Analysis of hollow-core photonic bandgap fibers for evanescent wave biosensing.
Hollow-core photonic bandgap fiber (HC-PBGF)-based evanescent wave biosensors are demonstrated and analyzed theoretically and experimentally. With 95% of the guided light power residing in the samples, the measured absorbance for a 30-cm-long fiber filled with a 0.2 microM Alexa Fluor 700-labeled DNA Oligo solution is 1.06. This is in good agreement with the theoretical prediction, which is evaluated by using the refractive index scaling law. The HC-PBGFs thus offer both efficiency and simplicity for the detection of biomolecules in ultra-small sample volumes.
Sun
Jian
J
Nanyang Technological University, School of Chemical and Biomedical Engineering, Division of Bioengineering, Singapore, 637457.
Chan
Chi-Chiu
CC
Zhang
Yi-Fan
YF
Shum
Ping
P
D001704:Biopolymers / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D017076:Computer-Aided Design; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D017785:Photons; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*
D001704:Biopolymers; D004247:DNA