[Initial study on the discrimination of oral microorganisms with a metabonomics method].
OBJECTIVE
To establish the spectra of metabolites that coued be employed in identification of oral pathogenic bacteria, and try to find a convenient and rapid way to discriminate oral microorganisms.


METHODS
Suspensions of Streptococcus mutans ATCC 25175, Streptococcus sanguis ATCC 10556 and Lactobacillus acidophilus ATCC 4356 with same density were preparecd and cultured respectively at improved TPY liquid culture medium. The growth quantity were measured periodically by a turbidimeter. And the growth curves of the inoculated bacteria were completed. The culture solutions in stationary phase of the three bacteria were tested with 1H-nuclear magnetic resonance (1H-NMR) spectroscopy respectively. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis (PCA).


RESULTS
The PCA showed the obvious clustering phenomena and the points of three group differentially centralized to three clusters. Therefore, the NMR-based metabonomics profiles could discriminate the three different kinds of bacteria.


CONCLUSIONS
The metabonomics is a promising new technology for developing to a rapid discrimination method of oral pathogenic bacteria.
Xiong
Ping
P
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Zhou
Jing-lin
JL
Xiao
Li-ying
LY
Kong
Xiang-li
XL
Li
Ji-yao
JY
Jia
Xiang-ming
XM
Li
Wei
W
D001419:Bacteria; D003470:Culture Media; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D013295:Streptococcus mutans
D003470:Culture Media