[Qufeng Tongluo decoction inhibits lipopolysaccharide-induced rat mesangial cell proliferation in vitro by suppressing NF-kappaB activation and reducing TGF-beta1 and IL-6 mRNA expressions].
OBJECTIVE
To investigate the effect of Qufeng Tongluo (QFTL) decoction on lipopolysaccharide (LPS)-induced rat mesangial cell proliferation and explore the possible mechanisms underlying the therapeutic effects.


METHODS
Thirty-two rats were randomized into normal control, glomerulonephritis model, QFTL treatment and positive control groups, and serum samples were obtained from these groups. Rat mesangial cells with or without LPS exposure were treated with the sera, and the expression of nuclear factor-kappaB (NF-kappaB ) was detected using electrophoretic mobility shift assay (EMSA), and the expressions of transforming growth factor-beta1 (TGF-beta1) and interleukin-6 (IL-6) mRNAs were detected with RT-PCR.


RESULTS
QFTL decoction inhibited the activation of NF-kappaB in LPS-stimulated rat mesangial cells stimulated by LSP, and lowered the expressions of TGF-beta1 and IL-6 mRNA.


CONCLUSIONS
QFTL decoction can inhibit LPS-induced rat mesangial cell proliferation by decreasing the expression of TGF-beta1 and IL-6 mRNA as a result of suppression NF-kappaB activation.
Wang
Zhu
Z
Department of Integrated Traditional Chinese and Western Medicine, Second Hospital Affiliated to Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. netsyy 2003@yahoo.com.cn
Sun
Wan-sen
WS
Wang
Juan
J
Ma
Qiao-ya
QY
Zhang
Xiao-qiang
XQ
Wu
Xi-li
XL
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005921:Glomerulonephritis / Q000473:pathology*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D050527:Mesangial Cells / Q000187:drug effects* / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; C527174:tongluo