Manipulation of mammalian cells using a single-fiber optical microbeam.
The short working distance of microscope objectives has severely restricted the application of optical micromanipulation techniques at larger depths. We show the first use of fiber-optic tweezers toward controlled guidance of neuronal growth cones and stretching of neurons. Further, by mode locking, the fiber-optic tweezers beam was converted to fiber-optic scissors, enabling dissection of neuronal processes and thus allowing study of the subsequent response of neurons to localized injury. At high average powers, lysis of a three-dimensionally trapped cell was accomplished.
Mohanty
Samarendra K
SK
University of California-Irvine, Beckman Laser Institute, Irvine, California 92612, USA. smohanty@uci.edu
Mohanty
Khyati S
KS
Berns
Michael W
MW
D000818:Animals; D016466:CHO Cells; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D017076:Computer-Aided Design; D006224:Cricetinae; D003412:Cricetulus; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053685:Laser Therapy / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D008866:Microsurgery / Q000295:instrumentation*; D052898:Optical Tweezers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity