Functional analysis of cotton small heat shock protein promoter region in response to abiotic stresses in tobacco using Agrobacterium-mediated transient assay.
The cotton (Gossypium arboreum) stress-related gene GHSP26 responds to dehydration. To elucidate its stress tolerant mechanism at the transcriptional level, we isolated and characterized the promoter region (PGHSP26, -2,831 bp) flanking the 5' GHSP26 coding region from the genomic DNA. A series of PGHSP26 deletion derivatives was created for the identification of the upstream region of the gene required for the promoter activity. Each deletion construct was analyzed by agrobacterium mediated transient transformation in tobacco leaves after treatment with abscissic acid (ABA), heavy metals and dehydration. Promoter fragments of 716 bp or longer showed two-fold or greater induction after each treatment. These findings further our understanding of the regulation of GHSP26 expression and provide a new drought-inducible promoter system in transgenic plants.
Zahur
Muzna
M
Center of Excellence in Molecular Biology, University of the Punjab, 87-Canal Bank Road, Thokar Niaz Baig, Lahore, Pakistan.
Maqbool
Asma
A
Irfan
Muhammad
M
Barozai
Muhammad Younas Khan
MY
Qaiser
Uzma
U
Rashid
Bushra
B
Husnain
Tayyab
T
Riazuddin
Shiekh
S
D000040:Abscisic Acid / Q000494:pharmacology; D001483:Base Sequence; D001681:Biological Assay / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017930:Genes, Reporter; D005966:Glucuronidase / Q000378:metabolism; D003368:Gossypium / Q000187:drug effects / Q000235:genetics*; D050888:Heat-Shock Proteins, Small / Q000235:genetics*; D019216:Metals, Heavy / Q000633:toxicity; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012231:Rhizobium / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000235:genetics*
D050888:Heat-Shock Proteins, Small; D019216:Metals, Heavy; D000040:Abscisic Acid; D005966:Glucuronidase