title: Laser speckle imaging of atherosclerotic plaques through optical fiber bundles.
abstract: Laser speckle imaging (LSI), a new technique that measures an index of plaque viscoelasticity, has been investigated recently to characterize atherosclerotic plaques. These prior studies demonstrated the diagnostic potential of LSI for detecting high-risk plaques and were conducted ex vivo. To conduct intracoronary LSI in vivo, the laser speckle pattern must be transmitted from the coronary wall to the image detector in the presence of cardiac motion. Small-diameter, flexible optical fiber bundles, similar to those used in coronary angioscopy, may be incorporated into an intravascular catheter for this purpose. A key challenge is that laser speckle is influenced by inter-fiber leakage of light, which may be exacerbated during bundle motion. In this study, we tested the capability of optical fiber bundles to transmit laser speckle patterns obtained from atherosclerotic plaques and evaluated the influence of motion on the diagnostic accuracy of fiber bundle-based LSI. Time-varying helium-neon laser speckle images of aortic plaques were obtained while cyclically moving the flexible length of the bundle to mimic coronary motion. Our results show that leached fiber bundles may reliably transmit laser speckle images in the presence of cardiac motion, providing a viable option to conduct intracoronary LSI.
authors.affiliation: Harvard Medical Schooland Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
authors.forename: Seemantini K
authors.initials: SK
authors.lastname: Nadkarni
authors.forename: Brett E
authors.initials: BE
authors.lastname: Bouma
authors.forename: Dvir
authors.initials: D
authors.lastname: Yelin
authors.forename: Amneet
authors.initials: A
authors.lastname: Gulati
authors.forename: Guillermo J
authors.initials: GJ
authors.lastname: Tearney
mesh_terms: D050197:Atherosclerosis / Q000473:pathology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D007834:Lasers; D019047:Phantoms, Imaging; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity