title: Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai.
abstract: The marine mollusk Aplysia is a useful model organism for studying the cellular bases of behavior and plasticity. However, molecular studies of Aplysia have been limited by the lack of genomic information. Recently, a large scale characterization of neuronal transcripts was performed in A. californica. Here, we report the analysis of a parallel set of neuronal transcripts from a closely related species A. kurodai found in the northwestern Pacific. We collected 4,859 nonredundant sequences from the nervous system tissue of A. kurodai. By performing microarray and real-time PCR analyses, we found that ApC/EBP, matrilin, antistasin, and eIF3e clones were significantly up-regulated and a BAT1 homologous clone was significantly down-regulated by 5-HT treatment. Among these, we further demonstrated that the Ap-eIF3e plays a key role in 5-HT-induced long-term facilitation (LTF) as a positive regulator.
authors.affiliation: National Creative Research Initiative, Center for Memory and Institute of Molecular Biology and Genetics, Department of Biological Sciences, College of Natural Sciences, Seoul National University, San 56-1 Silim-dong, Gwanak-gu, Seoul 151-747, Korea.
authors.forename: Yong-Seok
authors.initials: YS
authors.lastname: Lee
authors.forename: Sun-Lim
authors.initials: SL
authors.lastname: Choi
authors.forename: Tae-Hyung
authors.initials: TH
authors.lastname: Kim
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Lee
authors.forename: Hyong Kyu
authors.initials: HK
authors.lastname: Kim
authors.forename: Hyoung
authors.initials: H
authors.lastname: Kim
authors.forename: Deok-Jin
authors.initials: DJ
authors.lastname: Jang
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Lee
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Lee
authors.forename: Gwang Sik
authors.initials: GS
authors.lastname: Sin
authors.forename: Chang-Bae
authors.initials: CB
authors.lastname: Kim
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Tai
authors.initials: T
authors.lastname: Kubo
authors.forename: Leonid L
authors.initials: LL
authors.lastname: Moroz
authors.forename: Eric R
authors.initials: ER
authors.lastname: Kandel
authors.forename: Jong
authors.initials: J
authors.lastname: Bhak
authors.forename: Bong-Kiun
authors.initials: BK
authors.lastname: Kaang
mesh_terms: D000818:Animals; D001048:Aplysia / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D017931:DNA Primers; D020224:Expressed Sequence Tags; D017774:Long-Term Potentiation / Q000187:drug effects; D009473:Neuronal Plasticity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D012701:Serotonin / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D012701:Serotonin