Determination of l-histidine by modified carbon paste electrode using tetra-3,4-pyridinoporphirazinatocopper(II).
This paper describes a potentiometric method for determination of l-histidine (l-his) in aqueous media, using a carbon paste electrode modified with tetra-3,4-pyridinoporphirazinatocopper(II) (Cu (3,4tppa)). The electrode exhibits linear response to the logarithm of the concentration of l-histidine from 2.4 x 10(-5) to 1.0 x 10(-2)M, with a response slope of -49.5 +/- 1mV and response time of about 1.5min. The detection limit according to IUPAC recommendation was 2.0 x 10(-5)M. The proposed electrode shows a good selectivity for l-his over a wide variety of anions. This chemically modified carbon paste electrode was successfully used for the determination of l-his in a synthetic serum and RANDOX control serum solutions.
Abbaspour
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Chemistry Department, Shiraz University, Shiraz 71454, Iran.
Ghaffarinejad
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Safaei
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