Enhanced systemic matrix metalloproteinase response in Helicobacter pylori gastritis.
BACKGROUND
Helicobacter pylori causes chronic gastritis, peptic ulcer disease, and is the most important risk factor for non-cardia gastric cancer, and has been shown to upregulate matrix metalloproteinases (MMPs) in infected gastric mucosa. MMPs are proteolytic enzymes regulated by tissue inhibitors of metalloproteinases (TIMPs).


OBJECTIVE
We set up this study to find out whether H. pylori gastritis induces systemic MMP response.


METHODS
Serum samples were collected from patients undergoing gastroscopy; 26 patients had H. pylori gastritis and 18 were H. pylori-negative controls with normal gastric mucosa. Serum MMP levels were analysed by enzyme-linked immunosorbent assay.


RESULTS
Significantly elevated serum levels of collagenase-2 (MMP-8), gelatinase B (MMP-9), neutrophil elastase (NE), and myeloperoxidase (MPO), and reduced serum levels of gelatinase A (MMP-2) and TIMP-1 were demonstrated in patients with H. pylori gastritis as compared to H. pylori-negative controls. No significant differences were shown in serum matrilysin-1 (MMP-7) levels.


CONCLUSIONS
For the first time, we show enhanced MMP-8 response in H. pylori infection together with other neutrophil degranulation products (MMP-9, MPO, NE). Elevated circulating neutrophil degranulation product levels in serum of H. pylori-positive patients reflect accelerated proteolysis and oxidative stress, and may contribute to extraintestinal sequelae, such as cardiovascular diseases.
Rautelin
Hilpi I
HI
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki and HUSLAB, Helsinki University Central Hospital Laboratory, Finland. hilpi.rautelin@helsinki.fi
Oksanen
Aino M
AM
Veijola
Lea I
LI
Sipponen
Pentti I
PI
Tervahartiala
Taina I
TI
Sorsa
Timo A
TA
Lauhio
Anneli
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005756:Gastritis / Q000201:enzymology* / Q000382:microbiology; D016481:Helicobacter Infections / Q000201:enzymology* / Q000382:microbiology; D016480:Helicobacter pylori; D006801:Humans; D019272:Leukocyte Elastase / Q000097:blood; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000097:blood; D020784:Matrix Metalloproteinase 8 / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D020782:Matrix Metalloproteinases / Q000097:blood*; D053505:Matrix Metalloproteinases, Secreted / Q000097:blood; D008875:Middle Aged; D009195:Peroxidase / Q000097:blood; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood; D015854:Up-Regulation
D019715:Tissue Inhibitor of Metalloproteinase-1; D009195:Peroxidase; D019272:Leukocyte Elastase; D020782:Matrix Metalloproteinases; D053505:Matrix Metalloproteinases, Secreted; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9