title: A Simple and Quick Method to Isolate Nuclear Extracts from Pupae of Drosophila melanogaster.
abstract: Preparation of nuclear extracts is a critical step for biochemical identification of factors which function in the nuclei such as transcription factors. We have established a new method to prepare nuclear extracts from prepupae or pupae of Drosophila melanogaster. The method is simple and particularly useful for small-scale isolation from materials that are hard to get in large amounts such as specific tissues or animal bodies covered by pupal cases.
authors.affiliation: Advanced Research Institute for Science and Engineering, Waseda University, Higashi-Fushimi, Nishitokyo, 202-0021, Japan.
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Susumu
authors.initials: S
authors.lastname: Hirose
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ueda