[Studies on chemical constituents and antibacterial activity from n-butanol extract of Sarcandra glabra].
OBJECTIVE
To study the chemical constituents and the antibacterial activity from n-butanol extract of Sarcandra glabra.


METHODS
The compounds were isolated by Diaion HP-20, Sephadex LH-20, MCI CHP-20 and silica gel column chromatographic methods. Their structures were identified on the basis of physicochemical and spectroscopic analysis. The antibacterial effect of the compounds were measured against Staphylococcus aureus by filterpaper slice method, finally the antibacterial ring in each group was recorded after 24 hours.


RESULTS
Seven constituents were isolated and elucidated as 5, 7, 3', 4'-tetrahydroxy-6-C-beta-D-glucopyranosylflavanone (1), kaempferol-3-O-beta-D-glucuronide (2), fraxidin (3), isofraxidin (4), isofraxidin-7-O-beta-D-glucopyranoside (5), kaempferol (6), pinostrobin (7). Diameters (in mm) of antibacterial ring in the compounds 2, 5, 6 were orderly recorded as follows: 14.67 +/- 0.08, 11.14 +/- 1.06, 8.26 +/- 1.26 and the compound 4 is not effective.


CONCLUSIONS
Compounds 1-3 and 5 were isolated from S. glabra for the first time.
Yuan
Ke
K
Research and Development Center of Natural Medicine, Zhejiang Forestry University, Linan 311300, China. yuan_ke001@163.com
Zhu
Jian-xin
JX
Si
Jin-ping
JP
Cai
Hai-ke
HK
Ding
Xiao-dan
XD
Pan
Yuan-jiang
YJ
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000440:Butanols / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000440:Butanols; D004365:Drugs, Chinese Herbal