Bioassay of nystatin: measurement of Mg2+ efflux by atomic-absorption spectroscopy.
A rapid bioassay for the polyene antibiotic nystatin, based on the leakage of Mg(2+) from sensitive cells of Saccharomyces cerevisiae, is described. The assay employs atomic-absorption spectrophotometry to measure the Mg(2+) leaked. It compares favourably with the classical method of diffusion on an agar-plate, in terms of speed, reproducibility and convenience.
Beezer
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Chemistry Department, Chelsea College, University of London, Manresa Road, London SW3, England.
Sharma
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