Development of porous collagen beads for chondrocyte culture.
A method for the preparation of bioresorbable collagen beads with an open porous structure is presented. These beads were prepared from collagen-alginate composite beads by removal of the alginate component. These collagen beads were suitable for rapid proliferation of chondrocytes in a dynamic, spinner culture system. Histology and immuno-histology showed that biochemical markers of chondrocytes are present during this cell proliferation, indicating that there was control of phenotype and that cell de-differentiation had not occurred. Unlike other 3-D scaffolds that have been used, the cells were amplified from very low cell densities and were able to proliferate freely without loss of phenotype.
Tebb
Tracy A
TA
CSIRO, Molecular and Health Technologies, Bayview Avenue, Clayton South, VIC, 3169, Australia.
Tsai
Shiao-Wen
SW
Glattauer
Veronica
V
White
Jacinta F
JF
Ramshaw
John A M
JA
Werkmeister
Jerome A
JA