title: Peptidase substrates via global peptide profiling.
abstract: Peptide metabolism is a complex process that involves many proteins working in concert. Mass spectrometry-based global peptide profiling of mice lacking dipeptidyl peptidase 4 (DPP4) identified endogenous DPP4 substrates and revealed an unrecognized pathway during proline peptide catabolism that interlinks aminopeptidase and DPP4 activities. Together, these studies elucidate specific aspects of DPP4-regulated metabolism and, more generally, highlight the utility of global peptide profiling for studying peptide metabolism in vivo.
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.
authors.forename: Debarati M
authors.initials: DM
authors.lastname: Tagore
authors.forename: Whitney M
authors.initials: WM
authors.lastname: Nolte
authors.forename: John M
authors.initials: JM
authors.lastname: Neveu
authors.forename: Roberto
authors.initials: R
authors.lastname: Rangel
authors.forename: Liliana
authors.initials: L
authors.lastname: Guzman-Rojas
authors.forename: Renata
authors.initials: R
authors.lastname: Pasqualini
authors.forename: Wadih
authors.initials: W
authors.lastname: Arap
authors.forename: William S
authors.initials: WS
authors.lastname: Lane
authors.forename: Alan
authors.initials: A
authors.lastname: Saghatelian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018819:Dipeptidyl Peptidase 4 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054873:Dipeptidyl-Peptidase IV Inhibitors; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D017510:Protein Folding
chemical_list: D054873:Dipeptidyl-Peptidase IV Inhibitors; D018819:Dipeptidyl Peptidase 4