Shared kinase fluctuations between two enzymatic reactions.
Kinases serve crucial roles in many cellular signaling pathways that process and transfer information. When signaling kinases phosphorylate two targets, these can serve as branch points that distribute information among two pathways. Responses to stimuli transmitted by activated kinases show high levels of cell-to-cell variation that influence cellular function. We ask how fluctuations around a steady state, due to kinase fluctuations and intrinsic noise, are distributed between two reactions with substrates phosphorylated by a shared kinase. We develop the formalism to answer this question and, for a realistic set of biological constants, we illustrate various features of fluctuations and relaxation times to a steady state. We find that the steady-state response determines the size and range in enzyme concentration of phosphorylated substrate fluctuations, and that the choice of an operating point can have a large impact on how shared kinase noise is distributed among two available pathways.
Viswanathan
G
G
Department of Neurology and Center for Translational Systems Biology, Mount Sinai School of Medicine, Box 1137, New York, NY 10029, USA.
Jayaprakash
C
C
Sealfon
S C
SC
Hayot
F
F
D000465:Algorithms; D020929:Mitogen-Activated Protein Kinase Kinases / Q000502:physiology; D008954:Models, Biological; D010766:Phosphorylation; D010770:Phosphotransferases / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D048490:raf Kinases / Q000502:physiology; D018631:ras Proteins / Q000502:physiology
D010770:Phosphotransferases; D048490:raf Kinases; D020929:Mitogen-Activated Protein Kinase Kinases; D018631:ras Proteins