[Study on the extraction process of total flavonoids from Hypericum perforatum].
OBJECTIVE
To investigate the factors affecting the extracting total flavonoids from Hypericum perforatum L. systematically.


METHODS
Taken total flavonoids yield as index, the effects of ethanol concentration, extraction temperature, extraction time, extraction times and solvent consumption on total flavonoids yield were investigated separately by single factor test. Orthogonal test was designed by extraction temperature, solvent consumption, ethanol concentration and extraction time.


RESULTS
The factors affecting total flavonoid yield was in the order of extraction temperature, ethanol concentration, extraction times and ethanol consumption. The total flavonoid was the highest extracted in ten times of 60% ethanol for 3 times.


CONCLUSIONS
Process condition is optimized, which forms the scientific and reasonable bases for developing the anti-impressive active parts from Hypericum perforatum L.
Cheng
Li-Hui
LH
Guangdong Pharmaceutical University, Guangzhou 510224, China. lhc822@163.com
Li
Xiao-Meng
XM
D000928:Antidepressive Agents / Q000302:isolation & purification*; D000431:Ethanol / Q000008:administration & dosage / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D020902:Hypericum / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012431:Rutin / Q000032:analysis / Q000737:chemistry; D012997:Solvents / Q000008:administration & dosage; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D000928:Antidepressive Agents; D005419:Flavonoids; D012997:Solvents; D000431:Ethanol; D012431:Rutin