title: Flow-through fluorescence immunosensor for atrazine determination.
abstract: A new flow-through fluoroimmunosensor for atrazine determination based on the use of protein A immobilized on controlled pore glass as immunoreactor is reported. The support, placed in the optical path of the flow cell, allows the 'in situ' quantification of atrazine by on-line antigen-antibody binding upon successive injections of both substances. The immunosensor has a detection limit of 2.1 mug l(-1), a sample speed of about 10 samples per hour, and provides high reproducibility both within-day (3.2% for 5 mug l(-1) and 2.2% for 30 mug l(-1)) and between days. The optimum working concentration range was 2.1-50 mug l(-1). Possible interferences of other triazines like simazine, desethylatrazine (DEA) and desisopropylatrazine (DIA) were evaluated. Simazine and DIA were not cross-reactive; however, the cross-reactivity for DEA was CR=7.7%. The proposed immunosensor was successfully applied to the determination of atrazine in drinking water and citrus fruits.
authors.affiliation: Departamento de Quimica Analitica. Facultad de Ciencias Quimicas, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Turiel
authors.forename: P
authors.initials: P
authors.lastname: Fernández
authors.forename: C
authors.initials: C
authors.lastname: Pérez-Conde
authors.forename: A M
authors.initials: AM
authors.lastname: Gutiérrez
authors.forename: C
authors.initials: C
authors.lastname: Cámara