Cymantrene conjugation modulates the intracellular distribution and induces high cytotoxicity of a cell-penetrating peptide.
The conjugation of cymantrene CpMn(CO)(3) to cell-penetrating peptide hCT(18-32)-k7 alters the intracellular distribution in MCF-7 cells compared to the unmodified peptide, as visualized by fluorescence microscopy, and leads to an increased nuclear accumulation; the peptide and cymantrene compound themselves are not toxic, but the bioconjugate shows a significant cytotoxicity with an IC(50) value of 36 micromol l(-1).
Neundorf
Ines
I
Institut für Biochemie, Universität Leipzig, Brüderstr. 34, D-04103, Leipzig, Germany. ines.neundorf@uni-leipzig.de
Hoyer
Jan
J
Splith
Katrin
K
Rennert
Robert
R
Peindy N'dongo
Harmel W
HW
Schatzschneider
Ulrich
U
D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity*
D009942:Organometallic Compounds; D010455:Peptides; C044679:manganese cyclopentadienyl tricarbonyl