title: Peroxidase-catalysed luminol chemiluminescence method for the determination of glutathione.
abstract: A luminol chemiluminescence (CL) method was developed for the determination of glutathione (GSH). GSH was indirectly determined by measuring the amount of hydrogen peroxide formed during the Cu(II)-catalysed oxidation of GSH with oxygen. The amount of hydrogen peroxide formed was continuously measured using the Arthromyces ramosus peroxidase-catalysed luminol CL reaction. The CL intensities at maximum light emission were linearly correlated with the concentration of GSH over the range 7.5 x 10(-7)-3.0 x 10(-5) M. The detection limit for GSH was about 10 times better than that of the spectrophotometric method using Ellman reagent.
authors.affiliation: Faculty of Engineering, Hokkaido University, Kita-13, Nishi-8, Kita-ku, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kamidate
authors.forename: H
authors.initials: H
authors.lastname: Watanabe