title: Mipu1, a novel rat zinc-finger protein, inhibits transcriptional activities of AP-1 and SRE in mitogen-activated protein kinase signaling pathway.
abstract: Mipu1 is a novel rat gene recently identified in our lab. Mipu1 cDNA contains a 1,824 bp open reading frame (ORF) and encoded a 608 amino acid protein with an N-terminal Krüppel-associated box (KRAB) domain and classical zinc finger C(2)H(2) motifs in the C-terminus. Mipu1 protein is located in the nuclei. Fused to Gal-4 DNA-binding domain and cotransfected with pG5-luc, Mipu1 played a transcriptional suppressive effect. Deletion analysis with a series of truncated fusion proteins indicated that the KRAB motif was a basal repression domain. Overexpression of Mipu1 in H9c2 myogenic cells inhibited the transcriptional activities of SRE and AP-1. RNAi of Mipu1 in H9c2 myogenic cells activated the transcriptional activities of SRE and AP-1. These results suggested that Mipu1 protein might act as a transcriptional repressor in mitogen-activated protein kinase (MAPK) signaling pathway to mediate cellular functions.
authors.affiliation: Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan, 410008, People's Republic of China.
authors.forename: Guiliang
authors.initials: G
authors.lastname: Wang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zuo
authors.forename: Can
authors.initials: C
authors.lastname: Yuan
authors.forename: Yazhu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D016335:Zinc Fingers
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C520710:Mipu1 protein, rat; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D020928:Mitogen-Activated Protein Kinases