LYRM1, a novel gene promotes proliferation and inhibits apoptosis of preadipocytes.
OBJECTIVE
To characterize a novel gene, Homo sapiens LYR motif containing 1 (LYRM1), that is highly expressed in omental adipose tissue of obese subjects.


RESULTS
RT-PCR and western blot analysis confirmed that both mRNA and protein levels of LYRM1 were higher in omental adipose tissue of obese subjects than in normal weight subjects. RT-PCR analysis demonstrated that LYRM1 expression is widely distributed, with the highest levels of expression occurring in adipose tissue. A fusion protein of LYRM1 and green fluorescent protein as well as western blot analysis were used to identify the subcellular localization of LYRM1 in the nucleus. Based on Oil red O staining and the expression profile of specific differentiation markers, ectopic LYRM1 expression was not found to significantly affect adipogenesis. MTT assays and cell cycle analysis showed that LYRM1 promotes preadipocyte proliferation, and data from annexin V-FITC and caspase-3 activity assays further determined that LYRM1 can inhibit apoptosis of preadipocytes.


CONCLUSIONS
By increasing cell proliferation and lowering the rate of apoptosis, LYRM1 has the potential to modulate the size of the preadipocyte pool and influence adipose tissue homeostasis.
Qiu
Jie
J
Department of Pediatrics, Nanjing Maternity and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China.
Gao
Chun-Lin
CL
Zhang
Min
M
Chen
Rong-Hua
RH
Chi
Xia
X
Liu
Feng
F
Zhang
Chun-Mei
CM
Ji
Chen-Bo
CB
Chen
Xiao-Hui
XH
Zhao
Ya-Ping
YP
Li
Xiao-Nan
XN
Tong
Mei-Ling
ML
Ni
Yu-Hui
YH
Guo
Xi-Rong
XR
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D019556:COS Cells; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*
D000906:Antibodies; D051017:Apoptosis Regulatory Proteins; C570850:LYR motif containing 1 protein, mouse; C538836:LYRM1 protein, human; D011506:Proteins