A window of environmental dependence is evident in multiple phylogenetically distinct subgroups in the faecal community of piglets.
Microbial colonization in neonates has a profound impact on host development. In pigs, we have observed that a window of environmental dependence occurs during neonatal development. This was evident by the sudden onset of faecal community similarity in cohoused neonatal piglets at 3 weeks of age. This effect is postulated to represent a general change in gut community structure. Here, three phylogenetic groups (Clostridium leptum subgroup, Bacteroides and enterobacteria) that were predicted to have distinct ecological roles were monitored using nested denaturing gradient gel electrophoresis to determine the extent to which this window of environmental dependence was exerted throughout the gut community. Colonization trends were found to be similar for all subgroups despite predicted differences in the functional role and niche, and increased similarity between cohabiting piglets occurred in multiple phylogenetic subgroups. This supports the hypothesis that a distinct phase in neonatal development commences after 2 weeks whereby multiple subcommunities of the gut are strongly influenced by the environment.
Thompson
Claire L
CL
School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, NSW, Australia. cthompson@mmb.usyd.edu.au
Holmes
Andrew J
AJ
D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology*; D001439:Bacteroides / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D044822:Biodiversity; D003013:Clostridium / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D017753:Ecosystem; D004586:Electrophoresis / Q000379:methods; D004755:Enterobacteriaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013552:Swine / Q000382:microbiology*
D004269:DNA, Bacterial