Methods to study monocyte migration induced by HIV-infected cells.
HIV-associated dementia (HAD) is a multi-factorial disease set in motion by the presence of HIV-infected cells in the brain. A characteristic feature of HAD is the infiltration of mononuclear phagocytes into the brain, which is aided by HIV-1 Tat protein and other chemokines secreted by both HIV-infected cells and uninfected cells in their vicinity. Both direct and indirect chemokine activity of HIV-1 Tat protein has been demonstrated employing purified recombinant Tat protein. However, a corroboration of a key role for Tat or other chemokines in monocyte migration, in the context of HIV-infection, has not yet been demonstrated. Here we describe methods, to measure the role of soluble factors, such as chemokines and Tat, released by HIV-infected cells or uninfected cells in their vicinity, in monocyte migration in vitro.
Rao
Vasudev R
VR
Department of Microbiology, Albert Einstein College of Medicine, Bronx, NY, USA.
Eugenin
Eliseo A
EA
Berman
Joan W
JW
Prasad
Vinayaka R
VR
D002460:Cell Line; D054441:Cell Migration Assays, Leukocyte / Q000379:methods*; D002465:Cell Movement; D002478:Cells, Cultured; D018925:Chemokines / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000276:immunology
D018925:Chemokines; D054322:tat Gene Products, Human Immunodeficiency Virus