title: Local inflammation exacerbates cyclosporine a-induced gingival overgrowth in rats.
abstract: Gingival overgrowth (GO) is a common side effect of long-term cyclosporine A (CsA) treatment. The risk factors appraised include drug interactions with calcium channel blockers, age, cyclosporine dose, dental bacterial plaque, duration of treatment, and genetic predisposition. The relationship and mechanism between GO and local inflammation caused by dental bacterial plaque have not been clearly defined. This research was carried out to investigate the histomorphometrical alterations and serum levels of transforming growth factor beta1 (TGF-beta1) in CsA-induced GO with or without local inflammation. Thirty-four male Sprague-Dawley rats were divided into 4 groups: Group I (control); Group II (ligation); Group III (CsA); Group IV (ligation and CsA). After 9 weeks the rats were sacrificed. The morphological examination was made and the histological changes with hematoxylin and eosin (HE) staining were observed. TGF-beta1 levels in serum were detected by enzyme-linked immunosorbent assay (ELISA). We report here that obvious GO are found in Group III and Group IV after CsA treatment, especially those rats with existed gingivitis presented an aggravation of GO. TGF-beta1 levels in CsA-exposed groups were significantly higher than untreated groups, but ligation did not affect TGF-beta1 level. These findings suggest that CsA-induced GO can be exacerbated by local inflammation. TGF-beta1 may be a key factor for the development of GO.
authors.affiliation: School and Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Zhong Road, Fuzhou, Fujian 350002, People's Republic of China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Guo
authors.forename: Wenyu
authors.initials: W
authors.lastname: Wang
authors.forename: Liyan
authors.initials: L
authors.lastname: Yao
authors.forename: Fuhua
authors.initials: F
authors.lastname: Yan
mesh_terms: D000818:Animals; D001835:Body Weight; D016572:Cyclosporine; D005881:Gingiva / Q000378:metabolism / Q000473:pathology*; D019214:Gingival Overgrowth / Q000097:blood / Q000139:chemically induced / Q000209:etiology* / Q000473:pathology; D005891:Gingivitis / Q000097:blood / Q000150:complications* / Q000473:pathology; D008026:Ligation; D008297:Male; D008964:Molar, Third / Q000601:surgery; D009969:Orthodontic Wires; D051381:Rats; D017207:Rats, Sprague-Dawley; D012307:Risk Factors; D053773:Transforming Growth Factor beta1 / Q000097:blood
chemical_list: D053773:Transforming Growth Factor beta1; D016572:Cyclosporine