Carbon dioxide does not affect the methylation status of prognostic important oncogenes Rassf1A and DCR2 in neuroblastoma cells.
OBJECTIVE
The aim of the current study was to investigate effects of CO(2) atmosphere, mimicking conditions of the pneumoperitoneum during laparoscopy, on epigenetic conditions of Rassf1A and DCR2 oncogenes in neuroblastoma cells.


METHODS
SH-SY5Y neuroblastoma cells were exposed to 100% CO(2) for 4 h. Cells were lysed 4, 8 and 168 h after exposure. After methylation analysis of Rassf1A and DCR2 with polymerase chain reaction, results were compared to those of physiologically incubated neuroblastoma cells.


RESULTS
No significant changes were found after exposure to carbon dioxide compared to the control. Values of methylated Rassf1A were 12.6 +/- 1.1 versus 13.2 +/- 1.4 ng/microl in the controls, respectively (4 h after incubation), 12.6 +/- 1.2 versus 15.1 +/- 0.9 ng/microl (8 h) and 14.2 +/- 1.5 versus 11.7 +/- 1.3 ng/microl (168 h). DCR2 showed values of 4.6 +/- 0.5 versus 3.7 +/- 0.5 ng/microl (4 h), 3.8 +/- 0.5 versus 4.1 +/- 0.4 ng/microl (8 h) and 3.6 +/- 0.4 versus 3.8 +/- 0.5 ng/microl (168 h).


CONCLUSIONS
Exposure of neuroblastoma cells to 100% CO(2) does not alter methylation of two prognostic relevant index genes. It seems therefore unlikely that effects on methylation levels within CO(2) pneumoperitoneum lead to epigenetic changes in neuroblastoma.
Reismann
Marc
M
Department of Pediatric Surgery, Hannover Medical School, 30625, Hannover, Germany. reismann.marc@mh-hannover.de
Lehmann
Daniel
D
Quandte
Matthias
M
Ure
Benno M
BM
Glüer
Sylvia
S
D002245:Carbon Dioxide / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019175:DNA Methylation / Q000187:drug effects*; D005740:Gases / Q000494:pharmacology*; D006801:Humans; D009447:Neuroblastoma; D009857:Oncogenes; D011379:Prognosis; D053319:Tumor Necrosis Factor Decoy Receptors / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
D005740:Gases; C411762:RASSF1 protein, human; C451501:TNFRSF10D protein, human; D053319:Tumor Necrosis Factor Decoy Receptors; D025521:Tumor Suppressor Proteins; D002245:Carbon Dioxide