High density and scale-up cultivation of recombinant CHO cell line and hybridomas with porous microcarrier Cytopore.
Using porous microcarrier Cytopore and a low-serum medium supplement BIGBEF-3, we have successfully cultivated recombinant CHO cell line CL-11G producing prourokinase and hybridomas producing anti-prourokinase monoclonal antibody in Celligen 1.5 or 5 L bioreactor. The cell density obtained ranged from 1 to 2 x 107 cells mL-1. The yields of prourokinase and monoclonal antibody increased with increasing cell density. As the cells could spontaneously release from and reattach to porous microcarriers, it was very easy to scale-up the cultivation. Thus the bead to bead cell transfer method has been used to scale up the cultivation of CL-11G cells to a 20 L reactor-scale for the pilot production of prourokinase, and also to scale-up the culture of hybridomas for the production of monoclonal antibody for the purification of prourokinase.
Xiao
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Department of Cell Engineering, Institute of Biotechnology, 20 Dongdajie, Fengtai, Beijing, 100071, P.R. China.
Huang
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Li
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Hu
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Qu
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Gao
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L
Liu
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