title: Identification of the HERV-K gag antigen in prostate cancer by SEREX using autologous patient serum and its immunogenicity.
abstract: The prostate cancer HERV-K gag-related NGO-Pr-54 antigen was identified by SEREX analysis using autologous patient serum. NGO-Pr-54 mRNA was observed to be faintly expressed in normal prostate and strongly expressed in a variety of cancers, including ovarian cancer (5/8), prostate cancer (6/9), and leukemia (5/14). A phage plaque assay showed that a strong reaction was constantly observed with clone ZH042 in which the 5' end of NGO-Pr-54 is deleted, suggesting that it contained the sequence coding for the protein product. A TI-35 mAb was produced using a recombinant protein (438 aa) deduced from the sequence of ZH042. Transfection of clone ZH042 into 293T cells resulted in the production of an approximately 50-kDa molecule visualized by Western blotting. Natural production of the molecule was confirmed in a SK-MEL-23 melanoma cell line. An indirect immunofluorescence assay showed that NGO-Pr-54 protein was expressed on the cell surface as well as in the cytoplasm. Cell surface expression was confirmed by flow cytometry using the TI-35 mAb. The antibody response against NGO-Pr-54 was observed in patients with bladder (5.1%), liver (4.1%), lung (3.4%), ovarian (5.6%), and prostate (4.2%) cancer, as well as with malignant melanoma (13.2%).
authors.affiliation: Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Ishida
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Obata
authors.forename: Nobuya
authors.initials: N
authors.lastname: Ohara
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsushita
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Sato
authors.forename: Akiko
authors.initials: A
authors.lastname: Uenaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Saika
authors.forename: Takako
authors.initials: T
authors.lastname: Miyamura
authors.forename: Kosuke
authors.initials: K
authors.lastname: Chayama
authors.forename: Yurika
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Hisashi
authors.initials: H
authors.lastname: Wada
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yamashita
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Morishima
authors.forename: Lloyd J
authors.initials: LJ
authors.lastname: Old
authors.forename: Eiichi
authors.initials: E
authors.lastname: Nakayama
mesh_terms: D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D020077:Endogenous Retroviruses; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D015972:Gene Expression Regulation, Neoplastic; D015683:Gene Products, gag; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007938:Leukemia / Q000235:genetics / Q000276:immunology; D008297:Male; D008545:Melanoma / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000276:immunology; D011467:Prostate / Q000276:immunology / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015683:Gene Products, gag; D007106:Immune Sera; C534523:NGO-Pr-54 protein, human; D009363:Neoplasm Proteins; D011994:Recombinant Proteins