Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation.
OBJECTIVE
The main drawback of ovarian cryopreservation followed by transplantation is that a large proportion of follicles are lost after transplantation. Thus, effects of erythropoietin (EPO) and desialylated EPO administration on the frozen-thawed canine ovarian xenotransplantation were examined.


METHODS
The protective and survival-promoting effects of EPO and desialylated EPO on the follicles of frozen-thawed canine ovaries after transplantation were examined using NOD-SCID mice. Frozen-thawed dog ovarian tissue with 400 U/kg of EPO or asialo EPO was placed into the ovarian bursa.


RESULTS
At 4 weeks after the transplantation, the ovaries were removed and subjected to histological examination. The survival rate of early primary follicles was 15.2% in the EPO group and 157.6% in the asialo EPO group, in contrast to 10.1% in the untreated group.


CONCLUSIONS
These results demonstrate that administration of asialo EPO could be effectively used to enhance the survival of the follicles of transplanted cryopreserved ovaries.
Suzuki
Hiroshi
H
National Research Center of Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, 080-8555, Japan. hisuzuki@obihiro.ac.jp
Ishijima
Takako
T
Maruyama
Saori
S
Yanagimoto Ueta
Yoshiko
Y
Abe
Yasuyuki
Y
Saitoh
Hideki
H
D000818:Animals; D001212:Asialoglycoproteins / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods*; D004285:Dogs; D004921:Erythropoietin / Q000494:pharmacology*; D005260:Female; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D006080:Ovarian Follicle / Q000187:drug effects* / Q000637:transplantation; D013047:Specific Pathogen-Free Organisms; D014183:Transplantation, Heterologous / Q000379:methods*
D001212:Asialoglycoproteins; D004921:Erythropoietin