title: Abnormal expression of TIMP-2, SOD, vimentin and PAI proteins in cloned bovine placentae.
abstract: Cloned mammals suffer from high rates of placental abnormality and foetal loss during pregnancy. We previously used 2-D gel electrophoresis and mass spectrometry for global proteomic analysis of cloned and normal bovine placentae to identify differential protein expression patterns. Here, we used Western blot analysis to confirm the expression levels of several pregnancy-related proteins putatively identified as being differentially expressed in somatic cell nuclear transfer (SCNT) vs normal bovine placentae. The expression levels of tissue inhibitor of metalloproteinase-2 (TIMP-2), its downstream protein, matrix metalloproteinase-2 (MMP-2), superoxide dismutase (SOD), vimentin and plasminogen activator inhibitor-1 (PAI) were analysed in the placentae of SCNT cloned Korean native cattle that died immediately after birth and in normal placentae obtained by AI. Our results revealed that TIMP-2 and SOD were up-regulated in SCNT placenta compared with normal placenta, whereas MMP-2 levels were comparable in cloned and normal placentae, and vimentin and PAI were significantly down-regulated in SCNT compared with normal placentae. Our results suggest that key proteins of placental development are abnormally expressed in SCNT cloned bovine placentae, probably resulting in abnormal placental function and clonal mortality.
authors.affiliation: Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon, South Korea.
authors.forename: H-R
authors.initials: HR
authors.lastname: Kim
authors.forename: K
authors.initials: K
authors.lastname: Naruse
authors.forename: H-R
authors.initials: HR
authors.lastname: Lee
authors.forename: R-X
authors.initials: RX
authors.lastname: Han
authors.forename: C-S
authors.initials: CS
authors.lastname: Park
authors.forename: D-I
authors.initials: DI
authors.lastname: Jin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D002417:Cattle / Q000235:genetics*; D019976:Cloning, Organism / Q000379:methods; D005260:Female; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D053652:Nuclear Transfer Techniques; D010920:Placenta / Q000737:chemistry*; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000032:analysis*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis*; D014746:Vimentin / Q000032:analysis*
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D014746:Vimentin; D019716:Tissue Inhibitor of Metalloproteinase-2; D013482:Superoxide Dismutase; D020778:Matrix Metalloproteinase 2