Effects of Coccoloba uvifera L. on UV-stimulated melanocytes.
BACKGROUND
Exposure to ultraviolet (UV) radiation induces generation of reactive oxygen species, production of proinflammatory cytokines and melanocyte-stimulating hormone (MSH) as well as increase in tyrosinase activity. The potential photoprotective effects of Coccoloba uvifera extract (CUE) were evaluated in UV-stimulated melanocytes.


METHODS
Human epidermal melanocytes were used as an in vitro model to evaluate the effects of CUE on the production interleukin-1alpha (IL-1alpha), tumor necrosis factor alpha (TNF-alpha), and alpha-MSH under basal and UV-stimulated conditions. Antioxidant and anti-tyrosinase activities were also evaluated in membrane lipid peroxidation and mushroom tyrosinase assay, respectively.


RESULTS
Coccoloba uvifera L. showed antioxidant and anti-tyrosinase activities and also inhibited the production of IL-1alpha, TNF-alpha and alpha-MSH in melanocytes subjected to UV radiation (P<0.01). Moreover, CUE inhibited the activity of tyrosine kinase in cell culture under basal and UV radiation conditions (P<0.001), corroborating the findings of the mushroom tyrosinase assay.


CONCLUSIONS
This study supports the photoprotective potential of CUE.
Silveira
Jéssica Eleonora Pedroso Sanches
JE
Department of Pharmacology, São Paulo State University, Botucatu, Brazil.
Pereda
Maria del Carmen Velazquez
Mdel C
Eberlin
Samara
S
Dieamant
Gustavo Campos
GC
Di Stasi
Luiz Claudio
LC
D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism; D008544:Melanocytes / Q000187:drug effects* / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D019664:Polygonaceae / Q000737:chemistry*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D000975:Antioxidants; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009074:Melanocyte-Stimulating Hormones; D014442:Monophenol Monooxygenase; D011505:Protein-Tyrosine Kinases