Novel pH indicator dyes for array preparation via NHS ester activation or solid-phase organic synthesis.
We present two ways of fast and easy immobilisation of a naphthalimide chromophore with a pH-sensor function. The immobilised dyes exhibit absorbance and emission in the visible spectral range, large Stokes' shift, fluorescence properties that are comparable to their water-soluble form, and full reversibility in pH response.
Trupp
Sabine
S
Friedrich-Schiller University, Institute of Physical Chemistry, Lessingstrasse 10, 07743, Jena, Germany. sabine.trupp@uni-jena.de
Hoffmann
Patrick
P
Henkel
Thomas
T
Mohr
Gerhard J
GJ
D004952:Esters / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D046228:Microarray Analysis; D053644:Naphthalimides / Q000737:chemistry*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
D004952:Esters; D005456:Fluorescent Dyes; D053644:Naphthalimides; D014867:Water