The alpha-melanocyte stimulating hormone-related tripeptide K(D)PT stimulates human hair follicle pigmentation in situ under proinflammatory conditions.
BACKGROUND
Alpha-melanocyte stimulating hormone (alpha-MSH) is a well-tolerated immunomodulator with cytoprotective and anti-inflammatory effects that is known to stimulate melanogenesis and proliferation of follicular melanocytes. As human hair follicles (HFs) locally synthesize alpha-MSH, pharmacologically more easily handled alpha-MSH-related tripeptides, such as K(D)PT, may imitate this endogenous regulation, and may show a favourable side-effect profile on clinical use.


OBJECTIVE
To investigate the effect of the synthetic, alpha-MSH-related peptide K(D)PT [which is identical to interleukin (IL)-1beta(193-195)] on melanogenesis in human anagen HFs, under normal and proinflammatory growth conditions.


METHODS
Normal human anagen VI scalp HFs were microdissected and organ cultured with different concentrations of K(D)PT with or without coadministration of a proinflammatory, catagen-inducing stimulus, interferon (INF)-gamma. Masson-Fontana histochemistry and NKI/beteb immunohistochemistry were employed to assess changes in the degree of human HF pigmentation and melanocyte dendricity.


RESULTS
As confirmed by quantitative (immuno-)histomorphometry, compared with controls, K(D)PT alone did not affect human HF pigmentation in organ culture. However, in the presence of a strong, prototypic proinflammatory stimulus (IFN-gamma), K(D)PT significantly stimulated HF melanin content and melanocyte dendrite formation in situ.


CONCLUSIONS
The IL-1beta- and alpha-MSH-related tripeptide, K(D)PT, displays interesting hair pigmentation-stimulatory activities under proinflammatory conditions. These might become exploitable for innovative antigreying strategies, notably in postinflammatory poliosis (regrowth of white hair, e.g. during recovery from alopecia areata), where no effective clinical therapy is yet available.
Meyer
K C
KC
Department of Dermatology, University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany.
Brzoska
T
T
Abels
C
C
Paus
R
R
D000368:Aged; D000506:Alopecia Areata / Q000150:complications; D005260:Female; D006200:Hair Color / Q000187:drug effects; D018859:Hair Follicle / Q000187:drug effects / Q000378:metabolism*; D006728:Hormones / Q000009:adverse effects / Q000494:pharmacology*; D006801:Humans; D053583:Interleukin-1beta / Q000009:adverse effects*; D008875:Middle Aged; D009924:Organ Culture Techniques; D010858:Pigmentation / Q000187:drug effects; D012535:Scalp / Q000378:metabolism*; D000521:alpha-MSH / Q000009:adverse effects / Q000494:pharmacology*
D006728:Hormones; D053583:Interleukin-1beta; D000521:alpha-MSH