title: Protocol for mosquito rearing (A. gambiae).
abstract: This protocol describes mosquito rearing in the insectary. The insectary rooms are maintained at 28 degrees C and approximately 80% humidity, with a 12 hr. day/night cycle. For this procedure, you'll need mosquito cages, 10% sterile sucrose solution, paper towels, beaker, whatman filter paper, glass feeders, human blood and serum, water bath, parafilm, distilled water, clean plastic trays, mosquito food (described below), mosquito net to cover the trays, vacuum, and a collection chamber to collect adults.
authors.affiliation: Malaria Research Institute, Bloomberg School of Public Health, John Hopkins University, USA.
authors.forename: Suchismita
authors.initials: S
authors.lastname: Das
authors.forename: Lindsey
authors.initials: L
authors.lastname: Garver
authors.forename: George
authors.initials: G
authors.lastname: Dimopoulos
mesh_terms: D000822:Animal Husbandry / Q000295:instrumentation / Q000379:methods*; D000818:Animals; D000852:Anopheles / Q000254:growth & development*; D001769:Blood; D006801:Humans; D006813:Humidity; D017440:Photoperiod; D013696:Temperature