title: Electrochemical detection of in situ adriamycin oxidative damage to DNA.
abstract: Adriamycin intercalation and in situ interaction with double helix DNA was investigated using a voltammetric DNA-biosensor. Oxidation and reduction of adriamycin molecules intercalated in double helix DNA were investigated in order to understand the in vivo mechanism of action with this anti-neoplasic drug. The results showed that the interaction of adriamycin with DNA is potential-dependent causing contact between DNA guanine and adenine bases and the electrode surface such that their oxidation is easily detected. A mechanism for adriamycin reduction and oxidation in situ when intercalated in double helix DNA immobilised onto the glassy carbon electrode surface is presented and the formation of the mutagenic 8-oxoguanine explained.
authors.affiliation: Departamento de Quimica, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, 3004-535 Coimbra, Portugal.
authors.forename: A M
authors.initials: AM
authors.lastname: Oliveira-Brett
authors.forename: M
authors.initials: M
authors.lastname: Vivan
authors.forename: I R
authors.initials: IR
authors.lastname: Fernandes
authors.forename: J A P
authors.initials: JA
authors.lastname: Piedade