title: Genetic variability of Candida albicans isolates in a university hospital in Hungary.
abstract: The occurrence and genetic variability of Candida albicans isolates in a Hungarian hospital were examined. Among the 103 Candida isolates, 44 (42.7%) proved to be C. albicans species. Comparing with a previous study carried out in 2002, the percentage of infections caused by C. albicans decreased in Hungary in this period with an increasing incidence of non-albicans species, in accordance with the world-wide trend. The genetic variability of the isolates was examined using mitochondrial DNA (mtDNA), restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) analysis and electrophoretic karyotyping. The examined C. albicans isolates could be clustered into four groups based on their mtDNA profiles. The electrophoretic karyotypes of the isolates were mostly identical to that of the reference strain 1006, with the exception of mtDNA type II isolates. RAPD analysis could be used to cluster the isolates into different groups, but this clustering was not in complete agreement with their assignment to mtDNA types. Population genetic analyses of the data indicated low amounts of recombination among these C. albicans strains. None of the isolates exhibited decreased susceptibilities to 5-fluorocytosine.
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary CBS Fungal Biodiversity Centre, Utrecht, The Netherlands.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Farkas
authors.forename: Sándor
authors.initials: S
authors.lastname: Kocsubé
authors.forename: Mónika
authors.initials: M
authors.lastname: Tóth
authors.forename: Csaba
authors.initials: C
authors.lastname: Vágvölgyi
authors.forename: Judit
authors.initials: J
authors.lastname: Kucsera
authors.forename: János
authors.initials: J
authors.lastname: Varga
authors.forename: Ilona
authors.initials: I
authors.lastname: Pfeiffer
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000935:Antifungal Agents / Q000494:pharmacology; D002176:Candida albicans / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D005260:Female; D014644:Genetic Variation; D006785:Hospitals, University; D006801:Humans; D006814:Hungary; D008297:Male; D008875:Middle Aged; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D055815:Young Adult
chemical_list: D000935:Antifungal Agents