Effect of carbohydrates on the ability of bull sperm to bind to bovine oviduct epithelial cells.
In the present study, we investigated the effect of various carbohydrates on the ability of bovine spermatozoa to bind to the bovine oviduct epithelial cells (OECs). We also examined the fertilization competence and motility of spermatozoa that bind to OECs in the presence of carbohydrates. Frozen-thawed spermatozoa were incubated with OECs, with and without various carbohydrates. The sperms were then divided into two fractions: OEC-binding sperms (B-sperm) and non-OEC binding sperms (NB-sperm). The fertilization rate, ability to bind the zona pellucida, and membrane integrity of the spermatozoa as determined using a hypo-osmotic-swelling test (HOST) were lower in NB-sperm than in the unseparated spermatozoa (control). The motility of the B-sperm was maintained for a longer time than that of the control spermatozoa. The addition of N-acetyl-d-glucosamine (GlcNAc, 5 mm) to the sperm-OEC mixture increased the number of B-sperm. D-mannose (5 mm) and D-fucose (5 mm) had no effect on the number of B-sperm. The motility of B-sperm, which bound to OECs in the presence of GlcNAc, however, was not maintained. When either OECs or the spermatozoa were treated with GlcNAc prior to sperm-OEC co-incubation, only sperm-side treatment enhanced sperm-OEC binding, but B-sperm motility was not maintained. The motility of spermatozoa incubated with GlcNAc was lower than that of controls. These results indicate that GlcNAc enhances sperm binding to OECs, probably via sperm surface modification, but does not promote increased sperm survival.
Kon
Y
Y
Department of Animal Science, Tokyo University of Agriculture, Atsugi, Japan.
Iwata
H
H
Shiono
H
H
Matsubara
K
K
Kurita
A
A
Sakaguchi
Y
Y
Kuwayama
T
T
Monji
Y
Y
D000117:Acetylglucosamine / Q000494:pharmacology; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D002417:Cattle / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000662:veterinary; D004847:Epithelial Cells / Q000378:metabolism*; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D005306:Fertilization / Q000187:drug effects; D005643:Fucose / Q000494:pharmacology; D006358:Hot Temperature; D008297:Male; D008358:Mannose / Q000494:pharmacology; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D015044:Zona Pellucida / Q000378:metabolism
D002241:Carbohydrates; D005643:Fucose; D008358:Mannose; D000117:Acetylglucosamine