Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin.
The ratio of hapten and bovine serum albumin in an antigenconjugate was determined by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Ahybridoma secreting monoclonal antibody against ginsenosideRg(1) was produced by fusing sprenocytes immunized with aginsenoside Rg(1)-bovine serum albumin conjugate withHAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very smallcross-reaction appeared with ginsenoside Re. The full measuringrange of the assay extends from 0.3 mug ml(-1) to 10 mug ml(-1) ofginsenoside Rg(1).
Fukuda
N
N
Department of Pharmacognosy, Graduate school of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higahsi-ku, Fukuoka, 812,-8582, Japan.
Tanaka
H
H
Shoyama
Y
Y