Transcriptome analysis of the zebrafish mind bomb mutant.
Mind bomb (Mib) facilitates Notch signaling pathway by promoting the endocytosis of Notch ligand. The zebrafish mib ( ta52b ) mutant has a defect in its ubiquitin ligase activity which is necessary to inhibit the neurogenesis, resulting in a neuronal hyperplasia. Several genes regulated in the mib ( ta52b ) mutant have been well established, however, there were relatively few reports about the transcriptome profile. To identify the genes differentially expressed in the mib ( ta52b ) mutant, genome-wide analysis was performed using serial analysis of gene expression. Three hundred and thirty-five transcripts were identified whose expressions were significantly altered in the mib ( ta52b ) mutant as compared with the wild-type. Interestingly, it was suggested that the mib ( ta52b ) mutation may affect not only neurogenesis but also mesoderm development. These results provide new insights into Notch signaling pathway.
Hwang
Junmo
J
School of Life Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.
Kim
Hyung-Soo
HS
Seok
Ji-Woong
JW
Kim
Jun-Dae
JD
Koun
Soonil
S
Park
So-Young
SY
Lee
Jinseok
J
Kim
Hyang Sook
HS
Kim
Hyung-Seok
HS
Kim
Kil Soo
KS
Chang
Kyu-Tae
KT
Ryoo
Zae Young
ZY
Wang
San Ming
SM
Huh
Tae-Lin
TL
Lee
Sanggyu
S
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D055106:Genome-Wide Association Study; D017403:In Situ Hybridization; D008648:Mesoderm / Q000196:embryology; D009154:Mutation; D055495:Neurogenesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D051880:Receptors, Notch; D029961:Zebrafish Proteins; D044767:Ubiquitin-Protein Ligases; C471932:mib1 protein, zebrafish