The selective Dectin-1 agonist, curdlan, induces an oxidative burst response in chicken heterophils and peripheral blood mononuclear cells.
A critical component of host innate immunity is recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs). Dectin-1 is the primary PRR for exogenous beta-glucan, a component of fungal and bacterial cell walls. A previous study conducted in our laboratory demonstrated that administration of beta-glucan as a feed additive resulted in increased innate immune function of neonatal chickens, suggesting that chickens possess a Dectin-1-like beta-glucan receptor. In the present study, we demonstrated that heterophils and peripheral blood mononuclear cells (PBMCs) from day-old chicks had a significant increase in the generation of reactive oxygen species (ROS) following stimulation with the Dectin-1 specific agonist, curdlan. Pretreatment of heterophils and PBMCs with laminarin, a beta-glucan receptor blocking agent and specific inhibitor of Dectin-1 activity, significantly reduced the curdlan-induced ROS production. Together these data provide evidence for the first time of the presence of a functional Dectin-1-like beta-glucan receptor in chicken heterophils and PBMCs.
Nerren
Jessica R
JR
USDA-ARS, Southern Plains Agricultural Research Center, College Station, TX 77845, USA. jessica.nerren@ars.usda.gov
Kogut
Michael H
MH
D000818:Animals; D000831:Animals, Newborn; D002645:Chickens / Q000097:blood* / Q000276:immunology; D005936:Glucans; D006098:Granulocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D007113:Immunity, Innate / Q000187:drug effects; D066298:In Vitro Techniques; D037181:Lectins, C-Type / Q000819:agonists / Q000037:antagonists & inhibitors / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D008565:Membrane Proteins / Q000819:agonists* / Q000037:antagonists & inhibitors / Q000097:blood; D009419:Nerve Tissue Proteins / Q000819:agonists* / Q000037:antagonists & inhibitors / Q000097:blood; D011134:Polysaccharides / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000097:blood; D011971:Receptors, Immunologic / Q000819:agonists / Q000037:antagonists & inhibitors / Q000097:blood; D016897:Respiratory Burst / Q000187:drug effects*; D047071:beta-Glucans / Q000494:pharmacology*; D000099181:Dectin-1
D005936:Glucans; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D011971:Receptors, Immunologic; D047071:beta-Glucans; D000099181:Dectin-1; C045268:beta-glucan receptor; C038459:curdlan; C008247:laminaran