Reduction of Escherichia coli O157:H7 in cattle drinking-water by trans-cinnamaldehyde.
Cattle serve as a major reservoir of E. coli O157:H7 and excrete the pathogen in feces. Environmental persistence of E. coli O157:H7 plays a vital role in its epidemiology on farms, and cattle water troughs are a demonstrated long-term reservoir of E. coli O157:H7 for animals. The objective of this study was to investigate the potential of low concentrations of trans-cinnamaldehyde for killing E. coli O157:H7 in cattle drinking-water. A five-strain mixture of E. coli O157:H7 was inoculated (at approximately 8.0 log colony-forming units [CFU]/mL) into 100 mL samples of well water containing 0, 0.03, 0.05, 0.07, or 0.1% trans-cinnamaldehyde. Additionally, water samples containing (1% w/v) bovine feces or feed were also included. The samples were incubated at 21 degrees , 8 degrees , or 4 degrees C for 7 days and tested for viable E. coli O157:H7 on days 0, 1, 3, 5, and 7. Triplicate samples of each treatment and control were included and the study was replicated twice. All concentrations of trans-cinnamaldehyde were effective in killing E. coli O157:H7 in water, but the magnitude of killing significantly increased with increase in trans-cinnamaldehyde concentration and storage temperature (p < 0.05). The presence of feed or feces in water decreased the antibacterial effect of trans-cinnamaldehyde on E. coli O157:H7 (p < 0.05). This study indicated that trans-cinnamaldehyde is effective in killing E. coli O157:H7 in cattle drinking-water, but detailed palatability studies on cattle intake of water containing the antimicrobial are needed.
Charles
Anu Susan
AS
Department of Animal Science, University of Connecticut, Storrs, Connecticut 06269, USA.
Baskaran
Sangeetha Ananda
SA
Murcott
Christine
C
Schreiber
David
D
Hoagland
Thomas
T
Venkitanarayanan
Kumar
K
D000171:Acrolein / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D015169:Colony Count, Microbial; D004197:Disease Reservoirs / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D004326:Drinking; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D005243:Feces / Q000382:microbiology; D005618:Fresh Water / Q000382:microbiology; D013696:Temperature; D013997:Time Factors; D014871:Water Microbiology
D000900:Anti-Bacterial Agents; D000171:Acrolein; C012843:cinnamaldehyde