Simultaneous LIF and retro-reflected beam interference detection for CE.
This Short Communication describes a novel optical detection method for CE, based on the combination of LIF detection and retro-reflected beam interference detection. By the use of a side-illuminated laser beam, an on-column multifunctional detection for CE has been developed, and some key elements in the scheme have been optimized. In addition, two miscellaneous samples including fluorescent dyes, carbohydrates and amino acids have been determined to evaluate its performance. Without any additional pretreatment, sufficient LOD and linear ranges have been achieved for most analytes. Its performance in retro-reflected beam interference detection is better than those mentioned in a former report, and its fluorescent sensitivity is comparable with the achievable sensitivity by conventional LIF systems, all of which together combine high sensitivity and universal analysis to a certain extent.
Xiong
Bo
B
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, PR China.
Wang
Wei
W
Miao
Xueyan
X
Zhou
Xiaodong
X
Deng
Yanzhuo
Y
Zhou
Ping
P
Hu
Jiming
J
D000409:Alanine / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D007834:Lasers; D016014:Linear Models; D012680:Sensitivity and Specificity
D002241:Carbohydrates; D005456:Fluorescent Dyes; D000409:Alanine