Derivation of human embryonic stem cells by immunosurgery.
The ability of human embryonic stem cells to self-renew and differentiate into all cell types of the body suggests that they hold great promise for both medical applications and as a research tool for addressing fundamental questions in development and disease. Here, we provide a concise, step-by-step protocol for the derivation of human embryonic stem cells from embryos by immunosurgical isolation of the inner cell mass.
Chen
Alice E
AE
Dept. of Molecular and Cellular Biology, Harvard University, USA. aechen@mcb.harvard.edu
Melton
Douglas A
DA
D001755:Blastocyst / Q000166:cytology*; D002455:Cell Division; D002469:Cell Separation / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology; D006801:Humans; D007158:Immunologic Techniques