[Study of immunogenic properties of recombinant outer membrane proteins F and L from Pseudomonas aeruginosa].
Recombinant outer membrane proteins OprF, OprL, and C-terminal part of OprF protein (OprF(192-342)) was synthesized in Escherichia coil M15 cells. It was shown that in rabbits immunized with chromatographically purified recombinant proteins mounted specific antibody response. In experiments in vitro obtained hyperimmune to OprF, OprL, and OprF(192-342) rabbit sera inhibited growth of P. aeruginosa culture and in experiments in vivo protected mice from infection caused by this microorganism.
Kaloshin
A A
AA
Zlygostev
S A
SA
Gatypova
E V
EV
Mikhaĭlova
N A
NA
D000818:Animals; D000918:Antibody Specificity; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D002845:Chromatography; D004926:Escherichia coli / Q000235:genetics; D007106:Immune Sera / Q000276:immunology; D007274:Injections, Intraperitoneal; D051379:Mice; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
D001425:Bacterial Outer Membrane Proteins; D007106:Immune Sera; D011994:Recombinant Proteins