title: Evaluation of a new Staphylococcus aureus latex agglutination kit, Prolex Staph Xtra, against other third-generation kits.
abstract: Staphylococcus aureus, including methicillin-resistant strains, continues to be a common cause of infection/colonisation, which necessitates accurate and prompt diagnosis in the laboratory. Several rapid agglutination tests that aid this function are available, and some have been modified to improve their performance. One such kit, Prolex Staph Xtra, has been released recently. This study aims to compare this kit with other improved kits (i.e., Pastorex Staph-Plus, Staphaurex Plus and Staphytect Plus) and investigate their ability to confirm the identity of 100 strains of S. aureus. Results showed that 50 were resistant to methicillin. Specificity was checked against 30 strains of coagulase-negative staphylococci and 20 Enterococcus species isolates. Of the four kits tested, Prolex Staph Xtra and Pastorex Staph-Plus proved superior in terms of sensitivity and speed.
authors.affiliation: Microbiology Department, Sheffield Teaching Hospitals NHS Foundation Trust, Northern General Hospital, Herries Road, Sheffield S5 7AU. steve.davies@sth.nhs.uk
authors.forename: S
authors.initials: S
authors.lastname: Davies
authors.forename: S M
authors.initials: SM
authors.lastname: McIntyre
authors.forename: A
authors.initials: A
authors.lastname: McKinven
mesh_terms: D000372:Agglutination Tests / Q000379:methods; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification*
chemical_list: D011933:Reagent Kits, Diagnostic