[Ornithine decarboxylase and S-adenosylmethionine decarboxylase bi-antisense virus inhibit growth and invasion of esophageal cancer cell line Eca109].
OBJECTIVE
Esophageal carcinoma is one of the most common malignant tumors in China. It is reported that the content and biosynthesis of polyamine in esophageal cancer is markedly increased. This study was to investigate inhibitory effects of both antisense ornithine decarboxylase (ODCas) and S-adenosylmethionine bi-antisense (AdoMetDCas) on polyamine biosynthesis, cell proliferation and invasion of esophageal cancer cell line Eca109.


METHODS
An adenoviral vector Ad-ODC-AdoMetDCas containing antisense sequences of ODCse and AdoMetDCas was used. Cell proliferation was observed by counting viable cells or BrdU labeling. Protein expressions of ODC and AdoMetDC and the polyamine content in Eca109 cells were measured by Western blot and high performance liquid chromatography (HPLC), respectively. Invasion of Eca109 cells in vitro was detected using Matrigel invasion assay. Furthermore, the anti-proliferation effect of Ad-ODC-AdoMetDCas on Eca109 cell xenografts in nude mice was evaluated.


RESULTS
Ad-ODC-AdoMetDCas significantly inhibited proliferation of Eca109 cells(P<0.05) and protein expressions of ODC and AdoMetDC. Transfection of Ad-ODC-AdoMetDCas significantly decreased synthesis of three polyamines in Eca109 cells, which were putrescine (Put), spermidine (Spd) and spermine (Spm) (P<0.05). Ad-ODC-AdoMetDCas dramatically suppressed invasiveness of Eca109 cells in vitro(P<0.05). In addition, compared with Ad-GFP, Ad-ODC-AdoMetDCas significantly suppressed the growth of Eca109 cell xenografts in nude mice.


CONCLUSIONS
Ad-ODC-AdoMetDCas significantly inhibits cell proliferation and invasion in esophageal cancer Eca109 cells.
Song
Xu
X
Department of Thoracic Surgery, Qi Lu Hospital, Shandong University, Shandong, Jinan, China.
Tian
Hui
H
Liu
Xian-Xi
XX
Zhang
Bing
B
Li
Wen-Jun
WJ
Xu
Jie
J
D012437:Adenosylmethionine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism; D016372:RNA, Antisense; D011897:Random Allocation; D014162:Transfection
D011073:Polyamines; D016372:RNA, Antisense; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase