Simultaneous multiwavelength laminar optical tomography.
Spatially resolved reflectance measurements can be used to characterize the depth-resolved optical properties of superficial tissues. However, until now, rapid acquisition of multiwavelength data has been hindered by multiplexing problems. We report on a novel multiwavelength laminar optical tomography system capable of acquiring data from multiple source-detector separations at three wavelengths simultaneously. Such data can allow in vivo depth-resolved spectroscopic imaging of absorbers, such as oxy- and deoxyhemoglobin, or of multiple fluorophores, that is unaffected by motion artifacts at frame rates exceeding 100 Hz. The system design and phantom validation studies are presented.
Burgess
Sean A
SA
Laboratory for Functional Optical Imaging, Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA. sab2161@columbia.edu
Bouchard
Matthew B
MB
Yuan
Baohong
B
Hillman
Elizabeth M C
EM
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation*