title: Preparation and application of functionalized nanoparticles of CdSe capped with 11-mercaptoundecanoic acid as a fluorescence probe.
abstract: A new fluorescence probe, CdSe nanocrystal has been prepared and modified with 11-mercaptoundecanoic acid [HS-(CH(2))(10)-COOH]. The functionalized nanoparticles were characterized using transmission electron microscopy (TEM), ultraviolet-visible (UV-vis) spectroscopy and photoluminescence (PL) spectroscopy. The results demonstrate that CdSe is dispersed homogeneously in aqueous solution and well protected from the environmental oxygen. They can be used as a new fluorescence probe for lysozyme, which was simple, rapid and specific. Under the optimum condition, the response is linearly proportional to the amount of lysozyme from 0.20 to 26.0mugml(-1), and the limit of detection is 0.115mugml(-1). The proposed method has been applied to the determination of lysozyme in egg white, with the recovery of 96-105%.
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhong
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Lai
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Long
authors.forename: Chunsui
authors.initials: C
authors.lastname: Liang