A new abietane diterpenoid from Isodon inflexus.
A new ent-abietane diterpenoid, 3alpha,6beta-dihydroxy-7,17-dioxo-ent-abieta-15(16)-ene (1), and three known ent-kaurane diterpenids, kamebacetal A (2), kamebakaurin (3), and excisanin A (4), and a known triterpenoid, ursolic acid (5), were isolated from the aerial parts of Isodon inflexus. Their chemical structures were determined by extensive analysis of spectroscopic data including 1D-and 2D-NMR experiments. All isolates (1-5) were evaluated for their potential to inhibit LPS-induced nitric oxide production in RAW264.7 cells. Of these, compounds 1-4 inhibited the production of NO with IC(50) values ranging from 1.0 to 26.5 microM.
Lee
Chul
C
College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea.
Hong
Seong Su
SS
Han
Xiang Hua
XH
Jin
Qinghao
Q
Li
Dayu
D
Kim
Tae Ook
TO
Kim
Hyun-Kyu
HK
Lee
Jeongrai
J
Kwon
Suk Hyung
SH
Kim
Yun-Bae
YB
Lee
Mi Kyeong
MK
Hwang
Bang Yeon
BY
D045784:Abietanes / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D037602:Isodon / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013778:Tetrazolium Salts; D013844:Thiazoles
C534002:3,6-dihydroxy-7,17-dioxo-ent-abieta-15(16)-ene; D045784:Abietanes; D004224:Diterpenes; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; D009569:Nitric Oxide; C022616:thiazolyl blue