title: Lymph-migrating, tissue-derived dendritic cells are minor constituents within steady-state lymph nodes.
abstract: Observations that dendritic cells (DCs) constitutively enter afferent lymphatic vessels in many organs and that DCs in some tissues, such as the lung, turnover rapidly in the steady state have led to the concept that a major fraction of lymph node DCs are derived from migratory DCs that enter the lymph node through upstream afferent lymphatic vessels. We used the lysozyme M-Cre reporter mouse strain to assess the relationship of lymph node and nonlymphoid organ DCs. Our findings challenge the idea that a substantial proportion of lymph node DCs derive from the upstream tissue during homeostasis. Instead, our analysis suggests that nonlymphoid organ DCs comprise a major population of DCs within lymph nodes only after introduction of an inflammatory stimulus.
authors.affiliation: Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors.forename: Claudia
authors.initials: C
authors.lastname: Jakubzick
authors.forename: Milena
authors.initials: M
authors.lastname: Bogunovic
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Bonito
authors.forename: Emma L
authors.initials: EL
authors.lastname: Kuan
authors.forename: Miriam
authors.initials: M
authors.lastname: Merad
authors.forename: Gwendalyn J
authors.initials: GJ
authors.lastname: Randolph
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006706:Homeostasis; D007249:Inflammation / Q000276:immunology*; D008168:Lung / Q000166:cytology / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D042601:Lymphatic Vessels / Q000166:cytology* / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009113:Muramidase / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D049452:Green Fluorescent Proteins; D009113:Muramidase; C472450:lysozyme M, mouse