title: Cymantrene conjugation modulates the intracellular distribution and induces high cytotoxicity of a cell-penetrating peptide.
abstract: The conjugation of cymantrene CpMn(CO)(3) to cell-penetrating peptide hCT(18-32)-k7 alters the intracellular distribution in MCF-7 cells compared to the unmodified peptide, as visualized by fluorescence microscopy, and leads to an increased nuclear accumulation; the peptide and cymantrene compound themselves are not toxic, but the bioconjugate shows a significant cytotoxicity with an IC(50) value of 36 micromol l(-1).
authors.affiliation: Institut für Biochemie, Universität Leipzig, Brüderstr. 34, D-04103, Leipzig, Germany. ines.neundorf@uni-leipzig.de
authors.forename: Ines
authors.initials: I
authors.lastname: Neundorf
authors.forename: Jan
authors.initials: J
authors.lastname: Hoyer
authors.forename: Katrin
authors.initials: K
authors.lastname: Splith
authors.forename: Robert
authors.initials: R
authors.lastname: Rennert
authors.forename: Harmel W
authors.initials: HW
authors.lastname: Peindy N'dongo
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schatzschneider
mesh_terms: D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity*
chemical_list: D009942:Organometallic Compounds; D010455:Peptides; C044679:manganese cyclopentadienyl tricarbonyl