EdU, a new thymidine analogue for labelling proliferating cells in the nervous system.
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a covalent bond via the "click" chemistry reaction (the Huisgen 1,3-dipolar cycloaddition reaction of an organic azide to a terminal acetylene). Unlike the commonly used BrdU, EdU detection requires no heat or acid treatment. It is quick and easy and compatible with multiple probes for fluorescence immunochemistry, facilitating the characterisation of proliferating cells at high resolution.
Chehrehasa
Fatemah
F
National Centre for Adult Stem Cell Research, Eskitis Institute for Cellular & Molecular Therapies, Griffith University, Brisbane, Qld 4111, Australia. f.chehrehasa@griffith.edu.au
Meedeniya
Adrian C B
AC
Dwyer
Patrick
P
Abrahamsen
Greger
G
Mackay-Sim
Alan
A
D000199:Actins / Q000378:metabolism; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002461:Cell Line, Transformed; D004305:Dose-Response Relationship, Drug; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007211:Indoles; D051379:Mice; D008810:Mice, Inbred C57BL; D009420:Nervous System / Q000166:cytology* / Q000187:drug effects; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010671:Phenylurea Compounds / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D011247:Pregnancy; D013936:Thymidine / Q000378:metabolism
D000199:Actins; D005904:Glial Fibrillary Acidic Protein; D007211:Indoles; D010671:Phenylurea Compounds; C007293:DAPI; C032766:ethylene diurea; D001973:Bromodeoxyuridine; D013936:Thymidine