Suitability of a magnetic particle immunoassay for the analysis of PBDEs in Hawaiian euryhaline fish and crabs in comparison with gas chromatography/electron capture detection-ion trap mass spectrometry.
A gas chromatograph/electron capture detector-ion trap mass spectrometer (GC/ECD-ITMS) was used for the determination of polybrominated diphenyl ethers (PBDEs) in euryhaline fish and crabs. GC/ECD-ITMS results showed that average recoveries from the spiked fish samples are in a range of 58-123% with relative standard deviations (RSDs) of 5-19%. PBDE concentrations obtained from GC/ECD-ITMS ranged from 28 ng/g to 1845 ng/g lipid weight (lw) in all aquatic species collected from Hawaiian brackish waters. The general BDE congener concentration profile observed in this study is BDE-47>BDE-100>BDE-154>BDE-99>BDE-153>BDE-28>BDE-183. The ELISA results expressed as BDE-47 equivalents correlated well with those of GC/ECD-ITMS, with a correlation coefficient (R(2)=0.68) and regression coefficient (slope=0.82). Comparison of ELISA with GC/ECD-ITMS results demonstrated that ELISA provides a timely and cost-effective method to screen PBDEs in fish and crab samples.
Xu
Ting
T
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Cho
Il Kyu
IK
Wang
Dongli
D
Rubio
Fernando M
FM
Shelver
Weilin L
WL
Gasc
Anne M E
AM
Li
Ji
J
Li
Qing X
QX
D000818:Animals; D003386:Brachyura / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D005506:Food Contamination / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D055768:Halogenated Diphenyl Ethers / Q000032:analysis*; D008280:Magnetics; D014874:Water Pollutants, Chemical / Q000032:analysis*
D055768:Halogenated Diphenyl Ethers; D014874:Water Pollutants, Chemical