Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai.
The marine mollusk Aplysia is a useful model organism for studying the cellular bases of behavior and plasticity. However, molecular studies of Aplysia have been limited by the lack of genomic information. Recently, a large scale characterization of neuronal transcripts was performed in A. californica. Here, we report the analysis of a parallel set of neuronal transcripts from a closely related species A. kurodai found in the northwestern Pacific. We collected 4,859 nonredundant sequences from the nervous system tissue of A. kurodai. By performing microarray and real-time PCR analyses, we found that ApC/EBP, matrilin, antistasin, and eIF3e clones were significantly up-regulated and a BAT1 homologous clone was significantly down-regulated by 5-HT treatment. Among these, we further demonstrated that the Ap-eIF3e plays a key role in 5-HT-induced long-term facilitation (LTF) as a positive regulator.
Lee
Yong-Seok
YS
National Creative Research Initiative, Center for Memory and Institute of Molecular Biology and Genetics, Department of Biological Sciences, College of Natural Sciences, Seoul National University, San 56-1 Silim-dong, Gwanak-gu, Seoul 151-747, Korea.
Choi
Sun-Lim
SL
Kim
Tae-Hyung
TH
Lee
Jin-A
JA
Kim
Hyong Kyu
HK
Kim
Hyoung
H
Jang
Deok-Jin
DJ
Lee
Jennifer J
JJ
Lee
Sunghoon
S
Sin
Gwang Sik
GS
Kim
Chang-Bae
CB
Suzuki
Yutaka
Y
Sugano
Sumio
S
Kubo
Tai
T
Moroz
Leonid L
LL
Kandel
Eric R
ER
Bhak
Jong
J
Kaang
Bong-Kiun
BK
D000818:Animals; D001048:Aplysia / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D017931:DNA Primers; D020224:Expressed Sequence Tags; D017774:Long-Term Potentiation / Q000187:drug effects; D009473:Neuronal Plasticity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D012701:Serotonin / Q000494:pharmacology
D017931:DNA Primers; D012333:RNA, Messenger; D012701:Serotonin