title: Isolation of herbicide-resistant 4-hydroxyphenylpyruvate dioxygenase from cultured Coptis japonica cells.
abstract: 4-Hydroxyphenylpyruvate dioxygenase (HPPD) catalyzes the formation of homogentisate from 4-hydroxyphenylpyruvate and O(2). In plants, HPPD has been identified as a molecular target for herbicides. We report the isolation and characterization of a cDNA encoding a HPPD from cultured Coptis japonica cells. Recombinant CjHPPD showed significantly higher half-maximum inhibitory concentration (IC(50)) values for the HPPD-inhibiting herbicide destosyl pyrazolate than other plant HPPDs.
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Liang
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Minami
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Sato
mesh_terms: D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001599:Berberine / Q000378:metabolism; D002478:Cells, Cultured; D039323:Coptis / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D015723:Gene Library; D053209:Herbicide Resistance; D006540:Herbicides / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D011720:Pyrazoles / Q000494:pharmacology
chemical_list: D006540:Herbicides; D011720:Pyrazoles; C534728:destosyl pyrazolate; D001599:Berberine; D015111:4-Hydroxyphenylpyruvate Dioxygenase