title: Simultaneous determination of Fe(III) and Fe(II) ions via complexation with salicylic acid and 1,10-phenanthroline in microcolumn ion chromatography.
abstract: Fe(III) and Fe(II) ions were separated and determined via in-column and post-column reactions with salicylic acid and 1,10-phenanthroline by microcolumn ion chromatography with UV-Vis detection. The separation could be achieved on a silica microcolumn using an aqueous ethanol solution of salicylic acid as eluent. The effluent from the column was then mixed with aqueous ethanol solution of 1,10-phenanthroline and detected at 518 nm. Fe(III) ion was detected as the complex with salicylic acid, whereas Fe(II) ion was detected as the complex with 1,10-phenanthroline. The detection limits with 0.25 microL injection volume at S/N = 3 were 0.21 and 0.10 mg L(-1) for Fe(III) and Fe(II), respectively. Increasing the injection volume of sample, e.g., 5.1 microL, improved the sensitivity; the detection limits at S/N = 3 were 12 and 9.2 microg L(-1) for Fe(III) and Fe(II), respectively. The present system was applied to the determination of iron in a local well water sample.
authors.affiliation: Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Budhi
authors.initials: B
authors.lastname: Oktavia
authors.forename: Lee Wah
authors.initials: LW
authors.lastname: Lim
authors.forename: Toyohide
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D005290:Ferric Compounds / Q000032:analysis / Q000302:isolation & purification*; D005296:Ferrous Compounds / Q000032:analysis / Q000302:isolation & purification*; D005618:Fresh Water / Q000032:analysis; D010618:Phenanthrolines / Q000737:chemistry*; D020156:Salicylic Acid / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D010618:Phenanthrolines; D020156:Salicylic Acid