title: Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice.
abstract: Oxidative stress is implicated in atherogenesis; however most clinical trials with dietary antioxidants failed to show marked success in preventing atherosclerotic diseases. We have found that hydrogen (dihydrogen; H(2)) acts as an effective antioxidant to reduce oxidative stress [I. Ohsawa, M. Ishikawa, K. Takahashi, M. Watanabe, K. Nishimaki, K. Yamagata, K. Katsura, Y. Katayama, S, Asoh, S. Ohta, Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals, Nat. Med. 13 (2007) 688-694]. Here, we investigated whether drinking H(2)-dissolved water at a saturated level (H(2)-water) ad libitum prevents arteriosclerosis using an apolipoprotein E knockout mouse (apoE(-/-)), a model of the spontaneous development of atherosclerosis. ApoE(-/-) mice drank H(2)-water ad libitum from 2 to 6 month old throughout the whole period. Atherosclerotic lesions were significantly reduced by ad libitum drinking of H(2)-water (p=0.0069) as judged by Oil-Red-O staining series of sections of aorta. The oxidative stress level of aorta was decreased. Accumulation of macrophages in atherosclerotic lesions was confirmed. Thus, consumption of H(2)-dissolved water has the potential to prevent arteriosclerosis.
authors.affiliation: Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Nippon Medical School, 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211-8533, Japan.
authors.forename: Ikuroh
authors.initials: I
authors.lastname: Ohsawa
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Nishimaki
authors.forename: Kumi
authors.initials: K
authors.lastname: Yamagata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ohta
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D001011:Aorta / Q000473:pathology; D001057:Apolipoproteins E / Q000235:genetics; D050197:Atherosclerosis / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004326:Drinking; D006859:Hydrogen / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D018345:Mice, Knockout; D018384:Oxidative Stress; D014867:Water / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001057:Apolipoproteins E; D014867:Water; D006859:Hydrogen