Staphylococcal toxic shock syndrome toxin-1 induces the translocation and secretion of high mobility group-1 protein from both activated T cells and monocytes.
High mobility group box-1 (HMGB-1) is a DNA-binding protein secreted by activated monocytes and has been identified as a key late mediator of endotoxic shock. We investigated the regulation of HMGB-1 in human peripheral blood mononuclear cells (PBMCs) following stimulation with the staphylococcal superantigen, toxic shock syndrome toxin-1 (TSST-1), and found that TSST-1, like LPS, induced the secretion of HMGB-1 from human PBMC. However, unlike monocyte-driven sepsis caused by endotoxin, translocation and secretion of HMGB-1 mediated by TSST-1 was dependent on the presence of both activated T cells and monocytes. Furthermore, we show that nuclear HMGB-1 is released from TSST-1 stimulated T cells. This finding presents a basis for investigating the potential of targeting HMGB-1 for the treatment of toxic shock syndrome, and provides further insight on the role of HMGB-1 in the cross-talk between activated monocytes and T cells.
Kalyan
Shirin
S
Vancouver Hospital & Health Sciences, Diamond Health Care Centre, Department of Medicine, Vancouver, BC, Canada V5Z1M9.
Chow
Anthony W
AW
D021581:Active Transport, Cell Nucleus; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology*; D024243:HMGB1 Protein / Q000378:metabolism*; D006801:Humans; D008213:Lymphocyte Activation; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D018089:Superantigens / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D001427:Bacterial Toxins; D004768:Enterotoxins; D024243:HMGB1 Protein; D011994:Recombinant Proteins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal