Bioluminescent indicators for Ca2+ based on split Renilla luciferase complementation in living cells.
Genetically encoded bioluminescent indicators for intracellular Ca2+ are described here with CaM-M13 interaction-induced complementation of split Renilla luciferase. The Ca2+-induced interaction between CaM and M13 leads to complementation of the N- and C-terminal halves of split Renilla luciferase in living cells. This intramolecular interaction results in the spontaneous and simultaneous emission of bioluminescence split Renilla luciferase. This is how intracellular Ca2+ is illuminated with the intramolecular complementation of split Renilla luciferase. The Ca2+-dependent spontaneous and simultaneous emission of bioluminescence promises to reveal Ca2+ dynamics in living cells, and also in vivo using the present indicators.
Kaihara
Asami
A
Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda, Tokyo 101-0062, Japan. kaihara.bip@mri.tmd.ac.jp
Umezawa
Yoshio
Y
Furukawa
Tetsushi
T
D002118:Calcium / Q000032:analysis*; D002147:Calmodulin; D045744:Cell Line, Tumor; D006801:Humans; D049410:Luciferases, Renilla; D008164:Luminescent Proteins; D015336:Molecular Probe Techniques; D011993:Recombinant Fusion Proteins
D002147:Calmodulin; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049410:Luciferases, Renilla; D002118:Calcium