Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to rapidly screen for albuminuria.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is used as an alternative method for the rapid diagnosis of albuminuria. This technique requires no further sample pretreatment than simply mixing the urine sample with a MALDI matrix and drying under ambient conditions. The resulting MALDI mass spectra reveal albumin ions having charges ranging from +1 to +5. The detection of albumin is possible using any of the three most common MALDI matrices - sinapinic acid (SA), 2,5-dihydroxybenzoic acid (2,5-DHB), or 4-hydroxy-alpha-cyanocinnamic acid (alpha-CHC). Using this analytical approach, the limit of detection for albumin in urine is 10(-6) M, approximately 5 to 10 times lower than that detectable through conventional chemical testing.
Shiea
Jentaie
J
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan. jetea@mail.nsysu.edu.tw
Cho
Yi-Tzu
YT
Lin
Yen-Hsiu
YH
Chang
Chin-Wen
CW
Lo
Li-Hua
LH
Lee
Yi-Chern
YC
Ke
Hung-Lung
HL
Wu
Wen-Jeng
WJ
Wu
Deng-Chyang
DC
D000328:Adult; D000419:Albuminuria / Q000175:diagnosis*; D003373:Coumaric Acids; D005260:Female; D005841:Gentisates; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D055815:Young Adult
D003373:Coumaric Acids; D005841:Gentisates; C007175:alpha-cyano-4-hydroxycinnamate; C073734:sinapinic acid; C010925:2,5-dihydroxybenzoic acid