title: Phoenito experiments: combining the strengths of commercial crystallization automation.
abstract: The use of crystallization robots for initial screening in macromolecular crystallization is well established. This paper describes how four general optimization techniques, growth-rate modulation, fine screening, seeding and additive screening, have been adapted for automation in a medium-throughput crystallization service facility. The use of automation for more challenging optimization experiments is discussed, as is a novel way of using both the Mosquito and the Phoenix nano-dispensing robots during the setup of a single crystallization plate. This dual-dispenser technique plays to the strengths of both machines.
authors.affiliation: CSIRO Division of Molecular and Health Technologies, 343 Royal Parade, Parkville, VIC 3052, Australia. janet.newman@csiro.au
authors.forename: Janet
authors.initials: J
authors.lastname: Newman
authors.forename: Tam M
authors.initials: TM
authors.lastname: Pham
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Peat
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods; D003460:Crystallization / Q000295:instrumentation / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000737:chemistry; D012371:Robotics / Q000295:instrumentation / Q000379:methods
chemical_list: D011506:Proteins