title: Androgen receptor is expressed in murine choroid plexus and downregulated by 5alpha-dihydrotestosterone in male and female mice.
abstract: The choroid plexuses (CPs) of the brain form a unique interface between the peripheral blood and the cerebrospinal fluid (CSF). CPs produce several neuroprotective peptides, which are secreted into the CSF. Despite their importance in neuroprotection, the mechanisms underlying the regulation of most of these peptides in CPs remain unknown. Androgens regulate the expression of neuroprotective peptides in several tissues where the androgen receptor (AR) is coexpressed, including the brain. The presence of AR in CPs has never been investigated, but recent studies in our laboratory show that the CP is an androgen-responsive tissue. In order to fulfill this gap, we investigated and characterized AR distribution and expression in male and female rat CPs and in primary cultures from rat CP epithelial cells. In addition, the response of AR to 5alpha-dihydrotestosterone (DHT) in castrated male and female mice subjected to DHT replacement was analyzed. We show that rat CP epithelial cells contain AR mRNA and protein. Moreover, we demonstrate that AR is downregulated by DHT in mice CPs.
authors.affiliation: Research Center in Health Sciences-CICS, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.
authors.forename: C Henrique
authors.initials: CH
authors.lastname: Alves
authors.forename: Isabel
authors.initials: I
authors.lastname: Gonçalves
authors.forename: Sílvia
authors.initials: S
authors.lastname: Socorro
authors.forename: Graça
authors.initials: G
authors.lastname: Baltazar
authors.forename: Telma
authors.initials: T
authors.lastname: Quintela
authors.forename: Cecília R A
authors.initials: CR
authors.lastname: Santos
mesh_terms: D000728:Androgens / Q000494:pharmacology; D000818:Animals; D002831:Choroid Plexus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000494:pharmacology*; D015536:Down-Regulation; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000728:Androgens; D012333:RNA, Messenger; D011944:Receptors, Androgen; D013196:Dihydrotestosterone