Chemokine receptors expression on T cells and response to HAART among chronic HIV-1-infected subjects.
Chemokines receptors are used by HIV-1 for entry into CD4(+) T cells. The beta-chemokines are capable of inhibiting HIV replication. This study determined the CCR5 and CXCR4 expression on T cells in HIV-1-infected patients treated with HAART. The successfully treated group (plasma viral load <400 copies/mL), when compared with the failure group (plasma viral load >400 copies/mL), had higher median CD4(+) T cells count (583 and 245 cells/mm(3); respectively, p< 0.0001). The failure patients had higher numbers and intensity of CCR5 and CXCR4-expressing T cells. Successfully treated patients were able to normalize the co-receptors expression-over on T cells. The viremic group showed higher CCR5 expression on CD4(+) T cells and lower number of cells; CCR5 expression was normalized in the aviremic group; the naïve group showed lower CCR5 expression and higher numbers of CD4 T cells; all groups showed normal CXCR4 expression compared to healthy controls. These findings may have clinical implications, since down-regulation of these co-receptors could be an adjuvant strategy for anti-HIV treatment.
Brito
Adriana
A
Laboratory of Immunology and Dermatology, Department of Dermatology, Sao Paulo University Medical School, Sao Paulo, SP, Brazil.
Rigato
Paula O
PO
Almeida
Alexandre
A
Fernandes
Simone S
SS
Gonsalez
Cláudio R
CR
Fonseca
Luiz A M
LA
Duarte
Alberto J S
AJ
Casseb
Jorge
J
D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D018791:CD4 Lymphocyte Count; D005260:Female; D015658:HIV Infections / Q000188:drug therapy* / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019713:Receptors, CCR5 / Q000096:biosynthesis*; D019718:Receptors, CXCR4 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology*; D014766:Viremia
C527276:CXCR4 protein, human; D019713:Receptors, CCR5; D019718:Receptors, CXCR4