A consideration of electrode polishing prior to application of pulsed amperometric detection.
An examination was made of the effect of electrode polishing on the direct pulsed amperometric detection (PAD) and indirect PAD response to penicillin G. The polishing procedure produced a temporary loss of sensitivity for both types of PAD, which over time relaxed back to higher levels. A temporary increase in the electrode kinetics can be observed as well, particularly for a PAD waveform in the crossover region between direct and indirect PAD. In extreme cases, the penicillin G response can undergo a complete polarity reversal. By observing post-polish equilibration times, it was recommended that the direct and indirect PAD user observe 3 and 5 h delay times, respectively, to insure stable electrode response following an electrode polish.
Inoue
A
A
Department of Chemistry, Knox College, Galesburg, IL 61401 USA.
Earley
R L
RL
Lehmann
M W
MW
Welch
L E
LE