Identity tests: determination of cell line cross-contamination.
Cell line cross-contamination is a phenomenon that arises as a result of the continuous cell line culture. It has been estimated that around 20% of the cell lines are misidentified, therefore it is necessary to carry out quality control tests for the detection of this issue. Since cell line cross-contamination discovery, different methods have been applied, such as isoenzyme analysis for inter-species cross-contamination; HLA typing, and DNA fingerprinting using short tandem repeat and a variable number of tandem repeat for intra-species cross-contamination. The cell banks in this sense represent the organizations responsible for guaranteeing the authenticity of cell lines for future research and clinical uses.
Cabrera
C M
CM
Stem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves, Avenida de las Fuerzas Armadas No. 2, 18014, Granada, Spain, mcabrm@fundacionhvn.org.
Cobo
F
F
Nieto
A
A
Cortés
J L
JL
Montes
R M
RM
Catalina
P
P
Concha
A
A