Photometric and electrochemical detection of purine and pyrimidine compounds in reversed-phase and ion-exchange high-pressure liquid chromatography.
An investigation of the optimal conditions for the determination of selected purine and pyrimidine compounds has been made by studying the separation efficiency of reversed-phase columns and ion-exchange columns and comparing the sensitivity of photometric and electrochemical detectors. The combination of ion-exchange HPLC with electrochemical detection proved very good for the separation of electroactive compounds, under the experimental conditions studied. Both types of column allow detection at the picomole level. In general the electrochemical detector appears to be the more sensitive of the two types.
Maria Ghe
A
A
Istituto Chimico "G. Ciamician" dell'Università, Scuola di Specializzazione in Chimica Analitica, Via Selmi 2, 40126 Bologna, Italy.
Chiavari
G
G
Evgenidis
J
J