title: Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation.
abstract: To understand the molecular mechanism underlying vigorous proliferative activity of hepatic stem-like (HSL) cells, we performed two-dimensional electrophoresis to identify the proteins statistically more abundant in rapidly growing undifferentiated HSL cells than in sodium butyrate-treated differentiated HSL cells. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry and Mascot search identified 6 proteins including prohibitin, vimentin, ezrin, annexin A3, acidic ribosomal phosphoprotein P0 and Grp75. Prohibitin and vimentin control the mitogen-activated protein (MAP) kinase pathway. Ezrin is phosphorylated by various protein-tyrosine kinases and modulates interactions between cytoskeletal and membrane proteins. Annexin A3 has a role in DNA synthesis. Acidic ribosomal phosphoprotein P0 and Grp75 play in protein synthesis. These results suggest that the proteins related to the MAP kinase cascade had some role in continuous proliferation of HSL cells without differentiation.
authors.affiliation: Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Kiryu, Gunma, 376-8515, Japan, saheki@chem-bio.gunma-u.ac.jp.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Saheki
authors.forename: Hitomi
authors.initials: H
authors.lastname: Ito
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sekiguchi
authors.forename: Atsuyoshi
authors.initials: A
authors.lastname: Nishina
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Izumi
authors.forename: Itaru
authors.initials: I
authors.lastname: Kojima