title: Direct electrochemistry and superficial characterization of DNA-cytochrome c-MUA films on chemically modified gold surface.
abstract: Cytochrome c (Cyt. c) was immobilized on the 11-mercaptohendecanoic acid (MUA)-modified gold electrode. The electrode was stable and sensitive to Cyt. c. Later, DNA was also immobilized on the two-layer modified electrode. Cyclic voltammetry studies show that Cyt. c can interact with dsDNA and ssDNA. The binding site sizes were determined to be 15 base pairs per Cyt. c molecule with dsDNA and 30 nucleotides binding 1 Cyt. c molecule with ssDNA. The modified electrodes were characterized by quartz crystal microbalance (QCM), impedance spectroscopy and atomic force microscope (AFM). The modified electrode can be used for determining DNA.
authors.affiliation: Department of Chemistry, Beijing Normal University, Beijing, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Ding
authors.forename: Jingbo
authors.initials: J
authors.lastname: Hu
authors.forename: Qilong
authors.initials: Q
authors.lastname: Li