title: Determination of n-acetylmuramoyl-l-alanyl-d-isoglutamine in liposomes by HPLC.
abstract: A method using reversed-phase high-pressure liquid chromatography (with spectrometric detection at 218nm) is described for the determination in new pharmaceutical preparations (liposomes) of a new immunostimulating agent (N-acetylmuramoyl-l-alanyl-d-isoglutamine). Separation was achieved with a mu-bondapak column and phosphate buffer (pH 2.5)-methanol mixture (93:7 v/v) as eluent, at a flow-rate of 2 ml min . Sodium acetate was used as an internal standard. The detector response at 218 nm was linear in the range 10-170 mug ml . The method is simple and accurate.
authors.affiliation: Laboratoires de contrôle de qualité, Pharmacie Centrale des Hôpitaux de Paris, 7, rue de Fer à Moulin, 75221 Paris Cedex 05, France.
authors.forename: E
authors.initials: E
authors.lastname: Postaire
authors.forename: M
authors.initials: M
authors.lastname: Hamon
authors.forename: E
authors.initials: E
authors.lastname: Sponton
authors.forename: D
authors.initials: D
authors.lastname: Pradeau