Enrichment of benzo[a]pyrene in vegetable oils and determination by HPLC-FL.
We have developed a simple method for the determination of the carcinogen Benzo[a]pyrene (BP) in vegetable oils. The method consists of extraction of the vegetable oil in acetonitrile, concentration to dryness in rotary evaporator and redissolution of the residue in hexane. The purification of the hexane extract was on Sep-Pack Silica Plus cartridges, and the determination of the BP in the isolated extract was by HPLC-FL. Detection and quantification limits were 0.23 and 0.32 mug kg(-1) of olive oil, respectively. Recovery (>93%) and RSD (<4%) were satisfactory. When applied to 18 oil samples, BP levels varied from not detected to 1.99 mug kg(-1).
Vazquez Troche
S
S
Department of Analytical Chemistry, Nutrition and Bromatology, Area of Nutrition and Bromatology, Faculty of Pharmacy, University of Santiago de Compostela, E-15706 Santiago de Compostela, Spain.
García Falcón
M S
MS
González Amigo
S
S
Lage Yusty
M A
MA
Simal Lozano
J
J