title: Lipoarabinomannan of Mycobacterium: mannose capping by a multifunctional terminal mannosyltransferase.
abstract: Biosynthesis of phosphatidylinositol (PI)-containing lipoarabinomannan (LAM) and lipomannan (LM) of Mycobacterium spp. follows a conserved pathway involving multiple membrane-associated, substrate-specific mannosyltransferases (ManTs) responsible for the sequential addition of alpha-mannopyranosyl (Manp) units donated by decaprenyl-P-Manp on the periplasmic side of the plasma membrane. Because of their receptor-binding and immunomodulatory properties, the alpha(1-->2)-linked di- and tri-Manp motifs that functionalize the nonreducing arabinan termini of LAM (ManLAM) in Mycobacterium tuberculosis are of crucial importance. We now show that the M. tuberculosis ManT, Rv2181, is required for the addition of these alpha(1-->2)-linked Manp residues but also at other locations of the LAM molecule. Structural analyses of the LM and LAM variants produced by a M. tuberculosis Rv2181 knockout mutant revealed the presence of but a single Manp residue on the nonreducing arabinan termini of LAM and also a complete absence of alpha(1-->2)-linked Man branching on the mannan backbones of LM and LAM. A recombinant strain was constructed in ManLAM-deficient Mycobacterium smegmatis that coexpressed Rv2181 and Rv1635c-the ManT responsible for the addition of the first Manp capping residue of ManLAM. Analysis revealed LAM termini fully capped with di- and tri-Manp motifs in addition to alpha(1-->2)Man branching on the mannan backbones of LM and LAM, confirming the involvement of the alpha(1-->2)ManT Rv2181 in the dual role of Man capping and mannan-core branching, and in the process generated a rapidly growing, ManLAM-containing strain, a tool for the study of the role of ManLAM in the pathogenesis of tuberculosis.
authors.affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Devinder
authors.initials: D
authors.lastname: Kaur
authors.forename: Andrés
authors.initials: A
authors.lastname: Obregón-Henao
authors.forename: Ha
authors.initials: H
authors.lastname: Pham
authors.forename: Delphi
authors.initials: D
authors.lastname: Chatterjee
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Brennan
authors.forename: Mary
authors.initials: M
authors.lastname: Jackson
mesh_terms: D000483:Alleles; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D008364:Mannosyltransferases / Q000378:metabolism*; D008745:Methylation; D009154:Mutation / Q000235:genetics; D020102:Mycobacterium smegmatis / Q000201:enzymology; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000254:growth & development; D010641:Phenotype; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008070:Lipopolysaccharides; C050016:lipoarabinomannan; C064351:lipomannan; D008364:Mannosyltransferases; D008358:Mannose