Detection of a novel allergen in raw tomato.
The study reports a case of "pure" tomato allergy in an adult female. The responsible allergen was partially characterized by immunoblot analysis, pepsin digestion, and heating. It had a molecular weight of about 9 kDa and was heat-labile and pepsin-resistant, thus confirming the clinical history. Unfortunately, due to the failure of both 2-dimensional electrophoresis analysis and N-terminal sequencing experiments, it was not possible to characterize the protein further. Based on a comparison with currently known tomato allergens, this seems to be a novel allergen protein.
Asero
R
R
Ambulatorio di Allergologia, Clinica San Carlo, Paderno Dugnano (MI), Italy. r.asero@libero.it
Mistrello
G
G
Roncarolo
D
D
Amato
S
S
Arcidiacono
R
R
Fortunato
D
D
D015746:Abdominal Pain; D052179:Antigens, Plant / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D015153:Blotting, Western; D003967:Diarrhea; D000939:Epitopes; D005260:Female; D005512:Food Hypersensitivity / Q000209:etiology / Q000276:immunology* / Q000503:physiopathology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D018551:Solanum lycopersicum / Q000276:immunology*; D008875:Middle Aged; D010936:Plant Extracts / Q000302:isolation & purification; D014674:Plant Proteins, Dietary / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000150:complications / Q000276:immunology* / Q000503:physiopathology
D052179:Antigens, Plant; D000939:Epitopes; D010936:Plant Extracts; D014674:Plant Proteins, Dietary; D007073:Immunoglobulin E