Effect of low-level laser therapy after implantation of poly-L-lactic/polyglycolic acid in the femurs of rats.
This study evaluated the use of red and infrared lasers on tissue surrounding the femurs of 60 rats randomly divided into three groups after implantation of bioabsorbable plates. The control group were not subjected to laser irradiation; group A was treated with red laser [indium-gallium-aluminum-phosphide (InGaAlP) laser, wavelength 685 nm, 35 mW, continuous wave (CW), Ø = 0.06 cm, 2.23 min], and group B was subjected to infrared laser [gallium-aluminum-arsenium (GaAlAs) laser, wavelength 830 nm, 50 mw, CW, Ø = 0.06 cm, 1.41 min], both at 10 J/cm(2). Samples were stained with hematoxylin and eosin (H&E) and examined microscopically. Results showed that the laser irradiation had had a positive photobiomodulation effect on inflammation, confirmed by a better histologic pattern than that of the control group at 3 days and 7 days. Semiquantitative analysis revealed that groups A and B had a histologic score significantly greater than that of the control group at 3 days. At 21 days, histomorphometric analysis revealed a more intense inflammation in the red laser group than in the other groups. We concluded that low-level laser therapy (LLLT) has positive effects on the photobiomodulation of inflammation in the tissues surrounding the poly-L-lactic/polyglycolic acid (PLLA/PGA) bioabsorbable plate. It stimulated vascularization, fibroblast proliferation, and collagen deposition.
Freddo
Angelo Luiz
AL
School of Dentistry, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil.
Rodrigo
Simone Marja
SM
Massotti
Fabrício Poletto
FP
Etges
Adriana
A
de Oliveira
Marília Gerhardt
MG
D000818:Animals; D001672:Biocompatible Materials; D001860:Bone Plates; D001861:Bone Regeneration / Q000528:radiation effects; D005269:Femur / Q000473:pathology / Q000528:radiation effects / Q000601:surgery; D007249:Inflammation / Q000473:pathology / Q000517:prevention & control / Q000532:radiotherapy; D019344:Lactic Acid; D028022:Low-Level Light Therapy; D008297:Male; D008422:Materials Testing; D016348:Osseointegration / Q000528:radiation effects; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid