title: Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver.
abstract: Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1alpha was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl(4)-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1alpha, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl(4) damaged liver.
authors.affiliation: Department of Microbiology, Ewha Womans University College of Medicine, Ewha Medical Research Center, Seoul, South Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Ju
authors.forename: Kyung-Ah
authors.initials: KA
authors.lastname: Cho
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Cho
authors.forename: Yun-Jae
authors.initials: YJ
authors.lastname: Jung
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Woo
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Seoh
authors.forename: Ho-Seong
authors.initials: HS
authors.lastname: Han
authors.forename: Kyung-Ha
authors.initials: KH
authors.lastname: Ryu
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology*; D016026:Bone Marrow Transplantation; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism; D015687:Cell Hypoxia; D002465:Cell Movement / Q000502:physiology*; D054377:Chemokine CXCL12 / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019718:Receptors, CXCR4 / Q000187:drug effects / Q000378:metabolism
chemical_list: D054377:Chemokine CXCL12; D019718:Receptors, CXCR4