title: [Screening of bioactive constituents from sea cucumber Holothuria nobilis using conidia of Pyricularia oryzae].
abstract: OBJECTIVE
  To study the bioactive triterpene glycosides from sea cucumber Holothuria nobilis.
  
  
  METHODS
  Guided by Pyricularia oryzae bioassay method, the glycosides compounds were separated by multi-chromatography, and their structures were elucidated by chemical and spectral analysis.
  
  
  RESULTS
  Twelve triterpene glycosides were obtained and their structures were identified, including seven novel saponins. All saponins showed activities on P. oryzae, and most of them exhibited cytotoxicity on P388 and A549 significantly.
  
  
  CONCLUSIONS
  Our researches provided valuable leads for pursuing new anticancer drugs, and established a homebase for further development of H. nobilis which is widely distributed in South China Sea.
authors.affiliation: Zhejiang Pharmaceutical College, Ningbo 315200, China. zjj@zjbti.net.cn
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Dai
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Chen
authors.forename: Ping-Yue
authors.initials: PY
authors.lastname: Ding
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D047332:Holothuria / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D007941:Leukemia P388 / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology; D008419:Materia Medica / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D003904:Mitosporic Fungi / Q000502:physiology*; D015394:Molecular Structure; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D008419:Materia Medica; D012503:Saponins