Differentiation of two ovine Babesia based on the ribosomal DNA internal transcribed spacer (ITS) sequences.
The first and second internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA gene for six Babesia spp. isolated from different geographic origins were characterized. Varying degrees of ITS1 and ITS2 intra- and inter-species sequence polymorphism were found among these isolates. Phylogenetic analysis of the ITS1-5.8S gene-ITS2 region clearly separated the isolates into two clusters. One held an unidentified Babesia sp. transmitted by Hyalomma anatolicum anatolicum. The second held five other isolates, which were considered to be Babesia motasi. Each Babesia species cluster possessed ITS1 and ITS2 of unique size(s) and species specific nucleotide sequences. The results showed that ITS1, ITS2 and the complete ITS1-5.8S-ITS2 region could be used to discriminate these ovine Babesia spp. effectively.
Niu
Qingli
Q
Gansu Provincal Key Laboratory of Veterinary Parasitology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Xujiaping1, Lanzhou, Gansu 730046, People's Republic of China.
Luo
Jianxun
J
Guan
Guiquan
G
Liu
Zhijie
Z
Ma
Miling
M
Liu
Aihong
A
Gao
Jinliang
J
Ren
Qiaoyun
Q
Li
Youquan
Y
Qiu
Jiaxiang
J
Yin
H
H
D000818:Animals; D001403:Babesia / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D002681:China; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D005819:Genetic Markers; D026863:Ixodidae; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D016415:Sequence Alignment; D012756:Sheep
D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers; D012340:RNA, Ribosomal, 5.8S