title: Biochemical and structural investigations of Bothropstoxin-II, a myotoxic Asp49 phospholipase A2 from Bothrops jararacussu venom.
abstract: Bothropstoxin-II a calcium-dependent enzyme from Bothrops jararacussu venom causes tissue damage and several haemostatic disorders including platelet aggregation. In order to elucidate the structural determinants of its multiple pharmacological activities, we have studied the effects of suramin on Bothropstoxin-II and present details concerning the mode of binding.
authors.affiliation: Center for Structural Molecular Biology, Brazilian Synchrotron Light Laboratory, LNLS Campinas, SP, Brazil.
authors.forename: Mário T
authors.initials: MT
authors.lastname: Murakami
authors.forename: Marco R
authors.initials: MR
authors.lastname: Lourenzoni
authors.forename: Emerson Z
authors.initials: EZ
authors.lastname: Arruda
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Tomaz
authors.forename: Magno M
authors.initials: MM
authors.lastname: Viçoti
authors.forename: José R B
authors.initials: JR
authors.lastname: Abrego
authors.forename: Paulo A
authors.initials: PA
authors.lastname: Melo
authors.forename: Raghuvir K
authors.initials: RK
authors.lastname: Arni
mesh_terms: D000465:Algorithms; D000818:Animals; D001665:Binding Sites; D017837:Bothrops; D020134:Catalytic Domain; D003198:Computer Simulation; D003402:Creatine Kinase / Q000378:metabolism; D003435:Crotalid Venoms / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D054501:Group II Phospholipases A2 / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D008958:Models, Molecular; D011487:Protein Conformation; D030162:Reptilian Proteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D053838:Scattering, Small Angle; D013498:Suramin / Q000378:metabolism
chemical_list: D003435:Crotalid Venoms; D030162:Reptilian Proteins; D013498:Suramin; D003402:Creatine Kinase; D054501:Group II Phospholipases A2; C072031:bothropstoxin II