Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells.
The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 x 10(5) cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.
Yoon
Jong Hyun
JH
Department of Laboratory Medicine, Seoul National University Boramae Hospital, 425 Shindaebang-dong, Dongjak-gu, Seoul, 156-707, South Korea.
Roh
Eun Youn
EY
Shin
Sue
S
Jung
Nam Hee
NH
Song
Eun Young
EY
Lee
Dong Soon
DS
Han
Kyou Sup
KS
Kim
Joung Sung
JS
Kim
Byoung Jae
BJ
Jeon
Hye Won
HW
Yoon
Kang Sup
KS
D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D059630:Mesenchymal Stem Cells; D014465:Ultrasonics; D014470:Umbilical Cord / Q000166:cytology*