title: Dorsal tunica vaginalis graft plus onlay preputial island flap urethroplasty: experimental study in rabbits.
abstract: OBJECTIVE
  To assess the use of tunica vaginalis graft plus onlay preputial island flap in urethral reconstructive surgery in rabbits through histopathology.
  
  
  METHODS
  We developed an experimental model of urethroplasty that resembles one-stage complex hypospadias surgery with divided urethral plate. The tunica vaginalis graft is dorsally placed to recreate the urethral plate and the internal preputial island flap is placed onlay to complete the urethroplasty. Sixteen animals were divided into four equal groups and sacrificed at 2, 4, 8 and 12 weeks after surgery, the penis being sent for histopathological evaluation.
  
  
  RESULTS
  There were no deaths related to the procedure or wound breakdown; all rabbits voided spontaneously after surgery. Two urethrocutaneous fistulae were found. Microscopically, good tissue integration was observed, the tunica vaginalis mesothelium was gradually replaced by a more stratified epithelial lining, similar to the urothelial lining of the native urethra. The stratified squamous non-keratinized lining of the internal preputial island flap also changed into a thinner epithelial lining with only 4-5 cell layers. Two urethral diverticula were found.
  
  
  CONCLUSIONS
  A tunica vaginalis graft placed dorsally plus an onlay internal preputial island flap was shown to be a successful technique for urethroplasty in an animal model.
authors.affiliation: Federal University of São Paulo, São Paulo, SP 04024-002, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Leslie
authors.forename: Luiz L
authors.initials: LL
authors.lastname: Barboza
authors.forename: Petrus O
authors.initials: PO
authors.lastname: Souza
authors.forename: Paulo S
authors.initials: PS
authors.lastname: Silva
authors.forename: Rosana
authors.initials: R
authors.lastname: Delcelo
authors.forename: Valdemar
authors.initials: V
authors.lastname: Ortiz
authors.forename: Antonio
authors.initials: A
authors.lastname: Macedo
mesh_terms: D000818:Animals; D007021:Hypospadias / Q000601:surgery*; D007249:Inflammation / Q000473:pathology; D008297:Male; D023421:Models, Animal; D010413:Penis / Q000601:surgery; D011183:Postoperative Complications / Q000276:immunology / Q000473:pathology; D011817:Rabbits; D012704:Serous Membrane / Q000473:pathology / Q000637:transplantation*; D013524:Surgical Flaps; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014521:Urethra / Q000601:surgery; D013521:Urologic Surgical Procedures, Male / Q000379:methods*