Oxygen-mediated regulation of biofilm development is controlled by the alternative sigma factor sigma(B) in Staphylococcus epidermidis.
Using a modified rotating-disk reactor to sparge oxygen to Staphylococcus epidermidis cultures, we found that oxygen negatively regulates biofilm development by influencing the activity of sigma(B). Under anaerobic conditions, increased sigma(B) activity activates icaADBC, which encodes enzymes responsible for polysaccharide intercellular adhesin synthesis, by repressing transcription of the negative regulator icaR.
Cotter
John J
JJ
UCD School of Chemical and Bioprocess Engineering, Engineering and Materials Science Centre, University College Dublin, Belfield, Dublin 4, Ireland.
O'Gara
James P
JP
Mack
Dietrich
D
Casey
Eoin
E
D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D010100:Oxygen / Q000378:metabolism*; D012808:Sigma Factor / Q000502:physiology*; D013212:Staphylococcus epidermidis / Q000502:physiology*
D001426:Bacterial Proteins; C079011:SigB protein, Bacteria; D012808:Sigma Factor; D010100:Oxygen