title: Effect of lipid extracted from tsao-ko (Amomum tsao-ko Crevost et Lemaire) on digestive enzyme activity, antioxidant activity, plasma and liver lipids, and blood glucose levels of mice.
abstract: Lipids extracted from tsao-ko were separated into three fractions with silica gel column chromatography and fed to mice (3 mo old) for 90 d to clarify their inhibitory activity on digestive enzyme activity. The diets contained the following: control--no tsao-ko, 0.05% total lipid of tsao-ko (TL), 0.0109% chloroform fraction (CF), 0.0245% acetone fraction (AF), or 0.00365% methanol fraction (MeF). Although CF and AF slightly inhibited the activities of alpha-glucosidase, alpha-amylase, and lipase, intakes of these fractions had little influence on plasma and liver lipid concentrations when compared with the control diet. MeF did not inhibit alpha-glucosidase but had DPPH radical scavenging activity and the mice fed this fraction had the most marked reduction in plasma glucose and TBARS concentrations compared with the other diet groups. These results suggest that the fat-soluble polar components of tsao-ko contain an active component that might be associated with decreased plasma glucose and TBARS concentrations in mice.
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Longquan
authors.initials: L
authors.lastname: Yu
authors.forename: Nobuya
authors.initials: N
authors.lastname: Shirai
authors.forename: Hiramitsu
authors.initials: H
authors.lastname: Suzuki
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Hosono
authors.forename: Yoshijiro
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kajiwara
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takatori
mesh_terms: D035801:Amomum / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism*; D001823:Body Composition / Q000187:drug effects; D004064:Digestive System / Q000201:enzymology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000302:isolation & purification / Q000494:pharmacology; D008049:Lipase / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010936:Plant Extracts / Q000494:pharmacology*; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances; D000516:alpha-Amylases / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D016166:Free Radical Scavengers; D000960:Hypolipidemic Agents; D008055:Lipids; D010936:Plant Extracts; D010938:Plant Oils; D017392:Thiobarbituric Acid Reactive Substances; D008049:Lipase; D000516:alpha-Amylases; D000520:alpha-Glucosidases