title: [Application of multicolor fluorescence in situ hybridization to early diagnosis of esophageal squamous cell carcinoma].
abstract: OBJECTIVE
  Detection of chromosome aberrations has been applied to diagnose some tumors. However, there are no chromosomal markers for the diagnosis of esophageal carcinoma so far. This study was to analyze aberrations of some chromosomes in esophageal squamous cell carcinoma (ESCC) and its premalignant lesions, thus to explore the application of multicolor fluorescence in situ hybridization (M-FISH) for the early diagnosis and risk prediction of precursor lesions of ESCC.
  
  
  METHODS
  Aberration statuses of chromosomes 3, 8, 10, 12, 17 and 20 were investigated in 124 esophageal tissue samples from 113 patients using M-FISH with chromosome-specific centromere DNA probes. The relationship between chromosome gains and clinicopathologic parameters was analyzed.
  
  
  RESULTS
  Copy number gains on chromosomes 3, 8, 10, 12, 17 and 20 in ESCC were 80.9% (93/115), 81.0% (94/116), 70.5% (79/112), 75.9% (85/112), 68.7% (79/115) and 82.8% (48/58), respectively. No statistical relations were found between chromosome aberrations and clinicopathologic parameters (P>0.05). Polysomy rates of the six chromosomes in precursor lesions were 62.5% (5/8), 75.0% (6/8), 62.5% (5/8), 87.5% (7/8), 87.5% (7/8) and 100% (3/3); while those in early-stage ESCC were 80.0% (12/15), 93.8% (15/16), 71.4% (10/14), 64.3% (9/14), 75.0% (12/16) and 63.6% (7/11).
  
  
  CONCLUSIONS
  Positive aneuploidy rates of chromosomes 3,8,10,12,17 and 20 are highly detected in both ESCC and its precursor lesions. M-FISH is helpful in the early diagnosis of ESCC, thus it may be used as a method to predict the risk to ESCC.
authors.affiliation: Department of Endoscopy, Cancer Institute / Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Yao
authors.forename: Shun
authors.initials: S
authors.lastname: He
authors.forename: Yu-Peng
authors.initials: YP
authors.lastname: Wu
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Wang
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Han
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.forename: Gui-Qi
authors.initials: GQ
authors.lastname: Wang
authors.forename: Ming-Rong
authors.initials: MR
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002879:Chromosomes, Human, Pair 10; D002881:Chromosomes, Human, Pair 12; D002886:Chromosomes, Human, Pair 17; D002890:Chromosomes, Human, Pair 20; D002893:Chromosomes, Human, Pair 3; D002898:Chromosomes, Human, Pair 8; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology