Development and validation of reversed-phase column high-performance liquid chromatographic and first-derivative UV spectrophotometric methods for estimation of voriconazole in oral suspension powder.
This research paper describes validated reversed-phase high-performance column liquid chromatographic (RP-HPLC) and first-derivative UV spectrophotometric methods for the estimation of voriconazole (VOR) in oral suspension powder. The RP-HPLC separation was achieved on Phenomenex C18 column (250 x 4.6 mm id, 5 microm particle size) using water-acetonitrile (40 + 60, v/v; pH adjusted to 4.5 +/- 0.02 with acetic acid) as the mobile phase at a flow rate of 1.4 mL/min and ambient temperature. Quantification was achieved with photodiode array detection at 255 nm over the concentration range of 0.1-1 microg/mL with mean recovery of 99.49 +/- 0.83% for VOR by the RP-HPLC method. Quantification was achieved with UV detection at 266 nm over the concentration range of 8-20 microg/mL with mean recovery of 99.74 +/- 0.664% for VOR by the first-derivative UV spectrophotometric method. These methods are simple, precise, and sensitive, and they are applicable for the determination of VOR in oral suspension powder.
Prajapati
Arun M
AM
Ganpat University, Shree S.K. Patel College of Pharmaceutical Education and Research, Department of Pharmaceutical Chemistry, Ganpat Vidyanagar, Kherva, Mehsana, Gujarat, India. arun_time@yahoo.co.in
Patel
Satish A
SA
Patel
Natvarlal J
NJ
Patel
Dipti B
DB
Patel
Sejal K
SK
D000284:Administration, Oral; D000935:Antifungal Agents / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D011743:Pyrimidines / Q000032:analysis*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D013535:Suspensions; D014230:Triazoles / Q000032:analysis*; D065819:Voriconazole
D000935:Antifungal Agents; D011743:Pyrimidines; D013535:Suspensions; D014230:Triazoles; D065819:Voriconazole