title: The use of partially porous particle columns for the routine, generic analysis of biological samples for pharmacokinetic studies in drug discovery by reversed-phase ultra-high performance liquid chromatography-tandem mass spectrometry.
abstract: Recent years have seen the introduction of new high performance liquid chromatography (HPLC) instruments and columns that are capable of achieving high resolution, high speed liquid chromatographic separations at back pressures up to 1000 bar, so-called ultra-high performance liquid chromatography (UHPLC). Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) is gaining widespread use for this purpose, and for this approach to be successful a generically applicable, robust column is required. Here, data are presented showing the robustness of a partially porous 2.7 microm diameter particle material in this application and the accuracy and precision of an assay for a typical pharmaceutical in plasma. This stationary phase material is evaluated for performance and compared with other materials frequently used for similar analyses using a test mix currently used routinely in our laboratories to assess the performance of UHPLC-MS/MS systems. The partially porous material demonstrates similar resolving power to sub-2 microm materials under the ballistic gradient chromatography conditions employed and exhibits excellent resilience over the analysis of thousands of protein precipitated plasma extracts. It is suggested that this stationary phase material can be an invaluable tool in generic, high throughput assays for pharmaceutical bioanalysts.
authors.affiliation: Respiratory CEDD DMPK, GlaxoSmithKline Medicines Research Centre, Gunnels Wood Road, Stevenage, Herts SG1 2NY, UK. dave.n.mallett@gsk.com
authors.forename: David N
authors.initials: DN
authors.lastname: Mallett
authors.forename: César
authors.initials: C
authors.lastname: Ramírez-Molina
mesh_terms: D001681:Biological Assay / Q000379:methods; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D055808:Drug Discovery; D016568:Drugs, Generic / Q000032:analysis* / Q000493:pharmacokinetics*; D010316:Particle Size; D004364:Pharmaceutical Preparations / Q000097:blood; D016062:Porosity; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D016568:Drugs, Generic; D004364:Pharmaceutical Preparations