title: LYRM1, a novel gene promotes proliferation and inhibits apoptosis of preadipocytes.
abstract: OBJECTIVE
  To characterize a novel gene, Homo sapiens LYR motif containing 1 (LYRM1), that is highly expressed in omental adipose tissue of obese subjects.
  
  
  RESULTS
  RT-PCR and western blot analysis confirmed that both mRNA and protein levels of LYRM1 were higher in omental adipose tissue of obese subjects than in normal weight subjects. RT-PCR analysis demonstrated that LYRM1 expression is widely distributed, with the highest levels of expression occurring in adipose tissue. A fusion protein of LYRM1 and green fluorescent protein as well as western blot analysis were used to identify the subcellular localization of LYRM1 in the nucleus. Based on Oil red O staining and the expression profile of specific differentiation markers, ectopic LYRM1 expression was not found to significantly affect adipogenesis. MTT assays and cell cycle analysis showed that LYRM1 promotes preadipocyte proliferation, and data from annexin V-FITC and caspase-3 activity assays further determined that LYRM1 can inhibit apoptosis of preadipocytes.
  
  
  CONCLUSIONS
  By increasing cell proliferation and lowering the rate of apoptosis, LYRM1 has the potential to modulate the size of the preadipocyte pool and influence adipose tissue homeostasis.
authors.affiliation: Department of Pediatrics, Nanjing Maternity and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qiu
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Gao
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Chen
authors.forename: Xia
authors.initials: X
authors.lastname: Chi
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Zhang
authors.forename: Chen-Bo
authors.initials: CB
authors.lastname: Ji
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Zhao
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Li
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Tong
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Ni
authors.forename: Xi-Rong
authors.initials: XR
authors.lastname: Guo
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D019556:COS Cells; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*
chemical_list: D000906:Antibodies; D051017:Apoptosis Regulatory Proteins; C570850:LYR motif containing 1 protein, mouse; C538836:LYRM1 protein, human; D011506:Proteins