Reversed-phase liquid chromatography of alkaloids: Masking effects of inorganic salts.
The addition of salts (investigated cations Na(+), NH(+)(4), K(+), KBu(+)(4) combined with the anions acetate, Br(-), SCN(-) and I(-)) can be used to eliminate peak tailing and to decrease retention of cationic species in the course of the separation of alkaloids from Chelidonium majus L. by reversed-phase chromatography on a ODS Hypersil column, using water-acetonitrile-methanol mixture as eluent. These findings are interpreted in terms of a silanol masking effect. The extended donor-acceptor concept is used to interpret the effectiveness of different salts in masking the active sites of the stationary phase.
Nowicky
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Institute of Inorganic Chemistry, Technical University of Vienna, Getreidemarkt 9, A-1060 Vienna, Austria.
Han
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Nowicky
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Gutmann
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Linert
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