Suspension bead array branch migration displacement assay for rapid STR analysis.
STR analysis is commonly used in forensic and genetic studies. STRs are currently discriminated based on size, primarily by gel- and column-based approaches. Hybridization-based approaches have the potential to allow high-throughput analysis of STRs; however, development of such approaches has been limited by the difficulty in discriminating between STRs of similar length. We have recently described several innovations to enable STR analysis using an array-based hybridization approach for high- throughput STR analysis. Here we extend that approach by incorporating the array into microspheres and adding a discriminatory branch migration displacement step. This microsphere-based platform uses Luminex xMAP technology and improves the sensitivity, selectivity, and speed of the assay. We demonstrate the feasibility, speed, and reliability of the assay for STR detection by correctly analyzing two STR loci in 20 forensic DNA samples of known STR type. The multiplex, bead-based approach provides a high-throughput and more portable STR analysis.
Villablanca
Andrea
A
Stanford Genome Technology Center, Stanford University, Palo Alto, CA, USA.
Karhanek
Miloslav
M
Caramuta
Stefano
S
Yu
Heng
H
Jejelowo
Olufisayo
O
Webb
Chris D
CD
Pourmand
Nader
N
D006801:Humans; D018895:Microsatellite Repeats; D008863:Microspheres; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013535:Suspensions
D015345:Oligonucleotide Probes; D013535:Suspensions