Cellular microRNA expression correlates with susceptibility of monocytes/macrophages to HIV-1 infection.
Although both monocytes and macrophages possess essential requirements for HIV-1 entry, peripheral blood monocytes are infrequently infected with HIV-1 in vivo and in vitro. In contrast, tissue macrophages and monocyte-derived macrophages in vitro are highly susceptible to infection with HIV-1 R5 tropic strains. We investigated intracellular anti-HIV-1 factors that contribute to differential susceptibility of monocytes/macrophages to HIV-1 infection. Freshly isolated monocytes from peripheral blood had significantly higher levels of the anti-HIV-1 microRNAs (miRNA, miRNA-28, miRNA-150, miRNA-223, and miRNA-382) than monocyte-derived macrophages. The suppression of these anti-HIV-1 miRNAs in monocytes facilitates HIV-1 infectivity, whereas increase of the anti-HIV-1 miRNA expression in macrophages inhibited HIV-1 replication. These findings provide compelling and direct evidence at the molecular level to support the notion that intracellular anti-HIV-1 miRNA-mediated innate immunity may have a key role in protecting monocytes/macrophages from HIV-1 infection.
Wang
Xu
X
Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Ye
Li
L
Hou
Wei
W
Zhou
Yu
Y
Wang
Yan-Jian
YJ
Metzger
David S
DS
Ho
Wen-Zhe
WZ
D004198:Disease Susceptibility / Q000821:virology; D015658:HIV Infections / Q000235:genetics*; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000821:virology*; D035683:MicroRNAs / Q000096:biosynthesis*; D009000:Monocytes / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014779:Virus Replication
D035683:MicroRNAs