S-nitrosylation of AtSABP3 antagonizes the expression of plant immunity.
Changes in cellular redox status are a well established response across phyla following pathogen challenge. In this context, the synthesis of nitric oxide (NO) is a conspicuous feature of plants responding to attempted microbial infection and this redox-based regulator underpins the development of plant immunity. However, the associated molecular mechanism(s) have not been defined. Here we show that NO accretion during the nitrosative burst promotes increasing S-nitrosylation of the Arabidopsis thaliana salicylic acid-binding protein 3 (AtSABP3) at cysteine (Cys) 280, suppressing both binding of the immune activator, salicylic acid (SA), and the carbonic anhydrase (CA) activity of this protein. The CA function of AtSABP3 is required for the expression of resistance in the host against attempted pathogen infection. Therefore, inhibition of AtSBAP3 CA function by S-nitrosylation could contribute to a negative feedback loop that modulates the plant defense response. Thus, AtSABP3 is one of the first targets for S-nitrosylation in plants for which the biological function of this redox-based post-translational modification has been uncovered. These data provide a molecular connection between the changes in NO levels triggered by attempted pathogen infection and the expression of disease resistance.
Wang
Yi-Qin
YQ
Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
Feechan
Angela
A
Yun
Byung-Wook
BW
Shafiei
Reza
R
Hofmann
Andreas
A
Taylor
Paul
P
Xue
Peng
P
Yang
Fu-Quan
FQ
Xie
Zhen-Sheng
ZS
Pallas
Jacqueline A
JA
Chu
Cheng-Cai
CC
Loake
Gary J
GJ
D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry; D008958:Models, Molecular; D009589:Nitrogen Oxides / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology; D011485:Protein Binding; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary
D029681:Arabidopsis Proteins; D009589:Nitrogen Oxides; D002256:Carbonic Anhydrases; C539168:SABP3 protein, Arabidopsis