p73 expression and its clinical significance in colorectal cancer.
OBJECTIVE
To explore p73 mRNA and p73 protein expression and their clinical significance in colorectal cancer.


METHODS
p73 mRNA and p73 protein expression were detected using hybridization in situ and immunohistochemical method in cancerous tissue from 60 patients of colorectal cancer and in paracancerous tissue from 23 patients among the corresponding 60 patients of colorectal cancer. Quantitative analysis was performed using Smartscape image analysis system.


RESULTS
Compared with paracancerous tissue, p73 mRNA and p73 protein expression in cancerous tissue was increased with a statistically significant difference (P < 0.01) and was associated with differentiation level and lymphatic metastasis.


CONCLUSIONS
High expression of p73 mRNA and p73 protein in colorectal cancer may be involved in the progression of colorectal cancer and p73 mRNA and p73 protein may serve as a potential index to predict differentiation level and prognosis of colorectal cancer.
Su
X-L
XL
Clinical Medical Research Centre, Inner Mongolia Medical College Affiliated Hospital, Hohhot, China. xlsu@hotmail.com
Ouyang
X-H
XH
Yan
M-R
MR
Liu
G-R
GR
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins