Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Streptococcus suis serotype 2.
2-Keto-3-deoxy-6-phosphogluconate (KDPG) adolase from pathogenic Streptococcus suis serotype 2 was crystallized using the hanging-drop vapour-diffusion method at 291 K. X-ray diffraction data were collected to 2.8 A resolution. The crystal belonged to space group R32, with unit-cell parameters a = b = 126.4, c = 415.9 A, alpha = beta = 90, gamma = 120 degrees . Assuming the presence of six molecules in the asymmetric unit gave a V(M) value of 2.32 A(3) Da(-1) and a solvent content of 47.12%.
Zhang
Qiangmin
Q
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Gao
Feng
F
Qi
Jianxun
J
Cheng
Hao
H
Liu
Yiwei
Y
Gao
George F
GF
D000446:Aldehyde-Lyases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D018360:Crystallography, X-Ray; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017011:Streptococcus suis / Q000201:enzymology*; D013552:Swine; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D000446:Aldehyde-Lyases; C100131:phospho-2-keto-3-deoxy-gluconate aldolase