title: Simultaneous detection of anti-HCV antibody and HCV core antigen.
abstract: HCV infection is usually diagnosed by means of an enzyme immune assay for the detection of antibody against HCV. The window period between infection and seroconversion remains a dramatic problem in the transfusional and diagnostic setting. In this chapter, we report (i) procedures for assays using two different approaches designed to reduce the window period and (ii) performance in terms of specificity and sensitivity in the detection of both antibody and antigen, and we compare their efficacy with that of commercial assays.
authors.affiliation: Department of Health Sciences, University of Genoa, Genoa, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Ansaldi
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Icardi
mesh_terms: D000818:Animals; D016174:Hepacivirus / Q000276:immunology* / Q000302:isolation & purification; D006526:Hepatitis C / Q000097:blood; D018937:Hepatitis C Antibodies / Q000097:blood* / Q000276:immunology*; D018936:Hepatitis C Antigens / Q000097:blood* / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D008163:Luminescent Measurements; D051379:Mice; D012680:Sensitivity and Specificity; D013997:Time Factors; D014758:Viral Core Proteins / Q000097:blood* / Q000276:immunology
chemical_list: D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens; D014758:Viral Core Proteins