title: Analysis of single, purified inclusions as a novel approach to understand methamphetamine neurotoxicity.
abstract: A variety of neurodegenerative diseases leading to movement disorders such as Parkinson's disease (PD) are characterized by neuronal inclusions. Despite evidence of the presence of these intrusions, these intracellular bodies have been poorly investigated because of the technical limits of reproducing them in experimental models and the difficulties in isolating these ultrastuctures. Here, we describe a simple method for the isolation of single, purified inclusion bodies using immunomagnetic separation. We profited from the high number and maturation stage of inclusions produced in vitro by methamphetamine (METH) in cultured PC12 cells; in fact, this experimental condition is highly reproducible and has a limited number of experimental variables, while it is predictive of what is described in vivo in dopamine neurons.
authors.affiliation: Department of Human Morphology and Applied Biology, University of Pisa, Pisa, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Lenzi
authors.forename: Federica
authors.initials: F
authors.lastname: Fulceri
authors.forename: Gloria
authors.initials: G
authors.lastname: Lazzeri
authors.forename: Alessandro
authors.initials: A
authors.lastname: Casini
authors.forename: Stefano
authors.initials: S
authors.lastname: Ruggieri
authors.forename: Antonio
authors.initials: A
authors.lastname: Paparelli
authors.forename: Francesco
authors.initials: F
authors.lastname: Fornai
mesh_terms: D000818:Animals; D015259:Dopamine Agents / Q000633:toxicity*; D006801:Humans; D018189:Immunomagnetic Separation; D002479:Inclusion Bodies / Q000737:chemistry* / Q000648:ultrastructure; D008694:Methamphetamine / Q000633:toxicity*; D016716:PC12 Cells; D020543:Proteome / Q000032:analysis; D051381:Rats
chemical_list: D015259:Dopamine Agents; D020543:Proteome; D008694:Methamphetamine