A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples.
A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Rickettsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically.
Svendsen
Claus Bo
CB
Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark. clv@ssi.dk <clv@ssi.dk>
Boye
Mette
M
Struve
Carsten
C
Krogfelt
Karen A
KA
D001431:Bacteriological Techniques; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012281:Rickettsia / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S