Microsomal prostaglandin E synthase-2 is not essential for in vivo prostaglandin E2 biosynthesis.
Prostaglandin E(2) (PGE(2)) plays an important role in the normal physiology of many organ systems. Increased levels of this lipid mediator are associated with many disease states, and it potently regulates inflammatory responses. Three enzymes capable of in vitro synthesis of PGE(2) from the cyclooxygenase metabolite PGH(2) have been described. Here, we examine the contribution of one of these enzymes to PGE(2) production, mPges-2, which encodes microsomal prostaglandin synthase-2 (mPGES-2), by generating mice homozygous for the null allele of this gene. Loss of mPges-2 expression did not result in a measurable decrease in PGE(2) levels in any tissue or cell type examined from healthy mice. Taken together, analysis of the mPGES-2 deficient mouse lines does not substantiate the contention that mPGES-2 is a PGE(2) synthase.
Jania
Leigh A
LA
Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7264, USA.
Chandrasekharan
Subhashini
S
Backlund
Michael G
MG
Foley
Nicholas A
NA
Snouwaert
John
J
Wang
I-Ming
IM
Clark
Patsy
P
Audoly
Laurent P
LP
Koller
Beverly H
BH
D000818:Animals; D015152:Blotting, Northern; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000502:physiology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D000071876:Prostaglandin-E Synthases
D008565:Membrane Proteins; D007371:Interferon-gamma; C077623:Ptgs2 protein, mouse; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496512:Ptgs1 protein, mouse; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; C000605866:Ptges2 protein, mouse; D015232:Dinoprostone