Selection of an effective red-pigment producing Monascus pilosus by efficient transformation with aurintricarboxylic acid.
The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction-enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.
Chen
Yi-Pei
YP
Department of Biological Science and Technology, National Chiao Tung University, Taiwan.
Chen
I-Ching
IC
Hwang
Ing-Er
IE
Yuan
Gwo-Fang
GF
Liaw
Li-Ling
LL
Tseng
Ching-Ping
CP
D000818:Animals; D001312:Aurintricarboxylic Acid / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006801:Humans; D032741:Monascus / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D010858:Pigmentation; D010957:Plasmids / Q000235:genetics; D011092:Polyethylene Glycols / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects; D014170:Transformation, Genetic / Q000187:drug effects*
D011092:Polyethylene Glycols; D001312:Aurintricarboxylic Acid; D004262:DNA Restriction Enzymes; D002122:Calcium Chloride