Phenotypic and genotypic characterization of Arcanobacterium haemolyticum isolates from infections of horses.
The present study was designed to characterize phenotypically and genotypically seven Arcanobacterium haemolyticum strains obtained from infections of six horses. All seven strains showed the cultural and biochemical properties typical of A. haemolyticum and were susceptible to most of the antibiotics tested. The species identification could be confirmed by amplification and sequencing of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region and by PCR amplification of species-specific parts of the gene encoding phospholipase D in A. haemolyticum. Use of the latter could possibly improve future identification of this generally human pathogenic bacterial species which, according to the present results, seems to occur also in infections of horses.
Hassan
Abdulwahed Ahmed
AA
De Gezondheidsdienst voor Dieren, Postbus 9, 7400 AA Deventer, The Netherlands.
Ulbegi-Mohyla
Hivda
H
Kanbar
Talah
T
Alber
Jörg
J
Lämmler
Christoph
C
Abdulmawjood
Amir
A
Zschöck
Michael
M
Weiss
Reinhard
R
D000193:Actinomycetales Infections / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D055667:Arcanobacterium / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000235:genetics; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011817:Rabbits; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010739:Phospholipase D