Use of enzymatic catalysis with E.C. 1.2.3.2 xanthine oxidase for the kinetic determination of V(V) at low concentrations.
An enzymatic kinetic method for the determination of V(V) is described, based on the activation of xanthine oxidase-catalysed NADH oxidation, in the presence of xanthine, dithiothreitol and Ag(+) ions. Under these conditions the activating power of V(V) was found to be enhanced. The linear relationship between relative enzyme activity and V(V) concentration allows the enzymatic determination of vanadium in the concentration range 20-500 ng ml , the maximum relative error being +/- 3%, and relative standard deviation less than +/- 2.2%. Possible interferences have been studied.
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Istituto Chimico G. Ciamician dell'Università, Scuola di Specializzazione in Chimica Analitica, Via Selmi 2, 40126 Bologna, Italy.
Stefanelli
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Chiavari
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Tsintiki
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