title: JunB is a repressor of MMP-9 transcription in depolarized rat brain neurons.
abstract: Matrix Metalloproteinase-9 (MMP-9) is an extracellularly operating enzyme involved in the synaptic plasticity, hippocampal-dependent long term memory and neurodegeneration. Previous studies have shown its upregulation following seizure-evoking stimuli. Herein, we show that in the rat brain, MMP-9 mRNA expression in response to pentylenetetrazole-evoked neuronal depolarization is transient. Furthermore, we demonstrate that in the rat hippocampus neuronal activation strongly induces JunB expression, simultaneously leading to an accumulation of JunB/FosB complexes onto the -88/-80 bp site of the rat MMP-9 gene promoter in vivo. Surprisingly, manipulations with JunB expression levels in activated neurons revealed its moderate repressive action onto MMP-9 gene expression. Therefore, our study documents the active repressive influence of AP-1 onto MMP-9 transcriptional regulation by the engagement of JunB.
authors.affiliation: Department of Molecular and Cellular Neurobiology, Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland. mrylski@nencki.gov.pl
authors.forename: Marcin
authors.initials: M
authors.lastname: Rylski
authors.forename: Renata
authors.initials: R
authors.lastname: Amborska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zybura
authors.forename: Piotr
authors.initials: P
authors.lastname: Michaluk
authors.forename: Beata
authors.initials: B
authors.lastname: Bielinska
authors.forename: Filip A
authors.initials: FA
authors.lastname: Konopacki
authors.forename: Grzegorz M
authors.initials: GM
authors.lastname: Wilczynski
authors.forename: Leszek
authors.initials: L
authors.lastname: Kaczmarek
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003292:Convulsants / Q000494:pharmacology; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D008564:Membrane Potentials / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010433:Pentylenetetrazole / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
chemical_list: D003292:Convulsants; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D020780:Matrix Metalloproteinase 9; D010433:Pentylenetetrazole