title: Biliary 1-hydroxypyrene as a biomarker of exposure to polycyclic aromatic hydrocarbons in fish.
abstract: OBJECTIVE
  The aim of this study was the assessment of the Svitava and Svratka rivers contamination by polycyclic aromatic hydrocarbons (PAH) using 1-hydroxypyrene (1-OHP) concentrations in fish bile as a biomarker.
  
  
  METHODS
  Levels of 1-OHP were determined by reverse phase HPLC with fluorescence detection. For valid assessment of bile accumulation levels, the 1-OHP concentration was normalized to the biliary protein content. The content of 1-OHP was correlated with the PAH content of river bottom sediments.
  
  
  RESULTS
  The highest level of 1-OHP in fish bile (136.1 ng.mg-1 protein) was found at the locality Rajhradice, which is situated downstream of Brno. Also the greatest level of sum of PAHs was found at this locality (17.1 microg.g-1 dry mass). Significant positive correlation (p< or =0.05) between the level of 1-OHP and sum of PAHs in sediment was found only in case of sediments collected in the same month as fish samples (in June).
  
  
  CONCLUSIONS
  Our results document that 1-OHP in fish bile is a suitable biochemical marker for the assessment of aquatic ecosystem contamination by PAHs.
authors.affiliation: Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. blahovaj@vfu.cz
authors.forename: Jana
authors.initials: J
authors.lastname: Blahova
authors.forename: Kamila
authors.initials: K
authors.lastname: Kruzikova
authors.forename: Klara
authors.initials: K
authors.lastname: Hilscherova
authors.forename: Roman
authors.initials: R
authors.lastname: Grabic
authors.forename: Jarmila
authors.initials: J
authors.lastname: Halirova
authors.forename: Jana
authors.initials: J
authors.lastname: Jurcikova
authors.forename: Tomas
authors.initials: T
authors.lastname: Ocelka
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Svobodova
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D003530:Cyprinidae / Q000502:physiology; D018153:Czech Republic; D004784:Environmental Monitoring; D005399:Fishes / Q000378:metabolism*; D005618:Fresh Water; D009153:Mutagens / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D011506:Proteins / Q000378:metabolism; D011721:Pyrenes / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D011506:Proteins; D011721:Pyrenes; D014874:Water Pollutants, Chemical; C033146:1-hydroxypyrene