Analysis of anthranilic acid by liquid chromatography.
Analytical methods for the assay of anthranilic acid and for determination of the impurities methyl anthranilate, anthranoylanthranilic acid and 3- and 4-aminobenzoic acid are described. A Microbondapak C18 column is used for both the assay and the impurity determination. The assay is based on isocratic development with a mobile phase of 35:65 v v methanol/pH-3 phosphate buffer, with benzoic acid as internal standard. The impurities are separated by gradient elution. The standard deviation of the assay method is about 1% and the limit of detection for the impurities is about 0.01%.
Schmitt
T M
TM
Central Research and Development, BASF Wyandotte Corporation, Wyandotte, MI 48192, USA.
Ziegler
R J
RJ
Muzher
E S
ES
Doyle
R J
RJ
Freers
J L
JL