Quantitative analysis of pesticides by capillary column high performance liquid chromatography combined with solid-phase extraction.
High performance liquid chromatography (HPLC) combined with solid-phase extraction was reported on, for simultaneous analysis of pesticides in this work. The separation of 12 pesticides was achieved on a C(18) capillary column with gradient elution. Sub-microlitre injection volume of the samples and a U-shaped 35 nl flow cell were used to improve the separation and detection. In addition, the method used C(18) solid-phase extraction disks to allow a 250-fold enrichment of the pesticides from fortified water and apple samples. The calculated detection limits range was 0.15-0.8 mug/l. Under the optimal extraction conditions, recoveries of 85-107% for most of the pesticides at 1.0-10.0 mug/l level, were obtained.
Liu
W
W
Department of Chemistry, National University of Singapore, Kent Ridge, Singapore 119260, Singapore.
Lee
H K
HK