title: Establishing substantial equivalence: proteomics.
abstract: Wheat is a major crop in world agriculture and is consumed after processing into a range of food products. It is therefore of great importance to determine the consequences (intended and unintended) of transgenesis in wheat and whether genetically modified lines are substantially equivalent to those produced by conventional plant breeding. Proteomic analysis is one of several approaches which can be used to address these questions. Two-dimensional PAGE (2D PAGE) remains the most widely available method for proteomic analysis, but is notoriously difficult to reproduce between laboratories. We therefore describe methods which have been developed as standard operating procedures in our laboratory to ensure the reproducibility of proteomic analyses of wheat using 2D PAGE analysis of grain proteins.
authors.affiliation: Department of Plant Sciences, Rothamsted Research, Centre for Crop Genetic Improvement, Harpenden, Hertfordshire, UK.
authors.forename: Alison
authors.initials: A
authors.lastname: Lovegrove
authors.forename: Louise
authors.initials: L
authors.lastname: Salt
authors.forename: Peter R
authors.initials: PR
authors.lastname: Shewry
mesh_terms: D000478:Alkylation; D007525:Isoelectric Focusing; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D040901:Proteomics / Q000379:methods*; D012492:Salts / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D012492:Salts