title: Overall alteration of circadian clock gene expression in the chestnut cold response.
abstract: Cold acclimation in woody plants may have special features compared to similar processes in herbaceous plants. Recent studies have shown that circadian clock behavior in the chestnut tree (Castanea sativa) is disrupted by cold temperatures and that the primary oscillator feedback loop is not functional at 4 degrees C or in winter. In these conditions, CsTOC1 and CsLHY genes are constantly expressed. Here, we show that this alteration also affects CsPRR5, CsPRR7 and CsPRR9. These genes are homologous to the corresponding Arabidopsis PSEUDO-RESPONSE REGULATOR genes, which are also components of the circadian oscillator feedback network. The practically constant presence of mRNAs of the 5 chestnut genes at low temperature reveals an unknown aspect of clock regulation and suggests a mechanism regulating the transcription of oscillator genes as a whole.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Departamento de Biotecnología, Universidad Politécnica de Madrid, E. T. S. Ingenieros de Montes, Madrid, Spain.
authors.forename: Cristian
authors.initials: C
authors.lastname: Ibañez
authors.forename: Alberto
authors.initials: A
authors.lastname: Ramos
authors.forename: Paloma
authors.initials: P
authors.lastname: Acebo
authors.forename: Angela
authors.initials: A
authors.lastname: Contreras
authors.forename: Rosa
authors.initials: R
authors.lastname: Casado
authors.forename: Isabel
authors.initials: I
authors.lastname: Allona
authors.forename: Cipriano
authors.initials: C
authors.lastname: Aragoncillo
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D056926:CLOCK Proteins; D002940:Circadian Rhythm / Q000235:genetics; D003080:Cold Temperature; D029962:Fagaceae / Q000235:genetics* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000502:physiology; D010802:Phylogeny; D012097:Repressor Proteins; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; C523307:PRR5 protein, Arabidopsis; C523308:PRR7 protein, Arabidopsis; C514751:PRR9 protein, Arabidopsis; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D056926:CLOCK Proteins