title: Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides.
abstract: Crystals of recombinant NovN, an O-carbamoyltransferase from Streptomyces spheroides, were grown by vapour diffusion. The protein crystallized in two different crystal forms. Crystal form I belonged to space group C2 and native data were collected to 2.9 A resolution in-house. Crystal form II had I-centred orthorhombic symmetry and native data were recorded to a resolution of 2.3 A at a synchrotron. NovN catalyses the final step in the biosynthesis of the aminocoumarin antibiotic novobiocin that targets the essential bacterial enzyme DNA gyrase.
authors.affiliation: Department of Biological Chemistry, John Innes Centre, Norwich NR4 7UH, England.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Gómez García
authors.forename: Caren L
authors.initials: CL
authors.lastname: Freel Meyers
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Walsh
authors.forename: David M
authors.initials: DM
authors.lastname: Lawson
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D019878:Carboxyl and Carbamoyl Transferases / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D009675:Novobiocin / Q000096:biosynthesis*; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D009675:Novobiocin; D019878:Carboxyl and Carbamoyl Transferases