title: Determination by hplc of trifluoroacetate levels in plasma and urine of patients anaesthetized with halothane.
abstract: A new method is described for determination of trifluoroacetic acid (TFA) in biological fluids. Optimum extraction is achieved by addition of 18-crown-6 ether and acidification of the sample. The 4-bromomethyl-7-methoxycoumarin derivatives of the carboxylic acid are prepared and a sample is subjected to HPLC. A linear analytical curve of peak area against TFA concentrations ranging between 0.2 and 20 mug ml is obtained, and the minimum detectable concentration is estimated to be 0.1 mug ml .
authors.affiliation: Laboratoires de Toxicologie, Hydrologie et Hygiène, Faculté de Pharmacie, Rue du Professeur Laguesse, 59045 Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Imbenotte
authors.forename: A
authors.initials: A
authors.lastname: Brice
authors.forename: F
authors.initials: F
authors.lastname: Erb
authors.forename: J M
authors.initials: JM
authors.lastname: Haguenoer