title: Two cases of recovery of dimorphic pathogenic fungi via conventional BacT/ALERT microbial detection system media.
abstract: We hereby report two instances of dimorphic fungus cultivation in BacT/ALERT-based bacteriologic media, with the first such characterization of Blastomyces dermatitidis. From a patient with disseminated coccidioidomycosis, routine blood cultures incubated on the MB/BacT 3D Microbial Detection System generated a positive signal following 75 h of incubation. B. dermatitidis was isolated from a patient hospitalized with a four-week course of respiratory illness. Organism detection from respiratory specimens via the MB/BacT 3D Mycobacteria Detection System occurred 5 days sooner than the routine fungus culture. Etiologic agents of endemic mycoses may be isolated in bacteriologic media employed by continuous monitoring instrumentation.
authors.affiliation: Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, WI 53226, USA. Erik.Munson@wfhc.org
authors.forename: Erik
authors.initials: E
authors.lastname: Munson
authors.forename: Tina
authors.initials: T
authors.lastname: Endes
authors.forename: Katharine
authors.initials: K
authors.lastname: Vaughan
authors.forename: Timothy
authors.initials: T
authors.lastname: Block
authors.forename: Jeanne E
authors.initials: JE
authors.lastname: Hryciuk
mesh_terms: D000328:Adult; D001431:Bacteriological Techniques / Q000295:instrumentation*; D001758:Blastomyces / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D001759:Blastomycosis / Q000175:diagnosis / Q000382:microbiology; D001769:Blood / Q000382:microbiology*; D003045:Coccidioides / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D003047:Coccidioidomycosis / Q000175:diagnosis / Q000382:microbiology; D003470:Culture Media; D016469:Fungemia / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D008172:Lung Diseases, Fungal / Q000175:diagnosis / Q000382:microbiology; D008297:Male; D012137:Respiratory System / Q000382:microbiology*; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods; D013997:Time Factors
chemical_list: D003470:Culture Media