Characteristics of a pandemic clone of O3 : K6 and O4 : K68 Vibrio parahaemolyticus isolated in Beira, Mozambique.
The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized diarrhoea patients in Beira, Mozambique, were serotyped for O : K antigens and genotyped for toxR, tdh and trh genes. A group-specific PCR, a PCR that detects the presence of ORF8 of the filamentous phage f237, arbitrarily primed PCR, PFGE and multilocus sequence typing were performed to determine the pandemic status of the strains and their ancestry. All strains of serovars O3 : K6 (n=38) and O4 : K68 (n=4) were identified as a pandemic clonal group by these analyses. These strains are closely related to the pandemic reference strains of O3 : K6 and O4 : K68, which emerged in Asia in 1996 and were later found globally. The pandemic serotypes O3 : K6 and O4 : K68 including reference strains grouped into a single cluster indicating emergence from a common ancestor. The O3 : K58 (n=8), O4 : K13 (n=6), O3 : KUT (n=1) and O8 : K41 (n=1) strains showed unique characteristics different from the pandemic clone.
Ansaruzzaman
M
M
ICDDR,B (International Centre for Diarrhoeal Disease Research, Bangladesh), Mohakhali, Dhaka 1212, Bangladesh.
Chowdhury
Ashrafuzzaman
A
ICDDR,B (International Centre for Diarrhoeal Disease Research, Bangladesh), Mohakhali, Dhaka 1212, Bangladesh.
Bhuiyan
Nurul A
NA
ICDDR,B (International Centre for Diarrhoeal Disease Research, Bangladesh), Mohakhali, Dhaka 1212, Bangladesh.
Sultana
Marzia
M
ICDDR,B (International Centre for Diarrhoeal Disease Research, Bangladesh), Mohakhali, Dhaka 1212, Bangladesh.
Safa
Ashrafus
A
ICDDR,B (International Centre for Diarrhoeal Disease Research, Bangladesh), Mohakhali, Dhaka 1212, Bangladesh.
Lucas
Marcelino
M
Ministry of Health, Maputo, Mozambique.
von Seidlein
Lorenz
L
International Vaccine Institute, Seoul, Republic of Korea.
Barreto
Avertino
A
Ministry of Health, Maputo, Mozambique.
Chaignat
Claire-Lise
CL
World Health Organization, Geneva, Switzerland.
Sack
David A
DA
ICDDR,B (International Centre for Diarrhoeal Disease Research, Bangladesh), Mohakhali, Dhaka 1212, Bangladesh.
Clemens
John D
JD
International Vaccine Institute, Seoul, Republic of Korea.
Nair
G Balakrish
GB
ICDDR,B (International Centre for Diarrhoeal Disease Research, Bangladesh), Mohakhali, Dhaka 1212, Bangladesh.
Choi
Seon Young
SY
School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.|International Vaccine Institute, Seoul, Republic of Korea.
Jeon
Yoon Seong
YS
Interdisciplinary Program of Bioinformatics, Seoul National University, Seoul, Republic of Korea.|School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.|International Vaccine Institute, Seoul, Republic of Korea.
Lee
Je Hee
JH
School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.|International Vaccine Institute, Seoul, Republic of Korea.
Lee
Hye Ri
HR
International Vaccine Institute, Seoul, Republic of Korea.
Chun
Jongsik
J
Interdisciplinary Program of Bioinformatics, Seoul National University, Seoul, Republic of Korea.|School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.|International Vaccine Institute, Seoul, Republic of Korea.
Kim
Dong Wook
DW
International Vaccine Institute, Seoul, Republic of Korea.
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009073:Mozambique / Q000453:epidemiology; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012703:Serotyping; D014735:Vibrio Infections / Q000453:epidemiology* / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
D001426:Bacterial Proteins