Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC.
Since the discovery of multiple bioactivities for agarobiose oligomers, a quantitative method has been in great need to monitor the agarobiose oligomers. This report demonstrates that agarobiose oligomers can be separated with high resolution in HPLC after introducing alpha-naphthylamine into compounds. Agarobiose oligomers ranged from biose to decaose were isolated by Sephadex column. HPLC analysis indicated that each oligomer could be quantified with good linearity and a low detection limit of 0.1-4mug/ml. The chromatographic profiles of agaro-oligosaccharides with different hydrolysis modes (hydrochloride, citric acid, solid acid, and hydroxyl radical degradation) showed that agarobiose could be obtained more than 57.8% using solid acid mediated hydrolysis, while hydrochloride acid could degrade agar into a series of agaro-oligosaccharides from biose to decaose. The yield of oligosaccharides was low if hydrolyzed by citric acid. The Fenton degradation can increase the speed of hydrolysis, but the product was complex.
Chen
Hai-Min
HM
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China; The Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China.
Zheng
Li
L
Lin
Wei
W
Yan
Xiao-Jun
XJ