Flow-injection fluorimetric analysis of sulfamethoxazole in pharmaceutical preparations and biological fluids.
A fluorimetric procedure for the determination of sulfamethoxazole using flow injection analysis is proposed. A two channel system is optimized for the fluorescent reaction with o-phthaldialdehyde and beta-mercaptoethanol. The detection limit is 0.007 mug/ml. No fluorescence is generated by the metabolite N-acetylated sulfamethoxazole. The method is applied to the determination of sulfamethoxazole in pharmaceuticals, urine and bovine serum samples without matrix interference problems.
Erroz
C L
CL
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, E-30071 Murcia, Spain.
Viñas
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Córdoba
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MH