title: MAP2-mediated in vitro interactions of brain microtubules and their modulation by cAMP.
abstract: Microtubule-associated proteins (MAPs) are involved in microtubule (MT) bundling and in crossbridges between MTs and other organelles. Previous studies have assigned the MT bundling function of MAPs to their MT-binding domain and its modulation by the projection domain. In the present work, we analyse the viscoelastic properties of MT suspensions in the presence or the absence of cAMP. The experimental data reveal the occurrence of interactions between MT polymers involving MAP2 and modulated by cAMP. Two distinct mechanisms of action of cAMP are identified, which involve on one hand the phosphorylation of MT proteins by the cAMP-dependent protein kinase A (PKA) bound to the end of the N-terminal projection of MAP2, and on the other hand the binding of cAMP to the RII subunit of the PKA affecting interactions between MTs in a phosphorylation-independent manner. These findings imply a role for the complex of PKA with the projection domain of MAP2 in MT-MT interactions and suggest that cAMP may influence directly the density and bundling of MT arrays in dendrites of neurons.
authors.affiliation: Department of Neurosciences, UMR 6187 CNRS, P.B.S., Poitiers University, 40 Avenue du, Recteur Pineau, 86022, Poitiers Cedex, France. jean.francois.leterrier@univ-poitiers.fr
authors.forename: J F
authors.initials: JF
authors.lastname: Leterrier
authors.forename: M
authors.initials: M
authors.lastname: Kurachi
authors.forename: T
authors.initials: T
authors.lastname: Tashiro
authors.forename: P A
authors.initials: PA
authors.lastname: Janmey
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004548:Elasticity; D004591:Electrophoresis, Polyacrylamide Gel; D008274:Magnesium / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D010766:Phosphorylation; D016548:Prosencephalon / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D013995:Time; D014404:Tubulin / Q000378:metabolism; D016875:tau Proteins / Q000378:metabolism
chemical_list: C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D014404:Tubulin; D016875:tau Proteins; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D008274:Magnesium