title: Expression and transport of Angiotensin II AT1 receptors in spinal cord, dorsal root ganglia and sciatic nerve of the rat.
abstract: To clarify the role of Angiotensin II in the regulation of peripheral sensory and motor systems, we initiated a study of the expression, localization and transport of Angiotensin II receptor types in the rat sciatic nerve pathway, including L(4)-L(5) spinal cord segments, the corresponding dorsal root ganglia (DRGs) and the sciatic nerve. We used quantitative autoradiography for AT(1) and AT(2) receptors, and in situ hybridization to detect AT(1A), AT(1B) and AT(2) mRNAs. We found substantial expression and discrete localization of Angiotensin II AT(1) receptors, with much higher numbers in the grey than in the white matter. A very high AT(1) receptor expression was detected in the superficial dorsal horns and in neuronal clusters of the DRGs. Expression of AT(1A) mRNA was significantly higher than that of AT(1B). AT(1) receptor binding and AT(1A) and AT(1B) mRNAs were especially prominent in ventral horn motor neurons, and in the DRG neuronal cells. Unilateral dorsal rhizotomy significantly reduced AT(1) receptor binding in the ipsilateral side of the superficial dorsal horn, indicating that a substantial number of dorsal horn AT(1) receptors have their origin in the DRGs. After ligation of the sciatic nerve, there was a high accumulation of AT(1) receptors proximal to the ligature, a demonstration of anterograde receptor transport. We found inconsistent levels of AT(2) receptor binding and mRNA. Our results suggest multiple roles of Angiotensin II AT(1) receptors in the regulation of sensory and motor functions.
authors.affiliation: Section on Pharmacology, National Institute of Mental Health, Bethesda, MD 20892, USA. pavelj@mail.nih.gov
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Pavel
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.forename: Stephen
authors.initials: S
authors.lastname: Brimijoin
authors.forename: Armen
authors.initials: A
authors.lastname: Moughamian
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nishioku
authors.forename: Julius
authors.initials: J
authors.lastname: Benicky
authors.forename: Juan M
authors.initials: JM
authors.lastname: Saavedra
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000870:Anterior Horn Cells / Q000378:metabolism; D001345:Autoradiography; D005727:Ganglia, Spinal / Q000378:metabolism*; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D009434:Neural Pathways / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics* / Q000378:metabolism*; D044139:Receptor, Angiotensin, Type 2 / Q000235:genetics / Q000378:metabolism; D019051:Rhizotomy; D012584:Sciatic Nerve / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2