title: Root coverage with cultured gingival dermal substitute composed of gingival fibroblasts and matrix: a case series.
abstract: Cultured gingival dermal substitute (CGDS), composed of gingival fibroblasts and matrix and fabricated using tissue-engineering techniques, has been used for root coverage procedures. Fourteen sites from four patients with > or = 2 mm of Miller Class I or II facial gingival tissue recession were treated. The autologous CGDS sheet, prepared prior to surgical treatment, was grafted over the teeth with gingival recession and then covered with a coronally positioned flap. Vertical and horizontal recession was measured at baseline (prior to the surgical procedure) and 13 to 40 weeks (average: 30.7 +/- 9.6 weeks) after surgery. The average vertical and horizontal root coverage after surgery was 79.1% +/- 25.7% and 75.2% +/- 31.4%, respectively. Moreover, there was a significant increase of keratinized and attached gingival tissue at the final clinical evaluation compared with preoperative measurements (P < .05). These results demonstrate CGDS as a promising grafting material for use with root coverage procedures in periodontal therapy.
authors.affiliation: Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Murata
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Okuda
authors.forename: Manabu
authors.initials: M
authors.lastname: Momose
authors.forename: Kentarou
authors.initials: K
authors.lastname: Kubo
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Kuroyanagi
authors.forename: Larry F
authors.initials: LF
authors.lastname: Wolff
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D005260:Female; D005347:Fibroblasts / Q000637:transplantation*; D005782:Gels; D005881:Gingiva / Q000166:cytology* / Q000473:pathology; D005889:Gingival Recession / Q000473:pathology / Q000601:surgery*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D007633:Keratins; D008297:Male; D008875:Middle Aged; D013524:Surgical Flaps; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014092:Tooth Root / Q000473:pathology / Q000601:surgery*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D005782:Gels; C059821:atelocollagen; D007633:Keratins; D006820:Hyaluronic Acid; D003094:Collagen