Encystment of Azotobacter nigricans grown diazotrophically on kerosene as sole carbon source.
Encystment of Azotobacter nigricans was induced by its diazotrophic cultivation on kerosene. Its growth and nitrogenase activity were affected by kerosene in comparison to cultures grown on sucrose. Electron microscopy of vegetative cells showed that when nitrogenase activity was higher and the poly-beta-hydroxybutyrate granules were not present to a significant extent, peripheral bodies were abundant. After 8 days of culture on kerosene, the presence of cysts with intracellular bunches of poly-beta-hydroxybutyrate granules was observed. Germination of cysts bears germinating multicelled yet unbroken capsule cysts with up to three cells inside. This is the first report of encystment induction of Azotobacter species grown on kerosene.
García-Esquivel
Gabriela
G
Biotechnology and Bioengineering Department, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, C. P. 07360 Mexico D. F., Mexico.
Calva-Calva
Graciano
G
Ferrera-Cerrato
Ronald
R
Fernández-Linares
Luis Carlos
LC
Vázquez
Refugio Rodríguez
RR
Esparza-García
Fernando José
FJ
D001395:Azotobacter / Q000166:cytology* / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000032:analysis; D007648:Kerosene; D009591:Nitrogenase / Q000378:metabolism; D011091:Polyesters / Q000032:analysis; D013171:Spores, Bacterial / Q000378:metabolism / Q000648:ultrastructure; D000094122:Polyhydroxybutyrates
D002244:Carbon; D006885:Hydroxybutyrates; D007648:Kerosene; D009591:Nitrogenase; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates