The time-course analysis of gene expression during wound healing in mouse skin.
RNA analysis has been applied to forensic work to determine wound age. We investigated mRNA expression using quantitative RT-PCR of ten genes, including c-fos, fosB, mitogen-activated protein kinase phosphatase-1 (MKP-1), CD14, chemokine (C-C motif) ligand 9 (CCL9), placenta growth factor (PlGF), mast cell protease-5 (MCP-5), growth arrest specific 5 (Gas5), beta-2 microglobulin (B2M) and major urinary protein-1 (MUP-1), in terms of repair response in adult mice. The expression level of c-fos, fosB and MKP-1 transcripts increased drastically, peaked within 1h, and that of the CD14 and CCL9 transcripts peaked from 12 to 24h. An increase in PlGF and MCP-5 mRNA appeared on about day 5. Gas5, B2M and MUP-1 transcripts showed no significant change. Each gene had differentially expressional patterns with time-course. Our result implied that the observation of the 7 genes in wounded skin could serve to aid in the accurate diagnosis of wound age.
Kagawa
Shinichiro
S
Department of Forensic Pathology and Science, Unit of Social Medicine, Course of Medical and Dental Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Sakamoto, Nagazaki, Japan.
Matsuo
Aya
A
Yagi
Yoichi
Y
Ikematsu
Kazuya
K
Tsuda
Ryouichi
R
Nakasono
Ichiro
I
D000818:Animals; D019742:Chemokines, CC / Q000235:genetics / Q000378:metabolism; D054638:Dual Specificity Phosphatase 1 / Q000235:genetics / Q000378:metabolism; D049429:Forensic Pathology; D015870:Gene Expression; D016762:Genes, fos / Q000235:genetics; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism; D019402:Macrophage Inflammatory Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018945:Monocyte Chemoattractant Proteins / Q000235:genetics / Q000378:metabolism; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020537:RNA, Small Nucleolar / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000293:injuries / Q000378:metabolism*; D013997:Time Factors; D014945:Wound Healing / Q000235:genetics*; D001613:beta 2-Microglobulin / Q000235:genetics / Q000378:metabolism
C491224:Ccl12 protein, mouse; C095218:Ccl9 protein, mouse; D019742:Chemokines, CC; C483819:Fosb protein, mouse; D018950:Lipopolysaccharide Receptors; D019402:Macrophage Inflammatory Proteins; D018945:Monocyte Chemoattractant Proteins; C000608037:Pgf protein, mouse; D011257:Pregnancy Proteins; D011506:Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D020537:RNA, Small Nucleolar; D001613:beta 2-Microglobulin; C109857:growth arrest specific transcript 5; C035737:major urinary proteins; D000072483:Placenta Growth Factor; D054638:Dual Specificity Phosphatase 1; C517020:Dusp1 protein, mouse