title: A novel thermoacidophilic cellulase from Alicyclobacillus acidocaldarius.
abstract: A novel cellulase was isolated from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius ATCC27009 grown in medium containing carboxymethylcellulose. The enzyme is a glycosylated monomer of 56.2 kDa, relatively thermostable, with optimal pH and temperature of 4.0 and 65 degrees C, respectively. Enzymatic assays on several polysaccharides demonstrated that CelG was specific for carboxymethylcellulose.
authors.affiliation: Institute of Protein Biochemistry - C.N.R., Via P. Castellino 111, 80131, Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Morana
authors.forename: A
authors.initials: A
authors.lastname: Esposito
authors.forename: L
authors.initials: L
authors.lastname: Maurelli
authors.forename: G
authors.initials: G
authors.lastname: Ruggiero
authors.forename: E
authors.initials: E
authors.lastname: Ionata
authors.forename: M
authors.initials: M
authors.lastname: Rossi
authors.forename: F
authors.initials: F
authors.lastname: La Cara
mesh_terms: D002480:Cellulase / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D016936:Gram-Positive Endospore-Forming Bacteria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D013696:Temperature; D014461:Ultracentrifugation
chemical_list: D002480:Cellulase