Magnetic beads based immunoaffinity capillary electrophoresis of total serum IgE with laser-induced fluorescence detection.
A magnetic beads based immunoaffinity capillary electrophoresis method for total Immunoglobulin E quantification in serum has been developed. The method combines speed, automation ability, and minimal sample consumption. Only 1 microL of serum is required while the whole immunoaffinity capillary electrophoresis method is performed in less than 50 min. The concomitant use of online immunocapture, transient isotachophoresis, and laser-induced fluorescence detection provides a sensitivity in the low picomolar range and a highly linear fluorescence response over 4 orders of magnitude (IgE concentration ranging from 2.4 to 2400 ng/mL). After validation with a reference material, the method has been successfully applied to the quantification of total IgEs in patient sera. The results compared well with classical ImmunoCap data.
Chen
Hong-Xu
HX
Beijing National Laboratory for Molecular Sciences, College of Chemistry, Peking University, Beijing 100871, China.
Busnel
Jean-Marc
JM
Peltre
Gabriel
G
Zhang
Xin-Xiang
XX
Girault
Hubert H
HH
D002846:Chromatography, Affinity; D019075:Electrophoresis, Capillary; D005240:Feasibility Studies; D005453:Fluorescence; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007073:Immunoglobulin E / Q000097:blood*; D007834:Lasers; D008280:Magnetics
D007073:Immunoglobulin E