title: Free Bioverit II implants coated with a nanoporous silica layer in a mouse ear model-- a histological study.
abstract: The objective of this study is to evaluate the suitability of a mouse middle ear model for testing ossicular replacement materials. Twenty-four BALB/c mice are implanted with the bioglass-ceramic Bioverit II which is coated with a silica-nanostructure or with plain Bioverit II as a control. After 2, 6, and 12 weeks, 4 mice per group are sacrificed and both complete petrous bones are analyzed histologically. All implants revealed in situ an incipient growth of thin connective tissue layers over the surface, followed by a spreading of epithelial cells. The osseogenic response which is increasing with time is more intense in the coated Bioverit II specimens. The absence of inflammatory cells suggests an excellent biocompatibility of the silica nano structure. As the results are comparable to a study with the same materials in rabbits, the mouse model described is highly suitable for evaluation of new ossicular replacement materials. Additionally, by gene expression analysis a more detailed insight into cellular interactions of the middle ear is offered.
authors.affiliation: Department of Otolaryngology, Medical School Hannover, Carl-Neubergstr. 1, 30625 Hannover, Germany.
authors.forename: Julia C
authors.initials: JC
authors.lastname: Vogt
authors.forename: Gudrun
authors.initials: G
authors.lastname: Brandes
authors.forename: Nina
authors.initials: N
authors.lastname: Ehlert
authors.forename: Peter
authors.initials: P
authors.lastname: Behrens
authors.forename: Ingo
authors.initials: I
authors.lastname: Nolte
authors.forename: Peter P
authors.initials: PP
authors.lastname: Mueller
authors.forename: Thomas
authors.initials: T
authors.lastname: Lenarz
authors.forename: Martin
authors.initials: M
authors.lastname: Stieve
mesh_terms: D000818:Animals; D002516:Ceramics / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004432:Ear, Middle / Q000637:transplantation* / Q000648:ultrastructure; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D049329:Nanostructures / Q000737:chemistry; D009998:Ossicular Prosthesis; D010012:Osteogenesis; D016062:Porosity; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry*; D013997:Time Factors
chemical_list: C022107:Bioglass; D020099:Coated Materials, Biocompatible; D012822:Silicon Dioxide