title: An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening.
abstract: Glucuronic acid is a common chemical moiety that decorates the xylan polymer of hemicellulose. This chemical substituent impairs both enzymatic and acidic hydrolysis of xylosidic bonds. The alpha-glucuronidase enzyme hydrolyzes the 1,2-linked glucuronic acid from the terminal, non-reducing xylose of xylo-oligosaccharides. There are relatively few alpha-glucuronidase genes in the public databases. We have developed an assay with commercially available reagents that can be used to search DNA libraries for alpha-glucuronidase genes in a high-throughput, solid phase activity screen.
authors.affiliation: USDA-ARS, Albany, CA 94710, USA. CLEE@pw.usda.gov
authors.forename: Charles C
authors.initials: CC
authors.lastname: Lee
authors.forename: Kurt
authors.initials: K
authors.lastname: Wagschal
authors.forename: Rena E
authors.initials: RE
authors.lastname: Kibblewhite-Accinelli
authors.forename: William J
authors.initials: WJ
authors.lastname: Orts
authors.forename: George H
authors.initials: GH
authors.lastname: Robertson
authors.forename: Dominic W S
authors.initials: DW
authors.lastname: Wong
mesh_terms: D001681:Biological Assay / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D020723:Glucuronic Acid / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*
chemical_list: D020723:Glucuronic Acid; D006026:Glycoside Hydrolases; C096734:alpha-glucuronidase