title: Interactions of Taxol-producing endophytic fungus with its host (Taxus spp.) during Taxol accumulation.
abstract: Endophytic fungi (Fusarium mairei) culture broth (EFCB) was added to cell suspension cultures of Taxus cuspidata. After 5 days, cultures of T. cuspidata given 4 ml of EFCB produced a maximal yield of 6.11 mg/l paclitaxel, with a release ratio of 75%, 2- and 6.8-fold, respectively, greater than the controls. The active element in EFCB is an exopolysaccharide of approximately 79 kD. Endophytic fungi produced 0.19 mg/l of paclitaxel in its producing medium. However, when the supernatant of Taxus cell suspension cultures from day 20 was added to the paclitaxel-producing medium, the biomass of fungi decreased by 24% and the yield of paclitaxel by 45%. In a co-culture system of plant and fungus, the yield of paclitaxel (12.8 mg/l) was >2-fold higher than that in the EFCB-treatment system.
authors.affiliation: Key Laboratory of Industrial Biotechnology of the Ministry Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Li
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Tao
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D017239:Paclitaxel / Q000032:analysis / Q000096:biosynthesis*; D018515:Plant Leaves; D020946:Taxus / Q000254:growth & development / Q000382:microbiology*; D013997:Time Factors
chemical_list: D017239:Paclitaxel