Antibody-labeled fluorescence imaging of dendritic cell populations in vivo.
We report an optical molecular imaging technique that exploits local administration of fluorophore-conjugated antibodies and confocal fluorescence microscopy to achieve high-contrast imaging of host cell populations in normal and tumor tissue in living mice. The method achieves micron-scale spatial resolution to depths greater than 100 mum. We illustrate the capabilities of this approach by imaging two dendritic cell populations in the skin and normal and tumor vasculature in vivo.
Cummings
Ryan J
RJ
University of Rochester Medical Center ,Department of Microbiology and Immunology, Rochester, New York 14642, USA.
Mitra
Soumya
S
Lord
Edith M
EM
Foster
Thomas H
TH
D000818:Animals; D000911:Antibodies, Monoclonal; D003713:Dendritic Cells / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence / Q000379:methods*; D012867:Skin / Q000166:cytology*; D012878:Skin Neoplasms / Q000473:pathology*
D000911:Antibodies, Monoclonal