title: NDE1 and NDEL1: multimerisation, alternate splicing and DISC1 interaction.
abstract: Nuclear Distribution Factor E Homolog 1 (NDE1) and NDE-Like 1 (NDEL1) are highly homologous mammalian proteins. However, whereas NDEL1 is well studied, there is remarkably little known about NDE1. We demonstrate the presence of multiple isoforms of both NDE1 and NDEL1 in the brain, showing that NDE1 binds directly to multiple isoforms of Disrupted in Schizophrenia 1 (DISC1), and to itself. We also show that NDE1 can complex with NDEL1. Together these results predict a high degree of complexity of DISC1-mediated regulation of neuronal activity.
authors.affiliation: The Centre for Molecular Medicine, Western General Hospital, The University of Edinburgh, Edinburgh EH4 2XU, UK.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Bradshaw
authors.forename: Sheila
authors.initials: S
authors.lastname: Christie
authors.forename: Dinesh C
authors.initials: DC
authors.lastname: Soares
authors.forename: Becky C
authors.initials: BC
authors.lastname: Carlyle
authors.forename: David J
authors.initials: DJ
authors.lastname: Porteous
authors.forename: J Kirsty
authors.initials: JK
authors.lastname: Millar
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002461:Cell Line, Transformed; D002522:Chlorocebus aethiops; D004843:Epistasis, Genetic / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014162:Transfection
chemical_list: D002352:Carrier Proteins; C412476:DISC1 protein, human; D008869:Microtubule-Associated Proteins; C421108:NDEL1 protein, human; C525094:Nde1 protein, human; D009419:Nerve Tissue Proteins; D049452:Green Fluorescent Proteins