Endothelial Src kinase regulates membrane recycling from the lateral border recycling compartment during leukocyte transendothelial migration.
When leukocytes cross endothelial cells during the inflammatory response, membrane from the recently described lateral border recycling compartment (LBRC) is selectively targeted around diapedesing leukocytes. This "targeted recycling" is critical for leukocyte transendothelial migration. Blocking homophilic PECAM interactions between leukocytes and endothelial cells blocks targeted recycling from the LBRC and blocks diapedesis. However, the cellular signaling pathways that trigger targeted recycling are not known. We show that targeted recycling from the LBRC is dependent on Src kinase. The selective Src kinase inhibitor PP2 blocked targeted recycling and blocked diapedesis by over 70%. However, Src kinase inhibition did not affect the structure or normal constitutive recycling of membrane from the LBRC in the absence of leukocytes. PECAM, a Src kinase substrate, traffics between the LBRC and the endothelial surface at the cell border. However, virtually all of the PECAM in the cell that was phosphorylated on tyrosine residues was found in the LBRC. These findings demonstrate that Src kinase activity is critical for the targeted recycling of membrane from the LBRC to the site of transendothelial migration and that the PECAM in the LBRC is qualitatively different from the PECAM on the surface of endothelial cells.
Dasgupta
Bidisha
B
Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY, USA.
Muller
William A
WA
D015815:Cell Adhesion Molecules / Q000378:metabolism; D002465:Cell Movement / Q000276:immunology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D006801:Humans; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D019000:Phosphotyrosine / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D014470:Umbilical Cord / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D047428:Protein Kinase Inhibitors; D019000:Phosphotyrosine; D019061:src-Family Kinases