Identification of novel synthetic peptide showing angiogenic activity in human endothelial cells.
A novel synthetic hexapeptide (SFKLRY-NH(2)) that displays angiogenic activity has been identified by positional scanning of a synthetic peptide combinatorial library (PS-SPCL). The peptide induced proliferation, migration, and capillary-like tube formation in primary cultured HUVECs, and augmented vessel sprouting ex vivo while attenuated by the treatment with pertussis toxin (PTX) or U73122 (PLC-inhibitor) suggesting the influence of PTX-sensitive G-proteins and PLC. In addition, SFKLRY-NH(2) up-regulated the expression of VEGF-A in HUVECs and the neutralizing antibody against VEGF suppressed SFKLRY-NH(2)-induced tube formation activity. Taken together, these results suggest that SFKLRY-NH(2) may induce blood vessel formation by PTX-sensitive G protein-coupled receptor-PLC-Ca(2+) signaling cascade leading into VEGF-A expression in HUVECs.
Lee
Chang Hee
CH
SIGMOL Institute of Molecular Medicine, SIGMOL Inc., 790-834 Pohang, South Korea.
Lee
Mi-Sook
MS
Kim
Sun-Jin
SJ
Je
Young-Tae
YT
Ryu
Sung Ho
SH
Lee
Taehoon
T
D043925:Angiogenesis Inducing Agents / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D009389:Neovascularization, Pathologic; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D043925:Angiogenesis Inducing Agents; D009842:Oligopeptides; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C540365:seryl-phenylalanyl-lysyl-leucyl-arginyl-tyrosinamide; D002118:Calcium