Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates.
The e antigen (eAg) of duck hepatitis B virus (DHBV) is a glycosylated secretory protein with a currently unknown function. We concentrated this antigen from the supernatants of persistently infected primary duck liver cell cultures by ammonium sulphate precipitation, adsorption chromatography over concanavalin A Sepharose, preparative isoelectric focusing and molecular sieve chromatography. The combined treatment of duck liver cells with DHBV eAg (DHBe) concentrate and alpha-methyl-d-mannopyranoside strongly inhibited DHBV replication at de novo infection. When DHBe was added to non-infected primary duck liver cells, it was found to be associated with liver sinusoidal endothelial cells. This binding could be inhibited by the addition of alpha-methyl-d-mannopyranoside and other sugar molecules. The inhibitory effect of DHBe on infection could play a role in maintaining viral persistence.
Maenz
Claudia
C
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, 20251 Hamburg, Germany.
Loscher
Christine
C
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, 20251 Hamburg, Germany.
Iwanski
Alicja
A
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, 20251 Hamburg, Germany.
Bruns
Michael
M
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, 20251 Hamburg, Germany.
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002478:Cells, Cultured; D004372:Ducks; D018347:Hepadnaviridae Infections / Q000821:virology; D015611:Hepatitis B Virus, Duck / Q000187:drug effects* / Q000472:pathogenicity* / Q000502:physiology; D006513:Hepatitis B e Antigens / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006524:Hepatitis, Viral, Animal / Q000821:virology; D022781:Hepatocytes / Q000821:virology*; D008099:Liver / Q000166:cytology / Q000821:virology; D008766:Methylmannosides / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D002241:Carbohydrates; D006513:Hepatitis B e Antigens; D008766:Methylmannosides