title: Preparation and characterization of bisphenol A-cationized bovine serum albumin.
abstract: To detect BPA residue in the environment quickly by immune method, preparation of high quality antibody against BPA was the most critical step of project. Based on Mannich-type reaction and EDC method, we produced BPA-cBSA and BHPVA-BSA against BPA and the conjugates were characterized by UV-Vis and FT-IR spectroscopy. Compared with BSA, cBSA as carrier protein could improve the efficiency of coupling and significantly enhance the immune response against BPA. The mole coupling ratio of BPA-cBSA (8:1) was greater than that of BHPVA-BSA (5:1). We also observed that the similar sensitivity of antisera against BPA between the BPA-cBSA group and BHPVA-BSA group.
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Youxiang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zou
authors.forename: Jixing
authors.initials: J
authors.lastname: Wang
authors.forename: Fusheng
authors.initials: F
authors.lastname: Chen
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Gao
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D001559:Benzhydryl Compounds; D002412:Cations / Q000737:chemistry; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry*; D013053:Spectrophotometry; D000098826:Bisphenol A Compounds
chemical_list: D000906:Antibodies; D001559:Benzhydryl Compounds; D002412:Cations; D010636:Phenols; D012710:Serum Albumin, Bovine; C006780:bisphenol A; D000098826:Bisphenol A Compounds