[Optimization of extraction procedure of psammosilene tunicoides by uniform design with pharmacodynamic index].
OBJECTIVE
For anti-inflammation, relieving pain and decreasing acute toxicity, the best condition for extracting procedure of Psammosilene tunicoides was chosen. And the relationships between several solvent extracts and pharmacodynamic index were studied.


METHODS
Uniform design with multi-targets was used in the optimization process, and the evaluate index were the results of anti-inflammation, relieving pain and LD50 were employed as the evaluating indexes. At the same time, the contents of the extracts, water extract, acetic ester extract and ether extract were determined.


RESULTS
The best condition is that the medicinal material is optimized with 8 times of water (pH 6-7) and 2 h of each time. The best condition is satisfactory by test verification. And the pharmacodynamic actions of anti-inflammation, relieving pain and LD, were not obviously related to the dry extracts, water extract, acetic ester extract and ether extract.


CONCLUSIONS
Uniform design method with pharmacodynamic index has the merit of being relaated to the function and symptom directly. The extracted twice technology condition optimized is stable, feasible and suitable for industry production. And it is not suitable to take the solvent extracts as quality control index related to the function and symptom.
Huang
Chun-qing
CQ
Gui Yang College of Traditional Chinese Medicine, Guiyang 550002, China.
Lin
Ya-ping
YP
Jin
Feng-yun
FY
Zhang
Yong-ping
YP
D000818:Animals; D029748:Caryophyllaceae / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D005260:Female; D007249:Inflammation / Q000188:drug therapy; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy; D011897:Random Allocation
D004365:Drugs, Chinese Herbal