Genome-wide transcriptional responses to sulfite in Saccharomyces cerevisiae.
Sulfite is a commonly used preservative in foods, beverages, and Pharmaceuticals because it is toxic to many microorganisms. In order to understand the global response of Saccharomyces cerevisiae to sulfite, genome-wide transcript profiling following sulfite exposure was obtained. The transcription levels of 21 genes were increased more than 2-fold, while those of 37 genes decreased to a similar extent. Genes involved in carbohydrate metabolism represented the highest proportion of induced genes, which may account for the easily acquired resistance to sulfite. Most of down-regulated genes are involved in transcription, protein biosynthesis, and cell growth. The down-regulation of these genes is thought to reflect growth arrest which occurs during sulfite treatment, allowing cells to save energy. Cells treated with sulfite generated more than 70% of acetaldehyde than untreated cells, suggesting that the increased acetaldehyde production is correlated with the induction of PDC1 gene encoding pyruvate decarboxylase.
Park
Hoon
H
Division of Applied Biological Sciences, Sunmoon University, Asan 336-708, Republic of Korea. hpark@sunmoon.ac.kr
Hwang
Yoon-Sun
YS
D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013447:Sulfites / Q000378:metabolism*; D014158:Transcription, Genetic
D029701:Saccharomyces cerevisiae Proteins; D013447:Sulfites