Purification, crystallization and data collection of Pectobacterium chrysanthemi AcsD, a type A siderophore synthetase.
AcsD, a type A siderophore synthetase with a molecular weight of 71 140 Da from Pectobacterium chrysanthemi, has been expressed, purified and crystallized at 293 K. The protein crystallized in the primitive orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 80.3, b = 95.7, c = 161.1 A, alpha = beta = gamma = 90 degrees . Systematic absences were consistent with space group P2(1)2(1)2(1). A complete data set has been collected to 2.25 A resolution on BM14 at the ESRF. Consideration of the likely solvent content suggested that the asymmetric unit contained two molecules. Gel-filtration experiments indicated that the protein was a dimer, although self-rotation analyses did not detect a convincing twofold symmetry axis in the asymmetric unit. The protein has no convincing sequence match to any known structure and thus solution is likely to require experimental phasing.
McMahon
Stephen A
SA
Scottish Structural Proteomics Facility and Centre for Biomolecular Sciences, The University, St Andrews, Fife KY16 9ST, Scotland.
Oke
Muse
M
Liu
Huanting
H
Johnson
Kenneth A
KA
Carter
Lester
L
Kadi
Nadia
N
White
Malcolm F
MF
Challis
Gregory L
GL
Naismith
James H
JH
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D016974:Dickeya chrysanthemi / Q000201:enzymology*; D008025:Ligases / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017262:Siderophores / Q000096:biosynthesis*; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D017262:Siderophores; D008025:Ligases