title: The in vivo stability of electrospun polycaprolactone-collagen scaffolds in vascular reconstruction.
abstract: To avoid complications of prosthetic vascular grafts, engineered vascular constructs have been investigated as an alternative for vascular reconstruction. The scaffolds for vascular tissue engineering remain a cornerstone of these efforts and yet many currently available options are limited by issues of inconsistency, poor adherence of vascular cells, or inadequate biomechanical properties. In this study, we investigated whether PCL/collagen scaffolds could support cell growth and withstand physiologic conditions while maintaining patency in a rabbit aortoiliac bypass model. Our results indicate that electrospun scaffolds support adherence and growth of vascular cells under physiologic conditions and that endothelialized grafts resisted adherence of platelets when exposed to blood. When implanted in vivo, these scaffolds were able to retain their structural integrity over 1 month of implantation as demonstrated by serial ultrasonography. Further, at retrieval, these scaffolds continued to maintain biomechanical strength that was comparable to native artery. This study suggests that electrospun scaffolds combined with vascular cells may become an alternative to prosthetic vascular grafts for vascular reconstruction.
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Winston-Salem, NC 27157, USA. btillman@wfubmc.edu
authors.forename: Bryan W
authors.initials: BW
authors.lastname: Tillman
authors.forename: Saami K
authors.initials: SK
authors.lastname: Yazdani
authors.forename: Sang Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Randolph L
authors.initials: RL
authors.lastname: Geary
authors.forename: Anthony
authors.initials: A
authors.lastname: Atala
authors.forename: James J
authors.initials: JJ
authors.lastname: Yoo
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000378:metabolism*; D002448:Cell Adhesion; D003094:Collagen / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000648:ultrastructure; D008422:Materials Testing; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D010973:Platelet Adhesiveness; D011091:Polyesters / Q000378:metabolism*; D019651:Plastic Surgery Procedures; D012756:Sheep; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D003094:Collagen