Reactivation of IGFBP7 by DNA demethylation inhibits human colon cancer cell growth in vitro.
BACKGROUND
The insulin-like growth factor binding protein 7 (IGFBP7) gene is regulated by DNA methylation in colon cancer, which was identified in our previous study. In this study, we examined the effects of DNA methylation inhibitor 5-aza-2'-deoxycytidine (5-aza-dC) on IGFBP7 reactivation and cell biological behaviors in vitro to investigate a potential role for 5-aza-dC in treating colorectal cancer.


RESULTS
5-aza-dC treatment showed induction of IGFBP7 transcription in these cancer cells. It consequently led to inhibition of cell growth, cell cycle arrest and apoptosis, suppression of cell migration and invasion in colon cancer cell lines.


METHODS
We examined the effects of 5-aza-dC on reexpression of previously silenced IGFBP7 and its global effects on cell cycle, apoptosis, migration and invasion in three colon cancer cell lines, SW620, HT29 and COLO205.


CONCLUSIONS
Our findings indicate that 5-aza-dC may have anticancer function for colon cancer and restoration of IGFBP7 may involve in the biological effects induced by 5-aza-dC in colon cancer cell lines. These data suggest that 5-aza-dC has clinical potential in the treatment of colorectal cancer.
Lin
Jie
J
Department of Pathology, School of Medicine, Zhejiang University, Hangzhou, China.
Lai
Maode
M
Huang
Qiong
Q
Ruan
Wenjing
W
Ma
Yu
Y
Cui
Jing
J
D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D004247:DNA / Q000235:genetics*; D019175:DNA Methylation; D000077209:Decitabine; D016193:G1 Phase / Q000187:drug effects / Q000235:genetics; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000187:drug effects / Q000235:genetics*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; C102529:insulin-like growth factor binding protein-related protein 1; D000077209:Decitabine; D004247:DNA; D001374:Azacitidine