title: Diversity and similarity of microbial communities in petroleum crude oils produced in Asia.
abstract: To understand microbial communities in petroleum crude oils, we precipitated DNA using high concentrations of 2,2,4-trimethylpentane (isooctane) and purified. Samples of DNA from five crude oils, (Middle East, 3; China, 1; and Japan, 1) were characterized based upon their 16S rRNA gene sequences after PCR amplification and the construction of clone libraries. We detected 48 eubacterial species, one cyanobacterium, and one archaeon in total. The microbial constituents were diverse in the DNA samples. Most of the bacteria affiliated with the sequences of the three oils from the Middle East comprised similar mesophilic species. Acinetobacter, Propionibacterium, Sphingobium and a Bacillales were common. In contrast, the bacterial communities in Japanese and Chinese samples were unique. Thermophilic Petrotoga-like bacteria (11%) and several anaerobic-thermophilic Clostridia- and Synergistetes-like bacteria (20%) were detected in the Chinese sample. Different thermophiles (12%) and Clostridia (2%) were detected in the Japanese sample.
authors.affiliation: National Food Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Yamane
authors.forename: Hideaki
authors.initials: H
authors.lastname: Maki
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakayama
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakajima
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Nomura
authors.forename: Hiroo
authors.initials: H
authors.lastname: Uchiyama
authors.forename: Motomitsu
authors.initials: M
authors.lastname: Kitaoka
mesh_terms: D001105:Archaea / Q000235:genetics / Q000302:isolation & purification; D001208:Asia; D011232:Chemical Precipitation; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics / Q000302:isolation & purification; D019641:DNA, Archaeal / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005051:Eubacterium / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D009795:Octanes / Q000737:chemistry; D010578:Petroleum / Q000191:economics / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D009795:Octanes; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; C045798:2,2,4-trimethylpentane