Two novel serum biomarkers for endometriosis screened by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and their change after laparoscopic removal of endometriosis.
OBJECTIVE
To explore novel endometriosis serum biomarkers by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).


METHODS
First, we aimed to discover the potential biomarkers of endometriosis by SELDI-TOF-MS. Second, blinded test was performed to characterize the effectiveness of the model by examining the sensitivity and specificity. Third, 29 postoperative patients with endometriosis were recruited to monitor the change of potential biomarkers after laparoscopic surgery.


METHODS
Collaborative investigation in an academic research environment.


METHODS
Fifty-nine patients with endometriosis, 31 patients without endometriosis, and 30 healthy volunteers.


METHODS
Blood serum of endometriosis and control group patients.


METHODS
Protein expression.


RESULTS
Two endometriosis-specific proteins were found in the preliminary screening study. Furthermore, the blinded test was performed and showed a sensitivity of 86.67% and a specificity of 96.77% of the markers for detecting endometriosis, which are significantly higher than those of CA-125 for distinguishing patients with endometriosis from patients without endometriosis. After surgery, the levels of these biomarker proteins decreased to levels comparable with those of patients without endometriosis.


CONCLUSIONS
We discovered the potential biomarkers of endometriosis and set up a diagnostic model with a sensitivity of 86.67% and a specificity of 96.77%, which is significantly higher than that of CA-125 for detecting endometriosis, The levels of these proteins decreased to levels comparable with those of patients without endometriosis.
Jing
Jianhong
J
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China. Electronic address: jijiho66@163.com.
Qiao
Yuhuan
Y
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China.
Suginami
Hiroshi
H
Department of Obstetrics and Gynecology, National Hospital Organization Kyoto Medical Center, Fushimi, Kyoto, Japan; Hiroshi Suginami, Takanohara Central Hospital, Ukyo, Nara, Japan.
Taniguchi
Fumiaki
F
Department of Obstetrics and Gynecology, National Hospital Organization Kyoto Medical Center, Fushimi, Kyoto, Japan.
Shi
Huirong
H
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China.
Wang
Xiuli
X
Center of Anal-Colorectal Surgery, the 150th Central Hospital of PLA, Luoyang, Henan Province, People's Republic of China.
D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000097:blood*; D018394:CA-125 Antigen / Q000097:blood; D004715:Endometriosis / Q000097:blood / Q000175:diagnosis* / Q000601:surgery; D005260:Female; D013509:Gynecologic Surgical Procedures / Q000379:methods*; D006801:Humans; D010535:Laparoscopy / Q000379:methods; D008403:Mass Screening; D008875:Middle Aged; D011379:Prognosis; D012680:Sensitivity and Specificity; D016037:Single-Blind Method; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014591:Uterine Diseases / Q000097:blood / Q000175:diagnosis* / Q000601:surgery; D055815:Young Adult
D015415:Biomarkers; D018394:CA-125 Antigen