title: Effects of subchronic metribuzin exposure on common carp (Cyprinus carpio).
abstract: OBJECTIVE
  Effects of metribuzin on biochemical and haematological indices, induction of specific biomarkers and impacts on biometric parameters of Cyprinus carpio were investigated for subchronic conditions.
  
  
  METHODS
  Juvenile fish were exposed to 0.175 mg.L-1 or 1.75 mg.L-1 metribuzin for 28 days. Haematological indices were assessed using unified methods of haematological examination in fish. Biochemical indices were determined by biochemical analyzer, hepatic vitellogenin content was estimated by direct sandwich ELISA. Cytochrome P450 concentration and ethoxyresorufin O-deethylase activity were measured in liver spectrophotometrically and spectrofluorimetrically, respectively.
  
  
  RESULTS
  Increased haematocrit and RBC count were found in fish exposed to 1.75 mg.L-1 metribuzin compared to control fish (p<0.05). Other indices contents and biomarkers levels were not significantly changed by either concentration of metribuzin.
  
  
  CONCLUSIONS
  Sublethal metribuzin pollution may have adverse impacts on haematological parameters in common carp.
authors.affiliation: Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Modra
authors.forename: Ivana
authors.initials: I
authors.lastname: Haluzova
authors.forename: Jana
authors.initials: J
authors.lastname: Blahova
authors.forename: Marcela
authors.initials: M
authors.lastname: Havelkova
authors.forename: Kamila
authors.initials: K
authors.lastname: Kruzikova
authors.forename: Premysl
authors.initials: P
authors.lastname: Mikula
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Svobodova
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D002347:Carps / Q000502:physiology*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004906:Erythrocyte Count; D006540:Herbicides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D014227:Triazines / Q000633:toxicity*; D014818:Vitellogenesis / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D006540:Herbicides; D014227:Triazines; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1; C009235:metribuzin