Proteomic analysis of androgen-independent growth in low and high passage human LNCaP prostatic adenocarcinoma cells.
The present study compared the proteomic characteristics of a low passage number (L-33) and high passage number (H-81) LNCaP cell clone. Marked differences in protein expression were noted in the response of L-33 and H-81 cells to androgens. To investigate if regulation of these proteins was androgen-dependent, expression of the androgen receptor was silenced via small interfering RNA. Consistent with the proteomic data, abrogation of androgen receptor production in H-81 cells resulted in the reversed expression level into L-33 cells compared with non-treated H-81 LNCaP cells. The results clarify the progression into an androgen-independent phenotype.
Youm
Yun Hee
YH
Department of Urology, Eulji Medical Center, Eulji University School of Medicine, Seoul, Korea.
Kim
Seyoon
S
Bahk
Young Yil
YY
Yoo
Tag Keun
TK
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D000728:Androgens / Q000494:pharmacology*; D002457:Cell Extracts; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002999:Clone Cells; D017292:Doxazosin / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D020543:Proteome; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism
C410798:AR protein, human; D000728:Androgens; D002457:Cell Extracts; D009363:Neoplasm Proteins; D020543:Proteome; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011944:Receptors, Androgen; D017292:Doxazosin