Gene expression analysis of neural cells and tissues using DNA microarrays.
DNA microarrays pose specific challenges to those studying the central and peripheral nervous systems. Probably the most important involve difficulty in obtaining appropriate tissue for study, as well as the problems posed by cellular heterogeneity. This unit describes advances in the available technologies and provides protocols for cDNA microarray hybridization, including the use of PCR amplicons. Protocols are also provided for the two major methods for limiting cellular heterogeneity by study of RNA from single cell populations in high-throughput microarray studies, laser capture microdissection (LCM), and automated fluorescent cell sorting (FACS-array).
Karsten
Stanislav L
SL
David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Kudo
Lili C
LC
Geschwind
Daniel H
DH
D000818:Animals; D005434:Flow Cytometry / Q000379:methods / Q000592:standards; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D006801:Humans; D042282:Microdissection / Q000379:methods / Q000592:standards; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D009420:Nervous System / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger