Analytical study of the phosphorescence of pyrimidine derivatives in frozen aqueous solution.
Phosphorescence excitation and emission spectra, phosphorescence lifetimes, phosphorimetric analytical curves and limits of detection were determined at 77K in 10/90 v/v methanol/water solution for seven pyrimidine derivatives. The effect of pH on the phosphorescence intensity indicated an improvement of the sensitivity of the method in basic medium (pH reverse similar11). Low limits of detection, between 10(-5) and 10(-8)M, were obtained. The effect of sodium iodide on the phosphorescence efficiency led to heavy-atom enhancement factors ranging from 1.1 to 9.6, depending on the molecular structure and the pH conditions.
Aaron
J J
JJ
Department of Chemistry, University of Florida, Gainesville, Florida 32611, U.S.A.
Fisher
R
R
Winefordner
J D
JD