title: Accumulation and effects of nodularin from a single and repeated oral doses of cyanobacterium Nodularia spumigena on flounder (Platichthys flesus L.).
abstract: Nodularin (NODLN) is a cyclic pentapeptide hepatotoxin produced by the cyanobacterium Nodularia spumigena, which occurs regularly in the Baltic Sea during the summer season. In this study flounder (Platichthys flesus L.) was orally exposed to NODLN either as a single dose or as three repeated doses 3 days apart. Liver and bile samples of the fish were taken 4 days after the last dose. Liver glutathione-S-transferase (GST) activity was also measured and the histopathology of the liver was investigated. The liver of the exposed fish was analyzed by liquid chromatography-mass spectrometry for NODLN concentration. The content of NODLN-like compounds in the bile was analyzed by enzyme-linked immunosorbent assay. NODLN exposure caused slightly incoherent liver architecture and degenerative cell changes in both groups. The mean liver GST activity was significantly higher in the repeatedly dosed flounders than in the singly dosed flounders or in the control. In conclusion, the significantly lower NODLN concentration and the increased GST activity in the liver of the repeatedly dosed flounders compared to the singly dosed flounders suggest that NODLN is rapidly detoxificated. The absence of NODLN glutathione conjugates and the low concentrations of NODLN-like compounds in the bile indicate that detoxification products disintegrate or they are rapidly excreted.
authors.affiliation: Finnish Game and Fisheries Research Institute, Helsinki, Finland. pekka.vuorinen@rktl.fi
authors.forename: Pekka J
authors.initials: PJ
authors.lastname: Vuorinen
authors.forename: Vesa O
authors.initials: VO
authors.lastname: Sipiä
authors.forename: Krister
authors.initials: K
authors.lastname: Karlsson
authors.forename: Marja
authors.initials: M
authors.lastname: Keinänen
authors.forename: Ambrose
authors.initials: A
authors.lastname: Furey
authors.forename: Orla
authors.initials: O
authors.lastname: Allis
authors.forename: Kevin
authors.initials: K
authors.lastname: James
authors.forename: Ulla
authors.initials: U
authors.lastname: Perttilä
authors.forename: Eija
authors.initials: E
authors.lastname: Rimaila-Pärnänen
authors.forename: Jussi A O
authors.initials: JA
authors.lastname: Meriluoto
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000633:toxicity*; D001646:Bile / Q000378:metabolism; D002853:Chromatography, Liquid; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005432:Flounder / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013058:Mass Spectrometry; D046932:Nodularia; D010456:Peptides, Cyclic / Q000008:administration & dosage / Q000633:toxicity*; D018675:Toxicity Tests
chemical_list: D001427:Bacterial Toxins; D010456:Peptides, Cyclic; C063998:nodularin; D005982:Glutathione Transferase