Utilization of fermented barley extract obtained from a by-product of barley shochu for nisin production.
Fermented barley extract (FBE) obtained from a barley shochu by-product (shochu kasu) and its ethanol fractions were evaluated as a medium and supplement, respectively, for nisin A production by Lactococcus lactis subsp. lactis ATCC 11454. A Brix 2.5 FBE medium supplemented with glucose provided a high level of nisin A production with a nisin yield comparable to that from a nutritionally rich laboratory medium (basal medium). By adding the ethanol-insoluble (EI) fraction of FBE to the basal medium, nisin A production was enhanced concomitant with an increase in bacterial cell growth, while the ethanol-soluble (ES) fraction had a negative effect on nisin A production. These findings indicate that FBE obtained from shochu kasu can be utilized as a preferable medium for nisin A production and could be converted into a value-added food product having preservative functions. The procedure developed in this study would promote recycling of shochu kasu.
Furuta
Yoshifumi
Y
Research Laboratory, Sanwa Shurui, Co. Ltd., 2231-1 Yamamoto, Usa, Oita 879-0495, Japan. furuta-y@kokuzo.co.jp
Maruoka
Naruyuki
N
Nakamura
Akihiro
A
Omori
Toshiro
T
Sonomoto
Kenji
K
D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D001467:Hordeum / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D013294:Lactococcus lactis / Q000378:metabolism; D009561:Nisin / Q000096:biosynthesis* / Q000737:chemistry; D012995:Solubility; D013696:Temperature
D003470:Culture Media; D007773:Lactates; D009561:Nisin; D019344:Lactic Acid; D000431:Ethanol; C102526:nisin A; D005947:Glucose