Disturbance of cellular glucose transport by two prevalently used fluoroquinolone antibiotics ciprofloxacin and levofloxacin involves glucose transporter type 1.
Dysglycemia and central nervous system (CNS) complications are the known adverse effects of fluoroquinolone antibiotics. Ciprofloxacin and levofloxacin are among the most prescribed antibiotics. In this study we demonstrate that ciprofloxacin and levofloxacin disturb glucose transport into HepG2 cells and such inhibition is associated with inhibited glucose transporter type 1 (GLUT1) function. When exposed to ciprofloxacin or levofloxacin at maximum plasma concentrations (C(max)) and 5x of C(max) concentrations, GLUT1 mRNA expression, cell surface GLUT1 protein expression and glucose uptake were significantly reduced. These findings imply that disturbed cellular glucose transport and GLUT1 function may underlie the dysglycemic and CNS effects of ciprofloxacin and levofloxacin.
Ge
Daniel Tianfang
DT
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
Law
Pui Ying Peggy
PY
Kong
Siu-Kai
SK
Ho
Yuan-Yuan
YY
D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D001692:Biological Transport; D045744:Cell Line, Tumor; D002939:Ciprofloxacin / Q000009:adverse effects*; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1 / Q000096:biosynthesis / Q000502:physiology*; D006801:Humans; D064704:Levofloxacin; D015242:Ofloxacin / Q000009:adverse effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D051272:Glucose Transporter Type 1; C495677:SLC2A1 protein, human; D002939:Ciprofloxacin; D064704:Levofloxacin; D015242:Ofloxacin; D005947:Glucose