Aqueous two-phase extraction of 2,3-butanediol from fermentation broths by isopropanol/ammonium sulfate system.
A novel aqueous two-phase system consisted of 2-propanol/ammonium sulfate was used for the extraction of 2,3-butanediol from fermentation broths. The maximum partition coefficient and recovery of 2,3-butanediol reached 9.9 and 93.7%, respectively, and more than 99% of the cells and about 85% of the soluble proteins were removed when 34% (w/w) 2-propanol and 20% (w/w) ammonium sulfate were used. The separated cells could be re-used as inocula for subsequent fermentations. The aqueous two-phase system described in this study may have potential application in the extraction of 2,3-butanediol produced by industrial fermentation processes.
Sun
Li-Hui
LH
Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian, 116024, People's Republic of China.
Jiang
Bo
B
Xiu
Zhi-Long
ZL
D019840:2-Propanol / Q000378:metabolism*; D000645:Ammonium Sulfate / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation
D002072:Butylene Glycols; D003470:Culture Media; C026978:2,3-butylene glycol; D019840:2-Propanol; D000645:Ammonium Sulfate