Preparative argentation reversed-phase high performance liquid chromatography.
In this study, a preparative chromatography method named preparative argentation reversed-phase high performance liquid chromatography (Ag-RP-HPLC) was developed by adding silver ion to the mobile phase of preparative HPLC. Firstly, an analytical Ag-RP-HPLC method was developed, and the effects of silver content and acid content in the mobile phase on the column efficiency were studied. Based on the method of linear amplification, a preparative Ag-RP-HPLC technique with optimized separation conditions was developed. The new technique was applied successfully to the separation of the unsaturated aliphatic acid amide isomers contained in Asarum forbesii Maxim. Compared with the commonly used technique of argentation normal phase chromatography, this method with little solvent consumption is simple, fast, efficient, and flexible for the isolation and purification of the unsaturated compounds.
Zhang
Feng
F
Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
Chu
Xiaogang
X
Wang
Xiujuan
X
Yang
Minli
M
Ling
Yun
Y
Sun
Li
L
Li
Xiuqin
X
Yong
Wei
W
D000996:Antitussive Agents / Q000302:isolation & purification; D030023:Asarum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D053284:Polyunsaturated Alkamides / Q000302:isolation & purification; D012834:Silver / Q000737:chemistry*; D013237:Stereoisomerism
C452804:2,4,8,10-dodecatetraenoic acid isobutylamide; D000996:Antitussive Agents; D005231:Fatty Acids, Unsaturated; D053284:Polyunsaturated Alkamides; D012834:Silver