Improved production of raw starch degrading enzyme by Aspergillus oryzae F-30 using methyl glucoside sesqui-stearate.
The effect of methyl glucoside sesqui-stearate (MGS) on the production of raw starch degrading enzyme (RSDE) by Aspergillus oryzae F-30 was studied in this paper. The activity of RSDE formed by Aspergillus oryzae F-30 was enhanced dramatically by the addition of MGS to the medium. As a result, with the addition of 1.5 g MGS in 1 L basal medium, RSDE activity and productivity were 107 U mL(-1) and 1.49 U mL(-1) h(-1), 4.3-fold and 7.1-fold greater than the values obtained in the basal medium, respectively. The effect of MGS on the synthesis of RSDE by Aspergillus oryzae F-30 was also studied on a molecular level. It was observed that the regulation of RSDE synthesis in Aspergillus oryzae F-30 occurs at both transcriptional and translational level and the enzyme synthesis was provoked by the addition of MGS at transcriptional level.
Sun
Hai-Yan
HY
State Key Laboratory of Tropical Corp Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Hainan, China.
Wang
Lu
L
Liu
Jian-Wen
JW
Peng
Ming
M
D001236:Aspergillus oryzae / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000502:physiology; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry*; D013045:Species Specificity; D013213:Starch / Q000737:chemistry* / Q000378:metabolism*; D013229:Stearic Acids / Q000494:pharmacology*
D013229:Stearic Acids; D013213:Starch; D008049:Lipase