title: Characterization of epithelial-to-mesenchymal transition of mesothelial cells in a mouse model of chronic peritoneal exposure to high glucose dialysate.
abstract: Animal models of peritoneal dialysis fluid (PDF) exposure are key tools in the study of mechanisms involved in alterations of the peritoneal membrane and in the design of therapies. We recently developed a mouse model of chronic peritoneal exposure to high glucose dialysate. Herein, we make a sequential analysis of the effects of glucose-based PDF on mouse peritoneal membrane and on mesothelium. We demonstrate that chronic exposure to PDF induces thickness and fibrosis of the peritoneal membrane in a time-dependent manner. We also show that mesothelial cells progressively detach and lose cytokeratin expression. In addition, we demonstrate that some mesothelial cells invade the submesothelial space, where they appear as cytokeratin- and alpha-smooth muscle actin-positive cells. These findings demonstrate that epithelial-to-mesenchymal transition (EMT) of mesothelial cells takes place in mouse peritoneum exposed to PDF, validating this model for the study of effects of drugs on the EMT process as a therapy for peritoneal deterioration.
authors.affiliation: Unidad de Investigación and Servicio de Nefrología, Hospital Universitario La Paz.
authors.forename: Luiz S
authors.initials: LS
authors.lastname: Aroeira
authors.forename: Jesús
authors.initials: J
authors.lastname: Loureiro
authors.forename: Guadalupe T
authors.initials: GT
authors.lastname: González-Mateo
authors.forename: Vanessa
authors.initials: V
authors.lastname: Fernandez-Millara
authors.forename: Gloria
authors.initials: G
authors.lastname: del Peso
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Sánchez-Tomero
authors.forename: Marta
authors.initials: M
authors.lastname: Ruiz-Ortega
authors.forename: M Auxiliadora
authors.initials: MA
authors.lastname: Bajo
authors.forename: Manuel
authors.initials: M
authors.lastname: López-Cabrera
authors.forename: Rafael
authors.initials: R
authors.lastname: Selgas
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D015314:Dialysis Solutions / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000187:drug effects*; D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D008648:Mesoderm / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010530:Peritoneal Dialysis / Q000009:adverse effects*; D010537:Peritoneum / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
chemical_list: D015314:Dialysis Solutions; D005947:Glucose