title: Analysis of ephedrine in ephedra callus by acetonitrile modified capillary zone electrophoresis.
abstract: A simple method has been developed for the quantitative determination of ephedrine in ephedra callus. The dependence of effective mobility of ephedrine on pH was investigated, and a simulated equation was obtained. The separation was performed in an uncoated capillary and detected at 185 nm. A new Tris-NaOH-H(3)PO(4) run buffer was used and the pH was adjusted to 3.20. To increase the solubility of hydrophobic analytes and improve the separation efficiency, 15% acetonitrile was used in the buffer as a modifier. The content of ephedrine in an ephedra callus sample and an ephedra herba sample were determined with this method, and the result was satisfactory.
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou, People's Republic of China.
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Hu
authors.forename: Z
authors.initials: Z
authors.lastname: Zhao
authors.forename: M
authors.initials: M
authors.lastname: Hooper