title: N-glycan trimming by glucosidase II is essential for Arabidopsis development.
abstract: Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble heterodimer composed of alpha and beta subunits. Here we present the first characterization of a plant glucosidase II alpha subunit at the molecular level. Expression of the Arabidopsis alpha subunit restored N-glycan maturation capacity in Schizosaccharomyces pombe alpha- or alphabeta-deficient mutants, but with a lower efficiency in the last case. Inactivation of the alpha subunit in a temperature sensitive Arabidopsis mutant blocked N-glycan processing after a first trimming by glucosidase I and strongly affected seedling development.
authors.affiliation: CNRS, UMR 6037, IFRMP 23, Bâtiment Biologie Extension, Faculté des Sciences, Mont-Saint-Aignan, France.
authors.forename: Pravina
authors.initials: P
authors.lastname: Soussilane
authors.forename: Pravina
authors.initials: P
authors.lastname: Soussillane
authors.forename: Cecilia
authors.initials: C
authors.lastname: D'Alessio
authors.forename: Thomas
authors.initials: T
authors.lastname: Paccalet
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Fitchette
authors.forename: Armando J
authors.initials: AJ
authors.lastname: Parodi
authors.forename: Richard
authors.initials: R
authors.lastname: Williamson
authors.forename: Carole
authors.initials: C
authors.lastname: Plasson
authors.forename: Loïc
authors.initials: L
authors.lastname: Faye
authors.forename: Véronique
authors.initials: V
authors.lastname: Gomord
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D036226:Seedlings / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D011134:Polysaccharides; C034251:4-nitrophenyl-alpha-glucosidase; D000520:alpha-Glucosidases