title: Chaga mushroom extract inhibits oxidative DNA damage in lymphocytes of patients with inflammatory bowel disease.
abstract: Inflammatory Bowel Disease (IBD) is partly caused by oxidative stress from free radicals and reduced antioxidant levels. Using hydrogen peroxide to induce oxidative stress in vitro in peripheral lymphocytes we investigated the induction of DNA damage supplemented with ethanolic extract of Chaga mushroom as a protective antioxidant. Lymphocytes were obtained from 20 IBD patients and 20 healthy volunteers. For treatment, a constant H_{2}O_{2 } dose (50 microg/ml) was used with variable doses of Chaga extract (10-500 microg/ml). DNA damage was evaluated in 50 cells per individual and dose using the Comet assay (making 1000 observations per experimental point ensuring appropriate statistical power). Chaga supplementation resulted in a 54.9% (p < 0.001) reduction of H_{2}O_{2 } induced DNA damage within the patient group and 34.9% (p < 0.001) within the control group. Lymphocytes from Crohn's disease (CD) patients had a greater basic DNA damage than Ulcerative Colitis (UC) patients (p < 0.001). Conclusively, Chaga extract reduces oxidative stress in lymphocytes from IBD patients and also healthy individuals when challenged in vitro. Thus, Chaga extract could be a possible and valuable supplement to inhibit oxidative stress in general.
authors.affiliation: Division of Biomedical Sciences, University of Bradford, Bradford, UK.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Najafzadeh
authors.forename: P Dominic
authors.initials: PD
authors.lastname: Reynolds
authors.forename: Adolf
authors.initials: A
authors.lastname: Baumgartner
authors.forename: David
authors.initials: D
authors.lastname: Jerwood
authors.forename: Diana
authors.initials: D
authors.lastname: Anderson
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D001688:Biological Products