title: Kekkon5 is an extracellular regulator of BMP signaling.
abstract: Precise spatial and temporal control of Drosophila Bone Morphogenetic Protein (BMP) signaling is achieved by a host of extracellular factors that modulate ligand distribution and activity. Here we describe Kekkon5 (Kek5), a transmembrane protein containing leucine-rich repeats (LRRs), as a novel regulator of BMP signaling in Drosophila. We find that loss or gain of kek5 disrupts crossvein development and alters the early profile of phosphorylated Mad and dSRF in presumptive crossvein cells. kek5 phenotypic effects closely mimic those observed with Short gastrulation (Sog), but do not completely recapitulate the effects of dominant negative BMP receptors. We further demonstrate that Kek5 is able to antagonize the BMP ligand Glass bottom boat (Gbb) and that the Kek5 LRRs are required for BMP inhibitory activity, while the Ig domain is dispensable in this context. Our identification of Kek5 as a modulator of BMP signaling supports the emerging notion that LIG proteins function as diverse regulators of cellular communication.
authors.affiliation: Department of Biology, Indiana University, Jordan Hall, 1001 E 3d Street., Bloomington, IN 47405, USA.
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Evans
authors.forename: Harita
authors.initials: H
authors.lastname: Haridas
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Duffy
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000502:physiology*; D002450:Cell Communication; D002490:Central Nervous System / Q000196:embryology / Q000254:growth & development; D004268:DNA-Binding Proteins / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D010766:Phosphorylation; D011679:Pupa; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000378:metabolism; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D019485:Bone Morphogenetic Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C539249:Kek5 protein, Drosophila; C112506:MAD protein, Drosophila; D008565:Membrane Proteins; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; C089440:gbb protein, Drosophila; C091428:sog protein, Drosophila