Thin-layer chromatography of laser dyes and dye analogs with cyclodextrins in the mobile phase.
The addition of beta-cyclodextrin and substituted hydroxyethyl and hydroxypropyl-beta-cyclodextrins to aqueous urea-containing mobile phases, can be used to enhance the migration of various laser dyes on thin layer chromatography. Selected laser dyes from the coumarin, rhodamine and bimane families were examined, as well as a number of dye analogs. Silica gel, polyamide and C18 reverse phase plates were utilized. Overall, the substituted hydroxypropyl-beta-cyclodextrins appeared to be the most effective for increasing dye migration.
Politzer
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Department of Chemistry, Xavier University, New Orleans, Louisiana 70125, U.S.A.
Crago
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Amos
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Mitchell
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Hollin
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