Production, isolation and biological activity of nargenicin from Nocardia sp. CS682.
Culture broth of an actinomycete isolate, Nocardia sp. CS682 showed specifically higher antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA). Purified substance from the organism, CS-682, which is active against MRSA and Micrococcus leuteus, is a C(28)H(37)NO(8) (M+H(+), observed: 516.83) and identified as an unusual macrolide antibiotic, nargenicin. The chemical structure of CS-682 was identified by FT-IR, (1)H-NMR, (13)C-NMR, and ((1)H-(1)H and (1)H-(13)H) COSY. The anti-MRSA activity of CS-682 was stronger than that of oxacillin, vancomycin, monensin, erythromycin, and spiramycin. Phylogenetic analysis showed that strain CS682 is closely related to Nocardia tenerifensis DSM 44704(T) (98.7% sequence similarity), followed by N. brasiliensis ATCC 19296(T) (98.4% sequence similarity). The ability of Nocardia sp. CS682 to produce nargenicin was unique.
Sohng
Jae Kyung
JK
Department of Pharmaceutical Engineering, Sun-Moon University, Asan, Chungnam 337-708, Korea.
Yamaguchi
Tokutaro
T
Seong
Chi Nam
CN
Baik
Keun Sik
KS
Park
Seong Chan
SC
Lee
Hyo Jeong
HJ
Jang
So Young
SY
Simkhada
Jaya Ram
JR
Yoo
Jin Cheol
JC
D039903:Actinobacteria / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000900:Anti-Bacterial Agents; D001419:Bacteria / Q000187:drug effects; D002627:Chemistry, Physical; D002876:Chromosomes, Bacterial / Q000737:chemistry; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D024881:Drug Resistance, Bacterial; D007783:Lactones / Q000737:chemistry / Q000494:pharmacology; D016106:Methicillin Resistance / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009615:Nocardia / Q000737:chemistry* / Q000145:classification; D012988:Soil Microbiology; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D007783:Lactones; C034624:nargenicin A1