Effects of formaldehyde inhalation on the junctional proteins of nasal respiratory mucosa of rats.
Exposure to formaldehyde, which is an organic compound, disturbs the integrity of nasal mucosa. In this study, we aimed to clarify the protein changes in the junctional complex of nasal mucosa of Wistar rats exposed to formaldehyde inhalation. The study was performed in 20 female Wistar rats. Rats were divided into two groups randomly. Control rats were allowed free access to standard rat chaw and tap water (n:10). Experimental group was exposed to formaldehyde vapor at 15ppm, 6h/day, 5 days/week for 12 weeks (n:10). Histological evaluation of the experimental model was determined by hematoxylin-eosin (HE) and periodic acid Schiff (PAS) stainings of paraffin-embedded nasal mucosa tissues and by electron microscopy. The effects of formaldehyde inhalation on the distribution of occludin, E-cadherin, and gamma-catenin were assessed by immunohistochemistry. The nasal mucosa of the experimental group was correlated with hypertrophy in goblet cell, degeneration in basal lamina, stratification of epithelium, and proliferation. Thickness of basal lamina and also local degenerative regions, vacuole increase in cytoplasmic areas, irregular forms of kinocilium and loss of sharpness in the kinocilium membrane were the findings at the ultrastructural level. The expressions of E-cadherin, occludin, gamma-catenin proteins in intercellular junctional complexes of rat nasal mucosa were also decreased in experimental group compared to control group. The findings of the present study indicated that formaldehyde vapor inhalation in the concentrations and duration of exposure used in the present experiment significantly decreased the density of structural proteins of the junctional complex in the nasoepithelium. It was suggested that, the formaldehyde inhalation could cause complete impairment of intercellular junctional complexes and disturb the tissue integrity in nasal mucosa at higher concentrations.
Arican
R Yavuz
RY
Department of Anatomy, Faculty of Medicine, Antalya, Turkey.
Sahin
Zeliha
Z
Ustunel
Ismail
I
Sarikcioglu
Levent
L
Ozdem
Sadi
S
Oguz
Nurettin
N
D022005:Adherens Junctions / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D003896:Desmosomes / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005557:Formaldehyde / Q000633:toxicity*; D017629:Gap Junctions / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D019570:Inhalation Exposure / Q000009:adverse effects*; D007365:Intercellular Junctions / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008565:Membrane Proteins / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009297:Nasal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D062793:Occludin; D051381:Rats; D017208:Rats, Wistar; D051185:gamma Catenin / Q000378:metabolism*
D015820:Cadherins; D008565:Membrane Proteins; D062793:Occludin; C568987:Ocln protein, rat; D051185:gamma Catenin; D005557:Formaldehyde