Metabolism and excretion study of DW116, a new fluoroquinolone, in rats.
Metabolite identification and urinary and biliary excretion of the new fluoroquinolone antibacterial agent DW116 [1-(5-fluoro-2-pyridyl)-6-fluoro-7-(4-methyl-1-piperazinyl)-1,4-dihydro-4-oxoquinoline-3-carboxylic acid, hydrochloride] after oral administration have been studied in Sprague-Dawley rats. The excretion kinetics were monoexponential. Most of the drug was eliminated via the hepatic and renal routes. Mean renal clearance of DW116 was 73.4 ml/hr/kg and mean biliary clearance was 83.8 ml/hr/kg. The major metabolite excreted in the bile was identified as the glucuronide ester of the parent drug using base-hydrolysis of the conjugate metabolite followed by co-HPLC with standard compound,(19)F-NMR and LC-MS methods. The glucuronide conjugate was also found in urine. The mean urinary recoveries of free and total (free plus glucuronide ester) DW116 were 28.6+/-2.7% and 36.4+/-1.8% of the administered dose and the corresponding biliary recoveries were 14.4+/-5.5% and 37.0+/-7.6%, respectively.
Jung
B H
BH
Doping Control Center, Korea Institute of Science and Technology, Cheongryang, P.O. Box 131, 130-650, Seoul, Korea.
Park
Y H
YH
Park
J
J
Chung
B C
BC