title: Tumor infection by oncolytic reovirus primes adaptive antitumor immunity.
abstract: OBJECTIVE
  Early clinical trials are under way exploring the direct oncolytic potential of reovirus. This study addresses whether tumor infection by reovirus is also able to generate bystander, adaptive antitumor immunity.
  
  
  METHODS
  Reovirus was delivered intravenously to C57BL/6 mice bearing lymph node metastases from the murine melanoma, B16-tk, with assessment of nodal metastatic clearance, priming of antitumor immunity against the tumor-associated antigen tyrosinase-related protein-2, and cytokine responses. In an in vitro human system, the effect of reovirus infection on the ability of Mel888 melanoma cells to activate and load dendritic cells for cytotoxic lymphocyte (CTL) priming was investigated.
  
  
  RESULTS
  In the murine model, a single intravenous dose of reovirus reduced metastatic lymph node burden and induced antitumor immunity (splenocyte response to tyrosinase-related protein-2 and interleukin-12 production in disaggregated lymph nodes). In vitro human assays revealed that uninfected Mel888 cells failed to induce dendritic cell maturation or support priming of an anti-Mel888 CTL response. In contrast, reovirus-infected Mel888 cells (reo-Mel) matured dendritic cells in a reovirus dose-dependent manner. When cultured with autologous peripheral blood lymphocytes, dendritic cells loaded with reo-Mel induced lymphocyte expansion, IFN-gamma production, specific anti-Mel888 cell cytotoxicity, and cross-primed CD8+ T cells specific against the human tumor-associated antigen MART-1.
  
  
  CONCLUSIONS
  Reovirus infection of tumor cells reduces metastatic disease burden and primes antitumor immunity. Future clinical trials should be designed to explore both direct cytotoxic and immunotherapeutic effects of reovirus.
authors.affiliation: Cancer Research UK Clinical Centre, Leeds Institute of Molecular Medicine, University of Leeds, Leeds, United Kingdom.
authors.forename: Robin J
authors.initials: RJ
authors.lastname: Prestwich
authors.forename: Fiona
authors.initials: F
authors.lastname: Errington
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Ilett
authors.forename: Ruth S M
authors.initials: RS
authors.lastname: Morgan
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Scott
authors.forename: Timothy
authors.initials: T
authors.lastname: Kottke
authors.forename: Jill
authors.initials: J
authors.lastname: Thompson
authors.forename: Ewan E
authors.initials: EE
authors.lastname: Morrison
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Harrington
authors.forename: Hardev S
authors.initials: HS
authors.lastname: Pandha
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Selby
authors.forename: Richard G
authors.initials: RG
authors.lastname: Vile
authors.forename: Alan A
authors.initials: AA
authors.lastname: Melcher
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D019746:Intramolecular Oxidoreductases / Q000276:immunology; D008207:Lymphatic Metastasis / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology; D058965:MART-1 Antigen; D012089:Mammalian orthoreovirus 3 / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000276:immunology; D009374:Neoplasms, Experimental / Q000473:pathology / Q000628:therapy* / Q000821:virology; D050130:Oncolytic Virotherapy / Q000379:methods*; D016133:Polymerase Chain Reaction; D012088:Reoviridae Infections / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D058965:MART-1 Antigen; C548667:MLANA protein, human; C548668:Mlana protein, mouse; D009363:Neoplasm Proteins; D007371:Interferon-gamma; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase