title: Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts.
abstract: One of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin-derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 (P<0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15 microM) induce cell cycle arrest at the G0/G1 phase.
authors.affiliation: Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Casiano H
authors.initials: CH
authors.lastname: Choresca
authors.forename: Ok Jae
authors.initials: OJ
authors.lastname: Koo
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Oh
authors.forename: So Gun
authors.initials: SG
authors.lastname: Hong
authors.forename: Dennis K
authors.initials: DK
authors.lastname: Gomez
authors.forename: Ji Hyung
authors.initials: JH
authors.lastname: Kim
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D018929:Cell Culture Techniques; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D016193:G1 Phase; D006054:Goldfish; D011687:Purines / Q000378:metabolism / Q000494:pharmacology; D016192:Resting Phase, Cell Cycle; D000077546:Roscovitine; D013217:Starvation
chemical_list: D011687:Purines; D000077546:Roscovitine