Flow-injection liposome immunoanalysis (FILIA) for alachlor.
An automated Flow-Injection Liposome ImmunoAnalysis (FILIA) system has been modified from a previous design, using a specific environmental contaminant, the herbicide alachlor, as a model analyte. Signal amplification by means of fluorescent marker-loaded, analyte-tagged liposomes provides high sensitivity. The computer controlled system is composed of commercially available components, with the exception of the column packing material, which has to be prepared for each specific analyte to be determined. The use of such components means that the system is easily modified. The relationships between antibody concentration and assay speed and sensitivity are explored, and the possibilities of using the system for determination of multiple analytes is discussed.
Reeves
S G
SG
Analytical Chemistry Laboratories, Cornell University, Geneva, NY 14456-0462, USA.
Rule
G S
GS
Roberts
M A
MA
Edwards
A J
AJ
Durst
R A
RA