Nucleotide sequence and genome organization of a member of a new and distinct Caulimovirus species from dahlia.
A distinct caulimovirus, associated with dahlia mosaic, was cloned and sequenced. The caulimovirus, tentatively designated as dahlia common mosaic virus (DCMV), had a double-stranded DNA genome of ca. 8 kb. The genome organization of DCMV was found to be typical of members of the genus Caulimovirus and consisted of six major open reading frames (ORFs), ORFs I-VI, and one minor ORF, ORF VII. Sequence comparisons with the DNA genomes of two known caulimoviruses isolated from dahlia, Dahlia mosaic virus (DMV) and an endogenous caulimovirus, DMV-D10, showed that DCMV is a member of a distinct caulimovirus species, with sequence identities among various ORFs ranging from 25 to 80%.
Pappu
H R
HR
Department of Plant Pathology, Washington State University, PO Box 646430, Pullman, WA 99164-6430, USA. hrp@wsu.edu
Druffel
K L
KL
Miglino
R
R
van Schadewijk
A R
AR
D001483:Base Sequence; D017796:Caulimovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D031944:Dahlia / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*