Simultaneous detection and quantitation of highly water-soluble herbicides in serum using ion-pair liquid chromatography-tandem mass spectrometry.
We report the simultaneous screening of highly polar, water-soluble, and less-volatile herbicides, including glyphosate, glufosinate, paraquat, and diquat, in serum using liquid chromatography-mass spectrometry. The herbicides were separated by solid-phase extraction using a Strata-XC cartridge. A heptafluorobutyric acid solution was chosen as the mobile phase for ion-pair liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The serum specimens were screened for the presence of the herbicides at the following concentrations: 5 ng/mL for glyphosate, 2 ng/mL for glufosinate, 1 ng/mL for diquat, and 5 ng/mL for paraquat. This is the first report on the simultaneous detection of these compounds.
Wang
Kuang-Chuan
KC
College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan, ROC.
Chen
Shih-Ming
SM
Hsu
Jung-Fa
JF
Cheng
Sheaw-Guey
SG
Lee
Ching-Kuo
CK
D002853:Chromatography, Liquid / Q000379:methods*; D005466:Fluorocarbons / Q000737:chemistry; D006540:Herbicides / Q000097:blood* / Q000737:chemistry; D006801:Humans; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014867:Water / Q000737:chemistry
D005466:Fluorocarbons; D006540:Herbicides; D014867:Water; C033094:perfluorobutyric acid