title: Clinical and experimental study of autologous fat grafting after processing by centrifugation and serum lavage.
abstract: BACKGROUND
  This clinical and experimental study compared adipose tissue transplant behavior after two different techniques of purifying: centrifugation at 3400 rpm for 3 min and serum lavage without centrifugation.
  
  
  METHODS
  Clinical evaluation was performed under standardized conditions for lipofilling on a series of 51 female patients, intentionally selected to have similar characteristics and assigned to two groups based on the method of processing. Experimentally, a culture system in diffusion chambers with vitaline membranes was designed to mimic the behavior and to study the morphology of the adipose tissue used for autografting. Survival, structure, and proliferation of the adipose cells in vitro were examined by classical histologic H&E staining and immunohistochemistry for leptin and cyclin D1.
  
  
  RESULTS
  The main differences encountered experimentally were the presence of a greater amount of preadipocytes in the noncentrifuged adipose tissue cultures and more distinctly expressed cell proliferation. The postoperative clinical results favored of the serum lavage purifying technique.
  
  
  CONCLUSIONS
  Our data suggest that with transplantation of noncentrifuged adipose tissue more active preadipocytes are applied which could possibly lead to better potential chances of survival and even de novo development of fat.
authors.affiliation: Unit of Plastic and Craniofacial Surgery, St. George University Hospital, 66 Peshtersko Sch., Plovdiv, 4000, Bulgaria. reginakhater@yahoo.com
authors.forename: Regina
authors.initials: R
authors.lastname: Khater
authors.forename: Pepa
authors.initials: P
authors.lastname: Atanassova
authors.forename: Yorii
authors.initials: Y
authors.lastname: Anastassov
authors.forename: Philippe
authors.initials: P
authors.lastname: Pellerin
authors.forename: Veronique
authors.initials: V
authors.lastname: Martinot-Duquennoy
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000637:transplantation; D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000293:Adolescent; D000328:Adult; D001707:Biopsy, Needle; D049109:Cell Proliferation; D002498:Centrifugation / Q000379:methods*; D015331:Cohort Studies; D004954:Esthetics; D005260:Female; D005500:Follow-Up Studies; D006084:Graft Rejection; D006085:Graft Survival; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011336:Probability; D018709:Statistics, Nonparametric; D007507:Therapeutic Irrigation / Q000379:methods*; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D055815:Young Adult