Development of functional lab-on-a-chip on polymer for point-of-care testing of metabolic parameters.
This paper presents the development of an easy-to-handle and disposable clinical diagnostic lab-on-a-chip using fully integrated plastic microfluidic components, which has the sampling/identifying capability to make fast and reliable measurements of metabolic parameters from human whole blood. A smart and functional lab-on-a-chip cartridge, which incorporates a full on-chip auto-calibration function for in the field applications, has been developed, and then fully characterized using a portable analyzer (3 (1/4)''x 5''x 1'') with multi-analyte detection capability. In addition, several new approaches in realizing smart and functional lab-on-a-chips on polymer have been adopted, which include the pinch valve for automatic fluidic sealing, a by-pass channel as the sampling indicator, and a robust connector design for long analyzer lifetimes. Metabolic parameters such as glucose, lactate, and partial oxygen from human whole blood have been successfully measured using the functional polymer lab-on-a-chips and the portable analyzer developed in this work.
Do
Jaephil
J
Electrical and Computer Engineering, University of Cincinnati, Cincinnati, OH 45221, USA. dowooner@hotmail.com
Lee
Sehwan
S
Han
Jungyup
J
Kai
Junhai
J
Hong
Chien-Chong
CC
Gao
Chuan
C
Nevin
Joseph H
JH
Beaucage
Gregory
G
Ahn
Chong H
CH
D001774:Blood Chemical Analysis; D001786:Blood Glucose / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D007773:Lactates / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D046208:Microchip Analytical Procedures / Q000379:methods*; D019095:Point-of-Care Systems
D001786:Blood Glucose; D007773:Lactates; D019344:Lactic Acid