title: Giardia duodenalis: effects of an ozonized sunflower oil product (Oleozon) on in vitro trophozoites.
abstract: The ozonized sunflower oil product (Oleozon) was investigated to explore its cytotoxic activity on Giardia duodenalis in vitro cultivated trophozites. Oleozon produced inactivation of Giardia trophozoites in a dose- and cell density-dependent manner. Thirty microliter of Oleozon with peroxide index value of 500 equivalent-mmol of activated oxygen per kilogram were used to achieve a 100% inhibition (<-4.00 log unit) of trophozoites from an initial inoculum of 15x10(4) cells. This potent effect was confirmed by transmission electron microscopy where morphological deterioration of superficial structures mainly in the ventral disc, and formation of a great number of micro vesicles in the cytoplasm were found. We concluded that a direct chemical-oxidation attack by the active substances from Oleozon is one of the causes of the parasitocidal effect of this product. We suggest that the dose and cell density-dependent effect must be taken into account when prescription of this product for giardiasis treatment in humans.
authors.affiliation: Department of Biomedicine, Ozone Research Center from National Center for Scientific Research, P.O. Box 6412, Havana City 16000, Cuba. frank.hernandez@cnic.edu.cu
authors.forename: Frank
authors.initials: F
authors.lastname: Hernández
authors.forename: Damian
authors.initials: D
authors.lastname: Hernández
authors.forename: Zullyt
authors.initials: Z
authors.lastname: Zamora
authors.forename: Maritza
authors.initials: M
authors.lastname: Díaz
authors.forename: Odelsa
authors.initials: O
authors.lastname: Ancheta
authors.forename: Sandra
authors.initials: S
authors.lastname: Rodriguez
authors.forename: Dinorah
authors.initials: D
authors.lastname: Torres
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D016829:Giardia lamblia / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010084:Oxidation-Reduction; D010126:Ozone / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D000074242:Sunflower Oil
chemical_list: D010938:Plant Oils; D000074242:Sunflower Oil; D010126:Ozone