Alterations of AQP2 expression in trigeminal ganglia in a murine inflammation model.
Aquaporins (AQPs) are small membrane channel proteins involved in osmoregulation. To date, only AQP1, AQP2, AQP4 and AQP9 have been found in the nervous system. Generally, they are involved in water movement in nervous tissue, nevertheless, recent data would suggest the involvement of AQPs in neurotransmission. In this work, we have evaluated the expression of AQP1 and AQP2 in the trigeminal ganglia of mice in an animal model of perioral acute inflammatory pain using immunohistochemistry and immunoblotting analysis. Our data have shown for the first time, the alteration of AQP2 expression in trigeminal ganglia in acute inflammatory pain showing increased and intracellular redistribution of AQP2 mainly in small-sized neurons and Schwann cells. Apart from this, the AQP1 expression remained unaltered. On the whole, these data support the hypothesis that AQP2 is involved in pain transmission in the peripheral nervous system.
Borsani
Elisa
E
Department of Biomedical Sciences and Biotechnologies, University of Brescia, Viale Europa 11, 25123 Brescia, Italy.
Bernardi
Simona
S
Albertini
Roberta
R
Rezzani
Rita
R
Rodella
Luigi F
LF
D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism; D051399:Aquaporin 2 / Q000378:metabolism*; D004195:Disease Models, Animal; D005557:Formaldehyde; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism; D013997:Time Factors; D012668:Trigeminal Ganglion / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
C496021:Aqp1 protein, mouse; C496024:Aqp2 protein, mouse; D051399:Aquaporin 2; D051398:Aquaporin 1; D005557:Formaldehyde