Impact of inactivated extracellular proteases on the modified flagellin type III secretion pathway of Bacillus halodurans.
The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (Deltahag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases.
Berger
Eldie
E
CSIR Biosciences, Box 395, Pretoria 0001, South Africa.
du Plessis
Erika
E
Gerber
Isak
I
Crampton
Michael
M
Nxumalo
Nolwandle
N
Louw
Maureen
M
D001407:Bacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D005408:Flagellin / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D026901:Membrane Transport Proteins / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D011993:Recombinant Fusion Proteins; D005408:Flagellin; C076733:FlaD protein, Bacteria; D010447:Peptide Hydrolases