Disruption of Myosin 1e promotes podocyte injury.
Myosin 1e (Myo1e) is one of two Src homology 3 domain-containing "long-tailed" type I myosins in vertebrates, whose functions in health and disease are incompletely understood. Here, we demonstrate that Myo1e localizes to podocytes in the kidney. We generated Myo1e-knockout mice and found that they exhibit proteinuria, signs of chronic renal injury, and kidney inflammation. At the ultrastructural level, renal tissue from Myo1e-null mice demonstrates changes characteristic of glomerular disease, including a thickened and disorganized glomerular basement membrane and flattened podocyte foot processes. These observations suggest that Myo1e plays an important role in podocyte function and normal glomerular filtration.
Krendel
Mira
M
Department Cell and Developmental Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA. krendelm@upstate.edu
Kim
Sangwon V
SV
Willinger
Tim
T
Wang
Tong
T
Kashgarian
Michael
M
Flavell
Richard A
RA
Mooseker
Mark S
MS
D000818:Animals; D001806:Blood Urea Nitrogen; D005919:Glomerular Filtration Rate; D007674:Kidney Diseases / Q000209:etiology; D007678:Kidney Glomerulus / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D024461:Myosin Type I; D009218:Myosins / Q000032:analysis / Q000502:physiology*; D050199:Podocytes / Q000737:chemistry / Q000473:pathology*
C505821:Myo1e protein, mouse; D024461:Myosin Type I; D009218:Myosins