title: Atlas of gene expression in the developing kidney at microanatomic resolution.
abstract: Kidney development is based on differential cell-type-specific expression of a vast number of genes. While multiple critical genes and pathways have been elucidated, a genome-wide analysis of gene expression within individual cellular and anatomic structures is lacking. Accomplishing this could provide significant new insights into fundamental developmental mechanisms such as mesenchymal-epithelial transition, inductive signaling, branching morphogenesis, and segmentation. We describe here a comprehensive gene expression atlas of the developing mouse kidney based on the isolation of each major compartment by either laser capture microdissection or fluorescence-activated cell sorting, followed by microarray profiling. The resulting data agree with known expression patterns and additional in situ hybridizations. This kidney atlas allows a comprehensive analysis of the progression of gene expression states during nephrogenesis, as well as discovery of potential growth factor-receptor interactions. In addition, the results provide deeper insight into the genetic regulatory mechanisms of kidney development.
authors.affiliation: Division of Developmental Biology, Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
authors.forename: Eric W
authors.initials: EW
authors.lastname: Brunskill
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Aronow
authors.forename: Kylie
authors.initials: K
authors.lastname: Georgas
authors.forename: Bree
authors.initials: B
authors.lastname: Rumballe
authors.forename: M Todd
authors.initials: MT
authors.lastname: Valerius
authors.forename: Jeremy
authors.initials: J
authors.lastname: Aronow
authors.forename: Vivek
authors.initials: V
authors.lastname: Kaimal
authors.forename: Anil G
authors.initials: AG
authors.lastname: Jegga
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: Sean
authors.initials: S
authors.lastname: Grimmond
authors.forename: Andrew P
authors.initials: AP
authors.lastname: McMahon
authors.forename: Larry T
authors.initials: LT
authors.lastname: Patterson
authors.forename: Melissa H
authors.initials: MH
authors.lastname: Little
authors.forename: S Steven
authors.initials: SS
authors.lastname: Potter
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007668:Kidney / Q000196:embryology* / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009399:Nephrons / Q000196:embryology / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis