title: Interrelationship between dendritic cell trafficking and Francisella tularensis dissemination following airway infection.
abstract: Francisella tularensis, the etiological agent of the inhalation tularemia, multiplies in a variety of cultured mammalian cells. Nevertheless, evidence for its in vivo intracellular residence is less conclusive. Dendritic cells (DC) that are adapted for engulfing bacteria and migration towards lymphatic organs could serve as potential targets for bacterial residence and trafficking. Here, we focus on the in vivo interactions of F. tularensis with DC following airway infection of mice. Lethal airway infection of mice with the live vaccine strain (LVS) results in trafficking of a CD11b(high)/CD11c(med)/autofluorescence(low) DC subset from the respiratory tract to the draining mediastinal lymph node (MdLN). Simultaneously, a rapid, massive bacterial colonization of the MdLN occurs, characterized by large bacterial foci formation. Analysis of bacteria in the MdLN revealed a major population of extracellular bacteria, which co-exists with a substantial fraction of intracellular bacteria. The intracellular bacteria are viable and reside in cells sorted for DC marker expression. Moreover, in vivo vital staining experiments indicate that most of these intracellular bacteria ( approximately 75%) reside in cells that have migrated from the airways to the MdLN after infection. The correlation between DC and bacteria accumulation in the MdLN was further demonstrated by manipulating DC migration to the MdLN through two independent pathways. Impairment of DC migration to the MdLN, either by a sphingosine-1-phosphate receptor agonist (FTY720) or by the D prostanoid receptor 1 agonist (BW245C), resulted in reduced bacterial colonization of MdLN. Moreover, BW245C treatment delayed the onset of morbidity and the time to death of the infected mice. Taken together, these results suggest that DC can serve as an inhabitation niche for F. tularensis in the early stages of infection, and that DC trafficking plays a role in pathogen dissemination. This underscores the therapeutic potential of DC migration impairing drugs in tularemia treatment.
authors.affiliation: Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona, Israel.
authors.forename: Erez
authors.initials: E
authors.lastname: Bar-Haim
authors.forename: Orit
authors.initials: O
authors.lastname: Gat
authors.forename: Gal
authors.initials: G
authors.lastname: Markel
authors.forename: Hila
authors.initials: H
authors.lastname: Cohen
authors.forename: Avigdor
authors.initials: A
authors.lastname: Shafferman
authors.forename: Baruch
authors.initials: B
authors.lastname: Velan
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology*; D005604:Francisella tularensis / Q000502:physiology*; D008198:Lymph Nodes; D051379:Mice; D050296:Microbial Viability; D012137:Respiratory System; D012141:Respiratory Tract Infections / Q000209:etiology / Q000382:microbiology; D014406:Tularemia / Q000209:etiology* / Q000382:microbiology