A simple and rapid fluorimetric method for simultaneous determination of protoporphyrin IX and zinc protoporphyrin IX in whole blood.
Derivative variable-angle synchronous fluorescence (DVASF) spectrometry improves the spectral resolution and selectivity of the fluorescence method. The feasibility of DVASF spectrometry for the simultaneous determination of protoporphyrin IX (PP) and zinc protoporphyrin IX (ZnPP) was investigated. PP and ZnPP were distinguished from each other simultaneously and rapidly by the DVASF method. The spectra were resolved well, and the two components were determined in a single scan, avoiding the spectral compensation factor for PP and chromatographic separation. The linear range of the calibration curve for PP was from 0.190 to 152 nmol/L and for ZnPP was from 0.383 to 230 nmol/L. The detection limits of PP and ZnPP were 0.098 nmol/L and 0.088 nmol/L, respectively. The within-run imprecision (RSD, n = 5) for PP was 4.1%, and for ZnPP was 4.2%. Mean recoveries (SD) of PP and ZnPP added to a blood sample were 86.4 (7.3)% and 72.9 (6.6)%, respectively. This method should be a potential tool in the rapid routine screening of large quantities of samples.
Zhou
Pei-Chen
PC
Department of Chemistry, Xiamen University, Xiamen, 361005, Fujian Province, China.
Huang
Wei
W
Zhang
Rong-Bin
RB
Zou
Zhe-Xiang
ZX
Luo
He-Dong
HD
Falih
Ali Abbas
AA
Li
Yao-Qun
YQ
D002138:Calibration; D005240:Feasibility Studies; D006419:Heme Oxygenase (Decyclizing) / Q000097:blood*; D006801:Humans; D017319:Photosensitizing Agents / Q000097:blood*; D011524:Protoporphyrins / Q000097:blood*; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
D017319:Photosensitizing Agents; D011524:Protoporphyrins; C017803:zinc protoporphyrin; C028025:protoporphyrin IX; D006419:Heme Oxygenase (Decyclizing)