title: Avoidance response of Enchytraeus albidus in relation to carbendazim ageing.
abstract: In this study, avoidance response of Enchytraeus albidus to LUFA 2.2 soil contaminated with pesticide carbendazim was investigated. The aim was to clarify minimal test duration and temporal changes in avoidance response due to contamination ageing. Firstly, the concentration causing 50% avoidance (EC(50)) was determined as 7.6 mg/kg. Then, test duration needed to reach this value (ET(50)=approximately 18 h) was identified. Finally, the capability of E. albidus avoidance test to reflect the changes of pollutant bioavailability was tested. The soil was spiked with carbendazim at the EC(50) concentration 1, 14, or 28 days before the test started and avoidance effects of fresh versus aged contamination were compared. The results indicated that enchytraeids preferred soil contaminated for 28 days prior to assay where carbendazim was probably less bioavailable than in freshly spiked soil. Our results open an interesting research area of potential use of avoidance tests for contaminant bioavailability assessment.
authors.affiliation: Research Centre for Environmental Chemistry and Ecotoxicology (RECETOX), Faculty of Science, Masaryk University, Kamenice 126/3, Brno CZ-62500, Czech Republic. kobeticova@recetox.muni.cz
authors.forename: Klára
authors.initials: K
authors.lastname: Kobeticová
authors.forename: Jakub
authors.initials: J
authors.lastname: Hofman
authors.forename: Ivan
authors.initials: I
authors.lastname: Holoubek
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002219:Carbamates / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods; D004924:Escape Reaction / Q000187:drug effects*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D009835:Oligochaeta / Q000187:drug effects* / Q000502:physiology; D012989:Soil Pollutants / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D001562:Benzimidazoles; D002219:Carbamates; D005659:Fungicides, Industrial; D012989:Soil Pollutants; C006698:carbendazim