Disposable superoxide anion biosensor based on superoxide dismutase entrapped in silica sol-gel matrix at gold nanoparticles modified ITO electrode.
A novel disposable biosensor based on direct electron transfer of superoxide dismutase (SOD) was fabricated for the determination of superoxide anion. The biosensor was constructed by electrodeposition of gold nanoparticles (GNPs) on the indium tin oxide (ITO) electrode and then immobilization of SOD in silica sol-gel (SG) network in the presence of cysteine on GNPs/ITO modified electrode surface. The distribution of GNPs on ITO electrode surface was examined by scanning electron microscopy (SEM). The immobilized SOD exhibited high catalytical activity towards superoxide anion. Parameters affecting the performance of the biosensor were also investigated. A linear calibration curve was obtained over the range from 0.08 to 0.64 microM with a correlation coefficient of 0.9937. The resulted biosensors were demonstrated to possess striking analytical properties for superoxide anion determination, such as high sensitivity, good accuracy, and long-term stability. It provides a promising platform for the fabrication of disposable biosensors.
Wang
Yuan
Y
Department of Chemistry, Suzhou University, 215123, Suzhou, Jiangsu, People's Republic of China.
Wu
Ying
Y
Wang
Jianwen
J
Di
Junwei
J
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000706:statistics & numerical data; D055664:Electrochemical Techniques; D004566:Electrodes; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D006046:Gold; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D058428:Silica Gel; D012822:Silicon Dioxide; D013482:Superoxide Dismutase; D013481:Superoxides / Q000032:analysis*; D017971:Tin Compounds
D004800:Enzymes, Immobilized; D017971:Tin Compounds; D013481:Superoxides; D058428:Silica Gel; C109984:indium tin oxide; D006046:Gold; D012822:Silicon Dioxide; D013482:Superoxide Dismutase