title: Monocyte-derived microparticles and exosomes induce procoagulant and apoptotic effects on endothelial cells.
abstract: Microvesicles (MVs) which include microparticles (MPs) and exosomes are found in blood circulation in normal physiologic conditions and are increased in a variety of diseases. This study evaluated the effects of MVs on human umbilical vein endothelial cells (HUVEC) by morphologic changes, apoptosis, and thrombogenicty, in vitro. Stimulation of monocyte cell line (THP-1) by starvation or by endotoxin and calcium ionophore A23187 resulted in the release of MVs which express exosome marker Tsg 101, negative phospholipids in their leaflets, monocyte markers (CD18, CD14) and active tissue factor (TF). MVs were found to disrupt EC integrity and rapidly induce membrane blebbing. Brief exposure (2-4 hours) to MVs resulted in EC membrane phospholipids "flip-flop" while longer stimulation (20 hours) led to two contradicting outcomes - tube formation as well as apoptosis, as assessed by nuclear fragmentation. Additionally, MVs exposure resulted in increased cell surface thrombogenicity and perturbation of the endothelial haemostatic balance, which were enhanced during longer exposure time. Activity, antigen level and mRNA expression of the coagulation initiator TF were elevated due to (i) adherence of MVs derived TF to the EC membrane, and (ii) an increase in endothelial TF expression. Furthermore, levels of the anticoagulant tissue factor pathway inhibitor (TFPI) and thrombomodulin (TM) were decreased. These findings demonstrate that monocyte MVs increase endothelial thrombogenicity and apoptosis. In addition, they induce tube formation which may indicate their angiogenic effect. These findings may clarify, in part, the role of MVs in EC dysfunction associated with inflammatory diseases and hypercoagulable states.
authors.affiliation: Rambam Health Care Campus, Department of Hematology and Bone marrow Transplantation, Bat Galim, Haifa, 31096, Israel. a_aharon@yahoo.com
authors.forename: Anat
authors.initials: A
authors.lastname: Aharon
authors.forename: Tal
authors.initials: T
authors.lastname: Tamari
authors.forename: Benjamin
authors.initials: B
authors.lastname: Brenner
mesh_terms: D017209:Apoptosis; D001777:Blood Coagulation / Q000235:genetics; D002454:Cell Differentiation; D048430:Cell Shape; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D055354:Exosomes / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D018919:Neovascularization, Physiologic; D012333:RNA, Messenger / Q000378:metabolism; D018180:Thrombomodulin / Q000378:metabolism; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D008074:Lipoproteins; D012333:RNA, Messenger; D018180:Thrombomodulin; C051928:lipoprotein-associated coagulation inhibitor; D013925:Thromboplastin