Normal and osteoporotic human osteoblast behaviour after 1,25-dihydroxy-vitamin D(3) stimulation.
In order to examine the effects of vitamin D on osteoblast function and to evaluate if osteoporotic and normal osteoblasts show a different behaviour in response to vitamin D, this report investigates the changes in osteocalcin production, after 1,25-dihydroxy-vitamin D(3) stimulation of cultured osteoblasts derived from osteoporotic patients. Our results indicate an inadequate osteoblastic function in osteoporosis and demostrate that 1,25-dihydroxy-vitamin D(3) can stimulate the metabolic activity of human osteoblasts in vitro. Considering that osteoporotic bone samples were representative of senile osteoporosis, our results may indicate a different metabolic phenotype in osteoporotic osteoblasts compared with normal osteoblasts. The increased osteocalcin production after 1,25-dihydroxy-vitamin D(3) stimulation of osteoporotic osteoblasts suggests a reduced, but not absent, anabolic function in senile osteoporotic osteoblasts. The results of this study confirm the validity of vitamin D(3) to treat senile osteoporosis and suggest the need of higher vitamin D(3) intake in senile osteoporotic patients than in younger subjects.
Maruotti
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N
Departement of Rheumatology, University of Foggia Medical School, D'Avanzo Hospital, Viale degli Aviatori, 1, 71100, Foggia, Italy.
Corrado
A
A
Grano
M
M
Colucci
S
S
Cantatore
F P
FP
D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D016022:Case-Control Studies; D019070:Cell Lineage; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D015675:Osteocalcin / Q000096:biosynthesis / Q000378:metabolism*; D010024:Osteoporosis / Q000378:metabolism*; D013997:Time Factors; D014343:Trypan Blue / Q000378:metabolism; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology
D004396:Coloring Agents; C118756:dihydroxy-vitamin D3; D015675:Osteocalcin; D014807:Vitamin D; D000469:Alkaline Phosphatase; D014343:Trypan Blue