Pluronic F-127 as a cell carrier for bone tissue engineering.
The objective of this study is to report the effect of Pluronic F-127 on osteoblast viability and phenotype maintenance in vitro. MG-63 cells are suspended in Pluronic F-127, and MTT assay, alkaline phosphatase activity, prostaglandin E(2) production, collagen-I, and cyclo-oxygenase-2 expression are assessed up to 6 days. Pluronic F-127 leads to a significant decrease in osteoblast viability throughout the 6-day experiment, without altering osteoblast phenotype. The addition of platelet-rich plasma to the polymer/cell construct leads to increased cell survival. When supplemented with bioactive factors, Pluronic F-127 could potentially be used as a cell carrier in bone tissue engineering.
Brunet-Maheu
Jean-Marc
JM
Orthopaedic Research Laboratory, Department of Orthopaedics, Centre hospitalier Sacré-Coeur, Montréal, Québec, Canada.
Fernandes
Julio C
JC
de Lacerda
Clemente A V
CA
Shi
Qin
Q
Benderdour
Mohamed
M
Lavigne
Patrick
P
D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D020442:Poloxamer / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
D018786:Bone Substitutes; D020442:Poloxamer