Inhibition of migration and invasion of colorectal cancer cells via deletion of Rac1 with RNA interference.
Cell migration and invasion are triggered by a number of chemoattractants that stimulate intracellular signaling pathways through regulating reorganization of the actin cytoskeleton. Rac1, an intracellular signal transducer, regulates a variety of cell functions, including the organization of the cytoskeleton, cell migration, and invasion. However, currently, very little is known about the roles of Rac1 in the cytoskeleton formation and invasion of human colorectal cancer cells. In our study, Rac1-shRNA was used to silence the Rac1 to reduce its expression specifically in Lovo cells. Our studies showed that RNA interference-mediated deletion of Rac1 strongly inhibited lamellipodia formation, cell migration, and invasion of Lovo cells in vitro. The deletion of Rac1 can serve as an alterative therapy to inhibit the invasion and metastasis of colorectal cancer cells.
Zhao
Shi-Yi
SY
Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, Guangzhou, 510515, Guangdong, China.
Sun
Yan
Y
Lai
Zhuo-Sheng
ZS
Nan
Qing-Zhen
QZ
Li
Kang
K
Zhang
Zhen-Shu
ZS
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D011554:Pseudopodia / Q000378:metabolism; D034622:RNA Interference; D017384:Sequence Deletion; D014162:Transfection; D020830:rac1 GTP-Binding Protein / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
D020830:rac1 GTP-Binding Protein