title: Effect of variation in RANTES promoter on serum RANTES levels and risk of recurrent wheezing after RSV bronchiolitis in children from Han, Southern China.
abstract: To investigate the association among RANTES (regulated on activation normal T cell expressed and secreted) gene promoter polymorphism, serum RANTES levels, and recurrent wheezing after RSV (respiratory syncytial virus) bronchiolitis in children (1-12 months of age) from Han, Southern china. Three hundred twenty children with RSV bronchiolitis and 272 controls were enrolled in the 3-year follow-up study. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP), enzyme-linked immunosorbent assay (ELISA) kit and luciferase analysis were the mainly used methods, which were used to genotype the RANTES (-403 G/A), assess the serum RANTES levels and the RANTES promoter activity. As the results showed, the RANTES (-403 G/A) in the promoter region was associated with recurrent wheezing after RSV bronchiolitis (p < 0.05) and serum RANTES levels (RANTES genotype G/G: 26.03 +/- 7.46 ng/ml G/A: 28.22 +/- 6.44 ng/ml A/A: 30.12 +/- 5.88 ng/ml). Functional analyses of RANTES promoter activity indicated that the RANTES (-403 G to A) mutation increases the transcriptional activity of the RANTES promoter. In conclusion, the RANTES (-403 G/A) polymorphism increases RANTES transcriptional activity resulted in a high serum RANTES levels, thus increased the risk of recurrent wheezing after RSV bronchiolitis.
authors.affiliation: Department of Respiratory Medicine, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing, China.
authors.forename: Man
authors.initials: M
authors.lastname: Tian
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Guan-yu
authors.initials: GY
authors.lastname: Wen
authors.forename: Sheng-yun
authors.initials: SY
authors.lastname: Shi
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Chen
authors.forename: De-yu
authors.initials: DY
authors.lastname: Zhao
mesh_terms: D044466:Asian People / Q000235:genetics; D001249:Asthma / Q000453:epidemiology / Q000235:genetics / Q000276:immunology / Q000821:virology; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000097:blood* / Q000235:genetics*; D002681:China / Q000453:epidemiology; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008297:Male; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D012008:Recurrence; D012135:Respiratory Sounds / Q000235:genetics* / Q000276:immunology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*
chemical_list: D018946:Chemokine CCL5