title: Undesired effects of a combinatorial treatment for spinal cord injury--transplantation of olfactory ensheathing cells and BDNF infusion to the red nucleus.
abstract: Transplantations of olfactory ensheathing cells (OECs) have been reported to promote axonal regeneration and functional recovery after spinal cord injury, but have demonstrated limited growth promotion of rat rubrospinal axons after a cervical dorsolateral funiculus crush. Rubrospinal neurons undergo massive atrophy after cervical axotomy and show only transient expression of regeneration-associated genes. Cell body treatment with brain-derived neurotrophic factor (BDNF) prevents this atrophy, stimulates regeneration-associated gene expression and promotes regeneration of rubrospinal axons into peripheral nerve transplants. Here, we hypothesized that the failure of rubrospinal axons to regenerate through a bridge of OEC transplants was due to this weak intrinsic cell body response. Hence, we combined BDNF treatment of rubrospinal neurons with transplantation of highly enriched OECs derived from the nasal mucosa and assessed axonal regeneration as well as behavioral changes after a cervical dorsolateral funiculus crush. Each treatment alone as well as their combination prevented the dieback of the rubrospinal axons, but none of them promoted rubrospinal regeneration beyond the lesion/transplantation site. Motor performance in a food-pellet reaching test and forelimb usage during vertical exploration (cylinder test) were more impaired after combining transplantation of OECs with BDNF treatment. This impaired motor performance correlated with lowered sensory thresholds in animals receiving the combinatorial therapy - which were not seen with each treatment alone. Only this combinatorial treatment group showed enhanced sprouting of calcitonin gene-related peptide-positive axons rostral to the lesion site. Hence, some combinatorial treatments, such as OECs with BDNF, may have undesired effects in the injured spinal cord.
authors.affiliation: ICORD (International Collaboration On Repair Discoveries), Department of Zoology, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Frederic
authors.initials: F
authors.lastname: Bretzner
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Erin
authors.initials: E
authors.lastname: Currie
authors.forename: A Jane
authors.initials: AJ
authors.lastname: Roskams
authors.forename: Wolfram
authors.initials: W
authors.lastname: Tetzlaff
mesh_terms: D000818:Animals; D019771:Axotomy / Q000009:adverse effects; D016380:Brain Tissue Transplantation / Q000009:adverse effects*; D019208:Brain-Derived Neurotrophic Factor / Q000009:adverse effects*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004525:Efferent Pathways / Q000187:drug effects / Q000293:injuries / Q000503:physiopathology; D020439:Growth Cones / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009069:Movement Disorders / Q000188:drug therapy / Q000503:physiopathology / Q000601:surgery; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012012:Red Nucleus / Q000187:drug effects* / Q000503:physiopathology; D012183:Retrograde Degeneration / Q000188:drug therapy / Q000503:physiopathology / Q000517:prevention & control; D012684:Sensory Thresholds / Q000502:physiology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000503:physiopathology / Q000601:surgery*
chemical_list: D019208:Brain-Derived Neurotrophic Factor