title: Whole-cell recording using the perforated patch clamp technique.
abstract: Many ion channels, particularly potassium channels, are regulated by intracellular substances, such as nucleotides or Ca(2+). These modulators are washed out of the cell during standard whole-cell patch clamp recordings, or maintained at a particular concentration if they are included in the pipette solution. Perforated patch clamp recording permits electrical access between the cell and the patch pipette using pore-forming antibiotics such as nystatin or amphotericin B. These are permeable to small monovalent ions but present a physical barrier to the larger impermeable ions and molecules. This maintains the integrity of many cytoplasmic components including soluble second messengers, and also helps to prevent channel "run down".
authors.affiliation: Institute of Membrane and Systems Biology, University of Leeds, UK.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Lippiat
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D002460:Cell Line; D002468:Cell Physiological Phenomena; D004594:Electrophysiology / Q000379:methods; D006801:Humans; D007668:Kidney / Q000196:embryology; D008564:Membrane Potentials / Q000502:physiology; D009761:Nystatin / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*
chemical_list: D015221:Potassium Channels; D009761:Nystatin; D000666:Amphotericin B