title: Identification of a thermo-regulated glutamine-binding protein from Yersinia pestis.
abstract: Here we present modeling and NMR spectroscopic evidence that the function of a Yersinia pestis pMT1 plasmid protein, designated as orf38, is most likely a glutamine binding protein. The modeling was homology-based at a very low level of sequence identity ( approximately 16%) and involved structural comparison of multiple templates, as well as template-substrate interaction analyses. Transferred nuclear Overhauser and saturation transfer difference experiments were used to characterize the binding of sugars and amino acids to orf38. The identification and characterization of an unknown protein function using the strategy presented here has applicability to a variety of research areas, including functional genomics and proteomics efforts.
authors.affiliation: Lawrence Livermore National Laboratory, Livermore, CA 94551, USA. monique.cosman@csueastbay.edu
authors.forename: Monique
authors.initials: M
authors.lastname: Cosman
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Pesavento
authors.forename: Adam
authors.initials: A
authors.lastname: Zemla
authors.forename: Peter T
authors.initials: PT
authors.lastname: Beernink
authors.forename: Rod
authors.initials: R
authors.lastname: Balhorn
authors.forename: Daniel
authors.initials: D
authors.lastname: Barsky
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005973:Glutamine / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D033903:Periplasmic Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D011487:Protein Conformation; D040681:Structural Homology, Protein; D015010:Yersinia pestis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D033903:Periplasmic Proteins; C037853:glutamine transport proteins; D005973:Glutamine