title: Mammalian cell retention devices for stirred perfusion bioreactors.
abstract: Within the spectrum of current applications for cell culture technologies, efficient large-scale mammalian cell production processes are typically carried out in stirred fed-batch or perfusion bioreactors. The specific aspects of each individual process that can be considered when determining the method of choice are presented. A major challenge for perfusion reactor design and operation is the reliability of the cell retention device. Current retention systems include cross-flow membrane filters, spin-filters, inclined settlers, continuous centrifuges and ultrasonic separators. The relative merits and limitations of these technologies for cell retention and their suitability for large-scale perfusion are discussed.
authors.affiliation: Biotechnology Laboratory and Department of Chemical and Bio- Resource Engineering, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.
authors.forename: S M
authors.initials: SM
authors.lastname: Woodside
authors.forename: B D
authors.initials: BD
authors.lastname: Bowen
authors.forename: J M
authors.initials: JM
authors.lastname: Piret