Effect of potato suberin on Streptomyces scabies proteome.
Two-dimensional (2D) PAGE was used to detect proteins induced in Streptomyces scabies by potato suberin, a lipidic plant polymer. Nineteen up-regulated proteins were excised from 2D gels and analysed by N-terminal sequencing or tandem mass spectrometry (MS/MS). Four of the up-regulated proteins could be linked to the bacterial response to stress (AldH, GroES, TerD and LexA). Specific metabolic pathways seemed to be activated in the presence of suberin, as shown by the increased expression of specific transporters and of enzymes related not only to glycolysis, but also to nucleotide and amino acid metabolism. Suberin also appeared to influence secondary metabolism as it also caused the overproduction of the BldK proteins that are known to be involved in differentiation and secondary metabolism.
Lauzier
Annie
A
Centre SEVE, Département de biologie, Université de Sherbrooke, Sherbrooke (Qc), Canada J1K2R1.
Simao-Beaunoir
Anne-Marie
AM
Bourassa
Sylvie
S
Poirier
Guy G
GG
Talbot
Brian
B
Beaulieu
Carole
C
D001426:Bacterial Proteins / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006019:Glycolysis / Q000502:physiology; D008055:Lipids / Q000494:pharmacology*; D020543:Proteome / Q000187:drug effects*; D011198:Solanum tuberosum / Q000378:metabolism*; D013302:Streptomyces / Q000187:drug effects* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D001426:Bacterial Proteins; D008055:Lipids; D020543:Proteome; C065875:suberin