Iron, ferritin and proteins of the methionine-centered redox cycle in young and old rat hearts.
Progressive oxidation of cellular components constitutes a major mechanism of the aging process. An emerging paradigm of redox signaling suggests that low level oxidants activate protective pathways resulting in prolonged cell survival. This report centers on the study of cardiac muscle in young and old rats, including (i) the expression of ferritin (Ft) the major iron storage protein, and (ii) the expression of the major proteins of the methionine-centered redox cycle (MCRC), which controls the cellular methionine redox status. Total amounts of Ft (protein) and its mRNA encoding for Ft L-subunit (Ft-L) were higher in the aged hearts, indicating that the iron-binding capacity of myocardial Ft increased with age. Among the proteins of the MCRC, methionine sulfoxide reductases A and B (MsrA, MsrB) and MsrA mRNA were significantly higher in hearts of old rats with a significant decrease in MsrA activity. The observed up-regulation of the expression of Msr and Ft-L could represent a protective response to the increased oxidative stress in the aging myocardium.
Bulvik
Baruch
B
Department of Cellular Biochemistry and Human Genetics, The Hebrew University of Jerusalem, Faculties of Medicine and Dental Medicine, 91120 Jerusalem, Israel.
Grinberg
Leonid
L
Eliashar
Ron
R
Berenshtein
Eddy
E
Chevion
Mordechai Mottie
MM
D000367:Age Factors; D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005260:Female; D005293:Ferritins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D007501:Iron / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology*; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism; D013879:Thioredoxins / Q000378:metabolism
D016877:Oxidants; D012333:RNA, Messenger; D013879:Thioredoxins; D005293:Ferritins; D008715:Methionine; D007501:Iron; D010088:Oxidoreductases; D013880:Thioredoxin-Disulfide Reductase; C488525:MsrA protein, rat