title: "HOOF-Print" genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale.
abstract: We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus population genetic data to help discriminate among microbial isolates such as Brucella.
authors.affiliation: CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal. claudia_almendra@mail.icav.up.pt
authors.forename: Cláudia
authors.initials: C
authors.lastname: Almendra
authors.forename: Teresa Luísa
authors.initials: TL
authors.lastname: Silva
authors.forename: Albano
authors.initials: A
authors.lastname: Beja-Pereira
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Ferreira
authors.forename: Lénia
authors.initials: L
authors.lastname: Ferrão-Beck
authors.forename: Maria Inácia Corrêa
authors.initials: MI
authors.lastname: de Sá
authors.forename: Betsy J
authors.initials: BJ
authors.lastname: Bricker
authors.forename: Gordon
authors.initials: G
authors.lastname: Luikart
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D002002:Brucella / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017347:Brucella melitensis / Q000235:genetics*; D002006:Brucellosis / Q000382:microbiology; D005798:Genes, Bacterial; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D018598:Minisatellite Repeats; D008957:Models, Genetic; D010802:Phylogeny; D011174:Portugal