Isolation and structural elucidation of polar photometabolites.
Subsequent to irradiation with a xenon lamp simulating sunlight, fluoroquinolone carboxylic acids in aqueous solution form polar pyridone dicarboxylic and tricarboxylic acids. After liquid/liquid partition with chloroform/water these substances can be isolated by ion exchange chromatography of the aqueous phase. They can be regarded as intermediate compounds on the route to a complete photomineralization. The structural elucidation is performed by such mass spectroscopic methods as MS, GC/MS and HPLC/MS, whereby HPLC/MS shows the highest reliability. Additionally(1)H- and(13)C-NMR measurements confirm the structure of the main polar degradation product.
Burhenne
J
J
Department of Ecological Chemistry and Ecotoxicology, University of Kassel, Nordbahnhofstrasse la, D-37213, Witzenhausen, Germany.
Ludwig
M
M
Spiteller
M
M