title: Micellar electrokinetic capillary chromatography analysis of diuretics in pharmaceutical formulations.
abstract: This paper describes the development of an electrophoretic method used to analyse diuretics, and its use in the analysis of pharmaceutical formulations. Chlorthalidone, furosemide, hydrochlorothiazide and triamterene were separated in 5 min by micellar electrokinetic capillary chromatography using a carrier containing sodium dodecylsulphate as surfactant, and were subsequently detected spectrophotometrically using a diode-array detector. The limits of detection (S/N=3) were in all cases less than 1.2 mugml(-1) for all compounds. Satisfactory repeatability was obtained for migration times (<1.6%) and corrected peak areas (<5.3%) in the analysis of pharmaceutical formulations. The reproducibility was less than 4% for all samples tested. The average recoveries obtained, which ranged between 97 and 100%, testify to the accuracy of the proposed method.
authors.affiliation: Departamento de Quimica Analitica, Nutrición y Bromatologia, Facultad de Quimica, Universidad de La Laguna, E-38204 La Laguna, Tenerife, Spain.
authors.forename: M Luz
authors.initials: ML
authors.lastname: Luis
authors.forename: Sara
authors.initials: S
authors.lastname: Corujedo
authors.forename: Domingo
authors.initials: D
authors.lastname: Blanco
authors.forename: José M G
authors.initials: JM
authors.lastname: Fraga
authors.forename: Ana I
authors.initials: AI
authors.lastname: Jiménez
authors.forename: Francisco
authors.initials: F
authors.lastname: Jiménez
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Arias