title: Determination of urinary oxidative DNA damage marker 8-hydroxy-2'-deoxyguanosine and the association with cigarette smoking.
abstract: 8-hydroxy-2'-deoxyguanosine (8OHdG) has been widely used as a biomarker of oxidative DNA damage in both animal models and human studies. To evaluate the effect of cigarette smoking on oxidative stress, we studied the levels of urinary 8OHdG from smokers and non-smokers and investigated the association with cigarette smoking. The urinary 8OHdG concentrations were determinated by capillary electrophoresis with end-column amprometric detection (CE-AD) after a single-step solid phase extraction (SPE), and then quantitatively expressed as a function of creatinine excretion. To increase the concentration sensitivity, a dynamic pH junction was used and the focusing effect was obvious when using 30mM phosphate (pH 6.50) as sample matrix. The limit of detection is 4.3nM (signal-to-noise ratio S/N=3). The relative standard deviation (R.S.D.) was 1.1% for peak current, and 2.3% for migration time. Based on the selected CE-AD method, it was found that the mean value of urinary 8OHdG levels in the smokers significantly higher than that in non-smokers (31.4+/-18.9 nM versus 14.4+/-7.6 nM , P=0.0004; 23.5+/-21.3 mug g (-)(1) creatinine versus 12.6+/-13.2 mug g (-)(1) creatinine, P=0.028).
authors.affiliation: National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, ZhongShan Road 161, Dalian 116011, PR China.
authors.forename: Qing-Hong
authors.initials: QH
authors.lastname: Yao
authors.forename: Su-Rong
authors.initials: SR
authors.lastname: Mei
authors.forename: Qian-Feng
authors.initials: QF
authors.lastname: Weng
authors.forename: Pu-Duen
authors.initials: PD
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Cai-Ying
authors.initials: CY
authors.lastname: Wu
authors.forename: Guo-Wang
authors.initials: GW
authors.lastname: Xu