Effect of dentinal tubule occlusion by dentifrice containing nano-carbonate apatite.
This study evaluated the effects of the short-term use of a dentifrice containing nano-sized carbonate apatite (n-CAP) on the occlusion of the dentinal tubules using a scanning electron microscope (SEM) and an image analyser in vitro. One hundred human dentine specimens were wet ground with a silicone carbide papers and etched with 6% citric acid for 1 min to allow complete opening of the dentinal tubule. Specimens showing complete opening tubules were used as the baseline. The specimens were divided randomly into five groups: G1: 0% n-CAP, G2: 5% n-CAP, G3: 10% n-CAP, G4: 20% n-CAP and G5: 10%strontium chloride (SrCl(2)). Five specimens from each group were brushed by applying 50, 100, 250 and 500 strokes, respectively. All the specimens were evaluated by a SEM (x3000), and the degree of occlusion of the dentinal tubules was quantified using an image analyser. The results were analysed by one-way anova and a Tukey's test using the spss 12.0 statistical package program. The dentifrice containing 20% n-CAP for 50 strokes, which indicated 2-day use, showed the highest tubular occlusion than the other groups (P < 0.05). Moreover, this group showed 79.5% and 77.4% less open tubular area than the baseline and 0% n-CAP group, respectively. The groups containing various concentrations of n-CAP showed significant differences in the SrCl(2) group after tooth-brushing for 500 strokes, which indicated 17-day use. According to this examination of the short-term use of desensitizing dentifrices in vitro, the dentifrice containing 20% n-CAP was the most effective in occluding the dentinal tubules.
Lee
S Y
SY
Department of Preventive Dentistry and Public Oral Health and Research Center for Orofacial Hard Tissue Regeneration, BK 21 Project, College of Dentistry, Yonsei University, Seoul, Korea.
Kwon
H K
HK
Kim
B I
BI
D000134:Acid Etching, Dental; D001031:Apatites / Q000627:therapeutic use*; D003802:Dentifrices / Q000737:chemistry*; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D003806:Dentin Permeability / Q000187:drug effects*; D003807:Dentin Sensitivity / Q000188:drug therapy*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D014100:Toothpastes / Q000627:therapeutic use*
D001031:Apatites; D003802:Dentifrices; D014100:Toothpastes; C030782:carboapatite