title: Genetic characterization and polymorphisms for parentage testing of the Jeju horse using 20 microsatellite loci.
abstract: Genetic characterization of the Jeju horse (JH) was performed to construct a correct pedigree of the JH family. A total of 111 horses including 79 JH were genotyped using 20 microsatellite loci. The number of alleles varied from 5 to 11 (mean 7.45) in the JH. The observed heterozygosity and expected heterozygosity ranged from 0.293 to 0.891 and from 0.357 to 0.841, respectively. The polymorphic information contents (PIC) ranged from 0.335 to 0.816. AHT4, ASB2, ASB17, ASB23, CA425, HMS2, HMS3, HTG10, LEX3 and VHL20 loci had relatively high PIC values (> 0.7). The total exclusion probability (PE) of the 20 microsatellite loci was 0.9999 in the JH. These results provide basic information for developing an accurate pedigree and will be useful in making decisions regarding conservation of the JH.
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, South Korea.
authors.forename: Sung-Kyoon
authors.initials: SK
authors.lastname: Choi
authors.forename: Chang-Yeon
authors.initials: CY
authors.lastname: Cho
authors.forename: Sung-Heum
authors.initials: SH
authors.lastname: Yeon
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Cho
authors.forename: Gil-Jae
authors.initials: GJ
authors.lastname: Cho
mesh_terms: D000483:Alleles; D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics; D006736:Horses / Q000235:genetics*; D018895:Microsatellite Repeats; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic
chemical_list: D004247:DNA