Interactive effects of elevated CO2 and ozone on leaf thermotolerance in field-grown Glycine max.
Humans are increasing atmospheric CO2, ground-level ozone (O3), and mean and acute high temperatures. Laboratory studies show that elevated CO2 can increase thermotolerance of photosynthesis in C3 plants. O3-related oxidative stress may offset benefits of elevated CO2 during heat-waves. We determined effects of elevated CO2 and O3 on leaf thermotolerance of field-grown Glycine max (soybean, C3). Photosynthetic electron transport (et) was measured in attached leaves heated in situ and detached leaves heated under ambient CO2 and O3. Heating decreased et, which O3 exacerbated. Elevated CO2 prevented O3-related decreases during heating, but only increased et under ambient O3 in the field. Heating decreased chlorophyll and carotenoids, especially under elevated CO2. Neither CO2 nor O3 affected heat-shock proteins. Heating increased catalase (except in high O3) and Cu/Zn-superoxide dismutase (SOD), but not Mn-SOD; CO2 and O3 decreased catalase but neither SOD. Soluble carbohydrates were unaffected by heating, but increased in elevated CO2. Thus, protection of photosynthesis during heat stress by elevated CO2 occurs in field-grown soybean under ambient O3, as in the lab, and high CO2 limits heat damage under elevated O3, but this protection is likely from decreased photorespiration and stomatal conductance rather than production of heat-stress adaptations.
Mishra
Sasmita
S
Department of Environmental Sciences, University of Toledo, Toledo, Ohio, USA. sasmita.mishra@utoledo.edu
Heckathorn
Scott A
SA
Barua
Deepak
D
Wang
Dan
D
Joshi
Puneet
P
Hamilton Iii
E William
EW
Frantz
Jonathan
J
D002245:Carbon Dioxide / Q000633:toxicity*; D002338:Carotenoids / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D010126:Ozone / Q000633:toxicity*; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects* / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
D010940:Plant Proteins; D002734:Chlorophyll; D002245:Carbon Dioxide; D002338:Carotenoids; D010126:Ozone; D002374:Catalase; D013482:Superoxide Dismutase