Expression, cellular localization and antibody responses of the African swine fever virus genes B602L and K205R.
Previously, we identified serological immunodeterminants of African swine fever virus (ASFV), including pK205R and pB602L, without homologues in the database. pK205R is expressed as a 33-kD protein from 4 h post-infection onward, initially diffusely distributed throughout cells, and subsequently in viral factories. pK205R was not found in purified virus. Both pK205R and pB602L are recognised by hyperimmune antisera from domestic pigs and bushpigs at late time points after infection, suggesting they may be useful diagnostically to distinguish animals persistently infected with virus.
Gutiérrez-Castañeda
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Pirbright Laboratory, Institute for Animal Health, Ash Road, Pirbright, Woking, Surrey, GU240NF, UK.
Reis
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Corteyn
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A
Parkhouse
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RM
Kollnberger
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S
D000357:African Swine Fever / Q000097:blood / Q000175:diagnosis; D000358:African Swine Fever Virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D002522:Chlorocebus aethiops; D015870:Gene Expression; D005814:Genes, Viral; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D034421:Sus scrofa / Q000276:immunology* / Q000821:virology; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D016056:Immunodominant Epitopes; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins