Role of single-strand DNA 3'-5' exonuclease ExoI and nuclease SbcCD in stationary-phase mutation in Escherichia coli K-12.
In RecBCD(+) cells, a mutated single-strand DNA 3'-5' exonuclease ExoI (SbcB15) induced an increase in stationary-phase mutation. In sbcB15 cells, as in wild-type cells, these mutations partially required RecA, RecB, RecF, and expression of the LexA regulon. The absence of nuclease SbcCD in sbcB15 cells decreased stationary-phase mutation and induced an increase in the number of cell filaments. The absence of ExoI (Deltaxon) in wild-type or sbcC cells did not change significantly the stationary-phase mutation. Differences between the sbcB15 and DeltaxonA cells suggest a correlation between level of SOS induction and the generation of stationary-phase mutations.
Ramírez-Santos
Jesús
J
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, P.O. Box 70-228, 04510 Mexico, D.F., Mexico.
García-Mata
Verónica
V
Poggio
Sebastian
S
Camarena
Laura
L
Gómez-Eichelmann
M Carmen
MC
D003851:Deoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D005092:Exonucleases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation
D029968:Escherichia coli Proteins; C489229:SbcC protein, E coli; D003851:Deoxyribonucleases; D005090:Exodeoxyribonucleases; D005092:Exonucleases; C081229:sbcD protein, E coli; C028450:exodeoxyribonuclease I