Topical anti-inflammatory activity of dianemycin isolated fromStreptomyces sp. MT 2705-4.
In order to develop new anti-inflammatory agents having different action mechanisms compared with nonsteroidal and steroidal anti-inflammatory drugs, the culture broths of various actinomycetes isolated from soil were screened using anin vivo mouse ear edma assay and one strain (Streptomyces sp. MT 2705-4: KCTC 8651P) was selected. Activity-guided purification led to the isolation of a polyether compound, dianemycin. Topically, dianemycin showed a potent anti-inflammatory activity in mouse ear edema induced by croton-oil or arachidonic acid. ED(50) value of dianemycin was found to be 0.8 mg/ear compared to 0.4 mg/ear of prednisolone in croton-oil ear edema. However, dianemycin did not show the inhibitory activity in UV-erythema and delayed hypersensitivity reaction. These results indicate that dianemycin is a potential topical anti-inflammatory agent.
Lee
S J
SJ
College of Pharmacy, Kangwon National University, 200-701, Chuncheon, Korea.
Kim
H P
HP
Park
B K
BK
Ahn
S C
SC
Lee
H S
HS
Ahn
J S
JS