title: Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin.
abstract: The ratio of hapten and bovine serum albumin in an antigenconjugate was determined by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Ahybridoma secreting monoclonal antibody against ginsenosideRg(1) was produced by fusing sprenocytes immunized with aginsenoside Rg(1)-bovine serum albumin conjugate withHAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very smallcross-reaction appeared with ginsenoside Re. The full measuringrange of the assay extends from 0.3 mug ml(-1) to 10 mug ml(-1) ofginsenoside Rg(1).
authors.affiliation: Department of Pharmacognosy, Graduate school of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higahsi-ku, Fukuoka, 812,-8582, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Fukuda
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Shoyama