title: The purification and characterisation of allergenic hazelnut seed proteins.
abstract: A lipid transfer protein (LTP, Cor a 8) together with the 11S (Cor a 9) and 7S seed storage globulins (Cor a 11) are major food allergens present in hazelnut. Methods are described for their purification and characterisation using in-gel tryptic digestion mass spectrometry to confirm their identities and circular dichroism and Fourier-transform infrared spectroscopies to demonstrate that they are authentically folded. Preliminary immunochemical studies have also confirmed that the purified preparations retain their immunological properties in terms of immunoglobulin E binding, determined by immunoblotting using serum from hazelnut allergic patients. These preparations form a basis for development of improved methods of diagnosis of food allergy based on the concept of component-resolved diagnosis.
authors.affiliation: Institute of Food Research, Norwich, UK.
authors.forename: Neil M
authors.initials: NM
authors.lastname: Rigby
authors.forename: Justin
authors.initials: J
authors.lastname: Marsh
authors.forename: Ana I
authors.initials: AI
authors.lastname: Sancho
authors.forename: Klaus
authors.initials: K
authors.lastname: Wellner
authors.forename: Jaap
authors.initials: J
authors.lastname: Akkerdaas
authors.forename: Ronald
authors.initials: R
authors.lastname: van Ree
authors.forename: Andre
authors.initials: A
authors.lastname: Knulst
authors.forename: Montserrat
authors.initials: M
authors.lastname: Fernández-Rivas
authors.forename: Vlasta
authors.initials: V
authors.lastname: Brettlova
authors.forename: Piet P
authors.initials: PP
authors.lastname: Schilte
authors.forename: Colin
authors.initials: C
authors.lastname: Summer
authors.forename: Richard
authors.initials: R
authors.lastname: Pumphrey
authors.forename: Peter R
authors.initials: PR
authors.lastname: Shewry
authors.forename: E N Clare
authors.initials: EN
authors.lastname: Mills
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D031211:Corylus / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000378:metabolism; D008969:Molecular Sequence Data; D021184:Nut Hypersensitivity / Q000209:etiology*; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D017433:Protein Structure, Secondary; D012639:Seeds / Q000737:chemistry / Q000276:immunology*; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000485:Allergens; D010940:Plant Proteins; D007073:Immunoglobulin E