Collagen-chitosan polymer as a scaffold for the proliferation of human adipose tissue-derived stem cells.
The architecture and biomaterial are vital for three-dimensional culture of cells in scaffolds, so collagen-chitosan scaffolds suitable for the proliferation of adipose tissue-derived stem cells (ADSCs) were fabricated in this study. Chitosan was fully mixed with collagen with different volume ratio and cross-linked. The microstructure, pore size, bibulous ability, water content, interval porosity, enzyme degradation and affinity were examined before and after cross-linking. During ADSCs cultured in scaffold, the viability and metabolic rates were measured. After 14 days, the surface markers, specific transcription factors and multi-differentiation potential were assayed to identify the stemness of expanded cells. According to the pore size, bibulous ability, interval porosity, degradation rate and affinity of the scaffold, we chose cross-linked scaffolds of 7:3 material ratio as a better scaffold for ADSCs proliferation, and ADSCs could be expanded by more than 20 times. All expanded cells still maintained stem cell characteristics and pluripotency. So our developed collagen-chitosan scaffolds can promote ADSCs adhesion, expansion, and maintain pluripotency.
Zhu
Yanxia
Y
Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Liaoning, Dalian, 116024, China.
Liu
Tianqing
T
Song
Kedong
K
Jiang
Bo
B
Ma
Xuehu
X
Cui
Zhanfeng
Z
D020341:Absorbable Implants; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents; D005947:Glucose / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*
D015703:Antigens, CD; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D019344:Lactic Acid; D003094:Collagen; D048271:Chitosan; D005947:Glucose