title: Establishment of cell lines from mouse embryos with early embryonic lethality.
abstract: It is often difficult to determine molecular mechanisms leading to early embryonic lethality of genetically modified mice due to lack of cells for further analyses. The authors describe here establishment of mouse embryonic fibroblast (MEF) cell lines from gastrulation stage embryos. In this example, using a combination of in vivo and in vitro techniques, the authors successfully generated MEF cell lines that lack both fibronectin (FN) and focal adhesion kinase (FAK).
authors.affiliation: Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA.
authors.forename: Kyle
authors.initials: K
authors.lastname: Howerton
authors.forename: David D
authors.initials: DD
authors.lastname: Schlaepfer
authors.forename: Dusko
authors.initials: D
authors.lastname: Ilic
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology*; D005353:Fibronectins / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D055786:Gene Knockout Techniques; D005804:Genes, Lethal; D051379:Mice
chemical_list: D005353:Fibronectins; D051416:Focal Adhesion Protein-Tyrosine Kinases