Influence of cysteamine on in vitro maturation, in vitro and in vivo fertilization of equine oocytes.
Contents The effect of cysteamine on in vitro nuclear and cytoplasmic maturation of equine oocytes collected by transvaginal ultrasound guided follicular aspiration was assessed. Oocytes were matured in vitro with (cysteamine group) or without (control group) cysteamine. The nuclear stage after DNA Hoechst staining, penetration rates after two different in vitro fertilization (IVF) techniques (IVF media with ionophore and Hepes buffer with heparin) and the embryo yield following oocyte intra-oviductal transfer were used as a criterion for assessing nuclear and cytoplasmic maturation, respectively. Contrary to the data described in other domestic species, there was no effect of cysteamine on in vitro nuclear maturation, IVF or in vivo embryonic development under our conditions. Ovum pick up yields (52%) and maturation rates (control group: 47% and cysteamine group: 55%) were similar to those previously reported. From 57 oocytes transferred to the oviduct in each group, the number of embryos collected was 10 (17%) in the control group and five in the cysteamine group (9%). Those two percentages were not statistically different (p > 0.05). No effect of IVF technique was seen on the success rate (6%) in each group.
Deleuze
S
S
Faculté de Médecine Vétérinaire, Département des Sciences Cliniques-Clinique Equine, Université de Liège, Liège, Belgium. s.deleuze@ulg.ac.be
Dubois
C S
CS
Caillaud
M
M
Bruneau
B
B
Goudet
G
G
Duchamp
G
G
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003543:Cysteamine / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects / Q000502:physiology; D004622:Embryo, Mammalian / Q000502:physiology; D005187:Fallopian Tubes; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000187:drug effects / Q000662:veterinary*; D006736:Horses; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D009865:Oocytes / Q000187:drug effects* / Q000637:transplantation / Q000648:ultrastructure; D013094:Spermatozoa / Q000502:physiology; D020858:Tissue and Organ Harvesting / Q000379:methods / Q000662:veterinary
D003543:Cysteamine