title: Laboratory maintenance of Rickettsia rickettsii.
abstract: This unit includes protocols for the laboratory maintenance of the obligate intracellular bacterium Rickettsia rickettsii, including propagation in mammalian cell cultures, as well as isolation, counting, and storage procedures. Regulations for working with R. rickettsii in biosafety level 3 containment are also discussed.
authors.affiliation: Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Ammerman
authors.forename: Magda
authors.initials: M
authors.lastname: Beier-Sexton
authors.forename: Abdu F
authors.initials: AF
authors.lastname: Azad
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002460:Cell Line; D015169:Colony Count, Microbial / Q000379:methods; D006801:Humans; D011309:Preservation, Biological / Q000379:methods; D012284:Rickettsia rickettsii / Q000254:growth & development* / Q000302:isolation & purification