Ion-exclusion/cation-exchange chromatographic determination of common inorganic ions in human saliva by using an eluent containing zwitterionic surfactant.
Ion-exclusion/cation-exchange chromatography with an eluent containing the bile salt-type zwitterionic surfactant CHAPS was performed in order to evaluate variations in anion (SO(4)(2-), NO(3)(-), and SCN(-)) and cation (Na(+), K(+), NH(4)(+), Mg(2+), and Ca(2+)) concentrations in human saliva. CHAPS prevents the adsorption of proteins to the stationary phase, i.e., weakly acidic cation-exchange resin, since it aggregates proteins without denaturing them. Addition of 1mM CHAPS to the eluent comprising 6mM tartaric acid and 7 mM 18-crown-6 yielded reproducible separations of anions and cations in protein-containing saliva. The resolutions of anions and cations were not significantly affected by the addition of CHAPS to the eluent. The concentrations of Na(+) and K(+) varied before and after meals; or that of SCN(-), upon smoking. The relative standard deviations of peak areas ranged from 0.3 to 5.1% in 1 day (n=20) and from 1.4 to 5.8% over 6 days (n=6).
Mori
Masanobu
M
Graduate School of Engineering, Gunma University, Kiryu, Gunma, Japan. m_mori@chem-bio.gunma-u.ac.jp
Iwata
Tomotaka
T
Satori
Tatsuya
T
Ohira
Shin-Ichi
S
Itabashi
Hideyuki
H
Tanaka
Kazuhiko
K
D000838:Anions / Q000032:analysis*; D002118:Calcium / Q000032:analysis; D002412:Cations / Q000032:analysis*; D002793:Cholic Acids / Q000737:chemistry; D002850:Chromatography, Gel / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D008274:Magnesium / Q000032:analysis; D009566:Nitrates / Q000032:analysis; D011188:Potassium / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D012463:Saliva / Q000737:chemistry*; D012907:Smoking; D012964:Sodium / Q000032:analysis; D013431:Sulfates / Q000032:analysis; D013861:Thiocyanates / Q000032:analysis
D000838:Anions; D002412:Cations; D002793:Cholic Acids; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D013861:Thiocyanates; D012964:Sodium; D008274:Magnesium; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate; D011188:Potassium; D002118:Calcium