Fluorescent reaction between ascorbic acid and DAN and its analytical application.
Fluorescent reaction between ascorbic acid (AA) and 2,3-diamino-naphthalene (DAN) was studied. The experimental results showed that AA could react with DAN at pH = 10.2-10.5, and form the fluorescent heterocyclic condensation products which emitted strong fluorescence. The fluorescence intensity was measured in a 1 cm quartz cell with excitation and emission wavelengths of 400 and 520 nm, respectively. The relationship was obtained between the fluorescence intensity and AA concentration in the range of 2-300 mug ml(-1), the regression coefficient is 0.9993. The detection limit (signal-to-noise = 2) is 0.4 mug ml(-1).
Yang
J
J
Department of Chemistry, Shandong University, Jinan 250100, China.
Tong
C
C
Jie
N
N
Zhang
G
G
Ren
X
X
Hu
J
J