Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies.
The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.
Guo
Han-Wen
HW
Institute of Biophotonics, National Yang-Ming University, 155 Li-Nong Street, Section 2, Taipei 11221, Taiwan.
Chen
Chien-Tsun
CT
Wei
Yau-Huei
YH
Lee
Oscar K
OK
Gukassyan
Vladimir
V
Kao
Fu-Jen
FJ
Wang
Hsing-Wen
HW
D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009243:NAD / Q000032:analysis*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence / Q000379:methods*
D009243:NAD