Validation of an enzyme-linked immunosorbent assay (ELISA) for the determination of 4-nonylphenol and octylphenol in surface water samples by LC-ESI-MS.
4-Nonylphenol (NP) and octylphenol (OP) were measured by direct ELISA in both laboratory-fortified and surface water samples collected monthly from 10 rivers. In this procedure, samples were concentrated by solid phase extraction (SPE) using Lichrolut RP-18 sorbent with good recoveries obtained for both LC-MS and ELISA, giving a low level of detection (LOD) at the range of low mugL(-1) and good reproducibility. Analysis of 40 surface water samples demonstrated that the ELISA may be a useful screening tool for the determination of the alkylphenols in surface water matrices. The concentration of NP and OP in surface waters ranged from 0.11 to 6.58mugL(-1). A good correlation of results obtained by ELISA and LC-MS within the concentration range of 0.08-6.86mugL(-1) was found in the river samples [R(2)=0.924, n=39]. The influence of various factors on assay determination was also discussed.
Céspedes
R
R
Department of Environmental Chemistry, IIQAB-CSIC, Barcelona, Spain.
Skryjová
K
K
Raková
M
M
Zeravik
J
J
Fránek
M
M
Lacorte
S
S
Barceló
D
D