Dynamic contrast-enhanced magnetic resonance imaging of tumor interstitial fluid pressure.
OBJECTIVE
High interstitial fluid pressure (IFP) in the primary tumor has been shown to promote metastasis in melanoma xenografts and to predict for poor survival in cervical cancer patients. The potential usefulness of gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA)-based dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) for assessing tumor IFP noninvasively was investigated in the present study.


METHODS
A-07 and R-18 melanoma xenografts with and without necrotic regions were subjected to DCE-MRI and subsequent measurement of IFP. Tumor images of E.F (E is the initial extraction fraction of Gd-DTPA and F is blood perfusion) and lambda (lambda is proportional to extracellular volume fraction) were produced by Kety analysis of DCE-MRI series.


RESULTS
In tumors without necrosis, significant inverse correlations were found between E.F and IFP, both for A-07 and R-18 tumors, and between lambda and IFP for A-07 tumors. Significant correlations between E.F and IFP or between lambda and IFP could not be detected for tumors with necrotic regions.


CONCLUSIONS
DCE-MRI may be developed to be a useful noninvasive method for assessing IFP in tumors without necrosis. This possibility warrants further studies involving several physiologically different experimental tumor lines, as well as human tumors.
Gulliksrud
Kristine
K
Department of Radiation Biology, Institute for Cancer Research, Oslo, Norway.
Brurberg
Kjetil G
KG
Rofstad
Einar K
EK
D000818:Animals; D003287:Contrast Media / Q000493:pharmacokinetics; D045604:Extracellular Fluid; D005260:Female; D019786:Gadolinium DTPA / Q000493:pharmacokinetics; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009336:Necrosis; D009368:Neoplasm Transplantation; D011312:Pressure; D018709:Statistics, Nonparametric
D003287:Contrast Media; D019786:Gadolinium DTPA