Sweet silver: a formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry.
Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features, but its compatibility with MS is limited. We describe here, a new variant of silver staining that is completely formaldehyde-free. Reducing sugars in alkaline borate buffer are used as developers. While keeping the benefits of silver staining, this method is shown to afford a much better performance in terms of compatibility with MS, both in PMF by MALDI and in LC/ESI/MS/MS.
Chevallet
Mireille
M
CEA-Laboratoire de Biochimie et Biophysique des systèmes Intégrés, iRTSV/BBSI, CEA-Grenoble, Grenoble, France.
Luche
Sylvie
S
Diemer
Hélène
H
Strub
Jean-Marc
JM
Van Dorsselaer
Alain
A
Rabilloud
Thierry
T
D000447:Aldehydes / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D005557:Formaldehyde / Q000737:chemistry*; D008291:Malate Dehydrogenase / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D016622:Silver Staining / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016596:Vinculin / Q000032:analysis / Q000737:chemistry
D000447:Aldehydes; D016596:Vinculin; D005557:Formaldehyde; D008291:Malate Dehydrogenase