A simple and fast method for cell recovery and DNA content analysis from various mouse tissues by flow cytometry.
Cell division in tissues can be investigated in various ways. We present here a method for improving cell recovery and cell cycle analysis for a wide range of mouse tissues. This strategy combines a cell isolation procedure for various mouse tissues based on intracardiac perfusion and subsequent treatment followed by flow cytometry. This easy and reproducible method allows a rapid analysis of nuclear DNA content, providing an estimate of the cell number at different phases of the cell cycle. This combined procedure could also be used for the isolation of specific cell subpopulations from different mouse tissues by fluorescence activated cell sorting.
Foiry
Laurent
L
Inserm U781, Clinique M. Lamy, Hôpital Necker Enfants Malades, 149 rue de Sèvres, 75015, Paris, France.
Mégret
Jérôme
J
Junien
Claudine
C
Gourdon
Geneviève
G