Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver.
Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1alpha was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl(4)-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1alpha, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl(4) damaged liver.
Ju
Sun-Young
SY
Department of Microbiology, Ewha Womans University College of Medicine, Ewha Medical Research Center, Seoul, South Korea.
Cho
Kyung-Ah
KA
Cho
Su Jin
SJ
Jung
Yun-Jae
YJ
Woo
So-Youn
SY
Seoh
Ju-Young
JY
Han
Ho-Seong
HS
Ryu
Kyung-Ha
KH
D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology*; D016026:Bone Marrow Transplantation; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism; D015687:Cell Hypoxia; D002465:Cell Movement / Q000502:physiology*; D054377:Chemokine CXCL12 / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019718:Receptors, CXCR4 / Q000187:drug effects / Q000378:metabolism
D054377:Chemokine CXCL12; D019718:Receptors, CXCR4