title: Spectroscopic study of 2-(2-pyridyliminomethyl)phenol as a novel fluorescent probe for superoxide anion radicals and superoxide dismutase activity.
abstract: A novel spectrofluorometric method, using 2-(2-pyridyliminomethyl)phenol as a fluorescent probe, was developed for the determination of superoxide anion radical (O(2) (*-)) and superoxide dismutase activity (SOD). The new fluorescent probe was synthesized and characterized with elemental analysis and IR spectra. It was oxidized by O(2) (*-) to form a less fluorescence product. Based on this reaction, a spectrofluorometric method was proposed and successfully used to determine superoxide anion radicals and SOD activity. The effects of interferences were studied. The reaction was simple, precise and sensitive. It was applied to determine SOD activity in garlic, papaya and spinach successfully.
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China.
authors.forename: Shuhui
authors.initials: S
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Mu
authors.forename: Jigang
authors.initials: J
authors.lastname: Gao
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000631:Aminopyridines / Q000138:chemical synthesis / Q000737:chemistry*; D002021:Buffers; D029441:Carica / Q000201:enzymology; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*; D005737:Garlic / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D007700:Kinetics; D010084:Oxidation-Reduction; D010636:Phenols / Q000138:chemical synthesis / Q000737:chemistry*; D011748:Pyrogallol / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis; D018724:Spinacia oleracea / Q000201:enzymology; D013482:Superoxide Dismutase / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013481:Superoxides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
chemical_list: C542481:2-(2-pyridyliminomethyl)phenol; D000631:Aminopyridines; D002021:Buffers; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010636:Phenols; D011748:Pyrogallol; D013481:Superoxides; D013482:Superoxide Dismutase