title: Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
abstract: Kupffer cells are the resident macrophage population of the liver and have previously been implicated in the pathogenesis of hepatic ischemia-reperfusion injury (IRI). Kupffer cells are the major site of expression of hepatic heme oxygenase-1 (HO-1), which has been shown to have anti-inflammatory actions and to protect animals and cells from oxidative injury. Kupffer cells and circulating monocytes were selectively ablated using liposomal clodronate (LC) in the CD11b DTR mouse before induction of hepatic ischemia. Kupffer cell depletion resulted in loss of HO-1 expression and increased susceptibility to hepatic IRI, whereas ablation of circulating monocytes did not affect IRI phenotype. Targeted deletion of HO-1 rendered mice highly susceptible to hepatic IRI. In vivo, HO-1 deletion resulted in pro-inflammatory Kupffer cell differentiation characterized by enhanced Ly6c and MARCO (macrophage receptor with collagenous structure) expression as well as decreased F4/80 expression, mirrored by an expansion in immature circulating monocytes. In vitro, HO-1 inhibition throughout macrophage differentiation led to increased cell numbers, and pro-inflammatory Ly6c+ CD11c- F4/80- phenotype. These data support a critical role for tissue-resident macrophages in homeostasis following ischemic injury, and a co-dependence of HO-1 expression and tissue-resident macrophage differentiation.
authors.affiliation: Centre for Inflammation Research, Queen's Medical Research Institute, Little France Crescent, Edinburgh, UK. luke@devey.net
authors.forename: Luke
authors.initials: L
authors.lastname: Devey
authors.forename: David
authors.initials: D
authors.lastname: Ferenbach
authors.forename: Elodie
authors.initials: E
authors.lastname: Mohr
authors.forename: Kathryn
authors.initials: K
authors.lastname: Sangster
authors.forename: Christopher O
authors.initials: CO
authors.lastname: Bellamy
authors.forename: Jeremy
authors.initials: J
authors.lastname: Hughes
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Wigmore
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D000950:Antigens, Ly / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation; D005434:Flow Cytometry; D051547:Heme Oxygenase-1 / Q000378:metabolism* / Q000502:physiology; D007150:Immunohistochemistry; D007728:Kupffer Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D008099:Liver / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011971:Receptors, Immunologic / Q000378:metabolism; D015427:Reperfusion Injury / Q000276:immunology* / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D000950:Antigens, Ly; C494192:Ly-6C antigen, mouse; C092228:Marco protein, mouse; D011971:Receptors, Immunologic; C036763:monocyte-macrophage differentiation antigen; D051547:Heme Oxygenase-1