Effect of dietary n-6/n-3 ratio on liver n-6/n-3 ratio and peroxisomal beta-oxidation activity in rats.
The optimal dietary n-6/n-3 ratio has not been fully elucidated. To investigate the influence of the dietary n-6/n-3 ratio on this ratio in the body and on liver beta-oxidation peroxisomal activity, rats were fed diets containing fat at an n-6/n-3 ratio of 1 to 16 for 4 weeks. To investigate whether elevation of the liver peroxisomal beta-oxidation activity increases the n-6/n-3 ratio in the body, rats were fed a diet containing a peroxisome-activating agent, bezafibrate, for 2 weeks, and its influence on the liver n-6/n-3 ratio was examined. The slope of the regression line between the dietary and liver total lipid n-6/n-3 ratios was significantly smaller when the dietary n-6/n-3 ratio was 4 or greater than when it was smaller than 4. Peroxisomal beta-oxidation and acyl CoA oxidase activities were significantly lower in rats fed a diet with an n-6/n-3 ratio of 16 than in those fed a diet with a ratio of 1. The peroxisomal beta-oxidation activity in the bezafibrate-supplemented group was significantly higher than that in the control group. The serum and liver total lipid n-6/n-3 ratios were significantly higher in the 0.015% bezafibrate-supplemented group than in the control group. These findings suggest that the liver n-6/n-3 ratio might be controlled via peroxisomal beta-oxidation in rats.
Takeuchi
Hiroyuki
H
Central Research Laboratory, The Nisshin Oillio Group, Yokosuka, Kanagawa, Japan. h-takeuchi@nisshin-oillio.com
Kojima
Keiichi
K
Sekine
Seiji
S
Murano
Yoshihiro
Y
Aoyama
Toshiaki
T
D000818:Animals; D001629:Bezafibrate / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D004041:Dietary Fats / Q000097:blood / Q000494:pharmacology*; D015525:Fatty Acids, Omega-3 / Q000097:blood / Q000494:pharmacology*; D043371:Fatty Acids, Omega-6 / Q000097:blood / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D020675:Peroxisomes / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011913:Rats, Inbred BB
D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D000960:Hypolipidemic Agents; D001629:Bezafibrate