title: Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to rapidly screen for albuminuria.
abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is used as an alternative method for the rapid diagnosis of albuminuria. This technique requires no further sample pretreatment than simply mixing the urine sample with a MALDI matrix and drying under ambient conditions. The resulting MALDI mass spectra reveal albumin ions having charges ranging from +1 to +5. The detection of albumin is possible using any of the three most common MALDI matrices - sinapinic acid (SA), 2,5-dihydroxybenzoic acid (2,5-DHB), or 4-hydroxy-alpha-cyanocinnamic acid (alpha-CHC). Using this analytical approach, the limit of detection for albumin in urine is 10(-6) M, approximately 5 to 10 times lower than that detectable through conventional chemical testing.
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan. jetea@mail.nsysu.edu.tw
authors.forename: Jentaie
authors.initials: J
authors.lastname: Shiea
authors.forename: Yi-Tzu
authors.initials: YT
authors.lastname: Cho
authors.forename: Yen-Hsiu
authors.initials: YH
authors.lastname: Lin
authors.forename: Chin-Wen
authors.initials: CW
authors.lastname: Chang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Lo
authors.forename: Yi-Chern
authors.initials: YC
authors.lastname: Lee
authors.forename: Hung-Lung
authors.initials: HL
authors.lastname: Ke
authors.forename: Wen-Jeng
authors.initials: WJ
authors.lastname: Wu
authors.forename: Deng-Chyang
authors.initials: DC
authors.lastname: Wu
mesh_terms: D000328:Adult; D000419:Albuminuria / Q000175:diagnosis*; D003373:Coumaric Acids; D005260:Female; D005841:Gentisates; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D055815:Young Adult
chemical_list: D003373:Coumaric Acids; D005841:Gentisates; C007175:alpha-cyano-4-hydroxycinnamate; C073734:sinapinic acid; C010925:2,5-dihydroxybenzoic acid