Proteomics analysis of A375 human malignant melanoma cells in response to arbutin treatment.
Although the toxicogenomics of A375 human malignant melanoma cells treated with arbutin have been elucidated using DNA microarray, the proteomics of the cellular response to this compound are still poorly understood. In this study, we performed proteomic analyses to investigate the anticancer effect of arbutin on the protein expression profile in A375 cells. After treatment with arbutin (8 microg/ml) for 24, 48 and 72 h, the proteomic profiles of control and arbutin-treated A375 cells were compared, and 26 differentially expressed proteins (7 upregulated and 19 downregulated proteins) were identified by MALDI-Q-TOF MS and MS/MS. Among these proteins, 13 isoforms of six identical proteins were observed. Bioinformatic tools were used to search for protein function and to predict protein interactions. The interaction network of 14 differentially expressed proteins was found to be correlated with the downstream regulation of p53 tumor suppressor and cell apoptosis. In addition, three upregulated proteins (14-3-3G, VDAC-1 and p53) and five downregulated proteins (ENPL, ENOA, IMDH2, PRDX1 and VIME) in arbutin-treated A375 cells were validated by RT-PCR analysis. These proteins were found to play important roles in the suppression of cancer development.
Nawarak
Jiraporn
J
Institute of Biological Chemistry and Genomics Research Center, Academia Sinica, Taipei 11529, Taiwan.
Huang-Liu
Rosa
R
Kao
Shao-Hsuan
SH
Liao
Hsien-Hua
HH
Sinchaikul
Supachok
S
Chen
Shui-Tein
ST
Cheng
Sun-Long
SL
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001104:Arbutin / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D008545:Melanoma / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D011485:Protein Binding; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation
D000970:Antineoplastic Agents; D001104:Arbutin