Alpha2 macroglobulin-like is essential for liver development in zebrafish.
BACKGROUND
Alpha 2 Macroglobulin family members have been studied extensively with respect to their roles in physiology and human disease including innate immunity and Alzheimer's disease, but little is known about a possible role in liver development loss-of-function in model systems.


RESULTS
We report the isolation of the zebrafish alpha2 macroglobulin-like (A2ML) gene and its specific expression in the liver during differentiation. Morpholino-based knock-down of A2ML did not block the initial formation of the liver primordium, but inhibited liver growth and differentiation.


CONCLUSIONS
This report on A2ML function in zebrafish development provides the first evidence for a specific role of an A2M family gene in liver formation during early embryogenesis in a vertebrate.
Hong
Sung-Kook
SK
Laboratory of Molecular Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD, USA.
Dawid
Igor B
IB
D000818:Animals; D049109:Cell Proliferation; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D022781:Hepatocytes / Q000166:cytology; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009928:Organ Specificity; D038081:Organogenesis; D010179:Pancreas / Q000196:embryology / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D000511:alpha-Macroglobulins / Q000235:genetics* / Q000378:metabolism
D016376:Oligonucleotides, Antisense; D000511:alpha-Macroglobulins