A silver electrode in the potentiometric titration of thiols.
A silver wire immersed in a thiol solution gives a potential responsive to the thiol concentration, and is a sensitive indicator electrode in the potentiometric titration of thiols with mercury(II) chloride, p-chloromercuryphenyl sulphonate, and silver nitrate at pH 4.5-9.5, 7-9.5 and 9.5 respectively. Titrations of simple thiols such as cysteine or a protein such as albumin are equally successful, but the potential break was smaller for the protein. The end-point could be determined within an increment of titrant equal to 5 nmole of thiol. An inert atmosphere is needed for titration at pH 7.
Tombara
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Department of Radiation Biology and Biophysics University of Rochester School of Medicine and Dentistry Rochester, New York 14620, U.S.A.
Koval
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