Immunomodulatory activity of isoflavones isolated from Iris germanica (Iridaceae) on T-lymphocytes and cytokines.
The immunomodulatory activities of two isoflavones, 5,7-dihydroxy-6,4'-dimethoxyisoflavone (irisolidone) (1) and 5,4'-dihydroxy-6,7-methylenedioxyisoflavone (irilone) (2) isolated from Iris germanica (Iridaceae) is reported. Their influence on production of T-lymphocytes (CD4+ and CD8+ cells) and T-cell cytokines, namely Th1: IL-2, IFN-gamma and Th2: IL-4 and IL-5 in a dose-dependent manner was studied by flow cytometric method in Balb/c mice. Oral administration of drugs at doses of 0.1-0.8 mg/kg per oral dose showed 1 to possess stimulatory activity on T-cells and Th1 cytokine production, while as 2 acted as an immunosuppressant for both cells and cytokines. The methylated products of 1 and 2 showed a similar trend to that of their parent compounds but their activity was drastically decreased revealing the importance of free phenolic groups for their immunomodulating activities.
Nazir
Nighat
N
Department of Chemistry, University of Kashmir, Hazratbal, Srinagar-190006, J&K, India. nighat052000@yahoo.com.au
Koul
Surrinder
S
Qurishi
Mushtaq Ahmad
MA
Taneja
Subhash Chander
SC
Ahmad
Sheikh Fayaz
SF
Khan
Beenish
B
Bani
Sarang
S
Qazi
Ghulam Nabi
GN
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D016207:Cytokines / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D005419:Flavonoids / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000276:immunology / Q000494:pharmacology; D031323:Iris Plant / Q000737:chemistry*; D007529:Isoflavones / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000276:immunology / Q000494:pharmacology; D012756:Sheep; D013601:T-Lymphocytes / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D016207:Cytokines; D005419:Flavonoids; D007166:Immunosuppressive Agents; D007529:Isoflavones; D010936:Plant Extracts; C530234:irilone; C423902:irisolidone