Flavan-3-ols from Ulmus davidiana var. japonica with inhibitory activity on protein glycation.
Four flavan-3-ols, (+)-catechin ( 1), (+)-catechin 7- O- beta- D-apiofuranoside ( 2), (+)-catechin 7- O- beta- D-xylopyranoside ( 3), (+)-catechin 7- O- beta- D-glucopyranoside ( 4), and proanthocyanidin A-1 ( 5) as well as three other constituents, isolated from an EtOAc-soluble extract of the stem barks of Ulmus davidiana var. japonica, were evaluated for inhibitory activity against the formation of AGEs. Compounds 1 - 5 exhibited a significant inhibitory activity on the formation of AGEs in an AGEs-BSA assay by specific fluorescence and this was confirmed by an indirect AGEs-ELISA. Moreover, compounds 1 and 5 markedly reduced AGEs-BSA cross-linking to collagen in a dose-dependent manner. AGEs:advanced glycation end products BSA:bovine serum albumin CC:column chromatography CD:circular dichroism.
Lee
Ga Young
GY
Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
Jang
Dae Sik
DS
Kim
Junghyun
J
Kim
Chan-Sik
CS
Kim
Young Sook
YS
Kim
Joo-Hwan
JH
Kim
Jin Sook
JS
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000037:antagonists & inhibitors; D034403:Ulmus / Q000737:chemistry*
D005419:Flavonoids; D017127:Glycation End Products, Advanced; D010936:Plant Extracts; C081628:advanced glycation end products-bovine serum albumin; D012710:Serum Albumin, Bovine; C404987:flavan-3-ol; D003094:Collagen