[Antimutagenic activity of serotoninergic system and its mechanisms in Acipenser gueldenstaedti persicus and Carassius auratus].
The paper deals with antimutagenic body activity and its underlying mechanisms. The experiments carried out on the one-year old sturgeons (Acipenser gueldenstaedti persicus) and goldfish (Carassius auratus) have shown that intramuscular administration of serotoninmodulated anticonsolidation protein (SMAP) leads to a twofold decrease of erythrocyte mutagenic alterations (the micronuclear test, p < 0.01) caused by action of benthic deposits (0.8 ml/l, 3 days) polluted with industrial wastes. Exposure of goldfish in water contaminated with oil (500 mg/l, 3 days) led to a sharp rise of the content of the 70 kDa brain protein fraction (p < 0.001); these water-soluble proteins are assumed to belong to heat shock proteins (HSP). At the same time, in the brain of the studied animals there was observed a simultaneous increase of the SMAP content (p < 0.001). After 3 h, intracerebral SMAP administration to goldfish increased significantly the 90 kDa protein fraction content (p < 0.01), probably HSP90, in the electrophoretic profiles of the brain water-soluble proteins. Thus, the obtained results indicate that the body serotoninergic system has the antimutagenic activity providing protection of cells from action of harmful environmental factors by an enhancement of synthesis of proteins suggested to belong to HSP.
Mekhtiev
A A
AA
Movsum-zade
S K
SK
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D005399:Fishes / Q000235:genetics / Q000378:metabolism*; D019015:Geologic Sediments; D006054:Goldfish / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D009152:Mutagenicity Tests; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D010578:Petroleum / Q000633:toxicity; D012701:Serotonin / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity
D018840:HSP70 Heat-Shock Proteins; D009419:Nerve Tissue Proteins; D010578:Petroleum; D014874:Water Pollutants, Chemical; D012701:Serotonin