UDP-Gal: N-acetylglucosamine beta 1-4 galactosyltransferase expressing live attenuated parasites as vaccine for visceral leishmaniasis.
As compared to cutaneous leishmaniasis, vaccination against visceral leishmaniasis (VL) has received limited attention. In this study, we demonstrate for the first time that an UDP-Galactose: N-acetylglucosamine beta 1-4 galactosyltransferase (GenBank Accession No. EF159943) expressing attenuated LD clonal population (A-LD) is able to confer protection against the experimental challenge with the virulent LD AG83 parasite. A-LD was also effective in established leishmania infection. The vaccinated animals showed both cell mediated (in vitro T-cell proliferation, and DTH response) and humoral responses (Th1 type). These results demonstrate the potential of the attenuated clones as an immunotherapeutic and immunoprophylactic agent against visceral leishmaniasis.
Bhaumik
Siddhartha Kumar
SK
Division of Infectious Disease and Immunology, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Kolkata, 700032, India.
Singh
Manoj Kumar
MK
Karmakar
Subir
S
De
Tripti
T
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology; D005700:Galactosyltransferases / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007728:Kupffer Cells / Q000378:metabolism / Q000469:parasitology; D007893:Leishmania donovani / Q000201:enzymology* / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000517:prevention & control*; D008099:Liver / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D016052:Protozoan Vaccines / Q000276:immunology*; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000469:parasitology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
D000953:Antigens, Protozoan; D016052:Protozoan Vaccines; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D005700:Galactosyltransferases