title: Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies.
abstract: BACKGROUND
  Autoantibodies to the ribosomal P proteins represent a highly specific marker for the diagnosis of systemic lupus erythematosus, where they have been associated with certain clinical manifestations. Historically, autoantibodies against ribosomal P proteins have been detected by indirect immunofluorescence, immunodiffusion, immunoblot, and other immunoassays. More recently, enzyme-linked immunosorbent assays and line and addressable laser bead immunoassays have become more widely used. The primary goal of this study was to determine the sensitivity of indirect immunofluorescence using conventional HEp-2 substrates in the detection of sera with ribosomal P antibodies as detected by other immunoassays.
  
  
  METHODS
  Anti-ribosomal P-positive sera (n = 345) as detected by an addressable laser bead immunoassay were collected between 2003 and 2007 and analysed by indirect immunofluorescence. Furthermore, 51 anti-ribosomal P-positive samples from an unselected systemic lupus erythematosus cohort (n = 100) and the Centers for Disease Control and Prevention (CDC) anti-nuclear antibody (ANA) reference sera were tested for anti-ribosomal P reactivity.
  
  
  RESULTS
  In the cohort of 345 anti-ribosomal P-positive samples identified by addressable laser bead immunoassay, a low sensitivity (<30%) of indirect immunofluorescence on HEp-2 cell substrates was observed. Although the degree of sensitivity varied among different manufacturers, all immunofluorescence substrates exhibited limited sensitivity and false-negative results were not restricted to samples with low anti-ribosomal P titers. Even the anti-ribosomal P reactivity of CDC ANA reference serum number 12 was not clearly predictable by indirect immunofluorescence. Comparison of five different methods for the detection of anti-ribosomal P found moderate qualitative agreements.
  
  
  CONCLUSIONS
  Based on our data, we conclude that indirect immunofluorescence on HEp-2 cells is not a reliable screening test for the prediction of ribosomal P antibodies. As this method is widely used as a first-line screening test for anti-nuclear and other autoantibodies, special considerations for the detection of ribosomal P antibodies are needed. As with many other autoantibodies, further effort is required for the standardisation of ribosomal P immunoassays.
authors.affiliation: Dr Fooke Laboratorien GmbH, Neuss, Germany. m.mahler.job@web.de
authors.forename: Michael
authors.initials: M
authors.lastname: Mahler
authors.forename: Jennifer T
authors.initials: JT
authors.lastname: Ngo
authors.forename: Johannes
authors.initials: J
authors.lastname: Schulte-Pelkum
authors.forename: Tanja
authors.initials: T
authors.lastname: Luettich
authors.forename: Marvin J
authors.initials: MJ
authors.lastname: Fritzler
mesh_terms: D001323:Autoantibodies / Q000032:analysis* / Q000097:blood; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000592:standards; D006367:HeLa Cells; D006801:Humans; D012269:Ribosomal Proteins / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D012269:Ribosomal Proteins