title: Utilization of fermented barley extract obtained from a by-product of barley shochu for nisin production.
abstract: Fermented barley extract (FBE) obtained from a barley shochu by-product (shochu kasu) and its ethanol fractions were evaluated as a medium and supplement, respectively, for nisin A production by Lactococcus lactis subsp. lactis ATCC 11454. A Brix 2.5 FBE medium supplemented with glucose provided a high level of nisin A production with a nisin yield comparable to that from a nutritionally rich laboratory medium (basal medium). By adding the ethanol-insoluble (EI) fraction of FBE to the basal medium, nisin A production was enhanced concomitant with an increase in bacterial cell growth, while the ethanol-soluble (ES) fraction had a negative effect on nisin A production. These findings indicate that FBE obtained from shochu kasu can be utilized as a preferable medium for nisin A production and could be converted into a value-added food product having preservative functions. The procedure developed in this study would promote recycling of shochu kasu.
authors.affiliation: Research Laboratory, Sanwa Shurui, Co. Ltd., 2231-1 Yamamoto, Usa, Oita 879-0495, Japan. furuta-y@kokuzo.co.jp
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Furuta
authors.forename: Naruyuki
authors.initials: N
authors.lastname: Maruoka
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nakamura
authors.forename: Toshiro
authors.initials: T
authors.lastname: Omori
authors.forename: Kenji
authors.initials: K
authors.lastname: Sonomoto
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D001467:Hordeum / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D013294:Lactococcus lactis / Q000378:metabolism; D009561:Nisin / Q000096:biosynthesis* / Q000737:chemistry; D012995:Solubility; D013696:Temperature
chemical_list: D003470:Culture Media; D007773:Lactates; D009561:Nisin; D019344:Lactic Acid; D000431:Ethanol; C102526:nisin A; D005947:Glucose