title: Expression, crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Xanthomonas oryzae pv. oryzae.
abstract: Peptide deformylase (PDF) catalyzes the removal of the N-formyl group from the N-terminus of newly synthesized polypeptides; this process is crucial for cell survival. As it is an antibacterial drug target against Xanthomonas oryzae pv. oryzae (Xoo), PDF from Xoo was cloned, expressed, purified and crystallized. Native PDF crystals diffracted to 2.7 A resolution and belonged to the hexagonal space group P6(1)22, with unit-cell parameters a = b = 59.0, c = 266.3 A. One monomer is present in the asymmetric unit, with a corresponding crystal volume per protein weight of 3.50 A(3) Da(-1) and a solvent content of 64.9%.
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Phuong-Thuy Ho
authors.initials: PT
authors.lastname: Ngo
authors.forename: Jin-Kwang
authors.initials: JK
authors.lastname: Kim
authors.forename: Hyesoon
authors.initials: H
authors.lastname: Kim
authors.forename: Junho
authors.initials: J
authors.lastname: Jung
authors.forename: Yeh-Jin
authors.initials: YJ
authors.lastname: Ahn
authors.forename: Jeong-Gu
authors.initials: JG
authors.lastname: Kim
authors.forename: Byoung-Moo
authors.initials: BM
authors.lastname: Lee
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology; D014974:Xanthomonas / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D000581:Amidohydrolases; C084507:peptide deformylase