Electroencephalographic evidence of brainstem recruitment during scorpion envenomation.
Scorpion envenomation is a public health problem in Brazil, with most severe cases occuring in children under the age of 5 years (0.6% lethality). In fact, the toxic fractions of the Tityus serrulatus scorpion venom (TSSV) have greater permeability across the BBB of weanling rats when compared to adults. Although EEG alterations have been reported in up to 75% of pediatric severe cases, the role of the CNS in envenomation morbidity is still in debate. Our working hypothesis is that the neural substrates that play a major role in morbidity generate activity undetectable from EEG scalp leads. Twenty one-day-old rats (n=18) were injected s.c. with the deadliest toxic fraction of the TSSV, tityustoxin (TsTX; 2xDL50=6 mg/kg). EEG leads were stereotaxicaly implanted in the nucleus of the solitary tract (NTS) and left parietal cortex. EEG and ECG were continuously monitored by a video EEG system until death or for a maximum period of 240 min. An experimental group pre-treated with carbamazepine (CBZ) was added in order to better access the cause-effect relationship between neural discharges and the systemic ECG alterations. High amplitude discharges in the NTS, which correlated to cardiac alterations, were recorded soon after administration of TsTX. Abnormal electrographic activity spread throughout the cortex only later in the recording. As expected, the CBZ treatment increased the latency for the first epileptiform discharge, decreased EEG/ECG alterations and increased the general survival time. In summary: peripheral scorpion toxin inoculation recruits brainstem involved in cardiovascular control and initial electrographic activity was undetectable from the cortical electrode.
Guidine
Patrícia Alves Maia
PA
Núcleo de Neurociências, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, 31270 901 Belo Horizonte, Minas Gerais, Brazil.
Mesquita
Michel Bernanos Soares
MB
Moraes-Santos
Tasso
T
Massensini
André Ricardo
AR
Moraes
Márcio Flávio Dutra
MF
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001933:Brain Stem / Q000187:drug effects*; D002220:Carbamazepine / Q000494:pharmacology; D004562:Electrocardiography / Q000187:drug effects; D004569:Electroencephalography / Q000187:drug effects*; D008297:Male; D010296:Parietal Lobe / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012604:Scorpion Venoms / Q000037:antagonists & inhibitors / Q000633:toxicity*; D017552:Solitary Nucleus / Q000187:drug effects
D000927:Anticonvulsants; D012604:Scorpion Venoms; D002220:Carbamazepine; C003609:tityustoxin