title: DNA status on thawed semen from fighting bull: a comparison between the SCD and the SCSA tests.
abstract: The assessment of sperm chromatin status is compulsory in a complete spermiogram. Here we applied the sperm chromatin structure assay (SCSA) and the sperm chromatin dispersion (SCD) test to assess the chromatin status of three fighting bulls. Cryopreserved semen (two straws/bull) were analysed by duplicate after thawing and after 6 h at 37 degrees C with and without oxidative stress (1 mm FE(2+)). Results (SCD: percentage of spermatozoa with halo; SCSA: SD-DFI, %DFI and HDS) were analysed for differences between bulls and treatments, sensitivity and specificity (receiver operating characteristic curves) and repeatability (repeatability coefficients as 2SD of duplicate differences).%DFI for the three bulls was below 2% at 0 h, indicating no risk for fertility according to previous reports. It increased slightly for two of the bulls after FE(2+) treatment (%DFI < 5%) and more pronouncedly for the other bull (C, %DFI approximately 10%), which merits further investigation. SCD rendered higher percentage of halos for bull C, but could not discriminate between samples with and without oxidizing treatment (AUC: 0.52). SCSA (%DFI) showed a high discriminating ability between treatments (AUC: 0.96). The repeatability coefficient was also higher for SCD (5.9) than for %DFI (1.8), indicating lower repeatability for SCD. Overall, %DFI might be the most useful parameter for assessing sperm chromatin on fighting bull. SCD might yield different information than SCSA, hence further research is warranted.
authors.affiliation: National Wildlife Research Institute (UCLM-CSIC-JCCM) and Institute of Regional Development, Section of Cynegetics and Cattle Resources, University of Castilla-La Mancha, Albacete, Spain. felipe.martinez@uclm.es
authors.forename: F
authors.initials: F
authors.lastname: Martínez-Pastor
authors.forename: M R
authors.initials: MR
authors.lastname: Fernández-Santos
authors.forename: M
authors.initials: M
authors.lastname: del Rocío Fernández-Santos
authors.forename: A E
authors.initials: AE
authors.lastname: Domínguez-Rebolledo
authors.forename: M C
authors.initials: MC
authors.lastname: Esteso
authors.forename: J J
authors.initials: JJ
authors.lastname: Garde
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002843:Chromatin / Q000648:ultrastructure*; D015925:Cryopreservation / Q000662:veterinary; D004247:DNA / Q000032:analysis*; D004249:DNA Damage; D005298:Fertility; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006358:Hot Temperature; D008297:Male; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry; D012662:Semen Preservation / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013094:Spermatozoa / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D002843:Chromatin; D005456:Fluorescent Dyes; D004247:DNA