Determination of the fatty-acid composition of soybean oil by high-pressure liquid chromatography.
A method for determining the fatty-acid composition of soybean oil by using high-pressure liquid chromatography (HPLC) is discussed and compared with the determination using gas chromatography. The oil is saponified and an aliquot is treated directly to form the p-bromophenacyl esters of the fatty acids, which are separated by gradient-elution HPLC. It is shown that glycerol does not interfere with the esterification, thus obviating the solvent extraction previously used to isolate fatty acids from biological samples.
Jaselskis
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B
Loyola University of Chicago, 6525 North Sheridan Road, Chicago, Illinois 60626, USA.
Stemm
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NL
Johnston
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WD