title: Validated reversed-phase column high-performance liquid chromatographic method for separation and quantification of polyphenolics and furocoumarins in herbal drugs.
abstract: A rapid column high-performance liquid chromatographic-photodiode array method has been developed for the separation and identification of secondary metabolites, especially different types of phenols and furocoumarins, in a 35 min chromatographic run. The method has been optimized and validated for selectivity, precision, recovery, and robustness with the aim of application for standardization of selected herbal drugs. Almost all of the tested compounds had linearity of >98%, with relative standard deviation <10% in terms of variation of retention time. Interday and intraday variability was <5%. The developed method has been successfully applied in identification and quantification of phenols and furocoumarins present in different plants, viz., Artemisia pallens (whole plant), Hibiscus rosa-sinensis DC (flower), Heracleum candicans DC (fruit), and Ficus carica Linn (bark). The results indicate that the method is rapid, accurate, and robust for the analysis of different types of phenols and furocoumarins and, hence, can be successfully used in the quality control and standardization of plant extracts and herbal drugs.
authors.affiliation: National Botanical Research Institute, Pharmacognosy and Ethnopharmacology Division, Lucknow, India. pharmacognosy1@rediffmail.com
authors.forename: Raghavan
authors.initials: R
authors.lastname: Govindarajan
authors.forename: Dhirendra Pratap
authors.initials: DP
authors.lastname: Singh
authors.forename: Ajay Kumar Singh
authors.initials: AK
authors.lastname: Rawat
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D035264:Flowers / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D011564:Furocoumarins / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis; D028321:Plant Preparations / Q000032:analysis*; D010944:Plants / Q000737:chemistry; D059808:Polyphenols; D015203:Reproducibility of Results
chemical_list: D005419:Flavonoids; D011564:Furocoumarins; D010636:Phenols; D010936:Plant Extracts; D028321:Plant Preparations; D059808:Polyphenols