Three-dimensional synchronous fluorescence spectrometry for the analysis of three-component alkaloid mixtures.
A study of a simple and sensitive method for the determination of berberine, luguine and sanguinarine in mixtures by normal and synchronous derivative spectrofluorimetry is described. The influence of solvents and other experimental variables is discussed. A three-dimensional plot of both emission-excitation and synchronous spectra obtained by a new software program provides additional information for optimizing instrumental parameters. Linear, normal and derivative calibration graphs are established in the ng/ml range. A statistical analysis of the results and their application to synthetic and natural samples is given.
Sanchez
F G
FG
Department of Analytical Chemistry, Faculty of Sciences, The University, E-29071 Málaga, Spain.
Rubio
A L
AL
Blanco
C C
CC
Suarez
R S
RS