[Influence of assisted reproduction materials on germ cells and embryos].
OBJECTIVE
To investigate the influence of the materials commonly used in the assisted reproduction procedure on human germ cells and embryo development.


METHODS
We used human sperm survival assay to detect the influence of two brands of culture dishes, two brands of injection needles, washed and unwashed ovum aspiration needles, embryo transfer catheters and surgical gloves on sperm motility. All the data obtained went through variance analysis with SPSS 13.0.


RESULTS
Sperm motility differed significantly between Nunclons and Falcon's culture dishes (52.68 +/-16.21 vs 45.36 +/- 15.25, P < 0.01) but not between the BD and the Jie-rui injection needles (P > 0.05), nor among the washed and unwashed ovum aspiration needles, embryo transfer catheters and surgical gloves (P > 0.05).


CONCLUSIONS
Different brands of assisted reproduction materials of similar use had different or similar influences on germ cells and embryos, while no difference existed in the influences of the washed and unwashed ovum aspiration needles, embryo transfer catheters and surgical gloves.
Hu
Bo
B
Department of Reproductive Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China.
Gong
Xiao-Yun
XY
Zhao
Jing
J
Zhu
Hong-Yan
HY
Cai
Xia
X
D017077:Culture Media, Conditioned; D047108:Embryonic Development; D006801:Humans; D008297:Male; D027724:Reproductive Techniques, Assisted / Q000295:instrumentation*; D013081:Sperm Motility
D017077:Culture Media, Conditioned