Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers.
The asymmetric distribution of charged molecules between the leaflets of solid-substrate-supported phospholipid bilayers is studied using imaging ellipsometry, fluorescence microscopy, and numerical solutions of the Poisson-Boltzmann equation. Experiments are facilitated by the use of patterned substrates that allow for side-by-side comparison of lipid monolayers and supported bilayers. On silica surfaces, negatively charged lipid components are shown to be enriched in the outer leaflet of a supported bilayer system at modest salt concentrations. The approaches developed provide a general means for determining asymmetries of charged components in supported lipid bilayers.
Shreve
Andrew P
AP
Center for Integrated Nanotechnologies, Materials Physics and Applications Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Howland
Michael C
MC
Sapuri-Butti
Annapoorna R
AR
Allen
Toby W
TW
Parikh
Atul N
AN
D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D011510:Proteolipids / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
D008051:Lipid Bilayers; D008567:Membranes, Artificial; D011510:Proteolipids; D012822:Silicon Dioxide