title: A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.
abstract: We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent studies focused on the infection, virus production and harvest from suspension culture bioreactors. Future studies are planned to address the kinetics of adenovirus production in HEK 293 as well as in other cell lines.
authors.affiliation: Fermentation and Cell Culture Development, Biotechnology R&D (CMC), Berlex Biosciences, 15409 San Pablo Avenue, Richmond, CA, 94804, U.S.A.
authors.forename: G
authors.initials: G
authors.lastname: Schoofs
authors.forename: T J
authors.initials: TJ
authors.lastname: Monica
authors.forename: J
authors.initials: J
authors.lastname: Ayala
authors.forename: J
authors.initials: J
authors.lastname: Horwitz
authors.forename: T
authors.initials: T
authors.lastname: Montgomery
authors.forename: G
authors.initials: G
authors.lastname: Roth
authors.forename: F J
authors.initials: FJ
authors.lastname: Castillo