title: The dynamics of protein and metal metabolism in acclimated and Cd-exposed freshwater crabs (Potamonautes warreni).
abstract: Climatic and man-made impacts induced dynamic molecular responses in the South African freshwater crab, Potamonautes warreni. Adult crabs exhibited MT-like protein, binding Cd (0.02micromolg(-1) wet mass+/-0.02), Cu (0.326micromolg(-1) wet mass+/-0.15), and Zn (0.534micromolg(-1) wet mass+/-0.20). The native protein binding Cd, Cu, and Zn showed a respective molecular mass (M) of 9.10kDa+/-1.74, 8.95kDa+/-1.66, and 9.32kDa+/-0.93. With exposure to 0.2mgCd(2+)l(-1) for up to 21 days in 50% of these crabs approximately 90% of Cd was bound to the MT-like protein component (8.54kDa+/-1.64), coinciding with a Zn-bound MT-like component (8.2kDa+/-1.54). Less than 10% were bound in the high M protein component, suggesting a protective function of the protein. In the remaining crabs metals were bound to protein (6.8kDa) with a predominant Cu-binding component.
authors.affiliation: University of Plymouth at Britannia Royal Naval College, Dartmouth, Devon TQ6 9EN, UK. ms@brnc.ac.uk
authors.forename: P-M M
authors.initials: PM
authors.lastname: Schuwerack
authors.forename: John W
authors.initials: JW
authors.lastname: Lewis
authors.forename: Peter
authors.initials: P
authors.lastname: Jones
mesh_terms: D000818:Animals; D015415:Biomarkers; D003386:Brachyura / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D003300:Copper / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism; D005260:Female; D005618:Fresh Water; D008099:Liver / Q000378:metabolism; D008297:Male; D008667:Metalloproteins / Q000737:chemistry / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D011485:Protein Binding; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015032:Zinc / Q000378:metabolism
chemical_list: D015415:Biomarkers; D008667:Metalloproteins; D008670:Metals; D014874:Water Pollutants, Chemical; D002104:Cadmium; D003300:Copper; D015032:Zinc