Experimental infection studies of UK Culicoides species midges with bluetongue virus serotypes 8 and 9.
This paper describes a rapid, standardised method for testing the susceptibility to bluetongue virus (BTV) of northern Palaearctic Culicoides species midges that can be used to assess the competence of both field-caught and laboratory-infected midges. The method has been used to show that Culicoides scoticus can replicate btv serotype 8 and BTV serotype 9 strains to more than 3 log(10) TCID50/midge, the first evidence of the potential of this species to transmit BTV.
Carpenter
S
S
Institute for Animal Health (IAH) Pirbright, Ash Road, Woking, Surrey GU24 0BN, UK.
McArthur
C
C
Selby
R
R
Ward
R
R
Nolan
D V
DV
Luntz
A J Mordue
AJ
Dallas
J F
JF
Tripet
F
F
Mellor
P S
PS
D000818:Animals; D001820:Bluetongue virus / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology*; D002519:Ceratopogonidae / Q000145:classification / Q000821:virology*; D007303:Insect Vectors / Q000145:classification / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary; D013045:Species Specificity; D006113:United Kingdom; D014779:Virus Replication