Direct fluorescent detection of chromatographically separated amino-acids by means of 9-isothiocyanatoacridines.
A new method is presented for fluorescent detection of amino-acids separated on silica gel R plates, by spraying with a 0.1% solution of 9-isothiocyanatoacridine derivatives in dichloromethane or benzene, heating and examining in ultraviolet light at 254 and 336 nm. This method is useful for routine analysis owing to its selectivity and sensitivity.
Sârbu
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Faculty of Mechanical Technology, Polytechnic Institute, 3400 Cluj-Napoca, Romania.
Mărutoiu
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Vlassa
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Liteanu
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