title: Prokineticin receptor 2 expression identifies migrating neuroblasts and their subventricular zone transient-amplifying progenitors in adult mice.
abstract: The adult subventricular zone (SVZ) contains progenitors cells, which continually give rise to new neurons that migrate along the rostral migratory stream (RMS) to the olfactory bulbs (OB). Prokineticin receptor 2 (ProKR2) is a G-protein-coupled receptor that plays an essential role in this migration process. However, the identity of the prokr2-expressing cells has not yet been clearly established. Here, we have characterized in detail the identity of the prokr2-expressing cells in the SVZ/RMS/OB pathway in adult mice. In the SVZ, accumulation of prokr2 transcripts was detected in almost all migrating neuroblasts or type A cells as well as in a large population of their precursors, the rapidly dividing type C cells. Moreover, we observed that, in dissociated SVZ cells from Mash1::GFP postnatal mice, ProKR2 protein is also present in type C and type A cells. We found that, along the RMS and in the OB, prokr2 expression was restricted to migrating type A cells and was absent in astrocytes. Finally, we observed a highly marked decrease of prokr2 expression in Mash1-/- mutant mice, suggesting that this transcription factor directly or indirectly regulates prokr2 expression. Although the expression of ProKR2 in migrating type A cells is in good agreement with the essential role played by this receptor during this migration process, its expression in a large population of their progenitors suggests an additional function for ProKR2, providing novel insights into the role of ProKR2/ProK2 signalling in adult neurogenesis.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale Equipe Avenir, Zebrafish Neurogenesis, IFR70, Hôpital de la Pitié-Salpêtrière, 75651 Paris, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Puverel
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nakatani
authors.forename: Carlos
authors.initials: C
authors.lastname: Parras
authors.forename: Nadia
authors.initials: N
authors.lastname: Soussi-Yanicostas
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000166:cytology* / Q000502:physiology; D002465:Cell Movement; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009434:Neural Pathways; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; C499317:Ascl1 protein, mouse; C525016:PKR2 protein, mouse