Magnetic/luminescent core/shell particles synthesized by spray pyrolysis and their application in immunoassays with internal standard.
Many types of fluorescent nanoparticles have been investigated as alternatives to conventional organic dyes in biochemistry; magnetic beads also have a long history of biological applications. In this work we apply flame spray pyrolysis in order to engineer a novel type of nanoparticle that has both luminescent and magnetic properties. The particles have magnetic cores of iron oxide doped with cobalt and neodymium and luminescent shells of europium-doped gadolinium oxide (Eu:Gd(2)O(3)). Measurements by vibrating sample magnetometry showed an overall paramagnetic response of these composite particles. Luminescence spectroscopy showed spectra typical of the Eu ion in a Gd(2)O(3) host-a narrow emission peak centred near 615 nm. Our synthesis method offers a low-cost, high-rate synthesis route that enables a wide range of biological applications of magnetic/luminescent core/shell particles. Using these particles we demonstrate a novel immunoassay format with internal luminescent calibration for more precise measurements.
Dosev
Dosi
D
Department of Mechanical and Aeronautical Engineering, University of California Davis, One Shields Avenue, Davis, CA 95616, USA.
Nichkova
Mikaela
M
Dumas
Randy K
RK
Gee
Shirley J
SJ
Hammock
Bruce D
BD
Liu
Kai
K
Kennedy
Ian M
IM