Suppressive effects of triterpenoids on CINC-1 induction in interleukin-1beta-stimulated rat fibroblast NRK-49F cells.
CINC-1 is a member of chemokine family with chemotactic and activating properties to neutrophils. CINC-1 induction in IL-1beta-stimulated rat fibroblast NRK-49F cells was quantitated by a sensitive ELISA. CINC-1 production was increased up to 135 ng/ml from basal 2-6 ng/ml by stimulation with IL-1beta. Steroidal anti-inflammatory drugs including dexamethasone and prednisolone exhibited potent suppressive effects on IL-1beta-induced CINC-1 production. Among 39 kinds of natural triterpenoids tested, acacigenin B exhibited the highest suppressive effects with about 10 muM to be 50% of inhibition on the CINC-1 induction. The suppressive potency of acacigenin B on IL-1beta-induced CINC-1 production was about 10-fold less than that of the steroidal anti-inflammatory drugs.
Ha
J Y
JY
College of Pharmacy, Chungbuk National University, 361-763, Cheongju, Korea.
Min
K R
KR
Kang
S H
SH
Kim
J S
JS
Lee
G I
GI
Kang
S S
SS
Kim
Y
Y