title: Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures.
abstract: The effect of biotic elicitors on the production of bilobalide and ginkgolides in Ginkgo biloba cell suspension cultures was studied. The treatment of cell cultures with Candida albicans and Staphylococcus aureus as elicitors increased the amounts of bilobalide (BB), ginkgolide A (GA) and ginkgolide B (GB), with slight growth inhibition. The native bacterial elicitor was more effective for secondary metabolite accumulations both in cells and culture medium than autoclaved. However, exposure times of the cells to the elicitors strongly influenced the production of BB, GA and GB. This study suggests that biotic elicitors can regulate the production of BB, GA and GB either directly or indirectly. These results also describe the establishment of optimum conditions that determine the effects of biotic elicitors on secondary metabolism of bilobalides.
authors.affiliation: Division of Forest Research, Gyeongsangnamdo Forest Environment Research Institute, Jinju 660-870, South Korea.
authors.forename: Seung-Mi
authors.initials: SM
authors.lastname: Kang
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Min
authors.forename: Yong-Duck
authors.initials: YD
authors.lastname: Kim
authors.forename: C S
authors.initials: CS
authors.lastname: Karigar
authors.forename: Seon-Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Gwan-Hyo
authors.initials: GH
authors.lastname: Goo
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Choi
mesh_terms: D002176:Candida albicans; D049109:Cell Proliferation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D003517:Cyclopentanes / Q000378:metabolism*; D003627:Data Interpretation, Statistical; D005663:Furans / Q000378:metabolism*; D020441:Ginkgo biloba / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D046934:Ginkgolides / Q000378:metabolism*; D012717:Sesquiterpenes; D013211:Staphylococcus aureus; D013729:Terpenes / Q000378:metabolism; D000094162:Phytoalexins
chemical_list: D017077:Culture Media, Conditioned; D003517:Cyclopentanes; D005663:Furans; D046934:Ginkgolides; D012717:Sesquiterpenes; D013729:Terpenes; C073710:bilobalide; D000094162:Phytoalexins