title: Pharmacokinetics of cimifugin in rat plasma after oral administration of the extract of Saposhnikovia divaricatae root. Determination of cimifugin by high performance liquid chromatography coupled with solid phase extraction.
abstract: High performance liquid chromatography (HPLC) coupled with the solid phase extraction method was developed for determining cimifugin (a coumarin derivative; one of Saposhnikovia divaricatae's constituents) in rat plasma after oral administration of Saposhnikovia divaricatae extract (SDE), and the pharmacokinetics of cimifugin either in SDE or as a single compound was investigated. The HPLC analysis was performed on a commercially available column (4.6 mm x 200 mm, 5 pm) with the isocratic elution of solvent A (Methanol) and solvent B (Water) (A:B=60:40) and the detection wavelength was set at 250 nm. The calibration curve was linear over the range of 0.100-10.040 microg/mL. The limit of detection was 30 ng/mL. At the rat plasma concentrations of 0.402, 4.016, 10.040 microg/mL, the intra-day precision was 6.21%, 3.98%, and 2.23%; the inter-day precision was 7.59%, 4.26%, and 2.09%, respectively. The absolute recovery was 76.58%, 76.61%, and 77.67%, respectively. When the dosage of SDE was equal to the pure compound calculated by the amount of cimifugin, it was found to have two maximum peaks while the pure compound only showed one peak in the plasma concentration-time curve. The pharmacokinetic characteristics of SDE showed the superiority of the extract and the properties of traditional Chinese medicine.
authors.affiliation: College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, The People's Republic of China. wxj@hljucm.net
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
authors.forename: Zenghui
authors.initials: Z
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Haitao
authors.initials: H
authors.lastname: Lv
authors.forename: Hongying
authors.initials: H
authors.lastname: Guan
authors.forename: Xiangcai
authors.initials: X
authors.lastname: Meng
authors.forename: Haifeng
authors.initials: H
authors.lastname: Sun
authors.forename: Guangmei
authors.initials: G
authors.lastname: Zhang
authors.forename: Hongxin
authors.initials: H
authors.lastname: Cao
mesh_terms: D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002867:Chromones / Q000097:blood* / Q000493:pharmacokinetics*; D006207:Half-Life; D008297:Male; D018517:Plant Roots / Q000737:chemistry; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
chemical_list: D002867:Chromones; C533198:cimifugin