title: Application of a translational profiling approach for the comparative analysis of CNS cell types.
abstract: Comparative analysis can provide important insights into complex biological systems. As demonstrated in the accompanying paper, translating ribosome affinity purification (TRAP) permits comprehensive studies of translated mRNAs in genetically defined cell populations after physiological perturbations. To establish the generality of this approach, we present translational profiles for 24 CNS cell populations and identify known cell-specific and enriched transcripts for each population. We report thousands of cell-specific mRNAs that were not detected in whole-tissue microarray studies and provide examples that demonstrate the benefits deriving from comparative analysis. To provide a foundation for further biological and in silico studies, we provide a resource of 16 transgenic mouse lines, their corresponding anatomic characterization, and translational profiles for cell types from a variety of central nervous system structures. This resource will enable a wide spectrum of molecular and mechanistic studies of both well-known and previously uncharacterized neural cell populations.
authors.affiliation: Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA.
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Doyle
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Dougherty
authors.forename: Myriam
authors.initials: M
authors.lastname: Heiman
authors.forename: Eric F
authors.initials: EF
authors.lastname: Schmidt
authors.forename: Tanya R
authors.initials: TR
authors.lastname: Stevens
authors.forename: Guojun
authors.initials: G
authors.lastname: Ma
authors.forename: Sujata
authors.initials: S
authors.lastname: Bupp
authors.forename: Prerana
authors.initials: P
authors.lastname: Shrestha
authors.forename: Rajiv D
authors.initials: RD
authors.lastname: Shah
authors.forename: Martin L
authors.initials: ML
authors.lastname: Doughty
authors.forename: Shiaoching
authors.initials: S
authors.lastname: Gong
authors.forename: Paul
authors.initials: P
authors.lastname: Greengard
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Heintz
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002490:Central Nervous System / Q000378:metabolism; D022202:Chromosomes, Artificial, Bacterial / Q000378:metabolism; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D009474:Neurons / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D014176:Protein Biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012270:Ribosomes / Q000378:metabolism
chemical_list: D049452:Green Fluorescent Proteins