title: Microseparation techniques for the study of the enantioselectivity of drug-plasma protein binding.
abstract: Stereoselectivity in protein binding can have a significant effect on the pharmacokinetic and pharmacodynamic properties of chiral drugs. The investigation of enantioselectivity of drugs in their binding with human plasma proteins and the identification of the molecular mechanisms involved in the stereodiscrimination by the proteins represent a great challenge for clinical pharmacology. In this review, the separation techniques used for enantioselective protein binding experiments are described and compared. An overview of studies on enantiomer-protein interactions, enantiomer-enantiomer interactions as well as chiral drug-drug interactions, including allosteric effects, is presented. The contribution of individual plasma proteins to the overall enantioselective binding and the animal species variability in drug-plasma protein binding stereoselectivity are reviewed.
authors.affiliation: Departamento de Química Analítica, Universitat de València, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Escuder-Gilabert
authors.forename: María Amparo
authors.initials: MA
authors.lastname: Martínez-Gómez
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Villanueva-Camañas
authors.forename: Salvador
authors.initials: S
authors.lastname: Sagrado
authors.forename: María José
authors.initials: MJ
authors.lastname: Medina-Hernández
mesh_terms: D000495:Allosteric Site; D001798:Blood Proteins / Q000378:metabolism*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D019075:Electrophoresis, Capillary; D006801:Humans; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011485:Protein Binding; D013045:Species Specificity; D013237:Stereoisomerism
chemical_list: D001798:Blood Proteins; D004364:Pharmaceutical Preparations