title: A new abietane diterpenoid from Isodon inflexus.
abstract: A new ent-abietane diterpenoid, 3alpha,6beta-dihydroxy-7,17-dioxo-ent-abieta-15(16)-ene (1), and three known ent-kaurane diterpenids, kamebacetal A (2), kamebakaurin (3), and excisanin A (4), and a known triterpenoid, ursolic acid (5), were isolated from the aerial parts of Isodon inflexus. Their chemical structures were determined by extensive analysis of spectroscopic data including 1D-and 2D-NMR experiments. All isolates (1-5) were evaluated for their potential to inhibit LPS-induced nitric oxide production in RAW264.7 cells. Of these, compounds 1-4 inhibited the production of NO with IC(50) values ranging from 1.0 to 26.5 microM.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Chul
authors.initials: C
authors.lastname: Lee
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Hong
authors.forename: Xiang Hua
authors.initials: XH
authors.lastname: Han
authors.forename: Qinghao
authors.initials: Q
authors.lastname: Jin
authors.forename: Dayu
authors.initials: D
authors.lastname: Li
authors.forename: Tae Ook
authors.initials: TO
authors.lastname: Kim
authors.forename: Hyun-Kyu
authors.initials: HK
authors.lastname: Kim
authors.forename: Jeongrai
authors.initials: J
authors.lastname: Lee
authors.forename: Suk Hyung
authors.initials: SH
authors.lastname: Kwon
authors.forename: Yun-Bae
authors.initials: YB
authors.lastname: Kim
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
mesh_terms: D045784:Abietanes / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D037602:Isodon / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: C534002:3,6-dihydroxy-7,17-dioxo-ent-abieta-15(16)-ene; D045784:Abietanes; D004224:Diterpenes; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; D009569:Nitric Oxide; C022616:thiazolyl blue