Experimental infection with Toxocara cati in BALB/c mice, migratory behaviour and pathological changes.
Experimental inoculations of 1000 Toxocara cati larval eggs were carried out in 18 BALB/c mice. The T. cati eggs used for inoculation were collected from the faeces of naturally infected cats. Euthanasia was performed on two mice on days 1, 2, 3, 4, 5, 6, 14, 21 and 28 post-inoculation (p.i.). Tissue samples were taken for digestion and histopathology. Larvae were recovered from all infected mice and the average of all larvae recovered was 28.3% (95%; CI: 14.1-42.4). Maximum number was obtained from liver on days 1 and 2 p.i.; from the lung on day 2 p.i. and from the brain on day 28 p.i. In muscle, the recovery was high as from day 3 p.i., with the maximum obtained on day 28 p.i. Superficial foci of congestion and haemorrhage were macroscopically observed in the lungs between days 2 and 5 p.i. and in the brain between days 3 and 6 p.i. Microscopic lesions were observed in the liver between days 2 and 14 p.i., with periportal and subcapsule inflammatory infiltrates. In the lungs, haemorrhages and inflammatory infiltrates can be observed in the alveolar parenchyma, close to bronchioles and large blood vessels. In the brain, congestive areas without inflammatory reactions were seen. In muscle, the presence of inflammatory infiltrates and degenerated muscle can be observed surrounding a parasite larva. These same lesions were observed in myocardium and pericardium. The kidneys were congested with inflammatory infiltrates. The inflammatory cells present in all the tissues studied were lymphocytes, neutrophils and a few eosinophils. Formation of granulomas or signs of larva encapsulation were not observed. The migratory pattern of T. cati larvae in BALB/c mice and its tendency to become concentrated in the muscle reinforce the importance of the mouse as a paratenic host for the parasite's cycle in the environment.
Cardillo
N
N
Cátedra de Parasitología y Enfermedades Parasitarias, Universidad de Buenos Aires, Buenos Aires, Argentina. ncardillo@fvet.uba.ar
Rosa
A
A
Ribicich
M
M
López
C
C
Sommerfelt
I
I
D000704:Analysis of Variance; D000818:Animals; D001344:Autopsy / Q000662:veterinary; D001921:Brain / Q000469:parasitology / Q000473:pathology; D002371:Cat Diseases / Q000469:parasitology / Q000635:transmission; D002415:Cats; D004195:Disease Models, Animal; D005243:Feces / Q000469:parasitology; D005260:Female; D007814:Larva; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008168:Lung / Q000469:parasitology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000469:parasitology; D012376:Rodent Diseases / Q000469:parasitology* / Q000473:pathology / Q000635:transmission; D013997:Time Factors; D014119:Toxocara / Q000254:growth & development* / Q000302:isolation & purification; D014120:Toxocariasis / Q000469:parasitology* / Q000473:pathology / Q000635:transmission