title: Non-coding RNAs revealed during identification of genes involved in chicken immune responses.
abstract: Recent large-scale cDNA cloning studies have shown that a significant proportion of the transcripts expressed from vertebrate genomes do not appear to encode protein. Moreover, it was reported in mammals (human and mice) that these non-coding transcripts are expressed and regulated by mechanisms similar to those involved in the control of protein-coding genes. We have produced a collection of cDNA sequences from immunologically active tissues with the aim of discovering chicken genes involved in immune mechanisms, and we decided to explore the non-coding component of these immune-related libraries. After finding known non-coding RNAs (miRNA, snRNA, snoRNA), we identified new putative mRNA-like non-coding RNAs. We characterised their expression profiles in immune-related samples. Some of them showed changes in expression following viral infections. As they exhibit patterns of expression that parallel the behaviour of protein-coding RNAs in immune tissues, our study suggests that they could play an active role in the immune response.
authors.affiliation: CNRS, FRE2937, 7 rue Guy Moquet, F-94800, Villejuif, France. endale@vjf.cnrs.fr
authors.forename: Marie-Laure Endale
authors.initials: ML
authors.lastname: Ahanda
authors.forename: Thomas
authors.initials: T
authors.lastname: Ruby
authors.forename: Håkan
authors.initials: H
authors.lastname: Wittzell
authors.forename: Bertrand
authors.initials: B
authors.lastname: Bed'Hom
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Chaussé
authors.forename: Veronique
authors.initials: V
authors.lastname: Morin
authors.forename: Anne
authors.initials: A
authors.lastname: Oudin
authors.forename: Catherine
authors.initials: C
authors.lastname: Chevalier
authors.forename: John R
authors.initials: JR
authors.lastname: Young
authors.forename: Rima
authors.initials: R
authors.lastname: Zoorob
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000235:genetics / Q000276:immunology; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D008213:Lymphocyte Activation; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D008297:Male; D008380:Marek Disease / Q000235:genetics / Q000276:immunology; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D022661:RNA, Untranslated / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs; D022661:RNA, Untranslated