Telomerase inhibiting activity in vitro from natural resources, marine algae extracts.
We attempted to screen for telomerase inhibitingactivity in vitro from a total of 304 marine algae samples which were collected from various Japancoasts by nonradioisotope telomeric repeatamplification protocol (Non-RI TRAP) assay using humanleukemia MOLT-4 cells. Ten of the MeOH extracts andtwo of the PBS extracts from those algal samplesshowed telomerase inhibiting activity. In particular,the MeOH extract from a green alga, Caulerpasertularioides strongly inhibited telomerase activitywhen added to MOLT-4 cell culture at a level of 1.25%(v/v), suggesting that it may be possible to developa novel anti-cancer agent in view of its specificanti-telomeric property.
Kanegawa
K
K
Marine and Highland Bioscience Center, Saga University, 152-1 Shonan-cho, Karatsu, Saga, 847-0021, Japan.
Harada
H
H
Myouga
H
H
Katakura
Y
Y
Shirahata
S
S
Kamei
Y
Y