Label-free imaging of Drosophila larva by multiphoton autofluorescence and second harmonic generation microscopy.
The fruit fly Drosophila melanogaster is one of the most valuable organisms in studying genetics and developmental biology. To gain insight into Drosophila development, we successfully acquired label-free, in vivo images of both developing muscles and internal organs in a stage 2 larva using the minimally invasive imaging modality of multiphoton autofluorescence (MAF) and second harmonic generation (SHG) microscopy. We found that although MAF is useful in identifying structures such as the digestive system, trachea, and intestinal track, it is the SHG signal that allowed the investigation of the muscular architecture within the developing larva. Our results suggest that multiphoton microscopy is a powerful in vivo, label-free imaging technique to examine Drosophila physiology and may be used for developmental studies.
Lin
Chiao-Ying
CY
National Taiwan University, Department of Electrical Engineering, Taipei, Taiwan.
Hovhannisyan
Vladimir
V
Wu
June-Tai
JT
Lin
Chii-Wann
CW
Chen
Jyh-Horng
JH
Lin
Sung-Jan
SJ
Dong
Chen-Yuan
CY
D000818:Animals; D004331:Drosophila melanogaster / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods*; D007814:Larva; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013194:Staining and Labeling