title: Spectrofluorimetric determination of levofloxacin in tablets, human urine and serum.
abstract: A spectrofluorimetric method to determine levofloxacin is proposed and applied to determine the substance in tablets and spiked human urine and serum. The fluorimetric method allow the determination of 20-3000 ng ml(-1) of levofloxacin in aqueous solution containing acetic acid-sodium acetate buffer (pH 4) with lambda(exc)=292 and lambda(em)=494 nm, respectively. Micelle enhanced fluorescence improves the sensibility and allows levofloxacin direct measurement in spiked Human serum (5 mug ml(-1)) and urine (420 mug ml(-1)), in 8 mM sodium dodecyl sulphate solutions at pH 5.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, 41012 Seville, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: González
authors.forename: M
authors.initials: M
authors.lastname: Callejón Mochón
authors.forename: F J
authors.initials: FJ
authors.lastname: de la Rosa