title: Synthesis and preliminary biological evaluation of novel pyrazolo[1,5-a]pyrazin-4(5H)-one derivatives as potential agents against A549 lung cancer cells.
abstract: A series of novel pyrazolo[1,5-a]pyrazin-4(5H)-one derivatives were synthesized by the reaction of ethyl 3-aryl-1-(2-bromoethyl)-1H-pyrazole-5-carboxylate and amine in the general heating condition and microwave-assisted condition. The structures of the compounds were determined by IR, (1)H NMR and mass spectroscopy, in addition, representative single-crystal structures were characterized by using X-ray diffraction analysis. Preliminary biological evaluation showed that the compounds could inhibit the growth of A549 cells in dosage- and time-dependent manners. The study on structure-activity relationships showed that compounds with 4-chlorophenyl group at pyrazole moiety, such as 5-benzyl-2-(4-chlorophenyl)-6,7-dihydropyrazolo[1,5-a]pyrazin-4(5H)-one (3o) had much more inhibitory effects. Compound 3o was the most effective small molecule in inhibiting A549 cell growth and might perform its action through modulating autophagy.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Zhang
authors.forename: Chuan-Dong
authors.initials: CD
authors.lastname: Fan
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
authors.forename: Dong-Soo
authors.initials: DS
authors.lastname: Shin
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Dong
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Xie
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000627:therapeutic use / Q000633:toxicity; D045744:Cell Line, Tumor; D018360:Crystallography, X-Ray; D004249:DNA Damage; D015195:Drug Design; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008175:Lung Neoplasms / Q000188:drug therapy*; D015394:Molecular Structure; D011720:Pyrazoles / Q000138:chemical synthesis* / Q000627:therapeutic use / Q000633:toxicity*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D011720:Pyrazoles