Tartrate-resistant acid phosphatase activity and glutathione levels are modulated during hFOB 1.19 osteoblastic differentiation.
Tartrate-resistant acid phosphatase (TRAP) is a well-known marker of osteoclasts and bone resorption. Here we have investigated whether osteoblast-like cells (hFOB 1.19) present TRAP activity and how would be its pattern of expression during osteoblastic differentiation. We also observed how the osteoblastic differentiation affected the reduced glutathione levels. TRAP activity was measured using the p-nitrophenylphosphate substrate. The osteogenic potential of hFOB 1.19 cells was studied by measuring alkaline phosphatase activity and mineralized nodule formation. Oxidative stress was determined by HPLC and DNTB assays. TRAP activity and the reduced glutathione-dependent microenvironment were modulated during osteoblastic differentiation. During this phase, TRAP activity, as well as alkaline phosphatase and glutathione increased progressively up to the 21st day, decreasing thereafter. We demonstrate that TRAP activity is modulated during osteoblastic differentiation, possibly in response to the redox state of the cell, since it seemed to depend on suitable levels of reduced glutathione.
de Souza Malaspina
Tatiana Salles
TS
Department of Biological Sciences, Bauru Dental School-University of São Paulo, Bauru, SP, Brazil.
dos Santos
Célio Xavier
CX
Campanelli
Ana Paula
AP
Laurindo
Francisco Rafael Martins
FR
Sogayar
Mari Cleide
MC
Granjeiro
José Mauro
JM
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D002113:Calcification, Physiologic; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D018384:Oxidative Stress; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D005978:Glutathione