Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor.
A process was developed for producing human menin from transformed Drosophila Schneider 2 cells. Protein expression was achieved after inducing the metallothionein promoter by adding copper sulfate to cells growing in suspension in a stirred-tank reactor. Experiments in shake flasks showed that the production of menin was improved when the induction was conducted late in the exponential phase of cell growth at a concentration of 1-2 x 10(7) cells ml(-1), with a copper concentration of 0.2 mM for no more than 24 h. This observation was confirmed by experiments in bench-scale fermentors. Subsequently, a pilot-scale fermentation yielded 1 mg l(-1) culture of purified menin.
Valle
M A
MA
Biotechnology Unit, NIDDK, NIH, Bethesda, MD, 20892, U.S.A..
Kester
M B
MB
Burns
A L
AL
Marx
S J
SJ
Spiegel
A M
AM
Shiloach
J
J