Photo-immobilization of biological components on gold-coated chips for measurements using surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM).
The photo-immobilization technique is useful for immobilization of various biomolecules on assorted material surfaces, independent of the organic functional groups that may be present. Here, we report a convenient new photo-immobilization technique that was developed by combining a nonbiofouling polymer containing polyethylene glycol and a photoreactive crosslinker for surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) measurements. By this method, nonspecific interactions were reduced and various types of molecules, bovine serum albumin, heparin, dsDNA, phosphatidylserine, Tobacco Mosaic Virus, and norfloxacine, were immobilized on an alkane thiol-modified gold surface by a single method. The interactions of photo-immobilized biomolecules and their corresponding antibodies were investigated by SPR and QCM. In addition, SPR imaging was possible using the present method.
Tsuzuki
Saki
S
Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Wada
Akira
A
Ito
Yoshihiro
Y
D003432:Cross-Linking Reagents; D004247:DNA / Q000032:analysis; D006046:Gold; D006493:Heparin / Q000032:analysis; D055767:Immobilized Proteins / Q000032:analysis*; D008689:Methacrylates; D046228:Microarray Analysis / Q000379:methods*; D010718:Phosphatidylserines / Q000032:analysis; D010777:Photochemistry / Q000379:methods; D011092:Polyethylene Glycols; D011108:Polymers; D011791:Quartz; D012710:Serum Albumin, Bovine / Q000032:analysis; D020349:Surface Plasmon Resonance / Q000379:methods*
D003432:Cross-Linking Reagents; D055767:Immobilized Proteins; D008689:Methacrylates; D010718:Phosphatidylserines; D011108:Polymers; C524499:polyethylene glycol methacrylate; D011791:Quartz; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D006046:Gold; D006493:Heparin; D004247:DNA