title: [Changes of RyR2 mRNA expression in rats suffering from acute myocardial ischemia].
abstract: OBJECTIVE
  To investigate the changes of ryanodine receptor 2 (RyR2) mRNA expression in rats suffering from acute myocardial ischemia.
  
  
  METHODS
  SD rats were divided randomly into normal control group, myocardial ischemia group and sudden death group. The models of myocardial ischemia and sudden cardiac death were induced by intraperitoneal injection of hypophysine. The changes of RyR2 mRNA expression in cardiac sarcoplasmic reticulum (SR) of rats suffering from myocardial ischemia were detected by fluorescent RT-PCR technique.
  
  
  RESULTS
  The levels of RyR2 mRNA in the myocardial ischemia group and sudden death group were significant lower than those in the control group (P<0.05).
  
  
  CONCLUSIONS
  Myocardial ischemia may induce down-regulation of cardiac SR RyR2 mRNA expression.
authors.affiliation: Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Jie
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Zhu
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Fan
mesh_terms: D000818:Animals; D016757:Death, Sudden, Cardiac; D015536:Down-Regulation; D005260:Female; D049429:Forensic Pathology; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel