title: Presence of ten isoforms of monocarboxylate transporter (MCT) family in the bovine adrenal gland.
abstract: This study provides novel information regarding the existence and precise cellular localization of various monocarboxylate transporters (MCTs) in the mammalian adrenal gland. RT-PCR results revealed that 10 MCT isoforms, namely MCT1, MCT2, MCT3, MCT4, MCT5, MCT8, MCT9, MCT10, MCT13, and MCT14 are expressed in the bovine adrenal gland. MCTs (MCT1-MCT8) proteins were examined by Western blot analysis in the bovine adrenal gland. The precise cellular localization of six MCT isoforms (MCT1-MCT5 and MCT8) within the different zones of the adrenal gland has been determined by immunohistochemical and immunofluorescence confocal laser microscopy analyses. To gain insight on the species differences for the expression profiles of MCT isoforms in this vital organ, we also examined the expression and cellular localization of MCT1-MCT8 in the rat adrenal gland. Some discrepancies in MCTs profiles between cattle and rat have been observed in the different zones of the adrenal gland. The tissue distribution pattern of MCT isoforms in the steroid-secreting adrenal cortex and catecholamine-secreting adrenal medulla suggests that they may play distinct roles in the regulation of the different hormone biosynthesis in the adrenal gland. Also, it is possible that different MCT isoforms in adrenal gland can be differentially regulated under acute or chronic conditions. This report can form the basis for future research on the regulation of these transporters in the adrenal gland.
authors.affiliation: Department of Veterinary Physiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. doaakirat@hotmail.com
authors.forename: Doaa
authors.initials: D
authors.lastname: Kirat
authors.forename: Khalid
authors.initials: K
authors.lastname: Sallam
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hayashi
authors.forename: Taku
authors.initials: T
authors.lastname: Miyasho
authors.forename: Seiyu
authors.initials: S
authors.lastname: Kato
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D027501:Monocarboxylic Acid Transporters; D020033:Protein Isoforms