Inhibition of syndecan-1 expression and function in oral cancer cells.
Syndecan-1 has been shown to be a prognostic factor in various types of tumors, suggesting its correlation with malignancy and metastasis. In the present study, we examined the expression of syndecan-1 in oral cancer cell lines and tested whether transfection of an siRNA against human syndecan-1 affected the malignant potential of these cells. Seven different human oral cancer cell lines (HSC2, HSC3, HSC4, Ca9-22, SAS, KB and BSC-OF) were used. To evaluate the mRNA expression of syndecan-1 in these cell lines, quantitative real-time RT-PCR (QRT-PCR) was carried out. In order to examine syndecan-1 function, siRNA was transfected into the cells, after which the cell growth rate and invasive ability were evaluated. As a negative control, a random sequence siRNA was used. QRT-PCR showed that syndecan-1 was expressed in Ca9-22 cells and that it was significantly higher (> 10-fold) than in the other oral cancer cell lines. Transfection of syndecan-1 siRNA was carried out on Ca9-22 cells, which increased their growth rate 1.4-fold above controls. The invasive ability of Ca9-22 cells treated with syndecan-1 siRNA was significantly higher (2-fold; n=5) than the controls. These results suggest that Ca9-22 oral cancer cells are a useful model to study syndecan-1 function and they show that syndecan-1 directly contributes to the growth and invasive ability of these cells.
Muramatsu
Takashi
T
Department of Pathology, Tokyo Dental College, Chiba 261-8502, Japan. tmuramat@tdc.ac.jp
Saitoh
Masato
M
Ro
Yasufumi
Y
Uekusa
Tomoko
T
Iwamura
Eri
E
Ohta
Kazumasa
K
Kohno
Yohko
Y
Abiko
Yoshihiro
Y
Shimono
Masaki
M
D045744:Cell Line, Tumor; D002465:Cell Movement; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D004354:Drug Screening Assays, Antitumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007797:Laminin / Q000737:chemistry; D008954:Models, Biological; D009062:Mouth Neoplasms / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011379:Prognosis; D011509:Proteoglycans / Q000737:chemistry; D034741:RNA, Small Interfering / Q000378:metabolism; D053668:Syndecan-1 / Q000096:biosynthesis*; D013997:Time Factors; D014162:Transfection
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D034741:RNA, Small Interfering; D053668:Syndecan-1; C059485:matrigel; D003094:Collagen