CCK-8S inhibited the NMDA-activated current of cultured hippocampal neuron under normal and ethanol exposure conditions.
Whole-cell patch clamp technique was used to study the modulatory effect of Cholecystokin-8S (CCK-8S), a neuropeptide, on NMDA-activated current (I(NMDA)) in cultured rat hippocampal neurons under condition of normal and ethanol exposure. Our study showed that CCK-8S inhibited I(NMDA) in a non-concentration and non-competition dependent manner. Ethanol significantly inhibited I(NMDA), and CCK-8S further inhibited I(NMDA). Our results suggest that CCK-8S is an inhibitory modulator of NMDA receptors under normal and ethanol exposure conditions.
Wei
Xiaofei
X
Department of Physiology, Basic Medical School of Wuhan University, Wuhan 430071, PR China.
Zhang
Zhongshuang
Z
Zhao
Lei
L
Si
Junqiang
J
D000818:Animals; D002478:Cells, Cultured; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D000431:Ethanol / Q000494:pharmacology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D016202:N-Methylaspartate / Q000494:pharmacology; D009433:Neural Inhibition / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D018697:Nootropic Agents / Q000494:pharmacology*; D018408:Patch-Clamp Techniques / Q000379:methods; D051381:Rats; D012844:Sincalide / Q000031:analogs & derivatives* / Q000494:pharmacology
C090019:8-sulfocholecystokinin octapeptide; D002492:Central Nervous System Depressants; D018690:Excitatory Amino Acid Agonists; D018697:Nootropic Agents; D000431:Ethanol; D016202:N-Methylaspartate; D012844:Sincalide