title: [Determination of organic acids in dental plaque with high performance liquid chromatography].
abstract: OBJECTIVE
  To establish determination method of formic acid, lactic acid, acetic acid and succinic acid in dental plaque with high performance liquid chromatography (HPLC).
  
  
  METHODS
  After the samples were centrifuged, 2 microL supernatant was transferred to a 1 mL centrifuge tube and diluted in water, then was determined with HPLC. The mixture of phosphate buffer and methanol (97:3) as mobile phase throughout the experiment. The determination of organic acid was performed on Phenomenex C18 column and at their maximum absorption wave.
  
  
  RESULTS
  The linear ranges of formic acid, lactic acid, acetic acid and succinic acid were 0.110-500, 0.049-500, 0.047-500, 0.084-500 microg/mL. The detection limits were 0.110, 0.049, 0.047, 0.084 microg/mL. The relative standard derivation were 9.5%, 7.9%, 4.3%, 4.2%. The average recoveries of samples were 82%-112%, 82%-102.5%, 90%-115%, 80%-110%.
  
  
  CONCLUSIONS
  The method was simple, quick and adapt for analysis of organic acid in dental plaque.
authors.affiliation: Dept. of Sanitary Technology, West China College of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Zou
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Zeng
authors.forename: Ci-ling
authors.initials: CL
authors.lastname: Yan
authors.forename: Wan-chun
authors.initials: WC
authors.lastname: Zhu
authors.forename: Ji-yao
authors.initials: JY
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003773:Dental Plaque; D005561:Formates
chemical_list: D005561:Formates; C030544:formic acid