Light scattering by neutrophils: model, simulation, and experiment.
We studied the elastic light-scattering properties of human blood neutrophils, both experimentally and theoretically. The experimental study was performed with a scanning flow cytometer measuring the light-scattering patterns (LSPs) of individual cells over an angular range of 5-60 deg. We determined the absolute differential light-scattering cross sections of neutrophils. We also proposed an optical model for a neutrophil as a sphere filled by small spheres and prolate spheroids that correspond to granules and segmented nucleus, respectively. This model was used in simulations of LSPs using the discrete dipole approximation and different compositions of internal organelles. A comparison of experimentally measured and simulated LSPs gives a good qualitative agreement in LSP shape and quantitative agreement in overall magnitude of the differential light-scattering cross section.
Orlova
Darya Yu
DY
Institute of Chemical Kinetics and Combustion, Institutskaya 3, 630090, Novosibirsk, Russia.
Yurkin
Maxim A
MA
Hoekstra
Alfons G
AG
Maltsev
Valeri P
VP
D002478:Cells, Cultured; D003198:Computer Simulation; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D008955:Models, Cardiovascular; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods*