[Mouse model of experimental antoimmune encephalomyelitisin C57BL/6J and expression of macrophage migration inhibitory factor].
OBJECTIVE
To detect the expression of macrophage migration inhibitory factor(MIF) in the brain and spinal cord of chronic non-remitting model of experimental autoimmune encephalomyelitis(EAE) mouse, and to discuss their effect on EAE/MS.


METHODS
Seventy-two female SPF C57BL/6J mice, aged 6k8 weeks, were randomly divided into an EAE group, a blank group, and an adjuvant group. The mice were immuned by mMOG35-55 and CFA. Immunohistochemic technique was used to detect the expression of MIF in the brain and spinal cord.


RESULTS
In the EAE group, we observed up-regulation of MIF of central nervous system(CNS) at onset, peak and chronic phase. During each phase, the difference of MIF between the EAE group and each of the other 2 groups was significant (P<0.05). In the EAE group, the expression of MIF was the highest at the peak, which was different from other periods (P<0.05).


CONCLUSIONS
MIF significanty expressed during the procedure of EAE disease, and may be related with the onset and exacerbation of EAE.
Gao
Yun-chun
YC
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.
Wang
Yu-zhong
YZ
Wang
Rui
R
Yan
Jin-jin
JJ
Zhou
Wen-bin
WB
D000818:Animals; D001921:Brain / Q000378:metabolism; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000378:metabolism*; D005260:Female; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D015854:Up-Regulation
D008263:Macrophage Migration-Inhibitory Factors