title: Low density lipoprotein (LDL)-antioxidant flavonoids from roots of Sophora flavescens.
abstract: Oxidation of low density lipoprotein (LDL) is strongly implicated as a key process in the onset of atherosclerosis. In this study, nine alkylated (C10-C5) flavonoids from Sophora flavescens were examined for their inhibitory effects on copper-induced LDL oxidation. Of the flavonoids tested, sophoraflavanone G (1), kurarinone (2), kurarinol (3), norkurarinol (4), and kuraridin (9) inhibited the generation of thiobarbituric acid reactive substances (TBARS) with IC50s of 7.9, 14.5, 22.0, 26.9, and 17.5 microM, respectively. The most potent inhibitor, compound 1, also demonstrated significant activities in complementary in vitro investigations, such as lag time (130 min at 5 microM), relative electrophoretic mobility (REM) of ox-LDL (80% inhibition at 20 microM), and fragmentation of apoB-100 (inhibition of 71% at 20 microM). Analysis of the structures of these compounds reveals that a resorcinol moiety in the B-ring is strongly correlated with protection of LDL-oxidation.
authors.affiliation: National Research Laboratory of Lipid Metabolism & Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Tae-Sook
authors.initials: TS
authors.lastname: Jeong
authors.forename: Young Bae
authors.initials: YB
authors.lastname: Ryu
authors.forename: Hoi Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Marcus John
authors.initials: MJ
authors.lastname: Curtis-Long
authors.forename: Sojin
authors.initials: S
authors.lastname: An
authors.forename: So Jin
authors.initials: SJ
authors.lastname: An
authors.forename: Jin Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Woo Song
authors.initials: WS
authors.lastname: Lee
authors.forename: Ki Hun
authors.initials: KH
authors.lastname: Park
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D053299:Apolipoprotein B-100 / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D019327:Copper Sulfate / Q000494:pharmacology; D024202:Electrophoretic Mobility Shift Assay; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D018517:Plant Roots / Q000737:chemistry; D029909:Sophora / Q000737:chemistry*; D013329:Structure-Activity Relationship; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D053299:Apolipoprotein B-100; D005419:Flavonoids; D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; D019327:Copper Sulfate