Selective, peroxidase substrate based "signal-on" colorimetric assay for the detection of chromium (VI).
Due to the potentially adverse effects of the chromium (VI) on the human health and also on the environment, the quantitative determination of Cr(VI) is of particular interest. This work herein reports a facile, selective and rapid colorimetric determination of Cr(VI) based on the peroxidase substrate-2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) as the color developing agent. ABTS, which was usually acted as peroxidase substrate for the enzyme linked immunosorbent assay, is used here for the first time to fabricate the "signal-on" colorimetric Assay for Cr(VI). The ABTS was chosen instead of the commonly used 1,5-diphenylcarbazide (DPC) due to its good solubility, stability, sensitivity and low background. This method provided a convenient colorimetric detection of Cr(VI) with a wider linear range from 8.33 microg L(-1) to 1.25 mg L(-1) by recording the absorption spectra at the wavelength of 419 nm and a low detection limit of 7.87 microg L(-1). In addition, the entire detection takes less than 10 min.
Li
Jing
J
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Changchun, Jilin, China.
Wei
Hui
H
Guo
Shaojun
S
Wang
Erkang
E
D052160:Benzothiazoles / Q000737:chemistry / Q000378:metabolism*; D002857:Chromium / Q000032:analysis*; D003124:Colorimetry / Q000379:methods*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism; D012680:Sensitivity and Specificity; D013451:Sulfonic Acids / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
D052160:Benzothiazoles; D013451:Sulfonic Acids; D002857:Chromium; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D010544:Peroxidases