title: Determination of l-malate in wine by flow-injection with co-immobilized malate dehydrogenase/oxaloacetate decarboxylase.
abstract: A method for determination of l-malate in wine is described. Malate dehydrogenase and oxaloacetate decarboxylase were immobilized on poly (vinyl alcohol) beads and incorporated in a flow-injection system with fluorescence detection. Sample solution (50 mul) was injected into the carrier stream [4mM NAD(+) in glycine buffer (pH 10.0)]. The fluorescence based on the NADH formed could be directly related to the amount of malate. The calibration graph was linear over the range 0.4-300muM. The detection limit was 0.2muM. Sampling throughout was 25 samples/hr.
authors.affiliation: Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu 400, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kiba
authors.forename: M
authors.initials: M
authors.lastname: Oguchi
authors.forename: M
authors.initials: M
authors.lastname: Furusawa