Generating arrays with high content and minimal consumption of functional membrane proteins.
This paper introduces a widely accessible and straightforward technique for fabricating membrane protein arrays. This technique employs topographically patterned agarose gels to deliver various membrane preparations to glass substrates in a rapid and parallel fashion. It can fabricate more than 30 identical copies of a membrane protein array while requiring only femtomoles of protein. Taking advantage of on-stamp preconcentration, it is able to pattern arrays of multilayered membrane spots with more than 20-fold increased content of membrane proteins compared to existing methods.
Majd
Sheereen
S
Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109-2110, USA.
Mayer
Michael
M
D006801:Humans; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D011950:Receptors, Cholinergic / Q000032:analysis* / Q000737:chemistry*; D013925:Thromboplastin / Q000032:analysis / Q000737:chemistry
D011950:Receptors, Cholinergic; D013925:Thromboplastin