Immunochemical composition of cryoglobulins generated in stroke.
BACKGROUND
Cryoglobulins are abnormal immune complexes where both the antigens and the antibodies are immunoglobulins. The ability of cryoglobulins to bind C-reactive protein and low density lipoproteins, activate complement, and stimulate production of tumor necrosis factor-alpha generates interest in studying cryoglobulins in ischemic stroke.


METHODS
We determined blood levels of cryoglobulins in patients with ischemic stroke at different time points of stroke onset and identified the composition of cryoglobulins isolated from the blood on the first day of stroke onset.


RESULTS
On days 1-14, significantly elevated levels of cryoglobulins were detected with the maximum level on day 3.


CONCLUSIONS
Determination of immunoglobulin (Ig) content of cryoglobulins revealed the presence of a mixture of polyclonal IgG, IgA, and IgM, C3 complement protein and its activation split products, C1q complement protein, pathogenic lipoprotein-X, and beta-lipoprotein.


CONCLUSIONS
We suggest that cryoglobulins are involved in post-ischemic inflammatory response through activation of the complement cascade and cytokines production.
Manukyan
Levon
L
Institute of Molecular Biology, National Academy of Sciences of Armenia, 7 Hasratyan St., Yerevan 0014, Armenia.
Boyajyan
Anna
A
Arakelyan
Arsen
A
Ayvazyan
Violetta
V
Arakelova
Elina
E
Sim
Robert
R
Grigoryan
George
G
D000368:Aged; D000936:Antigen-Antibody Complex / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D015922:Complement C1q / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D003176:Complement C3 / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D003450:Cryoglobulins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007120:Immunochemistry; D008073:Lipoprotein-X / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015427:Reperfusion Injury / Q000276:immunology / Q000378:metabolism; D020521:Stroke / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors
D000936:Antigen-Antibody Complex; D003176:Complement C3; D003450:Cryoglobulins; D008073:Lipoprotein-X; D008077:Lipoproteins, LDL; D015922:Complement C1q