title: Isolation and confirmation of Yersinia pestis mutants exempt from select agent regulations.
abstract: This unit describes protocols for Yersinia pestis to confirm plasmid profiles, construct and confirm a Deltapgm mutation, and cure the low-calcium response (Lcr) plasmid encoding a type III secretion system (TTSS). Strains lacking either the chromosomal pgm locus or the Lcr plasmid can be safely studied under BSL-2 conditions and are exempt from Select Agent regulations in the U.S.
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky, USA.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Perry
authors.forename: Scott W
authors.initials: SW
authors.lastname: Bearden
mesh_terms: D003264:Containment of Biohazards / Q000379:methods; D017353:Gene Deletion; D010957:Plasmids; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*; D015010:Yersinia pestis / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*
chemical_list: D037521:Virulence Factors