title: High density and scale-up cultivation of recombinant CHO cell line and hybridomas with porous microcarrier Cytopore.
abstract: Using porous microcarrier Cytopore and a low-serum medium supplement BIGBEF-3, we have successfully cultivated recombinant CHO cell line CL-11G producing prourokinase and hybridomas producing anti-prourokinase monoclonal antibody in Celligen 1.5 or 5 L bioreactor. The cell density obtained ranged from 1 to 2 x 107 cells mL-1. The yields of prourokinase and monoclonal antibody increased with increasing cell density. As the cells could spontaneously release from and reattach to porous microcarriers, it was very easy to scale-up the cultivation. Thus the bead to bead cell transfer method has been used to scale up the cultivation of CL-11G cells to a 20 L reactor-scale for the pilot production of prourokinase, and also to scale-up the culture of hybridomas for the production of monoclonal antibody for the purification of prourokinase.
authors.affiliation: Department of Cell Engineering, Institute of Biotechnology, 20 Dongdajie, Fengtai, Beijing, 100071, P.R. China.
authors.forename: C
authors.initials: C
authors.lastname: Xiao
authors.forename: Z
authors.initials: Z
authors.lastname: Huang
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Hu
authors.forename: W
authors.initials: W
authors.lastname: Qu
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.forename: G
authors.initials: G
authors.lastname: Liu