title: Improvement of L-lactate production by CYB2 gene disruption in a recombinant Saccharomyces cerevisiae strain under low pH condition.
abstract: Using a DNA microarray, we found that expression of the genes related to lactate metabolism was upregulated in a lactate-producing recombinant Saccharomyces cerevisiae strain. Disruption of the CYB2 gene encoding L-lactate dehydrogenase improved the L-lactate production by S. cerevisiae under low pH condition.
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Ookubo
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirasawa
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nagahisa
authors.forename: Chikara
authors.initials: C
authors.lastname: Furusawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D005818:Genetic Engineering; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
chemical_list: D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase