title: Dendritic cell-associated immune inflammation of cardiac mucosa: a possible factor in the formation of Barrett's esophagus.
abstract: BACKGROUND
  The development of Barrett's esophagus is poorly understood, but it has been suggested that cardiac mucosa is a precursor of intestinal type metaplasia and that inflammation of cardiac mucosa may play a role in the formation of Barrett's esophagus. The present study was undertaken to examine the presence and distribution of immune-inflammatory cells in cardiac mucosa, specifically focusing on dendritic cells because of their importance as regulators of immune reactions.
  
  
  METHODS
  Endoscopic biopsy specimens were obtained from 12 patients with cardiac mucosa without Barrett's esophagus or adenocarcinoma and from 21 patients with Barrett's esophagus without dysplasia (intestinal metaplasia). According to histology, in nine of the 21 specimens with Barrett's esophagus, areas of mucosa composed of cardiac type epithelium-lined glands were present as well. Immunohistochemical staining and electron microscopy were used to examine immune-inflammatory cells in paraffin-embedded sections.
  
  
  RESULTS
  Immune-inflammatory cells, including T cells, B cells, dendritic cells, macrophages, and mast cells, were present in the connective tissue matrix that surrounded cardiac type epithelium-lined glands in all patients with cardiac mucosa. Clustering of dendritic cells with each other and with lymphocytes and the intrusion of dendritic cells between glandular mucus cells were observed. In the Barrett's esophagus specimens that contained cardiac type glands, computerized CD83 expression quantitation revealed that there were more dendritic cells in cardiac mucosa than in intestinal metaplasia.
  
  
  CONCLUSIONS
  Immune-inflammatory infiltrates containing dendritic cells are consistently present in cardiac mucosa. The finding of a larger number of dendritic cells in areas of cardiac mucosa in Barrett's esophagus biopsies suggests that the immune inflammation of cardiac mucosa might play a role in modifying the local tissue environment to promote the development of specialized intestinal type metaplasia.
authors.affiliation: Department of Surgery and Centre for Immunology, St. Vincent's Hospital, University of New South Wales, Sydney, Australia. y.bobryshev@unsw.edu.au
authors.forename: Yuri V
authors.initials: YV
authors.lastname: Bobryshev
authors.forename: Dinh
authors.initials: D
authors.lastname: Tran
authors.forename: Murray C
authors.initials: MC
authors.lastname: Killingsworth
authors.forename: Michael
authors.initials: M
authors.lastname: Buckland
authors.forename: Reginald V N
authors.initials: RV
authors.lastname: Lord
mesh_terms: D001471:Barrett Esophagus / Q000276:immunology* / Q000473:pathology*; D016022:Case-Control Studies; D003713:Dendritic Cells / Q000502:physiology*; D006801:Humans; D007111:Immunity, Cellular / Q000502:physiology; D018928:Immunity, Mucosal / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology; D008264:Macrophages / Q000502:physiology; D008407:Mast Cells / Q000502:physiology; D009092:Mucous Membrane / Q000473:pathology; D009206:Myocardium / Q000473:pathology*