A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations.
This study establishes a filtration method for the safe removal of Bacillus anthracis spores which may contaminate DNA preparations. Centrifugal filtration with 0.1-microm filter units can be used following extraction of DNA from B. anthracis spores to render samples safe without compromising the sensitivity of diagnostic real-time PCR assays for B. anthracis.
Dauphin
Leslie A
LA
Division of Bioterrorism Preparedness and Response (DBPR), National Center for Preparedness, Detection, and Control of Infectious Diseases (NCPDCID), CDC, Atlanta, Georgia, USA. Ldauphin@CDC.GOV
Bowen
Michael D
MD
D000881:Anthrax / Q000175:diagnosis; D001408:Bacillus anthracis / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D023482:Bioterrorism; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000379:methods*; D005374:Filtration / Q000379:methods*; D050296:Microbial Viability; D008851:Micropore Filters; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial / Q000302:isolation & purification
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic