title: Quantitative overview of N2 fixation in Nostoc punctiforme ATCC 29133 through cellular enrichments and iTRAQ shotgun proteomics.
abstract: Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N 2. Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both the filament level and the heterocyst level are presented using isobaric tagging technology (iTRAQ), with 721 proteins at the 95% confidence interval quantified across both studies. Observations from both experiments yielded findings confirmatory of both transcriptional studies, and published Nostoc sp. PCC 7120 iTRAQ data. N. punctiforme exhibits similar metabolic trends, though changes in a number of metabolic pathways are less pronounced than in Nostoc sp. PCC 7120. Results also suggest a number of proteins that may benefit from future investigations. These include ATP dependent Zn-proteases, N-reserve degraders and also redox balance proteins. Complementary proteomic data sets from both organisms present key precursor knowledge that is important for future cyanobacterial biohydrogen research.
authors.affiliation: Biological & Environmental Systems Group, The University of Sheffield, Sheffield, S1 3JD, UK.
authors.forename: Saw Yen
authors.initials: SY
authors.lastname: Ow
authors.forename: Josselin
authors.initials: J
authors.lastname: Noirel
authors.forename: Tanal
authors.initials: T
authors.lastname: Cardona
authors.forename: Arnaud
authors.initials: A
authors.lastname: Taton
authors.forename: Peter
authors.initials: P
authors.lastname: Lindblad
authors.forename: Karin
authors.initials: K
authors.lastname: Stensjö
authors.forename: Philip C
authors.initials: PC
authors.lastname: Wright
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D000458:Cyanobacteria / Q000378:metabolism; D006859:Hydrogen / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D009584:Nitrogen / Q000737:chemistry; D009586:Nitrogen Fixation; D046937:Nostoc / Q000378:metabolism*; D010084:Oxidation-Reduction; D010447:Peptide Hydrolases / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods; D015032:Zinc / Q000737:chemistry
chemical_list: D006859:Hydrogen; D000255:Adenosine Triphosphate; D010447:Peptide Hydrolases; D015032:Zinc; D009584:Nitrogen