Immunoassay by detecting enhanced resonance light scattering signals of immunocomplex using a common spectrofluorometer.
A sensitive, highly specific immunoassay method has been developed by measuring the enhanced resonance light scattering (RLS) signals of immunoreactions with simultaneously scanning both the excitation and the emission monochromators of a common spectrofluorometer. For a given content of antibody (Ab), the RLS signals of an immunoreaction follow Gaussian distribution with antigen (Ag) concentration. The central position of the Gaussian curve represents the concentration of given Ab, and the half bandwidth has proved to be a characteristic constant of a given Ab-Ag immunoreaction. With the RLS signals, the limit of detection for human immunoglobulin G (HIgG) in serum samples could reach 10ngml(-1), and the concentration of HIgG in blood serum samples could be detected with the recovery of 90.2-107.7% and R.S.D. of 0.8-2.7%. The results of determination for three human serum samples are identical to those obtained by immunoturbidimetry.
Zhao
Hua Wen
HW
Laboratory of Molecular Chemistry and Chemical Biology, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China; Department of Chemistry, Third Military Medical University, Chongqing 400038, China.
Huang
Cheng Zhi
CZ
Li
Yuan Fang
YF