title: Induction of type III secretion by cell-free Chlamydia trachomatis elementary bodies.
abstract: Chlamydiae secrete type III effector proteins at two distinct stages of their developmental cycle. Elementary bodies (EBs) secrete at least one pre-formed effector protein, Tarp, across the host plasma membrane from an extracellular location. Once internalized, a set of newly transcribed proteins are secreted to modify the inclusion membrane. In an effort to better understand the triggers for chlamydial type III secretion and develop means to identify new effectors, we investigated various inducers of T3SS in other Gram-negative bacterial systems to determine if they were able to activate chlamydial type III secretion from EBs using Tarp secretion as an indicator of activation. Chlamydial EBs are induced to secrete Tarp by exposure to FBS, BSA, or sphingolipid and cholesterol-rich liposomes (SCRLs). The induction by FBS and BSA, but not SCRL, is enhanced in the presence of the calcium-chelator, EGTA. This secretion was temperature dependent and inhibited by paraformaldehyde fixation of the EBs.
authors.affiliation: Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, 903 South Fourth Street, NIAID, NIH, Hamilton, MT 59840, USA.
authors.forename: Wendy P
authors.initials: WP
authors.lastname: Jamison
authors.forename: Ted
authors.initials: T
authors.lastname: Hackstadt
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D021381:Protein Transport; D013696:Temperature
chemical_list: D001426:Bacterial Proteins