Construction and performance test of a multiplexed multistage (MS(n)) time-of-flight mass spectrometer.
A time-of-flight mass spectrometer equipped with two reflectrons was constructed for multiplexed photodissociation tandem mass spectrometry of peptide ions generated by matrix-assisted laser desorption ionization. A linear reflectron was used for high-resolution selection of a precursor ion while a quadratic reflectron was used for product ion analysis. With the photoexcitation of a precursor ion inside a cell floated at high voltage, information (MS(3)) on intermediate ions generating a particular product ion was obtained. Fully multiplexed detection resulted in good MS(3) signal levels. Use of the quadratic reflectron allowed intermediate ion mass determination within 4 Da. The possibility of further extending the technique and its analytical potential are discussed.
Shin
Young Sik
YS
Department of Chemistry, Seoul National University, Seoul 151-742, Korea.
Moon
Jeong Hee
JH
Kim
Myung Soo
MS
D006801:Humans; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*
D010446:Peptide Fragments