title: Novel Leb-like Helicobacter pylori-binding glycosphingolipid created by the expression of human alpha-1,3/4-fucosyltransferase in FVB/N mouse stomach.
abstract: The "Le(b) mouse" was established as a model for investigations of the molecular events following Le(b)-mediated adhesion of Helicobacter pylori to the gastric epithelium. By the expression of a human alpha-1,3/4-fucosyltransferase in the gastric pit cell lineage of FVB/N transgenic mice, a production of Le(b) glycoproteins in gastric pit and surface mucous cells was obtained in this "Le(b) mouse," as demonstrated by binding of monoclonal anti-Le(b) antibodies. To explore the effects of the human alpha-1,3/4-fucosyltransferase on glycosphingolipid structures, neutral glycosphingolipids were isolated from stomachs of transgenic alpha-1,3/4-fucosyltransferase-expressing mice. A glycosphingolipid recognized by BabA-expressing H. pylori was isolated and characterized by mass spectrometry and proton NMR as Fuc alpha 2Gal beta 3(Fuc alpha 4)GalNAc beta 4 Gal beta 4 Glc beta 1Cer, i.e., a novel Le(b)-like glycosphingolipid on a ganglio core. In addition, two other novel glycosphingolipids were isolated from the mouse stomach epithelium that were found to be nonbinding with regard to H. pylori. The first was a pentaglycosylceramide, GalNAc beta 3 Gal alpha 3(Fuc alpha 2)Gal beta 4 Glc beta 1Cer, in which the isoglobotetrasaccharide has been combined with Fuc alpha 2 to yield an isoglobotetraosylceramide with an internal blood group B determinant. The second one was an elongated fucosyl-gangliotetraosylceramide, GalNAc beta 3(Fuc alpha 2)Gal beta 3GalNAc beta 4Gal beta 4 Glc beta 1Cer.
authors.affiliation: Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, PO Box 440, University of Gothenburg, S-405 30 Göteborg, Sweden.
authors.forename: David
authors.initials: D
authors.lastname: Fagerberg
authors.forename: Jonas
authors.initials: J
authors.lastname: Angström
authors.forename: Adnan
authors.initials: A
authors.lastname: Halim
authors.forename: Anna
authors.initials: A
authors.lastname: Hultberg
authors.forename: Lena
authors.initials: L
authors.lastname: Rakhimova
authors.forename: Lennart
authors.initials: L
authors.lastname: Hammarström
authors.forename: Thomas
authors.initials: T
authors.lastname: Borén
authors.forename: Susann
authors.initials: S
authors.lastname: Teneberg
mesh_terms: D000818:Animals; D001665:Binding Sites; D005647:Fucosyltransferases / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006028:Glycosphingolipids / Q000737:chemistry* / Q000378:metabolism*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D008822:Mice, Transgenic; D013270:Stomach / Q000737:chemistry* / Q000201:enzymology
chemical_list: D006023:Glycoproteins; D006028:Glycosphingolipids; C024050:Le(b)-active glycolipid; D005647:Fucosyltransferases; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase