[Dynamic determination of sinomenine in skin by microdialysis].
OBJECTIVE
To explore the feasibility and advantages of using microdialysis as sampling method for dynamic determination of sinomenine in topical skin.


METHODS
In this study, sinomenine was administered to the rats by transdermal drug delivery system and celiac injection. With microdialysis technique for sampling, the concentration of sinomenine in dialysate was determined by high performance liquid chromatography (HPLC).


RESULTS
Under existing determination condition, topical drug concentration in the skin of rats was hard to be detected after sinomenine administered to the skin, but it could be detected after celiac injection.


CONCLUSIONS
Microdialysis sampling method can be used to determine topical drug concentration in the skin dynamically, and this method is better than traditional methods obviously.
Ling
Jia-Jun
JJ
College of Chinese Drug, Guangzhou University of TCM, Guangzhou 510006, China. ljj@gahtcm.edu.cn
Xie
Bo
B
Wang
Yan
Y
Zhou
Li-Ling
LL
D000279:Administration, Cutaneous; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005240:Feasibility Studies; D007274:Injections, Intraperitoneal; D008297:Male; D029583:Menispermaceae / Q000737:chemistry*; D017551:Microdialysis / Q000379:methods*; D009019:Morphinans / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism*
D009019:Morphinans; C009271:sinomenine