Bovine serum albumin as a means to immobilize DNA on a silver-plated bulk acoustic wave DNA biosensor.
A simple, inexpensive, on-line bulk acoustic wave (BAW) DNA biosensor is proposed by using an Ag-plated surface rather than the conventional Au surface. Bovine serum albumin (BSA) is used as an active coating for DNA immobilization. Impedance analysis reveals that the film composed of didodecyl dithiono-oxamide (DDDTO) and BSA can dramatically enhance the amount of immobilized DNA. Detection of dissociation and hybridization of immobilized DNA is demonstrated as an example of the potential application of this type of acoustic wave DNA biosensor in clinical practice.
Zhang
H
H
New Material Research Institute, Chemistry and Chemical Engineering College, Hunan University, Changsha, 410082, People's Republic of China.
Wang
R
R
Tan
H
H
Nie
L
L
Yao
S
S