Colorimetric determination of some penicillins and cephalosporins with 2-nitrophenylhydrazine hydrochloride.
A simple and sensitive calorimetric method for the determination of some penicillins and cephalosporins is presented. The method is based on reaction with 2-nitrophenylhydrazine hydrochloride in presence of dicyclohexylcarbodi-imide and pyridine. The violet colour of the resulting acid hydrazide is measured at the appropriate wavelength. The method has been applied to determination of these antibiotics in bulk and dosage forms, with a coefficient of variation less than 2%.
Korany
M A
MA
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, University of Alexandria, 21521 Alexandria, Egypt.
Abdel-Hay
M H
MH
Bedair
M M
MM
Gazy
A A
AA