A cytoplasmic variant of the KH-type splicing regulatory protein serves as a decay-promoting factor for phosphoglycerate kinase 2 mRNA in murine male germ cells.
Phosphoglycerate kinase 2 (PGK2) is a germ cell-specific protein whose mRNA is translationally regulated in the mammalian testis. Using RNA affinity chromatography with the 3'-untranslated region (UTR) of Pgk2 mRNA and adult testis extracts, several associated proteins including a novel isoform of the AU-rich element RNA-binding protein and KH-type splicing regulatory protein (KSRP) were identified. KSRP, a protein of approximately 75 kDa, is widely expressed in somatic and germ cells where it is primarily nuclear. In addition to the approximately 75-kDa KSRP, a approximately 52-kD KSRP, t-KSRP, is present in the cytoplasm of a subpopulation of germ cells. t-KSRP binds directly to a 93-nt sequence (designated the F1 region) of the 3'-UTR of the Pgk2 mRNA and destabilizes Pgk2 mRNA constructs in testis extracts and in transfected cells. We conclude that this testicular variant of the multifunctional nucleic acid-binding protein, KSRP, serves as a decay-promoting factor for Pgk2 mRNA in male germ cells.
Xu
Mingang
M
Center for Research on Reproduction and Women's Health, University of Pennsylvania School of Medicine, Philadelphia, PA 6080, USA.
McCarrey
John R
JR
Hecht
Norman B
NB
D020413:3' Untranslated Regions / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D003593:Cytoplasm / Q000378:metabolism; D014644:Genetic Variation; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008540:Meiosis; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010735:Phosphoglycerate Kinase / Q000235:genetics* / Q000378:metabolism; D038941:Polypyrimidine Tract-Binding Protein / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D013737:Testis / Q000201:enzymology / Q000378:metabolism*; D015534:Trans-Activators / Q000302:isolation & purification / Q000378:metabolism*
D020413:3' Untranslated Regions; D007527:Isoenzymes; C507913:Khsrp protein, mouse; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; C429773:Ptbp2 protein, mouse; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D015534:Trans-Activators; D038941:Polypyrimidine Tract-Binding Protein; D010735:Phosphoglycerate Kinase; C104317:phosphoglycerate kinase, testis specific