Chemical characteristic and anticoagulant activity of the sulfated polysaccharide isolated from Monostroma latissimum (Chlorophyta).
A polysaccharide was isolated from marine green algae Monostroma latissimum, and its chemical characteristic and anticoagulant activity were investigated. The results demonstrated that the polysaccharide was high rhamnose-containing sulfated polysaccharide, and was mainly composed of 1,2-linked l-rhamnose residues with sulfate groups substituted at positions C-3 and/or C-4. The sulfated polysaccharide exhibited high anticoagulant activities by assays of the activated partial thromboplastin time (APTT) and thrombin time (TT). The anticoagulant property of the sulfated polysaccharide was mainly attributed to powerful potentiation thrombin by heparin cofactor II.
Mao
Wenjun
W
Institute of Marine Drug and Food, Ocean University of China, 5 Yusan road, Qingdao 266003, China. wenjunmqd@hotmail.com
Li
Hongyan
H
Li
Yi
Y
Zhang
Huijuan
H
Qi
Xiaohui
X
Sun
Haihong
H
Chen
Yin
Y
Guo
Shoudong
S
D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification; D000460:Chlorophyta / Q000737:chemistry*; D015844:Heparin Cofactor II; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012210:Rhamnose / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013431:Sulfates / Q000737:chemistry; D013918:Thrombin Time
D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates; D015844:Heparin Cofactor II; D012210:Rhamnose