Co-chaperonin GroES as a modulator of proteasomal activity.
The proteasome has a crucial part in the degradation of normal, damaged, mutant or misfolded proteins within both the ubiquitin ATP-dependent and the ubiquitin ATP-independent pathways. Proteasome-mediated proteolysis is modulated by diverse factors, and in this regard, chaperonins have been attracting great interest. The investigation on the role of a co-chaperonin, namely GroES, in the modulation of proteasomal activity was the focus of this work. Our study reports on an analytical approach based on combined fluorimetric, chromatographic (applied to the enzymatic activity evaluation), surface plasmon resonance techniques and molecular modelling, addressed to the assessment and characterization of the interaction. Globally, we described a high affinity interaction between GroES and two different 20 S (immuno- and constitutive) proteasomes, uncovering new scenarios on their possible physio-pathological role, specifically on the ability of proteasomes to interact both with unfolding and folding- assisting macromolecules.
Cuccioloni
Massimiliano
M
Department of Molecular, Cellular and Animal Biology, University of Camerino, Via Gentile III da Varano, 62032 Camerino, Italy. massimiliano.cuccioloni@unicam.it
Montecchia
Francesca
F
Amici
Manila
M
Mozzicafreddo
Matteo
M
Eleuteri
Anna Maria
AM
Angeletti
Mauro
M
D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D018835:Chaperonin 10 / Q000737:chemistry / Q000378:metabolism*; D018834:Chaperonin 60 / Q000737:chemistry / Q000378:metabolism; D018833:Chaperonins / Q000737:chemistry / Q000378:metabolism*; D003198:Computer Simulation; D005470:Fluorometry; D006801:Humans; D008958:Models, Molecular; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000378:metabolism; D020349:Surface Plasmon Resonance; D013950:Thymus Gland / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism
D018835:Chaperonin 10; D018834:Chaperonin 60; D011994:Recombinant Proteins; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex; D018833:Chaperonins