Chiral ligand-exchange chromatography of amino acids using porous graphitic carbon coated with a dinaphthyl derivative of neamine.
In this paper, we describe the preparation and the evaluation of a porous graphitic carbon (PGC) column coated with a new dinaphthyl derivative of neamine for chiral ligand-exchange (LE) chromatography. It was shown that the graphitic surface/dinaphthyl anchor system efficiently (1.15 micromol/m(2)) and stably (three months of intensive use) adsorbs the neamine template onto the chromatographic support. The resulting coated PGC stationary phase showed appreciable LE-based enantioselective properties towards several native amino acids.
Zaher
Mustapha
M
Département de Pharmacochimie Moléculaire UMR 5063 CNRS, Institut de Chimie Moléculaire de Grenoble FR 2607, Université Grenoble I (Joseph Fourier), UFR de Pharmacie, 38041, Grenoble cedex 9, France.
Ravelet
Corinne
C
Baussanne
Isabelle
I
Ravel
Anne
A
Grosset
Catherine
C
Décout
Jean-Luc
JL
Peyrin
Eric
E
D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005601:Framycetin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D008024:Ligands; D015394:Molecular Structure; D016062:Porosity; D013237:Stereoisomerism; D013499:Surface Properties; D013997:Time Factors
C534898:3',6-di-O-2-methylnaphthalene-neamine; D000596:Amino Acids; D008024:Ligands; D005601:Framycetin; D006108:Graphite