Synthesis of S-[13N]nitrosoglutathione (13N-GSNO) as a new potential PET imaging agent.
In the present paper, a fast and reproducible method for the synthesis of S-[(13)N]nitrosoglutathione is reported for the first time. The labeling strategy is based on the production of [(13)N]NO(3)(-) via the (16)O(p,alpha)(13)N nuclear reaction in water, as opposed to the standardized production of [(13)N]NH(4)(+) in 2mM aqueous ethanol. Following the reduction of [(13)N]NO(3)(-) to [(13)N]NO(2)(-), the reaction with glutathione in the presence of hydrochloric acid led to the desired radiotracer with a good radiochemical yield (24.2+/-2.0% end of synthesis, n=5) in a short production time (3min from the end of bombardment).
Llop
Jordi
J
Institut Alta Tecnologia PRBB - Fundació Privada, Parc de Recerca Biomèdica de Barcelona, C. Dr. Aiguader, 88, 08003 Barcelona, Spain.
Gómez-Vallejo
Vanessa
V
Bosque
Marta
M
Quincoces
Gemma
G
Peñuelas
Iván
I
D007553:Isotope Labeling / Q000379:methods*; D009590:Nitrogen Radioisotopes; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D026422:S-Nitrosoglutathione / Q000138:chemical synthesis; D012996:Solutions; D014867:Water
D009590:Nitrogen Radioisotopes; D019275:Radiopharmaceuticals; D012996:Solutions; D014867:Water; D026422:S-Nitrosoglutathione