Preparation and evaluation of a large-volume radial flow monolithic column.
In this study, a 38 mL monolith with homogeneous porous structure was produced by a single polymerization from glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) in the presence of porogens and an initiator. The uniform temperature distribution within the reaction system was achieved by adding reactant mixture continuously and enhancing the heat transfer ability of the polymerization system. Homogeneous porous structure in the monolith was proved by SEM and the pore size distribution profiles measured by mercury intrusion porosimetry. Experimental results from proteins separation indicated that the dynamic capacity and resolution of radial flow monolithic column were independent of flow rates. Furthermore, the pressure drop on the column was linearly dependent on the flow rate and did not exceed 1.7 MPa even at a flow rate of 50 mL/min, which proved that the prepared monolith could be used in the quick separation and preparation of biopolymers.
Yang
Changlong
C
School of Chemical Engineering, Dalian University of Technology, Dalian, China.
Wei
Yuanlong
Y
Zhang
Qinghe
Q
Zhang
Weibing
W
Li
Tong
T
Hu
Haoquan
H
Zhang
Yukui
Y