Mj-DWD, a double WAP domain-containing protein with antiviral relevance in Marsupenaeus japonicus.
The Mj-DWD (Marsupenaeus japonicus' double-WAP domains) gene was originally found up-regulated in virus-resistant shrimp M. japonicus by suppression subtractive hybridization (SSH). The full-length cDNA of Mj-DWD encodes a novel protein containing a KGD (Lys-Gly-Asp) motif and double WAP domains. Performed by quantitative real-time PCR, the expression level of Mj-DWD gene was consistently maintained at a high level in the newly prepared virus-resistant shrimp compared to the normal one. In addition, the Mj-DWD gene was also found to be rapidly up-regulated by WSSV infection during the early phase. Furthermore, the recombinant Mj-DWD, expressed by Pichia pastoris, showed specific protease inhibitory activity on Bacillus subtilis. These findings suggest that Mj-DWD plays an important role in the host defence system against WSSV infection in M. japonicus, possibly through its protease inhibitory activity.
Chen
Dandan
D
School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
He
Nanhai
N
Xu
Xun
X
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004266:DNA Virus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D008894:Milk Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics / Q000276:immunology* / Q000821:virology; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D046848:White spot syndrome virus 1 / Q000276:immunology*
D008894:Milk Proteins; D011994:Recombinant Proteins; C024471:whey acidic proteins