Simultaneous extraction and clean-up of polychlorinated biphenyls and their metabolites from small tissue samples using pressurized liquid extraction.
A pressurized liquid extraction-based method for the simultaneous extraction and in situ clean-up of polychlorinated biphenyls (PCBs), hydroxylated (OH)-PCBs and methylsulfonyl (MeSO(2))-PCBs from small (<0.5 g) tissue samples was developed and validated. Extraction of a laboratory reference material with hexane-dichloromethane-methanol (48:43:9, v/v) and Florisil as fat retainer allowed an efficient recovery of PCBs (78-112%; RSD: 13-37%), OH-PCBs (46+/-2%; RSD: 4%) and MeSO(2)-PCBs (89+/-21%; RSD: 24%). Comparable results were obtained with an established analysis method for PCBs, OH-PCBs and MeSO(2)-PCBs.
Kania-Korwel
Izabela
I
Department of Occupational and Environmental Health, Collage of Public Health, University of Iowa, Iowa City, IA 52242, USA.
Zhao
Hongxia
H
Norstrom
Karin
K
Li
Xueshu
X
Hornbuckle
Keri C
KC
Lehmler
Hans-Joachim
HJ
D000818:Animals; D001140:Aroclors / Q000008:administration & dosage; D005591:Chemical Fractionation / Q000379:methods*; D020111:Chlorodiphenyl (54% Chlorine) / Q000008:administration & dosage; D016014:Linear Models; D008099:Liver / Q000737:chemistry*; D008297:Male; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000302:isolation & purification*; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry
D001140:Aroclors; D012997:Solvents; D020111:Chlorodiphenyl (54% Chlorine); C027423:aroclor 1242; D011078:Polychlorinated Biphenyls