Use of a surrogate chimeric virus to detect West Nile virus-neutralizing antibodies in avian and equine sera.
A chimeric yellow fever virus/West Nile virus (WNV) was compared to WNV alone as a biosafety level 2 reagent in the plaque reduction neutralization test for determining WNV infection histories. Concordance was 96.3% among 188 avian and equine serum samples. Neutralizing antibody titers were frequently more than twofold lower with the chimera.
Komar
Nicholas
N
CDC-NCZVED-DVBID-ADB, 3150 Rampart Rd., Fort Collins, CO 80521, USA. nkomar@cdc.gov
Langevin
Stanley
S
Monath
Thomas P
TP
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001715:Bird Diseases / Q000175:diagnosis*; D001717:Birds; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D009500:Neutralization Tests; D011995:Recombination, Genetic; D010948:Viral Plaque Assay; D014901:West Nile Fever / Q000662:veterinary*; D014902:West Nile virus / Q000276:immunology; D015005:Yellow fever virus / Q000235:genetics
D000914:Antibodies, Viral