The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100.
Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [(3)H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C(14), C(18) or C(18:1) fatty acids (Fas).
Panasiewicz
Mirosława
M
Department of Biochemistry, Medical Center of Postgraduate Education, Marymoncka 99, 01-813 Warszawa, Poland.
Domek
Hanna
H
Hoser
Grazyna
G
Fedoryszak
Natalia
N
Kawalec
Maciej
M
Pacuszka
Tadeusz
T
D018957:CD59 Antigens / Q000302:isolation & purification; D002462:Cell Membrane / Q000737:chemistry*; D005677:G(M1) Ganglioside / Q000737:chemistry* / Q000302:isolation & purification; D018922:HL-60 Cells; D006801:Humans; D017830:Octoxynol; D013010:Sonication
D018957:CD59 Antigens; D005677:G(M1) Ganglioside; D017830:Octoxynol