title: Na+, K+-ATPase activity and subunit isoform protein abundance: effects of antenatal glucocorticoids in the frontal cerebral cortex and renal cortex of ovine fetuses.
abstract: We examined the effects of single and multiple maternal glucocorticoid courses on cerebral cortical (CC) and renal cortical (RC) Na(+),K(+)-ATPase activity and protein isoform abundance in fetal sheep. Ewes received four dexamethasone or placebo injections in the single course (SC) groups, and the same treatment once a week for five-weeks in the multiple course (MC) groups. CC Na(+),K(+)-ATPase a(2)-abundance was higher (P<0.05) and beta(2)-abundance lower in the SC dexamethasone than placebo group, but Na(+),K(+)-ATPase activity did not change. CC Na(+),K(+)-ATPase activity, a(1)-, beta(1) -, and beta(2)-abundance were lower in the MC dexamethasone than placebo group, but a(2)- and a(3)-abundance did not change. Both dexamethasone courses did not affect CC cell number. RC Na(+),K(+)-ATPase activity, a(1)- and beta(1) -abundance were higher in the MC dexamethasone than placebo group, but did not change in the SC dexamethasone group. We conclude MC, but not a SC of dexamethasone, affect fetal cerebral and renal Na(+),K(+)-ATPase, and MC result in differential effects on Na(+),K(+)-ATPase in these organs.
authors.affiliation: Warren Alpert Medical School of Brown University, Department of Pediatrics, Women & Infants' Hospital of Rhode Island, Providence, Rhode Island 02905, USA.
authors.forename: Najma S
authors.initials: NS
authors.lastname: Mehter
authors.forename: Grazyna B
authors.initials: GB
authors.lastname: Sadowska
authors.forename: Shadi N
authors.initials: SN
authors.lastname: Malaeb
authors.forename: Barbara S
authors.initials: BS
authors.lastname: Stonestreet
mesh_terms: D000818:Animals; D002452:Cell Count; D002540:Cerebral Cortex / Q000187:drug effects* / Q000196:embryology / Q000201:enzymology; D003907:Dexamethasone / Q000008:administration & dosage* / Q000378:metabolism; D004334:Drug Administration Schedule; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage* / Q000378:metabolism; D007273:Injections, Intramuscular; D007672:Kidney Cortex / Q000187:drug effects* / Q000196:embryology / Q000201:enzymology; D008431:Maternal-Fetal Exchange; D009474:Neurons / Q000187:drug effects / Q000201:enzymology; D011247:Pregnancy; D021122:Protein Subunits; D012756:Sheep; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D005938:Glucocorticoids; D021122:Protein Subunits; D003907:Dexamethasone; D000254:Sodium-Potassium-Exchanging ATPase