Genetic immunization with the immunodominant antigen P48 of Mycoplasma agalactiae stimulates a mixed adaptive immune response in BALBc mice.
A DNA vaccine against contagious agalactia was developed for the first time, encoding the P48 of Mycoplasma agalactiae. Specific immune responses elicited in BALB/c mice were evaluated. Both total IgG and IgG1 were detected in mice vaccinated with pVAX1/P48. Proliferation of mononuclear cells of the spleen, levels of gamma interferon, interleukin-12, and interleukin-2 mRNAs were enhanced in immunized animals. Results indicate that pVAX1/P48 vaccination induced both T(h)1 and T(h)2 immune responses. Nucleic acid immunization could be a new strategy against M. agalactiae infections and may be potentially used to develop vaccines for other Mycoplasma diseases.
Chessa
Bernardo
B
Sezione di Malattie Infettive del Dipartimento di Patologia Speciale e Clinica Veterinaria, Università degli Studi di Sassari, Via Vienna 2, 07100 Sassari, Italy.
Pittau
Marco
M
Puricelli
Maria
M
Zobba
Rosanna
R
Coradduzza
Elisabetta
E
Dall'ara
Paola
P
Rosati
Sergio
S
Poli
Giorgio
G
Alberti
Alberto
A
D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000627:therapeutic use; D002460:Cell Line; D016207:Cytokines / Q000235:genetics; D005260:Female; D006801:Humans; D007110:Immunity, Active; D007074:Immunoglobulin G / Q000097:blood; D050834:Interferon-Stimulated Gene Factor 3, gamma Subunit / Q000276:immunology*; D007668:Kidney / Q000196:embryology; D051379:Mice; D008807:Mice, Inbred BALB C; D009175:Mycoplasma Infections / Q000276:immunology*; D045644:Mycoplasma agalactiae / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D012418:Ruminants; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014162:Transfection; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000627:therapeutic use*
D001428:Bacterial Vaccines; D016207:Cytokines; D007074:Immunoglobulin G; D050834:Interferon-Stimulated Gene Factor 3, gamma Subunit; C494228:Isgf3g protein, mouse; D012333:RNA, Messenger; D019444:Vaccines, DNA