Serine protease activity of Cur l 1 from Curvularia lunata augments Th2 response in mice.
BACKGROUND
Studies with mite allergens demonstrated that proteolytic activity augments allergic airway inflammation. This knowledge is limited to few enzyme allergens.


OBJECTIVE
The objective of this study is to investigate the effect of serine protease Cur l 1 from Curvularia lunata in airway inflammation/hyper-responsiveness.


METHODS
Cur l 1 was purified and inactivated using a serine protease inhibitor. Balb/c mice were sensitized with enzymatically active Cur l 1 or C. lunata extract. Sensitized mice were given booster dose on day 14 with active or inactivated Cur l 1. Intranasal challenge was given on day 28, 29, and 30. Airway hyper-responsiveness was measured by plethysmography. Blood, bronchoalveolar lavage fluid (BALF), spleen, and lungs from mice were analyzed for cellular infiltration, immunoglobulins, and cytokine levels.


RESULTS
Mice challenged with enzymatically active Cur l 1 demonstrated significantly higher airway inflammation than inactive Cur l 1 group mice (p < 0.01). There was a significant difference in serum IgE and IgG1 levels among mice immunized with active Cur l 1 and inactive Cur l 1 (p < 0.01). IL-4 and IL-5 were higher in BALF and splenocyte culture supernatant of active Cur l 1 than inactive Cur l 1 mice. Lung histology revealed increased eosinophil infiltration, goblet cell hyperplasia and mucus secretion in active group.


CONCLUSIONS
Proteolytic activity of Cur l 1 plays an important role in airway inflammation and the inactivated Cur l 1 has potential to be explored for immunotherapy.
Tripathi
Prabhanshu
P
Allergy and Immunology Section, Institute of Genomics and Integrative Biology, D.U. North Campus, Mall Road, Delhi 110 007, India.
Kukreja
Neetu
N
Singh
B P
BP
Arora
Naveen
N
D000485:Allergens / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D052179:Antigens, Plant; D001249:Asthma / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D016207:Cytokines / Q000378:metabolism; D004804:Eosinophils / Q000473:pathology; D005260:Female; D005656:Fungal Proteins / Q000276:immunology* / Q000378:metabolism; D007114:Immunization; D007136:Immunoglobulins / Q000097:blood; D008168:Lung / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000276:immunology; D010047:Ovalbumin / Q000378:metabolism; D012697:Serine Endopeptidases / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
D000485:Allergens; D052179:Antigens, Plant; D016207:Cytokines; D005656:Fungal Proteins; D007136:Immunoglobulins; D010047:Ovalbumin; C542813:Cur I 1 allergen, Curvularia lunata; D012697:Serine Endopeptidases