title: MDV-1 VP22 conjugated VP2 enhancing immune response against infectious bursal disease virus by DNA vaccination in mice.
abstract: VP22 of Marek's disease virus serotype 1 (MDV-1) could function in protein transduction. In this study, an infectious bursal disease virus VP2 gene was fused to the carboxyl termini of VP22. It showed that the fusion protein did not spread into the bystander cells from the cells transfected with pVP22-VP2, as the VP22 alone could. The VP22 proteins were found to be translocated into all the nuclei in the neighboring COS-1 cells, as analyzed by a fluorescence assay. Although mice were immunized with the recombinant DNAs mixed with polyethylenimine (PEI) at a dose of 1:2, it failed to enhance the antibody response against IBDV VP2, as measured by the indirect ELISA assay, yet the cell mediated immune response was significantly increased. The ratio of CD8(+)/CD4(+) T cells was significantly increased in the immunized group with the fusion genes, compared with the group immunized with VP2 (P<0.05). Our results demonstrated that VP22 indeed enhances the cell-mediated response in the fused VP2 in a mice model system, possibly due to the fact that the IBDV VP2 could be carried into the surrounding cells at a limited level under pressure from MDV VP22.
authors.affiliation: Key lab of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Chen
authors.forename: Chenfei
authors.initials: C
authors.lastname: Zhang
authors.forename: Cuiping
authors.initials: C
authors.lastname: Song
authors.forename: Xufang
authors.initials: X
authors.lastname: Deng
authors.forename: Aijian
authors.initials: A
authors.lastname: Qin
mesh_terms: D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000276:immunology* / Q000517:prevention & control*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000276:immunology*; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology*; D022501:Marek Disease Vaccines / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018074:Vaccines, Conjugate / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D004279:DNA, Viral; D022501:Marek Disease Vaccines; C450446:UL49 protein, Marek's disease virus type 1; C082513:VP2 protein, infectious bursal disease virus; D018074:Vaccines, Conjugate; D019444:Vaccines, DNA; D014764:Viral Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines