Activity of elaeochytrin A from Ferula elaeochytris on leukemia cell lines.
Phytochemical investigation of the roots of Ferula elaeochytris made it possible to isolate two sesquiterpene esters, 6-anthraniloyljaeschkeanadiol (elaeochytrin A) and 4beta-hydroxy-6alpha-(p-hydroxybenzoyloxy)dauc-9-ene (elaeochytrin B), as well as eight known compounds: 6-angeloyljaeschkeanadiol, teferidin, ferutinin, 6-(p-hydroxybenzoyl)epoxyjaeschkeanadiol, 6-(p-hydroxybenzoyl)lancerotriol, 5-caffeoylquinic acid, 1,5-dicaffeoylquinic acid and sandrosaponin IX. The cytotoxic activities of all compounds were investigated on K562R (imatinib-resistant) human chronic myeloid leukaemia and DA1-3b/M2(BCR-ABL) (dasatinib-resistant) mouse leukemia cell line. Elaeochytrin A was the most active compound on both cell lines (IC(50)=12.4 and 7.8microM, respectively). It was also tested on non-resistant human promyelocytic leukemia cells (HL60, IC(50)=13.1microM) and was not toxic to normal peripheral blood mononuclear cells up to 100microM.
Alkhatib
Racha
R
Laboratoire de Pharmacognosie, E.A., Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille 2, Lille, France.
Hennebelle
Thierry
T
Joha
Sami
S
Idziorek
Thierry
T
Preudhomme
Claude
C
Quesnel
Bruno
B
Sahpaz
Sevser
S
Bailleul
François
F
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D005309:Ferula / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000188:drug therapy*; D051379:Mice; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D012717:Sesquiterpenes / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*
D000970:Antineoplastic Agents; D012717:Sesquiterpenes; C534909:elaeochytrin A