Volume-activated chloride channels contribute to cell-cycle-dependent regulation of HeLa cell migration.
The activation of volume-activated chloride Cl(-) channels has been implicated to play important roles in modulating cell cycle and cell migration. The aim of this study was to determine whether volume-activated Cl(-) channels are involved in cell-cycle-dependent regulation of cell migration in HeLa cells. Using techniques including cell-cycle synchronization, transwell migration assays and the patch-clamp technique, we demonstrate in this study that both the expression of volume-activated chloride current (I(Cl,vol)) and the potential of cell migration are cell-cycle-dependent; specifically, these events were high in G(0)/G(1) phase, low in S phase, and medium in G(2)/M phase. Moreover, the mean density of I(Cl,vol) was positively correlated to the rate of cell migration during cell-cycle progression. Additionally, endogenous suppression of I(Cl,vol) by transfecting cells with ClC-3 antisense oligonucleotides arrested cells in S phase and slowed cell migration. Collectively, our results suggest that volume-activated Cl(-) channels contribute to the cell-cycle-dependent regulation of cell migration.
Mao
Jianwen
J
Institute of Basic Medical Sciences and Department of Biology, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangdong, China. jianwenmao@hotmail.com
Chen
Lixin
L
Xu
Bin
B
Wang
Lijing
L
Wang
Weizhang
W
Li
Ming
M
Zheng
Min
M
Li
Hongzhi
H
Guo
Jiao
J
Li
Weidong
W
Jacob
Tim J C
TJ
Wang
Liwei
L
D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement; D018118:Chloride Channels / Q000187:drug effects / Q000502:physiology*; D003703:Demecolcine / Q000494:pharmacology; D004594:Electrophysiology / Q000379:methods; D005260:Female; D016195:G2 Phase; D006367:HeLa Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D008938:Mitosis; D018408:Patch-Clamp Techniques; D016196:S Phase; D002583:Uterine Cervical Neoplasms
D018118:Chloride Channels; D006918:Hydroxyurea; D003703:Demecolcine