A genetic selection system for evolving enantioselectivity of enzymes.
As an alternative to screening in the directed evolution of enantioselective enzymes, a selection system has been implemented for a lipase-catalyzed hydrolytic kinetic resolution of a chiral ester.
Reetz
Manfred T
MT
Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim, Germany. reetz@mpi-muelheim.mpg.de.
Höbenreich
Horst
H
Soni
Pankaj
P
Fernández
Layla
L
D000475:Alkenes / Q000138:chemical synthesis / Q000737:chemistry; D002175:Candida / Q000201:enzymology / Q000254:growth & development; D002384:Catalysis; D019020:Directed Molecular Evolution / Q000379:methods*; D004952:Esters / Q000737:chemistry; D005656:Fungal Proteins; D005990:Glycerol / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015394:Molecular Structure; D013237:Stereoisomerism; D013379:Substrate Specificity
C507843:1,2-O-isopropylidene glycerol; D000475:Alkenes; D004952:Esters; D005656:Fungal Proteins; D008049:Lipase; C431349:lipase B, Candida antarctica; D005990:Glycerol