Genistein inhibits proliferation and functions of hypertrophic scar fibroblasts.
Hypertrophic scarring is abnormal proliferation of dermal fibroblasts and excessive deposition of extracellular matrix. To date, despite many studies, treatments have not been satisfactory. Genistein, a potent, specific inhibitor of tyrosine protein kinases (TPKs), has been proved to inhibit many kinds of tumour and some fibrotic diseases. The purpose of this study was to investigate the effects of genistein on the proliferation and functions of hypertrophic scar fibroblasts (HSFBs) and the mechanism by which genistein inhibits TPK signal transduction. The first effect was observed by methyl-thiazol-diphenyl-tetrazolium assay and the second by [gamma-(32)p] adenosine triphosphate incorporation assay. The results demonstrated that genistein inhibits the proliferation and function of HSFBs and changes the TPK signal transduction pathway, which can provide an experimental basis for treating HS with genistein.
Cao
Chuan
C
Department of Plastic and Reconstructive Surgery, Southwestern Hospital, Third Military Medical University, Chongqing, China.
Li
Shirong
S
Dai
Xia
X
Chen
Yanqing
Y
Feng
Zhi
Z
Zhao
Yun
Y
Wu
Jun
J
D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D017439:Cicatrix, Hypertrophic / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D019833:Genistein / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D047428:Protein Kinase Inhibitors / Q000627:therapeutic use*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D015398:Signal Transduction / Q000187:drug effects; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects; D055815:Young Adult
D047428:Protein Kinase Inhibitors; D019833:Genistein; D011505:Protein-Tyrosine Kinases