Isolation and characterization of an atypical LEA protein coding cDNA and its promoter from drought-tolerant plant Prosopis juliflora.
Plant growth and productivity are adversely affected by various abiotic and biotic stress factors. Despite the wealth of information on abiotic stress and stress tolerance in plants, many aspects still remain unclear. Prosopis juliflora is a hardy plant reported to be tolerant to drought, salinity, extremes of soil pH, and heavy metal stress. In this paper, we report the isolation and characterization of the complementary DNA clone for an atypical late embryogenesis abundant (LEA) protein (Pj LEA3) and its putative promoter sequence from P. juliflora. Unlike typical LEA proteins, rich in glycine, Pj LEA3 has alanine as the most abundant amino acid followed by serine and shows an average negative hydropathy. Pj LEA3 is significantly different from other LEA proteins in the NCBI database and shows high similarity to indole-3 acetic-acid-induced protein ARG2 from Vigna radiata. Northern analysis for Pj LEA3 in P. juliflora leaves under 90 mM H2O2 stress revealed up-regulation of transcript at 24 and 48 h. A 1.5-kb fragment upstream the 5' UTR of this gene (putative promoter) was isolated and analyzed in silico. The possible reasons for changes in gene expression during stress in relation to the host plant's stress tolerance mechanisms are discussed.
George
Suja
S
M.S. Swaminathan Research Foundation, Taramani, Chennai 600113, India.
Usha
B
B
Parida
Ajay
A
D020121:5' Untranslated Regions / Q000235:genetics; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics*; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification*; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D029862:Prosopis / Q000187:drug effects / Q000235:genetics*; D016415:Sequence Alignment
D020121:5' Untranslated Regions; D018076:DNA, Complementary; D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant; D006861:Hydrogen Peroxide