title: Modulation of human neutrophil oxidative metabolism and degranulation by extract of Tamarindus indica L. fruit pulp.
abstract: The tamarind (Tamarindus indica L.) is indigenous to Asian countries and widely cultivated in the American continents. The tamarind fruit pulp extract (ExT), traditionally used in spices, food components and juices, is rich in polyphenols that have demonstrated anti-atherosclerotic, antioxidant and immunomodulatory activities. This study evaluated the modulator effect of a crude hydroalcoholic ExT on some peripheral human neutrophil functions. The neutrophil reactive oxygen species generation, triggered by opsonized zymosan (OZ), n-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA), and assessed by luminol- and lucigenin-enhanced chemiluminescence (LumCL and LucCL, respectively), was inhibited by ExT in a concentration-dependent manner. ExT was a more effective inhibitor of the PMA-stimulated neutrophil function [IC50 (in microg/10(6)cells)=115.7+/-9.7 (LumCL) and 174.5+/-25.9 (LucCL)], than the OZ- [IC50=248.5+/-23.1 (LumCL) and 324.1+/-34.6 (LucCL)] or fMLP-stimulated cells [IC50=178.5+/-12.2 (LumCL)]. The ExT also inhibited neutrophil NADPH oxidase activity (evaluated by O2 consumption), degranulation and elastase activity (evaluated by spectrophotometric methods) at concentrations higher than 200 microg/10(6)cells, without being toxic to the cells, under the conditions assessed. Together, these results indicate the potential of ExT as a source of compounds that can modulate the neutrophil-mediated inflammatory diseases.
authors.affiliation: Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. Avenida Bandeirantes n 3900, CEP 14049-900, Ribeirão Preto, SP, Brazil.
authors.forename: Fabiana S
authors.initials: FS
authors.lastname: Paula
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Kabeya
authors.forename: Alexandre
authors.initials: A
authors.lastname: Kanashiro
authors.forename: Andréa S G
authors.initials: AS
authors.lastname: de Figueiredo
authors.forename: Ana Elisa C S
authors.initials: AE
authors.lastname: Azzolini
authors.forename: Sérgio A
authors.initials: SA
authors.lastname: Uyemura
authors.forename: Yara Maria
authors.initials: YM
authors.lastname: Lucisano-Valim
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D010703:Phorbol Esters; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D029861:Tamarindus / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010703:Phorbol Esters; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C008874:phorbol-12,13-diacetate; D019255:NADPH Oxidases; D010196:Pancreatic Elastase