Determination of amprolium in feed by a liquid chromatography-mass spectrometry method.
As a consequence of the finding of veterinarian drugs in food European Community banned several compounds like coccidiostats as amprolium (APL). This antibiotic has been used as a preventive and clinical anticoccidial drug in chicken. The 2005/187/CE, 2005/925/EC Recommendations ban the use of amprolium as additive in chicken feed. For this reason a rapid and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed to detect amprolium in chicken feed following the European community proposed technique (1999/27/EC) for sample preparation. Cause the validation is required for the analytical methods used in feed official control, this method was validated according to 2004/882/EC Regulation.
Squadrone
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C.Re.A.A. - National Reference Center for the Surveillance and Monitoring of Animal feed, c/o Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e valle D'Aosta, Via Bologna 148, 10154 Torino, Italy. stefania.squadrone@izsto.it
Mauro
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C
Ferro
G L
GL
Amato
G
G
Abete
M C
MC
D000670:Amprolium / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002138:Calibration; D002645:Chickens; D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods; D003049:Coccidiostats / Q000032:analysis*; D017408:Guidelines as Topic; D013058:Mass Spectrometry / Q000191:economics / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D035501:Uncertainty
D003049:Coccidiostats; D000670:Amprolium