Characterization of uveitis induced by use of a single intravitreal injection of bacterial lipopolysaccharide in cats.
OBJECTIVE
To investigate the use of a single intravitreal injection of bacterial lipopolysaccharide (LPS) to experimentally induce uveitis in cats.


METHODS
7 young male European shorthair cats that were considered physically and ophthalmologically healthy.


METHODS
In each cat, LPS was injected intravitreally into 1 eye; the contralateral eye was injected with the preparation vehicle. During a period of 45 days, both eyes were evaluated by means of clinical evaluation; assessment of the integrity of the blood-aqueous humor barrier (determined via measurement of protein concentration and cell content in samples of aqueous humor); functional analysis (via electroretinography); and following euthanasia, histologic examination of the retinas.


RESULTS
In LPS-treated eyes, several clinical signs were observed until day 45 after injection. Compared with vehicle-treated eyes, intraocular pressure was significantly lower and protein concentration and the number of infiltrating cells were significantly higher in LPS-treated eyes. Mean amplitudes of scotopic electroretinographic a- and b-waves were significantly reduced in eyes injected with LPS, compared with findings in eyes injected with vehicle. At 45 days after injection, LPS-induced alterations in photoreceptors and the middle portion of the retina were detected histologically.


CONCLUSIONS
Results indicated that a single intravitreal injection of LPS in eyes of cats induced clinical, biochemical, functional, and histologic changes that were consistent with the main features of naturally occurring uveitis. This technique may be a useful tool in the investigation of new treatment strategies for uveitis in cats.
Del Sole
María J
MJ
Laboratorio de Fisiología del Sistema Nervioso y Endocrinología, Departamento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, 7000, Argentina.
Sande
Pablo H
PH
Felipe
Antonio E
AE
Fernandez
Diego C
DC
Keller Sarmiento
María I
MI
Aba
Marcelo A
MA
Rosenstein
Ruth E
RE
D000704:Analysis of Variance; D000818:Animals; D002371:Cat Diseases / Q000139:chemically induced* / Q000473:pathology*; D002415:Cats; D004596:Electroretinography / Q000662:veterinary; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D014605:Uveitis / Q000139:chemically induced / Q000473:pathology / Q000662:veterinary*
D008070:Lipopolysaccharides