title: Immersion oil for high-resolution live-cell imaging at 37 degrees C: optical and physical characteristics.
abstract: The use of normal immersion oil, developed for 23 degrees C, at 37 degrees C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37 degrees C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23 degrees C counterparts.
authors.affiliation: Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. l.oomen@nki.nl
authors.forename: L C J M
authors.initials: LC
authors.lastname: Oomen
authors.forename: R
authors.initials: R
authors.lastname: Sacher
authors.forename: H H J
authors.initials: HH
authors.lastname: Brocks
authors.forename: J M
authors.initials: JM
authors.lastname: Zwier
authors.forename: G J
authors.initials: GJ
authors.lastname: Brakenhoff
authors.forename: K
authors.initials: K
authors.lastname: Jalink
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D007668:Kidney / Q000166:cytology; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009821:Oils; D013696:Temperature
chemical_list: D009821:Oils