Prefoldin 6 is required for normal microtubule dynamics and organization in Arabidopsis.
Newly translated tubulin molecules undergo a series of complex interactions with nascent chain-binding chaperones, including prefoldin (PFD) and chaperonin-containing TCP-1 (CCT). By screening for oryzalin hypersensitivity, we identified several mutants of Arabidopsis that have lesions in PFD subunits. The pfd6-1 mutant exhibits a range of microtubule defects, including hypersensitivity to oryzalin, defects in cell division, cortical array organization, and microtubule dynamicity. Consistent with phenotypic analysis, proteomic analysis indicates several isoforms of tubulins were reduced in pfd6-1. These results support the concept that the function of microtubules is critically dependent on the absolute amount of tubulins.
Gu
Ying
Y
Department of Plant Biology, Carnegie Institution for Science, 260 Panama Street, Stanford, CA 94305, USA.
Deng
Zhiping
Z
Paredez
Alexander R
AR
DeBolt
Seth
S
Wang
Zhi-Yong
ZY
Somerville
Chris
C
D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D004136:Dinitrobenzenes / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D018546:Hypocotyl / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism; D050505:Mutant Proteins / Q000302:isolation & purification / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D018517:Plant Roots / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects; D017510:Protein Folding / Q000187:drug effects; D040901:Proteomics; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013424:Sulfanilamides / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism
D029681:Arabidopsis Proteins; D004136:Dinitrobenzenes; D018832:Molecular Chaperones; D046912:Multiprotein Complexes; D050505:Mutant Proteins; D013424:Sulfanilamides; D014404:Tubulin; C112806:prefoldin; C533709:prefoldin 6 protein, Arabidopsis; C012465:oryzalin