title: S-nitrosylation of AtSABP3 antagonizes the expression of plant immunity.
abstract: Changes in cellular redox status are a well established response across phyla following pathogen challenge. In this context, the synthesis of nitric oxide (NO) is a conspicuous feature of plants responding to attempted microbial infection and this redox-based regulator underpins the development of plant immunity. However, the associated molecular mechanism(s) have not been defined. Here we show that NO accretion during the nitrosative burst promotes increasing S-nitrosylation of the Arabidopsis thaliana salicylic acid-binding protein 3 (AtSABP3) at cysteine (Cys) 280, suppressing both binding of the immune activator, salicylic acid (SA), and the carbonic anhydrase (CA) activity of this protein. The CA function of AtSABP3 is required for the expression of resistance in the host against attempted pathogen infection. Therefore, inhibition of AtSBAP3 CA function by S-nitrosylation could contribute to a negative feedback loop that modulates the plant defense response. Thus, AtSABP3 is one of the first targets for S-nitrosylation in plants for which the biological function of this redox-based post-translational modification has been uncovered. These data provide a molecular connection between the changes in NO levels triggered by attempted pathogen infection and the expression of disease resistance.
authors.affiliation: Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
authors.forename: Yi-Qin
authors.initials: YQ
authors.lastname: Wang
authors.forename: Angela
authors.initials: A
authors.lastname: Feechan
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Yun
authors.forename: Reza
authors.initials: R
authors.lastname: Shafiei
authors.forename: Andreas
authors.initials: A
authors.lastname: Hofmann
authors.forename: Paul
authors.initials: P
authors.lastname: Taylor
authors.forename: Peng
authors.initials: P
authors.lastname: Xue
authors.forename: Fu-Quan
authors.initials: FQ
authors.lastname: Yang
authors.forename: Zhen-Sheng
authors.initials: ZS
authors.lastname: Xie
authors.forename: Jacqueline A
authors.initials: JA
authors.lastname: Pallas
authors.forename: Cheng-Cai
authors.initials: CC
authors.lastname: Chu
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Loake
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry; D008958:Models, Molecular; D009589:Nitrogen Oxides / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology; D011485:Protein Binding; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary
chemical_list: D029681:Arabidopsis Proteins; D009589:Nitrogen Oxides; D002256:Carbonic Anhydrases; C539168:SABP3 protein, Arabidopsis