title: Overexpression of insulin-like growth factor binding protein 3 in oral squamous cell carcinoma.
abstract: Previously, we established an in vitro cellular carcinogenesis model of oral squamous cell carcinoma (OSCC), including a human immortalized oral epithelial cell (HIOEC) line and its derived cancerous HB96 cell line. Further cDNA microarray analysis showed a significant up-regulated gene, insulin-like growth factor binding protein 3 (IGFBP3), accompanying with in vitro cancerization from HIOEC to HB96. In order to investigate IGFBP3 up-regulation and its potential usefulness as a molecular marker in OSCC, we detected the IGFBP3 expression with a panel of OSCC lines, and clinical samples of cancerous tissues and paired adjacent non-malignant epithelia from primary OSCC patients. Western blotting and real-time PCR showed increased IGFBP3 mRNA level and protein expression in OSCC cell lines compared with HIOEC in vitro; immunohistochemistry and real-time PCR also showed increased IGFBP3 mRNA level and protein expression in cancerous tissues compared with adjacent non-malignant epithelia from OSCC patients. Positive correlations were found between the IGFBP3 protein-positive grade in cancerous tissue and the tumor size as well as lymph node metastasis, a larger tumor size and positive lymph node metastasis indicating a higher level of IGFBP3 protein-positive grade. Based on these results, IGFBP3 may be used as a positive biomarker for OSCC development and progression.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, P.R. China.
authors.forename: Lai-Ping
authors.initials: LP
authors.lastname: Zhong
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Kui-Jie
authors.initials: KJ
authors.lastname: Wei
authors.forename: Hong-Ya
authors.initials: HY
authors.lastname: Pan
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Zhou
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.forename: Wan-Tao
authors.initials: WT
authors.lastname: Chen
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Zhang
mesh_terms: D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D045744:Cell Line, Tumor; D004848:Epithelium / Q000196:embryology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008954:Models, Biological; D009062:Mouth Neoplasms / Q000378:metabolism*; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D012333:RNA, Messenger