Selection of transformed plants.
The low frequency and randomness of transgene integration into host cells, combined with the significant challenges of recovering whole plants from those rare events, makes the use of selectable marker genes routine in plant transformation experiments. For research applications that are unlikely to be grown in the field, strong herbicide- or antibiotic resistance is commonly used. Here we use genes conferring resistance to glufosinate herbicides as an example of a selectable marker in wheat transformation by either Agrobacterium or biolistics.
Jones
Huw D
HD
Department of Plant Sciences, Rothamsted Research, Centre for Crop Genetic Improvement, Harpenden, Hertfordshire, UK.
Sparks
Caroline A
CA
D000613:Aminobutyrates / Q000494:pharmacology; D018554:Avena / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D019470:Biolistics; D004351:Drug Resistance / Q000235:genetics; D017343:Genes, Plant; D005818:Genetic Engineering / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology; D001467:Hordeum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D063245:Plant Development; D010944:Plants / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified; D012231:Rhizobium / Q000378:metabolism; D014908:Triticum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000382:microbiology
D000613:Aminobutyrates; D005819:Genetic Markers; D006540:Herbicides; C003121:phosphinothricin