Propofol limits rat myocardial ischemia and reperfusion injury with an associated reduction in apoptotic cell death in vivo.
Propofol, a rapidly acting, short duration, intravenous hypnotic anesthetic induction agent, is often used in clinical situations where myocardial ischemia/ reperfusion (I/R) injury is a threat. The aim of the present study was to evaluate the protective effect of propofol on myocardial I/R injury in rat due to apoptosis. Myocardial I/R injury were induced by occluding the left anterior descending (LAD) coronary artery for 25 min followed by either 2 h or 6 h reperfusion. Apoptosis was evaluated by Western blot analysis (Bcl-2, Bax expression), DNA strand breaks, TUNEL analysis and measuring myocardial caspase-3 activity. Propofol significantly reduced infarct size and improved I/R-induced myocardial contractile dysfunction by improving left ventricular diastolic pressure and positive and negative maximal values of the first derivative (+dp/dt) of left ventricular pressure. Propofol increased Bcl-2/Bax expression ratio and decreased caspase-3 activity in I/R rat hearts, which resulted in reduction of myocardial apoptosis as evidenced by TUNEL analysis and DNA laddering experiments. In an in vitro study, propofol increased H9c2 cell viability against oxidative stress induced by glucose oxidase (GOX) in a dose-dependent manner. These data suggest propofol limits I/R injury with an associated reduction in apoptotic cell death in vivo.
Jin
Yong Chun
YC
Department of Pharmacology School of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.
Kim
Wooyeol
W
Ha
Yu Mi
YM
Shin
Il Woo
IW
Sohn
Ju Tae
JT
Kim
Hye Jung
HJ
Seo
Han Geuk
HG
Lee
Jea Heun
JH
Chang
Ki Churl
KC
D018686:Anesthetics, Intravenous / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000032:analysis; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D020287:In Situ Nick-End Labeling; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D018686:Anesthetics, Intravenous; D006993:Hypnotics and Sedatives; D051028:bcl-2-Associated X Protein; D053148:Caspase 3; D015742:Propofol