Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide.
Enhanced adhesion and migration of osteoblastic cells on titanium (Ti) surface is believed to increase the success rate of implant therapy. A GRGDSP peptide derived from fibronectin was coated on Ti surfaces using a tresyl chloride activation technique, and then MC3T3-E1 osteoblastic cells were cultured on the Ti surfaces. After 15 days, total RNA was isolated from the cells and gene expression level were analyzed by Affymetrix GeneChip system. The expression levels of many genes in MC3T3-E1 cells cultured on GRGDSP-coated Ti surface were altered when compared to uncoated Ti. In particular, the elevated mRNA levels of bone sialoprotein (BSP) and osteocalcin (OC) were successfully confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. In light of the results obtained, GRGDSP-coated Ti presented the potential of evolving into a useful biomaterial for successful implant therapy.
Yamamichi
Nobuyuki
N
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry, Matsudo, 2-870-1, Sakaecho-Nishi, Matsudo, Chiba, Japan.
Pugdee
Kamolparn
K
Chang
Wei-Jen
WJ
Lee
Sheng-Yang
SY
Yoshinari
Masao
M
Hayakawa
Tohru
T
Abiko
Yoshimitsu
Y
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D005353:Fibronectins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D058574:Integrin-Binding Sialoprotein; D051379:Mice; D008855:Microscopy, Electron, Scanning; D020411:Oligonucleotide Array Sequence Analysis; D009842:Oligopeptides / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D015675:Osteocalcin / Q000032:analysis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000032:analysis; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; C546872:Ibsp protein, mouse; D058574:Integrin-Binding Sialoprotein; D009842:Oligopeptides; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D015675:Osteocalcin; C058161:glycyl-arginyl-glycyl-aspartyl-seryl-proline; D014025:Titanium