title: Application of amperometric sol-gel biosensor to flow injection determination of glucose.
abstract: A glucose biosensor with enzyme immobilised by sol-gel technology was constructed and evaluated. The glucose biosensor reported is based on encapsulated GOX within a sol-gel glass, prepared with 3-aminopropyltriethoxy silane, 2-(3,4-epoxycyclohexyl)-ethyltrimetoxy silane and HCl. A flow system incorporating the amperometric biosensor constructed was developed for the determination of glucose in the 1x10(-4)-5x10(-3) moll(-1) range with a precision of 1.5%. The results obtained for the analysis of electrolytic solution for iv administration and human serum samples showed good agreement between the proposed method and the reference procedure, with relative error <5%.
authors.affiliation: CEQUP/Departamento de Quimica-Fisica, Faculdade de Farmácia (UP), Rua Anibal Cunha, 164-4050 Porto, Portugal; Instituto Superior de Ciências de Saúde, Norte, Rua Central da Gandra, 1317-4580 Gandra, Paredes, Portugal.
authors.forename: Cristina M C M
authors.initials: CM
authors.lastname: Couto
authors.forename: Alberto N
authors.initials: AN
authors.lastname: Araújo
authors.forename: M Conceição B S M
authors.initials: MC
authors.lastname: Montenegro
authors.forename: Jarbas
authors.initials: J
authors.lastname: Rohwedder
authors.forename: Ivo
authors.initials: I
authors.lastname: Raimundo
authors.forename: Célio
authors.initials: C
authors.lastname: Pasquini