[A comparison study of post-cerebral hemorrhage stress ulcer with two interventional treatments aimed at endogenous endothelin in rat].
OBJECTIVE
To observe the respective effects of intervention either with endothelin (ET) antibody or with ET receptor antagonist on acute stress ulcer (ASU) subsequent to cerebral hemorrhage in rats.


METHODS
Forty Sprague-Dawley (SD) rats were randomly divided into control group (group A, n=10), model group (group B, n=10), ET antibody (group C, n=10), and ET receptor antagonist group (group D, n=10). Right intracerebral hemorrhage was reproduced by injection of 200 microl autologous venous blood. Normal saline, ET antibody, or ET receptor antagonist was respectively administered intravenously per day for designated group. The rats were sacrificed at 3 days of the experiment. The incidence of ASU and ulcer index were assessed, serum ET-1 level, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in serum, Rsvmit (Rsv) and Vvmit (Vv) of cerebral and gastric mucosa were measured, and pathological examination of the cerebral tissue and gastric mucosa was performed with light microscopy and electron microscopy.


RESULTS
In group B, serum ET-1 level did not changed. MDA content were markedly increased in serum, cerebral and gastric mucosa, SOD activity were markedly decreased, cerebral water content were markedly increased; Rsv in neuron and gastric parietal cell, Vv in gastric parietal cell both were markedly decreased (P<0.05 or P<0.01). ASU was only observed in group B (the incidence was 30%, ulcer index was 15). It was not observed in other groups. Compared with group B, MDA content were significantly decreased, and SOD activity were significantly increased in serum, cerebral and gastric mucosa in groups C and D, cerebral water content in group C were dramatically decreased (all P<0.01), but these were not statistically different between groups C and D. Rsv and Vv in neuron and gastric parietal cell in groups B, C and D were not statistically different, and serum ET-1 level were not statistically different among the groups (all P>0.05).


CONCLUSIONS
Intervention of ET antibody and ET receptor antagonist can both reduce occurrence and development of ASU subsequent to cerebral hemorrhage in rats.
Huang
Xing-qing
XQ
Department of Emergency, The Affiliated Yuebei People's Hospital of Shantou University Medical College, Shaoguan 512026, Guangdong, China. david-huangxq@163.com
Rao
Wen-lin
WL
Liu
Xiao-yan
XY
Zhong
Yan-dong
YD
Lin
Tuo
T
Zhang
Chang-rui
CR
Zhu
Biao
B
D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use; D000906:Antibodies / Q000627:therapeutic use; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002543:Cerebral Hemorrhage / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1 / Q000097:blood; D016232:Endothelins / Q000276:immunology*; D005753:Gastric Mucosa / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010437:Peptic Ulcer / Q000209:etiology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism
D000897:Anti-Ulcer Agents; D000906:Antibodies; D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1; D016232:Endothelins; D008315:Malondialdehyde; D013482:Superoxide Dismutase