Quantitative evaluation of thin-layer chromatograms.
A commercially available scanning photometer, designed to scan paper electrophoresis strips, has been used to evaluate thinlayer chromatograms. The chromatogram may be lifted off the glass plate with cellophane tape which is then cut into strips and scanned, or the thin-layer chromatogram may be photographed and the photograph scanned. Newly developed cellulose-backed thin layers of silica or alumina may also be cut into strips and scanned. The instrument has also been modified to scan glass plates. A correction is applied for non-uniformity of the thin layer. Zones containing colourless components can be located by means of an auxiliary chromatogram, and the components present determined by carbon analysis.
Keyworth
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Universal Oil Products Company, Des Plaines, Illinois, U.S.A.
Swensen
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