title: Expression of chlorophyllase is not induced during autumnal yellowing in Ginkgo biloba.
abstract: Autumnal tints are one of the most fascinating natural phenomena, but the molecular mechanism of chlorophyll (Chl-)degradation in deciduous trees has not been fully understood. In this study, from the leaves of Ginkgo biloba, chlorophyllase-homologous GbCLH was cloned by RT-PCR with degenerated primers. The expression of GbCLH in different yellowing stages was analyzed by Northern hybridization. The expression level of GbCLH was highest in green leaves and significantly declined during the process of leaf yellowing. These results suggested that GbCLH should be involved in chlorophyll-homeostasis in Ginkgo biloba.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
authors.forename: Lei
authors.initials: L
authors.lastname: Tang
authors.forename: Atsushi
authors.initials: A
authors.lastname: Okazawa
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Itoh
authors.forename: Ei-ichiro
authors.initials: E
authors.lastname: Fukusaki
authors.forename: Akio
authors.initials: A
authors.lastname: Kobayashi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology; D020441:Ginkgo biloba / Q000201:enzymology* / Q000502:physiology; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000201:enzymology / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D011994:Recombinant Proteins; D002734:Chlorophyll; D002265:Carboxylic Ester Hydrolases; C019875:chlorophyllase