[The comparison about inhibition of five Chinese herbal medicines to two different resources alpha-glycosidase].
OBJECTIVE
To compare alpha-glycosidase inhibitor microamount screening models which are established by two different resources alpha-glycosidase to screen the Chinese herbal medicines which have great alpha-glycosidase inhibition.


METHODS
Comparing the activities of two different resources alpha-glycosidase with glucose oxidase method, then establishing optimal reaction conditions. Extracting the water soluble compositions of Chinese gallnut, paeoniae radix, Glycyrrhiza, rhubarb, fructus by boiling ,then using the alpha-glycosidase inhibition model to check and compare their alpha-glycosidase inhibitions.


RESULTS
Five Chinese herbal medicines all had alpha-glycosidase inhibition, and the greatest was Chinese gallnut.


CONCLUSIONS
The activities of two resources alpha-glycosidase are certainly different, but they do not have significant influence on findings of screening alpha-glycosidase inhibitors in vitro. Water soluble compositions of five Chinese herbal medicines have alpha-glycosidase inhibition.
Zhang
Hai-Feng
HF
Department of Pharmacy, The First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710061, China.
Dong
Ya-Lin
YL
Hu
Sa-Sa
SS
Ren
Shao-Jun
SJ
D020909:Acarbose / Q000494:pharmacology; D000818:Animals; D029768:Cornus / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D029882:Glycyrrhiza uralensis / Q000737:chemistry; D007413:Intestinal Mucosa / Q000201:enzymology; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D029626:Ranunculaceae / Q000737:chemistry; D051381:Rats; D012250:Rheum / Q000737:chemistry; D013997:Time Factors; D000520:alpha-Glucosidases / Q000302:isolation & purification / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D065089:Glycoside Hydrolase Inhibitors; D000520:alpha-Glucosidases; D020909:Acarbose