title: Voltammetric determination of 5-hydroxydole-3-acetic acid in human gastric juice.
abstract: A method for the determination of the major serotonin metabolite--5-hydroxyindole-3-acetic acid (5-HIAA) in human gastric juice by cyclic voltammetry was described. The measurement conditions were investigated. The potential window was chosen from +0.1 to +0.9 V, the supporting electrolyte was 0.025 M PBS solution (pH 2.0). The method allowed determination in the concentration range from 2.0 x 10(-7) to 2.0 x 10(-5) M and a detection limit of 80 nM. When samples of gastric juice were analyzed with the method, we obtained the mean content of 5-HIAA in the gastric juice. Meanwhile, interference from other ions and substances were examined. The experimental results indicate that the method for the determination of gastric juice samples is successful.
authors.affiliation: Department of Chemistry, Jilin University, Changchun, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: W
authors.initials: W
authors.lastname: Song
authors.forename: N
authors.initials: N
authors.lastname: Lu
authors.forename: M
authors.initials: M
authors.lastname: Zou
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.forename: Z
authors.initials: Z
authors.lastname: Yu