title: Influence of nanofibers on the growth and osteogenic differentiation of stem cells: a comparison of biological collagen nanofibers and synthetic PLLA fibers.
abstract: The aim of this study was to compare biological collagen I (ColI) and synthetic poly-(L: -lactide) (PLLA) nanofibers concerning their stability and ability to promote growth and osteogenic differentiation of human mesenchymal stem cells in vitro. Matrices were seeded with human stem cells and cultivated over a period of 28 days under growth and osteoinductive conditions and analyzed during the course. During this time the PLLA nanofibers remained stable while the presence of cells resulted in an attenuation of the ColI nanofiber mesh. Although there was a tendency for better growth and osteoprotegerin production of stem cells when cultured on collagen nanofibers, there was no significant difference compared to PLLA nanofibers or controls. The gene expression of alkaline phosphate, osteocalcin and collagen I diminished in the initial phase of cultivation independent of the polymer used. In the case of PLLA fibers, this gene expression normalized during the course of cultivation, whereas the presence of collagen nanofibers resulted in an increased gene expression of osteocalcin and collagen during the course of the experiment. Taken together the PLLA fibers were easier to produce, more stable and did not compromise growth and differentiation of stem cells over the course of experiment. On the other hand, collagen nanofibers supported the differentiation process to some extent. Nevertheless, the need for fixation as well as the missing stability during cell culture requires further work.
authors.affiliation: Department of Orthopedics, University of Marburg, Baldingerstrasse, 35043, Marburg, Germany. schofer@med.uni-marburg.de
authors.forename: Markus Dietmar
authors.initials: MD
authors.lastname: Schofer
authors.forename: Ulrich
authors.initials: U
authors.lastname: Boudriot
authors.forename: Christina
authors.initials: C
authors.lastname: Wack
authors.forename: Irini
authors.initials: I
authors.lastname: Leifeld
authors.forename: Christian
authors.initials: C
authors.lastname: Gräbedünkel
authors.forename: Roland
authors.initials: R
authors.lastname: Dersch
authors.forename: Markus
authors.initials: M
authors.lastname: Rudisile
authors.forename: Joachim Heinz
authors.initials: JH
authors.lastname: Wendorff
authors.forename: Andreas
authors.initials: A
authors.lastname: Greiner
authors.forename: Jürgen Rudolf Josef
authors.initials: JR
authors.lastname: Paletta
authors.forename: Susanne
authors.initials: S
authors.lastname: Fuchs-Winkelmann
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001672:Biocompatible Materials; D002452:Cell Count; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis; D053244:Osteoprotegerin / Q000378:metabolism; D011091:Polyesters; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D053244:Osteoprotegerin; D011091:Polyesters; C506030:TNFRSF11B protein, human; D015675:Osteocalcin; C033616:poly(lactide); D000469:Alkaline Phosphatase