Antioxidant and hepatoprotective activity of a lichen Usnea ghattensis in vitro.
Antioxidative and hepatoprotective activity of a cultured lichen Usnea ghattensis has been studied. The methanolic extract of cultured lichen U. ghattensis showed good antioxidant activity by preventing lipid peroxidation by 67% and 86% in Trolox-equivalent antioxidant capacity at 20 microg/ml. It also showed superoxide, 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, and hydroxyl radical-scavenging activity, 89%, 89.6%, 94.8%, and 89.6%, respectively, and found levels higher then that known for the synthetic antioxidants butylated hydroxytoluene, butylated hydroxyanisol, and quercetin at 20 microg/ml concentration. The cultured lichen extract also showed hepatoprotection against ethanol-induced toxicity in the mice liver slice culture model by a significant decrease in the antioxidant enzymes, glutathione peroxidase, catalase, and superoxide dismutase, along with a decrease in lipid peroxidation and lactate dehydrogenase release.
Verma
Neeraj
N
Plant Science Division, Agharkar Research Institute, Pune, MS, India.
Behera
B C
BC
Makhija
Urmila
U
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D000431:Ethanol / Q000633:toxicity; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology; D020949:Usnea / Q000737:chemistry*
D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D020011:Protective Agents; D000431:Ethanol; D007770:L-Lactate Dehydrogenase