title: Oxidative stress profile in the post-operative patients with biliary atresia.
abstract: OBJECTIVE
  Many post-operative patients with biliary atresia (BA) suffer from liver dysfunction, such as chronic inflammation even without jaundice after a Kasai's hepatic portoenterostomy.
  
  
  METHODS
  The presence and degree of oxidative stress were evaluated in the post-operative patients with BA. Twelve outpatients who underwent a Kasai's hepatic portoenterostomy were evaluated. The active oxygen products, the rate of bioantioxidant, the markers of oxidative stress, and the degree of hepatic oxidative stress were examined by immunohistochemical staining of biopsied specimens.
  
  
  RESULTS
  All of the oxidative stress markers in the post-operative patients with BA increased in comparison to those in the controls. Moreover, 8-OHdG immunohistochemical staining was positive in 84+/-4.8% in hepatic cells in the portal area in the post-operative patients with BA.
  
  
  CONCLUSIONS
  The post-operative patients with BA were under increased oxidative stress, even if their liver dysfunction was mild without jaundice. Antioxidant therapy might be necessary to decrease of oxidative stress in the post-operative patients with BA.
authors.affiliation: Department of Pediatric Surgery, Kurume University School of Medicine, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan. monmon@ka3.so-net.ne.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Asakawa
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Kimio
authors.initials: K
authors.lastname: Asagiri
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Ken
authors.initials: K
authors.lastname: Tanikawa
authors.forename: Minoru
authors.initials: M
authors.lastname: Yagi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000410:Alanine Transaminase / Q000378:metabolism; D001656:Biliary Atresia / Q000378:metabolism* / Q000601:surgery*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D024141:Collagen Type IV / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D015237:Dinoprost / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D011171:Portoenterostomy, Hepatic; D011184:Postoperative Period; D013482:Superoxide Dismutase / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D024141:Collagen Type IV; C075750:8-epi-prostaglandin F2alpha; D007334:Insulin-Like Growth Factor I; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D013482:Superoxide Dismutase; D005723:gamma-Glutamyltransferase; D000410:Alanine Transaminase; D003849:Deoxyguanosine