title: [Screening on alkaloid of Sophora alopecuraides against adenocarcinoma of colon cell line SW620 in vitro].
abstract: OBJECTIVE
  To test 5 kinds of alkaloid of Sophora alopecuraides L., the effects of TBSA, MT, OMT, SC, SRI against adenocarcinoma of colon cell line SW620.
  
  
  METHODS
  SW620 cell line was treated with alkaloid at various concentrations. The effect on cell proliferative activity was measured by MTT assay and the effects of alkaloids against cell growth were compared. The changes of cell morphology were observed through optical microscope and fluorescence microscope and the cell apoptosis was detected by flow cytometry and Annexin V-FITC apoptosis assay after the treatments.
  
  
  RESULTS
  According to the results of MTT assay, all of the 5 kinds of alkaloid especially SRI and MT were identified to produce growth inhibition against SW620 cells. The sequence of the intensity of effect was TBSA < OMT < SC < MT < SRI. Further study of SRI and MT demonstrated their effects on inducing SW620 cell apoptosis, and SRI showed the strongest effect. Both SRI and MT induced SW620 cells to apoptosis in a dose-and-time-dependent manner.
  
  
  CONCLUSIONS
  The 5 kinds of alkaloid especially SRI and MT may inhibit the growth of SW620 cells,and induce SW620 cell apoptosis. The results of this study pave the way for further identification of the effective components in Sophora alopecuraides L. that inhibit colorectal carcinoma both in vivo and in vitro.
authors.affiliation: School of TCM, Southern Medical University, Guangzhou 510515, China. libraliang78@126.com
authors.forename: Lei
authors.initials: L
authors.lastname: Liang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Wang
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Zhang
authors.forename: Wen-Chang
authors.initials: WC
authors.lastname: Zhao
authors.forename: Hong-Zhu
authors.initials: HZ
authors.lastname: Deng
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000648:ultrastructure; D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D015179:Colorectal Neoplasms / Q000473:pathology / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006801:Humans; D011807:Quinolizines / Q000494:pharmacology; D029909:Sophora / Q000737:chemistry; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D011807:Quinolizines; D000093842:Matrines