title: UDP-Gal: N-acetylglucosamine beta 1-4 galactosyltransferase expressing live attenuated parasites as vaccine for visceral leishmaniasis.
abstract: As compared to cutaneous leishmaniasis, vaccination against visceral leishmaniasis (VL) has received limited attention. In this study, we demonstrate for the first time that an UDP-Galactose: N-acetylglucosamine beta 1-4 galactosyltransferase (GenBank Accession No. EF159943) expressing attenuated LD clonal population (A-LD) is able to confer protection against the experimental challenge with the virulent LD AG83 parasite. A-LD was also effective in established leishmania infection. The vaccinated animals showed both cell mediated (in vitro T-cell proliferation, and DTH response) and humoral responses (Th1 type). These results demonstrate the potential of the attenuated clones as an immunotherapeutic and immunoprophylactic agent against visceral leishmaniasis.
authors.affiliation: Division of Infectious Disease and Immunology, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Kolkata, 700032, India.
authors.forename: Siddhartha Kumar
authors.initials: SK
authors.lastname: Bhaumik
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Singh
authors.forename: Subir
authors.initials: S
authors.lastname: Karmakar
authors.forename: Tripti
authors.initials: T
authors.lastname: De
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology; D005700:Galactosyltransferases / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007728:Kupffer Cells / Q000378:metabolism / Q000469:parasitology; D007893:Leishmania donovani / Q000201:enzymology* / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000517:prevention & control*; D008099:Liver / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D016052:Protozoan Vaccines / Q000276:immunology*; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000469:parasitology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D016052:Protozoan Vaccines; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D005700:Galactosyltransferases