Thioglycolic acid inhibits mouse oocyte maturation and affects chromosomal arrangement and spindle configuration.
Previous studies have shown that thioglycolic acid (TGA) leads to potential reproductive toxicology. To clarify the exact effects of this compound on reproduction, mice oocytes were treated with different TGA doses. At the end of the culture period, the nuclear status of mice oocytes was assessed under an inverted microscope. After immunofluorescence staining, the chromosomal arrangement and spindle configuration of oocytes were evaluated. The results indicated that TGA decreases the percentage of first polar body formation but does not influence that of germinal vesicle breakdown. TGA induces abnormal chromosomal arrangement and spindle elongation. In conclusion, TGA inhibits in-vitro maturation of mice oocytes and affects chromosomal arrangement and spindle configuration. Furthermore, it probably interferes with biochemical changes that occur during meiosis, resulting in aberrant development.
Hou
S Y
SY
Department of Nutrition and Food Hygiene, Harbin Medical University, Heilongjiang, China.
Zhang
L
L
Wu
K
K
Xia
L
L
D000818:Animals; D033481:Chromosomes, Mammalian / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro; D005455:Fluorescent Antibody Technique; D006062:Gonadotropins / Q000008:administration & dosage; D066298:In Vitro Techniques; D008677:Metaphase / Q000187:drug effects; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D008941:Spindle Apparatus / Q000187:drug effects*; D013864:Thioglycolates / Q000008:administration & dosage / Q000633:toxicity*
D006062:Gonadotropins; D013864:Thioglycolates