Evaluation of a gel-permeation high-performance liquid chromatography for determining triglyceride levels in serum major lipoproteins, compared with the ultracentrifugation/precipitation method.
OBJECTIVE
To evaluate the gel permeation high-performance liquid chromatography (GP-HPLC) method for determination of triglyceride (TG) levels in low-density lipoprotein (LDL) and high-density lipoprotein (HDL).


METHODS
The GP-HPLC and the ultracentrifugation (UC)/precipitation methods were used and compared.


RESULTS
There was no significant difference in measured levels of LDL-triglyceride between the two methods, but the HDL-triglyceride levels measured by the GP-HPLC technique were significantly higher than the UC/precipitation one (145+/-47 mg/L and 121+/-45 mg/L respectively, n=38, p<0.0001).


CONCLUSIONS
A GP-HPLC technique provides LDL-TG levels comparable to those obtained by the UC/precipitation method.
Usui
Shinichi
S
Department of Medical Technology, Okayama University Graduate School of Health Sciences, Okayama, Japan.
Mizuno
Tetsuya
T
Okazaki
Mitsuyo
M
Nakamura
Masakazu
M
Sakurabayashi
Ikunosuke
I
D011232:Chemical Precipitation; D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D015203:Reproducibility of Results; D014280:Triglycerides / Q000097:blood*; D014461:Ultracentrifugation / Q000379:methods*
C034396:HDL-triglyceride; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D014280:Triglycerides