Low cost combination of DCIP and MCV was better than that of DCIP and OF in the screening for hemoglobin E.
OBJECTIVE
To evaluate hemoglobin E screening tests in a large scale of cases.


METHODS
A cross-sectional descriptive study was conducted Whole blood obtained from subjects was evaluated for CBC, OF, DCIP, and hemoglobin typing.


RESULTS
Five hundred twenty seven hemoglobin E and 280 reference subjects participated. DCIP's sensitivity, specificity, positive predictive value, and negative predictive value were 97.16%, 98.93%, 99.42%, and 95.19%, respectively. These values of OF were 69.12%, 80.00%, 86.67%, and 57.88%, respectively. In the combination of DCIP and OF gave rise to these values of 99.43%, 79.29%, 90.03%, and 96.67%, respectively. Finally the combination of DCIP and MCV < 80 fL resulted in these values to be 99.43%, 98.93%, 99.43%, and 98.93%, respectively. False positive and false negative rate were 1.07% and 0.57%, respectively.


CONCLUSIONS
Combination of DCIP and MCVwas better than that of DCIP and OF in hemoglobin E screening.
Prayongratana
Kannadit
K
Department of Medicine, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand. kannadit_swu@yahoo.com
Polprasert
Chantana
C
Raungrongmorakot
Kasem
K
Tatone
Kanyarat
K
Santiwatanakul
Somchai
S
D015086:2,6-Dichloroindophenol / Q000191:economics*; D000328:Adult; D003430:Cross-Sectional Studies; D004909:Erythrocyte Indices; D005260:Female; D006446:Hemoglobin E / Q000032:analysis*; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000191:economics*; D009996:Osmotic Fragility; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013789:Thalassemia / Q000175:diagnosis* / Q000191:economics
D006446:Hemoglobin E; D015086:2,6-Dichloroindophenol