title: In situ observation of a cell adhesion and metabolism using surface infrared spectroscopy.
abstract: In this study, we report on an in situ monitoring system of living cultured cells using infrared absorption spectroscopy in the geometry of multiple internal reflections (MIR-IRAS). In order to observe living cultured cells, the temperature in the sample chamber of a FT-IR spectrometer was maintained at 37 degrees C and a humidified gas mixture containing 5% CO(2) was introduced into the sample chamber. Human breast cell line MCF-7 cultured on Si MIR prisms were placed in the sample chamber and infrared spectra of MCF-7 cells were collected for 5 h. It was found that the adhesion and metabolism of MCF-7 cells could be monitored by the absorption intensity of amide-II protein band (1,545 cm(-1)) and also by the absorption intensities of CH( x ) bands (2,700-3,100 cm(-1)). These results suggest that our system is useful for a nondestructive and non-label monitoring of cell viability. Our method based on infrared absorption spectroscopy has a potential for bioscreening application.
authors.affiliation: Department of Electronic Engineering, Graduate School of Engineering, Tohoku University, 6-6-05 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8579, Japan.
authors.forename: Ko-Ichiro
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Parida
authors.initials: P
authors.lastname: Yamada
authors.forename: Ryo-Taro
authors.initials: RT
authors.lastname: Yamaguchi
authors.forename: Takami
authors.initials: T
authors.lastname: Muto
authors.forename: Ayumi
authors.initials: A
authors.lastname: Hirano
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kimura
authors.forename: Michio
authors.initials: M
authors.lastname: Niwano
authors.forename: Hiroko
authors.initials: H
authors.lastname: Isoda