Proteomic map of Trypanosoma cruzi CL Brener: the reference strain of the genome project.
In this work two-dimensional gel electrophoresis combined with mass spectrometry was carried out in order to start the construction of a map of soluble proteins from epimastigote form of Trypanosoma cruzi CL Brener. This strain is a hybrid organism derived from two genotypes, T. cruzi I and T. cruzi II and was chosen for genome sequencing. The two-dimensional gel electrophoresis showed that most of proteins focused at 4-7 pH range. The identification demonstrated that several proteins were in multiple isoforms, such as tubulin and heat shock proteins. Potential targets for development of chemotherapeutic agents like arginine kinase, an enzyme absent from mammalian tissues that is involved in the energy supply of the parasite, were also detected.
Sodré
Cátia Lacerda
CL
Laboratory of Molecular Epidemiology of Infectious Diseases, Oswaldo Cruz Institute-IOC, Oswaldo Cruz Foundation-FIOCRUZ. Av. Brasil, 4365-Manguinhos, Rio de Janeiro, RJ 21045-900, Brazi. csodre@fiocruz.br
Chapeaurouge
Alexander D
AD
Kalume
Dário Eluan
DE
de Mendonça Lima
Leila
L
Perales
Jonas
J
Fernandes
Octavio
O
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D018503:Genome, Protozoan; D008969:Molecular Sequence Data; D040901:Proteomics; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D015800:Protozoan Proteins