title: Seminal plasma proteins of Atlantic halibut (Hippoglossus hippoglossus L.).
abstract: Protein content and properties in the seminal plasma of Atlantic halibut (Hippoglossus hippoglossus) were assayed using spectrophotometric and electrophoretic methods. The protein concentration ranged from 6.4 +/- 3.1 to 19.4 +/- 3.4 mg ml(-1) and anti-proteolytic activity from 585.2 +/- 104.6 to 2912.4 +/- 367.4 U l(-l). A high correlation between anti-proteolytic activity and protein concentration (r = 0.95), and between sperm concentration and osmolality was found (r = 0.92). There was a significant decrease in anti-proteolytic activity from the first to the second sampling, but not in protein concentration. Anti-proteolytic activity and protein concentration were significantly affected by variations in individual males. Electrophoresis revealed four anti-proteolytic bands and individual differences in bands of proteolytic activity, which were subsequently characterized as metalloproteases and serine proteases.
authors.affiliation: Department of Fisheries and Natural Sciences, Bodø University College, 8049 Bodo, Norway.
authors.forename: Maren
authors.initials: M
authors.lastname: Mommens
authors.forename: Mariola
authors.initials: M
authors.lastname: Wojtczak
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ciereszko
authors.forename: Igor
authors.initials: I
authors.lastname: Babiak
mesh_terms: D000818:Animals; D001550:Benzamidines / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005432:Flounder / Q000378:metabolism / Q000502:physiology*; D018093:Gelatinases / Q000378:metabolism; D008297:Male; D009994:Osmolar Concentration; D012661:Semen / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D029589:Seminal Plasma Proteins / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013076:Sperm Count
chemical_list: D001550:Benzamidines; D002614:Chelating Agents; D029589:Seminal Plasma Proteins; D015842:Serine Proteinase Inhibitors; D004492:Edetic Acid; D018093:Gelatinases; C032157:benzamidine; D002122:Calcium Chloride