title: Gene delivery in vitro and in vivo from bioreducible multilayered polyelectrolyte films of plasmid DNA.
abstract: Layer-by-layer (LbL) films were assembled on flexible stainless steel substrate using plasmid DNA and reducible hyperbranched poly(amido amine) (RHB) polycation. The films were characterized by XPS and their disassembly in reducing conditions confirmed by ellipsometry. Fibroblast and smooth muscle cell attachment and proliferation on DNA/RHB films were indistinguishable from those on control DNA/poly(ethylenimine) (PEI) films. In vitro transfection activity was evaluated using reporter plasmids encoding for secreted alkaline phosphatase (SEAP) and green fluorescent protein (GFP). DNA/RHB films showed higher and longer lasting transfection activity than control DNA/PEI films using SEAP plasmid. It was revealed through the use of GFP plasmid that DNA/RHB films transfected almost the entire cell population growing on the films. In vivo transfection activity was evaluated by subcutaneously implanting a stainless steel substrate coated with the DNA/RHB films containing SEAP plasmid DNA and measuring the levels of SEAP secreted into the blood circulation of rats. It was found that the plasma levels of SEAP peaked at approximately 160 ng SEAP/mL five days post-implantation.
authors.affiliation: Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Jenifer
authors.initials: J
authors.lastname: Blacklock
authors.forename: Ye-Zi
authors.initials: YZ
authors.lastname: You
authors.forename: Qing-Hui
authors.initials: QH
authors.lastname: Zhou
authors.forename: Guangzhao
authors.initials: G
authors.lastname: Mao
authors.forename: David
authors.initials: D
authors.lastname: Oupický
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004573:Electrolytes / Q000737:chemistry*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D004573:Electrolytes; D011073:Polyamines; D011108:Polymers; D049452:Green Fluorescent Proteins; D004247:DNA; D000469:Alkaline Phosphatase