title: Chemical enhancement techniques of bloodstain patterns and DNA recovery after fire exposure.
abstract: It is common in forensic casework to encounter situations where the suspect has set a fire to cover up or destroy possible evidence. While bloodstain pattern interpretation, chemical enhancement of blood, and recovery of deoxyribonucleic acid (DNA) from bloodstains is well documented in the literature, very little information is known about the effects of heat or fire on these types of examinations. In this study, a variety of known types of bloodstain patterns were created in a four-room structure containing typical household objects and furnishings. The structure was allowed to burn to flashover and then it was extinguished by firefighters using water. Once the structure cooled over night, the interior was examined using a bright light. The bloodstains were evaluated to see if the heat or fire had caused any changes to the patterns that would inhibit interpretation. Bloodstain patterns remained visible and intact inside the structure and on furnishings unless the surface that held the blood was totally burned away. Additionally, a variety of chemical techniques were utilized to better visualize the patterns and determine the possible presence of blood after the fire. The soot from the fire formed a physical barrier that initially interfered with chemical enhancement of blood. However, when the soot was removed using water or alcohol, the chemicals used, fluorescein, luminol, Bluestar, and Hemastix, performed adequately in most of the tests. Prior to DNA testing, the combined phenolphthalein/tetramethyl benzidine presumptive test for the presence of blood was conducted in the laboratory on samples recovered from the structure in an effort to assess the effectiveness of using this type of testing as a screening tool. Test results demonstrated that reliance on obtaining a positive presumptive result for blood before proceeding with DNA testing could result in the failure to obtain useful typing results. Finally, two DNA recovery methods (swabbing the stain plus cutting or scraping the stain) were attempted to evaluate their performance in recovering samples in an arson investigation. Recovery of DNA was more successful in some instances with the swabbing method, and in other instances with the cutting/scraping method. Therefore, it is recommended that both methods be used. For the most part, the recovered DNA seemed to be unaffected by the heat, until the temperature was 800 degrees C or greater. At this temperature, no DNA profiles were obtained.
authors.affiliation: Montgomery County Crime Laboratory, 2350 Research Blvd., Rockville, MD, USA.
authors.forename: Karolyn L
authors.initials: KL
authors.lastname: Tontarski
authors.forename: Kyle A
authors.initials: KA
authors.lastname: Hoskins
authors.forename: Tani G
authors.initials: TG
authors.lastname: Watkins
authors.forename: Leanora
authors.initials: L
authors.lastname: Brun-Conti
authors.forename: Amy L
authors.initials: AL
authors.lastname: Michaud
mesh_terms: D001560:Benzidines; D001801:Blood Stains; D002863:Chromogenic Compounds; D004247:DNA / Q000302:isolation & purification*; D005390:Fires; D019793:Fluorescein; D005456:Fluorescent Dyes; D005554:Forensic Medicine / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D007202:Indicators and Reagents; D049408:Luminescent Agents; D008165:Luminol; D020113:Phenolphthalein; D011934:Reagent Strips; D013048:Specimen Handling / Q000379:methods*
chemical_list: D001560:Benzidines; D002863:Chromogenic Compounds; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D049408:Luminescent Agents; D011934:Reagent Strips; C021758:3,3',5,5'-tetramethylbenzidine; D008165:Luminol; D020113:Phenolphthalein; D004247:DNA; D019793:Fluorescein