title: Retinoic acid receptor-related orphan receptor alpha regulates a subset of cone genes during mouse retinal development.
abstract: Color vision is supported by retinal cone photoreceptors that, in most mammals, express two photopigments sensitive to short (S-opsin) or middle (M-opsin) wavelengths. Expression of the Opn1sw and Opn1mw genes, encoding S-opsin and M-opsin, respectively, is under the control of nuclear receptors, including thyroid hormone receptor beta2 (TRbeta2), retinoid X receptor gamma (RXRgamma), and RORbeta, a member of the retinoic acid receptor-related orphan receptor (ROR) family. We now demonstrate that RORalpha, another member of the ROR family, regulates Opn1sw, Opn1mw, as well as Arr3 (cone arrestin) in the mouse retina. RORalpha expression is detected in cones by postnatal day 3 and maintained through adulthood. The retinas of staggerer mice, carrying a null mutation of RORalpha, show significant down-regulation of Opn1sw, Opn1mw, and Arr3. RORalpha acts in synergy with cone-rod homeobox transcription factor (Crx), to activate the Opn1sw promoter in vitro. Chromatin immunoprecipitation assays reveal that RORalpha directly binds to the Opn1sw promoter, Opn1mw locus control region, and the Arr3 promoter in vivo. Our data suggest that RORalpha plays a crucial role in cone development by directly regulating multiple cone genes.
authors.affiliation: Department of Anatomy, School of Medicine, Tokyo Women's Medical University, Tokyo, Japan. hfujieda@research.twmu.ac.jp
authors.forename: Hiroki
authors.initials: H
authors.lastname: Fujieda
authors.forename: Rod
authors.initials: R
authors.lastname: Bremner
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Mears
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D019390:Arrestins / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000172:deficiency; D047369:Chromatin Immunoprecipitation / Q000379:methods; D015536:Down-Regulation / Q000502:physiology*; D004622:Embryo, Mammalian; D005136:Eye Proteins; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D018398:Homeodomain Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008818:Mice, Neurologic Mutants; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism*; D017299:Rod Opsins / Q000378:metabolism; D015534:Trans-Activators / Q000502:physiology*; D014162:Transfection
chemical_list: D019390:Arrestins; D050976:Basic-Leucine Zipper Transcription Factors; D005136:Eye Proteins; D018398:Homeodomain Proteins; C084884:Nrl protein, mouse; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D018160:Receptors, Cytoplasmic and Nuclear; D017299:Rod Opsins; D015534:Trans-Activators; C109288:cone rod homeobox protein; C095854:middle-wavelength opsin; C095853:short-wavelength opsin