Deuterated polymer gels for measuring anisotropic NMR parameters with strongly reduced artefacts.
Perdeuterated poly(styrene) is introduced as an almost artefact-free and arbitrarily scalable alignment medium for measuring residual dipolar couplings and other anisotropic NMR parameters; the spectral quality achievable in this new medium is demonstrated for HSQC spectra leading to the conformational analysis of staurosporine and homonuclear TOCSY-type experiments.
Kummerlöwe
Grit
G
Center of Integrated Protein Science at the Department of Chemistry, Technische Universität München, Lichtenbergstr. 4, 85747, Garching, Germany.
Knör
Sebastian
S
Frank
Andreas O
AO
Paululat
Thomas
T
Kessler
Horst
H
Luy
Burkhard
B
D016880:Anisotropy; D016477:Artifacts; D003903:Deuterium / Q000737:chemistry; D005782:Gels / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011137:Polystyrenes / Q000737:chemistry*; D019311:Staurosporine / Q000737:chemistry
D005782:Gels; D011137:Polystyrenes; D003903:Deuterium; D019311:Staurosporine