title: Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers.
abstract: The asymmetric distribution of charged molecules between the leaflets of solid-substrate-supported phospholipid bilayers is studied using imaging ellipsometry, fluorescence microscopy, and numerical solutions of the Poisson-Boltzmann equation. Experiments are facilitated by the use of patterned substrates that allow for side-by-side comparison of lipid monolayers and supported bilayers. On silica surfaces, negatively charged lipid components are shown to be enriched in the outer leaflet of a supported bilayer system at modest salt concentrations. The approaches developed provide a general means for determining asymmetries of charged components in supported lipid bilayers.
authors.affiliation: Center for Integrated Nanotechnologies, Materials Physics and Applications Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Shreve
authors.forename: Michael C
authors.initials: MC
authors.lastname: Howland
authors.forename: Annapoorna R
authors.initials: AR
authors.lastname: Sapuri-Butti
authors.forename: Toby W
authors.initials: TW
authors.lastname: Allen
authors.forename: Atul N
authors.initials: AN
authors.lastname: Parikh
mesh_terms: D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D011510:Proteolipids / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
chemical_list: D008051:Lipid Bilayers; D008567:Membranes, Artificial; D011510:Proteolipids; D012822:Silicon Dioxide