Overall alteration of circadian clock gene expression in the chestnut cold response.
Cold acclimation in woody plants may have special features compared to similar processes in herbaceous plants. Recent studies have shown that circadian clock behavior in the chestnut tree (Castanea sativa) is disrupted by cold temperatures and that the primary oscillator feedback loop is not functional at 4 degrees C or in winter. In these conditions, CsTOC1 and CsLHY genes are constantly expressed. Here, we show that this alteration also affects CsPRR5, CsPRR7 and CsPRR9. These genes are homologous to the corresponding Arabidopsis PSEUDO-RESPONSE REGULATOR genes, which are also components of the circadian oscillator feedback network. The practically constant presence of mRNAs of the 5 chestnut genes at low temperature reveals an unknown aspect of clock regulation and suggests a mechanism regulating the transcription of oscillator genes as a whole.
Ibañez
Cristian
C
Centro de Biotecnología y Genómica de Plantas, Departamento de Biotecnología, Universidad Politécnica de Madrid, E. T. S. Ingenieros de Montes, Madrid, Spain.
Ramos
Alberto
A
Acebo
Paloma
P
Contreras
Angela
A
Casado
Rosa
R
Allona
Isabel
I
Aragoncillo
Cipriano
C
D000064:Acclimatization / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D056926:CLOCK Proteins; D002940:Circadian Rhythm / Q000235:genetics; D003080:Cold Temperature; D029962:Fagaceae / Q000235:genetics* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000502:physiology; D010802:Phylogeny; D012097:Repressor Proteins; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
D029681:Arabidopsis Proteins; C523307:PRR5 protein, Arabidopsis; C523308:PRR7 protein, Arabidopsis; C514751:PRR9 protein, Arabidopsis; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D056926:CLOCK Proteins