title: Metagenomic diagnosis of bacterial infections.
abstract: To test the ability of high-throughput DNA sequencing to detect bacterial pathogens, we used it on DNA from a patient's feces during and after diarrheal illness. Sequences showing best matches for Campylobacter jejuni were detected only in the illness sample. Various bacteria may be detectable with this metagenomic approach.
authors.affiliation: International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Nakamura
authors.forename: Norihiro
authors.initials: N
authors.lastname: Maeda
authors.forename: Ionut Mihai
authors.initials: IM
authors.lastname: Miron
authors.forename: Myonsun
authors.initials: M
authors.lastname: Yoh
authors.forename: Kaori
authors.initials: K
authors.lastname: Izutsu
authors.forename: Chidoh
authors.initials: C
authors.lastname: Kataoka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Honda
authors.forename: Teruo
authors.initials: T
authors.lastname: Yasunaga
authors.forename: Takaaki
authors.initials: T
authors.lastname: Nakaya
authors.forename: Jun
authors.initials: J
authors.lastname: Kawai
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Horii
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Iida
mesh_terms: D000328:Adult; D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods; D002169:Campylobacter Infections / Q000150:complications / Q000175:diagnosis; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D003937:Diagnosis, Differential; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic