Prevalence and dissemination of the Ser315Thr substitution within the KatG enzyme in isoniazid-resistant strains of Mycobacterium tuberculosis isolated in Uruguay.
The aim of this study was to determine the prevalence of Ser315Thr substitution in isoniazid (INH)-resistant strains of Mycobacterium tuberculosis in Uruguay. The katG gene of 62 INH-resistant strains was analysed by an RFLP-PCR assay. PCR products were digested with MspI to detect Ser315Thr and Arg463Leu substitutions. A total of 16 of the 62 (26 %) INH-resistant strains analysed had a Ser315Thr substitution. Only one INH-resistant strain had an Arg463Leu substitution and two strains had a deletion in katG. Of the 16 strains with Ser315Thr, 15 showed different profiles using a double-repetitive-element PCR assay, demonstrating that there was no local dissemination of any particular strain. These findings are in agreement with published data from regions where the prevalence of tuberculosis (TB) is intermediate and may be due in part to the success of the local TB control programme.
Varela
Gustavo
G
Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
González
Sabina
S
Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
Gadea
Pilar
P
Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
Coitinho
Cecilia
C
Laboratory Department, Honorary Anti-Tuberculosis Commission, 18 de Julio 2175, Montevideo, Uruguay.
Mota
Inés
I
Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
González
Gladys
G
Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
Goñi
Fernando
F
Department of Neurology, New York University School of Medicine, New York, USA.|Department of Immunology, School of Chemistry, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
Rivas
Carlos
C
Laboratory Department, Honorary Anti-Tuberculosis Commission, 18 de Julio 2175, Montevideo, Uruguay.
Schelotto
Felipe
F
Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
D019943:Amino Acid Substitution; D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002374:Catalase / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000382:microbiology; D014583:Uruguay / Q000453:epidemiology
D000995:Antitubercular Agents; D001426:Bacterial Proteins; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; D007538:Isoniazid