Qualitative human immunodeficiency virus RNA analysis of dried blood spots for diagnosis of infections in infants.
The Gen-Probe Aptima human immunodeficiency virus type 1 (HIV-1) RNA assay was adapted for the diagnosis of HIV infection in infants by using dried blood spots. The assay was 99% sensitive (128/129) and 100% specific (162/162). This may prove useful in resource-limited settings, since it precludes the need for a phlebotomist and maintenance of a cold chain.
Kerr
Ryan J S
RJ
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 11-141 Lineberger Cancer Center, CB 7295, Chapel Hill, NC 27599, USA.
Player
Gray
G
Fiscus
Susan A
SA
Nelson
Julie A E
JA
D001769:Blood / Q000821:virology*; D003890:Desiccation; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007223:Infant; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*
D012367:RNA, Viral