[Determination of "amino acids conflicts" and amino acids substitutions in primary structures of 41 human proteins by use the proteomic technologies].
Proteomic studies of some human tissues and organs (skeletal muscles, myometrium, motor zone of the brain, prostate), and also cultivated myoblasts revealed 41 of 300 identified proteins, in which the present of certain variants of amino acids ("conflicts") was recognized at several positions. Among the 93 registered amino acids "conflicts", seven cases represented the results of the protein polymorphisms caused by corresponding substitution of individual amino acid. Moreover, among prostate proteins the proteomic analysis revealed two isoforms of prostate-specific antigen, formed due to alternative splicing. Thus, our results have shown, that proteomic technologies allow to specify effectively the features of primary structures and to characterize various kinds of polymorphism in many human proteins.
Kovaleva
M A
MA
Kovalev
L I
LI
Eremina
L S
LS
Makarov
A A
AA
Burakova
M V
MV
Toropygin
I Iu
IIu
Serebriakova
M V
MV
Shishkin
S S
SS
Archakov
A I
AI
D000595:Amino Acid Sequence / Q000235:genetics; D019943:Amino Acid Substitution; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D009124:Muscle Proteins / Q000032:analysis / Q000235:genetics*; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics*; D009928:Organ Specificity / Q000502:physiology; D011110:Polymorphism, Genetic; D017430:Prostate-Specific Antigen / Q000032:analysis / Q000235:genetics*; D040901:Proteomics
D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D017430:Prostate-Specific Antigen