Expression of basal lamina components by Schwann cells cultured on poly(lactic acid) (PLLA) and poly(caprolactone) (PCL) membranes.
The present in vitro study investigated the expression of basal lamina components by Schwann cells (SCs) cultivated on PCL and PLLA membranes prepared by solvent evaporation. Cultures of SCs were obtained from sciatic nerves from neonatal Sprague Dawley rats and seeded on 24 well culture plates containing the polymer membranes. The purity of the cultures was evaluated with a Schwann cell marker antibody (anti-S-100). After one week, the cultures were fixed and processed for immunocytochemistry by using antibodies against type IV collagen, laminin I and II. Positive labeling against the studied molecules was observed, indicating that such biomaterials positively stimulate Schwann cell adhesion and proliferation. Overall, the present results provide evidence that membrane-derived biodegradable polymers, particularly those derived from PLLA, are able to provide adequate substrate and stimulate SCs to produce ECM molecules, what may have in turn positive effects in vivo, influencing the peripheral nerve regeneration process.
Pierucci
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Department of Anatomy, Laboratory of Nerve Regeneration, Institute of Biology, University of Campinas--UNICAMP, CP 6109, Campinas, SP CEP 13083-970, Brazil.
Duek
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de Oliveira
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D000818:Animals; D000831:Animals, Newborn; D001485:Basement Membrane / Q000378:metabolism*; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism*
D001672:Biocompatible Materials; D016326:Extracellular Matrix Proteins; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D019344:Lactic Acid; C033616:poly(lactide)