title: The C-terminal domains of adenovirus serotype 5 protein IX assemble into an antiparallel structure on the facets of the capsid.
abstract: Adenovirus serotype 5 protein IX (pIX) has two domains connected by a flexible linker. Three N-terminal domains form triskelions on the capsid facets that cement hexons together, and the C-terminal domains of four monomers form complexes toward the facet periphery. Here we present a cryoelectron microscopy structure of recombinant adenovirus with a peptide tag added to the C terminus of pIX. The structure, made up by several C termini of pIX, is longer at both ends than the wild-type protein, and Fabs directed against the tag bind to both ends of the oligomer, demonstrating that the pIX C termini associate in an antiparallel manner.
authors.affiliation: Université Joseph Fourier, Unit for Virus Host Cell Interaction, UMR 5233 UJF-EMBL-CNRS, Grenoble cedex 9, France.
authors.forename: Céline M S
authors.initials: CM
authors.lastname: Fabry
authors.forename: Manuel
authors.initials: M
authors.lastname: Rosa-Calatrava
authors.forename: Christine
authors.initials: C
authors.lastname: Moriscot
authors.forename: Rob W H
authors.initials: RW
authors.lastname: Ruigrok
authors.forename: Pierre
authors.initials: P
authors.lastname: Boulanger
authors.forename: Guy
authors.initials: G
authors.lastname: Schoehn
mesh_terms: D000256:Adenoviridae / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure*; D000914:Antibodies, Viral / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D020285:Cryoelectron Microscopy; D006801:Humans; D008958:Models, Molecular; D011485:Protein Binding; D020836:Protein Structure, Quaternary
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus