title: High-performance liquid chromatographic assay of fleroxacin in human serum using fluorescence detection.
abstract: A HPLC method has been developed for the direct assay of fleroxacin in serum, without previous extraction. Serum samples, after the addition of sodium dodecylsulfate (0.5%), were injected directly into an LC Hisep column. The mobile phase consisted of acetonitrile, water and triethylamine in a per cent volume ratio 18:80:2. The pH of the mobile phase was adjusted to 6.50 with the addition of phosphoric acid. The drug was detected fluorometrically at lambda (ex )=280 nm and lambda (em )=450 nm . The linear concentration range of fleroxacin was between 0.01 and 2.0mg/l with a detection limit of 1ng/ml.
authors.affiliation: Faculty of Science, P.O. Box 60, 34000 Kragujevac, Yugoslavia.
authors.forename: P
authors.initials: P
authors.lastname: Djurdjevic
authors.forename: M
authors.initials: M
authors.lastname: Jelikic-Stankov
authors.forename: A
authors.initials: A
authors.lastname: Laban