Cerebrolysin and morphine decrease glutathione and 5-hydroxyindole acetic acid levels in fasted rat brain.
OBJECTIVE
The aim was to evaluate if morphine sulphate combined with cerebrolysin enhances the risk of oxidative damage in the presence of moderate hypoglycaemia.


METHODS
Wistar rats under starvation for 48h received a single dose of 215 mg/kg cerebrolysin or 4 mg/kg morphine sulphate. Glutathione (GSH) and 5-hydroxyindoleacetic acid (5-HIAA) levels were measured in brain tissue, as well as lipid peroxidation, Na(+)-K(+) ATPase and total ATPase enzymatic activities, by fluorescence and spectrophotometric methods.


RESULTS
GSH and 5-HIAA levels decreased significantly (p<0.05) in animals which received cerebrolysin and morphine alone or combined. TBARS levels increased in all groups, but the values were statistically significant only in those animals that received cerebrolysin combined with morphine (p<0.05). Na(+)-K(+) ATPase and total ATPase activities decreased significantly in rats treated only with morphine, but the cerebrolysin and morphine groups showed a significant increase in these enzymatic activities.


CONCLUSIONS
Results suggest that cerebrolysin as well as morphine induced changes in cellular regulation and biochemical responses to oxidative stress induced by moderate hypoglycaemia in brain.
Guzmán
David Calderón
DC
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), CP 04530, Mexico City, Mexico.
Brizuela
Norma Osnaya
NO
Alvarez
Raquel García
RG
García
Ernestina Hernández
EH
Mejía
Gerardo Barragán
GB
Olguín
Hugo Juárez
HJ
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004347:Drug Interactions; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism*; D007003:Hypoglycemia / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D009020:Morphine / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000596:Amino Acids; D000975:Antioxidants; D018696:Neuroprotective Agents; D017392:Thiobarbituric Acid Reactive Substances; C006952:cerebrolysin; D006897:Hydroxyindoleacetic Acid; D009020:Morphine; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione