Detection of miR-106a in gastric carcinoma and its clinical significance.
BACKGROUND
MicroRNAs (miRNAs) play important roles in carcinogenesis. miRNA-106a (miR-106a) has oncogenic activity in humans, and often has altered expression. The clinical significance of miR-106a in the diagnosis of gastric carcinoma is poorly understood.


METHODS
The level of miR-106a in 55 gastric carcinoma and 17 non-tumor tissues was quantified by real-time reverse transcriptase-polymerase chain reaction, and the relationship between miR-106a level and clinical and pathological factors was explored.


RESULTS
The level of miR-106a in cancer tissues was significantly higher than that in non-tumor tissues, with an average 1.625-fold increase. miR-106a level was significantly associated with tumor stage, size and differentiation; lymphatic and distant metastasis; and invasion (P<0.01). The altered expression of miR-106a was confirmed in gastric cancer cell lines.


CONCLUSIONS
miR-106a may be a potential biomarker in the diagnosis of gastric carcinoma.
Xiao
Bingxiu
B
Ningbo University, School of Medicine, Ningbo 315211, China. xiaobingxiu@nbu.edu.cn
Guo
Junming
J
Miao
Ying
Y
Jiang
Zhen
Z
Huan
Rong
R
Zhang
Yuanyuan
Y
Li
Dong
D
Zhong
Jiuchang
J
D015415:Biomarkers / Q000032:analysis; D045744:Cell Line, Tumor; D050687:E2F1 Transcription Factor / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics; D009367:Neoplasm Staging; D016160:Retinoblastoma Protein / Q000235:genetics; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D015854:Up-Regulation
D015415:Biomarkers; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D035683:MicroRNAs; D016160:Retinoblastoma Protein