title: Organic-inorganic matrix for electrochemical immunoassay: Detection of human IgG based on ZnO/chitosan composite.
abstract: A new strategy to construct amperometric immunosensor for human IgG assay based on ZnO/chitosan composite as sensing platform has been described. This material, which combined the advantages of inorganic species, ZnO and organic polymer, chitosan, can maintain biological activity well. A sequential sandwich immunoassay format was performed on the ZnO/chitosan composite supported by glass carbon electrode (GCE) using goat-anti-human IgG antibody (IgG Ab) and human IgG as a model system. Amperometry was used to determine the amount of horse-radish peroxidase (HRP) fixed on the sensor surface, which was related to the content of the desired human IgG. Assay conditions that were optimized included the amount of labeled antibody, the incubation time and temperature, the pH of the substrate solution, etc. Using hydroquinone as a mediator, amperometric detection at -150mV (versus SCE) resulted in a detection range 2.5-500ngmL(-1), with a detection limit of 1.2ngmL(-1). The simple manipulations of the construction of ZnO/chitosan composite, as well as low-cost and broad linear range, are the main features of the proposed immunosensing method.
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Cheometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Wang
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Yang
authors.forename: Jishan
authors.initials: J
authors.lastname: Li
authors.forename: Jilai
authors.initials: J
authors.lastname: Gong
authors.forename: Guoli
authors.initials: G
authors.lastname: Shen
authors.forename: Ruqin
authors.initials: R
authors.lastname: Yu