title: The transcriptional regulator PLZF induces the development of CD44 high memory phenotype T cells.
abstract: Transcriptional pathways controlling the development of CD44(hi) memory phenotype (MP) T cells with "innate-like" functions are not well understood. Here we show that the BTB (bric-a-brac, tramtrack, broad complex) domain-containing protein promyelocytic leukemia zinc finger (PLZF) is expressed in CD44(hi), but not in CD44(lo), CD4(+) T cells. Transgenic expression of PLZF during T cell development and in CD4(+) and CD8(+) T cells induced a T cell intrinsic program leading to an increase in peripheral CD44(hi) MP CD4(+) and CD8(+) T cells and a corresponding decrease of naïve CD44(lo) T cells. The MP CD4(+) and CD8(+) T cells produced IFNgamma upon PMA/ionomycin stimulation, thus showing innate-like function. Changes in the naïve versus memory-like subset distribution were already evident in single-positive thymocytes, indicating PLZF-induced T cell developmental alterations. In addition, CD1d-restricted natural killer T cells in PLZF transgenic mice showed impaired development and were severely reduced in the periphery. Finally, after anti-CD3/CD28 stimulation, CD4(+) transgenic T cells showed reduced IL-2 and IFNgamma production but increased IL-4 secretion as a result of enhanced IL-4 production of the CD44(hi)CD62L(+) subset. Our data indicate that PLZF is a novel regulator of the development of CD44(hi) MP T cells with a characteristic partial innate-like phenotype.
authors.affiliation: Division of Immunobiology, Institute of Immunology, Center for Physiology, Pathophysiology and Immunology, Medical University of Vienna, A-1090 Vienna, Austria.
authors.forename: Julia
authors.initials: J
authors.lastname: Raberger
authors.forename: Alexandra
authors.initials: A
authors.lastname: Schebesta
authors.forename: Shinya
authors.initials: S
authors.lastname: Sakaguchi
authors.forename: Nicole
authors.initials: N
authors.lastname: Boucheron
authors.forename: K Emelie M
authors.initials: KE
authors.lastname: Blomberg
authors.forename: Anna
authors.initials: A
authors.lastname: Berglöf
authors.forename: Thomas
authors.initials: T
authors.lastname: Kolbe
authors.forename: C I Edvard
authors.initials: CI
authors.lastname: Smith
authors.forename: Thomas
authors.initials: T
authors.lastname: Rülicke
authors.forename: Wilfried
authors.initials: W
authors.lastname: Ellmeier
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D018960:Hyaluronan Receptors / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology*; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D019041:L-Selectin / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D055611:Natural Killer T-Cells / Q000166:cytology / Q000276:immunology; D010641:Phenotype; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
chemical_list: D016207:Cytokines; D018960:Hyaluronan Receptors; D051741:Kruppel-Like Transcription Factors; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D011948:Receptors, Antigen, T-Cell; C513267:Zbtb16 protein, mouse; D019041:L-Selectin