title: The CellScan technology for in vitro studies on novel platinum complexes with organoarsenic ligands.
abstract: The present paper examines one of the multiple uses of the CellScan technique, its utilization in the biological evaluation of novel compounds, in order to improve the methods concerning the prediction of their anti-proliferative effects and application as anti-cancer drugs. The CellScan is a laser scanning static cytometer, enabling repetitive spectroscopic measurements in intact living cells. The detection of cell viability and apoptosis is possible, based on the transformations that occur in the cytoplasm matrix of tumour cells influenced by cytotoxic compounds. The measurement of fluorescence changes, due to this phenomenon, is possible with the CellScan system. The potential of this technology to detect the in vitro effects of the inhibitory molecules on tumour cells was demonstrated, making this method a valuable tool for chemosensitivity tests. We synthesized and fully characterised three novel platinum complexes of tertiary arsine ligands: trans-[PtI2(2-iPrOC6H4AsPh2)2] (), trans-[PtCl2(2-MeOC6H4AsPh2)2] () and cis-[PtCl2(2-HOC6H4AsPh2)2] (). The three compounds are biologically active against tumour cells and their cytotoxicity is comparable with standard drugs. Measurements using the CellScan technology correlate well with the results provided by other bioassay methods.
authors.affiliation: Tumor Biology Laboratory, Research Department, I. Chiricuta Cancer Institute, 34-36 Republici St., 400015, Cluj Napoca, Romania. fischer.eva@iocn.ro
authors.forename: Eva
authors.initials: E
authors.lastname: Fischer-Fodor
authors.forename: Natalia
authors.initials: N
authors.lastname: Moldovan
authors.forename: Piroska
authors.initials: P
authors.lastname: Virag
authors.forename: Olga
authors.initials: O
authors.lastname: Soritau
authors.forename: Ioana
authors.initials: I
authors.lastname: Brie
authors.forename: Peter
authors.initials: P
authors.lastname: Lönnecke
authors.forename: Evamarie
authors.initials: E
authors.lastname: Hey-Hawkins
authors.forename: Luminita
authors.initials: L
authors.lastname: Silaghi-Dumitrescu
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001152:Arsenicals / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D020552:Comet Assay; D018360:Crystallography, X-Ray; D003470:Culture Media; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007834:Lasers; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D009153:Mutagens / Q000633:toxicity; D009944:Organoplatinum Compounds / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D003470:Culture Media; D008024:Ligands; D009153:Mutagens; D009944:Organoplatinum Compounds; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue