title: Production of high titre disabled infectious single cycle (DISC) HSV from a microcarrier culture.
abstract: Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactors. The cells are infected at an MOI of 0.01 pfu cell-1 and the culture harvested 60-72 h later. The infected cells are removed from the microcarriers by the addition of a hypotonic saline and the virus released by low-pressure disruption techniques. Virus titres achieved are compared to the standard roller bottle process. The resulting material is the starting point for the purification of the DISC-HSV virus.
authors.affiliation: Cantab Pharmaceuticals Research Ltd., 310 Cambridge Science Park, Cambridge, CB4 0WG, UK.
authors.forename: T A
authors.initials: TA
authors.lastname: Zecchini
authors.forename: R J
authors.initials: RJ
authors.lastname: Smith