Fluorimetric determination of diacetyl and 2,3-pentanedione with isoniazide and a zirconium salt.
Diacetyl and 2,3-pentanedione react with isoniazide in acidified solutions to give the corresponding hydrazones which, by reacting with a zirconium (IV) salt, form fluorescent complexes. Both reactions have been studied separately. Variables such as pH, heating time, and excess of reactives are discussed. The method may be used for the determination of these compounds in foods.
García-Villanova
R J
RJ
Departamento de Nutrición y Bromatologia, Facultad de Farmacia, Universidad de Granada, Granada, Spain.
García Estepa
R M
RM