Improvement of L-lactate production by CYB2 gene disruption in a recombinant Saccharomyces cerevisiae strain under low pH condition.
Using a DNA microarray, we found that expression of the genes related to lactate metabolism was upregulated in a lactate-producing recombinant Saccharomyces cerevisiae strain. Disruption of the CYB2 gene encoding L-lactate dehydrogenase improved the L-lactate production by S. cerevisiae under low pH condition.
Ookubo
Aki
A
Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Japan.
Hirasawa
Takashi
T
Yoshikawa
Katsunori
K
Nagahisa
Keisuke
K
Furusawa
Chikara
C
Shimizu
Hiroshi
H
D005818:Genetic Engineering; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase