title: Ribonucleases.
abstract: Ribonucleases (RNases) with different sequence or structural specificities are used for a variety of analytical purposes, including RNA sequencing, mapping, and quantitation. The development of RNase protection assays, structural determination assays, and the production of small interfering RNAs (siRNA) employed in RNA interference (RNAi) experiments has depended on the unique substrate specificities of commercially available RNases, including RNases A, I, T1, V1, HI, III, and Dicer. One very common application for high purity RNase A is also presented in this unit and involves hydrolyzing RNA that contaminates DNA preparations. RNase HII and the placental RNase inhibitor are also discussed.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Nichols
authors.forename: Dongxian
authors.initials: D
authors.lastname: Yue
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D023282:Nuclease Protection Assays / Q000379:methods*; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012260:Ribonucleases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D010940:Plant Proteins; D034741:RNA, Small Interfering; D012313:RNA; D012260:Ribonucleases