A cation-regulated and proton gradient-dependent cation transporter from Chlamydomonas reinhardtii has a role in calcium and sodium homeostasis.
The CrCAX1 gene encoding a Ca2+/H+ and Na+/H+ exchanger was cloned and characterized from the unicellular green alga Chlamydomonas reinhardtii to begin to understand the mechanisms of cation homeostasis in this model organism. CrCAX1 was more closely related to fungal cation exchanger (CAX) genes than those from higher plants but has structural characteristics similar to plant Ca2+/H+ exchangers including a long N-terminal tail. When CrCAX1-GFP was expressed in Saccharomyces cerevisiae, it localized at the vacuole. CrCAX1 could suppress the Ca2+-hypersensitive phenotype of a yeast mutant and mediated proton gradient-dependent Ca2+/H+ exchange activity in vacuolar membrane vesicles. Ca2+ transport activity was increased following N-terminal truncation of CrCAX1, suggesting the existence of an N-terminal auto-regulatory mechanism. CrCAX1 could also provide tolerance to Na+ stress when expressed in yeast or Arabidopsis thaliana because of Na+/H+ exchange activity. This Na+/H+ exchange activity was not regulated by the N terminus of the CrCAX1 protein. A subtle tolerance by CrCAX1 in yeast to Co2+ stress was also observed. CrCAX1 was transcriptionally regulated in Chlamydomonas cells grown in elevated Ca2+ or Na+. This study has thus uncovered a novel eukaryotic proton-coupled transporter, CrCAX1, that can transport both monovalent and divalent cations and that appears to play a role in cellular cation homeostasis by the transport of Ca2+ and Na+ into the vacuole.
Pittman
Jon K
JK
Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, United Kingdom. Electronic address: jon.pittman@manchester.ac.uk.
Edmond
Clare
C
Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, United Kingdom.
Sunderland
Paul A
PA
Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, United Kingdom.
Bray
Clifford M
CM
Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, United Kingdom.
D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D002412:Cations / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology*; D017136:Ion Transport / Q000502:physiology; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary / Q000502:physiology; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D014617:Vacuoles / Q000235:genetics / Q000378:metabolism
D020418:Algal Proteins; D017920:Antiporters; D027682:Cation Transport Proteins; D002412:Cations; D015800:Protozoan Proteins; C027614:calcium-hydrogen antiporters; D012964:Sodium; D002118:Calcium