Smart hydrogels containing adenylate kinase: translating substrate recognition into macroscopic motion.
Enzyme-based hybrid hydrogels were prepared by covalently incorporating an adenylate kinase mutant, possessing two thiol groups, into HPMA copolymer- or PEG-based hydrogel structures. The nanoscale conformational change of enzyme, triggered by substrate recognition, translated into macroscopic motion of hydrogels.
Yuan
Weiwei
W
Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84112, USA.
Yang
Jiyuan
J
Kopecková
Pavla
P
Kopecek
Jindrich
J
D000263:Adenylate Kinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D020100:Hydrogels / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D014176:Protein Biosynthesis; D013379:Substrate Specificity
D020100:Hydrogels; D000263:Adenylate Kinase