title: Sample-pretreatment procedure for routine liquid chromatographic assay of serum cortisol.
abstract: A specific method for measuring concentrations of cortisol in serum, by preliminary isolation on a "minicolumn" followed by elution and determination by liquid chromatography, is described. This assay requires only 500 microl of serum. The limit of determination of cortisol was found to be 5 microg l . The analytical recovery of cortisol added to serum ranged from 98 to 102%. The coefficients of variation ranged from 2.4 to 3.4% (within-day) and from 3.6 to 8.8% (day-to-day), depending on the cortisol concentration. The method compares well with a commonly used radioimmunological method.
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Roma, Roma, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Laganà
authors.forename: M
authors.initials: M
authors.lastname: Rotatori
authors.forename: G
authors.initials: G
authors.lastname: Vinci
authors.forename: M P
authors.initials: MP
authors.lastname: Colombini
authors.forename: R
authors.initials: R
authors.lastname: Curini