Establishment and characterization of hepatic stem-like cell lines from normal adult rat liver.
The liver is a unique organ with the potential to regenerate from injury. Hepatic stem cells contribute to liver regeneration when surviving hepatocytes in injured liver are unable to proliferate. To investigate the mechanism of liver regeneration in vitro, we established hepatic stem cell lines named HY1, HY2 and HY3, derived from a healthy liver of adult rat. HY cells showed an expression pattern similar to oval cells, and efficiently induced hepatic differentiation following sequential treatment with type I collagen, transforming growth factor-beta1 (TGF-beta1), and hepatocyte growth factor (HGF) or oncostatin M (OSM). These results suggested that HY cells are liver stem cells representing an excellent tool for in vitro studies on liver regeneration.
Hirata
Mitsuhi
M
Division of Molecular Biology, School of Life Science, Tottori University Faculty of Medicine, 86 Nishicho, Yonago 683-8503, Japan.
Amano
Kumiko
K
Miyashita
Ayumi
A
Yasunaga
Mayu
M
Nakanishi
Tomoko
T
Sato
Kenzo
K
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D053773:Transforming Growth Factor beta1; D017228:Hepatocyte Growth Factor