title: Prospective study of infection and nephropathy due to BK and JC polyomavirus in 76 kidney transplant recipients.
abstract: BACKGROUND
  Nephropathy due to polyomavirus is usually diagnosed by renal biopsy after worsening of renal function. This is normally at an advanced stage of the disease.
  
  
  OBJECTIVE
  To study the early detection of the presence of BK and JC polyomavirus in urine by monthly real-time quantitative polymerase chain reaction (PCR) assay.
  
  
  METHODS
  The study included 76 kidney transplant recipients from cadaveric donors between August 2005 and July 2006 with a 1-year follow-up.
  
  
  RESULTS
  Viruria was positive in 31 patients (40.7%) and viremia in 7 (9.2%), three of whom (3.9%) developed nephropathy. After reduction of the immunosuppression, the viruria became negative in 32.0% and the viremia in 42.8% of the patients. Renal function (creatinine clearance, aMDRD) at 1 year was 49.2 mL/min/1.73 m(2) in the patients with nephropathy and 64.3 mL/min/1.73 m(2) in the others. One-year patient and graft survival was 96%. No patient lost the graft due to nephropathy.
  
  
  CONCLUSIONS
  The detection of BK and JC polyomavirus by protocolized PCR enabled an early diagnosis of nephropathy, preventing graft loss with good renal function at 1 year.
authors.affiliation: Nephrology Department, Hospital Universitario Carlos Haya, Malaga, Spain. verolopezjim@yahoo.es
authors.forename: V
authors.initials: V
authors.lastname: López
authors.forename: C
authors.initials: C
authors.lastname: Gutiérrez
authors.forename: D
authors.initials: D
authors.lastname: Burgos
authors.forename: M
authors.initials: M
authors.lastname: González Molina
authors.forename: M
authors.initials: M
authors.lastname: Cabello
authors.forename: E
authors.initials: E
authors.lastname: Sola
authors.forename: I
authors.initials: I
authors.lastname: Garcia
authors.forename: J
authors.initials: J
authors.lastname: Siles
authors.forename: P
authors.initials: P
authors.lastname: Florez
mesh_terms: D000328:Adult; D001739:BK Virus; D001706:Biopsy; D002102:Cadaver; D015331:Cohort Studies; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006650:Histocompatibility Testing; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007577:JC Virus; D016030:Kidney Transplantation / Q000009:adverse effects* / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000473:pathology*; D012189:Retrospective Studies; D014019:Tissue Donors; D014412:Tumor Virus Infections / Q000473:pathology*; D019562:Viral Load; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D007166:Immunosuppressive Agents