Ruthenium polypyridyl peptide conjugates: membrane permeable probes for cellular imaging.
Two novel polyarginine labelled ruthenium polypyridyl dyes are reported, one conjugated to five, (Ru-Ahx-R5), and one to eight arginine residues, (Ru-Ahx-R8); both complexes exhibit long-lived, intense, and oxygen-sensitive luminescence; (Ru-R8) is passively, efficiently and very rapidly transported across the cell membrane into the cytoplasm without requirement for its permeablisation.
Neugebauer
Ute
U
The Biomedical Diagnostics Institute, Dublin City University, Dublin 9, Ireland.
Pellegrin
Yann
Y
Devocelle
Marc
M
Forster
Robert J
RJ
Signac
William
W
Moran
Niamh
N
Keyes
Tia E
TE
D001792:Blood Platelets / Q000166:cytology; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability; D003593:Cytoplasm / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008163:Luminescent Measurements; D015394:Molecular Structure; D009101:Multiple Myeloma / Q000175:diagnosis* / Q000473:pathology; D009842:Oligopeptides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism*; D010100:Oxygen / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014018:Tissue Distribution
D005456:Fluorescent Dyes; D009842:Oligopeptides; D009942:Organometallic Compounds; D011725:Pyridines; D012428:Ruthenium; D010100:Oxygen