title: Improved diastolic function after myoblast transplantation in a model of ischemia-infarction.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effect of myoblast transplantation on left ventricular function, perfusion, and scar formation after compromised coronary flow.
  
  
  METHODS
  A coronary vessel with Ameroid-induced stenosis was ligated and skeletal muscle was biopsied for isolation and cultivation of myoblasts. Two weeks after ligation, animals were randomly selected to receive intramyocardial injections of 2 x 10(6) myoblasts or vehicle. Fifteen animals survived the whole study period (n=9 and n=6, respectively). All animals underwent cardiac magnetic resonance imaging (MRI) and angiography pretreatment and four weeks posttreatment.
  
  
  RESULTS
  Peak filling rate of the left ventricle improved in the myoblast group (p=0.0048), but not in the control group. Peak ejection rate and duration of diastole improved only in the myoblast group (p=0.049 and p=0.0039, respectively). Ejection fraction or local thickening did not change. Fibrosis and perfusion were similar in both groups, but more microvessels were present histologically in the myoblast group.
  
  
  CONCLUSIONS
  In this preclinical study, autologous myoblast transplantation improved ischemic heart function via enhanced diastolic filling of the left ventricle.
authors.affiliation: Department of Cardiothoracic Surgery, University of Helsinki Meilahti Hospital, Finland. tommi.patila@hus.fi
authors.forename: Tommi
authors.initials: T
authors.lastname: Pätilä
authors.forename: Tuija
authors.initials: T
authors.lastname: Ikonen
authors.forename: Esko
authors.initials: E
authors.lastname: Kankuri
authors.forename: Aki
authors.initials: A
authors.lastname: Uutela
authors.forename: Jyri
authors.initials: J
authors.lastname: Lommi
authors.forename: Leena
authors.initials: L
authors.lastname: Krogerus
authors.forename: Pertteli
authors.initials: P
authors.lastname: Salmenperä
authors.forename: Josef
authors.initials: J
authors.lastname: Bizik
authors.forename: Kirsi
authors.initials: K
authors.lastname: Lauerma
authors.forename: Ari
authors.initials: A
authors.lastname: Harjula
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017023:Coronary Angiography; D003326:Coronary Circulation; D004195:Disease Models, Animal; D005355:Fibrosis; D008279:Magnetic Resonance Imaging; D008833:Microcirculation; D032448:Myoblasts, Skeletal / Q000637:transplantation*; D009200:Myocardial Contraction; D009203:Myocardial Infarction / Q000209:etiology / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D017202:Myocardial Ischemia / Q000150:complications / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D009206:Myocardium / Q000473:pathology; D013318:Stroke Volume; D013552:Swine; D013997:Time Factors; D014182:Transplantation, Autologous; D016277:Ventricular Function, Left