Isolation and confirmation of Yersinia pestis mutants exempt from select agent regulations.
This unit describes protocols for Yersinia pestis to confirm plasmid profiles, construct and confirm a Deltapgm mutation, and cure the low-calcium response (Lcr) plasmid encoding a type III secretion system (TTSS). Strains lacking either the chromosomal pgm locus or the Lcr plasmid can be safely studied under BSL-2 conditions and are exempt from Select Agent regulations in the U.S.
Perry
Robert D
RD
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky, USA.
Bearden
Scott W
SW
D003264:Containment of Biohazards / Q000379:methods; D017353:Gene Deletion; D010957:Plasmids; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*; D015010:Yersinia pestis / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*
D037521:Virulence Factors