[Expression of bcl-2 and bax genes in the liver cancer cell line HepG2 after apoptosis induced by essential oils from Rosmarinus officinalis].
OBJECTIVE
To investigate the expression of bcl-2 and bax genes in the liver cancer cell line HepG2 after apoptosis induced by essential oils from Rosmarinus officinalis.


METHODS
Essential oils from Rosmarinus officinalis were collected by steam distillation, then its chemical composition was determined by GC-MS. Expression of bcl-2 and bax genes were detected by immunohistochemical method.


RESULTS
The liver cancer cell line HepG2 was treated with essential oils from Rosmarinus officinalis at various concentrations for different duration. Expression of bcl-2 gene reduced and expression of bax gene increased in a dose and time dependent manner.


CONCLUSIONS
Essential oils from Rosmarinus officinalis can affect the pattern of bcl-2 and bax genes expressions, and this may increase the apoptosis of liver cancer cell line HepG2.
Wei
Feng-Xiang
FX
The Basic College of Guangdong Pharmaceutical University, Guangzhou 510006, China. haowei727499@163.com
Liu
Jun-Xing
JX
Wang
Lin
L
Li
Hong-Zhi
HZ
Luo
Jia-Bin
JB
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000473:pathology; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D027542:Rosmarinus / Q000737:chemistry*; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
D000970:Antineoplastic Agents; D009822:Oils, Volatile; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein