Peroxidase-like activity on organic hydroperoxides of ion-exchange resins modified with metal-porphine analogues and analytical application for determination of linoleate hydroperoxide.
The ion-exchange resins modified with metal-porphyrins and -phthalocyanines (M-P(r)) have been found to exhibit a peroxidase (POD)-like activity on organic peroxides in a reaction wherein a quinoid dye is formed from phenol and 4-aminoantipyrine. Among them, Mn- and Co-P(r) exhibited stronger activity than hemoglobin and Fe-P(r), and hence were expected to be practically useful as a solid catalyst for the determination of linoleate hydroperoxide (LOOH). In addition, a resin modified with Co(3+)-phthalocyanine tetrasulfonate (Co-PCS(r)) lacks POD-like activity on hydrogen peroxide in contrast with Mn-P(r). We, therefore, concluded that Co-PCS(r) is superior to both Mn-P(r) and hemoglobin as a solid catalyst on organic hydroperoxides, and developed a new method for the determination of LOOH.
Mifune
M
M
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan.
Tai
T A
TA
Iwado
A
A
Akizawa
H
H
Oda
J
J
Motohashi
N
N
Saito
Y
Y