Investigating the biosynthetic origin of the nitro group in pyrrolomycins.
Feasible modes of introducing the nitro group into pyrrolomycin antibiotics were investigated based on incorporation of (15)N-labeled arginine and proline into dioxapyrrolomycin, produced by the actinomycete culture LL-F42248. Biosynthesis of nitrated pyrrolomycins was unaffected by the presence of nitric oxide synthase (NOS) inhibitors. The culture was able to grow in nitrogen-free (minimal) media and produce nitrated secondary metabolites. These results indicate that LL-F42248 is capable of fixing nitrogen.
Ratnayake
Anokha S
AS
Chemical and Screening Sciences, Wyeth Research, 401 North Middletown Road, Pearl River, New York 10965, USA. ratnaya@wyeth.com
Haltli
Bradley
B
Feng
Xidong
X
Bernan
Valerie S
VS
Singh
Maya P
MP
He
Haiyin
H
Carter
Guy T
GT
D000190:Actinomyces / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism / Q000494:pharmacology; D001272:Atmosphere / Q000737:chemistry; D015394:Molecular Structure; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D009584:Nitrogen / Q000378:metabolism; D009586:Nitrogen Fixation / Q000502:physiology*; D011758:Pyrroles / Q000737:chemistry / Q000494:pharmacology; D019323:omega-N-Methylarginine / Q000378:metabolism / Q000494:pharmacology
D011758:Pyrroles; D019323:omega-N-Methylarginine; C034690:pyrrolomycin C; D001120:Arginine; D019001:Nitric Oxide Synthase; D009584:Nitrogen