[Simultaneous analysis of ten anabolic steroids in blood plasma using high performance liquid chromatography].
A method for the simultaneous separation and determination of ten anabolic steroids in blood plasma using high performance liquid chromatography (HPLC) was established. An RP-C18 column was used as the analytical column, and the mixture of acetonitrile and water was used as the mobile phase with gradient elution according to the characteristics of the analytes. The analytes were detected at the adjustable wavelengths ranging from 194 to 290 nm. Under the optimal conditions, ten compounds were separated within 10 min. The detection limits were in the range of 0.01 - 0.10 microg/mL. The spiked recoveries of standards in a rabbit plasma sample were from 70.3% to 120%. Methandriol was injected into the ear meridian of a rabbit, and then the anabolic steroid methandriol in the plasma was successfully detected with the established method. The results show that the method is feasible, rapid, simple and accurate.
Zhang
Lan
L
Key Laboratory of Analysis and Detection for Food Safety, Ministry of Education, Sport Science Research Center, Fuzhou University, Fuzhou 350002, China. zlan@fzu.edu.cn
Chen
Jinfeng
J
Tong
Ping
P
Li
Tianlin
T
D045930:Anabolic Agents / Q000097:blood* / Q000302:isolation & purification; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004300:Doping in Sports; D057230:Limit of Detection; D016014:Linear Models; D011817:Rabbits; D013256:Steroids / Q000097:blood* / Q000302:isolation & purification; D013997:Time Factors
D045930:Anabolic Agents; D013256:Steroids