title: The effect of volume of chorionic villi on long-term cell culture.
abstract: OBJECTIVE
  It was the aim of our study to investigate the association between culture time and weight of villi obtained by transabdominal chorionic villus sampling (CVS).
  
  
  METHODS
  We analyzed 1,442 villus samples.
  
  
  RESULTS
  The gestational age at sampling ranged from 10 to 14 weeks. The weight of villi in these samples ranged from 1 to 80 mg (median 10 mg, interquatile range 7-12 mg). The culture time ranged from 5 to 24 days. Culture time was significantly and inversely correlated with the weight of villi obtained (r = -0.258, p < 0.01). Time was significantly longer when 5 mg or less of villi was obtained but the difference was only up to 2 days.
  
  
  CONCLUSIONS
  Because the difference in culture time for different groups of villus samples was within 1-2 days, this relationship is statistically significant but has no clinical significance.
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, SAR, China. drchenmin2003@yahoo.com.hk
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Lucy
authors.initials: L
authors.lastname: Ng
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Lau
authors.forename: Chin Peng
authors.initials: CP
authors.lastname: Lee
authors.forename: Mary Hoi Yin
authors.initials: MH
authors.lastname: Tang
authors.forename: Yung Hang
authors.initials: YH
authors.lastname: Lam
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002824:Chorionic Villi; D015193:Chorionic Villi Sampling / Q000379:methods*; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis* / Q000235:genetics; D005820:Genetic Testing; D005865:Gestational Age; D006801:Humans; D007621:Karyotyping; D009929:Organ Size; D011247:Pregnancy; D012189:Retrospective Studies; D013997:Time Factors