A label-free electrochemical immunosensor based on gold nanoparticles for detection of paraoxon.
An electrochemical immunosensor for the direct determination of paraoxon has been developed based on the biocomposites of gold nanoparticles loaded with paraoxon antibodies. The biocomposites are immobilized on the glassy carbon electrode (GCE) using Nafion membrane. On the immunosensor prepared paraoxon shows well-shaped CV with reduction and oxidation peaks located -0.08 and -0.03 mV versus SCE, respectively. The detection of paraoxon performed at -0.03 mV is beneficial for guaranteeing sufficient selectivity. The amount of the biocomposite consisting gold nanoparticles loaded with antibodies and the volume of Nafion solution used for fabricating the immunosensor have been studied to ensure sensitivity and conductivity of the immunosensor. The immunosensor has been employed for monitoring the concentrations of paraoxon in aqueous samples up to 1920 mug l(-1) with a detection limit of 12 mug l(-1).
Hu
Shun-Qin
SQ
State key laboratory for Chemo/Biosensing Technology and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China; Department of Chemistry, Zhu-Zhou Teachers' College, Zhuzhou 412007, China.
Xie
Jian-Wei
JW
Xu
Qin-Hui
QH
Rong
Kang-Tai
KT
Shen
Guo-Li
GL
Yu
Ru-Qin
RQ