The concordance test emerges as a powerful tool for identifying quantitative trait nucleotides: lessons from BTA6 milk yield QTL.
The lack of conventions for confirming the discovery of quantitative trait nucleotides in livestock was evidenced by the proposals of mutations in two different genes (SPP1 and ABCG2) as the underlying functional mutation for a major quantitative trait locus (QTL) for milk concentration on bovine chromosome 6 (BTA6). Of these conflicting candidates, SPP1 was excluded by follow-up studies and by the data described here. A simple test for concordance of the zygosity state between QTL segregation status and the candidate polymorphism was shown, in this case, to be a critical step towards establishing the proof. If a given sample effectively represents the genetic variation across the QTL region, haplotype-based concordance may further enhance the functionality and resolution power of this test, allowing identification of the causative gene.
Seroussi
E
E
Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel. seroussi@agri.huji.ac.il
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005821:Genetic Techniques; D006239:Haplotypes; D007774:Lactation; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D008892:Milk / Q000378:metabolism*; D008957:Models, Genetic; D009154:Mutation; D053495:Osteopontin / Q000235:genetics; D040641:Quantitative Trait Loci
D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D053495:Osteopontin