title: Androgens in relationship to cardiovascular risk factors in the menopausal transition.
abstract: OBJECTIVE
  To establish the relationship between androgens and cardiovascular disease (CVD) risk factors in the menopausal transition.
  
  
  METHODS
  A total of 124 women were divided into four groups: 29 premenopausal (PreM), 35 women in the menopausal transition still menstruating (MTM), 29 women in the menopausal transition with 3-6 months amenorrhea (MTA), and 31 postmenopausal women (PostM). Levels of triglycerides, low density lipoprotein (LDL) cholesterol, high density lipoprotein (HDL) cholesterol, glucose and insulin were assayed in all samples and waist circumference was measured. In a subgroup of 83 women (19 PreM, 21 MTM, 28 MTA and 15 PostM), levels of total testosterone, androstenedione, dehydroepiandrosterone sulfate (DHEAS) and estradiol were determined. The free androgen index, Homeostasis Model Assessment (HOMA) index, Quantitative Insulin Sensitivity Check Index (QUICKI) and McAuley index, estradiol/total testosterone and triglyceride/HDL cholesterol ratios were calculated.
  
  
  RESULTS
  Androstenedione was higher in MTA vs. PostM women (p < 0.05); DHEAS was higher in PreM women vs. the other three groups (p < 0.05). Sex hormone binding globulin (SHBG) in MTM women was higher than in MTA women (p < 0.05); the free androgen index was lower in MTM women than in MTA and PostM women. SHBG and the free androgen index showed negative and positive correlations, respectively with waist circumference, insulin resistance and lipids. In a multiple regression analysis, considering waist circumference, neither free androgen index nor SHBG showed significant differences between groups. The waist circumference correlated only with SHBG (p = 0.022) and correlations between SHBG and insulin resistance markers continued to be significant, but relationships between SHBG and lipoproteins and all correlations found with free androgen index were lost.
  
  
  CONCLUSIONS
  An increment in the androgenic milieu that correlates with abdominal fat, insulin resistance and atherogenic lipoproteins becomes evident after the menopausal transition and suggests that evaluation of cardiovascular disease risk in these women should include androgens, considering that abdominal obesity is one of the main determinants of the relationship between androgenic parameters and cardiovascular risk factors.
authors.affiliation: Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: V R
authors.initials: VR
authors.lastname: Mesch
authors.forename: N O
authors.initials: NO
authors.lastname: Siseles
authors.forename: P N
authors.initials: PN
authors.lastname: Maidana
authors.forename: L E
authors.initials: LE
authors.lastname: Boero
authors.forename: F
authors.initials: F
authors.lastname: Sayegh
authors.forename: M
authors.initials: M
authors.lastname: Prada
authors.forename: M
authors.initials: M
authors.lastname: Royer
authors.forename: L
authors.initials: L
authors.lastname: Schreier
authors.forename: H J
authors.initials: HJ
authors.lastname: Benencia
authors.forename: G A
authors.initials: GA
authors.lastname: Berg
mesh_terms: D050153:Abdominal Fat; D000328:Adult; D000367:Age Factors; D000368:Aged; D000728:Androgens / Q000097:blood*; D000735:Androstenedione / Q000097:blood; D001118:Argentina / Q000453:epidemiology; D015992:Body Mass Index; D002318:Cardiovascular Diseases / Q000453:epidemiology / Q000378:metabolism*; D008076:Cholesterol, HDL / Q000097:blood; D019314:Dehydroepiandrosterone Sulfate / Q000097:blood; D004958:Estradiol / Q000097:blood; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008074:Lipoproteins / Q000097:blood*; D008593:Menopause / Q000378:metabolism*; D008875:Middle Aged; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood
chemical_list: D000728:Androgens; D008076:Cholesterol, HDL; D008074:Lipoproteins; D014280:Triglycerides; D000735:Androstenedione; D004958:Estradiol; D019314:Dehydroepiandrosterone Sulfate