title: Flow-injection fluorimetric determination of mercury(II) with calcein.
abstract: A flow-injection spectrofluorimetric method for the determination of Hg(II) is described. The method is based on the complex that is formed between Hg(II) and calcein at pH 11-12. This reagent was not used before for determining Hg(II). The excitation wavelength is 324 nm and the emission wavelength is 522 nm. The calibration graph shows a linear range between 7.7 and 128 microg l(-1) (relative standard deviation 2.0%), the sampling rate was 90 h(-1). The effect of several interferents has been examined. Trace mercury in natural waters were determined satisfactorily using a modified manifold, which has an EDTA stream in order to minimize the calcium and magnesium interference.
authors.affiliation: FIA Laboratory, Department of Chemistry and Engineering Chemistry, Universidad Nacional del Sur. Av. Alem, Bahia Blanca, Argentina.
authors.forename: A G
authors.initials: AG
authors.lastname: Lista
authors.forename: M E
authors.initials: ME
authors.lastname: Palomeque
authors.forename: B S
authors.initials: BS
authors.lastname: Fernández Band