title: Quantification of sex pheromone from Anocentor nitens by gas chromatography-mass spectrometry-selected ion monitoring.
abstract: Two highly sensitive and selective methods based on gas chromatography coupled to mass spectrometry (GC-MS) in the selected ion monitoring (SIM) mode have been developed for the quantification of 2,6-dichlorophenol (2,6-DCP), a sex pheromone of the tick females of Anocentor nitens. Standard addition method and calibration curve techniques using 5-bromine-4-hydroxy-3-methoxybenzaldehyde (5-BrV) as internal standard (IS) afforded detection limit of 0.1ngml(-1). The calibration curve was linear over the concentration range from 0.5 to 500ngml(-1) for 2,6-DCP. Results show that the concentration range of sex pheromone in the extracts samples was 1.08-10.35ngml(-1). The methods developed provided reliable procedures to determine amounts of 2,6-DCP present in ticks.
authors.affiliation: Instituto de Quimica, Universidade Federal de Goiás, C.P. 131, 74001-970 Goiânia, GO, Brazil.
authors.forename: Clenilson M
authors.initials: CM
authors.lastname: Rodrigues
authors.forename: Lígia M F
authors.initials: LM
authors.lastname: Borges
authors.forename: Pedro H
authors.initials: PH
authors.lastname: Ferri
authors.forename: Lourival C
authors.initials: LC
authors.lastname: Faria