title: Analysis of the healing process in delayed tooth replantation after root canal filling with calcium hydroxide, Sealapex and Endofill: a microscopic study in rats.
abstract: The major concern in the therapeutics of tooth replantation refers to the occurrence of root resorption and different approaches have been proposed to prevent or treat these complications. The purpose of this study was to evaluate tissue response to delayed replantation of anterior rat teeth treated endodontically using calcium hydroxide, Sealapex, and Endofill without the placement of gutta-percha cones. Thirty rats had their right upper incisor extracted and maintained in dry storage for 60 min. After removal of the dental papilla, enamel organ, pulp tissue, and periodontal ligament remnants, the teeth were immersed in 2% sodium fluoride phosphate acidulated, pH 5.5, for 10 min. The root canals were dried with absorbent paper points and the teeth were assigned to three groups (n = 10) according to the filling material. Group I - calcium hydroxide and propyleneglycol paste, Group II - Sealapex, and Group III - Endofill. The sockets were irrigated with saline and the teeth were replanted. Replacement resorption, inflammatory resorption and ankylosis were observed in all groups. Although the occurrence of inflammatory resorption was less frequent in Group I, there were no statistically significant differences among the groups. It may be concluded that compared to the paste, filling the root canals with Sealapex and Endofill sealers without the placement of gutta-percha cones did not provide better results.
authors.affiliation: Department of Surgery and Integrated Clinics, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil.
authors.forename: Márcia Regina
authors.initials: MR
authors.lastname: Negri
authors.forename: Sônia Regina
authors.initials: SR
authors.lastname: Panzarini
authors.forename: Wilson Roberto
authors.initials: WR
authors.lastname: Poi
authors.forename: Celso Koogi
authors.initials: CK
authors.lastname: Sonoda
authors.forename: Jéssica Lemos
authors.initials: JL
authors.lastname: Gulinelli
authors.forename: Célia Tomiko Matida Hamata
authors.initials: CT
authors.lastname: Saito
mesh_terms: D000149:Acidulated Phosphate Fluoride / Q000627:therapeutic use; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D002126:Calcium Hydroxide / Q000627:therapeutic use*; D003238:Connective Tissue / Q000473:pathology; D003739:Dental Cementum / Q000473:pathology; D003786:Dental Pulp Cavity / Q000473:pathology; D003804:Dentin / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D007180:Incisor / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D010513:Periodontal Ligament / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012184:Retrograde Obturation / Q000379:methods*; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D012388:Root Canal Irrigants / Q000627:therapeutic use; D012391:Root Resorption / Q000209:etiology / Q000473:pathology; D012459:Salicylates / Q000627:therapeutic use*; D020254:Tooth Ankylosis / Q000209:etiology / Q000473:pathology; D014090:Tooth Replantation / Q000379:methods*; D020390:Tooth Socket / Q000473:pathology; D014945:Wound Healing / Q000502:physiology
chemical_list: D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D012459:Salicylates; C074417:Endo-Fill; C055601:Sealapex; D000149:Acidulated Phosphate Fluoride; D002126:Calcium Hydroxide