title: Assessment of novel chemical strategies for covalent attachment of adhesive peptides to rough titanium surfaces: XPS analysis and biological evaluation.
abstract: Bioactive molecules have been proposed to promote beneficial interactions at bone-implant interfaces for enhancing integration. The main objective of this study was to develop novel methods to functionalize oxidized titanium surfaces by the covalent immobilization of bioactive peptides, through selective reaction involving single functional groups. In the first protocol, an aminoalkylsilane was covalently linked to the Ti oxide layer, followed by covalent binding of glutaric anhydride to the free NH(2) groups. The carboxylic group of glutaric anhydride was used to condense the free N-terminal group of the side-chain protected peptide sequence. Finally, the surface was treated with trifluoroacetic acid to deprotect side-chain groups. In the second protocol, the peptide was directly anchored to the Ti oxide surface via UV activation of an arylazide peptide analogue. X-ray photoelectron spectroscopy analyses confirmed that modifications induced onto surface composition were in agreement with the reactions performed. The peptide density of each biomimetic surface was determined on the basis of radiolabeling and XPS derived reaction yields. The in vitro cellular response of the biomimetic surfaces was evaluated using a primary human osteoblast cell model. Cell adhesion, proliferation, differentiation, and mineralization were examined at initial-, short-, and long-time periods. In was shown that the biomimetic surface obtained through photoprobe-marked analogue that combines an easily-performed modification provides a favorable surface for an enhanced cellular response.
authors.affiliation: Department of Chemical Process Engineering, University of Padova, via Marzolo 9, Padua, Italy. monica.dettin@unipd.it
authors.forename: Monica
authors.initials: M
authors.lastname: Dettin
authors.forename: Thushari
authors.initials: T
authors.lastname: Herath
authors.forename: Roberta
authors.initials: R
authors.lastname: Gambaretto
authors.forename: Giovanna
authors.initials: G
authors.lastname: Iucci
authors.forename: Chiara
authors.initials: C
authors.lastname: Battocchio
authors.forename: Andrea
authors.initials: A
authors.lastname: Bagno
authors.forename: Francesca
authors.initials: F
authors.lastname: Ghezzo
authors.forename: Carlo
authors.initials: C
authors.lastname: Di Bello
authors.forename: Giovanni
authors.initials: G
authors.lastname: Polzonetti
authors.forename: Lucy
authors.initials: L
authors.lastname: Di Silvio
mesh_terms: D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D010455:Peptides / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D010455:Peptides; D014025:Titanium