Cellular receptors and HCV entry.
After attachment to specific receptors on the surfaces of target cells, hepatitis C virus (HCV) particles are thought to be internalized to endosomes, where low pH induces fusion between the viral and cellular membranes, delivering the HCV genome into the cytoplasm. Here, we describe methods to study the early events in HCV infection; the interactions with cellular receptors and the mechanism of entry.
Flint
Mike
M
Wyeth Antiviral Research, Pearl River, NY, USA.
Tscherne
Donna M
DM
D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D004705:Endocytosis / Q000187:drug effects; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005110:Extracellular Space / Q000378:metabolism; D016174:Hepacivirus / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011991:Receptors, Virus / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000276:immunology; D060225:Tetraspanin 28; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D053586:Virus Internalization / Q000187:drug effects
D015703:Antigens, CD; D000998:Antiviral Agents; C554798:CD81 protein, human; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D060225:Tetraspanin 28; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus