title: No antioxidant beneficial effect of zinc supplementation on oxidative stress markers and antioxidant defenses in middle-aged and elderly subjects: the Zenith study.
abstract: OBJECTIVE
  The aim of the study was to investigate whether zinc supplementation affects antioxidant status in European middle-aged and elderly people.
  
  
  METHODS
  Multicentre prospective intervention study, randomized, double-blind, placebo-control.
  
  
  METHODS
  France (Clermont-Ferrand/Theix, and Grenoble), Italy (Rome), Northern Ireland (Coleraine).
  
  
  METHODS
  A total of 387 healthy middle-aged (55-70 yrs) and free-living older aged (70-85 yrs) subjects were randomly allocated to three groups: 0, 15 or 30 mg zinc gluconate/d in addition to usual dietary intake during 6 months.
  
  
  METHODS
  Oxidative stress status was evaluated by measurement of protein oxidation (plasma thiol groups), lipid peroxidation (plasma thio-barbituric acid reactants, TBARS), whole blood glutathione levels, erythrocyte copper/zinc superoxide dismutase activity and plasma antioxidant status (ferric reducing antioxidant power assay), at baseline and after 3 and 6 months.
  
  
  RESULTS
  Zinc supplementation did not alter oxidative stress markers and antioxidant defenses in elderly, after 3 or 6 months, except an increase in Cu/Zn superoxide dismutase activity.
  
  
  CONCLUSIONS
  In apparently healthy free living elderly people, a single zinc supplementation had no effects on oxidative stress status.
authors.affiliation: INSERM and LBFA, Université J. Fourier, Grenoble, France.
authors.forename: Maud
authors.initials: M
authors.lastname: Andriollo-Sanchez
authors.forename: Isabelle
authors.initials: I
authors.lastname: Hininger-Favier
authors.forename: Nathalie
authors.initials: N
authors.lastname: Meunier
authors.forename: Eugenia
authors.initials: E
authors.lastname: Venneria
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: O'Connor
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Maiani
authors.forename: Angela
authors.initials: A
authors.lastname: Polito
authors.forename: Séverine
authors.initials: S
authors.lastname: Bord
authors.forename: Monique
authors.initials: M
authors.lastname: Ferry
authors.forename: Charles
authors.initials: C
authors.lastname: Coudray
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Roussel
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D015415:Biomarkers / Q000378:metabolism; D003404:Creatinine / Q000652:urine; D019587:Dietary Supplements; D004311:Double-Blind Method; D006801:Humans; D028421:Isoprostanes / Q000652:urine; D015227:Lipid Peroxidation / Q000187:drug effects; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D028421:Isoprostanes; D003404:Creatinine; D013482:Superoxide Dismutase; D015032:Zinc