Preparation and characterization of bisphenol A-cationized bovine serum albumin.
To detect BPA residue in the environment quickly by immune method, preparation of high quality antibody against BPA was the most critical step of project. Based on Mannich-type reaction and EDC method, we produced BPA-cBSA and BHPVA-BSA against BPA and the conjugates were characterized by UV-Vis and FT-IR spectroscopy. Compared with BSA, cBSA as carrier protein could improve the efficiency of coupling and significantly enhance the immune response against BPA. The mole coupling ratio of BPA-cBSA (8:1) was greater than that of BHPVA-BSA (5:1). We also observed that the similar sensitivity of antisera against BPA between the BPA-cBSA group and BHPVA-BSA group.
Feng
Yi
Y
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Zhou
Youxiang
Y
Zou
Qiang
Q
Wang
Jixing
J
Chen
Fusheng
F
Gao
Zhixian
Z
D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D001559:Benzhydryl Compounds; D002412:Cations / Q000737:chemistry; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry*; D013053:Spectrophotometry; D000098826:Bisphenol A Compounds
D000906:Antibodies; D001559:Benzhydryl Compounds; D002412:Cations; D010636:Phenols; D012710:Serum Albumin, Bovine; C006780:bisphenol A; D000098826:Bisphenol A Compounds