Induction of IL-8 in periodontal ligament cells by H(2)O (2).
Periodontitis is an inflammatory disease caused by bacteria. In periodontitis, reactive oxygen species (ROS) are released from inflammatory cells in response to bacteria. Interleukin (IL)-8 is one of pro-inflammatory cytokines. To investigate the role of ROS in pathogenesis of periodontitis, we estimated the effect of H(2)O(2), one of ROS, on the expression of IL-8 in human periodontal ligament (PDL) cells. PDL cells were treated with H(2)O(2). IL-8 expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38) and c-jun NH(2)-terminal kinase (JNK) was estimated by Western blotting. Treatment with H(2)O(2) at concentration of up to 250 microM increased IL-8 mRNA expression and production in a concentration-dependent manner. However, treatment with 500 microM H(2)O(2) did not increase IL-8 production. Catalase, an inhibitor of H(2)O(2), down-regulated the production of IL-8 induced by H(2)O(2). H(2)O(2) increased the phosphorylation of ERK, p38, and JNK. Pretreatment with PD98059 (ERK inhibitor), SB203580 (p38 inhibitor), or SP600125 (JNK inhibitor) decreased the IL-8 production induced by H(2)O(2). These results indicate that H(2)O(2) acts as an inducer of IL-8 secretion via activation of ERK, p38, and JNK in PDL cells. H(2)O(2) deposited in periodontal tissue during inflammation against bacteria may accelerate tissue destruction via induction of IL-8 in PDL cells.
Lee
Yang-Sin
YS
Department of Oral Biology, BK21 project, Oral Science Research Center, Yonsei University College of Dentistry, Seoul 120-752, Republic of Korea.
Bak
Eun Jung
EJ
Kim
Minyoung
M
Park
Wonse
W
Seo
Jeong Taeg
JT
Yoo
Yun-Jung
YJ
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D010513:Periodontal Ligament / Q000187:drug effects* / Q000276:immunology*; D010518:Periodontitis / Q000276:immunology* / Q000382:microbiology; D010766:Phosphorylation; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects*
D016209:Interleukin-8; D006861:Hydrogen Peroxide; D020928:Mitogen-Activated Protein Kinases