Glycogen synthase kinase-3beta is associated with Parkinson's disease.
We immunohistochemically examined the expression of glycogen synthase kinase-3beta (GSK-3beta) in the brains of Parkinson's disease (PD) patients. GSK-3beta was localized in punctate structures in the cytosol of subsets of neurons in the midbrain and upper pons. GSK-3beta was also localized in Lewy bodies (LBs) as was phosphorylated GSK-3beta (Ser9) (pGSK-3beta (Ser9)). Both GSK-3beta and pGSK-3beta (Ser9) were localized specifically in the halo of LBs. The core of LBs was negative for GSK-3beta, while pGSK-3beta (Ser9) was present in only a small number of LB cores. Cortical LBs were positive for pGSK-3beta (Ser9) but not for GSK-3beta. Neither GSK-3beta nor pGSK-3beta (Ser9) was present in glial cytoplasmic inclusions (GCIs) in the brains of multiple system atrophy (MSA) patients. Our results suggest that GSK-3beta plays a role in the pathogenesis of PD but not in that of MSA.
Nagao
Masahiro
M
Department of Neurology, Tokyo Metropolitan Neurological Hospital, Tokyo, Japan. nagaom@tmnh.fuchu.tokyo.jp
Hayashi
Hideaki
H
D000368:Aged; D000595:Amino Acid Sequence / Q000502:physiology; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001921:Brain / Q000201:enzymology* / Q000473:pathology* / Q000503:physiopathology; D003600:Cytosol / Q000201:enzymology / Q000473:pathology; D005260:Female; D038362:Glycogen Synthase Kinase 3 / Q000032:analysis / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000201:enzymology / Q000473:pathology; D016631:Lewy Bodies / Q000201:enzymology / Q000473:pathology; D008297:Male; D008636:Mesencephalon / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D019578:Multiple System Atrophy / Q000201:enzymology / Q000473:pathology; D009474:Neurons / Q000201:enzymology / Q000473:pathology; D010300:Parkinson Disease / Q000201:enzymology* / Q000473:pathology* / Q000503:physiopathology; D010766:Phosphorylation; D011149:Pons / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D012694:Serine / Q000378:metabolism
D015415:Biomarkers; D012694:Serine; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3