title: Additive anti-hyperalgesia of electroacupuncture and intrathecal antisense oligodeoxynucleotide to interleukin-1 receptor type I on carrageenan-induced inflammatory pain in rats.
abstract: Accumulating evidence shows that spinal interleukin-1beta (IL-1beta) plays a critical role in inflammatory pain. Electroacupuncture (EA) can effectively attenuate inflammatory hyperalgesia both in clinical practices and experimental studies. However, little is known about the relationship between spinal IL-1beta and EA analgesia. The present study was designed to evaluate the effects of EA and antisense oligodeoxynucleotide (ODN) to IL-1 receptor type I (IL-1RI) on carrageenan-induced thermal hyperalgesia and the expression of IL-1beta as well as IL-1RI. It was demonstrated that carrageenan induced marked thermal hyperalgesia in the injected paw, hence making paw withdrawal latency (PWL) decrease to 3.47+/-0.31 s at 180 min post-injection. Nevertheless, when EA was administered for 30 min at 180 min post-carrageenan injection, the PWLs were significantly increased between 10 and 90 min following the beginning of EA treatment and peaked at 30 min to 5.91+/-0.61 s. And also EA partly reversed the elevation of IL-1beta and IL-1RI expression induced by carrageenan. Down-regulation of IL-1RI expression by repeated intrathecal antisense ODN (50 microg/10 microl) significantly increased the mean PWL up to 5.75+/-0.15 s in 180-300 min post-carrageenan injection. Additionally, when the combination of EA with antisense ODN was used, thermal hyperalgesia was further alleviated than EA or antisense ODN alone, with a maximum PWL of 7.66+/-0.50 s at 30 min post the beginning of EA treatment. The results suggested an involvement of the spinal IL-1beta/IL-1RI system in EA-induced anti-hyperalgesia in inflammatory pain.
authors.affiliation: Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China. minrainsong@163.com
authors.forename: Ming-Juan
authors.initials: MJ
authors.lastname: Song
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Wang
authors.forename: Gen-Cheng
authors.initials: GC
authors.lastname: Wu
mesh_terms: D000818:Animals; D002351:Carrageenan; D015536:Down-Regulation; D015671:Electroacupuncture; D006358:Hot Temperature; D006930:Hyperalgesia / Q000139:chemically induced / Q000628:therapy*; D007278:Injections, Spinal; D053583:Interleukin-1beta / Q000378:metabolism; D008027:Light; D008297:Male; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage*; D010146:Pain / Q000139:chemically induced; D059408:Pain Management; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D053573:Receptors, Interleukin-1 Type I / Q000235:genetics* / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D053573:Receptors, Interleukin-1 Type I; D002351:Carrageenan