[Molecular genetic method of rapid detection of Bordetella pertussis strains are possessed of different ptx genes].
Features of structure of different B. pertussis genes are studied in many countries of the world, and, first of all, ptxA gene, which encodes main protective antigen of the microbe--pertussis toxin. Starting from 1980s, B. pertussis strains with new "non-vaccine" allele ptxA1 gradually displaced strains with old "vaccine" alleles--ptxA2 and ptxA4, and now the formers dominate in circulating bacterial population. Molecular genetic method of rapid identification of B. pertussis strains, based on the differences in ptxA gene structure, was developed. The method using phenomenon of endonuclease restriction can be applied for differentiation of B. pertussis from B. parapertussis strains in diagnostic purposes.
Borisova
O Iu
OIu
Kombarova
S Iu
SIu
Gadua
N T
NT
Mazurova
I K
IK
D000483:Alleles; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D005798:Genes, Bacterial; D006801:Humans; D037342:Pertussis Toxin / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D014917:Whooping Cough / Q000175:diagnosis*
D004269:DNA, Bacterial; D037342:Pertussis Toxin; D015252:Deoxyribonucleases, Type II Site-Specific; C046719:GGCGCC-specific type II deoxyribonucleases