Proteomics study of the hepatitis C virus replication complex.
RNA replication of HCV occurs in the multiprotein complexes associated with the endoplasmic reticular (ER) membranes. The HCV NS3 to NS5B proteins are necessary and sufficient for HCV RNA replication in the cell, but cellular proteins in the HCV replication complex (RC) have not been determined. Several methods have been used to isolate the HCV RC, including crude cell extract preparation, subcellular fractionation, and affinity purification. The components of the HCV RC can be separated by two-dimensional electrophoresis and then determined by proteolytical digestion and mass spectrometry analysis in conjunction with peptide/protein database search and immunobiochemistry and functional genomic studies.
Chang
Kyungsoo
K
Department of Microbiology, Immunology and Molecular Genetics University of Kentucky, Lexington, KY, USA.
Wang
Tianyi
T
Luo
Guangxiang
G
D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D016174:Hepacivirus / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics; D012367:RNA, Viral / Q000096:biosynthesis*; D016415:Sequence Alignment; D014357:Trypsin / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins; D014357:Trypsin