Proteomic analysis of protein deposits on worn daily wear silicone hydrogel contact lenses.
OBJECTIVE
Previous studies have demonstrated deposition of tear proteins onto worn contact lenses. In this study, we used proteomic techniques to analyze the protein deposits extracted from worn daily wear silicone hydrogel contact lenses in combination with different lens care solutions.


METHODS
Worn lenses were collected and protein deposits extracted using urea and surfactant. Protein extracts were desalted, concentrated, and then separated using one-dimensional gel electrophoresis. Individual protein components in extracts were identified using liquid chromatography combined with tandem mass spectrometry (LC-MS-MS) after trypsin digestion.


RESULTS
One-dimensional gel electrophoresis revealed that lysozyme and other small proteins (around 20 kDa) were the most abundant proteins in the extracts. LC-MS-MS revealed a wide array of proteins in lens extracts with lysozyme and lipocalin 1 being the most commonly identified in deposit extracts.


CONCLUSIONS
Worn contact lenses deposit a wide array of proteins from tear film and other sources. Protein deposit profiles varied and were specific for each contact lens material.
Zhao
Zhenjun
Z
Institute for Eye Research, Sydney, NSW, Australia. z.zhao@ier.org.au
Wei
Xiaojia
X
Aliwarga
Yulina
Y
Carnt
Nicole A
NA
Garrett
Qian
Q
Willcox
Mark D P
MD
D002853:Chromatography, Liquid; D018639:Contact Lens Solutions / Q000737:chemistry; D003262:Contact Lenses, Extended-Wear; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D013058:Mass Spectrometry; D040901:Proteomics; D012828:Silicones / Q000737:chemistry*
D018639:Contact Lens Solutions; D005136:Eye Proteins; D012828:Silicones; C042434:tear proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate