Synthesis of a new water-soluble oligo(phenylenevinylene) containing a tyrosine moiety for tyrosinase activity detection.
A new water-soluble oligo(phenylenevinylene) containing a tyrosine unit (OPV-Tyr) was synthesized as a fluorescent probe to optically detect tyrosinase activity. Upon the addition of tyrosinase, the tyrosine moiety was oxidized to quinone, which quenched the fluorescence of OPV-Tyr via intramolecular electron transfer from the phenylenevinylene unit to the quinone site. OPV-Tyr was elaborated to detect tyrosinase activity both in aqueous buffer solution and in agarose gel.
Feng
Xuli
X
Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
Feng
Fude
F
Yu
Minghui
M
He
Fang
F
Xu
Qingling
Q
Tang
Hongwei
H
Wang
Shu
S
Li
Yuliang
Y
Zhu
Daoben
D
D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D011145:Polyvinyls / Q000138:chemical synthesis* / Q000737:chemistry*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014443:Tyrosine / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D005456:Fluorescent Dyes; D011145:Polyvinyls; C530822:oligo(p-phenylene vinylene); D014867:Water; D011092:Polyethylene Glycols; D014443:Tyrosine; D014442:Monophenol Monooxygenase