Avian pathogenic Escherichia coli bind fibronectin and laminin.
Avian colisepticemia frequently occurs after respiratory tract damage, the primary site for infection allows bacteria to encounter an exposed basement membrane, where laminin and fibronectin are important components. We investigated the ability of an isolate of avian pathogenic Escherichia coli to bind fibronectin and laminin. Using Far-western dot blot analysis, we demonstrated the ability of this microorganism to bind basement membrane proteins fibronectin and laminin. Results from an ELISA-based approach indicate that the binding to these membrane proteins was bacterial-dose dependent. Furthermore, two specific E. coli polypeptides, of 32 kDa and 130 kDa, reacted with laminin and fibronectin, respectively. Further evaluation of these potential bacterial adhesins may provide insights into the pathogenesis of colibacillosis.
Ramírez
Rosa María
RM
Departamento de Enfermedades Infecciosas, Unidad Académica de Biología Experimental, Universidad Autónoma de Zacatecas, Calzada de la Revolución Mexicana s/n, Guadalupe Zac, 98600, México.
Almanza
Yolanda
Y
González
Rafael
R
García
Santos
S
Heredia
Norma
N
D000818:Animals; D001422:Bacterial Adhesion; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000378:metabolism* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D005109:Extracellular Matrix / Q000378:metabolism / Q000382:microbiology; D005353:Fibronectins / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism*; D011201:Poultry Diseases / Q000378:metabolism / Q000382:microbiology*
D005353:Fibronectins; D007797:Laminin