Identification of tumor necrosis factor signaling-related proteins during Epstein-Barr virus-induced B cell transformation.
Epstein-Barr virus (EBV) infection in vitro transforms primary B cells into continuously proliferating lymphoblastoid cell lines (LCLs) that have been widely used as a genomic resource for variety of immunological and genetic studies. However, the biochemical and biological characteristics that distinguish LCLs from the B cells have not been thoroughly investigated. Our proteomic approach showed that EBV infection induced changes in the profiles of tumor necrosis factor (TNF) signaling-related proteins in LCLs including heat shock protein family members TNF receptor-associated protein 1 (TRAP-1), heat shock 70-kDa protein 9 (HSPA9)) and superoxide dismutase 2 (SOD2). In addition, our literature co-occurrence study placed TNF at the center of a gene cluster network of differentially expressed proteins in LCLs. This study suggested that deregulation of TNF signaling pathway could contribute to the cellular transformation and immortalization of the EBV-infected B cells.
Jeon
J-P
JP
Korea BioBank, Center for Genome Science, Korea National Institute of Health, Seoul, Korea.
Kim
J-W
JW
Park
B
B
Nam
H-Y
HY
Shim
S-M
SM
Lee
M-H
MH
Han
B-G
BG
D001402:B-Lymphocytes / Q000378:metabolism / Q000821:virology*; D002461:Cell Line, Transformed / Q000378:metabolism; D002472:Cell Transformation, Viral; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D011506:Proteins; D014409:Tumor Necrosis Factor-alpha