Comparison of three derivatization reagents for the analysis of Se(IV) based on piazselenol formation and gas chromatography-mass spectrometry.
A comparison between the performance of three derivatizing reagents (4-chloro-o-phenylendiamine, 4-nitro-o-phenylendiamine and 2,3-diaminonaphthalene) for the analysis of selenite in environmental samples using gas chromatography-mass spectrometry (GC-MS) has been evaluated. The effects of different parameters (pH, temperature and time of derivatization, type and volume of organic solvent and time and number of extractions) on the derivatization reaction and extraction of the derivatives have been evaluated. Derivatization using 2,3-diaminonaphthalene took a longer time compared to those obtained using the other reagents. The highest sensitivity was obtained using 4-chloro-o-phenylendiamine. No matrix interferences were observed in tap, river and seawater using the three reagents.
Gómez-Ariza
J L
JL
Departamento de Quimica y Ciencia de los Materiales, Escuela Politécnica Superior, Universidad de Huelva, La Rabida, Huelva, Spain.
Pozas
J A
JA
Giráldez
I
I
Morales
E
E