Fluorimetric properties of a 2-hydroxypropyl-beta-cyclodextrin: 9-methyl-benzo[a]phenothiazine inclusion complex in aqueous media. Analytical usefulness.
The formation of an inclusion complex between 9-methyl-12H-benzo[a]phenothiazine (MeBPHT) and 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) was investigated in aqueous medium. A 12-fold fluorescence emission intensity enhancement was found for the complexed relative to the free analyte. MeBPHT forms a 1:1 stoichiometry complex with HP-beta-CD. A formation constant of 460 (+/-100) M(-1) was calculated using the Benesi-Hildebrand method and fluorimetric data. The limit of detection was 7 ng ml(-1) for MeBPHT in the presence of HP-beta-CD instead of 60 ng ml(-1) in the absence of HP-beta-CD.
Maafi
M
M
Institut de Topologie et de Dynamique des Systèmes de l'Université de Paris 7, Denis Diderot, associé au CNRS, URA-34, 1, rue Guy de la Brosse, 75005 Paris, France.
Mahedero
M C
MC
Aaron
J J
JJ