A novel amplification target, ARHGAP5, promotes cell spreading and migration by negatively regulating RhoA in Huh-7 hepatocellular carcinoma cells.
RhoA, a member of the Rho family of small GTPases, directs the organization of the actin cytoskeleton and is involved in regulating cell shape and movement. Its activity is negatively regulated by p190-B RhoGAP (GTPase-activating protein). We investigated DNA copy number aberrations in human hepatocellular carcinoma and esophageal squamous cell carcinoma cell lines using a high-density oligonucleotide microarray and found a novel amplification at chromosomal region 14q12. We identified ARHGAP5 (the gene encoding p190-B RhoGAP) as a probable target for the amplification at 14q12, and our results showed that p190-B RhoGAP promotes cells spreading and migration by negatively regulating RhoA activity in Huh-7 hepatocellular carcinoma cells.
Gen
Yasuyuki
Y
Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan.
Yasui
Kohichiroh
K
Zen
Keika
K
Nakajima
Tomoaki
T
Tsuji
Kazuhiro
K
Endo
Mio
M
Mitsuyoshi
Hironori
H
Minami
Masahito
M
Itoh
Yoshito
Y
Tanaka
Shinji
S
Taniwaki
Masafumi
M
Arii
Shigeki
S
Okanoue
Takeshi
T
Yoshikawa
Toshikazu
T
D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D002883:Chromosomes, Human, Pair 14 / Q000648:ultrastructure; D003599:Cytoskeleton / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D018450:Disease Progression; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008113:Liver Neoplasms / Q000378:metabolism*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
C513043:ARHGAP5 protein, human; D020690:GTPase-Activating Proteins; C066737:RHOA protein, human; D004247:DNA; D020742:rhoA GTP-Binding Protein