Success of murine embryo transfer increased by a modified transfer pipette.
Embryos transfer is one of the most critical steps for generating genetically modified mice. Because of the limitations of the current transfer equipment and techniques, such as discharge of freshly transferred embryos and the necessity of sophisticated transfer skills, the success ratio of implantation may not well satisfy the requirements for mass production of high quality animals in the field of genetically modified mice. In this study, we describe a modified embryo transfer pipette with a syringe-like tip that can easily be applied to mouse embryo transfer. Using this improved method, we show that the procedures for mouse embryo implantation are simplified and easier to perform; moreover, the viability rate of mouse embryos is 20% higher than that achieved with conventional methods. Our modified tool and improved transfer technique are effective, time-saving and less invasive, resulting in increased success of embryo transfer.
Zhang
Zheng
Z
Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
Lv
Xiaoyan
X
Wang
Yidong
Y
Chen
Ye
Y
Zheng
Rong
R
Sun
Huan
H
Bian
Guohui
G
Xiao
Yan
Y
Li
Qingwei
Q
Yang
Qiutan
Q
Ai
Jianzhong
J
Duan
Jingjing
J
Tan
Ruizhi
R
Liu
Yuhang
Y
Yang
Yang
Y
Wei
Yuquan
Y
Zhou
Qin
Q
D000818:Animals; D004526:Efficiency; D004624:Embryo Transfer / Q000295:instrumentation* / Q000379:methods*; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D005818:Genetic Engineering / Q000295:instrumentation / Q000379:methods; D051379:Mice / Q000196:embryology*; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D008954:Models, Biological; D011247:Pregnancy