Determination by hplc of trifluoroacetate levels in plasma and urine of patients anaesthetized with halothane.
A new method is described for determination of trifluoroacetic acid (TFA) in biological fluids. Optimum extraction is achieved by addition of 18-crown-6 ether and acidification of the sample. The 4-bromomethyl-7-methoxycoumarin derivatives of the carboxylic acid are prepared and a sample is subjected to HPLC. A linear analytical curve of peak area against TFA concentrations ranging between 0.2 and 20 mug ml is obtained, and the minimum detectable concentration is estimated to be 0.1 mug ml .
Imbenotte
M
M
Laboratoires de Toxicologie, Hydrologie et Hygiène, Faculté de Pharmacie, Rue du Professeur Laguesse, 59045 Lille, France.
Brice
A
A
Erb
F
F
Haguenoer
J M
JM