title: [Molecular identification of medicinal plant genus Uncaria in Guizhou].
abstract: OBJECTIVE
  To analyze rDNA ITS regions of the Medicinal Plant Genus Uncaria in Guizhou and construct their phylogenetic tree in order to supply molecular evidence of taxonomy and identification of these Medicinal Plants in genetic level.
  
  
  METHODS
  The ITS gene fragments of the 4 Medicinal Plants were PCR amplified and sequenced. The rDNA ITS regions were analyzed by means of the software of ClustalX, BioEdit and PAUP* 4.0 beta 10.
  
  
  RESULTS
  The entire sequences of rDNA ITS1, ITS2, and 5.8S rDNA were obtained, The Maximum-parsimony tree of four ITS regions together with those of similar sequences from GenBank were found, as Mitrayna rubrostipulata (AJ492621 ) and Mitragyna rubrostipulata (AJ605988) were designated as outgroup.
  
  
  CONCLUSIONS
  The 4 medicinal plants are the 4 species in the genus Uncaria, and are mostly similar to the Uncaria rhynhcophylla.
authors.affiliation: The Key Laboratory for Agri. Biotechnology of Guizhou, Guiyang 550006, China. ttg729@sina.com
authors.forename: Tao
authors.initials: T
authors.lastname: Gang
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Zuo-Yi
authors.initials: ZY
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction / Q000379:methods; D018749:RNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D032070:Uncaria / Q000145:classification / Q000235:genetics* / Q000254:growth & development
chemical_list: D004275:DNA, Ribosomal; D018749:RNA, Plant