[Transfection with human adenovirus type 5 early-region 1A protects the primary human fibroblasts from ras-induced premature senescence in vitro].
OBJECTIVE
To investigate the role of human adenovirus type 5 (Ad5) early-region 1A (E1A) in premature senescence of human fibroblasts induced by Ras activation.


METHODS
Human fibroblasts were cotransduced with E1A, E1A1-143, or their vector (BP) and Ha-RasV12 or its vector (WH). The growth curves and percentages of the apoptotic cells were determined.


RESULTS
Expression of E1A or Ha-RasV12 in human fibroblasts significantly inhibited the cell growth. Transduction of Ha-RasV12 along with E1A or E1A 1-143 into human fibroblast cells resulted in active and rapid cell proliferation.


CONCLUSIONS
E1A can rescue human fibroblasts from Ras-induced premature senescence, and the senescence bypassing activity of E1A resides in its NH2 terminus.
Li
Yi-lei
YL
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lei@fimmu.com
Zheng
Ping
P
Xu
Chong-yuan
CY
Yan
Yuan
Y
D017500:Adenovirus E1A Proteins / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D011905:Genes, ras / Q000502:physiology*; D006801:Humans; D012867:Skin / Q000166:cytology; D014162:Transfection
D017500:Adenovirus E1A Proteins