title: In vitro biocompatibility and degradation of terpolyester 3HB-co-4HB-co-3HHx, consisting of 3-hydroxybutyrate, 4-hydroxybutyrate and 3-hydroxyhexanoate.
abstract: A terpolyester consisting of 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB) and 3-hydroxyhexanoate (3HHx), abbreviated as P(3HB-co-4HB-co-3HHx), was studied for possible application as an implant biomaterial. L929 mouse fibroblasts, MC3T3-E1 murine osteoblasts and a human cell line of immortalized human keratinocyte (HaCat cells) were used to study the biocompatibility of P(3HB-co-4HB-co-3HHx). Cell morphology and cell activity were studied using scanning electron microscopy (SEM) and the MTT assay, respectively. All three cell types showed higher activities when grown on films of P(3HB-co-4HB-co-3HHx) compared with their growth on poly(lactic acid) (PLA), co-polyester PHBHHx films and on polylysine-coated plates (blank), respectively. The three cell types grown on the terpolyester also demonstrated a well-spread cell shape and large number of pseudopods due to strong cell-cell and cell-material interactions. It was clearly observed that P(3HB-co-4HB-co-3HHx) had a much faster degradation rate than PHBHHx after 15 weeks of incubation in phosphate-buffered saline under dynamic conditions. The results proved that the terpolyester had favorable biocompatibility and biodegradability compared with the well-studied polyesters PLA and PHBHHx.
authors.affiliation: Multidisciplinary Research Center, Shantou University, Shantou, Guangdong, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D000818:Animals; D002208:Caproates / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006885:Hydroxybutyrates / Q000737:chemistry*; D019344:Lactic Acid / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology; D013499:Surface Properties; D013997:Time Factors
chemical_list: D002208:Caproates; D006885:Hydroxybutyrates; D011091:Polyesters; D011108:Polymers; C107955:poly(4-hydroxybutanoate); C045051:3-hydroxyhexanoic acid; C111420:4-hydroxybutyric acid; D019344:Lactic Acid; C033616:poly(lactide); D020155:3-Hydroxybutyric Acid