title: Evaluation of trypsin treatment on the inactivation of bovine herpesvirus type 1 on in vitro produced pre-implantation embryos.
abstract: The aim of this study was to evaluate the efficiency of trypsin treatment on the inactivation of bovine herpesvirus type 1 (BoHV-1) on in vitro produced by fertilization and artificially infected bovine embryos. Bovine embryos on day 7 were exposed with 10 microl of BoHV-1, Los Angeles strain 10(7.5) TCID. These embryos and control embryos were divided in two groups: submitted to the sequential washes or to the trypsin treatment according to the International Embryo Transfer Society (IETS) guidelines. The embryos and the last washing drop of each group were used as inoculum to infect Madin Darby bovine kidney (MDBK) cells and submitted to nested PCR reaction using the primer that encodes the gene conserved region of virus glycoprotein gB. The data have shown that the control embryos and their last washing drop were negative. The exposed embryos that were treated with trypsin have shown positive results on the n-PCR and MDBK culture, and their last washing drop were negative. Our data have demonstrated that the trypsin treatment was not able to eliminate the BHV-1 of the embryos, suggesting an interaction between virus and embryo.
authors.affiliation: Animal Health Research Center, São Paulo Biological Institute, SP, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: D'Angelo
authors.forename: J A
authors.initials: JA
authors.lastname: Visintin
authors.forename: L J
authors.initials: LJ
authors.lastname: Richtzenhain
authors.forename: R F
authors.initials: RF
authors.lastname: Gonçalves
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001755:Blastocyst / Q000821:virology*; D002417:Cattle / Q000196:embryology* / Q000821:virology; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D005307:Fertilization in Vitro / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D007668:Kidney; D016133:Polymerase Chain Reaction; D014357:Trypsin / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D014357:Trypsin