title: Efficacy of bp26 and bp26/omp31 B. melitensis Rev.1 deletion mutants against Brucella ovis in rams.
abstract: Brucella melitensis Rev.1 is the most effective vaccine against B. ovis infection in sheep but induces antibodies interfering with B. melitensis diagnosis. Brucella BP26 and Omp31 proteins are differential diagnostic antigens. Single or double bp26 and omp31 Rev.1 deletion mutants have been proven effective against B. melitensis in sheep. Here, the CGV26 (deleted in bp26 gene) and CGV2631 (deleted in both bp26 and omp31 genes) mutants have been tested for efficacy against B. ovis in rams. Either inoculated subcutaneously or conjunctivally, both mutants conferred significant protection against B. ovis. The protection induced by CGV26 was similar to that of Rev.1 but significantly higher than that conferred by CGV2631. In conclusion, the CGV26 mutant, in association with the adequate diagnostic strategy, could be a useful alternative to Rev.1 for sheep vaccination against B. ovis infections in those countries performing simultaneously B. melitensis and B. ovis eradication campaigns.
authors.affiliation: Instituto de Agrobiotecnología, CSIC-UPNA-Gobierno de Navarra, Carretera de Mutilva, s/n. 31192, Mutilva Baja, Navarra, Spain. mariajesus.grillo@unavarra.es
authors.forename: M J
authors.initials: MJ
authors.lastname: Grilló
authors.forename: C M
authors.initials: CM
authors.lastname: Marín
authors.forename: M
authors.initials: M
authors.lastname: Barberán
authors.forename: M J
authors.initials: MJ
authors.lastname: de Miguel
authors.forename: K
authors.initials: K
authors.lastname: Laroucau
authors.forename: I
authors.initials: I
authors.lastname: Jacques
authors.forename: J M
authors.initials: JM
authors.lastname: Blasco
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D017347:Brucella melitensis / Q000235:genetics / Q000276:immunology; D042061:Brucella ovis / Q000276:immunology*; D002006:Brucellosis / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D017353:Gene Deletion; D007114:Immunization / Q000662:veterinary; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D009154:Mutation; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D016896:Treatment Outcome
chemical_list: C106040:BP26 protein, Brucella; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008565:Membrane Proteins; C101108:Omp31 protein, Brucella melitensis; C471349:Rev.1 vaccine, Brucella melitensis