title: Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes.
abstract: In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the first, only 10(-5), 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations of TE were used for 48 h to detect potential clastogenicity. In the second, MMC (0.38 mug ml(-1)) plus 10(-5), 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations of TE were used for 48 h to determine anticlastogenic effects. TE did not increase sister chromatid exchanges (SCEs) (except 10(-2) mul ml concentration) and chromosome aberrations (CAs) significantly compared with negative and solvent controls. However, it decreased the frequency of MMC induced chromosome aberrations. Decreasing was significant at 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations. On the other hand, TE significantly increased MMC-induced SCEs for all treatment groups compared with positive control.
authors.affiliation: Science Faculty, Department of Biology, Kahramanmaraş Sütcü Imam University, 46100, Kahramanmaraş, Türkiye.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Celik
authors.forename: Fatma
authors.initials: F
authors.lastname: Unal
authors.forename: Deniz
authors.initials: D
authors.lastname: Yüzbaşıoğlu
authors.forename: Serkan
authors.initials: S
authors.lastname: Yılmaz
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Aksoy
authors.forename: Sengül
authors.initials: S
authors.lastname: Karaman