Effect of HCG in the presence of HCG antibodies on the follicle, hormone concentrations, and oocyte in mares.
Follicle blood flow, follicular-fluid and plasma hormone concentrations, and oocyte quality were studied 30 h after an ovulation-inducing hCG treatment when the pre-ovulatory follicle was 32 mm. Mares were grouped as positive (n = 16) and negative (n = 44) for hCG antibodies before the experimental hCG treatment. Percentage of the follicle wall with blood flow signals was less (p < 0.05) in the antibody positive group than in the negative group. The concentrations of follicular-fluid oestradiol and free IGF1, and plasma oestradiol were greater (p < 0.001), and follicular-fluid progesterone (p < 0.001) and plasma LH (p < 0.02) were less in the antibody-positive group than in the negative group. For recovered oocytes at 30 h (n = 37), the antibody-positive group had fewer (p < 0.001) mature (MII) oocytes than the antibody-negative group. Results were attributable to highly effective neutralization of the hCG in the antibody-positive group.
Siddiqui
M A R
MA
Eutheria Foundation, Cross Plains, WI 53528, USA.
Gastal
E L
EL
Gastal
M O
MO
Beg
M A
MA
Ginther
O J
OJ
D000818:Animals; D000906:Antibodies / Q000097:blood*; D002452:Cell Count; D006063:Chorionic Gonadotropin / Q000008:administration & dosage* / Q000097:blood / Q000276:immunology*; D004958:Estradiol / Q000032:analysis / Q000097:blood; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry; D006736:Horses / Q000276:immunology* / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007986:Luteinizing Hormone / Q000097:blood; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D006080:Ovarian Follicle / Q000098:blood supply / Q000000981:diagnostic imaging / Q000187:drug effects*; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary; D011374:Progesterone / Q000032:analysis; D014463:Ultrasonography
D000906:Antibodies; D006063:Chorionic Gonadotropin; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D007986:Luteinizing Hormone