title: Prevalence and dissemination of the Ser315Thr substitution within the KatG enzyme in isoniazid-resistant strains of Mycobacterium tuberculosis isolated in Uruguay.
abstract: The aim of this study was to determine the prevalence of Ser315Thr substitution in isoniazid (INH)-resistant strains of Mycobacterium tuberculosis in Uruguay. The katG gene of 62 INH-resistant strains was analysed by an RFLP-PCR assay. PCR products were digested with MspI to detect Ser315Thr and Arg463Leu substitutions. A total of 16 of the 62 (26 %) INH-resistant strains analysed had a Ser315Thr substitution. Only one INH-resistant strain had an Arg463Leu substitution and two strains had a deletion in katG. Of the 16 strains with Ser315Thr, 15 showed different profiles using a double-repetitive-element PCR assay, demonstrating that there was no local dissemination of any particular strain. These findings are in agreement with published data from regions where the prevalence of tuberculosis (TB) is intermediate and may be due in part to the success of the local TB control programme.
authors.affiliation: Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Varela
authors.affiliation: Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
authors.forename: Sabina
authors.initials: S
authors.lastname: González
authors.affiliation: Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
authors.forename: Pilar
authors.initials: P
authors.lastname: Gadea
authors.affiliation: Laboratory Department, Honorary Anti-Tuberculosis Commission, 18 de Julio 2175, Montevideo, Uruguay.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Coitinho
authors.affiliation: Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
authors.forename: Inés
authors.initials: I
authors.lastname: Mota
authors.affiliation: Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
authors.forename: Gladys
authors.initials: G
authors.lastname: González
authors.affiliation: Department of Neurology, New York University School of Medicine, New York, USA.|Department of Immunology, School of Chemistry, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
authors.forename: Fernando
authors.initials: F
authors.lastname: Goñi
authors.affiliation: Laboratory Department, Honorary Anti-Tuberculosis Commission, 18 de Julio 2175, Montevideo, Uruguay.
authors.forename: Carlos
authors.initials: C
authors.lastname: Rivas
authors.affiliation: Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
authors.forename: Felipe
authors.initials: F
authors.lastname: Schelotto
mesh_terms: D019943:Amino Acid Substitution; D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002374:Catalase / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000382:microbiology; D014583:Uruguay / Q000453:epidemiology
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; D007538:Isoniazid