title: Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies.
abstract: The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, 155 Li-Nong Street, Section 2, Taipei 11221, Taiwan.
authors.forename: Han-Wen
authors.initials: HW
authors.lastname: Guo
authors.forename: Chien-Tsun
authors.initials: CT
authors.lastname: Chen
authors.forename: Yau-Huei
authors.initials: YH
authors.lastname: Wei
authors.forename: Oscar K
authors.initials: OK
authors.lastname: Lee
authors.forename: Vladimir
authors.initials: V
authors.lastname: Gukassyan
authors.forename: Fu-Jen
authors.initials: FJ
authors.lastname: Kao
authors.forename: Hsing-Wen
authors.initials: HW
authors.lastname: Wang
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009243:NAD / Q000032:analysis*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D009243:NAD