title: HDL remodeling during the acute phase response.
abstract: OBJECTIVE
  The purpose of this study was to examine the interactive action of serum amyloid A (SAA), group IIA secretory phospholipase A(2) (sPLA(2)-IIA), and cholesteryl ester transfer protein (CETP) on HDL remodeling and cholesterol efflux during the acute phase (AP) response elicited in humans after cardiac surgery.
  
  
  RESULTS
  Plasma was collected from patients before (pre-AP), 24 hours after (AP-1 d), and 5 days after cardiac surgery (AP-5 d). SAA levels were increased 16-fold in AP-1 d samples. The activity of sPLA(2)-IIA was increased from 77.7+/-38.3 U/mL (pre-AP) to 281.4+/-57.1 U/mL (AP-1 d; P<0.001). CETP mass and activity reduction was commensurate to the reduction of HDL cholesterol levels. The combined action of SAA, sPLA(2)-IIA, and CETP in vitro markedly remodeled HDL with the generation of lipid-poor apoA-I from both pre-AP and AP-1 d HDL. The net result of this remodeling was a relative preservation of ABCA1- and ABCG1-dependent cholesterol efflux during the acute phase response.
  
  
  CONCLUSIONS
  Our results show that the many and complex changes in plasma proteins during the acute phase response markedly remodel HDL with functional implications, particularly the relative retention of cholesterol efflux capacity.
authors.affiliation: Department of Internal Medicine, Division of Endocrinology and Molecular Medicine, University of Kentucky, Lexington, KY, USA. anisa.jahangiri@uky.edu
authors.forename: Anisa
authors.initials: A
authors.lastname: Jahangiri
authors.forename: Maria C
authors.initials: MC
authors.lastname: de Beer
authors.forename: Victoria
authors.initials: V
authors.lastname: Noffsinger
authors.forename: Lisa R
authors.initials: LR
authors.lastname: Tannock
authors.forename: Chandrashekar
authors.initials: C
authors.lastname: Ramaiah
authors.forename: Nancy R
authors.initials: NR
authors.lastname: Webb
authors.forename: Deneys R
authors.initials: DR
authors.lastname: van der Westhuyzen
authors.forename: Frederick C
authors.initials: FC
authors.lastname: de Beer
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000097:blood; D000210:Acute-Phase Reaction / Q000097:blood / Q000209:etiology*; D016632:Apolipoprotein A-I / Q000097:blood; D006348:Cardiac Surgical Procedures / Q000009:adverse effects*; D002784:Cholesterol / Q000097:blood*; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood; D002846:Chromatography, Affinity; D054501:Group II Phospholipases A2 / Q000097:blood; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008264:Macrophages / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D000685:Serum Amyloid A Protein / Q000378:metabolism; D013997:Time Factors
chemical_list: C577836:ABCA1 protein, human; C419353:ABCG1 protein, human; C505693:APOA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; D016632:Apolipoprotein A-I; C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins; D008075:Lipoproteins, HDL; D011994:Recombinant Proteins; D000685:Serum Amyloid A Protein; D002784:Cholesterol; D054501:Group II Phospholipases A2