A rapid method for the estimation of glutamic acid with chloramine-T.
Chloramine-T (added in excess) oxidizes glutamic acid in various solvent media and the reaction is rapid and stoichiometric with a 4-electron change in buffers of pH 1-6, in 0.01 M sulphuric and perchloric acids and in 0.1M hydrochloric acid. A back-titration procedure using a pH-4 buffer or 0.1M hydrochloric acid as reaction medium has been developed. rho-Toluenesulphonamide and a nitrile have been identified in the reaction products. The effect of other species on the oxidation has been investigated.
Katgeri
S N
SN
Department of Post-graduate Studies and Research in Chemistry, Manasa Gangothri, Mysore-570006, India.
Mahadevappa
D S
DS
Naidu
H M
HM