title: Effect of bone marrow-derived mesenchymal stem cells on cardiovascular complications in diabetic rats.
abstract: BACKGROUND
  The purpose of this study was to investigate the effect of mesenchymal stem cells (MSCs) on cardiovascular complications of type 1 diabetes mellitus (DM) in rats.
  
  
  METHODS
  MSCs were derived from the bone marrow of male albino rats. The MSCs were characterized morphologically and by RT-PCR for CD29 expression. They were then infused into female rats which were made diabetic by IP injection of streptozotocin (STZ). The rats were divided into control, STZ, and STZ plus MSC groups. Serum insulin, glucose, and fibrinogen were estimated in all groups and the Y-chromosome gene sry was detected by PCR in pancreatic and cardiac tissues. Physiological cardiovascular functions (heart rate, systolic blood pressure) were assessed by a Langendorff apparatus.
  
  
  RESULTS
  Diabetic rats which received MSCs showed significantly lower serum glucose and increased serum insulin levels compared with the STZ group. Improvement of cardiovascular performance was also observed in the STZ/MSC group compared with the STZ group. The sry gene was detected by PCR in the pancreatic and cardiac tissues of the STZ/MSC group.
  
  
  CONCLUSIONS
  Rat bone marrow harbors cells that have the capacity to differentiate into functional insulin-producing cells capable of controlling blood glucose level in diabetic rats. This may provide a source of cell-based therapy for DM. Furthermore, MSC transplantation can improve cardiac function in DM.<
authors.affiliation: Unit of Biochemistry and Molecular Biology, Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt. talaatabdaziz@yahoo.co.uk
authors.forename: Mohamed Talaat
authors.initials: MT
authors.lastname: Abdel Aziz
authors.forename: Mohamed Farid
authors.initials: MF
authors.lastname: El-Asmar
authors.forename: Mohamed
authors.initials: M
authors.lastname: Haidara
authors.forename: Hazem Mahmoud
authors.initials: HM
authors.lastname: Atta
authors.forename: Nagwa Kamal
authors.initials: NK
authors.lastname: Roshdy
authors.forename: Laila Ahmed
authors.initials: LA
authors.lastname: Rashed
authors.forename: Dina
authors.initials: D
authors.lastname: Sabry
authors.forename: Mary Andraws
authors.initials: MA
authors.lastname: Youssef
authors.forename: Ahmed Talaat
authors.initials: AT
authors.lastname: Abdel Aziz
authors.forename: Manal
authors.initials: M
authors.lastname: Moustafa
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure; D001835:Body Weight; D001854:Bone Marrow Cells / Q000166:cytology*; D002318:Cardiovascular Diseases / Q000209:etiology* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D002454:Cell Differentiation; D002478:Cells, Cultured; D048909:Diabetes Complications / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D007328:Insulin / Q000097:blood; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats
chemical_list: D001786:Blood Glucose; D007328:Insulin; D019012:Integrin beta1