Role of viral induced vascular endothelial growth factor (VEGF) production in pleural effusion and malignant mesothelioma.
Vascular endothelial growth factor (VEGF) plays a key role in formation of pleural effusions and in tumorigenesis and progression of malignant mesothelioma. Mesothelial cells (MC) express the viral receptors Toll-like receptor 3 (TLR3), RIG-I and MDA5. Activation of these receptors by viral RNA exemplified by poly(I:C) RNA leads to a time- and dose-dependent increase of mesothelial VEGF synthesis. To show the specific effect of viral receptors knockdown experiments with siRNA for TLR3, RIG-I and MDA5 were performed. This finding of viral induced mesothelial VEGF synthesis may indicate a novel link between viral infections and formation of pleural effusions and progression of malignant mesothelioma.
Wörnle
Markus
M
Medical Policlinic, Ludwig-Maximilians-University, Munich, Germany. markus.woernle@med.uni-muenchen.de
Sauter
Matthias
M
Kastenmüller
Kathrin
K
Ribeiro
Andrea
A
Roeder
Maximilian
M
Mussack
Thomas
T
Ladurner
Roland
R
Sitter
Thomas
T
D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D006801:Humans; D008654:Mesothelioma / Q000378:metabolism* / Q000821:virology; D016066:Pleural Effusion, Malignant / Q000378:metabolism* / Q000821:virology; D011070:Poly I-C / Q000494:pharmacology; D034741:RNA, Small Interfering; D012367:RNA, Viral / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000378:metabolism*
D034741:RNA, Small Interfering; D012367:RNA, Viral; D042461:Vascular Endothelial Growth Factor A; D011070:Poly I-C