title: Polarography and artificial neural network for the simultaneous determination of nalidixic acid and its main metabolite (7-hydroxymethylnalidixic acid).
abstract: Nalidixic acid (NA) and its main metabolite, 7-hydroxymethylnalidixic acid (OH-NA), are simultaneously determined by applying artificial neural networks (ANNs), to their square wave voltammetric signals. The scores of a PCR model, built with the voltammetric data of a set of standard samples, recorded between -0.70 and -1.0V, are used as training set for the net for each compound. The trained nets (ANNs) are used for the simultaneous determination of NA and OH-NA in urine. The recovery values are comprised between 91 and 109% for NA and between 82 and 112% for OH-NA, being these results better than the results obtained by application of partial least squares (PLS) multivariate calibration.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Extremadura, Avda. Elvas s/n, 06071 Badajoz, Spain.
authors.forename: Agustina
authors.initials: A
authors.lastname: Guiberteau
authors.forename: Teresa Galeano
authors.initials: TG
authors.lastname: Díaz
authors.forename: María I
authors.initials: MI
authors.lastname: Rodríguez Cáceres
authors.forename: Juan M
authors.initials: JM
authors.lastname: Ortiz Burguillos
authors.forename: Isabel Durán
authors.initials: ID
authors.lastname: Merás
authors.forename: Francisco Salinas
authors.initials: FS
authors.lastname: López