Photochemical analysis studies-V Photochemical enhancement of the fluorescence signal of the antimalarial plasmocid in liquid solution and on silica-gel thin layers.
Ultraviolet irradiation converts the antimalarial plasmocid into a highly fluorescent photoproduct. The effect of pH, solvent composition and irradiation time on the photochemical enhancement of the fluorescence signal of plasmocid has been investigated in solution and on silica-gell thin layers. A significant increase in the fluorescence intensity in very acidic medium is explained by the formation of a triprotonated plasmocid species. The photochemical-fluorescence enhancement factors are particularly large, ranging between about 10 and 20 on silica-gel thin layers. Linear calibration plots are obtained over a concentration range of nearly three orders of magnitude. The photochemical-fluorimetric detection limit of plasmocid on thin layers is 10 ng. The results presented demonstrate the potential of thin-layer phototochemical fluorimetry as a sensitive analytical method.
Aaron
J J
JJ
Laboratoire de Chimie Physique Organique et d'Analyse Instrumentale, Département de Chimie, Faculté des Sciences, Université de Dakar, Dakar-Fann, SénégalFrance.
Pidanza
J
J
Gaye
M D
MD