title: Inactivation of Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes on stored iceberg lettuce by aqueous chlorine dioxide treatment.
abstract: Inactivation of Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes in iceberg lettuce by aqueous chlorine dioxide (ClO(2)) treatment was evaluated. Iceberg lettuce samples were inoculated with approximately 7 log CFU/g of E. coli O157:H7, S. typhimurium, and L. monocytogenes. Iceberg lettuce samples were then treated with 0, 5, 10, or 50 ppm ClO(2) solution and stored at 4 degrees C. Aqueous ClO(2) treatment significantly decreased the populations of pathogenic bacteria on shredded lettuce (P < 0.05). In particular, 50 ppm ClO(2) treatment reduced E. coli O157:H7, S. typhimurium, and L. monocytogenes by 1.44, 1.95, and 1.20 log CFU/g, respectively. The D(10)-values of E. coli O157:H7, S. typhimurium, and L. monocytogenes in shredded lettuce were 11, 26, and 42 ppm, respectively. The effect of aqueous ClO(2) treatment on the growth of pathogenic bacteria during storage was evaluated, and a decrease in the population size of these pathogenic bacteria was observed. Additionally, aqueous ClO(2) treatment did not affect the color of lettuce during storage. These results suggest that aqueous ClO(2) treatment can be used to improve the microbial safety of shredded lettuce during storage.
authors.affiliation: Department of Food Science and Technology, Chungnam National University, Daejeon, 305-764, Korea.
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Jiyong
authors.initials: J
authors.lastname: Park
authors.forename: Jonghyun
authors.initials: J
authors.lastname: Park
authors.forename: Myongsoo
authors.initials: M
authors.lastname: Chung
authors.forename: Kisung
authors.initials: K
authors.lastname: Kwon
authors.forename: Kyungsook
authors.initials: K
authors.lastname: Chung
authors.forename: Misun
authors.initials: M
authors.lastname: Won
authors.forename: Kyung Bin
authors.initials: KB
authors.lastname: Song
mesh_terms: D017606:Chlorine Compounds / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000528:radiation effects; D005511:Food Handling; D005638:Fruit / Q000382:microbiology / Q000592:standards; D018545:Lactuca / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000528:radiation effects; D010087:Oxides / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014675:Vegetables / Q000382:microbiology / Q000592:standards
chemical_list: D017606:Chlorine Compounds; D004202:Disinfectants; D010087:Oxides; C025109:chlorine dioxide