Biosensing based on NADH detection coupled to electrogenerated chemiluminescence from ruthenium tris(2,2'-bipyridine).
A flow injection analysis system for a reduced form of nicotinamide adenine dinucleotide (NADH) was constructed by the electrogenerated chemiluminescence using ruthenium tris(2,2'-bipyridine) complex, and this technique was applied to the determination of ethanol concentration by using an immobilized alcohol dehydrogenase (ADH). The NADH determination was optimum under the following conditions. The flow rate was 1.5 to 2.0 ml/min, and the applied voltage was 1.6 V. The NADH calibration plot showed the linear correlation in the concentration range 10 to 250muM, and the lower detection limit was 10muM. Ethanol was successfully measured in the concentration range 10 ppm to 5% by using ADH-immobilized glass beads.
Yokoyama
K
K
School of Materials Science, Japan Advanced Institute of Science and Technology, Hokuriku, 15 Asahidai, Tatsunokuchi-machi, Nomigun, Ishikawa 923-12, Japan.
Sasaki
S
S
Ikebukuro
K
K
Takeuchi
T
T
Karube
I
I
Tokitsu
Y
Y
Masuda
Y
Y