title: Evaluation of two gene-silencing constructs for resistance to tomato yellow leaf curl viruses in Nicotiana benthamiana plants.
abstract: Infiltration of Agrobacterium tumefaciens into intact plant leaves of N. benthamiana was used to test the efficiency of two virus-based silencing constructs conferring resistance to the closely related begomoviruses. The constructs contained the most conserved sequences of the coat protein (CP) gene and replication-associated protein (Rep) gene of Tomato yellow leaf curl Sardinia virus (Sicily strain) (TYLCSV-[Sic]). Both constructs formed a hairpin structure that enhanced the post-transcriptional gene-silencing mechanism. When agro-infiltrated plants were challenged separately with infectious viruses TYLCSV-[Sic] and Tomato yellow leaf curl virus (TYLCV), the plants showed resistance to TYLCSV-[Sic], but not to the related TYLCV.
authors.affiliation: Laboratory of Molecular Genetics, Immunology, and Biotechnology, Faculty of Science of Tunis, Tunis, Tunisia. gc_sonia@yahoo.fr
authors.forename: S
authors.initials: S
authors.lastname: Gharsallah Chouchane
authors.forename: F
authors.initials: F
authors.lastname: Gorsane
authors.forename: M K
authors.initials: MK
authors.lastname: Nakhla
authors.forename: M
authors.initials: M
authors.lastname: Salus
authors.forename: C T
authors.initials: CT
authors.lastname: Martin
authors.forename: D P
authors.initials: DP
authors.lastname: Maxwell
authors.forename: M
authors.initials: M
authors.lastname: Marrakchi
authors.forename: H
authors.initials: H
authors.lastname: Fakhfakh
mesh_terms: D000383:Agriculture / Q000379:methods*; D016960:Agrobacterium tumefaciens / Q000235:genetics; D053839:Begomovirus / Q000235:genetics / Q000472:pathogenicity*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000821:virology; D034622:RNA Interference; D014026:Nicotiana / Q000235:genetics / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D014764:Viral Proteins