title: Analysis of protein lysine acetylation in vitro and in vivo.
abstract: Protein lysine acetylation, referring to acetylation of the epsilon-amino group of a lysine residue, has recently emerged as an important post-translational modification for regulating protein functions in various organisms. Like phosphorylation, lysine acetylation is a rapidly reversible and precisely controlled covalent modification that serves as a simple on/off switch or participates in a codified manner with other post-translational modifications to regulate protein functions in different cellular and developmental processes. This unit describes and discusses methods used for in vitro and in vivo determination of lysine acetylation.
authors.affiliation: Rosalind and Morris Goodman Cancer Center and Department of Medicine, McGill University, Montreal, Canada.
authors.forename: Nadine
authors.initials: N
authors.lastname: Pelletier
authors.affiliation: Rosalind and Morris Goodman Cancer Center and Department of Medicine, McGill University, Montreal, Canada.
authors.forename: Serge
authors.initials: S
authors.lastname: Grégoire
authors.affiliation: Rosalind and Morris Goodman Cancer Center and Department of Medicine, McGill University, Montreal, Canada.
authors.forename: Xiang-Jiao
authors.initials: XJ
authors.lastname: Yang
mesh_terms: D000107:Acetylation; D001345:Autoradiography; D001671:Biochemistry / Q000379:methods*; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D006801:Humans; D008239:Lysine / Q000032:analysis*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis*; D011851:Radioactivity
chemical_list: D011506:Proteins; D002482:Cellulose; C008881:phosphocellulose; D008239:Lysine