Inclusion of metal micro-particles into poly(pyrrolylalkylammonium) films containing an enzyme for bioelectrocatalysis: a preliminary study.
The electrocatalytic detection of products issued from enzymatic reactions occuring at biosensor interfaces can be improved owing to metal microparticle dispersions. This contribution describes the faesability of this concept for the modification poly(pyrrolylalkylammonium) films containing an enzyme through the inclusion of platinum. A model enzyme, glucose oxidase (GOx) was employed to demonstrate the reliability of this approach through the design of an amperometric glucose biosensors. The obtained composite assemblies were characterised regarding their sensing capabilities toward glucose. Platinum particles incorporation was operated within the biofilms to obtain a suitable sensitivity of the biosensor to glucose at a lower operational oxidation potential (eg 0.5 V/SCE) than the oxidation potential generally observed for hydrogen peroxide detection onto carbon electrodes. The incorporation procedure of platinum aggregates has been optimised to design biosensors exhibiting a sensitivity of about 8 mA. M(-1) cm(-2).
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Laboratoire des Structures et Propriétés d'Architectures Moléculaires, UMR 5819, Université Joseph Fourier Grenoble 1-CNRS-CEA, DRFMC/SI3M/EMSI, CEA, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France.