Application of amperometric sol-gel biosensor to flow injection determination of glucose.
A glucose biosensor with enzyme immobilised by sol-gel technology was constructed and evaluated. The glucose biosensor reported is based on encapsulated GOX within a sol-gel glass, prepared with 3-aminopropyltriethoxy silane, 2-(3,4-epoxycyclohexyl)-ethyltrimetoxy silane and HCl. A flow system incorporating the amperometric biosensor constructed was developed for the determination of glucose in the 1x10(-4)-5x10(-3) moll(-1) range with a precision of 1.5%. The results obtained for the analysis of electrolytic solution for iv administration and human serum samples showed good agreement between the proposed method and the reference procedure, with relative error <5%.
Couto
Cristina M C M
CM
CEQUP/Departamento de Quimica-Fisica, Faculdade de Farmácia (UP), Rua Anibal Cunha, 164-4050 Porto, Portugal; Instituto Superior de Ciências de Saúde, Norte, Rua Central da Gandra, 1317-4580 Gandra, Paredes, Portugal.
Araújo
Alberto N
AN
Montenegro
M Conceição B S M
MC
Rohwedder
Jarbas
J
Raimundo
Ivo
I
Pasquini
Célio
C