Screening of potentially hormonally active chemicals using bioluminescent yeast bioreporters.
Saccharomyces cerevisiae bioluminescent bioreporter assays were developed previously to assess a chemical's estrogenic or androgenic disrupting potential. S. cerevisiae BLYES, S. cerevisiae BLYAS, S. cerevisiae BLYR, were used to assess their reproducibility and utility in screening 68, 69, and 71 chemicals for estrogenic, androgenic, and toxic effects, respectively. EC(50) values were 6.3 +/- 2.4 x 10(-10)M (n = 18) and 1.1 +/- 0.5 x 10(-8)M (n = 13) for BLYES and BLYAS, using 17beta-estradiol and 5alpha-dihydrotestosterone over concentration ranges of 2.5 x 10(-12) through 1.0 x 10(-6)M, respectively. Based on analysis of replicate standard curves and comparison to background controls, a set of quantitative rules have been formulated to interpret data and determine if a chemical is potentially hormonally active, toxic, both, or neither. The results demonstrated that these assays are applicable for Tier I chemical screening in Environmental Protection Agency's Endocrine Disruptor Screening and Testing Program as well as for monitoring endocrine-disrupting activity of unknown chemicals in water.
Sanseverino
John
J
The Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee 37996, USA. jsansev@utk.edu
Eldridge
Melanie L
ML
Layton
Alice C
AC
Easter
James P
JP
Yarbrough
Jason
J
Schultz
Terry Wayne
TW
Sayler
Gary S
GS
D015374:Biosensing Techniques / Q000379:methods*; D003663:Decision Trees; D013196:Dihydrotestosterone / Q000819:agonists* / Q000032:analysis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000819:agonists* / Q000032:analysis / Q000378:metabolism; D017930:Genes, Reporter; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000378:metabolism; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity
D008164:Luminescent Proteins; D013196:Dihydrotestosterone; D004958:Estradiol