Utility of the thromboplastin-plasma cell-block technique for fine-needle aspiration and serous effusions.
(I) To assess the feasibility of thromboplastin-plasma (TP) method for cell block, (II) to concentrate the minimal cellular material from effusions and needle-rinses by block preparation and improve visual details, (III) to compare conventional cytological smears with cell blocks for final assessment, and (IV) to assess utility of immunocytochemistry (ICC) for diagnostic accuracy. Seventy cell blocks were prepared by TP technique using surplus fluid from 38 serous effusions, and for 32 ultrasonography-guided fine-needle aspiration cytology (FNAC) material, rinses of syringes and needles were collected in normal saline after conventional cytological smears. Then, cell blocks were compared with conventional smears for adequacy, morphologic preservation, and ICC. Absolute concordance seen in 66 cases (94%) between the smears and cell blocks. Advantages with the blocks were cellular concentration in a limited field and better cellular preservation with architectural pattern. Quality of ICC was comparable to that of standard controls. Diagnostic discrepancy was seen in two cases where cell blocks were positive but smears were negative. Two cell blocks were nonrepresentative. Cell block serves as a useful adjunct to traditional cytological smears. TP method is simple, cost effective, and reproducible. It is easy when compared with agar-embedding technique. Ancillary techniques like ICC can be performed successfully.
Kulkarni
Manisha B
MB
Division of Cytology, Tata Memorial Hospital, Dr. E. Borges Marg, Parel, Mumbai, India. kulkarnimanisha@hotmail.com
Desai
Sangeeta B
SB
Ajit
Dulhan
D
Chinoy
R F
RF
D001202:Ascitic Fluid / Q000473:pathology*; D044963:Biopsy, Fine-Needle / Q000379:methods*; D002469:Cell Separation; D003951:Diagnostic Errors; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D010490:Pericardial Effusion / Q000473:pathology*; D010996:Pleural Effusion / Q000473:pathology*; D013048:Specimen Handling / Q000379:methods; D013925:Thromboplastin; D016610:Tissue Embedding / Q000379:methods*
D013925:Thromboplastin