title: High-throughput multiclass method for antibiotic residue analysis by liquid chromatography-tandem mass spectrometry.
abstract: A simple and rapid method has been developed for the residue analysis of 39 antibiotics (tetracyclines, quinolones, penicillins, sulfonamides and macrolides) in foodstuffs of animal origin. The method combines an effective extraction technique, which uses water-methanol as extracting solvent, with ultra-high-pressure liquid chromatography-tandem mass spectrometry, allowing both confirmation and quantification in a single chromatographic run. The multiresidue method has been validated in chicken muscle matrix according to European Union Decision 2002/657/EC. It has been implemented as a routine method in a Public Health Laboratory, instead of the five plates test and LC methods previously used.
authors.affiliation: Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Chico
authors.forename: A
authors.initials: A
authors.lastname: Rúbies
authors.forename: F
authors.initials: F
authors.lastname: Centrich
authors.forename: R
authors.initials: R
authors.lastname: Companyó
authors.forename: M D
authors.initials: MD
authors.lastname: Prat
authors.forename: M
authors.initials: M
authors.lastname: Granados
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D018942:Macrolides / Q000032:analysis; D008460:Meat / Q000032:analysis; D009132:Muscles / Q000737:chemistry; D010406:Penicillins / Q000032:analysis; D015363:Quinolones / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013449:Sulfonamides / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods; D013754:Tetracyclines / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D010406:Penicillins; D015363:Quinolones; D013449:Sulfonamides; D013754:Tetracyclines