Covalent binding of biorecognition groups to solids using poly(hydromethylsiloxane) as linkage.
By activating SiH bonds, poly(hydromethylsiloxane) can be covalently bound in a first step to various metal or polymer surfaces. In a second step, unreacted SiH bonds can be brought to react with organic compounds having adequate functional groups such as double or triple bonds, carbonyl or hydroxyl groups. This scheme is used to bind biorecognition groups to solids. The novel concept is demonstrated by attaching a newly synthesized biotin derivative to Au. It is shown that the immobilized biotin is capable of binding streptavidin.
Zhang
Xuequn
X
Laboratorium für Organische Chemie, ETH Hönggerberg, CH-8093 Zürich, Switzerland; Institute of Polymers, ETH Zentrum, CH-8092 Zürich, Switzerland.
Deckert
Volker
V
Steiger
Beat
B
Hirayama
Martina K N
MK
Suter
Ulrich W
UW
Pretsch
Ernö
E