title: An improved tetracycline-inducible expression vector for Staphylococcus aureus.
abstract: The tetracycline-inducible expression vector pALC2073 allowed high level expression of the cloned sasG gene but repression by uninduced cells was leaky. The -10 box of the tetR promoter was mutated to the Bacillus subtitlis consensus, which resulted in complete repression of SasG protein expression. Anhydrotetracycline at 1.28 microg ml(-1) gave the same high level of induction that was obtained with pALC2073sasG using 160 ng ml(-1) tetracycline, the highest concentration that could be used without inhibiting bacterial growth. This variant of pALC2073 thus offers almost complete repression when uninduced and high levels of expression when induced.
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland.
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Corrigan
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Foster
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005822:Genetic Vectors / Q000187:drug effects; D008565:Membrane Proteins / Q000235:genetics; D009154:Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects; D014176:Protein Biosynthesis / Q000187:drug effects / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics*; D013752:Tetracycline / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C524374:SasG protein, Staphylococcus aureus; D013752:Tetracycline