Triptolide inhibits amyloid-beta1-42-induced TNF-alpha and IL-1beta production in cultured rat microglia.
Microglia plays an important role in mediating neuroinflammation in Alzheimer's disease (AD). Intervention in microglia activation may exert a neuroprotective effect. In the present study, we reported that oligomeric Abeta1-42 dramatically increased the level of tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta compared to monomeric and fibrillar Abeta1-42 in rat microglial cultures. Pretreatment of the cultures with triptolide, an anti-inflammatory reagent, alleviated the elevation of TNF-alpha and IL-1beta level induced by oligomeric Abeta1-42. Our results showed that oligomeric Abeta played an important role in mediating neuroinflammation and triptolide was able to suppress the production of pro-inflammatory cytokines from microglia.
Jiao
Jian
J
Neuroscience Research Institute and Department of Neurobiology, Peking University, Key Laboratory for Neuroscience of the Ministry of Education, 38 Xueyuan Road, Beijing 100083, PR China.
Xue
Bing
B
Zhang
Lei
L
Gong
Yuntao
Y
Li
Kairong
K
Wang
Haomin
H
Jing
Liming
L
Xie
Junxia
J
Wang
Xiaomin
X
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004852:Epoxy Compounds / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism*; D017628:Microglia / Q000187:drug effects*; D010446:Peptide Fragments / Q000494:pharmacology*; D010616:Phenanthrenes / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D004224:Diterpenes; D004852:Epoxy Compounds; D007166:Immunosuppressive Agents; D053583:Interleukin-1beta; D010446:Peptide Fragments; D010616:Phenanthrenes; D014409:Tumor Necrosis Factor-alpha; C075222:amyloid beta-protein (1-42); C001899:triptolide