Coating of objects introduced into the oviduct of pseudopregnant rabbit does.
This investigation addresses the possibility of providing mouse embryos or other foreign objects with a protective mucin coat by transferring them into the oviduct of a live rabbit doe. Mouse embryos at the 8 or 16-cell stage, rabbit oocytes and latex spheres resembling mouse embryos in size were transferred to the ligated oviducts of ovulation-induced rabbit does. The does were killed 24 h later to have their oviducts flushed. A large proportion of the latex spheres (89%) and of the ovulated oocytes of the recipient does (92%) was recovered. The recovery rates for transferred rabbit oocytes, either intact or with the zona pellucida removed, were 61% and 51%, respectively, whereas that for mouse embryos was extremely poor (20%). Rabbit oocytes with or without zona were enveloped in a thick mucin coat regardless whether they had been transferred or ovulated by the recipients. The same applied to empty rabbit zonae. Mouse embryos and latex spheres were also covered by a mucin coat, but it was four times thinner. While residing in the rabbit oviduct, the mouse embryos continued developing to a stage comparable to what would have been expected in situ. During the subsequent in vitro culture, mouse embryos continued developing to the expanded blastocyst stage. They did, yet, not hatch from the zona. It may be concluded that particles of various origins, when placed into the oviduct of ovulated rabbit does, will be provided with a mucin covering which is, however, considerably thinner than that surrounding oocytes or zonae pellucidae originating from rabbits.
El-Gayar
M
M
Institute of Animal Husbandry and Genetics, University of Goettingen, Albrecht-Thaer-Weg 3, Goettingen, Germany.
Holtz
W
W
D000818:Animals; D015925:Cryopreservation / Q000662:veterinary; D003451:Cryoprotective Agents; D004624:Embryo Transfer / Q000662:veterinary*; D005187:Fallopian Tubes / Q000502:physiology*; D005260:Female; D008297:Male; D051379:Mice; D008863:Microspheres; D009077:Mucins / Q000502:physiology*; D009865:Oocytes; D010060:Ovulation; D011555:Pseudopregnancy; D011817:Rabbits / Q000502:physiology*; D014021:Tissue Preservation / Q000662:veterinary
D003451:Cryoprotective Agents; D009077:Mucins