title: Does comparative genomic hybridization reveal distinct differences in DNA copy number sequence patterns between leiomyosarcoma and malignant fibrous histiocytoma?
abstract: Leiomyosarcoma (LMS) is the third most common type of soft tissue sarcoma after malignant fibrous histiocytoma (MFH) and liposarcoma. Comparative genomic hybridization (CGH) has shown similar DNA copy number imbalances in LMS and MFH. It has been suggested that both tumors may correspond to different differentiation states of a single tumor entity and that a large proportion of MFHs could correspond to undifferentiated LMS. We report CGH results from 102 MFH and 82 LMS cases, as well as a subsequent clustering analysis. The distribution pattern of DNA copy number changes could not differentiate LMS from MFH, suggesting that most MFHs could represent an ultimate state of tumor progression of LMS. Even if an oncogenic pattern common to LMS and MFH is valid, the genes relevant to smooth muscle cell differentiation may reside in one or more chromosomal imbalances that are not shared by both tumor types. Further explorative analysis identified a small cluster of tumors (9% of the samples: 2 LMS and 10 MFH) characterized by the presence of high-level amplifications at 1p33 approximately p34.3, 17q22 approximately q23, 17q25 approximately qter, 19p, 22p, and 22q, and associated with a higher proportion of tumors located in the thigh (P=0.003) and with male sex (P=0.079).
authors.affiliation: Department of Pathology, Haartman Institute and HUSLAB, University of Helsinki and Helsinki University Central Hospital, P.O. Box 21 (Haartmaninkatu 3), FI-00014 University of Helsinki, Helsinki, Finland
authors.forename: Marcelo L
authors.initials: ML
authors.lastname: Larramendy
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Gentile
authors.forename: Sonia
authors.initials: S
authors.lastname: Soloneski
authors.forename: Sakari
authors.initials: S
authors.lastname: Knuutila
authors.forename: Tom
authors.initials: T
authors.lastname: Böhling
mesh_terms: D000199:Actins / Q000235:genetics; D000465:Algorithms; D001483:Base Sequence; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000235:genetics; D016000:Cluster Analysis; D004273:DNA, Neoplasm / Q000235:genetics*; D005784:Gene Amplification; D051677:Histiocytoma, Malignant Fibrous / Q000235:genetics*; D006801:Humans; D007890:Leiomyosarcoma / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000235:genetics*
chemical_list: D000199:Actins; D004273:DNA, Neoplasm