Assessing accumulation and biological effect of hydrophobic organic contaminants in water using caged Japanese medaka and deployed triolein-embedded cellulose acetate membranes.
Applicability of triolein-embedded cellulose acetate membrane (TECAM) to accumulation and potential biological effect assessment for hydrophobic organic contaminants (HOCs) was investigated compared with Japanese medaka. The results of field exposure showed that medaka and TECAMs accumulated contaminants in a similar pattern with good correlations between concentrations in medaka and TECAMs based on lipid weight for OCPs (r=0.96, p=0.01, n=9) and PAHs (r=0.73, p=0.01, n=13). Meanwhile, 2,3,7,8-TCDD equivalents (TEQs) of TECAM extracts detected by in vitro H4IIE cell bioassay corresponded well to hepatic EROD activities of exposed fish and TEQs of water samples. We concluded that TECAM could be utilized as a surrogate for biomonitoring organisms to assess the bioaccumulation of HOCs and potential biological effect.
Luo
Jianping
J
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, People's Republic of China.
Ma
Mei
M
Zha
Jinmiao
J
Wang
Zijian
Z
D000818:Animals; D001681:Biological Assay; D015415:Biomarkers; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008567:Membranes, Artificial; D009930:Organic Chemicals / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D009990:Oryzias; D015203:Reproducibility of Results; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism / Q000633:toxicity
D015415:Biomarkers; D008567:Membranes, Artificial; D009930:Organic Chemicals; D014874:Water Pollutants, Chemical; D014867:Water; C005062:acetylcellulose; D002482:Cellulose; D019363:Cytochrome P-450 CYP1A1