Resident enteric microbiota and CD8+ T cells shape the abundance of marginal zone B cells.
Since enteric microbial composition is a distinctive and stable individual trait, microbial heterogeneity may confer lifelong, non-genetic differences between individuals. Here we report that C57BL/6 mice bearing restricted flora microbiota, a distinct but diverse resident enteric microbial community, are numerically and functionally deficient in marginal zone (MZ) B cells. Surprisingly, MZ B-cell levels are minimally affected by germ-free conditions or null mutations of various TLR signaling molecules. In contrast, MZ B-cell depletion is exquisitely dependent on cytolytic CD8(+) T cells, and includes targeting of a cross-reactive microbial/endogenous MHC class 1B antigen. Thus, members of certain enteric microbial communities link with CD8(+) T cells as a previously unappreciated mechanism that shapes innate immunity dependent on innate-like B cells.
Wei
Bo
B
Department of Pathology and Laboratory Medicine, UCLA David Geffen School of Medicine, Los Angeles, CA 90095-1732, USA.
Su
Thomas T
TT
Dalwadi
Harnisha
H
Stephan
Robert P
RP
Fujiwara
Daisuke
D
Huang
Tiffany T
TT
Brewer
Sarah
S
Chen
Lindy
L
Arditi
Moshe
M
Borneman
James
J
Rawlings
David J
DJ
Braun
Jonathan
J
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000382:microbiology*; D002454:Cell Differentiation / Q000276:immunology; D002465:Cell Movement; D048430:Cell Shape; D051379:Mice; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology; D051193:Toll-Like Receptors / Q000276:immunology
D011506:Proteins; D051193:Toll-Like Receptors; C043309:proliferation regulatory factors, human urine