Identification of the human paragonimiasis causative agent in Lao People's Democratic Republic.
To assess the species of human paragonimiasis in Lao People's Democratic Republic, 6 ovum samples from 6 native confirmed paragonimiasis patients were examined with polymerase chain reaction (PCR) amplifying the internal transcribed spacer 2 (ITS2). The PCR products were sequenced, and a homology search was performed using the GenBank. All 6 sequences were identical with Paragonimus heterotremus ITS2. Our work suggests that P. heterotremus may be the main etiological agent of human paragonimiasis in this locality.
Yahiro
S
S
Taketomi Clinic, Fukuoka City 8100032, Japan.
Habe
S
S
Duong
V
V
Odermatt
P
P
Barennes
H
H
Strobel
M
M
Nakamura
S
S
D000818:Animals; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D006801:Humans; D007812:Laos / Q000453:epidemiology; D010063:Ovum / Q000145:classification; D010237:Paragonimiasis / Q000453:epidemiology / Q000469:parasitology*; D010238:Paragonimus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D013183:Sputum / Q000469:parasitology
D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers