Oxidative stress profile in the post-operative patients with biliary atresia.
OBJECTIVE
Many post-operative patients with biliary atresia (BA) suffer from liver dysfunction, such as chronic inflammation even without jaundice after a Kasai's hepatic portoenterostomy.


METHODS
The presence and degree of oxidative stress were evaluated in the post-operative patients with BA. Twelve outpatients who underwent a Kasai's hepatic portoenterostomy were evaluated. The active oxygen products, the rate of bioantioxidant, the markers of oxidative stress, and the degree of hepatic oxidative stress were examined by immunohistochemical staining of biopsied specimens.


RESULTS
All of the oxidative stress markers in the post-operative patients with BA increased in comparison to those in the controls. Moreover, 8-OHdG immunohistochemical staining was positive in 84+/-4.8% in hepatic cells in the portal area in the post-operative patients with BA.


CONCLUSIONS
The post-operative patients with BA were under increased oxidative stress, even if their liver dysfunction was mild without jaundice. Antioxidant therapy might be necessary to decrease of oxidative stress in the post-operative patients with BA.
Asakawa
Takahiro
T
Department of Pediatric Surgery, Kurume University School of Medicine, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan. monmon@ka3.so-net.ne.jp
Tanaka
Yoshiaki
Y
Asagiri
Kimio
K
Kobayashi
Hidefumi
H
Tanikawa
Ken
K
Yagi
Minoru
M
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000410:Alanine Transaminase / Q000378:metabolism; D001656:Biliary Atresia / Q000378:metabolism* / Q000601:surgery*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D024141:Collagen Type IV / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D015237:Dinoprost / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D011171:Portoenterostomy, Hepatic; D011184:Postoperative Period; D013482:Superoxide Dismutase / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D015415:Biomarkers; D024141:Collagen Type IV; C075750:8-epi-prostaglandin F2alpha; D007334:Insulin-Like Growth Factor I; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D013482:Superoxide Dismutase; D005723:gamma-Glutamyltransferase; D000410:Alanine Transaminase; D003849:Deoxyguanosine