title: [Studies on chemical constituents and antibacterial activity from n-butanol extract of Sarcandra glabra].
abstract: OBJECTIVE
  To study the chemical constituents and the antibacterial activity from n-butanol extract of Sarcandra glabra.
  
  
  METHODS
  The compounds were isolated by Diaion HP-20, Sephadex LH-20, MCI CHP-20 and silica gel column chromatographic methods. Their structures were identified on the basis of physicochemical and spectroscopic analysis. The antibacterial effect of the compounds were measured against Staphylococcus aureus by filterpaper slice method, finally the antibacterial ring in each group was recorded after 24 hours.
  
  
  RESULTS
  Seven constituents were isolated and elucidated as 5, 7, 3', 4'-tetrahydroxy-6-C-beta-D-glucopyranosylflavanone (1), kaempferol-3-O-beta-D-glucuronide (2), fraxidin (3), isofraxidin (4), isofraxidin-7-O-beta-D-glucopyranoside (5), kaempferol (6), pinostrobin (7). Diameters (in mm) of antibacterial ring in the compounds 2, 5, 6 were orderly recorded as follows: 14.67 +/- 0.08, 11.14 +/- 1.06, 8.26 +/- 1.26 and the compound 4 is not effective.
  
  
  CONCLUSIONS
  Compounds 1-3 and 5 were isolated from S. glabra for the first time.
authors.affiliation: Research and Development Center of Natural Medicine, Zhejiang Forestry University, Linan 311300, China. yuan_ke001@163.com
authors.forename: Ke
authors.initials: K
authors.lastname: Yuan
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Zhu
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Si
authors.forename: Hai-ke
authors.initials: HK
authors.lastname: Cai
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Ding
authors.forename: Yuan-jiang
authors.initials: YJ
authors.lastname: Pan
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000440:Butanols / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000440:Butanols; D004365:Drugs, Chinese Herbal