title: Synthesis of a new water-soluble oligo(phenylenevinylene) containing a tyrosine moiety for tyrosinase activity detection.
abstract: A new water-soluble oligo(phenylenevinylene) containing a tyrosine unit (OPV-Tyr) was synthesized as a fluorescent probe to optically detect tyrosinase activity. Upon the addition of tyrosinase, the tyrosine moiety was oxidized to quinone, which quenched the fluorescence of OPV-Tyr via intramolecular electron transfer from the phenylenevinylene unit to the quinone site. OPV-Tyr was elaborated to detect tyrosinase activity both in aqueous buffer solution and in agarose gel.
authors.affiliation: Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Xuli
authors.initials: X
authors.lastname: Feng
authors.forename: Fude
authors.initials: F
authors.lastname: Feng
authors.forename: Minghui
authors.initials: M
authors.lastname: Yu
authors.forename: Fang
authors.initials: F
authors.lastname: He
authors.forename: Qingling
authors.initials: Q
authors.lastname: Xu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Tang
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Li
authors.forename: Daoben
authors.initials: D
authors.lastname: Zhu
mesh_terms: D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D011145:Polyvinyls / Q000138:chemical synthesis* / Q000737:chemistry*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014443:Tyrosine / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011145:Polyvinyls; C530822:oligo(p-phenylene vinylene); D014867:Water; D011092:Polyethylene Glycols; D014443:Tyrosine; D014442:Monophenol Monooxygenase