title: Cellular microRNA expression correlates with susceptibility of monocytes/macrophages to HIV-1 infection.
abstract: Although both monocytes and macrophages possess essential requirements for HIV-1 entry, peripheral blood monocytes are infrequently infected with HIV-1 in vivo and in vitro. In contrast, tissue macrophages and monocyte-derived macrophages in vitro are highly susceptible to infection with HIV-1 R5 tropic strains. We investigated intracellular anti-HIV-1 factors that contribute to differential susceptibility of monocytes/macrophages to HIV-1 infection. Freshly isolated monocytes from peripheral blood had significantly higher levels of the anti-HIV-1 microRNAs (miRNA, miRNA-28, miRNA-150, miRNA-223, and miRNA-382) than monocyte-derived macrophages. The suppression of these anti-HIV-1 miRNAs in monocytes facilitates HIV-1 infectivity, whereas increase of the anti-HIV-1 miRNA expression in macrophages inhibited HIV-1 replication. These findings provide compelling and direct evidence at the molecular level to support the notion that intracellular anti-HIV-1 miRNA-mediated innate immunity may have a key role in protecting monocytes/macrophages from HIV-1 infection.
authors.affiliation: Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Ye
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yan-Jian
authors.initials: YJ
authors.lastname: Wang
authors.forename: David S
authors.initials: DS
authors.lastname: Metzger
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Ho
mesh_terms: D004198:Disease Susceptibility / Q000821:virology; D015658:HIV Infections / Q000235:genetics*; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000821:virology*; D035683:MicroRNAs / Q000096:biosynthesis*; D009000:Monocytes / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014779:Virus Replication
chemical_list: D035683:MicroRNAs