Cell size increased in tissues from transgenic mice overexpressing a cell surface growth-related and cancer-specific hydroquinone oxidase, tNOX, with protein disulfide-thiol interchange activity.
tNOX (ENOX2), a cancer-specific and growth-related cell surface protein with protein disulfide-thiol interchange and hydroquinone (NADH) oxidase activities was overexpressed in a transgenic mouse model. Female transgenic mice grew faster than wild type as did embryonic fibroblast cells prepared from the transgenic mice. The tissue expression of tNOX mRNA was greatest in heart, lung and liver. When these tissues were analyzed for cell size, the cells from the tissues of transgenic animals were, on average, 20% larger in surface area than cells from corresponding wild-type tissues. Also analyzed were cells of intestine, spleen and kidney in which tNOX overexpression was observed but to a lesser extent. Cell size was increased as well with intestine and kidney but less so with spleen. At the end of the study, carcass weights of the transgenic animals were greater than those of wild type. This increase in carcass weight was reflected in an increase in femur weight and thickness in both male and female transgenic mice but not in femur length. Other carcass parameters such as skin weight and body fat or body fluids were unchanged or changes were insufficient to account for the increased carcass weight. The findings are consistent with the property of tNOX observed in studies with cultured cells as contributing to the enlargement phase of cell growth.
Yagiz
Kader
K
Department of Foods and Nutrition, Purdue University, Stone Hall, 700 W. State Street, West Lafayette, Indiana 47907-2059, USA.
Snyder
Paul W
PW
Morré
D James
DJ
Morré
Dorothy M
DM
D000818:Animals; D049109:Cell Proliferation; D048429:Cell Size; D002478:Cells, Cultured; D004220:Disulfides / Q000737:chemistry*; D005260:Female; D006873:Hydroquinones / Q000378:metabolism; D008297:Male; D051379:Mice; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D004220:Disulfides; D006873:Hydroquinones; D012333:RNA, Messenger; D013438:Sulfhydryl Compounds; D010088:Oxidoreductases; D009247:NADH, NADPH Oxidoreductases; C431909:tumor-associated NADH oxidase; C031927:hydroquinone