title: Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts.
abstract: BACKGROUND
  This study evaluated changes in platelet-derived growth factor (PDGF)-AB and transforming growth factor (TGF)-beta1 release from platelets by platelet-rich plasma activation, and the proliferation potential of activated platelet-rich plasma and platelet-poor plasma on human adipose-derived stem cells and human dermal fibroblasts.
  
  
  METHODS
  Platelet-rich plasma was prepared using a double-spin method, with the number of platelets counted in each preparation stage. Platelet-rich and platelet-poor plasma were activated with autologous thrombin and calcium chloride, and levels of platelet-released PDGF-AB and TGF-beta1 were determined by enzyme-linked immunosorbent assay. Cells were cultured for 1, 4, or 7 days in serum-free Dulbecco's Modified Eagle Medium supplemented with 5% whole blood plasma, nonactivated platelet-rich plasma, nonactivated platelet-poor plasma, activated platelet-rich plasma, or activated platelet-poor plasma. In parallel, these cells were cultured for 1, 4, or 7 days in serum-free Dulbecco's Modified Eagle Medium supplemented with 1%, 5%, 10%, or 20% activated platelet-rich plasma. The cultured human adipose-derived stem cells and human dermal fibroblasts were assayed for proliferation.
  
  
  RESULTS
  Platelet-rich plasma contained approximately 7.9 times as many platelets as whole blood, and its activation was associated with the release of large amounts of PDGF-AB and TGF-beta1. Adding activated platelet-rich or platelet-poor plasma significantly promoted the proliferation of human adipose-derived stem cells and human dermal fibroblasts. Adding 5% activated platelet-rich plasma to the medium maximally promoted cell proliferation, but activated platelet-rich plasma at 20% did not promote it.
  
  
  CONCLUSIONS
  Platelet-rich plasma can enhance the proliferation of human adipose-derived stem cells and human dermal fibroblasts. These results support clinical platelet-rich plasma application for cell-based, soft-tissue engineering and wound healing.
authors.affiliation: Osaka, Japan From the Department of Plastic and Reconstructive Surgery, Kansai Medical University.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Kakudo
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Minakata
authors.forename: Toshihito
authors.initials: T
authors.lastname: Mitsui
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kushida
authors.forename: Frederik Zefanya
authors.initials: FZ
authors.lastname: Notodihardjo
authors.forename: Kenji
authors.initials: K
authors.lastname: Kusumoto
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D053687:Adult Stem Cells / Q000166:cytology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D015539:Platelet Activation; D010976:Platelet Count; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D053657:Platelet-Rich Plasma; D050151:Subcutaneous Fat / Q000166:cytology; D013917:Thrombin / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; C095477:platelet-derived growth factor AB; D013917:Thrombin; D002122:Calcium Chloride