title: Metabolism of amyloid precursor protein in COS cells transfected with a beta-secretase candidate.
abstract: Thimet oligopeptidase (TOP) is a thiol- andmetallo-dependent peptidase and has been shown to beone of the beta-secretase candidates. TOPexpressed in COS cells cleaved amyloid precursorprotein (APP) at the beta-secretase site, and wefound a proteolytic product of APP called secretedform of APP by beta-secretase (sAPPbeta) in theconditioned media. Here we demonstrate thatsAPPbeta was increased in conditioned media whenTOP was coexpressed in COS cells with APP and treatedwith an ADAM inhibitor SI-27. In addition, althoughTOP expressed in COS cell was localized at nuclei orGolgi apparatus, it exclusively colocalized at Golgiapparatus when APP was coexpressed with TOP.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo, 153-8902, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Koike
authors.forename: Z
authors.initials: Z
authors.lastname: Kouchi
authors.forename: T
authors.initials: T
authors.lastname: Kinouchi
authors.forename: T
authors.initials: T
authors.lastname: Maeda
authors.forename: H
authors.initials: H
authors.lastname: Sorimachi
authors.forename: T C
authors.initials: TC
authors.lastname: Saido
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: A
authors.initials: A
authors.lastname: Okuyama
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Ishiura