title: Aseptic technique.
abstract: This chapter describes common laboratory procedures that can reduce the risk of culture contaminations (sepsis), collectively referred as "aseptic technique." Two major strategies of aseptic work are described: using a Bunsen burner and a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri dishes.
authors.affiliation: Center for Medical Education, Warsaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bykowski
authors.forename: Brian
authors.initials: B
authors.lastname: Stevenson
mesh_terms: D001207:Asepsis / Q000379:methods*; D019411:Clinical Laboratory Techniques