Electron-transfer subunits of the NiFe hydrogenases in Thiocapsa roseopersicina BBS.
Thiocapsa roseopersicina BBS contains at least three different active NiFe hydrogenases: two membrane-bound enzymes and one apparently localized in the cytoplasm. In addition to the small and large structural subunits, additional proteins are usually associated with the NiFe hydrogenases, connecting their activity to other redox processes in the cells. The operon of the membrane-associated hydrogenase, HynSL, has an unusual gene arrangement: between the genes coding for the large and small subunits, there are two open reading frames, namely isp1 and isp2. Isp1 is a b-type haem-containing transmembrane protein, whereas Isp2 displays marked sequence similarity to the heterodisulfide reductases. The other membrane-bound (Hup) NiFe hydrogenase contains the hupC gene, which codes for a cytochrome b-type protein that probably plays a role in electron transport. The operon of the NAD(+)-reducing Hox hydrogenase contains a hoxE gene. In addition to the hydrogenase and diaphorase parts of the complex, the fifth HoxE subunit may serve as a third redox gate of this enzyme. The physiological functions of these putative electron-mediating subunits were studied by disruption of their genes. The deletion of some accessory proteins dramatically reduced the in vivo activities of the hydrogenases, although they were fully active in vitro. The absence of HupC resulted in a decrease in HupSL activity in the membrane, but removal of the Isp1 and Isp2 proteins did not have any significant effect on the location of HynSL activity. Through the use of a tagged HoxE protein, the whole Hox hydrogenase pentamer could be purified as an intact complex.
Palágyi-Mészáros
Lívia S
LS
Department of Biotechnology, University of Szeged, Hungary.
Maróti
Judit
J
Latinovics
Dóra
D
Balogh
Tímea
T
Klement
Eva
E
Medzihradszky
Katalin F
KF
Rákhely
Gábor
G
Kovács
Kornél L
KL
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D002462:Cell Membrane / Q000201:enzymology; D004579:Electron Transport; D006859:Hydrogen / Q000378:metabolism; D006864:Hydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007438:Introns; D007700:Kinetics; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016366:Open Reading Frames; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D020616:Thiocapsa roseopersicina / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D008565:Membrane Proteins; D021122:Protein Subunits; D006859:Hydrogen; C043296:nickel-iron hydrogenase; D006864:Hydrogenase