title: HCV replicons: overview and basic protocols.
abstract: Subgenomic replicons have been the first efficient cell-culture system for HCV and still are a valuable tool for studying different aspects of RNA replication. A variety of replicons based on different viral isolates and vector designs have been established. Here, I give a brief overview of viral isolates, applicable host-cell lines, replicon structures, and general considerations regarding replicon experiments, supplemented by basic protocols for in vitro transcription, electroporation, selection of replicon cells, transient replication assays, and northern hybridization.
authors.affiliation: University of Heidelberg, Heidelberg, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Lohmann
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D024882:Drug Resistance, Viral; D018274:Electroporation; D017930:Genes, Reporter / Q000235:genetics; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D012093:Replicon / Q000235:genetics*; D014158:Transcription, Genetic; D014779:Virus Replication / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D008156:Luciferases