Micro-dissection of rat brain for RNA or protein extraction from specific brain region.
Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.
Chiu
Kin
K
Laboratory of Neurodegenerative Diseases, Department of Anatomy, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
Lau
Wui Man
WM
Lau
Ho Tak
HT
So
Kwok-Fai
KF
Chang
Raymond Chuen-Chung
RC
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000601:surgery; D003342:Corpus Striatum / Q000378:metabolism; D005625:Frontal Lobe / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D042282:Microdissection / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D009830:Olfactory Bulb / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification*; D051381:Rats; D013048:Specimen Handling / Q000379:methods*
D009419:Nerve Tissue Proteins; D012313:RNA