Quantitative determination of sulfonamide in meat by liquid chromatography-electrospray-mass spectrometry.
This paper describes a newly developed liquid chromatography-electrospray-mass spectrometry (LC-ES-MS) method for the quantitative determination of nine commonly used sulfonamides (sulfadiazine, sulfapyridine, sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfisoxazole, sulfadimethoxine, sulfaquinoaline and sulfaphenazole) in meat. [M+H](+) and [M+Na](+) were the two major ions detected in positive ion mode. Selective ion monitoring was employed for quantitative determination. Satisfactory linearity, 0.1-10 mugml(-1), of each compound was obtained. Blank meat samples were fortified at levels between 50 and 500 mugkg(-1). [Phenyl-(13)C6]sulfamethazine was used as internal standard. Sulfonamides were isolated from meat with a solvent extraction procedure and then determined by LC-ES-MS. The limits of detection were below 10 mugkg(-1). The application of this newly developed method was demonstrated by analyzing various beef, pork and chicken samples from local markets.
Fuh
M R
MR
Department of Chemistry, Soochow University, P.O. Box 86-72, Taipei, Taiwan, ROC.
Chan
S A
SA