title: Smart hydrogels containing adenylate kinase: translating substrate recognition into macroscopic motion.
abstract: Enzyme-based hybrid hydrogels were prepared by covalently incorporating an adenylate kinase mutant, possessing two thiol groups, into HPMA copolymer- or PEG-based hydrogel structures. The nanoscale conformational change of enzyme, triggered by substrate recognition, translated into macroscopic motion of hydrogels.
authors.affiliation: Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84112, USA.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Yuan
authors.forename: Jiyuan
authors.initials: J
authors.lastname: Yang
authors.forename: Pavla
authors.initials: P
authors.lastname: Kopecková
authors.forename: Jindrich
authors.initials: J
authors.lastname: Kopecek
mesh_terms: D000263:Adenylate Kinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D020100:Hydrogels / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D014176:Protein Biosynthesis; D013379:Substrate Specificity
chemical_list: D020100:Hydrogels; D000263:Adenylate Kinase