Major digestive carbohydrase during larval development of meal moth, Plodia interpunctella (Lepidoptera: pyralidae).
The digestive system of P. interpunctella was characterized during its larval development to determination of carbohydrases using disaccharides (sucrose and maltose) and polysaccharides (starch and inulin) as substrate. At 6(th) instar larval, Invertase>alpha-amylase> maltase activities peaks were observed. Invertase was fractionated with acetone and isolated. The Invertase was 485.5 fold purified by Sephacryl S-200 and DEAE-Sephadex. Its kinetic parameters were K(m) of 6.6 mM, V(max) of 0.48, pH optimum of 5.5 and temperature optimum of 30 degrees C. This enzyme was activated by CaCl(2) and inhibited by EDTA. When analyzed by SDS-PAGE it showed one band of M(r) 34 kDa. The understanding of the digestive system of P. interpunctella could be a key step in the design of bioinsecticides.
de Sales
M P
MP
Departamento de Bioquímica, Departamento de Genética e Biologia Celular Centro de Biociências, Universidade Federal do Rio Grande do Norte, Campus Universitário, Natal-RN, Brazil. msales@cb.ufrn.br
Alcazar
Alonso
A
Lima
L M
LM
Amorim
Ticiana M L
TM
Pitanga
J C M
JC
Pereira
R A
RA
Macedo
L L P
LL
Macedo
F P
FP
Oliveira
A S
AS
Uchôa
Adriana F
AF
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D020536:Enzyme Activators / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007814:Larva / Q000201:enzymology / Q000254:growth & development; D009036:Moths / Q000201:enzymology* / Q000254:growth & development; D013696:Temperature; D043324:beta-Fructofuranosidase / Q000302:isolation & purification* / Q000378:metabolism*
D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D006026:Glycoside Hydrolases; C019822:carbohydrase; D043324:beta-Fructofuranosidase