Free radical fragmentation of cardiolipin by cytochrome c.
The effect of cytochrome c (cyt c) on degradation of cardiolipin in its polar part was investigated in cardiolipin/phosphatidylcholine (CL/PC) liposomes incubated with cyt c/H(2)O(2)/and (or) ascorbate by high-performance thin layer chromatography and MALDI-TOF mass spectrometry. It has been shown that phosphatidic acid (PA) and phosphatidylhydroxyacetone (PHA) were formed in the system under conditions where hydrogen peroxide favours a release of heme iron from cyt c. The formation of PA and PHA occurs via an OH-induced fragmentation taking place in the polar moiety of cardiolipin. Formation of fragmentation products correlated with the loss of CL in CL/PC liposomes incubated with cyt c/H(2)O(2)/ascorbate or with Cu(2+)/H(2)O(2)/ascorbate.
Yurkova
Irina
I
Research Institute for Physical Chemical Problems, Belarusian State University, Minsk, Belarus.
Huster
Dominik
D
Arnhold
Juergen
J
D000818:Animals; D002308:Cardiolipins / Q000378:metabolism*; D002417:Cattle; D045304:Cytochromes c / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002308:Cardiolipins; D005609:Free Radicals; D008081:Liposomes; D010713:Phosphatidylcholines; D045304:Cytochromes c; D006861:Hydrogen Peroxide