An effective method for silver-staining DNA in large numbers of polyacrylamide gels.
Silver-staining of nucleic acid has been used for various biological analyses, including polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. A variety of methods have been described, but these methods are not that effective for staining more than a few PCR-SSCP gels, especially rapidly and with high sensitivity, because they include a number of time-consuming or hazardous manual steps that are often time dependent. Here we report a silver-staining method that can efficiently stain up to 14 gels at one time and with a detection limit of approximately 10 pg of DNA/mm(2), which is comparable to other methods.
Byun
S O
SO
Gene-Marker Laboratory, Agriculture and Life Sciences Division, Lincoln University, Lincoln 7647, New Zealand.
Fang
Q
Q
Zhou
H
H
Hickford
J G H
JG
D000180:Acrylic Resins / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012680:Sensitivity and Specificity; D012834:Silver / Q000032:analysis* / Q000737:chemistry; D016622:Silver Staining / Q000379:methods*
D000180:Acrylic Resins; C016680:polyacrylamide gels; D012834:Silver; D004247:DNA