The amino-terminal domain of glutamate receptor delta2 triggers presynaptic differentiation.
Glutamate receptor (GluR) delta2 selectively expressed in cerebellar Purkinje cells plays key roles in synapse formation, long-term depression and motor learning. We propose that GluRdelta2 regulates synapse formation by making a physical linkage between the active zone and postsynaptic density. To examine the issue, GluRdelta2-transfected 293T cells were cultured with cerebellar neurons. We found numerous punctate signals for presynaptic markers on the surface of 293T cells expressing GluRdelta2. The presynaptic specializations induced by GluRdelta2 were capable of exo- and endocytosis as indicated by FM1-43 dye labeling. Replacement of the extracellular N-terminal domain (NTD) of GluRdelta2 with that of the AMPA receptor GluRalpha1 abolished the inducing activity. The NTD of GluRdelta2 fused to the immunoglobulin constant region successfully induced the accumulation of presynaptic specializations on the surface of beads bearing the fusion protein. These results suggest that GluRdelta2 triggers presynaptic differentiation by direct interaction with presynaptic components through the NTD.
Uemura
Takeshi
T
Department of Molecular Neurobiology and Pharmacology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Mishina
Masayoshi
M
D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D004705:Endocytosis; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007127:Immunoglobulin Constant Regions; D051379:Mice; D017729:Presynaptic Terminals / Q000502:physiology*; D017434:Protein Structure, Tertiary; D011689:Purkinje Cells / Q000502:physiology* / Q000648:ultrastructure; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D017470:Receptors, Glutamate / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D049452:Green Fluorescent Proteins; D007127:Immunoglobulin Constant Regions; D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; C107913:glutamate receptor delta 2