Microcystin-RR induces apoptosis in fish lymphocytes by generating reactive oxygen species and causing mitochondrial damage.
Microcystin-RR (MCRR), a very widespread toxic microcystin in China, has previously been proved to induce sensitive apoptosis in Carassius auratus lymphocytes in vitro. This study focused on the role of intracellular Ca(2+), mitochondrial membrane potential, reactive oxygen species, and intracellular ATP in the mechanism of MCRR-induced apoptotic toxicity to fish lymphocytes. Compared with controls, administration of MCRR (10 nmol L(-1)) caused a massive calcium influx resulting in elevation of reactive oxygen species (ROS), rapid disruption of mitochondrial membrane potential (DeltaPsim), and depletion of ATP. This study provided a possible mechanism for the cytotoxicity of microcystins to fish lymphocytes.
Zhang
H
H
College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, PR China.
Zhang
J
J
Chen
Y
Y
Zhu
Y
Y
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002273:Carcinogens / Q000494:pharmacology*; D005260:Female; D006054:Goldfish / Q000502:physiology*; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008387:Marine Toxins; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D052998:Microcystins / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors
D002273:Carcinogens; D008387:Marine Toxins; D052998:Microcystins; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; C063855:microcystin RR; D002118:Calcium