Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana.
In Arabidopsis, catalase (CAT) genes encode a small family of proteins including CAT1, CAT2 and CAT3, which catalyze the decomposition of hydrogen peroxide (H2O2) and play an important role in controlling homeostasis of reactive oxygen species (ROS). Here, we analyze the expression profiles and activities of three catalases under different treatments including drought, cold, oxidative stresses, abscisic acid and salicylic acid in Arabidopsis. Our results reveal that CAT1 is an important player in the removal of H2O2 generated under various environmental stresses. CAT2 and CAT3 are major H2O2 scavengers that contribute to ROS homeostasis in light or darkness, respectively. In addition, CAT2 is activated by cold and drought stresses and CAT3 is mainly enhanced by abscisic acid and oxidative treatments as well as at the senescence stage. These results, together with previous data, suggest that the network of transcriptional control explains how CATs and other scavenger enzymes such as peroxidase and superoxide dismutase may be coordinately regulated during development, but differentially expressed in response to different stresses for controlling ROS homeostasis.
Du
Yan-Yan
YY
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Wang
Peng-Cheng
PC
Chen
Jia
J
Song
Chun-Peng
CP
D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000502:physiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D020156:Salicylic Acid / Q000494:pharmacology
D029681:Arabidopsis Proteins; D007527:Isoenzymes; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D002374:Catalase; D020156:Salicylic Acid