Immersion oil for high-resolution live-cell imaging at 37 degrees C: optical and physical characteristics.
The use of normal immersion oil, developed for 23 degrees C, at 37 degrees C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37 degrees C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23 degrees C counterparts.
Oomen
L C J M
LC
Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. l.oomen@nki.nl
Sacher
R
R
Brocks
H H J
HH
Zwier
J M
JM
Brakenhoff
G J
GJ
Jalink
K
K
D000818:Animals; D002478:Cells, Cultured; D007668:Kidney / Q000166:cytology; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009821:Oils; D013696:Temperature
D009821:Oils