17beta-Estradiol-induced remodeling of GABAergic axo-somatic synapses on estrogen receptor expressing neurons in the anteroventral periventricular nucleus of adult female rats.
Experimental data demonstrate that the nervous system is widely influenced by sex hormones and the brain is continuously shaped by the changing hormone milieu throughout the whole life. Earlier we demonstrated that on the effect of estradiol there is a cyclic synaptic remodeling, i.e. a transient decrease in the number of GABAergic axo-somatic synapses in the arcuate nucleus. By using preembedding estrogen receptor and postembedding GABA immunostaining, in the present paper we studied the specificity of this effect and we found that in the anteroventral periventricular nucleus (AvPv) of adult female rats 17beta-estradiol treatment does not affect all synapses and neurons. In contrast to the arcuate nucleus, hormonal treatment induces a significant increase of inhibitory axo-somatic synapses in the AvPv and we found selectivity at the level of the postsynaptic neurons, as well. We analyzed the hormone-induced synaptic remodeling in estrogen receptor alpha and beta immunoreactive and non-labeled cells and the change in synapse number was observed only in neurons which express estrogen beta receptor.
Kurunczi
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A
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center, H-6701 Szeged, Hungary.
Hoyk
Z
Z
Csakvari
E
E
Gyenes
A
A
Párducz
A
A
D000818:Animals; D007025:Anterior Hypothalamic Nucleus / Q000166:cytology / Q000187:drug effects*; D001369:Axons / Q000502:physiology; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D016253:Microscopy, Immunoelectron / Q000379:methods; D009473:Neuronal Plasticity / Q000187:drug effects*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010052:Ovariectomy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D013569:Synapses / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D004967:Estrogens; D011960:Receptors, Estrogen; D004958:Estradiol; D005680:gamma-Aminobutyric Acid