[The study of preimplantation development of mice embryos labelled with green fluorescent protein gene (EGFp)].
One of the crucial problems of developmental biology is the study of mechanisms of regulation of gene expression in early embryogenesis. Here we studied dynamics of mosaic appearance of a marker fluorescent protein in in vitro developing mice embryo derived from zygotes after microinjections to male pronuclei of cloned DNA fragment carrying EGFP under control of different promoters. Main attention was paid to initial stages of development, when structural rearrangements and reprogramming of both parental genomes, activation of zygotic genes, and control of development by embryo genome take place.
Sorokin
A V
AV
Noniashvili
E M
EM
Kidgotko
O V
OV
Sasina
L K
LK
Aleĭnikova
T D
TD
Shavlovskiĭ
M M
MM
Gorodetskiĭ
S I
SI
Dyban
A P
AP
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D008845:Microinjections; D009030:Mosaicism / Q000196:embryology*; D019076:Transgenes; D015053:Zygote / Q000166:cytology / Q000378:metabolism
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins