title: Simultaneous kinetic spectrophotometric determination of spironolactone and canrenone in urine using partial least-squares regression.
abstract: A method is proposed for the simultaneous determination of spironolactone and canrenone in urine based on the different rates at which they react with sulphuric acid to yield a trienone. Kinetic spectrophotometric data are processed by partial least-squares (PLS) regression. The optimum sulphuric acid concentration and temperature are determined from response surfaces, using PLS methodology to relate both variables to the relative square error of prediction (RSEP, the parameter to be minimized). The relative errors made in the quantitation of each diuretic by the proposed method are less than 5% and the overall error, as RSEP, ranges from 1.06 and 1.44%.
authors.affiliation: Departamento de Quimica Analitica, Nutrición y Bromatologia, Facultad de Quimica, Universidad de La Laguna, E-38204, La Laguna, Tenerife, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Martín
authors.forename: A I
authors.initials: AI
authors.lastname: Jiménez
authors.forename: O
authors.initials: O
authors.lastname: Hernández
authors.forename: F
authors.initials: F
authors.lastname: Jiménez
authors.forename: J J
authors.initials: JJ
authors.lastname: Arias