Lipid peroxides induce early onset of catagen phase in murine hair cycles.
The precise mechanisms of alopecia, a pathophysiological disorder with negative psychological implications, are unknown. Androgen and hereditary predisposition are major causes, but the condition is also affected by stress, an irregular diet and high levels of sebum secretion. We focused on oxidative stress and analyzed the effect of the lipid peroxides on hair follicles. Our first observation was that the topical application of linolein hydroperoxides, one of the lipid peroxides, lead to the early onset of the catagen phase in murine hair cycles. Furthermore, by using TUNEL staining we found that lipid peroxides induced apoptosis of hair follicle cells. They also induced apoptosis in human epidermal keratinocytes by up-regulating apoptosis-related genes. These results indicated that lipid peroxides, which can cause free radicals, induce the apoptosis of hair follicle cells, and this is followed by early onset of the catagen phase. These observations may provide insight into the mechanisms underlying the development of alopecia in humans.
Naito
Atsushi
A
Biological Science Research Laboratories, Research and Development Headquarters, LION Corporation, 100 Tajima, Kanagawa 256-0811, Japan. a-naito@lion.co.jp
Midorikawa
Tatsuyuki
T
Yoshino
Teruhiko
T
Ohdera
Motoyasu
M
D000505:Alopecia; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D006197:Hair / Q000254:growth & development; D018859:Hair Follicle / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D020287:In Situ Nick-End Labeling; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects* / Q000473:pathology; D008054:Lipid Peroxides / Q000378:metabolism* / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D008054:Lipid Peroxides; D017382:Reactive Oxygen Species