Effect of gammadelta T cell supernatant on human skin fibroblast proliferation and collagen production--possible role of transforming growth factor-beta and basic fibroblast growth factor.
BACKGROUND
Perivascular mononuclear cell infiltration, vascular injury and diffuse interstitial fibrosis has been shown in systemic sclerosis (SSc). Objective To study a role of cellular immune mechanism inducing fibroblast proliferation and collagen synthesis.


METHODS
The interaction of gammadelta T-cell receptor bearing lymphocyte supernatant to human fibroblast proliferation and collagen synthesis was studied.


RESULTS
The fibroblasts added with gammadelta T cell supernatant showed increased proliferation compared with those without it and that added with alphabeta supernatant. The endothelial cells added with gammadelta T cell supernatants, however, showed significantly decreased proliferation compared with those without it and that added with alphabeta T cell supernatant. The fibroblasts added with endothelial cell supernatant showed increased proliferation compared with those without it. A doubling of collagen synthesis was observed when human skin fibroblasts were maintained in all of the supernatant. The collagen synthesis was inhibited by antitransforming growth factor-beta antibody and antibasic fibroblast growth factor antibody.


CONCLUSIONS
gammadelta T-cell-receptor bearing lymphocyte has proliferative and collagen synthesis activity to human skin fibroblasts, but suppressive to endothelial cells.
Ohtsuka
Tsutomu
T
Department of Dermatology, Dokkyo Medical University, Koshigaya Hospital, Koshigaya, Saitama, Japan. tohtsuka@dokkyomed.ac.jp
D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D042783:Endothelial Cells / Q000166:cytology; D016222:Fibroblast Growth Factor 2 / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000502:physiology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000502:physiology*; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000502:physiology*
D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D003094:Collagen