title: A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on the immune system of marine mammals.
abstract: The cell lines chosen have demonstrated a positive response in terms of cell proliferation and associated modifications in proteins content, evaluated through DNA and proteins synthesis, at environmentally relevant dose of dioxins, brought by a typical environmental PCDD/F mixture. The response is time and species dependent. After completion of the identification of proteins affected by the intoxication, we will identify a set of specific proteins whose expression is correlated to the dioxin dose and submit the cell culture to the treatment with a single chemical as well as with mixtures. We hope that this will allow us to construct and validate a set of protein biomarkers of exposure to pollutants that will show a predictive aspect for unknown chemicals. The quantitative analysis of the set of biomarkers can then be a more specific bioassay and an alternative to physico-chemical or other already established bioanalytical methods for screening purposes.
authors.affiliation: Mass Spectrometry Laboratory, Faculty of Sciences, University of Liege, Allee De La Chimie, B-6C, Sart-Timan, B-4000 Liege, Belgium.
authors.forename: Cécile
authors.initials: C
authors.lastname: Brenez
authors.forename: Pascal
authors.initials: P
authors.lastname: Gerkens
authors.forename: Gabriel
authors.initials: G
authors.lastname: Mazzucchelli
authors.forename: Thierry
authors.initials: T
authors.lastname: Jauniaux
authors.forename: Gauthier
authors.initials: G
authors.lastname: Eppe
authors.forename: Edwin De
authors.initials: ED
authors.lastname: Pauw
authors.forename: Marie-Claire De
authors.initials: MC
authors.lastname: Pauw-Gillet