N-glycan trimming by glucosidase II is essential for Arabidopsis development.
Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble heterodimer composed of alpha and beta subunits. Here we present the first characterization of a plant glucosidase II alpha subunit at the molecular level. Expression of the Arabidopsis alpha subunit restored N-glycan maturation capacity in Schizosaccharomyces pombe alpha- or alphabeta-deficient mutants, but with a lower efficiency in the last case. Inactivation of the alpha subunit in a temperature sensitive Arabidopsis mutant blocked N-glycan processing after a first trimming by glucosidase I and strongly affected seedling development.
Soussilane
Pravina
P
CNRS, UMR 6037, IFRMP 23, Bâtiment Biologie Extension, Faculté des Sciences, Mont-Saint-Aignan, France.
Soussillane
Pravina
P
D'Alessio
Cecilia
C
Paccalet
Thomas
T
Fitchette
Anne-Catherine
AC
Parodi
Armando J
AJ
Williamson
Richard
R
Plasson
Carole
C
Faye
Loïc
L
Gomord
Véronique
V
D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D036226:Seedlings / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism*
D029681:Arabidopsis Proteins; D011134:Polysaccharides; C034251:4-nitrophenyl-alpha-glucosidase; D000520:alpha-Glucosidases