SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues.
SNP-minisequencing has become common in forensic genetics, especially for analysing degraded or low copy number DNA (LCN DNA). The aim of this study was to examine the usefulness of five SNP (single nucleotide polymorphism) markers for analyzing degraded and LCN DNA recovered from archival samples. DNA extractions of eight formalin-fixed paraffin-embedded (FFPE) tissues were performed and DNA fragments were amplified in one multiplex PCR (polymerase chain reaction). SNPs were identified in a minisequencing reaction and a gel electrophoresis in ABI Prism 377 Sequencer. The research confirmed the usefulness of SNP-minisequencing for analysing FFPE tissues.
Babol-Pokora
Katarzyna
K
Department of Forensic Medicine, Medical University of Lodz, Łódź, Poland. pokorak@gmail.com
Berent
Jarosław
J
D004247:DNA / Q000235:genetics*; D006801:Humans; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
D004247:DNA