title: Microsupport with two-dimensional geometry (2D-MS) 4. Temperature-induced detachment of anchorage-dependent CHO-K1 cells from cryoresponsive MicroHex((R)) (CryoHex).
abstract: Tissue culture flasks were activated by electron beam irradiation and subsequently treated with N-isopropylacrylamide to make them cryoresponsive. Leaving such 'cryoflasks' unattended for 10 minutes at room temperature sufficed to almost completely detach the anchorage-dependent cells. MicroHex((R)), a polystyrene-based tissue culture microsupport with two-dimensional geometry, was handled in the same way to obtain CryoHex, i.e. a cryoresponsive MicroHex from which anchorage-dependent cells could be detached by exposure to low temperature (4-20 degrees C). Experimental conditions were determined allowing one to detach the cells from small and large microsupport culture volumes. Cells detached from CryoHex by exposure to low temperature displayed a high cell viability and, upon subcultivation on MicroHex((R)), did not show any alteration of their growth kinetics.
authors.affiliation: 4C Biotech s.a., Rue de la Marlette 14, Seneffe, B-7180, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Kenda-Ropson
authors.forename: S
authors.initials: S
authors.lastname: Lenglois
authors.forename: A O A
authors.initials: AO
authors.lastname: Miller