[Protective effect of Huangban Granule against light-induced retinal damage in rats].
OBJECTIVE
To observe the protective effect of Huangban Granule, a compound of traditional Chinese herbal medicine, on rats with retinal damage induced by light.


METHODS
A total of 24 male Sprague-Dawley (SD) rats were randomly divided into normal control group, untreated group and Huangban Granule group. Retinal light damage was induced by exposure to constant white fluorescent light for 5 hours at an illumination of 2,800 Lux. The Huangban Granule was given 10 days before light exposure until the animals were sacrificed in Huangban Granule group, and an equal volume of distilled water for the rats in untreated group. Electroretinogram (ERG) was recorded in all animals 2 weeks after light exposure and the animals were sacrificed for histopathological examination of retina. The outer nuclear layers (ONLs) on the superior and inferior retina were counted.


RESULTS
Fourteen days after light exposure, the ONLs on the superior retina were 3 to 6 in the untreated group and 7 to 9 in treatment group. There were 9 to 11 layers in normal group. The mean number of ONLs in the untreated group (4.68+/-1.64) was less than that in the treatment group (8.23+/-1.35) (P<0.01). B-wave amplitudes were (319.38+/-71.96) muV and (135.16+/-42.30) muV in Huangban Granule group and the untreated group respectively (P<0.01). A-wave amplitudes were (184.63+/-47.23) muV and (83.35+/-27.75) muV (P<0.01), and oscillatory potential amplitudes were (239.38+/-20.19) muV and (125.44+/-26.23) muV (P<0.01) respectively in the two group. There was no significant difference in implicit times of a-wave and b-wave among the three groups.


CONCLUSIONS
Huangban Granule obviously protects both function and morphology of the retina from light-induced retinal damage in rats.
Gong
Yuan-yuan
YY
Department of Ophthalmology, First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China.
Song
Yi
Y
Xie
Zheng-gao
ZG
Wu
Xing-wei
XW
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008027:Light / Q000009:adverse effects*; D008297:Male; D020011:Protective Agents / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D012162:Retinal Degeneration / Q000209:etiology / Q000473:pathology / Q000517:prevention & control
D004365:Drugs, Chinese Herbal; D020011:Protective Agents