title: Evaluation and optimization of hepatocyte culture media factors by design of experiments (DoE) methodology.
abstract: Optimization of cell culture media based on statistical experimental design methodology is a widely used approach for improving cultivation conditions. We applied this methodology to refine the composition of an established culture medium for growth of a human hepatoma cell line, C3A. A selection of growth factors and nutrient supplements were systematically screened according to standard design of experiments (DoE) procedures. The results of the screening indicated that the medium additives hepatocyte growth factor, oncostatin M, and fibroblast growth factor 4 significantly influenced the metabolic activities of the C3A cell line. Surface response methodology revealed that the optimum levels for these factors were 30 ng/ml for hepatocyte growth factor and 35 ng/ml for oncostatin M. Additional experiments on primary human hepatocyte cultures showed high variance in metabolic activities between cells from different individuals, making determination of optimal levels of factors more difficult. Still, it was possible to conclude that hepatocyte growth factor, epidermal growth factor, and oncostatin M had decisive effects on the metabolic functions of primary human hepatocytes.
authors.affiliation: Division of Biotechnology/IFM, Linköping University, 581 83, Linkoping, Sweden.
authors.forename: Jia
authors.initials: J
authors.lastname: Dong
authors.forename: Carl-Fredrik
authors.initials: CF
authors.lastname: Mandenius
authors.forename: Marc
authors.initials: M
authors.lastname: Lübberstedt
authors.forename: Thomas
authors.initials: T
authors.lastname: Urbaniak
authors.forename: Andreas K N
authors.initials: AK
authors.lastname: Nüssler
authors.forename: Daniel
authors.initials: D
authors.lastname: Knobeloch
authors.forename: Jörg C
authors.initials: JC
authors.lastname: Gerlach
authors.forename: Katrin
authors.initials: K
authors.lastname: Zeilinger