The effect of iNOS inhibitors and hyperbaric oxygen treatment in a rat model of experimental colitis.
OBJECTIVE
Our aim was to investigate the effectiveness of aminoguanidine (AMG), an inducible nitric oxide synthase inhibitor, and hyperbaric oxygen (HBO) treatment in an experimental colitis model.


METHODS
We induced colitis in rats. In the control group, we applied 2 ml serum physiologic intraperitoneally for 7 days. In the HBO group, 100% oxygen at 2.4 atm pressure was applied for 7 days. In the AMG group, 100 mg/kg AMG was applied intraperitoneally for 7 days. In the HBO + AMG group, HBO and AMG were applied, respectively. At the end of 7 days, rats were sacrificed and the distal 10 cm part of colon was examined macro- and microscopically.


RESULTS
Severity of colitis and NO activities were reduced by AMG, HBO, and HBO + AMG application. There was histologically significant improvement, especially in the HBO + AMG group.


CONCLUSIONS
Both HBO and AMG were significantly effective in preventing weight loss, reducing NO activities, and severity of colitis, when comparing HBO and AMG separately.
Ercin
Cemal Nuri
CN
Division of Internal Medicine, Department of Gastroenterology, Gulhane School of Medicine, Etlik, Ankara, Turkey. cnercin@hotmail.com
Yesilova
Zeki
Z
Korkmaz
Ahmet
A
Ozcan
Ayhan
A
Oktenli
Cagatay
C
Uygun
Ahmet
A
D019342:Acetic Acid; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000628:therapy*; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D005260:Female; D006146:Guanidines / Q000627:therapeutic use*; D006931:Hyperbaric Oxygenation / Q000379:methods*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D016896:Treatment Outcome
D006146:Guanidines; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D019342:Acetic Acid; C004479:pimagedine