title: Grape seed extract Vitis vinifera protects against radiation-induced oxidative damage and metabolic disorders in rats.
abstract: Whole body exposure to ionizing radiation induces the formation of reactive oxygen species (ROS) in different tissues provoking oxidative damage, organ dysfunction and metabolic disturbances. The present study was designed to determine the possible protective effect of grape seed extract (GSE), rich in proanthocyanidins against gamma-radiation-induced oxidative stress in heart and pancreas tissues associated with serum metabolic disturbances. Irradiated rats were whole body exposed to 5 Gy gamma-radiation. GSE-treated irradiated rats received 100 mg GSE/kg/day, by gavage, for 14 days before irradiation. The animals were killed on days 1, 14 and 28 after irradiation. Significant decreases of SOD, CAT and GSH-Px activities associated with significant increases of TBARS levels were recorded in both tissues after irradiation. GSE administration pre-irradiation significantly attenuated the radiation-induced oxidative stress in heart tissues which was substantiated by a significant amelioration of serum LDH, CPK and AST activities. GSE treatment also attenuated the oxidative stress in pancreas tissues which was associated with a significant improvement in radiation-induced hyperglycemia and hyperinsulinemia. In conclusion, the present data demonstrate that GSE would protect the heart and pancreas tissues from oxidative damage induced by ionizing irradiation.
authors.affiliation: Department of Radiation Biology, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt. helensaada@hotmail.com
authors.forename: Helen N
authors.initials: HN
authors.lastname: Saada
authors.forename: Ussama Z
authors.initials: UZ
authors.lastname: Said
authors.forename: Nefissa H
authors.initials: NH
authors.lastname: Meky
authors.forename: Afrag S
authors.initials: AS
authors.lastname: Abd El Azime
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001774:Blood Chemical Analysis; D005720:Gamma Rays / Q000009:adverse effects*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects; D010179:Pancreas / Q000378:metabolism / Q000528:radiation effects; D010936:Plant Extracts / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000517:prevention & control; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D027843:Vitis
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D044945:Proanthocyanidins; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances