Porcine platelet lysate as a supplement for animal cell culture.
A novel supplementation of cell growth media based on a porcine platelet lysate was developed for culture of animal-derived cells. The platelet lysate was produced from porcine blood and contained lysate of platelets and plasma components. It showed satisfactory microbiological integrity and it carried only low amount of endotoxins (<10 EU/mL). The porcine platelet lysate supported well proliferation of Vero (African green monkey transformed kidney epithelial cells), Chinese hamster ovary (CHO) and hybridoma cells comparable to fetal bovine serum (FBS). Platelet lysate shows promise as a viable choice over FBS as it can be produced in large quantities, high lot-to-lot consistency and with an attractive price structure. Furthermore it is a strong alternative to FBS for ethical reasons. It is expected that it can be used as a general supplementation for most animal cells for research studies on the proliferation of cells and their expression of products.
Aldén
Anna
A
School of Pure and Applied Natural Sciences, University of Kalmar, SE-391 82, Kalmar, Sweden.
Gonzalez
Lorena
L
Persson
Anna
A
Christensson
Kerstin
K
Holmqvist
Olov
O
Ohlson
Sten
S