The application and use of chemical space mapping to interpret crystallization screening results.
Macromolecular crystallization screening is an empirical process. It often begins by setting up experiments with a number of chemically diverse cocktails designed to sample chemical space known to promote crystallization. Where a potential crystal is seen a refined screen is set up, optimizing around that condition. By using an incomplete factorial sampling of chemical space to formulate the cocktails and presenting the results graphically, it is possible to readily identify trends relevant to crystallization, coarsely sample the phase diagram and help guide the optimization process. In this paper, chemical space mapping is applied to both single macromolecules and to a diverse set of macromolecules in order to illustrate how visual information is more readily understood and assimilated than the same information presented textually.
Snell
Edward H
EH
Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, NY 14203, USA. esnell@hwi.buffalo.edu
Nagel
Ray M
RM
Wojtaszcyk
Ann
A
O'Neill
Hugh
H
Wolfley
Jennifer L
JL
Luft
Joseph R
JR
D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D046911:Macromolecular Substances / Q000737:chemistry*; D008956:Models, Chemical; D012984:Software
D046911:Macromolecular Substances