Nanoscale imaging of molecular positions and anisotropies.
Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.
Gould
Travis J
TJ
Department of Physics and Astronomy and Institute for Molecular Biophysics, 5709 Bennett Hall, University of Maine, Orono, Maine 04469, USA.
Gunewardene
Mudalige S
MS
Gudheti
Manasa V
MV
Verkhusha
Vladislav V
VV
Yin
Shu-Rong
SR
Gosse
Julie A
JA
Hess
Samuel T
ST
D000818:Animals; D016880:Anisotropy; D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*
D001704:Biopolymers