Purification and characterization of a novel cholesterol-lowering protein from the seeds of Senna obtusifolia.
"Juemingzi", a source of traditional Chinese herbal medicine, has been demonstrated to play a role in decreasing serum cholesterol concentration. In this study, a novel protein, which has shown an inhibitory effect on cholesterol biosynthesis, was isolated from Senna obtusifolia L. seed by gel filtration and ion exchange chromatography. The novel protein's molecular mass was 19.7 kD and its pI was 4.80. Both SDS-PAGE and isoelectric-focusing (IEF) revealed a single Coomassie brilliant blue stained band, indicating that the novel protein was a single peptide. The N-terminal amino acid sequence of the protein was IPYISASFPLNIEFLPSE, which had no similarity with any other protein sequences in the NCBI protein database. Circular dichroism (CD) signals indicated that S. obtusifolia seed protein contained 12.5% alpha-helix, 55.6% beta-sheet, and 31.9% random coil.
Li
ChuHua
C
College of Life Sciences, South China Normal University, Guangdong Provincial Key Lab of Biotechnology of Plant Development, Guangzhou, 510631, China. lich@scnu.edu.cn
Li
Mei
M
Chang
WenRui
W
Guo
BaoJiang
B
D000595:Amino Acid Sequence; D000818:Animals; D000924:Anticholesteremic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D016466:CHO Cells; D002784:Cholesterol / Q000096:biosynthesis; D002942:Circular Dichroism; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D029846:Senna Plant / Q000737:chemistry* / Q000235:genetics
D000924:Anticholesteremic Agents; D010940:Plant Proteins; D002784:Cholesterol