Chemiluminescent evaluation of peroxide value in olive oil.
A method is described for the evaluation of the peroxide value (PV, meq. O(2) kg(-1)) in olive oil. The method is based on the chemiluminogenic energy-transfer reaction of bis(2,4,6-(trichlorophenyl)oxalate (TCPO) with hydrogen peroxide or total peroxides in the presence of Mn(II) as catalyst and 9,10-dimethylanthracene as fluorophore. The procedure developed allows the evaluation of PV within the range of 0.6-100meq. O(2) kg(-1) (CL intensity = 1.76 x PV (meq. O(2) kg(-1)) + 23.2, r(2) = 0.994, n = 9) and relative standard deviation within the range 1-5% by using a simple manual measurement.
Stepanyan
Vahan
V
Mediterranean Agronomic Institute of Chania, P.O. Box 85, 73100 Chania, Crete, Greece.
Arnous
Anis
A
Petrakis
Christos
C
Kefalas
Panagiotis
P
Calokerinos
Antony
A