Whole-cell recording using the perforated patch clamp technique.
Many ion channels, particularly potassium channels, are regulated by intracellular substances, such as nucleotides or Ca(2+). These modulators are washed out of the cell during standard whole-cell patch clamp recordings, or maintained at a particular concentration if they are included in the pipette solution. Perforated patch clamp recording permits electrical access between the cell and the patch pipette using pore-forming antibiotics such as nystatin or amphotericin B. These are permeable to small monovalent ions but present a physical barrier to the larger impermeable ions and molecules. This maintains the integrity of many cytoplasmic components including soluble second messengers, and also helps to prevent channel "run down".
Lippiat
Jonathan D
JD
Institute of Membrane and Systems Biology, University of Leeds, UK.
D000666:Amphotericin B / Q000494:pharmacology; D002460:Cell Line; D002468:Cell Physiological Phenomena; D004594:Electrophysiology / Q000379:methods; D006801:Humans; D007668:Kidney / Q000196:embryology; D008564:Membrane Potentials / Q000502:physiology; D009761:Nystatin / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*
D015221:Potassium Channels; D009761:Nystatin; D000666:Amphotericin B