Interaction of anticancer drug methotrexate with nucleic acids analyzed by multi-spectroscopic method.
Methotrexate (MTX) as an antifolate, which is widely used as chemotherapeutic drugs. A high-dose MTX therapy has a direct toxicity influence on the non-germinal cells, especially the liver cells. It is known that the inject dose for adults is 10-30 mg and is half for children for routine use, while our experiments showed that the optimum dosage of MTX which enhanced the RLS intensities to the maximum is 4.54 ng ml(-1). The interaction of methotrexate (MTX) with nucleic acids in aqueous solution in the presence of cetyltrimethylammonium bromide (CTMAB), a kind of cationic surfactant similar to the Human cells, were investigated based on the measurements of resonance light scattering (RLS), UV-vis, fluorescence and NMR spectra, etc. The interaction has been proved to give a ternary complex of MTX-CTMAB-DNA in BR buffer (pH 9.30), which exhibits strong enhanced RLS signals at 339.5 nm.
Cai
Changqun
C
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, PR China. cai_mao3@hotmail.com
Chen
Xiaoming
X
Gong
Hang
H
D000970:Antineoplastic Agents / Q000737:chemistry*; D002021:Buffers; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008727:Methotrexate / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D009994:Osmolar Concentration; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry
D000970:Antineoplastic Agents; D002021:Buffers; D002593:Cetrimonium Compounds; D009696:Nucleic Acids; D013501:Surface-Active Agents; D008727:Methotrexate; D000077286:Cetrimonium