Enhanced production of poly (gamma-glutamic acid) from Bacillus licheniformis NCIM 2324 by using metabolic precursors.
The aim of the present work was to study the effect of addition of different amino acids and tricarboxylic acid cycle intermediates as metabolic precursors on the production of poly (gamma-glutamic acid) (PGA) by Bacillus licheniformis NCIM 2324. A maximum yield of 35.75 g/l was obtained with the medium supplemented with 0.5 mM L-glutamine and 10 mM alpha-ketoglutaric acid as compared to 26.12 g/l PGA achieved with the control in the absence of metabolic precursors. Addition of precursors also enhanced the utilization of L-glutamic acid during fermentation.
Bajaj
Ishwar B
IB
Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Mumbai, India. ishbajaj1@yahoo.co.in
Singhal
Rekha S
RS
D000596:Amino Acids / Q000494:pharmacology*; D001407:Bacillus / Q000187:drug effects* / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000494:pharmacology*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000378:metabolism
D000596:Amino Acids; D007656:Ketoglutaric Acids; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid