title: X-ray structure of the metal-sensor CnrX in both the apo- and copper-bound forms.
abstract: Both the X-ray structures of the apo- and the copper-bound forms of the metal-sensor domain (residues 31-148) of CnrX from Cupriavidus metallidurans CH34 were obtained at 1.74A resolution from a selenomethionine derivative. This four-helix hooked-hairpin is the first structure of a metal-sensor in an ECF-type signaling pathway. The copper ion is bound in a type 2-like center with a 3N1O coordination in the equatorial plane and shows an unprecedented remote fifth axial ligand with Met93 contributing a weak S-Cu bond. The signal onset cannot be explained by conformational changes associated with CnrX metallation.
authors.affiliation: Laboratoire des Protéines Membranaires, Institut de Biologie Structurale - Jean-Pierre Ebel, UMR 5075 CNRS-CEA-UJF, 41, rue Jules Horowitz, 38027 Grenoble Cedex, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Pompidor
authors.forename: Antoine P
authors.initials: AP
authors.lastname: Maillard
authors.forename: Eric
authors.initials: E
authors.lastname: Girard
authors.forename: Serge
authors.initials: S
authors.lastname: Gambarelli
authors.forename: Richard
authors.initials: R
authors.lastname: Kahn
authors.forename: Jacques
authors.initials: J
authors.lastname: Covès
mesh_terms: D000595:Amino Acid Sequence; D001059:Apoproteins / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D049921:Cupriavidus / Q000378:metabolism*; D019281:Dimerization; D008969:Molecular Sequence Data; D033903:Periplasmic Proteins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D001059:Apoproteins; D001426:Bacterial Proteins; D033903:Periplasmic Proteins; D003300:Copper