The antigen-specific memory CD8+ T-cell response induced by BCG in cattle resides in the CD8+gamma/deltaTCR-CD45RO+ T-cell population.
Tuberculosis (TB) remains a worldwide leading cause of death among infectious diseases. Development of safer and more efficacious vaccines requires a basic understanding of the protective mechanisms induced by BCG. Here we show that vaccination of cattle with BCG induces CD8+gamma/deltaTCR-CD45RO+ T-cells that can produce IFN-gamma, up-regulate transcription and expression of perforin, lyse BCG-infected monocyte-derived macrophages (MoMvarphi) and contribute to a reduction in the number of intracellular mycobacteria. We also observed BCG-induced CD8+ responses in vivo. After infection of cattle with Mycobacterium bovis, CD8+gamma/deltaTCR-CD45RO+ cells responded more strongly to M. bovis-infected MoMvarphi than to BCG-infected MoMvarphi. These results indicate that the antigen-specific CD8+ memory response resides in the CD8+gamma/deltaTCR-CD45RO+ cell population.
Hogg
Alison E
AE
Institute for Animal Health, Compton, Nr. Newbury, Berkshire RG20 7NN, UK.
Worth
Andrew
A
Beverley
Peter
P
Howard
Christopher J
CJ
Villarreal-Ramos
Bernardo
B
D000818:Animals; D000941:Antigens / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002417:Cattle; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008213:Lymphocyte Activation; D009163:Mycobacterium bovis / Q000276:immunology; D054353:Perforin / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D014380:Tuberculosis, Bovine / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*
D000941:Antigens; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D016692:Receptors, Antigen, T-Cell, gamma-delta; D054353:Perforin; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens