Preparation and anti-tumor efficiency evaluation of doxorubicin-loaded bacterial magnetosomes: magnetic nanoparticles as drug carriers isolated from Magnetospirillum gryphiswaldense.
Bacterial magnetosomes (BMs) are commonly used as vehicles for certain enzymes, nucleic acids and antibodies, although they have never been considered drug carriers. To evaluate the clinical potential of BMs extracted from Magnetospirillum gryphiswaldense in cancer therapy, doxorubicin (DOX) was loaded onto the purified BMs at a ratio of 0.87 +/- 0.08 mg/mg using glutaraldehyde. The DOX-coupled BMs (DBMs) and BMs exhibited uniform sizes and morphology evaluated by TEM. The diameters of DBMs and BMs obtained by AFM were 71.02 +/- 6.73 and 34.93 +/- 8.24 nm, respectively. The DBMs released DOX slowly into serum and maintained at least 80% stability following 48 h of incubation. In vitro cytotoxic tests showed that the DBMs were cytotoxic to HL60 and EMT-6 cells, manifested as inhibition of cell proliferation and suppression in c-myc expression, consistent with DOX. These observations depicted in vitro antitumor property of DBMs similar to DOX. The approach of coupling DOX to magnetosomes may have clinical potential in anti-tumor drug delivery.
Sun
Jian-Bo
JB
State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing, PR China.
Duan
Jin-Hong
JH
Dai
Shun-Ling
SL
Ren
Jun
J
Guo
Lin
L
Jiang
Wei
W
Li
Ying
Y
D000970:Antineoplastic Agents / Q000378:metabolism* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004317:Doxorubicin / Q000378:metabolism* / Q000494:pharmacology*; D004337:Drug Carriers / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008280:Magnetics; D042201:Magnetospirillum / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure
D000970:Antineoplastic Agents; D004337:Drug Carriers; D004317:Doxorubicin