Simultaneous determination of L-glutamate, acetylcholine and dopamine in rat brain by a flow-injection biosensor system with microdialysis sampling.
A flow-injection biosensor system with an on-line microdialysis sampling system is proposed for the simultaneous detection of neurotransmitters (L-glutamate, acetylcholine and dopamine) released from rat brain cells. The dialysate collected in the sample loop from the microdialysis probe was automatically injected into the flow-injection line with a triple electrode arranged perpendicular to the flow direction. The triple electrode was constructed by hybridizing a poly(1,2-diaminobenzene) film to two enzyme sensing-parts which respond to L-glutamate and acetylcholine, and by coating a Nafion film on a remaining sensing part which responds to dopamine, respectively, without any cross-reactivity. The three sensing parts of the triple electrode responded linearly to the concentrations of L-glutamate and acetylcholine in the range of 0.002-5 mM and to that of dopamine in the range of 0.002-20 mM, respectively, without any interference from oxidizable species present in the dialysate. The proposed flow-injection analytical method could be applied to an in vivo assay of these neurotransmitters released from rat-brain cells by the continuous KCl stimulation.
Yao
Toshio
T
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Naka, Sakai, Osaka 599-8531, Japan.
Okano
Gen
G
D000109:Acetylcholine / Q000032:analysis; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001921:Brain / Q000166:cytology; D001923:Brain Chemistry; D004298:Dopamine / Q000032:analysis; D004566:Electrodes; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis; D018698:Glutamic Acid / Q000032:analysis; D009474:Neurons / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000032:analysis*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats
D004800:Enzymes, Immobilized; D018377:Neurotransmitter Agents; D018698:Glutamic Acid; D011189:Potassium Chloride; D000109:Acetylcholine; D004298:Dopamine