The antithrombotic effects of carrot filtrates in rats and mice.
The prevention of arterial thrombotic disease has a high priority in developed countries. An inappropriate diet is known to enhance the risk for acute thrombotic events and a regular diet with proven antithrombotic effects might be a beneficial way to prevent disease. The present study was undertaken as a part of a series of investigations to examine vegetables and fruits for antithrombotic activity. A shear-induced in vitro platelet reactivity/thrombolysis method [Global Thrombosis Test, formerly Gorog Thrombosis Test] was used to screen 15 different varieties of carrot for antiplatelet and thrombolytic activities. Effective varieties were further investigated using an in vivo, laser-induced thrombosis model in mice. The different carrot varieties demonstrated a variable effect on thrombosis in vitro and in vivo. In particular, a variety designated SAKATA-0421 exerted an antithrombotic effect in vivo independent from heat treatment of the filtrate at 100 degrees C for 10 min. There was no significant correlation between antithrombotic activity and the levels of polyphenolics and any other biochemical parameter, including antioxidant activity, alpha-carotene and beta-carotene, alpha-tocopherol and ascorbic acid. Different varieties of carrot demonstrated a range of antithrombotic and prothrombotic activities. After oral intake, the particular heat resistant variety (SAKATA-0421) showed antithrombotic effect in vivo possibly due to antiplatelet reactivity and/or spontaneous thrombolytic activity. The present study added a new variety to the list of antithrombotic fruits and vegetables.
Yamamoto
Junichiro
J
Kobe Gakuin University, Kobe, Japan. yamamoto@nutr.kobegakuin.ac.jp
Naemura
Aki
A
Ijiri
Yoshinobu
Y
Ogawa
Koji
K
Suzuki
Takao
T
Shimada
Yasuhiko
Y
Giddings
John C
JC
D000818:Animals; D018552:Daucus carota; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D005374:Filtration; D051379:Mice; D008517:Phytotherapy / Q000379:methods*; D010975:Platelet Aggregation Inhibitors; D051381:Rats; D015912:Thrombolytic Therapy / Q000379:methods*; D013927:Thrombosis / Q000178:diet therapy* / Q000517:prevention & control
D005343:Fibrinolytic Agents; D010975:Platelet Aggregation Inhibitors