Metagenomic diagnosis of bacterial infections.
To test the ability of high-throughput DNA sequencing to detect bacterial pathogens, we used it on DNA from a patient's feces during and after diarrheal illness. Sequences showing best matches for Campylobacter jejuni were detected only in the illness sample. Various bacteria may be detectable with this metagenomic approach.
Nakamura
Shota
S
International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
Maeda
Norihiro
N
Miron
Ionut Mihai
IM
Yoh
Myonsun
M
Izutsu
Kaori
K
Kataoka
Chidoh
C
Honda
Takeshi
T
Yasunaga
Teruo
T
Nakaya
Takaaki
T
Kawai
Jun
J
Hayashizaki
Yoshihide
Y
Horii
Toshihiro
T
Iida
Tetsuya
T
D000328:Adult; D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods; D002169:Campylobacter Infections / Q000150:complications / Q000175:diagnosis; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D003937:Diagnosis, Differential; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic