title: Role of viral induced vascular endothelial growth factor (VEGF) production in pleural effusion and malignant mesothelioma.
abstract: Vascular endothelial growth factor (VEGF) plays a key role in formation of pleural effusions and in tumorigenesis and progression of malignant mesothelioma. Mesothelial cells (MC) express the viral receptors Toll-like receptor 3 (TLR3), RIG-I and MDA5. Activation of these receptors by viral RNA exemplified by poly(I:C) RNA leads to a time- and dose-dependent increase of mesothelial VEGF synthesis. To show the specific effect of viral receptors knockdown experiments with siRNA for TLR3, RIG-I and MDA5 were performed. This finding of viral induced mesothelial VEGF synthesis may indicate a novel link between viral infections and formation of pleural effusions and progression of malignant mesothelioma.
authors.affiliation: Medical Policlinic, Ludwig-Maximilians-University, Munich, Germany. markus.woernle@med.uni-muenchen.de
authors.forename: Markus
authors.initials: M
authors.lastname: Wörnle
authors.forename: Matthias
authors.initials: M
authors.lastname: Sauter
authors.forename: Kathrin
authors.initials: K
authors.lastname: Kastenmüller
authors.forename: Andrea
authors.initials: A
authors.lastname: Ribeiro
authors.forename: Maximilian
authors.initials: M
authors.lastname: Roeder
authors.forename: Thomas
authors.initials: T
authors.lastname: Mussack
authors.forename: Roland
authors.initials: R
authors.lastname: Ladurner
authors.forename: Thomas
authors.initials: T
authors.lastname: Sitter
mesh_terms: D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D006801:Humans; D008654:Mesothelioma / Q000378:metabolism* / Q000821:virology; D016066:Pleural Effusion, Malignant / Q000378:metabolism* / Q000821:virology; D011070:Poly I-C / Q000494:pharmacology; D034741:RNA, Small Interfering; D012367:RNA, Viral / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D012367:RNA, Viral; D042461:Vascular Endothelial Growth Factor A; D011070:Poly I-C