title: High-performance liquid chromatographic method for the simultaneous determination of cyclosporine A and its four major metabolites in whole blood.
abstract: This study aimed to develop a simple and efficient optimized high-performance liquid chromatograph (HPLC) method for simultaneous determination of cyclosporine A (CyA) and its major, partly active metabolites AM1, AM9, AM4N, and AM19 in whole blood from transplant patients using cyclosporine D (CyD) as internal standard. The method used a CN analytical column maintained at 60 degrees C with hexan-isopropanol (93:7, v/v) as mobile phase; detection was at 212nm. Linearity for all five compounds was tested in the range of 31-1500ngml(-1) for CyA and of 31-1000ngml(-1) for metabolites. The limit of detection was found to be 15ngml(-1) for all compounds. This modified, inexpensive method is also suitable for measuring cyclosporine A and metabolite concentrations in routine monitoring of patients undergoing treatment with CyA.
authors.affiliation: Institute of Medical and Chemical Laboratory Diagnostics, University Medical Center, Auenbruggerplatz 29, Graz A-8036, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Khoschsorur
authors.forename: W
authors.initials: W
authors.lastname: Erwa
authors.forename: F
authors.initials: F
authors.lastname: Fruehwirth
authors.forename: M
authors.initials: M
authors.lastname: Stettin
authors.forename: A
authors.initials: A
authors.lastname: Meinitzer
authors.forename: G
authors.initials: G
authors.lastname: Hoebarth
authors.forename: S
authors.initials: S
authors.lastname: Zelzer
authors.forename: G
authors.initials: G
authors.lastname: Halwachs-Baumann