Quantitative rt-PCR methods for investigation of low copy potassium channel gene expression in native murine arteries.
Voltage-gated K+ channels (K(V) channels) are encoded by the KCNx gene family and have such a wide range of properties that it is necessary to identify the precise expression profile that is instrumental in governing the electrical phenotype of a cell and its response to extrinsic factors. Real-time quantitative RT-PCR methodology has been developed and validated for specific RNA species in vascular smooth muscle cells. We have shown that most of the KCNA gene family, encoding the major K(V)alpha1 subunits, was markedly up-regulated in the resistance artery compared to the thoracic aorta, in line with reported patch-clamp recordings. Thus quantitative real-time RT-PCR data can be translated into physiological response.
Cheong
Alex
A
Institute of Membrane and Systems Biology, University of Leeds, UK.
Fountain
Samuel J
SJ
Beech
David J
DJ
D000818:Animals; D001158:Arteries / Q000502:physiology*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D015221:Potassium Channels / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051637:Shaker Superfamily of Potassium Channels / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D015221:Potassium Channels; D051637:Shaker Superfamily of Potassium Channels; D012313:RNA