Chapter 10. Methods for evaluating uteroplacental angiogenesis and their application using animal models.
In this chapter, we present some of the compelling evidence confirming the importance of placental angiogenesis to fetal growth and development in normal and compromised pregnancies. We then describe the methodology that has been used to evaluate placental angiogenesis throughout pregnancy, including both the practical methods used to obtain reliable samples of the placental microcirculation as well as computerized methods used to analyze and reconstruct it. We then briefly describe the changes in placental angiogenesis and function in the models of normal and compromised pregnancy that we have used, and conclude with what we have learned from these studies and what we believe are the larger questions remaining.
Borowicz
Pawel P
PP
Center for Nutrition and Pregnancy, and Department of Animal Sciences, North Dakota State University, Fargo, North Dakota, USA.
Hafez
Shireen
S
Redmer
Dale A
DA
Reynolds
Lawrence P
LP
D000818:Animals; D005260:Female; D008954:Models, Biological; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010920:Placenta / Q000098:blood supply*; D021041:Placental Circulation / Q000502:physiology; D011247:Pregnancy; D011270:Pregnancy, Animal; D012756:Sheep; D014599:Uterus / Q000098:blood supply*