title: EdU, a new thymidine analogue for labelling proliferating cells in the nervous system.
abstract: Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a covalent bond via the "click" chemistry reaction (the Huisgen 1,3-dipolar cycloaddition reaction of an organic azide to a terminal acetylene). Unlike the commonly used BrdU, EdU detection requires no heat or acid treatment. It is quick and easy and compatible with multiple probes for fluorescence immunochemistry, facilitating the characterisation of proliferating cells at high resolution.
authors.affiliation: National Centre for Adult Stem Cell Research, Eskitis Institute for Cellular & Molecular Therapies, Griffith University, Brisbane, Qld 4111, Australia. f.chehrehasa@griffith.edu.au
authors.forename: Fatemah
authors.initials: F
authors.lastname: Chehrehasa
authors.forename: Adrian C B
authors.initials: AC
authors.lastname: Meedeniya
authors.forename: Patrick
authors.initials: P
authors.lastname: Dwyer
authors.forename: Greger
authors.initials: G
authors.lastname: Abrahamsen
authors.forename: Alan
authors.initials: A
authors.lastname: Mackay-Sim
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002461:Cell Line, Transformed; D004305:Dose-Response Relationship, Drug; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007211:Indoles; D051379:Mice; D008810:Mice, Inbred C57BL; D009420:Nervous System / Q000166:cytology* / Q000187:drug effects; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010671:Phenylurea Compounds / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D011247:Pregnancy; D013936:Thymidine / Q000378:metabolism
chemical_list: D000199:Actins; D005904:Glial Fibrillary Acidic Protein; D007211:Indoles; D010671:Phenylurea Compounds; C007293:DAPI; C032766:ethylene diurea; D001973:Bromodeoxyuridine; D013936:Thymidine