Human adenovirus type 19: genomic and bioinformatics analysis of a keratoconjunctivitis isolate.
Human adenovirus type 19 (HAdV-19) is a major etiologic agent of epidemic keratoconjunctivitis (EKC), a common and severe eye infection associated with long-term visual morbidity due to persistent corneal inflammation. Ironically, while the prototype strain of HAdV-19 does not cause eye infections, other isolates of the serotype have caused major outbreaks of EKC. Here we have sequenced a clinical isolate of HAdV-19 (HAdV-19 strain C) from a human patient with EKC. Global pairwise alignment of HAdV-19C to other HAdV species D serotypes identified areas of sequence divergence in the penton base (host cell internalization signal), hexon (principal viral capsid structural protein), E3 (site of immunomodulatory genes), and fiber (host cell-binding ligand) regions. Comparison of HAdV-19 strain C to the recently sequenced HAdV-37, another EKC causing serotype, identified sequence diversity in the penton base and hexon, but sequence conservation in the E3 and fiber regions. Elucidation of the HAdV-19C genome will facilitate future studies into the pathogenesis of EKC, and may shed light on the genetic determinants of corneal tropism.
Robinson
Christopher M
CM
The Molecular Pathogenesis of Eye Infection Research Center, Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
Shariati
Fatemeh
F
Zaitshik
Jeremy
J
Gillaspy
Allison F
AF
Dyer
David W
DW
Chodosh
James
J
D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019295:Computational Biology; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D006801:Humans; D007637:Keratoconjunctivitis / Q000235:genetics / Q000821:virology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004279:DNA, Viral