title: Structure and dynamics of single DNA molecules manipulated by magnetic tweezers and or flow.
abstract: Here we describe the use of magnetic tweezers and or microfluidics to manipulate single DNA molecules. We describe experiment which employ magnetic tweezers coupled to an inverted microscope as well as the use of a magnetic tweezers setup with an upright microscope. Using a chamber prepared via soft lithography, we also describe a microfluidic device for the manipulation of individual DNA molecules. Finally, we present some past successful examples of using these approaches to elucidate unique information about protein-nucleic acid interactions.
authors.affiliation: Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
authors.forename: Sanford H
authors.initials: SH
authors.lastname: Leuba
authors.forename: Travis B
authors.initials: TB
authors.lastname: Wheeler
authors.forename: Chao-Min
authors.initials: CM
authors.lastname: Cheng
authors.forename: Philip R
authors.initials: PR
authors.lastname: LeDuc
authors.forename: Mónica
authors.initials: M
authors.lastname: Fernández-Sierra
authors.forename: Edwin
authors.initials: E
authors.lastname: Quiñones
mesh_terms: D042002:Chromatin Assembly and Disassembly; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000737:chemistry; D008280:Magnetics / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods; D009690:Nucleic Acid Conformation; D014158:Transcription, Genetic
chemical_list: D004247:DNA; D005090:Exodeoxyribonucleases