title: Fibulin-5 expression is decreased in women with anterior vaginal wall prolapse.
abstract: The aim of this study was to compare fibulin-5 expression in women with and without anterior vaginal wall prolapse. Vaginal tissues were sampled in a standardized fashion from women with (n = 12) or without (n = 10) anterior vaginal wall prolapse. Quantitative real-time polymerase chain reaction was performed to measure mRNA levels of fibulin-5 (FIB-5). FIB-5 protein expression was assessed by immunohistochemistry. There were no significant differences in demographic data between the two groups. FIB-5 mRNA expression was significantly decreased in women with anterior vaginal wall prolapse compared to women without prolapse [(FIB-5 mean +/- SD mRNA expression in relative units) 0.01 +/- 0.01 vs. 0.09 +/- 0.14, P = 0.04]. Fibulin-5 staining intensity was diminished in women with prolapse compared to women without prolapse [intensity score, median (range), 1 (1-2) vs. 3 (2-3), P = 0.04]. Fibulin-5 expression is decreased in vaginal biopsies from women with prolapse. Changes in fibulin expression may play a role in the development of pelvic organ prolapse.
authors.affiliation: Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics and Gynecology (D-50), Miller School of Medicine, University of Miami-Jackson Memorial Hospital, P.O. Box 016960, Miami, FL 33010, USA. ptakacs@med.miami.edu
authors.forename: Peter
authors.initials: P
authors.lastname: Takacs
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nassiri
authors.forename: Anita
authors.initials: A
authors.lastname: Viciana
authors.forename: Keith
authors.initials: K
authors.lastname: Candiotti
authors.forename: Alessia
authors.initials: A
authors.lastname: Fornoni
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Medina
mesh_terms: D001706:Biopsy; D016022:Case-Control Studies; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007044:Hysterectomy; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D014596:Uterine Prolapse / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D014621:Vagina / Q000378:metabolism* / Q000473:pathology
chemical_list: D016326:Extracellular Matrix Proteins; C492373:FBLN5 protein, human; D012333:RNA, Messenger