Assessing the binding selectivity of molecularly imprinted polymer artificial antibodies by mass spectrometry-based profiling system.
Molecularly imprinted polymer (MIP) is a technique for generating polymer-bearing biomimetic receptors. It offers several advantages to the research such as analysis, sensors, extraction, or preconcentration of components. Myoglobin is known to be an important biological index for the diagnosis of cardiac diseases. The purpose of this research was to optimize the formation of myoglobin-imprinted polymer (Myo-MIP) and develop a mass spectrometry-based profiling system for assessing the binding selectivity of artificial antibodies formed by Myo-MIP. Experimental results showed that myoglobin and albumin were bound/absorbed onto Myo-MIP chips and not to nonimprinted polymer (NIP) chips. Other proteins, such as histidine-rich glycoprotein, immunoglobulins, proapolipoprotein, and leech-derived tryptase inhibitor, were also observed but with less reproducibility from the chips.
Liao
Pao-Chi
PC
Department of Environmental and Occupational Health, National Cheng Kung University, Tainan, Taiwan.
Tyan
Yu-Chang
YC
Wang
Chung-Yao
CY
Hsu
Jing-Fang
JF
Chou
Tse-Chuan
TC
Lin
Hsin-Yi
HY
D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D054802:Molecular Imprinting; D009211:Myoglobin / Q000032:analysis* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding
D009211:Myoglobin; D011108:Polymers