title: Sweet silver: a formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry.
abstract: Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features, but its compatibility with MS is limited. We describe here, a new variant of silver staining that is completely formaldehyde-free. Reducing sugars in alkaline borate buffer are used as developers. While keeping the benefits of silver staining, this method is shown to afford a much better performance in terms of compatibility with MS, both in PMF by MALDI and in LC/ESI/MS/MS.
authors.affiliation: CEA-Laboratoire de Biochimie et Biophysique des systèmes Intégrés, iRTSV/BBSI, CEA-Grenoble, Grenoble, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Chevallet
authors.forename: Sylvie
authors.initials: S
authors.lastname: Luche
authors.forename: Hélène
authors.initials: H
authors.lastname: Diemer
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Strub
authors.forename: Alain
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: Thierry
authors.initials: T
authors.lastname: Rabilloud
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D005557:Formaldehyde / Q000737:chemistry*; D008291:Malate Dehydrogenase / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D016622:Silver Staining / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016596:Vinculin / Q000032:analysis / Q000737:chemistry
chemical_list: D000447:Aldehydes; D016596:Vinculin; D005557:Formaldehyde; D008291:Malate Dehydrogenase