Enhanced chemiluminescence determination of alpha-amylase by use of amylose labelled with horseradish peroxidase.
An enhanced chemiluminescence method for determining soluble and immobilized alpha-amylase has been developed, based on use of an insoluble amylose substrate labelled with horseradish peroxidase. Soluble peroxidase-labelled fragments of the substrate, released by the action of alpha-amylase, are quantified by the peroxidase-catalyzed luminol-peroxide-p-iodophenol reaction. The detection limit for alpha-amylase was 125 fmole (12.5 nM). An insoluble amylopectin labelled with horseradish peroxidase was also effective as a substrate for this type of assay.
Kricka
L J
LJ
Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, PA 19104, U.S.A.
Marcinkowski
J M
JM
Wilding
P
P
Lekhakula
S
S