Spectrophotometric method for determination of vanadium and its application to industrial, environmental, biological and soil samples.
The very sensitive, fairly selective direct spectrophotometric method for the determination of trace amount of vanadium (V) with 1,5-diphenylcarbohydrazide (1,5-diphenylcarbazide) has been developed. 1,5-diphenylcarbohydrazide (DPCH) reacts in slightly acidic (0.0001-0.001 M H(2)SO(4) or pH 4.0-5.5) 50% acetonic media with vanadium (V) to give a red-violet chelate which has an absorption maximum at 531 nm. The average molar absorption coefficient and Sandell's sensitivity were found to be 4.23 x 10(4) l mol(-1) cm(-1) and 10 ng cm(-2) of V(v), respectively. Linear calibration graph were obtained for 0.1-30 microg ml(-1) of V(v): the stoichiometric composition of the chelate is 1:3 (V: DPCH). The reaction is instantaneous and absorbance remain stable for 48 h. The interference from over 50 cations, anions and complexing agents has been studied at 1 microg ml(-1) of V(v). The method was successfully used in the determination of vanadium in several standard reference materials (alloys and steels), environmental waters (potable and polluted), biological samples (human blood and urine), soil samples, solution containing both vanadium (V) and vanadium (IV) and complex synthetic mixtures. The method has high precision and accuracy (s=+/-0.01 for 0.5 microg ml(-1)).
Ahmed
M J
MJ
Laboratory of Analytical Chemistry, Department of Chemistry, University of Chittagong, Chittagong, Bangladesh. vc-cu@spnetctg.com
Banoo
S
S