title: Subtypes of melanocytes and melanoma cells distinguished by their intercellular contacts: heterotypic adherens junctions, adhesive associations, and dispersed desmoglein 2 glycoproteins.
abstract: In the tissue integration of melanocytes and melanoma cells, an important role is attributed to cell adhesion molecules, notably the cadherins. In cultured melanoma cells, we have previously described a more heterogeneous repertoire of cadherins than normal, including some melanoma subtypes synthesizing the desmosomal cadherin, desmoglein 2, out of the desmosomal context. Using biochemical and immunological characterization of junctional molecules, confocal laser scanning, and electron and immunoelectron microscopy, we now demonstrate homo- and heterotypic cell-cell adhesions of normal epidermal melanocytes. In human epidermis, both in situ and in cell culture, melanocytes and keratinocytes are connected by closely aligned membranes that are interspersed by small puncta adhaerentia containing heterotypic complexes of E- and P-cadherin. Moreover, melanocytes growing in culture often begin to synthesize desmoglein 2, which is dispersed over extended areas of intimate adhesive cell-cell associations. As desmoglein 2 is not found in melanocytes in situ, we hypothesize that its synthesis is correlated with cell proliferation. Indeed, in tissue microarrays, desmoglein 2 has been demonstrated in a sizable subset of nevi and primary melanomas. The biological meanings of these cell-cell adhesion molecule arrangements, the possible diagnostic and prognostic significance of these findings, and the implications of the heterogeneity types of melanomas are discussed.
authors.affiliation: Helmholtz Group for Cell Biology, German Cancer Research Center, Heidelberg, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Rickelt
authors.forename: Werner W
authors.initials: WW
authors.lastname: Franke
authors.forename: Yvette
authors.initials: Y
authors.lastname: Doerflinger
authors.forename: Sergij
authors.initials: S
authors.lastname: Goerdt
authors.forename: Johanna M
authors.initials: JM
authors.lastname: Brandner
authors.forename: Wiebke K
authors.initials: WK
authors.lastname: Peitsch
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism* / Q000648:ultrastructure; D000328:Adult; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion; D002450:Cell Communication; D002478:Cells, Cultured; D018920:Coculture Techniques; D051301:Desmoglein 2 / Q000378:metabolism*; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D047468:Immunoprecipitation; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008544:Melanocytes / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D016253:Microscopy, Immunoelectron; D009506:Nevus / Q000378:metabolism / Q000473:pathology; D016612:Paraffin Embedding; D021381:Protein Transport; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D046888:Tissue Array Analysis
chemical_list: D015820:Cadherins; D051301:Desmoglein 2