title: Investigation of genetic variants of alpha-1 acid glycoprotein by ultra-performance liquid chromatography-mass spectrometry.
abstract: Genetic variants of human plasma alpha-1 acid glycoprotein (AGP) have been studied in cancer, compared with a group of healthy control. AGP has four genetic variants: AGP F1, F2, and S variants correspond to the ORM1 gene whereas AGP A corresponds to the ORM2 gene. The proportion of ORM1 and ORM2 variants were studied in plasma using a novel UPLC-MS method. Plasma total AGP level was 0.5 +/- 0.2 g L(-1) and the proportions of the ORM1 and ORM2 variants were 76.3 +/- 8.2% and 23.7 +/- 8.2%, respectively. In cancer plasma AGP levels increased fourfold and the proportion of ORM1 variants increased to 88.7 +/- 6.8%. Changes in the proportion of genetic variants due to cancer were clearly significant, as shown by statistical analysis. Three different cancer types have been studied, lymphoma, melanoma, and ovarian cancer. The results did not show any difference depending on cancer type. The results indicate that, in accordance with prior expectations, the ORM1 variant is predominantly responsible for the acute-phase property of AGP.
authors.affiliation: Hungarian Academy of Sciences, Chemical Research Center, 1025, Pusztaszeri 59-67., Budapest, Hungary.
authors.forename: Lívia
authors.initials: L
authors.lastname: Budai
authors.forename: Oliver
authors.initials: O
authors.lastname: Ozohanics
authors.forename: Krisztina
authors.initials: K
authors.lastname: Ludányi
authors.forename: László
authors.initials: L
authors.lastname: Drahos
authors.forename: Tibor
authors.initials: T
authors.lastname: Kremmer
authors.forename: Judit
authors.initials: J
authors.lastname: Krenyacz
authors.forename: Károly
authors.initials: K
authors.lastname: Vékey
mesh_terms: D002851:Chromatography, High Pressure Liquid; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D013058:Mass Spectrometry; D009961:Orosomucoid / Q000032:analysis* / Q000235:genetics*; D015203:Reproducibility of Results
chemical_list: D009961:Orosomucoid