Correlating blood immune parameters and a CCT7 genetic variant with the shedding of Salmonella enterica serovar Typhimurium in swine.
The porcine response to Salmonella infection is critical for control of Salmonella fecal shedding and the establishment of Salmonella carrier status. In this study, 40 crossbred pigs were intranasally inoculated with Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) and monitored for Salmonella fecal shedding and blood immune parameters at 2, 7, 14 and 20 days post-inoculation (dpi). Using a multivariate permutation test, a positive correlation was observed between Salmonella Typhimurium shedding levels at 2 and 7dpi and serum interferon-gamma (IFNgamma) levels at 2dpi (p<0.05), with Salmonella being shed in greater numbers from animals with higher IFNgamma levels. A positive correlation was also observed between IFNgamma levels and the number of banded neutrophils (2dpi), circulating neutrophils (7 and 14dpi), monocytes (7dpi), and white blood cells (WBCs) (7, 14 and 20dpi). We have further performed association studies on these immune response parameters as well as shedding status of the Salmonella-infected pigs with a single nucleotide polymorphism (SNP) in the porcine gene CCT7, previously shown by our group to be transcriptionally up-regulated in swine experimentally inoculated with Salmonella Typhimurium. Our analyses with the 40 pigs suggest a positive association (p=0.0012) of SNP genotype A/G at position AK240296.c1153G>A of the CCT7 gene with Salmonella shedding at 7dpi compared to the G/G homozygote genotype. Linking specific genes and genetic polymorphisms with the porcine immune response to Salmonella infection and shedding may identify potential markers for carrier pigs as well as targets for disease diagnosis, intervention and prevention.
Uthe
J J
JJ
USDA, ARS, National Animal Disease Center, Ames, IA 50010, USA; Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
Wang
Y
Y
Qu
L
L
Nettleton
D
D
Tuggle
C K
CK
Bearson
S M D
SM
D000818:Animals; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000821:virology; D005260:Female; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D012481:Salmonella Infections, Animal / Q000097:blood / Q000235:genetics / Q000276:immunology; D012485:Salmonella typhi / Q000235:genetics* / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014435:Typhoid Fever / Q000662:veterinary*; D017201:Virus Shedding / Q000276:immunology*
D017931:DNA Primers; D004279:DNA, Viral; D007371:Interferon-gamma