title: Identification of crude drugs from Chinese medicinal plants of the genus Bupleurum using ribosomal DNA ITS sequences.
abstract: To determine the variation of rDNA ITS sequences in medicinal plants of the genus Bupleurum and identify corresponding DNA molecular markers associated with medicinally important members, ITS regions were amplified, sequenced, and analyzed by DNAssist version 2.2. We found that the homologous alignment of ITS sequences between members of the genus Bupleurum and associated out-groups was lower than 75%, while within-group alignment was greater than 87%. The conclusion can be drawn that ITS sequences can be used as reliable molecular markers for the identification of Radix Bupleuri.
authors.affiliation: School of Pharmacy, Fudan University, Shanghai, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Ke-ke
authors.initials: KK
authors.lastname: Huo
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chao
authors.forename: Sheng-li
authors.initials: SL
authors.lastname: Pan
mesh_terms: D029972:Bupleurum / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D005819:Genetic Markers; D010946:Plants, Medicinal / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers