A novel L-amino acid ligase is encoded by a gene in the phaseolotoxin biosynthetic gene cluster from Pseudomonas syringae pv. phaseolicola 1448A.
In the phaseolotoxin biosynthetic gene cluster of Pseudomonas syringae pv. phaseolicola 1448A, the PSPPH_4299 gene encodes a novel L-amino acid ligase. The PSPPH_4299 protein synthesized various hetero-dipeptides containing basic amino acids in an ATP-dependent manner, and also synthesized alanyl-homoarginine, part of the phaseolotoxin scaffold.
Arai
Toshinobu
T
Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, Tokyo, Japan.
Kino
Kuniki
K
D004151:Dipeptides / Q000096:biosynthesis; D005798:Genes, Bacterial / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics*; D009952:Ornithine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D010453:Peptide Synthases / Q000235:genetics* / Q000378:metabolism; D044224:Pseudomonas syringae / Q000201:enzymology* / Q000235:genetics*; D013379:Substrate Specificity
D004151:Dipeptides; C021234:phaseolotoxin; D009952:Ornithine; D010453:Peptide Synthases