Specific expression of short-interfering RNA driven by human telomerase reverse transcriptase promoter in tumor cells.
RNA interference (RNAi) has been shown to be an effective method for inhibiting the expression of a given gene in human cells by targeting with short duplex RNA (short-interfering RNA or siRNA). However, more and more studies suggest that non-specific effects can be induced by siRNAs, such as off-target inhibition, activation of interferon response, and saturation of cellular silencing machinery. It has been known that more than 90% of human tumors exhibit telomerase activity. Consequently, telomerase is believed to be a broad-spectrum molecular marker of malignancies. In the present study we attempt to develop a tumor-specific RNAi system using the human telomerase reverse transcriptase promoter. This system may provide a basis for RNAi therapy.
Luan
Xuejing
X
Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin 300070, China.
Guo
Limin
L
Yang
Zuozhen
Z
Liu
Min
M
Li
Xin
X
Tang
Hua
H
D001483:Base Sequence; D002460:Cell Line; D045744:Cell Line, Tumor; D017931:DNA Primers; D017930:Genes, Reporter; D006801:Humans; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics*
D017931:DNA Primers; D034741:RNA, Small Interfering; D019098:Telomerase