Dynamic analysis of on-line high-performance liquid chromatography for multivariate statistical process control.
A continuous process was studied over 83.32 h using on-line high-performance liquid chromatography, involving the acquisition of 252 chromatograms. A method for analysis of these data using multivariate statistical process control on peak tables, in real-time, is described. The normal operating condition (NOC) region of the process was identified using evolving principal components analysis to be between 5.77 and 8.13 h. 19 out of the 37 peaks detected throughout the process were found in the NOC region, the remainder representing undesirable contaminants found elsewhere in the process. A major challenge is to develop the peak table as the process evolves, which is dynamically updated as new peaks are detected after the NOC region: this approach involving an "unlocked" peak table is contrasted to an approach using a "locked" peak table where only peaks detected during the NOC region are included in the model. In addition, results are compared to those obtained using baseline corrected and aligned chromatograms, using a NOC region of 5.85-8.33h. D- and Q-charts were obtained. It is shown that the "unlocked" peak table detects out of control samples best and provides good diagnostic insight into problems with the process.
Kittiwachana
Sila
S
Centre for Chemometrics, School of Chemistry, University of Bristol, Cantocks Close, Bristol BS8 1TS, UK.
Ferreira
Diana L S
DL
Fido
Louise A
LA
Thompson
Duncan R
DR
Escott
Richard E A
RE
Brereton
Richard G
RG
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015999:Multivariate Analysis; D009862:Online Systems; D025341:Principal Component Analysis; D011786:Quality Control