The neuroprotective effect of dental pulp cells in models of Alzheimer's and Parkinson's disease.
Aim of the present study was to investigate the neuroprotective effect of dental pulp cells (DPCs) in in vitro models of Alzheimer and Parkinson disease. Primary cultures of hippocampal and ventral mesencephalic neurons were treated for 24 h with amyloid beta (Abeta(1-42)) peptide 1-42 and 6-OHDA, respectively. DPCs isolated from adult rat incisors were previously cultured in tissue culture inserts and added to the neuron cultures 2 days prior to neurotoxin treatment. Cell viability was assessed by the MTT assay. The co-culture with DPCs significantly attenuated 6-OHDA and Abeta(1-42)-induced toxicity in primary cultures of mesencephalic and hippocampal neurons, and lead to an increase in neuronal viability in untreated cultures, suggesting a neurotrophic effect in both models. Furthermore, human dental pulp cells expressed a neuronal phenotype and produced the neurotrophic factors NGF, GDNF, BDNF, and BMP2 shown by microarray screening and antibody staining for the representative proteins. DPCs protected primary neurons in in vitro models of Alzheimer's and Parkinson's disease and can be viewed as possible candidates for studies on cell-based therapy.
Apel
C
C
Department and Institute of Psychiatry, University of São Paulo, São Paulo, Brazil. capel@ukaachen.de
Forlenza
O V
OV
de Paula
V J R
VJ
Talib
L L
LL
Denecke
B
B
Eduardo
C P
CP
Gattaz
W F
WF
D018663:Adrenergic Agents / Q000633:toxicity*; D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D003782:Dental Pulp / Q000166:cytology* / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000166:cytology; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008636:Mesencephalon / Q000166:cytology; D046228:Microarray Analysis / Q000379:methods; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D016627:Oxidopamine / Q000633:toxicity*; D010446:Peptide Fragments / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats
D018663:Adrenergic Agents; D016229:Amyloid beta-Peptides; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D016627:Oxidopamine