Real-time PCR in a plastic chip based on solid state FRET.
We show a novel solid state optical detection platform, integrated in a plastic biochip, based on colloidal nanocrystal FRET donors. The approach exploits a "smart" polymeric layer with both optical and biorecognition properties that allows real-time monitoring of biomolecular interactions and quantitative analyses of real-time PCR. The proposed strategy, demonstrated here for DNA detection, may open interesting perspectives for a wide range of applications, such as for proteomic studies.
Sabella
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National Nanotechnology Laboratory of CNR-INFM, IIT Research Unit, Via per Arnesano 73100 Lecce, Italy.
Vecchio
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Cingolani
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Rinaldi
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Pompa
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PP
D003102:Colloids; D004247:DNA / Q000032:analysis; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation* / Q000379:methods; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D012680:Sensitivity and Specificity
D003102:Colloids; D004247:DNA