title: Isolation and characterisation of a delta5-fatty acid elongase from the marine microalga Pavlova salina.
abstract: The marine microalga Pavlova salina (Haptophyta, Pavlovophyceae) produces lipids containing approximately 50% n-3 long-chain polyunsaturated fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). A full-length cDNA sequence, designated PsElo5, was isolated from P. salina. Sequence alignment showed that the gene was homologous to corresponding ELO-type elongases from other microalgae. Heterologous expression of PsElo5 in yeast and in higher plants confirmed that it encodes a specific Delta5-elongase activity as predicted and, furthermore, within the n-3 pathway, the elongation activity was confined exclusively to EPA.
authors.affiliation: Food Futures National Research Flagship, CSIRO Marine and Atmospheric Research, Hobart, Tasmania, Australia.
authors.forename: Stanley S
authors.initials: SS
authors.lastname: Robert
authors.forename: James R
authors.initials: JR
authors.lastname: Petrie
authors.forename: Xue-Rong
authors.initials: XR
authors.lastname: Zhou
authors.forename: Maged P
authors.initials: MP
authors.lastname: Mansour
authors.forename: Susan I
authors.initials: SI
authors.lastname: Blackburn
authors.forename: Allan G
authors.initials: AG
authors.lastname: Green
authors.forename: Surinder P
authors.initials: SP
authors.lastname: Singh
authors.forename: Peter D
authors.initials: PD
authors.lastname: Nichols
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D056890:Eukaryota / Q000201:enzymology*; D000081031:Fatty Acid Elongases; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis / Q000378:metabolism*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015003:Yeasts
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D005231:Fatty Acids, Unsaturated; C445137:delta5 polyunsaturated fatty acid; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases