title: Quantitative analysis of the responses of murine bone marrow mesenchymal stem cells to EGF, PDGF-BB and fibronectin by factorial design methodology.
abstract: A 2-level full factorial design was firstly employed to explore the responses of murine bone marrow mesenchymal stem cells stimulated by various combinations of EGF, PDGF-BB, and fibronectin. EGF and PDGF-BB individually, rather than fibronectin, had significant effects on cell proliferation. The synergism between PDGF-BB and fibronectin, as well as the antagonism between EGF and PDGF-BB were also detected. Moreover, cells changed from a flattened and spread to a smaller and elongated shape with addition of factors. The factors also moved cells out of G0/G1 phase, increasing the fractions of cells in S and G2/M phases.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, Peoples Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Geng
authors.forename: Haiying
authors.initials: H
authors.lastname: Sun
authors.forename: Fengshan
authors.initials: F
authors.lastname: Luo
authors.forename: Nianmin
authors.initials: N
authors.lastname: Qi