Methylated DNA immunoprecipitation (MeDIP).
Methylated DNA immunoprecipitation (MeDIP) is a versatile immunocapturing approach for unbiased detection of methylated DNA. In brief, genomic DNA is randomly sheared by sonication and immunoprecipitated with a monoclonal antibody that specifically recognizes 5-methylcytidine. The resulting enrichment of methylated DNA in the immunoprecipitated fraction can be determined by PCR to assess the methylation state of individual regions. Alternatively, MeDIP can be combined with large-scale analysis using microarrays as a genome-wide experimental readout. This protocol has been applied to generate comprehensive DNA methylation profiles on a genome-wide scale in mammals and plants, and further to identify abnormally methylated genes in cancer cells.
Mohn
Fabio
F
Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
Weber
Michael
M
Schübeler
Dirk
D
Roloff
Tim-Christoph
TC
D000818:Animals; D001483:Base Sequence; D018899:CpG Islands; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D014764:Viral Proteins
D017931:DNA Primers; C440255:Ocr protein, bacteriophage T7; D014764:Viral Proteins; D004247:DNA