STR profiles from DNA samples with "undetected" or low quantifiler results.
Screening methods capable of identifying DNA samples that will not yield short tandem repeat (STR) profiles are desired. In the past, quantitation methods have not been sensitive enough for this purpose. In this study, low level DNA samples were used to assess whether Quantifiler has a minimum quantitation value below which STR profiles would consistently fail to be detected. Buccal swabs were obtained and the DNA extracted, quantified, and serially diluted to concentrations ranging from 0.002 to 0.250 ng/microL. Samples were analyzed once with Quantifiler, followed by Profiler Plus amplification and capillary electrophoresis analysis. An absolute minimum value below which STR results were unobtainable could not be defined. From the 96 low level samples tested, STR loci (including one full profile) were successfully amplified and detected from 27% of the samples "undetected" by Quantifiler. However, no STR alleles were detected in 73% of these "undetected" samples, indicating that Quantifiler data may be useful for predicting STR typing success.
Cupples
Catherine M
CM
Laboratory Corporation of America, Forensic Identity Department, 1912 Alexander Drive, Research Triangle Park, NC 27709, USA.
Champagne
Jarrod R
JR
Lewis
Kristen E
KE
Cruz
Tracey Dawson
TD
D000328:Adult; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D019075:Electrophoresis, Capillary; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences; D055815:Young Adult
D004247:DNA