Immunoadjuvant effects of bacterial genomic DNA and CpG oligodeoxynucleotides on avian influenza virus subtype H5N1 inactivated oil emulsion vaccine in chicken.
This study investigated the immunoadjuvant effects of three types of bacterial genomic DNA and CpG oligonucleotides (CpG ODN) on the avian influenza virus (AIV) subtype H5N1 inactivated oil emulsion vaccine under two immunization strategies. The genomic DNA extracted from Escherichia coli O(2), Staphylococcus aureus,Streptococcus faecalis FQ68, and synthetic CpG ODN were used as adjuvants, and their effects on the AIV oil emulsion vaccine were examined in chickens. The results indicated that when administered separately from the vaccine, adjuvants induced lower haemagglutination inhibition (HI) titres and serum IgG titres but resulted in higher concentrations of IFN-gamma and IL-10. In contrast, when combined with the oil emulsion vaccine prior to inoculation, CpG ODN induced higher HI, IgG titres and IFN-gamma concentration but resulted in lower IL-10 concentration. These data suggest that, depending on the immunization approaches, adjuvants may exert distinct immune effects in chickens receiving AIV H5N1 oil emulsion vaccine: the prior incorporation of CpG ODN into the vaccine may augment both the humoral and Th1 type immune responses, while separate inoculation of adjuvants has not shown better adjuvanticity.
Wang
Yimeng
Y
School of Biological and Food Engineering, Dalian Polytechnic University, Dalian 116034, PR China. ammenwang@gmail.com
Shan
Chunqiao
C
Ming
Shuangxi
S
Liu
Yan
Y
Du
Yuchun
Y
Jiang
Guotuo
G
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D000917:Antibody Formation; D001419:Bacteria / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002645:Chickens / Q000276:immunology*; D004269:DNA, Bacterial / Q000276:immunology*; D004655:Emulsions / Q000627:therapeutic use; D013293:Enterococcus faecalis / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D005798:Genes, Bacterial; D016680:Genome, Bacterial / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology; D005585:Influenza in Birds / Q000276:immunology*; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D009838:Oligodeoxyribonucleotides / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D004269:DNA, Bacterial; D004655:Emulsions; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides; D014765:Viral Vaccines; D016753:Interleukin-10; D007371:Interferon-gamma