Fluorometric determination of nitrite in natural waters with 3-aminonaphthalene-1,5-disulphonic acid by flow-injection analysis.
Nitrite in river and sea-water was determined fluorometrically by flow-injection analysis. In acidic medium, nitrite was reacted with 3-aminonaphthalene-1,5-disulphonic acid (C-acid) to form the diazonium salt, which was converted into the fluorescent azoic acid salt in an alkaline medium. The carrier stream, into which the sample solution was injected, was distilled water. The reagent solution stream, which contained C-acid, EDTA and hydrochloric acid, was mixed with the carrier stream in a 13-m length of Teflon tubing (bore 0.5 mm) maintained at 90 degrees in a thermostatic bath. After passing through the mixing coil, the stream was mixed with an alkaline solution. The fluorescence intensity (excited at 365 nm) was measured at 470 nm. The detection limit (S N = 3) was 1 x 10(-9)M (14 ng 1 . nitrite-nitrogen) and the RSD of 10 injections of 10(-6)M nitrite was 0.4%. Analyses can be done at a rate of up to 45 hr .
Motomizu
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Department of Chemistry, Faculty of Science, Okayama University, Tsushima-naka, Okayama-shi 700, Japan.
Mikasa
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Tôei
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