Construction of a binary vector for transformation of Arabidopsis thaliana with a new selection marker.
A new binary vector, pZT4B, containing the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as a selection marker, was constructed. The green fluorescent protein (GFP) gene was inserted into pZT4B, and the resulting plasmid was used in the transformation of Arabidopsis. All of six independent transformants obtained after selection with 0.3 mg/l tunicamycin contained the transgene and showed GFP fluorescence.
Koizumi
Nozomu
N
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Japan.
Iwata
Yuji
Y
D017360:Arabidopsis / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism*; D014170:Transformation, Genetic
D005819:Genetic Markers; D049452:Green Fluorescent Proteins