title: Decolorization of textile effluent by bitter gourd peroxidase immobilized on concanavalin A layered calcium alginate-starch beads.
abstract: Bitter gourd peroxidase immobilized on the surface of concanavalin A layered calcium alginate-starch beads was used for the successful and effective decolorization of textile industrial effluent. Effluent was recalcitrant to the action of bitter gourd peroxidase; however, in the presence of some redox mediators, it was successfully decolorized. Effluent decolorization was maximum (70%) in the presence of 1.0mM 1-hydroxybenzotriazole within 1h of incubation. However, immobilized bitter gourd peroxidase showed maximum decolorization at pH 5.0 and 40 degrees C. Immobilized bitter gourd peroxidase decolorized more than 90% effluent after 3h of incubation in a batch process. The two-reactor system, one reactor containing immobilized peroxidase and the other had activated silica, was quite effective in the decolorization of textile effluent. The system was capable of decolorizing 40% effluent even after 2 months of continuous operation. The absorption spectra of the untreated and treated effluent exhibited a marked difference in absorbance at various wavelengths. Immobilized peroxidase/1-hydroxybenzotriazole system could be employed for the treatment of a large volume of effluent in a continuous reactor.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India.
authors.forename: Mahreen
authors.initials: M
authors.lastname: Matto
authors.forename: Qayyum
authors.initials: Q
authors.lastname: Husain
mesh_terms: D004396:Coloring Agents / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism; D007220:Industrial Waste / Q000517:prevention & control; D029777:Momordica charantia / Q000201:enzymology; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism*; D013784:Textiles; D018508:Water Purification / Q000379:methods*
chemical_list: D004396:Coloring Agents; D004800:Enzymes, Immobilized; D007220:Industrial Waste; D009195:Peroxidase