Measurement of benzophenones in human urine samples by stir bar sorptive extraction and thermal desorption-gas chromatography-mass spectrometry.
Determination of benzophenones (BPs) in human urine samples by stir bar sorptive extraction (SBSE) and thermal desorption (TD)-gas chromatography-mass spectrometry (GC-MS) is described. As analytes, BP, its metabolites benzhydrol (BP-OH) and 2-hydroxybenzophenone (2OH-BP), and its derivatives 2-hydroxy-4-methoxybenzophenone (BP-3) and 2-hydroxy-4-methoxy-4'-methylbenzophenone (BP-10) were selected. After enzymatic hydrolysis, a polydimethylsiloxane (PDMS) stir bar was placed in a urine sample diluted 1:1 with water and stirred for 60 min at room temperature. The limit of quantification (LOQ) of BPs is 0.2-0.5 ng ml(-1) (ppb). The method showed linearity over the calibration range (0.2-10 or 0.5-10 ng ml(-1)), and the correlation coefficients were equal to or higher than 0.993 for all of the analytes. The average recoveries of BPs were equal to or higher than 98.7% (RSD: 1.5-4.8%, n = 6).
Kawaguchi
Migaku
M
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa, Tokyo 142-8501, Japan.
Ito
Rie
R
Honda
Hidehiro
H
Endo
Naoyuki
N
Okanouchi
Noriya
N
Saito
Koichi
K
Seto
Yasuo
Y
Nakazawa
Hiroyuki
H
D001577:Benzophenones / Q000378:metabolism / Q000652:urine*; D002138:Calibration; D004798:Enzymes / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000592:standards; D006801:Humans; D006868:Hydrolysis
D001577:Benzophenones; D004798:Enzymes