Mass transfer effects on DNA hybridization in a flow-through microarray.
The goal of this study is to assess the influence of mass transfer phenomena on DNA hybridization kinetics in a flow-through, porous microarray for fast molecular testing. We present a scaled mathematical model of coupled convection, diffusion and reaction in porous media, which was used to simulate hybridization kinetics and to analyze the influence of convective transport on the reaction rate. In addition to computer simulations, we also present experimental data of hybridization collected on our microarray system for different flow rates. The results reported in this paper provide for a better understanding of the interaction between reaction and mass transfer processes during flow-through hybridization and suggest criteria for system design and optimization.
Mocanu
Daniel
D
Philips Research, High Tech Campus, 5656 AE Eindhoven, The Netherlands. moccad@yahoo.com
Kolesnychenko
Aleksey
A
Aarts
Sonja
S
Troost-Dejong
Amanda
A
Pierik
Anke
A
Vossenaar
Erik
E
Stapert
Henk
H
D000465:Algorithms; D003198:Computer Simulation; D004867:Equipment Design; D007700:Kinetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016062:Porosity; D013997:Time Factors