title: Mode of action of the immunostimulatory effect of collagen from jellyfish.
abstract: We have previously demonstrated that collagen from jellyfish simulated immunoglobulin and cytokine production by human-human hybridoma line HB4C5 cells and by human peripheral blood lymphocytes (hPBL). The mode of action of the collagen as an immunostimulatory factor was investigated. The expression levels of immunoglobulin mRNAs in HB4C5 cells, and those of tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and transforming growth factor (TGF)-beta in hPBL were up-regulated by jellyfish collagen. In addition, this collagen activated IgM production by transcription-suppressed HB4C5 cells that had been treated with actinomycin D. This collagen also enhanced IgM production by translation-suppressed HB4C5 cells that had been treated with sodium fluoride, but was ineffective in accelerating IgM production by HB4C5 cells treated with cycloheximide. Moreover, the intracellular IgM level in HB4C5 cells treated with the post-translation inhibitor, monensin, was increased by this collagen. These results suggest that collagen from jellyfish stimulated not only the transcription activity, but also the translation activity for enhanced immunoglobulin and cytokine production.
authors.affiliation: Center for Marine Environmental Studies (CMES), Ehime University, Japan.
authors.forename: Sogo
authors.initials: S
authors.lastname: Nishimoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Goto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Morishige
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Shiraishi
authors.forename: Mikiharu
authors.initials: M
authors.lastname: Doi
authors.forename: Koichi
authors.initials: K
authors.lastname: Akiyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamauchi
authors.forename: Takuya
authors.initials: T
authors.lastname: Sugahara
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002460:Cell Line; D003094:Collagen / Q000276:immunology* / Q000302:isolation & purification; D016207:Cytokines / Q000235:genetics; D006801:Humans; D007075:Immunoglobulin M / Q000235:genetics; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D037401:Scyphozoa / Q000737:chemistry*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D007075:Immunoglobulin M; D012333:RNA, Messenger; D003094:Collagen