title: A proteoliposome based formulation administered by the nasal route produces vibriocidal antibodies against El Tor Ogawa Vibrio cholerae O1 in BALB/c mice.
abstract: A vaccine candidate against the enteric pathogen Vibrio cholerae was developed based on a proteoliposome (PL) formulation using a wild type strain C7258, V. cholerae O1, El Tor Ogawa as part of strategy to develop a combined formulation against enteric diseases preventable by the stimulation of the mucosal immune system. A detergent extraction method was applied to obtain the PL. Scanning electron microscopy and molecular exclusion chromatography showed the presence of two PL populations. Photon correlation spectroscopy studies were then carried out to evaluate the size (169.27+/-3.85nm), polydispersity (0.410) and zeta potential (-23.28+/-1.21mV) of the PL. SDS-PAGE and Western blot analysis revealed the presence of lipopolysaccharide (LPS), mannose-sensitive haemagglutinin (MSHA) and a range of outer membrane proteins, including OmpU. BALB/c mice were immunized intranasally with two doses of PL containing 25mug of LPS each 28 days apart. The mice showed high anti-LPS IgG titres (3.36+/-0.235) and vibriocidal antibodies (3.70+/-0.23) after two weeks from last dose. These results show for the first time that PL can be obtained from V. cholerae O1 and when administer by intranasal route has the potential to protect against this pathogen.
authors.affiliation: Instituto Finlay, Centro de Investigación y Producción de Vacunas, Ave. 27, No. 19805, La Lisa, A. P. 16017 Cod. 11600, Ciudad de La Habana, Cuba. jlperez@finlay.edu.cu
authors.forename: José Luis
authors.initials: JL
authors.lastname: Pérez
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Acevedo
authors.forename: Adriana
authors.initials: A
authors.lastname: Callicó
authors.forename: Yovania
authors.initials: Y
authors.lastname: Fernández
authors.forename: Bárbara
authors.initials: B
authors.lastname: Cedré
authors.forename: Gemma
authors.initials: G
authors.lastname: Año
authors.forename: Lissette
authors.initials: L
authors.lastname: González
authors.forename: Gustavo
authors.initials: G
authors.lastname: Falero
authors.forename: Arturo
authors.initials: A
authors.lastname: Talavera
authors.forename: Oliver
authors.initials: O
authors.lastname: Pérez
authors.forename: Luis
authors.initials: L
authors.lastname: García
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002771:Cholera / Q000276:immunology / Q000517:prevention & control*; D022121:Cholera Vaccines / Q000008:administration & dosage / Q000276:immunology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011510:Proteolipids / Q000008:administration & dosage*; D041241:Vibrio cholerae O1 / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D022121:Cholera Vaccines; D011510:Proteolipids; C020485:proteoliposomes