title: Removal of furanocoumarins in grapefruit juice by edible fungi.
abstract: Furanocoumarins (FCs) in the human diet irreversibly inhibit human cytochrome P450 3A4 (CYP 3A4) and are responsible for the "grapefruit/drug""interaction phenomenon. Previously, we reported that FCs in grapefruit juice (GFJ) bind to autoclaved Aspergillus niger, and this binding reduced the GFJ inhibition of CYP 3A4. However, A. niger is not an edible fungus, and thus, potentially similar binding by edible fungi was also characterized. In this study, autoclaved Morchella esculenta, an edible ascomycete, removed much of the FC content in GFJ, resulting in decreased inhibition of CYP 3A4 activity by the GFJ. Three other edible fungi, Monascus purpureus, Pleurotus sapidus, and Agaricus bisporus, were evaluated for their binding with two of the major FCs in GFJ, 6',7'-dihydroxybergamottin (DHB) and bergamottin (BM). These autoclaved edible fungi removed these FCs from GFJ, similar to M. esculenta, indicating that binding is a general, passive interaction between FCs and fungal hyphae. The removal of FCs was independent of pH in GFJ. Dried fungal material of M. esculenta was also effective in removing FCs from GFJ and occurred with GFJ samples prepared from both fresh grapefruit and GFJ concentrate.
authors.affiliation: United States Citrus and Subtropical Products Laboratory, Agricultural Research Service, United States Department of Agriculture, 600 Avenue S, Northwest, Winter Haven, Florida 33881, USA. kyung.myung@ars.usda.gov
authors.forename: Kyung
authors.initials: K
authors.lastname: Myung
authors.forename: Jan A
authors.initials: JA
authors.lastname: Narciso
authors.forename: John A
authors.initials: JA
authors.lastname: Manthey
mesh_terms: D001628:Beverages / Q000032:analysis*; D032083:Citrus paradisi / Q000737:chemistry*; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D065692:Cytochrome P-450 CYP3A Inhibitors; D004791:Enzyme Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D005658:Fungi / Q000378:metabolism*; D011564:Furocoumarins / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D065692:Cytochrome P-450 CYP3A Inhibitors; D004791:Enzyme Inhibitors; D011564:Furocoumarins; D051544:Cytochrome P-450 CYP3A