[Influence of Wnt-1 recombinant adenovirus on differentiation of human epidermal stem cells].
OBJECTIVE
To observe the influence of Wnt-1 recombinant adenovirus on differentiation tendency of human epidermal stem cells.


METHODS
Wnt-1 recombinant adenovirus was transduced into hESCs (E group), while normal hESCs were used as control (C) group. The diameter, proliferation,and labeling molecular expression of hESC were determined. The content of MMP-2 and MMP-7 in supernate were also assayed.


RESULTS
There was no obvious difference in diameter of hESC between two groups. The density of hESC in E group was (1.45 +/- 0.09) x 10(5)/mL, which was obviously higher than that in C group [(1.18 +/- 0.10) x 10(5)/mL, P < 0.05]. There were no obvious differences in expression of markers between two groups,including keratin 5 (KS), K6, K7, KS, K14, CD44, carcinoembryonic-like antigen (CEAA), ER, PR (P > 0.05) ,while the expression of K 10 was different among groups [(60 +/- 3)% in E group, 0 in C group], also K18 [(34.3 +/- 2.1)% in E group vs. (13.8 +/- 1.7)% in C group, P < 0.05], and K19 [(17.1 +/- 1.8)% in E group vs. (24.4 +/- 1.5)% in C group, P < 0.05].The contents of MMP-2 and MMP-7 in E group were higher than those in C group (P < 0.01).


CONCLUSIONS
Wnt-1 recombinant adenovirus can induce the differentiation of hESCs to glandular epithelium-like cells.
Li
Yuan-chao
YC
Department of Dermatology, Daping Hospital, Field Surgery Institute, the Third Military Medical University, Chongqing 400042, PR China.
Wu
Jin-jin
JJ
Bi
Jian-jun
JJ
Yang
Tao
T
Yang
Gui-hong
GH
Wang
Bing-qiang
BQ
D000256:Adenoviridae / Q000235:genetics*; D002454:Cell Differentiation; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology* / Q000821:virology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*; D051155:Wnt1 Protein / Q000235:genetics*
D051155:Wnt1 Protein; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2