Microchip capillary electrophoresis with amperometric detection for several carbohydrates.
Microchip capillary electrophoresis (muCE) with amperometric detection at Cu electrode benefited fast separation and direct detection of carbohydrates. The working electrode of 50-mum Cu wire attached nearly against the channel outlet-4mum, where it benefited collecting detection current and suppressing overwhelming noise. The use of alkaline medium was essential to separating and detecting carbohydrates, which dissociated into the sensitive alcolate anions. The 10-cm serpentine chip, though lengthening the migration time, it provided better efficiency. Sucrose, cellobiose, glucose, and fructose migrated from the outlet in 400s +2000V. The linear calibration plots ranging from 10 to 1000muM with regression coefficients better than 0.996 were obtained. The injection-to-injection reproducibility of 1.24% (n=7) for glucose in peak current and 0.6% for migration times were excellent. The detection limit was low, down to 2.3muM for glucose (S/N=3) or 27.6attomole in mass detection.
Lee
Hui-Ling
HL
Department of Chemistry, Fu-Jen Catholic University, No. 510 Road Chang Cheng, Shinchung, Hsinchuang, 242 Taipei, Taiwan, ROC.
Chen
Show-Chuen
SC