A simple and rapid fluorimetric method for the microdetermination of isonicotinic acid hydrazide.
A simple, rapid and sensitive fluorimetric method has been developed for the microdetermination of isonicotinic acid hydrazide, based on its formation of a hydrazone with 2-hydroxy-1-naphthaldehyde in acidic medium, in the presence of excess of scandium ions, and the consequent formation of a strongly fluorescent complex (lambda(ex) 430 nm, lambda(em) 510 nm) between the hydrazone and scandium in weakly acidic medium. Kinetic and equilibrium procedures for isonicotinic acid hydrazide determination are proposed. A calibration graph linear up to 5.00 mug/ml is obtained by both procedures, with a mean relative error of about 2.0%. The detection limit for the kinetic procedure is 0.4 ng/ml and for the equilibrium procedure about 0.2 ng/ml.
Ioannou
P C
PC
Laboratory of Analytical Chemistry, Chemistry Department, University of Athens, 104 Solonos St. Athens 10680, Greece.