Post-reassortment amino acid change in the hemagglutinin of a human-avian influenza H5N1 reassortant virus alters its antigenic specificity.
It was shown earlier that the reassortant influenza virus having hemagglutinin (HA) gene of A/Duck/Primorie/2621/2001 (H5N2) virus and 7 genes of A/Puerto Rico/8/34 (H1N1) virus produced low yields in embryonated chicken eggs. We found that a variant reassortant selected by serial passages in eggs produced higher yields than the initial reassortant. The variant reassortant had an amino acid substitution in the hemagglutinin N244D (H3 numbering). In this report we demonstrated that the post-reassortment amino acid substitution N244D altered the antigenic specificity of HA as revealed by the loss of reactivity with an anti-H5 monoclonal antibody in hemagglutination-inhibition (HI) test. The results are discussed in association with the evolution of H5 hemagglutinin.
Rudneva
I A
IA
D.I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, Moscow, Russia.
Timofeeva
T A
TA
Ilyushina
N A
NA
Varich
N L
NL
Kochergin-Nikitsky
K S
KS
Kaverin
N V
NV
D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002642:Chick Embryo; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics*; D053125:Influenza A Virus, H5N2 Subtype / Q000235:genetics*; D016865:Reassortant Viruses / Q000235:genetics* / Q000276:immunology; D012692:Serial Passage; D014776:Virus Cultivation
D000911:Antibodies, Monoclonal; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus