Improved catalytic activity of a purified multienzyme from a modular polyketide synthase after coexpression with Streptomyces chaperonins in Escherichia coli.
Betancor
Lorena
L
Department of Biochemistry, University of Cambridge, Cambridge, UK.
Fernández
María-José
MJ
Weissman
Kira J
KJ
Leadlay
Peter F
PF
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018835:Chaperonin 10 / Q000378:metabolism; D018834:Chaperonin 60 / Q000378:metabolism; D018833:Chaperonins / Q000235:genetics / Q000378:metabolism*; D002942:Circular Dichroism; D004917:Erythromycin / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics*; D048630:Polyketide Synthases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000235:genetics; D053719:Tandem Mass Spectrometry
D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D011994:Recombinant Proteins; D004917:Erythromycin; D048630:Polyketide Synthases; D018833:Chaperonins