title: Purification, crystallization and data collection of Pectobacterium chrysanthemi AcsD, a type A siderophore synthetase.
abstract: AcsD, a type A siderophore synthetase with a molecular weight of 71 140 Da from Pectobacterium chrysanthemi, has been expressed, purified and crystallized at 293 K. The protein crystallized in the primitive orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 80.3, b = 95.7, c = 161.1 A, alpha = beta = gamma = 90 degrees . Systematic absences were consistent with space group P2(1)2(1)2(1). A complete data set has been collected to 2.25 A resolution on BM14 at the ESRF. Consideration of the likely solvent content suggested that the asymmetric unit contained two molecules. Gel-filtration experiments indicated that the protein was a dimer, although self-rotation analyses did not detect a convincing twofold symmetry axis in the asymmetric unit. The protein has no convincing sequence match to any known structure and thus solution is likely to require experimental phasing.
authors.affiliation: Scottish Structural Proteomics Facility and Centre for Biomolecular Sciences, The University, St Andrews, Fife KY16 9ST, Scotland.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: McMahon
authors.forename: Muse
authors.initials: M
authors.lastname: Oke
authors.forename: Huanting
authors.initials: H
authors.lastname: Liu
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Johnson
authors.forename: Lester
authors.initials: L
authors.lastname: Carter
authors.forename: Nadia
authors.initials: N
authors.lastname: Kadi
authors.forename: Malcolm F
authors.initials: MF
authors.lastname: White
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Challis
authors.forename: James H
authors.initials: JH
authors.lastname: Naismith
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D016974:Dickeya chrysanthemi / Q000201:enzymology*; D008025:Ligases / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017262:Siderophores / Q000096:biosynthesis*; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D017262:Siderophores; D008025:Ligases