title: Immunochemical composition of cryoglobulins generated in stroke.
abstract: BACKGROUND
  Cryoglobulins are abnormal immune complexes where both the antigens and the antibodies are immunoglobulins. The ability of cryoglobulins to bind C-reactive protein and low density lipoproteins, activate complement, and stimulate production of tumor necrosis factor-alpha generates interest in studying cryoglobulins in ischemic stroke.
  
  
  METHODS
  We determined blood levels of cryoglobulins in patients with ischemic stroke at different time points of stroke onset and identified the composition of cryoglobulins isolated from the blood on the first day of stroke onset.
  
  
  RESULTS
  On days 1-14, significantly elevated levels of cryoglobulins were detected with the maximum level on day 3.
  
  
  CONCLUSIONS
  Determination of immunoglobulin (Ig) content of cryoglobulins revealed the presence of a mixture of polyclonal IgG, IgA, and IgM, C3 complement protein and its activation split products, C1q complement protein, pathogenic lipoprotein-X, and beta-lipoprotein.
  
  
  CONCLUSIONS
  We suggest that cryoglobulins are involved in post-ischemic inflammatory response through activation of the complement cascade and cytokines production.
authors.affiliation: Institute of Molecular Biology, National Academy of Sciences of Armenia, 7 Hasratyan St., Yerevan 0014, Armenia.
authors.forename: Levon
authors.initials: L
authors.lastname: Manukyan
authors.forename: Anna
authors.initials: A
authors.lastname: Boyajyan
authors.forename: Arsen
authors.initials: A
authors.lastname: Arakelyan
authors.forename: Violetta
authors.initials: V
authors.lastname: Ayvazyan
authors.forename: Elina
authors.initials: E
authors.lastname: Arakelova
authors.forename: Robert
authors.initials: R
authors.lastname: Sim
authors.forename: George
authors.initials: G
authors.lastname: Grigoryan
mesh_terms: D000368:Aged; D000936:Antigen-Antibody Complex / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D015922:Complement C1q / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D003176:Complement C3 / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D003450:Cryoglobulins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007120:Immunochemistry; D008073:Lipoprotein-X / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015427:Reperfusion Injury / Q000276:immunology / Q000378:metabolism; D020521:Stroke / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors
chemical_list: D000936:Antigen-Antibody Complex; D003176:Complement C3; D003450:Cryoglobulins; D008073:Lipoprotein-X; D008077:Lipoproteins, LDL; D015922:Complement C1q