Barley transformation using biolistic techniques.
Microprojectile bombardment or biolistic techniques have been widely used for cereal transformation. These methods rely on the acceleration of gold particles, coated with plasmid DNA, into plant cells as a method of directly introducing the DNA. The first report of the generation of fertile, transgenic barley plants used biolistic techniques. However, more recently Agrobacterium-mediated transformation has been adopted as the method of choice for most cereals including barley. Biolistic procedures are still important for some barley transformation applications and also provide transient test systems for the rapid checking of constructs. This chapter describes methods for the transformation of barley using biolistic procedures and also highlights the use of the technology in transient assays.
Harwood
Wendy A
WA
Department of Crop Genetics, John Innes Centre, Colney, Norwich, UK.
Smedley
Mark A
MA
D019470:Biolistics / Q000379:methods*; D003470:Culture Media; D018744:DNA, Plant / Q000378:metabolism; D006046:Gold / Q000378:metabolism; D001467:Hordeum / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D009995:Osmosis; D030821:Plants, Genetically Modified; D012639:Seeds / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
D003470:Culture Media; D018744:DNA, Plant; D006046:Gold; D013095:Spermidine