Mouse period 2 mRNA circadian oscillation is modulated by PTB-mediated rhythmic mRNA degradation.
Circadian mRNA oscillations are the main feature of core clock genes. Among them, period 2 is a key component in negative-feedback regulation, showing robust diurnal oscillations. Moreover, period 2 has been found to have a physiological role in the cell cycle or the tumor suppression. The present study reports that 3'-untranslated region (UTR)-dependent mRNA decay is involved in the regulation of circadian oscillation of period 2 mRNA. Within the mper2 3'UTR, both the CU-rich region and polypyrimidine tract-binding protein (PTB) are more responsible for mRNA stability and degradation kinetics than are other factors. Depletion of PTB with RNAi results in mper2 mRNA stabilization. During the circadian oscillations of mper2, cytoplasmic PTB showed a reciprocal expression profile compared with mper2 mRNA and its peak amplitude was increased when PTB was depleted. This report on the regulation of mper2 proposes that post-transcriptional mRNA decay mediated by PTB is a fine-tuned regulatory mechanism that includes dampening-down effects during circadian mRNA oscillations.
Woo
Kyung-Chul
KC
Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, South Korea.
Kim
Tae-Don
TD
Lee
Kyung-Ha
KH
Kim
Do-Yeon
DY
Kim
Wanil
W
Lee
Kyung-Yeol
KY
Kim
Kyong-Tai
KT
D020413:3' Untranslated Regions / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002940:Circadian Rhythm / Q000235:genetics*; D006224:Cricetinae; D015536:Down-Regulation; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D056950:Period Circadian Proteins; D038941:Polypyrimidine Tract-Binding Protein / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D020413:3' Untranslated Regions; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D038941:Polypyrimidine Tract-Binding Protein