Salting-out surfactant extraction of porphyrins and metalloporphyrin from aqueous non-ionic surfactant solutions.
A number of cloud point temperature-depressing electrolytes have been investigated for the separation of a non-ionic surfactant (Triton X-100) from aqueous solutions and the corresponding extraction of the organic solutes into the smaller volume surfactant-rich phase using the salting-out method. High extraction efficiencies and preconcentration factors were obtained at room temperature for the extraction of several hydrophilic and hydrophobic metal-free porphyrins (uroporphyrin, coproporphyrin, protoporphyrin and hematoporphyrin) and one metalloporphyrin (iron-protoporphyrin) that were dissolved in the aqueous non-ionic surfactant solutions. Possible mechanisms responsible for the efficient extraction of these important biological molecules into the surfactant-rich layer are discussed.
Horvath
W J
WJ
Department of Chemistry, State University of New York at Binghamton, Binghamton, New York 13902-6000, U.S.A.
Huie
C W
CW