High-performance liquid chromatography/electrospray mass spectrometry analysis of the mycotoxin aurofusarin.
High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) can be used for simultaneous quantification of various mycotoxins in contaminated food samples. Therefore, multi-mycotoxin methods have been developed in the last couple of years. To enlarge these methods for further analytes, we have developed a LC-MS/MS method for the quantification of the mycotoxin aurofusarin. Additionally, further LC- MS(n) experiments were performed to demonstrate the fragmentation pattern of aurofusarin. Applicable multiple reaction monitoring (MRM) transitions of aurofusarin were found and optimized by parameter variation of the tandem mass spectrometer. The applicability of the developed method was tested by analysis of naturally contaminated wheat.
Neuhof
Torsten
T
Köppen
Robert
R
Koch
Matthias
M
Nehls
Irene
I
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis*; D009285:Naphthoquinones / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014908:Triticum / Q000737:chemistry
D009183:Mycotoxins; D009285:Naphthoquinones; C083578:aurofusarin