title: The Mdm2 ubiquitin ligase enhances transcriptional activity of human papillomavirus E2.
abstract: The regulation of human papillomavirus (HPV) gene expression by the E2 protein is a critical feature of the viral life cycle. Previous studies have shown an important role in transcription for the ubiquitin-proteasome pathway, but its role in HPV gene expression has not been addressed. We now show that HPV E2 requires an active proteasome for its optimal transcriptional activator function. This involves an interaction with the Mdm2 ubiquitin ligase, which together with E2 acts synergistically to activate the HPV type 16 promoter. We also show that HPV E2 recruits Mdm2 onto HPV promoter sequences, providing an explanation for this cooperative activity.
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34012 Trieste, Italy.
authors.forename: Noor
authors.initials: N
authors.lastname: Gammoh
authors.forename: Daniela
authors.initials: D
authors.lastname: Gardiol
authors.forename: Paola
authors.initials: P
authors.lastname: Massimi
authors.forename: Lawrence
authors.initials: L
authors.lastname: Banks
mesh_terms: D006801:Humans; D027383:Papillomaviridae / Q000502:physiology*; D051736:Proto-Oncogene Proteins c-mdm2 / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2