title: Cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme.
abstract: Genes encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 were cloned and expressed in Escherichia coli. The purified enzymes were homodimers with a 46-kDa subunit and contained 1 mol/mol-subunit of pyridoxal 5'-phosphate. The V(max) of these enzymes catalyzing the conversion of alpha-methyl-L-serine to D-alanine via tetrahydrofolate was 22.1 U/mg (AJ110403) and 15.4 U/mg (AJ110404).
authors.affiliation: AminoScience Laboratories, Ajinomoto Co., Inc, Kawasaki, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nozaki
authors.forename: Shinji
authors.initials: S
authors.lastname: Kuroda
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kenzo
authors.initials: K
authors.lastname: Yokozeki
mesh_terms: D020561:Alphaproteobacteria / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D011232:Chemical Precipitation; D003001:Cloning, Molecular; D012696:Glycine Hydroxymethyltransferase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D020579:Sinorhizobium / Q000201:enzymology* / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D012696:Glycine Hydroxymethyltransferase