Urea solid-state biosensor suitable for continuous dialysis control.
A solid state potentiometric biosensor for urea determination was assembled coupling a nonactin-based ISE without an internal solution with immobilized urease. The system proved to be useful for continuous monitoring of urea during dialysis treatment using the ultrafiltrate of blood as sample. A noticeable shift of potential occurred during the dialysis (probably due to continuous extraction of ionophore by the ultrafiltrate). Therefore a flow injection analysis assembly was necessary to control the shift of electrode potential.
Zamponi
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Dipartimento de Sanita Pubblica, Epidemiologia e Chimica Analitica Ambientale, Sezione di Chimica Analitica, Via Gino Capponi 9, 50121 Firenze, Italy.
Lo Cicero
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Mascini
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Della Ciana
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Sacco
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