title: Proteinase 3-processed form of the recombinant IL-32 separate domain.
abstract: Interleukin-32 (IL-32) induces a variety of proinflammatory cytokines and chemokines. The IL-32 transcript was reported originally in activated T cells; subsequently, it was demonstrated to be abundantly expressed in epithelial and endothelial cells upon stimulation with inflammatory cytokines. IL-32 is regulated robustly by other major proinflammatory cytokines, thereby suggesting that IL-32 is crucial to inflammation and immune responses. Recently, an IL-32alpha-affinity column was employed in order to isolate an IL-32 binding protein, neutrophil proteinase 3 (PR3). Proteinase 3 processes a variety of inflammatory cytokines, including TNFalpha, IL-1beta, IL-8, and IL-32, thereby enhancing their biological activities. In the current study, we designed four PR3-cleaved IL-32 separate domains, identified by potential PR3 cleavage sites in the IL-32alpha and gamma polypeptides. The separate domains of the IL-32 isoforms alpha and gamma were more active than the intrinsic alpha and gamma isoforms. Interestingly, the N-terminal IL-32 isoform gamma separate domain evidenced the highest levels of biological activity among the IL-32 separate domains.
authors.affiliation: Department of Respiratory Medicine, Konkuk University, Korea.
authors.forename: Sunjong
authors.initials: S
authors.lastname: Kim
authors.forename: Siyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Erk
authors.initials: E
authors.lastname: Her
authors.forename: Suyoung
authors.initials: S
authors.lastname: Bae
authors.forename: Jida
authors.initials: J
authors.lastname: Choi
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Hong
authors.forename: Jun
authors.initials: J
authors.lastname: Jaekal
authors.forename: Doyoung
authors.initials: D
authors.lastname: Yoon
authors.forename: Tania
authors.initials: T
authors.lastname: Azam
authors.forename: Charles A
authors.initials: CA
authors.lastname: Dinarello
authors.forename: Soohyun
authors.initials: S
authors.lastname: Kim
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D007378:Interleukins / Q000737:chemistry* / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism; D008954:Models, Biological; D053146:Myeloblastin / Q000378:metabolism*; D011014:Pneumonia / Q000097:blood / Q000378:metabolism / Q000473:pathology; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: C496971:IL32 protein, human; D007378:Interleukins; D020033:Protein Isoforms; D011994:Recombinant Proteins; D053146:Myeloblastin