Genistein inhibits the development of atherosclerosis via inhibiting NF-kappaB and VCAM-1 expression in LDLR knockout mice.
Diet can be an important factor that influences risks for cardiovascular disease. Genistein (4',5,7-trihydroxyisoflavone), rich in soy, is one candidate that may benefit the cardiovascular system. Here, we explored the effect of genistein in atherosclerosis (AS) development in an in vivo mouse model. Low-density lipoprotein receptor (LDLR) knockout mice were allocated to control, model, and genistein groups. Our results showed that genistein significantly reduced the formation and development of atherosclerotic plaques ((4.68 +/- 1.18) x106 versus (6.65 +/- 1.51) x106 microm2, p < 0.05). In the genistein group, compared with the model group, total antioxidant capacity (TAC) level was 85.5 +/- 15.6 versus 203.4 +/- 32.6 mmol/L (p < 0.01); malondialdehyde (MDA) level was 3.79 +/- 0.28 versus 3.06 +/- 0.31 mmol/L (p < 0.01), and superoxide dismutase (SOD) activity was 86.1 +/- 6.1 versus 139.1 +/- 25.1 U/mL (p < 0.01). Therefore, genistein was able to enhance serum antioxidative ability in our mouse model. Genistein had no influence, however, on serum cholesterol and lipid profiles. Genistein also markedly downregulated the expression of nuclear factor (NF)-kappaB and vascular cell adhesion molecule (VCAM)-1 in aortas of mice (p < 0.05). These observations suggest that genistein may inhibit AS in LDLR-/- mice via enhancing serum antioxidation and downregulating NF-kappaB and VCAM-1 expression in the aorta.
Wang
Juejin
J
Department of Pharmacology, Nanjing Medical University, Hanzhong Road 140, Nanjing 210029, China.
Zhang
Rongjian
R
Xu
Youhua
Y
Zhou
Hong
H
Wang
Bin
B
Li
Shengnan
S
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D050197:Atherosclerosis / Q000473:pathology / Q000517:prevention & control*; D001835:Body Weight / Q000187:drug effects; D002316:Cardiotonic Agents; D002784:Cholesterol / Q000097:blood; D018450:Disease Progression; D004435:Eating / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D007150:Immunohistochemistry; D008055:Lipids / Q000097:blood; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D011973:Receptors, LDL / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013481:Superoxides / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
D000975:Antioxidants; D002316:Cardiotonic Agents; D008055:Lipids; D016328:NF-kappa B; D011973:Receptors, LDL; D019010:Vascular Cell Adhesion Molecule-1; D013481:Superoxides; D008315:Malondialdehyde; D002784:Cholesterol; D019833:Genistein