TLR4 and IL-18 gene variants in aggressive periodontitis.
OBJECTIVE
We aimed to assess the association of different genotypes with increased aggressive periodontitis susceptibility by studying functional relevant variants in the pathogen-recognition receptor Toll-like receptor 4 (TLR4) and variants in the promoter region of the pro-inflammatory cytokine interleukin-18 (IL-18).


METHODS
One hundred and eleven patients with aggressive periodontitis and 80 periodontally healthy controls were genotyped for four functional variants in the TLR4 gene (c.896A>G and c.1196C>T) and in the IL-18 promoter (c.-368G>C and c.-838C>A). The genotype and allele frequencies, as well as the frequency of combined genotypes were compared between study groups.


RESULTS
There were no statistical differences in genotype and allele frequencies within the four variants between the groups. All study subjects were further classified into carriers and non-carriers of at least one variant of both genes. The logistic regression analysis adjusted for gender and smoking showed no association between carrier status of at least one variant of both genes and periodontal status (OR=1.41, 95% CI: 0.43-4.70).


CONCLUSIONS
Our results reject the hypothesis that functionally relevant IL-18 and TLR4 gene mutations have a major effect on aggressive periodontitis susceptibility alone or in combination.
Noack
Barbara
B
Department of Conservative Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Germany. Barbara.Noack@uniklinikum-dresden.de
Görgens
Heike
H
Lorenz
Katrin
K
Ziegler
Andreas
A
Hoffmann
Thomas
T
Schackert
Hans Konrad
HK
D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D044465:White People
D020382:Interleukin-18; D051197:Toll-Like Receptor 4