title: Neonatal exposure to genistein disrupts ability of female mouse reproductive tract to support preimplantation embryo development and implantation.
abstract: Female mice treated neonatally with the phytoestrogen genistein (50 mg/kg/day) have multioocyte follicles, lack regular estrous cyclicity, and are infertile even after superovulation. To determine the cause of their infertility, we examined oocyte developmental competence and timing of embryo loss. Eggs obtained by superovulation of genistein-treated or control females were equally capable of being fertilized in vitro and cultured to the blastocyst stage. However, if eggs were fertilized in vivo, retrieved at the pronucleus stage, and cultured, there was a significant reduction in the percentage of embryos from genistein-treated females reaching the blastocyst stage. When these blastocysts were transferred to pseudopregnant recipients, the number of live pups produced was similar to that in controls. Preimplantation embryo development in vivo was examined by flushing embryos from the oviduct and/or uterus. Similar numbers of one-cell and two-cell embryos were obtained from genistein-treated and control females. However, significantly fewer embryos (<50%) were obtained from genistein-treated females on postcoital Days 3 and 4. To determine if neonatal genistein treatment altered the ability of the uterus to support implantation, blastocysts from control donors were transferred to control and genistein-treated pseudopregnant recipients. These experiments demonstrated that genistein-treated females are not capable of supporting normal implantation of control embryos. Taken together, these results suggest that oocytes from mice treated neonatally with genistein are developmentally competent; however, the oviductal environment and the uterus have abnormalities that contribute to the observed reproductive failure.
authors.affiliation: Reproductive Medicine Group, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA.
authors.forename: Wendy N
authors.initials: WN
authors.lastname: Jefferson
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Padilla-Banks
authors.forename: Eugenia H
authors.initials: EH
authors.lastname: Goulding
authors.forename: Shin-Ping C
authors.initials: SP
authors.lastname: Lao
authors.forename: Retha R
authors.initials: RR
authors.lastname: Newbold
authors.forename: Carmen J
authors.initials: CJ
authors.lastname: Williams
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology; D004624:Embryo Transfer; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology; D019833:Genistein / Q000494:pharmacology / Q000633:toxicity*; D007247:Infertility, Female / Q000139:chemically induced* / Q000503:physiopathology; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010053:Ovary / Q000187:drug effects / Q000503:physiopathology; D010057:Oviducts / Q000187:drug effects / Q000503:physiopathology; D048789:Phytoestrogens / Q000494:pharmacology / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced* / Q000503:physiopathology; D014599:Uterus / Q000187:drug effects / Q000503:physiopathology
chemical_list: D048789:Phytoestrogens; D019833:Genistein