title: Measurement of benzophenones in human urine samples by stir bar sorptive extraction and thermal desorption-gas chromatography-mass spectrometry.
abstract: Determination of benzophenones (BPs) in human urine samples by stir bar sorptive extraction (SBSE) and thermal desorption (TD)-gas chromatography-mass spectrometry (GC-MS) is described. As analytes, BP, its metabolites benzhydrol (BP-OH) and 2-hydroxybenzophenone (2OH-BP), and its derivatives 2-hydroxy-4-methoxybenzophenone (BP-3) and 2-hydroxy-4-methoxy-4'-methylbenzophenone (BP-10) were selected. After enzymatic hydrolysis, a polydimethylsiloxane (PDMS) stir bar was placed in a urine sample diluted 1:1 with water and stirred for 60 min at room temperature. The limit of quantification (LOQ) of BPs is 0.2-0.5 ng ml(-1) (ppb). The method showed linearity over the calibration range (0.2-10 or 0.5-10 ng ml(-1)), and the correlation coefficients were equal to or higher than 0.993 for all of the analytes. The average recoveries of BPs were equal to or higher than 98.7% (RSD: 1.5-4.8%, n = 6).
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa, Tokyo 142-8501, Japan.
authors.forename: Migaku
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Rie
authors.initials: R
authors.lastname: Ito
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Honda
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Endo
authors.forename: Noriya
authors.initials: N
authors.lastname: Okanouchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Saito
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Seto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
mesh_terms: D001577:Benzophenones / Q000378:metabolism / Q000652:urine*; D002138:Calibration; D004798:Enzymes / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000592:standards; D006801:Humans; D006868:Hydrolysis
chemical_list: D001577:Benzophenones; D004798:Enzymes