Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8alpha and CD28.
The cDNAs of three turkey CD markers, CD4, CD8alpha and CD28, were identified by screening a turkey cDNA library. The coding regions of the chicken and turkey genes are highly conserved, with 91.3-96.1% nucleotide (nt) and 84.2-95.5% amino acid (aa) identity. Identity was less conserved between avian CD markers and their mammalian homologues, ranging from 44.7 to 59.8% and 22.4 to 50.4% at the nt and aa levels, respectively. Anti-chicken CD8alpha and CD28 monoclonal antibodies were demonstrated to specifically cross-react with turkey CD8alpha and CD28, respectively.
Powell
Fiona
F
Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.
Lawson
Michelle
M
Rothwell
Lisa
L
Kaiser
Pete
P
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D018106:CD28 Antigens / Q000235:genetics / Q000276:immunology*; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology*; D016827:CD8 Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019556:COS Cells; D002460:Cell Line; D002645:Chickens / Q000235:genetics / Q000276:immunology; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D003429:Cross Reactions / Q000276:immunology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014422:Turkeys / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018106:CD28 Antigens; D015704:CD4 Antigens; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain