title: A unifying nitrososynthase involved in nitrosugar biosynthesis.
abstract: Herein we describe the cloning, functional expression and initial characterization of ORF36 from Micromonospora carbonacae var. africana and rubN8 from Streptomyces achromogenes var. rubradiris. The purified enzymes play the same role, the double-oxidation of TDP-evernosamine to TDP-evernitrosose in the everninomycin and rubradirin pathways, respectively.
authors.affiliation: Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, USA.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Hu
authors.forename: Ahmad
authors.initials: A
authors.lastname: Al-Mestarihi
authors.forename: Catherine L
authors.initials: CL
authors.lastname: Grimes
authors.forename: Daniel
authors.initials: D
authors.lastname: Kahne
authors.forename: Brian O
authors.initials: BO
authors.lastname: Bachmann
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002241:Carbohydrates / Q000096:biosynthesis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D008847:Micromonospora / Q000201:enzymology; D015394:Molecular Structure; D009603:Nitroso Compounds / Q000737:chemistry* / Q000378:metabolism*; D010084:Oxidation-Reduction; D013302:Streptomyces / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D009603:Nitroso Compounds; D000594:Amino Acid Oxidoreductases