title: Proteomic map of Trypanosoma cruzi CL Brener: the reference strain of the genome project.
abstract: In this work two-dimensional gel electrophoresis combined with mass spectrometry was carried out in order to start the construction of a map of soluble proteins from epimastigote form of Trypanosoma cruzi CL Brener. This strain is a hybrid organism derived from two genotypes, T. cruzi I and T. cruzi II and was chosen for genome sequencing. The two-dimensional gel electrophoresis showed that most of proteins focused at 4-7 pH range. The identification demonstrated that several proteins were in multiple isoforms, such as tubulin and heat shock proteins. Potential targets for development of chemotherapeutic agents like arginine kinase, an enzyme absent from mammalian tissues that is involved in the energy supply of the parasite, were also detected.
authors.affiliation: Laboratory of Molecular Epidemiology of Infectious Diseases, Oswaldo Cruz Institute-IOC, Oswaldo Cruz Foundation-FIOCRUZ. Av. Brasil, 4365-Manguinhos, Rio de Janeiro, RJ 21045-900, Brazi. csodre@fiocruz.br
authors.forename: Cátia Lacerda
authors.initials: CL
authors.lastname: Sodré
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Chapeaurouge
authors.forename: Dário Eluan
authors.initials: DE
authors.lastname: Kalume
authors.forename: Leila
authors.initials: L
authors.lastname: de Mendonça Lima
authors.forename: Jonas
authors.initials: J
authors.lastname: Perales
authors.forename: Octavio
authors.initials: O
authors.lastname: Fernandes
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D018503:Genome, Protozoan; D008969:Molecular Sequence Data; D040901:Proteomics; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D015800:Protozoan Proteins