title: Biofilm formation by persistent and non-persistent isolates of Staphylococcus epidermidis from a neonatal intensive care unit.
abstract: Correlation of biofilm formation and presence of the icaADBC genes was studied in 161 clinical isolates of S. epidermidis from persistent and non-persistent neonatal infections. Biofilm formation was compared in trypticase soy broth with or without added glucose (0.5-9.23%), and total parenteral nutrition (TPN) containing 9.23% glucose. Detection of icaADBC genes was carried out using polymerase chain reaction and ica-specific primers. Quantitative biofilm formation for all isolates was highest in the presence of 1% glucose, followed by trypticase soy broth and TPN. There was no significant difference between the amount of biofilm formed by persistent and non-persistent isolates under different test conditions. In contrast, 70% of the persistent isolates produced biofilm in TPN compared to the 56.3% of the non-persistent group. Neither the persistent bacterial phenotype nor presence of the icaADBC operon was correlated with biofilm formation.
authors.affiliation: Microbiology Department, Faculty of Biological Sciences, Shahid Beheshti University, Evin, Tehran, Iran. f-eftekhar@sbu.ac.ir
authors.forename: F
authors.initials: F
authors.lastname: Eftekhar
authors.forename: D P
authors.initials: DP
authors.lastname: Speert
mesh_terms: D018441:Biofilms; D003428:Cross Infection / Q000235:genetics / Q000382:microbiology; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D009876:Operon / Q000235:genetics*; D010289:Parenteral Nutrition, Total; D011135:Polysaccharides, Bacterial / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000235:genetics* / Q000378:metabolism
chemical_list: D011135:Polysaccharides, Bacterial; C113579:polysaccharide intercellular adhesin; D005947:Glucose