Efficient production of type 2 porcine circovirus-like particles by a recombinant baculovirus.
The capsid protein of PCV2 was expressed by using a recombinant baculovirus with insect Tn5 cells. A large amount of 28-kDa protein was released into the culture medium and self-assembled into PCV2-like particles (PCV2-LPs) with a buoyant density of 1.365 g/cm(3) and a diameter of 20 nm. PCV2-LPs were efficiently expressed, yielding 1 mg of purified particles per 10(7) Tn5 cells. The PCV2-LPs have antigenicity similar to that of authentic PCV2 particles, allowing us to develop a method for sensitively detecting PCV2-specific IgG antibodies. In addition, the PCV2-LPs appeared to be the most promising PCV2 vaccine candidate, by virtue of their potent immunogenicity.
Liu
Lan-Jun
LJ
Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo, 208-0011, Japan.
Suzuki
Takako
T
Tsunemitsu
Hiroshi
H
Kataoka
Michiyo
M
Ngata
Noriyo
N
Takeda
Naokazu
N
Wakita
Takaji
T
Miyamura
Tatsuo
T
Li
Tian-Cheng
TC
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D036022:Capsid Proteins / Q000096:biosynthesis* / Q000276:immunology; D002460:Cell Line; D017925:Circovirus / Q000276:immunology* / Q000648:ultrastructure; D005822:Genetic Vectors; D046529:Microscopy, Electron, Transmission; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000175:diagnosis / Q000517:prevention & control; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000276:immunology; D013552:Swine; D014765:Viral Vaccines / Q000096:biosynthesis* / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; D014765:Viral Vaccines