Effect of diode laser on enzymatic activity of parotid glands of diabetic rats.
The aim of this study was to evaluate the effect of laser irradiation (LI) on enzymatic activities of amylase, catalase and peroxidase in the parotid glands (PG) of diabetic and non-diabetic rats. Ninety-six female rats were divided into eight groups: D0; D5; D10; D20 and C0; C5; C10; C20, respectively. Diabetes was induced by administration of streptozotocin and confirmed later by the glycemia results. Twenty-nine (29) days after the induction, the PGs of groups D5 and C5; D10 and C10; D20 and C20, were irradiated with 5 J/cm(2), 10 J/cm(2) and 20 J/cm(2) of laser diode (660 nm/100 mW) respectively. On the following day, the rats were euthanized and the enzymatic activity in the PGs was measured. Diabetic rats that had not been irradiated (group D0) showed higher catalase activity (P < 0.05) than those in group C0 (0.14 +/- 0.02 U/mg protein and 0.10 +/- 0.03 U/mg protein, respectively). However, laser irradiation of 5 J/cm(2) and 20 J/cm(2) decreased the catalase activity of the diabetic groups (D5 and D20) to non-diabetic values (P > 0.05). Based on the results of this study, LI decreased catalase activity in the PGs of diabetic rats.
Simões
Alyne
A
Oral Biology Research Center, Biomaterials and Oral Biochemistry Department, Faculty of Dentistry, University of São Paulo (USP), São Paulo, Brazil. lysimoes@usp.br
Ganzerla
Emily
E
Yamaguti
Paula Mochidome
PM
de Paula Eduardo
Carlos
C
Nicolau
José
J
D000681:Amylases / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000378:metabolism; D048909:Diabetes Complications / Q000201:enzymology / Q000209:etiology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000532:radiotherapy*; D005260:Female; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D010306:Parotid Gland / Q000201:enzymology* / Q000528:radiation effects*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014987:Xerostomia / Q000201:enzymology / Q000209:etiology / Q000532:radiotherapy
D002374:Catalase; D009195:Peroxidase; D000681:Amylases