title: Arylesterase and paraoxonase activity of paraoxonase (PON1) affected by ischemia in the plasma of patients with arterial occlusion of the lower limbs.
abstract: OBJECTIVE
  The aim of the study was to evaluate the effect of different degrees of chronic ischemia of the lower limbs on PON1 activity in plasma, in relation to different substrates.
  
  
  METHODS
  The studied group consisted of patients with chronic arterial occlusion of the lower limbs due to atherosclerosis. The paraoxonase and arylesterase activities of PON1 were measured according to the Karen Gan method.
  
  
  RESULTS
  PON1 arylesterase activity was affected by ischemia of the lower limbs depending on its degree. In the group with critical ischemia decreased PON1 activity was observed in comparison with that in the moderate ischemia group and the control group (50.05+/-21.40 U/mL and 82.59+/-29.27 U/mL, 85.30+/-35.05 U/mL, respectively).
  
  
  CONCLUSIONS
  The results revealed a reverse relationship between PON1 activity and the progress of atherosclerosis to the ischemic level. The present study demonstrates, for the first time, that the arylesterase activity of PON1 is affected by critical ischemia of the lower limbs.
authors.affiliation: Department of General Chemistry, Chemistry and Clinical Biochemistry, Poznań University of Medical Sciences, 6 Grunwaldzka Street, 60-780 Poznań, Poland. magdarut@ump.edu.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kasprzak
authors.forename: Maria
authors.initials: M
authors.lastname: Iskra
authors.forename: Wacław
authors.initials: W
authors.lastname: Majewski
authors.forename: Tomasz
authors.initials: T
authors.lastname: Wielkoszyński
mesh_terms: D000368:Aged; D001157:Arterial Occlusive Diseases / Q000097:blood*; D043303:Aryldialkylphosphatase / Q000097:blood / Q000378:metabolism*; D050197:Atherosclerosis / Q000097:blood; D002265:Carboxylic Ester Hydrolases / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007511:Ischemia / Q000097:blood* / Q000503:physiopathology*; D035002:Lower Extremity / Q000098:blood supply*; D008297:Male; D008875:Middle Aged
chemical_list: D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human