title: Developmental levels of phospholipase D isozymes in the brain of developing rats.
abstract: The developmental levels of phospholipase D (PLD) isozymes was examined in the cerebrum and hindbrain of the developing rat to better understand the involvement of PLD in brain development. Western blot analysis of PLD in the cerebrum showed that PLD1, a major PLD isoform in the brain, was detected weakly in the cerebrum at day 17 embryonic stage and its levels gradually increased until postnatal day 35 and remained unaltered thereafter. In the hindbrain, comprising the cerebellum and pons, the peak level of PLD1 was detected at 21 days postnatally and declined progressively thereafter. The level of PLD2 in both the cerebrum and hindbrain was minimal compared to that of PLD1. Based on immunohistochemistry, PLD was detected in some neurons and glial cells in the cerebrum. In the hindbrain, PLD was found in some Purkinje cells and some cells of the molecular layer, as well as glial cells, consistent with the results obtained from Western blot analysis. These findings suggest that PLD may differentially play a role in the course of early development of the brain, with special reference to the cerebrum and hindbrain, in rats.
authors.affiliation: Department of Veterinary Anatomy, Applied Radiological Science Research Institute, Cheju National University, Jeju, South Korea.
authors.forename: Mia
authors.initials: M
authors.lastname: Kim
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.forename: Heechul
authors.initials: H
authors.lastname: Kim
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Shin
authors.forename: Do Sik
authors.initials: do S
authors.lastname: Min
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D054022:Cerebrum / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D010739:Phospholipase D / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007527:Isoenzymes; C105606:phospholipase D2; D010739:Phospholipase D; C106801:phospholipase D1