title: Rapid access to genes of biotechnologically useful enzymes by partial genome sequencing: the thermoalkaliphile Anaerobranca gottschalkii.
abstract: Anaerobranca gottschalkii strain LBS3 T is an extremophile living at high temperature (up to 65 degrees C) and in alkaline environments (up to pH 10.5). An assembly of 696 DNA contigs representing about 96% of the 2.26-Mbp genome of A. gottschalkii has been generated with a low-sequence-coverage shotgun-sequencing strategy. The chosen sequencing strategy provided rapid and economical access to genes encoding key enzymes of the mono- and polysaccharide metabolism, without dilution of spare resources for extensive sequencing of genes lacking potential economical value. Five of these amylolytic enzymes of considerable commercial interest for biotechnological applications have been expressed and characterized in more detail after identification of their genes in the partial genome sequence: type I pullulanase, cyclodextrin glycosyltransferase (CGTase), two alpha-amylases (AmyA and AmyB), and an alpha-1,4-glucan-branching enzyme.
authors.affiliation: Institute of Technical Microbiology, Hamburg University of Technology, Hamburg, Germany. antranikian@tuhh.de or hpk@dsmz.de
authors.forename: G
authors.initials: G
authors.lastname: Antranikian
authors.forename: A
authors.initials: A
authors.lastname: Ruepp
authors.forename: P M K
authors.initials: PM
authors.lastname: Gordon
authors.forename: M
authors.initials: M
authors.lastname: Ballschmiter
authors.forename: A
authors.initials: A
authors.lastname: Zibat
authors.forename: M
authors.initials: M
authors.lastname: Stark
authors.forename: C W
authors.initials: CW
authors.lastname: Sensen
authors.forename: D
authors.initials: D
authors.lastname: Frishman
authors.forename: W
authors.initials: W
authors.lastname: Liebl
authors.forename: H-P
authors.initials: HP
authors.lastname: Klenk
mesh_terms: D001709:Biotechnology; D004798:Enzymes / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D006094:Gram-Positive Bacteria / Q000201:enzymology* / Q000235:genetics*; D000516:alpha-Amylases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D004798:Enzymes; D000516:alpha-Amylases