Relative strengths of the class 1 integron promoter hybrid 2 and the combinations of strong and hybrid 1 with an active p2 promoter.
The relative strengths of the uncommon promoters hybrid 2, hybrid 1 with an active P2 promoter (hybrid 1+P2), and strong+P2, which drive transcription of resistance genes in class 1 integrons, were evaluated using bla(GES-1) as a reporter gene cassette. Hybrid 2 was stronger than hybrid 1. Coupling P2 with the strong promoter and with hybrid 1 caused a measurable increase in GES-1 expression.
Papagiannitsis
Costas C
CC
Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
Tzouvelekis
Leonidas S
LS
Miriagou
Vivi
V
D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
D047090:beta-Lactams; D001618:beta-Lactamases