The relationship between the extracellular matrix and the angiogenesis and metastasis of laryngeal carcinoma.
OBJECTIVE
We studied the relationships between the growth, metastasis and angiogenesis of laryngeal carcinoma and extracellular matrix protein-1 (ECM1) and hyaluronan (HA).


METHODS
Thirty-three cases of benign and malignant laryngeal tumors were included. Using immunohistochemical staining, we examined the expression of ECM1 and HA in tumors and metastatic lymph nodes and correlated it with counts of microvessel density.


RESULTS
The expression of ECM1 (p = 0.004) and HA (p = 0.036) was significantly different between benign and malignant tumors. The expression of ECM1 was strongly associated with microvessel density (Spearman's rho = 0.513, p = 0.017), with the strongest expression present within caner nests. With respect to HA, expression was significantly associated with more advanced clinical TNM stage (Spearman's rho = 0.521, p = 0.015). The link was stronger in cases where metastasis or recurrence occurred than in those without complication (p = 0.014). No significant relationship was found between ECM1 and HA expression among malignant samples (Spearman's rho = 0.383, p = 0.087).


CONCLUSIONS
ECM1 has a high association with the growth, metastasis and angiogenesis of laryngeal carcinoma. An increased level of HA expression was related to the process of laryngeal carcinogenesis. Specifically, carcinoma cells with high levels of HA secretion had a higher potential for metastasis. No significant relative expression of ECM1 and HA was found.
Han
Zhao
Z
Department of Otorhinolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, PR China.
Lin
Guo-Jing
GJ
Chi
Fang-Lu
FL
Wang
Shu-Yi
SY
Huang
Jian-Min
JM
Liu
Hong-Jian
HJ
Zhang
Lu-Rong
LR
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001707:Biopsy, Needle; D002294:Carcinoma, Squamous Cell / Q000401:mortality / Q000473:pathology / Q000556:secondary*; D015331:Cohort Studies; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000401:mortality / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000473:pathology*; D011336:Probability; D011379:Prognosis; D018570:Risk Assessment; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D016019:Survival Analysis
D014408:Biomarkers, Tumor; C490607:ECM1 protein, human; D016326:Extracellular Matrix Proteins