title: High performance liquid chromatographic determination of N-nitrosoamines by pre-column fluorescence derivatization with acridone-N-acetyl chloride.
abstract: A sensitive high performance liquid chromatographic method for the determination of N-nitrosoamines with pre-column fluorescence derivatization has been developed. N-nitrosoamines are first changed into secondary amines using denitrosation reagent, then react with acridone-N-acetyl chloride (ARC-Cl) to produce corresponding secondary amine derivatives, which exhibit a strong fluorescence. Maximum emission for ARC derivatives is 430 nm (lambda(ex) 404 nm). The labelled derivatives are very stable, less than 4% decomposition occurs after heating at 40 degrees C for 24 h. Fluorescence intensities of derivatives are higher in neutral and alkaline than in acidic solutions. This method, in conjunction with a multi-gradient program, offers a baseline resolution of the ARC derivatives from a linear acetonitrile gradient. Separation is carried out on a reverse phase C(18) column. Derivatization and chromatographic conditions are optimized. The relative standard deviation (n=6) at an analytical concentration of 10 pmol of each N-nitroamine is less than 4.5%. The detection limits at the fmol level. The method described is also suitable for analysis of other amino compounds in different biological samples.
authors.affiliation: Lanzhou Institute of Chemical Physics, Chinese Academy of Science, Lanzhou, 730000, PR China.
authors.forename: J
authors.initials: J
authors.lastname: You
authors.forename: X
authors.initials: X
authors.lastname: Fan
authors.forename: W
authors.initials: W
authors.lastname: Lao
authors.forename: Q
authors.initials: Q
authors.lastname: Ou
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu