Synthesis and biological evaluation of a 2-aryl polyhydroxylated pyrrolidine alkaloid-based library.
Inspired by polyhydroxylated pyrrolidine alkaloid natural products, a 18-membered library of 2-aryl polyhydroxylated pyrrolidines has been efficiently prepared in two or three synthetic steps from the known chiral cyclic nitrones with high yield and purity and excellent stereoselectivity. The inhibitory activity of all these compounds against various glycosidase enzymes was evaluated. Interestingly, 15 and 19 show better inhibitory activities than radicamine A (20) and B (18) against alpha-glucosidases. The IC(50) values of 15 and 19 are 1.1 and 0.5 microM, respectively. In this study, we also discovered the substituent(s) on the aryl ring could affect the inhibition potency and selectivity against glycosidases.
Tsou
En-Lun
EL
The Genomics Research Center, Academia Sinica, No. 128, Academia Road Sec. 2, Nankang District, Taipei 11529, Taiwan.
Chen
Sih-Yu
SY
Yang
Ming-Hsun
MH
Wang
Shih-Chi
SC
Cheng
Ting-Ren Rachel
TR
Cheng
Wei-Chieh
WC
D000470:Alkaloids / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D015195:Drug Design; D065089:Glycoside Hydrolase Inhibitors; D020128:Inhibitory Concentration 50; D011759:Pyrrolidines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D013237:Stereoisomerism; D000520:alpha-Glucosidases / Q000378:metabolism; D044902:beta-Mannosidase / Q000037:antagonists & inhibitors / Q000378:metabolism
D000470:Alkaloids; D065089:Glycoside Hydrolase Inhibitors; D011759:Pyrrolidines; D000520:alpha-Glucosidases; D044902:beta-Mannosidase