Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein.
Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA(306-318)) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria.
Saravia
Carolina
C
Fundación Instituto de Inmunología de Colombia, Molecular Biology, Carrera 50#26-20, Bogota, Colombia.
Martinez
Paola
P
Granados
Diana S
DS
Lopez
Carolina
C
Reyes
Claudia
C
Patarroyo
Manuel A
MA
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018604:Epitope Mapping; D006684:HLA-DR Antigens / Q000276:immunology; D059811:HLA-DRB1 Chains; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D017780:Malaria Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000276:immunology; D010966:Plasmodium vivax / Q000276:immunology*; D011487:Protein Conformation; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016415:Sequence Alignment; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000953:Antigens, Protozoan; C066001:Duffy antigen binding protein, Plasmodium; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D016056:Immunodominant Epitopes; D017780:Malaria Vaccines; D010455:Peptides; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D014614:Vaccines, Synthetic