title: [Determination of three anabolic steroids by liquid chromatography-mass spectrometry].
abstract: A high performance liquid chromatography-electrospray ionization mass spectrometry (LC-ESI/MS) was developed to screen and determine trenbolone, tetrahydrogestrinone and gestrinone in human urine. The urine sample was enzymatically hydrolyzed with beta-glucuronidase, then extracted with methyl tert-butyl ether. Chromatographic separation was performed on a Zorbax SB-C18 column (150 mm x 2.1 mm, 5 microm) with ammonium formate buffer (pH 3.5) and acetonitrile as mobile phase. Using positive electrospray ionization mode, the effect of different parameters from electrospray chamber was investigated. The limits of detection based on signal noise ratio of 3 were between 1 and 5 ng. The method can be applied in screening and confirmation of the anabolic steroids in doping control.
authors.affiliation: China Anti-Doping Agency, General Administration of Sport of China, Beijing 100029, China. qinyang@chinada.cn
authors.forename: Yang
authors.initials: Y
authors.lastname: Qin
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Zhanliang
authors.initials: Z
authors.lastname: Wang
authors.forename: Moutian
authors.initials: M
authors.lastname: Wu
mesh_terms: D045930:Anabolic Agents / Q000737:chemistry / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004300:Doping in Sports; D006801:Humans; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D013256:Steroids / Q000737:chemistry / Q000652:urine*; D016482:Urinalysis / Q000379:methods*
chemical_list: D045930:Anabolic Agents; D013256:Steroids