title: The signal vs. concentration relationships in fluorimetry.
abstract: Formulae for the fluorescence signal are derived which take into account some factors hitherto neglected. From these formulae conditions are deduced, for which a linear relationship exists between the concentration of the fluorescent components and the intensity of the fluorescence. In some cases of practical interest the fulfilment of these conditions cause too low a sensitivity. For such cases a method of optimization is outlined, by means of which the conditions are found which lead to the least deviation from linearity at the required sensitivity.
authors.affiliation: Laboratory for Analytical Chemistry, University of Amsterdam, Nieuwe Achtergracht 125, Amsterdam, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: van Slageren
authors.forename: G
authors.initials: G
authors.lastname: den Boef
authors.forename: W E
authors.initials: WE
authors.lastname: van der Linden