title: Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae.
abstract: A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties. In this study, we reconstructed the E. coli isoprenoid biosynthetic pathway in S. cerevisiae. Genes encoding the seven enzymatic steps of the pathway were cloned and expressed in S. cerevisiae. mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway.
authors.affiliation: Center for Microbial Biotechnology, DTU-Biosys, Building 223, 2800 Kgs Lyngby, Denmark. jema@bio.dtu.dk
authors.forename: Jérôme
authors.initials: J
authors.lastname: Maury
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Asadollahi
authors.forename: Kasper
authors.initials: K
authors.lastname: Møller
authors.forename: Michel
authors.initials: M
authors.lastname: Schalk
authors.forename: Anthony
authors.initials: A
authors.lastname: Clark
authors.forename: Luca R
authors.initials: LR
authors.lastname: Formenti
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D008148:Lovastatin / Q000494:pharmacology; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D013729:Terpenes; D008148:Lovastatin