Determination of conventional protein kinase C isoforms involved in high intraocular pressure-induced retinal ischemic preconditioning of rats.
Evidence indicates that conventional protein kinase C (cPKC) plays a pivotal role in the development of retinal ischemic preconditioning (IPC). In this study, the effect of high intraocular pressure (IOP)-induced retinal IPC on cPKC isoform-specific membrane translocation and protein expression were observed. We found that cPKCgamma membrane translocation increased significantly at the early stage (20min-1h), while the protein expression levels of cPKCalpha and gamma were markedly elevated in the delayed retinal IPC (12-168h) of rats. The increased protein expressions of cPKCalpha at 72h and cPKCgamma at 24h after IPC were further confirmed by immunofluorescence staining. In addition, we found that cPKCgamma co-localized with retinal ganglion cell (RGC)-specific marker, neurofilaments heavy chain (NF-H) by using double immunofluorescence labeling. These results suggest that increased cPKCgamma membrane translocation and up-regulated protein expressions of cPKCalpha and gamma are involved in the development of high IOP-induced rat retinal IPC.
Ding
Jingwen
J
Beijing Tongren Eye Center, Capital Medical University Affiliated Beijing Tongren Hospital, Vision Science Laboratory, School of Ophthalmology, Beijing, China.
Ding
Ning
N
Wang
Ningli
N
Lu
Qingjun
Q
Lu
Ningning
N
Yang
Diya
D
Bu
Xiangning
X
Han
Song
S
Li
Junfa
J
D000818:Animals; D007511:Ischemia / Q000201:enzymology / Q000517:prevention & control; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D009798:Ocular Hypertension / Q000201:enzymology / Q000503:physiopathology*; D020033:Protein Isoforms / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000201:enzymology; D012171:Retinal Vessels / Q000503:physiopathology*; D015854:Up-Regulation
D020033:Protein Isoforms; D011493:Protein Kinase C