title: A strategy for screening an inhibitor of viral silencing suppressors, which attenuates symptom development of plant viruses.
abstract: To find out whether we can control plant virus diseases by blocking viral RNA silencing suppressors (RSSs), we developed a strategy to screen inhibitors that block the association of RSSs with siRNAs using a surface plasmon resonance assay. The screened chemicals were tested in competition with RSSs for binding to siRNAs using a mobility shift assay. We then confirmed that tested chemicals actually inhibited the RSS activity in vivo using a protoplast assay which was developed for this purpose. This entire system can be adapted to screening inhibitors of not only plant viruses but also some animal viruses possessing RSSs.
authors.affiliation: Laboratory of Cell Biology and Manipulation, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Hanako
authors.initials: H
authors.lastname: Shimura
authors.forename: Takako
authors.initials: T
authors.lastname: Fukagawa
authors.forename: Ayano
authors.initials: A
authors.lastname: Meguro
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yamada
authors.forename: Mahito
authors.initials: M
authors.lastname: Oh-Hira
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Sano
authors.forename: Chikara
authors.initials: C
authors.lastname: Masuta
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D055808:Drug Discovery; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000187:drug effects* / Q000235:genetics; D034622:RNA Interference / Q000187:drug effects*; D034741:RNA, Small Interfering / Q000187:drug effects; D012367:RNA, Viral / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D034741:RNA, Small Interfering; D012367:RNA, Viral