title: Analysis of gene transcription alterations at the blastocyst stage related to the long-term consequences of in vitro culture in mice.
abstract: We have reported that in vitro culture (IVC) of preimplantation mouse embryos in the presence of FCS produces long-term effects (LTE) on development, growth and behaviour of the offspring at adult age. To analyse the mechanisms underlying this phenomenon, we have examined development and global alterations in gene expression in the mouse blastocysts produced in the presence of FCS, conditions known to be suboptimal and that generate LTE. Embryos cultured in vitro in KSOM and in KSOM+FCS had a reduced number of cells in the inner cell mass at the blastocyst stage compared with in vivo derived embryos; however, only culture in KSOM+FCS leads to a reduction in the number of trophoblast cells. Gene expression levels were measured by comparison among three groups of blastocysts (in vivo, IVC in KSOM and IVC in KSOM+FCS). Different patterns of gene expression and development were found between embryos cultured in vitro or in vivo. Moreover, when we compared the embryos produced in KSOM versus KSOM+FCS, we observed that the presence of FCS affected the expression of 198 genes. Metabolism, proliferation, apoptosis and morphogenetic pathways were the most common processes affected by IVC. However, the presence of FCS during IVC preferentially affected genes associated with certain molecular and biological functions related to epigenetic mechanisms. These results suggest that culture-induced alterations in transcription at the blastocyst stage related to epigenetic mechanisms provide a foundation for understanding the molecular origin at the time of preimplantation development of the long-term consequences of IVC in mammals.
authors.affiliation: Departamento de Reproducción Animal, INIA, Madrid, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Fernández-González
authors.forename: Juan
authors.initials: J
authors.lastname: de Dios Hourcade
authors.forename: Irene
authors.initials: I
authors.lastname: López-Vidriero
authors.forename: Alberto
authors.initials: A
authors.lastname: Benguría
authors.forename: Fernando Rodríguez
authors.initials: FR
authors.lastname: De Fonseca
authors.forename: Alfonso
authors.initials: A
authors.lastname: Gutiérrez-Adán
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D003470:Culture Media; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000235:genetics; D044127:Epigenesis, Genetic; D015870:Gene Expression; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008808:Mice, Inbred CBA; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic
chemical_list: D003470:Culture Media