Genetic analysis of a bovine viral diarrhea virus 2 isolate from Slovakia.
The identification and genetic characterization of bovine viral diarrhea virus (BVDV) isolate 17237 detected in western Slovakia is described. The analysis of 5'-untranslated region (5'-UTR), autoprotease (Npro) gene, and structural genes (C, Erns, E1, E2) was carried out. The percentage of nucleotide and deduced amino acid identity in analyzed genes implied that the isolate was closely related to the bovine viral diarrhea virus 2 (BVDV-2). Furthermore, the phylogenetic analysis revealed that this isolate fall into BVDV-2b subtype that is sporadic in Europe. The cleavage sites between viral proteins were similar to the ones of a reference strain of BVDV-2.
Novácková
M
M
University of Veterinary Medicine, Kosice, Slovak Republic.
Jacková
A
A
Kolesárová
M
M
Vilcek
S
S
D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030243:Hemorrhagic Syndrome, Bovine / Q000453:epidemiology* / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D018154:Slovakia / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
D020121:5' Untranslated Regions; C507353:Npro protein, bovine viral diarrhea virus; D014764:Viral Proteins; D015678:Viral Structural Proteins