title: Changes in muscle fiber size and in the composition of myosin heavy chain isoforms of rabbit extraocular rectus muscle following recession surgery.
abstract: OBJECTIVE
  To assess the changes in the size of muscle fibers and the composition of myosin heavy chain (MyHC) isoforms in the global layer (GL) and the orbital layer (OL) of rabbit rectus extraocular muscle (EOM) after recession.
  
  
  METHODS
  The right superior rectus muscles of two rabbits were harvested at 3 days or 1, 2, or 4 weeks after recession (eight rabbits in total). At each time point, one muscle was used for measuring the cross-sectional area of the muscle fibers and the other for identifying the composition of MyHC. The right superior rectus muscles of three additional naïve rabbits were used as controls.
  
  
  RESULTS
  The mean cross-sectional area of the OL fibers did not change significantly. However, that of the GL fibers significantly decreased at 3 days (P<0.001) and 1 week (P=0.024) postoperatively, and increased thereafter to reach the control levels at 2 and 4 weeks postoperatively. Three days after surgery, the total MyHC content and the proportion of type IIb MyHC (MyHCIIb) plus EOM-specific MyHC (MyHCeom) decreased and remained at its lower level for 4 weeks.
  
  
  CONCLUSIONS
  Transient atrophy and regeneration were observed only in the GL, and the changes in the MyHCIIb plus MyHCeom appeared to be related to these changes.
authors.affiliation: Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Park
authors.affiliation: Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Yun Taek
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Sun A
authors.initials: SA
authors.lastname: Kim
authors.affiliation: Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea. syoh@skku.edu.|Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Kangnam-ku, Seoul, 135-710, Republic of Korea. syoh@skku.edu.
authors.forename: Sei Yeul
authors.initials: SY
authors.lastname: Oh
mesh_terms: D000818:Animals; D001284:Atrophy; D005260:Female; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000473:pathology*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D009801:Oculomotor Muscles / Q000378:metabolism / Q000601:surgery*; D013508:Ophthalmologic Surgical Procedures; D020033:Protein Isoforms / Q000378:metabolism; D011817:Rabbits
chemical_list: D020033:Protein Isoforms; D018995:Myosin Heavy Chains