Comparative hydrolysis and fermentation of sugarcane and agave bagasse.
Sugarcane and agave bagasse samples were hydrolyzed with either mineral acids (HCl), commercial glucanases or a combined treatment consisting of alkaline delignification followed by enzymatic hydrolysis. Acid hydrolysis of sugar cane bagasse yielded a higher level of reducing sugars (37.21% for depithed bagasse and 35.37% for pith bagasse), when compared to metzal or metzontete (agave pinecone and leaves, 5.02% and 9.91%, respectively). An optimized enzyme formulation was used to process sugar cane bagasse, which contained Celluclast, Novozyme and Viscozyme L. From alkaline-enzymatic hydrolysis of sugarcane bagasse samples, a reduced level of reducing sugar yield was obtained (11-20%) compared to agave bagasse (12-58%). Selected hydrolyzates were fermented with a non-recombinant strain of Saccharomyces cerevisiae. Maximum alcohol yield by fermentation (32.6%) was obtained from the hydrolyzate of sugarcane depithed bagasse. Hydrolyzed agave waste residues provide an increased glucose decreased xylose product useful for biotechnological conversion.
Hernández-Salas
J M
JM
Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, Exhacienda San Juan Molino, Tepetitla de Lardizábal, Tlaxcala, Mexico.
Villa-Ramírez
M S
MS
Veloz-Rendón
J S
JS
Rivera-Hernández
K N
KN
González-César
R A
RA
Plascencia-Espinosa
M A
MA
Trejo-Estrada
S R
SR
D027882:Agave / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D031786:Saccharum / Q000737:chemistry / Q000378:metabolism*
D000431:Ethanol; D002482:Cellulose; C027433:bagasse