Structures and reaction pathways of the molybdenum centres of sulfite-oxidizing enzymes by pulsed EPR spectroscopy.
SOEs (sulfite-oxidizing enzymes) are physiologically vital and occur in all forms of life. During the catalytic cycle, the five-co-ordinate square pyramidal oxo-molybdenum active site passes through the Mo(V) state, and intimate details of the structure can be obtained from variable frequency pulsed EPR spectroscopy through the hyperfine and nuclear quadrupole interactions of nearby magnetic nuclei. By employing variable spectrometer operational frequencies, it is possible to optimize the measurement conditions for difficult quadrupolar nuclei of interest (e.g. (17)O, (33)S, (35)Cl and (37)Cl) and to simplify the interpretation of the spectra. Isotopically labelled model Mo(V) compounds provide further insight into the electronic and geometric structures and chemical reactions of the enzymes. Recently, blocked forms of SOEs having co-ordinated sulfate, the reaction product, were detected using (33)S (I=3/2) labelling. This blocking of product release is a possible contributor to fatal human sulfite oxidase deficiency in young children.
Enemark
John H
JH
Department of Chemistry, University of Arizona, 1306 E. University Blvd, Tucson, AZ 85721-0041, USA. jenemark@u.arizona.edu
Astashkin
Andrei V
AV
Raitsimring
Arnold M
AM
D002384:Catalysis; D002712:Chlorides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling; D008024:Ligands; D008982:Molybdenum / Q000378:metabolism*; D010103:Oxygen Isotopes; D050876:Sulfite Oxidase / Q000737:chemistry* / Q000378:metabolism*; D013460:Sulfur Isotopes
D002712:Chlorides; D008024:Ligands; D010103:Oxygen Isotopes; D013460:Sulfur Isotopes; D008982:Molybdenum; D050876:Sulfite Oxidase