title: Flow-injection determination of trace amounts of dopamine by chemiluminescence detection.
abstract: A flow-injection analysis (FIA) for the determination of dopamine has been developed. The method is based on the inhibition effect of dopamine on the iron(II)-induced chemiluminescence (CL) of 10,10'-dimethyl-9,9'-biacridinium dinitrate (lucigenin). The presence of a non-ionic surfactant, polyoxyethylene (23) lauryl ether (Brij 35), caused an increase in the inhibition effect. The present method allows the determination of dopamine over the range 1x10(-8)-2x10(-7) mol dm(-3). The relative standard deviation was 0.7% for eight determinations of 6x10(-8) mol dm(-3) dopamine. The detection limit (S/N=3) was 2x10(-9) mol dm(-3) with the sampling rate of 40 samples h(-1). The effect of other catecholamines and compounds of similar structure on the lucigenin CL reaction was studied: quinone, hydroquinone, norepinephrine, pyrocatechol and l-dopa suppressed the CL intensity.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Tsukuba, Tsukuba 305-8571, Japan.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: N
authors.initials: N
authors.lastname: Teshima
authors.forename: T
authors.initials: T
authors.lastname: Hasebe
authors.forename: M
authors.initials: M
authors.lastname: Kurihara
authors.forename: T
authors.initials: T
authors.lastname: Kawashima