Asymmetric synthesis of (S)-alpha-methylbenzylamine by recombinant Escherichia coli co-expressing omega-transaminase and acetolactate synthase.
To produce (S)-alpha-methylbenzylamine (MBA) from acetophenone, recombinant Escherichia coli co-expressing omega-transaminase and acetolactate synthase was used as a whole-cell biocatalyst. The solvent-bridge reaction system increased the yield of the whole-cell reaction by 2.5-fold, and the inhibitory (S)-alpha-MBA produced in the omega-transaminase reaction solution (pH 8.0) moved into the extraction solution (pH 3.0) via an organic solvent.
Yun
Hyungdon
H
School of Biotechnology, Yeungnam University, Gyeongbuk, Korea.
Kim
Byung-Gee
BG
D000095:Acetolactate Synthase / Q000378:metabolism*; D055162:Biocatalysis; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D010627:Phenethylamines / Q000737:chemistry / Q000378:metabolism*; D012997:Solvents; D013237:Stereoisomerism; D000637:Transaminases / Q000378:metabolism*
D004274:DNA, Recombinant; D010627:Phenethylamines; D012997:Solvents; D000095:Acetolactate Synthase; D000637:Transaminases; C033198:1-phenethylamine