title: Sublethal infection with Salmonella Enteritidis by the natural route induces intestinal and joint inflammation in mice.
abstract: Reactive arthritis (ReA) is a sterile inflammation triggered by a distal mucosal infection, which suggests a contribution from bacterial products. Investigation on the pathogenesis of ReA is difficult because of the limited studies that can be performed in humans; therefore the availability of animal models is crucial. We hereby describe a murine model for studying the early stages of Salmonella-induced ReA. BALB/c mice infected by the natural route with a sublethal dose of S. Enteritidis showed long lasting gut inflammation, synovitis in the knee joint and a significant increase of CD4+ lymphocytes in the draining popliteal lymph nodes. S. Enteritidis infection induced histological changes in intact knees and exacerbated inflammation in previously damaged joints. Experiments performed with S. Enteritidis DeltainvG mutant suggest that the proinflammatory signalling mediated by Salmonella TTSS-1 in the gut is required for the induction of joint sequelae. Since this model is highly reproducible and easy to perform, it provides great potential for investigating both host and bacterial contributions to the early stages of ReA.
authors.affiliation: Centro de Estudios Farmacológicos y Botánicos-Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina.
authors.forename: Mariángeles
authors.initials: M
authors.lastname: Noto Llana
authors.forename: Sebastián H
authors.initials: SH
authors.lastname: Sarnacki
authors.forename: Mónica N
authors.initials: MN
authors.lastname: Giacomodonato
authors.forename: Roberto L
authors.initials: RL
authors.lastname: Caccuri
authors.forename: Guillermo A
authors.initials: GA
authors.lastname: Blanco
authors.forename: M Cristina
authors.initials: MC
authors.lastname: Cerquetti
mesh_terms: D000818:Animals; D016918:Arthritis, Reactive / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007422:Intestines / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology; D007719:Knee Joint / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D000091402:Prohibitins; D012480:Salmonella Infections / Q000382:microbiology / Q000503:physiopathology; D012477:Salmonella enteritidis / Q000472:pathogenicity*; D013585:Synovitis / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology; D014774:Virulence