title: Simultaneous extraction and clean-up of polychlorinated biphenyls and their metabolites from small tissue samples using pressurized liquid extraction.
abstract: A pressurized liquid extraction-based method for the simultaneous extraction and in situ clean-up of polychlorinated biphenyls (PCBs), hydroxylated (OH)-PCBs and methylsulfonyl (MeSO(2))-PCBs from small (<0.5 g) tissue samples was developed and validated. Extraction of a laboratory reference material with hexane-dichloromethane-methanol (48:43:9, v/v) and Florisil as fat retainer allowed an efficient recovery of PCBs (78-112%; RSD: 13-37%), OH-PCBs (46+/-2%; RSD: 4%) and MeSO(2)-PCBs (89+/-21%; RSD: 24%). Comparable results were obtained with an established analysis method for PCBs, OH-PCBs and MeSO(2)-PCBs.
authors.affiliation: Department of Occupational and Environmental Health, Collage of Public Health, University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Izabela
authors.initials: I
authors.lastname: Kania-Korwel
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhao
authors.forename: Karin
authors.initials: K
authors.lastname: Norstrom
authors.forename: Xueshu
authors.initials: X
authors.lastname: Li
authors.forename: Keri C
authors.initials: KC
authors.lastname: Hornbuckle
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Lehmler
mesh_terms: D000818:Animals; D001140:Aroclors / Q000008:administration & dosage; D005591:Chemical Fractionation / Q000379:methods*; D020111:Chlorodiphenyl (54% Chlorine) / Q000008:administration & dosage; D016014:Linear Models; D008099:Liver / Q000737:chemistry*; D008297:Male; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000302:isolation & purification*; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry
chemical_list: D001140:Aroclors; D012997:Solvents; D020111:Chlorodiphenyl (54% Chlorine); C027423:aroclor 1242; D011078:Polychlorinated Biphenyls