Complete decontamination and regeneration of DNA purification silica columns.
Silica columns are among the most used DNA purification systems, allowing a good yield of high-quality nucleic acids without organic extractions. Silica column regeneration protocols reported up to now to remove DNA traces are time-consuming, and their effectiveness on genomic DNA has not been demonstrated. Here we report a very rapid regeneration procedure that ensures no DNA carryover, independent of its size, without impairing column efficiency. The method takes advantage of the improved DNA removal by low concentrations of Triton X-100.
Tagliavia
Marcello
M
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, 90128 Palermo, Italy. m.tagliavia@unipa.it
Nicosia
Aldo
A
Gianguzza
Fabrizio
F
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D004271:DNA, Fungal / Q000302:isolation & purification*; D016681:Genome, Fungal / Q000235:genetics; D017830:Octoxynol / Q000737:chemistry; D010316:Particle Size; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012822:Silicon Dioxide / Q000737:chemistry*
D004271:DNA, Fungal; D012822:Silicon Dioxide; D017830:Octoxynol