Morphological and morphometrical alterations of the osteochondral junction in perinatal necropsies.
Infants who die during the perinatal period could present the following upon examination of the ribs: alterations of the osteochondral junction (OCJ) that could be related to intrauterine growth restriction, placental alterations, maternal disorders, and congenital abnormalities. The aim of this study was to identify the morphological alterations of the OCJ in the autopsied infants and the factors associated with its pathogenesis. The OCJ from 254 infants were sequentially autopsied and analyzed. Hematoxylin-eosin and blue Masson's trichrome stains were used for examination. The expression in the chondrocytes of the cartilage oligomeric matrix protein (COMP) was measured using a polyclonal antibody. There were 199 (78.3%) cases with normal OCJ and 55 (22%) cases with alterations; among these, 38 (14.9%) cases have an increased cartilage matrix at the free bone marrow zone of the OCJ (ICM), 10 (3.9%) cases have a bizarre pattern (BZ), 5 (2%) cases have a bone tissue formation closing de growth channels (MCO) of the OCJ, and 2 (0.8%) cases have bone marrow cells encroaching the free bone marrow zone. The length of the proliferative zone was different in the groups with alterations of the OCJ (P < .001), being higher in the group of patients with MCO and ICM (P < .05). In the group with BZ, the length was smaller (P < .05). The analysis of the OCJ is important in the autopsies performed at the perinatal period, and this study contributes for a better understanding of the mechanisms related to the etiology of these alterations.
Salge
Ana Karina Marques
AK
Nursing School, Goiás Federal University, Goiânia, Brazil. anakarina@fen.ufg.br
Evangelista
Tarcísio Barcelos
TB
Canno
Rogério Carrijo
RC
Bernardino
Pricila
P
Franco
Nátila Alves
NA
Santos
Renata Pereira Paes
RP
e Silva
Renata Calciolari Rossi
RC
Côrrea
Rosana Rosa Miranda
RR
Guimarães
Janaína Valadares
JV
Peres
Luiz César
LC
Castro
Eumenia Costa da Cunha
EC
D001344:Autopsy; D001853:Bone Marrow / Q000473:pathology; D002356:Cartilage / Q000473:pathology; D064236:Cartilage Oligomeric Matrix Protein; D049109:Cell Proliferation; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D064235:Matrilin Proteins; D010012:Osteogenesis; D012189:Retrospective Studies; D012272:Ribs / Q000473:pathology*; D050497:Stillbirth
D064236:Cartilage Oligomeric Matrix Protein; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; C577553:TSP5 protein, human