Fabrication of holographic diffractive optical elements for enhancing light collection from fluorescence-based biochips.
An approach to enhancing the light collection from fluorescence emitters bound on the surface of a biochip is presented. It is based on the integration of diffractive optical elements on the underside of the chip that are essentially thick volume holograms written into a layer of photopolymer recording material. The high diffractive efficiency and angular selectivity of these types of diffractive elements make them very effective collectors of the spatially anisotropic light emitted by surface-bound fluorophores. The holographic lithography setup used to fabricate the diffractive elements is described. Results obtained using both a focused laser and light from a fluorescence spot to characterize the performance of the diffractive optical elements are presented.
Macko
Peter
P
Nanotechnology and Molecular Imaging Unit, Institute for Health and Consumer Protection, European Commission-DG Joint Research Centre, Ispra, Italy. peter.macko@jrc.it
Whelan
Maurice P
MP
D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D012680:Sensitivity and Specificity