title: Neovascularization in an arterio-venous loop-containing tissue engineering chamber: role of NADPH oxidase.
abstract: Using an in vivo arterio-venous loop-containing tissue-engineering chamber, we have created a variety of vascularized tissue blocks, including functional myocardium. The viability of the transplanted cells is limited by the rate of neovascularization in the chamber. A Nox2-containing nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is thought to have a critical role in ischaemic angiogenesis. In this study we investigated whether NADPH oxidase is involved in the neovascularization process in the tissue-engineering chamber. New blood vessels originating from the venous and the arterial ends of the loop could be identified after 3 days, and the vessel density (by lectin staining) peaked after 7 days and was maintained for at least 14 days. This was accompanied by granulation tissue formation and concomitant increase in the mRNA level of Nox4 NADPH oxidase. Although the total level of Nox2 mRNA in the chamber tissue decreased from day 3 to day 7, immunohistochemistry identified a strong expression of Nox2 in the endothelial cells of the new vessels. In human microvascular endothelial cells, the NADPH oxidase inhibitor apocynin reduced NADPH oxidase activity and inhibited the angiogenic responses in vitro. Local treatment with the NADPH oxidase inhibitors apocynin or gp91ds-tat peptide significantly suppressed the vessel growth in the chamber. In conclusion, NADPH oxidase-dependent redox signalling is important for neovascularization in this novel tissue-engineering chamber in vivo, and boosting this signalling might be a new approach to extending vascularization and tissue growth.
authors.affiliation: Bernard O'Brien Institute of Microsurgery, the University of Melbourne, Victoria, Australia. fjiang@unimelb.edu.au
authors.forename: F
authors.initials: F
authors.lastname: Jiang
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.forename: I
authors.initials: I
authors.lastname: Hashimoto
authors.forename: B S
authors.initials: BS
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Bortolotto
authors.forename: W A
authors.initials: WA
authors.lastname: Morrison
authors.forename: G J
authors.initials: GJ
authors.lastname: Dusting
mesh_terms: D000818:Animals; D001163:Arteriovenous Anastomosis / Q000378:metabolism*; D001808:Blood Vessels / Q000473:pathology; D002254:Carbonates / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004867:Equipment Design; D005263:Femoral Artery / Q000378:metabolism; D005268:Femoral Vein / Q000378:metabolism; D017408:Guidelines as Topic; D007150:Immunohistochemistry; D008297:Male; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013481:Superoxides / Q000378:metabolism; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*
chemical_list: D002254:Carbonates; D011108:Polymers; D012333:RNA, Messenger; D013481:Superoxides; D019255:NADPH Oxidases