title: Identification of novel splice variants of the Arabidopsis DCL2 gene.
abstract: In Arabidopsis thaliana, Dicer-like protein 2 (DCL2) cleaves double-stranded virus RNA, playing an essential role in the RNA interference pathway. Here, we describe three alternative splicing (AS) forms of AtDCL2: in one, both intron 8 and intron 10 are retained in the mRNA, in second only intron 8 is retained and in the third no intron is retained, but there is a deletion of 56 nucleotides at the end of exon 10. These splicing forms are present in stems and leaves at different development stages. AS was also detected in DCL2 of Brassica rapa, where intron 9, but not intron 8 or intron 10, was retained suggesting that AS may be a common phenomenon in cruciferous plant DCL2s. The retained introns and sequence deletions detected in AtDCL2 changed the reading frame and produced premature terminal codons. The AS forms appeared to be substrates of nonsense-mediated decay of mRNA.
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Jiejun
authors.initials: J
authors.lastname: Peng
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Lu
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Hongying
authors.initials: H
authors.lastname: Zheng
authors.forename: Hairu
authors.initials: H
authors.lastname: Chen
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Adams
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D029741:Brassica rapa / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics*; D005091:Exons / Q000235:genetics; D007438:Introns / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D018547:Plant Stems / Q000235:genetics; D012326:RNA Splicing / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; C511919:DCL2 protein, Arabidopsis; D043244:Ribonuclease III