Optical properties of amyloid stained by Congo red: history and mechanisms.
Amyloid stained by Congo red has striking optical properties that generally have been poorly described and inadequately explained, although they can be understood from principles of physical optics. Molecules of Congo red are orientated on amyloid fibrils, and so the dye becomes dichroic and birefringent. The birefringence varies with wavelength in accordance with a fundamental property of all light-transmitting materials called anomalous dispersion of the refractive index around an absorption peak. The combination of this and absorption of light, with modification by any additional birefringence in the optical system, explains the various colours that can be seen in Congo red-stained amyloid between crossed polariser and analyser, and also when the polariser and analyser are progressively uncrossed. These are called anomalous colours.
Howie
Alexander J
AJ
Department of Pathology, University College London, London, UK. a.j.howie@medsch.ucl.ac.uk
Brewer
Douglas B
DB
D000682:Amyloid / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D001718:Birefringence; D002942:Circular Dichroism; D003116:Color; D004396:Coloring Agents; D003224:Congo Red; D049672:History, 19th Century; D049673:History, 20th Century; D006801:Humans; D008859:Microscopy, Polarization; D012031:Refractometry; D013194:Staining and Labeling / Q000266:history
D000682:Amyloid; D004396:Coloring Agents; D003224:Congo Red