title: Characterization of esterified hyaluronan-gelatin polymer composites suitable for chondrogenic differentiation of mesenchymal stem cells.
abstract: Composite scaffolds of homogeneously mixed esterified hyaluronan (HY) and gelatin (G) were manufactured with variable component compositions (HY100%; HY95%/G5%; HY70%/G30%). The goals of this study were to analyze the produced composite scaffolds using physical and chemical methods, for example, scanning electron microscopy, IR-spectroscopy, water contact angle, protein assay, and tensile testing as well as to assess the effects of adding gelatin to the composite scaffolds on attachment, proliferation, and chondrogenic differentiation of human mesenchymal stem cells. Numbers of attached cells were significantly higher on the composite material compared to pure hyaluronan at different time points of two-dimensional or three-dimensional cell culture (p< 0.02). In composite scaffolds, a significantly greater amount of cartilage-specific extracellular matrix components was deposited after 28 days in culture (glycosaminoglycan: p < 0.001; collagen: p < 0.001) as compared with 100% hyaluronan scaffolds. Additionally, gelatin-containing composite scaffolds displayed stronger promotion of collagen type II expression than pure hyaluronan scaffolds. The mechanism, based on which gelatin influences cell adhesion, was examined. The effect was inhibited by collagenase treatment of the composites or by addition of alpha5beta1-integrin blocking antibodies to the cell suspension. In summary, the results describe the establishment of a class of composite polymer scaffolds, consisting of esterified hyaluronan and gelatin, which are potentially useful for cell-based tissue engineering approaches using mesenchymal stem cells for chondrogenic differentiation.
authors.affiliation: Department of Trauma Surgery, University Hospital of Regensburg, Regensburg, Germany. angelepeter@aol.com
authors.forename: Peter
authors.initials: P
authors.lastname: Angele
authors.forename: Rainer
authors.initials: R
authors.lastname: Müller
authors.forename: Detlef
authors.initials: D
authors.lastname: Schumann
authors.forename: Carsten
authors.initials: C
authors.lastname: Englert
authors.forename: Johannes
authors.initials: J
authors.lastname: Zellner
authors.forename: Brian
authors.initials: B
authors.lastname: Johnstone
authors.forename: Jung
authors.initials: J
authors.lastname: Yoo
authors.forename: Joachim
authors.initials: J
authors.lastname: Hammer
authors.forename: Johann
authors.initials: J
authors.lastname: Fierlbeck
authors.forename: Martin K
authors.initials: MK
authors.lastname: Angele
authors.forename: Michael
authors.initials: M
authors.lastname: Nerlich
authors.forename: Richard
authors.initials: R
authors.lastname: Kujat
mesh_terms: D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D020219:Chondrogenesis; D055119:Elastic Modulus; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005780:Gelatin; D006820:Hyaluronic Acid