Preparation and characterization of perhydroxyl-cucurbit[6]uril bonded silica stationary phase for hydrophilic-interaction chromatography.
In this paper, perhydroxyl-cucurbit[6]uril ((HO)(12)CB[6]) was, for the first time, grafted to silica gel as a hydrophilic-interaction chromatographic stationary phase. Several alkaloids were used as probes to investigate the retention mechanism on the new stationary phase. The effect of mobile phase variables such as acetonitrile content, ionic strength and pH on their chromatographic behavior was investigated. The results indicate that the stationary phase behaves as a hydrophilic-interaction chromatographic packing. Finally, several alkaloids were separated on the stationary phase.
Liu
Si-Min
SM
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Xu
Li
L
Wu
Cheng-Tai
CT
Feng
Yu-Qi
YQ