title: Systematic approaches to using the FOX hunting system to identify useful rice genes.
abstract: Ectopic gene expression, or the gain-of-function approach, has the advantage that once the function of a gene is known the gene can be transferred to many different plants by transformation. We previously reported a method, called FOX hunting, that involves ectopic expression of Arabidopsis full-length cDNAs in Arabidopsis to systematically generate gain-of-function mutants. This technology is most beneficial for generating a heterologous gene resource for analysis of useful plant gene functions. As an initial model we generated more than 23,000 independent Arabidopsis transgenic lines that expressed rice fl-cDNAs (Rice FOX Arabidopsis lines). The short generation time and rapid and efficient transformation frequency of Arabidopsis enabled the functions of the rice genes to be analyzed rapidly. We screened rice FOX Arabidopsis lines for alterations in morphology, photosynthesis, element accumulation, pigment accumulation, hormone profiles, secondary metabolites, pathogen resistance, salt tolerance, UV signaling, high light tolerance, and heat stress tolerance. Some of the mutant phenotypes displayed by rice FOX Arabidopsis lines resulted from the expression of rice genes that had no homologs in Arabidopsis. This result demonstrated that rice fl-cDNAs could be used to introduce new gene functions in Arabidopsis. Furthermore, these findings showed that rice gene function could be analyzed by employing Arabidopsis as a heterologous host. This technology provides a framework for the analysis of plant gene function in a heterologous host and of plant improvement by using heterologous gene resources.
authors.affiliation: RIKEN Plant Science Center, Yokohama, Kanagawa, Japan.
authors.forename: Youichi
authors.initials: Y
authors.lastname: Kondou
authors.forename: Mieko
authors.initials: M
authors.lastname: Higuchi
authors.forename: Shinya
authors.initials: S
authors.lastname: Takahashi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Takanari
authors.initials: T
authors.lastname: Ichikawa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kuroda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshizumi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tsumoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Horii
authors.forename: Mika
authors.initials: M
authors.lastname: Kawashima
authors.forename: Yukako
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kuriyama
authors.forename: Keiko
authors.initials: K
authors.lastname: Matsui
authors.forename: Miyako
authors.initials: M
authors.lastname: Kusano
authors.forename: Doris
authors.initials: D
authors.lastname: Albinsky
authors.forename: Hideki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Makoto
authors.initials: M
authors.lastname: Suzuki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakakibara
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kojima
authors.forename: Kenji
authors.initials: K
authors.lastname: Akiyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kurotani
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Miki
authors.initials: M
authors.lastname: Fujita
authors.forename: Akiko
authors.initials: A
authors.lastname: Enju
authors.forename: Naoki
authors.initials: N
authors.lastname: Yokotani
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Saitou
authors.forename: Kozue
authors.initials: K
authors.lastname: Ashidate
authors.forename: Naka
authors.initials: N
authors.lastname: Fujimoto
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Mori
authors.forename: Rie
authors.initials: R
authors.lastname: Nanba
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Takata
authors.forename: Kuniko
authors.initials: K
authors.lastname: Uno
authors.forename: Shoji
authors.initials: S
authors.lastname: Sugano
authors.forename: Jun
authors.initials: J
authors.lastname: Natsuki
authors.forename: Joseph Gogo
authors.initials: JG
authors.lastname: Dubouzet
authors.forename: Satoru
authors.initials: S
authors.lastname: Maeda
authors.forename: Miki
authors.initials: M
authors.lastname: Ohtake
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.forename: Kenji
authors.initials: K
authors.lastname: Oda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takatsuji
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Hirochika
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D001482:Base Composition; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D017343:Genes, Plant; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant