title: Microbial 9alpha-hydroxylase: Epoxidation of 9(11)-dehydro-17alpha-methyl-testosterone.
abstract: Steroid 9alpha-hydroxylase is a key enzyme system in steroid nucleus degradation in company with Delta(1)-dehydrogenase. To examine 9alpha-hydroxylase activity during microbial transformation of steroids, 9(11)-dehydro-17alpha-methyl-testosterone was adopted as a stable substrate for preventing the rupture of steroid nucleus. UsingNocardia restrictus ATCC 14887 capable of introducing a 9alpha-hydroxyl group into steroids, 9alpha, 11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one and 9alpha, 11alpha-oxido-17beta-hydroxy-17alpha-methyl-1, 4-androstadiene-3-one were obtained. These microbiologically transformed products could be used as reference compounds in the enzyme assay.
authors.affiliation: College of Pharmacy, Seoul National University, 151-742, Seoul, Korea.
authors.forename: H K
authors.initials: HK
authors.lastname: Kang
authors.forename: S S
authors.initials: SS
authors.lastname: Lee