Cinnamon extract inhibits the postprandial overproduction of apolipoprotein B48-containing lipoproteins in fructose-fed animals.
We have reported previously that a cinnamon extract (CE), high in type A polyphenols, prevents fructose feeding-induced decreases in insulin sensitivity and suggested that improvements of insulin sensitivity by CE were attributable, in part, to enhanced insulin signaling. In this study, we examined the effects of CE on postprandial apolipoprotein (apo) B-48 increase in fructose-fed rats, and the secretion of apoB48 in freshly isolated intestinal enterocytes of fructose-fed hamsters. In an olive oil loading study, a water-soluble CE (Cinnulin PF, 50 mg/kg body weight, orally) decreased serum triglyceride (TG) levels and the over production of total- and TG-rich lipoprotein-apoB48. In ex vivo (35)S labeling study, significant decreases were also observed in apoB48 secretion into the media in enterocytes isolated from fructose-fed hamsters. We also investigated the molecular mechanisms of the effects of CE on the expression of genes of the insulin signaling pathway [insulin receptor (IR), IR substrate (IRS)1, IRS2 and Akt1], and lipoprotein metabolism [microsomal TG transfer protein (MTP), sterol regulatory element-binding protein (SREBP1c) in isolated primary enterocytes of fructose-fed hamsters, using quantitative real-time polymerase chain reaction. The CE reversed the expression of the impaired IR, IRS1, IRS2 and Akt1 mRNA levels and inhibited the overexpression of MTP and SREBP1c mRNA levels of enterocytes. Taken together, our data suggest that the postprandial hypertriglycerides and the overproduction of apoB48 can be acutely inhibited by a CE by a mechanism involving improvements of insulin sensitivity of intestinal enterocytes and regulation of MTP and SREBP1c levels. We present both in vivo and ex vivo evidence that a CE improves the postprandial overproduction of intestinal apoB48-containing lipoproteins by ameliorating intestinal insulin resistance and may be beneficial in the control of lipid metabolism.
Qin
Bolin
B
Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA. bolin.qin@ars.usda.gov
Polansky
Marilyn M
MM
Sato
Yuzo
Y
Adeli
Khosrow
K
Anderson
Richard A
RA
D000821:Animal Feed; D000818:Animals; D053283:Apolipoprotein B-48 / Q000096:biosynthesis*; D002352:Carrier Proteins / Q000096:biosynthesis; D002784:Cholesterol / Q000097:blood; D002935:Cinnamomum zeylanicum / Q000737:chemistry*; D006224:Cricetinae; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism; D005632:Fructose / Q000494:pharmacology*; D055504:Insulin Receptor Substrate Proteins / Q000096:biosynthesis; D007333:Insulin Resistance / Q000502:physiology; D008297:Male; D008647:Mesocricetus; D010936:Plant Extracts / Q000494:pharmacology*; D019518:Postprandial Period / Q000187:drug effects / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051780:Sterol Regulatory Element Binding Protein 1 / Q000096:biosynthesis; D014280:Triglycerides / Q000097:blood
D053283:Apolipoprotein B-48; D002352:Carrier Proteins; D004040:Dietary Carbohydrates; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D010936:Plant Extracts; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides; C546455:cinnulin PF; C106092:microsomal triglyceride transfer protein; D005632:Fructose; D002784:Cholesterol; C494920:Akt1 protein, rat; D051057:Proto-Oncogene Proteins c-akt