title: Real-time PCR for detecting circulating dengue virus in the Guangdong Province of China in 2006.
abstract: Dengue virus (DENV) causes a wide range of diseases in humans, from the acute febrile illness dengue fever (DF) to life-threatening dengue haemorrhagic fever/dengue shock syndrome. We developed four real-time quantitative PCR assays for each serotype of DENV based on computational analysis. These assays had high sensitivity and specificity without cross-reactivity for the four serotypes. To evaluate the performance of these assays in detecting and typing the virus in clinical samples, we analysed 64 serum samples from Guangdong during 2006. The results showed that 71 % of those samples were positive by the DEN-1 assay. The DENV assay results, in agreement with the serological tests and sequencing analysis, showed that the pathogen resulting in the DF explosion in Guangdong in 2006 belonged to DEN-1. Compared to the serological assays, the real-time PCR assays that we developed were much more sensitive in the 1-3 days after onset of the symptoms.
authors.affiliation: Guangzhou Medical Research Institute, Yan-Ling, Guangzhou 510507, PR China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Bai
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, PR China.
authors.forename: Licheng
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Biotechnology, Southwest University, Chongqing 400715, PR China.|Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, PR China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Tu
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100025, PR China.
authors.forename: Lisi
authors.initials: L
authors.lastname: Yao
authors.affiliation: Guangzhou Medical Research Institute, Yan-Ling, Guangzhou 510507, PR China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Guangzhou Medical Research Institute, Yan-Ling, Guangzhou 510507, PR China.
authors.forename: Boheng
authors.initials: B
authors.lastname: Tang
authors.affiliation: Guangzhou Medical Research Institute, Yan-Ling, Guangzhou 510507, PR China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Biotechnology, Southwest University, Chongqing 400715, PR China.
authors.forename: Yongji
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Guangzhou Medical Research Institute, Yan-Ling, Guangzhou 510507, PR China.
authors.forename: Meiyu
authors.initials: M
authors.lastname: Fang
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, PR China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002681:China / Q000453:epidemiology; D017931:DNA Primers; D003715:Dengue / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013045:Species Specificity
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; D007074:Immunoglobulin G; D007075:Immunoglobulin M