Applications of enzyme-catalysed reactions in trace analysis-V Determination of zinc and calcium by their activation of the apo-enzyme of calf-intestinal alkaline phosphatase.
The activation of the apo-enzyme of calf-intestinal alkaline phosphatase by zinc in the presence and absence of magnesium 4 x 10(-4)M) is applied to the determination of 6-65 ng and 65-650 ng of zinc, respectively. The activation of the apo-enzyme by calcium in the presence of 1.4 x 10(-7)M zinc is used to determine 1-4 mug of calcium. The mechanism of the re-activation effects is discussed.
Townshend
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Chemistry Department, The University, P.O. Box 363, Birmingham 15, England.
Vaughan
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