title: Identification of a novel trimethoprim resistance gene, dfrK, in a methicillin-resistant Staphylococcus aureus ST398 strain and its physical linkage to the tetracycline resistance gene tet(L).
abstract: A novel trimethoprim resistance gene, designated dfrK, was detected in close proximity to the tetracycline resistance gene tet(L) on the ca. 40-kb plasmid pKKS2187 in a porcine methicillin (meticillin)-resistant Staphylococcus aureus isolate of sequence type 398. The dfrK gene encodes a 163-amino-acid dihydrofolate reductase that differs from all so-far-known dihydrofolate reductases.
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institute, Neustadt-Mariensee, Germany.
authors.forename: Kristina
authors.initials: K
authors.lastname: Kadlec
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
mesh_terms: D000892:Anti-Infective Agents, Urinary / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016364:Reading Frames / Q000235:genetics; D013203:Staphylococcal Infections / Q000382:microbiology; D013753:Tetracycline Resistance / Q000235:genetics*; D014295:Trimethoprim / Q000494:pharmacology*; D014296:Trimethoprim Resistance / Q000235:genetics*
chemical_list: D000892:Anti-Infective Agents, Urinary; D001426:Bacterial Proteins; C539941:TetL protein, Staphyloccus aureus; D014295:Trimethoprim