title: Highly efficient Agrobacterium-mediated transformation of wheat via in planta inoculation.
abstract: This chapter details a reproducible method for the transformation of spring wheat using Agrobacterium tumefaciens via the direct inoculation of bacteria into immature seeds in planta as described in patent WO 00/63398 (1). Transformation efficiencies from 1 to 30% have been obtained and average efficiencies of at least 5% are routinely achieved. Regenerated plants are phenotypically normal with 30-50% of transformation events carrying introduced genes at single insertion sites, a higher rate than is typically reported for transgenic plants produced using biolistic transformation methods.
authors.affiliation: Biogemma, Clermont-Ferrand Cedex 2, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Risacher
authors.forename: Melanie
authors.initials: M
authors.lastname: Craze
authors.forename: Sarah
authors.initials: S
authors.lastname: Bowden
authors.forename: Wyatt
authors.initials: W
authors.lastname: Paul
authors.forename: Tina
authors.initials: T
authors.lastname: Barsby
mesh_terms: D000064:Acclimatization; D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D018014:Gene Transfer Techniques; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000382:microbiology; D012987:Soil; D013535:Suspensions; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic; D014908:Triticum / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology*
chemical_list: D012987:Soil; D013535:Suspensions