[Osteoprotegerin mRNA/receptor activator of NF-kappaB ligand mRNA expressions in bone tissues of glucocorticoid-induced osteonecrosis of the femoral head].
OBJECTIVE
To investigate the expression levels of osteoprotegerin (OPG) and receptor activator of NF-kappaB ligand (RANKL) mRNAs in bone tissues of the femoral head of the patients suffering glucocorticoid-induced osteonecrosis of the femoral head (ONFH), and to discuss the relationship between OPG/RANKL and ONFH.


METHODS
Between March 2007 and March 2008, bone tissues of the femoral head were collected as the experimental material from 35 patients suffering ONFH (experimental group) and from 21 patients suffering fracture of femoral neck (control group). The ratio of men to women in both groups was 4 : 3, whose age was 41-70 years old (55.34 on average in the experimental group and 55.33 on average in the control group). The experimental group received over 3 weeks' glucocorticoid treatment or more than 1 week's high-dose glucocorticoid treatment in recent 2 years, while the control group never received more than 1 week's hormone treatment. In the two groups, the microstructure of bone tissues of the femoral head was detected by HE staining and the bone tissue total RNA was extracted, and then the expression levels of OPG mRNA and RANKL mRNA were examined by real-time quantitative PCR (RTQ-PCR) for each sample.


RESULTS
HE staining: bone trabeculae and bone units were replaced by interrupted bone fragments, which were surrounded by many inflammatory granulation tissues and few osteocytes were seen in bone lacunae in the experimental group. In the control group, bone trabeculae and bone units were made by complete lamellar bones which surrounded blood vessels and osteocytes were seen in lacunae. RTQ-PCR testing: in the experimental group, OPG mRNA and RANKL mRNA were 1.35 +/- 0.42 and 4.36 +/- 1.35, respectively, while in the control group they were 1.78 +/- 0.63 and 3.49 +/- 1.02, respectively. The expression level of OPG mRNA in the experimental group was significantly lower than that in the control group, and the expression level of RANKL mRNA of the former was significantly higher than the latter. The OPG mRNA/ RANKL mRNA ratio in the experiment group (0.34 +/- 0.16) was significantly lower than that in the control group (0.54 +/- 0.20), and there was significant difference (P < 0.05).


CONCLUSIONS
The glucocorticoid-induced ONFH may be related to the expression levels of OPG mRNA/RANKL mRNA in bone tissues.
Wang
Jianzhong
J
Department of Orthopaedics, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an Shaanxi 710004, PR China. wjzwan@yahoo.com.cn
Wang
Kunzheng
K
Shi
Zhibin
Z
Zhang
Mingyu
M
D000328:Adult; D000368:Aged; D005260:Female; D005271:Femur Head Necrosis / Q000139:chemically induced / Q000378:metabolism*; D005938:Glucocorticoids / Q000009:adverse effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism*; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000235:genetics / Q000378:metabolism*
D005938:Glucocorticoids; D016328:NF-kappa B; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B