Evaluation of a fluorometric-enzymatic method based on 3alpha-hydroxysteroid dehydrogenase for the mycotoxin zearalenone determination in corn.
A new method for the fluorometric determination of zearalenone (ZEN) based on its reaction with betaNADH in the presence of the enzyme 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD) is described. The procedure is based on the change in fluorescence intensity that takes place during the enzymatic reaction (excitation at 340nm and emission at 454nm). The optimum reaction conditions and the analytical characteristics were studied; linear response range (1-10mgl(-1)) and reproducibility (8mgl(-1), 2.7%, n=7). Moreover, a mathematical model explaining the analytical signal is proposed. The method has been applied to zearalenone determination in a spiked corn sample.
Andreu
Yolanda
Y
Analytical Spectroscopy and Sensors Group (GEAS), Department of Analytical Chemistry, Faculty of Science, University of Zaragoza, Plaza San Francisco s/n, E-50009 Zaragoza, Spain.
Castillo
Juan R
JR
de Marcos
Susana
S
Galbán
Javier
J