title: Determination of epoxyeicosatrienoic acids in human red blood cells and plasma by GC/MS in the NICI mode.
abstract: Epoxyeicosatrienoic acids (EETs) are cytochrome P450 metabolites of arachidonic acid involved in the regulation of vascular tone. Despite the importance of EETs in a variety of physiological effects, few methods have been developed to quantify them in human blood. This led us to develop a method by GC/MS with negative ion chemical ionization. As EETs are primarily located in phospholipids, red blood cells (RBCs) and plasma phospholipids were hydrolyzed with phospholipase A(2) after a solid phase extraction. Then, EETs were derivatized as pentafluorobenzyl esters, and [(2)H(8)]-arachidonic acid was used as internal standard for quantification. EETs were found to be at concentrations of 106+/-37ng mL(-1) in plasma and 33.4+/-8.5 ng/10(9) RBCs (mean+/-S.D.) in 10 healthy volunteers. Their amount in RBCs was 3-fold that in plasma; both parameters proved to be well correlated.
authors.affiliation: Université de Brest, INSERM U613, ECLA, Brest, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Goulitquer
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Dréano
authors.forename: François
authors.initials: F
authors.lastname: Berthou
authors.forename: Laurent
authors.initials: L
authors.lastname: Corcos
authors.forename: Danièle
authors.initials: D
authors.lastname: Lucas
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives / Q000097:blood; D015777:Eicosanoids / Q000097:blood*; D004912:Erythrocytes / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D010949:Plasma / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D035501:Uncertainty
chemical_list: D015777:Eicosanoids; D015126:8,11,14-Eicosatrienoic Acid