Possibilities for separation and simultaneous determination of N-unsubstituted- and N-substituted nitroimidazoles and criteria for their identification.
The N-unsubstituted nitroimidazoles have an imino hydrogen atom in contrast to the N-substituted derivatives, and react with hydroxide to give the yellow nitroimidazole anions. The difficulty of reducing the nitro-group in these anions, together with the shift of the absorption maximum to longer wavelengths makes it possible to analyse polarographically or spectrophotometrically a mixture of N-unsubstituted nitroimidazole with N-substituted derivatives. The presence of the N-unsubstituted nitroimidazole and the N-substituted derivatives is first established by chromatography. Other criteria are also proposed for distinguishing between these substances.
Dumanović
D
D
Research Laboratory "Galenika", Zemun, Yugoslavia.
Perkucin
S
S
Volke
J
J