Transcription factor Yin Yang 1 represses fetal troponin I gene expression in neonatal myocardial cells.
Yin Yang 1 (YY1) is a transcription factor that can activate or repress expression of a variety of genes and is involved in several developmental processes. While some transcription factors are known to modulate skeletal myogenesis, the regulation of fetal troponin I (ssTnI) expression by YY1 in cardiac development has not been studied. The present study shows that the fetal troponin I gene expression in neonatal myocardium was reduced by overexpression of YY1, while cardiac troponin I (cTnI) did not show any significant decrease. And a dose-response inhibition by YY1 was observed in fetal troponin I promoter induced transcriptional activities. Mutation of YY1-binding site can abolish the inhibitory effect and YY1 silencing in neonatal myocardium resulted in an increase of ssTnI protein expression. Our results indicate that YY1 is a novel regulator of fetal TnI transcription in the heart.
Nan
Changlong
C
College of Biomedical Science, Center for Molecular and Biology and Biotechnology, Department of Biomedical Sciences, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431, USA.
Huang
Xupei
X
D000595:Amino Acid Sequence; D000818:Animals; D015536:Down-Regulation; D005333:Fetus / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D051379:Mice; D008969:Molecular Sequence Data; D032383:Myocytes, Cardiac / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D019210:Troponin I / Q000235:genetics*; D050986:YY1 Transcription Factor / Q000235:genetics / Q000378:metabolism*
D019210:Troponin I; D050986:YY1 Transcription Factor; C494699:Yy1 protein, mouse