Improving the capillary electrophoretic analysis of poliovirus using a Plackett-Burman design.
Separation techniques may offer interesting alternatives to classical virological techniques both for fundamental research purposes and for vaccine manufacturing. A capillary electrophoretic method for the analysis of the poliovirus was developed based on conditions for the human rhinovirus taken from literature. The method was optimized using a 12-experiment Plackett-Burman design, applied in order to examine simultaneously the effects of eight factors on responses such as, mobility of the electroosmotic flow, effective mobility of the poliovirus, analysis time and resolution between the virus peak and a system peak. The proposed method manages to perform an acceptable separation of poliovirus particles using a 50 mM borate buffer with 25 mM SDS, in an uncoated fused-silica capillary upon application of 10 kV at 30 degrees C. The linearity of the proposed method was investigated for a range of poliovirus dilutions up to 140 microg/mL.
Oita
Iuliana
I
Department of Analytical Chemistry and Pharmaceutical Technology, Pharmaceutical Institute, Vrije Universiteit Brussel - VUB, Laarbeeklaan 103, 1090 Brussels, Belgium.
Halewyck
Hadewych
H
Pieters
Sigrid
S
Dejaegher
Bieke
B
Thys
Bert
B
Rombaut
Bart
B
Heyden
Yvan Vander
YV
D001881:Borates; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D016014:Linear Models; D017955:Poliovirus / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013997:Time Factors
D001881:Borates; D002021:Buffers; D012822:Silicon Dioxide