title: Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells.
abstract: The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 x 10(5) cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.
authors.affiliation: Department of Laboratory Medicine, Seoul National University Boramae Hospital, 425 Shindaebang-dong, Dongjak-gu, Seoul, 156-707, South Korea.
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Yoon
authors.forename: Eun Youn
authors.initials: EY
authors.lastname: Roh
authors.forename: Sue
authors.initials: S
authors.lastname: Shin
authors.forename: Nam Hee
authors.initials: NH
authors.lastname: Jung
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Song
authors.forename: Dong Soon
authors.initials: DS
authors.lastname: Lee
authors.forename: Kyou Sup
authors.initials: KS
authors.lastname: Han
authors.forename: Joung Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Byoung Jae
authors.initials: BJ
authors.lastname: Kim
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Jeon
authors.forename: Kang Sup
authors.initials: KS
authors.lastname: Yoon
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D059630:Mesenchymal Stem Cells; D014465:Ultrasonics; D014470:Umbilical Cord / Q000166:cytology*