title: [Dynamic determination of sinomenine in skin by microdialysis].
abstract: OBJECTIVE
  To explore the feasibility and advantages of using microdialysis as sampling method for dynamic determination of sinomenine in topical skin.
  
  
  METHODS
  In this study, sinomenine was administered to the rats by transdermal drug delivery system and celiac injection. With microdialysis technique for sampling, the concentration of sinomenine in dialysate was determined by high performance liquid chromatography (HPLC).
  
  
  RESULTS
  Under existing determination condition, topical drug concentration in the skin of rats was hard to be detected after sinomenine administered to the skin, but it could be detected after celiac injection.
  
  
  CONCLUSIONS
  Microdialysis sampling method can be used to determine topical drug concentration in the skin dynamically, and this method is better than traditional methods obviously.
authors.affiliation: College of Chinese Drug, Guangzhou University of TCM, Guangzhou 510006, China. ljj@gahtcm.edu.cn
authors.forename: Jia-Jun
authors.initials: JJ
authors.lastname: Ling
authors.forename: Bo
authors.initials: B
authors.lastname: Xie
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Li-Ling
authors.initials: LL
authors.lastname: Zhou
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005240:Feasibility Studies; D007274:Injections, Intraperitoneal; D008297:Male; D029583:Menispermaceae / Q000737:chemistry*; D017551:Microdialysis / Q000379:methods*; D009019:Morphinans / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism*
chemical_list: D009019:Morphinans; C009271:sinomenine