title: Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor.
abstract: A process was developed for producing human menin from transformed Drosophila Schneider 2 cells. Protein expression was achieved after inducing the metallothionein promoter by adding copper sulfate to cells growing in suspension in a stirred-tank reactor. Experiments in shake flasks showed that the production of menin was improved when the induction was conducted late in the exponential phase of cell growth at a concentration of 1-2 x 10(7) cells ml(-1), with a copper concentration of 0.2 mM for no more than 24 h. This observation was confirmed by experiments in bench-scale fermentors. Subsequently, a pilot-scale fermentation yielded 1 mg l(-1) culture of purified menin.
authors.affiliation: Biotechnology Unit, NIDDK, NIH, Bethesda, MD, 20892, U.S.A..
authors.forename: M A
authors.initials: MA
authors.lastname: Valle
authors.forename: M B
authors.initials: MB
authors.lastname: Kester
authors.forename: A L
authors.initials: AL
authors.lastname: Burns
authors.forename: S J
authors.initials: SJ
authors.lastname: Marx
authors.forename: A M
authors.initials: AM
authors.lastname: Spiegel
authors.forename: J
authors.initials: J
authors.lastname: Shiloach