A novel spectrophotometric method for the determination of aminophylline with boric acid in pharmaceutical and mixed serum samples.
This paper firstly describes a novel method to determine aminophylline (Ami) with boric acid (BA) by spectrophotometry. The study indicates that at pH 12.00 the absorbance of Ami decreases when BA is added. A simple, rapid, sensitive and reliable novel method based on the product of Ami and BA is obtained. Beer's law is obeyed in the range of Ami concentrations of 0.20-200 microg ml(-1). The equation of linear regression is A=-2.57309x10(-4)-0.00355C (microg ml(-1)), with a linear correlation coefficient of 0.9969 and RSD 0.28%. The method is successfully applied to the determination of Ami in pharmaceutical samples and mixed serum samples, and average recoveries were in the range of 97.1-105.9%.
Li
Quanmin
Q
College of Chemistry and Environmental Science, Henan Normal University, Henan Key Laboratory for Environmental Pollution Control, Xinxiang, Henan 453007, PR China. mercury6068@hotmail.com
Zhang
Tiantian
T
Lv
Wenying
W
D000042:Absorption; D000628:Aminophylline / Q000032:analysis* / Q000097:blood* / Q000737:chemistry; D016477:Artifacts; D001888:Boric Acids / Q000737:chemistry*; D002021:Buffers; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors
D001888:Boric Acids; D002021:Buffers; D004364:Pharmaceutical Preparations; D000628:Aminophylline; C032688:boric acid