title: Association between von Willebrand factor gene polymorphism and preeclampsia.
abstract: OBJECTIVE
  The aims of this study were to assess whether the Msp I polymorphism of von Willebrand factor (vWF)(intron 19) gene differs between patients with preeclampsia and normal pregnancies in Chinese Han population, and to study the relationship between the Msp I polymorphism in the vWF(intron 19) gene and severity of preeclampsia symptoms within the preeclampsia group.
  
  
  METHODS
  In this case-control study, peripheral blood samples of 70 patients with preeclampsia and 82 normal pregnancies were genotyped for the Msp I polymorphism in vWF(intron 19) gene on the basis of a hospital-based study. The genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing techniques.
  
  
  RESULTS
  The genotype frequencies for M+/M+, M+/M- and M-/M- were 22.9, 45.7 and 31.4% in patients with preeclampsia, and 1.2, 36.6 and 62.2% in normal pregnancies, respectively. The frequency of the M+ allele in patients with preeclampsia was significantly higher than in normal pregnancies (45.7 vs. 19.5%).
  
  
  CONCLUSIONS
  The Msp I polymorphism in intron 19 (M+ allele) of vWF gene is associated with the development and the severity of preeclampsia, and may be a susceptibility factor for preeclampsia.
authors.affiliation: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, People's Republic of China.
authors.forename: Chengjuan
authors.initials: C
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Weiyuan
authors.initials: W
authors.lastname: Zhang
authors.forename: Song
authors.initials: S
authors.lastname: Yu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D014841:von Willebrand Factor / Q000235:genetics*
chemical_list: D014841:von Willebrand Factor