Comparative transcriptome analysis of arsenate and arsenite stresses in rice seedlings.
The effect of arsenic (As) exposure on genome-wide expression was examined in rice (Oryza sativa L., ssp. Indica). A group of defense and stress-responsive genes, transporters, heat-shock proteins, metallothioneins, sulfate-metabolizing proteins, and regulatory genes showed differential expression in rice seedlings challenged with arsenate (AsV) and arsenite (AsIII). AsV stress led to upregulation or downregulation of an additional set of genes in comparison to AsIII. Differential expression of several genes that showed the highest contrast in a microarray analysis was validated by following the quantitative changes in the levels of individual transcripts following challenge with AsV, AsIII, Cd, Cr, and Pb. Most of the selected genes responded to challenge by heavy metals such as arsenic. However, expression of one of the cytochrome P450 genes (Os01g43740) in rice root was induced by AsV but not by other heavy metals. Similarly, one glutaredoxin (Os01g26912) is expressed specifically in the AsIII-treated shoot.
Chakrabarty
Debasis
D
National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, UP, India.
Trivedi
Prabodh Kumar
PK
Misra
Prashant
P
Tiwari
Manish
M
Shri
Manju
M
Shukla
Devesh
D
Kumar
Smita
S
Rai
Arti
A
Pandey
Ashutosh
A
Nigam
Deepti
D
Tripathi
Rudra Dev
RD
Tuli
Rakesh
R
D001149:Arsenates / Q000494:pharmacology*; D018053:Arsenites / Q000494:pharmacology*; D020869:Gene Expression Profiling; D017343:Genes, Plant; D018745:Genome, Plant; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological
D001149:Arsenates; D018053:Arsenites; D006360:Heat-Shock Proteins; D006540:Herbicides; D010940:Plant Proteins; C015001:arsenite; C025657:arsenic acid