title: Micro-dissection of rat brain for RNA or protein extraction from specific brain region.
abstract: Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.
authors.affiliation: Laboratory of Neurodegenerative Diseases, Department of Anatomy, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
authors.forename: Kin
authors.initials: K
authors.lastname: Chiu
authors.forename: Wui Man
authors.initials: WM
authors.lastname: Lau
authors.forename: Ho Tak
authors.initials: HT
authors.lastname: Lau
authors.forename: Kwok-Fai
authors.initials: KF
authors.lastname: So
authors.forename: Raymond Chuen-Chung
authors.initials: RC
authors.lastname: Chang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000601:surgery; D003342:Corpus Striatum / Q000378:metabolism; D005625:Frontal Lobe / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D042282:Microdissection / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D009830:Olfactory Bulb / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification*; D051381:Rats; D013048:Specimen Handling / Q000379:methods*
chemical_list: D009419:Nerve Tissue Proteins; D012313:RNA