title: Immunosensor for the determination of azidothymidine: its utilization as detector in a sequential injection analysis system.
abstract: An amperometric immunosensor based on graphite paste (graphite powder and paraffin oil) has been constructed for the assay of azidothymidine (AZT). The graphite paste is impregnated with anti-AZT. The immunosensor can be reliably used for the assay of AZT in its pharmaceutical formulation. The potential used for AZT assay was 435 mV vs Ag/AgCl electrode. The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new assay. Due to its reliability, the immunosensor was successfully used as a detector in a sequential injection analysis system, and gave reliable results for on-line assay of AZT purity in raw material and AZT contents in pharmaceutical formulations.
authors.affiliation: Department of Chemistry, University of Pretoria, Pretoria 0002, South Africa. raluca.stefan@chem.up.ac.za
authors.forename: Raluca-Ioana
authors.initials: RI
authors.lastname: Stefan
authors.forename: Rahel Girmai
authors.initials: RG
authors.lastname: Bokretsion
authors.forename: Jacobus Frederick
authors.initials: JF
authors.lastname: van Staden
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein