title: Determination of nickel in the serum of occupationally exposed workers, by means of flame atomic-absorption spectroscopy.
abstract: A flame atomic-absorption spectroscopy method for determination of nickel in the serum of occupationally exposed subjects has been developed. Trichloroacetic add is utilized for precipitating proteins and freeing bound nickel; sodium diethyldithiocarbamate is used as complexing agent and isopropyl acetate as the solvent for extraction. The method is characterized by good accuracy, precision and sensitivity, over a concentration range up to about 20 ng/ml. Calcium, which is present in serum in great excess with respect to typical nickel concentrations, does not interfere in the determination of the latter.
authors.affiliation: Istituto Chimico G. Ciamician dell'Università, Scuola di Specializzazione in Chimica Analitica, Via Selmi 2, 40126 Bologna, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Ghe
authors.forename: M T
authors.initials: MT
authors.lastname: Lippolis
authors.forename: L
authors.initials: L
authors.lastname: Pastorelli