title: Human interferon-lambda3 is a potent member of the type III interferon family.
abstract: Type III interferon (IFN) or IFN-lambda is a recently discovered family of IFNs that signal through the same downstream transcription factors as type I IFN but use a separate receptor complex composed of the IL-10R2 and the unique IFN-lambdaR1 receptor chains. We have established a simple and efficient expression system to produce highly pure and active IFN-lambda of the three human IFN-lambda isoforms (IFN-lambda1, -lambda2 and -lambda3) and used this to compare the biological activity of the different IFN-lambda subtypes. Surprisingly, we found IFN-lambda3 to possess the highest specific activity of the human IFN-lambda subtypes, exhibiting a twofold higher activity than IFN-lambda1 and a 16-fold higher activity than IFN-lambda2. Furthermore, in comparison with the commercially available preparations of IFN-lambda1 and -lambda2, we found our IFN-lambda preparation to be superior in activity.
authors.affiliation: Department of Molecular Biology, Centre for Structural Biology, University of Aarhus, Aarhus C, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Dellgren
authors.forename: H H
authors.initials: HH
authors.lastname: Gad
authors.forename: O J
authors.initials: OJ
authors.lastname: Hamming
authors.forename: J
authors.initials: J
authors.lastname: Melchjorsen
authors.forename: R
authors.initials: R
authors.lastname: Hartmann
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D004285:Dogs; D015870:Gene Expression; D006801:Humans; D007372:Interferons; D053710:Interleukin-10 Receptor beta Subunit / Q000235:genetics / Q000276:immunology*; D007378:Interleukins / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D017471:Receptors, Interferon / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D000096743:Interferon gamma Receptor; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D053710:Interleukin-10 Receptor beta Subunit; D007378:Interleukins; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D000096743:Interferon gamma Receptor; C000624353:interferon-lambda, human; D000095022:Interferon Lambda; C081846:IL10RB protein, human