title: Construction of asymmetric cell-sized lipid vesicles from lipid-coated water-in-oil microdroplets.
abstract: We present a simple, rapid, and robust method for preparing asymmetric cell-sized lipid bilayer vesicles using water-in-oil (W/O) microdroplets transferred through an oil-water interface. The efficiency for producing cell-sized model membranes is elucidated in relation to the vesicular size and the weight of contained water-soluble molecules. We demonstrate the biological asymmetric nature and the formation of lipid raft microdomain structures using fluorescence microscopy.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hamada
authors.forename: Yoko
authors.initials: Y
authors.lastname: Miura
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Komatsu
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kishimoto
authors.forename: Mun'delanji
authors.initials: M
authors.lastname: Vestergaard
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takagi
mesh_terms: D008051:Lipid Bilayers; D008055:Lipids / Q000737:chemistry*; D009821:Oils / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008055:Lipids; D009821:Oils; D014867:Water