[Screening on alkaloid of Sophora alopecuraides against adenocarcinoma of colon cell line SW620 in vitro].
OBJECTIVE
To test 5 kinds of alkaloid of Sophora alopecuraides L., the effects of TBSA, MT, OMT, SC, SRI against adenocarcinoma of colon cell line SW620.


METHODS
SW620 cell line was treated with alkaloid at various concentrations. The effect on cell proliferative activity was measured by MTT assay and the effects of alkaloids against cell growth were compared. The changes of cell morphology were observed through optical microscope and fluorescence microscope and the cell apoptosis was detected by flow cytometry and Annexin V-FITC apoptosis assay after the treatments.


RESULTS
According to the results of MTT assay, all of the 5 kinds of alkaloid especially SRI and MT were identified to produce growth inhibition against SW620 cells. The sequence of the intensity of effect was TBSA < OMT < SC < MT < SRI. Further study of SRI and MT demonstrated their effects on inducing SW620 cell apoptosis, and SRI showed the strongest effect. Both SRI and MT induced SW620 cells to apoptosis in a dose-and-time-dependent manner.


CONCLUSIONS
The 5 kinds of alkaloid especially SRI and MT may inhibit the growth of SW620 cells,and induce SW620 cell apoptosis. The results of this study pave the way for further identification of the effective components in Sophora alopecuraides L. that inhibit colorectal carcinoma both in vivo and in vitro.
Liang
Lei
L
School of TCM, Southern Medical University, Guangzhou 510515, China. libraliang78@126.com
Wang
Xiao-Yan
XY
Zhang
Xu-Hui
XH
Zhao
Wen-Chang
WC
Deng
Hong-Zhu
HZ
D000230:Adenocarcinoma / Q000473:pathology / Q000648:ultrastructure; D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D015179:Colorectal Neoplasms / Q000473:pathology / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006801:Humans; D011807:Quinolizines / Q000494:pharmacology; D029909:Sophora / Q000737:chemistry; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D011807:Quinolizines; D000093842:Matrines