Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos.
Agrobacterium-mediated transformation of wheat is becoming a viable alternative to the more established biolistic protocols. It offers advantages in terms of simple, low-copy-number integrations and can be applied with similar efficiencies to specific durum wheat and spring and winter bread wheat types varieties.
Wu
Huixia
H
Genetic Resources and Enhancement Unit, CIMMYT, Mexico DF, Mexico.
Doherty
Angela
A
Jones
Huw D
HD
D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D018014:Gene Transfer Techniques; D030821:Plants, Genetically Modified; D012639:Seeds / Q000196:embryology / Q000235:genetics* / Q000382:microbiology*; D014170:Transformation, Genetic / Q000235:genetics*; D014908:Triticum / Q000196:embryology / Q000235:genetics* / Q000382:microbiology*