Chickpea protein hydrolysate as a substitute for serum in cell culture.
The growth of mammalian cells in vitro requires the use of rich culture media that are prepared by combining serum with specific nutrient formulations. Serum, the most expensive component of culture media, provides a complex mixture of growth factors and nutrients. Protein hydrolysates that can support in vitro cell growth and eliminate or reduce the need to use serum have been obtained from different sources. Here we describe the use of two food grade proteases to produce a chickpea protein hydrolysate that has been added to cell culture medium in order to determine whether it can be used as a substitute for serum. Medium containing the hydrolysate has been tested using two human cells lines: the monocytic THP-1 cell line which grows in suspension, and the epithelial Caco-2 cell line which grows as a monolayer. The chickpea protein hydrolysate was a good substitute for serum in the first case, but did not allow growth of Caco-2 cells. Supplementation of culture media with this inexpensive and safe hydrolysate would greatly reduce the cost of cell culture.
Girón-Calle
Julio
J
Instituto de la Grasa (Consejo Superior de Investigaciones Científicas), Avenida Padre García Tejero, 4, 41012, Sevilla, Spain, jgiron@cica.es.
Vioque
Javier
J
Pedroche
Justo
J
Alaiz
Manuel
M
Yust
María M
MM
Megías
Cristina
C
Millán
Francisco
F