title: Border disease virus (BDV) infections of small ruminants in Turkey: a new BDV subgroup?
abstract: Blood samples from sheep and/or goats from eight small ruminant flocks in the Turkish provinces of Aydin and Burdur were tested for the presence of Pestiviruses using an antigen-capture ELISA. From clinically affected animals, pathological and immunohistochemical findings were recorded. Post mortem examination of a virus-positive lamb showing abnormal fleece and paralysis of the hind legs revealed nonsuppurative meningoencephalomyelitis with hypomyelinogenesis. By immunohistochemistry Pestivirus antigen was detected in all parts of the brain including cerebellum, cerebral hemispheres and midbrain. Two Pestivirus isolates from a sheep and a goat kid, respectively, were isolated from samples that were positive in the antigen-capture ELISA. Genetic typing using the 5'-NTR (288bp) and N(pro) (738bp) showed that both were Border disease virus (BDV) isolates. By phylogenetic analysis, they formed a cluster clearly separated from the known clusters BDV-1 to BDV-6 and might therefore represent a new subgroup (BDV-7?). This is the first report confirming the occurrence and partial characterisation of BDV infection in small ruminants in Turkey.
authors.affiliation: Department of Virology, Ankara University, Faculty of Veterinary Medicine, 06110 Dişkapi-Ankara, Turkey. oguzoglu@veterinary.ankara.edu.tr
authors.forename: T C
authors.initials: TC
authors.lastname: Oguzoglu
authors.forename: M T
authors.initials: MT
authors.lastname: Tan
authors.forename: N
authors.initials: N
authors.lastname: Toplu
authors.forename: A B
authors.initials: AB
authors.lastname: Demir
authors.forename: S
authors.initials: S
authors.lastname: Bilge-Dagalp
authors.forename: T
authors.initials: T
authors.lastname: Karaoglu
authors.forename: A
authors.initials: A
authors.lastname: Ozkul
authors.forename: F
authors.initials: F
authors.lastname: Alkan
authors.forename: I
authors.initials: I
authors.lastname: Burgu
authors.forename: L
authors.initials: L
authors.lastname: Haas
authors.forename: I
authors.initials: I
authors.lastname: Greiser-Wilke
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis / Q000097:blood; D001882:Border Disease / Q000453:epidemiology*; D018083:Border disease virus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D054022:Cerebrum / Q000821:virology; D005838:Genotype; D005843:Geography; D015511:Goat Diseases / Q000097:blood / Q000453:epidemiology / Q000821:virology; D006041:Goats / Q000821:virology; D006614:Hindlimb / Q000821:virology; D010576:Pestivirus / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000453:epidemiology / Q000821:virology; D014421:Turkey / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral