Shh and ROCK1 modulate the dynamic epithelial morphogenesis in circumvallate papilla development.
In rodents, a circumvallate papilla (CVP) develops with dynamic changes in epithelial morphogenesis during early tongue development. Molecular and cellular studies of CVP development revealed that there would be two different mechanisms in the apex and the trench wall forming regions with specific expression patterns of Wnt11 and Shh. Molecular interactions were examined using in vitro organ culture with over-expression of Shh, important signalling molecules and various inhibitors revealed that there are two significant different mechanisms in CVP formation by Wnt11 and Shh expressions. Wnt, a well known key molecule to initiate taste papillae, would govern Rho activation and cytoskeleton formation in the apex epithelium of CVP. In contrast, Shh regulates the cell proliferation to differentiate taste buds and to invaginate the epithelium for development of von Ebner's gland (VEG). Based on these results, we suggest that these different molecular signalling cascades of Wnt11 and Shh would play crucial roles in specific morphogenesis and pattern formation of CVP during early mouse embryo development.
Kim
Jae-Young
JY
Department of Biochemistry, School of Dentistry, Brain Korea 21 project, IHBR, Kyungpook National University, Daegu, Republic of Korea.
Lee
Min-Jung
MJ
Cho
Kyoung-Won
KW
Lee
Jong-Min
JM
Kim
Yeun-Jung
YJ
Kim
Ji-Youn
JY
Jung
Hye-In
HI
Cho
Je-Yoel
JY
Cho
Sung-Won
SW
Jung
Han-Sung
HS
D000577:Amides / Q000494:pharmacology; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects / Q000196:embryology* / Q000201:enzymology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D053823:Hedgehog Proteins / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D008954:Models, Biological; D009024:Morphogenesis / Q000187:drug effects; D009924:Organ Culture Techniques; D011725:Pyridines / Q000494:pharmacology; D014059:Tongue / Q000166:cytology / Q000196:embryology* / Q000201:enzymology*; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000577:Amides; D053823:Hedgehog Proteins; D011725:Pyridines; C465570:Shh protein, mouse; C108830:Y 27632; C515854:Rock1 protein, mouse; D054460:rho-Associated Kinases