title: Skin exposure to weak and moderate contact allergens induces IFNgamma production by lymph node cells of CD4+ T-cell-depleted mice.
abstract: Allergic contact dermatitis (ACD) is mediated by hapten-specific CD8+ T cells and downregulated by CD4+ T cells. We have recently shown in a model of ACD to weak haptens that priming of IFNgamma-producing CD8+ T cells and the development of skin inflammation could be obtained in mice deficient in CD4+ T cells. Here we show that IFNgamma production by lymph node (LN) cells draining the site of skin sensitization of CD4+ T-cell-deficient mice is a marker of the sensitizing properties of weak haptens. LN cells from mice sensitized as in the classical local lymph node assay (LLNA) were recovered at day 5, then cultured for 20 hours in the presence of submitogenic doses of phytohemagglutinin, and finally tested for the production of IFNgamma. Results show that: (i) production of INFgamma by LN cells was induced by weak and moderate allergens in a dose-dependent fashion; (ii) the magnitude of IFNgamma production paralleled the sensitizing properties of allergens allowing to classify them as moderate or weak haptens; (iii) chemicals without sensitizing properties were unable to stimulate IFNgamma production by LN cells. Therefore, the IFNgamma LLNA appears as a sensitive, specific, and robust assay to detect weak contact allergens.
authors.affiliation: Faculté de Médecine, Université Lyon1, Lyon-Sud, France. marc.vocanson@inserm.fr
authors.forename: Marc
authors.initials: M
authors.lastname: Vocanson
authors.forename: Anca
authors.initials: A
authors.lastname: Hennino
authors.forename: Aurore
authors.initials: A
authors.lastname: Rozières
authors.forename: Magalie
authors.initials: M
authors.lastname: Cluzel-Tailhardat
authors.forename: Gaelle
authors.initials: G
authors.lastname: Poyet
authors.forename: Magalie
authors.initials: M
authors.lastname: Valeyrie
authors.forename: Josette
authors.initials: J
authors.lastname: Bénetière
authors.forename: Rosine
authors.initials: R
authors.lastname: Tédone
authors.forename: Dominique
authors.initials: D
authors.lastname: Kaiserlian
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Nicolas
mesh_terms: D000485:Allergens / Q000276:immunology / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000473:pathology*; D017449:Dermatitis, Allergic Contact / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D006241:Haptens / Q000276:immunology / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism*; D023441:Local Lymph Node Assay; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008212:Lymphocyte Depletion / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D010835:Phytohemagglutinins / Q000494:pharmacology; D012680:Sensitivity and Specificity; D012867:Skin / Q000187:drug effects* / Q000276:immunology*
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D006241:Haptens; D010835:Phytohemagglutinins; D007371:Interferon-gamma