Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material.
A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon-10(-9) mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.
Khayyami
M
M
Department of Pure and Applied Biochemistry, Centre for Chemistry and Chemical Engineering, Lund University, S-221 00 Lund, Sweden.
Pérez Pita
M T
MT
Peña Garcia
N
N
Johansson
G
G
Danielsson
B
B
Larsson
P O
PO