title: Influence of visceral adiposity on ghrelin secretion and expression in rats during fasting.
abstract: Although circulating ghrelin levels correlate inversely with adiposity at baseline, little is known about the effect of percent visceral adipose tissue value (PVATV) on ghrelin expression and secretion in response to fasting. Our study demonstrated that ghrelin increased with 24-h fasting in rats with the lowest PVATV (less than 6%), after 3 days in rats with intermediate PVATV (6-9%) and 5 days in rats with the highest PVATV (greater than 9%). Ghrelin mRNA in the stomach was increased after 3 days in low-PVATV (5.8+/-0.9%) rats but not in high-PVATV (14+/-1.6%) rats. Therefore, both ghrelin secretion and mRNA were delayed in response to fasting in rats with increased visceral fat. In rats matched for PVATV, but with different body weights, the fasting induced similar levels of increased ghrelin while in rats with different PVATV ghrelin secretion was different in response to fasting, even when body weights were matched in two groups. These data suggested that the initial PVATV, not lean mass, was related to the pattern of plasma ghrelin in response to fasting in rats.
authors.affiliation: Ruijin Hospital State Key Laboratory of Medical Genomics, Shanghai Institute of Endocrinology, Center of Molecular Medicine Department of Radiology, Ruijin Hospital, Shanghai Jiaotong University Medical School, Shanghai 200025, China.
authors.forename: Rong-Ying
authors.initials: RY
authors.lastname: Li
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Shao
authors.forename: Zhi-yuan
authors.initials: ZY
authors.lastname: Wu
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Du
authors.forename: Sheng-Xian
authors.initials: SX
authors.lastname: Li
authors.forename: Shuang-xia
authors.initials: SX
authors.lastname: Zhao
authors.forename: Ke-min
authors.initials: KM
authors.lastname: Chen
authors.forename: Ming-Dao
authors.initials: MD
authors.lastname: Chen
authors.forename: Huai-Dong
authors.initials: HD
authors.lastname: Song
mesh_terms: D050154:Adiposity; D000818:Animals; D001823:Body Composition; D001835:Body Weight; D004041:Dietary Fats; D005215:Fasting; D054439:Ghrelin / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D020738:Leptin / Q000097:blood; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000033:anatomy & histology / Q000502:physiology
chemical_list: D004041:Dietary Fats; D054439:Ghrelin; D020738:Leptin; D012333:RNA, Messenger