title: Over-expression of protein kinase Balpha enhances recombinant protein expression in transient systems.
abstract: As more genes are being identified through genomic techniques,the need to rapidly express recombinant proteins for functionalstudies has become increasingly acute. Transient expression ofrecombinant protein using COS-1, CV-1 and 293 cells is widelyused to address this need. To improve the robustness of hostcells for transient expression, the effect of over-expression ofProtein Kinase Balpha has been explored. In this report wedemonstrate that over-expression of Protein Kinase Balpha canimprove transient recombinant protein expression 40% to >200%depending on the protein being expressed and the cell line used.
authors.affiliation: Department of Cell Sciences, Immunex Corporation, 51 University St, Seattle, WA, 98101, USA.
authors.forename: Elham
authors.initials: E
authors.lastname: Ettehadieh
authors.forename: Sharon
authors.initials: S
authors.lastname: Wong-Madden
authors.forename: Teri
authors.initials: T
authors.lastname: Aldrich
authors.forename: Kelly
authors.initials: K
authors.lastname: Lane
authors.forename: Arvia E
authors.initials: AE
authors.lastname: Morris