Two cases of recovery of dimorphic pathogenic fungi via conventional BacT/ALERT microbial detection system media.
We hereby report two instances of dimorphic fungus cultivation in BacT/ALERT-based bacteriologic media, with the first such characterization of Blastomyces dermatitidis. From a patient with disseminated coccidioidomycosis, routine blood cultures incubated on the MB/BacT 3D Microbial Detection System generated a positive signal following 75 h of incubation. B. dermatitidis was isolated from a patient hospitalized with a four-week course of respiratory illness. Organism detection from respiratory specimens via the MB/BacT 3D Mycobacteria Detection System occurred 5 days sooner than the routine fungus culture. Etiologic agents of endemic mycoses may be isolated in bacteriologic media employed by continuous monitoring instrumentation.
Munson
Erik
E
Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, WI 53226, USA. Erik.Munson@wfhc.org
Endes
Tina
T
Vaughan
Katharine
K
Block
Timothy
T
Hryciuk
Jeanne E
JE
D000328:Adult; D001431:Bacteriological Techniques / Q000295:instrumentation*; D001758:Blastomyces / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D001759:Blastomycosis / Q000175:diagnosis / Q000382:microbiology; D001769:Blood / Q000382:microbiology*; D003045:Coccidioides / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D003047:Coccidioidomycosis / Q000175:diagnosis / Q000382:microbiology; D003470:Culture Media; D016469:Fungemia / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D008172:Lung Diseases, Fungal / Q000175:diagnosis / Q000382:microbiology; D008297:Male; D012137:Respiratory System / Q000382:microbiology*; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods; D013997:Time Factors
D003470:Culture Media