title: Immune response and alveolar bone resorption in a mouse model of Treponema denticola infection.
abstract: Treponema denticola is considered to be an agent strongly associated with periodontal disease. The lack of an animal infection model has hampered the understanding of T. denticola pathogenesis and the host's immune response to infection. In this study, we have established an oral infection model in mice, demonstrating that infection by oral inoculation is feasible. The presence of T. denticola in the oral cavities of the animals was confirmed by PCR. Mice given T. denticola developed a specific immune response to the bacterium. The antibodies generated from the infection were mainly of the immunoglobulin G1 subclass, indicating a Th2-tilted response. The antibodies recognized 11 T. denticola proteins, of which a 62-kDa and a 53-kDa protein were deemed immunodominant. The two proteins were identified, respectively, as dentilisin and the major outer sheath protein by mass spectrometry. Splenocytes cultured from the infected mice no longer produced interleukin-10 and produced markedly reduced levels of gamma interferon relative to those produced by naïve splenocytes upon stimulation with T. denticola. Mandibles of infected mice showed significantly greater bone resorption (P < 0.01) than those of mock-infected controls.
authors.affiliation: Department of Applied Oral Sciences, Faculty of Dentistry, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5. Song.Lee@Dal.Ca
authors.forename: Song F
authors.initials: SF
authors.lastname: Lee
authors.forename: Elisoa
authors.initials: E
authors.lastname: Andrian
authors.forename: Elden
authors.initials: E
authors.lastname: Rowland
authors.forename: Ignacio Christian
authors.initials: IC
authors.lastname: Marquez
mesh_terms: D016301:Alveolar Bone Loss / Q000276:immunology* / Q000382:microbiology*; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D002918:Chymotrypsin / Q000737:chemistry / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D009059:Mouth Diseases / Q000276:immunology / Q000382:microbiology; D010447:Peptide Hydrolases; D018272:Porins / Q000737:chemistry / Q000276:immunology; D048373:Treponema denticola / Q000276:immunology*; D014211:Treponemal Infections / Q000276:immunology* / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D018272:Porins; C099361:major outer sheath protein, Treponema; D010447:Peptide Hydrolases; C102570:dentilisin; D002918:Chymotrypsin