Nidus vespae protein inhibiting proliferation of HepG2 hepatoma cells through extracellular signal-regulated kinase signaling pathways and inducing G1 cell cycle arrest.
A protein named NVP(1) was isolated from Nidus vespae. The aim of the present study was to elucidate whether and how NVP(1) modulates the proliferation of HepG2 cells. NVP(1) at a concentration of 6.6 microg/ml could arrest the cell cycle at stage G1 and inhibit the mRNA expression of cyclinB, cyclinD1 and cyclinE. NVP(1) suppressed cdk2 protein expression, but increased p27 and p21 protein expression. However, NVP(1) did not alter p16 protein expression levels. NVP(1) promoted apoptosis in HepG2 cells as indicated by nuclear chromatin condensation, and in addition, the extracellular signal-regulated kinase (ERK) signaling pathway was activated. Moreover, the p-ERK protein expression level was attenuated when the HepG2 cells were pretreated with ERK inhibitor PD98059. These results demonstrate that NVP(1) inhibits proliferation of HepG2 through ERK signaling pathway. NVP(1) could be a potential drug for liver cancer.
Wang
Changdong
C
China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, the Key Laboratory of Molecular Biophysics of Ministry of Education, College of life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Chen
Peng
P
Jin
Hongjuan
H
Yan
Xu
X
Gan
Lu
L
Li
Yi
Y
Zhou
Shiyi
S
Chang
Junli
J
Wang
Yuesheng
Y
Yang
Guangxiao
G
He
Guangyuan
G
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D016193:G1 Phase / Q000187:drug effects*; D006801:Humans; D019476:Insect Proteins / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D014863:Wasps
D019476:Insect Proteins; D048049:Extracellular Signal-Regulated MAP Kinases