title: Protective effect of geranylgeranylacetone on cisplatin ototoxicity.
abstract: BACKGROUND
  Geranylgeranylacetone (GGA) has the ability to induce heat shock proteins and to protect cells from apoptotic insults. This study aims to investigate whether GGA has a protective effect on cisplatin (CDDP) ototoxicity.
  
  
  METHODS
  The auditory threshold was assessed using the auditory brainstem response test. Hsp70 and C-reactive protein expressions were investigated by Western blot analysis. The amount of hair cells was counted under scanning electron microscopy.
  
  
  RESULTS
  The auditory threshold and the percentage of missing outer hair cells in the CDDP group were significantly higher than in the GGA + CDDP group. C-reactive protein expression was less in the GGA + CDDP group compared with the CDDP group. Hsp70 expression showed an adverse result.
  
  
  CONCLUSIONS
  It was suggested that GGA had a protective effect on CDDP ototoxicity.
authors.affiliation: Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Wen Hua Xi Road 107, Jinan, China.
authors.forename: Hai Ying
authors.initials: HY
authors.lastname: Yin
authors.forename: Xiu Fang
authors.initials: XF
authors.lastname: Ma
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Xia
authors.forename: An Ting
authors.initials: AT
authors.lastname: Xu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D002097:C-Reactive Protein / Q000187:drug effects / Q000378:metabolism; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D004224:Diterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D018840:HSP70 Heat-Shock Proteins / Q000187:drug effects / Q000378:metabolism; D006198:Hair Cells, Auditory / Q000187:drug effects*; D015151:Immunoblotting; D008855:Microscopy, Electron, Scanning
chemical_list: D000970:Antineoplastic Agents; D004224:Diterpenes; D018840:HSP70 Heat-Shock Proteins; D002097:C-Reactive Protein; D002945:Cisplatin; C031049:geranylgeranylacetone