title: Vitamin D3 deficiency enhances contact hypersensitivity in male but not in female mice.
abstract: To ascertain the influence of vitamin D3 and its metabolites on the function of the skin immune system and the induction of the contact hypersensitivity (CHS) response, a population of vitamin D3-deficient BALB/c mice was established, through dietary vitamin D3 restriction and limitation of exposure to UVB irradiation. Vitamin D3 normal female mice had higher CHS responses than their male counterparts, and dietary vitamin D3 deficiency significantly increased the CHS responses in male, but not in female, mice. This change in the vitamin D3-deficient male mice was not due to an alteration in skin dendritic cell function including antigen carriage, migration or costimulatory molecule expression. In addition, 18 h after sensitisation, the lymph node populations in the vitamin D3-deficient and normal male mice showed similar proliferation and IFN-gamma production. However, during the sensitisation phase of CHS, there was lower lymphocyte recruitment to the skin draining lymph nodes of the vitamin D3-deficient and normal male mice compared with their female counterparts which could account for the difference between the sexes in the extent of the CHS response. These results indicate the vitamin D system can influence cutaneous immune responses in male mice, but this did not occur through the modulation of the dendritic cell functions analysed.
authors.affiliation: Menzies Research Institute, Clinical School, University of Tasmania, Private Bag 29, Hobart, Tasmania 7000, Australia. r_malley@utas.edu.au
authors.forename: R C
authors.initials: RC
authors.lastname: Malley
authors.forename: H K
authors.initials: HK
authors.lastname: Muller
authors.forename: M
authors.initials: M
authors.lastname: Norval
authors.forename: G M
authors.initials: GM
authors.lastname: Woods
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000941:Antigens / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D002762:Cholecalciferol / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology*; D004032:Diet; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010081:Oxazolone / Q000276:immunology; D012867:Skin / Q000276:immunology* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014808:Vitamin D Deficiency / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; D016207:Cytokines; D010081:Oxazolone; D002762:Cholecalciferol; D007371:Interferon-gamma