Receptor for activated C-kinase 1, a novel binding partner of adiponectin receptor 1.
Adiponectin is an adipose tissue derived hormone with anti-diabetic and insulin-sensitizing properties. Two adiponectin receptors, AdipoR1 and AdipoR2, have recently been identified, yet the signaling pathways triggered through adiponectin receptors remain to be elucidated. Using a yeast two-hybrid screen, we identified an adaptor protein, receptor for activated protein kinase C1 (RACK1), as an interacting partner of human AdipoR1. RACK1 was confirmed to interact with AdipoR1 by co-immunoprecipitation and co-localization analysis in mammalian cells. The interaction was enhanced by adiponectin stimulation. In addition, the knockdown of RACK1 by RNA interference inhibited adiponectin-stimulated glucose uptake in HepG2 cells. These results suggest that RACK1 may act as a key bridging factor in adiponectin signaling transduction through interacting with AdipoR1.
Xu
Yizhou
Y
Department of Cardiology, Hangzhou the First People's Hospital, 261 Wansha Road, Hangzhou 310006, China.
Wang
Ningfu
N
Ling
Feng
F
Li
Peizhang
P
Gao
Yan
Y
D052242:Adiponectin / Q000494:pharmacology; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D000075370:Receptors for Activated C Kinase; D054419:Receptors, Adiponectin / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
C475418:ADIPOR1 protein, human; D052242:Adiponectin; D009363:Neoplasm Proteins; C419368:RACK1 protein, human; D000075370:Receptors for Activated C Kinase; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins; D005947:Glucose