Human immunodeficiency virus type 1 chronic infection is associated with different gene expression in MT-4, H9 and U937 cell lines.
To investigate cellular factors involved in HIV-1 chronic infection, three cell lines chronically infected with the same HIV-1 viral isolate (s61) were studied by cDNA microarray analysis. Two T cell lines, H61 and M61, showed the characteristics of a persistent infection whereas U61 cell line displayed a latent infection pattern. Analysis of genes with altered expression in the three cell lines revealed evidence of apoptosis control by up-regulation of anti-apoptotic genes and down-regulation of pro-apoptotic genes. In addition, cell cycle control was affected in the two persistent T cell lines particularly through the down-regulation of cyclin-dependent kinase inhibitor 1A (CDKN1A/p21). Moreover, each cell line showed specific characteristics, like in M61 cells, genes related with cellular activation and with cell migration and motility. In U61 cells, genes associated with immune response were activated. Genes with altered expression in our experiments, and not previously related with HIV such as ANXA 1 or CFLAR were detected and validated. This work revealed that different cell mechanism such as control of apoptosis and cell cycle are important for "in vitro" HIV-1 chronic infections, and discovered new genes previously not related with HIV-1 replication.
Olivares
Isabel
I
Servicio de Virología Molecular, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera de Pozuelo Km 2, Majadahonda, 28220 Madrid, Spain.
Ballester
Alicia
A
Lombardia
Luis
L
Dominguez
Orlando
O
López-Galíndez
Cecilio
C
D045744:Cell Line, Tumor; D002908:Chronic Disease; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D015658:HIV Infections / Q000235:genetics / Q000503:physiopathology*; D015497:HIV-1 / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D020298:U937 Cells; D014775:Virus Activation / Q000235:genetics / Q000502:physiology*; D017735:Virus Latency / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
D012367:RNA, Viral