Fluorescence diffuse tomography for detection of red fluorescent protein expressed tumors in small animals.
A fluorescence diffuse tomography (FDT) setup for monitoring tumor growth in small animals has been created. In this setup an animal is scanned in the transilluminative configuration by a single source and detector pair. To remove stray light in the detection system, we used a combination of interferometric and absorption filters. To reduce the scanning time, an experimental animal was scanned using the following algorithm: (1) large-step scanning to obtain a general view of the animal (source and detector move synchronously); (2) selection of the fluorescing region; and (3) small-step scanning of the selected region and different relative shifts between the source and detector to obtain sufficient information for 3D reconstruction. We created a reconstruction algorithm based on the Holder norm to estimate the fluorophore distribution. This algorithm converges to the solution with a minimum number of fluorescing zones. The use of tumor cell lines transfected with fluorescent proteins allowed us to conduct intravital monitoring studies. Cell lines of human melanomas Mel-P, Mel-Ibr, Mel-Kor, and human embryonic kidney HEK293 Phoenix were transfected with DsRed-Express and Turbo-RFP genes. The emission of red fluorescent proteins (RFPs) in the long-wave optical range permits detection of deep-seated tumors. In vivo experiments were conducted immediately after subcutaneous injection of fluorescing cells into small animals.
Turchin
Ilya V
IV
Institute of Applied Physics RAS, 46 Ulyanov Street, 603950 Nizhny Novgorod, Russia. ilya@ufp.appl.sci-nnov.ru
Kamensky
Vladislav A
VA
Plehanov
Vladimir I
VI
Orlova
Anna G
AG
Kleshnin
Mikhail S
MS
Fiks
Ilya I
II
Shirmanova
Marina V
MV
Meerovich
Irina G
IG
Arslanbaeva
Lyaisan R
LR
Jerdeva
Viktoria V
VV
Savitsky
Alexander P
AP
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D008164:Luminescent Proteins; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D009369:Neoplasms / Q000473:pathology*; D041622:Tomography, Optical / Q000379:methods*; D051598:Whole Body Imaging / Q000379:methods*
D008164:Luminescent Proteins; C413663:fluorescent protein 583