title: Controlled remineralization of enamel in the presence of amelogenin and fluoride.
abstract: Reconstructing enamel-like structures on teeth have been an important topic of study in the material sciences and dentistry. The important role of amelogenin in modulating the mineralization of organized calcium phosphate crystals has been previously reported. We used amelogenin and utilized a modified biomimetic deposition method to remineralize the surface of etched enamel to form mineral layers containing organized needle-like fluoridated hydroxyapatite crystals. The effect of a recombinant amelogenins (rP172) on the microstructure of the mineral in the coating was analyzed by SEM, XRD and FT-IR. At rP172 concentrations below 33 microg/mL, no significant effect was observed. In the presence of 1 mg/L F and at a concentration of 33 microg/mL rP172, formation of fused crystals growing from the enamel surface was initiated. Amelogenin promoted the oriented bundle formation of needle-like fluoridated hydroxyapatite in a dose dependent manner. Biomimetic synthesis of the amelogenin fluoridated hydroxyapatite nano-composite is one of the primary steps towards the development and design of novel biomaterial for future application in reparative and restorative dentistry.
authors.affiliation: Center for Craniofacial Molecular Biology, University of Southern California, 2250 Alcazar Street, CSA 103, HSC, Los Angeles, CA 90033, USA.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Fan
authors.forename: Zhi
authors.initials: Z
authors.lastname: Sun
authors.forename: Janet
authors.initials: J
authors.lastname: Moradian-Oldak
mesh_terms: D053523:Amelogenin / Q000494:pharmacology*; D000818:Animals; D002130:Calcium Phosphates / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000378:metabolism; D003460:Crystallization; D003743:Dental Enamel / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D005459:Fluorides / Q000494:pharmacology*; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine; D014089:Tooth Remineralization; D014961:X-Ray Diffraction
chemical_list: D053523:Amelogenin; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; C020243:calcium phosphate; D005459:Fluorides