title: New method for bacterial load measurement during hydro-debridement of cutaneous ulcer.
abstract: OBJECTIVE
  Based on their experience using Versajet for debridement of chronic wounds, the Authors set up a study protocol to verify whether the hydro-surgical cleansing could offer the possibility of taking tissue specimens suitable for diagnostic microbiological evaluation. The aims of the study were the following:
  
  
  OBJECTIVE
  To evaluate the efficacy of hydro-surgery in detecting the presence of microorganisms and measuring their load, as an alternative to conventional tissue sampling methods; SECONDARY PURPOSE: To set up an easier and less invasive diagnostic modality than surgical biopsy, even though likewise significant.
  
  
  RESULTS
  The results of this study show that tissue specimen collection by hydro-aspiration using Versajet is comparable to biopsy sampling (and in some cases it can be even more reliable); moreover, it is not more time-consuming and is certainly less invasive. Compared to surgical biopsy, with such a method a greater amount of tissue may be collected; moreover, tissue specimens can be taken from a broader surface or, depending on the needs, from a more focused area on the margin or at the bottom of the wound.
authors.affiliation: Seconda Università degli Studi di Napoli, Dipartimento di Scienze Anestesiologiche, Chirurgiche e dell'Emergenza.
authors.forename: Marco
authors.initials: M
authors.lastname: Apperti
authors.forename: Adriana
authors.initials: A
authors.lastname: Di Lucia
authors.forename: Luigi
authors.initials: L
authors.lastname: Goffredi
authors.forename: Pasquale
authors.initials: P
authors.lastname: Federico
authors.forename: Eddy
authors.initials: E
authors.lastname: Mattera
authors.forename: Andrea
authors.initials: A
authors.lastname: Masella
authors.forename: Mattia Davide
authors.initials: MD
authors.lastname: Della Rocca
authors.forename: Sarah
authors.initials: S
authors.lastname: Apperti
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001424:Bacterial Infections / Q000175:diagnosis* / Q000188:drug therapy; D001706:Biopsy / Q000379:methods; D003646:Debridement / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D006875:Hydrotherapy / Q000379:methods*; D007871:Leg Ulcer / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology / Q000601:surgery*; D012189:Retrospective Studies; D012883:Skin Ulcer / Q000382:microbiology / Q000601:surgery; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents