Evaluation of microwave-assisted extraction for the analysis of polychlorinated biphenyls and organochlorine pesticides in fish.
The efficiency of microwave-assisted extraction (MAE) was evaluated for the analysis of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) in fish. An isotope dilution method was used for quantification via analysis of the samples by gas chromatography and mass spectrometry. MAE solvent, temperature, and time were optimized, and observed concentrations were compared. The MAE results were also compared to those of other extraction techniques (Soxhlet extraction, pressurized liquid extraction, saponification, and homogenization). Concentrations of PCBs and OCPs obtained by MAE at 120 degrees C for 10 min were comparable to those by the other techniques. The results suggest that MAE can be used for the analysis of PCBs and OCPs in fish.
Otake
Takamitsu
T
National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, 1-1-1, Umezono, Tsukuba, Ibaraki 305-8563, Japan. t-ootake@aist.go.jp
Aoyagi
Yoshie
Y
Numata
Masahiko
M
Yarita
Takashi
T
D000818:Animals; D002138:Calibration; D005399:Fishes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D007202:Indicators and Reagents; D008872:Microwaves; D010573:Pesticide Residues / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D012997:Solvents
D006843:Hydrocarbons, Chlorinated; D007202:Indicators and Reagents; D010573:Pesticide Residues; D010575:Pesticides; D012997:Solvents; D011078:Polychlorinated Biphenyls