title: Development of porous collagen beads for chondrocyte culture.
abstract: A method for the preparation of bioresorbable collagen beads with an open porous structure is presented. These beads were prepared from collagen-alginate composite beads by removal of the alginate component. These collagen beads were suitable for rapid proliferation of chondrocytes in a dynamic, spinner culture system. Histology and immuno-histology showed that biochemical markers of chondrocytes are present during this cell proliferation, indicating that there was control of phenotype and that cell de-differentiation had not occurred. Unlike other 3-D scaffolds that have been used, the cells were amplified from very low cell densities and were able to proliferate freely without loss of phenotype.
authors.affiliation: CSIRO, Molecular and Health Technologies, Bayview Avenue, Clayton South, VIC, 3169, Australia.
authors.forename: Tracy A
authors.initials: TA
authors.lastname: Tebb
authors.forename: Shiao-Wen
authors.initials: SW
authors.lastname: Tsai
authors.forename: Veronica
authors.initials: V
authors.lastname: Glattauer
authors.forename: Jacinta F
authors.initials: JF
authors.lastname: White
authors.forename: John A M
authors.initials: JA
authors.lastname: Ramshaw
authors.forename: Jerome A
authors.initials: JA
authors.lastname: Werkmeister