title: Methylated DNA immunoprecipitation (MeDIP).
abstract: Methylated DNA immunoprecipitation (MeDIP) is a versatile immunocapturing approach for unbiased detection of methylated DNA. In brief, genomic DNA is randomly sheared by sonication and immunoprecipitated with a monoclonal antibody that specifically recognizes 5-methylcytidine. The resulting enrichment of methylated DNA in the immunoprecipitated fraction can be determined by PCR to assess the methylation state of individual regions. Alternatively, MeDIP can be combined with large-scale analysis using microarrays as a genome-wide experimental readout. This protocol has been applied to generate comprehensive DNA methylation profiles on a genome-wide scale in mammals and plants, and further to identify abnormally methylated genes in cancer cells.
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Fabio
authors.initials: F
authors.lastname: Mohn
authors.forename: Michael
authors.initials: M
authors.lastname: Weber
authors.forename: Dirk
authors.initials: D
authors.lastname: Schübeler
authors.forename: Tim-Christoph
authors.initials: TC
authors.lastname: Roloff
mesh_terms: D000818:Animals; D001483:Base Sequence; D018899:CpG Islands; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D014764:Viral Proteins
chemical_list: D017931:DNA Primers; C440255:Ocr protein, bacteriophage T7; D014764:Viral Proteins; D004247:DNA