title: Flow-injection chemiluminescence determination of epinephrine in pharmaceutical preparations using raw apple juice as enzyme source.
abstract: Raw apple juice exhibits the activity of polyphenol oxidase which can be employed for the determination of mono-, di- and polyhydric phenols. The chemiluminescence detection in the two-channel manifold was used to develop the new procedure for determination of epinephrine. Epinephrine can be determined by this method in pharmaceutical preparations in concentration ranges 1.0-10.0 and 10.0-25.0 mg l(-1), with a detection limit of 0.2 mg l(-1). Good selectivity against typical antioxidants and other coexisting substances was achieved. Relatively slow loss of the enzyme activity was observed during 1 week. The proposed method is very simple. Fresh juice solution from one apple can be prepared in 10 min and used for at least 8 h with excellent precision. Consumption of luminol solution was 0.15 ml min(-1). The throughput was 20 samples per hour.
authors.affiliation: Institute of Chemistry, University of Białystok, al. J. Pilsudskiego 11/4, 15-443 Białystok, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Michałowski
authors.forename: P
authors.initials: P
authors.lastname: Hałaburda