title: Bisursodeoxycholate(ethylenediamine)platinum(II): a new autofluorescent compound. Cytotoxic activity and cell cycle analysis in ovarian and hematological cell lines.
abstract: The present paper describes for the first time an intrinsic fluorescent square-planar platinum(II) complex carrying two ursodeoxycholate ligands ([Pt(UDC)2(en)], where UDC(-) = ursodeoxycholate), that emits at room temperature once free in solution. Kinetic studies were carried out in aqueous solution and in the presence of different NaCl concentrations: 4 mM (similar to cytoplasmic concentration) and 150 mM (similar to plasmatic concentration). This novel compound was synthesized from a [PtCl2(en)] complex and shows increased cytotoxic activity against both resting and cycling HeLa cells, with no toxicity for cell lines derived from neoplastic haematopoietic cells.
authors.affiliation: Centro de Investigación del Cáncer (CIC) Service of Cytometry, Department of Medicine, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Martín
authors.initials: M
authors.lastname: Pérez-Andrés
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Benito
authors.forename: Emilio
authors.initials: E
authors.lastname: Rodríguez-Fernández
authors.forename: Bruna
authors.initials: B
authors.lastname: Corradetti
authors.forename: Daniel
authors.initials: D
authors.lastname: Primo
authors.forename: Juan L
authors.initials: JL
authors.lastname: Manzano
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Criado
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D019337:Hematologic Neoplasms / Q000378:metabolism*; D006801:Humans; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D009944:Organoplatinum Compounds; C542727:bisursodeoxycholate(ethylenediamine)platinum(II)