Induction of Mac-2BP by nerve growth factor is regulated by the PI3K/Akt/NF-kappaB-dependent pathway in the HEK293 cell line.
Mac-2BP is a ligand of the galectin family that has been suggested to affect tumor proliferation and metastasis formation. We assessed Mac-2BP expression at the transcriptional and translational levels to evaluate nerve growth factor (NGF)-induced Mac-2BP expression. A time kinetic analysis using reverse transcription-polymerase chain reaction showed that NGF-induced Mac-2BP transcript levels were 4-5 times higher than in controls. Mac-2BP enzyme-linked immunosorbent assay and immunofluorescence staining showed a 2-3-fold increase in intracellular and secreted Mac-2BP as a result of NGF stimulation. This increase was regulated by Akt activation and NF-kappaB binding. p65 and p50-NF-kappaB are major transcriptional factors in the Mac-2BP promoter region, and were shown to be regulated in accordance with the Akt activation states. Collectively, these results suggest that NGF induces Mac-2BP expression via the PI3K/Akt/NF-kappaB pathway.
Park
Yuk Pheel
YP
Stem Cell Research Center, KRIBB, Daejeon, Korea.
Choi
Seung-Chul
SC
Kim
Bo-Yeon
BY
Kim
Jong-Tae
JT
Song
Eun Young
EY
Kang
Seong Ho
SH
Yoon
Do-Young
DY
Paik
Sang-Gi
SG
Kim
Kwang Dong
KD
Kim
Jong Wan
JW
Lee
Hee Gu
HG
D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism / Q000502:physiology*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D051058:Oncogene Protein v-akt / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D011485:Protein Binding; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D015533:Transcriptional Activation / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects*
D000951:Antigens, Neoplasm; D008562:Membrane Glycoproteins; D016328:NF-kappa B; C114782:TAA90K protein, human; D004247:DNA; D020932:Nerve Growth Factor; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt