title: [A simple method for assessment of RNA integrity in laser capture microdissection samples].
abstract: OBJECTIVE
  To develop a simple method for assessment of RNA integrity in laser capture microdissection (LCM) samples.
  
  
  METHODS
  The total RNA were isolated from the LCM samples and the sections before and after microdissection and examined by agarose gel electrophoresis. Real-time PCR was employed to assess the RNA from LCM samples, and the quantity of RNA was theoretically estimated according to the average total RNA product in mammalian cells (10 ng/1000 cells).
  
  
  RESULTS
  When the total RNA from the sections before and after microdissection was intact, the RNA from LCM samples also had good quality, and the 28S and 18S rRNAs were visualized by ethidium bromide staining. Real-time PCR also showed good RNA quality in the LCM samples.
  
  
  CONCLUSIONS
  A simple method for quantitative and qualitative assessment of the RNA from LCM samples is established, which can also be applied to assessment of DNA or proteins in LCM samples.
authors.affiliation: Institute of Neurobiology, Key Laboratory of Environment and Genes Related to Diseases of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. tianyf18@mail.xjtu.edu.cn
authors.forename: Ying-fang
authors.initials: YF
authors.lastname: Tian
authors.forename: Zhao-ming
authors.initials: ZM
authors.lastname: Wei
authors.forename: Xin-lin
authors.initials: XL
authors.lastname: Chen
authors.forename: Fen
authors.initials: F
authors.lastname: Qiu
authors.forename: Xin-li
authors.initials: XL
authors.lastname: Xiao
authors.forename: Qian-yan
authors.initials: QY
authors.lastname: Kang
authors.forename: Bo-feng
authors.initials: BF
authors.lastname: Zhu
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Tian
authors.forename: Jun-feng
authors.initials: JF
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002196:Capillaries / Q000473:pathology; D002540:Cerebral Cortex / Q000098:blood supply / Q000473:pathology*; D007834:Lasers; D008297:Male; D042282:Microdissection / Q000379:methods*; D009474:Neurons / Q000473:pathology; D012313:RNA / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D012313:RNA