Implantation of dedifferentiated fat cells ameliorates habu snake venom-induced chronic renal dysfunction in tenascin-C-deficient mice.
OBJECTIVE
We established dedifferentiated fat (DFAT) cells from mature adipocytes that differentiate to multiple lineages and have characteristics similar to those of mesenchymal stem cells. In this study, we evaluated the effect of implantation of DFAT cells on habu snake venom (HSV)-induced renal dysfunction in tenascin-C knockout (TC-KO) mice.


METHODS
Cultured DFAT cells were incubated with PDGF-BB and immunostained with anti-desmin antibody to determine mesenchymal differentiation. HSV was injected, and DFAT cells from GFP mice were implanted in TC-KO mice via the tail vein. Expression of tenascin-C, transforming growth factor-beta(1) (TGF-beta1), and fibronectin mRNAs in the renal cortex were evaluated by RT-PCR analysis.


RESULTS
Cultured DFAT cells showed desmin immunostaining in response to PDGF-BB.HSV injection induced glomerulosclerosis, which was significantly improved by implantation of DFAT cells. Serum BUN increased after HSV injection and was significantly decreased by implantation of DFAT cells. Tenascin-C mRNA was detected in the renal cortex in implanted mice. Expression of TGF-beta1 and fibronectin mRNAs increased in the renal cortex after HSV injection, and was significantly decreased by implantation of DFAT cells.


CONCLUSIONS
DFAT cells may provide a source for cell therapy for severe progressive renal disease.
Nur
Renaguli
R
Division of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, Nihon University, Tokyo, Japan.
Fukuda
Noboru
N
Matsumoto
Taro
T
Medet
Jumabay
J
Kano
Koichiro
K
Yamamoto
Chii
C
Maruyama
Takashi
T
Endo
Morito
M
Matsumoto
Koichi
K
D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000601:surgery*; D017667:Adipocytes / Q000637:transplantation*; D000818:Animals; D054337:Cell Dedifferentiation; D051379:Mice; D018345:Mice, Knockout; D012910:Snake Venoms / Q000506:poisoning*; D019063:Tenascin / Q000172:deficiency
D012910:Snake Venoms; D019063:Tenascin