Microarray analysis of gene expression in rat cortical neurons exposed to hyperbaric air and oxygen.
To gain a global view of the genomic response of neurons to normobaric and hyperbaric hyperoxic stress, we performed a microarray analysis of gene expression after exposure to varying levels of partial oxygen pressures. Rat neurons were exposed to normobaric hyperoxia, hyperbaric (2, 4, and 6 atmosphere absolute) air or hyperbaric O(2). We identified 183 genes significantly altered (increased or decreased >or=1.5-fold) in response to pressure and/or oxidative stress. Among them, 17 genes changed in response to all exposure conditions. More genes were altered in response to hyperbaric air than hyperbaric O(2). The altered genes included factors associated with stress responses, transport/neurotransmission, signal transduction, and transcription factors. The results may serve as guidance for selection of biomarkers of hyperoxia and hyperbaric O(2) response and provide a starting point for further studies to investigate the global molecular mechanisms underlying hyperbaric oxidative stress.
Chen
Ye
Y
Undersea Medicine Department, Naval Medical Research Center, Silver Spring, MD 20910, USA.
Nadi
N Suzan
NS
Chavko
Mikulas
M
Auker
Charles R
CR
McCarron
Richard M
RM
D000388:Air; D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D020869:Gene Expression Profiling; D006931:Hyperbaric Oxygenation / Q000009:adverse effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D010100:Oxygen / Q000633:toxicity*; D010313:Partial Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D010100:Oxygen