Cytotoxicity of amphotericin B-incorporated polymeric micelles composed of poly(DL-lactide-co-glycolide)/dextran graft copolymer.
In this study, we prepared amphotericin B (AmpB)-encapsulated polymeric micelle of poly(DL-lactideco-glycolide) (PLGA) grafted-dextran (DexLG) copolymer for the cytotoxicity test. The average particle size of AmpB-encapsulated DexLG polymeric micelles was around 30 approximately 70 nm and their morphology showed spherical shapes. Since aggregation states of AmpB are related to intrinsic cytotoxicity, prevention of AmpB aggregation in aqueous solution will provide low cytotoxicity and increased antimicrobial activity for the infectious disease. At UV/VIS spectrum measurement, polymeric micelle prepared from methanol/water mixture (method B) showed a monomeric state of AmpB while polymeric micelle prepared from DMSO (method A) showed an aggregated state. During the hemolysis activity test, polymeric micelle from method B showed reduced hemolysis activity compared to AmpB itself and polymeric micelle from method A. These results indicated that AmpB-incorporated polymeric micelle prepared from methanol/water mixture has low cytotoxicity and favorable antimicrobial activity.
Bang
Je-Yong
JY
Faculty of Environment and Life Sciences, Seoul Women's University, Seoul 139-774, Korea.
Song
Chae-Eun
CE
Kim
Chan
C
Park
Woo-Dae
WD
Cho
Ki-Rae
KR
Kim
Pyoung-Il
PI
Lee
Sang-Rak
SR
Chung
Wan-Tae
WT
Choi
Ki-Choon
KC
D000666:Amphotericin B / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D003911:Dextrans; D004339:Drug Compounding; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D019344:Lactic Acid; D008823:Micelles; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013056:Spectrophotometry, Ultraviolet
D000935:Antifungal Agents; D003911:Dextrans; D008823:Micelles; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000666:Amphotericin B