Polarography and artificial neural network for the simultaneous determination of nalidixic acid and its main metabolite (7-hydroxymethylnalidixic acid).
Nalidixic acid (NA) and its main metabolite, 7-hydroxymethylnalidixic acid (OH-NA), are simultaneously determined by applying artificial neural networks (ANNs), to their square wave voltammetric signals. The scores of a PCR model, built with the voltammetric data of a set of standard samples, recorded between -0.70 and -1.0V, are used as training set for the net for each compound. The trained nets (ANNs) are used for the simultaneous determination of NA and OH-NA in urine. The recovery values are comprised between 91 and 109% for NA and between 82 and 112% for OH-NA, being these results better than the results obtained by application of partial least squares (PLS) multivariate calibration.
Guiberteau
Agustina
A
Department of Analytical Chemistry, Faculty of Sciences, University of Extremadura, Avda. Elvas s/n, 06071 Badajoz, Spain.
Díaz
Teresa Galeano
TG
Rodríguez Cáceres
María I
MI
Ortiz Burguillos
Juan M
JM
Merás
Isabel Durán
ID
López
Francisco Salinas
FS