title: The effect of carbenoxolone on hippocampal formation theta rhythm in rats: in vitro and in vivo approaches.
abstract: The role of gap junction (GJ) coupling in the generation of hippocampal formation theta rhythm was investigated in vitro, with use of brain slices, and in vivo, with use of urethane anesthetized rats. Carbenoxolone, the succinyl ester of glycyrrhetinic acid, and GJ blocker reversibly abolished hippocampal formation theta rhythm recorded in slice preparations and urethane anesthetized rats. The present study yielded novel data which demonstrated that the pattern of delay in blockage of theta rhythm after carbenoxolone treatment, and the pattern of theta recovery after administration of this agent, require a specific time period (2-3h for delay and 8-12h for recovery), one that can be demonstrated using different experimental protocols.
authors.affiliation: Department of Neurobiology, The University of Łódź, Łódź 90-222, Rewolucji 1905 st. No. 66, Poland. renab@biol.uni.lodz.pl
authors.forename: Renata
authors.initials: R
authors.lastname: Bocian
authors.forename: Anna
authors.initials: A
authors.lastname: Posłuszny
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kowalczyk
authors.forename: Henryk
authors.initials: H
authors.lastname: Gołebiewski
authors.forename: Jan
authors.initials: J
authors.lastname: Konopacki
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002217:Carbachol / Q000494:pharmacology; D002229:Carbenoxolone / Q000494:pharmacology*; D017629:Gap Junctions / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D013826:Theta Rhythm / Q000187:drug effects*
chemical_list: D002217:Carbachol; D002229:Carbenoxolone