Acute effects of breakfasts containing alpha-lactalbumin, or gelatin with or without added tryptophan, on hunger, 'satiety' hormones and amino acid profiles.
Proteins are the most satiating macronutrients. Tryptophan (TRP) may contribute to the satiating effect, as it serves as a precursor for the anorexigenic neurotransmitter serotonin. To address the role of TRP in the satiating properties of dietary protein, we compared three different breakfasts, containing either alpha-lactalbumin (high in TRP), gelatin (low in TRP) or gelatin with added TRP (gelatin+TRP, high in TRP), on appetite. Twenty-four subjects (22-29 kg/m2; aged 19-37 years) received a subject-specific breakfast at t = 0 with 10, 55 and 35 % energy from protein, carbohydrate and fat respectively in a randomised, single-blind design. Hunger, glucagon-like peptide (GLP)-1, ghrelin, amino acid concentrations and energy intake during a subsequent lunch were determined. Suppression of hunger was stronger 240 min after the breakfast with alpha-lactalbumin compared with gelatin and gelatin+TRP. Total plasma amino acid concentrations were lower with alpha-lactalbumin compared with gelatin with or without TRP (from t = 180-240 min). TRP concentrations were higher after alpha-lactalbumin than after gelatin with or without TRP from t = 0-100 min, whereas from t = 100-240 min, TRP concentrations were lower after gelatin than after alpha-lactalbumin and gelatin+TRP. The plasma ratio of TRP to other large neutral amino acids (LNAA) was, only at t = 100 min, lower after gelatin+TRP than after the other breakfasts. Plasma amino acid responses, TRP concentrations and TRP:LNAA ratios were not correlated with hunger. GLP-1 and ghrelin concentrations were similar for all diets. Energy intake during a subsequent lunch was similar for all diets. Summarised, an alpha-lactalbumin breakfast suppresses hunger more than a gelatin or gelatin+TRP breakfast. This cannot be explained by (possible) differences found in TRP concentrations and TRP:LNAA ratios in the breakfasts and in plasma, as well as in circulating total amino acids, GLP-1 and ghrelin.
Nieuwenhuizen
Arie G
AG
Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht, Maastricht University, PO Box 616, Maastricht 6200 MD, The Netherlands. a.nieuwenhuizen@hb.unimaas.nl
Hochstenbach-Waelen
Ananda
A
Veldhorst
Margriet A B
MA
Westerterp
Klaas R
KR
Engelen
Mariëlle P K J
MP
Brummer
Robert-Jan M
RJ
Deutz
Nicolaas E P
NE
Westerterp-Plantenga
Margriet S
MS
D000328:Adult; D000596:Amino Acids / Q000097:blood; D019540:Area Under Curve; D004044:Dietary Proteins / Q000008:administration & dosage*; D005260:Female; D005780:Gelatin / Q000008:administration & dosage*; D052216:Glucagon-Like Peptide 1 / Q000097:blood; D006801:Humans; D006815:Hunger / Q000502:physiology*; D007768:Lactalbumin / Q000008:administration & dosage*; D008297:Male; D012044:Regression Analysis; D012528:Satiety Response; D016037:Single-Blind Method; D014364:Tryptophan / Q000008:administration & dosage* / Q000097:blood; D055815:Young Adult
D000596:Amino Acids; D004044:Dietary Proteins; D052216:Glucagon-Like Peptide 1; D014364:Tryptophan; D005780:Gelatin; D007768:Lactalbumin