title: The first detection of Cryptosporidium deer-like genotype in cattle in Japan.
abstract: The general perception is that cattle are major reservoirs for Cryptosporidium parvum infections in humans and that C. parvum is a major cause of diarrhea and production loss in cattle. Adult cattle may play an important role as cryptic carrier of the infection. Cryptosporidium spp. in asymptomatic adult dairy cattle from some farms around Osaki area, Miyagi prefecture, Japan, was examined on a field visit during August, 2007, by polymerase chain reaction techniques for detection, genotyping, and subtyping. Cryptosporidium oocysts were detected in the feces of five out of 50 animals. Of the five Cryptosporidium-positive specimens available for molecular analysis, C. parvum was identified in three specimens, Cryptosporidium deer-like genotype in one, and Cryptosporidium andersoni in one specimen. Amplification of Cpgp60 from C. andersoni and Cryptosporidium deer-like genotype samples revealed that these samples have light concurrent C. parvum infection. Sequence analysis of the 60-kDa glycoprotein gene indicated that all C. parvum samples are IIa subtype. Detection of Cryptosporidium deer-like genotype is geographically unique in Japan. The genetic diversity of Cryptosporidium in dairy cattle in Japan may be much greater than that reported before.
authors.affiliation: Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogita, Naruko-onshen, Osaki, Miyagi 989-6711, Japan.
authors.forename: Said
authors.initials: S
authors.lastname: Amer
authors.forename: Hajime
authors.initials: H
authors.lastname: Honma
authors.forename: Makoto
authors.initials: M
authors.lastname: Ikarashi
authors.forename: Ryu
authors.initials: R
authors.lastname: Oishi
authors.forename: Mikiko
authors.initials: M
authors.lastname: Endo
authors.forename: Kenichi
authors.initials: K
authors.lastname: Otawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakai
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D003612:Dairying; D003670:Deer; D005243:Feces / Q000469:parasitology; D020459:Genes, rRNA; D005838:Genotype; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S