Expression and prognostic significance of the alpha B-crystallin gene in human hepatocellular carcinoma.
The aim of this study was to characterize expression of the alpha B-crystallin gene in human hepatocellular carcinomas, to investigate the relationship between expression of this gene and the prognosis of human hepatocellular carcinoma. Real-time polymerase chain reaction, reverse transcriptase-polymerase chain reaction and immunohistochemistry were used to characterize expression of the alpha B-crystallin gene in human hepatocellular carcinoma. Kaplan-Meier survival and Cox regression analyses were performed to evaluate the prognosis of human hepatocellular carcinoma. We characterized alpha B-crystallin gene expression in human hepatocellular carcinoma. Statistical analysis of hepatocellular carcinoma patients showed that patients expressing alpha B-crystallin have different survival rates relative to those not expressing this gene (P = .041). After 18 months, the survival rate of patients expressing alpha B-crystallin declined, but survival in the alpha B-crystallin-negative group remained stable. COX multi-factor analysis showed that alpha B-crystallin (P = .007) and venous invasion (P = .037) were independent prognosis factors for hepatocellular carcinoma. Expression of the alpha B-crystallin gene, which is related with the transferability and invasive capacity of hepatocellular carcinoma cells, can be used as a prognostic indicator in human hepatocellular carcinomas. It may also be involved in the malignant transformation of hepatocytes.
Tang
Qi
Q
Key Laboratory of Antibody Technique, Ministry of Health, Nanjing Medical University, Nanjing 210029, PR China. pippo3@vip.163.com
Liu
Yue-Fang
YF
Zhu
Xiao-Jing
XJ
Li
Yu-Hua
YH
Zhu
Jin
J
Zhang
Jian-Ping
JP
Feng
Zhen-Qing
ZQ
Guan
Xiao-Hong
XH
D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008113:Liver Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D016016:Proportional Hazards Models; D012334:RNA, Neoplasm / Q000032:analysis; D015996:Survival Rate; D038203:alpha-Crystallin B Chain / Q000235:genetics* / Q000378:metabolism
D014408:Biomarkers, Tumor; D012334:RNA, Neoplasm; D038203:alpha-Crystallin B Chain