title: Selection of an effective red-pigment producing Monascus pilosus by efficient transformation with aurintricarboxylic acid.
abstract: The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction-enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Taiwan.
authors.forename: Yi-Pei
authors.initials: YP
authors.lastname: Chen
authors.forename: I-Ching
authors.initials: IC
authors.lastname: Chen
authors.forename: Ing-Er
authors.initials: IE
authors.lastname: Hwang
authors.forename: Gwo-Fang
authors.initials: GF
authors.lastname: Yuan
authors.forename: Li-Ling
authors.initials: LL
authors.lastname: Liaw
authors.forename: Ching-Ping
authors.initials: CP
authors.lastname: Tseng
mesh_terms: D000818:Animals; D001312:Aurintricarboxylic Acid / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006801:Humans; D032741:Monascus / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D010858:Pigmentation; D010957:Plasmids / Q000235:genetics; D011092:Polyethylene Glycols / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects; D014170:Transformation, Genetic / Q000187:drug effects*
chemical_list: D011092:Polyethylene Glycols; D001312:Aurintricarboxylic Acid; D004262:DNA Restriction Enzymes; D002122:Calcium Chloride