Comparison of genetic and cytogenetic maps of hexaploid wheat (Triticum aestivum L.) using SSR and DArT markers.
A number of technologies are available to increase the abundance of DNA markers and contribute to developing high resolution genetic maps suitable for genetic analysis. The aim of this study was to expand the number of Diversity Array Technology (DArT) markers on the wheat array that can be mapped in the wheat genome, and to determine their chromosomal location with respect to simple sequence repeat (SSR) markers and their position on the cytogenetic map. A total of 749 and 512 individual DArT and SSR markers, respectively, were identified on at least one of four genetic maps derived from recombinant inbred line (RIL) or doubled haploid (DH) populations. A number of clustered DArT markers were observed in each genetic map, in which 20-34% of markers were redundant. Segregation distortion of DArT and SSR markers was also observed in each mapping population. Only 14% of markers on the Version 2.0 wheat array were assigned to chromosomal bins by deletion mapping using aneuploid lines. In this regard, methylation effects need to be considered when applying DArT marker in genetic mapping. However, deletion mapping of DArT markers provides a reference to align genetic and cytogenetic maps and estimate the coverage of DNA markers across the wheat genome.
Francki
Michael G
MG
Department of Agriculture and Food Western Australia, South Perth, WA 6151, Australia. mfrancki@agric.wa.gov.au
Walker
Esther
E
Crawford
Allison C
AC
Broughton
Sue
S
Ohm
Herbert W
HW
Barclay
Iain
I
Wilson
Robin E
RE
McLean
Robyn
R
D002874:Chromosome Mapping; D005819:Genetic Markers; D005838:Genotype; D011123:Polyploidy; D011995:Recombination, Genetic; D014908:Triticum / Q000235:genetics*
D005819:Genetic Markers