The geometric control of E14 and R1 mouse embryonic stem cell pluripotency by plasma polymer surface chemical gradients.
Plasma polymer surfaces were fabricated such that the cell response to a range of carboxylic acid concentrations on a single sample could be investigated. Surface chemical gradients from hydrophobic plasma polymerised octadiene (OD) to a more hydrophilic plasma polymerised acrylic acid (AA) were formed on glass coverslips. Surface characterisation of the chemical gradients was performed using X-ray photoelectron spectroscopy to determine elemental composition. Following culture of E14 and R1 mouse embryonic stem cells (mES) in differing culture media, cell pluripotency was determined by alkaline phosphatase staining. The results demonstrate that for these cell lines the capacity for self-renewal is maintained if the cells are restricted in their spreading to <120 microm2.
Wells
Nicola
N
School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, UK.
Baxter
Melissa A
MA
Turnbull
Jeremy E
JE
Murray
Patricia
P
Edgar
David
D
Parry
Kristina L
KL
Steele
David A
DA
Short
Robert D
RD
D000179:Acrylates / Q000494:pharmacology*; D000268:Adhesiveness / Q000187:drug effects; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects*; D011108:Polymers / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects
D000179:Acrylates; D003470:Culture Media; D011108:Polymers; C036658:acrylic acid