title: Endometrial stromal cells from women with endometriosis reveal peculiar migratory behavior in response to ovarian steroids.
abstract: OBJECTIVE
  To evaluate differences in endometrial stromal cell (ESC) migration between patients with and without endometriosis.
  
  
  METHODS
  Differences in ESC migration, cellular morphology, and cytoskeletal-actin dynamics were evaluated in response to platelet-derived growth factor-BB (PDGF-BB) and steroid hormones (17beta-estradiol and progesterone).
  
  
  METHODS
  Medical school research laboratory.
  
  
  METHODS
  Endometrial biopsy samples obtained from 43 women: 23 as controls (endometriosis excluded by laparoscopy), 20 with severe or moderate endometriosis (diagnosed by laparoscopy).
  
  
  METHODS
  ESCs were treated with and without PDGF-BB, 17beta-estradiol, and progesterone.
  
  
  METHODS
  Cellular migration was evaluated by means of chemotaxis experiments in a Boyden chamber. Cellular morphology and cytoskeletal-actin dynamics were evaluated by immunofluorescence.
  
  
  RESULTS
  Progesterone stimulated the migratory behavior of ESCs derived from women with endometriosis, while 17beta-estradiol could stimulate motility of ESCs derived from both controls and women with endometriosis, with a greater effect observed in the latter group. No difference in ESC migratory behavior after PDGF-BB treatment was observed between women with and without the disease. Also, PDGF-BB and steroid hormones could modify the organization of actin cytoskeletal structures.
  
  
  CONCLUSIONS
  Ovarian steroids differently affect the migration of ESCs derived from women with and without endometriosis. This effect is likely to involve cytoskeletal reorganization. Nongenomic signaling pathways induced by steroid hormones might have a role in this phenomenon.
authors.affiliation: Molecular Biology Laboratory, Istituto Auxologico Italiano, Milan, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Gentilini
authors.forename: Paola
authors.initials: P
authors.lastname: Vigano
authors.forename: Edgardo
authors.initials: E
authors.lastname: Somigliana
authors.forename: Lucia Maria
authors.initials: LM
authors.lastname: Vicentini
authors.forename: Michele
authors.initials: M
authors.lastname: Vignali
authors.forename: Mauro
authors.initials: M
authors.lastname: Busacca
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Di Blasio
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology; D000077214:Becaplermin; D001706:Biopsy; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D004715:Endometriosis / Q000473:pathology* / Q000503:physiopathology; D004717:Endometrium / Q000166:cytology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D043925:Angiogenesis Inducing Agents; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D011374:Progesterone; D004958:Estradiol