[Computer karyometry of the thyroid thyrocytes in the conditions of modeling acute radiation injury in animals].
The influence of gamma-radiation on the thyroid gland was studied in the experiments on male rats (4 month old), divided into 6 groups (n = 3). Acute radiation sickness of medullary type was modeled using radial therapeutic unit AGAT-R1 with 60Co radiation source and single total uniform exposure with gamma-quanti at the dose of 7.0 Gy, the dose power--0.78 Gr/min. The first group consisted of intact animals, the second one--the animals, withdrawn out of the experiment 2 days after the exposure with gamma-quanti, the third--after 5 days, the fourth---after 10 days, the fifth--after 15 days and the sixth--after 30 days. Morphometry of the diameter and area of the nucleus of 1500-2000 tyrocytes was made for every period of studying using "VideoTest-Master" program. The samples were ranked with the step of 5.0 microm2, and karyograms were obtained, which had normal distribution in all the cases. In I and II groups the maximal number of the nuclei was noted within the range of 11-15 microm2, as for all the rest period of the experiment, the nuclei of the area of 21-35 microm2 predominated. In the groups III-VI dome-shaped histograms were observed, that is characteristic of follicular and papillary struma maligna. The results obtained show the perspectiveness of using this experimental model combined with computer karyometry for studying the diseases of the neuro-endocrine system, induced by a radiogenic factor.
Ir'ianov
Iu M
IuM
Filimonova
G N
GN
D000208:Acute Disease; D000818:Animals; D002467:Cell Nucleus / Q000473:pathology / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D007090:Image Interpretation, Computer-Assisted; D007620:Karyometry; D008297:Male; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000473:pathology*; D051381:Rats; D013961:Thyroid Gland / Q000473:pathology / Q000528:radiation effects*