Thickness of receptor and post-receptor retinal layers in patients with retinitis pigmentosa measured with frequency-domain optical coherence tomography.
OBJECTIVE
To better understand the effects of retinitis pigmentosa (RP) on post-receptor anatomy, the thicknesses of the receptor, inner nuclear, retinal ganglion cell (RGC), and retinal nerve fiber layers (RNFL) were measured with frequency-domain optical coherence tomography (fdOCT).


METHODS
FdOCT scans were obtained from the horizontal midline in 30 patients with RP and 23 control subjects of comparable age. Raw images were exported and the thicknesses of photoreceptor/RPE, inner nuclear, RGC plus inner plexiform, and nerve fiber layers were measured with a manual segmentation procedure aided by a computer program. The RNFL thickness was also measured in 20 controls and 25 patients using circular peripapillary fdOCT scans.


RESULTS
Results from controls were consistent with known anatomy. In patients with RP, the pattern of photoreceptor loss with eccentricity was consistent with the field constriction characteristic of RP. INL and RGC layer measures were comparable to normal subjects, although some patients showed slightly thicker RGC layers. However, RNFL layer thickness was significantly greater than normal; a majority of patients showed a thicker RFNL on both horizontal midline scans and peripapillary scans.


CONCLUSIONS
To make optimal use of OCT RNFL thickness as a measure of the integrity of RGCs in patients with RP, a better understanding of the causes of the thickening seen in the majority of the patients is needed. As the RGC layer thickness can be measured with fdOCT, RGC layer thickness may turn out to be a more direct and valid indicator of the presence of RGCs in patients with RP.
Hood
Donald C
DC
Department of Psychology, Columbia University, New York, NY 10027, USA. dch3@columbia.edu
Lin
Christine E
CE
Lazow
Margot A
MA
Locke
Kirsten G
KG
Zhang
Xian
X
Birch
David G
DG
D000293:Adolescent; D000328:Adult; D000368:Aged; D001837:Body Weights and Measures; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009412:Nerve Fibers / Q000473:pathology*; D020419:Photoreceptor Cells, Vertebrate / Q000473:pathology*; D012165:Retinal Ganglion Cells / Q000473:pathology*; D055351:Retinal Neurons / Q000473:pathology*; D012174:Retinitis Pigmentosa / Q000175:diagnosis*; D041623:Tomography, Optical Coherence / Q000379:methods; D055815:Young Adult