An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening.
Glucuronic acid is a common chemical moiety that decorates the xylan polymer of hemicellulose. This chemical substituent impairs both enzymatic and acidic hydrolysis of xylosidic bonds. The alpha-glucuronidase enzyme hydrolyzes the 1,2-linked glucuronic acid from the terminal, non-reducing xylose of xylo-oligosaccharides. There are relatively few alpha-glucuronidase genes in the public databases. We have developed an assay with commercially available reagents that can be used to search DNA libraries for alpha-glucuronidase genes in a high-throughput, solid phase activity screen.
Lee
Charles C
CC
USDA-ARS, Albany, CA 94710, USA. CLEE@pw.usda.gov
Wagschal
Kurt
K
Kibblewhite-Accinelli
Rena E
RE
Orts
William J
WJ
Robertson
George H
GH
Wong
Dominic W S
DW
D001681:Biological Assay / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D020723:Glucuronic Acid / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*
D020723:Glucuronic Acid; D006026:Glycoside Hydrolases; C096734:alpha-glucuronidase