title: The effect of iNOS inhibitors and hyperbaric oxygen treatment in a rat model of experimental colitis.
abstract: OBJECTIVE
  Our aim was to investigate the effectiveness of aminoguanidine (AMG), an inducible nitric oxide synthase inhibitor, and hyperbaric oxygen (HBO) treatment in an experimental colitis model.
  
  
  METHODS
  We induced colitis in rats. In the control group, we applied 2 ml serum physiologic intraperitoneally for 7 days. In the HBO group, 100% oxygen at 2.4 atm pressure was applied for 7 days. In the AMG group, 100 mg/kg AMG was applied intraperitoneally for 7 days. In the HBO + AMG group, HBO and AMG were applied, respectively. At the end of 7 days, rats were sacrificed and the distal 10 cm part of colon was examined macro- and microscopically.
  
  
  RESULTS
  Severity of colitis and NO activities were reduced by AMG, HBO, and HBO + AMG application. There was histologically significant improvement, especially in the HBO + AMG group.
  
  
  CONCLUSIONS
  Both HBO and AMG were significantly effective in preventing weight loss, reducing NO activities, and severity of colitis, when comparing HBO and AMG separately.
authors.affiliation: Division of Internal Medicine, Department of Gastroenterology, Gulhane School of Medicine, Etlik, Ankara, Turkey. cnercin@hotmail.com
authors.forename: Cemal Nuri
authors.initials: CN
authors.lastname: Ercin
authors.forename: Zeki
authors.initials: Z
authors.lastname: Yesilova
authors.forename: Ahmet
authors.initials: A
authors.lastname: Korkmaz
authors.forename: Ayhan
authors.initials: A
authors.lastname: Ozcan
authors.forename: Cagatay
authors.initials: C
authors.lastname: Oktenli
authors.forename: Ahmet
authors.initials: A
authors.lastname: Uygun
mesh_terms: D019342:Acetic Acid; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000628:therapy*; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D005260:Female; D006146:Guanidines / Q000627:therapeutic use*; D006931:Hyperbaric Oxygenation / Q000379:methods*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D016896:Treatment Outcome
chemical_list: D006146:Guanidines; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D019342:Acetic Acid; C004479:pimagedine