title: Genomic analysis and comparison of Bordetella pertussis isolates circulating in low and high vaccine coverage areas.
abstract: The aim of this study was to analyze Bordetella pertussis isolates circulating, between 1991 and 1995, in Niakhar, Senegal area where the pertussis vaccination coverage was low and to compare them with those circulating in France, before and after introduction of generalized pertussis vaccination for toddlers in 1959. We carried out bacteriological analyses, typing, genotyping and DNA microarray analyses. We found that the isolates circulating in Senegal between 1991 and 1995 are part of the same Pulsed Field Gel Electrophoresis Group, express the same antigens and possess the same gene deletions than isolates circulating in France before the introduction of vaccination, but are different from those circulating in 1991-1995. We confirmed the influence of pertussis vaccination on circulating isolates and that isolates vary with the pertussis cycle.
authors.affiliation: Institut Pasteur, Unite de recherche Prevention et Therapie Moleculaires des Maladies Humaines, URA CNRS 3012, 25 rue du Dr Roux 75724 Paris Cedex 15, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Njamkepo
authors.forename: Thomas
authors.initials: T
authors.lastname: Cantinelli
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Guigon
authors.forename: Nicole
authors.initials: N
authors.lastname: Guiso
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D015373:Bacterial Typing Techniques; D001886:Bordetella pertussis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005602:France; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D046228:Microarray Analysis; D010567:Pertussis Vaccine / Q000008:administration & dosage; D012675:Senegal; D017384:Sequence Deletion; D014917:Whooping Cough / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D010567:Pertussis Vaccine