title: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry monitoring of anthocyanins in extracts from Arabidopsis thaliana leaves.
abstract: Anthocyanins are secondary plant metabolites ubiquitous in the plant kingdom. They have different biological activities, so monitoring their content in plant tissue or in feed/food derived from plants may be an important task in different projects from various fields of molecular biology and biotechnology. Profiling of secondary metabolites with high-performance liquid chromatography/mass spectrometry (HPLC/MS) systems is time-consuming, especially when many samples have to be checked within a defined time frame with a reasonable number of repetitions according to the metabolomic standards. Even application of the advanced ultra-performance liquid chromatography (UPLC)/MS or equivalent systems would require a long time for analysis of numerous samples. We demonstrate the applicability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the assessment of level (concentration) of anthocyanins in leaf tissues of four Arabidopsis thaliana ecotypes grown at normal (20 degrees C/16 degrees C day/night) and decreased (4 degrees C) temperature. The quantitative results were obtained for anthocyanins with MALDI-TOF MS using ferulic acid as a matrix. The amounts of anthocyanins in leaves of A. thaliana varied from 0.3-2.5 microg per gram of leaves for ecotypes Col-0 and C24, respectively, and contents of these markedly increased in plants grown in the cold. The applied analytical method exhibited better repeatability of measurements than obtained with an HPLC/ion trap MS system.
authors.affiliation: Institute of Bioorganic Chemistry PAS, Noskowskiego 12/14, 61-704 Poznań, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Marczak
authors.forename: Piotr
authors.initials: P
authors.lastname: Kachlicki
authors.forename: Piotr
authors.initials: P
authors.lastname: Koźniewski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Skirycz
authors.forename: Paweł
authors.initials: P
authors.lastname: Krajewski
authors.forename: Maciej
authors.initials: M
authors.lastname: Stobiecki
mesh_terms: D000872:Anthocyanins / Q000032:analysis* / Q000737:chemistry; D017360:Arabidopsis / Q000737:chemistry*; D003373:Coumaric Acids; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000872:Anthocyanins; D003373:Coumaric Acids; D010936:Plant Extracts; C004999:ferulic acid