Electrochemical studies of rutin interacting with hemoglobin and determination of hemoglobin.
Electrochemical investigation of the interaction of rutin (Rt) with hemoglobin (Hb) at a glassy carbon electrode is reported for the first time. With the addition of hemoglobin in a rutin solution, both the reduction and oxidation currents decrease with few changes in the peak potentials. The reaction of rutin with hemoglobin forms a nonelectroactive supramolecular complex Hb-Rt. The equilibrium constant for complex is calculated to be 3.4x10(6). A satisfactory result has been obtained for the determination of hemoglobin in clinical blood samples.
Bao
X
X
Department of Chemistry, Peking University, Beijing 100871, People's Republic of China.
Zhu
Z
Z
Li
N Q
NQ
Chen
J
J