[Effect of nebulized TFG on Th1/Th2 imbalance in mouse model with asthma].
OBJECTIVE
To investigate the effect of nebulized total ginkgo flavone glycosides (TFG) on Th1/Th2 imbalance in mice with athma.


METHODS
Forty-eight BALB/C mice were randomly divided into four groups: group A (control group, n=12); group B (asthmatic model group, n=12); group C (TFG nebulized treated group, n=12); group D (dexamethasone intraperitoneal treated group, n=12). The asthmatic model was established by sensitivity and local activation with Ovalbumin(OVA) and aluminum hydroxide Al(OH)3. TFG (50 g x L(-1), per aerosol per six mice, 30 minutes) was nebulized 20 days after modeling, while dexamethasone (1 g x L(-1)) was intraperitoneal once daily for 10 days. Perfusate of bronchoalveolar lavage fluid(BALF) was collected on day 32. The level of IL-4, IFN-gamma in BALF, and the level of total IgE in serum was determined. The airway inflammation pathology change and the expression of GATA-3 protein in lungs was detected by immunohistochemical staining.


RESULTS
Compared with model group, the decreased content of IL-4(49.30 +/- 7.95) ng x L(-1) and increased level of IFN-gamma (49.08 x 5.46) ng x L(-1). were found in BALF, and the level of total IgE (9.47 +/- 1.52) microg x L(-1) in serum also decreased in TFG treated group. In model group, smooth muscle hypertrophing, mucous hyperemia, mucous layer thickening, and inflammatory cell in filtration were observed. Phlegmasia was appeared in the bronchi, which was filled with lots of mucus. In contrast, the inflammatory reaction in TFG and Dexamethasone treated group was less obvious. Expression of GATA-3 was markly increased in model group with decreased expression in TFG treated group.


CONCLUSIONS
Nebulized TFG showed a therapeutic effect for asthmatic mice, the mechanism may be explained by blockingnnnn GATA-3 expression and regulating the disorder Th1/Th2 imbalance.
Chen
Li-li
LL
Medical and Pharmaceutical Institute of Yangzhou University, Yangzhou 225001, China.
Weng
Xiao-jing
XJ
Li
Xin
X
Zhang
Hong-quan
HQ
D000536:Aluminum Hydroxide / Q000494:pharmacology; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D003907:Dexamethasone / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use*; D005260:Female; D047309:Flavones; D005419:Flavonoids / Q000737:chemistry*; D050990:GATA3 Transcription Factor / Q000378:metabolism; D020441:Ginkgo biloba / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry* / Q000627:therapeutic use*; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000494:pharmacology; D011897:Random Allocation; D018417:Th1 Cells / Q000187:drug effects* / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects* / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D047309:Flavones; D005419:Flavonoids; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D006027:Glycosides; D015847:Interleukin-4; D007073:Immunoglobulin E; D000536:Aluminum Hydroxide; D003907:Dexamethasone; D007371:Interferon-gamma; D010047:Ovalbumin; C043562:flavone