Analytical challenges in doping control: Comprehensive two-dimensional gas chromatography with time of flight mass spectrometry, a promising option.
Doping control screening based on the enhanced resolution of comprehensive two-dimensional (2D) gas chromatography hyphenated to time of flight mass spectrometer was investigated. The identification of anabolic agents (clenbuterol, norandrosterone, epimetendiol, two methyltestosterone metabolites and 3'-hydroxystanozolol) contained in a spiked urine sample (2ng/ml) was demonstrated. Special emphasis was given to 3'-hydroxystanozolol, mainly considering the difficulty in its detection. In contrast to conventional GC-MS approaches that must use single-ion monitoring, the GC x GC-TOFMS method enabled the identification of that metabolite through the deconvolution of the full mass spectrum and also resolved the co-eluted peaks of 3'-hydroxystanozolol and an endogenous component.
Silva
A I
AI
LAB DOP LADETEC/Instituto de Química, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Cidade Universitária, Rio de Janeiro, CEP 21941-909, RJ, Brazil.
Pereira
H M G
HM
Casilli
A
A
Conceição
F C
FC
Aquino Neto
F R
FR
D045930:Anabolic Agents / Q000652:urine*; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D013197:Stanozolol / Q000031:analogs & derivatives* / Q000652:urine; D015813:Substance Abuse Detection / Q000379:methods*
D045930:Anabolic Agents; C425489:3'-hydroxystanozolol; D013197:Stanozolol