title: Transdentinal diffusion and cytotoxicity of self-etching adhesive systems.
abstract: OBJECTIVE
  To evaluated the transdentinal diffusion and subsequent cytotoxicity of self-etching adhesives on odontoblast-like cells.
  
  
  METHODS
  Sixty dentin disks (0.4-mm thick) were produced from human molars and divided into six groups (n = 10). The dentin disks were placed in in vitro pulp chambers where MDPC-23 cells were planted on 0.28 cm(2) of exposed dentin on the pulpal side. The adhesives Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Adper Prompt (PR), and Xeno III (XE) were applied on the occlusal side. Single Bond (SB) was used as positive and phosphate buffer solution (PBS) as negative control. The cytotoxicity was measured by MTT assay and cell characteristics were assessed by SEM. The transdentinal diffusion was qualified by GC/MS.
  
  
  RESULTS
  Kruskal-Wallis and Mann-Whitney tests demonstrated a significant difference among the adhesives and PBS. Cellular viability reduction promoted by the self-etching systems was lower than that of SB (53.1%), except for CSE. Cell metabolism was reduced in 47.8%, 42.1%, 28.0%, and 46.5% for CSE, CPB, PR, and XE, respectively. HEMA was identified as the main diffused component.
  
  
  CONCLUSIONS
  Components from all investigated self-etching adhesive systems were able to diffuse through the dentin resulting in significant reduction of the cellular metabolism.
authors.affiliation: Department of Oral Clinic, Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Department of Pediatric Dentistry, School of Dentistry, PUC-Minas, Belo Horizonte, MG, Brazil.
authors.forename: Célia Regina Moreira
authors.initials: CR
authors.lastname: Lanza
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: de Souza Costa
authors.forename: Maysa
authors.initials: M
authors.lastname: Furlan
authors.forename: Alberto
authors.initials: A
authors.lastname: Alécio
authors.forename: Josimeri
authors.initials: J
authors.lastname: Hebling
mesh_terms: D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003804:Dentin / Q000187:drug effects; D003806:Dentin Permeability / Q000187:drug effects*; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D008963:Molar; D009804:Odontoblasts / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D019279:Resin Cements / Q000494:pharmacology; D014014:Tissue Adhesives / Q000009:adverse effects* / Q000032:analysis
chemical_list: C438313:Clearfil SE Bond; D019279:Resin Cements; D014014:Tissue Adhesives; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate