Monitoring of DNA damage in individuals exposed to petroleum hydrocarbons in Ecuador.
Currently, it is known that several chemical agents used or generated by the oil industry are classified as mutagens and/or carcinogens. Among these we have gasoline, diesel, butane gas, styrene, benzene, chloroform, and others. Studies have verified that these chemicals have effects in fertility (abortions, sterility); produce various upheavals, such as dizziness, nausea, muscular pain; and produce chromosomal damage at the DNA level, which in the long or medium run, can develop into cancer and leukemia. The genetic damage in exposed individuals was measured by means of the comet test, chromosomal alterations test, and the study of the CYP 1A1 and MSH2 genes. These methods were applied to determine the genotoxicity of hydrocarbons and their residue in human beings. When conducting these tests on the blood samples of individuals exposed to hydrocarbons (workers of oil companies) and of a control population of the area of study and Quito, it was found that, in effect, the exposed individuals presented a greater amount of damage at the DNA level as well as at the chromosomal level than the individuals from the control populations (P< 0.001). Thus, it can be determined that populations that are exposed to hydrocarbons are susceptible to developing genetic damage. Therefore, risk groups can be determined in certain zones where the oil impact has been greater.
Paz-y-Miño
César
C
Laboratorio de Genética Molecular y Citogenética Humana, Departamento de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Quito, Ecuador. cpazymino@uamericas.edu.ec
López-Cortés
Andrés
A
Arévalo
Melissa
M
Sánchez
María Eugenia
ME
D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure; D020552:Comet Assay; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D004484:Ecuador; D006801:Humans; D006838:Hydrocarbons / Q000009:adverse effects / Q000032:analysis*; D051718:MutS Homolog 2 Protein / Q000235:genetics; D009153:Mutagens; D009369:Neoplasms / Q000378:metabolism*; D016273:Occupational Exposure; D010578:Petroleum / Q000009:adverse effects / Q000032:analysis*; D011110:Polymorphism, Genetic
D006838:Hydrocarbons; D009153:Mutagens; D010578:Petroleum; D004247:DNA; D019363:Cytochrome P-450 CYP1A1; C497172:MSH2 protein, human; D051718:MutS Homolog 2 Protein