title: Cytokine and immune cell levels in peritoneal fluid and peripheral blood of women with early- and late-staged endometriosis.
abstract: OBJECTIVE
  To investigate the level of cytokines and immune cells in the peripheral blood (PB) and peritoneal fluid (PF) of different stages of endometriosis.
  
  
  METHODS
  A prospective study was conducted to include 97 women with (n 60) and without (n 37) histopathologically confirmed endometriosis. Based on rASRM classification, stage I/II and stage III/IV were categorized as early-and late-staged endometriosis. Prior to surgery, 10 ml of blood was withdrawn from antecubital vein and serum was obtained. Aliquots were made and stored at -70 degrees C until assayed for cytokines. PF was aspirated from the pouch of Douglas. Peripheral and PF samples were analyzed by ELISA in terms of IL-2, IL-4, IL-10 and IFN-gamma. Determinations of T helper, T suppressor, NK, and B cells were assessed by using cluster determinant-3 (CD-3), CD4, CD8, CD25, CD28, CD45, CD16, CD23 and antibodies against early T cell activation antigens such as CD45RA/CD45RO, CD-69 and late activation antigens such as HLA-DR. A multiparameter flow cytometry was applied to detect the cell activation antigen expression.
  
  
  RESULTS
  In terms of cytokine levels in PB and PF's of control group and early- and late-staged endometriosis cases, no significant difference was depicted in the cytokine levels (p > 0.05). Levels of immune cells did not differ between three groups (p > 0.05).
  
  
  CONCLUSIONS
  The result of this study did not show any significant difference in PB and PF cytokine and lymphocyte subgroups between normal and early- and late-staged endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Eskisehir Osmangazi University School of Medicine, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Hassa
authors.forename: H Mete
authors.initials: HM
authors.lastname: Tanir
authors.forename: B
authors.initials: B
authors.lastname: Tekin
authors.forename: S D
authors.initials: SD
authors.lastname: Kirilmaz
authors.forename: F
authors.initials: F
authors.lastname: Sahin Mutlu
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000166:cytology* / Q000378:metabolism; D016022:Case-Control Studies; D004715:Endometriosis / Q000097:blood / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D007378:Interleukins / Q000097:blood*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000166:cytology*; D010537:Peritoneum / Q000473:pathology; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D007378:Interleukins; D007371:Interferon-gamma