title: KIF5C: a new binding partner for protein kinase CK2 with a preference for the CK2alpha' subunit.
abstract: Protein kinase CK2 is a highly conserved serine/threonine kinase that is ubiquitously expressed in eukaryotic cells. CK2 is a constitutively active tetrameric enzyme composed of two catalytic alpha and/or alpha'-subunits and two regulatory beta-subunits. There is increasing evidence that the individual subunits may have independent functions and that they are asymmetrically distributed inside the cell. To gain a better understanding of the functions of the individual subunits, we employed a yeast-two-hybrid screen with CK2alpha and CK2alpha'. We identified the motor neuron protein KIF5C as a new binding partner for CK2. The interaction found in the yeast-two-hybrid screen was confirmed by co-sedimentation analysis on a sucrose density gradient and by co-immunoprecipitation analysis. Pull-down experiments and surface plasmon resonance spectrometry revealed a direct binding of KIF5C to CK2alpha'. Co-localization studies with neuroblastoma cells, bone marrow and with primary neurons confirmed the biochemical analysis that KIF5C preferentially bound to CK2alpha'.
authors.affiliation: Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Gebäude 44, 66424, Homburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Schäfer
authors.forename: C
authors.initials: C
authors.lastname: Götz
authors.forename: J
authors.initials: J
authors.lastname: Dudek
authors.forename: A
authors.initials: A
authors.lastname: Hessenauer
authors.forename: U
authors.initials: U
authors.lastname: Matti
authors.forename: M
authors.initials: M
authors.lastname: Montenarh
mesh_terms: D000818:Animals; D047390:Casein Kinase II / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D020798:Two-Hybrid System Techniques
chemical_list: D020033:Protein Isoforms; D021122:Protein Subunits; D047390:Casein Kinase II; C514435:KIF5C protein, human; D016547:Kinesins