title: TC1 (C8orf4) is involved in ERK1/2 pathway-regulated G(1)- to S-phase transition.
abstract: Although previous studies have implicated a role for TC1 (C8orf4) in cancer cell proliferation, the molecular mechanism of its action is still largely unclear. In this study, we showed, for the first time, that the mRNA levels of TC1 were upregulated by mitogens (FBS/thrombin) and at least partially, through the ERK1/2 signaling pathway. Interestingly, the over-expression of TC1 promoted the G(1)- to S-phase transition of the cell cycle, which was delayed by the deficiency of ERK1/2 signaling in fibroblast cells. Furthermore, the luciferase reporter assay indicated that the over-expression of TC1 significantly increased Cyclin D1 promoter-driven luciferase activity. Taken together, our findings revealed that TC1 was involved in the mitogen-activated ERK1/2 signaling pathway and positively regulated G(1)- to S-phase transition of the cell cycle. Our results may provide a novel mechanism of the role of TC1 in the regulation of cell proliferation.
authors.affiliation: Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Yi-Dong
authors.initials: YD
authors.lastname: Wang
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Bian
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Lv
authors.forename: Rong
authors.initials: R
authors.lastname: Zheng
authors.forename: Huan
authors.initials: H
authors.lastname: Sun
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.forename: Qin-Wei
authors.initials: QW
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.forename: Qiu-Tan
authors.initials: QT
authors.lastname: Yang
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Ai
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Wei
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000818:Animals; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D016193:G1 Phase / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D008934:Mitogens / Q000494:pharmacology; D041681:NIH 3T3 Cells; D009363:Neoplasm Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D016196:S Phase / Q000187:drug effects; D013917:Thrombin / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D005419:Flavonoids; D008934:Mitogens; D009363:Neoplasm Proteins; C418741:TCIM protein, human; C534905:Tcim protein, mouse; D019938:Cyclin D1; D008156:Luciferases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D013917:Thrombin; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one