title: Simultaneous determination of jujuboside A, B and betulinic acid in semen Ziziphi spinosae by high performance liquid chromatography-evaporative light scattering detection.
abstract: A reverse phase high performance liquid chromatographic (HPLC) method with evaporative light scattering detection (ELSD) was developed for simultaneous determination of jujuboside A, B and betulinic acid in semen Ziziphi spinosae. The analysis was performed by gradient elution, using an aqueous mobile phase (containing 0.1% acetic acid) modified by acetonitrile. The evaporator tube temperature of ELSD was set at 45 degrees C, and with the nebulizing gas flow-rate of 1.8l/min. The method was validated for accuracy, reproducibility, precision and limits of detection and quantification. Quantification of the three active compounds in semen Ziziphi spinosae from different locations was performed by this method, which provides a new tool for quality assessment of semen Ziziphi spinosae.
authors.affiliation: Department of Pharmaceutical Engineering, Tianjin University of Commerce, Tianjin 300134, China.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Zhang
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Junbo
authors.initials: J
authors.lastname: Xie
mesh_terms: D015415:Biomarkers / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008027:Light; D008516:Medicine, Chinese Traditional / Q000379:methods*; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes; D012015:Reference Standards; D015203:Reproducibility of Results; D012503:Saponins / Q000737:chemistry*; D012542:Scattering, Radiation; D013696:Temperature; D014315:Triterpenes / Q000737:chemistry*; D014835:Volatilization; D031957:Ziziphus / Q000737:chemistry*; D000094062:Betulinic Acid
chemical_list: D015415:Biomarkers; D053978:Pentacyclic Triterpenes; D012503:Saponins; D014315:Triterpenes; C540008:jujuboside B; C087808:jujuboside A; D000094062:Betulinic Acid