[Molecular identification of medicinal plant genus Uncaria in Guizhou].
OBJECTIVE
To analyze rDNA ITS regions of the Medicinal Plant Genus Uncaria in Guizhou and construct their phylogenetic tree in order to supply molecular evidence of taxonomy and identification of these Medicinal Plants in genetic level.


METHODS
The ITS gene fragments of the 4 Medicinal Plants were PCR amplified and sequenced. The rDNA ITS regions were analyzed by means of the software of ClustalX, BioEdit and PAUP* 4.0 beta 10.


RESULTS
The entire sequences of rDNA ITS1, ITS2, and 5.8S rDNA were obtained, The Maximum-parsimony tree of four ITS regions together with those of similar sequences from GenBank were found, as Mitrayna rubrostipulata (AJ492621 ) and Mitragyna rubrostipulata (AJ605988) were designated as outgroup.


CONCLUSIONS
The 4 medicinal plants are the 4 species in the genus Uncaria, and are mostly similar to the Uncaria rhynhcophylla.
Gang
Tao
T
The Key Laboratory for Agri. Biotechnology of Guizhou, Guiyang 550006, China. ttg729@sina.com
Liu
Tao
T
Zhu
Ying
Y
Liu
Zuo-Yi
ZY
D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction / Q000379:methods; D018749:RNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D032070:Uncaria / Q000145:classification / Q000235:genetics* / Q000254:growth & development
D004275:DNA, Ribosomal; D018749:RNA, Plant