Design and optimization of a high-speed, high-sensitivity, spinning disk confocal microscopy system.
We describe the principles, design, and systems integration of a flexible, high-speed, high-sensitivity, high-resolution confocal spinning disk microscopy (SDCM) system. We present several artifacts unique to high-speed SDCM along with techniques to minimize them. We show example experimental results from a specific implementation capable of generating 3-D image stacks containing 30 2-D slices at 30 stacks per second. This implementation also includes optics for differential interference contrast (DIC), phase, and bright-field imaging, as well as an optical trap with sensitive force and position measurement.
McAllister
Ryan G
RG
Georgetown University, Department of Physics, Washington, DC 20057, USA. rmca@physics.georgetown.edu
Sisan
Daniel R
DR
Urbach
Jeffrey S
JS
D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D018715:Microscopy, Video / Q000295:instrumentation*; D015203:Reproducibility of Results; D012399:Rotation; D012680:Sensitivity and Specificity