Transdentinal diffusion and cytotoxicity of self-etching adhesive systems.
OBJECTIVE
To evaluated the transdentinal diffusion and subsequent cytotoxicity of self-etching adhesives on odontoblast-like cells.


METHODS
Sixty dentin disks (0.4-mm thick) were produced from human molars and divided into six groups (n = 10). The dentin disks were placed in in vitro pulp chambers where MDPC-23 cells were planted on 0.28 cm(2) of exposed dentin on the pulpal side. The adhesives Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Adper Prompt (PR), and Xeno III (XE) were applied on the occlusal side. Single Bond (SB) was used as positive and phosphate buffer solution (PBS) as negative control. The cytotoxicity was measured by MTT assay and cell characteristics were assessed by SEM. The transdentinal diffusion was qualified by GC/MS.


RESULTS
Kruskal-Wallis and Mann-Whitney tests demonstrated a significant difference among the adhesives and PBS. Cellular viability reduction promoted by the self-etching systems was lower than that of SB (53.1%), except for CSE. Cell metabolism was reduced in 47.8%, 42.1%, 28.0%, and 46.5% for CSE, CPB, PR, and XE, respectively. HEMA was identified as the main diffused component.


CONCLUSIONS
Components from all investigated self-etching adhesive systems were able to diffuse through the dentin resulting in significant reduction of the cellular metabolism.
Lanza
Célia Regina Moreira
CR
Department of Oral Clinic, Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Department of Pediatric Dentistry, School of Dentistry, PUC-Minas, Belo Horizonte, MG, Brazil.
de Souza Costa
Carlos Alberto
CA
Furlan
Maysa
M
Alécio
Alberto
A
Hebling
Josimeri
J
D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003804:Dentin / Q000187:drug effects; D003806:Dentin Permeability / Q000187:drug effects*; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D008963:Molar; D009804:Odontoblasts / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D019279:Resin Cements / Q000494:pharmacology; D014014:Tissue Adhesives / Q000009:adverse effects* / Q000032:analysis
C438313:Clearfil SE Bond; D019279:Resin Cements; D014014:Tissue Adhesives; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate