Ionizing radiation sensitizes leukemic MOLT-4 cells to TRAIL-induced apoptosis.
One of perspective approaches in treatment of hematological malignancies is activation of death receptors for TRAIL. However, leukemia cells studied to date have shown variable susceptibility to TRAIL. Our study demonstrates that cells of acute T-lymphoblastic leukemia MOLT-4 are resistant to TRAIL and that ionizing radiation in the therapeutically achievable dose of 1 Gy sensitizes TRAIL-resistant cells MOLT-4 to the TRAIL-induced apoptosis by increase in death receptors for TRAIL DR5. When TRAIL is applied after the irradiation in the time of increased DR5 positivity more efficient cell killing is achieved.
Rezácová
Martina
M
Charles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Medical Biochemistry, Czech Republic. rezacovam@lfhk.cuni.cz
Vávrová
Jirina
J
Vokurková
Doris
D
D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D005720:Gamma Rays; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D011836:Radiation Tolerance / Q000187:drug effects*; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects
D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D053221:TNF-Related Apoptosis-Inducing Ligand