Expression of the inhibitor of DNA-binding (ID)-1 protein as an angiogenic mediator in tumour advancement of uterine cervical cancers.
The ID protein, an inhibitor of basic helix-loop-helix (HLH) transcription factors, has been involved in multiple cellular processes. To investigate the association between tumour advancement and ID expressions of uterine cervical cancers, the levels of ID-1, ID-2 and ID-3 mRNAs were determined by real-time reverse transcription-polymerase chain reaction and the histoscore with the localisation of ID-1 was determined by immunohistochemistry and patient survival in 60 patients. ID-1 histoscores and mRNA levels both significantly (P<0.05) increased in uterine cervical cancers according to clinical stage regardless of histopathological type or lymph node metastasis. Furthermore, the 36-month survival rate of the 30 patients with high ID-1 was poor (60%), whereas that of the other 30 patients with low ID-1 was significantly higher (83%). ID-1 histoscores and mRNA levels significantly (P<0.0001) correlated with microvessel counts in uterine cervical cancers. Tumour cells show mostly diffuse to strong cytoplasmic expression of ID-1 and also very faint expression in endothelial cells. Moreover, ID-1 expression not only correlated with microvessel counts but also correlated significantly with histoscore. Therefore, ID-1 might work on tumour advancement through angiogenic activity and is considered to be a candidate for a prognostic indicator in uterine cervical cancers.
Maw
M K
MK
Department of Obstetrics and Gynecology, Graduate School of Medicine, Gifu University School of Medicine, Gifu City, Japan.
Fujimoto
J
J
Tamaya
T
T
D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D000368:Aged; D043924:Angiogenesis Modulating Agents / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000096:biosynthesis*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger; D016019:Survival Analysis; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
D043924:Angiogenesis Modulating Agents; D014408:Biomarkers, Tumor; D051798:Inhibitor of Differentiation Protein 1; D012333:RNA, Messenger