title: Simultaneous absorption of vitamins C and E from topical microemulsions using reconstructed human epidermis as a skin model.
abstract: Antioxidants provide the mainstay for skin protection against free radical damage. The structure of microemulsions (ME), colloidal thermodynamically stable dispersions of water, oil and surfactant, allows the incorporation of both lipophilic (vitamin E) and hydrophilic (vitamin C) antioxidants in the same system. The objective of this work was to investigate the potential of non-thickened (o/w, w/o and gel-like) and thickened (with colloidal silica) ME as carriers for the two vitamins using reconstructed human epidermis (RHE). The amounts of these vitamins accumulated in and permeated across the RHE were determined, together with factors affecting skin deposition and permeation. Notable differences were observed between formulations. The absorption of vitamins C and E in RHE layers was in general enhanced by ME compared to solutions. The incorporation of vitamins in the outer phase of ME resulted in greater absorption than that when vitamins were in the inner phase. The location of the antioxidants in the ME and affinity for the vehicle appear to be crucial in the case of non-thickened ME. Addition of thickener enhanced the deposition of vitamins E and C in the RHE. By varying the composition of ME, RHE absorption of the two vitamins can be significantly modulated.
authors.affiliation: Laboratoire de Recherche et Développement de Pharmacie Galénique Industrielle, Université Lyon I, Lyon, France.
authors.forename: Branka
authors.initials: B
authors.lastname: Rozman
authors.forename: Mirjana
authors.initials: M
authors.lastname: Gasperlin
authors.forename: Estelle
authors.initials: E
authors.lastname: Tinois-Tessoneaud
authors.forename: Fabrice
authors.initials: F
authors.lastname: Pirot
authors.forename: Francoise
authors.initials: F
authors.lastname: Falson
mesh_terms: D000042:Absorption; D000279:Administration, Cutaneous; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry; D001205:Ascorbic Acid / Q000008:administration & dosage*; D003102:Colloids / Q000737:chemistry; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004655:Emulsions / Q000737:chemistry; D004817:Epidermis / Q000187:drug effects*; D005609:Free Radicals; D006801:Humans; D012212:Rheology / Q000379:methods; D012867:Skin / Q000187:drug effects*; D013816:Thermodynamics; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D003102:Colloids; D004337:Drug Carriers; D004655:Emulsions; D005609:Free Radicals; D014810:Vitamin E; D001205:Ascorbic Acid