Fatty acids, unusual glycophospholipids and DNA analyses of thermophilic bacteria isolated from hot springs.
The composition of fatty acids in 12 strains of the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus was analyzed by gas chromatography-mass spectrometry. Major FAs found in the profiles included i-15:0, i-17:0, ai-15:0, i-16:0, 16:0, ai-17:0, together with some minor components. Branched FAs were predominant, forming more than 80% of all FAs measured. Fast atom bombardment-mass spectrometry was used for analysis of unusual glycophospholipids, i.e., acylglycosylcardiolipins from genera Geobacillus and Alicyclobacillus and 1-(hydroxy(2-(O-acylglycosyl-oxy)hexadecyloxy)phosphoryloxy) hexadecan-2-yl esters of C15-C17 acids from genera Thermus and Meiothermus. Cloning and preliminary sequence analysis of 16S rDNA showed that these isolates belong to the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus.
Siristova
Lucie
L
Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology Prague, Technicka 3, 166 28 Prague, Czech Republic. lucie.siristova@vscht.cz
Melzoch
Karel
K
Rezanka
Tomas
T
D001419:Bacteria / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005227:Fatty Acids / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000302:isolation & purification*; D006358:Hot Temperature; D010743:Phospholipids / Q000302:isolation & purification*; D013045:Species Specificity; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids