An attempt to reveal a role of a transcription/translation feedback loop in the cyanobacterial KaiC protein-based circadian system by using a semi-synthetic method.
The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The synthetic approach is also useful in systems biology in that it can be used to create artificial bypasses for processes related to cellular phenomena of interest for their easier analysis. To validate the role of transcription feedback in the cyanobacterial circadian system, we propose an experimental design for a 'semi-synthetic' approach that involves transplantation of the kaiABC genes into Escherichia coli and the construction of chimeric transcriptional outputs. The design principle and preliminary results are discussed.
Tozaki
Hirokazu
H
ERATO Aihara Complexity Modelling Project, JST, 3-23-5 Uehara, Shibuya, Tokyo 151-0064, Japan. htozaki@aihara.jst.go.jp
Kobe
Taiichiro
T
Aihara
Kazuyuki
K
Iwasaki
Hideo
H
D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology; D056925:Circadian Rhythm Signaling Peptides and Proteins; D019295:Computational Biology; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D025461:Feedback, Physiological; D005798:Genes, Bacterial; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D010770:Phosphotransferases / Q000235:genetics / Q000502:physiology; D014176:Protein Biosynthesis; D015398:Signal Transduction; D046940:Synechococcus / Q000235:genetics* / Q000502:physiology*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D056925:Circadian Rhythm Signaling Peptides and Proteins; C114271:KaiC protein, cyanobacteria; D010770:Phosphotransferases; C083143:SasA protein, Synechococcus