title: Preparation and evaluation of non-bonded hyperbranched polymer-coated columns for capillary electrophoresis.
abstract: A series of hyperbranched poly(amine-ester)s based on 1,1,1-trimethylolpropane, methyl acrylate and diethanolamine were synthesized and coated on the inner surface of the fused-silica capillaries by physical adsorption. The most effective coating was the seventh generation hyperbranched poly(amine-ester) coating, which reduced the electroosmotic flow (EOF) greatly and suppressed protein adsorption effectively. The high separation efficiencies for basic proteins were obtained and the coating had a good stability.
authors.affiliation: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; Department of Applied Chemistry, Jinan University, Jinan 250022, PR China.
authors.forename: Chong-Qi
authors.initials: CQ
authors.lastname: Shou
authors.forename: Chang-Li
authors.initials: CL
authors.lastname: Zhou
authors.forename: Chun-Bin
authors.initials: CB
authors.lastname: Zhao
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Guan-Bin
authors.initials: GB
authors.lastname: Li
authors.forename: Li-Ren
authors.initials: LR
authors.lastname: Chen