title: Induction of Mac-2BP by nerve growth factor is regulated by the PI3K/Akt/NF-kappaB-dependent pathway in the HEK293 cell line.
abstract: Mac-2BP is a ligand of the galectin family that has been suggested to affect tumor proliferation and metastasis formation. We assessed Mac-2BP expression at the transcriptional and translational levels to evaluate nerve growth factor (NGF)-induced Mac-2BP expression. A time kinetic analysis using reverse transcription-polymerase chain reaction showed that NGF-induced Mac-2BP transcript levels were 4-5 times higher than in controls. Mac-2BP enzyme-linked immunosorbent assay and immunofluorescence staining showed a 2-3-fold increase in intracellular and secreted Mac-2BP as a result of NGF stimulation. This increase was regulated by Akt activation and NF-kappaB binding. p65 and p50-NF-kappaB are major transcriptional factors in the Mac-2BP promoter region, and were shown to be regulated in accordance with the Akt activation states. Collectively, these results suggest that NGF induces Mac-2BP expression via the PI3K/Akt/NF-kappaB pathway.
authors.affiliation: Stem Cell Research Center, KRIBB, Daejeon, Korea.
authors.forename: Yuk Pheel
authors.initials: YP
authors.lastname: Park
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Choi
authors.forename: Bo-Yeon
authors.initials: BY
authors.lastname: Kim
authors.forename: Jong-Tae
authors.initials: JT
authors.lastname: Kim
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Song
authors.forename: Seong Ho
authors.initials: SH
authors.lastname: Kang
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Yoon
authors.forename: Sang-Gi
authors.initials: SG
authors.lastname: Paik
authors.forename: Kwang Dong
authors.initials: KD
authors.lastname: Kim
authors.forename: Jong Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Hee Gu
authors.initials: HG
authors.lastname: Lee
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism / Q000502:physiology*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D051058:Oncogene Protein v-akt / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D011485:Protein Binding; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D015533:Transcriptional Activation / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D000951:Antigens, Neoplasm; D008562:Membrane Glycoproteins; D016328:NF-kappa B; C114782:TAA90K protein, human; D004247:DNA; D020932:Nerve Growth Factor; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt