A facile and sensitive spectrophotometric determination of ascorbic acid.
A simple and sensitive procedure for the spectrophotometric determination of ascorbic acid is described. It involves formation of ferroin when ascorbic acid reacts with a mixture of iron(III) and 1,10-phenanthroline, and measurement of the absorbance at 510 nm. The procedure has been applied to assay of pharmaceutical preparations of vitamin C. The average error did not exceed 0.3%.
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Microanalytical Research Laboratory, National Research Centre, Dokki, Cairo, Egypt.