title: Investigation of solute concentrations in a 3D model of intervertebral disc.
abstract: As the disc is the largest avascular structure in the body, disc cells depend for their normal function on an adequate supply of nutrients (oxygen and glucose) and the removal of metabolic by-products (lactic acid) via blood vessels at the cartilaginous endplates and annulus periphery. Concentration gradients develop depending on the balance between the rates of transport and rates of cellular activity. Since consumption and production rates are coupled via extracellular pH, the gradients are interdependent. This is a novel model study which takes into account the realistic 3D geometry of a L5-S1 lumbar disc in solving the nonlinear coupled diffusion equations. Effects of perturbations (calcification, sclerosis) in endplates, increases in cell metabolic rates following growth factor injection and changes in lumbar posture (kyphotic or lordotic) on extreme values of nutrient and metabolite concentrations and their spatial locations are investigated. Solute concentrations, particularly those of glucose, substantially diminish as a consequence of disturbances in supply at the endplates, increases in cell metabolic rate and more lordotic postures. Results, when compared to those from simplified axisymmetric models, demonstrate the importance of consideration of realistic 3D disc geometry.
authors.affiliation: Department of Mechanical Engineering, Ecole Polytechnique, P.O. Box 6079, Station 'centre-ville', Montreal, QC H3C 3A7, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Mokhbi Soukane
authors.forename: A
authors.initials: A
authors.lastname: Shirazi-Adl
authors.forename: J P G
authors.initials: JP
authors.lastname: Urban
mesh_terms: D019902:Chondrocytes / Q000378:metabolism; D004058:Diffusion; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D020342:Finite Element Analysis; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007403:Intervertebral Disc / Q000166:cytology* / Q000378:metabolism*; D019344:Lactic Acid / Q000032:analysis / Q000378:metabolism; D008161:Lumbosacral Region; D008954:Models, Biological; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D011187:Posture; D012996:Solutions / Q000378:metabolism
chemical_list: D012996:Solutions; D019344:Lactic Acid; D005947:Glucose; D010100:Oxygen