title: Moricandia arvensis extracts protect against DNA damage, mutagenesis in bacteria system and scavenge the superoxide anion.
abstract: The mutagenic potential of total aqueous, total oligomers flavonoids (TOF), ethyl acetate (EA), chloroform (Chl), petroleum ether (PE) and methanol (MeOH) extracts from aerial parts of Moricandia arvensis was assessed using Ames Salmonella tester strains TA100 and TA1535 with and without metabolic activation (S9), and using plasmid pBluescript DNA assay. None of the different extracts produced a mutagenic effect, except aqueous extract when incubated with Salmonella typhimurium TA100 after metabolic activation. Likewise, the antimutagenicity of the same extracts was tested using the "Ames test". Our results showed that M. arvensis extracts possess antimutagenic effects against sodium azide (SA) in the two tested Salmonella assay systems, except metabolized aqueous and PE extracts when tested with S. typhimurium TA100 assay system. Different extracts were also found to be effective in protecting plasmid DNA against the strand breakage induced by hydroxyl radicals, except PE and aqueous extracts. Antioxidant capacity of the tested extracts was evaluated using the enzymatic (xanthine/xanthine oxidase assay) (X/XOD) and the non enzymatic (NBT/Riboflavine assay) systems. TOF extract was the more effective one in inhibiting both xanthine oxidase activity and NBT reduction.
authors.affiliation: Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Rue Avicenne, Monastir 5000, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Skandrani
authors.forename: Ines
authors.initials: I
authors.lastname: Bouhlel
authors.forename: Ilef
authors.initials: I
authors.lastname: Limem
authors.forename: Jihed
authors.initials: J
authors.lastname: Boubaker
authors.forename: Wissem
authors.initials: W
authors.lastname: Bhouri
authors.forename: Aicha
authors.initials: A
authors.lastname: Neffati
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ben Sghaier
authors.forename: Soumaya
authors.initials: S
authors.lastname: Kilani
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Leila
authors.initials: L
authors.lastname: Ghedira-Chekir
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000145:classification / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology; D019607:Brassicaceae / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000378:metabolism / Q000494:pharmacology; D016488:Medicine, African Traditional; D009152:Mutagenicity Tests; D009153:Mutagens / Q000145:classification / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity*; D051381:Rats; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D019810:Sodium Azide / Q000633:toxicity; D013481:Superoxides / Q000378:metabolism
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D009153:Mutagens; D010936:Plant Extracts; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; D013481:Superoxides; D019810:Sodium Azide