title: Assessing the predictive value of hyaluronan binding ability for the freezability potential of human sperm.
abstract: OBJECTIVE
  To evaluate the predictive value of a sperm maturation test using the hyaluronan-binding assay (HBA) for freezability potential; and to determine the effect of freezing-thawing on HBA results.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Andrology laboratory at a teaching hospital.
  
  
  METHODS
  Candidates for sperm bank donation (n = 113) and active sperm bank donors (n = 16).
  
  
  METHODS
  Semen analyses including HBA and sperm freezing-thawing.
  
  
  METHODS
  Percentage of sperm HBA results and other sperm parameters in relation to freezing-thawing results.
  
  
  RESULTS
  The predictive value of HBA for high freezability value (>or=40% postthaw motility) was significant. However, 1- and 4-hour percentage of motility had a higher predictive value for good freezability. A better prognostic value than that of HBA resutlts was also found for sperm concentration and percentage of normal morphology. Freezing-thawing had no significant influence on HBA results.
  
  
  CONCLUSIONS
  To the best of our knowledge this is the first demonstration that sperm maturation, determined by the HBA test, has a low value for predicting freezing-thawing sperm survival.
authors.affiliation: The Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel. layogev@zahav.net.il
authors.forename: Leah
authors.initials: L
authors.lastname: Yogev
authors.forename: Sandra E
authors.initials: SE
authors.lastname: Kleiman
authors.forename: Ron
authors.initials: R
authors.lastname: Hauser
authors.forename: Amnon
authors.initials: A
authors.lastname: Botchan
authors.forename: Ofer
authors.initials: O
authors.lastname: Lehavi
authors.forename: Gedalia
authors.initials: G
authors.lastname: Paz
authors.forename: Haim
authors.initials: H
authors.lastname: Yavetz
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D015925:Cryopreservation; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D016015:Logistic Models; D008297:Male; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012372:ROC Curve; D027724:Reproductive Techniques, Assisted; D012662:Semen Preservation; D013074:Sperm Banks; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D015415:Biomarkers; D006820:Hyaluronic Acid