title: Status of platelet-lymphocyte aggregation in circulating blood of patients with type 1 diabetes with and without diabetic nephropathy.
abstract: BACKGROUND
  Platelets can modulate the role of lymphocytes in the development of micro- and macrovascular complications in type 1 diabetes mellitus.
  
  
  OBJECTIVE
  To clarify the status of platelet-lymphocyte aggregation in circulating blood in patients with T1DM, as well as the differences in the platelet-lymphocyte aggregation in T1DM patients with and without diabetic nephropathy.
  
  
  METHODS
  We recruited 115 T1DM patients (47 men and 68 women) aged 15-52 years. The subjects with mean albumin excretion > or = 5 microg/mg creatinine comprised group 1, and those with < 5 microg/mg creatinine comprised group 2. The matched healthy participants (n=50) served as the control group. Detection of LPA was achieved using a light microscope after Ficoll-gradient centrifugation. Immunophenotyping of lymphocytes was performed by flow cytometry.
  
  
  RESULTS
  Significantly more LPA (430.4 +/- 20.6/microl) were observed in group 2 compared with group 1 (223.9 +/- 12.8/microl, P< 0.001) and the control group (296.1 +/- 22.6/microl, P=0.027). In group 1 significantly more LPA/CD4 (21.1 +/- 1.6%) and LPA/(CD4 + NK) (17.8 +/- 1.7%) were found than in group 2 and the control group.
  
  
  CONCLUSIONS
  T1DM with diabetic nephropathy is associated with higher levels of LPA than T1MD without diabetic nephropathy. The role of LPA in microvascular complications in diabetes should be elucidated in further studies.
authors.affiliation: Department of Physiology, Chita Medical Academy, Chita, Russia.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Vitkovsky
authors.forename: Boris
authors.initials: B
authors.lastname: Kuznik
authors.forename: Alexey
authors.initials: A
authors.lastname: Solpov
authors.forename: Eli
authors.initials: E
authors.lastname: Magen
mesh_terms: D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000502:physiology*; D002449:Cell Aggregation / Q000502:physiology; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000150:complications; D003928:Diabetic Nephropathies / Q000097:blood* / Q000209:etiology; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000502:physiology*; D055815:Young Adult