title: Adaptation of hybridomas to protein-free media results in a simplified two-step immunoglobulin M purification process.
abstract: An IgM antibody was purified from hybridoma supernatant containing serum using a three-step purification process comprising of tangential flow filtration, anion-exchange chromatography and size-exclusion chromatography. Recovery and purity were significantly improved upon adaptation of the hybridoma to serum-free media. The process could even be simplified by omitting the initial tangential flow filtration step. Even with a two-step purification process a purity of >98% and a recovery of >60% was obtained.
authors.affiliation: Bioprocessing Technology Institute, Agency for Science, Technology and Research, 20 Biopolis Way, No. 06-01 Centros, Singapore 138668, Singapore.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Lee
authors.forename: Anne
authors.initials: A
authors.lastname: Tscheliessnig
authors.forename: Allen
authors.initials: A
authors.lastname: Chen
authors.forename: Yih Yean
authors.initials: YY
authors.lastname: Lee
authors.forename: Gloria
authors.initials: G
authors.lastname: Adduci
authors.forename: Andre
authors.initials: A
authors.lastname: Choo
authors.forename: Alois
authors.initials: A
authors.lastname: Jungbauer
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D016895:Culture Media, Serum-Free; D006825:Hybridomas / Q000276:immunology*; D007075:Immunoglobulin M / Q000302:isolation & purification*; D051379:Mice
chemical_list: D003470:Culture Media; D016895:Culture Media, Serum-Free; D007075:Immunoglobulin M