title: Avian pathogenic Escherichia coli bind fibronectin and laminin.
abstract: Avian colisepticemia frequently occurs after respiratory tract damage, the primary site for infection allows bacteria to encounter an exposed basement membrane, where laminin and fibronectin are important components. We investigated the ability of an isolate of avian pathogenic Escherichia coli to bind fibronectin and laminin. Using Far-western dot blot analysis, we demonstrated the ability of this microorganism to bind basement membrane proteins fibronectin and laminin. Results from an ELISA-based approach indicate that the binding to these membrane proteins was bacterial-dose dependent. Furthermore, two specific E. coli polypeptides, of 32 kDa and 130 kDa, reacted with laminin and fibronectin, respectively. Further evaluation of these potential bacterial adhesins may provide insights into the pathogenesis of colibacillosis.
authors.affiliation: Departamento de Enfermedades Infecciosas, Unidad Académica de Biología Experimental, Universidad Autónoma de Zacatecas, Calzada de la Revolución Mexicana s/n, Guadalupe Zac, 98600, México.
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Ramírez
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Almanza
authors.forename: Rafael
authors.initials: R
authors.lastname: González
authors.forename: Santos
authors.initials: S
authors.lastname: García
authors.forename: Norma
authors.initials: N
authors.lastname: Heredia
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000378:metabolism* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D005109:Extracellular Matrix / Q000378:metabolism / Q000382:microbiology; D005353:Fibronectins / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism*; D011201:Poultry Diseases / Q000378:metabolism / Q000382:microbiology*
chemical_list: D005353:Fibronectins; D007797:Laminin