In vivo imaging of neurodegeneration in Caenorhabditis elegans by third harmonic generation microscopy.
In this study, neurodegeneration phenomena were investigated, by performing third harmonic generation imaging measurements on the nematode Caenorhabditis elegans, in vivo. The in vivo, precise identification of the contour of the degenerating neurons in the posterior part of the nematode and the monitoring, in real time, of the progression of degeneration in the worm, through third harmonic generation imaging measurements, were achieved. Femtosecond laser pulses (1028 nm) were utilized for excitation. Thus, the THG image contrast modality comprises a powerful diagnostic tool, providing valuable information and offering new insights into morphological changes and complex developmental processes in live biological specimens.
Gualda
E J
EJ
Institute of Electronic Structure and Laser, Foundation of Research and Technology-Hellas, 71110 Heraklion, Crete, Greece.
Filippidis
G
G
Mari
M
M
Voglis
G
G
Vlachos
M
M
Fotakis
C
C
Tavernarakis
N
N
D000818:Animals; D017173:Caenorhabditis elegans / Q000033:anatomy & histology* / Q000254:growth & development*; D008853:Microscopy / Q000379:methods*; D009024:Morphogenesis; D009420:Nervous System / Q000033:anatomy & histology* / Q000254:growth & development*