Constant Calcein Blue: An indicator for spectrofluorometric calcium determination.
A new fluorescent indicator for metal ions, called Constant Calcein Blue, has been synthesized by condensing 7-hydroxy-2,3-dimethylchromone, iminodiacetic acid and formaldehyde. The indicator exhibits extraordinary resistance to alkaline hydrolysis and is recommended for spectrofluorometric determinations of metal ions, such as calcium, that must be performed at pH 10.5 and higher.
Huitink
G M
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Department of Chemistry, Indiana University at South Bend, South Bend, Indiana 46615, U.S.A.