[Study on the sorption and isolation of shikimic acid by 717 anion exchange resin].
OBJECTIVE
To establish a convenient, practical and environmental method for extracting and isolating shikimic acid.


METHODS
The content of shikimic acid was measured by RP-ion-pair HPLC, the effects of different pH, temperature, sample concentration and eluate concentration on 717 anion exchange resin were studied.


RESULTS
The yield of shikimic acid was 96.52% on the condition of 22 degrees C, pH > 6.5, 7.5 mg /ml sample concentration and 0.03 mol/L HCl eluting.


CONCLUSIONS
This method is feasible and suitable for the extraction and isolation of shikimic acid.
Ma
Lian-Ju
LJ
Chongqing Medical University, Chongqing 400016, China. malianjucq@126.com
Liu
Xin
X
Zhuo
Yu-Juan
YJ
Yu
Lu-Rong
LR
D000837:Anion Exchange Resins / Q000737:chemistry*; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D011786:Quality Control; D012117:Resins, Synthetic / Q000737:chemistry; D012765:Shikimic Acid / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D013678:Technology, Pharmaceutical / Q000379:methods*
D000837:Anion Exchange Resins; D000998:Antiviral Agents; D012117:Resins, Synthetic; D012765:Shikimic Acid