title: A multicommuted flow system for sequential spectrophotometric determination of hydrosoluble vitamins in pharmaceutical preparations.
abstract: A multicommuted flow system is proposed for spectrophotometric determination of hydrosoluble vitamins (ascorbic acid, thiamine, riboflavine and pyridoxine) in pharmaceutical preparations. The flow manifold was designed with computer-controlled three-way solenoid valves for independent handling of sample and reagent solutions and a multi-channel spectrophotometer was employed for signal measurements. Periodic re-calibration as well as the standard addition method was implemented by using a single reference solution. Linear responses (r=0.999) were obtained for 0.500-10.0 mgl(-1) ascorbic acid, 2.00-50.0 mgl(-1) thiamine, 5.00-50.0 mgl(-1) riboflavine and 0.500-8.00 mgl(-1) pyridoxine. Detection limits were estimated as 0.08 mgl(-1) (0.5 mumoll(-1)) ascorbic acid, 0.8 mgl(-1) (2 mumoll(-1)) thiamine, 0.2 mgl(-1) (0.5 mumoll(-1)) riboflavine and 0.1 mgl(-1) (0.9 mumoll(-1)) pyridoxine at 99.7% confidence level. A mean sampling rate of 60 determinations per hour was achieved and coefficients of variation of 1% (n=20) were estimated for all species. The mean reagent consumption was 25-fold lower in relation to flow-based procedures with continuous reagent addition. Average recoveries between 95.6 and 100% were obtained for commercial pharmaceutical preparations. Results agreed with those obtained by reference methods at 95% confidence level. The flow system is suitable for application in quality control processes and in dissolution studies of vitamin tablets.
authors.affiliation: Departamento de Quimica, Universidade Federal de São Carlos, P.O. Box 676, 13560-970 São Carlos, SP, Brazil.
authors.forename: Fábio R P
authors.initials: FR
authors.lastname: Rocha
authors.forename: Orlando
authors.initials: O
authors.lastname: Fatibello Filho
authors.forename: Boaventura F
authors.initials: BF
authors.lastname: Reis