A microfluidic device with groove patterns for studying cellular behavior.
We describe a microfluidic device with microgrooved patterns for studying cellular behavior. This microfluidic platform consists of a top fluidic channel and a bottom microgrooved substrate. To fabricate the microgrooved channels, a top poly(dimethylsiloxane) (PDMS) mold containing the impression of the microfluidic channels was aligned and bonded to a microgrooved substrate. Using this device, mouse fibroblast cells were immobilized and patterned within microgrooved substrates (25, 50, 75, and 100 microm wide). To study apoptosis in a microfluidic device, media containing hydrogen peroxide, Annexin V, and propidium iodide was perfused into the fluidic channel for 2 hours. We found that cells exposed to the oxidative stress became apoptotic. These apoptotic cells were confirmed by Annexin V that bound to phosphatidylserine at the outer leaflet of the plasma membrane during the apoptosis process. Using this microfluidic device with microgrooved patterns, the apoptosis process was observed in real-time and analyzed by using an inverted microscope containing an incubation chamber (37 degrees C, 5% CO(2)). Therefore, this microfluidic device incorporated with microgrooved substrates could be useful for studying the cellular behavior and performing high-throughput drug screening.
Chung
Bong Geun
BG
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.
Manbachi
Amir
A
Khademhosseini
Ali
A
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology; D002468:Cell Physiological Phenomena; D002477:Cells / Q000187:drug effects / Q000378:metabolism; D004867:Equipment Design; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D041681:NIH 3T3 Cells; D016877:Oxidants / Q000494:pharmacology; D011419:Propidium / Q000378:metabolism
D017304:Annexin A5; D016877:Oxidants; D011419:Propidium; D006861:Hydrogen Peroxide