title: Temporal proteomic analysis of intestine developing necrotizing enterocolitis following enteral formula feeding to preterm pigs.
abstract: Necrotizing enterocolitis (NEC), a serious gastrointestinal inflammatory disease, frequently occurs in preterm neonates that fail to adapt to enteral nutrition. A temporal gel-based proteomics study was performed on porcine intestine with NEC lesions induced by enteral formula feeding. Functional assignment of the differentially expressed proteins revealed that important cellular functions, such as the heat shock response, protein processing; and purine, nitrogen, energy metabolism, were possible involved in the early progression of NEC.
authors.affiliation: Division of Agricultural, Food and Nutritional Science, School of Biological Sciences, The University of Hong Kong, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Hang
authors.forename: Per T
authors.initials: PT
authors.lastname: Sangild
authors.forename: Wai-Hung
authors.initials: WH
authors.lastname: Sit
authors.forename: Heidi Hoi-Yee
authors.initials: HH
authors.lastname: Ngai
authors.forename: Ruojun
authors.initials: R
authors.lastname: Xu
authors.forename: Jayda Lee Ann
authors.initials: JL
authors.lastname: Siggers
authors.forename: Jennifer Man-Fan
authors.initials: JM
authors.lastname: Wan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D004750:Enteral Nutrition / Q000009:adverse effects*; D020345:Enterocolitis, Necrotizing / Q000175:diagnosis* / Q000378:metabolism* / Q000662:veterinary; D020869:Gene Expression Profiling / Q000379:methods; D007091:Image Processing, Computer-Assisted; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000473:pathology; D013058:Mass Spectrometry / Q000379:methods; D018529:Nutritional Support / Q000009:adverse effects*; D047928:Premature Birth; D040901:Proteomics / Q000379:methods*; D013552:Swine; D013997:Time Factors