Quasi-equilibrium assumption in enzyme kinetics. Necessary and sufficient conditions and accuracy of its application for single-substrate reactions.
Steady-state kinetics of compulsory-ordered single-substrate irreversible and reversible enzyme reactions with two, three, and arbitrary number of intermediates were observed. Necessary and sufficient conditions for application of the quasi-equilibrium assumption and restrictions of this assumption were found in cases of two and three intermediates in the equilibrium segment. For all cases, accuracy of the quasi-equilibrium assumption was evaluated.
Vrzheshch
P V
PV
Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119992, Russia. peter@genebee.msu.ru
D002384:Catalysis; D003198:Computer Simulation; D004735:Energy Transfer; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics
D004798:Enzymes