Inhibition of human sperm function and mouse fertilization in vitro by an antibody against cation channel of sperm 1: the contraceptive potential of its transmembrane domains and pore region.
OBJECTIVE
To explore the contraceptive potential of the CatSper1 transmembrane domains and pore region in vitro.


METHODS
In vitro study with human sperm and mouse fertilization.


METHODS
Andrology laboratory of an academic research center.


METHODS
Normozoospermia and viripotent BALB/c mice.


METHODS
The specific binding of an anti-CatSper1 IgG antibody (H-300) to CatSper1 was confirmed by Western blot and immunofluorescence. Sperm from humans and mice were incubated with H-300.


METHODS
The effects of H-300 on human sperm progressive motility, abnormal acrosome, hyperactivated motility, and mouse in vitro fertilization rates were analyzed.


RESULTS
A significant decline in sperm progressive motility was observed after 1, 2, and 4 hours of incubation with H-300; the change was mainly ascribed to the decline of fast progressive motility. Significant inhibition of the hyperactivated motility was observed after 5 hours of incubation with H-300. The incubation of mouse sperm with H-300 before insemination reduced the in vitro fertilization rate to 28% of control levels (72% inhibition).


CONCLUSIONS
CatSper1 may be a potential target for immunocontraception, and the antibody may be a tool to study the function of ion channels in sperm in which relatively fewer methods can be applied.
Li
Honggang
H
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Ding
Xiaofang
X
Centre of Reproductive Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Guan
Huangtao
H
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Xiong
Chengliang
C
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China. Electronic address: clxiongcn@yahoo.com.cn.
D000177:Acrosome / Q000187:drug effects; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000494:pharmacology; D015220:Calcium Channels / Q000276:immunology*; D003272:Contraceptive Agents, Male / Q000276:immunology / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018272:Porins / Q000276:immunology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013081:Sperm Motility / Q000187:drug effects; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
D000888:Antibodies, Anti-Idiotypic; C438823:CATSPER1 protein, human; D015220:Calcium Channels; C488642:Catsper1 protein, mouse; D003272:Contraceptive Agents, Male; D007074:Immunoglobulin G; D018272:Porins