title: A recombinant replication-defective human adenovirus type 3: a vaccine candidate.
abstract: Human adenovirus type 3 (HAdV-3) cause respiratory infections globally. There is no safe and efficient HAdV-3 vaccine thus far. In this report, a replication-defective human adenovirus type 3 was constructed by deletion of the whole E1 gene. A HEp-2 cell-based cell line designated HEp-2/E1 was generated and was shown to complement the function of E1 deleted mutant. A partial genome (9.5-100 mu) of HAdV-3 was cloned into pPolyII vector to be a backbone plasmid. A shuttle vector carrying eGFP gene was also constructed. The backbone and shuttle plasmids were co-transfected into the HEp-2/E1 cell line and 293 cell line separately. Typical cytopathic effect (CPE) appeared in the HEp-2/E1, but not in 293 cells 2 weeks after transfection. Wild-type HAdV-3 is neutralized by the sera from the mice immunized with the recombinant HAdV-3. The results demonstrated that the recombinant adenovirus type 3 may serve as a HAdV-3 vaccine candidate.
authors.affiliation: Central Laboratory, Guangzhou Children's Hospital, 318 Renmin Zhong Road, Guangzhou 510120, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.forename: Jingxian
authors.initials: J
authors.lastname: Chen
authors.forename: Xingui
authors.initials: X
authors.lastname: Tian
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Zhang
authors.forename: Qiyi
authors.initials: Q
authors.lastname: Zeng
authors.forename: Sitang
authors.initials: S
authors.lastname: Gong
mesh_terms: D000260:Adenoviruses, Human / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D003673:Defective Viruses / Q000235:genetics* / Q000276:immunology*; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D014765:Viral Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D014779:Virus Replication / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014765:Viral Vaccines; D049452:Green Fluorescent Proteins