Prenatal administration of granulocyte-macrophage colony-stimulating factor increases mesenchymal vascular endothelial growth factor expression and maturation in fetal rat lung.
The aim of this study was to determine influence of prenatal granulocyte-macrophage colony-stimulating factor (GM-CSF) administration on lung growth, maturation, and vascular endothelial growth factor (VEGF) expression. Twenty Wistar rats received sterile saline (1 mL) or recombinant human GM-CSF (50 micro g/kg) on day 16 of pregnancy. Rats were sacrificed on days 18 and 20 of gestation. H-score for VEGF was calculated immunohistochemically. Alveolar VEGF expression on days 18 and 20 of gestation was significantly higher in the GM-CSF group (P < .01). Increase in VEGF with prenatal GM-CSF administration indicates that GM-CSF may stimulate lung growth and maturation and may be protective against lung disease due to prematurity.
Yuksel
Hasan
H
Department of Pediatric Allergy and Pulmonology, Celal Bayar University, Manisa, Turkey. hyukselefe@hotmail.com
Yilmaz
Ozge
O
Baytur
Yesim Bulbul
YB
Ozbilgin
Kemal
K
D000818:Animals; D005333:Fetus; D015870:Gene Expression / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D008648:Mesoderm / Q000196:embryology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D016178:Granulocyte-Macrophage Colony-Stimulating Factor