Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection.
A method of capillary electrophoresis with wall-jet amperometric detection (AD) has been developed for separation and determination of l-tyrosine (Tyr) and its metabolites, such as Tyramine (TA), p-hydroxyphenylpyruvic (pHPP), homogentisic acid (HGA) and some dipeptides containing Tyr, such as Tyr-Gly-Gly (YGG), Tyr-Arg (YR) and Tyr-d-Arg (Y-d-R). A carbon disk electrode was used as the working electrode and the optimal detection potential was 1.00V (versus Ag/AgCl). At 18kV of applied voltage, the seven compounds were completely separated within 20min in 110x10(-3)mol/L Na(2)HPO(4)-NaH(2)PO(4) buffer (pH 7.10) containing 3x10(-3)mol/L beta-cyclodextrin (beta-CD). Good linear relationship was obtained for all analytes and the detection limits of seven analytes were in the range of 0.95-4.25ng/mL. The proposed method has been applied to examine the metabolic process of l-tyrosine in rabbit's urine.
Huang
Ying
Y
The MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China; College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, Fujian 350007, China.
Jiang
Xiuyan
X
Wang
Wei
W
Duan
Jianping
J
Chen
Guonan
G