title: Serotonin receptor 2A blocker (risperidone) has no effect on human polyomavirus JC infection of primary human fetal glial cells.
abstract: A recent report demonstrated that JC virus (JCV) employs serotonin receptor 2A (5HT(2A)R) to infect the glial cells. To assess the ability of a potent 5HT(2A)R blocker, risperidone, to inhibit JCV infection, the authors treated primary human fetal glial (PHFG) cells in vitro with risperidone for 24 h and inoculated with JCV(Mad1). There was no significant difference in JCV genome copies or mRNA transcripts and protein expression in treatment-naive and risperidone-treated PHFG cells. These data indicate that risperidone does not inhibit JCV(Mad1) attachment, internalisation, and replication in PHFG cells, and 5HT(2A)R blockers may not be effective in treating progressive multifocal leukoencephalopathy (PML).
authors.affiliation: Retrovirology Research Laboratory, Department of Tropical Medicine, Medical Microbiology and Pharmacology, Asia-Pacific Institute of Tropical Medicine and Infectious Diseases, John A. Burns School of Medicine, Honolulu, Hawaii 96813, USA.
authors.forename: Moti L
authors.initials: ML
authors.lastname: Chapagain
authors.forename: Laarni
authors.initials: L
authors.lastname: Sumibcay
authors.forename: Ulziijargal
authors.initials: U
authors.lastname: Gurjav
authors.forename: Pakieli H
authors.initials: PH
authors.lastname: Kaufusi
authors.forename: Richard E
authors.initials: RE
authors.lastname: Kast
authors.forename: Vivek R
authors.initials: VR
authors.lastname: Nerurkar
mesh_terms: D002460:Cell Line; D005333:Fetus / Q000821:virology; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D006801:Humans; D007577:JC Virus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D009457:Neuroglia / Q000821:virology*; D027601:Polyomavirus Infections / Q000235:genetics / Q000821:virology*; D044402:Receptor, Serotonin, 5-HT2A / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018967:Risperidone / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D044402:Receptor, Serotonin, 5-HT2A; D018967:Risperidone