Alcohols which have been in contact with any plastics may interfere in radioimmunoassays of progesterone.
In recent years there has been increasing use of plastic rather than glass containers for many liquids, including wine. However we have found that residue from commercially obtained 'pure' ethanol dispensed in plastic bottles interferes in some biochemical assays. We have observed a volume-dependent decrease in maximally bound ligand in radioimmunoassays of progesterone. The resulting shift in the standard curve leads to an underestimation of the analyte concentrations and to altered estimation of cross reactivity by competing ligands. These effects became apparent in assays with high sensitivity (500 pg or less). All sources of ethanol obtainable in Quebec contained impurities. A similar effect was also produced by 'pure' methanol. The reduction in maximally bound ligand was amplified when the alcohol was aliquoted using plastic pipette tips. We conclude that alcohols which have had any contact with plastics are not safe to use in immunoassays of progesterone (or its metabolites as estimated according to cross-reactivity after HPLC) and may affect other assays. If the use of alcohol and plastic tips cannot be avoided, the amount of alcohol used should be reduced to 1% or less. This can be accomplished by preparing steroid standards in assay buffers containing albumin or gelatin, which enhance the solubility of steroids in aqueous media.
Ocvirk
Rok
R
Department of Experimental Medicine, McGill University, 1110 Pine Avenue West, Montreal, Quebec, Canada H3A 1A1; Department of Psychiatry, McGill University, 1033 Pine Avenue West, Montreal, Quebec, Canada H3A 1A1.
Bisson
Jennifer M
JM
Murphy
Beverley E Pearson
BE
D016477:Artifacts; D000431:Ethanol / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D000432:Methanol / Q000737:chemistry*; D010969:Plastics / Q000737:chemistry*; D011374:Progesterone / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D012015:Reference Standards; D014835:Volatilization
D010969:Plastics; D000431:Ethanol; D011374:Progesterone; D000432:Methanol