Impact of filter paper on fluorescence measurements of buffered saline filtrates.
Fluorescent contaminants have been observed when stock solutions of phosphate buffered saline solutions at each of three pH values (2.2, 7.5, and 12.5) are analyzed after passing through commercially available filter paper. The filtrate's fluorescence was observed to exhibit a maximum signal at 440.0 nm when excited at 365 nm. Detection of trace components could have significant implications in the design and implementation of sample processing protocols when using fluorescence.
Danielson
Timothy L
TL
Department of Chemistry and Biochemistry, New Mexico State University, Box 30001 MSC 3C, Las Cruces, New Mexico, NM 88003 8001, USA.
Rayson
Gary D
GD
Anderson
Dean M
DM
Estell
Rick
R
Fredrickson
Ed L
EL
Green
Barbara S
BS