Simultaneous determination of free-form amphetamine in rat's blood and brain by in-vivo microdialysis and liquid chromatography with fluorescence detection.
The determination of amphetamine in rat's blood and brain simultaneously by micordiaysis technique and HPLC/fluorescence derivatization is described. Microdiaysis is used to sample rat's blood and brain fluid and the amphetamine content is evaluated by HPLC/fluorescence derivatization assay. Dansyl chloride is used as fluorescence derivatization reagent. The detection limit, linearity and precision associated with this assay were evaluated. Pharmarcokinetic parameters of amphetamine in rat's blood and brain are also reported.
Fuh
Ming-Ren
MR
Department of Chemistry, Soochow University, PO Box 86-72, Taipei, Taiwan, ROC.
Lin
Hui-Tung
HT
Pan
Wynn H T
WH
Lin
Fun-Ru
FR