Inoculation of white-tailed deer (Odocoileus virginianus) with Ap-V1 Or NY-18 strains of Anaplasma phagocytophilum and microscopic demonstration of Ap-V1 In Ixodes scapularis adults that acquired infection from deer as nymphs.
Four white-tailed deer were inoculated with either the Ap-V1 or NY-18 strain of Anaplasma phagocytophilum. Ixodes scapularis nymphs were then allowed to acquistion feed on the inoculated deer and molt to adults. Only an Ap-V1 infected deer was infected persistently and able to infect nymphal Ixodes scapularis. Molted adult ticks maintained Ap-V1 infection as demonstrated by PCR and microscopy. We report, for the first time, a morphologic description of A. phagocytophilum in I. scapularis.
Reichard
Mason V
MV
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA. mason.reichard@okstate.edu
Roman
Raul Manzano
RM
Kocan
Katherine M
KM
Blouin
Edmour F
EF
de la Fuente
José
J
Snider
Timothy A
TA
Heinz
Rebecca E
RE
West
Misti D
MD
Little
Susan E
SE
Massung
Robert F
RF
D041081:Anaplasma phagocytophilum / Q000145:classification* / Q000502:physiology*; D000712:Anaplasmosis / Q000382:microbiology* / Q000635:transmission; D000818:Animals; D003670:Deer / Q000382:microbiology*; D018884:Ixodes / Q000382:microbiology*; D009758:Nymph / Q000382:microbiology