Detection of macrophages in atherosclerotic tissue using magnetic nanoparticles and differential phase optical coherence tomography.
We demonstrate the detection of iron oxide nanoparticles taken up by macrophages in atherosclerotic plaque with differential phase optical coherence tomography (DP-OCT). Magneto mechanical detection of nanoparticles is demonstrated in hyperlipidemic Watanabe and balloon-injured fat-fed New Zealand white rabbits injected with monocrystalline iron oxide nanoparticles (MIONs) of < 40 nm diam. MIONs taken up by macrophages was excited by an oscillating magnetic flux density and resulting nanometer tissue surface displacement was detected by DP-OCT. Frequency response of tissue surface displacement in response to an externally applied magnetic flux density was twice the stimulus frequency as expected from the equations of motion for the nanoparticle cluster.
Oh
Junghwan
J
University of Texas at Austin, Biomedical Engineering Department, Austin, Texas 78712, USA.
Feldman
Marc D
MD
Kim
Jihoon
J
Sanghi
Pramod
P
Do
Dat
D
Mancuso
J Jacob
JJ
Kemp
Nate
N
Cilingiroglu
Mehmet
M
Milner
Thomas E
TE
D000818:Animals; D050197:Atherosclerosis / Q000473:pathology*; D003287:Contrast Media; D005290:Ferric Compounds; D007089:Image Enhancement / Q000379:methods; D008264:Macrophages / Q000473:pathology*; D008280:Magnetics; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D053758:Nanoparticles; D011817:Rabbits; D041623:Tomography, Optical Coherence / Q000379:methods*
D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide