title: Effect of calcium-enriched high-fat diet on calcium, magnesium and zinc retention in mice.
abstract: The aim of this work was to assess the effects of a high-fat diet enriched in Ca, which accompanies lower body fat deposition, on mineral depots, as well as to assess the potential role of adaptive thermogenesis in mice. Male mice were fed ad libitum a high-fat (43 %) diet with a Ca content of 4 g/kg from calcium carbonate (control group) or 12 g/kg (42 % from milk powder and the rest from calcium carbonate) (Ca group) for 56 d. Body weight, food intake and urine were periodically collected. Tissue samples were collected when the mice were killed and the composition was determined. Expression of uncoupling proteins was determined by Western blotting. Mineral content was measured by flame atomic absorption spectrometry. Lower body weight gain and fat accretion was found in the Ca group. This could not be attributable to lower gross energy intake or to activation of adaptive thermogenesis. Although significant urine mineral loss was found in the Ca group, preservation of mineral depots in bone was observed. Our data support the fact that adding more Ca to the diet, using a combination of calcium carbonate plus milk powder containing among other things higher Zn and Mg, contributes to counteracting obesity and improving lipid metabolism.
authors.affiliation: Department of Biochemistry, Molecular Biology and Physiology, University of Valladolid, Campus de Soria, Soria, Spain. llpegall@bio.uva.es
authors.forename: Lucía
authors.initials: L
authors.lastname: Pérez-Gallardo
authors.forename: Marta
authors.initials: M
authors.lastname: Gómez
authors.forename: Pilar
authors.initials: P
authors.lastname: Parra
authors.forename: Juana
authors.initials: J
authors.lastname: Sánchez
authors.forename: Andreu
authors.initials: A
authors.lastname: Palou
authors.forename: Francisca
authors.initials: F
authors.lastname: Serra
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002136:Calcium, Dietary / Q000008:administration & dosage*; D004041:Dietary Fats / Q000008:administration & dosage*; D019587:Dietary Supplements; D008099:Liver / Q000033:anatomy & histology; D008274:Magnesium / Q000032:analysis / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008903:Minerals / Q000378:metabolism*; D023421:Models, Animal; D022722:Thermogenesis; D014475:Uncoupling Agents / Q000032:analysis; D015430:Weight Gain; D015032:Zinc / Q000032:analysis / Q000378:metabolism
chemical_list: D002136:Calcium, Dietary; D004041:Dietary Fats; D008903:Minerals; D014475:Uncoupling Agents; D008274:Magnesium; D015032:Zinc; D002118:Calcium