A polymer lab-on-a-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics.
An innovative polymer lab-on-a-chip (LOC) for reverse transcription (RT)-polymerase chain reaction (PCR) has been designed, fabricated, and characterized for point-of-care testing (POCT) clinical diagnostics. In addition, a portable analyzer that consists of a non-contact infrared (IR) based temperature control system for RT-PCR process and an optical detection system for on-chip detection, has also been developed and used to monitor the RT-PCR LOC. The newly developed LOC and analyzer have been interfaced and optimized for performing RT-PCR procedures and chemiluminescence assays in sequence. As a clinical diagnostic application, human immunodeficiency virus (HIV) for the early diagnosis of acquired immune deficiency syndrome (AIDS) has been successfully detected and analyzed using the newly developed LOC and analyzer, where the primer sets for p24 and gp120 were used as the makers for HIV. The developed polymer LOC and analyzer for RT-PCR can be used for POCT for the analysis of HIV with the on-chip RT-PCR and chemiluminescence assays in shorter than one hour with minimized cross-contamination.
Lee
Soo Hyun
SH
Microsystems and BioMEMS Laboratory, Department of Electrical and Computer Engineering, University of Cincinnati, Cincinnati, OH 45221, USA.
Kim
Sung-Woo
SW
Kang
Ji Yoon
JY
Ahn
Chong H
CH
D015415:Biomarkers / Q000032:analysis; D002478:Cells, Cultured; D019937:Diagnostic Techniques and Procedures; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046208:Microchip Analytical Procedures / Q000379:methods*; D019095:Point-of-Care Systems; D011108:Polymers / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015415:Biomarkers; D011108:Polymers