In vivo expansion of cells expressing acquired CSF3R mutations in patients with severe congenital neutropenia.
Severe congenital neutropenia (CN) is a rare bone marrow failure syndrome with a high incidence of acute leukemia. In previous studies, we could show that point mutations in the gene for the granulocyte colony-stimulating factor (G-CSF) receptor CSF3R are a highly predictive marker for leukemic development in CN patients. To find out at which stage of hematopoietic development these mutations emerge and how they are propagated during hematopoietic differentiation, we analyzed single cells of different hematopoietic subpopulations from CN patients with CSF3R mutations. We found that CSF3R mutations are not restricted to the myeloid compartment but are also detectable in lymphoid cells, although at a much lower percentage. From our observations, we conclude that CSF3R mutations are acquired in multipotent hematopoietic progenitor cells in CN patients and that they are clonally expanded in myeloid cells expressing the G-CSF receptor due to the growth advantage mediated by the CSF3R mutation.
Germeshausen
Manuela
M
Department of Pediatric Hematology and Oncology, Hannover Medical School, Hannover, Germany. germeshausen.manuela@mh-hannover.de
Welte
Karl
K
Ballmaier
Matthias
M
D000293:Adolescent; D000328:Adult; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D018450:Disease Progression; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000235:genetics; D009154:Mutation; D009503:Neutropenia / Q000151:congenital / Q000235:genetics*; D011230:Precancerous Conditions / Q000235:genetics*; D016184:Receptors, Colony-Stimulating Factor / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C517047:CSF3R protein, human; D016184:Receptors, Colony-Stimulating Factor