title: The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase.
abstract: The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 353 K and utilizes an unusual promiscuous nonphosphorylative Entner-Doudoroff pathway to metabolize both glucose and galactose. It has been proposed that a part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus, in which the 2-keto-3-deoxygluconate kinase (KDGK) is promiscuous for both glucose and galactose metabolism. Recombinant S. solfataricus KDGK protein was expressed in Escherichia coli, purified and crystallized in 0.1 M sodium acetate pH 4.1 and 1.4 M NaCl. The crystal structure of apo S. solfataricus KDGK was solved by molecular replacement to a resolution of 2.0 A and a ternary complex with 2-keto-3-deoxygluconate (KDGlu) and an ATP analogue was resolved at 2.1 A. The complex suggests that the structural basis for the enzyme's ability to phosphorylate KDGlu and 2-keto-3-deoxygalactonate (KDGal) is derived from a subtle repositioning of residues that are conserved in homologous nonpromiscuous kinases.
authors.affiliation: Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, Scotland.
authors.forename: Jane A
authors.initials: JA
authors.lastname: Potter
authors.forename: Melina
authors.initials: M
authors.lastname: Kerou
authors.forename: Henry J
authors.initials: HJ
authors.lastname: Lamble
authors.forename: Steven D
authors.initials: SD
authors.lastname: Bull
authors.forename: David W
authors.initials: DW
authors.lastname: Hough
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Danson
authors.forename: Garry L
authors.initials: GL
authors.lastname: Taylor
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D004789:Enzyme Activation; D005690:Galactose / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D048229:Sulfolobus solfataricus / Q000201:enzymology*; D050801:Ternary Complex Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005942:Gluconates; D050801:Ternary Complex Factors; C002957:2-keto-3-deoxygluconate; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C021471:2-keto-3-deoxygluconokinase; D005947:Glucose; D005690:Galactose