Sequential injection spectrophotometric determination of trace amounts of iodide by its catalytic effect on the 4,4'-methylenebis(N,N-dimethylaniline)-chloramine-T reaction.
A simple and sensitive sequential injection spectrophotometric procedure is proposed for the determination of trace amounts of iodide in pharmaceutical preparations. The method is based on the catalytic effect of iodide on the (tetra base) 4,4'-methylenebis(N,N-dimethylaniline)-chloramine-T reaction in acidic solution. The method involves a sequential aspiration of 255mul sample/standard followed by 170mul tetra base and then 128mul chloramine-T solutions into a carrier stream to be stacked inside a holding coil and flow reversed through a reaction coil towards a detector. The resulting colored compound is measured at 600nm using an UV/Vis-spectrophotometer. All the parameters that affect the reaction were evaluated and the calibration curve is linear over a range of 0.1-6.0mugl(-1) of iodide concentration with detection limit of 0.05mugl(-1). A sample throughput of 80 samples per hour and relative standard deviation of less than 2.0% was achieved. The method is successfully applied for the determination of iodide in three different samples (tablets).
Tesfaldet
Zeriet O
ZO
Department of Chemistry, University of Pretoria, Pretoria 0002, South Africa.
van Staden
Jacobus F
JF
Stefan
Raluca I
RI