title: Evaluation of the influence of tissue parasite density on hematological and phenotypic cellular parameters of circulating leukocytes and splenocytes during ongoing canine visceral leishmaniasis.
abstract: During Leishmania infection, tissue parasitism at different sites may differ and imply distinct immunopathological patterns during canine visceral leishmaniasis (CVL). For this reason, we have assessed by flow cytometry the impact of spleen and skin parasite density on the phenotypic profile of splenocytes and circulating leukocytes of 40 Brazilian dogs naturally infected by Leishmania chagasi categorized according to splenic and cutaneous parasite load. Our major statistically significant findings demonstrated that dogs with splenic high parasitism presented a significant decrease in absolute counts of CD5+ T lymphocytes in comparison with dogs presenting splenic medium parasitism. Moreover, a decrease in the absolute number of circulating monocytes was observed as a hallmark of high parasitism. The increased frequency of CD8+ T cells is associated with low splenic parasitism during CVL. Although we did not found any significant differences between the immunophenotypic analysis performed in circulating lymphocytes according to cutaneous parasite load, there were negative correlations between CD5+ and CD8+ T cells and cutaneous parasite density reemphasizes the role of T cell-mediated immune response in resistance mechanisms during ongoing CVL. These results add new insights about the pathogenesis of CVL and may help in the establishment of additional tools for future studies on drugs and vaccine approaches.
authors.affiliation: Laboratório de Imunologia Celular e Molecular, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: L L
authors.initials: LL
authors.lastname: Guerra
authors.forename: A
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.forename: R C
authors.initials: RC
authors.lastname: Giunchetti
authors.forename: O A
authors.initials: OA
authors.lastname: Martins-Filho
authors.forename: A B
authors.initials: AB
authors.lastname: Reis
authors.forename: R
authors.initials: R
authors.lastname: Corrêa-Oliveira
mesh_terms: D000818:Animals; D018956:CD5 Antigens / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004283:Dog Diseases / Q000276:immunology* / Q000469:parasitology*; D004285:Dogs; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D012867:Skin / Q000469:parasitology; D013154:Spleen / Q000469:parasitology
chemical_list: D018956:CD5 Antigens