Purification and characterization of recombinant lipid storage protein-2 from Drosophila melanogaster.
Lipid storage protein 2 (Lsd 2) is a conserved insect protein that belongs to the small PAT family of proteins. PAT proteins are found associated to the lipid droplets of adipocytes and play significant roles in the regulation of triacylglycerides metabolism. Here we describe the expression and purification of Lsd2, its reconstitution in lipoprotein particles, the location of putative lipid binding sites and its secondary structure. This study provides the starting point for future studies on the mechanism of function of Lsd2. The similarities and differences between Lsd1 and Lsd2, the only PAT proteins found in insects, are discussed.
Arrese
Estela L
EL
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA.
Rivera
Laticia
L
Hamada
Masakazu
M
Soulages
Jose L
JL
D017667:Adipocytes / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D002846:Chromatography, Affinity; D002942:Circular Dichroism; D003001:Cloning, Molecular; D029721:Drosophila Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005216:Fat Body / Q000378:metabolism; D008066:Lipolysis; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013329:Structure-Activity Relationship
D029721:Drosophila Proteins; C502520:LSD-2 protein, Drosophila; D011994:Recombinant Proteins