title: CD3-positive large B-cell lymphoma.
abstract: It is not uncommon for some B-lineage non-Hodgkin lymphomas (NHLs) to aberrantly coexpress T-cell markers, particularly CD5, as well as CD7, CD2, CD4, and/or CD8 in rare cases. Cases of CD3-positive B-cell NHL, however, have not previously been described in the literature. We present 4 cases of large B-cell lymphoma aberrantly coexpressing T-cell marker CD3 and B-lineage markers as well as demonstrating clonal rearrangement of the immunoglobulin genes but not the gamma T-cell receptor gene. To our knowledge, this represents the first series report of B-cell NHL coexpressing T-lineage-specific marker CD3. The identification of such cases indicates that the use of CD3 antibody alone in paraffin sections may lead to an incorrect determination of cell lineage in some B-cell NHL. Immunohistochemistry using additional cell lineage specific markers or molecular analysis for antigen receptor gene rearrangements are necessary for correct determination of the cell lineage in such cases.
authors.affiliation: Department of Pathology and Laboratory Medicine, Loma Linda University Medical Center, Loma Linda, CA, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Sean
authors.initials: S
authors.lastname: Lau
authors.forename: Ravi I
authors.initials: RI
authors.lastname: Raghavan
authors.forename: Edward H
authors.initials: EH
authors.lastname: Rowsell
authors.forename: Jonathan
authors.initials: J
authors.lastname: Said
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: Weiss
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000328:Adult; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000737:chemistry / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D017252:CD3 Complex / Q000032:analysis*; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D017403:In Situ Hybridization; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D016601:RNA-Binding Proteins / Q000032:analysis; D012269:Ribosomal Proteins / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D017252:CD3 Complex; D004273:DNA, Neoplasm; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; C067169:RPL22 protein, human