Age-related differences in the dose-response relationship of muscle protein synthesis to resistance exercise in young and old men.
We investigated how myofibrillar protein synthesis (MPS) and muscle anabolic signalling were affected by resistance exercise at 20-90% of 1 repetition maximum (1 RM) in two groups (25 each) of post-absorptive, healthy, young (24 +/- 6 years) and old (70 +/- 5 years) men with identical body mass indices (24 +/- 2 kg m(-2)). We hypothesized that, in response to exercise, anabolic signalling molecule phosphorylation and MPS would be modified in a dose-dependant fashion, but to a lesser extent in older men. Vastus lateralis muscle was sampled before, immediately after, and 1, 2 and 4 h post-exercise. MPS was measured by incorporation of [1,2-(13)C] leucine (gas chromatography-combustion-mass spectrometry using plasma [1,2-(13)C]alpha-ketoisocaparoate as surrogate precursor); the phosphorylation of p70 ribosomal S6 kinase (p70s6K) and eukaryotic initiation factor 4E binding protein 1 (4EBP1) was measured using Western analysis with anti-phosphoantibodies. In each group, there was a sigmoidal dose-response relationship between MPS at 1-2 h post-exercise and exercise intensity, which was blunted (P < 0.05) in the older men. At all intensities, MPS fell in both groups to near-basal values by 2-4 h post-exercise. The phosphorylation of p70s6K and 4EBP1 at 60-90% 1 RM was blunted in older men. At 1 h post-exercise at 60-90% 1 RM, p70s6K phosphorylation predicted the rate of MPS at 1-2 h post-exercise in the young but not in the old. The results suggest that in the post-absorptive state: (i) MPS is dose dependant on intensity rising to a plateau at 60-90% 1 RM; (ii) older men show anabolic resistance of signalling and MPS to resistance exercise.
Kumar
Vinod
V
University of Nottingham, School of Graduate Entry Medicine and Health, City Hospital, Uttoxeter Road, Derby, DE22 3DT, UK.
Selby
Anna
A
Rankin
Debbie
D
Patel
Rekha
R
Atherton
Philip
P
Hildebrandt
Wulf
W
Williams
John
J
Smith
Kenneth
K
Seynnes
Olivier
O
Hiscock
Natalie
N
Rennie
Michael J
MJ
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000328:Adult; D000368:Aged; D000375:Aging / Q000473:pathology / Q000502:physiology*; D018797:Cell Cycle Proteins; D015444:Exercise / Q000502:physiology*; D006801:Humans; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000145:classification / Q000166:cytology / Q000378:metabolism; D009124:Muscle Proteins / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000502:physiology; D009210:Myofibrils / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D055070:Resistance Training; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D015398:Signal Transduction; D055815:Young Adult
D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; C087000:EIF4EBP1 protein, human; D009124:Muscle Proteins; D010750:Phosphoproteins; D038762:Ribosomal Protein S6 Kinases, 70-kDa