title: Phenotypic and genotypic characterization of Arcanobacterium haemolyticum isolates from infections of horses.
abstract: The present study was designed to characterize phenotypically and genotypically seven Arcanobacterium haemolyticum strains obtained from infections of six horses. All seven strains showed the cultural and biochemical properties typical of A. haemolyticum and were susceptible to most of the antibiotics tested. The species identification could be confirmed by amplification and sequencing of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region and by PCR amplification of species-specific parts of the gene encoding phospholipase D in A. haemolyticum. Use of the latter could possibly improve future identification of this generally human pathogenic bacterial species which, according to the present results, seems to occur also in infections of horses.
authors.affiliation: De Gezondheidsdienst voor Dieren, Postbus 9, 7400 AA Deventer, The Netherlands.
authors.forename: Abdulwahed Ahmed
authors.initials: AA
authors.lastname: Hassan
authors.forename: Hivda
authors.initials: H
authors.lastname: Ulbegi-Mohyla
authors.forename: Talah
authors.initials: T
authors.lastname: Kanbar
authors.forename: Jörg
authors.initials: J
authors.lastname: Alber
authors.forename: Christoph
authors.initials: C
authors.lastname: Lämmler
authors.forename: Amir
authors.initials: A
authors.lastname: Abdulmawjood
authors.forename: Michael
authors.initials: M
authors.lastname: Zschöck
authors.forename: Reinhard
authors.initials: R
authors.lastname: Weiss
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D055667:Arcanobacterium / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000235:genetics; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011817:Rabbits; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010739:Phospholipase D