Sensitive fluorescent staining for proteomic analysis of proteins in 1-D and 2-D SDS-PAGE and its comparison with SYPRO Ruby by PMF.
A novel fluorescence-based method for protein staining on SDS polyacrylamide gel is described. In this method, proteins are stained using counterion (palmatine and SDS) staining solution, which is inexpensive, easy to perform, and does not involve a destaining step. Fixing and staining of proteins using the counterion protocol take less than an hour. As little as 2 ng of protein can be detected. Another interesting feature of the staining protocol described here is the compatibility with MALDI-TOF MS which shows a similar number of identification score and sequence coverage compared with those of SYPRO Ruby.
Cong
Wei-Tao
WT
Research Institute of Drug Development, College of Pharmacy, Chonnam National University, Kwangju, South Korea.
Hwang
Sun-Young
SY
Jin
Li-Tai
LT
Choi
Jung-Kap
JK
D001600:Berberine Alkaloids; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D006801:Humans; D009942:Organometallic Compounds; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001600:Berberine Alkaloids; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D011506:Proteins; C515601:Sypro Ruby; D012967:Sodium Dodecyl Sulfate; C005413:palmatine