title: Fatty acids, unusual glycophospholipids and DNA analyses of thermophilic bacteria isolated from hot springs.
abstract: The composition of fatty acids in 12 strains of the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus was analyzed by gas chromatography-mass spectrometry. Major FAs found in the profiles included i-15:0, i-17:0, ai-15:0, i-16:0, 16:0, ai-17:0, together with some minor components. Branched FAs were predominant, forming more than 80% of all FAs measured. Fast atom bombardment-mass spectrometry was used for analysis of unusual glycophospholipids, i.e., acylglycosylcardiolipins from genera Geobacillus and Alicyclobacillus and 1-(hydroxy(2-(O-acylglycosyl-oxy)hexadecyloxy)phosphoryloxy) hexadecan-2-yl esters of C15-C17 acids from genera Thermus and Meiothermus. Cloning and preliminary sequence analysis of 16S rDNA showed that these isolates belong to the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus.
authors.affiliation: Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology Prague, Technicka 3, 166 28 Prague, Czech Republic. lucie.siristova@vscht.cz
authors.forename: Lucie
authors.initials: L
authors.lastname: Siristova
authors.forename: Karel
authors.initials: K
authors.lastname: Melzoch
authors.forename: Tomas
authors.initials: T
authors.lastname: Rezanka
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005227:Fatty Acids / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000302:isolation & purification*; D006358:Hot Temperature; D010743:Phospholipids / Q000302:isolation & purification*; D013045:Species Specificity; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids