title: Trifluoromethyldiazirine: an effective photo-induced cross-linking probe for exploring amyloid formation.
abstract: The separative and analytical power of ion mobility spectrometry-mass spectrometry combined with photo-induced cross-linking of site-specifically incorporated trifluoromethyldiazirine provides a powerful approach towards structural characterisation of amyloid fibrils.
authors.affiliation: Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, UK LS2 9JT.
authors.forename: David P
authors.initials: DP
authors.lastname: Smith
authors.forename: Jon
authors.initials: J
authors.lastname: Anderson
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Plante
authors.forename: Alison E
authors.initials: AE
authors.lastname: Ashcroft
authors.forename: Sheena E
authors.initials: SE
authors.lastname: Radford
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Wilson
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Parker
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D001389:Azirines / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D008697:Methane / Q000031:analogs & derivatives / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D017434:Protein Structure, Tertiary; D021241:Spectrometry, Mass, Electrospray Ionization; D014466:Ultraviolet Rays
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D001389:Azirines; D003432:Cross-Linking Reagents; D010455:Peptides; C543572:trifluoromethyldiazirine-phenylalanine; C030011:carbene; D010649:Phenylalanine; D008697:Methane