title: Structure of the capsid amino-terminal domain from the betaretrovirus, Jaagsiekte sheep retrovirus.
abstract: Jaagsiekte sheep retrovirus is a betaretrovirus and the causative agent of pulmonary adenocarcinoma, a transmissible lung tumour of sheep. Here we report the crystal structure of the capsid amino-terminal domain and examine the self-association properties of Jaagsiekte sheep retrovirus capsid. We find that the structure is remarkably similar to the amino-terminal domain of the alpharetrovirus, avian leukosis virus, revealing a previously undetected evolutionary similarity. Examination of capsid self-association suggests a mode of assembly not driven by the strong capsid carboxy-terminal domain interactions that characterise capsid assembly in the lentiviruses. Based on these data, we propose this structure provides a model for the capsid of betaretroviruses including the HML-2 family of endogenous human betaretroviruses.
authors.affiliation: Division of Molecular Structure, National Institute for Medical Research, the Ridgeway, Mill Hill, London NW7 1AA, UK.
authors.forename: Gulnahar B
authors.initials: GB
authors.lastname: Mortuza
authors.forename: David C
authors.initials: DC
authors.lastname: Goldstone
authors.forename: Clare
authors.initials: C
authors.lastname: Pashley
authors.forename: Lesley F
authors.initials: LF
authors.lastname: Haire
authors.forename: Massimo
authors.initials: M
authors.lastname: Palmarini
authors.forename: William R
authors.initials: WR
authors.lastname: Taylor
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Stoye
authors.forename: Ian A
authors.initials: IA
authors.lastname: Taylor
mesh_terms: D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D022141:Jaagsiekte sheep retrovirus / Q000737:chemistry*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012996:Solutions
chemical_list: D036022:Capsid Proteins; D012996:Solutions