title: The chiral separation of triazole pesticides enantiomers by amylose-tris (3,5-dimethylphenylcarbamate) chiral stationary phase.
abstract: The amylose-tris(3,5-dimethylphenylcarbamate) chiral stationary phase was synthesized and used to separate the enantiomers of triazole pesticides by high-performance liquid chromatography. The mobile phase was n-hexane-isopropanol applying a flow rate of 1.0 mL/min. Six triazole pesticides were enantioselectively separated. Myclobutanil, paclobutrazol, tebuconazole, and uniconazole obtained complete separation with the resolution factors of 5.73, 2.99, 1.72, and 2.07, respectively, and imazalil and diniconazole obtained partial separation with the resolution factors of 0.79 and 0.77 under the optimized conditions. The effect of the content of isopropanol as well as column temperature on the separation was investigated. A circular dichroism detector was used to identify the enantiomers and determine the elution orders. The results showed the low temperature was good for the chiral separation except for diniconazole. The thermodynamic parameters calculated based on linear Van't Hoff plots showed the chiral separations were controlled by enthalpy.
authors.affiliation: Department of Applied Chemistry, China Agricultural University, Beijing 100094, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Donghui
authors.initials: D
authors.lastname: Liu
authors.forename: Shuren
authors.initials: S
authors.lastname: Jiang
authors.forename: Yangguang
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000688:Amylose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007093:Imidazoles / Q000302:isolation & purification; D009570:Nitriles / Q000302:isolation & purification; D010575:Pesticides / Q000302:isolation & purification*; D048448:Phenylcarbamates; D013237:Stereoisomerism; D013696:Temperature; D013816:Thermodynamics; D014230:Triazoles / Q000302:isolation & purification*
chemical_list: D007093:Imidazoles; D009570:Nitriles; D010575:Pesticides; D048448:Phenylcarbamates; D014230:Triazoles; C080033:Chiralpak AD; C087114:tebuconazole; C017435:enilconazole; C053370:paclobutrazol; D000688:Amylose; C446685:systhane; C092705:uniconazole; C049346:diniconazole