title: Proteomic analysis of secreted membrane vesicles of archaeal Sulfolobus species reveals the presence of endosome sorting complex components.
abstract: The crenarchaea Sulfolobus acidocaldarius, S. solfataricus and S. tokodaii, release membrane vesicles into the medium. These membrane vesicles consist of tetraether lipids and are coated with an S-layer. A proteomic analysis reveals the presence of proteins homologous to subunits of the eukaryotic endosomal sorting complex required for transport (ESCRT). Immunodetection of one of these homologs suggest a cell surface localization in intact cells. These data suggest that the membrane vesicles in Sulfolobus sp. emerge from a specific budding process with similarity to the endosomal sorting pathway.
authors.affiliation: Department of Molecular Microbiology Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
authors.forename: Albert F
authors.initials: AF
authors.lastname: Ellen
authors.forename: Sonja-Verena
authors.initials: SV
authors.lastname: Albers
authors.forename: Wim
authors.initials: W
authors.lastname: Huibers
authors.forename: Angela
authors.initials: A
authors.lastname: Pitcher
authors.forename: Cedric F V
authors.initials: CF
authors.lastname: Hobel
authors.forename: Heinz
authors.initials: H
authors.lastname: Schwarz
authors.forename: Mihaela
authors.initials: M
authors.lastname: Folea
authors.forename: Stefan
authors.initials: S
authors.lastname: Schouten
authors.forename: Egbert J
authors.initials: EJ
authors.lastname: Boekema
authors.forename: Bert
authors.initials: B
authors.lastname: Poolman
authors.forename: Arnold J M
authors.initials: AJ
authors.lastname: Driessen
mesh_terms: D019843:Archaeal Proteins / Q000378:metabolism*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D011992:Endosomes / Q000378:metabolism*; D008854:Microscopy, Electron; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016944:Sulfolobus / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins