Expression and hormonal regulation of IL-11Ralpha in canine uterus during early pregnancy.
Embryo implantation is critical for the successful establishment of pregnancy. Interleukin-11 (IL-11) is essential for adequate decidualization in the mouse and human via binding to the specific IL-11 receptor alpha (IL-11Ralpha). But the expression and regulation of IL-11 and IL-11Ralpha in the canine endometrium remain unknown. The aim of this study was to investigate the differential expression of IL-11Ralpha in canine uterus during early pregnancy and its regulation under different conditions by in situ hybridization. Interleukin-11Ralpha mRNA was mainly localized in glandular epithelium in canine uterus. There was a low level of IL-11Ralpha expression in the glandular epithelium on days 6, 12 and 17 of pregnancy. On day 20 of pregnancy when embryo implanted, IL-11Ralpha mRNA was highly expressed in the glandular epithelium surrounding the embryo, but not in the luminal epithelium and stroma. On day 23 of pregnancy, the expression of IL-11Ralpha mRNA maintained a constant level compared with the expression of day 20 and increased on day 28 of pregnancy. During the oestrous cycle, a high level of IL-11Ralpha mRNA expression was seen in the oestrous uterus. Progesterone slightly induced the expression of IL-11Ralpha mRNA in the ovariectomized canine uterus. These results suggest that IL-11Ralpha expression is closely related to canine implantation and up-regulated by progesterone.
Guo
B
B
College of Animal Science and Veterinary Medicine, Jilin University, Changchun, P.R. China.
Han
B-C
BC
Tian
Z
Z
Zhao
T-Y
TY
Zeng
L
L
Yang
Z-M
ZM
Yue
Z-P
ZP
D000818:Animals; D004285:Dogs; D010064:Embryo Implantation / Q000502:physiology; D004848:Epithelium / Q000737:chemistry; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D005865:Gestational Age; D017403:In Situ Hybridization; D053703:Interleukin-11 Receptor alpha Subunit / Q000235:genetics*; D010052:Ovariectomy / Q000662:veterinary; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D014599:Uterus / Q000737:chemistry*
D053703:Interleukin-11 Receptor alpha Subunit; D012333:RNA, Messenger; D011374:Progesterone