title: Use of liquid chromatography with electrochemical detection for the determination of antioxidants in less common fruits.
abstract: Neurodegenerative disorders (NDD) have become the common global health burden over the last several decades. According to World Health Organization (WHO), a staggering 30 million people will be affected by Alzheimer's disease in Europe and the USA by 2050. Effective therapies in this complex field considering the multitude of symptoms associated with NDD indications, have not been found yet. Based on the results of NDD related studies, prevention appears to be the promise alternative. Antioxidative and anti-inflammatory properties are hypothesized for natural phenolics, a group of plant secondary products that may positively impact neurodegenerative diseases. In these studies, phenolic-rich extracts from less common fruit species: Blue honeysuckle (Lonicera edulis, Turcz. ex. Freyn), Saskatoon berry (Amelanchier alnifolia Nutt.), and Chinese hawthorn (Crateagus pinnatifida Bunge) were obtained and analyzed to detect neuroprotective substances content and establish a potential therapeutic value. High performance liquid chromatography with electrochemical detection was optimized and further applied on analysis of the extracts of less common fruit species. It was observed that Chinese hawthorn and Blue honeysuckle extracts are potent source of neuroprotective phenolic antioxidants. In accordance the results, it appears that the fruit or formulated products may have the potential for the prevention of neurodegenerative diseases.
authors.affiliation: Department of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel University of Agriculture and Forestry, Lednice, Czech Republic. xgazdik@node.mendelu.cz
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Gazdik
authors.forename: Vojtech
authors.initials: V
authors.lastname: Reznicek
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.forename: Ondrej
authors.initials: O
authors.lastname: Zitka
authors.forename: Tunde
authors.initials: T
authors.lastname: Jurikova
authors.forename: Boris
authors.initials: B
authors.lastname: Krska
authors.forename: Jan
authors.initials: J
authors.lastname: Matuskovic
authors.forename: Jan
authors.initials: J
authors.lastname: Plsek
authors.forename: Jan
authors.initials: J
authors.lastname: Saloun
authors.forename: Ales
authors.initials: A
authors.lastname: Horna
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D010129:4-Aminobenzoic Acid / Q000032:analysis; D000975:Antioxidants / Q000032:analysis* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D027825:Crataegus / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation; D005638:Fruit / Q000737:chemistry*; D005707:Gallic Acid / Q000032:analysis; D031239:Lonicera / Q000737:chemistry; D019636:Neurodegenerative Diseases / Q000188:drug therapy / Q000517:prevention & control; D011794:Quercetin / Q000032:analysis; D027824:Rosaceae / Q000737:chemistry; D012431:Rutin / Q000032:analysis
chemical_list: D000975:Antioxidants; D012431:Rutin; D005707:Gallic Acid; D011794:Quercetin; D010129:4-Aminobenzoic Acid