Application of polyammonium cations to enzyme-immobilized electrode: voltammetric behavior of polycation-hexacyanoferrate anion complexes and applicability as electron-transfer mediator.
Cyclic voltammograms of the aqueous solution containing polyammonium cations and the [Fe(CN)6]4-. anion at a plastic-formed carbon or platinum electrode were recorded. Polyammonium salts comprising quaternary ammonium in the main chain or sub chain and those comprising primary ammonium were tested. Under certain conditions, well-developed anodic and cathodic peak currents with the midpoint potentials different from that when the polyammonium cation is absent, that is, the midpoint potential of [Fe(CN)6]3- + e- = [Fe(CN)6]4- were exhibited, indicating that the [Fe(CN)6]4-/3- anionic species associate with the polyammonium cations tested to form the polycation-[Fe(CN)6]4-/3- complex species. The polycation complex species can be easily localized around the electrode surface with a dialysis membrane. An application of the polycation complex species as an electron-transfer mediator in the catalytic electrode with a redox enzyme was examined.
Katano
Hajime
H
Department of Bioscience, Fukui Prefectural University, Japan. hajime@fpu.ac.jp
Tatsumi
Hirosuke
H
Hibi
Takao
T
Ikeda
Tokuji
T
Tsukatani
Toshihide
T
D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized; D005295:Ferrocyanides / Q000737:chemistry*; D010084:Oxidation-Reduction; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D000644:Quaternary Ammonium Compounds
D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D011073:Polyamines; D000071228:Polyelectrolytes; D000644:Quaternary Ammonium Compounds; C020354:hexacyanoferrate II