Salt-stress induced alterations in protein profile and protease activity in the mangrove Bruguiera parviflora.
Two-month-old seedlings of Bruguiera parvifora were treated with varying levels of NaCl (100, 200 and 400 mM) under hydroponic culture. Total proteins were extracted from leaves of control and NaCl treated plants after 7, 14, 30 and 45 d of treatment and analysed by SDS-PAGE. As visualized from SDS-PAGE, the intensity of several protein bands of molecular weight 17, 23, 32, 33 and 34 kDa decreased as a result of NaCl treatment. The degree of decrease of these protein bands seemed to be roughly proportional to the external NaCl concentration. The most obvious change concerned a 23 kDa-polypeptide (SSP-23), which disappeared after 45 d treatment in 400 mM NaCl. Moreover, the SSP-23 protein, which disappeared in B. parviflora under salinity stress, reappeared when these salinized seedlings were desalinized. These observations suggest the possible involvement of these polypeptides for osmotic adjustment under salt stress. NaCl stress also caused an increase in the activity of both acid and alkaline protease. The increasing activity of proteases functions as a signal of salt stress in B. parviflora, which induces the reduction of protein level.
Parida
Asish Kumar
AK
National Institute for Plant Biodiversity Conservation and Research, Nayapalli, Bhubaneswar, Orissa, India. asishparida2003@yahoo.com
Das
Anath Bandhu
AB
Mittra
Bhabatosh
B
Mohanty
Prasanna
P
D016282:Aspartic Acid Endopeptidases / Q000187:drug effects / Q000378:metabolism; D001426:Bacterial Proteins / Q000187:drug effects / Q000378:metabolism; D010450:Endopeptidases / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D043982:Rhizophoraceae / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*
D001426:Bacterial Proteins; D010455:Peptides; D010940:Plant Proteins; D012965:Sodium Chloride; D010450:Endopeptidases; D010447:Peptide Hydrolases; D016282:Aspartic Acid Endopeptidases; C087822:alkaline protease