Quantitative analysis of protein-RNA interactions by gel mobility shift.
The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to resolve free from bound RNA with high resolution in a gel matrix. We review the quantitative application of this approach to elucidate thermodynamic properties of protein-RNA complexes. Assay designs for titration, competition, and stoichiometry experiments are presented for two unrelated model complexes.
Ryder
Sean P
SP
Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Worcester, Massachusetts, USA.
Recht
Michael I
MI
Williamson
James R
JR
D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism
D011506:Proteins; D012313:RNA