A monoclonal IgM directed against immunodominant catalase B of cell wall of Aspergillus fumigatus exerts anti-A. fumigatus activities.
Aspergillus fumigatus, a ubiquitous fungus, has been reported to cause human diseases like allergic pulmonary aspergillosis, aspergilloma and invasive infection. Limited spectrum and emergence of resistance has become a serious problem with available antifungals. Therefore, an alternative approach is required for successful treatment of mycoses. In the present study, immunogenic protein profile of A. fumigatus cell wall was generated using two-dimensional-gel electrophoresis and three hybridomas producing monoclonal antibodies (MAbs; IgM) were selected after fusion experiments. Of these three MAbs, MAb-7 exhibited potent in vitro inhibitory activity, which was confirmed by MTT assay, fluorescence-activated cell sorter analysis and immuno-fluorescence studies, and the protein was identified as catalase B using MALDI-TOF-MS.
Chaturvedi
Ashok K
AK
Division of Fermentation Technology, Medical Mycology Lab, Central Drug Research Institute, Lucknow, India.
Kumar
Rohitashw
R
Kumar
Awanit
A
Shukla
Praveen K
PK
D000818:Animals; D000908:Antibodies, Fungal / Q000302:isolation & purification / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification / Q000494:pharmacology*; D000946:Antigens, Fungal / Q000276:immunology; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000276:immunology; D002374:Catalase / Q000037:antagonists & inhibitors*; D002473:Cell Wall / Q000201:enzymology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005656:Fungal Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology; D007075:Immunoglobulin M / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability / Q000187:drug effects*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D007075:Immunoglobulin M; D002374:Catalase