title: Promiscuous interaction between gold-specific T cells and APCs in gold allergy.
abstract: Gold compounds clinically used as immunomodulators have high potential to evoke hypersensitivity reactions as an adverse effect. To explore the mechanism of gold allergy, we immunologically characterized T cells infiltrating skin rashes and generated 44 gold-specific T cell clones and lines from a rheumatoid arthritis patient who developed skin rashes and systemic symptoms after gold treatment. CD4(+) and CD8(+) cells predominantly infiltrating the skin rashes and some of the T cell clones and lines shared common Vbetas. These cells exhibited Th0-like, Th2-like, and Tc1-like cytokine profiles, and showed chemotactic activities for thymus and activation-regulated chemokine and IFN-gamma-inducible protein-10 corresponding to the cytokine profiles. T cell recognition of gold consisted of MHC-restricted and MHC-independent pathways. Blocking studies with anti-MHC Abs indicated that the groove of MHC in APCs, where Ags should ordinarily be settled, did not serve as a conjugating site of gold for these T cells in certain cases. These observations raise the possibility that gold-specific CD4(+) and CD8(+) T cells and APCs promiscuously interact under stimulation with gold, resulting in various clinical manifestations in gold allergy.
authors.affiliation: Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan. hihashiz@hama-med.ac.jp
authors.forename: Hideo
authors.initials: H
authors.lastname: Hashizume
authors.forename: Naohiro
authors.initials: N
authors.lastname: Seo
authors.forename: Taisuke
authors.initials: T
authors.lastname: Ito
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takigawa
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yagi
mesh_terms: D000368:Aged; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000473:pathology; D018501:Antirheumatic Agents / Q000008:administration & dosage / Q000009:adverse effects*; D002450:Cell Communication / Q000187:drug effects / Q000276:immunology*; D054357:Chemokine CXCL10 / Q000276:immunology; D002633:Chemotaxis / Q000187:drug effects / Q000276:immunology; D004342:Drug Hypersensitivity / Q000276:immunology* / Q000473:pathology; D005260:Female; D006046:Gold; D006052:Gold Sodium Thiomalate / Q000008:administration & dosage / Q000009:adverse effects*; D006649:Histocompatibility Antigens / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000276:immunology* / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000473:pathology; D018418:Th2 Cells / Q000276:immunology* / Q000473:pathology; D013950:Thymus Gland / Q000276:immunology / Q000473:pathology
chemical_list: D018501:Antirheumatic Agents; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D006649:Histocompatibility Antigens; D006052:Gold Sodium Thiomalate; D006046:Gold