title: Silencing and transcriptional properties of the imprinted Airn ncRNA are independent of the endogenous promoter.
abstract: The Airn macro ncRNA is the master regulator of imprinted expression in the Igf2r imprinted gene cluster where it silences three flanking genes in cis. Airn transcription shows unusual features normally viewed as promoter specific, such as impaired post-transcriptional processing and a macro size. The Airn transcript is 108 kb long, predominantly unspliced and nuclear localized, with only a minority being variably spliced and exported. Here, we show by deletion of the Airn ncRNA promoter and replacement with a constitutive strong or weak promoter that splicing suppression and termination, as well as silencing activity, are maintained by strong Airn expression from an exogenous promoter. This indicates that all functional regions are located within the Airn transcript. DNA methylation of the maternal imprint control element (ICE) restricts Airn expression to the paternal allele and we also show that a strong active promoter is required to maintain the unmethylated state of the paternal ICE. Thus, Airn expression not only induces silencing of flanking mRNA genes but also protects the paternal copy of the ICE from de novo methylation.
authors.affiliation: CeMM, Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
authors.forename: Stefan H
authors.initials: SH
authors.lastname: Stricker
authors.forename: Laura
authors.initials: L
authors.lastname: Steenpass
authors.forename: Florian M
authors.initials: FM
authors.lastname: Pauler
authors.forename: Federica
authors.initials: F
authors.lastname: Santoro
authors.forename: Paulina A
authors.initials: PA
authors.lastname: Latos
authors.forename: Ru
authors.initials: R
authors.lastname: Huang
authors.forename: Martha V
authors.initials: MV
authors.lastname: Koerner
authors.forename: Mathew A
authors.initials: MA
authors.lastname: Sloane
authors.forename: Katarzyna E
authors.initials: KE
authors.lastname: Warczok
authors.forename: Denise P
authors.initials: DP
authors.lastname: Barlow
mesh_terms: D002478:Cells, Cultured; D019175:DNA Methylation; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D022661:RNA, Untranslated / Q000235:genetics*; D017384:Sequence Deletion; D014158:Transcription, Genetic
chemical_list: D022661:RNA, Untranslated