Real-time PCR analysis of HIV-1 replication post-entry events.
The reverse transcriptase enzyme plays an essential role in the HIV-1 life cycle by converting a single-stranded viral RNA genome into a double-stranded viral DNA through a complex process known as reverse transcription. The resulting double-stranded DNA is integrated into the host chromosome to form a provirus. A small proportion of the viral DNAs form dead-end circular products, which nevertheless can serve as useful surrogate markers for monitoring viral replication. Utilizing real-time PCR technology, it is possible to track and quantify different stages of the reverse transcription process, the proviruses, and the nonintegrated dead-end reverse transcription products.
Mbisa
Jean L
JL
HIV-Drug Resistance Program, NCI-Frederick, Frederick, MD, USA.
Delviks-Frankenberry
Krista A
KA
Thomas
James A
JA
Gorelick
Robert J
RJ
Pathak
Vinay K
VK
D002460:Cell Line; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D014779:Virus Replication
D012367:RNA, Viral