Atlas of gene expression in the developing kidney at microanatomic resolution.
Kidney development is based on differential cell-type-specific expression of a vast number of genes. While multiple critical genes and pathways have been elucidated, a genome-wide analysis of gene expression within individual cellular and anatomic structures is lacking. Accomplishing this could provide significant new insights into fundamental developmental mechanisms such as mesenchymal-epithelial transition, inductive signaling, branching morphogenesis, and segmentation. We describe here a comprehensive gene expression atlas of the developing mouse kidney based on the isolation of each major compartment by either laser capture microdissection or fluorescence-activated cell sorting, followed by microarray profiling. The resulting data agree with known expression patterns and additional in situ hybridizations. This kidney atlas allows a comprehensive analysis of the progression of gene expression states during nephrogenesis, as well as discovery of potential growth factor-receptor interactions. In addition, the results provide deeper insight into the genetic regulatory mechanisms of kidney development.
Brunskill
Eric W
EW
Division of Developmental Biology, Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
Aronow
Bruce J
BJ
Georgas
Kylie
K
Rumballe
Bree
B
Valerius
M Todd
MT
Aronow
Jeremy
J
Kaimal
Vivek
V
Jegga
Anil G
AG
Yu
Jing
J
Grimmond
Sean
S
McMahon
Andrew P
AP
Patterson
Larry T
LT
Little
Melissa H
MH
Potter
S Steven
SS
D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007668:Kidney / Q000196:embryology* / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009399:Nephrons / Q000196:embryology / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis