Abortiporus biennis tolerance to insoluble metal oxides: oxalate secretion, oxalate oxidase activity, and mycelial morphology.
The ability of Abortiporus biennis to tolerate and solubilize toxic metal oxides (Cu(2)O, Al(2)O(3), ZnO, CuFe(2)O(4)Zn, CdO, and MnO(2)) incorporated into agar media was investigated and the growth rate, oxalic acid secretion, and mycelial morphology were monitored. Among the tested metal oxides, formation of clear zones underneath the mycelium growing on Cu(2)O- and ZnO-amended plates was observed. ZnO, CdO and Cu(2)O caused the highest rate of fungal growth inhibition. An increased level of oxalic acid concentration was detected as a response of A. biennis to the presence of Cu(2)O, MnO(2), ZnO and CuFe(2)O(4)Zn in growth medium. The oxalate oxidase (OXO) was found to be responsible for oxalic acid degradation in A. biennis cultivated in metal-amended media. An increased level of OXO was observed in media amended with Cu(2)O, ZnO and MnO(2). Confocal microscopy used in this study revealed changes in mycelial morphology which appeared as increased hyphal branching, increased septation and increased spore number.
Graz
Marcin
M
Department of Biochemistry, Maria Curie-Sklodowska University, Sklodowska Place 3, 20-031 Lublin, Poland. graz@poczta.umcs.lublin.pl
Jarosz-Wilkołazka
Anna
A
Pawlikowska-Pawlega
Bozena
B
D000537:Aluminum Oxide / Q000494:pharmacology; D001487:Basidiomycota / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D019187:Cadmium Compounds / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D017895:Manganese Compounds / Q000494:pharmacology; D018613:Microscopy, Confocal; D025282:Mycelium / Q000166:cytology* / Q000187:drug effects; D019815:Oxalic Acid / Q000378:metabolism*; D010087:Oxides / Q000494:pharmacology*; D010088:Oxidoreductases / Q000378:metabolism*; D015034:Zinc Oxide / Q000494:pharmacology
D019187:Cadmium Compounds; D017895:Manganese Compounds; D010087:Oxides; C029663:cadmium oxide; D003300:Copper; D019815:Oxalic Acid; D010088:Oxidoreductases; C020598:oxalate oxidase; D000537:Aluminum Oxide; D015034:Zinc Oxide; C000520:cuprous oxide; C016552:manganese dioxide