Optimal immobilization of penicillinase for lon-selective electrode.
Penicillin sensor was prepared by immobilizing penicillinase (Pcase) on H(+)-selective carboxylated poly (vinyl chloride) (PVC-COOH) membrane or cellulose filter membrane. The immobilization techniques are as follows. Pcase was immobilized with GTH on H(+)-selective PVC-COOH membrane or some amount of BSA was dropped on that membrane. Another method to make immobilization is to mix type I Pcase with GTH and drop on a cellulose filter membrane. According to immobilization techniques, there were some differences in response properties of enzyme electrodes, however, all electrodes responded to Pcase-resistant penicillin derivatives. Pcase immobilized on cellulose filter membrane with H(+)-selective PVC membrane eletrode was more stable and more sensitive to penicillinase-resistant penicillin derivatives than any other immobilization techniques.
Hur
M H
MH
College of Pharmacy, Kyungsung University, 110-1, Daeyundong, Namgu, 608-736, Pusan, Korea.
Kang
H J
HJ
Min
H Y
HY
Lee
J Y
JY
Lee
K H
KH
Ahn
M K
MK