title: Two-photon imaging of cellular activities in oxygen sensing tissues.
abstract: The cellular oxygen sensing system of the body ensures appropriate adaptation of cellular functions toward hypoxia by regulating gene expression and ion channel activity. Two-photon laser microscopy is an ideal tool to study and prove the relevance of the molecular mechanisms within oxygen sensing pathways on the cellular and complex tissue or organ level. Images of hypoxia inducible factor 1 (HIF-1) subunit nuclear mobility and protein-protein interaction in living cells, of hypoxia-induced changes in membrane potential and intracellular calcium of live ex vivo carotid bodies as well as of rat kidney proximal tubulus function in vivo, will be shown.
authors.affiliation: Institut für Physiologie, Universität Duisburg-Essen, Hufelandstrasse 55, D-45122 Essen, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Wotzlaw
authors.forename: Utta
authors.initials: U
authors.lastname: Berchner-Pfannschmidt
authors.forename: Joachim
authors.initials: J
authors.lastname: Fandrey
authors.forename: Helmut
authors.initials: H
authors.lastname: Acker
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002344:Carotid Body / Q000166:cytology / Q000378:metabolism; D015687:Cell Hypoxia; D002460:Cell Line; D002477:Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000378:metabolism; D036641:Microscopy, Fluorescence, Multiphoton; D010100:Oxygen / Q000378:metabolism*
chemical_list: D051793:Hypoxia-Inducible Factor 1; D010100:Oxygen; D002118:Calcium