Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.).
Low-molecular-weight glutenin subunit (LMW-GS) Glu-B3 has a significant influence on the processing quality of the end-use products of common wheat. To characterize the LMW-GS genes at the Glu-B3 locus, gene-specific PCR primers were designed to amplify eight near-isogenic lines and Cheyenne with different Glu-B3 alleles (a, b, c, d, e, f, g, h and i) defined by protein electrophoretic mobility. The complete coding regions of four Glu-B3 genes with complete coding sequence were obtained and designated as GluB3-1, GluB3-2, GluB3-3 and GluB3-4. Ten allele-specific PCR markers designed from the SNPs present in the sequenced variants discriminated the Glu-B3 proteins of electrophoretic mobility alleles a, b, c, d, e, f, g, h and i. These markers were validated on 161 wheat varieties and advanced lines with different Glu-B3 alleles, thus confirming that the markers can be used in marker-assisted breeding for wheat grain processing quality.
Wang
L H
LH
Institute of Crop Science, National Wheat Improvement Centre/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.
Zhao
X L
XL
He
Z H
ZH
Ma
W
W
Appels
R
R
Peña
R J
RJ
Xia
X C
XC
D000483:Alleles; D032461:Chromosomes, Plant; D017343:Genes, Plant; D005819:Genetic Markers; D005983:Glutens / Q000235:genetics*; D006239:Haplotypes; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D021122:Protein Subunits / Q000235:genetics*; D020539:Sequence Analysis, Protein; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
D005819:Genetic Markers; D021122:Protein Subunits; D005983:Glutens; C037302:glutenin