Preparation and application of functionalized nanoparticles of CdSe capped with 11-mercaptoundecanoic acid as a fluorescence probe.
A new fluorescence probe, CdSe nanocrystal has been prepared and modified with 11-mercaptoundecanoic acid [HS-(CH(2))(10)-COOH]. The functionalized nanoparticles were characterized using transmission electron microscopy (TEM), ultraviolet-visible (UV-vis) spectroscopy and photoluminescence (PL) spectroscopy. The results demonstrate that CdSe is dispersed homogeneously in aqueous solution and well protected from the environmental oxygen. They can be used as a new fluorescence probe for lysozyme, which was simple, rapid and specific. Under the optimum condition, the response is linearly proportional to the amount of lysozyme from 0.20 to 26.0mugml(-1), and the limit of detection is 0.115mugml(-1). The proposed method has been applied to the determination of lysozyme in egg white, with the recovery of 96-105%.
Zhong
Ping
P
School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
Yu
Ying
Y
Wu
Jianzhong
J
Lai
Yan
Y
Chen
Bo
B
Long
Zhaoyang
Z
Liang
Chunsui
C