Trifluoromethyldiazirine: an effective photo-induced cross-linking probe for exploring amyloid formation.
The separative and analytical power of ion mobility spectrometry-mass spectrometry combined with photo-induced cross-linking of site-specifically incorporated trifluoromethyldiazirine provides a powerful approach towards structural characterisation of amyloid fibrils.
Smith
David P
DP
Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, UK LS2 9JT.
Anderson
Jon
J
Plante
Jeffrey
J
Ashcroft
Alison E
AE
Radford
Sheena E
SE
Wilson
Andrew J
AJ
Parker
Martin J
MJ
D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D001389:Azirines / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D008697:Methane / Q000031:analogs & derivatives / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D017434:Protein Structure, Tertiary; D021241:Spectrometry, Mass, Electrospray Ionization; D014466:Ultraviolet Rays
D000682:Amyloid; D016229:Amyloid beta-Peptides; D001389:Azirines; D003432:Cross-Linking Reagents; D010455:Peptides; C543572:trifluoromethyldiazirine-phenylalanine; C030011:carbene; D010649:Phenylalanine; D008697:Methane