[Influence of neutrophils from severe burn patients on morphology and function of vascular endothelial cells and the protective effect of intervention with Shenmai concoction].
OBJECTIVE
To study the influence of neutrophils from severe burn patient on the morphology and function of vascular endothelial cells (VEC), and the protective effect of Shenmai concoction on VEC.


METHODS
Neutrophils were isolated from the venous blood of healthy volunteers and burn patients [at 6, 24, 48 post burn hour (PBH)], and they were cocultured with VECs (ECV304) in vitro. The viability of VECs was assessed by MTT. The contents of endothelin (ET) land nitric oxide (NO) in supernatant were determined by radioimmunoassay, the ratio between ET-1 and NO (ET-1/NO) was also calculated. Blood samples from burn patients were collected at 24 PBH to repeat experiment as above, but 100 L microL isotonic saline or 100 microL Shenmai concoction in concentrations of 0.5, 2.0, 8.0 g/L was added to the cultures respectively. The effect of Shenmai concoction on above indices and morphology of ECV3O4 were also observed.


RESULTS
Compared with neutrophils from healthy volunteers, the activity of VECs cocultured with neutrophils from burn patients at 6 PBH was obviously decreased, the content of ET-1 obviously increased, especially with neutrophils obtained at 24 PBH (P < 0.01). The content of NO in supernatant of VEC co-cultured with neutrophils from burn patients at 6 PBH was increased obviously (48.9 +/- 2.6 micromol/L, P < 0.01). There were no obvious difference in the content of NO under the stimulation of neutrophils between that of healthy volunteers and burn patients blood obtained at 24, 48 PBH (P > 0.05). With different concentrations of Shenmai, the activity of VECs and the content of NO were obviously increased , while the content of ET-1 was significantly decreased. The morphology of VECs after treatment of neutrophils of 24 PBH and 2.0 g/L Shenmai was similar to normal VECs.


CONCLUSIONS
The morphology, activity of VEC, and ET/NO can be affected by neutrophils after severe burn, especially that of 24 PBH in vitro. Shenmai concoction can ameliorate above indices, thus might possibly exert protective effects on VECs after severe burn.
Huang
Jin-hua
JH
Department of Burn and Plastic Surgery, Subei People Hospital of Jiangsu Province, Yangzhou University Medical Academy, Yangzhou 225001, PR China.
Zhao
Feng-jing
FJ
Liu
Yi-feng
YF
Xu
Gang
G
Zhou
Jie
J
D000328:Adult; D000368:Aged; D002056:Burns / Q000097:blood* / Q000188:drug therapy; D016022:Case-Control Studies; D018920:Coculture Techniques; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D019332:Endothelin-1 / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils; D009569:Nitric Oxide / Q000378:metabolism; D008517:Phytotherapy
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination; D009569:Nitric Oxide