title: New strategy for quantifying biological zinc by a modified zinpyr fluorescence sensor.
abstract: Substitution of one pyridine by pyrazine in each DPA appendage of ZP1 leads to a new zinc sensor, ZPP1, with a modified background fluorescence and zinc affinity. ZPP1 exhibits a two-step zinc response during fluorescence titrations, which leads to a new method for zinc quantification. The ability of ZPP1 to image and quantify zinc was demonstrated in pancreatic Min6 cells.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors.forename: Xiao-an
authors.initials: XA
authors.lastname: Zhang
authors.forename: Dugan
authors.initials: D
authors.lastname: Hayes
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Smith
authors.forename: Simone
authors.initials: S
authors.lastname: Friedle
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Lippard
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D005452:Fluoresceins / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000032:analysis* / Q000737:chemistry*
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D015032:Zinc; C501987:zinpyr-1