title: [Effects of Radix Ginseng and Radix Ophiopogonis extract (SMF) on protein S-nitrosylation in ischemic myocardial tissue].
abstract: OBJECTIVE
  To observe the effect of Radix Ginseng and Radix Ophiopogonis extract (SMF) on protein S-nitrosylation in rats myocardial with ischemia/reperfusion injury (MI/RI).
  
  
  METHODS
  Myocardial ischemia/reperfusion in rats were produced by occlusion of the left anterior descending coronary artery. To study the cardioprotective effects of SMF on the acute MI/RI rats, the serum levels of creatine kinase (CK), lactate dehydrogenase (LDH), and nitric oxide (NO) were determined. The change of the expression of endothelial nitric oxide synthase (eNOS) was detected by Western blot. The content of related S-nitrosylation proteins in myocardial tissue was measured by Biotin-Switch method.
  
  
  RESULTS
  SMF significantly decreased the serum levels of CK and LDH as well as increased the serum levels of NO and the expression of eNOS in myocardial tissue. The contents of S-nitrosylation proteins were significantly increased from (4.42 +/- 0.60) micromol x g(-1) to (8.78 +/- 1.37) micromol x g(-1). The molecular weight of the majority S-nitrosylation proteins were in the range of 90 x 10(3)-117 x 10(3).
  
  
  CONCLUSIONS
  Increased expression of eNOS and NO induced by SMF may activate S-nitrosylation of many proteins in rat hearts. The change of the activities or functions of those proteins by S-nitrosylation may be an important mechanism for myocardial protective effects of SMF.
authors.affiliation: Zhejiang University, Department of Chinese Medicine Science & Engineering, Hangzhou 310058, China.
authors.forename: Jin-hong
authors.initials: JH
authors.lastname: Feng
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shi
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yi-yu
authors.initials: YY
authors.lastname: Cheng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003402:Creatine Kinase / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D017202:Myocardial Ischemia / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009569:Nitric Oxide / Q000097:blood; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D015538:Nitrosation / Q000187:drug effects; D009603:Nitroso Compounds / Q000378:metabolism*; D005894:Panax / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D009603:Nitroso Compounds; D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase; D052250:Nitric Oxide Synthase Type III; D003402:Creatine Kinase