Label-free in vivo optical imaging of microvasculature and oxygenation level.
The ability to perform high-resolution imaging of microvasculature and its oxygenation is very important in studying early tumor development. Toward this goal, we improved upon our excited state absorption (ESA)-based imaging technique to allow us to not only image hemoglobin directly but also differentiate between oxy- and deoxyhemoglobin in tissue. We demonstrate the separation of arterioles from venules in a live nude mouse ear using our imaging technique.
Fu
Dan
D
Matthews
Thomas E
TE
Ye
Tong
T
Piletic
Ivan R
IR
Warren
Warren S
WS
D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D004423:Ear / Q000098:blood supply / Q000502:physiology; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D055806:Microvessels / Q000166:cytology* / Q000502:physiology*; D010092:Oximetry / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology
D010100:Oxygen