A real-time Taqman method for hepatitis C virus genotyping and methods for further subtyping of isolates.
The HCV genome is highly heterogeneous; more and more genotypes, each with several distinct subtypes, are being identified around the world. Knowledge of genotype is important for planning of treatment regimes, whereas subtype identification is useful in epidemiological studies and outbreak investigation. We describe HCV genotyping and subtyping assays, based on real-time PCR, that are sensitive, specific, and reliable. These assays provide fast, accurate, and convenient methods for HCV genotyping/subtyping to support clinical practice.
Rolfe
Kathryn J
KJ
Health Protection Agency Cambridge Microbiology and Public Health Laboratory Addenbrooke's Hospital, Cambridge, UK. kathryn.rolfe@addenbrookes.nhs.uk
Wreghitt
Tim G
TG
Alexander
Graeme J M
GJ
Curran
Martin D
MD
D000465:Algorithms; D001483:Base Sequence; D015342:DNA Probes / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D048348:Reverse Transcription; D012680:Sensitivity and Specificity; D019914:Taq Polymerase; D013997:Time Factors
D015342:DNA Probes; D019914:Taq Polymerase