title: Odorant receptor (OR) gene choice is biased and non-clonal in two olfactory placode cell lines, and OR RNA is nuclear prior to differentiation of these lines.
abstract: We have investigated two clonal mouse olfactory placode (OP) cell lines as a model system for studying endogenous odorant receptor (OR) regulation. Both lines can be differentiated into bipolar neurons with transcriptional profiles consistent with mature sensory neurons. We show that single cells exhibit monogenic OR expression like sensory neurons in vivo. Monogenic OR expression is established in undifferentiated cells and persists through differentiation, but OR gene choice is not a clonal property of either cell line. Interestingly, OR RNA shifts from predominantly nuclear to cytoplasma during differentiation of both cell lines. Finally, our data indicate that a restricted subset of OR genes and OR clusters are over-represented in cell populations, suggesting either a pre-existing intrinsic bias in OP founder cells or extrinsic influences arising from culture conditions.
authors.affiliation: Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459, USA.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Pathak
authors.forename: Paul
authors.initials: P
authors.lastname: Johnson
authors.forename: Michael
authors.initials: M
authors.lastname: Getman
authors.forename: Robert P
authors.initials: RP
authors.lastname: Lane
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004625:Embryo, Nonmammalian; D019922:GAP-43 Protein / Q000235:genetics / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D051842:Olfactory Marker Protein / Q000235:genetics / Q000378:metabolism; D009831:Olfactory Mucosa / Q000196:embryology / Q000378:metabolism; D018034:Olfactory Receptor Neurons / Q000166:cytology*; D012333:RNA, Messenger / Q000378:metabolism*; D018035:Receptors, Odorant / Q000145:classification / Q000235:genetics*
chemical_list: D000199:Actins; D019922:GAP-43 Protein; D051842:Olfactory Marker Protein; C497598:Omp protein, mouse; D012333:RNA, Messenger; D018035:Receptors, Odorant; D019204:GTP-Binding Proteins