title: Transcriptome analysis of the zebrafish mind bomb mutant.
abstract: Mind bomb (Mib) facilitates Notch signaling pathway by promoting the endocytosis of Notch ligand. The zebrafish mib ( ta52b ) mutant has a defect in its ubiquitin ligase activity which is necessary to inhibit the neurogenesis, resulting in a neuronal hyperplasia. Several genes regulated in the mib ( ta52b ) mutant have been well established, however, there were relatively few reports about the transcriptome profile. To identify the genes differentially expressed in the mib ( ta52b ) mutant, genome-wide analysis was performed using serial analysis of gene expression. Three hundred and thirty-five transcripts were identified whose expressions were significantly altered in the mib ( ta52b ) mutant as compared with the wild-type. Interestingly, it was suggested that the mib ( ta52b ) mutation may affect not only neurogenesis but also mesoderm development. These results provide new insights into Notch signaling pathway.
authors.affiliation: School of Life Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Junmo
authors.initials: J
authors.lastname: Hwang
authors.forename: Hyung-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Seok
authors.forename: Jun-Dae
authors.initials: JD
authors.lastname: Kim
authors.forename: Soonil
authors.initials: S
authors.lastname: Koun
authors.forename: So-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Jinseok
authors.initials: J
authors.lastname: Lee
authors.forename: Hyang Sook
authors.initials: HS
authors.lastname: Kim
authors.forename: Hyung-Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Kil Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Chang
authors.forename: Zae Young
authors.initials: ZY
authors.lastname: Ryoo
authors.forename: San Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Tae-Lin
authors.initials: TL
authors.lastname: Huh
authors.forename: Sanggyu
authors.initials: S
authors.lastname: Lee
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D055106:Genome-Wide Association Study; D017403:In Situ Hybridization; D008648:Mesoderm / Q000196:embryology; D009154:Mutation; D055495:Neurogenesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D051880:Receptors, Notch; D029961:Zebrafish Proteins; D044767:Ubiquitin-Protein Ligases; C471932:mib1 protein, zebrafish