title: Biophysical analyses of human resistin: oligomer formation suggests novel biological function.
abstract: Resistin, a small secreted peptide initially identified as a link between obesity and diabetes in mice, was shown to be involved in mediating inflammation in humans. We had shown earlier that recombinant human resistin has a tendency to form aggregates by formation of inter/intramolecular disulfide linkages and that it undergoes a concentration-dependent conformational change in secondary structure from alpha-helical to beta-sheet form. Here we report that this change in secondary structural conformation is due to the increase in the oligomeric form of human resistin as a function of protein concentration. Gel filtration analysis under different conditions further demonstrated that recombinant human resistin exists as a mixture of oligomer and trimer but is converted to a mixture of monomer and oligomer in the presence of 100 mM NaCl. We show that while the trimeric form of human resistin is stable to urea-induced denaturation, it is highly susceptible to NaCl and NaF, indicating the importance of ionic interactions in stabilization of trimer. In addition, urea was able to destabilize the oligomers indicating the involvement of hydrophobic interactions in oligomerization. Ionic as well as hydrophobic interactions stabilize the monomeric human resistin. Our data suggest that human resistin exists predominantly as oligomer and trimer in vitro. The oligomeric form of human resistin shows more potent effect on stimulation of proinflammatory cytokines. Therefore, it is very tempting to propose that the structural conformation of resistin may be involved in maintaining the very fine balance in regulation of macrophage function for successful response to a variety of pathological conditions.
authors.affiliation: National Institute of Nutrition, Indian Council for Medical Research, Jamai Osmania, Tarnaka, Hyderabad 500007, India.
authors.forename: Battu
authors.initials: B
authors.lastname: Aruna
authors.forename: Asimul
authors.initials: A
authors.lastname: Islam
authors.forename: Sudip
authors.initials: S
authors.lastname: Ghosh
authors.forename: Anil K
authors.initials: AK
authors.lastname: Singh
authors.forename: Malladi
authors.initials: M
authors.lastname: Vijayalakshmi
authors.forename: Faizan
authors.initials: F
authors.lastname: Ahmad
authors.forename: Nasreen Z
authors.initials: NZ
authors.lastname: Ehtesham
mesh_terms: D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D011489:Protein Denaturation; D055503:Protein Multimerization; D055550:Protein Stability; D020836:Protein Structure, Quaternary; D052243:Resistin / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature
chemical_list: D052243:Resistin