Determination of urinary oxidative DNA damage marker 8-hydroxy-2'-deoxyguanosine and the association with cigarette smoking.
8-hydroxy-2'-deoxyguanosine (8OHdG) has been widely used as a biomarker of oxidative DNA damage in both animal models and human studies. To evaluate the effect of cigarette smoking on oxidative stress, we studied the levels of urinary 8OHdG from smokers and non-smokers and investigated the association with cigarette smoking. The urinary 8OHdG concentrations were determinated by capillary electrophoresis with end-column amprometric detection (CE-AD) after a single-step solid phase extraction (SPE), and then quantitatively expressed as a function of creatinine excretion. To increase the concentration sensitivity, a dynamic pH junction was used and the focusing effect was obvious when using 30mM phosphate (pH 6.50) as sample matrix. The limit of detection is 4.3nM (signal-to-noise ratio S/N=3). The relative standard deviation (R.S.D.) was 1.1% for peak current, and 2.3% for migration time. Based on the selected CE-AD method, it was found that the mean value of urinary 8OHdG levels in the smokers significantly higher than that in non-smokers (31.4+/-18.9 nM versus 14.4+/-7.6 nM , P=0.0004; 23.5+/-21.3 mug g (-)(1) creatinine versus 12.6+/-13.2 mug g (-)(1) creatinine, P=0.028).
Yao
Qing-Hong
QH
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, ZhongShan Road 161, Dalian 116011, PR China.
Mei
Su-Rong
SR
Weng
Qian-Feng
QF
Zhang
Pu-Duen
PD
Yang
Qing
Q
Wu
Cai-Ying
CY
Xu
Guo-Wang
GW