Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts.
One of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin-derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 (P<0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15 microM) induce cell cycle arrest at the G0/G1 phase.
Choresca
Casiano H
CH
Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea.
Koo
Ok Jae
OJ
Oh
Hyun Ju
HJ
Hong
So Gun
SG
Gomez
Dennis K
DK
Kim
Ji Hyung
JH
Lee
Byeong Chun
BC
Park
Se Chang
SC
D000704:Analysis of Variance; D000818:Animals; D018929:Cell Culture Techniques; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D016193:G1 Phase; D006054:Goldfish; D011687:Purines / Q000378:metabolism / Q000494:pharmacology; D016192:Resting Phase, Cell Cycle; D000077546:Roscovitine; D013217:Starvation
D011687:Purines; D000077546:Roscovitine