title: Drawing blood from rats through the saphenous vein and by cardiac puncture.
abstract: Drawing blood from rodents is necessary for a large number of both in vitro and in vivo studies. Sites of blood draws are numerous in rodents: retro-orbital sinus, jugular vein, maxillary vein, saphenous vein, heart. Each technique has its advantages and disadvantages, and some are not approved any more in some countries (e.g., retro-orbital draws in Holland). A discussion of different techniques for drawing blood are available (1-3). Here, we present two techniques for drawing blood from rats, each with its specific applications. Blood draw from the saphenous vein, provided it is done properly, induces minimal distress in animals and does not require anesthesia. This technique allows repeated draws of small amounts of blood, such as needed for pharmacokinetic studies (4,5), determining plasma chemistry, or blood counts (6). Cardiac puncture allows the collection of large amounts of blood from a single animal (up to 10 ml of blood can be drawn from a 150 g rat). This technique is therefore very useful as a terminal procedure when drawing blood from the saphenous would not provide a large enough sample. We use cardiac puncture when we need sufficient amounts of serum from a specific strain of rats to grow T lymphocyte lines in vitro (4-9).
authors.affiliation: Department of Physiology and Biophysics, University of California, Irvine, CA, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Beeton
authors.forename: Adriana
authors.initials: A
authors.lastname: Garcia
authors.forename: K George
authors.initials: KG
authors.lastname: Chandy
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods*; D006321:Heart; D011677:Punctures; D051381:Rats; D012501:Saphenous Vein