Characterization of mice deficient in melanocortin 2 receptor on a B6/Balbc mix background.
We have previously reported that Melanocortin 2 receptor (MC2R(-/-)) deficient mice on B6 N5 generations exhibited macroscopically detectable adrenal glands with markedly atrophied zona fasciculata (zF) and lack of detectable levels of corticosterone, and reduced serum concentrations of aldosterone and epinephrine. All MC2R(-/-) mice on B6/N8 background die within 2 days after birth, while about half of the MC2R(-/-) mice on B6/Balbc mix background survived to adulthood. Both male and female MC2R(-/-) mice were fertile, suggesting that normal development and function of reproductive organs. MC2R(-/-) mice delivered from MC2R(-/-) dams failed to survive due to lung failure, suggesting that fetal or maternal corticosterone is essential for lung maturation. MC2R(-/-) mice failed to activate the hypothalamic-pituitary-adrenal axis in response to both immune and non-immune stimuli. MC2R(-/-) mice maintained glomerular structure and achieved electrolyte homeostasis by the activation of the renin-angiotensin-aldosterone system under low aldosterone and undetectable levels of corticosterone.
Chida
Dai
D
Department of Pathology, Research Institute, International Medical Center of Japan, 1-21-1, Toyama, Shinjuku-ku, Tokyo 162-8655, Japan. dchida@ri.imcj.go.jp
Sato
Tsuyoshi
T
Sato
Yoshinori
Y
Kubo
Mitsumasa
M
Yoda
Tetsuya
T
Suzuki
Harumi
H
Iwakura
Yoichiro
Y
D000818:Animals; D003345:Corticosterone / Q000097:blood; D005260:Female; D005298:Fertility; D015850:Interleukin-6 / Q000097:blood; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D008070:Lipopolysaccharides / Q000378:metabolism; D008168:Lung / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D044103:Receptor, Melanocortin, Type 2 / Q000235:genetics / Q000378:metabolism; D012083:Renin / Q000235:genetics / Q000378:metabolism; D012149:Restraint, Physical; D013315:Stress, Psychological; D015996:Survival Rate
D015850:Interleukin-6; D008070:Lipopolysaccharides; D044103:Receptor, Melanocortin, Type 2; D012083:Renin; D003345:Corticosterone