Antibody immobilization within glass microstructured fibers: a route to sensitive and selective biosensors.
Glass microstructured optical fibers have been rendered biologically active for the first time via the immobilization of antibodies within the holes in the fiber cross-section. This has been done by introducing coating layers to the internal surfaces of soft glass fibers. The detection of proteins that bind to these antibodies has been demonstrated experimentally within this system via the use of fluorescence labeling. The approach combines the sensitivity resulting from the long interaction lengths of filled fibers with the selectivity provided by the use of antibodies.
Ruan
Yinlan
Y
Centre of Expertise in Photonics, School of Chemistry & Physics, University of Adelaide, Adelaide, SA, Australia. yinlan.ruan@adelaide.edu.au
Foo
Tze Cheung
TC
Warren-Smith
Stephen
S
Hoffmann
Peter
P
Moore
Roger C
RC
Ebendorff-Heidepriem
Heike
H
Monro
Tanya M
TM
D000906:Antibodies / Q000378:metabolism*; D000936:Antigen-Antibody Complex; D015374:Biosensing Techniques; D005453:Fluorescence; D005898:Glass / Q000737:chemistry*; D012996:Solutions
D000906:Antibodies; D000936:Antigen-Antibody Complex; D012996:Solutions; C009753:fiberglass