title: Generation of infectious transcripts from Korean strain and mild mottle strain of potato virus X.
abstract: Full-length cDNAs of two different strains of Potato virus X (PVX-Kr and PVX-Mo) have been directly amplified by long template reverse transcription polymerase chain reaction (RT-PCR) using the 5'-end primer containing a SP6 or T7 RNA promoter sequence and the virus-specific 3'-end primer, and then constructed in plasmid vectors. Capped in vitro transcripts from cloned full-length cDNAs as well as those RT-PCR amplicons proved to be infectious systemically on tobacco plants. Symptom expression on tobacco plants from PVX-Mo transcripts was faster and severer than that from PVX-Kr. In replication stability test of transcripts derived from PVX clones, progeny viruses showed stable replication according to sequencing through passages. This highly infectious transcript system from the full-length cDNA clones for PVX can be useful for recombinant molecules for functional analysis of viral proteins in plant-virus interaction study as well as for expression of foreign protein in planta.
authors.affiliation: Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University, Seoul, 139-774, Republic of Korea.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Ryu
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D017863:Potexvirus / Q000235:genetics* / Q000472:pathogenicity*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014026:Nicotiana / Q000382:microbiology*; D014158:Transcription, Genetic
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral