title: Nucleotide sequence and genome organization of a member of a new and distinct Caulimovirus species from dahlia.
abstract: A distinct caulimovirus, associated with dahlia mosaic, was cloned and sequenced. The caulimovirus, tentatively designated as dahlia common mosaic virus (DCMV), had a double-stranded DNA genome of ca. 8 kb. The genome organization of DCMV was found to be typical of members of the genus Caulimovirus and consisted of six major open reading frames (ORFs), ORFs I-VI, and one minor ORF, ORF VII. Sequence comparisons with the DNA genomes of two known caulimoviruses isolated from dahlia, Dahlia mosaic virus (DMV) and an endogenous caulimovirus, DMV-D10, showed that DCMV is a member of a distinct caulimovirus species, with sequence identities among various ORFs ranging from 25 to 80%.
authors.affiliation: Department of Plant Pathology, Washington State University, PO Box 646430, Pullman, WA 99164-6430, USA. hrp@wsu.edu
authors.forename: H R
authors.initials: HR
authors.lastname: Pappu
authors.forename: K L
authors.initials: KL
authors.lastname: Druffel
authors.forename: R
authors.initials: R
authors.lastname: Miglino
authors.forename: A R
authors.initials: AR
authors.lastname: van Schadewijk
mesh_terms: D001483:Base Sequence; D017796:Caulimovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D031944:Dahlia / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*