title: Comparison of three enzyme-linked immunosorbent assays to detect Porcine circovirus-2 (PCV-2)-specific antibodies after vaccination or inoculation of pigs with distinct PCV-1 or PCV-2 isolates.
abstract: Porcine circovirus-2 (PCV-2) serology is frequently used to determine PCV-2 status and optimal timing of PCV-2 vaccination in the field. The objectives of the current study are to determine the diagnostic accuracy of 3 currently available commercial anti-immunoglobulin G (IgG) PCV-2 enzyme-linked immunosorbent assays (ELISAs) and to compare the ability of the 3 assays to detect and differentiate between anti-PCV-2a and anti-PCV-2b antibodies, as well as anti-PCV-2 and anti-PCV-1 antibodies. Fifty-five 3-week-old, conventional pigs were randomly allocated to 7 groups: 1) negative controls (n = 7), 2) PCV-2a (n = 8; inoculated with PCV-2 ISU-40895), 3) PCV-2b (n = 8; inoculated with PCV-2 NC-16845), 4) PCV-1 (n = 8), 5) vaccine A (n = 8; Ingelvac CircoFLEX), 6) vaccine B (n = 8; Circumvent PCV2), and 7) vaccine C (n = 8; Suvaxyn PCV2 One Dose). Blood samples were collected weekly, and all sera were tested by 3 different anti-PCV-2 IgG ELISAs. The results indicated that all ELISAs had area under the receiver operating curve values greater than 0.94, detected both anti-PCV-2a and -2b antibodies with no differentiation, and did not detect anti-PCV-1 antibodies in infected animals. One of the ELISAs was able to distinguish pigs vaccinated with vaccine B from pigs inoculated with either PCV-2a or PCV-2b.
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA.
authors.forename: Abby R
authors.initials: AR
authors.lastname: Patterson
authors.forename: John
authors.initials: J
authors.lastname: Johnson
authors.forename: Sheela
authors.initials: S
authors.lastname: Ramamoorthy
authors.forename: Xiang-Jin
authors.initials: XJ
authors.lastname: Meng
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Halbur
authors.forename: Tanja
authors.initials: T
authors.lastname: Opriessnig
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D018173:Circoviridae Infections / Q000175:diagnosis / Q000276:immunology* / Q000662:veterinary*; D017925:Circovirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D016366:Open Reading Frames; D015203:Reproducibility of Results; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology*; D014611:Vaccination / Q000379:methods / Q000662:veterinary; D014765:Viral Vaccines / Q000008:administration & dosage*
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines