Microbacterium kribbense sp. nov., isolated from soil.
A Gram-positive, short, rod-shaped, cream-coloured actinobacterium, designated MSL-04(T), was isolated from a soil sample collected from an agricultural field on Bigeum Island (Republic of Korea) and was subjected to polyphasic characterization to unravel its taxonomic position. The optimum growth temperature and pH were 28-30 degrees C and pH 7.2-7.4. Chemotaxonomic characteristics such as B2beta-type peptidoglycan with glycolyl residues, MK-11 and MK-12 as the major menaquinones and a fatty acid profile containing iso- and anteiso-branched fatty acids placed this organism within the genus Microbacterium. Phylogenetic analysis based on 16S rRNA gene sequences confirmed the position of strain MSL-04(T) in the genus Microbacterium and showed that it belonged to the same clade as Microbacterium ulmi but was distinct from its nearest neighbours. The 16S rRNA gene sequence of strain MSL-04(T) showed the highest levels of similarity with respect to Microbacterium ulmi LMG 20991(T) (97.5 % similarity) and Microbacterium arborescens IFO 3750(T) (97 %). The G+C content of the DNA was 71.0 mol%. The DNA-DNA relatedness between MSL-04(T) and its closest neighbours (<70 %) showed that the strain represents a distinct genomic species. On the basis of the phenotypic and genotypic results, strain MSL-04(T) represents a novel species in the genus Microbacterium, for which the name Microbacterium kribbense sp. nov. is proposed. The type strain is MSL-04(T) (=KCTC 19269(T) =DSM 19265(T)).
Dastager
Syed G
SG
Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
Lee
Jae-Chan
JC
Ju
Yoon-Jung
YJ
Park
Dong-Jin
DJ
Kim
Chang-Jin
CJ
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S