Isolation and characterization of Argonaute 2: a key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus.
Argonaute 2 gene plays a pivotal role in RNAi in many species. Herein is the first report of the cloning and characterization of Argonaute 2 gene in fish. The full-length cDNA of Gobiocypris rarus Argonaute 2 (GrAgo2) consisted of 3073 nucleotides encoding 869 amino acid residues with a calculated molecular weight of 98.499 kDa and an estimated isoelectric point of 9.18. Analysis of the deduced amino acid sequence showed the presence of two signature domains, PAZ and Piwi. RT-PCR analysis indicated that GrAgo2 mRNA expression could be detected in widespread tissues. After infection with grass carp reovirus, GrAgo2 expression was up-regulated from 12 h post-injection (p < 0.05) and returned to control levels at 48 h post-injection (p > 0.05). These data imply that GrAgo2 is involved in antiviral defense in rare minnow.
Su
Jianguo
J
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China.
Zhu
Zuoyan
Z
Wang
Yaping
Y
Jang
Songhun
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003530:Cyprinidae / Q000254:growth & development* / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010802:Phylogeny; D034622:RNA Interference; D034743:RNA-Induced Silencing Complex / Q000235:genetics*; D012087:Reoviridae / Q000502:physiology; D012088:Reoviridae Infections / Q000662:veterinary*; D016415:Sequence Alignment; D013997:Time Factors
D034743:RNA-Induced Silencing Complex