Determination of milk-oxidised ketone bodies as an acetone derivative by electron capture gas chromatography.
Following the deproteination of the milk sample by means of zinc hexacyanoferrate, acetoacetic acid was decarboxylated by heating; the acetone originally present was then determined together with the acetone formed. The essence of the determination was that acetone reacted with bromine in the presence of sulphuric acid, following which, the bromoacetone formed was extracted into toluene, separated by gas chromatography and measured using an electron capture detector. The calibration curve was found to be linear up to a concentration of 2 mmol l(-1) acetone. The detection limit calculated from the threefold noise level and the slope of the calibration curve was 9x10(-7) mol l(-1). The reliability of the method meets the practical requirements. Its application may be recommended above all for the establishment of subclinical ketosis.
Sarudi
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Department of Chemistry, Faculty of Animal Science, Pannon University of Agriculture, PO Box 16, Guba S.u. 40, 7401 Kaposvár, Hungary.
Visi-Varga
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