title: Whole-genome mutational biases in bacteria.
abstract: A fundamental biological question is what forces shape the guanine plus cytosine (GC) content of genomes. We studied the specificity and rate of different mutational biases in real time in the bacterium Salmonella typhimurium under conditions of strongly reduced selection and in the absence of the major DNA repair systems involved in repairing common spontaneous mutations caused by oxidized and deaminated DNA bases. The mutational spectrum was determined by whole-genome sequencing of two S. typhimurium mutants that were serially passaged for 5,000 generations. Analysis of 943 identified base pair substitutions showed that 91% were GC-to-TA transversions and 7% were GC-to-AT transitions, commonly associated with 8-oxoG- and deamination-induced damages, respectively. Other types of base pair substitutions constituted the remaining 2% of the mutations. With regard to mutational biases, there was a significant increase in C-to-T transitions on the nontranscribed strand, and for highly expressed genes, C/G-to-T mutations were more common than expected; however, no significant mutational bias with regard to leading and lagging strands of replication or chromosome position were found. These results suggest that, based on the experimentally determined mutational rates and specificities, a bacterial genome lacking the relevant DNA repair systems could, as a consequence of these underlying mutational biases, very rapidly reduce its GC content.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, S-75123 Uppsala, Sweden.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Lind
authors.forename: Dan I
authors.initials: DI
authors.lastname: Andersson
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D020029:Base Pairing / Q000235:genetics; D015982:Bias; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D010802:Phylogeny; D012293:Rifampin / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D012293:Rifampin