Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil.
A Gram-positive, alkaliphilic bacterium, designated strain BA288(T), was isolated from sandy soil. Cells were facultatively anaerobic, endospore-forming rods that were motile by means of peritrichous flagella. The strain grew at 15-40 degrees C and pH 7.0-11.0 (optimally at 30 degrees C and pH 9.0-9.5) and at salinities of 0-4 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BA288(T) belonged to the genus Bacillus and that Bacillus herbersteinensis D-1,5a(T), Bacillus humi LMG 22167(T), Bacillus cohnii DSM 6307(T) and Bacillus litoralis SW-211(T) were the closest neighbours (96.2, 96.0, 96.0 and 95.9 % sequence similarity, respectively). The genomic DNA G+C content was 37.9 mol% and the predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0), C(16 : 0) and iso-C(14 : 0). The peptidoglycan type was A1gamma (meso-diaminopimelic acid). Therefore, on the basis of phylogenetic, phenotypic and chemotaxonomic properties, strain BA288(T) represents a novel species of the genus Bacillus, for which the name Bacillus alkalitelluris sp. nov. is proposed. The type strain is BA288(T) (=KCTC 3947(T) =DSM 16976(T)).
Lee
Jae-Chan
JC
Functional Metabolomics Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
Lee
Gye Suk
GS
Park
Dong-Jin
DJ
Kim
Chang-Jin
CJ
D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012822:Silicon Dioxide