Fluctuations of intracellular forces during cell protrusion.
We present a model to estimate intracellular force variations from live-cell images of actin filament (F-actin) flow during protrusion-retraction cycles of epithelial cells in a wound healing response. To establish a mechanistic relationship between force development and cytoskelal dynamics, force fluctuations were correlated with fluctuations in F-actin turnover, flow and F-actin-vinculin coupling. Our analyses suggest that force transmission at focal adhesions requires binding of vinculin to F-actin and integrin (indirectly), which is modulated at the vinculin-integrin but not the vinculin-F-actin interface. Force transmission at focal adhesions is colocalized in space and synchronized in time with transient increases in the boundary force at the cell edge. Surprisingly, the maxima in adhesion and boundary forces lag behind maximal edge advancement by about 40 s. Maximal F-actin assembly was observed about 20 s after maximal edge advancement. On the basis of these findings, we propose that protrusion events are limited by membrane tension and that the characteristic duration of a protrusion cycle is determined by the efficiency in reinforcing F-actin assembly and adhesion formation as tension increases.
Ji
Lin
L
Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Lim
James
J
Danuser
Gaudenz
G
D000199:Actins / Q000378:metabolism; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002465:Cell Movement; D048430:Cell Shape; D003599:Cytoskeleton / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism*; D008954:Models, Biological; D011485:Protein Binding; D011554:Pseudopodia / Q000378:metabolism*; D013997:Time Factors; D016596:Vinculin / Q000378:metabolism
D000199:Actins; D016023:Integrins; D016596:Vinculin