title: Annexin A2 expression and phosphorylation are up-regulated in hepatocellular carcinoma.
abstract: Annexins (ANXs) constitute a family of Ca2+-dependent membrane-binding proteins; at least 20 of them have been described to date. Among these, Annexin A2 (ANXA2) has been revealed as a multi-functional protein in vitro. Its actual role in vivo, however, requires further investigation. We already reported that ANX-I (ANXA1) was up-regulated in hepatocellular carcinoma (HCC). The role of ANXA2 in various liver diseases including HCC remains obscure. In the present study, the protein and mRNA levels of ANXA2, as well as its localization, were determined for the normal human liver, chronic hepatitis liver, and non-tumorous and tumorous portions of HCC tissues. ANXA2 was rarely detected in either normal or chronic hepatitis liver tissues, whereas it was overexpressed at both the transcriptional and translational levels in tumorous and non-tumorous regions of HCC. In addition, in many cases, more ANXA2 was expressed in the tumorous portion than in the non-tumorous portion of HCC. The expression of ANXA2 was mainly localized in cancer cells, especially in poorly differentiated HCC. Furthermore, ANXA2 was tyrosine-phosphorylated in HCC. These data suggest that overexpression and tyrosine phosphorylation of ANXA2 play important roles in the malignant transformation process leading to HCC and are related to the histological grade of HCC.
authors.affiliation: Department of Gastroenterology and Neurology, Kagawa Medical University School of Medicine, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: Hamdy Saad
authors.initials: HS
authors.lastname: Mohammad
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kurokohchi
authors.forename: Hirohito
authors.initials: H
authors.lastname: Yoneyama
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tokuda
authors.forename: Asahiro
authors.initials: A
authors.lastname: Morishita
authors.forename: Gong
authors.initials: G
authors.lastname: Jian
authors.forename: Liu
authors.initials: L
authors.lastname: Shi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Murota
authors.forename: Joji
authors.initials: J
authors.lastname: Tani
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Kato
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Miyoshi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Deguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Himoto
authors.forename: Hisashi
authors.initials: H
authors.lastname: Usuki
authors.forename: Hisao
authors.initials: H
authors.lastname: Wakabayashi
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Izuishi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Iwama
authors.forename: Kazushi
authors.initials: K
authors.lastname: Deguchi
authors.forename: Naohito
authors.initials: N
authors.lastname: Uchida
authors.forename: Eman A
authors.initials: EA
authors.lastname: Sabet
authors.forename: Usama A
authors.initials: UA
authors.lastname: Arafa
authors.forename: Ali T A
authors.initials: AT
authors.lastname: Hassan
authors.forename: Adel A
authors.initials: AA
authors.lastname: El-Sayed
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Masaki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017306:Annexin A2 / Q000032:analysis* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D015152:Blotting, Northern; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008103:Liver Cirrhosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D008113:Liver Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D014443:Tyrosine; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C472822:ANXA2 protein, human; D017306:Annexin A2; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D014443:Tyrosine