Characteristics of persistent Salmonella Enteritidis strains in two integrated broiler chicken operations of Korea.
The objectives of the study were to investigate the phenotypic and genotypic characterization of the persistent Salmonella Enteritidis (S. Enteritidis) isolates in two integrated broiler chicken operations, with attention focused mainly on the epidemiological approach. In the distribution of virulence genes, Salmonella enterotoxin (stn), invading host cell (invA), and Salmonella plasmid virulence (spvC) genes were widely distributed among the S. Enteritidis irrespective of their source of isolation, and Salmonella fimbrial (sefC) and plasmid encoded fimbrial (pef) genes were present in 28 and 20 S. Enteritidis strains, respectively. A total of 5 different XbaI-PFGE types were obtained from 31 S. Enteritidis isolates. Twenty-one types were divided on the basis their PFGE pattern, phage type and antimicrobial resistance pattern determined. There was a significant difference in phenotypic and genotypic characterization by two integrated broiler operations. Also, 8 isolates shown susceptible to all antimicrobials and 11 isolates with resistance to nalidixic acid were partly classified by XbaI PFGE pattern and by the phage type.
Kwag
Sang Ick
SI
Animal Health Division, Ministry of Agriculture & Forestry, Gwacheon, Republic of Korea.
Bae
Dong Hwa
DH
Cho
Jae Keun
JK
Lee
Hee Soo
HS
Ku
Bok Gyeong
BG
Kim
Byoung Han
BH
Cho
Gil-Jae
GJ
Lee
Young Ju
YJ
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001434:Bacteriophage Typing / Q000662:veterinary; D002645:Chickens; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D004768:Enterotoxins / Q000235:genetics; D036781:Fimbriae Proteins / Q000235:genetics; D007723:Korea / Q000453:epidemiology; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D015995:Prevalence; D012481:Salmonella Infections, Animal / Q000453:epidemiology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004768:Enterotoxins; C082864:enterotoxin, Salmonella; C080933:sefC protein, Salmonella enteritidis; C075518:invA protein, Bacteria; D036781:Fimbriae Proteins