Hepatitis C virus infection protein network.
A proteome-wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view of the cellular infection. A total of 314 protein-protein interactions between HCV and human proteins was identified by yeast two-hybrid and 170 by literature mining. Integration of this data set into a reconstructed human interactome showed that cellular proteins interacting with HCV are enriched in highly central and interconnected proteins. A global analysis on the basis of functional annotation highlighted the enrichment of cellular pathways targeted by HCV. A network of proteins associated with frequent clinical disorders of chronically infected patients was constructed by connecting the insulin, Jak/STAT and TGFbeta pathways with cellular proteins targeted by HCV. CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins.
de Chassey
B
B
IMAP Team, Inserm Unit 851, Lyon, France.
Navratil
V
V
Tafforeau
L
L
Hiet
M S
MS
Aublin-Gex
A
A
Agaugué
S
S
Meiffren
G
G
Pradezynski
F
F
Faria
B F
BF
Chantier
T
T
Le Breton
M
M
Pellet
J
J
Davoust
N
N
Mangeot
P E
PE
Chaboud
A
A
Penin
F
F
Jacob
Y
Y
Vidalain
P O
PO
Vidal
M
M
André
P
P
Rabourdin-Combe
C
C
Lotteau
V
V
D016174:Hepacivirus / Q000378:metabolism / Q000502:physiology; D006526:Hepatitis C / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
D014764:Viral Proteins