title: Synergy of gatifloxacin with cefoperazone and cefoperazone-sulbactam against resistant strains of Pseudomonas aeruginosa.
abstract: Chequerboard and time-kill methods were used to compare the in vitro efficacies of the combinations gatifloxacin (GAT) with cefoperazone (CFP) and GAT with cefoperazone-sulbactam (CFP-SUL) against 58 clinical isolates of Pseudomonas aeruginosa. The combinations GAT+CFP and GAT+CFP-SUL were shown to be synergistic for 36.2 and 58.6 % of isolates tested, respectively, using the chequerboard method. Time-kill studies with 11 strains showed synergy in 54.5 % for the GAT+CFP combination and 72.7 % for the GAT+CFP-SUL combination. The agreement between these two methods was found to be 72-81 %. There was a significant difference in synergy between the two combinations tested (P=0.011).
authors.affiliation: Department of Pharmaceutical Biotechnology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, India.|Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India.
authors.forename: N
authors.initials: N
authors.lastname: Sivagurunathan
authors.affiliation: Department of Pharmaceutical Biotechnology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, India.
authors.forename: S
authors.initials: S
authors.lastname: Krishnan
authors.affiliation: Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India.
authors.forename: J Venkat
authors.initials: JV
authors.lastname: Rao
authors.affiliation: Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India.
authors.forename: Anantha Naik
authors.initials: AN
authors.lastname: Nagappa
authors.affiliation: Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India.
authors.forename: V M
authors.initials: VM
authors.lastname: Subrahmanyam
authors.affiliation: Department of Pharmaceutical Biotechnology, Shree Devi College of Pharmacy, Airport Road, Kenjar Village, Mangalore, Karnataka 574142, India.
authors.forename: B Meenashi
authors.initials: BM
authors.lastname: Vanathi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002438:Cefoperazone / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D024841:Fluoroquinolones / Q000494:pharmacology*; D000077734:Gatifloxacin; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D013407:Sulbactam / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D002438:Cefoperazone; D000077734:Gatifloxacin; D013407:Sulbactam