title: Detection and genotyping of an infectious pancreatic necrosis virus from asymptomatic rainbow trout (Oncorhynchus mykiss) facilities in Mexico.
abstract: BACKGROUND
  Infectious pancreatic necrosis virus (IPNV) is a birnavirus that causes a lethal disease in both hatchery-reared juvenile salmonids and other non-salmonid fishes. IPNV has been classified into seven genogroups based on the analysis of the VP2/NS junction region of the viral A RNA segment.
  
  
  METHODS
  Ten organisms from two trout-rearing farms were used for viral isolation in RTG-2 cells. Cells were inoculated with samples from spleen, kidneys and pyloric caeca. The viral isolate was initially identified by electron microscopy, and confirmed by a commercial ELISA, RT-PCR and sequencing. A phylogenetic tree was constructed based on the deduced amino acids sequence of VP2.
  
  
  RESULTS
  An IPNV genogroup 1, labeled as EdoMex07, was isolated from a pool of renal tissues of five asymptomatic trout. The amino acid sequence analysis of VP2 showed that this IPNV presented the putative VP2 residue (221) already described in asymptomatic trout carriers.
  
  
  CONCLUSIONS
  The EdoMex07 IPNV isolate belongs to genogroup 1, and has a VP2 phenotype which has been suggested to be involved in the establishment of the carrier state. This EdoMex07 IPNV is currently used as the standard positive control for detection of IPNV in rainbow trout farms in Mexico.
authors.affiliation: Laboratory of Virology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, Mexico.
authors.forename: Luis
authors.initials: L
authors.lastname: Guerrero
authors.forename: Emma
authors.initials: E
authors.lastname: Herrera
authors.forename: Juana
authors.initials: J
authors.lastname: Salinas
authors.forename: Jesús
authors.initials: J
authors.lastname: Torres
authors.forename: Ana Bertha
authors.initials: AB
authors.lastname: Montero
authors.forename: Blanca
authors.initials: B
authors.lastname: Barrón
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000662:veterinary* / Q000821:virology; D002353:Carrier State / Q000662:veterinary* / Q000821:virology; D002432:Cecum / Q000821:virology; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D018061:Infectious pancreatic necrosis virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007668:Kidney / Q000821:virology; D008800:Mexico; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins