title: Immobilization of uricase to gas diffusion carbon felt by electropolymerization of aniline and its application as an enzyme reactor for uric acid sensor.
abstract: Electropolymerizations of aniline and pyrrole solutions containing uricase at a neutral pH was performed to immobilize the enzyme on the surface of the gas diffusion carbon felt, and a selective uric acid sensor was fabricated by combining immobilized enzyme carbon felt and an oxygen electrode with oxygen gas permeable membrane. This carbon felt has a desirable feature for uricase sensing because an extremely efficient supply of oxygen for the enzymatic reaction can be realized due to its porosity permitting a transfer of oxygen. The current response time required from when sample is added until when current reaches the steady-state value is <5 min. The calibration graph of uric acid showed a linear line in a concentration range from 1x10(-5) to 4x10(-4) M with a detection limit of 4x10(-6) M.
authors.affiliation: Department of Environmental Engineering, Saitama Institute of Technology, 1690 Fusaiji, Okabe, Saitama 369-02, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Uchiyama
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto