title: Stable expression of recombinant human coagulation factor XIII in protein-free suspension culture of Chinese hamster ovary cells.
abstract: The recombinant a and bsubunits for human coagulation factor XIII were transfected into Chinese hamster ovary (CHO) cells. CHO cells were amplified and selected with methotrexate in adherent cultures containing serum, and CHO 1-62 cells were later selected in protein-free medium. To develop a recombinant factor XIII production process in a suspension culture, we have investigated the growth characteristics of CHO cells and the maintenance of factor XIII expression in the culture medium. Suspension adaptation of CHO cells was performed in protein-free medium, GC-CHO-PI, by two methods, such as serum weaning and direct switching from serum containing media to protein-free media. Although the growth of CHO cells in suspension culture was affected initially by serum depletion, cell specific productivity of factor XIII showed only minor changes by the direct switching to protein-free medium during a suspension culture. As for the long-term stability of factor XIII, CHO 1-62 cells showed a stable expression of factor XIII in protein-free condition for 1000 h. These results indicate that the CHO 1-62cells can be adapted to express recombinant human factor XIII in a stable maimer in suspension culture using a protein-free medium. Our results demonstrate that enhanced cell growth in a continuous manner is achievable for factor XIII production in a protein-free medium when a perfusion bioreactor culture system with a spin filter is employed.
authors.affiliation: Korea Green Cross Corp, 227 Kugal-Ri, Keeheung-Eup, 449-900, Yongin, Korea.
authors.forename: B H
authors.initials: BH
authors.lastname: Chun
authors.forename: W G
authors.initials: WG
authors.lastname: Bang
authors.forename: Y K
authors.initials: YK
authors.lastname: Park
authors.forename: S K
authors.initials: SK
authors.lastname: Woo