title: Application of calcium alginate-starch entrapped bitter gourd (Momordica charantia) peroxidase for the removal of colored compounds from a textile effluent in batch as well as in continuous reactor.
abstract: Calcium alginate-starch entrapped bitter gourd peroxidase has been employed for the treatment of a textile industrial effluent in batch as well as in continuous reactor. The textile effluent was recalcitrant to decolorization by bitter gourd peroxidase; thus, its decolorization was examined in the presence of a redox mediator, 1.0 mM 1-hydroxybenzotriazole. Immobilized enzyme exhibited same pH and temperature optima for effluent decolorization as attained by soluble enzyme. Immobilized enzyme could effectively remove more than 70% of effluent color in a stirred batch process after 3 h of incubation. Entrapped bitter gourd peroxidase retained 59% effluent decolorization reusability even after its tenth repeated use. The two-reactor system containing calcium alginate-starch entrapped enzyme retained more than 50% textile effluent decolorization efficiency even after 2 months of its operation. The absorption spectra of the treated effluent exhibited a marked difference in the absorption at various wavelengths as compared to untreated effluent. The use of a two-reactor system containing immobilized enzyme and an adsorbent will be significantly successful for treating industrial effluents at large scale, and it will help in getting water free from aromatic pollutants.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Mahreen
authors.initials: M
authors.lastname: Matto
authors.forename: Rukhsana
authors.initials: R
authors.lastname: Satar
authors.forename: Qayyum
authors.initials: Q
authors.lastname: Husain
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D004396:Coloring Agents / Q000737:chemistry / Q000302:isolation & purification*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000517:prevention & control*; D029777:Momordica charantia / Q000201:enzymology*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D013696:Temperature; D013783:Textile Industry; D018508:Water Purification / Q000379:methods
chemical_list: D000464:Alginates; D004396:Coloring Agents; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D007220:Industrial Waste; D020723:Glucuronic Acid; D013213:Starch; D009195:Peroxidase