An integrated light emitting diode-induced fluorescence detector for capillary electrophoresis.
An integrated light emitting diode (LED)-induced fluorescence detector was described and evaluated. The LED and its related components including lens and interference filter, the optical fiber used to collect fluorescence, and the capillary column are integrated into a substrate block, which eliminates the need of align procedure of the fiber and the capillary. Forty-fold enhancement of sensitivity was obtained compared with our previous work and the detection limit for fluorescein was 5nM. Application of the detector for the analysis of FITC-labeled Ephedrine extract was demonstrated.
Yang
Bingcheng
B
Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Tian
Hongzhe
H
Xu
Jing
J
Guan
Yafeng
Y