The mouse and human Liprin-alpha family of scaffolding proteins: genomic organization, expression profiling and regulation by alternative splicing.
In the nervous system the Liprin-alpha protein family plays an important role in the regulation of dendrite development, the targeting of photoreceptor axons, and the formation and structure of synapses. To gain a better understanding of Liprin-alpha regulation we have comparatively analyzed the genomic organization of the human and mouse Liprin-alpha genes, characterized the alternative exon use in human and mouse, and studied their expression in adult rodent tissues and brain regions. Our results show that Liprins-alpha1-4 share multiple properties in their genomic structure, exhibit an identical modular organization, and are highly conserved within certain structural domains, indicating strong evolutionary cohesion. We demonstrate that all Liprin-alpha genes are subject to alternative splicing, which is regulated in a developmental manner. Interestingly, regulation via alternative splicing is not conserved between isoforms and across species and represents a post-transcriptional mechanism to independently diversify the properties of the individual isoforms.
Zürner
Magdalena
M
Department of Neuropathology, University of Bonn, Bonn, Germany. magdalenazuerner@gmail.com
Schoch
Susanne
S
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D023061:Gene Order; D016678:Genome / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D048868:Adaptor Proteins, Signal Transducing; C094398:PPFIA1 protein, human; D020033:Protein Isoforms