title: Comparative transcriptome analysis of arsenate and arsenite stresses in rice seedlings.
abstract: The effect of arsenic (As) exposure on genome-wide expression was examined in rice (Oryza sativa L., ssp. Indica). A group of defense and stress-responsive genes, transporters, heat-shock proteins, metallothioneins, sulfate-metabolizing proteins, and regulatory genes showed differential expression in rice seedlings challenged with arsenate (AsV) and arsenite (AsIII). AsV stress led to upregulation or downregulation of an additional set of genes in comparison to AsIII. Differential expression of several genes that showed the highest contrast in a microarray analysis was validated by following the quantitative changes in the levels of individual transcripts following challenge with AsV, AsIII, Cd, Cr, and Pb. Most of the selected genes responded to challenge by heavy metals such as arsenic. However, expression of one of the cytochrome P450 genes (Os01g43740) in rice root was induced by AsV but not by other heavy metals. Similarly, one glutaredoxin (Os01g26912) is expressed specifically in the AsIII-treated shoot.
authors.affiliation: National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, UP, India.
authors.forename: Debasis
authors.initials: D
authors.lastname: Chakrabarty
authors.forename: Prabodh Kumar
authors.initials: PK
authors.lastname: Trivedi
authors.forename: Prashant
authors.initials: P
authors.lastname: Misra
authors.forename: Manish
authors.initials: M
authors.lastname: Tiwari
authors.forename: Manju
authors.initials: M
authors.lastname: Shri
authors.forename: Devesh
authors.initials: D
authors.lastname: Shukla
authors.forename: Smita
authors.initials: S
authors.lastname: Kumar
authors.forename: Arti
authors.initials: A
authors.lastname: Rai
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Pandey
authors.forename: Deepti
authors.initials: D
authors.lastname: Nigam
authors.forename: Rudra Dev
authors.initials: RD
authors.lastname: Tripathi
authors.forename: Rakesh
authors.initials: R
authors.lastname: Tuli
mesh_terms: D001149:Arsenates / Q000494:pharmacology*; D018053:Arsenites / Q000494:pharmacology*; D020869:Gene Expression Profiling; D017343:Genes, Plant; D018745:Genome, Plant; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological
chemical_list: D001149:Arsenates; D018053:Arsenites; D006360:Heat-Shock Proteins; D006540:Herbicides; D010940:Plant Proteins; C015001:arsenite; C025657:arsenic acid