Sol-gel-based poliovirus-1 detector.
Hybrid sol-gel films were used to grow Buffalo Green Monkey kidney cell tissues, which were used for poliovirus-1 detection. It is shown that the sol-gel approach allows cutting the standard EPA procedure from 48 to 24h of detection time; that better visualization of the plaques is obtained; that a variety of stains, including fluorescence, can be used; and that the shelf life of the resulting plaques system is well over a year.
Zolkov
Chen
C
Faculty of Civil and Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion, Haifa 32000, Israel.
Avnir
David
D
Armon
Robert
R
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D005453:Fluorescence; D005782:Gels; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000254:growth & development / Q000821:virology*; D008422:Materials Testing / Q000379:methods*; D008751:Methylene Blue; D017955:Poliovirus / Q000254:growth & development* / Q000302:isolation & purification; D012821:Silanes; D013499:Surface Properties; D010948:Viral Plaque Assay / Q000379:methods*; D014773:Virology / Q000379:methods
D005782:Gels; D012821:Silanes; D008751:Methylene Blue