title: Determination of a hydrophilic paclitaxel derivative, 7-xylosyl-10-deacetylpaclitaxel in rat plasma by LC-MS/MS.
abstract: A LC-MS/MS method for the determination of a hydrophilic paclitaxel derivative 7-xylosyl-10-deacetylpaclitaxel in rat plasma was developed to evaluate the pharmacokinetics of 7-xylosyl-10-deacetylpaclitaxel in the rats. 7-Xylosyl-10-deacetylpaclitaxel and docetaxel (IS for 7-xylosyl-10-deacetylpaclitaxel) were extracted from rat plasma with acetic ether and analyzed on a Hypersil C(18 )column (4.6 x 150 mm i.d., particle size 5 microm) with the mobile phase of ACN/0.05% formic acid (50:50, v/v). The analytes were detected using an ESI MS/MS in the multiple reaction monitoring mode. The standard curves for 7-xylosyl-10-deacetylpaclitaxel in plasma were linear (r > 0.999) over the concentration range of 2.0-1000 ng/mL with a weighting of 1/concentration(2). The method showed a satisfactory sensitivity (2.0 ng/mL using 50 microL plasma), precision (CV <or= 10.1%), accuracy (relative error -12.4 to 12.0%), and selectivity. This method was successfully applied to the pharmacokinetic study of 7-xylosyl-10-deacetylpaclitaxel in rat plasma after intravenous administration of 7-xylosyl-10-deacetylpaclitaxel to female Wistar rats.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, People's Republic of China.
authors.forename: Shou Gang
authors.initials: SG
authors.lastname: Jiang
authors.forename: Yuan Gang
authors.initials: YG
authors.lastname: Zu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Yu Jie
authors.initials: YJ
authors.lastname: Fu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Hua
authors.forename: Jing Tao
authors.initials: JT
authors.lastname: Wang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077143:Docetaxel; D005260:Female; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D043823:Taxoids / Q000097:blood; D013997:Time Factors
chemical_list: C531111:7-xylosyl-10-deacetylpaclitaxel; D043823:Taxoids; D000077143:Docetaxel; D017239:Paclitaxel