Enhanced expression of CCL20 in human Helicobacter pylori-associated gastritis.
CC chemokine ligand 20 (CCL20) attracts CC chemokine receptor 6 (CCR6)-expressing cells. Using endoscopic biopsies taken from the gastric antrum of 42 subjects infected with H. pylori and 42 uninfected subjects, mucosal CCL20 mRNA and protein levels were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. CCL19 mRNA and protein levels, as well as CCL21 mRNA levels, were also measured. The CCL20 mRNA and protein levels were significantly elevated in H. pylori-positive patients and substantially decreased after successful eradication. CCL19 and CCL21 expression levels were comparable in the H. pylori-infected and the uninfected groups. The CCL20 concentrations correlated with the degree of chronic gastritis. Immunohistochemistry and the in vitro infection assay showed that CCL20 was principally produced by the gastric epithelium. CCR6-expressing cells, including CD45RO(+) memory T lymphocytes and fascin(+)-CD1a(+) immature dendritic cells, infiltrated close to the CCL20-expressing epithelial cells. The CCL20/CCR6 interaction may be involved in the development of H. pylori-associated gastritis.
Yoshida
Akira
A
Second Department of Internal Medicine, Nagasaki University School of Medicine, Sakamoto, Nagasaki 852-8501, Japan.
Isomoto
Hajime
H
Hisatsune
Junzo
J
Nakayama
Masaaki
M
Nakashima
Yujiro
Y
Matsushima
Kayoko
K
Mizuta
Yohei
Y
Hayashi
Tomayoshi
T
Yamaoka
Yoshio
Y
Azuma
Takeshi
T
Moss
Joel
J
Hirayama
Toshiya
T
Kohno
Shigeru
S
D000368:Aged; D000369:Aged, 80 and over; D002460:Cell Line; D054418:Chemokine CCL20 / Q000378:metabolism*; D004847:Epithelial Cells / Q000276:immunology; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology; D005756:Gastritis / Q000276:immunology* / Q000382:microbiology; D005786:Gene Expression Regulation; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged
D054418:Chemokine CCL20