Glutamine deamidation of a recombinant monoclonal antibody.
Deamidation of glutamine (Gln) proceeds at a much slower rate than deamidation of asparagine (Asn) residues at peptide level. However, deamidation of Gln residues in native proteins may occur faster because of the impact of protein structure and thus plays a significant role in affecting protein stability. Gln deamidation of a recombinant monoclonal IgG1 antibody was investigated in the current study. Deamidation was determined by a molecular weight increase of 1 Da, a retention time shift on reversed-phase chromatography and tandem mass spectrometric (MS/MS) analysis of the peptides. As expected, Gln residues at different locations in the three-dimensional structure had different susceptibilities to deamidation. Gln deamidation was highly pH dependent with the highest level detected in the sample incubated at pH 9, and lowest level at pH 6 in the pH range from 5 to 9. The detection of significant levels of Gln deamidation suggested that it may play an important role in affecting heterogeneity and stability of recombinant monoclonal antibodies.
Liu
Hongcheng
H
Process Sciences Department, Abbott Bioresearch Center, Worcester, MA 01605, USA. Hongcheng.liu@abbott.com
Gaza-Bulseco
Georgeen
G
Chumsae
Chris
C
D000577:Amides / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D016466:CHO Cells; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D005973:Glutamine / Q000737:chemistry*; D006801:Humans; D009842:Oligopeptides / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D053719:Tandem Mass Spectrometry
D000577:Amides; D000911:Antibodies, Monoclonal; D009842:Oligopeptides; D011994:Recombinant Proteins; D005973:Glutamine