Recording the activity of ATP-sensitive K(+) channels in open-cell cell-attached configuration.
The functional activity of ion channels is affected by their local environment, and for certain types of channels this local effect is critical. This chapter describes a method of recording the activity of ATP-sensitive K(+) channels, expressed on the plasma membrane of pancreatic beta-cells, without destroying the beta-cell architecture. In the open-cell cell-attached patch-clamp configuration, ligands and effectors of these metabolism-dependent channels are delivered to their cytoplasmic side via pores in the plasma membrane produced by bacterial toxins.
Tarasov
Andrei I
AI
Section of Cell Biology, Division of Medicine, Imperial College London, UK.
D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology; D001427:Bacterial Toxins / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004594:Electrophysiology / Q000379:methods; D006460:Hemolysin Proteins / Q000494:pharmacology; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D054086:KATP Channels / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D018408:Patch-Clamp Techniques; D013301:Streptolysins / Q000494:pharmacology
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D054086:KATP Channels; D013301:Streptolysins; C021091:staphylococcal alpha-toxin; C025964:streptolysin O