New components of Yarrowia lipolytica Golgi multi-protein complexes containing the alpha-1,6-mannosyltransferases YlMnn9p and YlAnl1p.
This paper reports the identification and the characterization of two new components of Yarrowia lipolytica Golgi multi-protein complexes. Blast analysis on the Y. lipolytica complete genome allowed us to find a new alpha-1,6-mannosyltransferase, YlAnl2p, which displays an overall identity of 59% and shares a Golgi cellular localization with the previously described YlAnl1p. Moreover, YlAnl2p was shown to directly interact with YlMnn9p using the two-hybrid system suggesting that the two proteins form a second Golgi sub-complex. In order to further elucidate the composition of the Y. lipolytica Golgi complexes containing alpha-1,6-mannosyltransferases, as M-Pol complexes in Saccharomyces cerevisiae, two-hybrid screens were performed using either YlMnn9p or YlAnl1p as bait. A specific partner of YlAnl1p, named YlAni1p was identified. The two proteins were shown to co-localize and co-precipitate in Y. lipolytica. YlAni1p, which displays a coiled-coil domain as Golgin, and YlAnl1p could be involved in the Golgi apparatus maintenance in the yeast Y. lipolytica.
Barnay-Verdier
Stéphanie
S
Laboratoire de Microbiologie et Génétique Moléculaire, CNRS-Institut National Agronomique Paris-Grignon, INRA, Thiverval-Grignon, France. stephanie.barnay@crc.jussieu.fr
Beckerich
Jean-Marie
JM
Boisramé
Anita
A
D000595:Amino Acid Sequence; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000201:enzymology* / Q000378:metabolism; D008364:Mannosyltransferases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques; D025062:Yarrowia / Q000201:enzymology* / Q000235:genetics
D005656:Fungal Proteins; D009097:Multienzyme Complexes; D008364:Mannosyltransferases; C058478:alpha 1,6-mannosyltransferase