title: Free radical scavenging activity, metal chelation and antioxidant power of some of the Indian spices.
abstract: Food constituents are the major source of various phytochemicals and micronutrients. The importance of these dietary constituents has been stressed in recent years due to their antioxidant and anticarcinogenic potential. Spices used in Indian foods such as cloves (Syzygium aromaticum), licorice (Glycyrrhiza glabra), mace (aril of Myristica fragans), and greater cardamom (Amomum subulatum) were tested for their antioxidant properties in vitro. The metal chelating activity, bleomycin dependent DNA oxidation, diphenyl-p-picryl hydrazyl (DPPH) radical scavenging activity and the ferric reducing /antioxidant power (FRAP) were measured in rat liver homogenate in presence of spices. Metal chelating activity was significantly high with all the spice extracts except mace. The spices due to higher reducing potential (in presence of bleomycin-FeCl_{3}) showed increased DNA oxidation. Cloves showed the highest DPPH radical scavenging activity, followed by licorice, mace and cardamom. FRAP values for cloves were also the highest, while other spices showed comparatively lesser FRAP values. The results show that the spices tested are strong antioxidants and may have beneficial effects on human health.
authors.affiliation: School of Biochemistry, Devi Ahilya University, Indore, Madhya Pradesh, India.
authors.forename: Amit Singh
authors.initials: AS
authors.lastname: Yadav
authors.forename: Deepak
authors.initials: D
authors.lastname: Bhatnagar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D028523:Elettaria / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology; D006035:Glycyrrhiza / Q000737:chemistry; D006834:Hydrazines / Q000737:chemistry; D007194:India; D008099:Liver / Q000737:chemistry; D008670:Metals / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017365:Spices; D027842:Syzygium / Q000737:chemistry; D002721:omega-Chloroacetophenone / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002614:Chelating Agents; D016166:Free Radical Scavengers; D006834:Hydrazines; D008670:Metals; D010851:Picrates; D010936:Plant Extracts; D002721:omega-Chloroacetophenone; C004931:1,1-diphenyl-2-picrylhydrazyl