Flow injection spectrophotometric analysis of lead in human saliva for monitoring environmental pollution.
A new highly sensitive, simple and low-cost methodology for the direct determination of Pb (II) with 2-(5-bromo-2-pyridylazo)-5-dimethylaminophenol in ethanolic medium has been developed. The absorption spectroscopy of the complex has been examined in detail, and the chemical variables affecting the sensitivity of procedure studied, optimized and applied to the determination of trace amounts of lead in human saliva. Under the optimal experimental conditions, a precision of 1.61x10(-4) mug cm(-2) was achieved, the molar absorptivity being (epsilon) 5.6x10(4) l mol(-1) cm(-1). An FI technique is proposed, and it is possible to determine trace levels of lead by injection into a steam buffered at pH 7.15, containing 70% ethanol: 30% Tris buffer 3.5x10(-3) mol l(-1) (pH=7.2), 1x10(-4) mol l(-1) 5-BrDMPAP. The FIA configuration allows the analysis of 45 samples per hour. The lower limit of detection (LOD) was 1x10(-7) mol l(-1). The calibration plot was linear at least within two orders of magnitude of lead concentration. The use of an HPLC pump for the FI analysis led to a substantial improvement in the analytical performance of the method, which clearly satisfies the typical requirements for control processes.
Luconi
M
M
Area of Analytical Chemistry, National University of San Luis, Chacabuco y Pedernera, San Luis 5700 CONICET, Argentina.
Silva
M F
MF
Olsina
R A
RA
Fernandez
L
L