Expression of chlorophyllase is not induced during autumnal yellowing in Ginkgo biloba.
Autumnal tints are one of the most fascinating natural phenomena, but the molecular mechanism of chlorophyll (Chl-)degradation in deciduous trees has not been fully understood. In this study, from the leaves of Ginkgo biloba, chlorophyllase-homologous GbCLH was cloned by RT-PCR with degenerated primers. The expression of GbCLH in different yellowing stages was analyzed by Northern hybridization. The expression level of GbCLH was highest in green leaves and significantly declined during the process of leaf yellowing. These results suggested that GbCLH should be involved in chlorophyll-homeostasis in Ginkgo biloba.
Tang
Lei
L
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
Okazawa
Atsushi
A
Itoh
Yoshiko
Y
Fukusaki
Ei-ichiro
E
Kobayashi
Akio
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology; D020441:Ginkgo biloba / Q000201:enzymology* / Q000502:physiology; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000201:enzymology / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons
D018076:DNA, Complementary; D018744:DNA, Plant; D011994:Recombinant Proteins; D002734:Chlorophyll; D002265:Carboxylic Ester Hydrolases; C019875:chlorophyllase