Identification of differentially displayed proteins in cerebrospinal fluid of Parkinson's disease patients: a proteomic approach.
BACKGROUND
Clinical proteomics has been widely used to identify differentially displayed proteins in blood and cerebrospinal fluid (CSF) to understand the molecular and cellular events leading to Parkinson's disease (PD). The close connection between CSF and the brain offers reliable and reproducible way to assess the majority of changes in the brain proteome profile directly into CSF throughout the course of neurodegeneration.


METHODS
We identified the differentially displayed proteins in CSF of PD patients as compared with controls using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and mass spectrometry.


RESULTS
Comparative 2-D PAGE electrophoretograms of CSF of PD patients with case controls and/or neurological controls revealed significant differential display of six protein spots. The differentially displayed proteins were identified as serum albumin precursor, serum albumin chain-A, hemoglobin beta fragment, mutant globin, proline rich repeat 14 (PRR 14) and serum transferrin N-terminal lobe. Although the level of hemoglobin beta fragment and mutant globin was attenuated, serum albumin precursor, serum albumin chain-A, PRR 14 and serum transferrin N-terminal lobe were augmented in PD patients as compared with case controls. The level of serum albumin chain-A, PRR 14 and serum transferrin N-terminal lobe was not significantly altered when compared with neurological controls.


CONCLUSIONS
The results obtained thus suggest that differential display of CSF serum albumin precursor, serum albumin chain-A, PRR 14 and serum transferrin N-terminal lobe could be associated with neuronal dysfunction and hemoglobin/globin with the onset/progression of PD in humans.
Sinha
Ashima
A
Indian Institute of Toxicology Research, Lucknow-226 001, India.
Srivastava
Nalini
N
Singh
Seema
S
Singh
Anand Kumar
AK
Bhushan
Shashi
S
Shukla
Rakesh
R
Singh
Mahendra Pratap
MP
D000328:Adult; D016022:Case-Control Studies; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010300:Parkinson Disease / Q000134:cerebrospinal fluid* / Q000473:pathology; D040901:Proteomics; D015203:Reproducibility of Results
D002556:Cerebrospinal Fluid Proteins