[Genetic polymorphism analysis of 3 short tandem repeat loci on chromosome 21 in Chinese Han population in Shaanxi Province].
OBJECTIVE
To investigate the polymorphism of 3 short tandem repeat (STR) loci D21S1442, D21S1413 and D21S1809 on human chromosome 21 in Shaanxi Han nationality in China.


METHODS
Blood samples anti-coagulated with EDTA were collected from 160 unrelated Chinese Han individuals in Shaanxi Province. The genomic DNA was extracted with Chelex-100, amplified by PCR and analyzed using PAGE with silver staining.


RESULTS
Nine, eight and seven alleles were detected at the loci of D21S1442, D21S1413 and D21S1809, respectively. The genotype distributions of all the 3 loci were in accordance with Hardy-Weinberg equilibrium. The heterozygosities of the 3 loci were 0.750, 0.742 and 0.694, respectively. No linkage disequilibrium was found between the loci.


CONCLUSIONS
The 3 loci on chromosome 21 have high heterozygosity and polymorphic information content in Shaanxi Han population, suggesting their value as potentially useful genetic markers.
Wang
Xiang
X
Center for Laboratory Medicine, First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. wx6212@yahoo.cn
Chen
Wei
W
Zhao
Le
L
Zhou
Ping
P
Li
Xu
X
D044466:Asian People / Q000208:ethnology / Q000235:genetics; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*
D005819:Genetic Markers