D055666:Lipopeptides; D010456:Peptides, Cyclic; D013501:Surface-Active Agents; C013579:iturin A