title: [Studies on HPLC fingerprint of co-hirudo injection].
abstract: OBJECTIVE
  Using HPLC To determine hypoxanthine in co-hirudo injection for establishing its HPLC fingerprint, and evaluating its internal quality.
  
  
  METHODS
  The chromatographic separation was performed on a Kromasil C18 column (4.6 mm x 250 mm,5 microm). A linear gradient elution with A (0.01 mol x L(-1) x KH2PO4) and B (50% methanol) was used, the flow rate was 0.8 mL x min(-1), the detection wavelength was set at 254 nm, and the column temperature was at normal.
  
  
  RESULTS
  Hypoxanthine was used as the reference substance in the fingerprint of co-hirudo injection, it showed 15 common peaks and theirs similarity threshod was 0.97.
  
  
  CONCLUSIONS
  This method was accurate, repeatable and useful for the quality control of co-hirudo injection.
authors.affiliation: HeiLong iang Key Laboratory of Anti-Fibrosis Biotherapy, Mudanjiang Medical College, Mudanjiang 157011, China. yuanxiao-huan@yahoo.cn
authors.forename: Xiao-huan
authors.initials: XH
authors.lastname: Yuan
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Yang
authors.forename: Chun-tao
authors.initials: CT
authors.lastname: Wang
authors.forename: Guo-mei
authors.initials: GM
authors.lastname: Zu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019271:Hypoxanthine / Q000737:chemistry; D007865:Leeches / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D019271:Hypoxanthine