title: Light-emitting-diode-induced fluorescence detector for capillary electrophoresis using optical fiber with spherical end.
abstract: A simple fluorescence detector for capillary electrophoresis (CE) using a blue light-emitting-diode (LED) as excitation source is constructed and evaluated. An optical fiber was used to collect the fluorescence, and a flat end of the fiber was modified to spherical end, resulting in 50% increase of efficiency over the flat end. A simple device for optical alignment of the fibers and capillary column was designed. The concentration and mass detection limits for fluorescein were 1.8x10(-7) mol l(-1) and 4.3 femol, respectively.
authors.affiliation: Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, P.O. Box 100, Dalian 116012, People's Republic of China.
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Yang
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Guan