title: Ratio of peripheral nervous tissues in tongues, skeletal muscles and intestines in cows.
abstract: It is suggested that pathogenic prion protein (PrP(sc) may accumulate in peripheral nervous tissues in addition to the specified-risk materials. To assess the risk of PrP(sc) accumulation in peripheral tissues, the present study was designed to study the ratio of peripheral nervous tissues in tongues, skeletal muscles and intestines in 2-week- and 59-month-old cows. The nervous tissues could be detected by immunohistochemistry using antibody Neurofilament 150. After immunostaining with Neurofilament 150, the photographs taken from each section were applied for Scion Image software. Then, the ratio of nervous tissues in tongues, skeletal muscles and intestines were calculated. The highest ratio of peripheral nervous tissues was observed in intestines, the second highest ratio was found in tongues, and the lowest ratio was recognized in skeletal muscles in both 2-week- and 59-month-old cows. The ratio of nervous tissues in intestines was approximately 2.5 fold and 10 fold, compared to that in skeletal muscles in 2-week- and 59-month-old cows, respectively. These results suggest that intestines and tongues may have a higher risk of PrP(sc) accumulation, compared to skeletal muscles.
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: XiaoBo
authors.initials: X
authors.lastname: Zhu
authors.forename: Shogo
authors.initials: S
authors.lastname: Matoba
authors.forename: Kenshiro
authors.initials: K
authors.lastname: Hara
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Ano
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Naoki
authors.initials: N
authors.lastname: Tsunekawa
authors.forename: Yoshiakira
authors.initials: Y
authors.lastname: Kanai
authors.forename: Noboru
authors.initials: N
authors.lastname: Manabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Onodera
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kurohmaru
mesh_terms: D000375:Aging; D000818:Animals; D002417:Cattle / Q000033:anatomy & histology*; D005260:Female; D007150:Immunohistochemistry; D007422:Intestines / Q000294:innervation*; D018482:Muscle, Skeletal / Q000294:innervation*; D017933:Peripheral Nervous System / Q000033:anatomy & histology*; D014059:Tongue / Q000294:innervation*