title: Mannosylerythritol lipid increases levels of galactoceramide in and neurite outgrowth from PC12 pheochromocytoma cells.
abstract: We report here that a microbial extracellular glycolipid,mannosylerythritol lipid (MEL), induces the outgrowth ofneurites from and enhances the activity of acetylcholinesterase(AChE) in PC12 pheochromocytoma cells. Furthermore, treatment ofPC12 cells with MEL increased levels of galactosylceramide(Galbeta1-1'Cer; GalCer). Exposure of PC12 cells to exogenous GalCer caused the dose-dependent outgrowth ofneurites. By contrast, treatment of PC12 cells with nerve growthfactor (NGF) did not increase the level of GalCer in the cells. The neurite-related morphological changes induced by GalCerdifferend from those induced by NGF, indicating differencesbetween the signal transduction pathways triggered by NGF and by GalCer.
authors.affiliation: Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shibahara
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Wakamatsu
authors.forename: N
authors.initials: N
authors.lastname: Nomura
authors.forename: T
authors.initials: T
authors.lastname: Nakahara
authors.forename: C
authors.initials: C
authors.lastname: Jin
authors.forename: H
authors.initials: H
authors.lastname: Nagaso
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.forename: K K
authors.initials: KK
authors.lastname: Yokoyama