Association between von Willebrand factor gene polymorphism and preeclampsia.
OBJECTIVE
The aims of this study were to assess whether the Msp I polymorphism of von Willebrand factor (vWF)(intron 19) gene differs between patients with preeclampsia and normal pregnancies in Chinese Han population, and to study the relationship between the Msp I polymorphism in the vWF(intron 19) gene and severity of preeclampsia symptoms within the preeclampsia group.


METHODS
In this case-control study, peripheral blood samples of 70 patients with preeclampsia and 82 normal pregnancies were genotyped for the Msp I polymorphism in vWF(intron 19) gene on the basis of a hospital-based study. The genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing techniques.


RESULTS
The genotype frequencies for M+/M+, M+/M- and M-/M- were 22.9, 45.7 and 31.4% in patients with preeclampsia, and 1.2, 36.6 and 62.2% in normal pregnancies, respectively. The frequency of the M+ allele in patients with preeclampsia was significantly higher than in normal pregnancies (45.7 vs. 19.5%).


CONCLUSIONS
The Msp I polymorphism in intron 19 (M+ allele) of vWF gene is associated with the development and the severity of preeclampsia, and may be a susceptibility factor for preeclampsia.
Sun
Chengjuan
C
Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, People's Republic of China.
Chen
Ying
Y
Zhang
Weiyuan
W
Yu
Song
S
D000328:Adult; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D014841:von Willebrand Factor / Q000235:genetics*
D014841:von Willebrand Factor