title: [Relationship between the expression of TrkB and anoikis resistance in nasopharyngeal carcinoma line].
abstract: OBJECTIVE
  To study the regulation of anoikis by tyrosine kinase receptor B (TrkB) in human nasopharyngeal carcinoma lines. METHODS; Expression levels of TrkB and brain-derived neurotrophic factor (BDNF) were evaluated by RT-PCR and Western blot. Colony formation ability of C666-1 was observed in soft agar. Proliferation rate and apoptosis, that change in cells by treating the TrkB inhibitor K252a and specificity ligand BDNF respectively under suspension culture, were measured by cell counting kit-8 (CCK-8) assay and the flow cytometry assay. The expression change of TrkB, BDNF and phosphorylation of serine threonine kinase (p-Akt) were investigated by Western blot analysis.
  
  
  RESULTS
  TrkB and BDNF were identified in C666-1 cells. C666-1 cells could be decreased the proliferation of colony in soft agar by effect of K252a, but BDNF could make the colony prolific. K252a can inhibit the expression of TrkB in C666-1, and prevent p-Akt activation. And exogenous BDNF stimulated up-regulation TrkB and p-Akt, induced anoikis resistance.
  
  
  CONCLUSIONS
  TrkB inhibits anoikis in nasopharyngeal carcinoma cells. Inhibiton of TrkB by K252a can induce anoikis, and may prove particularly effective in treatment of nasopharyngeal carcinoma.
authors.affiliation: Department of Otorhinolaryngology and Head Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Wen
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Xie
authors.forename: Xiang
authors.initials: X
authors.lastname: Yi
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhao
authors.forename: Zhi-Wen
authors.initials: ZW
authors.lastname: Xu
authors.forename: An-Zhou
authors.initials: AZ
authors.lastname: Tang
authors.forename: Guang-Wu
authors.initials: GW
authors.lastname: Huang
mesh_terms: D023102:Anoikis; D002277:Carcinoma; D045744:Cell Line, Tumor; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology; D020813:Receptor, trkB / Q000378:metabolism*
chemical_list: D020813:Receptor, trkB