Crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 6.
Adeno-associated viruses are being developed as vectors for gene therapy and have been used in a number of clinical trials. Vectors to date have been based on the type species AAV-2, the structure of which was published in 2002. There is growing interest in modulating the cellular tropism and immune neutralization of AAV-2 with variants inspired by the properties of other serotypes. Towards the determination of a structure for AAV type 6, this paper reports the high-yield production, purification, crystallization and preliminary diffraction studies of infectious AAV-6 virions. The crystals diffracted to 3.2 A resolution using synchrotron radiation. The most promising crystal form belonged to space group R3 and appeared to be suitable for initial structure determination.
Xie
Qing
Q
Department of Biochemistry and Molecular Biology, School of Medicine, Oregon Health and Science University, Portland, OR 97239-3098, USA.
Ongley
Heather M
HM
Hare
Joan
J
Chapman
Michael S
MS
D000818:Animals; D003460:Crystallization; D000229:Dependovirus / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D014771:Virion / Q000737:chemistry* / Q000302:isolation & purification; D014961:X-Ray Diffraction