The effects of ketorolac tromethamine and baicalein on the levels of inflammatory factors in human synoviocytes.
This study examined the effects of ketorolac tromethamine (KT) and baicalein (BE) on the levels of inflammatory factors in human synoviocytes. The fibroblast-like synoviocytes (FLS) cells were used to determine the possible regulatory effects of KT and BE (KTBE) on the levels of inflammatory factors in FLS cells. In addition, the levels of TNF-alpha, IL-6, and IL-1beta mRNA expression in FLS cells induced by a TNF-alpha and IL-1beta co-treatment were largely inhibited by a KTBE treatment. The level of FLS cells proliferation was increased by IL-1beta and TNF-alpha, and strongly inhibited by KTBE treatment. The production of oxygen species (ROS) was inhibited by KTBE in FLS cells. KTBE appears to regulate the levels of mRNA that are important for regulating RA progression.
Yang
Jae-Heon
JH
Department of Pharmacy, Woosuk University, Samrye 565-701, Korea.
Yun
Mi-Young
MY
Lee
Nam-Hee
NH
Kim
Dae-Keun
DK
Kim
Young-Il
YI
Noh
Young-Hee
YH
Kim
Tae-Youl
TY
Yoon
Se-Won
SW
Shin
Sang-Chul
SC
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D020911:Ketorolac Tromethamine / Q000494:pharmacology*; D011448:Prostaglandin Antagonists / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence; D013582:Synovial Fluid / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D044950:Flavanones; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D011448:Prostaglandin Antagonists; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C006680:baicalein; D020911:Ketorolac Tromethamine