title: Hepatitis C virus infection protein network.
abstract: A proteome-wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view of the cellular infection. A total of 314 protein-protein interactions between HCV and human proteins was identified by yeast two-hybrid and 170 by literature mining. Integration of this data set into a reconstructed human interactome showed that cellular proteins interacting with HCV are enriched in highly central and interconnected proteins. A global analysis on the basis of functional annotation highlighted the enrichment of cellular pathways targeted by HCV. A network of proteins associated with frequent clinical disorders of chronically infected patients was constructed by connecting the insulin, Jak/STAT and TGFbeta pathways with cellular proteins targeted by HCV. CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins.
authors.affiliation: IMAP Team, Inserm Unit 851, Lyon, France.
authors.forename: B
authors.initials: B
authors.lastname: de Chassey
authors.forename: V
authors.initials: V
authors.lastname: Navratil
authors.forename: L
authors.initials: L
authors.lastname: Tafforeau
authors.forename: M S
authors.initials: MS
authors.lastname: Hiet
authors.forename: A
authors.initials: A
authors.lastname: Aublin-Gex
authors.forename: S
authors.initials: S
authors.lastname: Agaugué
authors.forename: G
authors.initials: G
authors.lastname: Meiffren
authors.forename: F
authors.initials: F
authors.lastname: Pradezynski
authors.forename: B F
authors.initials: BF
authors.lastname: Faria
authors.forename: T
authors.initials: T
authors.lastname: Chantier
authors.forename: M
authors.initials: M
authors.lastname: Le Breton
authors.forename: J
authors.initials: J
authors.lastname: Pellet
authors.forename: N
authors.initials: N
authors.lastname: Davoust
authors.forename: P E
authors.initials: PE
authors.lastname: Mangeot
authors.forename: A
authors.initials: A
authors.lastname: Chaboud
authors.forename: F
authors.initials: F
authors.lastname: Penin
authors.forename: Y
authors.initials: Y
authors.lastname: Jacob
authors.forename: P O
authors.initials: PO
authors.lastname: Vidalain
authors.forename: M
authors.initials: M
authors.lastname: Vidal
authors.forename: P
authors.initials: P
authors.lastname: André
authors.forename: C
authors.initials: C
authors.lastname: Rabourdin-Combe
authors.forename: V
authors.initials: V
authors.lastname: Lotteau
mesh_terms: D016174:Hepacivirus / Q000378:metabolism / Q000502:physiology; D006526:Hepatitis C / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins