Lipocalin-2 is induced by interleukin-1beta in murine adipocytes in vitro.
Lipocalin-2 (Lcn2) has recently been isolated as an adipocyte-secreted acute phase reactant that plays a role in insulin resistance, obesity, and atherosclerotic disease. In the current study, we determined regulation of Lcn2 by the proinflammatory and insulin resistance-inducing cytokine interleukin (IL)-1beta in 3T3-L1 and brown adipocytes by relative real-time reverse transcription-polymerase chain reaction. Interestingly, IL-1beta dramatically induced Lcn2 mRNA in both adipocyte models. Furthermore, Lcn2 protein secretion was dramatically upregulated in 3T3-L1 adipocytes after 24 h of IL-1beta treatment. Experiments using pharmacological inhibitors indicated that IL-1beta-induced Lcn2 expression is mediated via nuclear factor kappaB and janus kinase 2. Taken together, our results show an upregulation of Lcn2 by IL-1beta in fat cells implicating a potential role of this adipocyte-secreted acute phase reactant in the development of insulin resistance, obesity, and associated disorders including cardiovascular disease.
Sommer
Grit
G
Department of Internal Medicine III, University of Leipzig, 04103 Leipzig, Germany.
Weise
Sebastian
S
Kralisch
Susan
S
Lossner
Ulrike
U
Bluher
Matthias
M
Stumvoll
Michael
M
Fasshauer
Mathias
M
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D053583:Interleukin-1beta / Q000494:pharmacology*; D053614:Janus Kinase 2 / Q000378:metabolism; D054834:Lipocalins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
D053583:Interleukin-1beta; D054834:Lipocalins; D016328:NF-kappa B; D012333:RNA, Messenger; D053614:Janus Kinase 2