title: Identification of Candida albicans and Candida glabrata within 1.5 hours directly from positive blood culture bottles with a shortened peptide nucleic acid fluorescence in situ hybridization protocol.
abstract: Candida albicans and Candida glabrata can be identified in blood culture bottles within 2.5 h using peptide nucleic acid fluorescence in situ hybridization. A 1.25-h protocol was compared to the standard with 40 positive (clinical and spiked) blood culture bottles tested in batches of 5. All C. albicans (15) and C. glabrata (16) isolates, alone or mixed, were identified correctly using both protocols, whereas 18 isolates (five other species) were negative by both protocols. This shortened method will significantly reduce the time to identification.
authors.affiliation: Department of Pathology, the Johns Hopkins Medical Institutions, Baltimore, Maryland, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Gherna
authors.forename: William G
authors.initials: WG
authors.lastname: Merz
mesh_terms: D001769:Blood / Q000382:microbiology*; D002176:Candida albicans / Q000302:isolation & purification*; D041221:Candida glabrata / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009172:Mycology / Q000379:methods*; D020135:Peptide Nucleic Acids; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D020135:Peptide Nucleic Acids