Substitution of certain amino acids in a short peptide causes a significant difference in their immunoreactivities with antibodies against different epitopes: evidence for possible folding of the peptide on a nitrocellulose or PVDF membrane.
Substitution of amino acids in a peptide caused remarkable differences in its immunoreactivities with antibodies against 3 epitopes in the immobilized peptide. The observed differences in immunoreactivities among the peptides were not due to the differences in efficiencies of their transfer onto nitrocellulose or PVDF membranes. Rather, possible folding of the peptide on the membrane was considered to be the reason for their distinct immunoreactivities with the antibodies.
Matsuo
Taisuke
T
Institute for Genome Research, University of Tokushima, Kuramotocho-3, Tokushima 770-8503, Japan.
Yamamoto
Takenori
T
Katsuda
Chie
C
Niiyama
Kanami
K
Yamamoto
Atsushi
A
Yamazaki
Naoshi
N
Ohkura
Kazuto
K
Kataoka
Masatoshi
M
Shinohara
Yasuo
Y
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000502:physiology*; D000906:Antibodies / Q000276:immunology; D000937:Antigen-Antibody Reactions / Q000235:genetics / Q000276:immunology; D003101:Collodion / Q000737:chemistry / Q000494:pharmacology*; D000939:Epitopes / Q000737:chemistry / Q000235:genetics*; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics*; D011145:Polyvinyls / Q000737:chemistry / Q000494:pharmacology*; D017510:Protein Folding / Q000187:drug effects; D005465:Fluorocarbon Polymers
D000906:Antibodies; D003101:Collodion; D000939:Epitopes; D008567:Membranes, Artificial; D010446:Peptide Fragments; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers