[Determination of organic acids in dental plaque with high performance liquid chromatography].
OBJECTIVE
To establish determination method of formic acid, lactic acid, acetic acid and succinic acid in dental plaque with high performance liquid chromatography (HPLC).


METHODS
After the samples were centrifuged, 2 microL supernatant was transferred to a 1 mL centrifuge tube and diluted in water, then was determined with HPLC. The mixture of phosphate buffer and methanol (97:3) as mobile phase throughout the experiment. The determination of organic acid was performed on Phenomenex C18 column and at their maximum absorption wave.


RESULTS
The linear ranges of formic acid, lactic acid, acetic acid and succinic acid were 0.110-500, 0.049-500, 0.047-500, 0.084-500 microg/mL. The detection limits were 0.110, 0.049, 0.047, 0.084 microg/mL. The relative standard derivation were 9.5%, 7.9%, 4.3%, 4.2%. The average recoveries of samples were 82%-112%, 82%-102.5%, 90%-115%, 80%-110%.


CONCLUSIONS
The method was simple, quick and adapt for analysis of organic acid in dental plaque.
Zou
Xiao-li
XL
Dept. of Sanitary Technology, West China College of Public Health, Sichuan University, Chengdu 610041, China.
Zeng
Hong-yan
HY
Yan
Ci-ling
CL
Zhu
Wan-chun
WC
Li
Ji-yao
JY
D002851:Chromatography, High Pressure Liquid; D003773:Dental Plaque; D005561:Formates
D005561:Formates; C030544:formic acid