title: Stromal control of oncogenic traits expressed in response to the overexpression of GLI2, a pleiotropic oncogene.
abstract: Hedgehog signaling is often activated in tumors, yet it remains unclear how GLI2, a transcription factor activated by this pathway, acts as an oncogene. We show that GLI2 is a pleiotropic oncogene. The overexpression induces genomic instability and blocks differentiation, likely mediated in part by enhanced expression of the stem cell gene SOX2. GLI2 also induces transforming growth factor (TGF)B1-dependent transdifferentiation of foreskin and tongue, but not gingival fibroblasts into myofibroblasts, creating an environment permissive for invasion by keratinocytes, which are in various stages of differentiation having downregulated GLI2. Thus, upregulated GLI2 expression is sufficient to induce a number of the acquired characteristics of tumor cells; however, the stroma, in a tissue-specific manner, determines whether certain GLI2 oncogenic traits are expressed.
authors.affiliation: Cancer Research Institute, University of California San Francisco, San Francisco, CA 94143-0808, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Snijders
authors.forename: B
authors.initials: B
authors.lastname: Huey
authors.forename: S T
authors.initials: ST
authors.lastname: Connelly
authors.forename: R
authors.initials: R
authors.lastname: Roy
authors.forename: R C K
authors.initials: RC
authors.lastname: Jordan
authors.forename: B L
authors.initials: BL
authors.lastname: Schmidt
authors.forename: D G
authors.initials: DG
authors.lastname: Albertson
mesh_terms: D000293:Adolescent; D000328:Adult; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D005784:Gene Amplification / Q000502:physiology; D042822:Genomic Instability / Q000235:genetics; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D009857:Oncogenes / Q000502:physiology; D009928:Organ Specificity / Q000235:genetics; D017154:Stromal Cells / Q000378:metabolism / Q000502:physiology*; D015854:Up-Regulation / Q000235:genetics* / Q000502:physiology; D055815:Young Adult; D000075746:Zinc Finger Protein Gli2
chemical_list: C082593:GLI2 protein, human; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; D000075746:Zinc Finger Protein Gli2