Glorund interactions in the regulation of gurken and oskar mRNAs.
Precise temporal and spatial regulation of gene expression during Drosophila oogenesis is essential for patterning the anterior-posterior and dorsal-ventral body axes. Establishment of the anterior-posterior axis requires posterior localization and translational control of both oskar and nanos mRNAs. Establishment of the dorsal-ventral axis depends on the precise restriction of gurken mRNA and protein to the dorsal-anterior corner of the oocyte. We have previously shown that Glorund, the Drosophila hnRNP F/H homolog, contributes to anterior-posterior axis patterning by regulating translation of nanos mRNA, through a direct interaction with its 3' untranslated region. To investigate the pleiotropy of the glorund mutant phenotype, which includes dorsal-ventral and nuclear morphology defects, we searched for proteins that interact with Glorund. Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint and plays a role both in mRNA localization and nurse cell chromosome organization, probably by regulating alternative splicing of ovarian tumor. We propose that Glorund is a component of multiple protein complexes and functions both as a translational repressor and splicing regulator for anterior-posterior and dorsal-ventral patterning.
Kalifa
Yossi
Y
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Armenti
Stephen T
ST
Gavis
Elizabeth R
ER
D017398:Alternative Splicing; D000818:Animals; D019521:Body Patterning / Q000502:physiology; D002875:Chromosomes / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D034541:Heterogeneous-Nuclear Ribonucleoprotein Group F-H / Q000235:genetics / Q000378:metabolism*; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism; D009154:Mutation; D009865:Oocytes / Q000502:physiology; D009866:Oogenesis / Q000502:physiology; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016211:Transforming Growth Factor alpha / Q000235:genetics / Q000378:metabolism*; D020506:Untranslated Regions
D029721:Drosophila Proteins; C509827:Glo protein, Drosophila; D020662:Guanine Nucleotide Exchange Factors; D034541:Heterogeneous-Nuclear Ribonucleoprotein Group F-H; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C484363:Hfp protein, Drosophila; C487427:Hrb27C protein, Drosophila; D012333:RNA, Messenger; D016211:Transforming Growth Factor alpha; D020506:Untranslated Regions; C083730:grk protein, Drosophila; C091675:osk protein, Drosophila