MAPPIT: a versatile tool to study cytokine receptor signalling.
MAPPIT (mammalian protein-protein interaction trap) is a cytokine receptor-based two-hybrid method that operates in intact mammalian cells. A bait is fused C-terminally to a STAT (signal transducer and activator of transcription) recruitment-deficient receptor, whereas the prey is linked to functional STAT-binding sites. When bait and prey interact a ligand-dependent complementation of the STAT recruitment deficiency occurs, leading to activation of a STAT-responsive reporter. MAPPIT is very well suited to study protein interactions involving activated cytokine receptors as the technique allows modification of the bait protein in a physiologically optimal environment.
Lemmens
Irma
I
Department of Medical Protein Research, VIB, Ghent University, Ghent, Belgium.
Lievens
Sam
S
Tavernier
Jan
J
D000818:Animals; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D018121:Receptors, Cytokine / Q000378:metabolism*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
D016207:Cytokines; D018121:Receptors, Cytokine