Analysis of HCV-specific T cells by flow cytometry.
Flow cytometry has become an essential research tool because of the increase in the number of its {applications.} The development of an increasing number of monoclonal antibodies (mAbs) and fluorochromes, and of instruments capable of multicolor detection, allows the acquisition of a large amount of phenotypic and functional information in a single assay. In addition, flow-cytometry techniques have overcome critical problems of conventional assays, such as the use of radioactive reagents to assess proliferation and cytotoxicity of virus-specific T cells. Here, we provide both an overview of available techniques as well as standard protocols that have proven valuable in the assessment of HCV-specific T-cell responses.
Shiina
Masaaki
M
Immunology Section, Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney diseases, National Institutes of Health, Bethesda, MD, USA.
Rehermann
Barbara
B
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D006680:HLA Antigens / Q000378:metabolism; D016174:Hepacivirus / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism; D013045:Species Specificity; D013194:Staining and Labeling; D013388:Succinimides / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D006680:HLA Antigens; D013388:Succinimides; D007371:Interferon-gamma; D001973:Bromodeoxyuridine