Vitalisation of tubular coral scaffolds with cell sheets for regeneration of long bones: a preliminary study in nude mice.
In this study, cell sheets comprising multilayered living bone marrow stromal cells and extracellular matrix were assembled with tubular coral scaffolds for long bone regeneration. Cell sheet with visible mineralized nodules was harvested and wrapped against tubular coral scaffolds with 5mm diameter and 1.5mm wall thickness. New bone formation was investigated by CT scan and histological observation 8 and 12 weeks after implantation of cell sheet/scaffold. The results showed that cortical bone formed within the constructs for both groups. New bone composed 25.75% of the graft in 8 weeks group, compared to that of 40.01% in 12 weeks group. Histological examination showed that new bone formation was in the manner of endochondral osteogenesis, with woven bone matrix subsequently maturing into fully mineralized compact bone. These findings demonstrated that osteogenic cell sheet could vitalize tubulate coral scaffolds to regenerate bone graft with similar shape and structure to native bone.
Gao
Zhan
Z
Department of Oral &Maxillofacial Surgery, Qin Du Hospital, School of Stomatology, The Fourth Military Medical University, Chang Le Xi Road 146#, Xi'an 710032, PR China.
Chen
Fulin
F
Zhang
Junrui
J
He
Lisheng
L
Cheng
Xiaobin
X
Ma
Qin
Q
Mao
Tianqiu
T
D000818:Animals; D001857:Bone Matrix / Q000637:transplantation; D001861:Bone Regeneration; D002119:Calcium Carbonate; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000637:transplantation; D005269:Femur; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D011817:Rabbits; D017154:Stromal Cells / Q000637:transplantation; D040521:Subcutaneous Tissue; D054457:Tissue Scaffolds
D002119:Calcium Carbonate