title: A biofunctional polymeric coating for microcantilever molecular recognition.
abstract: An innovative route to activate silicon microcantilevers (MCs) for label free molecular recognition is presented. The method consists in coating the underivatized MCs with a functional ter-polymer based on N,N-dimethylacrylamide (DMA) bearing N-acryloyloxysuccinimide (NAS) and 3-(trimethoxysilyl)propyl-methacrylate (MAPS), two functional monomers that confer to the polymer the ability to react with nucleophilic species on biomolecules and with glass silanols, respectively. The polymer was deposited onto MCs by dip coating. Polymer coated MCs were tested in both static and dynamic modes of actuation, featuring detection of DNA hybridization as well as protein/protein interaction. In the dynamic experiments, focused on protein detection, the MCs showed an average mass responsivity of 0.4 Hz/pg for the first resonant mode and of 2.5 Hz/pg for the second resonant mode. The results of the static experiments, dedicated to DNA hybridization detection, allowed for direct estimation of the DNA duplex formation energetics, which resulted fully consistent with the nominal expected values. These results, together with easiness and cheapness, high versatility, and excellent stability of the recognition signal, make the presented route a reliable alternative to standard SAM functionalization (for microcantilevers (MCs) and for micro-electro-mechanical systems (MEMS) in general).
authors.affiliation: Chemistry for Technologies Laboratory, University of Brescia, Brescia, Italy. giulio.oliviero@ing.unibs.it
authors.forename: Giulio
authors.initials: G
authors.lastname: Oliviero
authors.forename: Paolo
authors.initials: P
authors.lastname: Bergese
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Canavese
authors.forename: Marcella
authors.initials: M
authors.lastname: Chiari
authors.forename: Paolo
authors.initials: P
authors.lastname: Colombi
authors.forename: Marina
authors.initials: M
authors.lastname: Cretich
authors.forename: Francesco
authors.initials: F
authors.lastname: Damin
authors.forename: Sonia
authors.initials: S
authors.lastname: Fiorilli
authors.forename: Simone L
authors.initials: SL
authors.lastname: Marasso
authors.forename: Carlo
authors.initials: C
authors.lastname: Ricciardi
authors.forename: Paola
authors.initials: P
authors.lastname: Rivolo
authors.forename: Laura E
authors.initials: LE
authors.lastname: Depero
mesh_terms: D000178:Acrylamides / Q000138:chemical synthesis; D000179:Acrylates / Q000138:chemical synthesis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000378:metabolism; D008689:Methacrylates / Q000138:chemical synthesis; D009693:Nucleic Acid Hybridization; D017646:Organosilicon Compounds / Q000138:chemical synthesis; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D013388:Succinimides / Q000138:chemical synthesis
chemical_list: D000178:Acrylamides; D000179:Acrylates; D008689:Methacrylates; D017646:Organosilicon Compounds; D011108:Polymers; D011506:Proteins; D013388:Succinimides; D004247:DNA; C099046:N,N-dimethylacrylamide; C505504:3-(trimethoxysilyl)propyl methacrylate