title: N2-ethyldeoxyguanosine as a potential biomarker for assessing effects of alcohol consumption on DNA.
abstract: Head and neck cancers are causally related to alcohol consumption, but the underlying mechanisms are unclear. Ethanol is metabolized to acetaldehyde, an experimental carcinogen. Quantitation of the major DNA adduct of acetaldehyde, N2-ethylidenedeoxyguanosine, in human tissues could help to elucidate the mechanism of alcohol carcinogenicity. We applied a quantitative method for the analysis of this adduct, measured as the NaBH3CN reduction product N2-ethyldeoxyguanosine (N2-ethyl-dGuo) by liquid chromatography-electrospray ionization-tandem mass spectrometry-selected reaction monitoring, on DNA (0.04 +/- 0.03 mg) isolated from blood collected from control subjects recruited from two studies conducted in different areas of Europe between 1999 and 2005. The group selected from the first study (n = 127) included alcohol drinkers and abstainers while the group from the second study (n = 50) included only heavy drinkers. N2-ethyl-dGuo was detected in all DNA samples. After adjusting for potential confounders, in the first study, drinkers showed a higher level of N2-ethyl-dGuo (5,270 +/- 8,770 fmol/micromol dGuo) compared with nondrinkers (2,690 +/- 3040 fmol/micromol dGuo; P = 0.04). A significant trend according to dose was observed in both studies (P = 0.02 and 0.04, respectively). Taking into account the amount of alcohol consumption, adduct levels were higher in younger compared with older subjects (P = 0.01), whereas no differences were observed comparing men with women. These results show the feasibility of quantifying N2-ethyl-dGuo in small-volume blood samples and are consistent with the hypothesis that ethanol contributes to carcinogenesis through DNA adducts formation.
authors.affiliation: IARC, cours Albert Thomas 150, 69008, Lyon, France.
authors.forename: Silvia
authors.initials: S
authors.lastname: Balbo
authors.forename: Mia
authors.initials: M
authors.lastname: Hashibe
authors.forename: Sarolta
authors.initials: S
authors.lastname: Gundy
authors.forename: Paul
authors.initials: P
authors.lastname: Brennan
authors.forename: Cristina
authors.initials: C
authors.lastname: Canova
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Simonato
authors.forename: Franco
authors.initials: F
authors.lastname: Merletti
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Richiardi
authors.forename: Antonio
authors.initials: A
authors.lastname: Agudo
authors.forename: Xavier
authors.initials: X
authors.lastname: Castellsagué
authors.forename: Ariana
authors.initials: A
authors.lastname: Znaor
authors.forename: Renato
authors.initials: R
authors.lastname: Talamini
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bencko
authors.forename: Ivana
authors.initials: I
authors.lastname: Holcátová
authors.forename: Mingyao
authors.initials: M
authors.lastname: Wang
authors.forename: Stephen S
authors.initials: SS
authors.lastname: Hecht
authors.forename: Paolo
authors.initials: P
authors.lastname: Boffetta
mesh_terms: D000328:Adult; D000428:Alcohol Drinking; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D012907:Smoking / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D015415:Biomarkers; D018736:DNA Adducts; C492934:N2-ethyl-2'-deoxyguanosine; D003849:Deoxyguanosine