Utilization of monolinolein as a substrate for conjugated linoleic acid production by Bifidobacterium breve LMC 520 of human neonatal origin.
This study was designed to isolate bifidobacteria from human intestines that efficiently converts monolinolein, a monoglyceride form of linoleic acid, into conjugated linoleic acid (CLA), as well as to optimize culture conditions for improving CLA production during milk fermentation. Among 150 screened neonatal bifidobacteria, Bifidobacterium breve LMC 520 showed the highest CLA-producing ability and was tested with different types of fat substrates at various concentrations to determine the optimal conditions for CLA production. Monolinolein was tested as a substrate for CLA production. The incubation time optimized for CLA production was 24 h, and CLA production was proportionally increased with monolinolein concentration. The incubation of LMC 520 with commercial starter strains caused minimal reduction in CLA production. Our results demonstrate that the CLA-producing ability of B. breve LMC 520 could offer beneficial effects when utilized as a starter culture for the development of functional dairy products.
Choi
Nag Jin
NJ
Hanwoo Experiment Station, National Institute of Animal Science, Pyongchang, Korea.
Park
Hui Gyu
HG
Kim
Young Jun
YJ
Kim
In Hwan
IH
Kang
Hye Soon
HS
Yoon
Chil Suk
CS
Yoon
Ho Geun
HG
Park
Su-Il
SI
Lee
Jae Woo
JW
Chung
Soo Hyun
SH
D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism*; D001942:Breast Feeding; D018920:Coculture Techniques; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D005989:Glycerides / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007779:Lactobacillus acidophilus; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*; D008892:Milk / Q000378:metabolism; D048249:Streptococcus thermophilus; D013997:Time Factors
D005989:Glycerides; D044243:Linoleic Acids, Conjugated; C048627:monolinolein