title: Stem-cell culture on patterned bio-functional surfaces.
abstract: Bio-functional surfaces have been created by printing proteins on antifouling surfaces in a customised geometry. Human umbilical cord neural stem cells incubated on the samples readily attach to the protein defined domains, where they have been monitored during 21 days of culture. The stability of the pattern varies with the density of cells anchored to the microstamped proteins. Highly packed cell patterned domains favoured non-differentiated mode, while low-density areas allowed the spreading out of the cells and differentiation. Tailoring the geometry (pattern size and distances) enables improving the monitoring of the stem cells' developmental processes. The biocompatible surfaces can serve as a model to study processes accompanying stem cell neural lineage commitment.
authors.affiliation: Institute for Health and Consumer Protection, Joint Research Centre, European Commission, TP 203, Via E Fermi, 21020 Ispra (VA), Italy.
authors.forename: A
authors.initials: A
authors.lastname: Ruiz
authors.forename: L
authors.initials: L
authors.lastname: Buzanska
authors.forename: L
authors.initials: L
authors.lastname: Ceriotti
authors.forename: F
authors.initials: F
authors.lastname: Bretagnol
authors.forename: S
authors.initials: S
authors.lastname: Coecke
authors.forename: P
authors.initials: P
authors.lastname: Colpo
authors.forename: F
authors.initials: F
authors.lastname: Rossi
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D011327:Printing; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013499:Surface Properties; D014404:Tubulin / Q000378:metabolism
chemical_list: D008869:Microtubule-Associated Proteins; D014404:Tubulin