title: Activity of elaeochytrin A from Ferula elaeochytris on leukemia cell lines.
abstract: Phytochemical investigation of the roots of Ferula elaeochytris made it possible to isolate two sesquiterpene esters, 6-anthraniloyljaeschkeanadiol (elaeochytrin A) and 4beta-hydroxy-6alpha-(p-hydroxybenzoyloxy)dauc-9-ene (elaeochytrin B), as well as eight known compounds: 6-angeloyljaeschkeanadiol, teferidin, ferutinin, 6-(p-hydroxybenzoyl)epoxyjaeschkeanadiol, 6-(p-hydroxybenzoyl)lancerotriol, 5-caffeoylquinic acid, 1,5-dicaffeoylquinic acid and sandrosaponin IX. The cytotoxic activities of all compounds were investigated on K562R (imatinib-resistant) human chronic myeloid leukaemia and DA1-3b/M2(BCR-ABL) (dasatinib-resistant) mouse leukemia cell line. Elaeochytrin A was the most active compound on both cell lines (IC(50)=12.4 and 7.8microM, respectively). It was also tested on non-resistant human promyelocytic leukemia cells (HL60, IC(50)=13.1microM) and was not toxic to normal peripheral blood mononuclear cells up to 100microM.
authors.affiliation: Laboratoire de Pharmacognosie, E.A., Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille 2, Lille, France.
authors.forename: Racha
authors.initials: R
authors.lastname: Alkhatib
authors.forename: Thierry
authors.initials: T
authors.lastname: Hennebelle
authors.forename: Sami
authors.initials: S
authors.lastname: Joha
authors.forename: Thierry
authors.initials: T
authors.lastname: Idziorek
authors.forename: Claude
authors.initials: C
authors.lastname: Preudhomme
authors.forename: Bruno
authors.initials: B
authors.lastname: Quesnel
authors.forename: Sevser
authors.initials: S
authors.lastname: Sahpaz
authors.forename: François
authors.initials: F
authors.lastname: Bailleul
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D005309:Ferula / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000188:drug therapy*; D051379:Mice; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D012717:Sesquiterpenes / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D012717:Sesquiterpenes; C534909:elaeochytrin A