title: [Mouse model of experimental antoimmune encephalomyelitisin C57BL/6J and expression of macrophage migration inhibitory factor].
abstract: OBJECTIVE
  To detect the expression of macrophage migration inhibitory factor(MIF) in the brain and spinal cord of chronic non-remitting model of experimental autoimmune encephalomyelitis(EAE) mouse, and to discuss their effect on EAE/MS.
  
  
  METHODS
  Seventy-two female SPF C57BL/6J mice, aged 6k8 weeks, were randomly divided into an EAE group, a blank group, and an adjuvant group. The mice were immuned by mMOG35-55 and CFA. Immunohistochemic technique was used to detect the expression of MIF in the brain and spinal cord.
  
  
  RESULTS
  In the EAE group, we observed up-regulation of MIF of central nervous system(CNS) at onset, peak and chronic phase. During each phase, the difference of MIF between the EAE group and each of the other 2 groups was significant (P<0.05). In the EAE group, the expression of MIF was the highest at the peak, which was different from other periods (P<0.05).
  
  
  CONCLUSIONS
  MIF significanty expressed during the procedure of EAE disease, and may be related with the onset and exacerbation of EAE.
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yun-chun
authors.initials: YC
authors.lastname: Gao
authors.forename: Yu-zhong
authors.initials: YZ
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Jin-jin
authors.initials: JJ
authors.lastname: Yan
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000378:metabolism*; D005260:Female; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D015854:Up-Regulation
chemical_list: D008263:Macrophage Migration-Inhibitory Factors