Survivin expression in esophageal cancer: correlation with p53 mutations and promoter polymorphism.
Survivin is an inhibitor of apoptosis protein, which is selectively up-regulated in various cancers including esophageal cancer. The underlying mechanism of survivin overexpression in cancers is still unclear. We investigated resected tumor specimens from 100 esophageal cancer patients. Reverse transcription polymerase chain reaction was performed to evaluate survivin gene expression. Polymerase chain reaction-single strand conformation polymorphism was performed to investigate mutations of p53. We found that the survivin expression in tumors with mutant p53 is higher than that in tumors with wild type p53. Furthermore, the distribution of three polymorphisms in survivin promoter region in esophageal cancer patients was studied. The result indicated that the survivin expression was caused by a C allele in the survivin promoter polymorphism -625G/C in some degree. The methylation profile of survivin exon1 was also evaluated using bisulfite sequencing PCR. Our result indicated that survivin mRNA overexpression in cancer was not caused by its dysmethylation status. Therefore, our results suggested that the survivin expression depended on the p53 status and the C allele in the survivin promoter polymorphism -625G/C might increase the possibility of the survivin overexpression in esophageal cancer patients.
Yang
Xiaoya
X
Department of Medical Genetics, Basic Medicine, Southwest Hospital, Third Military Medical University, Chongqing, China.
Xiong
Gang
G
Chen
Xuedan
X
Xu
Xueqing
X
Wang
Kai
K
Fu
Yong
Y
Yang
Kang
K
Bai
Yun
Y
D000230:Adenocarcinoma / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D019175:DNA Methylation; D004938:Esophageal Neoplasms / Q000235:genetics*; D005091:Exons; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D015854:Up-Regulation
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D000077022:Survivin; D016159:Tumor Suppressor Protein p53