Identification of crude drugs from Chinese medicinal plants of the genus Bupleurum using ribosomal DNA ITS sequences.
To determine the variation of rDNA ITS sequences in medicinal plants of the genus Bupleurum and identify corresponding DNA molecular markers associated with medicinally important members, ITS regions were amplified, sequenced, and analyzed by DNAssist version 2.2. We found that the homologous alignment of ITS sequences between members of the genus Bupleurum and associated out-groups was lower than 75%, while within-group alignment was greater than 87%. The conclusion can be drawn that ITS sequences can be used as reliable molecular markers for the identification of Radix Bupleuri.
Xie
Hui
H
School of Pharmacy, Fudan University, Shanghai, PR China.
Huo
Ke-ke
KK
Chao
Zhi
Z
Pan
Sheng-li
SL
D029972:Bupleurum / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D005819:Genetic Markers; D010946:Plants, Medicinal / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity
D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers