title: Immobilization and controlled release of prostaglandin E2 from poly-L-lactide-co-glycolide microspheres.
abstract: Prostaglandin E(2) (PGE(2)) is an arachidonic acid metabolite involved in physiological homeostasis and numerous pathophysiological conditions. Furthermore, it has been demonstrated that prostaglandins have a stimulating effect not only on angiogenesis in situ and in vitro but also on chondrocyte proliferation in vitro. Thus, PGE(2) represents an interesting signaling molecule for various tissue engineering strategies. However, under physiological conditions, PGE(2) has a half-life time of only 10 min, which limits its use in biomedical applications. In the present study, we investigated if the incorporation of PGE(2) into biodegradable poly-L-lactide-co-glycolide microspheres results in a prolonged release of this molecule in its active form. PGE(2)-modified microspheres were produced by a cosolvent emulsification method using CHCl(3) and HFIP as organic solvents and PVA as emulsifier. Thirteen identical batches were produced; and to each batch 1.0 mL of serum-free medium was added. The medium was removed at defined time points and then analyzed by gas chromatography tandem mass spectrometry (GC/MS/MS) to measure the residual PGE(2) content. In this study we demonstrated the prolonged release of PGE(2), showing a linear increase over the first 12 h, followed by a plateau and a slow decrease. The microspheres were further characterized by scanning electron microscopy.
authors.affiliation: REPAIR-lab, Institute of Pathology, Johannes Gutenberg University, Langenbeckstrasse 1, Mainz, Germany. brochhausen@pathologie.klinik.uni-mainz.de
authors.forename: Christoph
authors.initials: C
authors.lastname: Brochhausen
authors.forename: Rolf
authors.initials: R
authors.lastname: Zehbe
authors.forename: Bernhard
authors.initials: B
authors.lastname: Watzer
authors.forename: Sven
authors.initials: S
authors.lastname: Halstenberg
authors.forename: Franziska
authors.initials: F
authors.lastname: Gabler
authors.forename: Helmut
authors.initials: H
authors.lastname: Schubert
authors.forename: Charles James
authors.initials: CJ
authors.lastname: Kirkpatrick
mesh_terms: D003692:Delayed-Action Preparations / Q000737:chemistry*; D015232:Dinoprostone / Q000008:administration & dosage*; D007700:Kinetics; D008863:Microspheres; D010316:Particle Size; D011098:Polyglactin 910 / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D011098:Polyglactin 910; D015232:Dinoprostone