title: Identification of TRAK1 (Trafficking protein, kinesin-binding 1) as MGb2-Ag: a novel cancer biomarker.
abstract: The present study aimed to describe the characterization of an antibody MGb2 that reacts with an epitope on gastric cancer cells, and identification of MGb2 antigen (MGb2-Ag). Immunostaining revealed its distribution in human tissues and demonstrated that the positive rate of MGb2-Ag was 81.48% in gastric cancer, 100% in gastric signet-ring cell carcinoma and mucinous adenocarcinoma, 13.16% in precancerous conditions, and 0% in chronic superficial gastritis. Using Western blotting, immunoprecipitation and MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight mass spectrometry), MGb2-Ag was identified as TRAK1 (Trafficking protein, kinesin-binding 1), a new molecular gained limited recognition. Both MGb2 and commercial anti-TRAK1 Ab recognized prokaryotic expressed TRAK1. Immunostaining characteristics of TRAK1 were identical with MGb2-Ag in continuous sections of paraffin-embedded tissues of gastric tissues. This is the first report that TRAK1/MGb2-Ag is a promising diagnostic marker for gastric cancer and may help to detect signet-ring cell carcinoma and mucinous adenocarcinoma.
authors.affiliation: State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Faming
authors.initials: F
authors.lastname: Zhang
authors.forename: Gui
authors.initials: G
authors.lastname: Ren
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Lu
authors.forename: Bin
authors.initials: B
authors.lastname: Jin
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Xiong
authors.initials: X
authors.lastname: Chen
authors.forename: Zhenxiong
authors.initials: Z
authors.lastname: Liu
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Nie
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D000906:Antibodies / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008297:Male; D008875:Middle Aged; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D000906:Antibodies; D014408:Biomarkers, Tumor; C531552:TRAK1 protein, human