title: Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of microbial community structure in landfill leachate.
abstract: The structures of microbial communities in water samples obtained from a landfill site that had been a source of environmental pollution by emitting hydrogen sulfide were elucidated using polymerase chain reaction-denaturing gradient gel electrophoresis. The microbial communities, which consisted of a limited number of major microorganisms, were stable for several months. Microorganisms capable of degrading such chemical compounds as 2-hydroxybenzothiazole and bisphenol A were observed in landfill leachate. Microorganisms responsible for the production of hydrogen sulfide were not the primary microbes detected, even in water samples obtained from the site of gas emission.
authors.affiliation: Department of Environmental Chemistry and Ecoengineering, Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan. miho@tohtech.ac.jp
authors.forename: Miho
authors.initials: M
authors.lastname: Uchida
authors.forename: Haruna
authors.initials: H
authors.lastname: Hatayoshi
authors.forename: Aoi
authors.initials: A
authors.lastname: Syuku-nobe
authors.forename: Takefumi
authors.initials: T
authors.lastname: Shimoyama
authors.forename: Toru
authors.initials: T
authors.lastname: Nakayama
authors.forename: Akitsugu
authors.initials: A
authors.lastname: Okuwaki
authors.forename: Tokuzo
authors.initials: T
authors.lastname: Nishino
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hemmi
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001431:Bacteriological Techniques / Q000379:methods*; D001559:Benzhydryl Compounds; D052160:Benzothiazoles / Q000378:metabolism; D004586:Electrophoresis; D004787:Environmental Pollution; D006862:Hydrogen Sulfide; D010636:Phenols / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052160:Benzothiazoles; D006862:Hydrogen Sulfide; D010636:Phenols; D014874:Water Pollutants, Chemical; C005465:benzothiazole; C006780:bisphenol A; D000098826:Bisphenol A Compounds