Inhibition effect of pcDNA-tum-5 on the growth of S180 tumor.
Tumor growth and metastasis depend on vessel formation, and inhibition of angiogenesis of tumor by production of anti-angiogenic drugs should be a promising approach for cancer therapy. Tumstatin is an angiogenesis inhibitor. The anti-angiogenic activity of tumstatin is localized to the 54-132 amino acids. The gene fragment encoding amino acids 45-132 of tumstatin (tum-5) was subcloned into pcDNA3.1 (pcDNA-tum5). Tum-5 protein could be expressed and secreted in CHO cells after transfection. The conditioned medium (containing tum-5 protein) from the transfectant has an anti-angiogenic effect on HUVEC cells in vitro. The anti-tumor effect of pcDNA-tum5 on mice bearing S180 tumors was evaluated. The results showed that pcDNA-tum-5 has a significant inhibition activity in the growth of the tumors. This study suggests that the gene delivery of tum-5 may be an effective strategy for cancer therapy.
You
Yanjie
Y
Biotechnology Center of The Fourth Military Medical University, 17 Changle West Road, 710032, Xi'an, People's Republic of China.
Xue
Xiaochang
X
Li
Meng
M
Qin
Xin
X
Zhang
Cun
C
Wang
Weihua
W
Giang
Changli
C
Wu
Shouzhen
S
Liu
Yan
Y
Zhu
Wenhua
W
Ran
Yonggang
Y
Zhang
Zhen
Z
Han
Wei
W
Zhang
Yingqi
Y