title: Synthesis of benzofurazan derivatization reagents for short chain carboxylic acids in liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS).
abstract: Benzofurazan derivatization reagents, 4-[2-(N,N-dimethylamino)ethylaminosulfonyl]-7-(2-aminopentylamino)-2,1,3-benzoxadiazole (DAABD-AP) and 4-[2-(N,N-dimethylamino) ethylaminosulfonyl]-7-(2-aminobutylamino)-2,1,3-benzoxadiazole (DAABD-AB), for short-chain carboxylic acids in liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) were synthesized. These reagents reacted with short chain carboxylic acids in the presence of the condensation reagents at 60 degrees C for 60 min. The generated derivatives were separated on the reversed-phase column and detected by ESI-MS/MS with the detection limits of 0.1-0.12 pmol on column. Upon collision-induced dissociation, a single and intense product ion at m/z 151 was observed. These results indicated that DAABD-AP and DAABD-AB are suitable as the derivatization reagents in LC/ESI-MS/MS analysis.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. santa@mol.f.u-tokyo.ac.jp
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Santa
authors.forename: Osama Y
authors.initials: OY
authors.lastname: Al-Dirbashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshikado
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fukushima
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Imai
mesh_terms: D001583:Benzoxazoles / Q000737:chemistry*; D002264:Carboxylic Acids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D010069:Oxadiazoles / Q000138:chemical synthesis*; D021241:Spectrometry, Mass, Electrospray Ionization; D013449:Sulfonamides / Q000138:chemical synthesis*; D053719:Tandem Mass Spectrometry
chemical_list: C507985:4-(2-(N,N-dimethylamino)ethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole; D001583:Benzoxazoles; D002264:Carboxylic Acids; D010069:Oxadiazoles; D013449:Sulfonamides; C020160:benzofurazan