title: Genome-wide association analysis reveals putative Alzheimer's disease susceptibility loci in addition to APOE.
abstract: Alzheimer's disease (AD) is a genetically complex and heterogeneous disorder. To date four genes have been established to either cause early-onset autosomal-dominant AD (APP, PSEN1, and PSEN2(1-4)) or to increase susceptibility for late-onset AD (APOE5). However, the heritability of late-onset AD is as high as 80%, (6) and much of the phenotypic variance remains unexplained to date. We performed a genome-wide association (GWA) analysis using 484,522 single-nucleotide polymorphisms (SNPs) on a large (1,376 samples from 410 families) sample of AD families of self-reported European descent. We identified five SNPs showing either significant or marginally significant genome-wide association with a multivariate phenotype combining affection status and onset age. One of these signals (p = 5.7 x 10(-14)) was elicited by SNP rs4420638 and probably reflects APOE-epsilon4, which maps 11 kb proximal (r2 = 0.78). The other four signals were tested in three additional independent AD family samples composed of nearly 2700 individuals from almost 900 families. Two of these SNPs showed significant association in the replication samples (combined p values 0.007 and 0.00002). The SNP (rs11159647, on chromosome 14q31) with the strongest association signal also showed evidence of association with the same allele in GWA data generated in an independent sample of approximately 1,400 AD cases and controls (p = 0.04). Although the precise identity of the underlying locus(i) remains elusive, our study provides compelling evidence for the existence of at least one previously undescribed AD gene that, like APOE-epsilon4, primarily acts as a modifier of onset age.
authors.affiliation: Genetics and Aging Research Unit, Mass General Institute for Neurodegenerative Disease (MIND), Department of Neurology, Massachusetts General Hospital, Charlestown, MA 02129, USA.
authors.forename: Lars
authors.initials: L
authors.lastname: Bertram
authors.forename: Christoph
authors.initials: C
authors.lastname: Lange
authors.forename: Kristina
authors.initials: K
authors.lastname: Mullin
authors.forename: Michele
authors.initials: M
authors.lastname: Parkinson
authors.forename: Monica
authors.initials: M
authors.lastname: Hsiao
authors.forename: Meghan F
authors.initials: MF
authors.lastname: Hogan
authors.forename: Brit M M
authors.initials: BM
authors.lastname: Schjeide
authors.forename: Basavaraj
authors.initials: B
authors.lastname: Hooli
authors.forename: Jason
authors.initials: J
authors.lastname: Divito
authors.forename: Iuliana
authors.initials: I
authors.lastname: Ionita
authors.forename: Hongyu
authors.initials: H
authors.lastname: Jiang
authors.forename: Nan
authors.initials: N
authors.lastname: Laird
authors.forename: Thomas
authors.initials: T
authors.lastname: Moscarillo
authors.forename: Kari L
authors.initials: KL
authors.lastname: Ohlsen
authors.forename: Kathryn
authors.initials: K
authors.lastname: Elliott
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Diane
authors.initials: D
authors.lastname: Hu-Lince
authors.forename: Marie
authors.initials: M
authors.lastname: Ryder
authors.forename: Amy
authors.initials: A
authors.lastname: Murphy
authors.forename: Steven L
authors.initials: SL
authors.lastname: Wagner
authors.forename: Deborah
authors.initials: D
authors.lastname: Blacker
authors.forename: K David
authors.initials: KD
authors.lastname: Becker
authors.forename: Rudolph E
authors.initials: RE
authors.lastname: Tanzi
mesh_terms: D017668:Age of Onset; D000465:Algorithms; D000483:Alleles; D000544:Alzheimer Disease / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics*; D001499:Bayes Theorem; D016022:Case-Control Studies; D002883:Chromosomes, Human, Pair 14; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D006801:Humans; D016014:Linear Models; D015810:Linkage Disequilibrium; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide; D063268:Sialic Acid Binding Ig-like Lectin 3 / Q000235:genetics; D044465:White People
chemical_list: D001057:Apolipoproteins E; C571163:CD33 protein, human; D005819:Genetic Markers; D063268:Sialic Acid Binding Ig-like Lectin 3