Clonazepam release from core-shell type nanoparticlesin vitro.
AB-type amphiphilic copolymers (abbreviated as LE) composed of poly (L-leucine) (PLL) as the A component and poly (ethylene oxide) (PEO) as the B component were synthesized by the ring-opening polymerization of L-leucine N-carboxy-anhydride initiated by methoxy polyoxyethylene amine (Me-PEO-NH(2)) and characterized. Core-shell type nanoparticles were prepared by the diafiltration method. Particle size distribution obtained by dynamic light scattering was dependent on PLL composition and the size for LE-1, LE-2 and LE-3 was 369.6+/-267, 523.4+/-410 and 561.2+/-364 nm, respectively. Shapes of the nanoparticles observed by transmission electron microscope (TEM) were almostly spherical. The critical micelle concentration (CMC) of the nanoparticles determined by a fluorescence probe technique was dependent on the composition of hydrophobic PLL, and the CMC for LE-1, LE-2 and LE-3 was 2. 0x10(-6), 1.7x10(-6) and 1.5x10(-6) (mol/l), respectively. Clonazepam release from core-shell type nanoparticles in vitro was dependent on PLL composition and drug loading content.
Kim
H J
HJ
Department of Polymer Engineering, Chonnam National University, 500-757, Kwangju, Korea.
Jeong
Y I
YI
Kim
S H
SH
Lee
Y M
YM
Cho
C S
CS