title: Isolation and expansion of equine umbilical cord-derived matrix cells (EUCMCs).
abstract: Stem cells from extra-embryonic sources can be obtained by non-invasive procedures. We have standardized a method for the expansion of equine umbilical cord-derived matrix cells (EUCMCs) for potential therapy. EUCMCs were isolated from the umbilical cord of five mares immediately after delivery. For expansion, cells were grown in alpha-MEM and MSCBM. Moreover, to measure the effect of growth factor supplementation, epidermal growth factor (EGF) was added to alpha-MEM. alpha-MEM and MSCBM media performed similarly in terms of population doubling and CFU number value. EGF supplementation of alpha-MEM determined a significant increase of the population doubling value. EGF supplementation did not affect the adipogenic and chondrogenic differentiation while bone nodule sizes an increased with the osteogenic protocol. Both alpha-MEM and MSCBM can be used to cultivate EUCMCs. alpha-MEM supplemented with EGF might represent an advantage for EUCMCs expansion. The results could be useful in choosing the culture medium since alpha-MEM is more cost-effective than MSCBM.
authors.affiliation: Department of Veterinary Anatomy, Biochemistry and Physiology, University of Pisa, Pisa, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Passeri
authors.forename: Francesca
authors.initials: F
authors.lastname: Nocchi
authors.forename: Roberta
authors.initials: R
authors.lastname: Lamanna
authors.forename: Simone
authors.initials: S
authors.lastname: Lapi
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Miragliotta
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Giannessi
authors.forename: Francesca
authors.initials: F
authors.lastname: Abramo
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Stornelli
authors.forename: Micheletino
authors.initials: M
authors.lastname: Matarazzo
authors.forename: Daniele
authors.initials: D
authors.lastname: Plenteda
authors.forename: Patrizia
authors.initials: P
authors.lastname: Urciuoli
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Scatena
authors.forename: Alessandra
authors.initials: A
authors.lastname: Coli
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006736:Horses; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D003470:Culture Media; D004815:Epidermal Growth Factor