[Effects of tamoxifen on apoptosis and matrix metalloproteinase-7 expression in estrogen receptor beta-positive colonic cancer cell line HT-29].
OBJECTIVE
Studies have shown that estrogen receptor beta (ERbeta), which is highly expressed in colorectal cancer, is related to tumor metastasis. Matrix metalloproteinase (MMP)-7 is overexpressed in colorectal cancer, and plays an important role in tumor invasion and metastasis. This study was to explore the effect of tamoxifen (TAM) on apoptosis and MMP-7 expression in ERbeta-positive human colorectal cancer cell line HT-29.


METHODS
After exposure to TAM, the proliferation of HT-29 cells was detected by MTT assay; cell apoptosis was evaluated using flow cytometry; expressions of ERbeta and MMP-7 were measured by Western blot.


RESULTS
TAM significantly inhibited cell growth of HT-29 in a time (24 h, 48 h, 72 h )-and dose (0, 10(-7), 10(-6), 10(-5),0 10(-4) mol/L )-dependent manner. TAM exposure caused significant cell apoptosis of HT-29 cells at the concentration of 10(-4) mol/L [(69.9+/-4.2)%]. Moreover, TAM could bind to ERbeta to down-regulate MMP-7 protein expression in HT-29 cells.


CONCLUSIONS
High concentration of TAM can inhibit the proliferation of ERbeta-positive HT-29 cells, and effectively bind with ERbeta to down-regulate MMP-7 expression.
Fang
Yu-Jing
YJ
State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, China.
Wang
Guo-Qiang
GQ
Lu
Zhen-Hai
ZH
Zhang
Li-Yi
LY
Pan
Zhi-Zhong
ZZ
Zhou
Zhi-Wei
ZW
Wan
De-Sen
DS
D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation; D047629:Estrogen Receptor beta / Q000378:metabolism*; D019073:HT29 Cells; D006801:Humans; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*; D013629:Tamoxifen / Q000494:pharmacology*
D018931:Antineoplastic Agents, Hormonal; D047629:Estrogen Receptor beta; D013629:Tamoxifen; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7