HCV replicons: overview and basic protocols.
Subgenomic replicons have been the first efficient cell-culture system for HCV and still are a valuable tool for studying different aspects of RNA replication. A variety of replicons based on different viral isolates and vector designs have been established. Here, I give a brief overview of viral isolates, applicable host-cell lines, replicon structures, and general considerations regarding replicon experiments, supplemented by basic protocols for in vitro transcription, electroporation, selection of replicon cells, transient replication assays, and northern hybridization.
Lohmann
Volker
V
University of Heidelberg, Heidelberg, Germany.
D000818:Animals; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D024882:Drug Resistance, Viral; D018274:Electroporation; D017930:Genes, Reporter / Q000235:genetics; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D012093:Replicon / Q000235:genetics*; D014158:Transcription, Genetic; D014779:Virus Replication / Q000235:genetics*
D012367:RNA, Viral; D008156:Luciferases