title: The use of polyethyleneimine for fabrication of potentiometric cholinesterase biosensors.
abstract: Potentiometric biosensors based on butyrylcholinesterase are developed by co-reticulation of the enzyme with glutaraldehyde on an electropolymerized polyethyleneimine film at the electrode surface. The BuChE-electrode was tested as biochemical sensor for detection of an organophosphorus pesticide, trichlorfon in liquid, the detection being based on the enzyme inhibition. The enzyme electrode showed a detection limit for trichlorfon below 10(-7) M.
authors.affiliation: Laboratoire de Chimie des Matériaux et Interfaces, UFR Sciences and Techniques, 16 route de Gray-La Bouloie, 25030 Besancon Cedex, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Reybier
authors.forename: Sofiane
authors.initials: S
authors.lastname: Zairi
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
authors.forename: Bernard
authors.initials: B
authors.lastname: Fahys