Growth characteristics of channel catfish ovary cells-influence of glucose and glutamine.
The growth characteristics and influence of glucose and glutamine on the growth and maintenance of channel catfish ovary (CCO) cells were investigated. Besides glutamine, amino acids threonine, arginine, methionine and serine were found to be essential for CCO cell growth. In the glucose-free medium, glutamine is utilized as energy source and no cell growth limitation was observed. However, the lack of glutamine in culture medium did not stimulate CCO cells to efficient glucose consumption. When both glucose and glutamine were deficient, cell growth was also observed suggesting no rigorous nutritional requirements. Obtained results are useful for further understanding of culture processes using CCO cells.
Slivac
Igor
I
Laboratory of Cell Culture Technology, Application and Biotransformation, Faculty of Food Technology and Biotechnology, University of Zagreb, 6 Pierotti St., 10000, Zagreb, Croatia.
Gaurina Srcek
Visnja
V
Radosević
Kristina
K
Porobić
Ivana
I
Bilić
Karmen
K
Fumić
Ksenija
K
Kniewald
Zlatko
Z