[Comparative analysis of components absorbed into blood after oral administration of Liuwei Dihuang Wan and its related prescriptions].
OBJECTIVE
To compare the compoents absorbed into blood after oral administration of Liuwei Dihuang Wan, Zhibai Dihuang Wan and Guifu Dihuang Wan, and to elucidate the composing principle of the prescriptions related to Liuwei Dihuang Wan.


METHODS
Using the method of RP-HPLC, the serum chromatograms of rats after oral administration of the three prescriptions were established. By comparing the chromatograms with that of the prescriptions and composing drugs, the origin and the difference of compounds absorbed into blood of Liuwei Dihuang Wan and the other two prescriptions were identified.


RESULTS
Fourteen compounds were detected in all of the serum after administration of three prescriptions reapectly. Additionally, five special compounds were detected in the serum of Zhibai Dihuang Wan, and two special compounds were detected in the serum of Guifu Dihuang Wan.


CONCLUSIONS
The number of compounds absorbed into blood reflects the different effects of the three prescriptions, and it should be the prospective access to elucidate the composing principle of formula.
Wang
Xi-jun
XJ
School of Pharmacy, Heilongliang University of Chinese Medicine, Harbin 150040, China. wxj@hljucm.net
Zhang
Ning
N
Sun
Hui
H
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D005260:Female; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; C476907:liu-wei-di-huang wan