title: Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: preliminary study.
abstract: OBJECTIVE
  In cystic fibrosis (CF) children, we investigated the predictive impact of glutathione S-transferases (GST) activity and genotypes P1, M1 and T1, and antioxidant levels on stage-severity of Pseudomonas aeruginosa lung infection.
  
  
  METHODS
  GST activity was determined in whole blood by spectrophotometry, and GST genotypes by multiplex PCR RFLP for 36 CF and 9 control children. Levels of glutathione in erythrocyte and vitamins A, E and C in plasma were measured by HPLC.
  
  
  RESULTS
  No difference in GST activity and no relationship between GST activity and antioxidant levels were observed in CF children as compared to controls. However, GST activity was lower in CF children with severe clinical status and infection, and the frequency of GSTP1 wild type genotype AA, prevalent in uninfected CF children (75%), decreased in infected ones (33%).
  
  
  CONCLUSIONS
  GST activity and genotype could play an important role in modulating P. aeruginosa lung infection in CF patients.
authors.affiliation: Assistance Publique-Hôpitaux de Paris, Necker-Enfants Malades Hospital, Laboratory of Biochemistry A, 149 rue de Sèvres, 75015 Paris, France. marie-noelle.feuillet@nck.aphp.fr
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Feuillet-Fieux
authors.forename: Thao
authors.initials: T
authors.lastname: Nguyen-Khoa
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Loriot
authors.forename: Mairead
authors.initials: M
authors.lastname: Kelly
authors.forename: Philippe
authors.initials: P
authors.lastname: de Villartay
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sermet
authors.forename: Patricia
authors.initials: P
authors.lastname: Verrier
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Bonnefont
authors.forename: Philippe
authors.initials: P
authors.lastname: Beaune
authors.forename: Gérard
authors.initials: G
authors.lastname: Lenoir
authors.forename: Bernard
authors.initials: B
authors.lastname: Lacour
mesh_terms: D000293:Adolescent; D001205:Ascorbic Acid / Q000097:blood; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003550:Cystic Fibrosis / Q000201:enzymology* / Q000382:microbiology*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005838:Genotype; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011552:Pseudomonas Infections / Q000503:physiopathology*; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity*; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood; D055815:Young Adult
chemical_list: D014801:Vitamin A; D014810:Vitamin E; D005982:Glutathione Transferase; D005978:Glutathione; D001205:Ascorbic Acid