title: Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from the extremophilic microorganism Bacillus thermocatenulatus.
abstract: Bacillus thermocatenulatus lipase 2 (BTL2) is a thermoalkalophilic lipase that has been reported as an enantioselective biocatalyst for diverse reactions and that heads a group of enzymes that share high resistance towards many inactivation agents (heat, organic solvents, pH etc.). This makes BTL2 an important research target because of its potential industrial applications. BTL2 was cloned and overexpressed in Escherichia coli, purified and concentrated for crystallization using the sitting-drop vapour-diffusion method at 291 K. Crystals grew from a mixture of 13% MPD and 0.2 M ammonium acetate in 0.05 M sodium citrate pH 5.5-5.6. The crystals, which belonged to the orthorhombic space group I222 with unit-cell parameters a = 73.07, b = 129.08, c = 127.49 A, allowed the collection of an X-ray data set to 2.2 A resolution.
authors.affiliation: Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto de Química-Física Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain.
authors.forename: César
authors.initials: C
authors.lastname: Carrasco-López
authors.forename: Cesar
authors.initials: C
authors.lastname: Godoy
authors.forename: Blanca
authors.initials: B
authors.lastname: de las Rivas
authors.forename: Gloria
authors.initials: G
authors.lastname: Fernández-Lorente
authors.forename: José M
authors.initials: JM
authors.lastname: Palomo
authors.forename: José M
authors.initials: JM
authors.lastname: Guisán
authors.forename: Roberto
authors.initials: R
authors.lastname: Fernández-Lafuente
authors.forename: Martín
authors.initials: M
authors.lastname: Martínez-Ripoll
authors.forename: Juan A
authors.initials: JA
authors.lastname: Hermoso
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D008049:Lipase / Q000737:chemistry*; D008969:Molecular Sequence Data; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D008049:Lipase