A rapid flow cytometry assay for the assessment of calcium mobilization in human neutrophils in a small volume of lysed whole-blood.
Flow cytometry-based methods have been developed to measure most neutrophil responses. The assessment of the mobilization of calcium, however, is routinely performed on neutrophils isolated from whole blood. This report describes a flow cytometry-based assay to measure the mobilization of calcium in neutrophils directly in whole blood. This method requires minimal sample manipulation, small volumes of blood and is performed in a short period of time. Both clinical and research laboratories will be able to assess neutrophil function and the quality of granulocyte preparations using a more time and cost effective calcium mobilization test.
Desmeules
Philippe
P
Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du CHUQ-CHUL, Département d'Anatomie-Physiologie, Faculté de Médecine, Université Laval, Quebec, Canada, G1V 4G2.
Dufour
Maurice
M
Fernandes
Maria J G
MJ
D001773:Blood Cells / Q000378:metabolism*; D002118:Calcium / Q000032:analysis* / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013997:Time Factors; D015054:Zymosan
D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D002118:Calcium