Mechanical stimulation of stem cells using cyclic uniaxial strain.
The role of mechanical forces in the development and maintenance of biological tissues is well documented, including several mechanically regulated phenomena such as bone remodeling, muscular hypertrophy, and smooth muscle cell plasticity. However, the forces involved are often extremely complex and difficult to monitor and control in vivo. To better investigate the effects of mechanical forces on cells, we have developed an in vitro method for applying uniaxial cyclic tensile strain to adherent cells cultured on elastic membranes. This method utilizes a custom-designed bioreactor with a motorized cam-rotor system to apply the desired force. Here we present a step-by-step video protocol demonstrating how to assemble the various components of each "stretch chamber", including, in this case, a silicone membrane with micropatterned topography to orient the cells with the direction of the strain. We also describe procedures for sterilizing the chambers, seeding cells onto the membrane, latching the chamber into the bioreactor, and adjusting the mechanical parameters (i.e. magnitude and rate of strain). The procedures outlined in this particular protocol are specific for seeding human mesenchymal stem cells onto silicone membranes with 10 microm wide channels oriented parallel to the direction of strain. However, the methods and materials presented in this system are flexible enough to accommodate a number of variations on this theme: strain rate, magnitude, duration, cell type, membrane topography, membrane coating, etc. can all be tailored to the desired application or outcome. This is a robust method for investigating the effects of uniaxial tensile strain applied to cells in vitro.
Kurpinski
Kyle
K
Department of Bioengineering, University of California, Berkeley, USA. kkurpins@berkeley.edu
Li
Song
S
D019149:Bioreactors; D002478:Cells, Cultured; D003584:Cytological Techniques; D006801:Humans; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D012828:Silicones; D013314:Stress, Mechanical
D008567:Membranes, Artificial; D012828:Silicones