title: In situ imaging reveals different responses by naïve and memory CD8 T cells to late antigen presentation by lymph node DC after influenza virus infection.
abstract: Pulmonary influenza infection causes prolonged lymph node hypertrophy while processed viral antigens continue to be presented to virus-specific CD8 T cells. We show that naïve, but not central/memory, nucleoprotein (NP)-specific CD8 T cells recognized antigen-bearing CD11b(+) DC in the draining lymph nodes more than 30 days after infection. After these late transfers, the naïve CD8 T cells underwent an abortive proliferative response in the mediastinal lymph node (MLN), where large clusters of partially activated cells remained in the paracortex until at least a week after transfer. A majority of the endogenous NP-specific CD8 T cells that were in the MLN between 30 and 50 days after infection also showed signs of a continuing response to antigen stimulation. A high frequency of endogenous NP-specific CD8 T cells in the MLN indicates that late antigen presentation may help shape the epitope dominance hierarchy during reinfection.
authors.affiliation: Department of Immunology, University of Connecticut, Farmington, CT 06032-1319, USA.
authors.forename: Kamal M
authors.initials: KM
authors.lastname: Khanna
authors.forename: Carolina C
authors.initials: CC
authors.lastname: Aguila
authors.forename: Jason M
authors.initials: JM
authors.lastname: Redman
authors.forename: Jenny E
authors.initials: JE
authors.lastname: Suarez-Ramirez
authors.forename: Leo
authors.initials: L
authors.lastname: Lefrançois
authors.forename: Linda S
authors.initials: LS
authors.lastname: Cauley
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology; D039481:CD11b Antigen / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D003713:Dendritic Cells / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009698:Nucleoproteins / Q000276:immunology; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D010641:Phenotype; D013997:Time Factors
chemical_list: D039481:CD11b Antigen; D009698:Nucleoproteins