Effect of ThinPrep preparation on human papillomavirus detection and genotyping in rectal samples by PCR.
Specimen-to-specimen carryover during ThinPrep slide preparation was evaluated by comparing human papillomavirus genotypes detected prior and subsequent to the ThinPrep processing of 121 PreservCyt samples. Overall, 52 samples generated concordant genotypes and 38 had additional and 21 had fewer genotypes postprocessing. PreservCyt samples should be aliquoted for PCR testing prior to ThinPrep processing.
Tabrizi
Sepehr N
SN
Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia. sepehr.tabrizi@rch.org.au
Stevens
Matthew P
MP
van Leeuwen
Marina T
MT
Vajdic
Claire M
CM
Botes
Leon P
LP
Medley
Gabriele
G
Hillman
Richard
R
Grulich
Andrew
A
Garland
Suzanne M
SM
D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D008297:Male; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*
D004279:DNA, Viral