title: Salinimicrobium terrae sp. nov., isolated from saline soil, and emended description of the genus Salinimicrobium.
abstract: A novel yellow-pigmented, Gram-negative, slightly halophilic, catalase-positive, oxidase-negative, obligately aerobic bacterium, designated strain YIM-C338(T), was isolated from saline soil from the Qaidam Basin in north-west China. Cells were non-sporulating, non-motile, short rods, predominantly occurring singly. Coccoid bodies and slightly curved rod-shaped cells of varying length developed in older cultures. Growth occurred with 0.5-8 % (w/v) NaCl (optimally with 1-3 %, w/v), at pH 6.0-9.0 (optimally at pH 7.0) and at 4-37 degrees C (optimally at 28 degrees C). The major cellular fatty acids were C(16 : 0), C(18 : 0), C(16 : 1)omega7c, C(18 : 1)omega7c, iso-C(13 : 0) 3-OH and iso-C(15 : 1). MK-6 was the only respiratory quinone. Non-diffusible carotenoid pigments were produced. Flexirubin-type pigments were absent. The genomic DNA G+C content was 42.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM-C338(T) was a member of the genus Salinimicrobium, having sequence similarities of 97.3 and 94.0 % with respect to Salinimicrobium xinjiangense BH206(T) and Salinimicrobium catena HY1(T). The combination of the phylogenetic data, phenotypic characteristics and chemotaxonomic differences supported the view that strain YIM-C338(T) represents a novel species of the genus Salinimicrobium, for which the name Salinimicrobium terrae sp. nov. is proposed. The type strain is YIM-C338(T) (=DSM 17865(T) =CGMCC 1.6308(T)). An emended description of the genus Salinimicrobium is also provided.
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Yi-Guang
authors.initials: YG
authors.lastname: Chen
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Cui
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Yong-Xia
authors.initials: YX
authors.lastname: Wang
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.forename: Jee-Min
authors.initials: JM
authors.lastname: Lim
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Jiang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride