title: Integrated fuzzy concentration addition-independent action (IFCA-IA) model outperforms two-stage prediction (TSP) for predicting mixture toxicity.
abstract: Mixture toxicities were determined for 12 industrial organic chemicals bearing four different modes of toxic action (MOAs) to Vibrio fischeri, to compare the predictability of the integrated fuzzy concentration addition-independent action (IFCA-IA) model and the two-stage prediction (TSP) model. Three mixtures were designed: The first and second mixtures were based on the ratios of each component at the 1% and 50% effect concentrations (EC(1) and EC(50)), respectively; and the third mixture contained an equimolar ratio of individual components. For the EC(1), EC(50) and equimolar ratio, prediction errors from the IFCA-IA model at the 50% experimental mixture effects were 0.3%, 6% and 0.6%, respectively; while for the TSP model, the corresponding errors were 2.8%, 19% and 24%, respectively. Thus, the IFCA-IA model performed better than the TSP model. The IFCA-IA model calculated two weight coefficients from the molecular structural descriptors, which weigh the relation between concentration addition (CA) and independent action (IA) through the fuzzy membership functions. Thus, MOAs are not pre-requisites for mixture toxicity prediction by the IFCA-IA approach, implying the practicability of this method in toxicity assessment of mixtures.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), Department of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Wang
authors.forename: Jingwen
authors.initials: J
authors.lastname: Chen
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiyun
authors.initials: X
authors.lastname: Cai
authors.forename: Xianliang
authors.initials: X
authors.lastname: Qiao
authors.forename: Yuying
authors.initials: Y
authors.lastname: Dong
mesh_terms: D048908:Aliivibrio fischeri / Q000187:drug effects*; D017143:Fuzzy Logic; D007220:Industrial Waste; D009930:Organic Chemicals / Q000633:toxicity*
chemical_list: D007220:Industrial Waste; D009930:Organic Chemicals