Absolute choline concentration measured by quantitative proton MR spectroscopy correlates with cell density in meningioma.
BACKGROUND
This study was aimed to investigate the relationship between quantitative proton magnetic resonance spectroscopy (1H-MRS) and pathological changes in meningioma.


METHODS
Twenty-two meningioma cases underwent single voxel 1H-MRS (point-resolved spectroscopy sequence, repetition time/echo time = 2,000 ms/68, 136, 272 ms). Absolute choline (Cho) concentration was calculated using tissue water as the internal reference and corrected according to intra-voxel cystic/necrotic parts. Pathological specimens were stained with MIB-1 antibody to measure cell density and proliferation index. Correlation analysis was performed between absolute Cho concentration and cell density and MIB-1 labeled proliferation index.


RESULTS
Average Cho concentration of all meningiomas before correction was 2.95 +/- 0.86 mmol/kg wet weight. It was increased to 3.23 +/- 1.15 mmol/kg wet weight after correction. Average cell density of all meningiomas was 333 +/- 119 cells/HPF, and average proliferation index was 2.93 +/- 5.72%. A linear, positive correlation between cell density and Cho concentration was observed (r = 0.650, P = 0.001). After correction of Cho concentration, the correlation became more significant (r = 0.737, P < 0.001). However, no significant correlation between Cho concentration and proliferation index was found. There seemed to be a positive correlation trend after correction of Cho concentration but did not reach significant level.


CONCLUSIONS
Absolute Cho concentration, especially Cho concentration corrected according to intra-voxel cystic/necrotic parts, reflects cell density of meningioma.
Yue
Qiang
Q
Department of Neurosurgery, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba Science City, Ibaraki, 305-8575, Japan.
Shibata
Yasushi
Y
Isobe
Tomonori
T
Anno
Izumi
I
Kawamura
Hiraku
H
Gong
Qi-Yong
QY
Matsumura
Akira
A
D000328:Adult; D000368:Aged; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000737:chemistry* / Q000473:pathology*; D002452:Cell Count; D002794:Choline / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016014:Linear Models; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008579:Meningioma / Q000737:chemistry* / Q000473:pathology*; D008875:Middle Aged; D011522:Protons; D055815:Young Adult
D011522:Protons; D002794:Choline