Mechanism of the inhibitory action of chestnut astringent skin extract on carbohydrate absorption.
Chestnut astringent skin (CAS) extract inhibited pancreatic alpha-amylase and intestinal alpha-glucosidase in a concentration-dependent manner with the 50% inhibition concentration (IC50) for amylase, maltase and sucrase being 7.5, 650 and 390 microg/mL, respectively. We have investigated the effect of CAS extract on carbohydrate absorption in normal rats. Oral administration of CAS extract to rats fed cornstarch (2 g/kg body weight) significantly suppressed the increase of blood glucose levels and the area under the curve (AUC). Administration of CAS extract to rats fed maltose or sucrose delayed the increase of blood glucose level and slightly suppressed AUC, but not significantly. Administration of CAS extract to rats fed glucose did not affect the increase in blood glucose level or AUC. Similar results were observed with type-2 diabetic model rats (GK/jcl). To test the effect of CAS extract on diabetes, type 2 diabetic model mice (db/db mice) were fed a standard laboratory diet containing 1 or 2% CAS extract. CAS extract prevented increases in body weight and fasting blood glucose concentration. These data suggest that CAS extract has an anti-diabetic function in type 2 diabetic mice that mainly functions through inhibition of alpha-amylase.
Tsujita
Takahiro
T
Bioscience, Integrated Center for Sciences, Ehime University, Ehime, Japan. tsujita@m.ehime-u.ac.jp
Takaku
Takeshi
T
D000818:Animals; D019540:Area Under Curve; D001252:Astringents / Q000494:pharmacology*; D001786:Blood Glucose / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D002241:Carbohydrates / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D029962:Fagaceae / Q000737:chemistry; D065089:Glycoside Hydrolase Inhibitors; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D009754:Nuts; D019441:Plant Epidermis; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D013393:Sucrase / Q000037:antagonists & inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D001252:Astringents; D001786:Blood Glucose; D002241:Carbohydrates; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000516:alpha-Amylases; D013393:Sucrase