Glucose oxidation and PQQ-dependent dehydrogenases in Gluconobacter oxydans.
Gluconobacter oxydans is famous for its rapid and incomplete oxidation of a wide range of sugars and sugar alcohols. The organism is known for its efficient oxidation of D-glucose to D-gluconate, which can be further oxidized to two different keto-D-gluconates, 2-keto-D-gluconate and 5-keto-D-gluconate, as well as 2,5-di-keto-D-gluconate. For this oxidation chain and for further oxidation reactions, G. oxydans possesses a high number of membrane-bound dehydrogenases. In this review, we focus on the dehydrogenases involved in D-glucose oxidation and the products formed during this process. As some of the involved dehydrogenases contain pyrroloquinoline quinone (PQQ) as a cofactor, also PQQ synthesis is reviewed. Finally, we will give an overview of further PQQ-dependent dehydrogenases and discuss their functions in G. oxydans ATCC 621H (DSM 2343).
Hölscher
Tina
T
Fachgebiet Technische Biochemie, Institut fur Biotechnologie der Technischen Universität Berlin, Berlin, Germany.
Schleyer
Ute
U
Merfort
Marcel
M
Bringer-Meyer
Stephanie
S
Görisch
Helmut
H
Sahm
Hermann
H
D003067:Coenzymes / Q000378:metabolism; D020600:Gluconobacter oxydans / Q000201:enzymology* / Q000235:genetics; D005947:Glucose / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D045542:PQQ Cofactor / Q000235:genetics / Q000378:metabolism*
D003067:Coenzymes; D045542:PQQ Cofactor; D010088:Oxidoreductases; D005947:Glucose