title: Increased platelet adhesion under flow conditions is induced by both thalassemic platelets and red blood cells.
abstract: Thromboembolic complications are not uncommon in thalassemia. Previous studies suggest increased platelet aggregation and a potential role of pathological changes in the red blood cell (RBC) lipid membrane, induced by oxidative stress. In the present study, platelet adhesion and the effect of thalassemic RBC on platelet adhesion under flow conditions were evaluated, using the Cone and Plate (let) Analyzer(CPA). Twenty-two beta-thalassemia patients and 22 blood type-matched healthy controls were studied. An increased platelet adhesion (% surface coverage, SC), was observed in patients as compared to controls (p < 0.05). When platelet count and haematocrit were normalized by autologous reconstitution, a significant increase in platelet aggregation (average size, AS) was observed (p < 0.05). Increased platelet adhesion (SC and AS), was demonstrated in six patients with a history of thrombosis as compared to 16 patients without any history of thrombosis (p < or = 0.007) and in 17 splenectomized patients as compared to five non-splenectomized patients (p = 0.003). In reconstitution studies, thalassemic RBC mixed with normal platelet-rich plasma significantly increased platelet adhesion compared to normal RBC (SC p < 0.03, AS p < 0.02). Thalassemic platelets reconstituted with normal RBC, had increased aggregation (AS, p < 0.004) in comparison with normal platelets. The results indicate that increased platelet adhesion in beta-thalassemia is induced by both platelets and RBC. Increased platelet adhesion correlated with clinical thrombotic events and thus may suggest a mechanism of thrombosis in thalassemic patients. The potential application of the CPA in identifying thalassemic patients with high risk for thrombosis should be studied prospectively in a larger cohort of patients.
authors.affiliation: Coagulation Unit, Department of Haematology, Hadassah Medical Center, PO Box 12000, Jerusalem, Israel, 91120.
authors.forename: Neta
authors.initials: N
authors.lastname: Goldschmidt
authors.forename: Galia
authors.initials: G
authors.lastname: Spectre
authors.forename: Alexander
authors.initials: A
authors.lastname: Brill
authors.forename: Orly
authors.initials: O
authors.lastname: Zelig
authors.forename: Ada
authors.initials: A
authors.lastname: Goldfarb
authors.forename: Eliezer
authors.initials: E
authors.lastname: Rachmilewitz
authors.forename: David
authors.initials: D
authors.lastname: Varon
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D016022:Case-Control Studies; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D010979:Platelet Function Tests; D011673:Pulsatile Flow; D013156:Splenectomy; D013314:Stress, Mechanical; D013923:Thromboembolism / Q000097:blood / Q000209:etiology*; D055815:Young Adult; D017086:beta-Thalassemia / Q000097:blood* / Q000150:complications / Q000601:surgery