title: Ladder-like amplification of the type I interferon gene cluster in the human osteosarcoma cell line MG63.
abstract: The organization of the type I interferon (IFN) gene cluster (9p21.3) was studied in a human osteosarcoma cell line (MG63). Array comparative genomic hybridization (aCGH) showed an amplification of approximately 6-fold which ended at both ends of the gene cluster with a deletion that extended throughout the 9p21.3 band. Spectral karyotyping (SKY) combined with fluorescence in-situ hybridization (FISH) identified an arrangement of the gene cluster in a ladder-like array of 5-7 'bands' spanning a single chromosome termed the 'IFN chromosome'. Chromosome painting revealed that the IFN chromosome is derived from components of chromosomes 4, 8 and 9. Labelling with centromeric probes demonstrated a ladder-like amplification of centromeric 4 and 9 sequences that co-localized with each other and a similar banding pattern of chromosome 4, as well as alternating with the IFN gene clusters. In contrast, centromere 8 was not detected on the IFN chromosome. One of the amplified centromeric 9 bands was identified as the functional centromere based on its location at the chromosome constriction and immunolocalization of the CENP-C protein. A model is presented for the generation of the IFN chromosome that involves breakage-fusion-bridge events.
authors.affiliation: Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, NY 14260, USA.
authors.forename: Narasimharao V
authors.initials: NV
authors.lastname: Marella
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Zeitz
authors.forename: Kishore S
authors.initials: KS
authors.lastname: Malyavantham
authors.forename: Artem
authors.initials: A
authors.lastname: Pliss
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Matsui
authors.forename: Sandra
authors.initials: S
authors.lastname: Goetze
authors.forename: Juergen
authors.initials: J
authors.lastname: Bode
authors.forename: Ivan
authors.initials: I
authors.lastname: Raska
authors.forename: Ronald
authors.initials: R
authors.lastname: Berezney
mesh_terms: D045744:Cell Line, Tumor; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics; D020223:Chromosome Painting; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007370:Interferon Type I / Q000235:genetics*; D007621:Karyotyping; D005810:Multigene Family / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D007370:Interferon Type I; C060905:centromere protein C