title: Silk-fiber immobilized lipase-catalyzed hydrolysis of emulsified sunflower oil.
abstract: Lipase was immobilized in silk fibers through glutaraldehyde cross-linking to a maximum loading of 59 U/g silk-fiber and the immobilized lipase was utilized for the hydrolysis of sunflower oil (Helianthus annuus). The hydrolytic activity of the lipase, which was poor in biphasic oil in water system, was increased significantly when the sunflower oil was emulsified in aqueous medium. The hydrolytic activities of the immobilized lipase were 48.73 +/- 1.26 U, 36.11 +/- 0.96 U, and nil when the substrate sunflower oil was used as emulsion created by a rhamnolipid biosurfactant, Triton X100, and ultrasonication, respectively. Although the efficiency of the immobilized lipase was less than 12% than the corresponding free lipase, the immobilized lipase could be reused for the biosurfactant-mediated hydrolysis of sunflower oil up to third cycle of the reaction. The yield of the fatty acids in the second, third, and fourth cycles were 49.45%, 22.91%, and 5.09%, respectively, of the yield obtained in the first cycle.
authors.affiliation: Centre for Energy, Indian Institute of Technology Guwahati, Guwahati, 781039, India.
authors.forename: Sushovan
authors.initials: S
authors.lastname: Chatterjee
authors.forename: Lepakshi
authors.initials: L
authors.lastname: Barbora
authors.forename: Swaranjit Singh
authors.initials: SS
authors.lastname: Cameotra
authors.forename: Pinakeswar
authors.initials: P
authors.lastname: Mahanta
authors.forename: Pranab
authors.initials: P
authors.lastname: Goswami
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*; D047011:Silk / Q000737:chemistry*; D000074242:Sunflower Oil
chemical_list: D004800:Enzymes, Immobilized; D010938:Plant Oils; D047011:Silk; D000074242:Sunflower Oil; D008049:Lipase