title: Enzymes containing porous polymersomes as nano reaction vessels for cascade reactions.
abstract: Polystyrene(40)-b-poly(isocyanoalanine(2-thiophen-3-yl-ethyl)amide)(50) (PS-PIAT) polymersomes have the unique property of being sufficiently porous to allow diffusion of small (organic) substrates while retaining large biomolecules such as enzymes inside. Herein we report on the encapsulation and protection of glucose oxidase (GOx) and horse radish peroxidase (HRP) in PS-PIAT polymersomes and the successful employment of these functionalised nanoreactors in a cascade reaction. The demonstrated concept allows for further application in other enzymatic cascade reactions, bio-organic hybrid systems and biosensing devices.
authors.affiliation: Molecular Materials, Institute for Molecules and Materials, University of Nijmegen, Toernooiveld 1, Nijmegen, Gelderland, Netherlands 6500 GL.
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Kuiper
authors.forename: Madhavan
authors.initials: M
authors.lastname: Nallani
authors.forename: Dennis M
authors.initials: DM
authors.lastname: Vriezema
authors.forename: Jeroen J L M
authors.initials: JJ
authors.lastname: Cornelissen
authors.forename: Jan C M
authors.initials: JC
authors.lastname: van Hest
authors.forename: Roeland J M
authors.initials: RJ
authors.lastname: Nolte
authors.forename: Alan E
authors.initials: AE
authors.lastname: Rowan
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D005949:Glucose Oxidase / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007097:Imines / Q000737:chemistry*; D036103:Nanotechnology; D011137:Polystyrenes / Q000737:chemistry*; D016062:Porosity; D013997:Time Factors
chemical_list: D007097:Imines; D011137:Polystyrenes; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D000409:Alanine