title: Generation of mouse-human hybridomas secreting antibodies against peanut allergen Ara h1.
abstract: Two clones of mouse-human hybridomas, secreting human monoclonal antibodies to a peanut allergen Ara h1, were generated from human peripheral blood lymphocytes transformed with Epstein--Barr virus, followed by cell fusion with mouse myeloma cells. Epitope analysis with overlapping peptides synthesized on a multi-pin apparatus revealed antibody-binding sequences of Ara h1 protein.
authors.affiliation: National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan, shinmoto@affrc.go.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shinmoto
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Naganawa
authors.forename: Michie
authors.initials: M
authors.lastname: Shimmoto
authors.forename: Soheila J
authors.initials: SJ
authors.lastname: Maleki