Improvement of L-lactic acid production from Jerusalem artichoke tubers by mixed culture of Aspergillus niger and Lactobacillus sp.
Aspergillus niger SL-09 and Lactobacillus sp. G-02 were used as a mixed culture in a 7-l fermentor to directly form L-lactic acid from Jerusalem artichoke tubers. The synthesis of inulinase and invertase from A. niger SL-09 was enhanced significantly by the inoculation of Lactobacillus sp. G-02 at 12h of culture, which reached 275.6 and 571.8 U/ml in 60 h, over 5-folds higher than that of the culture using single strain. In the following simultaneous saccharification and fermentation procedure, the highest L-lactic acid concentration of 120.5 g/l was obtained in 36 h of the fed-batch fermentation with high conversion efficiency of 94.5%.
Ge
Xiang-Yang
XY
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, Wuxi, JiangSu Province, PR China.
Qian
He
H
Zhang
Wei-Guo
WG
D001234:Aspergillus niger / Q000378:metabolism*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006368:Helianthus / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000378:metabolism*
D003470:Culture Media; D019344:Lactic Acid