Biochemical and structural investigations of Bothropstoxin-II, a myotoxic Asp49 phospholipase A2 from Bothrops jararacussu venom.
Bothropstoxin-II a calcium-dependent enzyme from Bothrops jararacussu venom causes tissue damage and several haemostatic disorders including platelet aggregation. In order to elucidate the structural determinants of its multiple pharmacological activities, we have studied the effects of suramin on Bothropstoxin-II and present details concerning the mode of binding.
Murakami
Mário T
MT
Center for Structural Molecular Biology, Brazilian Synchrotron Light Laboratory, LNLS Campinas, SP, Brazil.
Lourenzoni
Marco R
MR
Arruda
Emerson Z
EZ
Tomaz
Marcelo A
MA
Viçoti
Magno M
MM
Abrego
José R B
JR
Melo
Paulo A
PA
Arni
Raghuvir K
RK
D000465:Algorithms; D000818:Animals; D001665:Binding Sites; D017837:Bothrops; D020134:Catalytic Domain; D003198:Computer Simulation; D003402:Creatine Kinase / Q000378:metabolism; D003435:Crotalid Venoms / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D054501:Group II Phospholipases A2 / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D008958:Models, Molecular; D011487:Protein Conformation; D030162:Reptilian Proteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D053838:Scattering, Small Angle; D013498:Suramin / Q000378:metabolism
D003435:Crotalid Venoms; D030162:Reptilian Proteins; D013498:Suramin; D003402:Creatine Kinase; D054501:Group II Phospholipases A2; C072031:bothropstoxin II