Placental expression of insulin-like growth factor-I, fibroblast growth factor-basic, and neural cell adhesion molecule in preeclampsia.
OBJECTIVE
To investigate placental expression of insulin-like growth factor-I (IGF-I), fibroblast growth factor-basic (FGF-b), and neural cell adhesion molecule (N-CAM) in preeclampsia.


METHODS
An immunohistochemical analysis using IGF-I, FGF-b, and N-CAM antibodies was conducted on 4% paraformaldehyde-fixed placental tissues of preeclamptic patients (N = 14) and normotensive pregnant subjects (N = 10). Immunostaining patterns of chorionic villi and amniochorionic membranes were assessed.


RESULTS
Significantly increased FGF-b and N-CAM immunoreactivities in cytotrophoblasts and increased FGF-b immunoreactivity in capillary endothelium of chorionic villi of preeclamptic subjects were noted. Significantly increased FGF-b and decreased N-CAM immunoreactivities in extravillous trophoblasts and decidual cells of amniochorionic membranes obtained from preeclamptic subjects were demonstrated. Additionally, a significantly increased IGF-I immunoreactivity was shown in decidual cells of preeclamptic cases.


CONCLUSIONS
Investigation of the regional distribution of IGF-I, FGF-b, and N-CAM at the maternal-fetal interface establishes a better understanding of cell-specific altered growth processes, which may be associated with the pathogenesis of preeclampsia.
Ozkan
Sebiha
S
Department of Obstetrics and Gynecology, School of Medicine, Kocaeli University, Kocaeli, Turkey. sozkan1972@yahoo.com
Vural
Birol
B
Filiz
Serdar
S
Coştur
Pelin
P
Dalçik
Hakki
H
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000209:etiology / Q000378:metabolism*; D011247:Pregnancy; D055815:Young Adult
D019006:Neural Cell Adhesion Molecules; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I