[Effect of mycophenolate mofetil on the expression of early inflammatory reaction in diabetic rats].
OBJECTIVE
To investigate the effect of mycophenolate mofetil(MMF) on early inflammatory reaction of renal lesion in streptozotocin(STZ)-induced diabetic rats.


METHODS
Thirty-six male Sprague-Dawley rats were randomly divided into 3 groups after uninephrectomy: normal control group, diabetic model group, and MMF-treated group. Six rats in each group were sacrificed at the 4th week and 14th week after STZ injection. Twenty-four hour urinary protein (24 h Upro) count was measured before death. The expressions of regulated on activation of normal T expressed and secreted (RANTES),ectodermal dysplasia (ED-1)and Col-IV protein in the renal tissue were detected by immunohistochemistry. The expression of RANTES mRNA in the renal tissue was detected by RT-PCR.


RESULTS
MMF prevented the increasing of 24h Upro in diabetic rats,and the expressions of RANTES,ED-1,Col-IV protein and RANTES mRNA in the kidney of MMF-treated rats were significantly decreased.


CONCLUSIONS
MMF plays an early renal protective role in diabetic nephropathy, possibly through inhibition of early inflammatory reaction.
Peng
Lin
L
Department of Nephrology, Third Xiangya Hospital,Central South University, Changsha 410013, China.
Zhang
Hao
H
Xu
Guo
G
Tang
Ren-hong
RH
D000818:Animals; D018946:Chemokine CCL5 / Q000096:biosynthesis* / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000378:metabolism / Q000517:prevention & control*; D053331:Ectodysplasins / Q000096:biosynthesis* / Q000235:genetics; D007249:Inflammation / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008297:Male; D009173:Mycophenolic Acid / Q000031:analogs & derivatives* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D018946:Chemokine CCL5; D053331:Ectodysplasins; C506019:Eda protein, mouse; D012333:RNA, Messenger; D009173:Mycophenolic Acid