Apicidin decreases phospholipase C gamma-1 transcript and protein in Hut-78 T lymphoma cells.
Phospholipase C gamma-1 (PLCgamma1) phosphorylation is a key step in intracellular signal transduction in T cells. We used Hut-78 T lymphoma cells to demonstrate the effect of apicidin on cellular levels of the PLCgamma1 molecule. Using reverse-transcription, real-time quantitative PCR and Western blot analysis, we observed that apicidin reduced the PLCgamma1 transcript and protein contents in Hut-78 T lymphoma cells. Our results indicate that protein synthesis appears to be crucial in the apicidin decrease of PLCgamma1 mRNA steadiness. Moreover, we determined that apicidin reduces the half-life of PLCgamma1 mRNAs from approximately 2 to 4h. Since PLCgamma1 is considered a key molecule in signal transduction in T cells, apicidin may be useful in the treatment of some autoimmune diseases in which autoreactive T cells occur.
Debicki
Szymon
S
Poznań University of Medical Sciences, Department of Biochemistry and Molecular Biology, 60-781 Poznań, Poland.
Jagodzinski
Paweł P
PP
D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D056572:Histone Deacetylase Inhibitors; D016399:Lymphoma, T-Cell; D010456:Peptides, Cyclic / Q000494:pharmacology*; D051966:Phospholipase C gamma / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits; D015398:Signal Transduction / Q000235:genetics*
D056572:Histone Deacetylase Inhibitors; D010456:Peptides, Cyclic; D012333:RNA, Messenger; C102351:apicidin; D051966:Phospholipase C gamma