Modified cetyltrimethylammonium bromide method improves robustness and versatility: the benchmark for plant RNA extraction.
A wide range of plant RNA extraction methods are available; however, many of these are limited in their application for a diverse range of plant species. With special emphasis on robustness and versatility, we have improved the cetyltrimethylammonium bromide (CTAB) method and isolated high-quality RNA from 16 different plant species. The major modifications made to the protocol described here were a reduction of sample treatment steps and an increase in beta-mercaptoethanol concentration (to 3%) resulting in a robust, rapid and reproducible plant RNA extraction protocol that can be used for a broad range of plant species and tissue types.
White
Elizabeth J
EJ
Department of Genetics, Stellenbosch University, Matieland, South Africa.
Venter
Mauritz
M
Hiten
Nicholas F
NF
Burger
Johan T
JT
D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D008623:Mercaptoethanol; D018515:Plant Leaves / Q000737:chemistry*; D018749:RNA, Plant / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D002593:Cetrimonium Compounds; D018749:RNA, Plant; D008623:Mercaptoethanol; D000077286:Cetrimonium