In vitro susceptibility of field isolates of Leishmania donovani to Miltefosine and amphotericin B: correlation with sodium antimony gluconate susceptibility and implications for treatment in areas of endemicity.
Indian Leishmania donovani isolates (n = 19) from regional zones representing various levels of antimony resistance displayed significantly (P < 0.01) correlated results with respect to in vitro susceptibility to the antileishmanial drugs sodium antimony gluconate, amphotericin B, and Miltefosine, raising the possibility of cross-resistance mechanisms operating in the field isolates. The results of gene expression analysis of LdMT and LdRos3 were suggestive of alternate mechanisms of Miltefosine susceptibility in the isolates.
Kumar
Dhiraj
D
Institute of Pathology, Safdarjung Hospital Campus, New Delhi, India.
Kulshrestha
Arpita
A
Singh
Ruchi
R
Salotra
Poonam
P
D000666:Amphotericin B / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000967:Antimony Sodium Gluconate / Q000494:pharmacology* / Q000627:therapeutic use; D000981:Antiprotozoal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D017931:DNA Primers; D004351:Drug Resistance; D006801:Humans; D007194:India / Q000453:epidemiology; D007893:Leishmania donovani / Q000187:drug effects* / Q000235:genetics; D007898:Leishmaniasis, Visceral / Q000188:drug therapy* / Q000469:parasitology; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000981:Antiprotozoal Agents; D017931:DNA Primers; D010767:Phosphorylcholine; C039128:miltefosine; D000666:Amphotericin B; D000967:Antimony Sodium Gluconate