Electrochemical detection of blood alcohol concentration using a disposable biosensor based on screen-printed electrode modified with Nafion and gold nanoparticles.
BACKGROUND
Blood alcohol determination plays an important role in laboratory medicine and forensic medicine. Nowadays, many methods are being used for alcohol measurement, but these methods are time-consuming and complex to perform laborious sample pre-treatment. The disposable amperometric biosensor, due to its portability, low cost and potential for fabrication, should be readily applicable for blood alcohol determination.


METHODS
The biosensor was fabricated by immobilizing alcohol dehydrogenase and nicotinamide adenine dinucleotide coated by Nafion combined with gold nanoparticles onto the surface of screen-printed electrode modified with Meldola's blue. Evaluations of biosensor performance were performed according to the relevant National Committee for Clinical Laboratory Standards standard.


RESULTS
The biosensor response for serum alcohol presents good linearity, precision, stability, accuracy, and specificity.


CONCLUSIONS
The biosensor exhibits the capability of detecting blood alcohol concentration in the clinical laboratory and in forensic medicine, unnecessarily performing laborious sample pre-treatment.
Luo
Peng
P
Department of Laboratory Medicine, Chongqing Medical University, Chongqing, China.
Xie
Guoming
G
Liu
Yi
Y
Xu
Huajian
H
Deng
Shixiong
S
Song
Fangzhou
F
D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000032:analysis / Q000097:blood*; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D010078:Oxazines / Q000737:chemistry; D015203:Reproducibility of Results; D013696:Temperature
D004800:Enzymes, Immobilized; D005465:Fluorocarbon Polymers; D010078:Oxazines; D009243:NAD; C040402:perfluorosulfonic acid; D000431:Ethanol; C005559:Meldola blue; D006046:Gold; D000426:Alcohol Dehydrogenase