Quantitative determination of atorvastatin and para-hydroxy atorvastatin in human plasma by LC-MS-MS.
A specific, sensitive, and fast method based on high-performance liquid chromatography coupled to tandem mass spectrometry was developed for the determination of atorvastatin and para-hydroxy atorvastatin in human plasma. Solid-phase extraction was used to isolate the compounds from human plasma followed by injection of the extracts onto a C18 column with isocratic elution. The lower limits of quantitation was 0.229 and 0.202 ng/mL for atorvastatin and para-hydroxy atorvastatin in human plasma, respectively. The method was then successfully applied to the pharmacokinetic study of atorvastatin in healthy Chinese male subjects.
Liu
Dongyang
D
Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, Beijing 100730, China.
Jiang
Ji
J
Zhou
Hui
H
Hu
Pei
P
D000069059:Atorvastatin; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006538:Heptanoic Acids / Q000097:blood* / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D011758:Pyrroles / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
D006538:Heptanoic Acids; D011758:Pyrroles; D000069059:Atorvastatin