Gardenia jasminoides protects against cerulein-induced acute pancreatitis.
OBJECTIVE
To investigate the effect of Gardenia jasminoides (GJ) on cerulein-induced acute pancreatitis (AP) in mice.


METHODS
C57BL/6 mice weighing 18-20 g were divided into three groups. (1) Normal saline-treated group, (2) treatment with GJ at a dose of 0.1 g/kg, (3) treatment with GJ at a dose of 1 g/kg. GJ was administered orally (n = 6 per group) for 1 wk. Three hours later, the mice were given an intraperitoneal injection of cerulein (50 microg/kg), a stable cholecystokinin (CCK) analogue, every hour for a total of 6 h as described previously. The mice were sacrificed at 6 h after completion of cerulein injections. Blood samples were obtained to determine serum amylase, lipase and cytokine levels. The pancreas was rapidly removed for morphologic examination and scoring. A portion of pancreas was stored at -70 degree and prepared for the measurement of tissue myeloperoxidase (MPO) activity, an indicator of neutrophil sequestration, and for reverse-transcriptase PCR (RT-PCR) and real-time PCR measurements.


RESULTS
Treatment with GJ decreased significantly the severity of pancreatitis and pancreatitis-associated lung injury. Treatment with GJ attenuated the severity of AP compared with saline-treated mice, as shown by reduction in pancreatic edema, neutrophil infiltration, serum amylase and lipase levels, serum cytokine levels, and mRNA expression of multiple inflammatory mediators.


CONCLUSIONS
These results suggest that GJ attenuated the severity of AP as well as pancreatitis-associated lung injury.
Jung
Won-Seok
WS
Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, Jeonju, 570-749 Jeonbuk, South Korea.
Chae
Young-Seok
YS
Kim
Do-Yun
DY
Seo
Sang-Wan
SW
Park
Hee-Je
HJ
Bae
Gi-Sang
GS
Kim
Tae-Hyeon
TH
Oh
Hyo-Jeong
HJ
Yun
Ki-Jung
KJ
Park
Rae-Kil
RK
Kim
Jong-Suk
JS
Kim
Eun-Cheol
EC
Hwang
Sung-Yeon
SY
Park
Sung-Joo
SJ
Song
Ho-Joon
HJ
D000208:Acute Disease; D000284:Administration, Oral; D000681:Amylases / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001835:Body Weight; D002108:Ceruletide; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D032062:Gardenia; D018836:Inflammation Mediators / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008049:Lipase / Q000097:blood; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D055370:Lung Injury / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000187:drug effects; D009929:Organ Size; D010179:Pancreas / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D010936:Plant Extracts; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D002108:Ceruletide; D009195:Peroxidase; D008049:Lipase; D000681:Amylases