Analysis of fluorotelomer alcohols and perfluorinated sulfonamides in biotic samples by liquid chromatography-atmospheric pressure photoionization mass spectrometry.
A quantitative analytical method was developed to simultaneously detect fluorotelomer alcohols (6:2 FTOH, 8:2 FTOH and 10:2 FTOH) and polyfluorinated sulfonamides (perfluoro-1-octanesulfonamide (FOSA) and N-methylperfluoro-1-octanesulfonamide (NMeFOSA)) in biotic samples with liquid chromatography-atmospheric pressure photoionization mass spectrometry (LC-APPI-MS/MS). APPI mass spectra for FOSA and NMeFOSA showed that the major ionization mechanism was not photoionization, whereas for the FTOHs it was photoionization. For FTOHs, a [M+O(2)](-) ion was generated with a similar response as the deprotonated molecular ion [M-H](-). We demonstrated that FTOHs, FOSA and NMeFOSA can be measured in various biota samples using APPI with a minimized matrix effect. Using APPI, the linear response range for the FTOHs was 0-1,000 ng/mL (r(2)>0.9997), and for FOSA and NMeFOSA ranged from 0 to 250 ng/mL (r(2)>0.995). The instrument and method detect limits ranged from 0.16 to 0.63 pg and below 1 ng/g wet weight (w.w.), respectively. For the overall method applied to the test matrices, recovery efficiencies ranged from 73 to 102% for egg homogenate and 89-100% for liver tissue. The present study demonstrates for the first time that a far more response and sensitive approach for the detection and quantification of FTOHs and polyfluorinated sulfonamides is possible using APPI as opposed to electrospray ionization.
Chu
Shaogang
S
Wildlife and Landscape Science Directorate, Science and Technology Branch, Environment Canada, National Wildlife Research Centre, Carleton University, Ottawa, ON K1A 0H3, Canada.
Letcher
Robert J
RJ
D000818:Animals; D001274:Atmospheric Pressure; D002853:Chromatography, Liquid / Q000379:methods*; D005466:Fluorocarbons / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D013449:Sulfonamides / Q000032:analysis*; D001503:Ursidae
D005466:Fluorocarbons; D013449:Sulfonamides