Distribution of 14C-TNT and derivatives in different biochemical compartments of Phaseolus vulgaris.
(14)C-TNT was used to quantify the uptake rate and metabolic turnover of TNT in Phaseolus vulgaris. Seventeen plants were analysed by a special cell fractionation method with polar and nonpolar solvents and enzymes. We obtained three cytoplasmic fractions and five cell wall derived fractions. The recovery rate was 72% as measured by liquid scintillation counting. (14)C partitioned almost in equal amounts with approximately 50% in the cytoplasm and in the cell wall. The majority of the TNT-metabolites are present in the cytoplasm as was shown by GC/ECD and thin layer chromatography. The(14)C in the cell wall is bound probably resulting in long-term immobilisation of these metabolites. We conclude that plants may also be a model for nitroaromatic turnover and immobilisation in soil components.
Sens
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Fachgebiet Zellbiologie und Angewandte Botanik, Fachbereich Biologie der Philipps Universität Marburg, D-35032, Marburg, Germany.
Scheidemann
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Klunk
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Werner
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