The application of gradient reversed-phase high-performance liquid chromatography to the pK(a) and log k(w) determination of polyprotic analytes.
The purpose of this work was to propose a theoretical model of the combined pH/organic modifier gradient in reversed-phase high-performance liquid chromatography (RP HPLC) with special emphasis on its applicability to polyprotic analytes. The model was developed and approximated to be useful for a data set comprising organic modifier gradients obtained at different pH changes and different gradient durations. It was evaluated regarding its ability to describe experimental data. The chromatographic pK(a) and lipophilicity parameter, log k(w), were obtained by fitting to the proposed model and comparing to the literature values.
Wiczling
Paweł
P
Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Gen. J. Hallera 107, 80-416 Gdańsk, Poland. wiczling@amg.gda.pl
Waszczuk-Jankowska
Małgorzata
M
Markuszewski
Michał Jan
MJ
Kaliszan
Roman
R
D000465:Algorithms; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008962:Models, Theoretical; D018892:Proton-Motive Force; D012997:Solvents / Q000737:chemistry
D012997:Solvents