Detection of herpesvirus and adenovirus co-infections with diagnostic DNA-microarrays.
In immunocompromised patients, the diagnosis of infections with herpesviruses and adenoviruses relies mainly on PCR amplification of viral genomic DNA from clinical samples. In the case of co-infections with two or more viruses, single amplification of viral DNA from clinical samples has proven to be time-consuming and expensive, hampering the efficient diagnosis and therapy of viral co-infections. In this study, a diagnostic DNA-microarray allowing simultaneous detection of herpes simplex virus types 1 and 2 (HSV 1/2), varicella zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus-6 types A and B (HHV-6 A/B), and adenovirus in clinical samples was developed and validated. The assay displays a high analytical sensitivity (10genomeequivalents(GE)/reaction) and specificity, being cost-effective and time-saving. Because the DNA-microarray uses the same analytical conditions as real-time quantitative PCR, it can be used as a screening device for multiple viral infections, followed by selective viral load measurement depending on the clinical context. Those features make the DNA-microarray an attractive device for the management of viral infections in immunocompromised patients.
Müller
René
R
The Calicilab, Institut für Virologie, Medizinische Fakultät Carl Gustav Carus, Dresden, Germany.
Ditzen
Anette
A
Hille
Kristin
K
Stichling
Markus
M
Ehricht
Ralf
R
Illmer
Thomas
T
Ehninger
Gerhard
G
Rohayem
Jacques
J
D000258:Adenovirus Infections, Human / Q000150:complications / Q000175:diagnosis / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification / Q000502:physiology; D006564:Herpesviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000150:complications / Q000175:diagnosis / Q000821:virology; D006801:Humans; D016867:Immunocompromised Host; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D033581:Stem Cell Transplantation / Q000009:adverse effects; D019562:Viral Load
D004279:DNA, Viral