Beta-catenin overexpression in Dupuytren's disease is unrelated to disease recurrence.
Recurrence of Dupuytren's contracture is common yet unpredictable, compromising surgical outcome. The alpha-smooth muscle actin-containing myofibroblast is the active contractile cellular component. Based on recent reports on beta-catenin accumulation in Dupuytren's disease, we investigated a possible relation with disease recurrence. We divided a collection of 143 nodules into those from patients with recurrent or nonrecurrent nodules and with a minimal 3-year followup. We randomly selected 12 and 11 samples of each group, respectively. We looked at Dupuytren's diathesis, immunohistologic staining for beta-catenin and alpha-smooth muscle actin, and Luck's histologic stages (zones). The expression of selected Wnt genes was examined with TaqMan PCR in separate histologic zones. All samples showed cytoplasmic and nuclear beta-catenin accumulation in myofibroblasts in involutional zones. The risk score of Abe et al. and Dupuytren's diathesis were greater in the recurrent group. Greater Wnt5a expression in the beta-catenin-accumulating involutional zone was seen. We conclude intracellular beta-catenin accumulation, possibly regulated by upstream Wnt signaling pathway activation and confined in myofibroblasts in the involutional zone of Dupuytren's diathesis, is unrelated to disease recurrence. Clinical parameters for Dupuytren's diathesis remain the best way to predict recurrence risk.
Degreef
Ilse
I
Orthopaedic Surgery Department, Hand Unit, University Hospitals Leuven, Pellenberg Campus, Weligerveld 1, 3212, Pellenberg, Belgium. ilse.degreef@uz.kuleuven.ac.be
De Smet
Luc
L
Sciot
Raf
R
Cassiman
Jean-Jacques
JJ
Tejpar
Sabine
S
D000199:Actins / Q000032:analysis; D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D004387:Dupuytren Contracture / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005260:Female; D005347:Fibroblasts / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012008:Recurrence; D018570:Risk Assessment; D012307:Risk Factors; D016896:Treatment Outcome; D015854:Up-Regulation; D051153:Wnt Proteins / Q000032:analysis / Q000235:genetics; D055815:Young Adult; D051176:beta Catenin / Q000032:analysis*
D000199:Actins; C495270:CTNNB1 protein, human; D012333:RNA, Messenger; D051153:Wnt Proteins; D051176:beta Catenin