A novel method and simple apparatus for the detection of thermophilic Campylobacter spp. in chicken meat products.
Conventional procedures for isolation of thermophilic Campylobacter spp. from chicken are complex, labor intensive, and time-consuming. The objective of this study was to create a novel Campylobacter culturing apparatus. A main concept of the device was based on the ability of Campylobacter to pass through a 0.45 microm pore size filter in viscous media. Preliminary study demonstrated that only viable Campylobacter moved through the membrane filter and could multiply in the enrichment culture. C. jejuni, C. coli, C. lari, and C. upsaliensis in the chicken samples were detected at cell concentrations as low as 10 cfu/g, after 24 h incubation at 42 degrees C. In total, 84 retail chicken samples were comparatively studied using both conventional method and apparatus. Sixteen samples (19.05%) were positive by the apparatus method; 14 (16.66%) of these positive samples contained C. coli and 2 (2.38%) contained C. jejuni. With the conventional method, 7 (8.33%) samples were positive 7 (8.33%) with C. coli. In conclusion, the apparatus detected more positive samples than did the conventional culture method.
Wisessombat
Sueptrakool
S
Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Kittiniyom
Kanokwan
K
Srimanote
Potjanee
P
Wonglumsom
Wijit
W
Voravuthikunchai
Supayang P
SP
D000818:Animals; D002169:Campylobacter Infections / Q000635:transmission*; D017000:Campylobacter coli / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification; D044863:Campylobacter lari / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification; D044885:Campylobacter upsaliensis / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification; D002645:Chickens; D003257:Consumer Product Safety; D003470:Culture Media; D005374:Filtration / Q000379:methods; D005506:Food Contamination; D005516:Food Microbiology; D050296:Microbial Viability; D008851:Micropore Filters / Q000382:microbiology; D011201:Poultry Diseases / Q000382:microbiology*; D011202:Poultry Products / Q000382:microbiology*; D012680:Sensitivity and Specificity
D003470:Culture Media