[Adaptive response on life span alteration in Drosophila melanogaster strains with mutations in heat shock factor and heat shock proteins genes].
The survival adaptive response (the life span alteration) induced by low-level radiation was studied in Drosophila using wild type strain (Canton-S) and strains mutant in genes of heat shock factor Hsf (1-4 alleles) and heat shock proteins (Hsp70Ba(304), Hsp83(e6A), Hsp22(EY09909)). The inductive doses of gamma-radiation were 6 and 60 cGy at a dose of 0.017 and 0.17 cGy/h. As a challenging dose we used feeding of flies by 20 mM paraquat in 5% sucrose solution during 24 h. The adaptive response was induced by chronic low dose irradiation in all experimental variants except Hsf4 and Hsp70Ba(304) homozygous strains. In Hsp22 homozygous strain the adaptive response was found only in males but wasn't in females. Thus the Drosophila strain homozygous on Hsp and Hsfmutations do not demonstrate the adaptive response in the most of studied variants. These results indicate the direct involvement of Hsp and Hsf genes to the formation of adaptive response.
Turysheva
E V
EV
Shaposhnikov
M V
MV
Moskalev
A A
AA
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D000483:Alleles; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D003627:Data Interpretation, Statistical; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics*; D008136:Longevity / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D008297:Male; D009154:Mutation; D010269:Paraquat / Q000633:toxicity; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; C053134:Hsf protein, Drosophila; D014157:Transcription Factors; D010269:Paraquat