A new study of the enzymatic hydrolysis of carboxymethyl cellulose with a bulk acoustic wave sensor.
A new bulk acoustic wave (BAW) cellulase sensing technique, which is based on the enzymatic hydrolysis process of sodium carboxymethylcellulose (CMC) by cellulase, was established. The frequency shift curves of BAW sensor indicated that the viscosity of the tested solutions decreased during the hydrolysis process. The hydrolysis rate of CMC by cellulase was calculated from the frequency shift curves. The hydrolysis rate of CMC under different pH conditions at 30 degrees C showed that cellulase had high hydrolysis ability approximately at pH 5.0. Kinetic parameters (the Michaelis constant K(m) and the maximum rate V(max)) of the process were estimated by using a linear method of Lineweaver-Burk plot. K(m) is 1.95+/-0.25 mg ml(-1) and V(max) is -(4.25+/-0.58) x 10(-3) g(1/2) cm(-3/2) cP(1/2) min(-1). Also the activation energy (E(a)) of the enzymatic hydrolysis, with a value of 51.99+/-1.26 kJ mol(-1), was estimated in this work.
He
D
D
College of Chemistry and Chemical Engineering, Hunan University, Changsha, People's Republic of China.
Bao
L
L
Long
Y
Y
Wei
W
W
Yao
S
S