Isolation and characterization of alginate-degrading bacteria for disposal of seaweed wastes.
OBJECTIVE
Isolation of novel alginate degrading bacteria for the disposal of seaweed waste in composting process.


RESULTS
Decomposition of alginate polymers was checked by the 3,5-dinitrosalicylic acid (DNS) method for reducing sugar, and absorbance at 235 nm for unsaturated sugar. A bacterium A7 was isolated from wakame compost and confirmed to belong to the genus Gracilibacillus by partial 16S rDNA analysis. The optimum condition for the growth of A7 in a medium containing 5 g l(-1) of sodium alginate is as follows: pH, 8.5-9.5; NaCl, 0.5 mol l(-1); temperature, 30 degrees C and polypeptone as nutrient content, 2-5 g l(-1). In a laboratory-scale composting experiment, the alginate content in wakame compost decreased to 14.3% after 72 h of composting from an initial value of 36%, indicating the effectiveness of alginate decomposition of A7 in wakame composting.


CONCLUSIONS
The bacterium A7 was found to be alginate lyase-producing in genus Gracilibacillus and effective in degrading alginate to oligosaccharides in wakame during composting process.


CONCLUSIONS
Development of new methods for the disposal of marine wastes and production of functional products.
Tang
J-C
JC
Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education /College of Environmental Science and Engineering, Nankai University, Tianjin, China. jc_tang@163.com
Taniguchi
H
H
Chu
H
H
Zhou
Q
Q
Nagata
S
S
D000464:Alginates / Q000378:metabolism*; D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D017744:Medical Waste Disposal / Q000379:methods; D008969:Molecular Sequence Data; D010802:Phylogeny; D011133:Polysaccharide-Lyases / Q000096:biosynthesis*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012624:Seaweed / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
D000464:Alginates; D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006603:Hexuronic Acids; D007220:Industrial Waste; D017744:Medical Waste Disposal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase