Generation of mouse-human hybridomas secreting antibodies against peanut allergen Ara h1.
Two clones of mouse-human hybridomas, secreting human monoclonal antibodies to a peanut allergen Ara h1, were generated from human peripheral blood lymphocytes transformed with Epstein--Barr virus, followed by cell fusion with mouse myeloma cells. Epitope analysis with overlapping peptides synthesized on a multi-pin apparatus revealed antibody-binding sequences of Ara h1 protein.
Shinmoto
Hiroshi
H
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan, shinmoto@affrc.go.jp.
Naganawa
Yasunori
Y
Shimmoto
Michie
M
Maleki
Soheila J
SJ