HIV-1 and HIV-2 produce different amounts of 2-long terminal repeat circular DNA in vitro.
We assessed HIV-1 and HIV-2 2-long terminal repeat (LTR) circular DNA production in peripheral blood mononuclear cells, MT4-CXCR4 cells and HeLa-CXCR4-CCR5 cells in vitro, relative to the respective total amounts of HIV DNA. Whatever the cell type, HIV-2 produced a smaller total amount of DNA than HIV-1 between 6 and 96 h; HIV-2 2-LTR DNA appeared later than HIV-1 2-LTR DNA, but rapidly became more abundant. This accumulation of HIV-2 2-LTR DNA points to less efficient host cell integration relative to HIV-1.
Gueudin
Marie
M
Laboratoire de virologie UPRES EA 2656 - Laboratoire associé du centre de référence pour VIH, CHU Charles Nicolle 76031 ROUEN Cedex, France. Marie.Gueudin@chu-rouen.fr
Braun
Joséphine
J
Plantier
Jean-Christophe
JC
Simon
François
F
D018791:CD4 Lymphocyte Count; D004270:DNA, Circular / Q000235:genetics*; D015658:HIV Infections / Q000235:genetics* / Q000821:virology; D016325:HIV Long Terminal Repeat / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D015498:HIV-2 / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics
D004270:DNA, Circular; D012367:RNA, Viral