Uterine involution and endometrial function in postpartum pony mares.
OBJECTIVE
To determine endometrial regeneration in postpartum mares by analysis of histologic features, apoptosis and cell proliferation markers, lectin binding, cytokines, and progesterone and estrogen receptors in endometrial biopsy specimens.


METHODS
9 postpartum mares.


METHODS
Mares were examined on postpartum days 1, 9, and 16, and uterine biopsy specimens were obtained for histologic examination. Lectin binding was analyzed histochemically, and expressions of Ki-67 antigen (proliferation marker), lysozyme, and caspase 3 (apoptosis marker) were studied immunohistochemically. Gene expressions for cytokines (interleukin-1beta, -6 and -8 and tumor necrosis factor-alpha), cyclooxygenase 2, prostaglandin-E-synthase, and estrogen and progesterone receptors were determined by use of quantitative real-time PCR assay.


RESULTS
On day 1, neutrophils predominated but by day 9 had largely been replaced by lymphocytes and macrophages. High numbers of cells with staining for caspase 3 were found on day 1, but numbers decreased by day 9. In contrast, the number of cells with staining for Kiel 67 antigen increased between days 1 and 9. Lectin binding to the endometrium changed over time. Relative mRNA expressions for cytokines and prostaglandin-E-synthase did not differ among days. Expressions of progesterone and estrogen receptors were minimal on day 1 and increased by day 9.


CONCLUSIONS
Early postpartum endometrial cells underwent apoptosis, but during the second week, postpartum proliferation of cells predominated. Lectin binding reflected changes in endometrial glycocalyx patterns. Increased expression of estrogen receptors allowed the endometrium to respond to estrogen during foal heat, and in subsequent diestrus, the endometrium was able to respond to progesterone.
Jischa
Susanne
S
Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Sciences, 1210 Vienna, Austria.
Walter
Ingrid
I
Nowotny
Norbert
N
Palm
Franziska
F
Budik
Sven
S
Kolodziejek
Jolanta
J
Aurich
Christine
C
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000032:analysis; D049109:Cell Proliferation; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004717:Endometrium / Q000502:physiology*; D005260:Female; D006736:Horses; D007150:Immunohistochemistry / Q000662:veterinary; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D019394:Ki-67 Antigen / Q000032:analysis; D009113:Muramidase / Q000032:analysis; D049590:Postpartum Period / Q000502:physiology*; D000071876:Prostaglandin-E Synthases; D012333:RNA, Messenger / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism; D012038:Regeneration / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D016207:Cytokines; D017931:DNA Primers; D019394:Ki-67 Antigen; D012333:RNA, Messenger; D011987:Receptors, Steroid; D051546:Cyclooxygenase 2; D009113:Muramidase; D053148:Caspase 3; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases