Molecular detection of Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' Infection in cats by direct PCR using whole blood without DNA extraction.
Detection of hemotropic Mycoplasma spp. infection was attempted in cats by PCR using whole blood without DNA extraction. A total 46 of 54 (85%) cats with suspected Mycoplasma spp. infection showed a positive reaction, corresponding completely with the results of standard PCR testing. The direct PCR assay was sensitive enough to detect more than 0.0061% parasitemia for ;C. M. haemominutum' and 0.0075% parasitemia for M. haemofelis. These data indicate that the direct PCR assay might be sufficient for use as a tool in clinical examinations.
Watanabe
Masashi
M
Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, Sagamihara City, Kanagawa, Japan.
Hisasue
Masaharu
M
Souma
Takehisa
T
Ohshiro
Shuichi
S
Yamada
Takatsugu
T
Tsuchiya
Ryo
R
D000740:Anemia / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D002371:Cat Diseases / Q000097:blood / Q000175:diagnosis / Q000382:microbiology*; D002415:Cats; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006400:Hematocrit / Q000662:veterinary; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary
D004269:DNA, Bacterial