title: The expression pattern of PKCtheta in satellite cells of normal and regenerating muscle in the rat.
abstract: Protein kinase C (PKC) is a key enzyme in regulating a variety of cellular functions. PKCtheta is the most abundant PKC isoform expressed in skeletal muscle. However, the functional role of PKCtheta linked to muscle regeneration has not yet been identified. Using reverse transcription (RT)-PCR and immunofluorescence analysis, we investigated the expression patterns of PKCtheta in normal and regenerating tibialis anterior (TA) muscles in the rat. The amount of PKCtheta mRNA in the muscle increased from the 4th to 6th post-surgical day. Immunofluorescence revealed PKCtheta protein in quiescent satellite cells identified by c-Met. PKCtheta immunoreactivity was not observed in many proliferating satellite cells by labeling with BrdU in the regenerating muscle. At 4, 6 and 10 days postsurgery, PKCtheta immunoreactivity was observed in half the differentiating satellite cells labeling with myogenin. After 4 and 6 days, the localization of PKCtheta coincided with those of Pax7 and TGF-beta. Thus, PKCtheta may play an important role in inhibiting differentiation and maintaining the quiescent satellite cells in muscle regeneration.
authors.affiliation: Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medcicine, Kamigyo-ku, Kyoto.
authors.forename: Seiji
authors.initials: S
authors.lastname: Tokugawa
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Sakuma
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Miyuki
authors.initials: M
authors.lastname: Hirata
authors.forename: Ryo
authors.initials: R
authors.lastname: Oda
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Morisaki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yasuhara
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Kubo
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002454:Cell Differentiation; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000293:injuries / Q000502:physiology*; D018008:Myogenin / Q000378:metabolism; D051761:Paired Box Transcription Factors / Q000378:metabolism; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D000074121:Protein Kinase C-theta; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000201:enzymology*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology
chemical_list: D007527:Isoenzymes; D018008:Myogenin; C530821:PAX7 protein, rat; D051761:Paired Box Transcription Factors; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C489591:Prkcq protein, rat; D011493:Protein Kinase C; D000074121:Protein Kinase C-theta; D001973:Bromodeoxyuridine