Grapevine virusA-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera.
Virus-induced gene silencing (VIGS) is an attractive approach for studying gene function. Although the number of virus vectors available for use in VIGS experiments has increased in recent years, most of these vectors are applied in annual or herbaceous plants. The aim of this work was to develop a VIGS vector based on the Grapevine virus A (GVA), which is a member of the genus Vitivirus, family Flexiviridae. The GVA vector was used to silence the endogenous phytoene desaturase (PDS) gene in Nicotiana benthamiana plants. In addition, an Agrobacterium-mediated method for inoculating micropropagated Vitis vinifera cv. Prime plantlets via their roots was developed. Using this method, it was possible to silence the endogenous PDS gene in V. vinifera plantlets. The GVA-derived VIGS vector may constitute an important tool for improving functional genomics in V. vinifera.
Muruganantham
Mookkan
M
The S. Tolkowsky Laboratory, Department of Plant Pathology-The Virology Unit, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel.
Moskovitz
Yoni
Y
Haviv
Sabrina
S
Horesh
Tamar
T
Fenigstein
Annie
A
Preez
Jacques du
Jd
Stephan
Dirk
D
Burger
Johan T
JT
Mawassi
Munir
M
D052677:Flexiviridae / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D005818:Genetic Engineering; D005822:Genetic Vectors; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000821:virology; D030821:Plants, Genetically Modified; D012231:Rhizobium / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000821:virology*; D027843:Vitis / Q000235:genetics / Q000821:virology*
D010088:Oxidoreductases; C022398:phytoene dehydrogenase