Determination of the binding constant for the inclusion complex between procaine hydrochloride and beta-cyclodextrin by capillary electrophoresis.
The apparent electrophoretic mobilities of procaine hydrochloride (mu(i)) in a series of concentration of beta-cyclodextrin were measured directly by capillary electrophoresis technology. A new mathematical treatment method is proposed, which based on the fact that the molar ratio of the inclusion complex was 1:1 established by spectrophotometry. Using the proposed method, the binding constant of the inclusion complex of procaine hydrochloride with beta-cyclodextrin can be obtained easily. The determination result was in correspondence with those of the spectrophotometric and fluorescence methods.
Li
Nianbing
N
Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, PR China.
Duan
Jianping
J
Chen
Hongqing
H
Chen
Guonan
G