Role of 5'-UTR hairpins of the Turnip yellow mosaic virus RNA in replication and systemic movement.
Turnip yellow mosaic virus (TYMV) RNA has two hairpins in its 5' untranslated region (5'-UTR). To investigate the role of the hairpins in replication of TYMV, mutants lacking one or both of the two hairpins were constructed. The TYMV constructs were introduced into Chinese cabbage by an Agrobacterium-mediated T-DNA transfer method, called agroinfiltration. Analysis of total RNA from agroinfiltrated leaves showed that replication of the mutant TYMV RNA lacking both hairpins was about 1/100 of wild type. This mutant was also impaired in systemic spread. Deletion analysis of each hairpin revealed that both hairpins were needed for maximal replication. The deletion analysis along with sequence modification of the hairpin structure indicates that the second hairpin plays a role in efficient long-distance systemic movement of TYMV.
Shin
Hyun-Il
HI
Division of Life Sciences, Chungbuk National University, Cheongju, Korea.
Cho
Nam Jeong
NJ
Cho
Tae-Ju
TJ
D020121:5' Untranslated Regions / Q000502:physiology*; D016960:Agrobacterium tumefaciens / Q000821:virology; D001483:Base Sequence; D055029:Inverted Repeat Sequences / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017806:Tymovirus / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication / Q000235:genetics*
D020121:5' Untranslated Regions; D012367:RNA, Viral