Cuprizone treatment induces demyelination and astrocytosis in the mouse hippocampus.
Memory impairment is outstanding within the spectrum of cognitive deficits in multiple sclerosis (MS) patients. Demyelination has been reported in the hippocampus formation of MS patients. The degree of hippocampus lesions in MS strongly correlates with progression of cognitive dysfunction. Because no appropriate animal model for the study of hippocampus demyelination has been established, we used the cuprizone mouse model to investigated demyelination in young adult and aged mice. The myelin status was analyzed by classical histological staining, immunocytochemistry for proteolipoprotein, and electron microscopy. Oligodendrocyte, astroglial, and microglia markers were studied. Cuprizone intoxication induced an almost complete demyelination of distinct hippocampus subregions to a similar extent in young adult and aged male mice. Demyelination was pronounced in a subset of white and gray matter areas, i.e., the stratum lacunosum moleculare containing the perforant path, medial alveus, stratum pyramidale in the cornu ammonis 2/3 region, and hilus region. Besides demyelination, affected areas displayed hypertrophic and hyperplastic astrocytosis. No significant effect on microglia invasion was detected at any investigated time point (0, 3, 5, and 7 weeks). We conclude that cuprizone-induced demyelination provides an adequate animal model to investigate appropriate therapy strategies for the prevention of hippocampus demyelination.
Norkute
Akvile
A
Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Aachen, Germany.
Hieble
Andrea
A
Braun
Alena
A
Johann
Sonja
S
Clarner
Tim
T
Baumgartner
Werner
W
Beyer
Cordian
C
Kipp
Markus
M
D000375:Aging; D000818:Animals; D001253:Astrocytes / Q000473:pathology; D003471:Cuprizone / Q000494:pharmacology*; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000473:pathology*; D004195:Disease Models, Animal; D005911:Gliosis / Q000139:chemically induced / Q000473:pathology; D006624:Hippocampus / Q000473:pathology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D007211:Indoles; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000473:pathology; D008854:Microscopy, Electron; D009103:Multiple Sclerosis; D009186:Myelin Sheath / Q000648:ultrastructure; D009836:Oligodendroglia / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007211:Indoles; C018588:Luxol Fast Blue MBS; D003471:Cuprizone