Changes in optical properties of tissue during acute wound healing in an animal model.
Changes of optical properties of wound tissue in hairless rats were quantified by diffuse photon density wave methodology at near-infrared frequencies. The diffusion equation for semi-infinite media was used to calculate the absorption and scattering coefficients based on measurements of phase and amplitude with a frequency domain device. There was an increase in the absorption and scattering coefficients and a decrease in blood saturation of the wounds compared with the nonwounded sites. The changes correlated with the healing stage of the wound. The data obtained were supported by immunohistochemical analysis of wound tissue. These results verified now by two independent animal studies could suggest a noninvasive method to detect the progress of wound healing.
Papazoglou
Elisabeth S
ES
Drexel University, School of Biomedical Engineering, Philadelphia, Pennsylvania 19104, USA. elisabeth.s.papazoglou@drexel.edu
Weingarten
Michael S
MS
Zubkov
Leonid
L
Neidrauer
Michael
M
Zhu
Linda
L
Tyagi
Som
S
Pourrezaei
Kambiz
K
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008027:Light; D051381:Rats; D054772:Rats, Hairless; D017207:Rats, Sprague-Dawley; D012031:Refractometry / Q000379:methods*; D012542:Scattering, Radiation; D012867:Skin / Q000293:injuries* / Q000503:physiopathology*; D013057:Spectrum Analysis / Q000379:methods*; D014945:Wound Healing / Q000502:physiology*; D014950:Wounds, Penetrating / Q000503:physiopathology*