title: Injection of An. stephensi embryos to generate malaria-resistant mosquitoes.
abstract: The introduction of exogenous genes into the genomes of mosquitoes requires microinjection techniques tailored to the specific species of interest. This video protocol demonstrates a method used by the James laboratory to microinject DNA constructs into Anopheles stephensi embryos for the generation of transformed mosquitoes. Techniques for preparing microinjection needles, collecting and preparing embryos and performing the microinjection are illustrated.
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California, Irvine, USA. olle.terenius@uci.edu
authors.forename: Olle
authors.initials: O
authors.lastname: Terenius
authors.forename: Jennifer
authors.initials: J
authors.lastname: Juhn
authors.forename: Anthony A
authors.initials: AA
authors.lastname: James
mesh_terms: D000818:Animals; D000852:Anopheles / Q000196:embryology* / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage; D018014:Gene Transfer Techniques; D007113:Immunity, Innate / Q000235:genetics*; D008288:Malaria / Q000517:prevention & control*; D008845:Microinjections / Q000379:methods
chemical_list: D004247:DNA