title: Differentiation of rat mesenchymal stem cells transplanted into the subretinal space of sodium iodate-injected rats.
abstract: OBJECTIVE
  The differentiation of rat bone marrow mesenchymal stem cells (MSCs) was investigated in a retinal pigment epithelium (RPE) damage model induced by the administration of sodium iodate.
  
  
  METHODS
  Cultured rat MSCs were transfected with enhanced green fluorescent protein and transplanted into the subretinal space of rats injected 4 days earlier with sodium iodate. Immunofluorescence analysis was performed 5 weeks later.
  
  
  RESULTS
  The transduction efficiency was 99.9%. Viable MSCs were detected 5 weeks after transplantation, mainly in the subretinal space. The cells expressed pan-cytokeratin, glial fibrillary acidic protein and rhodopsin.
  
  
  CONCLUSIONS
  Bone marrow MSCs transplanted into the subretinal space of sodium iodate-injected rats have the ability to differentiate into RPE, photoreceptor and glial lineage cells.
authors.affiliation: Department of Ophthalmology, Affiliated Sixth People's Hospital Shanghai Jiaotong University, Shanghai, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Gong
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.forename: Beiwen
authors.initials: B
authors.lastname: Song
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002454:Cell Differentiation; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000235:genetics; D007267:Injections; D007452:Iodates / Q000633:toxicity*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012162:Retinal Degeneration / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000187:drug effects; D014162:Transfection
chemical_list: D007452:Iodates; D049452:Green Fluorescent Proteins; C032285:sodium iodate