title: Entamoeba histolytica: effect on virulence, growth and gene expression in response to monoxenic culture with Escherichia coli 055.
abstract: Monoxenic cultivation of pathogenic Entamoeba histolytica trophozoites with Escherichia coli serotype 055 which binds strongly to the Gal/GalNAc amoebic lectin, markedly improved the growth of E. histolytica and produced a significant decrease in cysteine proteinase activity and a lower cytopathic activity on monolayer cells after 3 months of monoxenic culture. However, after long term monoxenic culture (12 months) the proteolytic and cytopathic activities were recovered and the amoebic growth reached the maximum yield. Employing the GeneFishing(R) technology and DNA macroarrays we detected differentially gene expression related to the amoebic interaction with bacteria. A number of differentially expressed genes encoding metabolic enzymes, ribosomal proteins, virulence factors and proteins related with cytoskeletal and vesicle trafficking were found. These results suggest that E. coli 055 has a nutritional role that strongly supports the amoebic growth, and is also able to modulate some biological activities related with amoebic virulence.
authors.affiliation: Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, Gto, Mexico.
authors.forename: Claudia Leticia
authors.initials: CL
authors.lastname: Mendoza-Macías
authors.forename: Minerva Paola
authors.initials: MP
authors.lastname: Barrios-Ceballos
authors.forename: Lydia Patricia Cárdenas
authors.initials: LP
authors.lastname: de la Peña
authors.forename: Angeles
authors.initials: A
authors.lastname: Rangel-Serrano
authors.forename: Fernando
authors.initials: F
authors.lastname: Anaya-Velázquez
authors.forename: David
authors.initials: D
authors.lastname: Mirelman
authors.forename: Felipe
authors.initials: F
authors.lastname: Padilla-Vaca
mesh_terms: D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000096:biosynthesis; D016054:DNA, Protozoan / Q000737:chemistry; D004748:Entamoeba histolytica / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D004926:Escherichia coli / Q000254:growth & development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014774:Virulence
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D016054:DNA, Protozoan; D016053:RNA, Protozoan; D003546:Cysteine Endopeptidases