title: The optimal immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella and its cross-immunity to Eimeria necatrix and Eimeria acervulina.
abstract: The immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella, including route, dose, time of immunization and age of primary immunization of chicken, was optimized. The stability and the cross-species protection of the vaccine were also analyzed. Efficacy of immunization was evaluated on the basis of oocyst decrease ratio, lesion score, body-weight gain and the anti-coccidial index (ACI). Chinese Yellow chickens were randomly distributed into corresponding groups (30/group). The challenged, unchallenged and vector control groups were designed. The results illustrated that 25 microg was the optimal dose and intramuscular injection was the most effective route to induce protective immunity. There were no significant differences of ACIs between boosting and non-boosting groups. Storage time and temperature had little effect on the immunizing efficacy of the vaccine. The vaccine could provide partial cross-protection against the challenge with E. necatrix and E. acervulina, but not with E. maxima.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Xiaokai
authors.initials: X
authors.lastname: Song
authors.forename: Lixin
authors.initials: L
authors.lastname: Xu
authors.forename: Ruofeng
authors.initials: R
authors.lastname: Yan
authors.forename: Xinmei
authors.initials: X
authors.lastname: Huang
authors.forename: Mohammed Ali A
authors.initials: MA
authors.lastname: Shah
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology; D002645:Chickens; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D016054:DNA, Protozoan / Q000276:immunology; D016786:Eimeria tenella / Q000276:immunology*; D017125:Genes, Protozoan / Q000235:genetics; D007109:Immunity; D007114:Immunization / Q000662:veterinary; D007115:Immunization Schedule; D007376:Interleukin-2 / Q000008:administration & dosage / Q000276:immunology*; D033661:Oocysts / Q000276:immunology; D011201:Poultry Diseases / Q000517:prevention & control*; D016052:Protozoan Vaccines / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D007376:Interleukin-2; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D019444:Vaccines, DNA