title: Inhibition effect of pcDNA-tum-5 on the growth of S180 tumor.
abstract: Tumor growth and metastasis depend on vessel formation, and inhibition of angiogenesis of tumor by production of anti-angiogenic drugs should be a promising approach for cancer therapy. Tumstatin is an angiogenesis inhibitor. The anti-angiogenic activity of tumstatin is localized to the 54-132 amino acids. The gene fragment encoding amino acids 45-132 of tumstatin (tum-5) was subcloned into pcDNA3.1 (pcDNA-tum5). Tum-5 protein could be expressed and secreted in CHO cells after transfection. The conditioned medium (containing tum-5 protein) from the transfectant has an anti-angiogenic effect on HUVEC cells in vitro. The anti-tumor effect of pcDNA-tum5 on mice bearing S180 tumors was evaluated. The results showed that pcDNA-tum-5 has a significant inhibition activity in the growth of the tumors. This study suggests that the gene delivery of tum-5 may be an effective strategy for cancer therapy.
authors.affiliation: Biotechnology Center of The Fourth Military Medical University, 17 Changle West Road, 710032, Xi'an, People's Republic of China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: You
authors.forename: Xiaochang
authors.initials: X
authors.lastname: Xue
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Qin
authors.forename: Cun
authors.initials: C
authors.lastname: Zhang
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.forename: Changli
authors.initials: C
authors.lastname: Giang
authors.forename: Shouzhen
authors.initials: S
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Wenhua
authors.initials: W
authors.lastname: Zhu
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Ran
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Yingqi
authors.initials: Y
authors.lastname: Zhang