[Isolation of Id1 interaction protein in human prostate cancer].
OBJECTIVE
To isolate the inhibitor of DNA binding 1 (Id1) interaction protein and to determine the role and action mechanism of Id1 in human prostate cancer.


METHODS
The expression vector pET-28a/Id1 was established and used as a bait to prey the interaction protein by pull-down assay.


RESULTS
A clear interaction protein band was observed by SDS-PAGE, which was found to be activating transcription factor 3 (ATF3) by Western blotting.


CONCLUSIONS
Id1 may play a role in human prostate cancer by interacting with ATF3.
Yu
Xiao-Ling
XL
Department of Pathophysiology, Medical College of Qingdao University, Qingdao, Shandong 266021, China. xiaoling_yu2004@163.com
Xie
Jun-Xia
JX
D051700:Activating Transcription Factor 3 / Q000032:analysis / Q000378:metabolism; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D012313:RNA; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051700:Activating Transcription Factor 3; D051798:Inhibitor of Differentiation Protein 1; D012313:RNA