title: DNA repair after DNA fragmentation in mouse small intestinal epithelial cells.
abstract: In our earlier work, we found that, in mice, i.p. injection of anti-CD3 monoclonal antibody activated intraepithelial lymphocytes (iIEL), leading to DNA fragmentation in villous epithelial cells of the duodenum and jejunum within 30 min. By 2 h after injection, nearly half of the enterocytes had detached from the villi, and DNA fragmentation could barely be detected in the remaining villous epithelium. We hypothesized that DNA had been repaired in enterocytes in which DNA fragmentation had previously been induced. In this study, enterocytes became negative for TUNEL staining at 60 min after anti-CD3 treatment, prior to detachment. The remaining villous epithelial cells, after DNA fragmentation and detachment, were found to be positive for 5-bromo-2-deoxyuridine labeling. To confirm whether fragmented DNA had been repaired in situ, we investigated the appearance and/or mobilization of DNA-repair-related proteins. Focus formation, a typical staining pattern of repair-related proteins including phosphorylated H2AX, phospo-ATM substrate, and Nbs1, was observed 30 min after anti-CD3 injection, with the kinetics virtually identical to that of DNA fragmentation. The co-localization of gamma-H2AX and phospo-ATM substrate was also confirmed. The disappearance of a positive reaction for TUNEL staining in previously fragmented DNA, the appearance of representative DNA-repair-related proteins, the coincidence of the kinetics of DNA fragmentation and this appearance of DNA-repair-related proteins, and the co-localization of two of the repair-related proteins strongly indicated that enterocyte DNA could be repaired after it had been fragmented in vivo. Thus, DNA fragmentation per se may not necessarily be an immediate sign of cell death.
authors.affiliation: Division of Immunology and Embryology, Department of Cell Biology, Tohoku University School of Medicine, 980-8575 Sendai, Japan. ogata@immem.med.tohoku.ac.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Ogata
authors.forename: Takehiko
authors.initials: T
authors.lastname: Oomori
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Soga
authors.forename: Yuta
authors.initials: Y
authors.lastname: Ota
authors.forename: Ari
authors.initials: A
authors.lastname: Itoh
authors.forename: Takaji
authors.initials: T
authors.lastname: Matsutani
authors.forename: Masanobu
authors.initials: M
authors.lastname: Nanno
authors.forename: Ryuji
authors.initials: R
authors.lastname: Suzuki
authors.forename: Tsunetoshi
authors.initials: T
authors.lastname: Itoh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D061067:Antibodies, Monoclonal, Humanized; D018797:Cell Cycle Proteins / Q000378:metabolism*; D053938:DNA Fragmentation; D004260:DNA Repair; D004268:DNA-Binding Proteins; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006657:Histones / Q000378:metabolism*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D009687:Nuclear Proteins / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D006657:Histones; D009687:Nuclear Proteins; C466108:Nijmegen breakage syndrome 1 protein, mouse; C456519:visilizumab; C475961:gamma-H2AX protein, mouse