The MIRA method for DNA methylation analysis.
DNA methylation patterns are often altered in human cancer and aberrant methylation is considered a hallmark of malignant transformation. Several methods have been developed for the characterization of gene-specific and genome-wide DNA methylation patterns. In this chapter, we describe the methylated-CpG island recovery assay (MIRA), which is based on the high affinity of the MBD2b/MBD3L1 complex for double-stranded CpG-methylated DNA. MIRA has been used in combination with microarray platforms to map DNA methylation patterns across the human genome.
Rauch
Tibor A
TA
Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA.
Pfeifer
Gerd P
GP
D018899:CpG Islands; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D019175:DNA Methylation; D004268:DNA-Binding Proteins; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins
D004268:DNA-Binding Proteins; C115368:MBD2 protein; C474631:MBD3L1 protein, human; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D004247:DNA