Highly efficient Agrobacterium-mediated transformation of wheat via in planta inoculation.
This chapter details a reproducible method for the transformation of spring wheat using Agrobacterium tumefaciens via the direct inoculation of bacteria into immature seeds in planta as described in patent WO 00/63398 (1). Transformation efficiencies from 1 to 30% have been obtained and average efficiencies of at least 5% are routinely achieved. Regenerated plants are phenotypically normal with 30-50% of transformation events carrying introduced genes at single insertion sites, a higher rate than is typically reported for transgenic plants produced using biolistic transformation methods.
Risacher
Thierry
T
Biogemma, Clermont-Ferrand Cedex 2, France.
Craze
Melanie
M
Bowden
Sarah
S
Paul
Wyatt
W
Barsby
Tina
T
D000064:Acclimatization; D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D018014:Gene Transfer Techniques; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000382:microbiology; D012987:Soil; D013535:Suspensions; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic; D014908:Triticum / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology*
D012987:Soil; D013535:Suspensions