TP53 gene mutations in canine osteosarcoma.
OBJECTIVE
To investigate mutations of the TP53 gene in canine osteosarcoma (OS).


METHODS
Clinical historic cohort study.


METHODS
Client-owned dogs.


METHODS
OS (n=59) were screened for mutations of the complete TP53 gene using polymerase chain reaction and the mutation was analyzed by single-strand conformational polymorphism. Clinical outcome of dogs with TP53-mutated OS were compared with dogs with OS without a mutation after complete surgical excision of the primary tumor.


RESULTS
TP53 gene mutations were observed in 24 of 59 (40.7%) OS; 3 mutated OS had 2 mutations. The alterations consisted mainly of point mutations (74%). Dogs with mutated OS had a significantly shorter survival time (ST) after surgery than dogs with normal tumor TP53 gene expression (P=.03). Other significant prognosticators for ST and disease-free interval included elevated serum alkaline phosphatase (P<.01) and tumor grade (P=.01).


CONCLUSIONS
TP53 genetic mutations are common in canine OS and may have a prognostic value.


CONCLUSIONS
Mutations of the TP53 gene may influence survival and should be considered when evaluating canine OS.
Kirpensteijn
Jolle
J
Department of Clinical Sciences of Companion Animals and Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. j.kirpensteijn@uu.nl
Kik
Marja
M
Teske
Erik
E
Rutteman
Gerard R
GR
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D014408:Biomarkers, Tumor; D001859:Bone Neoplasms / Q000235:genetics / Q000401:mortality / Q000601:surgery / Q000662:veterinary*; D018572:Disease-Free Survival; D004283:Dog Diseases / Q000235:genetics* / Q000401:mortality* / Q000601:surgery; D004285:Dogs; D005260:Female; D016158:Genes, p53 / Q000235:genetics; D053208:Kaplan-Meier Estimate; D008297:Male; D009154:Mutation; D009367:Neoplasm Staging / Q000662:veterinary; D012516:Osteosarcoma / Q000235:genetics / Q000401:mortality / Q000601:surgery / Q000662:veterinary*; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D018807:Polymorphism, Single-Stranded Conformational; D016896:Treatment Outcome
D014408:Biomarkers, Tumor; D000469:Alkaline Phosphatase