title: Reduction of IgE binding and nonpromotion of Aspergillus flavus fungal growth by simultaneously silencing Ara h 2 and Ara h 6 in peanut.
abstract: The most potent peanut allergens, Ara h 2 and Ara h 6, were silenced in transgenic plants by RNA interference. Three independent transgenic lines were recovered after microprojectile bombardment, of which two contained single, integrated copies of the transgene. The third line contained multiple copies of the transgene. Ara h 2 expression was significantly suppressed in all three lines, whereas Ara h 6 was reduced in two lines. Expression of peanut allergens Ara h 1 and Ara h 3 was not noticeably affected. Significant reduction of human IgE binding to Ara h 2 and Ara h 6 also was observed. Seed weight and germination data from transgenic and nontransgenic segregants showed no significant differences. Data collected from in vitro Aspergillus flavus infection indicate no significant difference in fungal growth between the transgenic lines and the nontransgenic controls. These data suggest that silencing Ara h 2 and Ara h 6 is a feasible approach to produce hypoallergenic peanut.
authors.affiliation: Department of Horticulture, University of Georgia, 31793, USA.
authors.forename: Ye
authors.initials: Y
authors.lastname: Chu
authors.forename: Paola
authors.initials: P
authors.lastname: Faustinelli
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Ramos
authors.forename: Martin
authors.initials: M
authors.lastname: Hajduch
authors.forename: Severin
authors.initials: S
authors.lastname: Stevenson
authors.forename: Jay J
authors.initials: JJ
authors.lastname: Thelen
authors.forename: Soheila J
authors.initials: SJ
authors.lastname: Maleki
authors.forename: Hsiaopo
authors.initials: H
authors.lastname: Cheng
authors.forename: Peggy
authors.initials: P
authors.lastname: Ozias-Akins
mesh_terms: D055516:2S Albumins, Plant / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000485:Allergens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D010367:Arachis / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000382:microbiology*; D001231:Aspergillus flavus / Q000254:growth & development*; D005512:Food Hypersensitivity / Q000276:immunology*; D020868:Gene Silencing; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment
chemical_list: D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant; C500890:Ara h 2 allergen, Arachis hypogaea; C492737:Ara h 6 allergen, Arachis hypogaea; D006023:Glycoproteins; D010940:Plant Proteins; D007073:Immunoglobulin E