Analysis of mixtures of purines and pyrimidines by first- and second-derivative ultraviolet spectrometry.
Zero-order, first-derivative and second-derivative ultraviolet absorption spectra of a series of purines, pyrimidines and their binary mixtures in aqueous solution have been recorded at 298 K. It is shown that second-derivative spectra can be used for the identification of eight mixtures of purines and pyrimidines. Several graphical procedures are tested for evaluating derivative spectra in quantitative measurements of single compounds and mixtures. Linear log-log calibration plots are obtained with correlation coefficients generally larger than 0.99. Second-derivative spectra appear to provide a precise and simple method for determination of purines and pyrimidines, at concentrations ranging between 5 x 10(-6) and 5 x 10(-4)M.
Aaron
J J
JJ
Institut de Topologie et de Dynamique des Systèmes, associé au C.N.R.S., Université Paris 7, 1, rue Guy de la Brosse, 75005 Paris, France.
Gaye
M D
MD