title: Surface-grafting of phosphates onto a polymer for potential biomimetic functionalization of biomaterials.
abstract: In the human body, phosphate groups play important roles in signaling and the biological functions of proteins and peptides. Despite the importance of phosphate groups, polymer surfaces have not been directly grafted with phosphate groups by chemical reactions because the usual organic solvents used to graft phosphate groups can dissolve or swell polymers. We focused this study on grafting phosphate groups onto a poly(ethylene-co-acrylic acid) (PEAA) surface in an aqueous solution. O-phospho L-serine and O-phosphoethanolamine were grafted on PEAA surfaces to introduce phosphate groups by activating carboxylic acid groups of PEAA using N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in an aqueous environment. X-ray photoelectron spectroscopy (XPS) was used to elucidate the process by which surface grafting occurs and the process that the phosphate group is cleaved into a phosphate ion and a hydrolyzed molecule at high pH. It was found that under appropriate reaction conditions the phosphate groups could be successfully grafted on the polymer surfaces. The phosphate-grafted polymer surfaces showed lower water contact angles than the initial polymer surfaces likely due to their highly mobile and hydrophilic phosphate side groups. This work demonstrates a technique to successfully graft phosphate groups onto organic polymer surfaces in a biocompatible aqueous environment, which may open new avenues to functionalizing synthetic polymeric and natural macromolecule derived biomaterials.
authors.affiliation: Department of Biologic and Materials Sciences, University of Michigan, 1011 North University Ave., Ann Arbor, MI 48109-1078, USA.
authors.forename: Young Gun
authors.initials: YG
authors.lastname: Ko
authors.forename: Peter X
authors.initials: PX
authors.lastname: Ma
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis; D040761:Biomimetic Materials / Q000138:chemical synthesis*; D010710:Phosphates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D010710:Phosphates; D011108:Polymers