Double apheresis of peripheral blood stem cells in a single day in children mobilized by granulocyte colony-stimulating factor for transplantation.
Due to the movement of hematopoietic stem cells through the bone marrow environment, it may be possible to effectively harvest peripheral blood stem cells in a second apheresis within a few hours after a first apheresis. In a retrospective analysis, 107 aphereses were performed in consecutive 33 pediatric and six healthy pediatric donors who received granulocyte-colony stimulating factor at 10 microg/kg/day or 400 microg/m(2)/day for five days. The median age and weight of cases were seven yr (range 1-19) and 20 kg (range 8-87). The toxicities related to apheresis procedure were minimal in both aphereses. In 22 double aphereses, the average number of CD34-positive cells per body weight (kg) collected was 5.3 +/- 4.2 (range 0.6-16.6) and 4.7 +/- 3.1 (range 0.2-10.9) x 10(6) in the first and second aphereses, respectively (p = 0.569). Multivariate analysis showed that number of CD34-positive cells collected in the first apheresis (p = 0.008) was an independent factor of increased CD34-positive cells in the second apheresis. Double apheresis in a single day was feasible and this procedure may be able to lessen the burden of apheresis compared to two consecutive-day apheresis.
Okamoto
Yasuhiro
Y
Department of Pediatrics, University of Tokushima, Tokushima, Japan. okamoto@m2.kufm.kagoshima-u.ac.jp
Watanabe
Tsutomu
T
Watanabe
Hiroyoshi
H
Onishi
Toshihiro
T
Kawano
Yoshifumi
Y
D000293:Adolescent; D018952:Antigens, CD34; D001781:Blood Component Removal / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D003115:Colony-Stimulating Factors / Q000627:therapeutic use*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D007223:Infant; D008297:Male; D009369:Neoplasms / Q000628:therapy*; D012189:Retrospective Studies; D013601:T-Lymphocytes; D013997:Time Factors; D055815:Young Adult
D018952:Antigens, CD34; D003115:Colony-Stimulating Factors; D016179:Granulocyte Colony-Stimulating Factor