SGNP: an essential Stress Granule/Nucleolar Protein potentially involved in 5.8s rRNA processing/transport.
BACKGROUND
Stress Granules (SG) are sites of accumulation of stalled initiation complexes that are induced following a variety of cellular insults. In a genetic screen for factors involved in protecting human myoblasts from acute oxidative stress, we identified a gene encoding a protein we designate SGNP (Stress Granule and Nucleolar Protein).


RESULTS
A gene-trap insertional mutagenesis screen produced one insertion that conferred resistance to sodium arsenite. RT-PCR/3' RACE was used to identify the endogenous gene expressed as a GFP-fusion transcript. SGNP is localized in both the cytoplasm and nucleolus and defines a non-nucleolar compartment containing 5.8S rRNA, a component of the 60S ribosomal subunit. Under oxidative stress, SGNP nucleolar localization decreases and it rapidly co-localizes with stress granules. The decrease in nucleolar SGNP following oxidative stress was accompanied by a large increase in nucleolar 5.8S rRNA. Knockdown of SGNP with shRNA increased global mRNA translation but induced growth arrest and cell death.


CONCLUSIONS
These results suggest that SGNP is an essential gene that may be involved in ribosomal biogenesis and translational control in response to oxidative stress.
Zhu
Chun-Hong
CH
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Kim
Jinyong
J
Shay
Jerry W
JW
Wright
Woodring E
WE
D021581:Active Transport, Cell Nucleus / Q000235:genetics*; D000818:Animals; D000911:Antibodies, Monoclonal; D017209:Apoptosis; D018053:Arsenites / Q000494:pharmacology; D001692:Biological Transport; D002466:Cell Nucleolus; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D016254:Mutagenesis, Insertional; D009154:Mutation; D032448:Myoblasts, Skeletal; D009687:Nuclear Proteins / Q000235:genetics*; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012335:RNA, Ribosomal / Q000378:metabolism*; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017421:Sequence Analysis; D017670:Sodium Compounds / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D018053:Arsenites; D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D016877:Oxidants; D012335:RNA, Ribosomal; D017670:Sodium Compounds; C017947:sodium arsenite