Polarography of B(12) coenzyme.
The polarography of B(12) coenzyme (5,6-dimethylbenzimidazolylcobamide, DBC coenzyme) has been investigated at the dropping mercury electrode. Exposure of solutions of the coenzyme to light and then to oxygen give polarograms comparable to those of B(12r) and hydroxocobalamin (B(12a)) respectively. At pH 11.6 the coenzyme gives two reduction waves, at -1.43 and -1.62 V vs. the S.C.E.; in less basic solutions the two waves merge to give one multielectron wave.
Kratochvil
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B
Department of Chemistry, Iowa State University, Ames, Iowa, U.S.A.
Diehl
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