title: Determination of rhodamine 123 by sequential injection technique for pharmacokinetic studies in the rat placenta.
abstract: The sequential injection (SIA) technique was applied in pharmacokinetic studies of the transporter-mediated passage of a model substrate, rhodamine 123 (Rho123), through the dually perfused rat term placenta. The method described was used for real-time monitoring of Rho123 concentrations in both the maternal and fetal compartments. Determination was processed by fluorescence detection (lambda(ex)=480 nm, lambda(em)580 nm); calibration curve was linear over the range 0.01-50 mumoll(-1) (r=0.99965), detection limit was 10 nmoll(-1) (3sigma) and RSD2% (10 readings). Transport of Rho123 was scanned under various conditions (ATP-synthesis inhibition) and several inhibitors of P-glycoprotein transporter were tested (e.g. quinidine). The advantages of the modern SIA method-an automated analytical tool providing both fast and precise analysis-were successfully demonstrated for examination of transport profiles to investigate the effect of P-glycoprotein on the placental transfer of Rho123.
authors.affiliation: The Research Centre LN00B125, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic; Department of Analytical Chemistry, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic.
authors.forename: H
authors.initials: H
authors.lastname: Sklenárová
authors.forename: P
authors.initials: P
authors.lastname: Pávek
authors.forename: D
authors.initials: D
authors.lastname: Satínský
authors.forename: P
authors.initials: P
authors.lastname: Solich
authors.forename: R
authors.initials: R
authors.lastname: Karlíček
authors.forename: F
authors.initials: F
authors.lastname: Staud
authors.forename: Z
authors.initials: Z
authors.lastname: Fendrich