title: Micellar electrokinetic capillary chromatography as an alternative method for the determination of ethinylestradiol and levo-norgestrel.
abstract: A method for quantifying of ethinylestradiol (ETE) and levo-norgestrel (LEV) in pharmaceutical products by micellar electrokinetic chromatography (MEKC) is described. The separation was carried out at 25 degrees C and 25 kV, using a 20 mM borate buffer (pH 9.2), 15 mM sodium dodecylsulfate (SDS) in 30% acetonitrile/water (v/v). Under these conditions the analysis takes about 7 min. The method has been applied for quantifying both compounds in six different commercial contraceptives and the proposed method gave good results when compared with a reference liquid chromatographic (LC) method.
authors.affiliation: Departamento de Quimica Analitica y Tecnologia de Alimentos, Facultad de Quimicas, Universidad de Castilla-La Mancha, E-13071 Ciudad Real, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Berzas
authors.forename: B
authors.initials: B
authors.lastname: Del Castillo
authors.forename: G
authors.initials: G
authors.lastname: Castañeda
authors.forename: M J
authors.initials: MJ
authors.lastname: Pinilla