title: Microarray analysis of gene expression profile induced by the biocontrol yeast Cryptococcus laurentii in cherry tomato fruit.
abstract: To obtain an overall view on transcript modification during the cherry tomato fruit responding to biocontrol yeast Cryptococcus laurentii, we performed a microarrary analysis, using Affymetrix Tomato Genechip arrays, representing approximately 10,000 genes. The results showed that 194 and 312 genes were up- or down-regulated, respectively, more then ten time fold (log2 change ratio>or=2 or log2 change ratio<or=-2) in biocontrol yeast treated tomato fruit as compared with control fruits. Those up-regulated included genes involved in metabolism, signal transduction, and stress response. Conversely, genes related to energy metabolism and photosynthesis were generally down-regulated. Our results suggest that biocontrol yeast treatment induces fruit resistance response, suppresses energy metabolism and photosynthesis. A number of transcripts encoding proteins/enzymes which are known to be up-regulated under some biotic and abiotic stress are also up-regulated after the applying biological control yeast to cherry tomato fruit. The expression of these proteins might increase the fruit resistance towards postharvest pathogen infection and damage.
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou, 310029, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zheng
authors.forename: Jishuang
authors.initials: J
authors.lastname: Chen
mesh_terms: D003454:Cryptococcus / Q000502:physiology*; D005638:Fruit / Q000235:genetics* / Q000382:microbiology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis; D010572:Pest Control, Biological; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger