Enzymes containing porous polymersomes as nano reaction vessels for cascade reactions.
Polystyrene(40)-b-poly(isocyanoalanine(2-thiophen-3-yl-ethyl)amide)(50) (PS-PIAT) polymersomes have the unique property of being sufficiently porous to allow diffusion of small (organic) substrates while retaining large biomolecules such as enzymes inside. Herein we report on the encapsulation and protection of glucose oxidase (GOx) and horse radish peroxidase (HRP) in PS-PIAT polymersomes and the successful employment of these functionalised nanoreactors in a cascade reaction. The demonstrated concept allows for further application in other enzymatic cascade reactions, bio-organic hybrid systems and biosensing devices.
Kuiper
Suzanne M
SM
Molecular Materials, Institute for Molecules and Materials, University of Nijmegen, Toernooiveld 1, Nijmegen, Gelderland, Netherlands 6500 GL.
Nallani
Madhavan
M
Vriezema
Dennis M
DM
Cornelissen
Jeroen J L M
JJ
van Hest
Jan C M
JC
Nolte
Roeland J M
RJ
Rowan
Alan E
AE
D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D005949:Glucose Oxidase / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007097:Imines / Q000737:chemistry*; D036103:Nanotechnology; D011137:Polystyrenes / Q000737:chemistry*; D016062:Porosity; D013997:Time Factors
D007097:Imines; D011137:Polystyrenes; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D000409:Alanine