title: Differential diagnosis of Taenia asiatica using multiplex PCR.
abstract: Taenia asiatica and T. saginata are frequently confused tapeworms due to their morphological similarities and sympatric distribution in Asian regions. To resolve this problem, a high-resolution multiplex PCR assay was developed to distinguish T. asiatica infections from infection with other human Taenia tapeworms. For molecular characterization, the species specificity of all materials used was confirmed by sequencing of the cox1 gene. Fifty-two samples were analyzed in this study, comprising 20 samples of T. asiatica genomic DNA from China, Korea, and the Philippines; 24 samples of T. saginata from Belgium, Chile, China, Ethiopia, France, Indonesia, Korea, Laos, the Philippines, Poland, Taiwan, Thailand, and Switzerland; and 10 samples of T. solium from Cape Verde, China, Honduras, and Korea. The diagnostic quality of the results obtained using PCR and species-specific primers designed from valine tRNA and NADH genes was equal to that based on the nucleotide sequencing of the cox1 gene. Using oligonucleotide primers Ta4978F, Ts5058F, Tso7421F, and Rev7915, the multiplex PCR assay was useful for the differentially diagnosing T. asiatica, T. saginata, and T. solium based on 706-, 629-, and 474-bp bands.
authors.affiliation: Department of Parasitology and Medical Research Institute, Chungbuk National University College of Medicine, Chungbuk, Republic of Korea.
authors.forename: Hyeong-Kyu
authors.initials: HK
authors.lastname: Jeon
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
authors.forename: Yoon
authors.initials: Y
authors.lastname: Kong
authors.forename: Jitra
authors.initials: J
authors.lastname: Waikagul
authors.forename: Bounnaloth
authors.initials: B
authors.lastname: Insisiengmay
authors.forename: Han-Jong
authors.initials: HJ
authors.lastname: Rim
authors.forename: Keeseon S
authors.initials: KS
authors.lastname: Eom
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry* / Q000302:isolation & purification; D004272:DNA, Mitochondrial / Q000737:chemistry; D003937:Diagnosis, Differential; D005243:Feces / Q000469:parasitology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013621:Taenia / Q000145:classification* / Q000235:genetics; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D004272:DNA, Mitochondrial