title: On-line determination of antimony(III) and antimony(V) in liver tissue and whole blood by flow injection - hydride generation - atomic absorption spectrometry.
abstract: A new analytical procedure for the speciation of antimony in liver tissues is presented here. For this purpose, a flow injection system has been developed for the treatment of samples and the determination of antimony by hydride generation - atomic absorption spectrometry. The method involves the sequential and the on-line extraction of antimony(III) and antimony(V) from solid lyophilized blood and hamsters liver tissues, with 1.5 mol l(-1) acetic acid and 0.5 mol l(-1) sulfuric acid for Sb(III) and Sb(V), respectively. Reduction of Sb(V) to Sb(III) for stibine generation is effected by the on-line pre-reduction with l-cysteine. The linear ranges were 2.5-20 and 1.0-25 mug l(-1) of Sb(III) and Sb(V), respectively. The detection limits (3sigma) were 1.0 mug l(-1) for Sb(III) and 0.5 mug l(-1) for Sb(V). The relative standard deviation values for fifteen independent measurements were 2.1 and 1.8% for Sb(III) and Sb(V), respectively. The recovery studies performed with samples of cattle liver provided results from 98 to 100% for Sb(III) and from 100 to 103% for Sb(V) for samples spiked with single species. For samples spiked with both Sb(III) and Sb(V), the recovery varied from 97 to 103% for Sb(III) and from 101 to 103% for Sb(V).
authors.affiliation: Facultad de Ciencias, IVAIQUIM (Venezuelan Andean Institute for Chemical Research), Universidad de Los Andes, La Hechicera, Merida 5101-A, Venezuela.
authors.forename: Y
authors.initials: Y
authors.lastname: Petit de Peña
authors.forename: O
authors.initials: O
authors.lastname: Vielma
authors.forename: J L
authors.initials: JL
authors.lastname: Burguera
authors.forename: M
authors.initials: M
authors.lastname: Burguera
authors.forename: C
authors.initials: C
authors.lastname: Rondón
authors.forename: P
authors.initials: P
authors.lastname: Carrero