Identification of the proteome of the midgut of silkworm, Bombyx mori L., by multidimensional liquid chromatography (MDLC) LTQ-Orbitrap MS.
The midgut is the digestive apparatus of the silkworm and its proteome was studied by using nano-LC (liquid chromatography) electrospray ionization MS/MS (tandem MS). MS data were analysed by using X!Tandem searching software using different parameters and validated by using the Poisson model. A total of 90 proteins were identified and 79 proteins were described for the first time. Among the new proteins, (i) 22 proteins were closely related to the digestive function of the midgut, including 11 proteins of digestive enzymes secreted by the epithelium, eight proteins of intestine wall muscle and mechanical digestion and three proteins of peritrophic membrane that could prevent the epithelium from being mechanically rubbed; (ii) 44 proteins were involved in metabolism of substance and energy; and (iii) 11 proteins were associated with signal transduction, substance transport and cell skeleton.
Yao
Hui-Peng
HP
College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China.
Xiang
Xing-Wei
XW
Chen
Lin
L
Guo
Ai-Qin
AQ
He
Fang-Qing
FQ
Lan
Li-Pan
LP
Lu
Xing-Meng
XM
Wu
Xiao-Feng
XF
D000818:Animals; D012831:Bombyx / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods; D004063:Digestion / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D019476:Insect Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D015233:Models, Statistical; D009130:Muscle, Smooth / Q000737:chemistry / Q000378:metabolism; D016012:Poisson Distribution; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction / Q000502:physiology; D012984:Software
D004798:Enzymes; D019476:Insect Proteins; D020543:Proteome