title: Elongated mouse chromosomes suitable for enhanced molecular cytogenetics.
abstract: Characterization of genetic disorders in humans and animal models requires identification of chromosomal aberrations. However, identifying fine deletions or insertion in metaphase chromosomes has been always a challenge due to limitations of resolution. In this study we developed a rapid method for chromosome elongation using two different intercalating agents: ethidium bromide and 5-bromo-2'-deoxyuridine (BrdU), together with a short-term mitotic block using colcemid. About 70% of the chromosomes from cells that underwent this elongation procedure reached three times longer than those prepared from control cells. FISH experiments using elongated chromosomes revealed a duplicated region of chromosome 11 that was not visible in cells prepared with conventional methods.
authors.affiliation: Manitoba Institute of Cell Biology, Cancer Care Manitoba, University of Manitoba, 675 McDermot Avenue, Winnipeg, MB, R3E 0V9, Canada.
authors.forename: Sherif
authors.initials: S
authors.lastname: Louis
authors.forename: Katalin
authors.initials: K
authors.lastname: Benedek
authors.forename: Michael
authors.initials: M
authors.lastname: Mowat
authors.forename: George
authors.initials: G
authors.lastname: Klein
authors.forename: Sabine
authors.initials: S
authors.lastname: Mai