Enantioselective oxidation of racemic lactic acid to D-lactic acid and pyruvic acid by Pseudomonas stutzeri SDM.
D-lactic acid and pyruvic acid are two important building block intermediates. Production of D-lactic acid and pyruvic acid from racemic lactic acid by biotransformation is economically interesting. Biocatalyst prepared from 9 g dry cell wt l(-1) of Pseudomonas stutzeri SDM could catalyze 45.00 g l(-1)DL-lactic acid into 25.23 g l(-1)D-lactic acid and 19.70 g l(-1) pyruvic acid in 10h. Using a simple ion exchange process, D-lactic acid and pyruvic acid were effectively separated from the biotransformation system. Co-production of d-lactic acid and pyruvic acid by enantioselective oxidation of racemic lactic acid is technically feasible.
Gao
Chao
C
State Key Laboratory of Microbial Technology, Shandong University, Shandananlu 27, Jinan 250100, People's Republic of China.
Qiu
Jianhua
J
Li
Jingchen
J
Ma
Cuiqing
C
Tang
Hongzhi
H
Xu
Ping
P
D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D002384:Catalysis; D019344:Lactic Acid / Q000378:metabolism*; D010084:Oxidation-Reduction; D044223:Pseudomonas stutzeri / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism*
D019344:Lactic Acid; D019289:Pyruvic Acid