title: Detection of herpesvirus and adenovirus co-infections with diagnostic DNA-microarrays.
abstract: In immunocompromised patients, the diagnosis of infections with herpesviruses and adenoviruses relies mainly on PCR amplification of viral genomic DNA from clinical samples. In the case of co-infections with two or more viruses, single amplification of viral DNA from clinical samples has proven to be time-consuming and expensive, hampering the efficient diagnosis and therapy of viral co-infections. In this study, a diagnostic DNA-microarray allowing simultaneous detection of herpes simplex virus types 1 and 2 (HSV 1/2), varicella zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus-6 types A and B (HHV-6 A/B), and adenovirus in clinical samples was developed and validated. The assay displays a high analytical sensitivity (10genomeequivalents(GE)/reaction) and specificity, being cost-effective and time-saving. Because the DNA-microarray uses the same analytical conditions as real-time quantitative PCR, it can be used as a screening device for multiple viral infections, followed by selective viral load measurement depending on the clinical context. Those features make the DNA-microarray an attractive device for the management of viral infections in immunocompromised patients.
authors.affiliation: The Calicilab, Institut für Virologie, Medizinische Fakultät Carl Gustav Carus, Dresden, Germany.
authors.forename: René
authors.initials: R
authors.lastname: Müller
authors.forename: Anette
authors.initials: A
authors.lastname: Ditzen
authors.forename: Kristin
authors.initials: K
authors.lastname: Hille
authors.forename: Markus
authors.initials: M
authors.lastname: Stichling
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
authors.forename: Thomas
authors.initials: T
authors.lastname: Illmer
authors.forename: Gerhard
authors.initials: G
authors.lastname: Ehninger
authors.forename: Jacques
authors.initials: J
authors.lastname: Rohayem
mesh_terms: D000258:Adenovirus Infections, Human / Q000150:complications / Q000175:diagnosis / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification / Q000502:physiology; D006564:Herpesviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000150:complications / Q000175:diagnosis / Q000821:virology; D006801:Humans; D016867:Immunocompromised Host; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D033581:Stem Cell Transplantation / Q000009:adverse effects; D019562:Viral Load
chemical_list: D004279:DNA, Viral