Phoenito experiments: combining the strengths of commercial crystallization automation.
The use of crystallization robots for initial screening in macromolecular crystallization is well established. This paper describes how four general optimization techniques, growth-rate modulation, fine screening, seeding and additive screening, have been adapted for automation in a medium-throughput crystallization service facility. The use of automation for more challenging optimization experiments is discussed, as is a novel way of using both the Mosquito and the Phoenix nano-dispensing robots during the setup of a single crystallization plate. This dual-dispenser technique plays to the strengths of both machines.
Newman
Janet
J
CSIRO Division of Molecular and Health Technologies, 343 Royal Parade, Parkville, VIC 3052, Australia. janet.newman@csiro.au
Pham
Tam M
TM
Peat
Thomas S
TS
D001331:Automation / Q000295:instrumentation / Q000379:methods; D003460:Crystallization / Q000295:instrumentation / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000737:chemistry; D012371:Robotics / Q000295:instrumentation / Q000379:methods
D011506:Proteins