[The effects of FRNK on expressions of MMP-2 mRNA and TIMP-2 mRNA in hepatic stellate cells].
OBJECTIVE
To investigate the effects of FAK-related non-kinase (FRNK) on expressions of type I collagen and matrix metalloproteinase-2 (MMP-2) mRNA and tissue inhibitor of metalloproteinase-2 (TIMP-2) mRNA in rat hepatic stellate cells (HSC).


METHODS
Using in vitro cell culture technique, FRNK plasmids were transfected into HSC mediated by cationic liposome. Type I collagen synthesis capability in HSC was examined by 3H-Pro incorporation assay. The levels of FRNK in HSC were assayed by Western blot, and the expressions of MMP-2 and TIMP-2 were assayed by RT-PCR on mRNA levels.


RESULTS
The exposure of HSC to FRNK caused the expression of FRNK protein to be up-regulated, and the FRNK protein contents reached the highest point at 48 h after the transfection, P less than 0.05. The expressions of MMP-2 mRNA were up-regulated by FRNK; the expressions of TIMP-2 mRNA were down-regulated by FRNK; the ratios of MMP-2 mRNA/TIMP-2 mRNA were enhanced by FRNK.


CONCLUSIONS
After FRNK was transfected, the capability of type I collagen synthesis in HSC was inhibited, which may be related to the up-regulation of MMP-2 mRNA/TIMP-2 mRNA.
Wei
Juan
J
Department of Gastroenterology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Zhang
Xiao-lan
XL
Yao
Dong-mei
DM
Huo
Xiao-xia
XX
Shen
Jian-gang
JG
Dun
Zhi-na
ZN
D000818:Animals; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D010957:Plasmids; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism*; D014162:Transfection
D024042:Collagen Type I; D012333:RNA, Messenger; D019716:Tissue Inhibitor of Metalloproteinase-2; C113183:FAK-related nonkinase; D011505:Protein-Tyrosine Kinases; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat