title: [Isolation of sperm cells of Allium tub rosum Roxb].
abstract: Pollen grains of Allium tuberosum Roxb broke and released their content including generative cells using osmotic shock method. In a medium containing 0.05% CaCl2, 0.01% Boric acid, 0.01%KH2PO4, 15%PEG 10% sucrose (710 mOsmol/kg H2O) 86% pollen grains germinated and grew out pollen tubes, which broke after transferred into 6% mannitol solution, and released tube content including generative cell. When pollen grains were cultured in the same medium but adding 0.1% casein, a few generative cells divided into two sperm cells. Stigmas of Allium tuberosum Roxb were pollinated at second day after anthesis and the styles grow 3 h in vivo. Then the styles were cut and cultured in a medium for about 6-8 h, some pollen tubes grew out of the cut end of the style. The cut end of the style was transferred into a solution containing 6% mannitol to burst pollen tubes. Pairs of sperm cells of Allium tuberosum Roxb were released and collected using a micromanipulator.
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Lv
authors.initials: L
authors.lastname: Ye
authors.forename: Dan
authors.initials: D
authors.lastname: Lv
authors.forename: Ming Xia
authors.initials: MX
authors.lastname: Jian
authors.forename: Hui Qiao
authors.initials: HQ
authors.lastname: Tian
mesh_terms: D000490:Allium / Q000166:cytology*; D011058:Pollen / Q000166:cytology*; D053205:Pollen Tube / Q000166:cytology*