Amperometric biosensor with physically immobilized glucose oxidase on a PVA cryogel membrane.
A new method of physically immobilizing a biomolecule of analytical interest in poly(vinyl alcohol) cryogels was developed to obtain suitable biosensors. An amperometric glucose sensor was constructed using glucose oxidase immobilized on membranes obtained by a freezing-thawing cyclic process. No chemical cross-linking agent was used. Sensor behaviour was evaluated electrochemically with a hydrogen peroxide electrode. The glucose content in standard solutions was determined and linear calibration curves in the 5 x 10(-5)-3 x 10(-3) mol 1(-1) range were obtained. Temperature and pH effects on the electrochemical response were described and kinetic parameters in the immobilized system were evaluated.
Doretti
L
L
Ufficio Sicurezza e Prevenzione del CNR, Corso Stati Uniti 4, 35127 Padova, Italy.
Ferrara
D
D
Gattolin
P
P
Lora
S
S