title: Temperature influences the expression of fimbriae and flagella in Hafnia alvei strains: an immunofluorescence study.
abstract: Hafnia alvei, a Gram negative bacillus related to the Enterobacteriaceae family, is considered an opportunistic pathogen of several animal species and humans. In this communication, we describe fimbrial-like structures from different strains of H. alvei that cannot be easily ascribed to any of the previously reported fimbrial types in this species (type I or type III). Polymerase chain reaction (PCR) and immunofluorescence assays were carried out to study fimbriae and flagella in H. alvei strains isolated from different sources. No correlation between the results obtained by PCR and those obtained by phenotypic methods were found, and the antibodies used gave cross or different recognition patterns of the surface structures present in these strains. We report as well that strain and growth temperature influence fimbriation and expression of flagella in human and animal isolates of H. alvei. This study also indicates that the absence of fimbriae have a significant positive influence on the initial adhesion of H. alvei to human epithelial cells.
authors.affiliation: University Institute of Animal Health, IUSA, Universidad de Las Palmas de Gran Canaria, 35416 Arucas, Las Palmas, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Padilla
authors.forename: F
authors.initials: F
authors.lastname: Acosta
authors.forename: J A
authors.initials: JA
authors.lastname: García
authors.forename: F
authors.initials: F
authors.lastname: Real
authors.forename: Jose R
authors.initials: JR
authors.lastname: Vivas
mesh_terms: D001422:Bacterial Adhesion; D010861:Fimbriae, Bacterial / Q000502:physiology / Q000648:ultrastructure*; D005407:Flagella / Q000502:physiology / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D020611:Hafnia alvei / Q000502:physiology* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D013696:Temperature