Multiplex PCR for the discrimination of A. fumigatus, A. flavus, A. niger and A. terreus.
Aspergillus pathogens usually infect immunocompromised patients with lethal outcome. We report a multiplex PCR assay for the discrimination of the most frequent Aspergillus pathogens, A. fumigatus, A. flavus, A. niger and A. terreus, through distinct amplicons of 250 bp, 200 bp, 150 bp and 450 bp respectively, derived from the rDNA gene of A. terreus and the aspergillopepsin genes of the remaining species.
Logotheti
M
M
Department of Microbiology, Medical School, University of Athens, Greece. mlogotheti@pasteur.gr
Kotsovili-Tseleni
A
A
Arsenis
G
G
Legakis
N I
NI
D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology*; D001231:Aspergillus flavus / Q000235:genetics / Q000302:isolation & purification*; D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification*; D001234:Aspergillus niger / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004271:DNA, Fungal