title: Characterization and expansion of mesenchymal progenitor cells from first-trimester chorionic villi of human placenta.
abstract: BACKGROUND
  Mesenchymal stromal cells (MSC) have been identified in a variety of fetal and adult tissues, including bone marrow (BM), fetal blood and liver. We report on the isolation, expansion and differentiation in vitro of MSC-like cells from chorionic villi (CV).
  
  
  METHODS
  We evaluated 10 samples of CV collected at the first trimester (gestational age 11-13 weeks). We only used cells taken from back-up culture after a successful karyotype analysis. CV cells were characterized by morphologic, immunophenotypic and molecular analysis. The differentiation ability of mesenchymal and neural lineages was detected using specific culture conditions. Cell expansion was assessed after plating cells at different densities in different media, supplemented with animal and human serum.
  
  
  RESULTS
  CV cells showed a homogeneous population of spindle-shaped cells after the first passage. Cells expressed CD90, CD105, CD73, CD44, CD29 and CD13 but not CD45, CD14, CD34 and CD117. They expressed Oct-4, Rex-1, GATA-4 and nestin, which characterize the undifferentiated stem cell state. They differentiated into osteocytes, adipocytes, chondrocytes and neuronal cells. Cell expansion was greater than that of adult BM-derived MSC, 9 logs with fetal bovine serum and 6 logs with human serum. Despite their high proliferative capacity, we did not observe any karyotypic abnormalities after culture.
  
  
  CONCLUSIONS
  Our study shows that CV cells have better potential for expansion than adult stem cells. They can proliferate in a medium with human allogeneic serum and can differentiate into mesenchymal and neural lineages. CV cells may be an excellent cell source for therapeutic applications.
authors.affiliation: Clinica di Ematologia, Dipartimento Scienze Mediche e Chirurgiche, Universita Politecnica delle Marche, Ancona, Italy. a.poloni@univpm.it
authors.forename: A
authors.initials: A
authors.lastname: Poloni
authors.forename: V
authors.initials: V
authors.lastname: Rosini
authors.forename: E
authors.initials: E
authors.lastname: Mondini
authors.forename: G
authors.initials: G
authors.lastname: Maurizi
authors.forename: S
authors.initials: S
authors.lastname: Mancini
authors.forename: G
authors.initials: G
authors.lastname: Discepoli
authors.forename: S
authors.initials: S
authors.lastname: Biasio
authors.forename: G
authors.initials: G
authors.lastname: Battaglini
authors.forename: E
authors.initials: E
authors.lastname: Berardinelli
authors.forename: F
authors.initials: F
authors.lastname: Serrani
authors.forename: P
authors.initials: P
authors.lastname: Leoni
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D011247:Pregnancy; D011261:Pregnancy Trimester, First
chemical_list: D015415:Biomarkers