Novel family of carbohydrate esterases, based on identification of the Hypocrea jecorina acetyl esterase gene.
Plant cell walls have been shown to contain acetyl groups in hemicelluloses and pectin. The gene aes1, encoding the acetyl esterase (Aes1) of Hypocrea jecorina, was identified by amino-terminal sequencing, peptide mass spectrometry, and genomic sequence analyses. The coded polypeptide had 348 amino acid residues with the first 19 serving as a secretion signal peptide. The calculated molecular mass and isoelectric point of the secreted enzyme were 37,088 Da and pH 5.89, respectively. No significant homology was found between the predicated Aes1 and carbohydrate esterases of known families, but putative aes1 orthologs were found in genomes of many fungi and bacteria that produce cell wall-degrading enzymes. The aes1 transcript levels were high when the fungal cells were induced with sophorose, cellulose, oat spelt xylan, lactose, and arabinose. The recombinant Aes1 produced by H. jecorina transformed with aes1 under the cellobiohydrolase I promoter displayed properties similar to those reported for the native enzyme. The enzyme hydrolyzed acetate ester bond specifically. Using 4-nitrophenyl acetate as substrate, the activity of the recombinant enzyme was enhanced by D-xylose, D-glucose, cellobiose, D-galactose, and xylooligosaccharides but not by arabinose, mannose, or lactose. With the use of 4-nitrophenyl-beta-D-xylopyranoside monoacetate as substrate in a beta-xylosidase-coupled assay, Aes1 hydrolyzed positions 3 and 4 with the same efficiency while the H. jecorina acetylxylan esterase 1 exclusively deacetylated the position 2 acetyl group. Aes1 was capable of transacetylating methylxyloside in aqueous solution. The data presented demonstrate that Aes1 and other homologous microbial proteins may represent a new family of esterases for lignocellulose biodegradation.
Li
Xin-Liang
XL
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, United States Department of Agriculture-Agricultural Research Service, 1815 N. University Street, Peoria, Illinois 61604, USA. shinli88@hotmail.com
Skory
Christopher D
CD
Cotta
Michael A
MA
Puchart
Vladimir
V
Biely
Peter
P
D000115:Acetylesterase / Q000737:chemistry / Q000235:genetics*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D020536:Enzyme Activators / Q000494:pharmacology; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D035901:Hypocrea / Q000201:enzymology* / Q000235:genetics; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D021382:Protein Sorting Signals; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D004271:DNA, Fungal; D020536:Enzyme Activators; D005656:Fungal Proteins; D021382:Protein Sorting Signals; D000115:Acetylesterase