title: Importance of translation-replication balance for efficient replication by the self-encoded replicase.
abstract: In all living systems, the genetic information is replicated by the self-encoded replicase (Rep); this can be said to be a self-encoding system. Recently, we constructed a self-encoding system in liposomes as an artificial cell model, consisting of a reconstituted translation system and an RNA encoding the catalytic subunit of Qbeta Rep and the RNA was replicated by the self-encoded Rep produced by the translation reaction. In this system, both the ribosome (Rib) and Rep bind to the same RNA for translation and replication, respectively. Thus, there could be a dilemma: effective RNA replication requires high levels of Rep translation, but excessive translation in turn inhibits replication. Herein, we actually observed the competition between the Rib and Rep, and evaluated the effect for RNA replication by constructing a kinetic model that quantitatively explained the behavior of the self-encoding system. Both the experimental and theoretical results consistently indicated that the balance between translation and replication is critical for an efficient self-encoded system, and we determined the optimum balance.
authors.affiliation: Department of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osaka University, Suita, Osaka, Japan.
authors.forename: Norikazu
authors.initials: N
authors.lastname: Ichihashi
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Matsuura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kita
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Hosoda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sunami
authors.forename: Koji
authors.initials: K
authors.lastname: Tsukada
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yomo
mesh_terms: D000465:Algorithms; D007700:Kinetics; D008956:Models, Chemical; D014176:Protein Biosynthesis; D011777:Q beta Replicase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D016372:RNA, Antisense / Q000235:genetics; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism
chemical_list: D016372:RNA, Antisense; D012313:RNA; D011777:Q beta Replicase