Periodic acceleration (pGz) acutely increases endothelial and neuronal nitric oxide synthase expression in endomyocardium of normal swine.
BACKGROUND
Periodic acceleration (pGz) is a non-invasive method of increasing pulsatile shear stress to the endothelium. pGz is achieved by the sinusoidal head to foot motion to the supine body. pGz increases endogenous production of nitric oxide in whole animal models and isolated perfused vessel preparations, and is cardioprotective when applied prior to, during and after ischemia reperfusion. In part, the protective effects of pGz are attributable to nitric oxide (NO). The purpose of this investigation was to determine whether pGz up-regulates NOS isoforms in the endomyocardium.


RESULTS
Fifteen swine weight 15-20 kg, were anesthetized, instrumented to measure hemodynamics and randomized. Ten animals received 1h of pGz at 180 cycles/min and Gz+/-3.9 m/s(2) [pGz] in addition to conventional ventilatory support and five served as time controls.


RESULTS
pGz produced a 2.3+/-0.4 and a 6.6+/-0.1 fold significant increase in eNOS and phosphorylated eNOS, 3.6+/-1.1 fold increase in nNOS, and no significant change in iNOS. pGz also produced a 2.4+/-0.3 and 3.9+/-0.2 folds significant increase in both total(t-Akt) and phosphorylated (p-Akt) Akt.


CONCLUSIONS
pGz is associated with an increase in both total and phosphorylated eNOS and nNOS protein expression in endomyocardium, and induced significant increase in total and phosphorylated-Akt. The data indicates that pGz is a novel method to induce eNOS and nNOS production in the endomyocardium. Therefore, pGz may serve as a powerful non-invasive intervention to activate the beneficial cardiac effects of endothelial and neuronal NOS.
Adams
Jose A
JA
Mount Sinai Medical Center, Division of Neonatology 3-BLUM, 4300 Alton Road, Miami Beach, FL 33140, USA. tony@msmc.com
Wu
Heng
H
Bassuk
Jorge A
JA
Arias
Jaqueline
J
Uryash
Arkady
A
Kurlansky
Paul
P
D000818:Animals; D001784:Blood Gas Analysis; D004699:Endocardium / Q000201:enzymology*; D006439:Hemodynamics; D008297:Male; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D033081:Shear Strength; D013552:Swine / Q000378:metabolism; D015854:Up-Regulation
D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III