title: Electrochemiluminescent behaviors of alkaloids and tris(2,2'-bipyridine) ruthenium in organically modified silicate film.
abstract: Electrogenerated chemiluminescences (ECLs) of alkaloids, such as berberine, trigonelline, allantoin and betaine, were studied in an aqueous alkaline buffer solution (pH 9.5), based on tris(2,2'-bipyridine)ruthenium(II) [Ru(bpy)(3)(2+)] immobilized in organically modified silicates (ORMOSILs) film on a glassy carbon electrode (GCE). The immobilized Ru(bpy)(3)(2+) showed good electrochemical and photochemical activities. In a flow system, the eluted alkaloids were oxidized on the modified GCE, and reacted with immobilized Ru(bpy)(3)(2+) at the potential of +1.50V (versus Ag/AgCl). The luminescence with lambda(max) 610nm was caused by a reaction of electrolytically formed Ru(bpy)(3)(3+) with an oxidized amine group to generate Ru(bpy)(3)(2+*). The determination limit was 5x10(-6)molL(-1), 8x10(-6)molL(-1), 2.0x10(-5)molL(-1) and 5.0x10(-5)molL(-1) for berberine, trigonelline, allantoin and betaine at S/N 3, respectively. In addition, the factors affecting the determination of the four alkaloids were also studied.
authors.affiliation: Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Tao
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Yang
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhang
authors.forename: Munetaka
authors.initials: M
authors.lastname: Oyama
authors.forename: Xi
authors.initials: X
authors.lastname: Chen