title: Rapid detection of anti-chromatin autoantibodies in human serum using a portable electrochemical biosensor.
abstract: The current system employed electrochemical sensor technology for the measurement of autoantibodies in human sera. Anti-chromatin antibodies are an early and sensitive indicator of systemic lupus erythematosus (SLE) and are typically quantified using enzyme-linked immunosorbent assays (ELISA). Electrochemical detection compared favorably with ELISA using a sample of 30 SLE sera (r=0.9), and non-specific binding by normal serum immunoglobulin was undetectable. The electrochemical sensor assay required <20min processing time and utilized a hand-held apparatus with a disposable electrode. These results demonstrate the applicability of this technology to the rapid measurement of a clinically relevant analyte with an apparatus of potential simplicity and low cost.
authors.affiliation: Dept. of Internal Medicine, University of New Mexico Health Sciences Center, USA.
authors.forename: Konstantin N
authors.initials: KN
authors.lastname: Konstantinov
authors.forename: Ravil A
authors.initials: RA
authors.lastname: Sitdikov
authors.forename: Gabriel P
authors.initials: GP
authors.lastname: Lopez
authors.forename: Plamen
authors.initials: P
authors.lastname: Atanassov
authors.forename: Robert L
authors.initials: RL
authors.lastname: Rubin
mesh_terms: D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D002843:Chromatin / Q000276:immunology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D002843:Chromatin