title: Routine phenotypic identification of bacterial species of the family Pasteurellaceae isolated from animals.
abstract: Pasteurellaceae are bacteria with an important role as primary or opportunistic, mainly respiratory, pathogens in domestic and wild animals. Some species of Pasteurellaceae cause severe diseases with high economic losses in commercial animal husbandry and are of great diagnostic concern. Because of new data on the phylogeny of Pasteurellaceae, their taxonomy has recently been revised profoundly, thus requiring an improved phenotypic differentiation procedure to identify the individual species of this family. A new and simplified procedure to identify species of Actinobacillus, Avibacterium, Gallibacterium, Haemophilus, Mannheimia, Nicoletella, and Pasteurella, which are most commonly isolated from clinical samples of diseased animals in veterinary diagnostic laboratories, is presented in the current study. The identification procedure was evaluated with 40 type and reference strains and with 267 strains from routine diagnostic analysis of various animal species, including 28 different bacterial species. Type, reference, and field strains were analyzed by 16S ribosomal RNA (rrs) and rpoB gene sequencing for unambiguous species determination as a basis to evaluate the phenotypic differentiation schema. Primary phenotypic differentiation is based on beta-nicotinamide adenine dinucleotide (beta-NAD) dependence and hemolysis, which are readily determined on the isolation medium. The procedure divides the 28 species into 4 groups for which particular biochemical reactions were chosen to identify the bacterial species. The phenotypic identification procedure allowed researchers to determine the species of 240 out of 267 field strains. The procedure is an easy and cost-effective system for the rapid identification of species of the Pasteurellaceae family isolated from clinical specimens of animals.
authors.affiliation: Institute of Veterinary Bacteriology, Universität Bern, Laenggasstrasse 122, Bern, Switzerland.
authors.forename: Florence
authors.initials: F
authors.lastname: Dousse
authors.forename: Andreas
authors.initials: A
authors.lastname: Thomann
authors.forename: Isabelle
authors.initials: I
authors.lastname: Brodard
authors.forename: Bozena M
authors.initials: BM
authors.lastname: Korczak
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Schlatter
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhnert
authors.forename: Raymond
authors.initials: R
authors.lastname: Miserez
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
mesh_terms: D000188:Actinobacillus / Q000235:genetics / Q000302:isolation & purification; D000333:Aeromonas / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D000829:Animals, Domestic; D016950:Bordetella bronchiseptica / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D031121:Mannheimia / Q000235:genetics / Q000302:isolation & purification; D015661:Pasteurellaceae / Q000235:genetics* / Q000302:isolation & purification; D016871:Pasteurellaceae Infections / Q000175:diagnosis* / Q000662:veterinary; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification