Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: preliminary study.
OBJECTIVE
In cystic fibrosis (CF) children, we investigated the predictive impact of glutathione S-transferases (GST) activity and genotypes P1, M1 and T1, and antioxidant levels on stage-severity of Pseudomonas aeruginosa lung infection.


METHODS
GST activity was determined in whole blood by spectrophotometry, and GST genotypes by multiplex PCR RFLP for 36 CF and 9 control children. Levels of glutathione in erythrocyte and vitamins A, E and C in plasma were measured by HPLC.


RESULTS
No difference in GST activity and no relationship between GST activity and antioxidant levels were observed in CF children as compared to controls. However, GST activity was lower in CF children with severe clinical status and infection, and the frequency of GSTP1 wild type genotype AA, prevalent in uninfected CF children (75%), decreased in infected ones (33%).


CONCLUSIONS
GST activity and genotype could play an important role in modulating P. aeruginosa lung infection in CF patients.
Feuillet-Fieux
Marie-Noëlle
MN
Assistance Publique-Hôpitaux de Paris, Necker-Enfants Malades Hospital, Laboratory of Biochemistry A, 149 rue de Sèvres, 75015 Paris, France. marie-noelle.feuillet@nck.aphp.fr
Nguyen-Khoa
Thao
T
Loriot
Marie-Anne
MA
Kelly
Mairead
M
de Villartay
Philippe
P
Sermet
Isabelle
I
Verrier
Patricia
P
Bonnefont
Jean-Paul
JP
Beaune
Philippe
P
Lenoir
Gérard
G
Lacour
Bernard
B
D000293:Adolescent; D001205:Ascorbic Acid / Q000097:blood; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003550:Cystic Fibrosis / Q000201:enzymology* / Q000382:microbiology*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005838:Genotype; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011552:Pseudomonas Infections / Q000503:physiopathology*; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity*; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood; D055815:Young Adult
D014801:Vitamin A; D014810:Vitamin E; D005982:Glutathione Transferase; D005978:Glutathione; D001205:Ascorbic Acid