High performance liquid chromatographic determination of copper(II) and nickel(II) by using solvent extraction and bis(acetylpivalylmethane)ethylenediimine as complexing reagent.
The reagent bis(acetylpivalylmethane)ethylenediimine has been examined for the HPLC separation of copper(II), nickel(II), palladium(II) and oxovanadium(IV) chelates on reversed phase HPLC columns (250 x 4 mm) packed with Hypersil ODS, 5 mu and (150 x 3.9 mm) Nova Pak C(18) with guard column. The complexes are eluted with a binary mixture of methanol and water or methanol, acetonitrile and water. Detection is achieved with a UV detector. The solvent extraction procedure is used for the determination of copper and nickel simultaneously at microgram levels, corresponding to ng levels and pg levels respectively, per injection. The method has been applied to the determination of copper and nickel in a coin, nickel-aluminum alloy and water samples.
Khuhawar
M Y
MY
Institute of Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan.
Soomro
A I
AI