title: Impact of inactivated extracellular proteases on the modified flagellin type III secretion pathway of Bacillus halodurans.
abstract: The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (Deltahag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases.
authors.affiliation: CSIR Biosciences, Box 395, Pretoria 0001, South Africa.
authors.forename: Eldie
authors.initials: E
authors.lastname: Berger
authors.forename: Erika
authors.initials: E
authors.lastname: du Plessis
authors.forename: Isak
authors.initials: I
authors.lastname: Gerber
authors.forename: Michael
authors.initials: M
authors.lastname: Crampton
authors.forename: Nolwandle
authors.initials: N
authors.lastname: Nxumalo
authors.forename: Maureen
authors.initials: M
authors.lastname: Louw
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D005408:Flagellin / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D026901:Membrane Transport Proteins / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D011993:Recombinant Fusion Proteins; D005408:Flagellin; C076733:FlaD protein, Bacteria; D010447:Peptide Hydrolases