A large kindred with X-linked neutropenia with an I294T mutation of the Wiskott-Aldrich syndrome gene.
X-linked neutropenia (XLN, OMIM #300299) is a rare form of severe congenital neutropenia. It was originally described in a three-generation family with five affected members that had an L270P mutation in the GTP-ase binding domain (GBD) of the Wiskott-Aldrich syndrome protein (WASP) [Devriendt et al (2001) Nature Genetics, Vol. 27, 313-317]. Here, we report and describe a large three-generation family with XLN, with 10 affected males and eight female carriers. A c.882T>C mutation was identified in the WAS gene, resulting in an I294T mutation. The infectious course is variable and mild in view of the profound neutropenia. In addition to the original description, low-normal IgA levels, low to low-normal platelet counts and reduced natural killer (NK)-cell counts also appear as consistent XLN features. However, inverted CD4/CD8 ratios were not found in this family, nor were cases identified with myelodysplastic syndrome or acute myeloid leukaemia. Female carriers exhibited a variable attenuated phenotype. Like L270P WASP, I294T WASP is constitutively active towards actin polymerization. In conclusion, this largest XLN kindred identified to date provides new independent genetic evidence that mutations disrupting the auto-inhibitory GBD of WASP are the cause of XLN. Reduced NK cells, low to low normal platelet counts and low to low-normal IgA levels are also features of XLN.
Beel
Karolien
K
Centre for Human Genetics/Department of Haematology, University Hospital Leuven and University of Leuven, Leuven, Belgium.
Cotter
Melanie M
MM
Blatny
Jan
J
Bond
Jonathan
J
Lucas
Geoff
G
Green
Frances
F
Vanduppen
Vik
V
Leung
Daisy W
DW
Rooney
Sean
S
Smith
Owen P
OP
Rosen
Michael K
MK
Vandenberghe
Peter
P
D000293:Adolescent; D000328:Adult; D002648:Child; D041321:Chromosomes, Human, X; D004252:DNA Mutational Analysis; D005260:Female; D005434:Flow Cytometry; D006579:Heterozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009503:Neutropenia / Q000235:genetics*; D010375:Pedigree; D051316:Wiskott-Aldrich Syndrome Protein / Q000235:genetics*; D049951:X Chromosome Inactivation
C495767:WAS protein, human; D051316:Wiskott-Aldrich Syndrome Protein