Membrane preconcentration-capillary electrophoresis tandem mass spectrometry (mPC-CE-MS/MS) in the sequence analysis of biologically derived peptides.
We demonstrate the use of membrane preconcentration capillary electrophoresis tandem mass spectrometry (mPC-CE-MS/MS) for sequencing peptides at the sub-100 femtomole level. In particular by loading the mPC-CE cartridge off-line with a pressurized bomb apparatus, 100 mul solutions can be loaded in <5 min. Furthermore, mPC-CE-MS in conjunction with on-line transient isotachophoresis carried out in 25 mum i.d. capillaries results in enhanced resolution and theoretical plate values as compared to convention 50-75 mum i.d. capillaries. We show that this is a powerful new approach in the sequencing of biologically derived compounds from complex mixtures such as MHC class I peptides.
Naylor
S
S
Biomedical Mass Spectrometry Facility and Department of Biochemistry and Molecular Biology, Guggenheim CL C009B, Mayo Clinic/Foundation, Rochester, MN 55905, USA; Department of Pharmacology and Clinical Pharmacology Unit, Guggenheim CL C009B, Mayo Clinic/Foundation, Rochester, MN 55905, USA.
Tomlinson
A J
AJ