Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of microbial community structure in landfill leachate.
The structures of microbial communities in water samples obtained from a landfill site that had been a source of environmental pollution by emitting hydrogen sulfide were elucidated using polymerase chain reaction-denaturing gradient gel electrophoresis. The microbial communities, which consisted of a limited number of major microorganisms, were stable for several months. Microorganisms capable of degrading such chemical compounds as 2-hydroxybenzothiazole and bisphenol A were observed in landfill leachate. Microorganisms responsible for the production of hydrogen sulfide were not the primary microbes detected, even in water samples obtained from the site of gas emission.
Uchida
Miho
M
Department of Environmental Chemistry and Ecoengineering, Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan. miho@tohtech.ac.jp
Hatayoshi
Haruna
H
Syuku-nobe
Aoi
A
Shimoyama
Takefumi
T
Nakayama
Toru
T
Okuwaki
Akitsugu
A
Nishino
Tokuzo
T
Hemmi
Hisashi
H
D001419:Bacteria / Q000378:metabolism; D001431:Bacteriological Techniques / Q000379:methods*; D001559:Benzhydryl Compounds; D052160:Benzothiazoles / Q000378:metabolism; D004586:Electrophoresis; D004787:Environmental Pollution; D006862:Hydrogen Sulfide; D010636:Phenols / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D052160:Benzothiazoles; D006862:Hydrogen Sulfide; D010636:Phenols; D014874:Water Pollutants, Chemical; C005465:benzothiazole; C006780:bisphenol A; D000098826:Bisphenol A Compounds