title: Selective extraction of bacterial DNA from the surfaces of macroalgae.
abstract: A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing.
authors.affiliation: School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, The University of New South Wales, Sydney 2052, Australia.
authors.forename: Catherine
authors.initials: C
authors.lastname: Burke
authors.forename: Staffan
authors.initials: S
authors.lastname: Kjelleberg
authors.forename: Torsten
authors.initials: T
authors.lastname: Thomas
mesh_terms: D004269:DNA, Bacterial / Q000302:isolation & purification*; D008967:Molecular Biology / Q000379:methods*; D000461:Rhodophyta / Q000382:microbiology*; D012680:Sensitivity and Specificity; D044445:Ulva / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial