title: Optimization for detection of flunarizine by high performance liquid chromatography/electrospray/mass spectrometry.
abstract: This paper describes a newly developed high performance liquid chromatography/electrospray/mass spectrometry (HPLC/ES/MS) method for the determination of flunarizine (FZ) in artificial cerebrospinal fluid. The optimization for the detection of FZ in biological fluid by LC/ES/MS was investigated. The effects of solvent composition, the addition of modifier and flow rate on the detection of FZ by ES/MS were examined. The detection limit of this method ( approximately 0.8 nM) proved to be much better than previously reported methods. Satisfactory accuracy (98.2-106.0%) of this newly developed method was obtained. The application of this method was demonstrated by analyzing FZ in rat microdialysis samples.
authors.affiliation: P.O. Box 86-72, Department of Chemistry, Soochow University, Taipei, Taiwan, ROC.
authors.forename: M R
authors.initials: MR
authors.lastname: Fuh
authors.forename: C J
authors.initials: CJ
authors.lastname: Hsieh
authors.forename: Y L
authors.initials: YL
authors.lastname: Tai