title: Crystallization and preliminary X-ray analysis of the NKX2.5 homeodomain in complex with DNA.
abstract: As part of an effort to elucidate the molecular basis for the pathogenesis of NKX2.5 mutations in congenital heart disease using X-ray crystallography, the NKX2.5 homeodomain has been crystallized in complex with a specific DNA element, the -242 promoter region of atrial natriuretic factor. Crystals of the homeodomain-DNA complex diffracted X-rays to 1.7 A resolution and belonged to space group P6(5), with unit-cell parameters a = b = 71.5, c = 94.3 A. The asymmetric unit contained two molecules of the NKX2.5 homeodomain and one double-stranded oligonucleotide.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Caroli
authors.initials: C
authors.lastname: Genis
authors.forename: Peyton
authors.initials: P
authors.lastname: Scone
authors.forename: Hideko
authors.initials: H
authors.lastname: Kasahara
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Nam
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics; D003460:Crystallization; D004247:DNA / Q000737:chemistry*; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins; D046911:Macromolecular Substances; C095552:NKX2-5 protein, human; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D009320:Atrial Natriuretic Factor; D004247:DNA