A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on the immune system of marine mammals.
The cell lines chosen have demonstrated a positive response in terms of cell proliferation and associated modifications in proteins content, evaluated through DNA and proteins synthesis, at environmentally relevant dose of dioxins, brought by a typical environmental PCDD/F mixture. The response is time and species dependent. After completion of the identification of proteins affected by the intoxication, we will identify a set of specific proteins whose expression is correlated to the dioxin dose and submit the cell culture to the treatment with a single chemical as well as with mixtures. We hope that this will allow us to construct and validate a set of protein biomarkers of exposure to pollutants that will show a predictive aspect for unknown chemicals. The quantitative analysis of the set of biomarkers can then be a more specific bioassay and an alternative to physico-chemical or other already established bioanalytical methods for screening purposes.
Brenez
Cécile
C
Mass Spectrometry Laboratory, Faculty of Sciences, University of Liege, Allee De La Chimie, B-6C, Sart-Timan, B-4000 Liege, Belgium.
Gerkens
Pascal
P
Mazzucchelli
Gabriel
G
Jauniaux
Thierry
T
Eppe
Gauthier
G
Pauw
Edwin De
ED
Pauw-Gillet
Marie-Claire De
MC