CDC25 protein expression and interaction with DAZL in human corpus luteum.
OBJECTIVE
To determine whether the human corpus luteum exhibits CDC25 protein expression and its expression pattern, and identify the interaction with DAZL (deleted in azoospermia-like) in human luteal cells.


METHODS
Experimental study.


METHODS
Medical research laboratory in a university hospital.


METHODS
Twelve human corpus luteum (CL), four in the early stage, four in the midstage, and four in the late stage of the luteal phase, respectively, and 10 granulosa-lutein cells from IVF patients.


METHODS
Immunohistochemical stain and Western blot were used to characterize the expression of CDC25 protein, and protein immunoprecipitation was used to identify the interaction of CDC25 with DAZL in human luteal cells.


METHODS
CDC25 protein expression pattern in different stages of luteal phase and interaction with DAZL.


RESULTS
In this study, we show evidence that CDC25 protein is express in granulosa-lutein cells from different stages of CL, and identified only the CDC25A interaction with DAZL in luteal cells.


CONCLUSIONS
The CL is the final form of a developing follicle and is the major endocrine component of the ovary in maintaining early successful pregnancy. Expression of CDC25 protein throughout different stages of the ovarian cycles implies important functional roles in the regulation of female reproduction.
Pan
Hsien-An
HA
Department of Obstetrics and Gynecology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
Lee
Yeu-Chin
YC
Teng
Yen-Ni
YN
Tsai
Shaw-Jeng
SJ
Lin
Yung-Ming
YM
Kuo
Pao-Lin
PL
D015153:Blotting, Western; D003338:Corpus Luteum / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007150:Immunohistochemistry; D008183:Luteal Phase / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D016601:RNA-Binding Proteins / Q000378:metabolism*; D020687:cdc25 Phosphatases / Q000378:metabolism*
C101358:DAZL protein, human; D016601:RNA-Binding Proteins; C517004:CDC25A protein, human; C517008:CDC25B protein, human; C517009:CDC25C protein, human; D020687:cdc25 Phosphatases