title: Protective effects of plicatin B on micronucleus induction in cultured human lymphocytes by different mutagens.
abstract: Among flavonoids, chalcones have been identified as interesting compounds having antioxidant, antimutagenic and antitumour properties. In this study we have evaluated the clastogenicity of plicatin B on human lymphocytes (whole blood and isolated lymphocytes) and its anticlastogenic activity, in the same cellular systems, using mitomycin C (MMC), radio-mimetic bleomycin (BL) and hydrogen peroxide (H2O2) as reference clastogens. Plicatin B per se resulted cytotoxic at high dose but non-clastogenic. The clastogenic effects induced by MMC and BL were significantly reduced (-33%) whereas with H2O2 the protective effect of plicatin B was observed (-62%) only at the greater H2O2 dose. The anticlastogen effects of plicatin B were effective when the chalcone was introduced in the culture, 1 h before the reference clastogens.
authors.affiliation: Biochemistry and Mutagenesis in Somatic Cells Unit, IBF-CNR, Via Moruzzi No. 1, 56124 Pisa, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Eventi
authors.forename: Valter
authors.initials: V
authors.lastname: Lubrano
authors.forename: Roberto
authors.initials: R
authors.lastname: Scarpato
authors.forename: Gino
authors.initials: G
authors.lastname: Turchi
mesh_terms: D000179:Acrylates / Q000737:chemistry / Q000494:pharmacology*; D000328:Adult; D001761:Bleomycin; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D016685:Mitomycin; D015394:Molecular Structure; D009153:Mutagens / Q000633:toxicity*
chemical_list: D000179:Acrylates; D009153:Mutagens; D001761:Bleomycin; D016685:Mitomycin; C098495:plicatin B acrylate; D006861:Hydrogen Peroxide