WtsE, an AvrE-family type III effector protein of Pantoea stewartii subsp. stewartii, causes cell death in non-host plants.
Pantoea stewartii subsp. stewartii (Pnss) causes Stewart's bacterial wilt of sweet corn and leaf blight of maize. The pathogenicity of Pnss depends on synthesis of extracellular polysaccharide and an Hrp type III secretion system. WtsE, a type III secreted effector protein, is essential for the virulence of Pnss on corn. It belongs to the AvrE family of effectors, which includes DspA/E from Erwinia amylovora and AvrE1 from Pseudomonas syringae. Previously, WtsE was shown to cause disease-associated cell death in its host plant, sweet corn. Here, we examine the biological activity of WtsE in several non-host plants. WtsE induced cell death in Nicotiana benthamiana, tobacco, beet and Arabidopsis thaliana when it was transiently produced in plant cells following agroinfiltration or translocated into plant cells from Pnss, Escherichia coli or Pseudomonas syringae pv. phaseolicola (Pph). WtsE-induced cell death in N. benthamiana, tobacco and beet resembled a hypersensitive response and in N. benthamiana it was delayed by cycloheximide. Interestingly, WtsE strongly promoted the growth of Pnss in N. benthamiana prior to the onset of cell death. Deletion derivatives of WtsE that failed to induce cell death in N. benthamiana and tobacco also did not complement wtsE mutants of Pnss for virulence in sweet corn, indicating a correlation between the two activities. WtsE also induced cell death in A. thaliana, where it suppressed basal defences induced by Pph. Thus, WtsE has growth-promoting, defence-suppressing and cell death-inducing activities in non-host plants. Expression of WtsE also prevented the growth of yeast, possibly due to an innate toxicity to eukaryotic cells.
Ham
Jong Hyun
JH
Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH 43210, USA.
Majerczak
Doris
D
Ewert
Sophie
S
Sreerekha
Mysore-Venkatarau
MV
Mackey
David
D
Coplin
David
D
D017360:Arabidopsis / Q000166:cytology / Q000254:growth & development / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D027461:Beta vulgaris / Q000166:cytology / Q000254:growth & development / Q000382:microbiology; D016923:Cell Death / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D020636:Pantoea / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D059828:Plant Cells; D063245:Plant Development; D010944:Plants / Q000382:microbiology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D014026:Nicotiana / Q000166:cytology / Q000254:growth & development / Q000382:microbiology; D014170:Transformation, Genetic; D014774:Virulence / Q000187:drug effects
D001426:Bacterial Proteins; D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide