title: Analysis of the biological response of endothelial and fibroblast cells cultured on synthetic scaffolds with various hydrophilic/hydrophobic ratios: influence of fibronectin adsorption and conformation.
abstract: In this study we developed polymer scaffolds intended as anchorage rings for cornea prostheses among other applications, and examined their cell compatibility. In particular, a series of interconnected porous polymer scaffolds with pore sizes from 80 to 110 microns were manufactured varying the ratio of hydrophobic to hydrophilic monomeric units along the polymer chains. Further, the effects of fibronectin precoating, a physiological adhesion molecule, were tested. The interactions between the normal human fibroblast cell line MRC-5 and primary human umbilical vein endothelial cells (HUVECs) with the scaffold surfaces were evaluated. Adhesion and growth of the cells was examined by confocal laser scanning microscopy. Whereas MRC-5 fibroblasts showed adhesion and spreading to the scaffolds without any precoating, HUVECs required a fibronectin precoating for adhesion and spreading. Although both cell types attached and spread on scaffold surfaces with a content of up to a 20% hydrophilic monomers, cell adhesion, spreading, and proliferation increased with increasing hydrophobicity of the substrate. This effect is likely due to better adsorption of serum proteins to hydrophobic substrates, which then facilitate cell adhesion. In fact, atomic force microscopy measurements of fibronectin on surfaces representative of our scaffolds revealed that the amount of fibronectin adsorption correlated directly with the hydrophobicity of the surface. Besides cell adhesion we also examined the inflammatory state of HUVECs in contact with the scaffolds. Typical patterns of platelet/endothelial cell adhesion molecule-1 expression were observed at intercellular boarders. HUVECs adhering on the scaffolds retained their proinflammatory response potential as shown by E-selectin mRNA expression after stimulation with lipopolyssacharide (LPS). The proinflammatory activation occurred in most of the cells, thus confirming the presence of a functionally intact endothelium. Little or no expression of the proinflammatory activation markers in the absence of LPS stimulation was observed for HUVECs growing on scaffolds with up to a 20% of hydrophilic component, whereas activation of these markers was observed after stimulation. In conclusion, scaffolds containing up to 20% hydrophilic monomers exhibited excellent cell compatibility toward human fibroblast cell line MRC-5 and human endothelial cells. Atomic force microscopy confirmed that adsorbed serum proteins such as fibronectin probably accounted for the positive correlation of HUVEC adhesion and surface hydrophobicity.
authors.affiliation: Center for Biomaterials, Universidad Politécnica de Valencia, Valencia, Spain. alcamfer@ter.upv.es
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Campillo-Fernández
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Unger
authors.forename: Kirsten
authors.initials: K
authors.lastname: Peters
authors.forename: Sven
authors.initials: S
authors.lastname: Halstenberg
authors.forename: Marina
authors.initials: M
authors.lastname: Santos
authors.forename: Manuel
authors.initials: M
authors.lastname: Salmerón Sánchez
authors.forename: Jose Maria
authors.initials: JM
authors.lastname: Meseguer Dueñas
authors.forename: Manuel
authors.initials: M
authors.lastname: Monleón Pradas
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Gómez Ribelles
authors.forename: C James
authors.initials: CJ
authors.lastname: Kirkpatrick
mesh_terms: D000327:Adsorption; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D019040:E-Selectin / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D005786:Gene Expression Regulation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011108:Polymers / Q000378:metabolism; D011487:Protein Conformation; D013499:Surface Properties; D054457:Tissue Scaffolds; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D019040:E-Selectin; D005353:Fibronectins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011108:Polymers