Simple method to accurately differentiate Candida albicans isolates concurrently using polymorphic patterns of PCR-amplified, species-specific nuclear and mitochondrial targets.
We describe a simple method to accurately differentiate Candida albicans isolates by concurrent use of the restriction enzyme digestion patterns for PCR products, targeting two species-specific DNA regions originating from genetically different sources, the nuclear and mitochondrial genomes. The target sequence we used as the nuclear gene was derived from the PHO85 gene, a negative regulator of the PHO system, in which we found a restriction size polymorphism within the two alleles of PHO85 in the diploid genome of this fungus. The mitochondrial target was derived from EO3, a species-specific DNA fragment possessing a small size polymorphism among various clinical isolates. Our results should provide a new tool for molecular epidemiological surveys of patients suffering from candidiasis caused by C. albicans.
Miyakawa
Yozo
Y
Division of Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takada, Kofu, Japan. ymiya@yamanashi.ac.jp
Ozawa
Takuya
T
D002176:Candida albicans / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D002467:Cell Nucleus / Q000235:genetics*; D018844:Cyclin-Dependent Kinases / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D008928:Mitochondria / Q000235:genetics*; D016533:Mycological Typing Techniques / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013045:Species Specificity
D005656:Fungal Proteins; D018844:Cyclin-Dependent Kinases