Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma.
Gadd45, MyD118 and CR6 (also termed Gadd45alpha, Gadd45beta and Gadd45gamma, respectively) comprise a family of proteins that play important roles in negative growth control, maintenance of genomic stability, DNA repair, cell-cycle control and apoptosis. Recombinant human Gadd45gamma and its selenomethionine derivative were expressed in an Escherichia coli expression system and purified; they were then crystallized using the hanging-drop vapour-diffusion method. Diffraction-quality crystals were grown at 291 K using PEG 3350 as precipitant. Using synchrotron radiation, the best diffraction data were collected to 2.3 A resolution for native crystals at 100 K; selenomethionyl derivative data were collected to 3.3 A resolution. All the crystals belonged to space group I2(1)3, with approximate unit-cell parameters a = b = c = 126 A.
Zhang
Wenzheng
W
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Zhao
Mingzhuo
M
Li
Jianhui
J
Li
Xuemei
X
Zeng
ZongHao
Z
Rao
Zihe
Z
D003460:Crystallization; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012645:Selenomethionine / Q000737:chemistry; D017356:Synchrotrons; D014961:X-Ray Diffraction; D000097642:GADD45 Proteins
D047908:Intracellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D012645:Selenomethionine; D000097642:GADD45 Proteins