title: Immunohistolocalization and gene expression of secretory carbonic anhydrase isoenzyme CA-VI in canine nasal cavity.
abstract: Immunolocalization of the secretory form of carbonic anhydrase isoenzyme, CA-VI were studied using a specific canine CA-VI antiserum, and CA-VI mRNA signals were also investigated using the reverse-transcriptase polymerase chain reaction (RT-PCR) in canine nasal mucosal epithelia and glands. Immunoreactivity to CA-VI was positive throughout the mucosal epithelial cells and in the cytoplasm of serous acinar and ductal epithelial cells of the nasal mucosa and glands, including the vestibule of the nose, but the mucous acinar cells of the glands were immunonegative. We detected CA-VI gene transcripts in the same regions as the CA-VI immunoreactivity. The physiological roles of CA-VI in the nasal mucosal epithelium and glands might maintain bicarbonate levels in nasal secretions and protect the mucosa against acid.
authors.affiliation: Department of Anatomy, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Sugiura
authors.forename: Nobutsune
authors.initials: N
authors.lastname: Ichihara
authors.forename: Toshiho
authors.initials: T
authors.lastname: Nishita
authors.forename: Masaru
authors.initials: M
authors.lastname: Murakami
authors.forename: Hajime
authors.initials: H
authors.lastname: Amasaki
authors.forename: Masao
authors.initials: M
authors.lastname: Asari
mesh_terms: D000818:Animals; D002256:Carbonic Anhydrases / Q000096:biosynthesis* / Q000235:genetics; D004285:Dogs / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry / Q000662:veterinary; D009296:Nasal Cavity / Q000201:enzymology*; D009297:Nasal Mucosa / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D012333:RNA, Messenger; D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI