"HOOF-Print" genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale.
We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus population genetic data to help discriminate among microbial isolates such as Brucella.
Almendra
Cláudia
C
CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal. claudia_almendra@mail.icav.up.pt
Silva
Teresa Luísa
TL
Beja-Pereira
Albano
A
Ferreira
Ana Cristina
AC
Ferrão-Beck
Lénia
L
de Sá
Maria Inácia Corrêa
MI
Bricker
Betsy J
BJ
Luikart
Gordon
G
D015373:Bacterial Typing Techniques / Q000379:methods*; D002002:Brucella / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017347:Brucella melitensis / Q000235:genetics*; D002006:Brucellosis / Q000382:microbiology; D005798:Genes, Bacterial; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D018598:Minisatellite Repeats; D008957:Models, Genetic; D010802:Phylogeny; D011174:Portugal