Lack of chemopreventive activity of Agaricus blazei mushroom on the development of 1,2-dimethylhydrazine-induced colonic aberrant crypt foci in rats.
Chemopreventive and immunomodulatory potential of methanolic (MET) and dichloromethanic (DCl) extracts of Agaricus blazei mushroom were investigated in the postinitiation stage of colon carcinogenesis in male Wistar rats. Animals were initiated with 1,2-dimethylhydrazine (DMH) and treated i.g. with DCl or MET extracts. After 9 wk, animals were sacrificed for evaluation of aberrant crypt foci (ACF) development, crypt cellular proliferation, preneoplastic liver lesions (GST-P), proliferative response of spleen cells to mitogen, and natural killer activity. Administration of DCl extracts did not suppress DMH-induced colonic ACF nor did it affect the crypt multiplicity, but the highest dose of MET significantly reduced the development of preneoplastic lesions in the colon and liver. Lymphoproliferative response was slightly decreased in the initiated control group, which was restored by treatment with MET. No toxicity from DCl and MET extracts was observed (groups MET and DCl).
Ribeiro-Santos
Giovanna
G
Department of Microbiology and Immunology, Institute of Biosciences, Sao Paulo State University-UNESP, Botucatu, SP, Brazil.
Barbisan
Luis Fernando
LF
Lopes
Fabiane Catanho
FC
Spinardi-Barbisan
Ana Lucia Tozzi
AL
da Eira
Augusto Ferreira
AF
Kaneno
Ramon
R
D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D000364:Agaricus; D000818:Animals; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D051549:Glutathione S-Transferase pi / Q000032:analysis; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D011230:Precancerous Conditions / Q000139:chemically induced / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar
D051549:Glutathione S-Transferase pi; C496558:Gstp1 protein, rat; D019813:1,2-Dimethylhydrazine