Activation of a Nodal-independent signaling pathway by Cripto-1 mutants with impaired activation of a Nodal-dependent signaling pathway.
Cripto-1, a co-receptor for Nodal, can activate Nodal-dependent and Nodal-independent signaling pathways. In this study we have investigated whether Cripto-1 mutants, that fail to activate a Nodal-dependent signaling pathway, are capable to activate a Nodal-independent signaling pathway in mammary epithelial cells. Cripto-1 mutants expressed in EpH4 mouse mammary epithelial cells are fully functional in regard to activation of a Nodal-independent signaling pathway, leading to phosphorylation of mitogen-activated protein kinase (MAPK) and Akt and to enhanced proliferation and motility of these cells, suggesting that Cripto-1 mutants with impaired Nodal signaling are still active in a Nodal-independent signaling pathway.
Bianco
Caterina
C
Mammary Biology and Tumorigenesis Laboratory, NCI/NIH, 37 Convent Drive, Building 37 room 1112, Bethesda, MD 20892, USA. biancoc@mail.nih.gov
Mysliwiec
Margaret
M
Watanabe
Kazuhide
K
Mancino
Mario
M
Nagaoka
Tadahiro
T
Gonzales
Monica
M
Salomon
David S
DS
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D053673:Glypicans / Q000378:metabolism; D008321:Mammary Glands, Animal; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D055457:Nodal Protein / Q000378:metabolism*; D015398:Signal Transduction
D053673:Glypicans; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D055457:Nodal Protein; C526008:Nodal protein, mouse; C489450:Tdgf1 protein, mouse; D004815:Epidermal Growth Factor