Influence of light intensity and selection scheme on regeneration time of transgenic flax plants.
This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic potentiality of calli (by a factor of 3.2) and accelerate the transgenic shoot regeneration (by a factor of about 2). On comparison of the transformation rate observed while using low light (LL) and high light (HL) a considerable enhancement from 0.12 to 5.7% was evident. The promotive effect of light might also had a direct beneficial effect on transgenic plant production time leading to a reduction of more than 4 months in the time need to obtain transgenic seeds. All data indicate that HL plays a role on growth and on protein, rubisco and pigment contents by stimulating the gene implicated in photosynthetic and Calvin cycle processes.
Caillot
Sébastien
S
Laboratoire de Génie Enzymatique et Cellulaire, UMR 6022 du CNRS, Université de Technologie de Compiègne, Compiègne Cedex, France.
Rosiau
Emeline
E
Laplace
Catherine
C
Thomasset
Brigitte
B
D003470:Culture Media; D019597:Flax / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects*; D018506:Gene Expression Regulation, Plant; D008027:Light; D010788:Photosynthesis / Q000235:genetics; D018520:Plant Shoots / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D018749:RNA, Plant / Q000378:metabolism; D012038:Regeneration; D014170:Transformation, Genetic
D003470:Culture Media; D018749:RNA, Plant