title: Evaluation of a gel-permeation high-performance liquid chromatography for determining triglyceride levels in serum major lipoproteins, compared with the ultracentrifugation/precipitation method.
abstract: OBJECTIVE
  To evaluate the gel permeation high-performance liquid chromatography (GP-HPLC) method for determination of triglyceride (TG) levels in low-density lipoprotein (LDL) and high-density lipoprotein (HDL).
  
  
  METHODS
  The GP-HPLC and the ultracentrifugation (UC)/precipitation methods were used and compared.
  
  
  RESULTS
  There was no significant difference in measured levels of LDL-triglyceride between the two methods, but the HDL-triglyceride levels measured by the GP-HPLC technique were significantly higher than the UC/precipitation one (145+/-47 mg/L and 121+/-45 mg/L respectively, n=38, p<0.0001).
  
  
  CONCLUSIONS
  A GP-HPLC technique provides LDL-TG levels comparable to those obtained by the UC/precipitation method.
authors.affiliation: Department of Medical Technology, Okayama University Graduate School of Health Sciences, Okayama, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Usui
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizuno
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Okazaki
authors.forename: Masakazu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Ikunosuke
authors.initials: I
authors.lastname: Sakurabayashi
mesh_terms: D011232:Chemical Precipitation; D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D015203:Reproducibility of Results; D014280:Triglycerides / Q000097:blood*; D014461:Ultracentrifugation / Q000379:methods*
chemical_list: C034396:HDL-triglyceride; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D014280:Triglycerides