Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts.
A liquid chromatography-tandem mass spectrometric method has been developed for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate. The analytes were separated within 5 min using an anion exchange/reverse phase column. The lower limit of quantification for Glu, NAA and NAAG was found to be 5, 50 and 6 nM, respectively, with a signal-to-noise ratio of 5:1. Using this methodology the basal levels of Glu, NAA and NAAG could be measured consistently in in vitro superfusion samples from rat hippocampus. The assay was also used for measurement of the distribution of Glu, NAA and NAAG in different regions of the rat brain.
Shah
Ajit J
AJ
Neurosciences Centre of Excellence for Drug Discovery, GlaxoSmithKline Plc, New Frontiers Science Park, Third Avenue, Harlow, Essex CM19 5AW, UK. Ajit.J.shah@gsk.com
de la Flor
Raúl
R
Atkins
Alan
A
Slone-Murphy
Julia
J
Dawson
Lee A
LA
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004151:Dipeptides / Q000032:analysis*; D018698:Glutamic Acid / Q000032:analysis*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004151:Dipeptides; C027172:isospaglumic acid; D001224:Aspartic Acid; D018698:Glutamic Acid; C000179:N-acetylaspartate