Suppression of matrix-related ions using cyclodextrin in MALDI mass spectrometry.
An effective technique for the suppression of matrix-related ions in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry have been developed. Using typical organic matrices such as THAP (2,4,6-trihydroxyacetophenone) and CHCA (alpha-cyano-4-hydroxycinnamic acid) in a cyclodextrin cavity, we successfully measured the mass peaks of only protonated matrix ions and significantly suppressed their intensities and fragmentation. In addition, it became possible to analyze the mass peak of the analyte molecules (substance P and adenosine) without any interference from the matrix. We believe that this technique could be a powerful tool for MALDI mass spectrometry, particularly for low-molecular-weight compounds.
Yamaguchi
Sohta
S
Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan.
Fujita
Takashi
T
Fujino
Tatsuya
T
Korenaga
Takashi
T
D000241:Adenosine / Q000032:analysis; D003373:Coumaric Acids; D003505:Cyclodextrins / Q000737:chemistry*; D007477:Ions; D008722:Methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000592:standards; D013373:Substance P / Q000032:analysis
D003373:Coumaric Acids; D003505:Cyclodextrins; D007477:Ions; C007175:alpha-cyano-4-hydroxycinnamate; D013373:Substance P; D000241:Adenosine