Ammonia-dependent differential regulation of the gene cluster that encodes ammonia monooxygenase in Nitrosococcus oceani ATCC 19707.
Molecular analysis of the ammonia monooxygenase-encoding gene cluster in Nitrosococcus oceani revealed that the amo genes are differentially expressed from three promoters dependent on the external ammonia concentration. Whereas a distal amoRCABD operon promoter, pC1, is active only in the presence of ammonia, identified proximal amoC (pC2) and amoA (pA) sigma-70-type promoters are constitutive. Promoter region pC2 also contains a consensus sequence for binding of RpoN, usually involved in nitrogen starvation response. Additional regulation of transcript stability by anti-termination and mRNA degradation are proposed and discussed.
El Sheikh
Amal F
AF
Department of Biology, Evolutionary and Genomic Microbiology Laboratory, University fo Louisville, Louisville, KY, USA.
Klotz
Martin G
MG
D000641:Ammonia / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002841:Chromatiaceae / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D008969:Molecular Sequence Data; D005810:Multigene Family; D010088:Oxidoreductases / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D024363:Transcription Initiation Site
D001426:Bacterial Proteins; D000641:Ammonia; D010088:Oxidoreductases; C045582:ammonia monooxygenase