Application of simplified complementary tristimulus colorimetry to chemical kinetics in solution-II Determination of rate constants of consecutive irreversible first-order reactions.
A simple but highly reproducible method based on simplified complementary tristimulus colorimetry is proposed for determination of the rate constants of two consecutive irreversible first-order reactions. For the binary system the complementary colour points can be calculated in the same manner as in conventional tristimulus colorimetry. Hence the mole fractions of the three reacting species can be calculated as a function of time. Application of the method is illustrated by the assay of xanthine oxidase with hypoxanthine.
Tawa
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R
Department of Analytical Chemistry, Kyoto College of Pharmacy Yamashina-ku, Kyoto 607, Japan.
Hirose
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S