Identification and characterization of a hypoallergenic ortholog of Ara h 2.01.
Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognized by approximately 90% of peanut hypersensitive individuals in the US. Because peanut has limited genetic variation, wild relatives are a good source of genetic diversity. After screening 30 Arachis duranensis accessions by EcoTILLing, we characterized five different missense mutations in ara d 2.01. None of these polymorphisms induced major conformational modifications. Nevertheless, a polymorphism in the immunodominant epitope #7 (S73T) showed a 56-99% reduction in IgE-binding activity and did not affect T cell epitopes, which must be retained for effective immunotherapy. The identification of natural hypoallergenic isoforms positively contributes to future immunological and therapeutic studies and peanut cultivar development.
Ramos
M Laura
ML
Department of Horticulture, University of Georgia, Tifton Campus, Tifton, GA 31793, USA.
Huntley
James J
JJ
Maleki
Soheila J
SJ
Ozias-Akins
Peggy
P
D055516:2S Albumins, Plant / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000595:Amino Acid Sequence; D010367:Arachis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D004252:DNA Mutational Analysis; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation / Q000235:genetics / Q000276:immunology; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D021183:Peanut Hypersensitivity / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D017433:Protein Structure, Secondary; D055314:Seed Storage Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D055516:2S Albumins, Plant; D016056:Immunodominant Epitopes; D055314:Seed Storage Proteins