title: Implementation of terbium-sensitized luminescence in sequential-injection analysis for automatic analysis of orbifloxacin.
abstract: Orbifloxacin (ORBI) is a third-generation fluoroquinolone developed exclusively for use in veterinary medicine, mainly in companion animals. This antimicrobial agent has bactericidal activity against numerous gram-negative and gram-positive bacteria. A few chromatographic methods for its analysis have been described in the scientific literature. Here, coupling of sequential-injection analysis and solid-phase spectroscopy is described in order to develop, for the first time, a terbium-sensitized luminescent optosensor for analysis of ORBI. The cationic resin Sephadex-CM C-25 was used as solid support and measurements were made at 275/545 nm. The system had a linear dynamic range of 10-150 ng mL(-1), with a detection limit of 3.3 ng mL(-1) and an R.S.D. below 3% (n = 10). The analyte was satisfactorily determined in veterinary drugs and dog and horse urine.
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Paraje Las Lagunillas, 23071, Jaén, Spain.
authors.forename: E J
authors.initials: EJ
authors.lastname: Llorent-Martínez
authors.forename: P
authors.initials: P
authors.lastname: Ortega-Barrales
authors.forename: A
authors.initials: A
authors.lastname: Molina-Díaz
authors.forename: A
authors.initials: A
authors.lastname: Ruiz-Medina
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis / Q000652:urine; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000032:analysis / Q000652:urine; D004285:Dogs; D017022:Flow Injection Analysis; D006736:Horses; D008163:Luminescent Measurements / Q000379:methods* / Q000592:standards; D012117:Resins, Synthetic; D013725:Terbium / Q000737:chemistry*; D019155:Veterinary Drugs / Q000032:analysis / Q000652:urine
chemical_list: D000890:Anti-Infective Agents; D012117:Resins, Synthetic; D019155:Veterinary Drugs; D013725:Terbium; D002939:Ciprofloxacin; C095163:orbifloxacin