title: Development of an immunochromatographic test to detect antibodies against recombinant Em18 for diagnosis of alveolar echinococcosis.
abstract: An immunochromatographic test (ICT) for the rapid detection of antibodies to Echinococcus multilocularis was developed. The ICT showed a sensitivity of 94% and a specificity of 95.4%. High degrees of agreement were observed between the ICT and an enzyme-linked immunosorbent assay (kappa = 0.93) and between the ICT and immunoblot analysis (kappa = 0.97). It is expected that the ICT developed in this study will be useful for the serodiagnosis of alveolar echinococcosis.
authors.affiliation: Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1, Asahikawa, 078-8510 Hokkaido, Japan. yasusako@asahikawa-med.ac.jp
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Kenta
authors.initials: K
authors.lastname: Fukuda
authors.forename: Yukuharu
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002845:Chromatography / Q000379:methods; D004445:Echinococcosis, Pulmonary / Q000175:diagnosis*; D048210:Echinococcus multilocularis / Q000276:immunology* / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007158:Immunologic Techniques; D010274:Parasitology / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth