Adaptation of hybridomas to protein-free media results in a simplified two-step immunoglobulin M purification process.
An IgM antibody was purified from hybridoma supernatant containing serum using a three-step purification process comprising of tangential flow filtration, anion-exchange chromatography and size-exclusion chromatography. Recovery and purity were significantly improved upon adaptation of the hybridoma to serum-free media. The process could even be simplified by omitting the initial tangential flow filtration step. Even with a two-step purification process a purity of >98% and a recovery of >60% was obtained.
Lee
Jeremy
J
Bioprocessing Technology Institute, Agency for Science, Technology and Research, 20 Biopolis Way, No. 06-01 Centros, Singapore 138668, Singapore.
Tscheliessnig
Anne
A
Chen
Allen
A
Lee
Yih Yean
YY
Adduci
Gloria
G
Choo
Andre
A
Jungbauer
Alois
A
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D016895:Culture Media, Serum-Free; D006825:Hybridomas / Q000276:immunology*; D007075:Immunoglobulin M / Q000302:isolation & purification*; D051379:Mice
D003470:Culture Media; D016895:Culture Media, Serum-Free; D007075:Immunoglobulin M