Application of response surface methodology (RSM) for optimization of nutrient supplementation for Cr (VI) removal by Aspergillus lentulus AML05.
Response surface methodology (RSM) involving central composite design (CCD) was employed to optimize the concentrations of different media components for growth and Cr (VI) removal (100 mg l(-1) initial concentration) by Aspergillus lentulus AML05 at pH 6.5. The interaction between five variables i.e. glucose, K(2)HPO(4), MgSO(4), yeast extract, NH(4)NO(3) was studied and modelled. The statistical analysis of the results showed that in the range studied, yeast extract had a significant effect on Cr (VI) removal and production of fungal biomass. The optimum combination predicted via RSM was confirmed through experiment, whereby almost complete removal (99.8%) of Cr (VI) was obtained within 120 h. The validation of these results in terms of field applicability was also tested by treating industrial effluent supplemented with key media components.
Sharma
Shweta
S
Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi , India.
Malik
Anushree
A
Satya
Santosh
S
D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D002857:Chromium / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry; D007220:Industrial Waste / Q000517:prevention & control; D008722:Methods
D003470:Culture Media; D007220:Industrial Waste; D002857:Chromium