title: Catalyser-21(TM), a mineral water derived from leaf soil, inhibits tumor cell invasion and angiogenesis.
abstract: Catalyser-21(TM) is a mineral water derived from natural leaf soil containing various organic and inorganic substances. Previous reports suggested a possibility that Catalyser-21(TM) has antioxidative potential and could inhibit angiogenesis and cancer cell invasiveness. Angiogenesis is a prerequisite for cancer cells to spread to surrounding tissues. Vascular endothelial growth factor (VEGF) is a major angiogenic factor in the formation of blood capillaries by cancer cells to supply nutrients and oxygen for their sustained growth. Matrix metalloproteinase-2 (MMP-2) is another key enzyme for cancer cell metastasis. To assess the anti-angiogenic activity of Catalyser-21(TM), we first examined cell viability using a human cervical cancer cell line, HeLa, and a fibrosarcoma cell line, HT1080. The results showed that Catalyser-21(TM) decreased the viability of both cell types in a dose-dependent manner. Flow cytometric analysis proved that Catalyser-21(TM) scavenges intracellular H(2)O(2) in both cell types. RT-PCR demonstrated that both VEGF and MMP-2 gene transcription was suppressed after Catalyser-21(TM) treatment. Both Matrigel and tubule formation experiments showed an effect of Catalyser-21(TM). These results suggest that Catalyser-21(TM) has potential as an anti-tumor agent.
authors.affiliation: Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ye
authors.forename: Yuping
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authors.lastname: Li
authors.forename: Takeki
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authors.lastname: Hamasaki
authors.forename: Noboru
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authors.lastname: Nakamichi
authors.forename: Takeshi
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authors.lastname: Kawahara
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authors.lastname: Osada
authors.forename: Kiichiro
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authors.lastname: Teruya
authors.forename: Yuko
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authors.lastname: Kato
authors.forename: Kazuko
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authors.lastname: Toh
authors.forename: Masumi
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authors.lastname: Abe
authors.forename: Yoshinori
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authors.lastname: Katakura
authors.forename: Katsumi
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authors.lastname: Noguchi
authors.forename: Sanetaka
authors.initials: S
authors.lastname: Shirahata