title: Clinically relevant dose of zoledronic acid inhibits spontaneous lung metastasis in a murine osteosarcoma model.
abstract: Clinically obtainable concentrations of zoledronic acid (ZOL) inhibited the production of vascular endothelial growth factor and reduced the migration, adhesion, and invasiveness of osteosarcoma (OS) cells in vitro. The in vivo effects of ZOL were investigated by using a murine model of spontaneous lung metastasis. The higher dose of ZOL (80 microg/kg three times/week) inhibited the growth of OS at the primary site, accompanied by inhibition of neovascularization in the tumor. Interestingly, while the lower dose of ZOL (80 microg/kg once a week) could not inhibit the growth of OS at the primary site, it significantly prevented lung metastasis.
authors.affiliation: Department of Orthopedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Koto
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Horie
authors.forename: Shinya
authors.initials: S
authors.lastname: Kimura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Murata
authors.forename: Tomoya
authors.initials: T
authors.lastname: Sakabe
authors.forename: Takaaki
authors.initials: T
authors.lastname: Matsui
authors.forename: Motonobu
authors.initials: M
authors.lastname: Watanabe
authors.forename: Souichi
authors.initials: S
authors.lastname: Adachi
authors.forename: Taira
authors.initials: T
authors.lastname: Maekawa
authors.forename: Shinji
authors.initials: S
authors.lastname: Fushiki
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Kubo
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004164:Diphosphonates / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007093:Imidazoles / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000378:metabolism / Q000517:prevention & control / Q000556:secondary*; D051379:Mice; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D017368:Protein Prenylation; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis; D000077211:Zoledronic Acid
chemical_list: D004164:Diphosphonates; D007093:Imidazoles; D042461:Vascular Endothelial Growth Factor A; D000077211:Zoledronic Acid