Identification of a differentially expressed gene, ACL, between Meishan x Large White and Large White x Meishan F1 hybrids and their parents.
ATP-citrate lyase (ACL), one of the lipogenic enzymes, catalyses the formation of acetyl-coenzyme A (CoA) involved in the synthesis of fatty acid and cholesterol. In pig, very little is known about the ACL gene. In this work, the mRNA differential display technique was used to analyse the differences in gene expression between Meishan and Large White pigs and the F1 hybrids of both direct and reciprocal crosses. Our results show that among the differentially expressed genes ACL is up-regulated in the backfat of the F1 hybrids. After cloning and analysing the full-length cDNA and the 870 bp 50-flanking sequence of the porcine ACL gene, a C/T mutation at position -97 bp upstream of the transcription site was detected. Luciferase activity detection showed that this mutation changed the transcriptional activity. In F1 hybrids, the heterozygous genotype CT was more frequent than the homozygous genotypes CC and TT. Real-time PCR analysis showed that in Meishan pigs, ACL mRNA expression was more abundant in individuals with genotype CT than in those with genotype CC or TT or in Large White pigs. These results indicate that the C/T mutation affects ACL mRNA expression, probably via the activator protein 2.
Ren
Zhu-Qing
ZQ
Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China.
Wang
Yan
Y
Xu
Yong-Jie
YJ
Wang
Lin-Jie
LJ
Lei
Ming-Gang
MG
Zuo
Bo
B
Li
Feng-E
FE
Xu
De-Quan
DQ
Zheng
Rong
R
Deng
Chang-Yan
CY
Jiang
Si-Wen
SW
Xiong
Yuan-Zhua
YZ
D001275:ATP Citrate (pro-S)-Lyase / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D002678:Chimera / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D009154:Mutation; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D034421:Sus scrofa / Q000235:genetics*; D015854:Up-Regulation
D018076:DNA, Complementary; D001275:ATP Citrate (pro-S)-Lyase