title: DNA adenine methylation of sams1 gene in symbiont-bearing Amoeba proteus.
abstract: The expression of amoeba sams genes is switched from sams1 to sams2 when amoebae are infected with Legionella jeonii. To elucidate the mechanism for the inactivation of host sams1 gene by endosymbiotic bacteria, methylation states of the sams1 gene of D and xD amoebae was compared in this study. The sams1 gene of amoebae was methylated at an internal adenine residue of GATC site in symbiont-bearing xD amoebae but not in symbiont-free D amoebae, suggesting that the modification might have caused the inactivation of sams1 in xD amoebae. The sams1 gene of xD amoebae was inactivated at the transcriptional level. Analysis of DNA showed that adenine residues in L. jeonii sams were also methylated, implying that L. jeonii bacteria belong to a Dam methylase-positive strain. In addition, both SAM and Met appeared to act as negative regulators for the expression of sams1 whereas the expression of sams2 was not affected in amoebae.
authors.affiliation: Department of Biology, College of Natural Sciences, Chosun University, Gwangju 501-759, Republic of Korea. tjeon@chosun.ac.kr
authors.forename: Taeck J
authors.initials: TJ
authors.lastname: Jeon
mesh_terms: D000656:Amoeba / Q000201:enzymology* / Q000235:genetics* / Q000382:microbiology / Q000502:physiology; D000818:Animals; D019175:DNA Methylation; D005786:Gene Expression Regulation; D020868:Gene Silencing; D007875:Legionella / Q000502:physiology*; D008716:Methionine Adenosyltransferase / Q000235:genetics* / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D013559:Symbiosis
chemical_list: D015800:Protozoan Proteins; D008716:Methionine Adenosyltransferase