Separation and quantitative analysis of alkyl sulfate ethoxymers by HPLC.
Separation of alkyl sulfate ethoxymers is investigated on various high-performance liquid chromatography (HPLC) stationary phases: Acclaim C18 Surfactant, Surfactant C8, and Hypercarb. For a fixed alkyl chain length, ethoxymers are eluted in the order of increasing number of ethoxylated units on Acclaim C18 Surfactant, whereas a reversed elution order is observed on Surfactant C8 and Hypercarb. Moreover, on an Acclaim C18 Surfactant column, non-ethoxylated compounds are eluted in their ethoxymers distribution and the use of sodium acetate additive in mobile phase leads to a co-elution of ethoxymers. HPLC stationary phases dedicated to surfactants analysis are evaluated by means of the Tanaka test. Surfactant C8 presents a great silanol activity whereas Acclaim C18 Surfactant shows a high steric selectivity. For alkyl sulfates, linearity of the calibration curve and limits of detection and quantitation are evaluated. The amount of sodium laureth sulfate raw material found in commercial body product is in agreement with the specification of the manufacturer.
Morvan
Julien
J
Laboratoire Synthèse et Chromatographie, Sciences et Méthodes Séparatives, UPRES EA 3233, Université de Rouen, rue Tesnières, F-76821 Mont Saint Aignan Cedex, France.
Hubert-Roux
Marie
M
Agasse
Valérie
V
Cardinael
Pascal
P
Barbot
Florence
F
Decock
Gautier
G
Bouillon
Jean-Philippe
JP
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D013463:Sulfuric Acid Esters / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*
D013463:Sulfuric Acid Esters