Following ionic activity by electrochemistry during the polymerase chain reaction.
The most commonly used technique for gene detection is the polymerase chain reaction (PCR). PCR is associated with alterations in ionic activity because inorganic pyrophosphate (PPi) and inorganic phosphate (Pi) ions are produced during nucleotide polymerization. To maintain electro-neutrality, magnesium, potassium, and ammonium ions are bound to DNA. Deoxynucleotides are also bound to DNA during PCR. Some authors have described DNA itself as an electrically conducting polymer formed by base stapling with the formation of extensive Pi systems. In the current study, alterations in electrical conductivity determined experimentally during PCR are reported, and a model explaining the observed changes is described. During recent years, several different techniques for quantifying PCR products have been developed. The most frequently used technique is comparison of the densitometric intensities of ethidium bromide-stained PCR products separated by electrophoresis on gels. Here an alternative technique for quantifying PCR products by measuring alterations in electrical conductivity during PCR is presented.
Arneth
Borros
B
Institute for Clinical Chemistry and Laboratory Medicine, Johannes Gutenberg University Mainz, 55131 Mainz, Germany. arneth@zentrallabor.klinik.uni-mainz.de
D002712:Chlorides / Q000032:analysis*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004553:Electric Conductivity; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D007477:Ions / Q000032:analysis; D008274:Magnesium / Q000032:analysis*; D010710:Phosphates / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011188:Potassium / Q000032:analysis*; D012313:RNA / Q000737:chemistry; D012964:Sodium / Q000032:analysis*; D013696:Temperature
D002712:Chlorides; D007477:Ions; D010710:Phosphates; D012313:RNA; D004247:DNA; D012964:Sodium; D008274:Magnesium; D011188:Potassium