[Transfecting keratinocyte and enhancing cell proliferation by integrated HPV11 DNA].
We constructed the plasmid containing the integrated open reading frame (ORF) HPV11 genome, and identified its function, to lay foundation for production of transgenic animal models of HPV11. Recombinant plasmid pQE-Trisystem- EGFP/HPV11 (pE/H) and pQE-Trisystem-EGFP/1.1 copy HPV11 (pE/1.1H) were constructed. Then, the human keratinocyte (KC) was transfected by pE/1.1H, pE/H and closed circular HPV11 and detected. The recombinant plasmid was successfully constructed. The expression of HPV11E6 gene was detected in the experimental group. Fluorescence was observed in the pE/1.1H and pE/H group. The HPV11, pE/H, and pE/1.1 enhanced the KC proliferation, with remarkable differences (P < 0.01) from the control group. Amongst the three experimental groups, pE/1.1H was found to be the weakest. The plasmid containing the integrated ORF of HPV11 (1.1 copy HPV11) genome was successfully constructed. The pE/1.1H had the same phenotype of wild-type HPV11 genome. It provided experimental material and methodological guidance for studying the low-risk HPV, as well as for the production of HPV11 transgenic mice.
Zhang
Yi
Y
Institute of Surgery and Daping Hospital, The Third Military Medical University, Chongqing 400042, China.
Lü
Fenglin
F
Chen
Caiyu
C
D049109:Cell Proliferation; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics; D052140:Human papillomavirus 11 / Q000235:genetics*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D014162:Transfection
D004279:DNA, Viral