Electropolymerized m-phenylenediamine as a means to immobilize active protein on thickness-shear-mode quartz crystal.
Electropolymerized m-phenylenediamine was used as an active coating for immobilizing urease and lectin on a gold-plated thickness-shear-mode (TSM) crystal. To enhance effectiveness of immobilization. a bilayer polymer film composed of polyaniline and poly-m-phenylenediamine was proposed. Compared with single poly-m-phenylenediamine film, the bilayer polymer film gave better results in terms of immobilizing capacity, stability and reproductivity. On this bilayer-film-coated TSM quartz crystal, the amount of immobilized lectin was estimated about 1.8 mug/cm(2). Detection of purified human erythrocytes is demonstrated as an example of potential application of this lectin-modified TSM biosensor in clinic.
Si
S H
SH
New Material Research Institute, Hunan University, Changsha, 410082 P.R. China.
Xu
Y J
YJ
Nie
L H
LH
Yao
S Z
SZ