Fluorescence energy transfer between Acridine Orange and Safranine T and its application in the determination of DNA.
The fluorescence energy transfer (FET) between Acridine Orange and Safranine T, two intercalators of DNA, was studied in this paper. The FET efficiency between Acridine Orange and Safranine T is higher and the critical distance, R(0), is longer in the intercalated state than in the free one. A new method for the determination of calf thymus DNA (ctDNA) was presented. The linear range of the calibration curve is (0 approximately 1.1)x10(-5) mol l(-1) in bases for ctDNA, and the limit of detection is 2.6x10(-7) mol l(-1).
Cao
Y
Y
Department of Chemistry, Nankai University, Tianjin 300071, People's Republic of China.
He
X
X
Gao
Z
Z
Peng
L
L