The influence of X-rays on the structural studies of peroxide-derived myoglobin intermediates.
In recent years, the awareness of potential radiation damage of metal centers in protein crystals during crystallographic data collection has received increasing attention. The radiation damage can lead to radiation-induced changes and reduction of the metal sites. One of the research fields where these concerns have been comprehensively addressed is the study of the reaction intermediates of the heme peroxidase and oxygenase reaction cycles. For both the resting states and the high-valent intermediates, the X-rays used in the structure determination have given undesired side effects through radiation-induced changes to the trapped intermediates. However, X-rays have been used to generate and trap the peroxy/hydroperoxy state in crystals. In this review, the structural work and the influence of X-rays on these intermediates in myoglobin are summarized and viewed in light of analogous studies on similar intermediates in peroxidases and oxygenases.
Hersleth
Hans-Petter
HP
University of Oslo, Department of Molecular Biosciences, P. O. Box 1041 Blindern, N-0316 Oslo.|University of Oslo, Department of Chemistry, P. O. Box 1033 Blindern, N-0315 Oslo.
Hsiao
Ya-Wen
YW
Lund University, Department of Theoretical Chemistry, P. O. Box 124, S-221 00 Lund.
Ryde
Ulf
U
Lund University, Department of Theoretical Chemistry, P. O. Box 124, S-221 00 Lund.
Görbitz
Carl Henrik
CH
University of Oslo, Department of Chemistry, P. O. Box 1033 Blindern, N-0315 Oslo.
Andersson
K Kristoffer
KK
University of Oslo, Department of Molecular Biosciences, P. O. Box 1041 Blindern, N-0316 Oslo.
D018360:Crystallography, X-Ray; D009211:Myoglobin / Q000737:chemistry / Q000528:radiation effects; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry / Q000528:radiation effects; D011487:Protein Conformation; D014965:X-Rays
D009211:Myoglobin; C048685:peroxymyoglobin; D009195:Peroxidase