Genotypic analysis on the ORF-K1 gene of human herpesvirus 8 from patients with Kaposi's sarcoma in Xinjiang, China.
Human herpesvirus 8 (HHV-8) is thought to be essential for the development of all forms of Kaposi's sarcoma (KS). HHV-8 DNA is present virtually in all KS tumor biopsy samples. Genes at both ends of the HHV-8 genome have been shown to vary considerably. Seven major molecular subtypes of HHV-8 were defined based on the amino acid sequence of the open reading frame K1 (ORF-K1), generally known as A, B, C, D, E, F, and Z. Most strains collected worldwide were clustered into two subtypes (A and C). Here, the K1/VR1 region of HHV-8 was amplified by nested PCR in 22 (81.48%) of 27 cases from Xinjiang Uygur Autonomous Region, a province in northwestern China. Phylogenetic analysis on the basis of the K1/VR1 amino acid sequence indicated that the majority of these KS patients were infected by subtype C HHV-8 (n = 18, including 15 belonging to the C2 group), and several by subtype A (n = 4, including 3 being the A1 group). This is the first report of subtype A HHV-8 in China. Furthermore, the correlations between different forms and lesions of KS and different subtypes of HHV-8 were analyzed. The findings showed that subtype A HHV-8 resulted in significantly more frequent mucosal KS lesions than subtype C. However, there was no obvious correlation between different forms of KS and different subtypes of HHV-8.
Zhang
Dezhi
D
Department of Dermatology, The People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China.
Pu
Xiongming
X
Wu
Weidong
W
Jin
Ying
Y
Juhear
Mijiti
M
Wu
Xiujuan
X
D000595:Amino Acid Sequence; D044466:Asian People / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D019288:Herpesvirus 8, Human / Q000145:classification / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012514:Sarcoma, Kaposi / Q000473:pathology / Q000821:virology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*; D014764:Viral Proteins
D004279:DNA, Viral; D008565:Membrane Proteins; D014759:Viral Envelope Proteins; C111600:K1 protein, Human herpesvirus 8; D014764:Viral Proteins