title: Does selection against transcriptional interference shape retroelement-free regions in mammalian genomes?
abstract: BACKGROUND
  Eukaryotic genomes are scattered with retroelements that proliferate through retrotransposition. Although retroelements make up around 40 percent of the human genome, large regions are found to be completely devoid of retroelements. This has been hypothesised to be a result of genomic regions being intolerant to insertions of retroelements. The inadvertent transcriptional activity of retroelements may affect neighbouring genes, which in turn could be detrimental to an organism. We speculate that such retroelement transcription, or transcriptional interference, is a contributing factor in generating and maintaining retroelement-free regions in the human genome.
  
  
  RESULTS
  Based on the known transcriptional properties of retroelements, we expect long interspersed elements (LINEs) to be able to display a high degree of transcriptional interference. In contrast, we expect short interspersed elements (SINEs) to display very low levels of transcriptional interference. We find that genomic regions devoid of long interspersed elements (LINEs) are enriched for protein-coding genes, but that this is not the case for regions devoid of short interspersed elements (SINEs). This is expected if genes are subject to selection against transcriptional interference. We do not find microRNAs to be associated with genomic regions devoid of either SINEs or LINEs. We further observe an increased relative activity of genes overlapping LINE-free regions during early embryogenesis, where activity of LINEs has been identified previously.
  
  
  CONCLUSIONS
  Our observations are consistent with the notion that selection against transcriptional interference has contributed to the maintenance and/or generation of retroelement-free regions in the human genome.
authors.affiliation: Ancient DNA and Evolution Group, Department of Biology, University of Copenhagen, Copenhagen, Denmark. tmourier@bi.ku.dk
authors.forename: Tobias
authors.initials: T
authors.lastname: Mourier
authors.forename: Eske
authors.initials: E
authors.lastname: Willerslev
mesh_terms: D000818:Animals; D003198:Computer Simulation; D005801:Genes, Homeobox; D005823:Genetics; D016678:Genome; D015894:Genome, Human; D006801:Humans; D020084:Long Interspersed Nucleotide Elements; D008322:Mammals / Q000235:genetics; D051379:Mice; D035683:MicroRNAs; D008957:Models, Genetic; D018626:Retroelements; D020086:Short Interspersed Nucleotide Elements; D014158:Transcription, Genetic
chemical_list: D035683:MicroRNAs; D018626:Retroelements