Apoptosis-inducing effect of akebia saponin D from the roots of Dipsacus asper Wall in U937 cells.
Methanol extracts of the root of Dipsacus asper Wall (Dipsacaceae) were found to exhibit apoptosis-inducing activities in U937 (human monocyte-like histiocytic) cells. Investigation of the active n-BuOH fraction led to the isolation of akebia saponin D (ASD). Structure was established by spectroscopic methods. Treatment of U937 cells with ASD induced apoptosis in a dose dependent manner. ASD exerted strong cytotoxicity against human and murine leukemia cells. It is significantly increased the subG1 cell population and expression of p53 and Bax gene. And also ASD enhanced NO production from RAW264.7 macrophage cells. Taken together, these results strongly indicate that ASD may exert apoptosis-inducing activity via induction of apoptosis through activation chiefly via the nitric oxide and apoptosis-related p53 and Bax gene expression. These data provide scientific evidence that Dipsacus asper Wall can be useful as a chemopreventive agent.
Jeong
Seung-Il
SI
Department of Food & Biotechnology, Woosuk University, Jeonju 565-701, Korea.
Zhou
Beng
B
Bae
Jin-Beom
JB
Kim
Nam-Seok
NS
Kim
Sun-Geun
SG
Kwon
Jin
J
Kim
Dae-Keun
DK
Shin
Tae-Yong
TY
Jeon
Hoon
H
Lim
Jong-Phil
JP
Kim
Hongjun
H
Kim
Ho Kyoung
HK
Oh
Chan-Ho
CH
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D049917:Dipsacaceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D015870:Gene Expression / Q000187:drug effects; D016158:Genes, p53 / Q000187:drug effects; D006801:Humans; D008958:Models, Molecular; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000096:biosynthesis / Q000502:physiology; D018517:Plant Roots / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D020298:U937 Cells; D051028:bcl-2-Associated X Protein / Q000235:genetics
D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; C534004:akebia saponin D; D051028:bcl-2-Associated X Protein; D009569:Nitric Oxide; D004317:Doxorubicin