Micellar modification of the spectral, intensity and lifetime characteristics of the fluorescence of fluorescein-labelled phenobarbital.
The effects of association with micelles on the fluorescence properties of fluorescein-labelled phenobarbital were studied as a function of pH (in the range 5.8-7.8) and micelle concentration. The largest changes were observed at pH 5.8, including red-shifts of 10 nm in the emission and excitation maxima, spectral intensity enhancements as great as 500%, and 0.5-nsec increases in fluorescence lifetime. Micelles induced much greater changes than cyclodextrins, which were also studied. These studies indicate the potential usefulness of micelles and cyclodextrins as auxiliary binding reagents for both heterogeneous and homogeneous fluoroimmunoassay techniques.
Keimig
T L
TL
Department of Chemistry, Oklahoma State University, Stillwater, OK 74078, USA.
McGown
L B
LB