title: Nanoscale imaging of molecular positions and anisotropies.
abstract: Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.
authors.affiliation: Department of Physics and Astronomy and Institute for Molecular Biophysics, 5709 Bennett Hall, University of Maine, Orono, Maine 04469, USA.
authors.forename: Travis J
authors.initials: TJ
authors.lastname: Gould
authors.forename: Mudalige S
authors.initials: MS
authors.lastname: Gunewardene
authors.forename: Manasa V
authors.initials: MV
authors.lastname: Gudheti
authors.forename: Vladislav V
authors.initials: VV
authors.lastname: Verkhusha
authors.forename: Shu-Rong
authors.initials: SR
authors.lastname: Yin
authors.forename: Julie A
authors.initials: JA
authors.lastname: Gosse
authors.forename: Samuel T
authors.initials: ST
authors.lastname: Hess
mesh_terms: D000818:Animals; D016880:Anisotropy; D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*
chemical_list: D001704:Biopolymers