On-line flow injection-cloud point preconcentration of polycyclic aromatic hydrocarbons coupled with high-performance liquid chromatography.
Cloud point methodology has been used to develop a novel preconcentration and an analytical method for polycyclic aromatic hydrocarbons (PAHs) in soil sample. The nonionic surfactant Tergitol 15-S-7 was successfully used as the surfactant-mediated extractant in both ultrasonic and microwave-assisted extractions. Over 90% of recoveries for various PAHs in soil were obtained under the optimal experimental conditions. The extracts were then preconcentrated and analyzed by our proposed on-line coupling method "flow injection-cloud point preconcentration-high-performance liquid chromatography" (FI-CPP-HPLC) equipped with a fluorescence detector and an excitation/emission wavelength program. The preconcentration system and optimal working conditions were established. The limit of detection of the FI-CPE-HPLC system ranges from 0.101 to 0.456 microg/L for the selected PAHs, i.e., phenanthrene, pyrene, chrysene, benzo[k]fluoranthene and benzo[a]pyrene. Our proposed technique provides a reliable, simple and automatic analytical method for the determination of PAHs in environmental soil samples.
Li
Cheuk Fai
CF
Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, China.
Wong
Jonathan W C
JW
Huie
Carmen W
CW
Choi
Martin M F
MM
D000645:Ammonium Sulfate / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005233:Fatty Alcohols / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis* / Q000302:isolation & purification
D005233:Fatty Alcohols; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D000645:Ammonium Sulfate