Development and validation of a simple and rapid HPLC method for determination of pioglitazone in human plasma and its application to a pharmacokinetic study.
In this study, a new, simple, and reproducible high-performance liquid chromatographic method was developed for the determination of pioglitazone in human plasma. After liquid-liquid extraction with diethylether, samples were quantitated on a Nova-Pak C8 column using a mixture of acetonitrile-140mM K2HPO4 (40:60, v/v, pH = 4.45) as mobile phase with UV detection at 269 nm. The flow rate was set at 1.4 mL/min. Ethylparaben was used as internal standard and the total run time of analysis was approximately 7 min. The method was linear over the range of 25-1500 ng/mL of pioglitazone in plasma (r2 > 0.999). The within- and between-day precision values were in the range of 2.4-6.8%. The limit of quantitation of the method was 25 ng/mL. The method was successfully used to study the pharmacokinetics of pioglitazone in healthy volunteers.
Souri
Effat
E
Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Medical Sciences / University of Tehran, Tehran (14155-6451), Iran. souri@sina.tumbs.ac.ir
Jalalizadeh
Hassan
H
Saremi
Shahrooz
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D008297:Male; D000077205:Pioglitazone; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D045162:Thiazolidinediones / Q000097:blood* / Q000493:pharmacokinetics*
D045162:Thiazolidinediones; D000077205:Pioglitazone