title: Bioassays for evaluating the water-extractable genotoxic and toxic potential of soils polluted by metal smelters.
abstract: Physicochemical analyses of polluted soils are limited in their ability to determine all hazardous compounds, their bioavailability, and their combined effects on living organisms. Bioassays, on the other hand, can evaluate environmental quality more accurately. This study assesses the genotoxic potential of water extracts from soil polluted with metals (Pb, Cd, and Zn) by the former lead smelter in zerjav, Slovenia using comet assay with Tetrahymena thermophila and human hepatoma cells (HepG2). In addition, the toxicity of soil samples and their extracts was evaluated using Vibrio fischeri and delayed fluorescence of Lemna minor. Chemical analyses of metals using atomic absorption spectrophotometry (AAS) was performed for comparison. Measurements of the total metal concentrations showed that four of five plots near the former lead smelter were highly contaminated with Pb, Cd, and Zn, but the amount of metals in water/soil extracts was low at all the sampling plots. Genotoxicity was demonstrated using T. thermophila for the majority of the extracts, and HepG2 cells for only some of the extracts. Whereas V. fischeri indicated a gradual decrease in soil toxicity with greater distance from the smelter, the toxicity of extracts did not correlate with proximity. Low concentrations of metals in water extracts stimulated L. minor growth. The results indicate that comet assay with T. thermophila and HepG2 cells and the BSPT with V. fischeri are suitable protocols for screening the genotoxic and toxic potential of water/soil extracts by comet assay, whereas chemical analyses of total metal concentrations in soil do not solely suffice for evaluating metal pollution in the environment. Biological assays are thus crucial for risk assessment.
authors.affiliation: Simon Jenko Primary School, Kranj, Ulica 31. divizije 7a, 4000 Kranj, Slovenia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Vidic
authors.forename: Barbara
authors.initials: B
authors.lastname: Lah
authors.forename: Maja
authors.initials: M
authors.lastname: Berden-Zrimec
authors.forename: Romana
authors.initials: R
authors.lastname: Marinsek-Logar
mesh_terms: D048908:Aliivibrio fischeri / Q000187:drug effects; D000818:Animals; D029064:Araceae / Q000187:drug effects; D002104:Cadmium / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity; D002460:Cell Line; D002478:Cells, Cultured; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage; D007220:Industrial Waste / Q000032:analysis*; D007700:Kinetics; D007854:Lead / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity; D008669:Metallurgy; D008670:Metals / Q000032:analysis / Q000633:toxicity*; D009153:Mutagens / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D012987:Soil / Q000032:analysis; D012989:Soil Pollutants / Q000302:isolation & purification / Q000633:toxicity*; D016808:Tetrahymena thermophila / Q000187:drug effects; D018675:Toxicity Tests / Q000379:methods*; D014867:Water / Q000737:chemistry; D015032:Zinc / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity
chemical_list: D007220:Industrial Waste; D008670:Metals; D009153:Mutagens; D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium; D014867:Water; D007854:Lead; D015032:Zinc