title: A novel amplification target, ARHGAP5, promotes cell spreading and migration by negatively regulating RhoA in Huh-7 hepatocellular carcinoma cells.
abstract: RhoA, a member of the Rho family of small GTPases, directs the organization of the actin cytoskeleton and is involved in regulating cell shape and movement. Its activity is negatively regulated by p190-B RhoGAP (GTPase-activating protein). We investigated DNA copy number aberrations in human hepatocellular carcinoma and esophageal squamous cell carcinoma cell lines using a high-density oligonucleotide microarray and found a novel amplification at chromosomal region 14q12. We identified ARHGAP5 (the gene encoding p190-B RhoGAP) as a probable target for the amplification at 14q12, and our results showed that p190-B RhoGAP promotes cells spreading and migration by negatively regulating RhoA activity in Huh-7 hepatocellular carcinoma cells.
authors.affiliation: Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Gen
authors.forename: Kohichiroh
authors.initials: K
authors.lastname: Yasui
authors.forename: Keika
authors.initials: K
authors.lastname: Zen
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nakajima
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tsuji
authors.forename: Mio
authors.initials: M
authors.lastname: Endo
authors.forename: Hironori
authors.initials: H
authors.lastname: Mitsuyoshi
authors.forename: Masahito
authors.initials: M
authors.lastname: Minami
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Itoh
authors.forename: Shinji
authors.initials: S
authors.lastname: Tanaka
authors.forename: Masafumi
authors.initials: M
authors.lastname: Taniwaki
authors.forename: Shigeki
authors.initials: S
authors.lastname: Arii
authors.forename: Takeshi
authors.initials: T
authors.lastname: Okanoue
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D002883:Chromosomes, Human, Pair 14 / Q000648:ultrastructure; D003599:Cytoskeleton / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D018450:Disease Progression; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008113:Liver Neoplasms / Q000378:metabolism*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: C513043:ARHGAP5 protein, human; D020690:GTPase-Activating Proteins; C066737:RHOA protein, human; D004247:DNA; D020742:rhoA GTP-Binding Protein