Simultaneous determination of 12 chemical constituents in the traditional Chinese Medicinal Prescription Xiao-Yao-San-Jia-Wei by HPLC coupled with photodiode array detection.
An HPLC-photodiode array (PDA) detection method was established for the simultaneous determination of 12 components in Xiao-Yao-San-Jia-Wei (XYSJW): geniposide, puerarin, paeoniflorin, ferulic acid, liquiritin, hesperidin, naringin, paeonol, daidzein, glycyrrhizic acid, honokiol, and magnolol. These were separated in less than 70 min using a Waters Symmetry Shield RP 18 column with gradient elution using (A) acetonitrile, (B) water, and (C) acetic acid at a flow rate of 1 ml/min, and with a PDA detector. All calibration curves showed good linear regression (r(2)>0.9992) within the test ranges. The method was validated for specificity, accuracy, precision, and limits of detection. The proposed method enables in a single run the simultaneous identification and determination for quality control of 12 multi-structural components of XYSJW forming the basis of its therapeutic effect.
Zhang
Hongmin
H
Henan Eye Institute and Department of Ophthalmology, Henan Provincial People's Hospital, Zhengzhou 450003, PR China.
Chen
Shiwei
S
Qin
Feng
F
Huang
Xi
X
Ren
Ping
P
Gu
Xinqi
X
D000098:Acetophenones / Q000737:chemistry; D001565:Benzoates / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D001952:Bridged-Ring Compounds / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry; D004365:Drugs, Chinese Herbal; D044950:Flavanones / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry; D019695:Glycyrrhizic Acid / Q000737:chemistry; D006569:Hesperidin / Q000737:chemistry; D039823:Iridoids / Q000737:chemistry; D007529:Isoflavones / Q000737:chemistry; D017705:Lignans / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D039821:Monoterpenes; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000498:Allyl Compounds; D010636:Phenols; D006027:Glycosides
D000098:Acetophenones; D001565:Benzoates; D001713:Biphenyl Compounds; D001952:Bridged-Ring Compounds; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D019695:Glycyrrhizic Acid; D006569:Hesperidin; D039823:Iridoids; D007529:Isoflavones; D017705:Lignans; D039821:Monoterpenes; C516449:xiaoyao; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols; C007835:geniposide; C015423:peoniflorin; D006027:Glycosides; C013638:paeonol; C004742:daidzein; C004999:ferulic acid; C005274:naringin; C512196:liquiritin; C033607:puerarin