title: Both telomeric and non-telomeric DNA damage are determinants of mammalian cellular senescence.
abstract: BACKGROUND
  Cellular senescence is a state reached by normal mammalian cells after a finite number of cell divisions and is characterized by morphological and physiological changes including terminal cell-cycle arrest. The limits on cell division imposed by senescence may play an important role in both organismal aging and in preventing tumorigenesis. Cellular senescence and organismal aging are both accompanied by increased DNA damage, seen as the formation of gamma-H2AX foci (gamma-foci), which may be found on uncapped telomeres or at non-telomeric sites of DNA damage. However, the relative importance of telomere- and non-telomere-associated DNA damage to inducing senescence has never been demonstrated. Here we present a new approach to determine accurately the chromosomal location of gamma-foci and quantify the number of telomeric versus non-telomeric gamma-foci associated with senescence in both human and mouse cells. This approach enables researchers to obtain accurate values and to avoid various possible misestimates inherent in earlier methods.
  
  
  RESULTS
  Using combined immunofluorescence and telomere fluorescence in situ hybridization on metaphase chromosomes, we show that human cellular senescence is not solely determined by telomeric DNA damage. In addition, mouse cellular senescence is not solely determined by non-telomeric DNA damage. By comparing cells from different generations of telomerase-null mice with human cells, we show that cells from late generation telomerase-null mice, which have substantially short telomeres, contain mostly telomeric gamma-foci. Most notably, we report that, as human and mouse cells approach senescence, all cells exhibit similar numbers of total gamma-foci per cell, irrespective of chromosomal locations.
  
  
  CONCLUSIONS
  Our results suggest that the chromosome location of senescence-related gamma-foci is determined by the telomere length rather than species differences per se. In addition, our data indicate that both telomeric and non-telomeric DNA damage responses play equivalent roles in signaling the initiation of cellular senescence and organismal aging. These data have important implications in the study of mechanisms to induce or delay cellular senescence in different species.
authors.affiliation: Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, Rockville Pike, Bethesda, MD 20892, USA. nakamuraa@mail.nih.gov
authors.forename: Asako J
authors.initials: AJ
authors.lastname: Nakamura
authors.forename: Y Jeffrey
authors.initials: YJ
authors.lastname: Chiang
authors.forename: Karen S
authors.initials: KS
authors.lastname: Hathcock
authors.forename: Izumi
authors.initials: I
authors.lastname: Horikawa
authors.forename: Olga A
authors.initials: OA
authors.lastname: Sedelnikova
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Hodes
authors.forename: William M
authors.initials: WM
authors.lastname: Bonner