Production of interferon-beta by NB1-RGB cells cultured on peptide-lipid membranes.
Cell growth and production of interferon-beta (IFN-beta) were investigated for normal human skin fibroblast cells (NB1-RGB) cultured on membranes prepared from peptide-lipids containing the arginine-glycine-aspartic acid [Arg-Gly-Asp] (RGD), tyrosine-isoleucine-glycine-serine-arginine [Tyr-Ile-Gly-Ser-Arg] (YIGSR) and arginine-glutamic acid-aspartic acid-valine [Arg-Glu-Asp-Val] (REDV) peptides. Cell density was found to be approximately the same on various peptide-lipid membranes, whereas production of IFN-beta depended significantly on the peptide-lipid membranes on which NB1-RGB cells were cultured. The highest production of IFN-beta was observed for NB1-RGB cells on REDV-lipid membranes prepared by a casting method (REDV-cast membranes) after 24 hr of cultivation. Specific binding between REDV of REDV-cast membranes and the receptor on the NB1-RGB cells may have caused the specific cell response for the production of IFN-beta.
Hara
Mariko
M
Department of Applied Chemistry, Seikei University, 3-1 Kichijoji Kitamachi 3, Musashino, 180-8633, Japan.
Takanashi
Yasunari
Y
Tuzuki
Nobuya
N
Kawakami
Hiroko
H
Toma
Kazunori
K
Higuchi
Akon
A