Delayed liver regeneration after partial hepatectomy in adiponectin knockout mice.
We previously demonstrated that adiponectin has anti-fibrogenic and anti-inflammatory effects in the liver of mouse models of various liver diseases. However, its role in liver regeneration remains unclear. The aim of this study was to determine the role of adiponectin in liver regeneration. We assessed liver regeneration after partial hepatectomy in wild-type (WT) and adiponectin knockout (KO) mice. We analyzed DNA replication and various signaling pathways involved in cell proliferation and metabolism. Adiponectin KO mice exhibited delayed DNA replication and increased lipid accumulation in the regenerating liver. The expression levels of peroxisome proliferator-activated receptor (PPAR) alpha and carnitine palmitoyltransferase-1 (CPT-1), a key enzyme in mitochondrial fatty acid oxidation, were decreased in adiponectin KO mice, suggesting possible contribution of altered fat metabolism to these phenomena. Collectively, the present results highlight a new role for adiponectin in the process of liver regeneration.
Ezaki
Hisao
H
Department of Gastroenterology and Hepatology, Osaka University, Graduate School of Medicine, 2-2, K1, Yamadaoka, Suita, Osaka 565-0871, Japan.
Yoshida
Yuichi
Y
Saji
Yukiko
Y
Takemura
Takayo
T
Fukushima
Juichi
J
Matsumoto
Hitoshi
H
Kamada
Yoshihiro
Y
Wada
Akira
A
Igura
Takumi
T
Kihara
Shinji
S
Funahashi
Tohru
T
Shimomura
Iichiro
I
Tamura
Shinji
S
Kiso
Shinichi
S
Hayashi
Norio
N
D052242:Adiponectin / Q000235:genetics / Q000502:physiology; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000096:biosynthesis; D002453:Cell Cycle; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D008115:Liver Regeneration / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D010084:Oxidation-Reduction; D047493:PPAR alpha / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D014280:Triglycerides / Q000378:metabolism
D052242:Adiponectin; C499919:Adipoq protein, mouse; D005227:Fatty Acids; D047493:PPAR alpha; D012333:RNA, Messenger; D014280:Triglycerides; D002334:Carnitine O-Palmitoyltransferase