Determination of a hydrophilic paclitaxel derivative, 7-xylosyl-10-deacetylpaclitaxel in rat plasma by LC-MS/MS.
A LC-MS/MS method for the determination of a hydrophilic paclitaxel derivative 7-xylosyl-10-deacetylpaclitaxel in rat plasma was developed to evaluate the pharmacokinetics of 7-xylosyl-10-deacetylpaclitaxel in the rats. 7-Xylosyl-10-deacetylpaclitaxel and docetaxel (IS for 7-xylosyl-10-deacetylpaclitaxel) were extracted from rat plasma with acetic ether and analyzed on a Hypersil C(18 )column (4.6 x 150 mm i.d., particle size 5 microm) with the mobile phase of ACN/0.05% formic acid (50:50, v/v). The analytes were detected using an ESI MS/MS in the multiple reaction monitoring mode. The standard curves for 7-xylosyl-10-deacetylpaclitaxel in plasma were linear (r > 0.999) over the concentration range of 2.0-1000 ng/mL with a weighting of 1/concentration(2). The method showed a satisfactory sensitivity (2.0 ng/mL using 50 microL plasma), precision (CV <or= 10.1%), accuracy (relative error -12.4 to 12.0%), and selectivity. This method was successfully applied to the pharmacokinetic study of 7-xylosyl-10-deacetylpaclitaxel in rat plasma after intravenous administration of 7-xylosyl-10-deacetylpaclitaxel to female Wistar rats.
Jiang
Shou Gang
SG
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, People's Republic of China.
Zu
Yuan Gang
YG
Zhang
Lin
L
Fu
Yu Jie
YJ
Zhang
Yu
Y
Wang
Zhou
Z
Hua
Xin
X
Wang
Jing Tao
JT
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077143:Docetaxel; D005260:Female; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D043823:Taxoids / Q000097:blood; D013997:Time Factors
C531111:7-xylosyl-10-deacetylpaclitaxel; D043823:Taxoids; D000077143:Docetaxel; D017239:Paclitaxel