title: Concentrating rotaviruses from water samples using monolithic chromatographic supports.
abstract: Rotaviruses are the leading cause of diarrhoea in infants around the globe and, under certain conditions they can be present in drinking water sources and systems. Ingestion of 10-100 viral particles is enough to cause disease, emphasizing the need for sensitive diagnostic methods. In this study we have optimized the concentration of rotavirus particles using methacrylate monolithic chromatographic supports. Different surface chemistries and mobile phases were tested. A strong anion exchanger and phosphate buffer (pH 7) resulted in the highest recoveries after elution of the bound virus with 1M NaCl. Using this approach, rotavirus particles spiked in 1l volumes of tap or river water were efficiently concentrated. The developed concentration method in combination with a real time quantitative polymerase chain reaction assay detected rotavirus concentrations as low as 100 rotavirus particles/ml.
authors.affiliation: Department of Biotechnology and Systems Biology, National Institute of Biology, Vecna pot 111, SI-1000 Ljubljana, Slovenia. ion.gutierrez@nib.si
authors.forename: Ion
authors.initials: I
authors.lastname: Gutiérrez-Aguirre
authors.forename: Marko
authors.initials: M
authors.lastname: Banjac
authors.forename: Andrej
authors.initials: A
authors.lastname: Steyer
authors.forename: Mateja
authors.initials: M
authors.lastname: Poljsak-Prijatelj
authors.forename: Matjaz
authors.initials: M
authors.lastname: Peterka
authors.forename: Ales
authors.initials: A
authors.lastname: Strancar
authors.forename: Maja
authors.initials: M
authors.lastname: Ravnikar
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D005243:Feces / Q000821:virology; D005618:Fresh Water / Q000032:analysis / Q000821:virology*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D008689:Methacrylates