Isolation and expansion of equine umbilical cord-derived matrix cells (EUCMCs).
Stem cells from extra-embryonic sources can be obtained by non-invasive procedures. We have standardized a method for the expansion of equine umbilical cord-derived matrix cells (EUCMCs) for potential therapy. EUCMCs were isolated from the umbilical cord of five mares immediately after delivery. For expansion, cells were grown in alpha-MEM and MSCBM. Moreover, to measure the effect of growth factor supplementation, epidermal growth factor (EGF) was added to alpha-MEM. alpha-MEM and MSCBM media performed similarly in terms of population doubling and CFU number value. EGF supplementation of alpha-MEM determined a significant increase of the population doubling value. EGF supplementation did not affect the adipogenic and chondrogenic differentiation while bone nodule sizes an increased with the osteogenic protocol. Both alpha-MEM and MSCBM can be used to cultivate EUCMCs. alpha-MEM supplemented with EGF might represent an advantage for EUCMCs expansion. The results could be useful in choosing the culture medium since alpha-MEM is more cost-effective than MSCBM.
Passeri
Simona
S
Department of Veterinary Anatomy, Biochemistry and Physiology, University of Pisa, Pisa, Italy.
Nocchi
Francesca
F
Lamanna
Roberta
R
Lapi
Simone
S
Miragliotta
Vincenzo
V
Giannessi
Elisabetta
E
Abramo
Francesca
F
Stornelli
Maria Rita
MR
Matarazzo
Micheletino
M
Plenteda
Daniele
D
Urciuoli
Patrizia
P
Scatena
Fabrizio
F
Coli
Alessandra
A
D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006736:Horses; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D014470:Umbilical Cord / Q000166:cytology*
D003470:Culture Media; D004815:Epidermal Growth Factor